pco.flim pco.
user manual
pco.flim
pco.
PCO asks you to carefully read this manual before using the
pco.flim camera system and follow the instructions.
In case of any questions or comments, please contact us at PCO.
telephone
+49 (0) 9441 2005 50
fax
+49 (0) 9441 2005 20
email
[email protected]
postal address
PCO AG
Donaupark 11
93309 Kelheim, Germany
The cover photo shows an exemplary PCO camera system.
The lens is sold separately.
Copyright © 2016 PCO AG (called PCO in the following text), Kelheim,
Germany. All rights reserved. PCO assumes no responsibility for errors or
omissions in these materials. These materials are provided as is without
warranty of any kind, either expressed or implied, including but not limited to,
the implied warranties of merchantability, fitness for a particular purpose, or
non-infringement. PCO further does not warrant the accuracy or
completeness of the information, text, graphics, links or other items
contained within these materials. PCO shall not be liable for any special,
indirect, incidental, or consequential damages, including without limitation,
lost revenues or lost profits, which may result from the use of these materials.
The information is subject to change without notice and does not represent a
commitment on the part of PCO in the future. PCO hereby authorizes you to
copy documents for non – commercial use within your organization only. In
consideration of this authorization, you agree that any copy of these
documents, which you make, shall retain all copyright and other proprietary
notices contained herein. Each individual document published by PCO may
contain other proprietary notices and copyright information relating to that
individual document. Nothing contained herein shall be construed as
conferring by implication or otherwise any license or right under any patent or
trademark of PCO or any third party. Except as expressly provided, above
nothing contained herein shall be construed as conferring any license or right
under any PCO copyright. Note that any product, process, or technology in
this document may be the subject of other intellectual property rights
reserved by PCO, and may not be licensed hereunder.
Released May 2016 © PCO AG
pco.flim User Manual V1.10 © PCO AG, Germany
TABLE OF CONTENTS
TABLE OF CONTENTS
1. INTRODUCTION
1.1 INTENDED USE
5
5
2. SAFETY INSTRUCTIONS
6
3. SYSTEM COMPONENTS
7
4. INSTALLATION
8
4.1 CAMERA DRIVER
8
4.2 CAMWARE
9
5. QUICK START
10
5.1 PREPARATION
10
5.2 START
10
5.3 YOUR FIRST IMAGE
11
6. CAMWARE 3 SOFTWARE
12
6.1 INTRODUCTION / CONTENT
12
6.2 OVERVIEW OF ALL BUTTONS
13
6.3 CAMERA CONTROL WINDOW
14
6.3.1 TIMING TAB
15
6.3.2 SENSOR (SIZE) TAB
17
6.3.3 SENSOR (MISC.) TAB
18
6.3.4 RECORDING TAB
19
6.3.5 I/O SIGNALS TAB
20
6.4 CAMWARE FEATURES
21
6.4.1 DEMO MODE
21
6.4.2 FILE MENU
22
6.4.3 CAMERA MENU
26
6.4.4 ACQUISITION MENU
27
6.4.5 VIEW MENU
28
6.4.6 WINDOW MENU
30
6.4.7 HELP MENU
30
6.4.8 LOCAL MENU
31
6.4.9 ADDITIONAL FEATURES
32
3
7. [email protected] SOFTWARE
33
7.1 START
33
7.2 DATA FILES TAB
34
7.2.1 LOAD REFERENCE
34
7.2.2 LOAD DATA
37
7.2.3 EXPORT RESULTS
39
7.3 CAMERA MENU
40
7.3.1 MENU BAR
40
7.3.2 FIND CAMERA
40
7.3.3 SEQUENCE CONFIGURATION TAB
41
7.3.4 EXPOSURE TIME / NUMBER OF IMAGES
42
7.3.5 FUNCTION BUTTONS
42
7.4 VIEW MENU
45
7.5 LICENSING MENU
50
APPENDIX
51
A1 TECHNICAL DATA
52
A1.1 MECHANICAL DIMENSIONS
52
A1.2 DATA SHEET
53
A1.3 REVERSE OF THE PCO.FLIM
54
A2 USB 3.0
56
A2.1 HARDWARE RECOMMENDATIONS
56
A2.2 INSTALLATION OF THE USB 3.0 CARD
58
A3 WATER COOLING PCO.AQUAMATIC II
59
A3.1 SYSTEM COMPONENTS
59
A3.2 FIRST TIME INSTALLATION
60
A3.3 OPERATION
61
A3.4 DIMENSIONS
62
A4 IMAGE FILE FORMATS
63
A5 CUSTOMER SERVICE
65
A5.1 SERVICE
65
A5.2 MAINTENANCE
65
A5.3 RECYCLING
65
A5.4 TROUBLE SHOOTING
66
A6 INDEX
67
ABOUT PCO
68
4
1. INTRODUCTION
1. INTRODUCTION
Advantages of the pco.flim
The pco.flim camera system is the first luminescence
lifetime imaging camera using a special 2 tap CMOS image
sensor. It has all the required generation of frequency domain
signals included (5 kHz – 40 MHz) and also allows in a limited
frequency range (500 kHz – 40 MHz) the use of external
modulation signals. It has an USB 3.0 interface for image data
transfer and control of all camera operation modes. Further it
has a variety of trigger input / output signals for integration of
the camera into any application framework.
The camera’s main features are:
•
•
•
•
•
•
•
•
•
•
•
high resolution (1008 x 1008 pixel)
lifetimes from 100 ps to 100 ns
quantum efficiency up to 39%
>1000:1 (60dB) dynamic range
low noise of 45 e- rms
high frame-rate 90 fps (2-tap readout)
exposure times from 10 ns to 10 s
vibration-free water cooling
modulation frequency range 5 kHz to 40 MHz
USB3.0 interface
pixel size 5.6 µm x 5.6 µm
1.1 INTENDED USE
This camera system is designed for use by technicians, engineers
and scientists. It is a scientific measuring instrument, which provides
images. The camera may only be used according to the instructions
of this manual. The disclosures and operating conditions in these
operating
instructions
must be
respected.
Unauthorized
modifications and changes of the device are forbidden for safety
reasons.
Areas of Application
•
•
•
•
•
•
•
fluorescence lifetime imaging
life science
scientific imaging
pressure sensitive paint (PSP)
FRET
bioluminescence / chemoluminescence
imaging of bio markers (e.g. green fluorescent protein, GFP)
5
2. SAFETY INSTRUCTIONS
Please read the safety instructions completely and follow them
always.
DANGER
DAMAGED POWER CABLE OR POWER PLUG
Danger to life due to electric shock.
 Each time the camera is used, check the power cable for
damage.
WARNING
ELECTRIC SHOCK WARNING DUE TO VOLTAGE PARTS INSIDE
Risk of injury due to electric shock.
 Never slide any items through slits or holes into the camera.
CAUTION
CONDENSATION
Risk of injury due to electric shock if condensation enters the
camera.
 To avoid the risk of water condensation, protect the camera
against extreme changes of ambient temperature.
CAUTION
TRIPPING HAZARD
Risk of injury from tripping over loose cables.
 Never position the cable in a way that it could become a
tripping hazard.
NOTICE
HUMID OR DUSTY ENVIRONMENTS
Humidity, dust or X-rays could damage the camera.
 Never operate the camera in humid or dusty environments
or in places with high amounts of X-ray radiation.
NOTICE
JOLT & VIBRATION
To prevent damage to the camera, the system must be kept stable
and protected against strong jolts or vibrations.
 Use the mounting threads of the camera to mount the
camera stable.
NOTICE
LENS MOUNTING
Do not force the lens onto the camera.
 To protect the lens connector thread from damage, use
minimal force when attaching a lens to the camera.
NOTICE
LIQUIDS DAMAGE CAMERA
If liquids have penetrated the device.
 Immediately switch off the camera, separate it from power
line and contact our customer support.
NOTICE
DAMAGED CAMERA HOUSING
If the camera has been dropped or the casing is damaged.
 Immediately switch off the camera, separate it from power
line and contact our customer support.
NOTICE
IF CAMERA IS NOT WORKING PROPERLY
If, after thoroughly reviewing the instruction manual, the device is still
not operating properly.
 Immediately switch off the camera, separate it from power
line and contact our customer support.
6
3. SYSTEM COMPONENTS
3. SYSTEM COMPONENTS
The camera system includes the following parts.
Camera Head
C-mount optical connection:
The distance between the front edge of the c-mount and the sensor is
17.52 mm. For standard c-mount lenses and adapters.
DC Power Jack (connect to power supply)
USB 3.0 connector
Trigger Connector (see A1.3)
LED indicates camera status (see A1.3)
LED Green continuous=camera is ready
LED Yellow =boot loader
LED Orange = camera is armed/blinking while recording
LED Red continuous=error
Serial Number Tag
Mounting Thread
3/8''-16 UNC
1/4"- 20 UNC
M6
Power Supply
AC to 12V/DC power supply
USB 3.0 cable
USB-A/USB-B cable (5 m)
Digital Camera Tools (USB storage device content)
•
•
•
Camware: software for camera control & image acquisition
Camera driver & tools
Software development kit (SDK) & demo programs in C and C++
7
4. INSTALLATION
You will find all necessary files on the accompanying USB storage
device. You may also download the newest versions of our software,
camera driver and third party software drivers from our Website
(www.pco.de).
Minimum system requirements:
•
•
•
•
•
Clock speed > 2 GHz (Intel i5 Haswell or better)
RAM > 4 GB
Windows 7 or higher
1280 x 1024 pixel resolution display
USB 3.0
4.1 CAMERA DRIVER
Please start the USB 3.0 driver installation from your pco.usb stick.
Don’t connect the camera to your computer before driver is
completely installed.
Run the provided installation file and follow the instructions of the
installation wizard. 1
After finishing 2 the driver installation wizard please connect your
pco.flim camera to its power supply and via enclosed USB 3.0 cable
to your computer.
The first time the camera is connected via USB to your computer,
Windows will notify the user that a new device has been detected.
1
2
8
4. INSTALLATION
4.2 CAMWARE
The Camware Windows application software enables you to control
every camera parameter or setting. Images can be displayed on a
monitor and may be downloaded and stored. The USB storage
device contains the installation files for the software for latest
Windows operating systems in 32 & 64 bit.
After a successful installation, you will find the program file Digital
Camera Toolbox in your program directory and a Camware32/64
button on your desktop. Other helpful tools are also installed in the
same directory.
To uninstall the Camware program, please use the Software feature
under Windows’ System Control.
Please follow the installation wizard
-1
Install Camware as Admin to install to program folder, instead it
will be installed only to user folder.
-2 Then choose install directory.
-3 Choose Components: Select additional drivers for camera Link
Interface (not available for pco.flim).
-4
1
3
After the next two screens installation is complete.
2
-
4
9
5. QUICK START
In order to get familiar with your new camera and software it might be
helpful, if you first aim at an object that is easy to focus and that can
be seen at standard light conditions.
5.1 PREPARATION
• Computer is turned on
• Installation is finished (see chapter 4)
• An appropriate lens is attached (remove cap) or the camera is
attached properly to the microscope, spectrograph or other
scientific device
• Camera is connected to your computer
• Camera is connected to the power supply and ready (LED green)
5.2 START
Start Camware and the graphical user interface will start up:
NOTE
Always install latest Camware version to be able to use full
function of your pco camera.
10
5. QUICK START
5.3 YOUR FIRST IMAGE
Please follow the instructions:
1• Camware must be started.
2• Open view window (b/w ).
3• Start live preview.
4• Apply auto range peak.
5• You may adjust exposure time, aperture and focus.
6• Now you should clearly see the object in the window.
If you need to change exposure time
(e.g. the image is still either too dark or
too bright), please go to chapter 6.3.
If you want to record and save images,
please see chapter 6.2 and chapter 6.4
for detailed information.
NOTE
Live preview: Useful for fast and easy camera adjustment and
focusing. This command disables the Camera Control window if it
is opened (except exposure time setting).
11
6. CAMWARE 3 SOFTWARE
6.1 INTRODUCTION / CONTENT
PCO’s Camware is excellent software for camera control,
image acquisition and archiving of images in various file
formats.
Camware works with any kind of PCO camera. Please see
our Website for the latest version of this software.
Chapter 6.2. shows an overview of all software buttons and menus
of Camware.
Chapter 6.3 the Camera Control window. Camera Control is the
main interface for all camera settings:
6.3.1 Timing Tab
6.3.2 Sensor Size Tab
6.3.3 Sensor (Misc) Tab
6.3.4 Recording Tab
6.3.5 I/O Signals Tab
6.3.6 FLIM Tab
Timing /trigger modes/
synchronization
ROI / Sensor format / binning
CDI / Double Shutter / temperature
Recorder Aquire Mode / timestamp /
sequence trigger
Input and Output signal possibilities
External Tool
Chapter 6.4 introduces to further Camware features:
6.4.1 Demo Mode
6.4.2 File Menu
6.4.3 Camera Menu
6.4.4 Acquisation Menu
6.4.5 View Menu
6.4.6 Window Menu
6.4.7 Help Menu
6.4.8 Local (right-click)
6.4.9 Additional Features
12
No camera connected
Open, save and print files, Options…
Camera Control: Settings; Rescan…
Live preview; Acquire secquence;
Rec. memory settings; Recorder
settings
B/W or Color window; Convert
Control, Image Browser
Cascade; Tile; Arrange; Close all
Help file, About Camware
Zoom; Flip; Mirror; Rotate…
White Balance, Contrast, ROI by
Mouse
6. CAMWARE 3 SOFTWARE
6.2 OVERVIEW OF ALL BUTTONS
Menu Bar
See chapter 6.4.2- 6.4.7
Local Menu

Mouse right-click is explained in chapter 6.4.8
Toolbar
Prints the currently displayed image or comment window
Opens a b/w | color display window
Opens the camera control window (see chapter 6.3)
Opens the convert control (see chapter 6.4.5)
Auto range peak/crop (see chapter 6.4.5 Convert Control)
Live preview (see chapter 6.4.4)
Soft trigger captures a single image (see 6.3.1)
Cursor display
Cursor: pixel position and intensity value
Continuous auto range crop scaling
Recorder
Record START / STOP / PLAY
Opens the recorder settings window (see 6.4.4)
Replay Navigation
Image Browser: overview of the recorded images (see 6.4.5)
Multi Frame Window (see chapter 6.4.5 )
13
6.3 CAMERA CONTROL WINDOW
The camera control window can be opened by selecting the
proper command in the Camera-Menu or by the
corresponding button in the toolbar.
1
The camera control dialog always adapts
to the camera type connected. For the
pco.flim the camera control settings are
spread over four property sheets 1 ,
which will be explained below.
Changes to the camera control sheets
must be completed by pressing the
Accept button 2 .
3
In the information field 3 you can see
some information about the camera. Click
into the info field to change the displayed
views.
2
There are three avaiable views of the information field 3
Camera type
Actual temperature
Image size and frame-rate
NOTE
If the Accept button is not pressed, these changes will be ignored
and lost. If the changes are within the allowed condition limits of
the camera, a message is sent to the computer, the button
disappears and the green lamp will be highlighted. If an
impossible change was attempted, the camera will not accept the
change and the red lamp will be highlighted.
14
6. CAMWARE 3 SOFTWARE
6.3.1 TIMING TAB
Trigger Mode
Auto Sequence The camera will optimize
the image recording to achieve the best
possible frame-rate.
In the Auto Sequence exposure control
mode, the camera determines the fastest
possible frame-rate depending on the
adjusted exposure time and the required
readout time.
After a start command is given, the
sequential recording is started until a stop
command is given.
2
1
1
The following timing diagram shows the exposure sequencing of two
exposures times.
busy
exposure
tdel
tread,0
texp,1
toh
tdel
tread,1
texp,2
toh tdel
tread,2
toh
light enable
(gate)
tread,0 :
tread,2 :
first frame is emitted (not output, only for resetting rows)
readout of last exposure
ttd: trigger delay (incl. jitter)
tread: readout time
tdel: delay time
toh: overhead time
texp: exposure time
Maximum fps (Auto Sequence mode, i.e. ttd = 0):
1/fpsonetap = tdel + tread + texp +toh (for one tap)
Nrows: number of rows ≙ vertical ROI; FPSbothtaps = 2 * FPSonetap
Soft Trigger Single images can be recorded with this Camware
command. The single image can be acquired by pressing the Single
Trigger button. This button appears after pressing the Start Record
button (see 6.2). Other signals cannot influence this operating mode.
Timing parameter
Pixel clock 12.5 MHz
Pixelclock 25 MHz
Adjustable 0…1s
tdel
tread
texp
toh
ttd
31.2 µs * Nrows
20.96 µs * Nrows
10 ns…10 s
4.8 µs
≤ tdel + tread + texp + toh
15
External Exp. Start The image acquisition is triggered by an external
signal. It is also possible to force a software trigger for a test image
with the Single Trigger button.
In the External Exp. Start exposure control mode, single image
recording is started by the rising edge of the voltage signal at the
Trigger input (see appendix A1.3).
trigger
busy
exposure
ttd tdel
tread,
texp,
toh tdel
tread,
toh
light
enable
(gate)
texp,1 : number of exposures and readouts depends on sequence length
tread,0 : first frame is emitted (not output, only for resetting rows)
External Exp. Ctrl
Not available for pco.flim.
Timing 2
The exposure time can be adjusted in steps of 10 ns.
16
ttd
6. CAMWARE 3 SOFTWARE
6.3.2 SENSOR (SIZE) TAB
1
2
3
Binning 1
Not available for pco.flim.
Region of Interest 2
The ROI (Region of Interest) selects only a part of the sensor to be
read out, in order to speed up the frame-rate and to reduce the
amount of image data. The decreased image size you see within
Camware is a combination of reduced sensor resolution and software
downsizing (Soft-ROI). ROI by mouse see 6.4.9). The frame-rate only
increases, if the vertical resolution is decreased.
ROI Step sizes
increments horizontal:
increments vertical:
minimum ROI:
Sensor Format
16 pixel steps
1 pixel steps
16 x 4 pixels
3
This sensor implementation provides the option to either read out the
standard size of 1008 x 1008 pixels (effective pixels recommended by
the sensor manufacturer) or an extended size of 1024 x 1024 pixel
(dark pixels around, which can’t be used!)
17
6.3.3 SENSOR (MISC.) TAB
Pixelclock 1
2
1
3
The pixel clock sets the clock
frequency and therefore the image
sensor readout speed.
Possible settings are 12 MHz (real clock
frequency is 12.5 MHz) or 25 MHz. At 25
MHz the image sensor is read out with
nearly double speed, achieving higher
frame-rates.
The following table shows frame-rates for two active taps.
Resolution
12.5 MHz
25 MHz
1008 x 1008
63 fps
94 fps
800 x 600
106 fps
158 fps
A/D Converter
Not available for pco.flim.
IR Sensitivity
Not available for pco.flim.
Offset Control
Not available for pco.flim.
B/W Noise Filter
Not available for pco.flim.
Conversion Factor 2
The conversion factor defines how many charges (electrons), which
have been generated by light in each pixel, are necessary to generate
one count (one intensity level) in the digital image. Therefore, the
conversion factor describes the gain when converting electrons into a
digital value.
Cooling Setpoint 3
If a pco.flim is connected, sensor temperature is adjustable (air- or
water-cooling). Recommended sensor temperature is 5°C.
Display of sensor temperature: A Peltier cooling unit is used to keep
the sensor's dark current to an acceptable minimum and to allow for
a continuous operation free of any drift phenomena in image
sequences. Either an internal fan or an external water cooling system
assures proper heat transfer from the Peltier element to regulate the
temperature of the cameras.
18
6. CAMWARE 3 SOFTWARE
6.3.4 RECORDING TAB
Recorder Mode 1
1
In Sequence mode the recording stops
when (computer) RAM space is full. In
Ring Buffer mode the camera will stop
only by a stop command.
3
2
Acquire Mode 2
Not available for pco.flim.
Timestamp 3
A time stamp can be placed into the upper left corner of the image. It
can be either put off, binary,binary with text or text. The time
resolution is 1μs.
In binary mode the first 16 pixels will be filled with the time stamp
information (binary code). The numbers are coded in BCD with one
byte per pixel, which means that every pixel can hold 2 digits. If the
pixels have a resolution of more than 8 bits, then the BCD digits are
right bound adjusted and the upper bits are zero. For further
information please refer to the SDK.
In binary and ASCII mode text will be placed into the image replacing
the content of the image (272 x 8 pixels ASCII).
Time step shows the end of exposure time.
Three different information is stamped onto the image: number of
the image 1 , date 2 and time 3 .
1
19
2
3
6.3.5 I/O SIGNALS TAB
1
2
3
4
Exposure Trigger 1
If checked, a signal for External Exp. Start trigger mode (see chapter
6.3.1) is accepted at the Exposure Trigger (see A1.3).
Status Busy 2
A signal indicating busy status is given at the status busy output.
Once an acceptable trigger edge is received, busy will go on status
high. As soon as busy goes low again, a new trigger edge is
accepted. (negative logic possible, too!)
Status Expos 3
A signal indicating exposure status is given at the status output.
Status Expos indicates the actual exposure window for one frame.
Light Enable 2
This connection shows a signal of an external light source.
Filter
electrical interference filters
Polarity
Type
active for high/low signal
or rising/falling edge
Maximum low level: 0.8V
Minimum high level: 2V
Slew Rate >1ms/V
TTL Electrically grounded, no
opt coupler.
3.3Volt LVTTL out , short time
short-circuit-proof.
20
6. CAMWARE 3 SOFTWARE
6.4 CAMWARE FEATURES
This chapter describes in detail the Camware Demo Mode and the
File, Camera, Acquisition, View and Window menus.
6.4.1 DEMO MODE
When Camware is started, it automatically recognizes the
camera type of the connected and running cameras.
Camware will start in Demo Mode, if your camera is switched
off or no camera is connected
In this mode all image processing features are available.
The user only has to tell Camware what type of image he
wants to open. For that purpose, the Demo Mode Setup
window opens and asks for the corresponding input.
Need Help? If this window pops up because you have trouble
running the camera, please see instructions in appendix
A5.4.
Resolution
The drop down list displays the existing image sensor spatial
resolutions of all PCO camera systems. Please select the specific
resolution and bit depth of the images to be opened. If double shutter
images have been recorded and should be opened, Double Shutter
Mode should be checked.
Color
With the radio buttons, the user can specify whether the image type
is monochrome (b/w) or color.
Alignment
These two radio buttons adjust whether MSB (most significant bit)
aligned (upper) or LSB (least significant bit) aligned (lower) images
have been stored (see 6.4.2 File Settings)
Settings to view the b16 files of the pco.flim
Resolution
1008x1008
Bit
14
21
DoubleShutter
no
Color
b/w
Alignment
upper
6.4.2 FILE MENU
NOTE
be aware of the different storage abilities of the
formats, for example *.bmp - the bitmap format only
allows for 8bit values to be stored and therefore the
image content of a 16 bit image is reduced, if stored
as bitmap.
Open RAW File (single image only)
This command should be used to import a single image
into the currently active image window. Only files with the
extension and format of *.b16 (=PCO proprietary binary
image format) and *.tif (16 bit TIFF image format) can be
imported. If the recorder is enabled, each imported image
will be transferred to the buffer shown in the picture
number. The image itself will be fitted to the current
image size. If the recorder is disabled, the current image
sizes will be set to the parameters of the imported image.
Open RAW File Set (only for pco.hsfc pro camera)
Open RAW Recorder Sequence (image sequence from one camera)
This command is used to import a sequence of images. If more than
one camera is connected and an image window is currently open, the
sequence will be loaded to the active window. If no image window is
open, the images will be loaded to camera #1. This command opens
the Open file dialog box. Only files with the extension and the format
of *.b16 and *.tif (TIFF16) can be imported.
Save RAW File (single image only)
This command should be used to save the image, which is displayed
in the active window. The command opens the Save file dialog box.
The image file can be saved in 16bit *.b16 and *.tif format. If more
than one camera is connected, it is possible to save all current
images by selecting Export all images in the Save file dialog box.
With this feature it is possible to save one image of each active
camera within one process step (it is not necessary to repeat the
save process for each camera). The Save command will not be
available, if no image window is open.
Save RAW Set (only for pco.hsfc pro camera)
Save RAW Recorder Sequence (image sequence of one camera)
The Save Recorder command should be used to save or export
image sequences. If more than one camera is connected and an
image window is currently open, the record of the active window will
be saved. The command opens the Save recorder file dialog box. The
Save Recorder command will not be available, if no image window is
open.
Export File (not reloadable!)
Use this command to export the image of the active image window.
This command will open the Export Image dialog box. Files with the
extensions fts, tif, bmp, asc, jpg, and jp2 can be exported. This item
will not be visible, if no image window is open
22
6. CAMWARE 3 SOFTWARE
Export Recorder Sequence (not reloadable!)
Use this command to export a sequence of images. If more
than one channel is connected and an image window is
currently open, the record of the window which has got the
input focus will be saved. If no image window is open the
Export Recorder Sequence menu does not appear. This
command will open the Export recorder box. Files with the
extensions fts, tif, bmp, asc, avi, mpg, jpg, jp2, and wmv can be
exported. (see Appendix A4)
Print Setup
The Print Setup command opens the Windows Print Setup dialog box
for adjustments and settings of the connected printer(s).
Print Preview
The Print Preview command opens a Print Preview window.
Print
Opens the Windows Print dialog box.
This command opens the Options dialog, allowing special settings.
File Settings
Tiff File Settings: Align to MSB
Here you can select the alignment of the pixel
value, if the pixel depth is less than 16bit (e.g.
MSB: each pixel value is shifted from bit 12-1 to bit
16-5 in case of 12bit). This removes the dark image
effect inside other image applications.
Tiff File Settings: RAW 16bit RGB
Here you can select whether the 16bit RGB tiff file
is saved with 16bit raw or white balanced data. If
this item is checked, each 16bit RGB pixel value is
saved as raw 16bit RGB (48bit) value, without
white balancing. Saving to 16bit RGB can be done
by selecting the color display window, export
recorder, selecting 8bit-tiff and checking Save
16bit RGB.
ASCII file separator
Here you can select an appropriate separator for the ASCII file
format.
JPEG File Settings
Here you can select the quality of the saved jpeg/jp2 files: high
quality saves images with 100% quality (otherwise 85% quality).
Preserve Last Record
If checked, a message box will pop up in case the user may overwrite
or loose data.
23
Use six digits for filename
If you record a sequence of *.b16 files and choose direct record to file
Camware is always using six digits for filename (e.g. t_123456.b16).
View Settings
Image Browser
Here you can select between showing all image
tiles inside the browser window either b/w or
color. Setting b/w will result in a faster refresh,
(valid only for color sensor).
Convert (Min/Max)
(Continuous Auto Range Crop, see chapter 6.4.5
Convert Control BW) This option enables the
automatic min/max function during record and
replay. Disable this feature if the application stops
responding during this mode (due to operating
system capacity problems).
Marker
Use this command to display a crosshair in the center. It is possible
to adjust length and color of the crosshair.
Paint Images
This option enables the update of (both double shutter images or)
multiple cameras images during record and replay. Disable this
feature if the application stops responding during this mode (due to
operating system performance problems).
Toolbar
Toolbar
Opens the corresponding toolbars (see 6.2)
Math. Functions
With the math window you have the ability to build
the difference between a reference image and the
actual image. If you activate math every new image
acquired will be subtracted from the reference
image or vice versa.
last acquired image will be copied to reference
buffer
a reference picture will be acquired and copied
to reference buffer
add offset to avoid negative values, which would not be visible
enable / disable math function
Replay Speed Bar
The faster replay speed is useful if large sets of image data need to
be scanned.
24
6. CAMWARE 3 SOFTWARE
Misc
Infotext
Use this command to show or hide the start-up
information dialog box.
Synchronous Scrolling
If this item is checked all opened image windows
will be scrolled at the same time and in the same
manner.
View Embedded Image Info (not available)
Multi Win Picture Order
With the Multi Window you have the ability to view
the images of all active cameras in one window.
You can select between camera order oriented or image time
oriented order. Image time oriented means that the sequence of
images depends on the individual time stamp of each image.
Camera order oriented means that the first image shown is from
camera 1, then camera 2 and so on.
Logfile
This option enables the logging of errors and system info. Enable this
feature in order to create a support file, which can help to find out
troubles with the camera (see A4 )
Load lookup Table (for monochrome cameras)
With the Pseudo LUT (Lookup-Table) feature you can load any LUT
with one of four different formats and you can view the result in the
color view window. Use one of the attached predefined LUTs or
define your own.
Direct Record to File
This command is intended for cameras with internal memory. With
this command you can preset a certain number of images to be
stored. If the camera captures images faster than the computer can
save to disk, then you will lose images. The displaying of the images
doesn't interfere with the record process.
Record with Flowfile
This command should be used to load a flow file. A flow file contains
lines with a delay and an exposure time. Each line corresponds to
one image to be acquired. If the flow file has 15 delay and exposure
times than 15 images will be acquired. Before the image is snapped,
the corresponding time values will be sent to the camera. This affects
the possible image frame-rate, as additional time is required for
setting the values between two images. Please see the sample flow
files in the application directory (e.g. flow.txt).
Exit
This command exits the program and closes all channel dialog
windows. Window positions, settings and sizes are stored in the
windows registry and will be loaded again at next start-up.
25
6.4.3 CAMERA MENU
Camera Control
Use this command to open the camera control window (see 6.3).
Load Camera Settings
With this command you can reload camera settings which have been
previously saved. You can reload only a file which has been made
with the same camera configuration as it was found by the
application at start-up.
Save Camera Settings
This command saves the actual camera settings of all connected
cameras.
NOTE
Some pixels tend to
become very bright
during a long exposure.
These pixels are called
hot pixels.
Create Hot Pixel List (only for long exposure times– not available)
Use this command to create a Hotpixel-File. To remove hot pixels
from the image you have to create a hot pixel file first. If you have
already a hot pixel file you only have to enable hot pixel correction.
The new value of the hot pixel will be calculated from the values of
the neighbouring pixels. You only can create a hot pixel-file if the last
exposure was longer than 5s. This command will open the hot pixel
dialog. You have to type in the desired count of hot pixels first. Then
press the Get Hotpixel button. After this the hot pixel list will be
created, using the last acquired image. Now you have the possibility
to save the hot pixel list for later use. All list box items with a
checkmark will be saved to the hot pixel file. You can select or
deselect a group of items by highlighting a group and pressing the
checkmark in front of one of the highlighted items. Additionally you
can open a local menu inside the list box by pressing the right mouse
button. You can verify your selection by scrolling through the list and
by a comparison to the average value shown in the dialog.
Hotpixel Correction (only for long exposure times– not available)
You can enable or disable the hot pixel correction. If hot pixel
correction is disabled, a file dialog will be opened, in which you have
to select the correct hot pixel file for the corresponding camera. If you
have more than one camera connected you have to select the correct
camera number first. If hot pixel correction is enabled this feature will
be switched off.
Auto Exposure
Starts some exposures and tries to find an exposure time that
captures images with grey values between 1400 and 1500. It readjusts the lookup tables in order to display the images with the new
settings best. For low light conditions only.
Close
Disconnects camera and switches Camware to Demo Mode. In case
of multiple cameras, all cameras must be closed in order for
Camware to switch to Demo Mode.
Rescan Disconnect and reconnect camera.
Setup (not available for pco.flim)
26
6. CAMWARE 3 SOFTWARE
6.4.4 ACQUISITION MENU
Live Preview
The Live Preview is useful for fast and easy adjusting and
focusing of the camera. The active window will be updated. To
see another window, simply click on the window. This option is
not available in double shutter mode.
Acquire Picture (not available)
Records single images in case of sensicam cameras.
Acquire Sequence
Starts recording images into the system memory according to
Trigger Mode selection (see 6.3.1). During the recording, the
camera controls are locked.
Rec. Memory Settings
This sets the number of images recorded in one sequence. The
maximum is defined by approved RAM size.
Recorder Settings
This command opens the Recorder Settings dialog window.
The Play Settings are referred to the camera. If more than one
camera is connected, the camera number is retrieved from the image
window that has received the input focus (is active). If no image
window is opened, the dialog is referred to camera 1. If the Set to all
checkbox is checked the settings apply to all connected cameras.
The Global Settings are valid for all connected cameras.
Averaging
If in the Avg. dropdown list a value not equal to 1 is selected, the
corresponding number of images is averaged in the buffer, reducing
the statistically independent noise.
Another option to reduce the noise is the activation of the
Infinite impulse response IIR lowpass filter. This filter takes 90% of
the previous image and 10% of the new image to create images with
clearly reduced noise. Image (actual) = Image (act - 1) * 0.9 + Image
(new) * 0.1
Replay Speed Slider
The slider can be used to adjust the sequence replay speed, from
slow (turtle) to fast (rabbit).
Seq. Wrap Rec
Sequential Wrap Recording sets Recorder Mode to Ring Buffer. If
disabled Sequence is active (see 6.3.4).
Seq. Wrap Play
The play button starts the display for a sequence as defined by the
first image/last image fields and will loop until the stop button is
pressed.
Reverse Play
If checked, it is possible to play the recorded images in reverse order.
27
6.4.5 VIEW MENU
B/W Window
Use this command to open a b/w display window.
Color Window (Color version not available for pco.flim)
Use this command to open a color display window.
Convert Control BW
The user can influence how the 14 bit intensity values (x-axis) of the
original image are converted into 8 bit values (y-axis) in different
ways.
BW Settings (includes histogram of original
data)
It is possible to hide the histogram of original
data 1 and to switch tab/histogram 2 .
4
1
Green sliders in histogram 3
left slider = Min controller (corresponds to
value 0 of the 8 bit display). Values below that
mark are set to 0, i.e. displayed as black.
right slider = Max controller (corresponds to
value 255). Values above that mark are set to
255, i.e. displayed as white.
2
3
The values in-between are converted into a value between 0 and 255
according to Contrast and Gamma settings. See the small graph 4 ,
which reflects the calculation.
Converted Hist
This tab shows
converted data.
you
the
histogram
of
How the Auto Range applies
Auto Range Peak searches for the minimum and maximum 14 bit
intensity values of the image. Given these number the converter
scales the 8 bit display (256) within these two values.
Auto Range Crop sets the converter to ignore the extreme intensity
values of the image and scales the display in a smaller range. Thus
dark or bright light spots, reflections, etc. are cut off.
Min/Max (Automatic) = Continuous Auto Range Crop
Proc config tab: please see under Convert Control Color
28
6. CAMWARE 3 SOFTWARE
Proc. Config (Process configuration)
Due to proprietary high-end algorithms used for these image
processing features, no detailed description is given here
1
2
1 Switch on in order to significantly reduce
processing time
2 Pixel color correction
3 Non local means algorithm
4 Adaptive to brightness and patterns
5 Sharpen
3
4
5
Convert Control Color (not available for pco.flim)
GPU Processing
In order to benefit from all of Camware’s advanced processing
features, we highly recommend a NVIDIA CUDA-enabled graphics
processing unit (GPU). AMD's ATI graphics processing units are not
recommended.
Find a list of CUDA-enabled products here:
• https://developer.nvidia.com/cuda-gpus
Get the CUDA driver here:
• https://developer.nvidia.com/cuda-downloads
Make sure:
• that the CUDA driver is successfully installed.
• that the GPU has at least 512MB memory.
Browser
The Recorder Browser gives an overview of the images in the
Recorder, and helps you in an easy way to select a new current
image. Simply click on the desired image to make it the current
image. It may then be viewed in the Black/White and Color Window.
The Browser window can show up to 40 pictures per page, thus
enables an easy overview of the recorded images. You can switch
between the single pages by double clicking inside the Browser
window or with the local menu (right mouse button).
Skip #images allows for convenient overview at large numbers of
images.
Multi Window
View the images of all active cameras in consecutive order in one
window. Time oriented means that the sequence of images depends
on the individual time stamp of each image. Camera number oriented
means that the first image shown is from camera 1, then camera 2
and so on.
Embedded Image Info (not available for pco.flim)
29
6.4.6 WINDOW MENU
Cascade
Show all windows overlapped.
Tile
Tile windows vertically or horizontally.
Arrange Icons
Rearranges all minimized topic icons on the desktop.
Close All
Close all windows.
6.4.7 HELP MENU
Contents
Opens the main page of the program help.
Search for Help on
Opens an index list for help.
About Camware
This window shows program information and contains
helpful support links. In case of a camera problem,
support files (see 6.4.2 Options/Misc/Logfile) may be
generated, which can be automatically attached to the
email. The support files contain relevant hardware
information, the log files and the pco related registry
entries. These files are moved into a zip-archive called
CWSupport.zip, which can be found in the application
folder.
30
6. CAMWARE 3 SOFTWARE
6.4.8 LOCAL MENU

The local menu is opened by clicking the right mouse button inside
the client area of an image window.
Image Properties
Opens an information bubble with main image properties and
activates the in-image display. It does not overwrite image data.
Camera Control
Opens the Camera Control window (see chapter 6.2).
Convert Control BW (Color version not available)
Opens the corresponding Convert Control windows (see 6.4.5).
Zoom
Changes the zoom used to display the image.
Auto Range Peak/Crop
Displays the image in a predefined scaling (see chapter 6.4.5).
Auto Balance Color (not available for pco.flim)
The white balance feature, which corresponds to the white balance
button in the Convert Control Color (see chapter 6.4.5).
Auto Exposure
Auto Exposure feature (see chapter 6.4.3)
Flip
The image will be flipped before display.
Mirror
The selected image will be mirrored before display.
Rotate
The image will be rotated by 90° counter clockwise (left) or clockwise
(right) before it is shown in the image window.
Line Tool
Select Line Tool to show a graph, which
shows the intensity of the pixels under the red
line shown in the image window. The selection
of this menu item determines the location of
the first point. After this you can move the
mouse to the second point, which can be
selected by a left click. Now a Line Diagram opens and the length
of the line (unit: pixel) is shown in a bubble and displayed in the
window.
Copy To Clipboard
Copies the active image to clipboard (only as 8 bit image file).
.
31
6.4.9 ADDITIONAL FEATURES
White Balance by Mouse
In the same manner you can change the white balance. You only
have to press the CTRL (Strg) and the shift button at the same time
and select a white or gray area within the image. The pixel values
within the coordinates of the rubber band window are used for
calculating a new white balance.
For best results we recommend to use the white balance button in
the Convert Control Color (see 6.4.5) or the Auto Balance Color in the
Local Menu (see 6.4.8) respectively.
Fold Up Window
1
The Convert Control windows can be
minimized / folded up 1. Just move the pointer
over the bar and the window will unfold
again 2 .
2
Setting Contrast Area by Mouse
You can control the minimum and maximum values used for the
conversion from 14 bit to 8 bit with the mouse. Move the mouse
cursor into a region which should be shown with maximum contrast.
Press the shift and the left mouse button. Hold down the mouse
button while increasing the size of the rubber band window with
mouse moves. After releasing the mouse button the coordinates of
the rubber band window act as a border for calculating the minimum
and maximum values.
Setting a new ROI by Mouse
In the same manner you can setup a new region of interest (see ROI
6.3.2) for the camera. You only have to press the CTRL (Strg) button
and drag an area with the left mouse button instead of the shift
button. The coordinates of the rubber band window are used for
calculating a new region of interest, which will be adapted to the
camera capabiliies automatically. You can reset the ROI to maximum
by pressing the CTRL (Strg) button and the right mouse button.
32
7. [email protected] SOFTWARE
7. [email protected] SOFTWARE
The [email protected] software is a tool for image recording and analysis
with a pco.flim camera system.
Please install the [email protected] software from the PCO usb flash drive
or download the latest version from Fish’n’Chips website.
7.1 START
When double-clicked on the program icon the program starts with
this start window, where the following main menu topics are
present.
The menu tabs are: Data Files (see 7.2) – Camera (see 7.3) and
Licensing (see 7.5).
33
7.2 DATA FILES TAB
The Data Files Menu processes stored image data sequences. It
distinguishes between reference data, which can be loaded with the
Load Reference button and measured data, which can be loaded
with the Load Data button. Depending on the selection, additional
information is required. Usually, before measured data can be
evaluated, a reference data set should be loaded.
7.2.1 LOAD REFERENCE
The main window has three operation areas: menu bar 1 , the control
window 2 and file / data tree window 3 .
1
3
2
The main window shows the calculated intensity image in a
normalized way, such that the value range is 0.0 – 1.0. Within the
menu area some new fields appeared after loading the reference data
file.
Menu Bar 1
Now, next to the Load Reference button,
a Remove Reference button appears. It
removes the reference data from the
program memory. The filename of the
currently loaded reference file is also
shown. Below the filename there is a checkbox for asymmetry
correction. Here, the user has to tell the software whether the
reference image sequence contains a second sequence for
asymmetry correction. The default value of the checkbox is checked,
so the software expects sequences containing additional images for
asymmetry correction.
Further, for the conversion of phase angle and modulation index
values into valid fluorescence lifetime distributions, it is necessary to
tell the software which modulation frequency [fmod] has been used and
which is the known lifetime [τref] of the reference luminophore. The
values can be typed in and the drop-down list beside the number
fields allows to select the appropriate unit.
34
7. [email protected] SOFTWARE
Control Window 2
The control window (column) allows for the adjustment of the
processing and gives results and information of the active image.
Lower / Upper Noise Level: On top there are two number fields
which define so-called noise levels. Before the images are processed,
the intensity image is pixel-wise compared with these levels, and
pixels which are out of the given range (value is smaller than the
lower noise level or larger than the upper noise level) are ignored
and not used for calculation and processing. In the result images this
is indicated by setting the pixel values to black or zero. Since the
levels refer to the normalized intensity image, the range is always
from 0.0 – 1.0.
Cursor Size: The drop-down list right to the cursor size designator
allows the selection of single pixel readout or the average value of a
field of (3 x 3), (9 x 9) or (21 x 21) pixels.
Min / Max Value: Below the noise levels there are two number fields
which are named min and max with a push button with four arrows
between them. These values scale the display of the image pixel
values according to the value range of the active image (for intensity
image from 0.0 – 1.0, for raw data from 0 – 16383, for lifetime
distribution from 0.0 – x in [s] etc.). The settings change from active
image to active image. The push button performs a sophisticated
auto scaling which can lead to undesired behavior in case the noise
levels were not properly chosen.
Histogram with scaled grey or color wedge and cursor readout:
The histogram below the minimum / maximum values shows the
distribution of the values of the active image within the defined
minimum / maximum range. Below the histogram the corresponding
grey or color wedge is displayed, which is like a horizontal grey or
color bar. The line, which points down to further values, is the
readout of the cursor, located in the active image. The values given
below the histogram correspond to the x–y–coordinates of the cursor
in the active image, and the bold value is either the cursor readout or
the average of the selected area around the cursor, which is defined
by the cursor size selection. It can be a single pixel value or the
average of a field of (3 x 3), (9 x 9) or (21 x 21) pixels. The standard
deviation is given as σ value.
File Tree Window 3
In this window (column) all loaded image files, processed images and
live images are listed and can be selected. The selected image is
displayed as active image in the central window, and the control and
readout values from the control column relate to this active image.
Each of the raw images can be selected as well as the calculated and
referenced images, if exist, and can be made active for display.
First the filename is displayed or Live if it is a live image. Then all the
raw images of the sequence can be selected if the list is unfolded by
clicking on the arrow.
35
Below the raw images the relevant calculated images are listed:
Intensity – Modulation (index) distribution – Phase (angle) distribution.
-1
-2
-3
-4
Raw Images
Intensity
Modulation Index
Phase Angle
1
2
3
4
36
7. [email protected] SOFTWARE
7.2.2 LOAD DATA
If a data file is loaded some elements are added to the Menu bar.
Menu Bar Add Ons
As in the case that reference data is
loaded, the filename of the measured
data is displayed and a checkbox is
added to tell the software whether the
data images have been recorded with a
second
sequence
for
asymmetry
correction or not. The default value of the
checkbox is checked, meaning that the software initially expects
sequences with images for asymmetry correction. Further, an
additional button has appeared for the removal of the data from the
program memory.
File Tree Window
Now, the files or images from the Load Data event are shown in the
file tree window. The raw images can be selected, as well as the
calculated images, as for the reference data, and a new section
appears called Results.
In the Results the calculated and referenced images are presented.
This means that the modulation index and the phase angle
distribution images have now referenced values, which can be
converted into luminescence lifetime distributions each. Further, the
modulation index as the magnitude of a vector or phasor and the
phase angle as its angle can be displayed in one plot, the phasor
plot, which is a type of Nyquist plot.
Modulation Lifetime
This is the luminescence lifetime
distribution derived from the referenced
distribution of the modulation indices
per pixel.
37
Phase Lifetime
This is the luminescence lifetime
distribution derived from the referenced
distribution of the phase angle per pixel.
Phasor Plot
This is a Nyquist plot where each pixel
represents a vector with a magnitude
given by the referenced modulation index
and an angle towards the x-axis given by
the referenced phase angle.
38
7. [email protected] SOFTWARE
7.2.3 EXPORT RESULTS
When the Export Results button is pressed, the following
dialog window pops up:
The Folder Button allows selecting a different directory for
storing the results with an additional file manager dialog. In case
the calculations have been repeated with another reference file,
and the old results are not required any longer, it is possible to
check the checkbox overwrite existing files without notice,
such that the old files with the same filename are overwritten.
The suggested base filename for the result files is the original
data filename without extension. It can be edited and changed
by an input into the text window beside base filename.
The file tree window shows all calculated images:
Reference: Intensity – Modulation – Phase
Data: Intensity – Modulation – Phase
Results: Modulation – Phase – Modulation Lifetime – Phase
Lifetime – Phasor Plot
If the higher level checkbox is checked, the lower level files are
all checked automatically. All checked files/images will be
stored as TIFF-Files containing floating numbers, so they
become images with non-typical image content. Software like Matlab
and ImageJ can process these value distributions. The filenames are
created as the combination of the base filename plus the
corresponding name in the file tree window.
39
7.3 CAMERA MENU
When the program is started with a
camera connected and running, the
[email protected] program opens with this
window with the Camera Menu selected.
7.3.1 MENU BAR
The menu bar has the following elements:
7.3.2 FIND CAMERA
The magnifying glass button is helpful, in case the camera is
switched on after the program has been started or if for some reason
the camera has been switched off or disconnected while the program
is running. If pressed, the program scans for connected and available
cameras.
In case the program has found a camera, next to the magnifying
glass a text describes which camera is connected via which interface
and the serial number (which is relevant for the licensing). In this
case: a pco.flim connected to USB 3.0 serial 1090013.
Next to the right, two gear wheel buttons follow, which serve to
unfold different configuration tabs.
40
7. [email protected] SOFTWARE
7.3.3 SEQUENCE CONFIGURATION TAB
1
NOTE
Relative Phase:
This feature is only
available in the special
case the phase number
(see below) has been
set to manual and the
camera is in recording
state.
Here all control settings
for the measurement of
3
2
Frequency
Domain FLIM
can be made.
There are three sub sections presented: Modulation – Phase
Sequence – Image Processing.
Modulation 1
Here the relevant settings for the internal direct digital synthesizer,
the modulation signal generation, can be made.
Source Select: The dropdown list offers either an “internal”
modulation signal generation or an external modulation signal input.
Output Waveform: The dropdown list offers none for no signal
output, sinusoidal for a sinusoidal output signal or rectangular for a
rectangular output signal shape.
Master Frequency: The input window allows for the numerical input
(as integer) of the desired modulation frequency in the range from
5 kHz to 40 MHz, while the dropdown list allows for the selection of
the corresponding frequency unit: Hz, kHz and MHz.
Relative Phase: This allows the manual control of the phase angle
shift between image sensor modulation signal and the modulation
output signal to an external device. The values should be in degrees.
Phase Sequence 2
Phase Number: The dropdown list enables the selection of the
number of phase angles per modulation period (360°), which shall be
measured in a data sequence for the reconstruction of the sinusoidal
or higher harmonic signal, which is detected: 2 – 4 – 8 – 16.
Phase Symmetry: The dropdown list allows the selection of singular
or twice. This is the main selection if a single sequence (singular)
shall be recorded or a second sequence (twice) is appended with
opposite phase angles in the taps A and B, if the asymmetry
correction should be performed by either the camera or the PC.
Phase Order: This selection is only relevant if the Phase Symmetry is
set to twice. It defines if the inverse (opposite) measurements are
made either as consecutive reverse sequence, such that a first
normal sequence is recorded and then the reverse (appended)
sequence, or as consecutive revers pair, which means that the
reverse measurement is made directly after the corresponding dual
tap read out image.
Tap Select: This dropdown list selects whether tap A, tap B or both
taps are readout.
Image Processing 3
Asymmetry Correction: Here it can be selected if the camera shall
perform the asymmetry correction internally. If off is selected from
the dropdown list, the camera does not perform the asymmetry
correction internally. One has to take care, that the asymmetry
correction checkbox in the Load Data Section of the [email protected]
program is unchecked, when loading data recorded and saved with
the internal correction option set to on, since the correction has been
already made by the camera.
41
7.3.4 EXPOSURE TIME / NUMBER OF IMAGES
Exposure time [ms]: With the numerical input into this window, the
exposure time for a double (both taps) or a single (tap A or tap B)
image is set in milliseconds.
Number of images: displays the number auf recorded images; can
bet set by the Sequence Configuration Tab 7.3.3
7.3.5 FUNCTION BUTTONS
Record Sequence Button
1
If pressed 1 one complete data sequence
is recorded by the camera using the
corresponding
settings
from
the
configuration tabs. The image data are
directly displayed, and if a reference data set has been loaded or
recorded prior to that, the result images are calculated as well.
Additionally the total number of recorded images is displayed to the
left of this action button.
3
All the recorded and calculated images are listed in the File Tree
Window on the left.
Since the recorded data have not been saved yet, they are named as
Live data with the normal names of calculated images and result
images. By selecting the desired image 3 in the file tree, this image
is made active and displayed in the main window.
4
While recording a small preview window 4 is shown in the left
column.
42
7. [email protected] SOFTWARE
Preview Image Toggle Button
If pressed, it sets the camera back to a
continuous dual tap but single image
recording at the defined exposure time.
The button will be highlighted to indicate,
that it has been turned on. During
operation the live intensity image is always updated. This mode is
useful for exposure time optimization and focusing of lenses or
microscopes. When pushed again, it is deactivated and the original
sequence configuration settings are loaded back to the camera.
When the preview image toggle button is
pressed, the live intensity image is
updated
continuously
to
facilitate
adjustments of the optical setup or to
figure out the optimum exposure time.
Since the shortest possible sequence
length is used here, this mode is well
suited for focusing purposes.
Save as Data Push Button
If pressed, a save file dialog pops up, and
the user can store the recorded image
sequence as data.
43
Save as Reference Push Button
If pressed, a save file dialog pops up, and
the user can store the recorded image
sequence as reference data.
Thereupon,
the
reference
image
sequence appears in the File Tree
Window on the left side with the defined
filename. It is organized using the listing
described above.
NOTE
Now the reference data set is used for referencing the following
measurements and calculating the results. In order to obtain valid
lifetime information the user has to define the known lifetime of
the reference by switching to the Data Files menu and typing in
the lifetime with the appropriate time unit.
44
7. [email protected] SOFTWARE
7.4 VIEW MENU
2
1
3
The View menu allows the user to control and select the
displayed images and corresponding information
windows ranging from single to multiple image display.
The zoom level can be set to Original Size or the whole
image can be fitted to the display window (Fit to
Window). The menu bar has the following options.
Image orientation: 3 selectable orientation - Up (standard) – Right –
Down - Left
Modes
There are two display modes available, Single
Image and Multiple Images 1 . If Single Image is
selected, there is one image displayed at a time
(either in Original Size or Fit to Window zoom
stage 2 ).
5
The image selection is activated by clicking on the
appropriate image name in the File Tree Window on the
left side. 4
The selected image is highlighted by a light grey
background behind the name. On the right side the
Control Column 54 can be used to adjust the scaling of
the image, control cursor size and read values at the
current cursor position.
4
45
Effect of the Noise Levels in the Images
The left image shows the phase angle distribution of a bright data
image with the lower noise level set to 0 and the high noise level
set to 1, which means everything is visible. The right image shows
the effect of setting the lower noise level to 0.05 (it is in units of the
normalized intensity image: 0.0 – 1.0). Most of the surrounding noise
of the fluorescing cell is now suppressed.
If the signal is too bright, i.e. the mean intensity becomes more than
approximately 0.5, clipping will appear. Although the image itself is
not overexposed, derived values from bright parts might already be
clipped, such that modulation index and phase angle start to distort.
If now the upper noise level is set to 0.5, the parts of the image
exhibiting a too large signal are suppressed. This can be seen by
means of the black area within the sample, indicating that the
significantly different phase angle in this area, which is reddish in the
left image, is more an artefact.
46
7. [email protected] SOFTWARE
Multiple Images
In case Multiple Images has been
selected, several images can be
displayed in the image area at once (in
the example with the Original Size zoom
factor 5 ).
5
Now in the File Tree column checkboxes appear to the left of each
image, such that the user can select those images, which should be
displayed 6 .
Depending on the total number of selected images the available
space in the image area is subdivided to display all the images
(maximum of eight images), such that the maximum space is used for
each image.
6
The following image shows an example
with six images displayed simultaneously.
47
Using the Multiple Windows selection, each of the selected images
has a corresponding histogram with a scale bar for scale adjustments
in the Control Column on the right side, where all correspondences
can be identified by the titles of the images and the histograms 7 .
7
The crosshair of the cursor in the current image is displayed in white
color 8 while the corresponding position in all other images is
displayed in black 9 . Nevertheless in all images (except the phasor
plot) the values at the same cursor positions are read and displayed
below the corresponding histograms.
9
8
9
8
48
7. [email protected] SOFTWARE
Zoom
Two different zoom factors are available: the Original Size which
corresponds to a pixel by pixel representation of the measured image
as can be seen for Single- and Multiple- windows:
Or the images are fitted to the available image space, when Fit to
Window has been selected, as it is shown for Single and Multiple
windows:
49
7.5 LICENSING MENU
The last menu is the Licensing menu, which informs the user which
license for [email protected] has been activated and offers the possibility
to request or to import a license file in case the computer has no web
access.
1
Active License
The text field gives information about the name of the
user and his affiliation, type of license (per PC or per
camera) and expiration date, if exists 1 .
Request License
If the Request License Button 22 is pressed, the
following window pops up 33 .
3
The program gathers required information about
the computer and sends it to the company
Fish’n-Chips, the originator of the [email protected]
software. With this information a license file is
created and uploaded to the computer.
In case there is no access to the internet a text
file with the required computer or camera
information is created and the user is asked to
send it to a provided e-mail address to receive
the license file via e-mail.
2
Import License File
In case the user has received a license
file, it has to be imported. By pressing
the Import License File button a file
manager dialog opens. Here the user
has to switch to the directory where
the license file has been stored. By
selecting the license file and pressing
open (öffnen) 4 the license will be
directly processed by the program.
4
50
APPENDIX
A1 TECHNICAL DATA
52
A1.1 MECHANICAL DIMENSIONS
52
A1.2 DATA SHEET
53
A1.3 REVERSE OF THE PCO.FLIM
54
A2 USB 3.0
56
A2.1 HARDWARE RECOMMENDATIONS
56
A2.2 INSTALLATION OF THE USB 3.0 CARD
58
A3 WATER COOLING PCO.AQUAMATIC II
59
A3.1 SYSTEM COMPONENTS
59
A3.2 FIRST TIME INSTALLATION
60
A3.3 OPERATION
61
A3.4 DIMENSIONS
62
A4 IMAGE FILE FORMATS
63
A5 CUSTOMER SERVICE
65
A5.1 SERVICE
65
A5.2 MAINTENANCE
65
A5.3 RECYCLING
65
A5.4 TROUBLE SHOOTING
66
A6 INDEX
67
ABOUT PCO
68
51
A1 TECHNICAL DATA
A1.1 MECHANICAL DIMENSIONS
pco.flim watercooled version
All dimensions given in millimeter.
Input Window Filter
The input window of the pco.flim camera contains a standard coating
(providing better light transmission).
52
A1 TECHNICAL DATA
A1.2 DATA SHEET
image sensor
type of sensor
image sensor
resolution (h x v)
pixel size (h x v)
sensor format / diagonal
shutter mode
fullwell capacity
readout noise
dynamic range
quantum efficiency
spectral range
dark current
DSNU
PRNU
camera
max. frame rate
(full frame, full resolution)
modulation frequency
CMOS
proprietary
1008 x 1008
5.6 µm x 5.6 µm
5.7 mm x 5.7 mm / 8.1 mm
rolling reset / global exposure
45 000 e- (typical)
45e- rms (typical)
> 1000:1 (60 dB)
appr. 39 % @ peak
visible (tbd)
1100 e- / (s.pixel)
49 e- rms
0.7 %
90 fps (2 tap readout)
trigger output signals
modulation signal output
modulation signal input
data interface
internal 5 kHz – 40 MHz
external 500 kHz – 40 MHz
sinusoidal / rectangular
10 ns – 10 s
14 bit
3.2 e- count
steps of 16 x 1 pixel
+ 5 °C
<1%
exposure start
(phase sequence trigger)
exposure, busy, gate (light enable)
1 V peak peak in 50 Ω , AC coupled
max. ± 5V in > 1 k Ω
USB 3.0
general
power supply
power consumption
weight
ambient temperature
operation humidity range
storage temperature range
optical interface
CE / FCC certified
90 – 260 VAC (12 VDC optional)
40 W max.
2.4 kg
+ 5 °C to + 40 °C
10 % - 90 % (non-condensing)
- 20 °C to +70
C-mount
yes
modulation signal shape
exposure / shutter time
dynamic range A/D
A/D conversion factor
region of interest (ROI)
thermoelectrical cooled
nonlinearity
trigger input signals
53
A1.3 REVERSE OF THE PCO.FLIM
Power Input 12V 1
5
Camera Link A & B Connectors (not available) 2
4
7
1
USB 3.0 connector 3
3
6
Power switch (on/off) 4
2
Status LEDs 5
If you turn on your camera 3rd LED (yellow) will light
up. After about 10 s 1st LED (green) will light up and
the orange one will disappear, that means: camera
status ready. After start-up of software (camera
recieves settings from Camware) 2nd LED (orange) will light up.
While recording is in progress 2nd LED (orange) flashes
permanently. If an error occurs, a red LED lights up permanently.
The Trigger connector 6 is a Hirose HR10-7R-6S (73).
Pin Assignment
Pin
1
2
3
4
5
6
Description
Not used
Trigger
Not used
Exposure
Busy
Ground
Pin Position
type
level
coupling
current
6
Inputs (Trigger)
digital
3.3. V LVTTL (5V tolerant)
DC
1kOhm
54
1
Outputs (Exposure, Busy)
digital
3.3 V LVTTL
DC
Max. 100mA (short-term)
A1 TECHNICAL DATA
BNC Connectors: 7
BNC Connector 1:
Modulation Output
type
frequency
amplitude
coupling
impedance
waveform
analog
5 kHz-40 MHz
1Vp-p (in 50 Ohm)
AC
50 Ohm
sinusiodal, rectangular, off
BNC Connector 2:
Modulation Input
type
frequency
amplitude
coupling
Impedance
waveform
analog
5 kHz-40 MHz
Max +- 5V
AC
> 1 kOhm
sinusiodal, rectangular, off
BNC Connector 3:
Light Source Enable (Gate)
type
level
coupling
current
digital
3.3 V TTL
DC
Max 100 mA (short-term)
Timing & Signals
busy = TRUE:
busy = FALSE:
exposure = TRUE:
camera is recording or sensor readout
in progress
external trigger is ready to accept
exposure of image sensor
In Auto Sequence trigger mode the busy signal is always set to
TRUE, while the exposure signal indicates the real exposure of the
image sensor.
In External Exp. Start trigger mode the camera is ready to accept a
new trigger signal on PIN #2 (trigger) when the busy signal is set to
FALSE. After a successful trigger event the busy signal stays TRUE
until the exposure is finished and the image is completely read out.
55
A2 USB 3.0
A2.1 HARDWARE RECOMMENDATIONS
The pco USB 3.0 interface is based on the Cypress EZ-USB FX31
device and it is compatible to pco software such as Camware and
SDK. To run a pco USB 3.0 camera successfully the user should
consider a number of important issues that are discussed in the
following chapters.
Motherboard and Chipset Configuration
It is recommended to use a Motherboard with a state of the art USB
3.0 host controller for the onboard USB 3.0 ports. The latest
generation of USB 3.0 xHCI host controllers manufactured by Intel,
Renesas (NEC), Fresco, Via Labs, ASMedia and Texas Instruments
Inc. (TI) are tested by Cypress Semiconductor Corporation. (see
Cypress EZ-USB® FX3TM SDK Release Notes, Version 1.2.3,
chapter 1.3). Currently pco recommends only motherboards with the
following onboard xHCI host controllers:
• Renesas/NEC μPD720202 Host Controller
• Intel® USB 3.0 eXtensible Host Controller
If the motherboard does not have an onboard USB 3.0 port or the
onboard USB 3.0 port does not work properly with a pco USB 3.0
camera, please use an independent PCIe USB 3.0 extension card.
In this case, pco recommends the following:
• DELOCK 89348 (U3-PCIE1XG202-10)
PCIe 1x (μPD720202 host controller)
(This board is sent with your pco.flim USB 3.0 camera system as standard)
• DELOCK 89325 (U3X4-PCIE4XE101)
PCIe 4x (μPD720202 host controller)
USB 3.0 extension card with xHCI4 host controller
The following companies manufacture USB 3.0 xHCI host controller:
• Renesas Electronics America Inc. (earlier NEC),
• Texas Instrument Inc.,
• VIA Labs Inc (Diamond),
• Fresco Logic Inc.,
• Intel Inc.,
• AMD Inc. and
• Etron Technology, Inc.
NOTE
To determine suitable manufacturers of the xHCI controller,
please use the Windows Device Manager. For example, in
Windows 7 drop down the USB Controller entry and find the USB
3.0 host xHCI controller of the USB 3.0 device in the sub-tree.
Then right-click to open the properties dialog, click the details
tab, and choose Vendor in the properties pull-down box.
56
A2 USB 3.0
The following table gives an overview about tested
recommended system configurations or hardware components.
and
Component
Recommendation
Motherboard
There is no recommendation for a manufacturer. But if an
onboard USB 3.0 port is used, the Renesas´s xHCI host
controller μPD720202 is recommended.
• DELOCK 89348 (U3-PCIE1XG202-10)
PCIe 1x (μPD720202 host controller),
• DELOCK 89325 (U3X4-PCIE4XE101)
PCIe 4x (μPD720202 host controller)
USB 3.0
extension card
PCIe Slot
A PCIe 4x, 8x or 16x slot is recommended.
USB 3.0 Cable
USB 3.0 cable included in the packaging of your pco USB
3.0 camera
USB 3.0 Hub
Not recommended
Operating System
Windows XP 32 Bit, Win7 32 Bit / 64 Bit, Win8 32 Bit / 64 Bit
Multiple pco USB
3.0 cameras
connected to a PC
A separate DELOCK 89348 (U3-PCIE1XG202-10) extension
card for each camera should be used to guaranty maximum
data throughput of each camera.
FOL adapter
See Fehler! Verweisquelle konnte nicht gefunden werden.
Recommended Software Components
The pco USB 3.0 driver supports Windows 7/8 (x86 or x64) and
Windows XP x86 operating systems. Microsoft does not support USB
3.0 natively for Win 7 or older Windows versions. Therefore use the
manufacturers’ supplied xHCI host controller driver. By contrast, in
Windows 8 Microsoft does provide a native generic xHCI Driver but
pco recommends using the driver from the corresponding
manufacturer of the xHCI host controller. Currently there are seven
different vendors producing USB 3.0 host controllers:
•
•
•
Renesas Electronics
America Inc. (earlier NEC)
Texas Instrument Inc.
Intel Inc.
•
•
•
•
VIA Labs Inc (Diamond),
Fresco Logic Inc.
AMD Inc.
Etron Technology, Inc.
Please make sure that the most recent driver from the manufacturer
is installed before a pco USB 3.0 camera is connected to a USB 3.0
port of your computer. The appropriate driver for the USB 3.0 port
could be loaded from the website of the manufacturer of the USB 3.0
host xHCI controller; or from the website of the USB 3.0 extension
card vendor. The current driver of the extension card included in the
packaging of your pco USB 3.0 camera is added to the pco.USB
stick delivered with each pco.flim.
.
NOTE
To determine the driver currently in use for the xHCI controller, use
the Windows Device Manager. For example in Win 7, drop down
the USB-Controller entry and find the USB 3.0 host xHCI controller
of the USB 3.0 device in the sub-tree. Then right-click to open the
Properties dialog, click the driver tab and choose driver details
Before the pco.flim USB 3.0 camera is connected to the computer
the pco USB 3.0 Driver has to be installed. (see 4.1)
Please visit the support section of our website to get the latest USB
3.0 camera driver.
57
A2.2 INSTALLATION OF THE USB 3.0 CARD
An external USB 3.0 Host Controller Card is enclosed to each
pco.edge USB 3.0 camera.
Hardware Installation
First shut down your computer and install the USB 3.0 Host
Controller. Hardware Installation must be performed by a technician,
because high voltages can occur on the device.
WARNING
ELECTRIC SHOCK WARNING DUE TO VOLTAGE PARTS INSIDE
Risk of injury due to electrical shock.
 Always pull the main plug before opening the computer.
Driver Installation Instructions
• Within the provided installation files USB_HBA, open the folder
U3X4-PCIE4XE101, U3X4-PCIE1XE101, U3-PCIE1XG202.
• Open the subfolder Driver and run RENESAS-USB3-Host-Driver30230-setup.exe.
• If your current OS is Win7/8 and the User Account Control is
enabled, a dialog could occur asking, if you wish to launch the
setup: Accept with Yes.
•1 At first the installation is prepared.
•2 Secondly the software components are copied automatically.
•3 Finally the installation is completed and the Delock USB 3.0
extension card can be used.
1
2
3
58
A3 WATER COOLING PCO.AQUAMATIC II
A3 WATER COOLING PCO.AQUAMATIC II
A3.1 SYSTEM COMPONENTS
This is the re-cooling
system for pco cameras
with water-cooling.
1
2
3
5
4
Material No.
Description
1
30108000211
pco.aquamatic II
2
30108000212
Connection Tube 5m PVC 3541-01 PCO
(with Colder NS212 fittings)
3
50402000055
Power supply 1.2m
4
20307500024
Power cable
5
10307000130
WAT camera cable 5m FGG-RG58- NC3MX
6
10305000190
Innovatec Protect IP 1L
The operation of the pco.aquamatic is simple and uncomplicated.
Normally no maintenance and nearly no attention are needed. Only
the liquid level of the reservoir (water tank) should be controlled from
time to time.
6
Only use Innovatec Protect IP for the pco.aquamatic! Do not use
or add any other cooling liquid or normal water! If you need to add
cooling liquid in order to maintain level in the tank, please contact
PCO for additional supply.
The cooling liquid will turn yellow after some hours of operation. This
is normal and no sign of wear or malfunction. The optimum pH-value
is between 8 and 9 (please check this value if you are concerned
about the cooling liquid quality).
NOTE
The recommended service interval for the change of the cooling
liquid is four years.
59
A3.2 FIRST TIME INSTALLATION
5
3
4
2
Please take care to situate the unit on a flat and firm
surface. Do not cover the air intakes of the unit. Please
ensure free airflow around the pco.aquamatic to ensure
maximum cooling performance. All tubes and power
cords need to run kink-free.
Before installation of the unit carefully read the
Innovatek Protect IP safety datasheet (see Innovatek
Website).
outlet flow
inlet flow
Please follow steps 1 – 6.
1.
1 Connect tubes to cooling unit and camera. The two arrows on the
housing of the cooling unit only indicate flow direction. Either
connection of the camera can be used for in or out.
2.
2 Attach power connection.
3 Open tank cover.
3.
4 Turn power switch to on position (I).
4.
•5 Slowly fill in the cooling application mixture while the unit is
running, you have to constantly refill liquid.
•6 While the cooling liquid flows back to the reservoir make sure that
the whole air escapes from the system – this takes a few minutes
(move hoses if necessary).
The cooling liquid reservoir (tank) is filled when liquid
level is approximately 1-2 cm from the top of the tank.
The integrated pump only works when the pump
chamber is completely filled. To ensure this please
move hoses or remove air by evacuating. Reservoir
capacity is approximately 500 ml.
After steps 1 – 6 are completed successfully the
system is ready for operation.
NOTE
The hose connectors are waterproof in not connected state.
Maybe they lose one drop of cooling liquid from time to time. You
don’t have to empty the hoses while storing the camera system.
60
A3 WATER COOLING PCO.AQUAMATIC II
A3.3 OPERATION
First connect the power out of the cooling unit with the power
in of the pco.flim camera by using the PCO WAT camera
cable. Or you can use separate power supplies for camera and
aquamatic
The cooling unit provides two operation
modes.
Operation Mode on: the cooling unit is
turned on permanently and provides the
camera with power. Your camera can be
switchted on and off as needed.
Operation mode follow: the cooling unit will turn on when the
camera is switched on and vice versa.
Error Codes
The coolant temperature sensor is located in the water tank.
Temperature
27°C
36°C
55°C
60°C
Action
fan turns off
fan turns on
warning message
error message
If a warning level is passed, the Power LED blinks slowly and the
Error LED displays the error code. If a failure level is passed, the
Power LED blinks fast and the Error LED shows the error code.
Error / Failure
on
1Hz
flash
2Hz
flash
off
one short flash
1Hz
flash
two short
flashes
one short flash
none
Warning when temperature at 55 °C
(also if sensor is defect or missing)
Failure when temperature at 60 °C
Fan speed
(also if a high deviation of the standard value
is reached)
NOTE
The camera has its own protection circuit and will shut down
automatically when the electronics temperature exceeds safety
level.
61
A3.4 DIMENSIONS
All dimensions are given in millimeter.
Weight: 4kg (completely filled cooling liquid tank)
General Information
You are not restricted to purchase the pco.aquamatic system - it is
possible to use an own water cooling solution. A separate power
supply will be provided to every pco.flim camera with water
connectors. The hardware of the pco.flim cameras with USB 3.0
Interface is designed to work with or without a water cooling system,
because a fan that provides adequate cooling is inside the camera
anyway.
In case you use an own water cooling system, please make sure that
the liquid you use to cool your camera is at all times NEVER below
the dew point of the ambient temperature! In order to avoid any
appearance of condensation, use a cooling liquid at room
temperature. A liquid flow rate of 1–2 litres per minute is sufficient.
62
A4 IMAGE FILE FORMATS
A4 IMAGE FILE FORMATS
There are different file formats available for saving camera images
with Camware.
b16
The b16 16 bit format is similar to the bmp format. However, 16 bit
pixel values are used instead of 8 bit pixel values.
The file format consists either of a Basic Header (6 Long-parameter)
or of an Extended Header (32 Long-parameter), the latter of which is
optionally for additional information. There might follow a variable
comment field (ASCII code). Finally, there is the actual data set that is
saved linearly (as in the case of BMP files).
With the exception of the first value, all parameters are Long Integers
(4 Byte). The first 6 parameters must always exist. The rest of the
parameters, as well as the comment field, are optional.
1
2
3
4
5
6
7
8
9
10
Parameter
PCOfile size
header length
image width
image height
extended header
color mode
b/w min
b/w max
b/w linlog
Function
the first 4 byte are the characters PCOfile size in byte
header size + comment filed in byte
image width in pixel
image height in pixel
-1 (true), extended header follows
0 = black/with camera, 1 = color camera
black/white LUT-setting, minimum value
black/white LUT-setting, maximum value
black/white LUT-setting,
0 = linear, 1 = logarithmic
red LUT-setting, minimum value
red LUT-setting, maximum value
green LUT-setting, minimum value
green LUT-setting, maximum value
blue LUT-setting, minimum value
blue LUT-setting, maximum value
color LUT-setting, 0 = linear, 1 = logarithmic
11
red min
12
red max
13
green min
14
green max
15
blue min
16
blue max
17
color linlog
18

internal use
266
Comment file in ASCII characters with variable length of 0…XX.
The length of the comment filed must be documented in the header length field.
16 bit pixel data
line 1, pixel
1
line 1, pixel
2
…
value of the first pixel
value of the second pixel
…
We recommend that all images should be saved first in the b16 or
TIFF format. The advantage is to have the b16 or tiff images
available all the time. You will always have the maximum 16 bit
information. Please note that not all image analysis programs can
accommodate 16 bit data. The 8 bit format saves only the information
displayed on the monitor screen. The 16 bit information will be lost
and cannot be displayed later.
pcoraw
This 16 bit PCO file format is based on the new BigTIFF format, thus
allowing for file size > 4GB. A new PCO proprietary compression
scheme is added in case it is necessary
63
Standard File Formats
TIFF
Tag Image File Format, version 6.0 and lower. There is a 16bit
monochrome and color image format.
BMP
Windows Bitmap Format, b/w or color 8 bit format-images, which
have been saved in BMP format can be loaded later only as 8 bit
images, i.e. part of the original information (16 bit) is lost.
FTS
Flexible Image Transport System, Version 3.1. There is a 16 bit image
format. The NASA/Science Office of Standards and Technology
(NOST) has defined this format. Some programs use the FIT
extension for this format.
ASCII
16 bit format, some mathematical programs prefer ASCII data.
JPG
JPEG (named after the Joint Photographic Experts Group who
created the standard) is a commonly used method of lossy
compression for photographic images. The degree of compression
can be adjusted, allowing a selectable tradeoff between storage size
and image quality.
JP2
JPEG 2000 is a wavelet-based image compression standard and
coding system. It was created by the Joint Photographic Experts
Group committee in the year 2000 with the intention of superseding
their original discrete cosine transform-based JPEG standard
(created 1992).
AVI
Audio Video Interleave is a multimedia container format introduced by
Microsoft in November 1992 as part of its Video for Windows
technology.
MPG
MPEG-1, similar to JPEG, is a standard for lossy compression of
video and audio developed by the Moving Picture Experts Group
(MPEG).
WMV
Windows Media Video (WMV) is a compressed video compression
format for several proprietary codecs developed by Microsoft. The
original video format, known as WMV, was originally designed for
Internet streaming applications, as a competitor to RealVideo.
64
A5 CUSTOMER SERVICE
A5 CUSTOMER SERVICE
A5.1 SERVICE
The camera is designed to operate with no need of special
adjustments or periodic inspections.
A5.2 MAINTENANCE
CAUTION
NOTICE
NOTICE
NOTICE
NOTICE
UNPLUG CAMERA BEFORE CLEANING
Risk of injury due to electric shock!
 Unplug the camera from any power supply before cleaning it.
CLEANING
 Use a soft, dry cloth for cleaning the camera.
 Do not clean the input window unless it is absolutely
necessary.
 Be careful and avoid scratches and damage to the input
window surface.
 Do not use liquid cleaners or sprays.
LENS CLEANING
 The lens is best cleaned with pressurized air or with liquid
cleaners such as pure alcohol or with special optical
cleaners that are available at high quality photo stores.
 Use a cotton swab dipped in pure alcohol or optical cleaning
liquid and wipe only on the glass surface.
 Do not get any cleaning liquid on the metallic parts such as
the lens thread, because tiny detached particles may scratch
the surface.
CLEANING LIQUIDS
Aggressive cleaning liquids can damage your camera.
 Never use aggressive cleaning liquids such as gasoline,
acetone, spirits or nitro cleanser.
 Every time the input window is cleaned, there is the
possibility of surface damage.
PROTECTIVE CAP
Always store the camera with the protective cap or with a lens
mounted to avoid dust and dirt on the input window.
A5.3 RECYCLING
If you want to dispose your camera, please send it to PCO or take it
to a local recycling center.
The camera includes electronic devices, which can contain materials
harmful to the environment. These electronic devices must be
recycled.
65
A5.4 TROUBLE SHOOTING
If you have a question, which is not adequately addressed in this
manual, please contact PCO or your local dealer.
To speed your request, we need the following
information:
•
•
•
•
•
•
•
•
•
•
Short description of the problem
Description of your application
Camera settings
Type and version of camera software being used
Camera serial number
Operating system (PC)
Processor type (PC)
Memory
Graphic card
Graphic card setup
Please use the email options in the Help menu (see 6.4.7)
How to create a logfile:
•
•
•
•
•
Enable log files: File Menu Options MISC
(see 6.4.2.)
Reboot your pco.flim
Repeat the workflow which produces the faults.
Open the About Camware window
(Menu ? About) and click at the support links to
send an email directly to PCO-Support (see 6.4.7)
Or visit our website: http://www.pco.de/support
Repair
Before sending the camera for repair, first contact your local dealer
or PCO respectively.
When shipping the camera for repair, be certain to carefully pack the
camera with proper shipping materials. If possible use the original
packaging. Use the protection cap to protect the camera on the lens
thread.
Firmware, Software and Driver Update
You will find all necessary software and drivers on the accompanying
USB storage device.
For the latest versions please check pco website.
66
A6 INDEX
A6 INDEX
NOTE:
The mentioned page is
always the starting
page of a chapter!
Important keywords
Keyword
Camware software
Cooling
Data file load
Data sheet
Demo mode (Camware)
Dimensions
Driver installation
Help
Image Processing
Input / Output possibilities
Licensing
Logfile
[email protected] software
Maintenance
Modulation
Phase sequence
Preview image
Rear connections
Record sequence
Reference file load
Recycling
Reverse
Safety instructions
Save Results
Sequence configuration
Service
Support
Chapter
6
Page
12
A3
7.2.2
A1.2
6.4.1
A1.1
4
59
37
53
21
6.4.7
7.3.3
30
41
A1.3
7.5
6.9.7
7
A5.2
54
50
30
7.3.3
7.3.3
7.3.4
A1.3
7.3.4
7.2.1
A5.3
A1.3
2
7.2.3
7.3.3
A5.1
52
8
33
65
41
41
42
54
42
34
65
54
6
39
41
65
Time stamp
Timing
Trigger Mode
A5.4
6.3.4
6.3.1
6.3.1
66
19
15
15
USB 3.0
View Menu
Water cooling
A2
7.4
A3
56
45
59
67
ABOUT PCO
ABOUT PCO
pco.
In 1987, PCO was founded with the objective to develop and to
produce specialized fast and sensitive video camera systems, mainly
for scientific applications. Meanwhile the product range of PCO
cameras covers digital camera systems with high dynamic range,
high resolution, high speed and low noise, which are sold in the
scientific and industrial market all over the world.
Currently PCO is one of the leading manufacturers of scientific
cameras. Worldwide representatives, together with our own sales
department and technical support assure that PCO keeps in touch
with our customers and their needs. The actual wide range of
specialized camera systems is the result of technical challenge and
product specific know-how. A design according to advanced
techniques, a high standard of production and strict quality controls
guaranty a reliable operation of the cameras. Our own developments
in conjunction with an excellent contact to leading manufacturers of
image sensors ensure our access to state-of-the-art CCD- and
CMOS-technology for our cameras.
Since 2001, PCO is located in its own facility building in Kelheim at
the shore of the beautiful and international river Danube. Here in the
county Bavaria, which is well known for its excellent support and
conditions for high technology companies, we share the benefits of
the simple access to high performance products and services in the
surrounding area.
Kelheim itself is a historical town, first documented in 866. The small
city is founded at the confluence of the Danube and the Altmühl,
which has been converted into the Rhine-Main-Danube bypass
channel for water transport. Located in Danube-valley, it is the heart
of a beautiful river and forest covered lime plateau landscape. It´s
landmark, the Hall of Liberation, was built by Ludwig I. in 1863 on the
Mount Michael and is visible from all over the city and valley. The
beautiful Danube-Gorge, which is protected as natural monument
since 1840, is located between Kelheim and the famous abbey
Weltenburg.
68
pco.
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