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mass spectrometry
Product Specifications
The Thermo Scientific TSQ
Quantum Ultra triple quadrupole
mass spectrometer defines a
standard of excellence for your
quantitative analysis needs.
Thermo Scientific
TSQ Quantum Ultra/
Ultra AM/Ultra EMR
• Ultimate productivity for
bioanalytical analysis
• Universal Thermo Scientific Ion Max
source with Heated Electrospray
Ionization (HESI-II)
• Unmatched sensitivity and specificity
with H-SRM
• Supports 21 CFR Part 11 compliance
through Thermo Scientific LCQUAN
software
• 3000 amu mass range with
Thermo Scientific TSQ Quantum
Ultra EMR
• Fast positive/negative mode switching, ≤ 25 ms
The TSQ Quantum Ultra family’s innovative
technology provides wide linear dynamic
range and low sensitivity limits, defining a
high standard of excellence in mass spectral
quantitation. It features the revolutionary
Part of Thermo Fisher Scientific
Ion Max™ source with interchangeable
HESI-II, ESI and APCI probes, a titanium
skimmer with hard-edged skimming and
larger aperture for increased robustness and
sensitivity, and a redesigned ion transfer
tube lens with wider aperture for enhanced
ion focusing and ion transmission. The Ion
Max source also allows full probe adjustment in the x, y, and z directions. The probe
position can be optimized for maximum
robustness and sensitivity with any given
combination of flow rate, solvent, and ionization mode. Its new, ultra high temperature, self-cleaning APCI probe, with ceramic
heater, virtually eliminates carryover. All of
this comes with the benefits of constant
neutral loss scanning, parent scanning,
high-resolution precursor ion selectivity,
Selected Reaction Monitoring (SRM)
and Highly Selective Reaction Monitoring
(H-SRM).
TSQ Quantum Ultra / Ultra AM / Ultra EMR
The TSQ Quantum Ultra EMR™ offers
higher resolution, higher resolution precursor selection, and an Extended Mass Range
of up to 3000 daltons. This extended mass
range capability allows high-resolution
analysis of a whole new class of biopolymers including peptides, polysaccharides,
intact proteins, and oligonucleotides. It
combines the benefits of the TSQ Quantum
Ultra, including constant neutral loss
scanning, parent scanning, high-resolution
precursor ion selectivity, SRM and H-SRM
to deliver a complete solution for the
proteomics and large molecule research
community.
The Thermo Scientific TSQ Quantum
Ultra AM has the additional capability of
routine accurate mass measurements on the
chromatographic timescale. Its meticulously
engineered analyzer control circuitry takes
full advantage of the high-resolution
Thermo Scientific HyperQuad quadrupole
mass analyzers, enabling accurate mass
measurement capabilities that are easier to
use and have a greater dynamic range than
those of instruments based on
quadrupole/time-of-flight technology.
• Removable ion transfer tube provides
vent-free maintenance
• Integrated electron multiplier eliminates
field emission and microphonic noise
• High temperature, self-cleaning APCI
heater employing state-of-the-art ceramic
heater technology
• Digital electronic noise reduction
Hardware Features
Ion Max API Source
• Enhanced sensitivity and ruggedness
• Sweep gas reduces chemical noise
• Optimal 60-degree spray angle for best
sensitivity and ruggedness
• Interchangeable HESI-II, ESI, and APCI
ionization probes
• APPI/APCI combination probe
Typical TSQ Quantum Ultra
sensitivity for 0.5 pg chloramphenicol injected on a
Thermo Scientific Hypersil
GOLD aQ 20 X 2.1 mm 1.9 µm
particle packed column at a
flow rate of 500 µL/min 30:70
water/methanol. Operating in
negative ion mode monitoring
the transition 321.0152.1.
Three consecutive injections
on the column are visible
and showing excellent s/n
of better than 570.
• X, Y, and Z probe positioning adjustments
for all ionization probes
• Automatic source recognition for ease
of use and simplified data logging
• Dual square profile quadrupole ion guides
for the highest ion transmission
Triple Stage Quadrupole
• Dual HyperQuad™ precision hyperbolic
quadrupole mass analyzers for ultra
performance
Integrated Divert Valve
• Fully automated data system control
enables switching the solvent front,
gradient end point, or any portion of
the HPLC run to waste
• Automatic system optimization by loop
injections
• User-definable default state of the valve,
either “to waste-load”or “to source-inject”
Integrated Syringe Pump
• Automated infusion and loop injections
under full data system control
• Large 6-mm field radius provides high
transmission and superior peak shape
Source Options
• 90-degree square profile quadrupole ion
guide with noise-reducing geometry
• ESI probe compatible with liquid flow
rates of 1 µL/min to 1 mL/min, without
splitting
• Software control and automated
optimization of collision energy and
gas pressure
• Fully automated system calibration,
tuning, and compound optimization
• APPI/APCI combination probe compatible
with liquid flow rates of 50 µL/min to
2 mL/min, without splitting
Vacuum System
• APCI probe compatible with liquid flow
rates of 50 µL/min to 2 mL/min, without
splitting
• Four-stage differentially pumped vacuum
manifold
• Metal needle option for ESI with configurations for low-flow or high-flow analysis
• Advanced triple inlet turbomolecular
pump integrated with vacuum manifold
• NanoSpray source supports both static
and dynamic nanospray experiments,
compatible with liquid flow rates of
50 nL/min* to 50 µL/min
• Dual rotary vacuum pump configuration
Detection System
• Off-axis continuous dynode electron
multiplier with extended dynamic range
• Proprietary detection system uses a fast
switching (≤ 25 ms) post-acceleration
conversion dynode with ±10 kV applied
voltage
• HESI-II source compatible with liquid flow
rates of 1 µL/min to 2 mL/min, without
splitting
*Lower limit depends on gauge of needle used.
System Control
• Powerful embedded computer system
with Motorola PowerPC® processor
• Integrated Serial Peripheral Interconnect
(SPI) bus for reliable electronic communications between system modules
• Dedicated SHARC™ digital signal processor (DSP) for dedicated instrument control
• Fast 100BASE-T Ethernet port for PC-toinstrument communications
Data Acquisition
• Real-time, high-speed, digital signal
processing with dedicated SHARC digital
signal processor (DSP)
• Quantitation-Enhanced Data-Dependent
MS/MS (QED-MS/MS) for simultaneous
compound confirmation and quantitation
Accurate Mass Calibration
• Internal lock mass for best performance
• External lock mass for interference-free
measurement
• Post-acquisition processing
Data System
• Thermo Scientific Xcalibur processing and
instrument control software
• Data system control of all instrument
parameters
• High-speed analog-to-digital converter
(ADC) with 195 kHz sampling rate
• Superior comprehensive instrument
diagnostics
• Adjustable scan speeds to 5000 Da/sec
• Automated optimization of all instrument
parameters including gas pressures and
collision energy within an experiment
• High-resolution centroiding
Software Features
Scan Functions
• Highly sensitive full-scan MS in Q1 or Q3
• Selected Ion Monitoring (SIM) in Q1 or Q3
• Selected Reaction Monitoring (SRM) for
the most demanding bioanalytical assays
• Retention time correction in real time for
improved RSDs
• Product Ion Scanning
• Precursor Ion Scanning
• Neutral Loss Scanning
• H-SRM
• Reverse Energy Ramp MS/MS spectra
(RER) gives information rich MS/MS
spectra for solid compound identification
Advanced Experiments with DataDependent Acquisition Mode
• Available from all scan functions
• Thermo Scientific Dynamic Exclusion
allows acquisition of MSn spectra from
lower intensity ion species
• Polarity switching capabilities
• AutoSIM
• Direct control of multiple vendor LC
systems and autosampler configurations
through Xcalibur™ data system software
• High performance PC with Intel® Pentium®
microprocessor and Microsoft® Windows®
operating system
• 22-inch widescreen ultra-sharp flat panel
display monitor
Optional Thermo Scientific
Application-Specific Software
• LCQUAN™ quantitation software supports
21 CFR Part 11 compliance
• Mass Frontier™ – spectral interpretation
and classification software for the identification of unknowns
• MetWorks™ – automated metabolite identification, component detection and
predicted fragmentation in one intuitive
workplace
• QuickQuan™ – high-throughput, automated quantitation software for early drug
discovery
• QuickCalc™ software powered by Gubbs
Inc. GMSU – quantitation and reporting
solution for high-throughput ADME
laboratories
• Watson LIMS™ – highly specialized protocol-driven Laboratory Information
Management System designed to support
DMPK/Bioanalytical studies in drug
development
• Galileo LIMS™ – fully integrated system
for in vitro ADME experiments in a single
client-server application
• TraceFinder™ – simplifies method development and routine analysis in food
safety and environmental laboratories
• TSQ Module™ – works with industry-leading Watson™ LIMS to simplify the bioanalytical workflow in regulated laboratories
System Specifications
Sensitivity
Heated Electrospray (HESI) at Unit
Resolution
A 5 µL injection of a 100 fg/µL (0.3250
fmol/µL) reserpine solution on a Hypersil
GOLD aQ 20 X 2.1 mm 1.9 µm particle
packed column at a flow rate of 500 µL/min
30:70:0.1 water/Acetonitrile/Formic acid
will produce a minimum signal-to-noise
ratio of 150:1 for the transition of the protonated molecule at m/z 609.3 to the fragment ion at m/z 195.1 when operated in
selected reaction monitoring mode (SRM)
with Q1 and Q3 resolution set to 0.7 Da
FWHM.
Heated Electrospray (HESI) at High
Resolution
A 5 µL injection of a 100 fg/µL (0.3250
fmol/µL) reserpine solution on a Hypersil
GOLD aQ 20 X 2.1 mm 1.9 µm particle
packed column at a flow rate of 500 µL/min
30:70:0.1 water/Acetonitrile/Formic acid
will produce a minimum signal-to-noise
ratio of 150:1 for the transition of the protonated molecule at m/z 609.3 to the fragment ion at m/z 195.1 when operated in
selected reaction monitoring mode (SRM)
with Q1 resolution set to 0.2 Da FWHM and
Q3 resolution set to 0.7 Da FWHM.
Product Specifications
Atmospheric Pressure Chemical
Ionization (APCI) and Atmospheric
Pressure Photoionization (APPI) at Unit
Resolution
A 5 µL injection of a 100 fg/µL (0.3250
fmol/µL) reserpine solution on a Hypersil
GOLD aQ 20 X 2.1 mm 1.9 µm particle
packed column at a flow rate of 500 µL/min
30:70:0.1 water/Acetonitrile/Formic acid
will produce a minimum signal-to-noise
ratio of 150:1 for the transition of the protonated molecule at m/z 609.3 to the fragment ion at m/z 195.1 when operated in
selected reaction monitoring mode (SRM)
with Q1 and Q3 resolution set to 0.7 Da
FWHM.
APCI and APPI at High Resolution
A 5 µL injection of a 100 fg/µL (0.3250
fmol/µL) reserpine solution on a Hypersil
GOLD aQ 20 X 2.1 mm 1.9 µm particle
packed column at a flow rate of 500 µL/min
30:70:0.1 water/Acetonitrile/Formic acid
will produce a minimum signal-to-noise
ratio of 150:1 for the transition of the protonated molecule at m/z 609.3 to the fragment ion at m/z 195.1 when operated in
selected reaction monitoring mode (SRM)
with Q1 resolution set to 0.2 Da FWHM and
Q3 resolution set to 0.7 Da FWHM.
Mass Accuracy–
TSQ Quantum Ultra AM
Infusion of a mixture of polyethylene glycols
(PEGs) of average molecular weights 200,
400, 600, and 1000 at 50 pmoles/µL produces 27 ammoniated PEG ions from 124 to
1268 Da. Accurate mass data is generated
on each of the 25 ions from 168 to 1224 Da
using the neighboring peaks as internal lock
masses. The mass of each ion is determined
from the average of up to 100 scans and the
error between the expected mass and the
measured mass is expressed in mmu and
ppm. The root mean square (RMS) average
is computed from the errors of the 25 individual ions. The RMS error will be less than
or equal to 5 ppm.
Performance Specifications
Gas
Mass Range
• Collision gas: 99.995% pure Argon
• 10–1500 Da (TSQ Quantum Ultra/Ultra
AM)
• Collision gas supply pressure:
135 ± 70 kPa (20 ±10 psig)
• 10–3000 Da (TSQ Quantum Ultra EMR)
• Sheath/aux/sweep gas:
99% pure Nitrogen
Resolution
• 7500 (FWHM) at m/z 508 of polytyrosine
• Resolution is continuously adjustable to
better than 0.1 Da peak width (FWHM)
across the entire mass range.
Mass Stability
TSQ Quantum Ultra/Ultra EMR
• Mass assignment will be within ± 0.050
Da over a 24 hour period. The laboratory
room temperature must be maintained
between 15-27 °C (59-81°F). The optimum
temperature of operation is between
18-21°C (65-70 °F). The room temperature
may not change by more than 5 °C (9 °F)
during this period.
TSQ Quantum Ultra AM
• Mass assignment will be within ± 0.025
Da over a 24 hour period. The laboratory
room temperature must be maintained
between 15-27 °C (59-81°F). The optimum
temperature of operation is between
18-21°C (65-70 °F). The rate of change in
temperature may not exceed 2 °C per
hour (3.6 °F/hr) and not by more than 5 °C
(9 °F) during this period.
Installation Requirements
• Sheath/aux/sweep gas supply pressure:
690 ±140 kPa (100 ± 20 psig)
• Maximum sheath gas consumption:
~ 20 L/min.
Environment
• Functional temperature range:
15 °C to 27 °C (59 °F to 81°F)
• Optimal temperature range:
18 °C to 21°C (65 °F to 70 °F)
• TSQ Quantum Ultra heat output:
2,300 W (8,000 Btu/h)
• Particulate matter: < 3,500,000 particles
per cubic meter of air (< 100,000 particles
of > 5 µm diameter per cubic foot of air)
• Relative humidity:
20% to 80%, without condensation
• Floors must be free of vibration.
Dimensions
• TSQ Quantum Ultra family:
61 cm × 56 cm × 79 cm (h × w × d)
• Liquid chromatograph*:
61 cm × 76 cm × 61 cm (h × w × d)
• Minitower computer:
48 cm × 18 cm × 43 cm (h × w × d)
• Monitor: 46 cm × 51 cm × 18 cm (h × w × d)
Power
• One 230 Vac ±10%, 50/60 Hz at 16 A
minimum
• Four 120 Vac + 6-10%, 50/60 Hz at 20 A
or four 230 V ±10 %AC, 50/60 Hz at 13 A
• Earth ground hardwired to main panel
• Free from voltage variations above or
below the recommended operating range
• Forepumps (each): 30 cm × 20 cm × 64 cm
(h × w × d)
• Laser printer: 20 cm × 41 cm × 46 cm
(h × w × d)
Weight
• TSQ Quantum Ultra: 118 kg
• Liquid chromatograph*: 45 kg
• Minitower computer: 14 kg
• Monitor: 5 kg
• Forepumps (each): 34 kg
• Laser printer: 7 kg
*Values are for the Thermo Scientific Surveyor
LC system. Other LC systems will vary.
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