User manual | En Inverted Research Microscope

Inverted Research Microscope

Inverted Research Microscope TE2000

The new TE2000 series builds on the success of its predecessors elevating inverted microscopy to extraordinary performance levels. With the introduction of TE2000-PFS (Perfect Focus System), the series provides the ultimate live-cell imaging platform for cutting-edge research. PFS guarantees accurate focus for long term observations, and the Noise Terminator assures high

S/N ratio images throughout.

The TE2000-S, a basic model that can be dedicated to specific tasks, comes with two output ports.

The TE2000-U is a universal model that comes standard with four output ports.

The TE2000-E incorporates a highprecision motorized focus and vibration-free motorized optical-path changeover mechanism that facilitates image capture in 3D. The TE2000-E comes with five output ports.

By combining the fully motorized TE2000-E with the Nikon Perfect Focus System (PFS), the

TE2000-PFS guarantees constant and accurate focus, making this latest model perfect for live-cell imaging, including TIRF and long term, dynamic time-lapse observations.

Configured with an epi-fluorescence attachment

Live-cell imaging platform

A powerful motorized microscope with real-time focus correction

Farewell to focus drift!

Focus drift is one of the biggest obstacles in time-lapse observation.

The new TE2000-PFS (Perfect Focus System) automatically detects the surface of the coverslip optically and continually corrects focus to compensate for even the most infinitesimal changes. Focus is maintained during long term observations and stage movement. Moreover, the TE2000-PFS includes all the functions of the acclaimed TE2000-E inverted research microscope.

• You can continue stable, in-focus observations over an extended period of time—perfect for time-lapse recording.

• You can minimize photobleaching, which keeps cells alive longer in fluorescence observation, through an overall reduction in the number of images captured.

• You will never again miss sudden changes in your specimen as PFS instantaneously corrects focus drift or

Z-axis changes resulting from temperature drop when adding reagents.

• You can freely select focus planes throughout the specimen and keep focusing on the selected plane thanks to the Simultaneous Optical Offset feature.

Always in perfect focus

Nikon Original!

When PFS is turned on, the position of the coverslip surface is always detected during observation. The data is continuously fed back to the extremely accurate Z-axis control focusing mechanism thanks to Nikon’s propriety COF (Continuous

Optical Feedback) technology*. Focusing precision of less than

1/3 the focal depth of the objective is maintained.

*Patent pending

•PFS on

Configured with an epi-fluorescence attachment

PFS can also work in brightfield, phase, and DIC observations.

Principle of focus detection

Medium; n: approx 1.35

Glass; n: approx 1.5

Water: n: 1.333

Oil; n: 1.514 or Air; n: 1.0

LED detection light

The coverslip surface* is detected by the LED light emitted through the objective.

*Interface of glass and medium in immersion applications or glass and air in dry applications.

▲

Adding reagent

Correction to focus drift caused by expansion/contraction of the plastic dish when reagents are added

Observation method: Laser TIRF

•PFS off

▲

Adding reagent

Perfect focus to the plane of interest

Nikon Original!

Focus is continuously corrected at any plane of interest throughout the specimen by the Simultaneous Optical Offset feature*. Unlike other systems that have to repeat focusing on the coverslip surface and then the plane of interest in alternate shifts, PFS can maintain constant focus on the plane of interest at millisecond refresh speed. Consequently, you will never again miss rapid events in your specimen because of focus drift.

*Patent pending

Focus is maintained during time-lapse recording

Focus detection with infrared light

PFS uses an LED emitting light in the infrared range and an internal linear CCD detector to detect the precise interface, so it does not intrude on the visible wavelengths used for fluorescence emission. This means you can carry out observation and focus control at the same time, with no influence at all on captured images. Single fluorescent molecules can also be visualized at a high S/N ratio.

Single molecular fluorescence image of YFP label receptor

Observation method: DIC

Notes:

• To prevent reduction in transmittance in the near-infrared range (680nm or higher), Nikon recommends removing the PFS optics from the optical path when PFS is not in use.

• During observation of fluorescent dyes with fluorescence spectrum that reaches the near-infrared range (740nm or higher), fluorescence signals may affect PFS performance.

µ m

Contours of the cell photobleach

0 0.1

0.2

0.3 (s)

Cell: dendrite (part) of a primary dispersion culture cell of a hippocampus

Time-lapse image: being photobleached at after 0.3 s

Observation method: TIRF

Motorized microscope for advanced research

The TE2000-E comes standard with linear encoded motorizedfocus and motorized 5-way light port changeover—perfect for advanced research that requires high resolution image capture in

3D, including confocal microscopy and deconvolution processing.

Streamlined operation from a PC

The microscope can be operated from a

PC using Nikon’s NIS-Elements acquisition and analysis software or other third-party application software.

Greater Z-axis precision

The E model features an integrated Z-axis linear encoded readout of 0.05µm when controlled through a connected computer.

Objective anti-collision mechanism

The nosepiece automatically drops when the objectives are being changed, preventing them from hitting the stage— particularly useful for live cell observations.

TE2000-E configured with confocal system

Auto switching between 5 ports

Five output ports, including a bottom port, are standard and can be easily switched via motorized control.

External fine focusing unit

Fine focusing can be easily controlled anywhere on the desktop with this compact unit.

Remote control unit

All motorized units can be operated easily in a darkroom thanks to the phosphorescent display tags.

Extendible configuration

Flexible extendibility facilitates the most sophisticated research

Nikon’s “stratum structure” enables flexible extendibility

Taking advantage of infinity optics, the TE2000’s stratum structure enables the extension of the distance between the microscope body and objectives by up to 80mm (max.).

This design allows the introduction of equipment such as laser tweezers or extra illumination into the optical path without modifying the microscope body as well as a variety of Nikon’s fluorescence illumination accessories.

Nikon’s “stratum structure” can efficiently provide a perfect system for your desired application. Multimode imaging capability is realized with a single microscope.

Epi-fl attachment

Other attachment

Optional stage risers (70mm) allow the mounting of other equipment such as laser tweezers or a laser unit in addition to an epi-fluorescent attachment without modifying the microscope body.

Epi-fl attachment

Standard position

70mm

Epi-fl attachment

Other attachment

Stage risers

Multimode imaging as shown below is possible.

C1plus or C1si confocal laser scanning microscope system

TIRF (Total Internal Reflection

Fluorescence)/epi-fluorescence system

Laser tweezers

The C1confocal microscope system can be paired with a combination of epi-fluorescence and TIRF units. The confocal microscope system and TIRF unit share one laser light source, and allow you to observe TIRF and confocal images of the same specimen with one microscope. Utilizing the stratum structure, it is possible to add laser tweezers in another stratum or level and provide simultaneous observation with either TIRF, confocal or epi-fluorescence excitation.

Specimen

Coverglass

Objective

(CFI Apo TIRF 60X/1.49 oil)

Top Position

Laser Tweezers

DM (400,

505, 565)

Laser

(1064nm)

DM (IR reflection, visible transmitted)

Bottom Position

TIRF/Epi-fl Attachment

Beam splitter

Fiber

Mercury Lamp

C1 confocal scanning head

Laser

(488nm, 532nm)

Detector

Prism

Eyepiece Tube, Camera Port

Epi-fl illumination

Near-IR-DIC attachment /FRET system

CCTV camera

Utilizing the stratum structure, it is possible to mount an epi-fl illumination system and a near-infrared DIC attachment. The image can be observed under epi-fl illumination simultaneously with viewing of dynamics of living cells with near-IR DIC illumination as the image can be divided into two wavelengths by a dichroic mirror inside the filter turret of the near-IR DIC attachment.

In addition, FRET images can be captured with a spectrophotometer attachment.

Furthermore, simultaneous photometry of multiple fluorescence wavelengths with multiphoton excitation is possible.

Prism

IR Filter

Specimen

Coverglass

Halogen

Lamp

Objective

(CFI Plan Apo VC 60X WI)

Top Position

Epi-fl Illumination System

High-speed

Spectroscopic

Fiber Illuminator

DM (400, 505, 565)

Bottom Position

Near-IR DIC Attachment

DM ( = 780nm reflection, visible transmitted)

Dual-Wavelength

Spectrophotometer

IR CCD Camera for

Edge Detection

Eyepiece Tube, Camera Port

Epi-fluorescence images with high contrast

Noise Terminator mechanism

Responding to an increasing demand for fluorescence observation of higher S/N ratio images, Nikon created a new mechanism, Noise Terminator. The TE2000 incorporates this mechanism in the fluorescence illuminator and filter cubes to totally absorb stray light in the optical path. This significantly reduces noise and dramatically increases fluorescence image contrast.

Lenses

Light absorbing material

Light source

Stray light

Fluorescence filter cube

Nikon’s two process mechanism first removes stray light from the filter cube completely, then absorbs it through the light absorbing material.

Therefore stray light is eliminated thoroughly.

High Quality Fluorescence Filters

Each filter/mirror incorporated in these filters has a very sharp rising edge at the corresponding wavelength. By minimizing the crossover of each signal, Nikon succeeded in significantly reducing the loss of excitation wavelength. As a result these filters can provide high quality epi-fluorescence images. There are three types of filters: CFP,

GFP, and YFP.

Viewed with a high quality filter

Viewed with a conventional filter

High performance DIC prisms

The best balance of high contrast and high resolution

By changing the material structure, Nikon succeeded in significantly improving the functionality of the standard combination of DIC modules and sliders.

The excellent balance of contrast and resolution produces high quality DIC images with no color blur at any magnification.

Depending upon the type of specimen, either a high-contrast or high-resolution combination is selectable.

CFI60 optical system

Clear, aberration-free images at any magnification

The TE2000 utilizes Nikon’s world renowned CFI60 infinity optics, known for crisp and clear images at any magnification, while providing higher NAs and longer working distances. The focal length of the tube lens is ideal at 200mm, which avoids induced aberration even when you introduce phase rings, DIC prisms or dichroic mirrors into the optical path.

New Series of Objectives Created with Nikon’s Accumulated Optical Technologies

CFI Plan Apochromat VC Series

• Chromatic aberrations have been thoroughly correct throughout the view field. Suitable for digital imaging.

• Perfect choice for multi-stained, fluorescence specimens and when using brightfield and DIC techniques.

• Axial chromatic aberration has been corrected up to the violet range (405nm), making these objectives highly effective for confocal applications.

• Excellent brightness throughout the view field.

• The 60X water-immersion type, in particular, features high spectral transmittance, even in the 360nm wavelength range.

CFI Plan Apochromat VC 60X Oil, NA 1.40

CFI Plan Apochromat VC 60X Wl, NA 1.20

CFI Plan Apochromat VC 100X Oil, NA 1.40

CFI Apochromat TIRF Series

CFI Apochromat TIRF 60X Oil, NA 1.49 w/correction collar

CFI Apochromat TIRF 100X Oil, NA 1.49 w/correction collar

• The unprecedentedly high NA of 1.49 enables excitation on an even thinner field to produce high S/N ratio TIRF images.

• The world’s-first temperature-change spherical aberration correction ring is provided for in the objective design. Users can easily counteract the influences to the image quality from temperature-induced changes—from 23ºC (room temperature) to 37ºC (physical temperature)—in the refractive index of the immersion oil.

• The correction ring works perfectly in both DIC and epifluorescence microscopy of minute structures. It is also suitable for the laser tweezer method.

• To be used with a regular coverglass and immersion oil.

Intensity distribution of dot image

23ºC 37ºC before correction

When the temperature changes, aberration occurs.

37ºC after correction

Aberration can be corrected using the correction ring.

Multiport design

Multiport design broadens the range of observation and measurement

The TE2000-PFS and TE2000-E have 5 ports. The TE2000-U has 4 ports.

The TE2000-S has 2 ports. These ports distribute light in the ratios given below. The TE2000 can capture or analyze images with multiple cameras.

Main body

1 2

Port select address

3 4 5

TE2000-PFS Eye 100% Right 80% Bottom Front 80% Left 100%

TE2000-E

TE2000-U

Eye 20% 100%*

Eye 20%

Eye 100% Right 80% (Optional) Front 80% Left 100%

TE2000-S Eye 100%

Eye 20%

Left 80%

Eye 20%*

–

Eye 20%

– –

By changing the optional prism, the light distribution ratio of the shaded portions can be changed as below.

*1 Right: 100 Front: 100 Left: 80 (Eye: 20)

*2 Right: 100 Front: 100 Left: 80 (Eye: 20)

*3 Left: 100

The following portions can also be changed by altering the microscope body.

(Optional at the time of purchase)

*2 Bottom: 100

*3 Right: 100

Intermediate magnification module 1x-1.5x

Magnifications of all ports of the TE2000-PFS, TE2000-E and TE2000-U, including the observation port, can be easily changed without the troublesome adjustment accompanying the change of objectives.

TE2000-U/E/PFS

Enhanced overall rigidity

Sturdy design and thermal stability improve precision, minimize focus deviations

Stability for greater precision

To achieve stability that supports focusing precision, Nikon implemented Computer Assisted Engineering (CAE) and adopted a new high-strength alloy material in the microscope body. This doubled the rigidity compared with previous models.

Improved thermal stability

To minimize focus deviation due to temperature change, Nikon has reinforced the thermal stability of the microscope body, thus improving image quality during long hours of observation or photography.

Glass stage ring

This glass stage ring ensures minimum deformation caused by temperature change, minimizing blurred focus; so it is suitable for time lapse imaging.

User-friendly ergonomic design

Design innovations ensure hours of comfortable, strain-free use

Nikon has instituted numerous innovations to provide comfortable operation and reduce strain, even over a prolonged period of operation.

Nosepiece is inclined to the left for easy handling.

Eye-level riser (as indicated by arrow)

Knobs and buttons

Frequently used buttons and controls are all located at the front and within easy reach.

Fine focusing unit

The TE2000-PFS and TE2000-E come with a compact external fine focusing unit that can be placed anywhere on the desktop.

Nosepiece

The nosepiece is inclined to the left, making it easy to read the magnification and adjust the correction ring.

Stage height

The low-profile stage facilitates handling of specimens.

Main controls are concentrated in the front, close to the operator.

External fine focusing unit

Eyepiece tube

The 25º -inclination eyepiece tube minimizes fatigue during long hours of observation, while its Y-shaped design permits easy viewing of the specimen area on the stage.

Ergonomic tube

An ergonomic tilting eyepiece tube is optionally available.

Furnished with a built-in Bertrand lens, the inclination angle is adjustable from 15º to 45º for viewing in a relaxed and comfortable posture.

Ergonomic tube

Eye-level riser

Optimal eyepiece height can be achieved by using optional eye-level risers. Each riser has a thickness of 25mm and up to two risers can be installed at a time. (The eye-level riser cannot be used with a stage riser)

Large stage-handle knob

Attaching the optional large stage-handle knob enables precise operation for the fine movement of the stage during high magnification observations.

Large stage-handle knob

Retrofittable motorized options

Motorized operation with greater precision and operability facilitates top-notch research

All four models accept retrofittable motorized accessories, allowing researchers to not only choose the desired combinations, but also to control the microscope from external PCs.

Flexible motorized operation for a variety of uses

• A wide variety of motorized accessories are available and can be controlled by an external computer when a HUB controller is attached.

• Motorized switching of objectives and observation methods between epi-fluorescence, Nomarski DIC, phase contrast,

Hoffman Modulation Contrast is possible*.

*There are some restrictions depending on the combination of model and observation method.

Motorized barrier filter wheel

This motorized barrier filter wheel with a turret rotary system can mount up to 8 ø25mm barrier filters.

Motorized system condenser turret

This motorized turret for system condensers enables easier switching between brightfield, phase contrast,

Nomarski DIC, and Hoffman modulation contrast observations.

Motorized sextuple DIC nosepiece

This nosepiece can be used for all observations including DIC. When the magnifications are being changed, the nosepiece automatically descends for easy, safe rotation of the objectives and then returns to the original height after rotation (only in combination with the TE2000-PFS or

TE2000-E model).

Motorized epi-fl filter rotating turret

A maximum of 6 epi-fl filter cubes can be mounted and easily changed with a remote control unit, even in dark rooms.

Communication HUB controller

The HUB controller manages all the cables of each motorized unit at the back of the microscope. By connecting to the RS-232C interface of a PC, motorized units can be controlled from the PC.

Epi-fl attachment with motorized shutter

This epi-fluorescence attachment has a built-in motorized illumination shutter.

Motorized excitation filter wheel

This motorized excitation filter wheel with a turret rotary system can mount up to 8 ø25mm excitation filters.

Motorized analyzer

This motorized analyzer is used to remove and insert an analyzer for

Nomarski DIC.

Remote control unit

7 8

)

(

All motorized units attached to the microscope can be operated from this control pad with an LCD screen. Filter position labels glow in the dark for easy identification.

1. LCD Display

2. Objectives Changeover

3. Shutter Open/Close

4. Excitation Filter Changeover

5. Barrier Filter Changeover

6. Fluorescence Filter Block Changeover

7. Z-axis Reset

8. External Signal Output

9. LCD Operation Mode Changeover

10. LCD Backlight On/Off

11. LCD Brightness Control

12. Diascopic Lamp Control by Remote Pad

13. Diascopic Lamp On/Off

14. Diascopic Lamp Brightness Control

15. Condenser Cassette Changeover

16. Analyzer In/Out

17. Light Path Changing Prism Changeover

Basic Observation Methods

Epi-fluorescence

Flawless, high-contrast fluorescence images

Thanks to a noise terminator mechanism and zoom illumination, the TE2000 achieves an unparalleled

S/N ratio and brightness. It performs well even for weak, single-molecular-level fluorescence observations at leading-edge research.

10.

The Noise Terminator mechanism directs deviated stray light out of the optical path. This results in images of high contrast and unparalleled S/N ratio.

High quality fluorescence filters

Each filter/mirror incorporated in these filters has a very sharp rising edge at the corresponding wavelength that minimizes the crossover of signals.

Zoom-type lamphouse adapter allows the operator to increase light intensity.

Remote control unit and filter turret use phosphorescent display tags to enhance visibility during operation in dark rooms.

Nomarski DIC

With the new DIC system, it is possible to obtain the best image appropriate to the needs

Uniform coloration

Excellent images with uniform coloration are now possible, at any magnifications, by changing the material composition of the DIC prisms.

The perfect balance of high contrast and high resolution

The standard DIC prisms for the system condenser can cover observation at 10X-100X with only two modules that are greatly balanced in contrast and resolution at any magnification. To further closely fit your specific observation needs and specimens, the high contrast DIC prisms and high resolution DIC prisms are also selectable.

High resolution DIC

Observe ultra-minute structures at full optical performance

High NA condensers (Dry, Oil) are available to specifically address the needs for further detailed

DIC images of high magnification observations.

These configurations are optimized for a high resolution video enhanced contrast DIC system.

Ultra-high resolution DIC prisms consisting of a high transmission polarizer and analyzer for each dry and oil type condenser are selectable in addition to standard DIC prisms.

Epi-fluorescence and Nomarski DIC

By combining epi-fluorescence and DIC it is easy to accurately locate fluorescent tagged structures or artifacts within a specimen.

Using Rotating Senarmont Compensation method

Rotate polarizer to change DIC contrast.

Polarizer de Senarmont

Compensator

1/4 Plate

DIC Prism

Slider

11.

12.

Phase contrast

Enables high-contrast imagery of minute living cells

Phase contrast is the most popular observation method for inverted microscopes. This method does not require the staining of the specimen, therefore you can observe and research precise structures of living cells without influencing the live organism.

CFI Plan Fluor ELWD ADL objectives

Nikon developed Apodized phase contrast objectives to effectively reduce halos, which was considered troublesome with the conventional phase contrast method. The internal structures of cells ongoing cell division or thick phase objects used to submerge in halos, making observation difficult, but they are now visible with excellent contrast and a much wider tonal range.

CFI Plan Fluor ADH 100x (Oil) objective

This newly developed high-sensitivity Apodized phase contrast objective dramatically reduces the effect of halo and doubles the contrast of minute objects compared to conventional phase contrast objectives, enabling visualization of minute structures in living tissue and low contrast structures.

Viewed with an ADL objective

13.

ADL objective series

Viewed with an

ADH objective

Viewed with a conventional phase contrast objective

13.

Viewed with a conventional phase contrast objective

ADH 100x (Oil) objective

14.

Hoffman Modulation Contrast®

3D-like images made easy

This technique permits observation of living specimens using plastic petri dishes, which is not possible with DIC. The combination of dedicated

HMC objectives and HMC condenser components creates high contrast 3D-like images of living transparent specimens without the halos seen under phase contrast.

15.

Note: Hoffman Modulation Contrast and HMC are registered trademarks of Modulation Optics, Inc.

Applications

DIGITAL ECLIPSE C1si

A true spectral imaging confocal laser scanning microscope system that can capture spectra across a wide 320nm range with a single scan

• 32-channel simultaneous acquisition suppresses damage to specimens.

• Selectable wavelength resolution from 2.5, 5 or

10nm, independent of pin-hole diameter.

• Acquisition of accurate fluorescence spectra enables color rendering of fluorescence images with greater realism.

• Polarization-enhanced optical sensitivity with DEES improves brightness.

• DISP (Dual Integration Signal Processing) eliminates digitization dead time.

• Spectral imaging via simple switchover from a

3-channel PMT detector.

Overlay of 32-channel images acquired with one shot

Time-lapse recording of 320nm wide spectra

16.

Fluorescence power

(pixel value)

3500

Time

(sec.)

1680

1440

1200

960

720

480

240

3000

2500

2000

1500

1000

500

530 545 560 575 590 605

Fluorescence wavelength (nm)

DIGITAL ECLIPSE C1 plus

The C1 compact, high performance personal type confocal microscope system now supports FRAP

• Simultaneous 3-channel fluorescence, 3-channel plus DIC, time-lapse recording and spatial analysis are possible.

• Filters can be easily exchanged by users to match the latest fluorescent dyes.

• ROI scanning is possible with an optional AOM (Acousto Optical

Modulator)—perfect for FRAP (Fluorescence Recovery After Photobleaching).

• Bi-Directional Scan improves frame rates. Scan Rotation is also possible.

• A greater variety of lasers can be mounted.

Bleached

17.

After 2 sec.

After 30 sec.

After 120 sec.

Laser TIRF-2 system

High signal to noise (S/N) optimizes observation of single molecule activity in fluorescence observation

• A unification of a laser TIRF unit and epi-fluorescence illumination system.

Switching the systems is elementary.

• Responds to various levels of research such as from epi-fluorescence observation of living organisms to observation of living cells at the molecular level.

• Captures single molecule activity in living cells with an extraordinary high

S/N ratio where they contact the coverglass.

• The TE2000’s unique “stratum structure” allows the simultaneous mounting of laser tweezers.

18.

Image under TIRF observation Image under epi-fluorescence observation

TIRF/epi-fluorescence image overlay (pseudo-color)

White light TIRF system

Easily realizes TIRF observation without using laser illumination

• A TIRF function has been provided with the epi-fluorescence attachment. TIRF observation using mercury illumination is available. Xenon and high-intensity halogen can also be used.

• Simply inserting an exclusive aperture (60X, 100X) enables switching to TIRF.

• The wide wavelength band of mercury illumination makes multiple wavelength observation possible by changing the filter. No worries for interference patterns.

Mercury TIRF image Epi-fluorescence image

Photic stimulation unit

Easily realizes photic stimulation without a confocal microscope system

• Compact and easy to attach, easy to operate.

• Observation of molecule movement by photic stimulation in a cell is possible, using fluorescence protein such as Kaede (photo conversion) and PA-GFP

(photo activation).

• Achieves short wavelength correction up to 405nm (h-line). Combined with VC series objectives, in which aberration is corrected to 405nm, it illuminates the targeted area with high precision.

Time-lapse images after stimulation can be recorded in series.

Live cell accessories

NT-88-V3 micromanipulator system

A packaged set of instrumentation required for cellular micromanipulation, the NT-88-V3 is ideal for IVF (in-vitro fertilization), ICSI (intracytoplasmic sperm injection), electrophysiology, or biotechnology applications.

Stage incubation system INU-NI-F1

This all-in-one compact CO

2 incubator sustains the internal temperature at 37ºC with humidity of 90% and CO

2 of 5% to keep the specimen in a stable and precise condition. A special technique that minimizes focus blur facilitates long hours of time-lapse imaging.

Incubator

With an acrylic plastic enclosure providing easy access to the specimen area, this accessory utilizes warm air circulation and maintains the temperature of the interior at 37ºC. The temperature is also adjustable from room temperature to

40ºC.

Thermal plate warmer

A temperature controllable stage ring with a glass heating plate keeps the specimen at a set temperature. Temperature is adjustable from room temperature to 50ºC in 0.1ºC increments.

Digital cameras for microscopes

DXM 1200C

The high-definition cooled color digital camera with a Peltier cooling mechanism captures weak fluorescing images clearly by minimizing background noise.

• Super high resolution images with 12.6-mega output pixels.

• High sensitivity reduces shooting time and avoids photobleaching.

• High 15-fps (maximum) transfer rate ensures smooth live images.

• Easy-to-use control software facilitates large-volume shooting.

Digital Sight Series

The Digital Sight series offers a choice of six camera heads and two control units, enabling an image capturing system to be assembled to suit each use.

High-sensitivity cooled monochrome camera head DS-Qi1

• Superior quantitivity with linearity of >98%.

• High sensitivity equivalent to ISO 800.

• Low noise design with average dark current of

0.7e-/pixel/s.

• High frame rate, 1.5-megapixel cooled monochrome CCD.

High-definition cooled color camera head DS-5Mc

• Cooling mechanism retains CCD at room temperature minus 20°C.

• Reduces heat noise.

• High-definition 5.0-megapixel color CCD.

High-speed cooled monochrome camera head DS-2MBWc

• Cooling mechanism retains CCD at room temperature minus 20°C.

• Reduces heat noise.

• High-frame-rate and high-sensitivity 2.0megapixel monochrome CCD.

High-definition/high-speed color camera head DS-Fi1

• High-definition 5.0-megapixel color CCD.

• High resolution and high frame rate.

• High dynamic range and accurate color reproduction.

• Reduces noise.

Configured with DS-5Mc-U2

High-speed color camera head DS-2Mv

• High frame rate, 2.0-megapixel color CCD.

High-speed monochrome camera head DS-2MBW

• High-frame-rate and high-sensitivity 2.0megapixel monochrome CCD.

PC-use control unit DS-U2

• Compact, space-saving design.

• High-speed image transfer to PC via USB

2.0 connection.

• Versatile image capture, processing, measurement and analysis when coupled with imaging software NIS-Elements.

• Allows control of Nikon motorized microscopes.

Standalone control unit DS-L2

• Large 8.4-in. LCD monitor (XGA).

• Pre-programmed imaging modes for different observation methods.

• Various digital interfaces including USB 2.0

connection.

• Direct print possible.

• Allows control of Nikon motorized microscopes.

Imaging software

NIS-Elements

Advanced solutions for your imaging world

Nikon’s NIS-Elements software provides an integrated solution by delivering automated intelligence to microscopes, cameras, components and peripherals.

It handles multi-dimensional imaging tasks with support for capture, display, peripheral device control, and data management & analysis of images.

The database building feature for handling large numbers of multi-dimensional image files enables efficient and fully documented experiments. The intuitive interface simplifies workflow, and fast image acquisition speeds via direct streaming to RAM allow the recording of rapid biological events. The software also supports numerous image processing capabilities including binary and morphological tools for measurement and analysis routines.

The unified control of the entire imaging system offers significant benefits for cutting-edge research applications such as live cell imaging.

Three distinct packages for specific application requirements are available.

NIS-Elements AR (Advanced Research)—for fully automated acquisition and device control through full 6D

(X, Y, Z, Lambda (wavelength), Time, multipoint) image acquisition and analysis.

NIS-Elements BR (Basic Research)—for acquisition and device control through 4D (such as X, Y, Z, Time and X, Y,

Z, Lambda (wavelength)) acquisition.

NIS-Elements D (Documentation)—for supporting color documentation requirements in bioresearch, clinical and industrial applications, with basic measuring and reporting capabilities.

Real time 2D deconvolution

Data management (Built-in image database)

Allows one-click image acquisition and transfer from a camera to a user defined database.

Report generation

The database supports image and meta-data export to a report generator, enabling users to create report templates and printed or PDF-based reports.

Large image stitching

Ultra-high resolution images can be captured with a motorized stage using sophisticated auto focus.

EDF (Extended Depth of Focus): plug-in

Allows the creation of an all-in-focus image from a series of

Z-axis image stacks providing 3D modeling capability and a multi-dimensional image viewer.

Real time 2D deconvolution: plug-in

Supports live on-the-fly or captured deconvolution of an entire image, or specific regions of interest.

Visit www.nis-elements.com for more detailed information.

Merge channels

CFI60 Objectives

Description

Brightfield

Achromat flat field

Plan Achromat

Fluor

Plan Fluor

CFI Achromat 4X

CFI Achromat 10X

CFI75 LWD 16XW

CFI Achromat LWD 20X

CFI Achromat 40X

CFI Achromat LWD 40XC

CFI Achromat 60X

CFI Achromat 100X oil

CFI Achromat 100X oil, iris

CFI Plan Achromat UW 1X

CFI Plan Achromat UW 2X

CFI Plan Achromat 4X

CFI Plan Achromat 10X

CFI Plan Achromat 20X

CFI Plan Achromat 40X

CFI Plan Achromat 40X NCG

CFI Plan Achromat 50X oil

CFI Plan Achromat 100X oil

CFI Plan Achromat 100X WI

CFI Plan Achromat 100X NCG

CFI Fluor 10X W

CFI Fluor 20X W

CFI Fluor 40X W

CFI Fluor 60X W

CFI Plan Fluor 4X

CFI Plan Fluor 10X

CFI Plan Fluor 10XW

CFI Plan Fluor 20X

CFI Plan Fluor ELWD 20XC

CFI Plan Fluor 20X MI

Apochromat

Plan Apochromat

Plan Apochromat VC

S Fluor

CFI Plan Fluor 40X

CFI Plan Fluor 40X oil

CFI Plan Fluor ELWD 40XC

CFI Plan Fluor 60XC

CFI Plan Fluor 60X oil, iris

CFI Plan Fluor ELWD 60XC

CFI Plan Fluor 100X dry

CFI Plan Fluor 100X oil

CFI Plan Fluor 100X oil, iris

CFI Apo 40XW NIR

CFI Apo 60XW NIR

CFI Apo TIRF 60X oil

CFI Apo TIRF 100X oil

CFI Plan Apochromat 2X

CFI Plan Apochromat 4X

CFI Plan Apochromat 10X

CFI Plan Apochromat 20X

CFI Plan Apochromat 40XC

CFI Plan Apochromat 40X oil

CFI Plan Apochromat 60XC

CFI Plan Apochromat 100X NCG oil

CFI Plan Apochromat VC 60X oil

CFI Plan Apochromat VC 60X WI

CFI Plan Apochromat VC 100X oil

CFI S Fluor 4X

CFI S Fluor 10X

CFI S Fluor 20X

CFI S Fluor 40XC

CFI S Fluor 40X oil

CFI S Fluor 100X oil, iris

NA W.D. (mm)

0.8

1.00

1.49

0.10

0.20

0.45

0.75

0.95

1.00

0.95

0.75

1.30

0.60

0.85

0.5-1.25

0.70

0.90

1.30

0.5-1.3

1.40

1.20

1.40

0.20

0.50

0.75

0.90

1.30

0.5-1.30

0.30

0.50

0.80

1.00

0.13

0.30

0.3

0.50

0.45

0.65

0.90

1.25

1.10

0.90

0.10

0.25

0.8 (at 16X)

0.40

0.04

0.06

0.10

0.25

0.40

0.65

0.55

0.80

1.25

0.5-1.25

0.75

0.48

0.35

0.17

2.5

0.26

2.0

17.1

16.0

3.5

2.1

8.1-7.0

Oil 0.35;

Glycerin 0.34;

Water 0.33

0.66

0.2

3.7-2.7

30.0

7.0

3.0

3.8

3.2

7.5

30.0

10.5

1.3

0.57

0.65

2.7-1.7

0.3

0.23

0.17

0.13

0.27

0.13

15.5

1.2

1.0

0.3

0.22

0.2

20.0

4.0

1.0

0.14

0.16

0.15

3.5

2.8

0.13

0.12

8.5

0.3

0.22

2.1-1.5

0.3

0.16

Remarks

Water dipping

Spring loaded

Water dipping

Spring loaded

C.C.0.5-1.5

Spring loaded

Water dipping

Spring loaded

C.C.0-2 with iris

No cover glass with temperature correction ring

No cover glass

C.C.0-2

Multi-immersion; Oil-glycerin-water

Stopper

C.C.0-2

C.C.0.11-0.23

with iris

C.C.0.14-0.2

Stopper with iris

C.C. 0.13-0.22

C.C. 0.13-0.20

C.C.0.11-0.23

Stopper

C.C.0.11-0.23

Stopper

CC.0.15-0.18; Water-immersion

Stopper

C.C.0.11-0.23

Stopper

Description

Phase Contrast

Achromat flat field

Plan Achromat

Fluor

Plan Fluor

Plan Apochromat

S Fluor

Hoffman Modulation Contrast

CFI HMC 10X

CFI HMC LWD 20XF

CFI HMC LWD 40XC

Polarizing

CFI Achromat DL 10X

CFI Achromat ADL 10X

CFI Achromat LWD DL 20X

CFI Achromat LWD DL 20XF

CFI Achromat LWD ADL 20XF

CFI Achromat DL 40X

CFI Achromat LWD DL 40XC

CFI Achromat LWD ADL 40XF

CFI Achromat LWD ADL 40XC

CFI Achromat DL 100X oil

CFI Achromat BM 10x

CFI Plan Achromat DL 10X

CFI Plan Achromat DL 20X

CFI Plan Achromat DL 40X

CFI Plan Achromat DL 100X oil

CFI Fluor DLL 40XW

CFI Plan Fluor DL 4X

CFI Plan Fluor DLL 10X

CFI Plan Fluor DL 10X

CFI Plan Fluor DLL 20X

CFI Plan Fluor ELWD DM 20XC

CFI Plan Fluor ELWD ADL 20XC

CFI Plan Fluor DLL 40X

CFI Plan Fluor ELWD DM 40XC

CFI Plan Fluor ELWD ADL 40XC

CFI Plan Fluor ELWD DLL 60XC

CFI Plan Fluor DLL 100X oil

CFI Plan Fluor ADH 100X oil

CFI Plan Apochromat DM20X

CFI Plan Apochromat DM40XC

CFI Plan Apochromat DM40X oil

CFI Plan Apochromat DM60XC

CFI Plan Apochromat DM60X oil

CFI Plan Apochromat DM100X oil

CFI S Fluor DL 20X

CFI S Fluor DL 40X

CFI Achromat P 4X

CFI Achromat P 10X

CFI Achromat LWD P 20X

CFI Achromat P 40X

CFI Achromat P 100X Oil

0.25

0.40

0.55

0.10

0.25

0.40

0.65

1.25

0.45

0.75

0.60

0.70

1.30

0.65

1.25

0.80

0.13

0.30

0.50

1.30

0.75

0.95

1.0

0.95

1.40

0.75

0.90

0.25

0.40

0.65

0.55

1.25

0.25

0.40

W.D. (mm) Remarks

6.1

3.0

2.7-1.7

30.00

7.00

3.80

0.65

0.23

0.57

0.17

2.0

16.4

16.0

15.2

2.1

8.1-7.0

0.66

3.7-2.7

2.1-1.5

0.16

0.2

1.0

0.14

0.16

0.15

0.21

0.13

1.00

0.30

7.0

6.2

3.8

3.0

0.65

2.7-1.7

2.1

2.7-1.7

0.23

6.10

10.5

1.3

Spring loaded

Water dipping

Spring loaded

CG : Cover Glass thickness (mm)

CC : Correction Collar (mm)

Condensers

Type

T-CT-E Motorized System

Condenser/System Condenser

High NA Condenser

TE-C ELWD Condenser

TE-C SLWD Condenser

TE-C HMC Condenser

ELWD condenser lens

LWD condenser lens

HMC condenser lens

Dry top lens

Water immersion top lens

Oil immersion top lens

0.3

0.52

0.4

0.85

0.9

1.4

0.3

0.12

0.4

W.D. (mm) Ph module

75 L.1.2

L.1.2.3

1.92

190

L.1.2.3

L.1

HMC module DIC module

MC1.MC2.MC3

LWD N1, LWD N2, LWD NR

MC1.MC2.MC3

HNA N2, HNA NR

Magnifications

2-60X

4-100X

10-40X

10-100X

2-20X

4-40X

10-40X

CG 1.2

C.C.0-2

CG 1.2

C.C.0-2

CG 1.2

C.C.0-2

C.C.0.5-1.5

Stopper

C.C.0.11-0.23

Stopper

C.C.0.11-0.23

Stopper

C.C.0.11-0.23

Ph1

Ph2

Ph3

Ph2

Ph3

Ph2

PhL

Ph1

Ph3

Ph2

Ph3

Ph2

Ph3

Ph2

Phase ring

Ph1

Ph2

Ph1

Ph2

Ph3

Ph1

CG 1.2

C.C.0-2

Epi-fluorescence Filters

Filter Characteristics

Filters

UV-1A

UV-2A

UV-2B

UV-2E/C

(DAPI)

V-2A

BV-1A

BV-2A

B-1A

B-1E

B-2A

B-2E/C

(FITC)

B-3A

G-1B

G-2A

G-2B

G-2E/C

(TRITC)

Y-2E/C

(Texas Red)

Wavelengths

EX 365/10

DM 400

BA 400

EX 330-380

DM 400

BA 420

EX 330-380

DM 400

BA 435

EX 340-380

DM 400

BA 435-485

EX 380-420

DM 430

BA 450

EX 435/10

EM 455

BA 470

EX 400-440

DM 455

BA 470

EX 470-490

DM 505

BA 520

EX 470-490

DM 505

BA 520-560

EX 450-490

DM 505

BA 520

EX 465-495

DM 505

BA 515-555

EX 420-490

DM 505

BA 520

EX 546/10

DM 575

BA 590

EX 510-560

DM 575

BA 590

EX 510-560

DM 575

BA 610

EX 540/25

DM 565

BA 605/55

EX 540-580

DM 595

BA 600-660

Characteristics

•Narrow band pass – only 365nm (i line) of Mercury spectrum used

•Narrow band pass minimizes auto-fluorescence and photo-bleaching

•Standard filter block for UV

•Darker background than UV-2A

•For DAPI, cutting off FITC (green) and TRITC (red)

•Soft-coated type for high signal/noise

•Band-Pass Barrier Filter used to cut off green and red

•Standard filter block for V

•Narrow band pass – only 435nm (g line) of Mercury spectrum used

•Narrow band pass minimizes auto-fluorescence and photo-bleaching

•Standard filter block for BV

•Narrower excitation range than B-2A

•FITC + Counter-stain (TRITC, PI)

•For FITC (green), cutting off Rhodamine red

•Band-Pass Barrier Filter used to cut off red

•Standard filter block for B

•For FITC + Counter-stain (TRITC, PI)

•Soft coated type for high signal/noise

•For FITC (green), cutting off Rhodamine red

•Band-pass Barrier Filter used to cut off red

•Wide band pass – recommended for halogen illumination only

•Narrow band pass – only 546nm (e line) of Mercury spectrum used

•Narrow band pass minimizes auto-fluorescence and photo-bleaching

•Standard filter block for G

•610nm barrier provides darker background and deep red emission

•For TRITC (Rhodamine)

•Soft coated type for high signal/noise

•Band-Pass Barrier Filter used to cut off reds above 643nm

•For Texas Red ®

•Soft coated type for high signal/noise

•Band-Pass Barrier Filter used to cut off reds above 660nm

Multi-Band Filters

Filters

Dual

Abbreviations

F-R

F-T

D-F

Applications

FITC

Rhodamine

FITC

Texas Red

DAPI

FITC

Filters for Fluorescent Protein

Models

GFP-L

GFP-B

Wavelengths

EX480/40, DM505, BA510

EX480/40, DM505, BA535/50

Characteristics

GFP long-pass type

GFP band-pass type

Triple

Filters

Applications

GFP

Applications

•DAPI

•Hoechst 33258/33342

•AMCA

•Cascade Blue ®

•Autofluorescence

•Catecholamine

•Serotonin

•Tetracycline

•Quinacrine

•Quinacrine Mustard (QM)

•Thioflavine S

•Acriflavine

•FITC

•Acridine Orange

•Auramine O

•Coriphosphine O

•Bodipy ®

•Fluo-3

•DIO

•TRITC

•Rhodamine B200

•Propidium iodide

•R-Phycoerythrin

•B-Phycoerythrin

•Dil

•Ethidium Bromide

•Texas Red ®

Abbreviations

D-F-R

D-F-T

Applications

DAPI

FITC

Rhodamine

DAPI

FITC

Texas Red

High Quality Filters

Each filter/mirror has a very sharp rising edge at the corresponding wavelength, minimizing signal crossover.

Filters

CFP HQ

GFP HQ

YFP HQ

Wavelengths

EX420-445, DM450, BA460-510

EX455-485, DM495, BA500-545

EX490-500, DM510, BA520-560

Specifications

Main body

Optical system

CFI60 infinity optical system, parfocal distance 60mm

Light distribution

5 positions, motorized light-distribution changer

Observation 100, Left port 100, Right port 80, Front port 80, Bottom port

100

* 3 other models are available as options:

1) left 80/100 switchable instead of bottom 100

2) right 80/100 switchable instead of bottom 100

3) front 80/100 switchable instead of bottom 100

4 positions

Observation 100, Left port 100,

Right port 80, Front port 80

* 4 other models are available as options for 5-position light distribution:

1) with bottom port (must be added,

100% light)

2) left 80/100 switchable

3) right 80/100 switchable

4) front 80/100 switchable

2 positions

Observation 100, Left port 80

* 2 other models are available as options:

1) left 100 instead of 80

2) right port (must be added) 100 instead of left 80

PFS

Compatible specimen

Compatible dish

Detect boundary

Specimens in aqueous solutions

(cultured specimens, in-vitro assays, etc.)

Glass-bottom dishes (thickness: 150-

180µm, No. 1S recommended)

Offset distance Adjustable with the controller dial

Memory function

Offset distance, store/recall of nosepiece up/down distance (up to 6 points per nosepiece)

Focusing

Immersion lens: between glass and medium; between dry lens: glass and air

——

Via motorized/manual nosepiece up/down movement

Stroke—manual: up 7mm, down 3mm; motorized: up 6mm, down 2.5mm

Coarse stroke: 4.9mm/rotation; Fine stroke: 0.1mm/rotation (motorized)

Minimum fine reading: 0.05µm by optical linear encoder

External fine focusing unit

Via nosepiece up/down movement

Stroke—manual: up 7mm, down

3mm

Coarse stroke: 4.9mm/rotation; Fine stroke: 0.1mm/rotation

Minimum fine reading: 1µm

Refocusing stopper: Adjustable coarse torque stopper

Intermediate magnification

Extendible structure

Other

Eyepiece tube

1.5X

Available

Light intensity control; Light on/off switch

(1) T-TD Binocular Tube D

(2) T-TS Binocular Tube S

(3) T-TERG Binocular Ergonomic Tube D

(4) T-TI Intermediate Tube for Eclipse E600/400 trinocular tubes and teaching heads

Eyepiece lens

(F.O.V.)

Illumination

Condenser

CFI 10X (22mm), CFI 12.5X (16mm), CFI 15X (14.5mm)

Nosepiece

Objectives

Stage

Via nosepiece up/down movement

Stroke—manual: up 7mm, down

3mm

Coarse stroke: 4.9mm/rotation; Fine stroke: 0.1mm/rotation

Minimum fine reading: 1µm

Adjustable coarse torque stopper

——

(1) T-DH 100W Illumination Pillar (2) T-DS 30W Illumination Pillar

SLWD condenser for phase contrast*, ELWD condenser for phase contrast*, System condenser LWD**, ELWD** Motorized system condenser

LWD**, ELWD**, Hoffman Modulation condenser® (HMC), High NA condensers

*Only for 30W pillar **Only for 100W pillar

T-N6 Sextuple Nosepiece, T-ND6 Sextuple DIC Nosepiece, T-ND6-E Motorized Sextuple DIC Nosepiece

CFI60 objectives

(PFS function does not work with some objectives.)

CFI60 objectives

(1) T-SR Rectangular Stage—Cross travel: 70 x 50mm; Size: 300 x 276mm

Motorized functions

Epi-fluorescence attachment

Nomarski DIC system

(2) T-SP Plain Stage—Size: 300 x 210mm; Mechanical stage mountable

(3) T-SAM Attachable Mechanical Stage (must be used with T-SP Plain Stage)—Cross travel: 126 x 84mm; Specimen holders attachable

Fine focusing (minimum reading: 0.05µm), Objective anti-collision mechanism (when the nosepiece is rotated), Light distribution changer

—— ——

(Motorized options):

DIC nosepiece, Analyzer, Epi-fl filter rotating turret, Epi-fl shutter, Excitation filter wheel, Barrier filter wheel, System condenser turret

Six fluorescence filter blocks in rotating turret with shutter, Noise Terminator mechanism incorporated, Aperture diaphragm centerable, Field diaphragm centerable, 33mm ND4/ND8 filters, 25mm heat absorbing filter, Lamphouse adapter, Zoom lamphouse adapter (option)

Contrast control: Senarmont method (by rotating polarizer)

Objective side prism: for individual objectives (installed in nosepiece)

Condenser side prism: LWD N1/N2, HNA N2/NR types

*Nomarski DIC system can be attached only to 100W pillar.

Optional accessories

Nikon digital SLR cameras, Digital cameras DXM1200C/Digital Sight series/DQC-FS, Photomicrographic equipment H-III, Teaching head, Drawing tube, CCTV adapters, Micromanipulators, etc.

Power consumption

(max.)

Weight (approx.)

(TE2-PS 100W Power Supply) 100-230V, 2.4A/160W (TE-PS 30W Power Supply) 100/120V, 0.6A/80W; 230V, 0.4A/80W

Phase contrast set: 45kg; Epi-fl set: Phase contrast set: 40kg; Epi-fl set:

50kg 45kg (w/100W pillar)

Phase contrast set: 36kg; Epi-fl set:

41kg (w/100W pillar)

Phase contrast set: 32kg

(w/30W pillar)

System diagram

HMC MC1, MC2,

MC3 Modules

HMC Lens

Eyepieces

CFI 10X,

12.5X, 15X

Centering

Telescope

Y-TT2 Trinocular Tube T2UW

Y-TF2 Trinocular Tube F2UW

Y-IDT Drawing Tube

Teaching Heads

Eyepiece

Tubes

Phase PH-L, PH-1,

PH-2 Modules

ELWD Lens

System Condenser

Turret

Phase PH-L, PH-1,

PH-2, PH-3;

DIC LWD N1,

LWD N2;

HMC MC1,

MC2, MC3 Modules

LWD Lens

T-CT-E Motorized

System Condenser

Turret* 2

T-TD

Binocular Tube D

T-TS

Binocular Tube S

T-TERG

Binocular

Ergonomic Tube D

T-TI

Intermediate

Tube

Eye-level

Riser

Condensers

DIC HNA-N2,

Modules

35mm Petri

Dish Holder

Stage Ring

C-HSG Slide

Glass Holder

Glass

Stage Ring

Hemacytometer

Holder

C-HU Universal

Holder

T-SAM

Attachable Mechanical

Stage

Acrylic

Stage Ring

Stages

T-SSR

Short

Rectangular

Stage

T-SR

Rectangular

Stage

T-SP Plain Stage

DIC HNA-N2,

HNA-NR

Modules

High NA Lens

(Dry)

High NA Lens

(Oil)

High NA

Condenser

Holder

High NA

Condenser

Lens Unit

CFI Objectives

TE-C ELWD

Condenser

DIC Slider

T-N6 Sextuple

Nosepiece

T-ND6 Sextuple

DIC Nosepiece

T-ND6-E Motorized

Sextuple DIC Nosepiece* 2

Nosepieces

C-Mount

Adapter A

C-Mount TV

Adapter VM4X

Side Port

C-Mount

CCTV

Camera

C-Mount TV

Adapter VM2.5X

C-0.7X DXM Relay Lens/

C-Mount Adapter 0.55X

C-Mount

Adapter

0.45X, 0.6X

C-Mount

CCTV

Adapter

Relay

Lens 1X

C-Mount

Zoom

Adapter

Zooming

Lens

Multi-image

Module

T-FLBW-E Motorized

Barrier Filter Wheel* 2

T-BPA

Photo Adapter

35mm SLR

Camera

T-BSLR

Front-port

SLR Adapter

High-definition

Digital Camera

DXM1200

C-mount TV

Camera T-BDCA

Front-port

Direct C

Adapter

T-PFS

Perfect

Focus Unit

T-FLC-E

Motorized

Epi-fl Filter

Rotating Turret

Eyepiece

Tubes

Stages Nosepieces

Stage Riser

* 3

Epi-fl

Attachments for PFS

8’

Illumination

Pillars

Front Port

Analyzer

T-HUBC

Communications

Hub Controller

T-PFS Perfect Focus

Controller

REROCUS

COARSE FOCUS

ESCAPE

Photomicrographic

System FX-III Series

Bottom Port 4 Side Port

T-EFN Focus Knob

Nikon

Digital SLR

Camera

ENG-Mount

CCTV Camera

Adapters

D-SLR F-mount

Adapter

* 3 Essential for PFS.

T-RCP

Remote

Control Pad

T-AC

AC Adapter

Lambda Plate

T-P Polarizer

Halogen Lamp

12V-100W

Filter 45mm;

GIF, NCB11, ND16, ND2

Heat Absorbing

HMX

Lamphouse

Adapter

Collector

Lens

Halogen Socket

100W

SLWD Condenser

Filter 33mm;

GIF, Heat Absorbing,

NCB11, ND16, ND2

HMX

Lamphouse

Halogen Lamp

6V-30W

Epi-fl

Attachments

Condensers

T-DH

100W Illumination Pillar

T-FLC Epi-fl Filter Rotating Turret

Epi-fl

Filter

Blocks

T-FL

Epi-fl

Attachment

Halogen Lamp

12V-100W LL

Adapter

* 1

Lamphouse-2 BL

Remote Cable

TE2-PS

12V Power Supply for

100W Illumination Pillars

HMC MC1, MC2,

MC3 Modules

System Condenser

Turret

HMC Lens

TE-C ELWD

Condenser

Illumination Pillars

T-FLA

FL Illumination

Adapter

T-FLEW-E

Motorized Excitation

Filter Wheel* 2

TE-AT

Double

Lamphouse

Adapter

C-FC

Epi-fl

Collector

Lens

T-DS

30W Illumination Pillar

Remote Cable

Lamphouse

HMX-3B/HMX4B w/Backmirror

Mercury Lamp

Socket S 100W

TE-PS

6V Power Supply for 30W Illumination Pillar

* 1 Provided with the HMC lens.

Mercury

Lamp

HG-100W

Starter

HG-100W

T-FLZA

FL Zoom

Illumination

Adapter

Epi-fl

Collector

Lens Q2

Xenon

Starter

Xenon

Lamp

T-FLC-E Motorized Epi-fl Filter

Rotating Turret*

Epi-fl Attachments for PFS

8’

T-FL-E Epi-fl

Attachment with

Motorized

Shutter* 2

Xenon

Lamphouse

Xenon

Lamp Socket

Halogen

Lamp

12V-100W

Stage Riser

T-BSUK70

Stage Riser

T-BSUK70

Stage Riser

Analyzer

T-A Analyzer

T-A-E Motorized

Analyzer* 2

HMX

Lamphouse

U Transformer

Halogen Socket

100W

Front Port

Eyepiece

Tubes

Stages

Nosepieces

Illumination

Pillars

Stage Riser

Epi-fl

Attachments

Analyzer

T-HUBC

Communications

Hub Controller

Front Port

Eyepiece

Tubes

Stages

Nosepieces

Illumination

Pillars

6 7

Stage Riser

Epi-fl

Attachments

Analyzer

T-HUBC

Communications

Hub Controller

Eyepiece

Tubes

Stages

Nosepieces

Illumination

Pillars

Stage Riser

Epi-fl

Attachments

Analyzer

T-HUBC

Communications

Hub Controller

REROCUS

COARSE FOCUS

ESCAPE

Bottom Port 4 Side Port

T-AC

AC Adapter

T-EFN Focus Knob

T-RCP

Remote

Control Pad

Model with bottom port also available

Side Port

T-AC

AC Adapter

T-RCP

Remote

Control Pad

Side Port

(left side)

T-AC

AC Adapter

T-RCP

Remote

Control Pad

Requires a communications hub controller.

Dimensional diagram

Unit: mm (inch)

144.5 (5.7)

Eyepoint

163.5 (6.4)

195 (7.7)

260 (10.2)

144.5 (5.7)

19.6 (0.8)

476.4 (18.8)

618 (24.3)

Eyepoint

163.5 (6.4)

144.5 (5.7)

Eyepoint

163.5 (6.4)

195 (7.7)

260 (10.2)

144.5 (5.7)

41.3 (1.6)

COARSE FOCUS

ESCAPE

476.4 (18.8)

598 (23.5)

Eyepoint

154.6 (6.1)

195 (7.7)

260 (10.2)

41.3 (1.6) 135.4 (5.3)

476.4 (18.8)

Photos courtesy of:

1 JC1 stained (Mitochondrial membrane potential) Murine 2 cell embryo, In-vivo derived—

David Froiland Bsc., Deidre Zander Bsc. Hons., Michelle Lane PhD., Research Centre for

Reproductive Health, University of Adelaide, Australia

2 Albino Swiss mouse embryo fibroblast cell

6, 9, 10, 16, 17

Michael W. Davidson, National High Magnetic Field Laboratory, USA

Aplysia bag cell neuronal growth cone—Dylan Burnette, Paul Forscher Laboratory, Yale University, USA

Profs. Akihiro Kusumi and Dr. Chieko Nakada, Kusumi Laboratory, Institute for Frontier Medical

Sciences, Kyoto University

Dr. Chieko Nakada, Kusumi Office, Institute for Frontier Medical Sciences, Kyoto University

Co-researcher: Professor Shigeo Okabe, Tokyo Medical and Dental University.

CoxIV-Venus expressed in mitochondria—Takeharu Nagai, Ph.D., Professor, Laboratory for

Nanosystems Physiology, Research Institute for Electronic Science, Hokkaido University

Enter the “Microscopy University” on the web and discover a whole new world.

www. microscopyu.com

WARNING

TO ENSURE CORRECT USAGE, READ THE CORRESPONDING

MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT.

* Monitor images are simulated.

Company names and product names appearing in this brochure are their registered trademarks or trademarks.

195 (7.7)

260 (10.2)

135.4 (5.3)

476.4 (18.8)

Neut lung cells in culture—Alexey Khodjakov, PhD., Wadsworth Center, NY Dept. of Health, USA

Sea urchin egg—Yukihisa Hamaguchi, PhD, Tokyo Institute of Technology, Japan

Rat embryo in cell division (EGFP transgenic rat)—Masumi Hirabayashi, PhD, YS New Technology

Inst. Inc., Japan

Monkey kidney cells—Satoko Takaoka, Utsunomiya East Hospital, Japan

NG108-15 cell—Dr. Kaoru Kato, Neuroscience Research Institute, The National Institute of

Advanced Industrial Science and Technology (AIST)

Bovine ovum—YS New Technology Inst. Inc., Japan

JC-1 loaded nerve cells—Dr. Yasushi Okada, Cell Biology, Medical Dept of Graduate School, Tokyo

University

Hela cell nucleus expressing H1 Histon-GFP—Dr. Hiroshi Kimura, Horizontal Medical Research

Organization, Kyoto University Faculty of Medicine

Fixed 3T3 fibroblasts—Dr. Gregg G. Gundersen, Columbia University, USA 18

Please contact Nikon for a handy pamphlet listing compatible accessories, including objectives and epi-fluorescence filters.Some models are not available in certain areas. Please check with your local Nikon representative for details.

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