innovation since 1796 Polarimeter P3000

innovation since 1796 Polarimeter P3000
Polarimeter
P3000
innovation since 1796
User Manual
www.kruess.com
Table of Contents
Chapter - 1
Introduction ................................................................................... 5
1.1 Application Examples ..................................................................... 5
1.2 Reading Information ....................................................................... 6
Chapter - 2 General Safety Instructions ............................................................ 7
Chapter - 3 Instrument Description................................................................... 9
3.1 Scope of Delivery ............................................................................ 9
3.2 Optional Accessories ...................................................................... 9
3.3 Required Reagents ......................................................................... 9
Calibration .................................................................................. 9
Accuracy Check ........................................................................... 9
Sample Dilution ........................................................................... 9
3.4 Instrument Images ........................................................................10
Instrument Front View of P3000 .................................................. 10
Instrument Back View of P3000 ................................................... 10
Polarimeter Tubes ...................................................................... 10
3.5 Screen Descriptions ...................................................................... 11
Main Menu ............................................................................... 11
Explanation of Buttons ................................................................ 11
Explanation of Display Window ................................................... 12
3.6 Measurement Principle.................................................................. 13
Chapter - 4 Basic Operation............................................................................. 14
4.1 Buttons ......................................................................................... 14
4.2 Defining the System Settings ........................................................16
4.3 Defining the Instrument Settings................................................... 17
4.4 Performing the Calibrations.......................................................... 18
Chapter - 5
Initial Startup ................................................................................19
5.1 Installation ....................................................................................19
Choosing a Suitable Location ..................................................... 19
Connecting a Printer .................................................................. 19
1
Connecting the Polarimeter to the Power Supply ........................... 19
5.2 Preparing the Instrument after Installation ................................... 20
Switching on the Instrument ........................................................ 20
Switching on the Printer .............................................................. 20
Connecting the Temperature Sensor ............................................ 21
Calibrating the Temperature Sensor ............................................ 21
Checking (Validating) the Instrument............................................ 22
Chapter - 6 Routine Operation ........................................................................ 26
6.1 Preparing the Instrument .............................................................. 26
Switching on the Instrument ........................................................ 26
Changing the Instrument Parameters ........................................... 27
6.2 Executing a Measurement ............................................................. 29
Determining the Blank ................................................................ 29
Resetting the Blank ..................................................................... 30
Supplying a Sample ................................................................... 30
Connecting a Temperature Sensor .............................................. 30
Starting the Measurement ........................................................... 31
Measurement ............................................................................ 31
After Measurement ..................................................................... 31
6.3 Measurement Results ................................................................... 32
Measurement Result Display ....................................................... 32
Printing the Displayed Measurement Result ................................... 32
Switching off the Instrument ........................................................ 32
Chapter - 7
Settings ....................................................................................... 33
7.1 System Settings ........................................................................... 33
Opening the Menu .................................................................... 33
Explanations .............................................................................. 34
Switching the Printer on and off ................................................... 35
Selecting the Language .............................................................. 36
Input of Date and Time .............................................................. 37
7.2 Instrument Settings ...................................................................... 38
Opening the Menu .................................................................... 38
Explanations .............................................................................. 39
Selecting the Scale ..................................................................... 40
Selecting the Weighted Sugar Sample .......................................... 40
Selecting the Polarimeter Tube .................................................... 42
Selecting the Temperature .......................................................... 43
2
7.3 Calibrating the Measurement Range............................................. 44
Opening the Menu .................................................................... 44
Canceling the Calibration ........................................................... 45
Resetting the Calibration ............................................................. 46
Performing the Calibration .......................................................... 46
Blank Measurement ................................................................... 46
Input of the Quartz Standard....................................................... 46
Input of the Quartz Temperature ................................................. 48
Measuring the Quartz Standard .................................................. 49
Accepting the Calibration ........................................................... 49
Rejecting the Calibration ............................................................ 50
7.4 Calibrating the Temperature Sensor .............................................. 51
Prerequisites .............................................................................. 51
Calling the Function ................................................................... 51
Measuring the Temperature ........................................................ 52
Input of the Thermometer Temperature ........................................ 53
7.5 Blank............................................................................................ 56
Calling the Blank Function .......................................................... 56
Resetting the Blank ..................................................................... 57
Measuring the Blank .................................................................. 57
Saving the New Blank................................................................. 58
Rejecting the New Blank ............................................................. 58
Chapter - 8 Maintenance, Care & Inspection ................................................... 59
8.1 Regular Maintenance and Care ..................................................... 59
Daily ......................................................................................... 59
Weekly ...................................................................................... 59
Yearly ....................................................................................... 59
When necessary ......................................................................... 59
8.2 Changing the Fuse ........................................................................ 60
8.3 Maintenance by Service................................................................ 60
Chapter - 9 Troubleshooting Table ................................................................. 61
Chapter - 10 Transport, Storage and Disposal .................................................. 62
Transport .................................................................................. 62
Storage ..................................................................................... 62
Disposal .................................................................................... 62
Chapter - 11 Appendix ...................................................................................... 63
3
11.1 Technical Specifications ............................................................... 63
11.2 Ordering Information .................................................................... 65
11.3 Consumables ................................................................................ 66
4
Chapter - 1 Introduction / 1.1 Application Examples
Chapter - 1 Introduction
The Polarimeter P3000 is very easy to operate and is designed for the fully automated
measurement of optical rotation.
The measurement sample is filled into the polarimeter tube, inserted into the sample
chamber and measured. The results are shown on the display and, if connected, printed
out on a printer.
The operator dialog is available in German and English.
1.1
Application Examples
Application areas for the polarimeter are, amongst
others:
• Pharmaceutical Industry
 Monitoring of chemical processes
 Purity control and determination of
concentrations
 Testing of substances listed in the German
Pharmacopoeia (DAB)
• Chemical Industry
 Purity control and determination of
concentrations
 Analysis of optically active components
(qualitative und quantitative)
 Determination of configuration changes
 Monitoring of chemical processes
• Food Industry
 Determination of concentrations
 Purity control
 Quality control
• Sugar and Sweetener-Industry
 Quality control of raw materials and end
products
 Determination of fructose and glucose
• Cosmetic Industry
• Beverage Industry / Breweries
5
Chapter - 1 Introduction / 1.2 Reading Information
1.2
Reading Information
Although instrument operation of the P3000 is easy, we recommend attentive reading of
this manual to ensure correct and optimal use of all functions.
Symbols
This manual uses the following symbols:
• Listings
 Instructions to do something
Text in italics refers to text in dialog windows.
 Reference to further information in this manual.
Finding Information
6
•
•
Look up in the table of contents.
Should a problem occur, refer to Chapter - 9
Troubleshooting Table
Chapter - 2 General Safety Instructions
Chapter - 2 General Safety Instructions
Hazards during normal specified operation:
Danger!
Electric shock by damaged instrument or power cable.
 Before switching on the instrument, make sure that
the instrument and the power cable are
undamaged.
 Only operate instruments that have been properly
installed and maintained.
Danger!
Electric shock by entering liquids.
 Before cleaning, switch off the instrument and
disconnect the power supply.
 Do not allow liquids to enter the instrument.
 Do not spray the instrument for cleaning.
 Make sure the instrument is completely dry before
connecting to the power supply.
Danger!
Electric shock
 Before changing the fuse, switch off the instrument
and disconnect the power cable.
Warning!
Damage to the retina by the LED light source.
 Do not look directly into the LED light source.
Careful!
Danger of pinching your hand.
 Close the sample chamber lid carefully.
7
Chapter - 2 General Safety Instructions
Careful!
Damage to electrical components by condensate
formation.
 Wait at least one hour after transporting the
instrument in a cooler environment (e.g. cold room
or outdoors) before connecting to the power
supply.
Careful!
Overheating damage.
 Do not place the instrument near a heat source
(e.g. heating).
 Do not expose to direct sunlight.
 Allow for unhindered air circulation.
Attention!
Aggressive chemicals may damage the instrument's
paint or the plastic housing!
 Clean the instrument with neutral cleaning agents
only.
 DO NOT use aggressive chemicals for cleaning,
such as strong or weak acids or bases, acetone,
formaldehyde, chlorinated hydrocarbons or
phenols.
 If the instrument comes into contact with aggressive
chemicals, clean immediately with a neutral
cleaning agent.
8
Chapter - 3 Instrument Description / 3.1 Scope of Delivery
Chapter - 3 Instrument Description
This chapter describes the following topics:
• Scope of delivery
• Optional accessories
• Instrument images
• Required reagents
• Screen description
• Measurement principle
3.1
Scope of Delivery
The following items are included in the delivery of the polarimeters P3000:
• Polarimeter P3000
• Power cable
• Touchscreen pen
• Polarimeter tubes:
1 x 100 mm glass tube with middle funnel
1 x 200 mm glass tube with middle funnel
•  User manual
3.2
Optional Accessories
•
•
3.3
Printer
various polarimeter tubes
Required Reagents
Calibration
Accuracy Check
Sample Dilution
For calibration of the instrument, quartz standards are
used.
 See Ordering Information.
For checking the accuracy of the instrument's
measurements you may use:
• Quartz standards
• Standard solutions with known concentrations
The solution used for sample dilution depends on the
sample material to be measured and should not itself
be optically active.
9
Chapter - 3 Instrument Description / 3.4 Instrument Images
3.4
Instrument Images
Instrument Front View of
P3000
Sample chamber port
Touchscreen user interface
Instrument Back View of
P3000
The P3000 provides the following connections:
Printer connection (for direct result output)
Power supply connection with power switch and
fuse
•
•
Power switch & fuse &
power supply connection
Polarimeter Tubes
10
Printer connection
(serial interface)
The polarimeter tube is available in two different
lengths:
• 100 mm
• 200 mm
• various other types optionally available
Chapter - 3 Instrument Description / 3.5 Screen Descriptions
3.5
Screen Descriptions
This chapter briefly describes the following screens of the polarimeter P3000:
Main Menu
In the main menu the measurements are started and
the results are displayed. The buttons lead to further
submenus.
Display window for results, unit, blank as well as
instrument and system settings.
Operation of buttons via touchscreen.
Explanation of Buttons
Opens the menus for :
•
System settings
•
Instrument settings
•
Calibration (measurement range,
temperature sensor)
•
Blank
Provides the following information:
•
Instrument and firmware settings
•
Date of last calibration
•
Version of user interface and data
base
11
Chapter - 3 Instrument Description / 3.5 Screen Descriptions
Prints the result shown on the display.
Starts the measurement.
Explanation of Display
Window
11.000 °Z Measurement result of the sample with
unit.
Blank
Blank used for calculation of the sample
result.
Connected printer is switched on.
The motor is switched on.
No display: motor is switched off. It
switches off automatically a few seconds
after the measurement.
The temperature sensor is connected to the
sample polarimeter tube and the
measured temperature during sample
measurement is 20.0 °C in this example.
 Explanations: see Chapter 7.4 Calibrating the
Temperature Sensor.
No temperature display: no temperature
sensor connected.
Size of the employed polarimeter tube.
Is selected in the instrument settings.
Selected wavelength (fixed setting).
12
Chapter - 3 Instrument Description / 3.6 Measurement Principle
3.6
Measurement Principle
The polarimeter measures the optical rotation of the orientation of plane-polarized light
caused by optically active substances. In principle, it consists of two polarization filters,
the polarizer and the analyzer, each of which allows only a certain orientation of planepolarized light to pass through.
The polarimeter measures the optical activity or the optical rotation of a chemical
substance.
The following equation defines the relation between the optical rotation, the specific
rotation and the sample concentration:
c = concentration [g/l]
α = measured optical rotation [°]
l = tube length [dm]
[α]T = specific rotation, dependent on temperature (°C) and wavelength
The optical rotation is, amongst others, dependent on the following parameters:
•
Type of sample
•
Concentration of the optically active components
•
Light wavelength
•
Temperature of sample
•
Tube length
13
Chapter - 4 Basic Operation / 4.1 Buttons
Chapter - 4 Basic Operation
The instrument is operated by a touchscreen display using four touchscreen buttons.
4.1
Buttons
Operation of the polarimeter is performed using these
four buttons:
Opens the submenus for :
System settings (printer, language, date/time)
Instrument settings (scale, polarimeter tube,
temperature)
• Calibration (measurement range, temperature
sensor)
• Blank
•
•
Returns to the main menu.
Selection of desired settings (system settings,
instrument settings, instrument calibration,
blank).
Opens the menu, e.g. for system settings.
14
Chapter - 4 Basic Operation / 4.1 Buttons
Opens the wiindow with the following information
about the P3000:
•
Instrument and firmware settings.
•
Date of last calibration.
•
Version of the user interface and data base.
Returns to the main menu.
Page up and page down in the
information pages.
Prints the result shown in the display.
Starts the measurement.
15
Chapter - 4 Basic Operation / 4.2 Defining the System Settings
4.2 Defining the System Settings
and
Switching between the parameters:
• Printer,
• Language,
• Day, Month, Year, Hour, Min, Sec.
Selection of the desired setting:
• on/off (printer)
• German/English (language)
• Date/time:
Counts up the respective selected
parameter (Day, Month, Year, Hour,
Min, Sec).
Accepts the set values and returns to the
main menu.
16
Chapter - 4 Basic Operation / 4.3 Defining the Instrument Settings
4.3
Defining the Instrument Settings
Selection of parameters (scale, polarimeter
tube, temperature).
Selection of parameter setting.
Accepts the set values and returns to the
main menu.
17
Chapter - 4 Basic Operation / 4.4 Performing the Calibrations
4.4 Performing the Calibrations
The functions for performing the calibrations of the measurement range and the
temperatue sensor are accessed using the button Calibration.
 Performing the calibrations see Chapter 7.4 Calibrating the Temperature Sensor.
Selection of calibration functions
(measurement range and temperature
sensor).
Returns to the settings menu.
Accepts the set values and returns to the
main menu.
18
Chapter - 5 Initial Startup / 5.1 Installation
Chapter - 5 Initial Startup
This chapter covers the following topics:
• Installation
• Preparing the instrument after installation
5.1
Installation
Choosing a Suitable
Location
 Place the instrument on a level and stable surface.
Please note!
The location should be free of vibrations and tremors.
Do not expose the instrument to direct sunlight.
Both can lead to faulty measurement results.
Connecting a Printer
 Connect the printer to the serial interface.
 Plug in the printer's power cable.
Connecting the Polarimeter
to the Power Supply
Permitted power supply: 100 -240 V, 50/60 Hz.
 Connect the polarimeter's power cable.
19
Chapter - 5 Initial Startup / 5.2 Preparing the Instrument after Installation
5.2
Preparing the Instrument after Installation
After installation the instrument is basically ready for operation.
Only if a temperature sensor is used, the sensor must first be calibrated before
performing the first measurement
A.Krüss recommends validation of the P3000 after transport and installation.
Warning!
Damage to the retina by the LED light source.
 Do not look directly into the LED light source in the
sample chamber.
Switching on the
Instrument
 Switch on the polarimeter (switch is located at the
back of the instrument).
Stabilizing the light source!
After switching on the instrument the light source must
reach thermal equilibrium, i.e. to achieve highest
precision, you should wait approx. 10 - 15 minutes
before starting the first measurement.
Switching on the Printer
If a printer is connected, it must be switched on in the
system settings as follows:
 Open the settings menu with
.
 Use the buttons  and  to select the system
settings and press
.
 Select the printer with the buttons  and .
 Switch the printer on with the button .
 Accept the settings with
20
.
Chapter - 5 Initial Startup / 5.2 Preparing the Instrument after Installation
Connecting the
Temperature Sensor
 If desired, connect the temperature sensor.
Calibrating the
Temperature Sensor
If a temperature sensor is connected, the sensor must
first be calibrated before operating the instrument.
The calibration consists of two temperature
measurements:
• Temperature measurement with the connected
temperature sensor
• Temperature measurement with a specified
thermometer.
The P3000 determines the temperature difference
between the sensor and the specified thermometer and
corrects the deviation of the sensor for all following
measurements.
 Performing the calibration: see Chapter 7.4 Calibrating the
Temperature Sensor.
21
Chapter - 5 Initial Startup / 5.2 Preparing the Instrument after Installation
Checking (Validating) the
Instrument
Although the P3000 is calibrated ex works, the
instrument's accuracy should always be checked after
transport.
The instrument check is carried out as follows:
• Set the instrument parameters
• Measure the blank
• Measure the quartz standard or a standard solution
of known concentration.
• If the results show a significant deviation from the
nominal values of the quartz standards or the test
standards, the measurement range must be recalibrated.
Setting the Instrument
Parameters
The instrument's default settings are as follows:
•
Scale = °
•
Polarimeter tube = 100.0 mm
•
Temperature °C
If desired, these settings can be changed as follows.
 Open the settings menu with
.
 Select the Instrument settings with the buttons 
and  and press
22
.
Chapter - 5 Initial Startup / 5.2 Preparing the Instrument after Installation
 Use the buttons  and  to select the desired
instrument settings (scale, polarimeter tube or
temperature).
 Set the desired parameter with the button  .
 Exit the selected instrument setting with the buttons
 and  and select the next instrument setting that
you want to change.
 Exit the menu Instrument Settings
with
Determining the Blank
.
Depending on whether the accuracy is checked using a
quartz standard or a standard solution, the blank
determination is performed differently as follows:
• When performing the accuracy measurement with a
quartz standard, the blank measurement is carried
out with the sample chamber empty.
• When performing the accuracy measurement with a
standard solution, the blank measurement is
carried out with an empty polarimeter tube (100
mm / 200 mm, selected in the instrument settings).
The determined blank is then subtracted from all
following measurement results.
 Open the system settings with
.
 Select Blank using the buttons  and .
23
Chapter - 5 Initial Startup / 5.2 Preparing the Instrument after Installation
 Leave the sample chamber empty or insert an
empty polarimeter tube into the sample chamber.
 Close the lid.
 Start the blank measurement with Start.
 To save the blank value press Save.
The blank is saved and the result is shown in the main
menu.
24
Chapter - 5 Initial Startup / 5.2 Preparing the Instrument after Installation
Measuring the Standard
 Insert the quartz standard into the sample
chamber.
or
 Fill the standard into the polarimeter tube.
 Close the sample chamber
 Start the measurement with
.
The measurement result is displayed.
If the result matches the value of the quartz standard or
the value of the standard solution, you may start with
routine operation.
If the results show larger deviations from the nominal
values, the measurement range should be recalibrated.
 See 7.3 Calibrating the Measurement Range.
25
Chapter - 6 Routine Operation / 6.1 Preparing the Instrument
Chapter - 6 Routine Operation
This chapter supplies information on the following topics:
•
•
•
•
•
6.1
What to do after switching on the instrument.
How to prepare measurements.
How to start the measurement.
What must be done after measuring.
How to print out the results.
Preparing the Instrument
Switching on the
Instrument
Warning!
Damage to the retina by the LED light source.
 Do not look directly into the LED light source in the
sample chamber.
 Switch on the polarimeter.
Stabilizing the light source!
After switching on the instrument the light source must
reach thermal equilibrium, i.e. to achieve highest
precision, you should wait approx. 10 - 15 minutes
before starting the first measurement.
 Switch on the printer, if desired.
The main menu opens. The last selected instrument
parameters are set.
26
Chapter - 6 Routine Operation / 6.1 Preparing the Instrument
Starting from the main menu, all necessary settings for
a measurement are defined.
Changing the Instrument
Parameters
Before starting a measurement, the following
instrument parameters can be changed:
•
Scale (unit)
•
Polarimeter tube (100 mm / 200 mm)
•
Temperature (°C / °F / off)
 For a description of the instrument parameters see
Chapter 7.2 Instrument Settings.
 Open the settings menu with
.
Select Instrument settings with the buttons  and .
Please note!
After changing the polarimeter tube size, a new blank
must be determined with the selected polarimeter tube.
 See Chapter 7.5 Blank.
27
Chapter - 6 Routine Operation / 6.1 Preparing the Instrument
 Open the menu with the instrument settings
with
.
 Select the desired instrument settings (scale,
polarimeter tube or temperature) using the buttons
 and .
 Set the desired parameter with .
 Exit the menu Instrument settings with
28
.
Chapter - 6 Routine Operation / 6.2 Executing a Measurement
6.2 Executing a Measurement
After changing the polarimeter tube, changing of the dilution medium or after opening
the polarimeter tube, a blank measurement of the polarimeter tube must be performed
first.
Determining the Blank
 Open the system setting with
.
 Select Blank with the buttons  and  .
 Insert the empty polarimeter tube into the sample
chamber.
 Close the lid of the sample chamber.
 Press Start.
 Accept the measured blank with Save.
The blank is saved and is subtracted from all following
measurement results.
 For a detailled description see Chapter 7.5 Blank.
29
Chapter - 6 Routine Operation / 6.2 Executing a Measurement
Resetting the Blank
If the saved blank should not be considered in the
following sample measurements, the blank must be
reset to zero.
 Open the system setting with
.
 Select Blank with the buttons  and  and press
.
 Press Reset.
 The blank is reset to zero.
Supplying a Sample
 Fill the sample into the polarimeter tube with which
the blank has been determined.
The polarimeter tube must be completely filled and
virtually free of air bubbles.
Please note!
Faulty measurement results are caused by air bubbles!
 A possibly existing air bubble must not interfere
with the tube's optical path.
Connecting a Temperature
Sensor
 If desired, connect the temperature sensor.
Careful!
Danger of pinching your hand.
 Close the sample chamber lid carefully.
 Close the lid of the sample chamber.
30
Chapter - 6 Routine Operation / 6.2 Executing a Measurement
Starting the Measurement
After inserting the polarimeter tube filled with the
sample, the measurement can be started.
Temperature control!
If there is a greater temperature difference between the
sample and the ambient temperature, you should wait
a while before starting the measurement. If the sample
has not reached ambient temperature, faulty results are
obtained.
 Press
Measurement
.
The measurement is started.
A single measurement is executed.
is
The motor is operating when the symbol
displayed.
If the motor was switched off, a startup phase is
required first.
 The measurement is then executed.
If a temperature sensor is connected, the actual
temperature is measured at the polarimeter tube and
displayed.
Symbol
**.* °C
20.0 °C
No display
After Measurement
Explanation
Temperature sensor is connected to
the polarimeter tube. No value
available yet.
Actual temperature at the polarimeter
tube.
If the temperature sensor is calibrated,
the corrected temperatue is displayed.
If the sensor is not calibrated, the
uncorrected temperature is shown.
No temperature sensor connected.
 Remove the polarimeter tube from the sample
chamber.
 Clean the polarimeter tube immediately after
measurement with dest. water or an appropriate
solvent.
31
Chapter - 6 Routine Operation / 6.3 Measurement Results
After cleaning, the sample tube is again available for
the next sample measurement.
The motor of the polarimeter is automatically switched
off after a few seconds.
6.3
Measurement Results
Measurement Result
Display
The measurement result is displayed together with the
unit.
Printing the Displayed
Measurement Result
 Press
.
The measurement result shown on the display is printed
out on the connected printer.
Switching off the
Instrument
 Switch off the printer.
 Switch off the polarimeter (switch located on the
back of the instrument).
32
Chapter - 7 Settings / 7.1 System Settings
Chapter - 7 Settings
This chapter covers the following topics:
• How to switch the printer on and off.
• How to select the language.
• How to set date, time and language.
• How to set the scale and the polarimeter tube.
• How to switch the temperature measurement on
and off.
• How to calibrate the measurement range.
• How to calibrate the temperature sensor.
• How to reset the measurement range (to ex works
setting)
• How to perform a blank measurement.
• How to reset a blank.
7.1
System Settings
All required system settings are defined in this menu:
• Printer
• Language
• Date and time
Opening the Menu
 Open the settings menu with
.
33
Chapter - 7 Settings / 7.1 System Settings
 Select System settings with  and  .
 Open the system settings menu with
.
Explanations
Settings/Buttons
Explanations
Printer
The connected printer can be switched on and off.
Language
The languages German and English are available.
Date/Time
Date and time are set.
and
Switching between the various parameters: Printer,
Language, Day, Month, Year, Hour, Min, Sec.
Selection of the desired setting:
on/off, German/English
Exception Date/Time: counts up the selected
respective parameter (Day, Month etc.).
Accepts the settings, closes the dialog window and
opens the main menu.
34
Chapter - 7 Settings / 7.1 System Settings
Switching the Printer on
and off
If a printer is connected, it can be switched on and off
with the P3000.
 Open the system settings with
.
 Select Printer using the buttons  and .
 Switch the printer on or off with the button  .
 Exit the menu System settings with
.
The selected parameter is accepted.
35
Chapter - 7 Settings / 7.1 System Settings
Selecting the Language
The languages German and English are available.
 Open the system settings with
.
 Select Language using the buttons  and .
 Select the language German or English with the
button  .
 Exit the menu System settings with
The selected parameters are accepted.
36
.
Chapter - 7 Settings / 7.1 System Settings
Input of Date and Time
 Open the system settings with
.
 Select Date/Time using the buttons  and .
 Further select Day , Month, Year and Hour, Min as
well as Sec with the buttons  and .
 Set the desired number with the button .
 Using the buttons  and  switch to the next
desired setting (day, month, year, etc.).
 Exit the date/time setting with the button .
 Exit the menu System settings with
.
The selected parameters are accepted.
37
Chapter - 7 Settings / 7.2 Instrument Settings
7.2
Instrument Settings
In the instrument settings the parameters for the measurement are set.
Parameters for the measurement are:
•
•
•
•
Scale (unit)
Length of the polarimeter tube [mm]
Wavelength [nm]
Currently only the wavelength 589 nm is available.
Actual temperature at the polarimeter tube [°C / °F
/ off]
Except for the wavelength, all required parameters for the measurement are selected in
the instrument settings.
Opening the Menu
 Open the settings menu with
.
 Select Instrument settings using the buttons
 and .
 Open the menu with
38
.
Chapter - 7 Settings / 7.2 Instrument Settings
Explanations
Settings/Buttons
Explanations
Scale
Selection of the unit:
°
Opt. Rotation.
Actual measured value.
°Z
International sugar scale.
For this unit the weighted sugar sample in
g/cm³ is selected:
• 6.5 g/cm³
• 13.0 g/cm³
• 26.0 g/cm³
Polarimeter tube
Selection of thr pre-defined polarimeter tube length:
• 100.0 mm
• 200.0 mm
Temperature
If a temperature sensor is connected, the
temperature at the polarimeter tube is measured.
The temperature unit is selectable.
°C / °F
Selection of the temperature unit for
temperature display. Selecting the unit
automatically activates temperature
display.
off
No temperature sensor connected or no
temperature display.
39
Chapter - 7 Settings / 7.2 Instrument Settings
Switching between the parameters: Scale,
Polarimeter tube, Temperature.
and
Selection of the desired parameter settings:
°/°Z, 100.0 mm/200.0 mm, °C/°F/off.
The settings are accepted, the dialog window is
closed and the main menu opens.
Selecting the Scale
Selection of the scale. There are two scales available:
Opt. Rotation (°)
International Sugar Scale (°Z).
If you choose the international sugar scale, you must
also select an additional weighted sugar sample.
•
•
 Open the instrument settings with
.
 Select Scale using the buttons  and .
 Select the desired scale (°/°Z) with the button .
Selecting the Weighted
Sugar Sample
 Select the scale °Z with the button .
After selecting the scale °Z the dialog window for
selection of the weighted sugar sample in g/cm³
opens:
40
Chapter - 7 Settings / 7.2 Instrument Settings
 Select the desired weighted sugar sample with the
buttons  and .
 Accept the selection with
.
The dialog window closes and the menu Instrument
settings opens again.
 Exit the menu Instrument settings with
.
The selected unit is accepted.
41
Chapter - 7 Settings / 7.2 Instrument Settings
Selecting the Polarimeter
Tube
There are two pre-defined polarimeter tube lengths
(100.0 mm und 200.0 mm) available.
 Open the instrument settings with
.
 Select Polarimeter tube using the buttons
 and .
 Select the desired polarimeter tube with the button
.
 Exit the menu Instrument settings with
The polarimeter tube selection is accepted.
42
.
Chapter - 7 Settings / 7.2 Instrument Settings
Selecting the Temperature
If a temperature sensor is connected, the temperature
at the polarimeter tube is measured. The temperature
unit is selectable. Unit selection automatically activates
the display, e.g.:
20.0 °C.
 Open the instrument settings with
.
 Select Temperature using the buttons  and .
 Select the desired temperature unit with the button
.
 Exit the menu Instrument settings with
.
The temperature settings are accepted.
43
Chapter - 7 Settings / 7.3 Calibrating the Measurement Range
7.3
Calibrating the Measurement Range
The calibration consists of two measurements:
•
•
Blank measurement of the empty sample chamber.
Quartz standard measurement.
For the quartz standard measurement the optical rotation of the quartz standard at 20
°C and the actual temperature of the quartz are entered. Using this data the polarimeter
automatically determines the nominal value for the optical rotation at the prevailing
actual temperature.
Calibration should be performed in regular intervals. Calibration should be performed
immediately, if result accuracy fails to lie in the desired range.
Opening the Menu
 Open the settings menu with
.
 Select Calibration with the buttons
 and .
 Open the dialog window Calibration
with
44
.
Chapter - 7 Settings / 7.3 Calibrating the Measurement Range
 Select Measurement range with the buttons  and
.
 Start the calibration with
.
The first calibration dialog window opens.
Canceling the Calibration
 Press Cancel.
The calibration is canceled and the previous calibration
is maintained.
45
Chapter - 7 Settings / 7.3 Calibrating the Measurement Range
Resetting the Calibration
 Press Reset.
The calibration is reset to the ex works setting.
Performing the Calibration
For calibration the polarimeter blank is measured with
the empty sample chamber first.
 Remove the polarimeter tube from the sample
chamber and close the lid.
 Start the blank measurement with Measure.
Blank Measurement
The polarimeter blank is determined.
Input of the Quartz
Standard
46
After the measurement the dialog window for input of
the quartz standard opens first:
Chapter - 7 Settings / 7.3 Calibrating the Measurement Range
Exits the calibration menu and returns to the
main menu. The previous calibration is
maintained.
Selection of the desired
• value, decimal point, sign
• functions:
CLR = Deletion of the complete value.
DEL = Deletion of the last input character.
Accepts the value selected with the button 
or executes the function (CLR / DEL).
Accepts the input value, closes the window
and opens the dialog window for input of the
quartz temperature.
 Input of the quartz standard's optical rotation.
 Accept the value with
.
The dialog window for input of the quartz temperature
opens.
47
Chapter - 7 Settings / 7.3 Calibrating the Measurement Range
Input of the Quartz
Temperature
 Enter the actual temperature of the quartz.
Input as described above.
 Finish the entry with
.
The input values (standard and temperature) including
the compensated quartz value are shown in the
following window.
Quartz Standard
48
Input value of the quartz-standard
(nominal temperature 20°C)
Chapter - 7 Settings / 7.3 Calibrating the Measurement Range
Quartz temp.comp. Opt. rotation of the quartz
standard at the actual
temperature.
Quartz Temperature Input actual temperature of the
quartz standard.
Measuring the Quartz
Standard
 Insert the quartz standard into the sample
chamber.
 Close the sample chamber.
 Press Measure.
The quartz standard is measured.
The measured result is calculated and displayed.
The calibration results are not directly connected with
the nominal values of the quartz standards.
Example:
Nominal Value of quartz standard = 29,600°, but
calibration point found = 22,457°
Accepting the Calibration
 Accept the calculated calibration values shown on
the display with
.
The old calibration value is overwritten with the new
value. The date of the calibration is saved in the
instrument.
49
Chapter - 7 Settings / 7.3 Calibrating the Measurement Range
Please note!
The date of the last calibration can be called up any
time with the button
Rejecting the Calibration
in the main menu.
 Press Reset to reject the calculated calibration
value shown on the display.
The old calibration remains valid. The new calibration
is discarded.
50
Chapter - 7 Settings / 7.4 Calibrating the Temperature Sensor
7.4
Calibrating the Temperature Sensor
The calibration consists of two temperature measurements:
•
•
Temperature measurement with the temperature
sensor
Temperature measurement with a specified
thermometer.
The temperatue difference between the sensor and the specified thermometer is
determined and compensated.
The temperature sensor must be calibrated:
•
•
•
Prerequisites
before the first use of the sensor
once a year
if necessary, i.e. when result accuracy lies outside of
the expected range.
For calibration you need:
• a temperature sensor
• a certified thermometer
• a vessel with water
Calling the Function
 Open the settings menu with
.
 Select Calibration with the buttons  and  and
press
.
The dialog window Calibration opens.
51
Chapter - 7 Settings / 7.4 Calibrating the Temperature Sensor
 Select Temperature sensor with the buttons  and
 and press
.
The dialog window Calibration temperature sensor
opens.
Display Explanation
Explanation of the Buttons
Nominal
value:
Measured
value:
Input field for the temperature measured
with the certified thermometer.
Display of the temperature measured by
the temperature sensor.
This value cannot be changed.
Opens the dialog window for input of
the nominal value (temperature
measured with the certified
thermometer).
Calibration of the temperature sensor is
reset.
Measuring the
Temperature
 Connect the temperature sensor.
 Position the temperature sensor and the certified
thermometer in a beaker with water.
 Wait until the display Measured value is stable
over a longer period of time.
52
Chapter - 7 Settings / 7.4 Calibrating the Temperature Sensor
Wait for thermal equilibrium!
If thermal equilibrium has not been reached when
entering the nominal value, the calibration may lead to
faulty results.
Sensor, thermometer, water and room temperature
must be the same. If the temperature display is stable
over a longer period of time, thermal equilibrium is
given.
Input of the Thermometer
Temperature
 Make a note of the temperature measured with the
specified thermometer.
 Press the button Nominal.
 Enter the temperature measured with the specified
thermometer as follows:
 Select the number with the button  and accept
the selected number with #.
 Repeat this procedure until the value has been
completely entered.
 Save the nominal temperature with
.
53
Chapter - 7 Settings / 7.4 Calibrating the Temperature Sensor
 Accept the nominal temperature with
Nominal
temperature
Actual
temperature
.
Input of the value measured with the
specified thermomter.
Value measured with the temperature
sensor.
 Press Ok.
The temperature difference is calculated and all
following temperatures measured with the sensor are
corrected with this value. The program returns to the
main menu.
54
Chapter - 7 Settings / 7.4 Calibrating the Temperature Sensor
The currently measured corrected temperature is
displayed.
55
Chapter - 7 Settings / 7.5 Blank
7.5
Blank
The function Blank allows you to perform a new blank measurement and to reset a
saved blank value to zero.
A blank measurement must be performed:
•
•
•
after each change of the polarimeter tube
after changing the dilution medium
after opening the polarimeter tube
Calling the Blank Function
 Open the setting menu with
.
Select Blank using the buttons  and .
 Start the function Blank with
The first dialog window opens:
56
.
Chapter - 7 Settings / 7.5 Blank
Resetting the Blank
If the saved blank should not be considered in the
following sample measurements, the blank must be
reset to zero.
 Press Reset.
Measuring the Blank
 Insert the empty / with dilution medium filled
polarimeter tube which will be used for the
following measurements into the sample chamber.
 Close the lid.
 Start the blank measurement with Start.
57
Chapter - 7 Settings / 7.5 Blank
Saving the New Blank
If you would like to accept the new blank shown on the
display:
 Press Save.
The new blank is saved and the main menu showing
the new blank value opens.
Rejecting the New Blank
If you do not want to accept the blank value shown on
the display:
 Press Cancel.
The new blank is discarded and the old blank is
maintained.
58
Chapter - 8 Maintenance, Care & Inspection / 8.1 Regular Maintenance and Care
Chapter - 8 Maintenance, Care & Inspection
This chapter covers the following topics:
•
•
•
8.1
What to do daily / weekly / yearly / when necessary
Changing the fuse
Maintenance by service
Regular Maintenance and Care
The instrument is virtually maintenance-free.
Daily
 After completing the measurements clean the
polarimeter tube.
Weekly
 Clean the display and housing with a commercial
cleaning or disinfecting agent.
Yearly
 Calibrate the temperatue sensor.
 To perform see Chapter 7.4 Calibrating the Temperature
Sensor.
When necessary
 Check the accoracy of the polarimeter.
 To perform see Chapter 6.1 Preparing the Instrument.
 Calibrate the polarimeter.
 To perform see Chapter 7.3 Calibrating the Measurement
Range.
59
Chapter - 8 Maintenance, Care & Inspection / 8.2 Changing the Fuse
8.2 Changing the Fuse
Please note!
Disconnect the power plug before changing the fuse.
 Disconnect the power plug.
 Change the fuse above the power switch.
 Re-connect the power plug.
8.3 Maintenance by Service
The Polarimeter P8000 requires no regular maintenance by service.
If the instrument is malfunctioning, please contact:
A.Krüss Optronic GmbH
Alsterdorfer Straße 276-278
22297 Hamburg GERMANY
Tel. +49 (0)40 - 514317-0 Fax: +49 (0)40 - 514317-60
Internet: www.kruess.com Email: [email protected]
60
Chapter - 9 Troubleshooting Table
Chapter - 9 Troubleshooting Table
This chapter offers help to eliminate possibly occuring errors quickly.
Error
Possible causes
Remedy
Faulty
measurement
results
•
Glass plates at the ends of the •
polarimeter tube are dirty or
stained.
Clean the glass plates.
•
Polarimeter tube dirty.
Clean the polarimeter tube.
•
Air bubbles in the optical path •
of the polarimeter tube.
Fill in additional sample into
the polarimeter tube.
•
Sample liquid extremely dark.
Dilute the sample.
•
Saved blank was not measured •
with the current polarimeter
tube.
•
•
Measure the blank with the
currently used polarimeter
tube.
 See Chapter 7.5 Blank.
•
Calibration data is obsolete.
•
Re-calibrate the measurement
range.
 See Chapter 7.3 Calibrating the .
•
Calibration data of the
temperature sensor faulty
•
Re-calibrate the temperature
sensor.
 See Chapter 7.4 Calibrating the
Temperature Sensor.
Inferior precision •
Polarimeter was not cleaned
thoroughly between sample
measurements.
•
Clean the polarimeter tube
thoroughly after each
measurement with dest. water
or an appropriate solvent.
•
Sample has not yet reached
room temperature.
•
Wait until the sample has
reached room temperature.
•
Fuse blown
•
Change the fuse.
Instrument
cannot be
switched on
 See Chapter 8.2 Changing the
Fuse.
61
Chapter - 10 Transport, Storage and Disposal
Chapter - 10
Transport
Transport, Storage and Disposal
 For transport purposes use the original packaging
only.
 Transport must be carried out at ambient
temperatures between -20°C and +60°C and at
max. 95% relative humidity.
Storage
Disposal
62
Storage for longer periods of time must be carried out
at ambient temperatures between +5°C and +45°C
and at max. 95% relative humidity.
For disposal of the product please observe the
corresponding legal regulations.
Information for disposal of electrical and electronic
equipment in the European Community.
Electrical devices are disposed of according to national
regulations on the basis of the EC Directive
2002/96/EC on waste electrical and electronic
equipment (WEEE).
Accordingly, all equipment delivered in the business-tobusiness sector after August 13, 2005, which applies to
this product, cannot be disposed of as municipal or
domestic waste.
These devices are marked with the adjoining symbol.
Please observe the corresponding legal regulations for
disposal.
Because regulations differ from country to country, we
recommend contacting your supplier, if necessary.
In Germany, compulsary labeling is in effect since
March 23, 2006. For all equipment delivered from
August 13, 2005 onwards, the manufacturer must offer
an appropriate option for returns.
For all equipment delivered before August 13, 2005
the last user is responsible for proper disposal.
Chapter - 11 Appendix / 11.1 Technical Specifications
Chapter - 11 Appendix
11.1 Technical Specifications
P3000
Measurement range
Measurement unit
Resolution
Accuracy
± 90°
± 259°Z
Opt. rotation [°]
Int. sugar scale [°Z]
0.01°
0.01 °Z
± 0.01°
± 0.01 °Z
Reproducibility
0.01°
Measurement time
approx. 1 bis 3 sec.
Light source
1 LED with filter 589 nm
Wavelength
589 nm
others optional
Wavelength selection
one fixed wavelength
Connection for
temperature sensor
Special polarimeter tube with temperature sensor PT100 required
Temperature
measurement
0..99.9 °C
Temperature resolution 0.1 °C
Temperature accuracy ± 0.2 °C
Temperature
measurement point
Sample in tube
Max. tube length
220 mm
63
Chapter - 11 Appendix / 11.1 Technical Specifications
Sample transmissibility min 0.1% (OD3)
Calibration
automatically (menu guided)
Display
LCD TFT 5.7“ 320x240 Pixel
User interface
Touchscreen
Interfaces
RS232 (Printer)
Power supply
100 - 240 V −, 50/60 Hz
Printer connection
RS-232 serial interface
Dimensions
65 x 20 x 36 cm
64
Chapter - 11 Appendix / 11.2 Ordering Information
11.2 Ordering Information
Name of Item
Description
Order no.
Polarimeter P3000
P3000
Glass tube (length 100mm)
P1000-100
Glass tube (length 200mm)
P1000-200
Glass tube (length 100mm with
funnel)
P1000-110
Glass tube (length 200mm with
funnel)
P1000-210
Stainless steel tube with filler tube,
temperature-controllable (length
100mm)
P8-100ET
Stainless steel tube with filler tube,
temperature-controllable (length
200mm)
P8-200ET
Flow-thru tube, temperaturecontrollable, with temperature sensor
PT-100 (length 100mm)
P8-100ETT
Flow-thru tube, temperaturecontrollable, with temperature sensor
PT-100 (length 200mm)
P8-200ETT
Stainless steel flow-thru tube with
funnel and overflow pipe (length
100mm)
P1000-D100
Stainless steel flow-thru tube with
funnel and overflow pipe (length
200mm)
P1000-D200
Premium quartz control plate for
polarimeter calibration
certifiable
Premium quartz control plate for
polarimeter calibration
certifiable
Premium quartz control plate for
polarimeter calibration
certifiable
Premium quartz control plate for
polarimeter calibration
certifiable
Premium quartz control plate for
polarimeter calibration
certifiable
Premium quartz control plate for
polarimeter calibration
certifiable
Economy quartz control plate for
polarimeter calibration
+34° (+/- 1°) . +99 °Z (+/- 1 °Z)
P8000-410
In °: -10° (+/- 1°). In °Z: -30° (+/- 1 °Z)
P8000+408
In °: +8° (+/- 1°). In °Z: +25° (+/- 1 °Z)
P8000+417
In °: +17° (+/- 1°). In °Z: +50° (+/- 1 °Z)
P8000+426
In °: +26° (+/- 1°). In °Z: +75° (+/-1 °Z)
P8000+434
In °: +34° (+/- 1°). In °Z: +99° (+/- °Z)
P8000+433
In °: +33° (+/- 1°). In °Z: +95° (+/- °Z)
P1000+534
not certifiable
65
Chapter - 11 Appendix / 11.3 Consumables
Economy quartz control plate for
polarimeter calibration in °:
+17° in °Z: +49 °Z not certifiable
P1000+517
Economy quartz control plate for
polarimeter calibration in °:
-17° in °Z: -49 °Z not certifiable
P1000-517
Economy quartz control plate for
polarimeter calibration in °:
-34° in °Z: -99 °Z not certifiable
P1000-534
Please contact us if you require a different type of polarimeter tube.
Glass coverplate for polarimeter tube
Pack with 2 pcs.
Spare sodium lamp
P1000-150
P1000-300
11.3 Consumables
Name of Item
Description
Printer for normal paper
24-characters
CBM 910
Paper roll
for printer CBM910
CBM910P
Ink ribbon
for printer CBM910
CBM910F
A.Krüss Optronic GmbH
Alsterdorfer Straße 276-278
22297 Hamburg GERMANY
Tel. +49 (0)40 - 514317-0 Fax: +49 (0)40 - 514317-60
Internet: www.kruess.com Email: [email protected]
66
Order no.
Warranty Conditions
A. KRUESS Optronic warrants that the instruments of the digital polarimeter family will
be free from defects in materials and workmanship after the date of delivery for a time
period of 24 months. During this warranty period A. KRUESS Optronic will repair or
replace products which fall under the warranty conditions. For warranty repairs or
service the instrument must be returned to A. KRUESS Optronic. In the case of warranty
repairs A. KRUESS Optronic will bear the delivery costs. For all other deliveries the
customer will bear the delivery costs.
A. KRUESS Optronic warrants that the hardware which A. KRUESS Optronic has
determined for use with this instrument will operate free from defects when applied
according our manufacturer's instructions.
A. KRUESS Optronic does not warrant error-free and uninterrupted operation of the
instrument or that this user manual is free of error. A. KRUESS Optronic will not be
liable for consequential damages.
Limited Warranty:
The preceding warranty does not apply to errors and defects caused by improper
handling, software not supplied by A. KRUESS Optronic, modification, abuse or by
operation outside of the stated environment or by unauthorized maintenance.
Further claims are excluded and will not be accepted. A. KRUESS Optronic explicitly
does not warrant the applicability or the economic use for certain applications.
A. KRUESS Optronic reserves the right to change this user manual and the technical
specifications of the described instrument at any time.
This digital polarimeter is only then ready for shipment when it is appropriately packed
in the complete original packaging including the plastic moldings. If necessary, please
order a replacement packaging from your supplier.
A.Krüss Optronic GmbH
Alsterdorfer Straße 276-278
22297 Hamburg GERMANY
Tel. +49 (0)40 - 514317-0 Fax: +49 (0)40 - 514317-60
Internet: www.kruess.com Email: [email protected]
67
Version 0.1 | Updated: December 2011 | Subject to changes and may contain errors.
A.KRÜSS Optronic GmbH
Alsterdorfer Straße 276-278
22297 Hamburg | Germany
Tel+49-(0)40-51 43 1 7- 0
Fax+49-(0)40-51 43 1 7- 60
[email protected]
Webwww.kruess.com
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