EASY-Spray Series Ion Source User Guide Revision C

EASY-Spray Series Ion Source User Guide Revision C

Thermo

EASY-Spray Series

Ion Source

User Guide

60053-97260 Revision C July 2015

© 2015 Thermo Fisher Scientific Inc. All rights reserved.

Acclaim, Accucore, EASY-nLC, EASY-Spray, EASY-Spray NG, Endura MD, Foundation, nanoViper, Optima,

PepMap, PepSwift, Q Exactive, TSQ Quantum Ultra, Velos Pro, and Viper are trademarks; Unity is a registered service mark; and Dionex, Exactive, LCQ, LTQ, Orbitrap, Orbitrap Fusion, Thermo Scientific,

TSQ Endura, TSQ Quantiva, TSQ Quantum, UltiMate, and Xcalibur are registered trademarks of Thermo

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IDEX is a registered trademark of IDEX Corporation. Microsoft and Windows are registered trademarks of

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All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

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The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser.

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Release history: Rev A, May 2012; Rev B, May 2013; Rev C, July 2015

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C

Contents

Thermo Scientific

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix

Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x

Compatible Mass Spectrometers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi

Product Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi

Contents of the EASY-Spray Series Ion Source Kits . . . . . . . . . . . . . . . . . . . . . xii

Cautions and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii

Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv

Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Advantages of Nanoelectrospray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Functional Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Source Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Controller Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Chapter 2 Installing the EASY-Spray Series Ion Source . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

Installing the Ion Source onto the Mass Spectrometer . . . . . . . . . . . . . . . . . . . . . 8

Connecting the Controller Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Connecting the EASY-Spray P-Bus Temp Control Cable . . . . . . . . . . . . . . . . . 13

Configuring the Venting Tee Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Adjusting the Emitter Tip Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Removing the EASY-Spray Series Source. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Chapter 3 Connecting the LCD Monitor and Video Camera. . . . . . . . . . . . . . . . . . . . . . . . . . .19

Connecting the Monitor and Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Adjusting the Video Picture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Chapter 4 Connecting the EASY-Spray Column Plumbing. . . . . . . . . . . . . . . . . . . . . . . . . . . .23

Tools and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Installing the EASY-Spray Column for the First Time. . . . . . . . . . . . . . . . . . . . 24

Using the nanoViper Fitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Connecting the LC to the EASY-Spray Column . . . . . . . . . . . . . . . . . . . . . . . . 27

Connecting an EASY-nLC 1000 Instrument to the EASY-Spray Column . . . 27

Connecting an EASY-nLC II Instrument to the EASY-Spray Column . . . . . 30

Connecting an RSLCnano System to the EASY-Spray Column. . . . . . . . . . . 33

EASY-Spray Series Ion Source User Guide

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Contents

Chapter 5 Configuring the Mass Spectrometer for NSI Mode . . . . . . . . . . . . . . . . . . . . . . . .37

Configuring the EASY-Spray NG Source’s NSI Parameters. . . . . . . . . . . . . . . . 37

Configuring the EASY-Spray Source’s NSI Parameters . . . . . . . . . . . . . . . . . . . 38

Selecting the Source for the LCQ Deca XP Max Mass Spectrometer . . . . . . . . . 40

Chapter 6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Preparing the Work Area. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Tools and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Cleaning the EASY-Spray Column Emitter Tip . . . . . . . . . . . . . . . . . . . . . . . . 43

Replacing the Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

Chapter 7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47

Chapter 8 Replaceable Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51

Spare Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55

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EASY-Spray Series Ion Source User Guide Thermo Scientific

F

Figures

Thermo Scientific

Figure 1.

nanoLC/MS system using the EASY-Spray ion source, camera, and monitor . . . . 1

Figure 2.

EASY-Spray NG source housing (P/N ES082) . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Figure 3.

EASY-Spray source housing (P/N ES081) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Figure 4.

Controller PCB located on the bottom of the source (P/N ES082) . . . . . . . . . . . 4

Figure 5.

Controller power supply assembly (shown with a European input plug) . . . . . . . 5

Figure 6.

Examples of the mass spectrometer ion sweep cones . . . . . . . . . . . . . . . . . . . . . . 9

Figure 7.

EASY-Spray NG locking lever positions (top view) . . . . . . . . . . . . . . . . . . . . . . . 9

Figure 8.

EASY-Spray locking lever positions (top view) . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Figure 9.

EASY-Spray NG source housing connection (ES082) . . . . . . . . . . . . . . . . . . . . 10

Figure 10.

EASY-Spray source housing connection (ES081) . . . . . . . . . . . . . . . . . . . . . . . . 11

Figure 11.

Power connection for the controller PCB (bottom view) . . . . . . . . . . . . . . . . . . 12

Figure 12.

Screw to remove before installing the Tee holder . . . . . . . . . . . . . . . . . . . . . . . 13

Figure 13.

Tee holder in the shipping configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Figure 14.

Emitter positioning tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Figure 15.

Gap positions in front of the mass spectrometer’s ion transfer tube (side view) . 15

Figure 16.

X- and z-axes position controls on the source (P/N ES081) . . . . . . . . . . . . . . . 16

Figure 17.

Y-axis position control (P/N ES082). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Figure 18.

Camera and column installed on the EASY-Spray source (P/N ES081) . . . . . . . 20

Figure 19.

EASY-Spray column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Figure 20.

nanoViper fitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Figure 21.

Recommended plumbing locations on the venting tee (nanoViper example) . . 28

Figure 22.

Tee holder secured to the bottom of the column holder . . . . . . . . . . . . . . . . . . 28

Figure 23.

Example of a one-column setup with nanoViper fittings. . . . . . . . . . . . . . . . . . 29

Figure 24.

Example of a two-column setup with nanoViper fittings and a Viper union . . . 30

Figure 25.

LC line with sleeve, ferrule, and nut . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Figure 26.

Column Out line with smaller fitting from the ZDV adapter union . . . . . . . . . 32

Figure 27.

Example of a two-column setup for the EASY-nLC II . . . . . . . . . . . . . . . . . . . 33

Figure 28.

Example of a one-column setup for an RSLCnano system (direct injection) . . . 34

Figure 29.

Example of a two-column setup for an RSLCnano system (preconcentration) . 35

Figure 30.

Ion Source page in the Tune window for the Orbitrap Fusion (example) . . . . . 38

Figure 31.

Tune Plus window for an NSI-configured Velos Pro (example) . . . . . . . . . . . . . 39

Figure 32.

NSI Source dialog box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

Figure 33.

Wash cap installed over the column emitter tip . . . . . . . . . . . . . . . . . . . . . . . . 43

Figure 34.

EASY-Spray with the new controller PCB and extra cover plate (bottom view). 44

Figure 35.

Controller PCB (P/N ES233) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

Figure 36.

Bottom of the EASY-Spray source without the controller PCB . . . . . . . . . . . . . 45

EASY-Spray Series Ion Source User Guide

vii

Figures viii

EASY-Spray Series Ion Source User Guide Thermo Scientific

P

Preface

The EASY-Spray Series Ion Source User Guide describes the hardware components, and provides installation and configuration procedures for the Thermo Scientific™ EASY-Spray™

Series ion sources.

Contents

Related Documentation

Compatible Mass Spectrometers

Product Requirements

Contents of the EASY-Spray Series Ion Source Kits

Cautions and Special Notices

Contacting Us

To suggest changes to the documentation

Complete a brief survey about this document by clicking the button below.

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Publications Editor at [email protected]

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Thank you in advance for your help.

Thermo Scientific EASY-Spray Series Ion Source User Guide

ix

Preface

Related Documentation

In addition to this guide, you can also access the Preinstallation Requirements Guide, Getting

Connected Guide, Getting Started Guide, and Hardware Manual for your specific

Thermo Scientific mass spectrometer as PDF files from the data system computer.

To view the product manuals

From the Microsoft™ Windows™ taskbar, choose Start > All Programs > Thermo

Instruments and so on.

To download user documentation from the Thermo Scientific website

1. Go to www.thermoscientific.com

.

2. In the Search box, type the product name and press Enter.

3. In the left pane, select Documents & Videos, and then under Refine By Category, click

Operations and Maintenance.

4. (Optional) Narrow the search results or modify the display as applicable:

• For all related user manuals and quick references, click Operator Manuals.

• For installation and preinstallation requirements guides, click Installation

Instructions.

• For documents translated into a specific language, use the Refine By Language feature.

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5. Download the document as follows: a. Click the document title or click Download to open the file.

b. Save the file.

x

EASY-Spray Series Ion Source User Guide Thermo Scientific

Preface

Compatible Mass Spectrometers

Use the EASY-Spray Series ion source

with the appropriate Thermo Scientific mass spectrometers (MSs) for nanoelectrospray (commonly referred to as nanoES or nanospray)

analysis. Table 1

lists several compatible MSs.

Table 1. Ion sources and compatible mass spectrometers

Ion source model Thermo Scientific mass spectrometer

EASY-Spray

• Orbitrap Fusion™ Series

• TSQ Endura™ and TSQ Quantiva™

• Exactive™ and Q Exactive™

• Orbitrap™ Series

• LTQ™ Series

• TSQ Quantum™ Ultra™

• LCQ™ Deca XP Max

For information about your specific mass spectrometer, refer to the instrument manuals.

Product Requirements

Table 2 lists the environmental requirements for the EASY-Spray Series ion source. However,

the requirements for the mass spectrometer supersede these requirements. Refer to the

“Operating Environment” chapter in the preinstallation requirements guide for your

Thermo Scientific mass spectrometer.

CAUTION The EASY-Spray Series source must connect to a certified Thermo Scientific mass spectrometer, which supplies high voltage capable of delivering a maximum of 8 kV and 100 μA.

If you connect the EASY-Spray Series source to another type of mass spectrometer, you might impair the protection provided by the equipment.

Table 2. EASY-Spray Series ion source environmental requirements

Parameter

Altitude

Humidity

Operating environment

Temperature

Specification

Up to 2000 m (6562 ft)

Maximum relative humidity of 80% for temperatures up to

31 °C (88 °F), decreasing linearly to 50% relative humidity at

40 °C (104 °F)

Pollution degree 2 (indoor environment; normally only nonconductive pollution)

Ambient temperature: 15–27 °C (59–81 °F)

Thermo Scientific EASY-Spray Series Ion Source User Guide

xi

Preface

Contents of the EASY-Spray Series Ion Source Kits

Table 3 lists the parts supplied in the EASY-Spray NG Ion Source Kit (P/N ES082) and the

EASY-Spray Ion Source Kit (P/N ES081), which are identical except for the source housing.

For a list of replaceable parts, see

Chapter 8, “Replaceable Parts.”

Table 3. Contents of the EASY-Spray Series ion source kits (Sheet 1 of 2)

Image Description

Provided in the EASY-Spray NG Ion Source Kit (P/N ES082) only

EASY-Spray NG housing

Quantity Part number

1 — a

Provided in the EASY-Spray Ion Source Kit (P/N ES081) only

EASY-Spray housing 1 — b

Actual temperature

C

35 50

30

OFF

C

60

55

Heater

EASY Spray

Provided in both ion source kits

EASY-Spray column, 15 cm × 3 μm ID 1 ES800

Controller power supply assembly:

• Power supply unit, 24 Vdc output

(shown without the input plug)

• Power adapter cable (not shown)

Dino-Lite™ digital video camera and power supply unit (not shown)

Monitor, power supply unit, and BNC adapter (not shown)

1 ES231

1 ES216

1 ES217

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EASY-Spray Series Ion Source User Guide Thermo Scientific

Preface

Table 3. Contents of the EASY-Spray Series ion source kits (Sheet 2 of 2)

Image Description

Tools:

• Emitter positioning tool

• 3 mm hex key

Quantity Part number

1 set ES232

1.5 mm hex key 1 —

A/B mixing/venting tee, stainless steel, with three attached ferrules and nuts

1 set SC901

Tee holder (for the mixing/venting tee) 1 ES234

Sleeves, PEEK™, 1/16 in. OD

Wash cap (for the EASY-Spray column)

3

1

IDEX™,

F-233

ES235 a

To order just the EASY-Spray NG source housing, you must order the EASY-Spray NG Ion Source Kit

(P/N ES082). b

To order just the EASY-Spray source housing, you must order the EASY-Spray Ion Source Kit (P/N ES081).

Cautions and Special Notices

Make sure that you follow the cautions and special notices presented in this guide. Cautions and special notices appear in boxes; those concerning safety or possible damage also have corresponding caution symbols.

This guide uses the following types of cautions and special notices.

CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION notice is accompanied by an appropriate CAUTION symbol.

Thermo Scientific EASY-Spray Series Ion Source User Guide

xiii

Preface

IMPORTANT Highlights information necessary to prevent damage to software, loss of data, or invalid test results; or might contain information that is critical for optimal performance of the system.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

The EASY-Spray Series Ion Source User Guide contains the following caution-specific symbols

( Table 4

).

Table 4. Caution-specific symbols and their meanings

Symbol Meaning

Chemical hazard: Wear gloves and other protective equipment, as appropriate, when handling toxic, carcinogenic, mutagenic, corrosive, or irritant chemicals. Use approved containers and proper procedures to dispose of waste oil and when handling wetted parts of the instrument.

Hot surface: Before touching, allow any heated components to cool.

Risk of electric shock: This instrument uses voltages that can cause electric shock. Before servicing, shut down the instrument and disconnect it from line power.

Risk of eye injury: Eye injury could occur from splattered chemicals or airborne particles. Wear safety glasses when handling chemicals or servicing the instrument.

Trip obstacle: Be aware of cords or other objects located on the floor.

xiv

EASY-Spray Series Ion Source User Guide Thermo Scientific

Preface

Contacting Us

There are several ways to contact Thermo Fisher Scientific for the information you need. You can use your smartphone to scan a QR code, which opens your email application or browser.

Contact us Customer Service and Sales Technical Support

(U.S.) 1 (800) 532-4752 (U.S.) 1 (800) 532-4752

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Thermo Scientific EASY-Spray Series Ion Source User Guide

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Preface xvi

EASY-Spray Series Ion Source User Guide Thermo Scientific

1

Introduction

The EASY-Spray Series ion source, shown in

Figure 1

, maintains excellent spray stability to ensure efficient evaporation and ionization of the liquid samples—the key to achieving the highest sensitivity at nano-flow rates.

Key benefits of the EASY-Spray Series are as follows:

• Simple and robust design for nano liquid chromatography (nanoLC) /MS integration

• Better separation with zero dead volume (ZDV) connections

• Increased productivity with the temperature-controlled EASY-Spray column™

Contents

Advantages of Nanoelectrospray

Functional Description

Figure 1.

nanoLC/MS system using the EASY-Spray ion source, camera, and monitor

Thermo Scientific

Thermo Scientific

EASY-nLC™ series instrument

Thermo Scientific mass spectrometer with the EASY-Spray ion source

EASY-Spray Series Ion Source User Guide

1

1

Introduction

Advantages of Nanoelectrospray

Advantages of Nanoelectrospray

The use of electrospray (ESI)

has evolved as a leading technique for generating intact, gas-phase ions from thermally labile, polar analytes in solution. In this technique, an emitter

(a capillary tube or needle) induces ionization at a controlled distance from a counter electrode. Direct current (dc) voltage is applied, either to the needle or to the solvent, to produce a strong electrical field at the emitter tip. The electric field excites the ions in the solution as they leave the emitter tip. This interaction results in electrohydrodynamic disintegration of the fluid, generation of droplets, and formation of an aerosol jet.

Conventional ESI employs flow rates from 1 μL/min to 1 mL/min. Expediting desolvation and droplet shrinkage often requires a drying gas, thermal heating, or both, due to the high volume of liquid that exits the emitter. Nanospray ionization (NSI), also known as

nanoelectrospray ionization (nanoESI or NSI)

, is a form of ESI that employs low rates of

10–1000 nL/min. NSI (or nanoESI) generally does not require a drying gas or thermal heating. Compared with ESI, NSI tolerates a wider range of liquid compositions including pure water.

As you lower the flow rate, a lower volume of mobile phase passes through the emitter, producing smaller aerosol droplets. This makes NSI more effective than conventional ESI at concentrating the analyte at the emitter tip, producing significant increases in sensitivity demonstrated by the signal response of the mass spectrometer.

Note The mass spectrometer’s instrument control application uses the terms nanospray and NSI.

Functional Description

This section describes the following principal components of the EASY-Spray Series source and their functions:

Source Housing

Controller PCB

Controller Power Supply

2

EASY-Spray Series Ion Source User Guide Thermo Scientific

1

Introduction

Functional Description

Source Housing

The EASY-Spray Series source housing ( Figure 2 or Figure 3

) arrives assembled and is easy to

install on the appropriate mass spectrometer (see Table 1 on page xi ). The source housing

includes two locking levers, two observation windows, a position-adjustable column holder, and a controller printed circuit board (PCB). For installation instructions, see

“Installing the

Ion Source onto the Mass Spectrometer” on page 8 .

Figure 2.

EASY-Spray NG source housing (P/N ES082)

Opening for the camera

Source housing

Column holder

Figure 3.

EASY-Spray source housing (P/N ES081)

Opening for the camera

Source housing

Column holder

Thermo Scientific EASY-Spray Series Ion Source User Guide

3

1

Introduction

Functional Description

Using the top and front observation windows, you can view the tip of the emitter positioning tool while you move it into position. To enhance your view of the tool and then later of the emitter, connect the digital video camera and LCD monitor. For instructions, see

Chapter 3,

“Connecting the LCD Monitor and Video Camera.”

The column holder is mounted onto the front observation window and includes a position control knob. Use the control knob to align the z axis (front-to-back) of the emitter positioning tool (

Figure 14 on page 15 ) with the mass spectrometer’s ion transfer tube. To

align the tool’s x-axis (side-to-side) and y-axis position (up-and-down), use the controls that are built into the EASY-Spray Series housing. For instructions, see

“Adjusting the Emitter Tip

Position” on page 15 .

Controller PCB

The EASY-Spray Series source contains a controller PCB that powers and controls the heat generated for the connected EASY-Spray column. The controller PCB includes an input power socket, an output heater socket, a temperature control dial, and an actual temperature display. The bottom panel for the EASY-Spray NG source (

Figure 4

) is slightly different from that of the EASY-Spray source.

Figure 4.

Controller PCB located on the bottom of the source (P/N ES082)

Actual temperature display

Controller PCB (not visible)

Input power connection

The controller power supply unit connects to the input power socket (labeled P-BUS), which is located on the bottom right side of the EASY-Spray Series source. The heater cable from the

EASY-Spray column connects to the heater socket, which is located on the front and labeled

“Heater.”

The temperature control dial has an OFF setting and temperature settings in 5 °C increments from 30 through 60 °C. As the column heats up, the actual temperature appears on the display.

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EASY-Spray Series Ion Source User Guide Thermo Scientific

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Introduction

Functional Description

Tip If you have a Thermo Scientific EASY-nLC 1000 or EASY-nLC II instrument and the optional EASY-Spray P-Bus Temp Control Cable a

(P/N 70005-63055), you can connect this cable between the LC instrument and the EASY-Spray Series source to power the source and enable touch-screen control of the EASY-Spray column’s temperature.

For cable installation instructions, see “Connecting the EASY-Spray P-Bus Temp Control

Cable” on page 13 . For information about controlling the column temperature through

the LC instrument’s touch screen or the Xcalibur Instrument Setup window, refer to the

EASY-nLC Series instrument manuals. a

For use with the touch-screen application version 3.2 and later

Controller Power Supply

Figure 5

shows the controller power supply assembly that consists of a wall-mounted power supply unit with a detachable input plug and a power adapter cable. The other end of the power adapter cable connects to the input power socket (labeled P-BUS) located on the bottom of the EASY-Spray Series source to provide the 24 Vdc input for the controller PCB.

Figure 5.

Controller power supply assembly (shown with a European input plug)

Power supply unit with output cord

Power adapter cable

The total length of the output cord and power adapter cable is approximately 274 cm (9 ft).

Make sure that an electrical wall outlet is within reach for the power supply unit and that you provide suitable clearance around it for cooling.

Thermo Scientific EASY-Spray Series Ion Source User Guide

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1

Introduction

Functional Description

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EASY-Spray Series Ion Source User Guide Thermo Scientific

2

Installing the EASY-Spray Series Ion Source

Follow these procedures to install or remove the EASY-Spray Series ion source. The only tool required is the 3 mm hex key.

Note The complete EASY-Spray Series source installation, including the digital camera and LCD monitor, requires three electrical wall outlets. Ensure that your lab has the appropriate outlets near the nanoLC/MS system.

Contents

Installing the Ion Source onto the Mass Spectrometer

Connecting the Controller Power Supply

Configuring the Venting Tee Holder

Adjusting the Emitter Tip Position

Removing the EASY-Spray Series Source

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Installing the EASY-Spray Series Ion Source

Installing the Ion Source onto the Mass Spectrometer

Installing the Ion Source onto the Mass Spectrometer

Before you install the EASY-Spray Series source, follow the procedure to prepare the mass spectrometer.

To prepare the mass spectrometer

1. If any other source is installed, remove it from the mass spectrometer after it has cooled to room temperature.

For instructions, refer to the mass spectrometer’s documentation.

CAUTION Hot surface. Accidental contact with the part of the API probe or spray insert located inside the housing can severely burn you, as this area of the part can reach 350 °C (662 °F). In addition, although the exterior surface of the housing and the exposed part of the probe or spray insert fall below the limit of 70 °C (158 °F) for accessible metal surfaces, they too pose a burn hazard. Therefore, handle ALL heated parts with extreme caution.

• Before removing the API probe, spray insert, or housing, allow the part to cool to room temperature (approximately 20 minutes) before you touch it.

• If the mass spectrometer connects to an LC system, leave the solvent flow from the LC pump on while the probe or spray insert cools to room temperature.

2. If the

ion sweep cone (

Figure 6 ) is installed, remove it from the mass spectrometer by

grasping the outer ridges of the ion sweep cone and pulling it off.

The spray cone is directly behind the ion sweep cone ( Figure 9 on page 10 or

Figure 10 on page 11

).

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EASY-Spray Series Ion Source User Guide Thermo Scientific

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2

Installing the EASY-Spray Series Ion Source

Installing the Ion Source onto the Mass Spectrometer

Figure 6.

Examples of the mass spectrometer ion sweep cones

Ion sweep cone for the Orbitrap Fusion mass spectrometer

Ion sweep cone for the legacy mass spectrometers (shown is the offset orifice type for ESI, H-ESI, and APCI)

To install the source onto the mass spectrometer

1. Follow the procedure “To prepare the mass spectrometer” on page 8

.

2. Depending on your source type, unlock the source’s locking levers as follows:

• For the EASY-Spray NG source, rotate the locking levers until they are horizontal

( Figure 7 ).

Figure 7.

EASY-Spray NG locking lever positions (top view)

Unlocked (horizontal) position

Locked

(vertical) position

EASY-Spray Series Ion Source User Guide

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2

Installing the EASY-Spray Series Ion Source

Installing the Ion Source onto the Mass Spectrometer

–or–

• For the EASY-Spray source, rotate the locking levers toward the front of the source

(right lever in

Figure 8

).

Figure 8.

EASY-Spray locking lever positions (top view)

Locked position Unlocked position

Top observation window

3. Do one of the following:

• For the EASY-Spray NG source, align the three guide pins on the back of the source with the guide pin holes on the front of the mass spectrometer (

Figure 9

).

Figure 9.

EASY-Spray NG source housing connection (ES082)

MS spray cone

High voltage (HV) connector

Spring-loaded bolt delivers HV to the column

Guide pins on the MS (for example, Orbitrap

Fusion) ion source mount assembly

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EASY-Spray Series Ion Source User Guide

Guide holes on the back of the

EASY-Spray NG source housing

Thermo Scientific

Thermo Scientific

2

Installing the EASY-Spray Series Ion Source

Installing the Ion Source onto the Mass Spectrometer

–or–

• For the EASY-Spray source, align the two guide pin holes on the back of the source

with the guide pins on the front of the mass spectrometer ( Figure 10

).

Note Your legacy mass spectrometer might have a slightly different mount assembly, ion sweep cone, or both.

Figure 10. EASY-Spray source housing connection (ES081)

MS spray cone HV connector Spring-loaded bolt delivers HV to the column

Guide pins on the legacy MS ion source mount assembly

Guide pin holes on the back of the

EASY-Spray source housing

4. Carefully press the source onto the mass spectrometer.

5. Lock the source’s locking levers.

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Installing the EASY-Spray Series Ion Source

Connecting the Controller Power Supply

Connecting the Controller Power Supply

If the LC instrument includes the EASY-Spray P-Bus Temp Control Cable

(P/N 70005-63055), you can skip this section and go to

Connecting the EASY-Spray P-Bus

Temp Control Cable .

CAUTION To avoid an electric shock, always use the detachable input plug that is appropriate for your country or region and comes with the power supply unit.

To connect the controller power supply unit

1. Turn the temperature dial on the EASY-Spray Series source to the OFF position.

2. If not already connected, connect the detachable input plug and power adapter cable to the power supply unit (

Figure 5 on page 5 ).

3. Plug the power supply unit into an electrical wall outlet.

4. Connect the power adapter cable to the P-BUS socket located on the bottom, right side of the source (

Figure 17 on page 17 ) as follows:

a. Align the red dot on the plug with the red dot on the socket, which is on the right side of the socket (

Figure 11

).

Figure 11. Power connection for the controller PCB (bottom view)

Red dot on the socket

Red dot on the plug b. Push the plug upward into the socket.

CAUTION After completing the power supply connections, route the output cord so that it is not a trip hazard.

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Installing the EASY-Spray Series Ion Source

Connecting the EASY-Spray P-Bus Temp Control Cable

Connecting the EASY-Spray P-Bus Temp Control Cable

If you have the optional EASY-Spray P-Bus Temp Control Cable (P/N 70005-63055) and an

EASY-nLC 1000 or EASY-nLC II instrument, use this cable to control the EASY-Spray column temperature and provide power to the EASY-Spray Series source. This cable replaces the use of the controller power supply unit.

To connect the EASY-Spray P-Bus Temp Control Cable

1. Connect one end of the cable to the P-BUS socket on the back of the LC instrument.

2. Connect the other end of the cable to the P-BUS socket on the bottom of the source.

Configuring the Venting Tee Holder

Tip The EASY-Spray NG ion source (ES082) ships with the Tee holder attached to the column holder. If you have the EASY-Spray ion source (ES081), you can order the

optional Tee holder to support the venting tee; see Chapter 8, “Replaceable Parts.”

Use this procedure to configure the (venting) Tee holder before inserting the emitter positioning tool or the EASY-Spray column.

Follow these procedures, as applicable:

To install the optional Tee holder under the column holder

To configure the Tee holder

To install the optional Tee holder under the column holder

1. Remove the screw located on the bottom of the column holder (

Figure 12

).

Figure 12. Screw to remove before installing the Tee holder

Thermo Scientific

Remove this screw.

EASY-Spray Series Ion Source User Guide

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2

Installing the EASY-Spray Series Ion Source

Configuring the Venting Tee Holder

2. Hold the Tee holder (

Figure 13 ) under the column holder with one hand. With the other

hand, insert and tighten the hex socket-head bolt through the bottom of the Tee holder and the column holder.

The bolt is provided with the Tee holder.

Figure 13. Tee holder in the shipping configuration

This end goes below the column holder.

Sliding lock

Hex socket-head bolt

To configure the Tee holder

1. Bend the top part of the Tee holder down to the extended position ( Figure 22 on page 28 ).

2. Move the sliding lock toward the column holder.

Note If the ion source is not to be used for an extended period of time, unlock and position the Tee holder as shown in

Figure 13

.

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EASY-Spray Series Ion Source User Guide Thermo Scientific

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Installing the EASY-Spray Series Ion Source

Adjusting the Emitter Tip Position

Adjusting the Emitter Tip Position

Before installing the EASY-Spray column, use the emitter positioning tool ( Figure 14 ) and

3 mm hex key to adjust the position of the column holder.

Figure 15 shows the emitter positioning tool touching the mass spectrometer’s ion transfer

tube. The length of the tool’s tip ensures the 3 mm gap for the EASY-Spray column’s emitter.

Figure 14. Emitter positioning tool

Figure 15. Gap positions in front of the mass spectrometer’s ion transfer tube (side view)

0 mm gap between the ion transfer tube and the emitter positioning tool

Emitter positioning tool

Mass spectrometer spray cone

3 mm gap between the ion transfer tube and an actual emitter

EASY-Spray column

(shown without its cable)

Ion transfer tube

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Installing the EASY-Spray Series Ion Source

Adjusting the Emitter Tip Position

To adjust the emitter tip position

Tip To enhance your view of the emitter positioning tool’s tip, install the video camera and monitor before you begin this procedure. For instructions, see

Chapter 3,

“Connecting the LCD Monitor and Video Camera.”

1. Retract the z-axis (front-to-back) position by turning the control knob located on the

column holder counterclockwise ( Figure 16 ).

2. Insert the emitter positioning tool into the top of the column holder, and then slowly slide it forward until it clicks into place.

CAUTION High voltage. To prevent electric shock, never insert a foreign object into the source opening, which is intended for the emitter positioning tool and the

EASY-Spray column only.

3. Align the x-axis (side-to-side) position of the emitter positioning tool to the bore of the ion transfer tube by inserting the hex key into the left-side hex socket-head screw

( Figure 16

).

Figure 16. X- and z-axes position controls on the source (P/N ES081)

Inserting the emitter positioning tool

Z-axis position control (knob)

X-axis position control

(hex socket-head screw)

Y

Z

X

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Installing the EASY-Spray Series Ion Source

Removing the EASY-Spray Series Source

4. Align the y-axis (up-and-down) position of the emitter positioning tool to the bore of the ion transfer tube by inserting the hex key into the bottom hex socket-head screw

( Figure 17

).

Figure 17. Y-axis position control (P/N ES082)

Y-axis position control

One of the hex socket-head screws for the controller PCB (P/N ES233)

5. Adjust the z-axis (front-to-back) position of the emitter positioning tool’s tip by turning

the control knob located on the front of the column holder ( Figure 16 on page 16

). Move the tool’s tip so that it gently touches the bore of the ion transfer tube.

IMPORTANT The fused-silica emitter is fragile. Make sure that the emitter positioning tool gently touches the ion transfer tube when you adjust its position or you will damage the actual emitter when fully inserted, requiring you to replace the

EASY-Spray column.

6. After you adjust the tool tip’s position, slowly remove the emitter positioning tool.

The position of the column holder is now calibrated for use with any EASY-Spray column.

You must repeat this procedure if you later change the x-, y-, or z-axis position.

Removing the EASY-Spray Series Source

Follow this procedure if you must remove the EASY-Spray Series ion source from the mass spectrometer.

To remove the source from the mass spectrometer

1. In the applicable instrument control application, place the mass spectrometer in Standby mode.

For instructions, refer to the mass spectrometer’s documentation.

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Installing the EASY-Spray Series Ion Source

Removing the EASY-Spray Series Source

2. Turn the source’s temperature dial to the OFF position.

CAUTION Hot surface. The maximum safety limit for heated surfaces is 70 °C

(158 °F). Although the surface temperature of the EASY-Spray Series ion source falls below this maximum, it can still severely burn you, reaching approximately 50 °C

(122 °F) with the heater unit turned on. Allow the source to cool to room temperature (approximately 20 minutes) before you touch it.

3. Turn off the LC instrument.

4. Disconnect the LC plumbing from the EASY-Spray column, and then remove the

EASY-Spray column.

Tip Do not pull on the cable from the EASY-Spray column. Use the plug to disconnect the column from the heater socket.

5. If the Tee holder is installed, move the sliding lock away from the column holder, and then bend the unattached end upward (

Figure 13 on page 14 ).

6. Remove the camera as follows: a. Unplug the camera power supply unit from the electrical wall outlet. b. Disconnect the camera’s yellow video connector from the back of the monitor and the black power connector from the camera power supply unit. c.

Using the 1.5 mm hex key, loosen the hex socket-head screw securing the camera, and then remove the camera.

7. Do one of the following, as applicable:

• Disconnect the EASY-Spray P-Bus Temp Control Cable from the source.

• Unplug the controller power supply unit from the electrical wall outlet, and then disconnect the output cable from the source.

8. Unlock the source’s locking levers (

Figure 7 on page 9 and

Figure 8 on page 10

).

9. Grasp the source housing with both hands and slowly pull it away from the mass spectrometer.

After you remove the EASY-Spray Series ion source from the mass spectrometer, place it in its original shipping box. The EASY-Spray Series source does not require cleaning.

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EASY-Spray Series Ion Source User Guide Thermo Scientific

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Connecting the LCD Monitor and Video Camera

Follow these procedures to connect the LCD monitor and digital video camera, and to adjust the video picture. The only tool required is the 1.5 mm hex key.

Contents

Connecting the Monitor and Camera

Adjusting the Video Picture

Connecting the Monitor and Camera

To enhance your view of the emitter tip, install the provided LCD monitor and digital video camera (see

Table 3 on page xii

).

To connect the monitor and camera

1. Follow the procedure “To install the source onto the mass spectrometer” on page 9 .

2. Plug the two power supply units into electrical wall outlets.

3. Install the camera as follows: a. Insert the camera into the opening above the top observation window with the focusing wheel facing toward you (

Figure 18

). b. Using the 1.5 mm hex key, tighten the hex socket-head screw on the left side of the

top observation window ( Figure 16 on page 16

) until it touches the camera—do not overtighten the screw. c.

Connect the camera’s black power connector to its power supply unit.

Thermo Scientific EASY-Spray Series Ion Source User Guide

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3

Connecting the LCD Monitor and Video Camera

Connecting the Monitor and Camera

Figure 18. Camera and column installed on the EASY-Spray source (P/N ES081)

Camera light button

Camera focusing wheel

Hex socket-head screw to secure the camera

EASY-Spray column

4. On the back of the monitor, make the following connections:

• BNC adapter to one of the Video IN ports

• Camera’s yellow video connector to the BNC adapter

• Output cord from the other power supply unit to the DC 12V IN socket

CAUTION After completing the power supply connections, route the output cords so that they are not a trip hazard.

5. Place the monitor on top of the mass spectrometer near the source by using its support arm.

Make sure that the monitor is not too close to the edge of the instrument.

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EASY-Spray Series Ion Source User Guide Thermo Scientific

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Connecting the LCD Monitor and Video Camera

Adjusting the Video Picture

Adjusting the Video Picture

Use this procedure to focus the picture from the video camera. For additional information, refer to the manuals for the monitor and camera.

To adjust the video picture

1. Follow the procedure “To connect the monitor and camera” on page 19 .

2. Turn on the monitor by pressing its POWER button.

The picture appears after a few seconds and is probably blurred. If there is no picture, press the monitor’s SOURCE button to change to the other video input channel.

3. If the picture is too dark, press the light button at the top of the camera (

Figure 18 on page 20 ).

4. Adjust the picture by using the camera’s focusing wheel ( Figure 18 on page 20

).

Thermo Scientific EASY-Spray Series Ion Source User Guide

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3

Connecting the LCD Monitor and Video Camera

Adjusting the Video Picture

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EASY-Spray Series Ion Source User Guide Thermo Scientific

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Connecting the EASY-Spray Column Plumbing

Follow these procedures to connect the plumbing from the Thermo Scientific LC instrument to the EASY-Spray column.

Contents

Tools and Supplies

Installing the EASY-Spray Column for the First Time

Using the nanoViper Fitting

Connecting the LC to the EASY-Spray Column

Tools and Supplies

Table 5 lists the required tools and supplies for configuring the LC plumbing and EASY-Spray

column connections. Not all parts are required.

Note The kit for the EASY-Spray Series ion source supplies the venting tee, fittings, and sleeves only. You are responsible for supplying the remaining parts and tools.

Table 5. Tools and supplies (Sheet 1 of 2)

Image Description

One- and two-column setups

A/B mixing/venting tee, stainless steel, with three attached ferrules and nuts

Sleeves, PEEK, 1/16 in. OD

Part number

SC901

IDEX, F-233

Wrench, open end, 1/4 in. —

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Connecting the EASY-Spray Column Plumbing

Installing the EASY-Spray Column for the First Time

Table 5. Tools and supplies (Sheet 2 of 2)

Image

Description Part number

Gloves, lint-free and powder-free Fisher Scientific™ 19-120-2947 a

Unity SM Lab Services :

• 23827-0008 (size medium)

• 23827-0009 (size large)

Two-column setup

Any nanoViper™-equipped precolumn

This image shows the Acclaim™

PepMap100 Nano-Trap.

(For the PepMap100

Nano-Trap) Fisher Scientific,

164705

Viper™ union

(for use with the EASY-nLC 1000)

ZDV adapter union (1/16 to

1/32 in.) and plug (not shown)

(for use with the EASY-nLC II)

UltiMate™ 3000 RSLCnano

EASY-Spray Application Kit a

Multiple sizes are available.

Fisher Scientific 6040.2304

IDEX, P-881

Thermo Scientific Dionex™,

6720.0395

Installing the EASY-Spray Column for the First Time

The EASY-Spray column ( Figure 19 ) contains an integrated heatable column and a

fused-silica emitter. For available columns, see Chapter 8, “Replaceable Parts.”

IMPORTANT The EASY-Spray column assembly includes an emitter protective cover. If you push back this cover by hand, you could damage the fused-silica emitter and make the

EASY-Spray column unusable.

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EASY-Spray Series Ion Source User Guide Thermo Scientific

Figure 19. EASY-Spray column

4

Connecting the EASY-Spray Column Plumbing

Installing the EASY-Spray Column for the First Time nanoViper fitting for the

LC connection

Emitter protective cover

Protective cap

Thermo Scientific

Heater cable and plug

To install the EASY-Spray column

1. If the Tee holder is attached to the column holder, follow the procedure “To configure the

Tee holder” on page 14 .

2. Follow the procedure “To adjust the emitter tip position” on page 16 .

Note You can skip this step after the first time if you have not adjusted the column holder’s position.

3. Remove the protective cap on the end of the EASY-Spray column ( Figure 19 ).

4. With the cable on the bottom, slowly push the tip of the EASY-Spray column into the column holder opening until it clicks into place.

As the EASY-Spray column moves forward into the source, the protective cover over the emitter retracts to expose the emitter tip.

5. Connect the cable to the front output heater socket.

Note Do not pull on the cable from the EASY-Spray column. Use the plug to connect and disconnect the column.

6. Follow the appropriate procedure in “Connecting the LC to the EASY-Spray Column” on page 27 .

7. Do one of the following to set the temperature for the EASY-Spray column:

• Follow the procedure “Connecting the EASY-Spray P-Bus Temp Control Cable” on page 13 .

You can set the column temperature by using either the EASY-nLC 1000 or the

EASY-nLC II instrument touch screen or the Thermo Xcalibur Instrument Setup window. For instructions, refer to the LC instrument manuals.

EASY-Spray Series Ion Source User Guide

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4

Connecting the EASY-Spray Column Plumbing

Using the nanoViper Fitting

• Follow the procedure “Connecting the Controller Power Supply” on page 12 , and use

the temperature control dial on the front of the source.

Table 6 lists the display readings that can appear on the actual temperature display.

Table 6. Readings for the actual temperature display

Display

--

Description

EASY-Spray column is not connected. xx. Actual temperature while heating up xx

BL

Actual temperature, stabilized

Disconnect and reconnect power to the source.

a a

If the display does not change, you must replace the controller PCB. See

“Replacing the Controller

PCB” on page 44

.

Using the nanoViper Fitting

For some of the LC instruments, the plumbing connects to the EASY-Spray column through

several nanoViper fittings, such as the one shown in Figure 20 . Although these fittings

can withstand ultra-high-performance LC (UHPLC) backpressures up to ~1034 bar

(~15 000 psi), they are fingertight fittings, which require only very small torques to seal.

Therefore, you must follow the procedure below to avoid damage by overtightening.

Figure 20. nanoViper fitting

PEEK sealing surface Removable knurled tightening tool

Screw

To use a nanoViper fitting

1. Insert the nanoViper fitting into the target port and slowly rotate the screw clockwise until you feel resistance.

2. Using the black knurled fitting tool, tighten the screw clockwise to an angle of

0–45 degrees (1/8-turn).

3. Start operating the system at the desired working pressure and check the backpressure.

4. If the backpressure is too low, check the system for leaks.

For instructions, refer to the documentation for the LC instrument.

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EASY-Spray Series Ion Source User Guide Thermo Scientific

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Connecting the EASY-Spray Column Plumbing

Connecting the LC to the EASY-Spray Column

5. If the backpressure continues to be too low, return the system to atmospheric pressure.

IMPORTANT To extend the lifetime of the nanoViper fittings, open and close the connections at only atmospheric system pressures. Opening and closing connections at high system pressures can reduce the lifetime of the fittings.

6. Tighten the screw by as much as an additional 45 degrees. Do not turn the screw beyond an angle of 90 degrees from where you felt the initial resistance.

IMPORTANT To prevent damage to the sealing surface of the nanoViper fitting, do not overtighten the fitting.

Connecting the LC to the EASY-Spray Column

This section describes how to connect the one or two plumbing lines from a nanoLC instrument to the EASY-Spray column in either a one- or two-column configuration.

Table 5

on

page 23

lists the required parts.

Note The EASY-nLC Series instruments identify the two solvent lines as “Column Out” and “Waste In.”

CAUTION Wear protective gloves and eye wear when handling the solvent lines.

Follow the appropriate procedure:

Connecting an EASY-nLC 1000 Instrument to the EASY-Spray Column

Connecting an EASY-nLC II Instrument to the EASY-Spray Column , on page 30

Connecting an RSLCnano System to the EASY-Spray Column , on page 33

Connecting an EASY-nLC 1000 Instrument to the EASY-Spray Column

Follow the appropriate procedure for a one- or two-column setup. The two-column setup includes a precolumn connected in the column-out flow path to the venting tee.

Note Remove the nuts and ferrules from the venting tee.

To plumb a one-column setup from an EASY-nLC 1000 instrument

To plumb a two-column setup from an EASY-nLC 1000 instrument , on page 29

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Connecting the EASY-Spray Column Plumbing

Connecting the LC to the EASY-Spray Column

To plumb a one-column setup from an EASY-nLC 1000 instrument

1. On the Column Out line, insert the nanoViper fitting into the venting tee’s side port as shown in

Figure 21

, tighten the fitting by hand until you feel resistance, and then tighten the fitting again by an additional 1/8- to 1/4-turn. Do not tighten the fitting by more than a 1/4-turn.

Figure 21. Recommended plumbing locations on the venting tee (nanoViper example)

Location for the Column Out line

Location for the EASY-Spray column

Location for the Waste In line

CAUTION Because the fittings seal against each other inside the union, do not tighten them by more than 1/4-turn (90 degrees). Overtightening the fittings can irreparably damage their sealing surfaces.

For additional information about nanoViper fittings, see

“Using the nanoViper

Fitting” on page 26 .

2. On the Waste In line, insert the fitting into the venting tee’s bottom port, tighten the fitting by hand until you feel resistance, and then tighten the fitting by an additional

1/8- to 1/4-turn. Do not tighten the fitting by more than a 1/4-turn.

3. Insert the hex key into the bolt that secures the (venting) Tee holder to the column holder, loosen the hex socket-head bolt, and then slowly move the Tee holder forward

( Figure 22

).

Figure 22. Tee holder secured to the bottom of the column holder

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EASY-Spray Series Ion Source User Guide

Tee holder (extended position)

Hex socket-head bolt for the Tee holder

Thermo Scientific

4

Connecting the EASY-Spray Column Plumbing

Connecting the LC to the EASY-Spray Column

4. Connect the venting tee to the EASY-Spray column to complete the one-column setup

( Figure 23

).

Figure 23. Example of a one-column setup with nanoViper fittings

Column Out line

EASY-Spray column

Thermo Scientific

Waste In line

5. Adjust the Tee holder’s position so that it supports the nanoViper fitting, and then use the hex key to tighten the hex socket-head bolt on the bottom of the column holder.

To plumb a two-column setup from an EASY-nLC 1000 instrument

1. Assemble and connect the Column Out line as follows: a. Insert the nanoViper fitting on the Column Out line into the Viper union, tighten the fitting by hand until you feel resistance, and then tighten the fitting again by an additional 1/8- to 1/4-turn (45–90 degrees). Do not tighten the fitting by more than a 1/4-turn.

Note See the Caution on page 28 .

b. Connect the other end of the union to the appropriate end of the nanoViper precolumn.

Note Follow the recommended flow direction stated on the precolumn label. c.

Insert the fitting on the other end of the precolumn into the venting tee’s side port

( Figure 21 on page 28

), and then tighten as noted in

step 1a

.

2. On the Waste In line, insert the fitting into the venting tee’s bottom port, tighten the fitting by hand until you feel resistance, and then tighten the fitting again by an additional 1/8- to 1/4-turn. Do not tighten the fitting by more than a 1/4-turn.

3. Insert the hex key into the bolt that secures the (venting) Tee holder to the column holder, loosen the hex socket-head bolt, and then slowly move the Tee holder forward

( Figure 22 on page 28

).

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Connecting the EASY-Spray Column Plumbing

Connecting the LC to the EASY-Spray Column

4. Connect the venting tee to the EASY-Spray column to complete the two-column setup

( Figure 24

).

Figure 24. Example of a two-column setup with nanoViper fittings and a Viper union

Column Out line EASY-Spray column

Waste In line

5. Adjust the Tee holder’s position so that it supports the nanoViper fitting, and then use the hex key to tighten the hex socket-head bolt on the bottom of the column holder.

Tip When you install the two-column assembly, clear the One Column Setup check box in the Maintenance menu (choose Maintenance > Devices, select the instrument, and then select the Properties tab). For instructions, refer to “Setting Up the Properties for the

HPLC Device” in Chapter 4 of the EASY-nLC Series Getting Started Guide.

When the One Column Setup check box is clear (not selected), valve W on the

EASY-nLC 1000 moves to position 1–6 during the sample loading step. Excess solvent A exits the venting tee and passes through valve W to the waste beaker.

Connecting an EASY-nLC II Instrument to the EASY-Spray Column

Follow the appropriate procedure for a one- or two-column setup. The two-column setup includes a precolumn connected in the column-out flow path to the venting tee.

Note Remove the nuts and ferrules from the venting tee.

To plumb a one-column setup from an EASY-nLC II instrument

To plumb a two-column setup from an EASY-nLC II instrument

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4

Connecting the EASY-Spray Column Plumbing

Connecting the LC to the EASY-Spray Column

To plumb a one-column setup from an EASY-nLC II instrument

1. Attach the nuts and ferrules to the Column Out and Waste In lines as follows: a. Insert one of the lines into a sleeve, and then slowly push it forward until the line is parallel with the end of the sleeve. b. Slide the nut and then the ferrule onto the sleeved line until the sleeve extends past

the ferrule ( Figure 25 ).

c.

Repeat

step 1a and step 1b with the second line.

Figure 25. LC line with sleeve, ferrule, and nut

Nut Ferrule

Sleeve

2. On the Column Out line, insert the fittings into the venting tee’s side port (

Figure 21 on page 28 ), tighten the nut by hand until you feel resistance, and then use the 1/4 in.

wrench to tighten the nut until firmly secured—do not overtighten.

3. On the Waste In line, insert the fittings into the venting tee’s bottom port, tighten the nut by hand until you feel resistance, and then use the 1/4 in. wrench to tighten the nut until firmly secured—do not overtighten.

4. Insert the hex key into the bolt that secures the (venting) Tee holder to the column holder, loosen the hex socket-head bolt, and then slowly move the Tee holder forward

( Figure 22 on page 28

).

5. Connect the venting tee to the EASY-Spray column to complete the one-column setup.

6. Adjust the Tee holder’s position so that it supports the nanoViper fitting, and then use the hex key to tighten the hex socket-head bolt on the bottom of the column holder.

Figure 23 on page 29

shows an example of a one-column setup for the EASY-nLC 1000 instrument, which uses nanoViper fittings.

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4

Connecting the EASY-Spray Column Plumbing

Connecting the LC to the EASY-Spray Column

To plumb a two-column setup from an EASY-nLC II instrument

1. Assemble and connect the Column Out line as follows: a. Remove the smaller fitting from the ZDV adapter union, insert the Column Out line into the fitting, and then slowly push the line forward until it extends past the fitting

( Figure 26

).

Figure 26. Column Out line with smaller fitting from the ZDV adapter union

Smaller fitting (IDEX P/N F-126S) for the adapter union b. With the plug inserted into the ZDV adapter union’s larger end, insert the

Column Out line into the union’s smaller end, and then tighten the smaller fitting until you feel resistance. c.

Remove the plug from the adapter union, and then connect the adapter union’s larger end to the appropriate end of the nanoViper precolumn.

Note Follow the recommended flow direction stated on the precolumn label. d. Insert the fitting at the other end of the precolumn into the venting tee’s side port

( Figure 21 on page 28 ), and then tighten the fitting by an additional 1/8- to 1/4-turn

(45–90 degrees). Do not tighten the fitting by more than a 1/4-turn.

CAUTION Because the fittings seal against each other inside the union, do not tighten them by more than 1/4-turn (90 degrees). Overtightening the fittings can irreparably damage their sealing surfaces.

For additional information about nanoViper fittings, see

“Using the nanoViper

Fitting” on page 26 .

2. Assemble and connect the Waste In line as follows:

a. Attach a nut and ferrule to the Waste In line. See step 1a

and

step 1b in “To plumb a one-column setup from an EASY-nLC II instrument” on page 31 .

b. Insert the nut and ferrule fittings into the venting tee’s bottom port, tighten the nut by hand until you feel resistance, and then use the 1/4 in. wrench to tighten the nut until firmly secured—do not overtighten.

3. Insert the hex key into the bolt that secures the (venting) Tee holder to the column holder, loosen the hex socket-head bolt, and then slowly move the Tee holder forward

( Figure 22 on page 28

).

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Connecting the EASY-Spray Column Plumbing

Connecting the LC to the EASY-Spray Column

4. Connect the venting tee to the EASY-Spray column to complete the two-column setup

( Figure 27

).

Figure 27. Example of a two-column setup for the EASY-nLC II

Column Out line EASY-Spray column

Waste In line

5. Adjust the Tee holder’s position so that it supports the nanoViper fitting, and then use the hex key to tighten the hex socket-head bolt on the bottom of the column holder.

Connecting an RSLCnano System to the EASY-Spray Column

Follow the appropriate procedure for a one- or two-column setup for an RSLCnano system.

The two-column setup includes a precolumn connected across the autosampler valve.

Note To connect the RSLCnano system to the EASY-Spray column, you must use the

Thermo Scientific Dionex UltiMate 3000 RSLCnano EASY-Spray Application Kit

(P/N 6720.0395).

To plumb a one-column setup from an RSLCnano instrument

To plumb a two-column setup from an RSLCnano instrument , on page 35

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4

Connecting the EASY-Spray Column Plumbing

Connecting the LC to the EASY-Spray Column

To plumb a one-column setup from an RSLCnano instrument

1. Insert the nanoViper fitting on the 20 μm ID nanoViper capillary into port 5 of the

autosampler valve ( Figure 28

).

2. Insert the fitting on the other end of the capillary into the Viper union, tighten the fitting by hand until you feel resistance, and then tighten the fitting again by an additional

1/8- to 1/4-turn (45–90 degrees). Do not tighten the fitting by more than a 1/4-turn.

CAUTION Because the fittings seal against each other inside the union, do not tighten them by more than 1/4-turn (90 degrees). Overtightening the fittings can irreparably damage their sealing surfaces.

For additional information about nanoViper fittings, see

“Using the nanoViper

Fitting” on page 26 .

3. Connect the union to the EASY-Spray column to complete the one-column setup

( Figure 28

).

Figure 28. Example of a one-column setup for an RSLCnano system (direct injection)

EASY-Spray column

Viper union

20 μm ID

20 μm ID × 65 cm

Autosampler

1 μL loop

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4

Connecting the EASY-Spray Column Plumbing

Connecting the LC to the EASY-Spray Column

To plumb a two-column setup from an RSLCnano instrument

1. Connect the precolumn to ports 2 and 5 on one of the switching valves (

Figure 29

).

2. Insert the nanoViper fitting on the 20 μm ID nanoViper capillary into port 4 on the same switching valve.

3. Insert the fitting on the other end of the capillary into the Viper union, tighten the fitting by hand until you feel resistance, and then tighten the fitting again by an additional

1/8- to 1/4-turn (45–90 degrees). Do not tighten the fitting by more than a 1/4-turn.

Note See the Caution on page 34 .

4. Connect the union to the EASY-Spray column to complete the two-column setup

( Figure 29

).

Figure 29. Example of a two-column setup for an RSLCnano system (preconcentration)

EASY-Spray column

Viper union

20 μm ID

Precolumn

20 μm ID

Column compartment with two switching valves

EASY-Spray Series Ion Source User Guide

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4

Connecting the EASY-Spray Column Plumbing

Connecting the LC to the EASY-Spray Column

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EASY-Spray Series Ion Source User Guide Thermo Scientific

5

Configuring the Mass Spectrometer for NSI Mode

Follow the applicable procedures to configure the Thermo Scientific mass spectrometer for

nanoelectrospray ionization (nanoESI or NSI)

mode.

Contents

Configuring the EASY-Spray NG Source’s NSI Parameters

Configuring the EASY-Spray Source’s NSI Parameters

Selecting the Source for the LCQ Deca XP Max Mass Spectrometer

Configuring the EASY-Spray NG Source’s NSI Parameters

After you complete the instrument configuration, use the Thermo Tune application to configure the NSI source parameters. For additional information, refer to the Tune Help.

To set the NSI source parameters

1. On the Microsoft™ Windows™ taskbar, choose Start > All Programs >

Thermo Instruments > Thermo model > model Tune, where model is your specific mass spectrometer, to open the Tune window.

2. Click Ion Source, and then click the Ion Source tab to view the Ion Source page

( Figure 30

).

The mass spectrometer automatically detects and enters the source type (NSI) in the

Ion Source Type box.

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5

Configuring the Mass Spectrometer for NSI Mode

Configuring the EASY-Spray Source’s NSI Parameters

Figure 30. Ion Source page in the Tune window for the Orbitrap Fusion (example)

3. In the Pos Ion Spray Voltage (V) box, enter 1900.

Use 1900 V as the start value for the spray voltage. If the intensity of the full-scan spectrum is low, gradually increase the spray voltage to improve the spectrum. The recommended range for the spray voltage is 1400–2400 kV.

4. In the Sweep Gas (Arb) box, enter 0.

5. Click Apply.

Configuring the EASY-Spray Source’s NSI Parameters

After you complete the instrument configuration, use the Thermo Tune Plus application to configure the NSI source parameters. For additional information, refer to the Tune Plus Help.

To set the NSI source parameters

1. Do one of the following to open the Tune Plus window (

Figure 31 ):

• On the Windows taskbar, choose Start > All Programs > Thermo Instruments >

model > model Tune, where model is your specific mass spectrometer.

• In the Thermo Xcalibur™ application, click the Roadmap View icon, the

Instrument Setup icon, the model MS icon, and then Tune Plus.

–or–

• In the Xcalibur application, click the Roadmap View icon, the Instrument Setup icon, and the model MS icon. Then, from the main toolbar choose model >

Start Tune Plus.

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Configuring the Mass Spectrometer for NSI Mode

Configuring the EASY-Spray Source’s NSI Parameters

Figure 31. Tune Plus window for an NSI-configured Velos Pro (example)

Icon for the NSI source

Thermo Scientific

2. Click the NSI Source icon (or choose Setup > NSI Source) to open the NSI Source dialog box (

Figure 32

).

Figure 32. NSI Source dialog box

Enter the absolute value.

3. In the Spray Voltage (kV) box, enter 1.90.

Use 1.90 kV as the start value for the spray voltage. If the intensity of the full-scan spectrum is low, gradually increase the spray voltage to improve the spectrum. The recommended range for the spray voltage is 1.40–2.40 kV.

4. Click OK.

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5

Configuring the Mass Spectrometer for NSI Mode

Selecting the Source for the LCQ Deca XP Max Mass Spectrometer

Selecting the Source for the LCQ Deca XP Max Mass Spectrometer

Note This section is for the LCQ Deca XP Max mass spectrometer, which uses the

Xcalibur data system version 2.0.7 or earlier.

After you install the EASY-Spray source, use the Instrument Configuration window to configure the mass spectrometer for NSI mode.

To configure the mass spectrometer for NSI mode

1. If open, close the Xcalibur data system and Tune Plus application.

2. On the Windows taskbar, choose Start > All Programs > Xcalibur >

Instrument Configuration to open the Instrument Configuration window.

3. Select the devices to control from the Xcalibur data system if not already selected: a. In the Device Types list, select All. b. Under Available Devices, double-click the icons for the mass spectrometer and nanoelectrospray LC instrument to add them to the Configured Devices list.

4. Double-click the mass spectrometer icon to open the Model Configuration dialog box.

5. In the left pane, select Ion Source to display the ion source configuration page, and then select Nanospray in the Default Source list.

6. Click OK, and then click OK again to close the message box.

7. Configure the LC device if you have not done so.

For instructions, refer to the LC instrument documentation.

8. In the Instrument Configuration window, click Done.

9. Restart the data system computer and the mass spectrometer.

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EASY-Spray Series Ion Source User Guide Thermo Scientific

6

Maintenance

This chapter provides maintenance guidelines, a list of required tools and supplies, and instructions for replacing the controller PCB in the EASY-Spray Series source.

Contents

Guidelines

Preparing the Work Area

Tools and Supplies

Cleaning the EASY-Spray Column Emitter Tip

Replacing the Controller PCB

Guidelines

For optimal results, follow these guidelines when performing the procedure in this chapter:

• Take precautions against electrostatic discharge (ESD), especially when the lab environment is at the lower end of the relative humidity specification (see

page xi

). For additional guidelines, refer to the “Operating Environment” chapter in the preinstallation requirements guide for your mass spectrometer.

• Proceed methodically.

• Always place the components on a clean, lint-free surface.

• Never overtighten a screw or use excessive force.

Preparing the Work Area

To prepare the work area

Do the following:

• Cover the area with clean, lint-free paper or a large sheet of clean aluminum foil.

• Have nearby the necessary tools, supplies, and replacement parts (when applicable).

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6

Maintenance

Tools and Supplies

Tools and Supplies

The EASY-Spray Series ion source requires very few tools to perform routine maintenance

procedures. Table 7

lists the necessary tools and supplies for maintaining the ion source and column.

CAUTION Avoid exposure to potentially harmful materials

By law, producers and suppliers of chemical compounds are required to provide their customers with the most current health and safety information in the form of Material

Safety Data Sheets (MSDSs) or Safety Data Sheets (SDSs). The MSDSs and SDSs must be freely available to lab personnel to examine at any time. These data sheets describe the chemicals and summarize information on the hazard and toxicity of specific chemical compounds. They also provide information on the proper handling of compounds, first aid for accidental exposure, and procedures to remedy spills or leaks.

Read the MSDS or SDS for each chemical you use. Store and handle all chemicals in accordance with standard safety procedures. Always wear protective gloves and safety glasses when you use solvents or corrosives. Also, contain waste streams, use proper ventilation, and dispose of all laboratory reagents according to the directions in the MSDS or SDS.

Table 7. Tools, equipment, and chemicals

Description

Tools and equipment

Controller PCB, with a 2.5 mm hex key and a cover plate for use on the EASY-Spray source

EASY-Spray column wash cap

Gloves, lint-free and powder-free

Part number

ES233

ES235

Fisher Scientific 19-120-2947 a

Unity Lab Services :

Graduated cylinder or beaker, glass, 20–60 mm diameter (for use with cleaning solvent)

Sonicator –

• 23827-0008 (size medium)

• 23827-0009 (size large)

Chemicals

Any organic solvent, for example: Fisher Scientific (for example):

• Acetonitrile, Optima™ LC/MS grade

• Methanol, Optima LC/MS grade a

Multiple sizes are available.

• A955-4 (amber glass, 4 L)

• A456-4 (amber glass, 4 L)

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6

Maintenance

Cleaning the EASY-Spray Column Emitter Tip

Cleaning the EASY-Spray Column Emitter Tip

If the video camera’s output shows dust particles on the EASY-Spray column’s emitter tip or if there are unresolved spray issues, follow the procedure in this section to clean the emitter.

CAUTION Wear protective gloves and eye wear when handling solvents.

To clean the EASY-Spray column emitter

1. Carefully remove the EASY-Spray column from the source.

2. Carefully insert the column into the wash cap (

Figure 33 ).

Figure 33. Wash cap installed over the column emitter tip

Wash cap

3. Over a wash container, hold the column and wash cap with one hand, and then slowly pull back on the wash cap to expose the emitter tip.

4. With the other hand, gently wash the emitter tip with a stream of organic solvent.

5. Reinstall the column in the source, and then use the video camera to inspect the emitter tip for any residual lint or particulates.

6. If the emitter tip is not fully clean, use the sonicator as follows: a. With the column inserted into the wash cap, place the emitter tip into the graduated cylinder or beaker. b. Fill the container with the organic solvent to cover the emitter tip. c.

Place the container with the wash cap and column into the sonicator. d. Hold onto these components and run the sonicator for 1 minute.

This completes the cleaning procedure. If you have further issues with the emitter, you might need to replace the column.

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6

Maintenance

Replacing the Controller PCB

Replacing the Controller PCB

If the controller PCB becomes damaged, the actual temperature display might remain off and the EASY-Spray column might not heat. Follow this procedure to install a new controller

PCB.

To replace the controller PCB

1. Follow the procedure “To remove the source from the mass spectrometer” on page 17

.

CAUTION High voltage. To prevent electric shock, make sure that you disconnect the

P-Bus cable before you remove the controller PCB.

2. Using the 2.5 mm hex key, remove the four hex socket-head screws located on the bottom of the source. If you previously installed the controller PCB, P/N ES233, in the

EASY-Spray source, remove the additional cover plate (

Figure 34 ).

Note The cover plate is for use on the EASY-Spray source (ES081) only when installing the ES233 controller PCB.

Figure 34. EASY-Spray with the new controller PCB and extra cover plate (bottom view)

EASY-Spray housing

Controller PCB (ES233)

Cover plate

3. Slowly pull out the enclosure for the controller PCB (

Figure 35 ).

IMPORTANT If you remove the controller PCB too quickly or at an angle, you might damage the PCB components or the internal heater cable routed above the controller

PCB.

Figure 35. Controller PCB (P/N ES233)

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6

Maintenance

Replacing the Controller PCB

4. Unpack the new controller PCB and make sure it is not damaged.

CAUTION To prevent damage to the electronic components on the PCB due to ESD, hold the new controller unit by the metal enclosure instead of the PCB.

5. Slowly push the new controller PCB enclosure into place, and route the heater cable so

that it does not become damaged (pinched) ( Figure 36 ).

Figure 36. Bottom of the EASY-Spray source without the controller PCB

High voltage cable connects to the column holder.

One of the back holes for the hex socket-head screw

6. (EASY-Spray only) Align the screw holes on the additional cover plate over the controller

PCB’s back two screw holes (

Figure 35 on page 44 ).

7. Using the 2.5 mm hex key, install the four hex socket-head screws into the bottom of the source.

You can now install the EASY-Spray Series source back onto the mass spectrometer. To

dispose of the old controller PCB, see “WEEE Directive 2012/19/EU” on page iii .

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6

Maintenance

Replacing the Controller PCB

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EASY-Spray Series Ion Source User Guide Thermo Scientific

7

Troubleshooting

Table 8 lists some EASY-Spray Series ion source and column problems, their causes, and their

possible solutions. You can resolve most problems by using a new EASY-Spray column.

If you need further troubleshooting assistance, contact your local Thermo Fisher Scientific field service engineer. See

“Contacting Us” on page xv

.

Contents

Camera issues

Column issues

Signal issues

Table 8. EASY-Spray Series source problems, causes, and possible solutions (Sheet 1 of 3)

Cause Solution Problem

Camera issues

Camera issues: The camera light is off. Turn on the camera by pressing its top light button

( Figure 18 on page 20 ).

The light is not on.

–or–

The video output is too dark.

Column issues

Heater issues:

The column is not heated.

–or–

The heater is off, or there might be a loose connection or a damaged PCB.

The actual temperature display is blank.

The EASY-Spray column is damaged.

Try these solutions:

• Turn on the heater by selecting a temperature setting.

• Check all of the connections for the controller power supply unit from the electrical wall outlet to the

EASY-Spray Series source.

• See “Replacing the Controller PCB” on page 44

.

Replace the EASY-Spray column.

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7

Troubleshooting

Table 8. EASY-Spray Series source problems, causes, and possible solutions (Sheet 2 of 3)

Problem

Signal issues

The signal intensity is weak.

Cause Solution

The spray is unstable.

–or–

There is no spray.

The parameters for the LC, MS, or both might need adjusting.

Try these solutions:

• Verify that the EASY-Spray column is correctly positioned (that is, clicks on insertion).

• Check the LC method and MS tune method parameters.

The mass spectrometer’s spray cone and ion transfer tube are dirty.

There is a leak somewhere in the liquid path.

Air bubbles in the emitter might cause the spray to “spit.”

• Run a known standard to check the sensitivity.

Clean both the spray cone and ion transfer tube. For instructions, refer to the mass spectrometer’s documentation.

Check all of the LC connections.

Try degassing the mobile phase or purging the line, and then recheck the line for air bubbles.

There is an emitter blockage from particles in the sample, other small particles from the flow lines or valves, and so on.

The EASY-Spray column is out of alignment, which might occur if you bumped the source or moved the source from one MS to another MS.

Try adjusting the spray voltage. If that does not resolve the

blockage problem, either clean the emitter (see

insert a new EASY-Spray column.

page 43

The cleaning solvent is not

LC/MS grade, which can negatively affect the spray stability.

Use an LC/MS-grade solvent to clean the emitter tip.

) or

Follow the procedure “Adjusting the Emitter Tip Position” on page 15

.

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Troubleshooting

Table 8. EASY-Spray Series source problems, causes, and possible solutions (Sheet 3 of 3)

Problem

(continued)

Cause

The source high-voltage (HV) connection might be unstable.

Solution

Check the high voltage contact on the back of the source

( Figure 9 on page 10

and Figure 10 on page 11

) and the front of the mass spectrometer.

If the problem continues for the EASY-Spray NG source, do the following:

1. Open Tune and view the Ion Source page (see

page 38 ).

2. Compare the setting for the spray voltage to the adjacent readback value.

A green box ( ) indicates that the parameter is functioning properly.

The source’s HV connector has a loose connection.

If the problem continues for the EASY-Spray source, do the following:

1. Open Tune Plus, choose View > Display Status View, and then click the All tab.

2. Choose Setup > NSI Source to open the NSI Source dialog box.

3. Compare the setting in the NSI Source dialog box to the readback value for the NSI source’s absolute spray voltage.

A green check mark ( the parameter is functioning properly.

) indicates that

If you have a digital multimeter that can measure 20 M

(mega ohms), do the following:

1. Test the meter’s batteries, and then set the meter to the appropriate resistance setting or range.

2. Measure the resistance from the HV pin on the back of the source to the spring-loaded bolt located inside the

housing ( Figure 9 on page 10 , or

Figure 10 on page 11 ).

A functional HV connection has a measured resistance of 18–22 M,.

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Troubleshooting

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EASY-Spray Series Ion Source User Guide Thermo Scientific

8

Replaceable Parts

This chapter lists the consumable and replaceable parts for the EASY-Spray Series ion sources, and their corresponding part numbers. To order any of these parts, contact your local

Thermo Fisher Scientific field service engineer or go to www.proxeon.com/easyspray .

Contents

Spare Parts

Consumables

Spare Parts

A/B mixing/venting tee . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . SC901

EASY-Spray controller PCB (with a 2.5 mm hex key and cover plate) . . . . . . . . . . . ES233

EASY-Spray controller power supply with power adapter cable . . . . . . . . . . . . . . . . . ES231

EASY-Spray tools: emitter positioning tool and hex keys (1.5 mm and 3 mm) . . . . . ES232

Monitor and power supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ES217

Tee holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ES234

Video camera and power supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ES216

Wash cap (for the EASY-Spray column) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ES235

Consumables

Peptide Columns

EASY-Spray column, 15 cm × 75 μm ID, PepMap™ C18, 3 μm particles,

100 Å pore size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ES800

EASY-Spray column, 15 cm × 50 μm ID, PepMap RSLC C18, 2 μm particles,

100 Å pore size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ES801

EASY-Spray column, 25 cm × 75 μm ID, PepMap RSLC C18, 2 μm particles,

100 Å pore size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ES802

EASY-Spray column, 50 cm × 75 μm ID, PepMap RSLC C18, 2 μm particles,

100 Å pore size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ES803

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8

Replaceable Parts

Consumables

Intact Protein Columns

EASY-Spray column, 25 cm × 200 μm ID, PepSwift™ Monolith . . . . . . . . . . . . . . . ES810

EASY-Spray column, 15 cm × 75 μm ID, Accucore™ C4, 2.6 μm particles,

150 Å pore size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ES811

EASY-Spray column, 15 cm × 75 μm ID, PepMap C18, 3 μm particles,

300 Å pore size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ES812

52

EASY-Spray Series Ion Source User Guide Thermo Scientific

G

Glossary

A

B C

D E

F G

H I

J K L M

N

O P Q R

S

T U V W X Y Z

A

API ion transfer tube A tube assembly that assists in desolvating ions that are produced by the ESI, nanoESI (NSI), or APCI probe (or spray insert).

atmospheric pressure chemical ionization (APCI) A soft ionization technique done in an ion source operating at atmospheric pressure. Electrons from a corona discharge initiate the process by ionizing the mobile phase vapor molecules. A reagent gas forms, which efficiently produces positive and negative ions of the analyte through a complex series of chemical reactions.

atmospheric pressure ionization (API) Ionization performed at atmospheric pressure by using

atmospheric pressure chemical ionization (APCI) , electrospray (ESI) , or

nanoelectrospray ionization

(nanoESI or NSI) .

D

dynamic nanoelectrospray See

nanoESI nanoLC gradient separation .

E

electrospray (ESI) A type of atmospheric pressure ionization that is currently the softest ionization technique available to transform ions in solution into ions in the gas phase.

electrospray ionization (ESI) See electrospray (ESI)

.

H

heated-electrospray (HESI) A type of atmospheric pressure ionization that converts ions in solution into ions in the gas phase by using electrospray (ESI) in combination with heated auxiliary gas.

heated-electrospray ionization (H-ESI) See

heatedelectrospray (HESI) .

I

ion source A device that converts samples to gas-phase ions.

ion sweep cone A removable cone-shaped metal cover that fits on top of the API ion transfer tube and acts as a physical barrier to protect the entrance of the tube.

N

nano liquid chromatography (nanoLC) Liquid chromatography with typical flow rates of

10–1000 nL/min and 10–150 μm diameter columns.

Thermo Scientific EASY-Spray Series Ion Source User Guide

53

Glossary: nanoelectrospray ionization (nanoESI or NSI)

nanoelectrospray ionization (nanoESI or NSI) A type of

electrospray (ESI) that accommodates very

low flow rates of sample and solvent on the order of

1–20 nL/min (for static nanoelectrospray ) or

100–1000 nL/min (for dynamic nanoelectrospray, which is also called

nanoESI nanoLC gradient separation

).

nanoESI nanoLC gradient separation Employs microscale capillary columns to separate the analytes in complex mixtures. The sample is loaded onto a column using an injection valve or a gas pressure vessel. The mixture components are then eluted by a solvent gradient and pumped through the emitter.

nanoESI (NSI) source The entire nanoelectrospray ion source.

nanoESI (NSI) spray current The flow of charged particles in the nanoESI (NSI) source. The voltage on the NSI spray needle supplies the potential required to ionize the particles.

nanoESI (NSI) spray voltage The high voltage that is applied to the spray needle in the nanoESI (NSI) source to produce the NSI spray current as liquid emerges from the nozzle. The NSI spray voltage is selected and set; the NSI spray current varies.

nanospray ionization (NSI) See

nanoelectrospray ionization (nanoESI or NSI) .

S

source See ion source

.

static nanoelectrospray A device that performs continuous analysis of small analyte solution volumes over an extended period of time.

54

EASY-Spray Series Ion Source User Guide Thermo Scientific

I

Index

A

axes control

x- and z-

16

y-

17

C

camera connecting

19

light button and focusing wheel, locations

20

removing

18

troubleshooting

47

cleaning, emitter tip

43

column assembly

25

cleaning

43

installing

24 –

25

part numbers for intact protein

52

peptide

51

setting the temperature

25

consumables

51

contacting us

xv

controller PCB description

4

power supply for

5

replacing

44

D

directive, WEEE

iii

documentation accessing

x

additional

x

downloading

x

E

EASY-Spray column

See column

Thermo Scientific

EASY-Spray P-Bus Temp Control Cable connecting

13

description

5

EASY-Spray Series ion source

See ion source

electrical wall outlets

7

emitter adjusting the tip position

16

tip, cleaning

43

troubleshooting

48

environmental requirements

xi

F

figures, list of

vii

H

heater cable

25

troubleshooting

47

high voltage connector, location

10 –

11

internal cable, location

45

troubleshooting

49

housing

See ion source

I

ion source axes control

x- and z-

16

y-

17

benefits

1

column, installing

25

compatible mass spectrometers

xi

connecting controller power supply unit

12

EASY-Spray P-Bus Temp Control Cable

13

EASY-Spray Series Ion Source User Guide 55

Index: K monitor and camera

19

Tee holder

13

housing description

3

installing

9

kits

xii

locking levers, positions

10

power connection

12

removing

17

ion sweep cone

9

K

kits

EASY-Spray Ion Source

xii

EASY-Spray NG Ion Source

xii

UltiMate 3000 RSLCnano EASY-Spray Application

33

L

LC connections

EASY-nLC 1000, connecting to

27

EASY-nLC II, connecting to

30

RSLCnano system, connecting to

33

tools and supplies

23

locking levers, positions

9

M

maintenance controller PCB, replacing

44

guidelines

41

tools, equipment, and chemicals

42

work area, preparing

41

mass spectrometer diagnostics

49

ion sweep cone

9

NSI mode manually, LCQ Deca XP Max

40

power requirements

xi

preparing for the source

8

setting the NSI source parameters

37

source, removing

17

spray cone

10

monitor connecting

19

video picture, adjusting

21

MSDS

42

N

nanoelectrospray, advantages of

2

nanoESI

See NSI mode

nanoViper fittings, using

26

56

EASY-Spray Series Ion Source User Guide

NSI mode manual configuration, LCQ Deca XP Max

40

setting the spray voltage

39

NSI Source dialog box

39

O

organic solvents

42

P

power connection, location

4

power supply units, connecting camera

19

controller

12

monitor

19

product requirements

xi

R

replaceable parts

51

S

SDS

42

signal intensity, troubleshooting

48

source

See ion source

spray troubleshooting

48

voltage readback value

49

setting the value, EASY-Spray

39

setting the value, EASY-Spray NG

38

spray cone, MS

10

T

Tee holder adjusting position

28

configuring

14

installing

13

shipping configuration

14

temperature control dial, settings

4

display codes

26

display location

4

temperature control cable, connecting

13

tools and supplies, connecting the LC

23

troubleshooting

47

Tune application, opening

37

legacy application, opening

38

Thermo Scientific

V

venting tee, recommended LC connections

28

W

wash cap, installing

43

WEEE directive

iii

X

x, y, and z coordinates, column holder

16

Index: V

Thermo Scientific EASY-Spray Series Ion Source User Guide

57

Index: X

58

EASY-Spray Series Ion Source User Guide Thermo Scientific

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