Orbitrap Elite Hardware Manual
Part of Thermo Fisher Scientific
Thermo Fisher Scientific
Orbitrap Elite
Hardware Manual
Revision A - 1288170
© 2011 Thermo Fisher Scientific Inc. All rights reserved.
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Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo
Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use of this document, even if the information in the document is followed properly.
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Release History: Revision A released in June 2011.
For Research Use Only. Not for use in diagnostic procedures.
Place Declaration of Conformity here
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations. When the system is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety standards as described in the next section or sections by product name.
Changes that you make to your system may void compliance with one or more of these EMC and safety standards.
Changes to your system include replacing a part or adding components, options, or peripherals not specifically authorized and qualified by Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards, replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific or one of its authorized representatives.
Velos Pro Mass Spectrometer (April 2011)
EMC Directive 2004/108/EEC
EMC compliance has been evaluated by TUV Rheinland of North America, Inc.
EN 61326-1: 2006
EN 55011: 2007, A2: 2007
CFR 47, FCC Part 15, Subpart B, Class A: 2009
EN 61000-3-2: 2006
EN 61000-3-3: 1995, A1: 2001, A2: 2005
EN 61000-4-2: 1995, A1: 1999, A2: 2001
EN 61000-4-3: 2006
EN 61000-4-4: 2004
EN 61000-4-5: 2005
EN 61000-4-6: 2007
EN 61000-4-11: 2004
Low Voltage Safety Compliance
This device complies with Low Voltage Directive 2006/95/EEC and harmonized standard EN 61010-1:2001.
Velos Pro/ETD System (April 2011)
EMC Directive 2004/108/EEC
EMC compliance has been evaluated by TUV Rheinland of North America, Inc.
EN 61326-1: 2006
EN 55011: 2007, A2: 2007
CFR 47, FCC Part 15, Subpart B, Class A: 2009
EN 61000-3-2: 2006
EN 61000-3-3: 1995, A1: 2001, A2: 2005
EN 61000-4-2: 1995, A1: 1999, A2: 2001
EN 61000-4-3: 2006
EN 61000-4-4: 2004
EN 61000-4-5: 2005
EN 61000-4-6: 2007
EN 61000-4-11: 2004
Low Voltage Safety Compliance
This device complies with Low Voltage Directive 2006/95/EEC and harmonized standard EN 61010-1:2001.
FCC Compliance Statement
THIS DEVICE COMPLIES WITH PART 18 OF THE FCC RULES.
WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:
Thermo Fisher Scientific has contracted with one or more recycling/disposal companies in each EU
Member State, and this product should be disposed of or recycled through them. Further information on Thermo Fisher Scientific’s compliance with these Directives, the recyclers in your country, and information on Thermo Fisher Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www.thermo.com/WEEERoHS.
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Read This First
About This Guide
Who Uses This Guide
This Orbitrap Elite Hardware Manual contains a description of the modes of operation and principle hardware components of your
Orbitrap Elite instrument. In addition, this manual provides step-by-step instructions for cleaning and maintaining your instrument.
This Orbitrap Elite Hardware Manual is intended for all personnel that need a thorough understanding of the instrument (to perform maintenance or troubleshooting, for example). This manual should be kept near the instrument to be available for quick reference.
Scope of This Guide
Welcome to the Thermo Scientific Orbitrap Elite system! The Orbitrap
Elite is a member of the family of LTQ™ mass spectrometer (MS) hybrid instruments.
All information in this guide concerning the Orbitrap Elite mass spectrometer also applies to the Orbitrap Elite ETD system where the
ETD Module is physically coupled to the back of the Orbitrap Elite mass spectrometer.
Thermo Fisher Scientific
This manual includes the following chapters:
•
Chapter 1: “Functional Description” describes the principal
components of the Orbitrap Elite mass spectrometer.
•
Chapter 2: “Basic System Operations”
provides procedures for shutting down and starting up the Orbitrap Elite mass spectrometer.
•
Chapter 3: “User Maintenance” outlines the maintenance
procedures that you should perform on a regular basis to maintain optimum instrument performance.
•
Chapter 4: “Replaceable Parts”
lists the replaceable parts for mass spectrometer and data system.
•
Appendix A: “Fluoranthene” describes properties of the reagent that
is used in the ETD Module portion of the Orbitrap Elite ETD mass spectrometer.
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Related Documentation
Related Documentation
In addition to this guide, Thermo Fisher Scientific provides the following documents for Orbitrap Elite mass spectrometer and Orbitrap
Elite ETD mass spectrometer:
•
LTQ Orbitrap Series Preinstallation Requirements Guide
•
Orbitrap Elite Getting Started Guide
•
Velos Pro manual set
You can access PDF files of the documents listed above and of this guide from the data system computer. The software also provides Help.
❖
To view product manuals
1. From the Microsoft™ Windows™ taskbar, choose Start > Programs
> Thermo Instruments > LTQ > Manuals > model.
2. Click the PDF file that you want to view.
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Contacting Us
Contacting Us
There are several ways to contact Thermo Fisher Scientific.
Assistance
Customer Information Service
cis.thermo-bremen.com
is the Customer Information Service site aimed at providing instant access to
•
Latest software updates
•
Manuals, application reports, and brochures
Thermo Fisher Scientific recommends that you register with the site as early as possible.
To register, visit register.thermo-bremen.com/form/cis and fill in the registration form. Once your registration has been finalized, you will receive confirmation by e-mail.
Changes to the Manual
For technical support and ordering information, visit us on the Web: www.thermoscientific.com/ms
Service contact details for customers in Europe are available under: www.thermoscientific.com/euservicecontact
❖
To suggest changes to this manual
•
Please send your comments (in German or English) to:
Editors, Technical Documentation
Thermo Fisher Scientific (Bremen) GmbH
Hanna-Kunath-Str. 11
28199 Bremen
Germany
•
Send an e-mail message to the Technical Editor at [email protected]
You are encouraged to report errors or omissions in the text or index.
Thank you.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
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Typographical Conventions
Typographical Conventions
This section describes typographical conventions that have been established for Thermo Fisher Scientific manuals.
Data Input
Throughout this manual, the following conventions indicate data input and output via the computer:
•
Messages displayed on the screen are represented by capitalizing the initial letter of each word and by italicizing each word.
•
Input that you enter by keyboard is identified by quotation marks: single quotes for single characters, double quotes for strings.
•
For brevity, expressions such as “choose File > Directories” are used rather than “pull down the File menu and choose Directories.”
•
Any command enclosed in angle brackets < > represents a single keystroke. For example, “press <F1>” means press the key labeled
F1.
•
Any command that requires pressing two or more keys simultaneously is shown with a plus sign connecting the keys. For example, “press <Shift> + <F1>” means press and hold the <Shift> key and then press the <F1> key.
•
Any button that you click on the screen is represented in bold face letters. For example, “click Close”.
Topic Headings
The following headings are used to show the organization of topics within a chapter:
Chapter 1
Chapter Name
Second Level Topics
Third Level Topics
Fourth Level Topics iv
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Safety and EMC Information
Safety and EMC Information
In accordance with our commitment to customer service and safety, this instrument has satisfied the requirements for the European CE Mark including the Low Voltage Directive.
Designed, manufactured and tested in an ISO9001 registered facility, this instrument has been shipped to you from our manufacturing facility in a safe condition.
This instrument must be used as described in this manual. Any use of this instrument in a manner other than described here may result in instrument damage and/or operator injury.
Notice on Lifting and Handling of Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Scientific instrument requires a team
effort for lifting and/or moving the instrument. This instrument is too heavy and/or bulky for one person alone to handle safely.
Notice on the Proper Use of Thermo Scientific Instruments
In compliance with international regulations: If this instrument is used in a manner not specified by Thermo Fisher Scientific, the protection provided by the instrument could be impaired.
Notice on the Susceptibility to Electromagnetic Transmissions
Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency transmitters, such as mobile phones, in close proximity to the instrument.
Safety and Special Notices
Make sure you follow the precautionary statements presented in this guide. The safety and other special notices appear different from the main flow of text. Safety and special notices include the following:
Warning
Warnings highlight hazards to human beings. Each Warning is accompanied by a Warning symbol.
▲
Thermo Fisher Scientific
Caution
Cautions highlight information necessary to protect your instrument from damage.
▲
Note Notes highlight information that can affect the quality of your data. In addition, notes often contain information that you might need if you are having trouble.
▲
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Safety and EMC Information
Identifying Safety Information
This guide contains precautionary statements that can prevent personal injury, instrument damage, and loss of data if properly followed.
Warning symbols alert the user to check for hazardous conditions. These appear throughout the manual, where applicable. The most common warning symbols that appear in Thermo Fisher Scientific manuals are shown below.
In addition, every instrument has specific hazards. So, be sure to read and comply with all precautions described in this guide. They will help to ensure the safe and long-term use of your system.
Warning
General Hazard. This general symbol indicates that a hazard is present that could result in injuries if it is not avoided.
The source of danger is described in the accompanying text.
▲
Warning
Electric Shock Hazard. High voltages capable of causing personal injury are used in the instrument. The instrument must be shut down and disconnected from line power before service is performed. Do not operate the instrument with the top cover off. Do not remove protective covers from PCBs.
▲
Warning
Burn Hazard. Treat heated zones with respect. Parts of the instrument might be very hot and might cause severe burns if touched.
Allow hot components to cool before servicing them.
▲
Warning
Corrosive Material. Wear gloves when handling toxic, carcinogenic, mutagenic, or corrosive/irritant chemicals. Use approved containers and procedures for disposal of waste solution.
▲
General Safety Precautions
Observe the following safety precautions when you operate or perform service on your instrument:
•
The system should be operated by trained personnel only. Read the manuals before starting the system and make sure that you are familiar to the warnings and safety precautions!
•
Accurate results can be obtained only, if the system is in good condition and properly calibrated.
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Thermo Fisher Scientific
Read This First
Safety and EMC Information
•
Service by the customer should be performed by trained qualified personnel only and should be restricted to servicing mechanical parts! Service on electronic parts should be performed by Thermo
Fisher Scientific field service engineers only!
•
Before plugging in any of the instrument modules or turning on the power, always make sure that the voltage and fuses are set appropriately for your local line voltage.
•
Only use fuses of the type and current rating specified. Do not use repaired fuses and do not short-circuit the fuse holder.
•
The supplied power cord must be inserted into a power outlet with a protective earth contact (ground). When using an extension cord, make sure that the cord also has an earth contact.
•
Do not change the external or internal grounding connections.
Tampering with or disconnecting these connections could endanger you and/or damage the system.
•
The instrument is properly grounded in accordance with regulations when shipped. You do not need to make any changes to the electrical connections or to the instrument’s chassis to ensure safe operation.
•
Never run the system without the housing on. Permanent damage can occur. When leaving the system, make sure that all protective covers and doors are properly connected and closed, and that heated areas are separated and marked to protect for unqualified personnel!
•
Do not turn the instrument on if you suspect that it has incurred any kind of electrical damage. Instead, disconnect the power cord and contact a Thermo Fisher Scientific field service engineer for a product evaluation. Do not attempt to use the instrument until it has been evaluated. (Electrical damage may have occurred if the system shows visible signs of damage, or has been transported under severe stress.)
•
Damage can also result if the instrument is stored for prolonged periods under unfavorable conditions (for example, subjected to heat, water, etc.).
•
Always disconnect the power cord before attempting any type of maintenance.
•
Capacitors inside the instrument may still be charged even if the instrument is turned off.
•
Never try to repair or replace any component of the system that is not described in this manual without the assistance of your Thermo
Fisher Scientific field service engineer.
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Safety and EMC Information
•
Do not place any objects upon the instrument—especially not containers with liquids—unless it is requested by the user documentation. Leaking liquids might get into contact with electronic components and cause a short circuit.
Safety Advice for Possible Contamination
Hazardous Material Might Contaminate Certain Parts of Your
System During Analysis.
In order to protect our employees, we ask you to adhere to special precautions when returning parts for exchange or repair.
If hazardous materials have contaminated mass spectrometer parts,
Thermo Fisher Scientific can only accept these parts for repair if they have been properly decontaminated. Materials that due to their structure and the applied concentration might be toxic or that are reported in publications to be toxic are regarded as hazardous. Materials that will generate synergetic hazardous effects in combination with other present materials are also considered hazardous.
Your signature on the Health and Safety Form confirms that the returned parts have been decontaminated and are free of hazardous materials. Download the form from decon.thermo-bremen.com
or order it from the Thermo Fisher Scientific field service engineer.
Parts contaminated by radioisotopes should not be returned to Thermo
Fisher Scientific—neither under warranty nor within the exchange part program. If unsure about parts of the system possibly being contaminated by hazardous material, please make sure the Thermo
Fisher Scientific field service engineer is informed before the engineer starts working on the system.
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Contents
Thermo Fisher Scientific
Chapter 1 Functional Description.............................................................1-1
General Description .......................................................... 1-2
Mechanical Characteristics ............................................. 1-3
Specifications ................................................................. 1-4
Control Elements.............................................................. 1-5
System Status LEDs ....................................................... 1-5
Control Panels ............................................................... 1-6
Linear Ion Trap............................................................... 1-12
Curved Linear Trap ........................................................ 1-13
Orbitrap Analyzer ........................................................... 1-14
Extraction of Ion Packets ............................................. 1-14
Measuring Principle ..................................................... 1-15
Ion Detection............................................................... 1-16
Active Temperature Control ........................................ 1-17
HCD Cell ....................................................................... 1-18
HCD and ETD ........................................................... 1-18
ETD System ................................................................... 1-19
Principle of Operation ................................................. 1-21
ETD Module ............................................................... 1-21
Vacuum System .............................................................. 1-30
Turbomolecular Pumps................................................ 1-31
Forepumps of the Linear Trap ..................................... 1-33
Forepump of the ETD Module.................................... 1-34
Vacuum System Controls............................................. 1-35
Vacuum System Heating during a System Bakeout ...... 1-36
Gas Supply...................................................................... 1-37
Gas Supply for the Mass Analyzers ............................... 1-37
Gas Supply of the Reagent Ion Source ......................... 1-40
Cooling Water Circuit .................................................... 1-42
Recirculating Chiller .................................................... 1-43
Properties of Cooling Water......................................... 1-43
Printed Circuit Boards .................................................... 1-44
Linear Ion Trap Electronics.......................................... 1-45
Electronic Boards on the Right Side of the
Instrument ................................................................... 1-46
Electronic Boards on the Left Side of the Instrument ... 1-59
Chapter 2 Basic System Operations ........................................................2-1
Shutting Down the System in an Emergency .................... 2-2
Behavior of the System in Case of a Main Failure........... 2-2
Placing the Instrument in Standby Condition................... 2-4
Placing the ETD Module in Standby Condition ............ 2-4
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Contents
Placing the MS in Standby Condition............................ 2-6
Shutting Down the Orbitrap Elite Mass Spectrometer
Completely ....................................................................... 2-7
Shutting Down the Instrument ...................................... 2-7
Starting Up the System after a Shutdown.......................... 2-9
Starting Up the Instrument ............................................ 2-9
Setting Up Conditions for Operation........................... 2-10
Starting the ETD Module After a Complete
Shutdown .................................................................... 2-11
Resetting the System ....................................................... 2-12
Resetting Tune and Calibration Parameters to their
Default Values ................................................................ 2-13
Turning Off the Reagent Ion Source: What to Expect .... 2-14
Chapter 3 User Maintenance.................................................................... 3-1
General Remarks............................................................... 3-2
Returning Parts .............................................................. 3-3
Cleaning the Surface of the Instrument .......................... 3-3
Maintenance of the Vacuum System ................................. 3-4
Baking Out the System .................................................. 3-4
Maintenance of the Forepumps...................................... 3-5
Maintenance of the Turbomolecular Pumps ................ 3-11
Maintenance of the ETD Module ................................... 3-12
Handling and Cleaning Reagent Ion Source Parts........ 3-13
Removing the Access Panels ......................................... 3-18
Maintenance of the Reagent Ion Source ....................... 3-20
Replacing Inlet Valve Components .............................. 3-45
Replacing the Reagent Vials ......................................... 3-48
Cleaning the Fan Filters of the ETD Module............... 3-57
Maintenance of the Cooling Circuit................................ 3-58
Maintenance for the Recirculating Chiller.................... 3-58
Replacing the Water Filter Cartridge............................ 3-58
Chapter 4 Replaceable Parts .................................................................... 4-1
Ion Sources ....................................................................... 4-2
Parts for the Basic System.................................................. 4-3
Parts Lists for the ETD System ......................................... 4-5
ETD Reagent Kit ........................................................... 4-7
Glossary ................................................................................... G-1
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Figures
Thermo Fisher Scientific
Orbitrap Elite MS front view ................................................................ 1-2
Schematic of the Orbitrap Elite MS ...................................................... 1-3
Top lid of MS portion opened .............................................................. 1-4
Right side of the Orbitrap Elite MS ...................................................... 1-6
Power control panel with power control LEDs and switches ................. 1-8
External connections to the Orbitrap Elite MS ................................... 1-10
Schematic of Orbitrap cell and example of stable ion trajectory ........... 1-14
Approximate shape of ion packets of different m/z after
stabilization of voltages ........................................................................ 1-16
Orbitrap Elite ETD MS front view ..................................................... 1-19
Schematic of the Orbitrap Elite ETD MS ........................................... 1-20
Orbitrap Elite ETD MS, rear side ....................................................... 1-22
Rear view of the ETD Module, with major component locations ........ 1-22
ETD Module functional block diagram .............................................. 1-23
Right side of the Orbitrap Elite ETD MS ........................................... 1-24
ETD Power Module panel .................................................................. 1-24
Reagent Ion Source dialog box ............................................................ 1-27
Reagent ion source schematics ............................................................. 1-29
Vacuum components on the left instrument side ................................ 1-31
Vacuum components on the right instrument side .............................. 1-32
Forepump for ETD Module ............................................................... 1-34
Schematic of gas supply for Orbitrap Elite ETD MS ........................... 1-37
Proper orientation of the Swagelok-type nut and two-piece ferrule ...... 1-38
ETD reagent carrier gas port at the ETD Module ............................... 1-40
Schematic of cooling water circuit ....................................................... 1-42
Electronic connections to linear trap ................................................... 1-45
Electronic boards on the right side of the Orbitrap Elite MS ............... 1-46
ETD Ion Optic Supply board ............................................................. 1-47
Data Acquisition Digital PCI board .................................................... 1-50
Data Acquisition Analog board ........................................................... 1-51
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Figures
Instrument Control board ................................................................... 1-52
Power Distribution board ................................................................... 1-54
Power Supply 1 board ......................................................................... 1-57
Electronic boards on the left side of the instrument ............................. 1-59
Ion Optic Supply board ...................................................................... 1-60
Central Electrode Pulser board ............................................................ 1-61
Temperature Controller board ............................................................ 1-62
CLT RF unit (cover removed) ............................................................. 1-64
Central Electrode Power Supply board ................................................ 1-65
High Voltage Power Supply board (cover removed) ............................ 1-67
Main power switch in Off position ....................................................... 2-2
Tune Plus window (Orbitrap Elite ETD), toolbar ................................. 2-4
Placing the reagent ion source in Standby condition ........................... 2-14
Schematic of ETD forepump ................................................................ 3-6
Accessing the ETD Forepump: Removing the panel ............................. 3-7
Hooks (left) and top side of detached bottom panel (right) ................... 3-7
Lugs for fixing the bottom panel ........................................................... 3-8
Gas ballast control positions .................................................................. 3-9
Routine maintenance sequence for ETD system .................................. 3-12
Rear view of the ETD Module ............................................................ 3-18
Ion source components (left view) ....................................................... 3-21
Tune Plus window (Orbitrap Elite ETD) ............................................ 3-22
Ion volume tool components ............................................................... 3-23
Guide bar being inserted into guide bar opening ................................. 3-23
Guide bar insertion complete .............................................................. 3-24
Rear view of the ETD Module, showing the inlet valve ....................... 3-24
Ion volume tool handle in the unlock position .................................... 3-25
Ion volume tool guide bar first stop ..................................................... 3-25
Reagent Ion Source dialog box, Open Probe Interlock button. ............ 3-26
Instrument Message box: The Ball Valve can now be opened .............. 3-26
Ion volume tool inserted into the inlet valve ........................................ 3-27
Detail of ion volume tool fully inserted into the inlet valve ................. 3-27
Ion volume tool handle in the locked position .................................... 3-28
Ion volume assembly ........................................................................... 3-29
Separating ion volume and ion volume holder .................................... 3-29
Placing the ion volume on the ion volume tool ................................... 3-30
Ion volume tool handle in the unlock position .................................... 3-31
Ion volume tool handle in the locked position .................................... 3-32
Inlet valve components (ion volume tool not shown) .......................... 3-34
Valve shield (1) covering the vacuum manifold probe plate ................. 3-35
Removing the foreline hose from its connection .................................. 3-35
Unscrewing the vacuum manifold probe plate ..................................... 3-36
Removing the vacuum manifold probe plate ....................................... 3-36
Interior of vacuum manifold ............................................................... 3-37
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Figures
Removing the ion source assembly from the vacuum manifold ........... 3-38
Ion source assembly exploded view ...................................................... 3-39
Ion source, exploded view ................................................................... 3-41
Ion source lens assembly and ion source .............................................. 3-43
Inlet valve components ........................................................................ 3-45
Inlet valve seal tool inserted in the inlet valve ...................................... 3-46
Inlet valve seal on the inlet valve seal tool ............................................ 3-47
Inlet valve seal disengaged from tool .................................................... 3-47
Reagent Ion Source dialog box ............................................................ 3-50
ETD Module with back panel removed .............................................. 3-52
Reagent vials with holders ................................................................... 3-53
ETD Module with vial heater cover removed ...................................... 3-53
Reagent inlet assembly ........................................................................ 3-56
ETD Module, top panel ...................................................................... 3-57
Removing the filter cartridge ............................................................... 3-60
Filter cartridge with Quick couplers .................................................... 3-60
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Tables
System status LEDs of the Orbitrap Elite MS ....................................... 1-5
Circuit breakers of the Orbitrap Elite MS ............................................. 1-7
Typical pressure readings in the ETD Module .................................... 1-35
Diagnostic LEDs on the ETD Ion Optic Supply board ....................... 1-47
Diagnostic LEDs on the Preamplifier board ........................................ 1-49
Diagnostic LEDs of the Data Acquisition Digital PCI board .............. 1-50
Diagnostic LEDs of the Data Acquisition Analog board ...................... 1-51
Diagnostic LEDs of the Power Supply 2 board ................................... 1-52
Diagnostic LEDs of the Instrument Control board ............................. 1-53
Software status LEDs of the Instrument Control board ....................... 1-53
Status LEDs of the Power Distribution board ..................................... 1-55
Working modes of the Power Distribution board ................................ 1-56
Operating states of the Power Distribution board ............................... 1-56
Diagnostic LEDs of the Power Supply 1 board ................................... 1-58
Diagnostic LEDs of the Ion Optic Supply board ................................. 1-60
Diagnostic LEDs of the Central Electrode Pulser board ...................... 1-62
Diagnostic LEDs of the Temperature Controller board ....................... 1-63
Diagnostic LEDs of the CLT RF Main board ..................................... 1-64
Diagnostic LEDs of the Central Electrode Power Supply board .......... 1-66
Diagnostic LEDs of the High Voltage Power Supply board ................ 1-68
User maintenance procedures ................................................................ 3-2
Indications requiring maintenance of the ETD system ........................ 3-13
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Procedures
To view product manuals...........................................................................ii
To suggest changes to this manual.............................................................iii
To place the ETD Module in Standby condition ................................... 2-4
To place the Orbitrap Elite system in Standby condition ....................... 2-6
To shut down the instrument completely............................................... 2-7
To shut down the Orbitrap Elite system ................................................ 2-7
To start up the Orbitrap Elite mass spectrometer ................................... 2-9
To set up your Orbitrap Elite mass spectrometer for operation ............ 2-10
To start up the ETD Module after a complete shutdown ..................... 2-11
To reset the Orbitrap Elite MS tune and calibration parameters........... 2-13
To perform a system bakeout ................................................................. 3-4
To add oil to the ETD forepump........................................................... 3-8
To purge the rotary-vane pump oil......................................................... 3-9
To change the ETD forepump oil ........................................................ 3-10
To clean reagent ion source stainless steel parts .................................... 3-15
To clean the non-stainless-steel portions of hybrid parts....................... 3-17
To remove the ETD main access panel ................................................ 3-18
To remove the ETD side access panel .................................................. 3-19
To clean the ion volume with an inlet valve ......................................... 3-22
To reinsert the ion volume ................................................................... 3-29
To clean the ion source lens assembly................................................... 3-33
To clean the ion source block............................................................... 3-40
To replace the ion source filament........................................................ 3-43
To replace inlet valve components........................................................ 3-45
To place the Orbitrap Elite ETD mass spectrometer in Off
To install or exchange the reagent vials................................................. 3-51
To change the reagent ion source flow restrictors ................................. 3-54
To clean the fan filters of the ETD Module ......................................... 3-57
To replace the water filter cartridge ...................................................... 3-59
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
xvii
Chapter 1
Functional Description
This chapter provides an overview of the functional elements of the
Orbitrap Elite mass spectrometer. It contains the following topics:
•
“General Description” on page 1-2
•
“Control Elements” on page 1-5
•
“Linear Ion Trap” on page 1-12
•
“Curved Linear Trap” on page 1-13
•
“Orbitrap Analyzer” on page 1-14
•
•
•
•
“Cooling Water Circuit” on page 1-42
•
“Printed Circuit Boards” on page 1-44
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-1
Functional Description
General Description
General Description
The Orbitrap Elite mass spectrometer is a hybrid mass spectrometer incorporating the Velos Pro™ dual cell linear trap and the high-field
Orbitrap™ analyzer.
shows a front view of the instrument.
System status LEDs of linear trap System status LEDs of Orbitrap Elite MS
Orbitrap Analyzer
Linear Trap
Forepumps cabinet
Figure 1-1. Orbitrap Elite MS front view
The Orbitrap Elite mass spectrometer consists of four main components
.), which are described in the following topics:
•
Dual cell linear ion trap (Thermo Scientific Velos Pro) for sample ionization, precursor ion selection, fragmentation, and AGC™.
•
Intermediate storage device (curved linear trap) that is required for short pulse injection.
•
High-field Orbitrap analyzer for Fourier transformation based analysis.
•
Collision cell for performing higher energy CID experiments.
The Orbitrap Elite ETD mass spectrometer has an additional reagent ion source for performing Electron Transfer Dissociation (ETD)
experiments. See “ETD System” on page 1-19
.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Electrospray Ion Source S-Lens
Velos Pro MS
Square Quadrupole with neutral blocking
Octopole High Pressure Cell Low Pressure Cell
Functional Description
General Description
Multipole
Orbitrap analyzer
C-Trap HCD Cell
High-field Orbitrap Mass Analyzer
Figure 1-2. Schematic of the Orbitrap Elite MS
Mechanical Characteristics
Wheels at the bottom side of the instrument facilitate positioning the
Orbitrap Elite mass spectrometer at the intended place in the laboratory.
The mains inlet as well as a power outlet for peripheral devices are located at the right side of the instrument. The forepumps for the vacuum system of the linear trap and the Orbitrap analyzer are hidden under the linear trap and accessible from the front. The forepump for the ETD Module is accessible after removing the bottom panel of the rear side. The left side panel and the front panel of the MS portion are mounted on hinges and the right side panel is removable. The top lid of the MS portion opens upwards to allow easy access for Thermo Fisher
Scientific field service engineers from the top. See Figure 1-3
.
In the Orbitrap Elite ETD mass spectrometer, after removing the cables the top lid of the ETD Module is also removable to allow accessing its electronic components.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-3
Functional Description
General Description
Specifications
Figure 1-3. Top lid of MS portion opened
A stand-alone recirculating water chiller is shipped with the instrument.
It is connected to the right side of the instrument.
The Orbitrap Elite mass spectrometer has the following measuring specifications:
Resolution
60000 (FWHM) at m/z 400 at a scan rate of 4 Hz, minimum resolution 15000 (FWHM) maximum resolution > 240000 (FWHM) at m/z 400
Cycle Time
> 4 scans at 60000 (FWHM) resolution @ m/z 400 per second
m/z 50–2000; m/z 200–4000
Mass Range
Mass Accuracy
<3 ppm RMS for 2 h period with external calibration using defined conditions,
<1 ppm RMS with internal calibration
Dynamic Range >10000 between mass spectra,
>5000 between highest and lowest detectable ion signal in one spectrum
MS/MS
MS/MS and MS n
scan functions
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Control Elements
System Status LEDs
Functional Description
Control Elements
The Orbitrap Elite mass spectrometer is mainly operated from the desktop computer (data system). Some control elements for important system functions are located directly on the instrument. They are described in the following sections.
Figure 1-4 shows the system status LEDs at the front of the instrument.
Five LEDs indicate the main functions of the system. (See also
Figure 1-5 on page 1-6 .) The Power LED is directly controlled by the
3 × 230 V input and all other LEDs are controlled by the power distribution board (See
“Power Distribution Board” on page 1-53 ).
explains the function of the various LEDs.
Thermo Fisher Scientific
Figure 1-4. System status LEDs
The system status LEDs at the front panel of the linear ion trap are described in the LTQ Series Hardware Manual.
Table 1-1. System status LEDs of the Orbitrap Elite MS
LED
Power
Status
Green
Off
Vacuum a
Green
Yellow
Communication Green
Information
Main switch on
Main switch off
Operating vacuum reached
Insufficient vacuum or Vacuum Pumps switch off
Yellow
Communication link between instrument and data system established
Communication link starting up or Vacuum Pumps switch off
System a
Green
Yellow
System ready
FT Electronics switch off or Vacuum Pumps switch off
Detect Blue
Off
Orbitrap analyzer is scanning
Orbitrap analyzer is not scanning a
These LEDs are flashing when a system bakeout is performed. See “Baking Out the System” on page 3-4
.
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-5
Functional Description
Control Elements
Control Panels
Power panel of linear trap
shows the right side of the Orbitrap Elite mass spectrometer.
Located here are the control panels, switches, and the ports for the external connections (mains supply, gases, Ethernet communication, and cooling water).
Bakeout timer
Forepumps cabinet
Cover lid for bakeout controls
Switches and control LEDs
Main power switch
Power connector
Figure 1-5. Right side of the Orbitrap Elite MS
For more information about the external connections, see “External
.
Upper Control Panel
The upper instrument control panel comprises the bakeout timer, the bakeout control buttons, and three circuit breakers. To access the upper control panel, swing open the small lid (opens from left to right). See
and
The timer allows setting the duration for the bakeout of the system.
After the duration is set, the bakeout procedure is started by pressing the green button on the right. A running bakeout procedure can be stopped by pressing the orange button on the left side. For instructions about performing a bakeout, see
“Baking Out the System” on page 3-4
.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Control Elements
Bakeout timer
Bakeout control buttons
Circuit breakers
Cover lid
Power Control Panel
Thermo Fisher Scientific
Figure 1-6. Upper control panel
Note The buttons themselves have no indicator function. A running bakeout procedure is indicated by flashing Vacuum and System LEDs at the front side of the instrument. See
▲
Three circuit breakers are located at the bottom of this control panel.
shows the parts of the Orbitrap Elite mass spectrometer that are protected by the respective circuit breaker. The proper function of each circuit breaker is signaled by a dedicated LED in the power control panel (for example, F1 corresponds to L1).
Table 1-2. Circuit breakers of the Orbitrap Elite MS
F2
F3
Circuit breaker Ampere LED Instrument parts
F1 10 L1 Power Distribution
15
15
L2
L3
Linear ion trap
Multiple socket outlets (Peripherals, LC, heater, etc.)
control LEDs above the Vacuum Pumps switch at the right side. See
Figure 1-7 . They indicate whether the corresponding circuit breaker is
closed and the respective parts of the instrument have power. (See
.)
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-7
Functional Description
Control Elements
Power control LEDs
Vacuum Pumps switch
FT Electronics switch
Figure 1-7. Power control panel with power control LEDs and switches
The use of the switches below the power control LEDs changes the working mode of the power distribution. (See “Working Modes of the
Power Distribution” on page 1-48 .)
The Vacuum Pumps switch can be set into the positions ON or OFF.
When the switch is in the OFF position, everything but the multiple socket outlet is switched off.
The FT Electronics switch can be set into the operating position (ON) or into the service position (OFF). When the switch is in the service position, all components are switched off with exception of the following:
•
Fans
•
Heater control
•
Power distribution (See
“Power Distribution Board” on page 1-53 )
•
Pumps (See “Vacuum System” on page 1-30 )
•
Temperature controller (See
“Temperature Controller Board” on page 1-62 )
•
Vacuum control
The linear ion trap also remains on because it has a separate Service switch.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Main Power Switch
Functional Description
Control Elements
The main power switch must be turned 90° clockwise/anti-clockwise to
switch on/off the instrument (see Figure 1-8
). Placing the main power switch in the Off position turns off all power to the Orbitrap Elite mass spectrometer, the linear ion trap, and the vacuum pumps. In the
Orbitrap Elite ETD mass spectrometer, power to the ETD Module is also turned off.
On
External Connections
Thermo Fisher Scientific
Off
Figure 1-8. Main power switch
Note When the main power switch is in the Off position, you can secure it with a padlock or a cable tie (to prevent unintended re-powering when performing maintenance, for example).
▲
Figure 1-9 on page 1-10 shows the lower right side of the instrument
with the external connections for mains supply, gases, cooling water, and
Ethernet communication.
Located at the top are two ports for Ethernet cables for connecting the
Orbitrap Elite mass spectrometer and the linear ion trap via an Ethernet hub with the data system computer.
The power outlet for peripheral devices is located below the Ethernet ports. In the Orbitrap Elite mass spectrometer, the outlet provides the mains supply for the data system. In the Orbitrap Elite ETD mass spectrometer, the outlet provides the mains supply for the ETD Module whereas the data system is connected to a wall outlet.
The power connector for the mains supply is located at the center. The
Orbitrap Elite mass spectrometer is designed to operate at a nominal voltage of 230 V AC, 50/60 Hz. Line voltages can vary between a minimum of 207 V AC and a maximum of 253 V AC.
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-9
Functional Description
Control Elements
1
Caution
Systems installed in areas with 208 V power experience voltage sags during high use periods that might place the line voltage below the operating parameters discussed in this section. In this case, you must protect your instrument by using a buck/boost transformer to ensure that power is within the specified parameters at all times.
▲
Ethernet ports
Power outlet for peripheral devices
Power connector
Helium gas inlet
Collision gas inlet
Nitrogen gas inlet
Cooling water inlet port
Cooling water outlet port
Figure 1-9. External connections to the Orbitrap Elite MS
The cooling water ports are located below the power connector. (See also
“Cooling Water Circuit” on page 1-42 .)
The port for nitrogen gas allows connecting a Teflon® hose from the gas supply of the laboratory to the instrument. The required gas pressure for nitrogen is 690 ± 140 kPa (6.9 ± 1.4 bar, 100 ± 20 psi). Helium
(40 ±10 psi [275 ±70 kPa], 99.999% [ultra-high] purity) enters the instrument through a 1/8 inch port. Metal tubing from the helium gas supply must be terminated with 1/8 inch, female, Swagelok-type
connectors. See “Gas Supply” on page 1-37
for information about connecting the gas supplies to the instrument.
Caution
Do not connect other gases than nitrogen or helium to the
Orbitrap Elite mass spectrometer! The maximum pressure for the nitrogen gas inlet is 830 kPa (8.3 bar, 120 psi); the maximum pressure for the helium inlet is 345 kPa (3.45 bar, 50 psi).
▲
1
The port named Collision Gas is not used in the Orbitrap Elite MS.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Control Elements
In the Orbitrap Elite ETD mass spectrometer, the ETD reagent carrier gas supply of the laboratory is connected via metal tubing to an1/8 inch inlet port at the rear side of the instrument. Metal tubing from the gas supply must be terminated with 1/8 inch, female, Swagelok-type connectors. The required gas pressure is 690 ± 140 kPa (6.9 ± 1.4 bar,
100 ± 20 psi). See
“Gas Supply of the Reagent Ion Source” on page 1-40
.
The exhaust hose from the rotary pumps comes out the back of the instrument, and connects the pumps to the exhaust system in the laboratory.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-11
Functional Description
Linear Ion Trap
Linear Ion Trap
The Orbitrap Elite system can utilize a variety of ionization techniques such as ESI, APCI, or APPI. Maintenance of the Ion Max API source, as well as switching between ionization methods, is vent-free. Ions are transferred by octapole and “square” quadrupole lenses into an ion trap that is optimized for axial ion ejection into the curved linear trap. (See
.)
The linear ion trap is an independent MS detector (Thermo Scientific
Velos Pro), which can store, isolate, and fragment ions and then send them either to the Orbitrap analyzer for further analysis or to an
SEM detector. The linear ion trap is a unique ion preparation and injection system for Orbitrap MS, because it has greater ion storage capacity than conventional 3D ion trap devices. The linear ion trap is completely described in the LTQ Series Hardware Manual.
All the ion handling, selection and excitation capabilities of the ion trap can be used to prepare ions for analysis in the Orbitrap analyzer. These features include storage and ejection of all ions, storage of selected
m/z ranges, as well as ion isolation. Isolated ions can be excited and then fragmented as necessary for MS/MS and MS n
experiments. The patented Automatic Gain Control (AGC) provides extended dynamic range and insures optimized overall performance of the ion trap and
Orbitrap MS.
The application of a supplementary RF voltage on the end lenses of the linear trap allows ions of opposite polarity to be trapped in the same space at the same time (charge-sign independent trapping—CSIT). This allows performing ion-ion reactions of previously isolated precursor cations with ETD reagent anions.
The linear ion trap and the transfer chamber are mounted on a table.
. The table also serves as a housing for the forepumps. See
Figure 1-26 on page 1-33 . The Orbitrap Elite mass
spectrometer provides power for the linear ion trap. The Orbitrap
Elite ETD mass spectrometer also provides the power for the
ETD Module.
The linear ion trap is delivered with power connector, gas lines (He,
N
2
), and vacuum tube lines extending to the ESI source. On the rear side of the Velos Pro ion trap is a flange with an O-ring seal. When the flange is removed, the Orbitrap transfer chamber is mounted to the flange of the linear ion trap. The transfer chamber is held with supports on the table. The components of the ion optics and the Orbitrap analyzer are fixed to the transfer chamber.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Curved Linear Trap
Functional Description
Curved Linear Trap
On their way from the linear trap to the Orbitrap analyzer, ions move through the gas-free RF-only octapole into the gas-filled curved linear trap (C-Trap). See
Figure 1-10 . Ions entering the C-Trap loose their
kinetic energy in collisions with nitrogen bath gas emanating from the
HCD cell and get collected near the middle part of the C-Trap. The nitrogen collision gas (bath gas) is used for dissipating the kinetic energy of injected ions to cool them down to the axis of the C-Trap.
Voltages on the end apertures of the curved trap (entrance and exit apertures) are elevated to provide a potential well along its axis. These voltages may be later ramped up to squeeze ions into a smaller package along this axis. The RF to the C-Trap (“Main RF”) is provided by the
CLT RF main board. (See page 1-63 .) Entrance and exit DC voltages as
well as RF voltages to the transfer multipole are all provided by the ion
optic supply board. (See page 1-59 .) High voltages to the lenses are
provided by the high voltage power supply board. (See
Multipole Entrance C-Trap Exit Collision Cell
Static
Pulsing from Velos Pro
Squeezing in C-Trap
Figure 1-10. Layout of the Orbitrap Elite MS, also showing the applied voltages
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-13
Functional Description
Orbitrap Analyzer
Orbitrap Analyzer
The heart of the Orbitrap™ analyzer is an axially-symmetrical mass analyzer. It consists of a spindle-shape central electrode surrounded by a
pair of bell-shaped outer electrodes. See Figure 1-11
. The Orbitrap analyzer employs electric fields to capture and confine ions.
r z
Figure 1-11. Schematic of Orbitrap cell and example of stable ion trajectory
Extraction of Ion Packets
For ion extraction, the RF on the rods of the C-Trap is ramped off and extracting voltage pulses are applied to the electrodes, pushing ions orthogonally to the curved axis through a slot in the inner electrode.
Because of the initial curvature of the C-Trap and the subsequent lenses, the ion beam converges on the entrance into the Orbitrap analyzer. The lenses that follow the C-Trap (Z-lens) form also differential pumping slots and cause spatial focusing of the ion beam into the entrance of the
Orbitrap analyzer. Ions are electrostatically deflected away from the gas jet, thereby eliminating gas carryover into the Orbitrap analyzer.
Owing to the fast pulsing of ions from the C-Trap, ions of each mass-to-charge ratio arrive at the entrance of the Orbitrap analyzer as short packets only a few millimeters long. For each mass-to-charge population, this corresponds to a spread of flight times of only a few hundred nanoseconds for mass-to-charge ratios of a few hundred
Daltons/charge. Such durations are considerably shorter than a half-period of axial ion oscillation in the trap. When ions are injected into the Orbitrap analyzer at a position offset from its equator (See
.), these packets start coherent axial oscillations without the need for any additional excitation cycle.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Orbitrap Analyzer
Measuring Principle
Figure 1-12. Principle of electrodynamic squeezing of ions in the Orbitrap analyzer as the field strength is increased
The evolution of an ion packet during the increase of the electric field is
shown schematically on Figure 1-12
. When the injected ions approach the opposite electrode for the first time, the increased electric field
(owing to the change of the voltage on the central electrode) contracts the radius of the ion cloud by a few percent. The applied voltages are adjusted to prevent collision of the ions with the electrode. A further increase of the field continues to squeeze the trajectory closer to the axis, meanwhile allowing for newly arriving ions (normally, with higher m/z) to enter the C-Trap as well. After ions of all m/z have entered the
Orbitrap analyzer and moved far enough from the outer electrodes, the voltage on the central electrode is kept constant and image current detection takes place.
In the mass analyzer shown in
Figure 1-11 on page 1-14 , stable ion
trajectories combine rotation around an axial central electrode with harmonic oscillations along it. The frequency ω of these harmonic oscillations along the z-axis depends only on the ions’ mass-to-charge ratios m/z and the instrumental constant k:
w
=
z m
×
k
Two split halves of the outer electrode of the Orbitrap analyzer detect the image current produced by the oscillating ions. By Fast Fourier
Transformation (FFT) of the amplified image current, the instrument obtains the frequencies of these axial oscillations and therefore the mass-to-charge ratios of the ions.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-15
Functional Description
Orbitrap Analyzer
Ion Detection
During ion detection, the central electrode and the additional electrode, which deflects ions during injection and compensates electric field imperfections during the measurement (See
are maintained at very stable voltages so that no mass drift can take place. The outer electrode is split in half at z=0, allowing the ion image current in the axial direction to be collected. The image current on each of half of the outer electrode is differentially amplified and then undergoes analog-to-digital conversion before processing using the
Fourier transform algorithm.
Lower m/z Higher m/z
Figure 1-13. Approximate shape of ion packets of different m/z after stabilization of voltages
As mentioned above, stable ion trajectories within the Orbitrap analyzer combine axial oscillations along the z-axis with rotation around the central electrode and vibrations in the radial direction. (See
Figure 1-11 on page 1-14 .) For any given m/z, only the frequency of axial oscillations
is completely independent of initial ion parameters, whereas rotational and radial frequencies exhibit strong dependence on initial radius and energy. Therefore, ions of the same mass-to-charge ratio continue to oscillate along z together, remaining in-phase for many thousands of oscillations.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Orbitrap Analyzer
In contrast to the axial oscillations, the frequencies of radial and rotational motion will vary for ions with slightly different initial parameters. This means that in the radial direction, ions dephase orders of magnitude faster than in the axial direction, and the process occurs in a period of only 50–100 oscillations. After this, the ion packet of a given
m/z assumes the shape of a thin ring, with ions uniformly distributed along its circumference. (See
Figure 1-13 .) Because of this angular and
radial smearing, radial and rotational frequencies cannot appear in the measured spectrum. Meanwhile, axial oscillations will persist, with axial thickness of the ion ring remaining small compared with the axial amplitude. Moving from one half outer electrode to the other, this ring will induce opposite currents on these halves, thus creating a signal to be detected by differential amplification.
Active Temperature Control
Active temperature control is achieved by monitoring temperature directly on the Orbitrap analyzer assembly and compensating any changes in ambient temperature by a thermoelectric cooler (Peltier element) on the outside of the UHV chamber. A dedicated temperature
controller board is used for this purpose. See page 1-62 .
Peltier Cooling
To allow stable operating conditions in the UHV chamber, it can be cooled or heated (outgassing) by means of a Peltier element located on the outside. A second Peltier element is located on the back of the
CE power supply board. See
The Peltier cooling is based on the Peltier Effect, which describes the effect by which the passage of an electric current through a junction of two dissimilar materials (thermoelectric materials) causes temperature differential (cooling effect). The voltage drives the heat to flow from one side of the Peltier element to the other side, resulting in cooling effects on one side and heating effects on the other side.
To remove the heat from the hot side of the Peltier elements, they are connected to the cooling water circuit of the Orbitrap Elite mass spectrometer. See
“Cooling Water Circuit” on page 1-42 for further
information.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-17
Functional Description
HCD Cell
HCD Cell
HCD and ETD
The HCD cell consists of a straight multipole mounted inside a metal tube, which is connected in direct line-of-sight to the C-Trap. It is supplied with collision gas to provide increased gas pressure inside the multipole. See
“Gas Supply” on page 1-37 for details. The ETD Ion
Optic Supply board provides the voltages for the HCD cell. (See
For HCD (Higher Energy Collisional Dissociation), ions are passed through the C-Trap into the HCD cell. The offset between the C-Trap and HCD is used to accelerate the precursor ions into the gas-filled cell.
A potential gradient is applied to the HCD cell to provide fast extraction of ions, such that it returns ions at a reliable rate.
The fragment spectra generated in the HCD cell and detected in the
Orbitrap analyzer show a fragmentation pattern comparable to the pattern of typical triple quadrupole spectra. See the Orbitrap Elite
Getting Started manual for more information.
In the Orbitrap Elite ETD mass spectrometer, ETD reagent anions can efficiently pass through the high pressure region of the HCD cell. This is an important prerequisite to allow for a fast switching (that is, scan to scan) between HCD and ETD fragmentation, thus making comparative measurements possible. When compared with the standard Orbitrap
Elite mass spectrometer, HCD performance is not in any way compromised by the addition of the ETD Module.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
ETD System
Functional Description
ETD System
In the Orbitrap Elite ETD mass spectrometer, an ETD Module is physically coupled to the back of the Orbitrap Elite mass spectrometer.
See
Figure 1-14 . A quadrupole mass filter replaces the octapole of the
Orbitrap Elite MS. See Figure 1-2 on page 1-3 . The linear trap provides
the voltages for the quadrupole mass filter. A tube, which contains the transfer multipole, connects the HCD housing to the ETD Module. See
Figure 1-23 on page 1-30 . The ETD Ion Optic Supply board is
mounted on top of the data acquisition unit on the right side of the instrument. See
ETD Module
Orbitrap
Analyzer
Linear Trap
Thermo Fisher Scientific
Figure 1-14. Orbitrap Elite ETD MS front view
Protein or peptide analyte ions may also be fragmented in the linear trap by negatively charged reagent ions (fluoranthene radical anions) from the reagent ion source (ETD Module). These negatively charged ions transfer electrons to protein or peptide analyte ions and cause them to fragment at their peptide bonds to produce c and z type fragments
(versus the y and b fragments produced by CID). The resulting analyte fragment ions provide another way of analyzing these molecules as compared to CID and PQD. Electron Transfer Dissociation (ETD) improves the identification of important post-translational modification
(PTM) for characterization.
Note Among others, the ETD system is also available as an upgrade on existing Velos Pro and Orbitrap Elite systems.
▲
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-19
Electrospray Ion Source S-Lens
Velos Pro MS
Square Quadrupole with neutral blocking
Octopole
Orbitrap analyzer
High Pressure Cell Low Pressure Cell Quadrupole Mass Filter C-Trap HCD Cell
ETD System
Transfer Multipole Reagent Ion Source e
-
High-field Orbitrap Mass Analyzer
Reagent 1
Heated Inlet
Reagent 2
Heated Inlet
Figure 1-15. Schematic of the Orbitrap Elite ETD MS
1-20
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Principle of Operation
Functional Description
ETD System
During a typical ETD MS/MS scan, analyte cations are injected into the linear trap for subsequent precursor cation isolation. Then,
ETD reagent anions are generated in the CI ion source and are transferred into the linear trap via RF-only ion guides (transfer multipoles), the gas-filled HCD cell, and the C-Trap. (See
The reagent ions pass a quadrupole mass filter between C-Trap and linear trap. This ion guide works as a low pass mass filter to remove the adduct ions of the fluoranthene radicals and molecular nitrogen at
m/z 216. These adduct ions favor proton transfer reactions instead of electron transfer.
The application of a supplementary RF voltage on the end lenses of the linear trap allows ions of opposite polarity to be trapped in the same space at the same time (charge-sign independent trapping—CSIT).
During ion-ion reactions in the linear trap, electrons are transferred from the reagent anions to the precursor cations. The resulting product ions are mass-to-charge (m/z) analyzed in either the linear trap (if speed and sensitivity are important) or the Orbitrap analyzer (if mass resolution and mass accuracy are important).
ETD Module
Figure 1-16 on page 1-22 shows the rear side of the ETD Module. It
consists of the reagent ion source, ETD Module electronics,
ETD Module power supply, ETD Module forepump, and the hardware that connects the ETD Module to the mass detectors.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-21
Functional Description
ETD System
Fan filter
Control elements of inlet valve
Inlet port for ETD reagent carrier gas
Figure 1-16. Orbitrap Elite ETD MS, rear side
Cabinet for
ETD forepump
15 14
Labeled components: 1=power module, 2=connector to Interface Board
(Interface Board is behind the ETD Control PCB, item #4), 3=DC HV Supply
PCB, 4=ETD Control PCB, 5=Heater Control PCB, 6=ion gauge,7=inlet valve solenoid, 8=inlet valve lever in closed (down) position, 9=reagent heater 1,
10=reagent heater 2, 11=ion volume tool handle, 12=guide bar, 13=TMP,
14=Convectron™ gauge, 15=vacuum manifold (contains ion source and ion volume)
Figure 1-17. Rear view of the ETD Module, with major component locations
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
ETD System
Orbitrap Elite
ETD Module
Peripherals
Power Outlet
Orbitrap Analyzer
Instrument Control Board
Orbitrap Vacuum System,
ETD Forepump
Power Module
Interface
Board
ETD Control PCB
DC HV Supply PCB
Heater Control PCB
ETD TMP
4
Ion Gauge
Ion Source
H1
H2
Reagent
Heaters
3
Flow
Control
Convectron Gauge
ETD Reagent
Carrier Gas
3 = Transfer line
4 = Ion volume
Figure 1-18. ETD Module functional block diagram
The following sections describe the major ETD Module components
that are shown in Figure 1-17 on page 1-22
ETD Power Module
ETD Ion Optic
Supply Board
The ETD power module (item #1 in Figure 1-17 ) receives 220 V, 10 A,
from the peripherals power outlet. See
distributes appropriate voltages and currents to the ETD components. It also contains DC power supplies.
ETD Module Power Panel
The external receptacles and switches for the power module are located on the ETD power module panel at the right side of the ETD Module.
See
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-23
Functional Description
ETD System
ETD Module
ETD Module power panel
Figure 1-19. Right side of the Orbitrap Elite ETD MS
shows a close up picture of the ETD Power Module panel.
Power In is connected to the peripherals power outlet of the
MS portion. See
Figure 1-9 on page 1-10 . Forepump is a receptacle to
power the ETD forepump (220 V AC, 5 A).
ETD Module Service switch
ETD Module Power switch
ETD Module Forepump receptacle
ETD Module Power receptacle
ETD Module Power Panel
Figure 1-20. ETD Power Module panel
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
ETD Module Interface Board
Functional Description
ETD System
The ETD power module panel contains the main breaker and the service switch for the ETD Module. During normal operation, the
ETD Power switch is left On and the service switch is left in the
Operating Mode position. As a safety feature, both components of the
Orbitrap Elite ETD system (the mass spectrometer and the
ETD Module) are shut down with one set of switches, the mass spectrometer switches. When you perform maintenance on components
inside the ETD Module as described in “Maintenance of the ETD
, you set the mass spectrometer’s service switch to the Service position. The service switch turns On or Off power to all
ETD Module components except turbomolecular pump and forepump.
The ETD Module Interface board (item #2 in
) provides an electronic interface between the ETD Module and the MS. This board also allows the power to both the MS and the
ETD Module to be controlled by the MS power control panel switches:
•
The MS Main Power switch controls the power supply to all components in both the MS and the ETD Module.
•
The MS FT Electronics switch controls the power supply to all mass spectrometer and ETD Module components except the pumps that are connected to the MS and the ETD Module.
Note The ability to control the power to both components of the
Orbitrap Elite ETD mass spectrometer at one point (the power control panel switches of the MS) is a safety feature.
▲
ETD Control PCB
Thermo Fisher Scientific
The ETD Control PCB (item #4 in Figure 1-17
) controls most of the
ETD Module functions. The ETD Control PCB consists of circuits that control:
•
ETD Module operating logic
•
Ion source (filament, ion source heater, lenses)
•
Heater temperature and readback logic (for reagent heaters, transfer line heater, and the restrictor oven heater)
•
Reagent gas flow
•
Oven cooling gas control
•
Ion gauge
•
Convectron™ gauge
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-25
Functional Description
ETD System
The DC HV Supply PCB (item #3 in Figure 1-17 on page 1-22
) is plugged in to the ETD Control PCB.
ETD Heater Control PCB
The ETD Module Heater Control PCB (item #6 in
contains the power source and temperature sensing circuitry for the four heaters in the reagent ion source. The heaters are H1 and H2 (the two reagent heaters,
Figure 1-18 on page 1-23 , and items #9 and #10 in
), the transfer line heater (#3 in
restrictor oven heater (not shown in
Figure 1-17 ). The Heater Control
PCB reports temperature information to the heater temperature and readback logic on the ETD Control PCB. The heater temperature and readback logic controls how the Heater Control PCB applies power to the ETD Module heaters.
Reagent Carrier Gas Flow Control for ETD
The ETD Module contains a digital flow control for the chemical ionization (CI) gas/reagent carrier gas provided by the ETD Control
PCB (See
Figure 1-17 on page 1-22 .) and an electronic pressure
regulator. The gas serves two functions in the ETD Module:
•
As a carrier gas, the nitrogen sweeps the reagent (fluoranthene) from the vial to the ion source where the reagent radical anions are formed.
•
As a chemical ionization (CI) vehicle, the nitrogen undergoes collisions with 70 eV electrons from the filament in the ion volume.
These 70 eV electrons from the filament knock electrons off of the nitrogen molecules (nitrogen ions are created). The secondary electrons resulting from these collisions have near thermal kinetic energies. These thermal electrons are captured by the fluoranthene to form reagent radical anions that react with the analyte.
Thermo Fisher Scientific strongly recommends a mixture of
25% helium and 75% nitrogen. The helium in this mixture serves as a tracer gas to enable leak checking of gas connections using conventional thermal conductivity-based leak detectors, which are widely used to
check leaks in gas chromatography equipment. See “Gas Supply of the
Reagent Ion Source” on page 1-40
for detailed information.
The reagent carrier gas supply in the laboratory is connected to the
ETD reagent carrier gas port at the rear side of the ETD Module. See
.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Reagent Heaters
Functional Description
ETD System
The reagent heaters (items #9 and #10 in
and H2 in Figure 1-18 on page 1-23
) heat the reagent to obtain a sufficient amount of reagent vapor in the carrier gas. The reagent heaters are powered by the Heater Control PCB which, in turn, is controlled by the ETD Control PCB.
The reagent heaters are turned on by selecting the Reagent Ion Source
On check box in the Reagent Ion Source dialog box. See
.
Thermo Fisher Scientific
Figure 1-21. Reagent Ion Source dialog box
When you deselect the Reagent Ion Source On check box, the reagent heaters and filament immediately turn off and the reagent ion source goes into Standby mode.
When the reagent ion source is placed in Off mode, cooling nitrogen
(high-purity nitrogen) will turn on. This is confirmed by an audible rush (hissing noise) of nitrogen from the reagent ion source area in the back of the ETD Module. This is normal operation. See also
Off the Reagent Ion Source: What to Expect” on page 2-14
.
Warning
Burn Hazard. When the reagent ion source is in Off mode, restrictor oven, transfer line, and ion source remain at 160 °C.
▲
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-27
Functional Description
ETD System
The nitrogen cooling gas turns off when the reagent heaters reach 70 °C.
If it is necessary to install or replace the reagent vials, follow the
procedure in “Replacing the Reagent Vials” on page 3-48
.
Note The rushing or hissing noise of the nitrogen coming from the back of the ETD Module will stop when the cooling nitrogen turns off.
▲
On
Reagent Ion Source
Off
Warning
Burn Hazard. Do not attempt to handle the vials or vial holders when the cooling nitrogen stops. They are still too hot to handle when the cooling nitrogen stops at a vial temperature of 70 °C. Follow
the procedures in “Replacing the Reagent Vials” on page 3-48 if it is
necessary to install or replace the reagent vials.
▲
When the reagent heaters are in Standby mode, they are immediately turned on by selecting the Reagent Ion Source On check box
(
Figure 1-21 on page 1-27 ). When starting from room temperature, it
takes up to ten minutes for the reagent heaters and vials to stabilize at
108 °C and reagent to delivered to the ion source.
Note When you switch on a cold reagent ion source, the Tune Plus software warns you that the vial temperature is not sufficient. The filament is automatically switched on after the temperature has stabilized.
▲
Standby
When you click the Standby button in the Tune Plus window (shown in the left margin), you initiate a Standby process. It delays turning off the reagent heaters and the start of nitrogen cooling for one hour after the system is placed in Standby. This method of placing the system in
Standby permits a quick return to operation after a break (lasting one hour or less) rather than waiting up to ten minutes for the heaters to return to temperature and reagent delivery to be fully restored.
In summary:
•
The reagent heaters turn off immediately when the Reagent Ion
Source On check box is deselected in the Reagent Ion Source dialog
box. (See Figure 1-21 on page 1-27
.)
•
The reagent heaters turn off one hour after the system is placed in
Standby by clicking the Standby button in the Tune Plus window.
The ion source ( Figure 1-17 on page 1-22
and inside of the vacuum manifold, see item #14 in
Figure 1-17 ) is where the reagent ions are
formed. The ion source contains the filament, the reagent ion volume, and the ion source heater. The filament is the source of electrons that
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
ETD System react with the reagent to form reagent ions. The reagent ion volume is the space where this reaction takes place. See
source heater is controlled by the ETD Control PCB.
Filament
Changeable Ion Volume
Heated Transfer Line
Fused Silica
Heated Dual Restrictor
Enclosure
Thermo Fisher Scientific
Figure 1-22. Reagent ion source schematics
The reagent ion source contains two reagent vials, CI/carrier gas
(nitrogen) handling hardware and flow restrictors, ion volume and filament, ion optics, and heaters for these components. The flow restrictors keep the internal pressure of the reagent vials below atmospheric pressure. This prevents the contents of the reagent vials from being expelled to the laboratory atmosphere.
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-29
Functional Description
Vacuum System
Vacuum System
HCD Housing
shows a schematical overview of the Orbitrap analyzer vacuum system.
UHV Chamber Vacuum Chamber
Linear Trap
ETD Module
Vacuum System, see
TMP 4
Pirani Gauge
TMP 2
Cold Ion Gauge
TMP 3 TMP 1
Forepump Forepump
Figure 1-23. Schematic of Orbitrap analyzer vacuum system (CLT compartment and Orbitrap chamber not shown) a a
For an abridged version of the parts list, see page 4-3
.
The Orbitrap Elite mass spectrometer has the following vacuum compartments:
•
CLT compartment in the aluminum vacuum chamber (pumped by the same pump as the linear trap)
•
Vacuum chamber (pumped by a water-cooled 60 L/s—for
N
2
—turbomolecular pump HiPace™ 80, TMP 1, manufacturer:
Pfeiffer)
•
Ultra high vacuum chamber (UHV chamber, pumped by a water-cooled 60 L/s turbomolecular pump HiPace 80, TMP 2, manufacturer: Pfeiffer)
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Vacuum System
•
Orbitrap chamber (pumped by a 260 L/s—for N
2
—water-cooled turbomolecular pump HiPace 300, TMP 3, manufacturer: Pfeiffer)
•
HCD housing (pumped by a water-cooled 60 L/s turbomolecular pump HiPace 80, TMP 4, manufacturer: Pfeiffer)
The forepumps of the linear trap provide the forevacuum for the turbomolecular pumps TMP 1 to TMP 4.
Turbomolecular Pumps
All parts of the system except for the Orbitrap analyzer are mounted in a aluminum vacuum chamber that is evacuated by a 60 L/s turbomolecular pump (TMP 1, see
). The rotary vane
pumps of the linear trap (see page 1-33
) provide the forevacuum for this pump. This chamber is bolted to a stainless steel welded UHV chamber, which accommodates Orbitrap analyzer, lenses, and corresponding electrical connections.
TMP 4 TMP 1
Thermo Fisher Scientific
Gas Regulator for Vent Valve
Pirani Gauge
Figure 1-24. Vacuum components on the left instrument side
The UHV chamber is evacuated down to 10
-8 mbar pressure range by a
60 L/s UHV turbomolecular pump (TMP 2, see Figure 1-25 on page 1-32
). The Orbitrap analyzer itself is separated from the
UHV chamber by differential apertures and is evacuated down to
10
-10 mbar by a 260 L/s turbomolecular pump (TMP 3, see
Figure 1-25 ). The HCD housing is evacuated by a 60 L/s
UHV turbomolecular pump (TMP 4, see Figure 1-24 ) that is mounted
to its bottom via an elbow. This dedicated pump for the HCD cell protects the low pressure cell of the linear trap from gas overload.
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-31
Functional Description
Vacuum System
In the Orbitrap Elite ETD mass spectrometer, a tube that contains the transfer multipole (flatapole) connects the HCD housing to the
ETD Module.
Preamplifier
HCD housing
Cooling water supplies for
Peltier element and Preamplifier
Cold ion gauge
TMP 3
TMP 2
Figure 1-25. Vacuum components on the right instrument side
All turbomolecular pumps are equipped with TC 110 control units
(manufacturer: Pfeiffer). A 24 V switch mode power supply provides the electric power for all four turbomolecular pumps of the system.
Linear Trap Turbomolecular Pump
A separate turbomolecular pump provides the high vacuum for the linear ion trap. It it is mounted to the bottom of the vacuum manifold of the linear ion trap. For more information, refer to the LTQ Series
Hardware Manual.
ETD Module Turbomolecular Pump
In the Orbitrap Elite ETD mass spectrometer, a dedicated turbomolecular pump (Edwards EXT75DX) provides the high vacuum for the ETD reagent ion source. See
Figure 1-17 on page 1-22 . It is
backed up by a dedicated rotary vane pump at the bottom of the
ETD Module. See
Figure 1-27 on page 1-34 . This air-cooled
turbomolecular pump contains no user-serviceable parts.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Vacuum System
Forepumps of the Linear Trap
The rotary vane pumps from the linear trap serve as forepumps for the three smaller turbomolecular pumps (TMP 1, TMP 2, and TMP 4).
The exhaust hose from the forepumps is led to the back of the instrument and connects them to the exhaust system in the laboratory.
The forepumps are located on a small cart in the forepumps cabinet below the linear trap. See
Oil mist filters
Thermo Fisher Scientific
Forepumps
Figure 1-26. Forepumps cabinet
To minimize the ingress of pump oil into the exhaust system, the outlets
of the forepumps are fitted to oil mist filters. See page 3-11
on instructions about returning the collected oil to the forepumps.
The forepumps of the linear trap are powered by the power panel of the linear ion trap.
Warning
Health Hazard. Hazardous materials may be present in the effluent of the forepumps. The connection to an adequate exhaust system is mandatory!
▲
Leave the switches of the forepumps always in the On position to provide the control from the vacuum control panel. Before starting the pumps, however, make sure that:
•
The forevacuum pumps are filled with oil,
•
They are connected to the power supply, and
•
The gas ballast is shut.
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-33
Functional Description
Vacuum System
For a detailed description of the forepumps, refer to the handbook of the manufacturer.
Forepump of the ETD Module
In the Orbitrap Elite ETD mass spectrometer, a rotary vane pump
(Edwards RV 3) provides the forevacuum for the ETD turbomolecular
pump. (See “ETD Module Turbomolecular Pump” on page 1-32
.) It is located in a cabinet at the bottom of the ETD Module. The
ETD forepump is equipped with an oil mist filter and stands on a drip
pan. See Figure 1-27 on page 1-34
.
An exhaust hose connects the forepump to the exhaust system in the laboratory. A forevacuum tube connects the ETD forepump to the
ETD TMP. The forepump electrical cord is plugged into the Forepump
receptacle on the ETD Module power panel. See Figure 1-20 on page 1-24 .
For maintenance instructions for the ETD forepump, see
“Maintenance of the ETD Forepump” on page 3-5 and the manual that came with the
forepump.
Exhaust hose
Forevacuum tube
Oil mist filter
Drip pan
Forepump electrical cord
Figure 1-27. Forepump for ETD Module
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Vacuum System
Vacuum System Controls
The power distribution board controls all turbomolecular pumps via voltage levels. See
“Power Distribution Board” on page 1-53 . An
interface for RS485 data via the instrument control board connects the
turbomolecular pumps with the linear ion trap. (See “Instrument
Control Board” on page 1-52 .) The turbomolecular pump of the linear
ion trap and the ETD turbomolecular pump have individual controllers.
Vacuum Gauges
Several vacuum gauges monitor the vacuum within the instrument:
•
The forevacuum of the Orbitrap Elite mass spectrometer is monitored by an Active Pirani gauge (TPR 280, manufacturer:
Pfeiffer) connected to the forevacuum line. See
.
•
The high vacuum of the Orbitrap Elite mass spectrometer is monitored by a Cold Ion Gauge (IKR 270, manufacturer: Pfeiffer) connected to the UHV chamber. See
Because the gauge would be contaminated at higher pressures, it is turned on only when the forevacuum has fallen below a safety threshold (<10
-2 mbar).
•
The linear ion trap vacuum is monitored by a Convectron™ gauge and an ion gauge. Refer to the LTQ Series Hardware Manual for more information.
•
In the Orbitrap Elite ETD mass spectrometer, two dedicated vacuum gauges monitor the vacuum in the ETD Module. A
Convectron gauge (see
Figure 1-18 on page 1-23 ) monitors the pressure in the ETD forevacuum line
and an ion gauge (see
Figure 1-17 ) monitors the pressure in the
reagent ion source.
shows typical pressure readings in the
ETD Module.
Table 1-3. Typical pressure readings in the ETD Module
Conditions Convection Gauge Reading
CI gas pressure set to 20 psi 0.1–0.01 Torr
Ion Gauge Reading
20–35×10
-5
Torr
The vacuum gauges of the Orbitrap Elite mass spectrometer are connected to the power distribution board that directly responds to the
pressure values. (See “Power Distribution Board” on page 1-53 .) The
analog values are digitized by the instrument control board. (See
“Instrument Control Board” on page 1-52 .) They are then sent as
readout values to the data system.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-35
Functional Description
Vacuum System
Switching on the Vacuum System
When the vacuum system is switched on, the following occurs:
1. After the Vacuum Pumps switch is switched On, the pumps of the linear ion trap and the Orbitrap Elite mass spectrometer are run up.
The Pirani gauge (see above) controls the Orbitrap Elite MS low vacuum pressure as well as the pressure at the forevacuum pumps.
Within a short time, a significant pressure decrease must be observed. The goodness of the vacuum can be estimated by means of the rotation speed of the turbomolecular pumps (for example,
80% after 15 minutes).
2. If the working pressure is not reached after the preset time, the complete system is switched off. At the status LED panel of the power distribution board, an error message (Vacuum Failure) is put out (see below).
3. The Cold Ion Gauge is only switched on after the low vacuum is reached. It is then used to monitor the vacuum in the Orbitrap analyzer region.
Vacuum Failure
In case the pressure in the Orbitrap Elite mass spectrometer or the linear ion trap exceeds a safety threshold, the complete system including linear ion trap, electronics, and pumps is switched off. However, the power distribution is kept under current and puts out an error message at the
LED panel. (See “Power Distribution Board” on page 1-53 .) It can be
reset by switching the main power switch off and on. (See
Upon venting, the vent valves of the turbomolecular pumps on the
Orbitrap analyzer stay closed. Only the vent valve of the linear ion trap
is used. (See “Vent Valve of the Linear Ion Trap” on page 1-39 .)
Vacuum System Heating during a System Bakeout
After the system has been open to the atmosphere (for example, for maintenance work), the vacuum deteriorates due to contaminations of the inner parts of the vacuum system caused by moisture or a power outage. These contaminations must be removed by heating the vacuum system: a system bakeout. See
“Baking Out the System” on page 3-4
.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Gas Supply
Gas Supply
This section describes the gas supplies for the mass analyzers of the
Orbitrap Elite mass spectrometer and the reagent ion source of the
Orbitrap Elite ETD mass spectrometer.
Gas Supply for the Mass Analyzers
ETD reagent carrier gas
Figure 1-28 shows a schematical view of the gas supply for the
instrument. The gas supply of the ETD system is highlighted in gray.
Helium (He)
1
Nitrogen (N
2
)
ETD Module Linear Trap
Regulator with manometer
Analyzer
TMP 2
TMP 3
C-Trap
TMP 1
Cooling Gas
N
2
venting TMP 4
Figure 1-28. Schematic of gas supply for Orbitrap Elite ETD MS a a
For parts lists of the gas supply, see page 4-4 and
HCD Cell
Gas Inlet Ports of the Instrument
Vent valve
On its right side (See Figure 1-9 on page 1-10 .), the instrument provides
three gas inlet ports for the gas supply of the mass analyzers:
•
Nitrogen: The linear trap requires high-purity (99%) nitrogen for the API sheath gas and auxiliary/sweep gas. The required gas pressure is 690 ± 140 kPa (6.9 ± 1.4 bar, 100 ± 20 psi).
Thermo Fisher Scientific
1
The port named Collision Gas is not used in the Orbitrap Elite MS.
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-37
Functional Description
Gas Supply
In the Orbitrap Elite ETD mass spectrometer, the ETD system uses the high-purity nitrogen for cooling the reagent vials when the reagent ion source is turned off.
•
Helium: The linear trap requires ultra-high purity (99.999%) helium for the collision gas. The required gas pressure is
275 ± 70 kPa (2.75 ± 0.7 bar, 40 ±10 psi).
Warning
Danger of Asphyxiation. Accumulation of nitrogen gas could displace sufficient oxygen to suffocate personnel in the laboratory.
Ensure that the laboratory is well ventilated.
▲
❖
To connect the nitrogen source to the Orbitrap Elite mass spectrometer
1. Connect an appropriate length of Teflon™ tubing to the nitrogen source in the laboratory. The Installation Kit contains 6 m (20 ft) of suitable Teflon tubing (OD 6 mm, P/N 0690280). The connection for the Teflon hose to the nitrogen gas supply is not provided in the kit; you have to supply this part.
2. Connect the opposite end of the Teflon tubing to the press-in fitting labeled Nitrogen, which is located at the right side of the
instrument. See Figure 1-9 on page 1-10
. To connect the tubing, align the Teflon tubing with the opening in the fitting and firmly push the tubing into the fitting until the tubing is secure.
❖
To connect the helium source to the Orbitrap Elite mass spectrometer
1. Connect an appropriate length of 1/8-in. ID copper or stainless steel tubing with a brass Swagelok-type 1/8-in. nut (P/N 00101-15500) and a 2-piece brass 1/8-in. ID ferrule [P/N 00101-08500 (front),
P/N 00101-2500 (back)] to the Helium gas inlet. See
for the proper orientation of the fitting and ferrule.
2. Connect the opposite end of the tubing to the helium gas source, using an appropriate fitting.
Gas hose Front ferrule
Swagelok-type nut Back ferrule
Figure 1-29. Proper orientation of the Swagelok-type nut and two-piece ferrule
1-38
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Gas Supply
Gas Distribution Within the Instrument
Helium gas is led from the helium port through a stainless steel capillary to the right rear side of the linear trap. See
High purity nitrogen gas is led from the nitrogen port via Teflon tubing to the right side of the Orbitrap Elite mass spectrometer. Here, two
T-pieces divide the nitrogen gas flow into three parts.
The first part of the high purity nitrogen gas flow is directed through
Teflon tubing via a pressure regulator to the vent valve of the linear trap.
(See
.) The second part of the nitrogen flow is directed through Teflon tubing to the API source. The third part of the nitrogen flow enters a gas regulator with manometer, which keeps the gas pressure to the C-Trap and HCD cell constant. (See
background.) From the regulator, the collision gas is led through red
PEEKSil™ tubing (100 mm ID silica capillary in 1/16 inch
PEEK tubing) to the collision octapole next to the curved linear trap
(flow rate: ~0.5 mL/min). The nitrogen gas leaking from the
HCD collision cell (3–5 mbar) is used for ion trapping and cooling in the C-Trap.
Gas for HCD and C-Trap
Gas for Vent Valve
Figure 1-30. Gas regulators
In the Orbitrap Elite ETD mass spectrometer, nitrogen is also directed through Teflon tubing to the ETD Module to be used for cooling the reagent vials when the reagent ion source is turned off.
Vent Valve of the Linear Ion Trap
Thermo Fisher Scientific
If the system and pumps are switched off, the system is vented. The vent valve is controlled by the linear ion trap. The LTQ Series Hardware
Manual contains further information about the vent valve.
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-39
Functional Description
Gas Supply
The instrument is vented with high purity nitrogen from the same tubing that supplies the Velos Pro MS sheath gas. See
Figure 1-28 on page 1-37 . The vent valve of the Velos Pro mass spectrometer is supplied
via a pressure regulator that is set to a venting pressure of 3–4 psi. The pressure regulator is located at the left side of the Orbitrap Elite mass
spectrometer. (See Figure 1-30
, front.)
Gas Supply of the Reagent Ion Source
In addition to high purity nitrogen for cooling, the reagent ion source of the Orbitrap Elite ETD mass spectrometer uses a mixture of 25% helium and 75% nitrogen gas as carrier gas and chemical ionization (CI) vehicle. This gas mixture must be ultra high-purity (minimum purity
99.999%) with less than 3.0 ppm each of water, oxygen, and total hydrocarbons. The required gas pressure is 690 ± 140 kPa
(6.9 ± 1.4 bar, 100 ± 20 psi). The ETD carrier gas supply of the laboratory is connected via metal tubing to the inlet port at the rear side
of the instrument. See Figure 1-31
.
Figure 1-31. ETD reagent carrier gas port at the ETD Module
The helium in this mixture serves as a tracer gas to enable leak checking of gas connections using conventional thermal conductivity-based leak detectors, which are widely used to check leaks in gas chromatography equipment.
Note If the helium/nitrogen mixture is not available, then use a nitrogen supply that is ultra high-purity (99.999%) with less than 3.0 ppm each of water, oxygen, and total hydrocarbons.
▲
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Triple Gas Filter
Functional Description
Gas Supply
A triple (oxygen/water/hydrogen) gas filter is installed between the regulator on the reagent carrier gas source and the ETD module to ensure that the reagent carrier gas (either nitrogen or helium/nitrogen) is better than 99.999% pure with much less than 1 ppm of oxygen, water, and hydrocarbons.
Refer to the filter manufacturer’s instructions for information about how to monitor the color changes in the filters that indicated when the filters need to be replaced, as well as information about where to order new filters. If there are no leaks in the reagent carrier gas plumbing, you can expect the filters to last a year or more. Thermo Fisher Scientific strongly recommends that a Thermo Fisher Scientific field service engineer replace the gas filters.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-41
Functional Description
Cooling Water Circuit
Cooling Water Circuit
shows a schematical view of the cooling water circuit in the Orbitrap Elite mass spectrometer. For a parts list of the cooling water circuit, see
page 4-4 . Cooling water at a temperature of
20 °C enters and leaves the instrument at the bottom of the right side.
. First, the fresh water passes through the turbomolecular pumps in the order TMP 3 → TMP 1 → TMP 4 →
TMP 2. Then it passes through the heating element (Peltier element) that maintains (±0.5 °C) the preset temperature of the analyzer. After that, the cooling water passes through the preamplifier cooling unit.
Before it leaves the instrument, the water passes through the other
Peltier element at the back of the central electrode power supply board.
A flow control sensor is connected to the power distribution board and allows displaying the current flow rate of the cooling water in the software. An inline filter, which is installed upstream, protects the
sensor. It must be replaced annually, see page 3-58
for instructions.
Recirculating chiller
Power Distribution Board
Linear Trap
Water filter
Flow control sensor
Peltier element Central Electrode
Power Supply Box
TMP 4
TMP 2
Figure 1-32. Schematic of cooling water circuit
Water cooler for TMP
1-42
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
Preamplifier cooling
Heating element
(Peltier element)
TMP 3
Analyzer
TMP 1
Thermo Fisher Scientific
Functional Description
Cooling Water Circuit
Recirculating Chiller
A recirculating chiller (Thermo Scientific NESLAB ThermoFlex™ 900) is shipped with the instrument, making the mass spectrometer independent from any cooling water supply. A wall receptacle provides the electric power for the chiller. Two water hoses (black), internal diameter 9 mm, wall thickness 3 mm, length approx. 3 m (~10 ft) are shipped with the instrument.
For instruction about performing maintenance for the chiller, see
“Maintenance of the Cooling Circuit” on page 3-58
. See also the manufacturer’s manual for the chiller.
Properties of Cooling Water
The water temperature is not critical, but should be in the range of 20 to
25 °C (68 to 77 °F). Lower temperatures could lead to a condensation of atmospheric water vapor. It is recommended to use distilled water rather than de-ionized water due to lower concentration of bacteria and residual organic matter.
The water should be free of suspended matter to avoid clogging of the cooling circuit. In special cases, an in-line filter is recommended to guarantee consistent water quality.
The cooling water should meet the following requirements:
Hardness:
Resistivity:
Total dissolved solids: pH:
<0.05 ppm
1–3 MΩ/cm
<10 ppm
7–8
Warning
Burn Hazard. If the water circuit fails, all parts of the water distribution unit may be considerably heated up. Do not touch the parts! Before disconnecting the cooling water hoses, make sure the cooling water has cooled down!
▲
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-43
Functional Description
Printed Circuit Boards
Printed Circuit Boards
The Orbitrap Elite mass spectrometer is controlled by a PC running the
Xcalibur™ software suite. The software controls all aspects of the instrument. The main software elements are the communication with the linear ion trap, the control of ion detection, and the control of the
Orbitrap analyzer.
The following pages contain a short overview of the electronic boards in the MS portion of the Orbitrap Elite mass spectrometer. For each board, its respective location and function are given. If applicable, the diagnostic LEDs on the board are described. For a description of the
printed circuit boards in the ETD Module, see “ETD Module” on page 1-21 .
The electronics of the Orbitrap Elite mass spectrometer contains complicated and numerous circuits. Therefore, only qualified and skilled electronics engineers should perform servicing.
A Thermo Fisher Scientific field service engineer should be called if servicing is required. It is further recommended to use Thermo Fisher
Scientific spare parts only. When replacing fuses, only use the correct type. Before calling a service engineer, please try to localize the defect via errors indicated in the software or diagnostics. A precise description of the defect will ease the repair and reduce the costs.
Warning
Electrical Shock Hazard. Parts of the printed circuit boards are at high voltage. Shut down the instrument and disconnect it from line power before performing service. Opening the electronics cabinet is only allowed for maintenance purposes by qualified personnel.
▲
Note Many of the electronic components can be tested by the Orbitrap
Elite MS diagnostics, which is accessible from the Tune Plus window.
▲
1-44
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Printed Circuit Boards
Linear Ion Trap Electronics
The linear ion trap is connected to the Orbitrap Elite MS main power
switch. The linear ion trap has a sheet metal back cover. Figure 1-33
shows the electronic connections at the rear side of the linear trap.
Cold Ion Gauge
Figure 1-33. Electronic connections to linear trap
The linear ion trap electronics has two connections with the Orbitrap
Elite MS electronics:
•
Data communication with the internal computer of the Orbitrap
Elite mass spectrometer. See
“Electronic Boards on the Right Side of the Instrument” on page 1-46 .
•
Signal communication (SPI bus) with supply information for the instrument control board. See
“Instrument Control Board” on page 1-52
.
For further information about the linear ion trap electronics, refer to the
LTQ Series Hardware Manual.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-45
Functional Description
Printed Circuit Boards
Electronic Boards on the Right Side of the Instrument
shows the parts of the instrument when the right side panel is opened. A transparent cover protects the lower part.
ETD Ion Optic Supply board housing
Preamplifier
Computer housing
(data acquisition unit)
Instrument Control board housing
Power Supply 1 board
Power Distribution board
Ground wire for side panel
Figure 1-34. Electronic boards on the right side of the Orbitrap Elite MS
The side panel is connected to the instrument frame by two
green/yellow ground wires. See bottom of Figure 1-34
. The connectors on the panel are labeled with green-yellow PE (for Protective Earth) signs. See photo left. Do not forget to reconnect them before closing the panel!
ETD Ion Optic Supply Board
The ETD Ion Optic Supply board is mounted on top of the data acquisition unit. See
Figure 1-35 . It supplies the voltages for the
HCD cell. In the Orbitrap Elite ETD mass spectrometer, this board also supplies the RF voltage and the DC voltages for the ETD Module: an
RF voltage with DC offset, three DC voltages with ±250 V, and a
DC voltage with ±12 V.
1-46
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Printed Circuit Boards
Thermo Fisher Scientific
Figure 1-35. ETD Ion Optic Supply board
The diagnostic LEDs on the ETD ion optic supply board are listed in
. The positions of the diagnostic LEDs on the
board are indicated by white rectangles in Figure 1-36
.
Table 1-4. Diagnostic LEDs on the ETD Ion Optic Supply board
No.
Name Color Description
LD1 +275 V
LD2 -275 V
Green +275 V input voltage present
Green -275 V input voltage present
LD3 RF Supply Green RF input voltage (22 V) present
LD4 +24 V Green +24 V input voltage present
LD5 +15 V
LD6 -15 V
LD7 RF1_ON
Green
Green
Blue
+15 V input voltage present
-15 V input voltage present
RF-generator switched on
Normal Operating
Condition
On
On
On
On
On
On
On/Off, depending on active application
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-47
Functional Description
Printed Circuit Boards
Preamplifier
The preamplifier is located in a housing next to the Cold Ion Gauge. See
. It is water cooled to protect it during a system bakeout.
Cooling water supply for Preamplifier
Cooling water supply for Peltier element
Cold Ion Gauge
Figure 1-36. Preamplifier
This board is a broadband preamplifier with differential high-impedance inputs. It serves as a detection amplifier and impedance converter for the image current created by the oscillating ions. The output current is transferred to the data acquisition board. It has an amplification factor of about 60 dB and covers the frequency range from
15 kHz to 10 MHz.
The diagnostic LEDs on the preamplifier are listed in
Table 1-5 on page 1-49 . The positions of the diagnostic LEDs on the board are
indicated by white rectangles in
1-48
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Internal Computer
Functional Description
Printed Circuit Boards
Table 1-5. Diagnostic LEDs on the Preamplifier board
No.
Name Color Description
LD1 Overload Yellow RF output is overloaded
LD2 +5 V Green +5 V input voltage present
LD3 +15 V
LD4 -5 V
Green
Green
+15 V input voltage present
-5 V input voltage present
LD5 Input off Yellow RF inputs are shortened
(protection)
Normal Operating
Condition
Off
On
On
On
On, off during
Detect
Figure 1-37 shows the components of the data acquisition unit. The
unit is mounted in a housing located at the right side of the instrument.
ETD Ion Optic Supply board housing
Computer housing with
Data Acquisition Analog board, Data Acquisition
Digital PCI board, and
Power Supply 2 board
Thermo Fisher Scientific
Instrument Control board housing
Figure 1-37. Data Acquisition unit
The internal computer contains a computer mainboard with an
ATX power supply. The data acquisition digital PCI board is directly plugged into the mainboard. The data acquisition analog board is mounted on top of the computer mainboard.
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-49
Functional Description
Printed Circuit Boards
Data Acquisition Digital PCI Board
shows the data acquisition digital PCI board. It is an add-on board to the internal computer. (See
Figure 1-38. Data Acquisition Digital PCI board
This board is used to convert detected ion signals to digital form and to interface to the computer mainboard. The board has two 16 bit parallel connections to the DAC and the ADC on the data acquisition analog board, which are used for controlling and reading-back signals. A high-speed link port channel is also on the board that is used to communicate with the electronics in the linear ion trap.
Precision timing is derived from the data acquisition analog board and events with lower requirements use the timer in the internal computer.
This timer is used to check at regular intervals whether the foreground process works as expected.
Communication takes place not only between the linear ion trap and the internal computer of the Orbitrap Elite system, but also between the linear ion trap and the data system computer. For further information about the data system, refer to the LTQ Series Hardware Manual.
The diagnostic LEDs listed in Table 1-6
show the status of the board.
The position of the LEDs on the board is indicated by a red rectangle in
.
Table 1-6. Diagnostic LEDs of the Data Acquisition Digital PCI board
Name Color
+5 V Green
+3.3 V Green
+2.5 V Green
Description
+5 V voltage present
+3.3 V voltage present
+2.5 V voltage present
Normal Operating Condition
On
On
On
1-50
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Printed Circuit Boards
Data Acquisition Analog Board
Figure 1-39 shows the data acquisition analog board. This board is an
add-on board to the mainboard of the internal computer. See
Figure 1-37 on page 1-49 . It is used to convert analog to digital signals
for Orbitrap analyzer experiments, especially for detecting the ions. The board contains an ADC for the detection of the transient signal, with a frequency range from 10 kHz to 10 MHz. Three anti-aliasing filters for the low, middle and high mass range are automatically selected by the software.
The data acquisition board provides precision timing to control the acquisition. Events with lower timing requirements on accuracy are controlled by the linear ion trap.
Power Supply 2 Board
Thermo Fisher Scientific
Figure 1-39. Data Acquisition Analog board
The diagnostic LEDs listed in Table 1-7 on page 1-51
show the status of the voltages applied to the board. The position of the LEDs on the
board is indicated by a white rectangle in Figure 1-39
.
Table 1-7. Diagnostic LEDs of the Data Acquisition Analog board
Name Color
+5 V Green
-5 V
+3.3 V
Green
Green
Description
+5 V voltage present
-5 V voltage present
+3.3 V voltage present
Normal Operating Condition
On
On
On
The power supply 2 board provides the supply voltages for the data acquisition analog board. It is mounted to the back inside the housing of the internal computer. See
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-51
Functional Description
Printed Circuit Boards
Instrument Control Board
The diagnostic LEDs listed in Table 1-8
show the status of the voltages applied to the board.
Table 1-8. Diagnostic LEDs of the Power Supply 2 board
Name Color
+5.1 V Green
-5.1 V Green
+3.3 V Green
Description
+5.1 V voltage present
-5.1 V voltage present
+3.3 V voltage present
Normal Operating Condition
On
On
On
Figure 1-40 shows the instrument control board. The instrument
control board is located in a housing next to the internal computer. It is connected to the Orbitrap Elite MS main power.
Diagnostic LEDs
Figure 1-40. Instrument Control board
Status LEDs
The instrument control board is used to interface the Velos Pro MS control electronics to the Orbitrap analyzer control electronics. Three signal lines are passed from the Velos Pro MS: a digital, parallel (DAC) bus, a serial SPI bus, and a Link Port Signal line. The instrument control board contains a micro controller, digital and analog converters, and serial port connectors.
1-52
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Power Distribution Board
Thermo Fisher Scientific
Functional Description
Printed Circuit Boards
On the instrument control board, analog signals from vacuum gauges are converted to digital signals and passed to the data system as well as to the power distribution board. (See
.) Turbomolecular pumps
(See
.) are attached to a serial port connector and this is connected via the signal lines to the linear ion trap.
The diagnostic LEDs listed in
Table 1-9 show the status of applied
voltages to the board. The position of the diagnostic LEDs on the board is indicated by a white rectangle in
Table 1-9. Diagnostic LEDs of the Instrument Control board
No.
LD1
LD2
Name
2.5 V
3.3 V
Color Description Normal
Operating Condition
Green 2.55 V Input voltage present On
Green 3.3 V Input voltage present On
LD3 5 V
LD4 -15 V
Green
Green
5 V Input voltage present
-15 V Input voltage present
On
On
LD5 +15 V Green +15 V Input voltage present On
Additionally, the board has four green LEDs that are directly connected to the micro controller. They indicate the state of the micro controller and possible error bits and can be used for software debugging. See
. The position of the status LEDs on the board is indicated
by a white oval in Figure 1-40 on page 1-52
.
Table 1-10. Software status LEDs of the Instrument Control board
No.
Description
6.1
Micro controller is working properly
6.2
CAN bus connection to power distribution board enabled
6.3
Connection to internal computer and Velos
Pro SPI bus enabled
6.4
Orbitrap analyzer SPI bus enabled
Normal Operating Condition
Permanent flashing of LED
On
On
On
Flashing on error
Figure 1-41 on page 1-54 shows the power distribution board. It is
located at the bottom of the right side of the instrument.
The power distribution board controls the vacuum system and the system power supplies, including the linear ion trap. Depending on the quality of the vacuum and the status of the turbo molecular pumps, it switches the vacuum gauges, the pumps, and the 230 V relays. It controls external relays with 24 V DC connections. In case of a vacuum failure, it initiates an automatic power down of the instrument.
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-53
Functional Description
Printed Circuit Boards
Figure 1-41. Power Distribution board
The power distribution board indicates all system states and error
messages by status LEDs (See Table 1-11 on page 1-55
.) in the middle of the left side of the board. A green LED indicates that the status is
OK. An orange LED indicates a status that differs from normal. The position of the LEDs on the board is indicated by a white oval in
.
The system status LEDs on the front side of the instrument (See
.) are controlled by the power distribution board.
The information partially comes from external boards (for example, the
Communication LED is controlled by the instrument control board).
(See “Instrument Control Board” on page 1-52 .)
Diagnostic LEDs show the status of voltages applied from the board to other devices. The positions of the diagnostic LEDs on the board are indicated by white rectangles in
1-54
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Thermo Fisher Scientific
Functional Description
Printed Circuit Boards
Table 1-11. Status LEDs of the Power Distribution board
LED green
Vacuum
Comm.
System
LED orange
High vacuum failure
No communication with instrument control board
System is not ready
Information given by orange LED
High vacuum pressure > 10
-8 mbar
CAN bus problem or instrument control board not working
FT Electronics switch off or
Vacuum Pumps switch off
Scan
Electr. On
Vac. Units OK
Pirani Orbitrap analyzer OK
Pirani LT OK
Ion Gauge On
Ion Gauge OK
Turbo P. 1 On
Rotation 1 OK
Service mode
Vacuum measurement failure
No function, at present
Instrument is not scanning
FT Electronics switch off
Vacuum gauge defective
Pirani Velos Pro MS failure Control signal < 0.5 V
Penning Orbitrap Elite MS
Off
Forevacuum > 10
-2
mbar
Penning Orbitrap Elite MS failure
Control signal < 0.5 V
LT Vacuum Work Velos Pro MS vacuum failure
Vac. <10
-3
Vac. <10
-5
Forevacuum failure
High vacuum failure
Pumps OK
Rough P. 1 On
Pumps Off
Forepump #1 failure
TMP 1 failure
TMP 1 failure
Vacuum forepump Velos Pro MS
>10
-1 mbar
Forevacuum > 10
-3
High vacuum > 10
-5 mbar mbar
Pump down; leakage
Forepump defective
TMP defective/error a
Turbo P. 2 On
Rotation 2 OK
Heater Off
LAN Conn. OK
EI On
A
B
TMP 2 failure
TMP 2 failure
Heater enabled
LAN connection failure
No function, at present
System reset
80% rotation speed of TMP not reached
80% rotation speed of TMP not reached
Heater enabled
LAN interrupted (Option)
System reset has occurred
Micro controller idle a
An error of
TMP
3 is indicated by an LED directly located on the pump controller. An error of TMP 4 is indicated in the software.
Depending on user actions, the power distribution is switched to various
working modes by the hardware. See Table 1-12 on page 1-56
.
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-55
Functional Description
Printed Circuit Boards
Table 1-12. Working modes of the Power Distribution board
Action
a.
Main switch off
Consequences
Complete system including linear ion trap and multiple socket outlets (ETD Module, for example) are without power
Everything is switched off b.
Vacuum Pumps switch off c.
FT Electronics switch off
All components are switched off with exception of the following ones:
• Heater control
• Multiple socket outlets
• Power distribution board
• Pumps
• Vacuum control
• Velos Pro MS (has a separate Service switch)
Table 1-13 shows the possible operating states of the power distribution.
Table 1-13. Operating states of the Power Distribution board
Action
1.
Main switch on, Vacuum Pumps switch off
2.
Vacuum Pumps switch on and FT
Electronics switch on
3.
Check linear ion trap and Orbitrap Elite
MS forevacuum pumps:
10
-0
mbar after 30 s.
4.
After the system has started, the Pirani gauge returns a vacuum < 10
-2 mbar and both TMPs reach 80% rotation speed
5.
Vacuum and 80% rotation speed of
TMPs not reached after preset time
(< 8 min, otherwise the pumps automatically switch off).
6.
One or more vacuum gauges defective
(control signal < 0.5 V).
7.
After the operating status is reached, the pressure at one gauge exceeds the security threshold for more than the preset time period:
• Pirani gauge Orbitrap Elite MS
>10
-1 mbar
• Penning gauge Orbitrap Elite MS
>10
-3 mbar
• Pirani gauge Velos Pro MS forepump
>10
-1 mbar
Consequences
Everything is switched off
System starts up: pumps and electronics switched on
If not ok: switch off system and light error LED a
; power distribution remains switched on
Switch on Penning gauge
Switch off system (including linear ion trap) and light error LED
*
; power distribution remains switched on
Light error LED only, otherwise ignore
System is shut down with exception of power distribution (light error LED).
Rebooting of the system by switching off/on of the main switch.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Power Supply 1 Board
Functional Description
Printed Circuit Boards
Table 1-13. Operating states of the Power Distribution board, continued
8.
Rotation speed of a TMP falls below
9.
10.
11.
12.
Action
80%
Service switch linear ion trap off
FT Electronics switch Orbitrap Elite MS off
Failure of linear ion trap or Orbitrap Elite
MS (for example, fuse is opened).
Mains failure
Consequences
Shut down system (see 7.); light LED
*
of corresponding pump.
Linear ion trap electronics switched off, pumps keep on running;
Orbitrap Elite MS without data link, keeps on running
Orbitrap Elite MS electronics switched off, pumps keep on running;
Orbitrap Elite MS without data link, keeps on running
If the vacuum in one part deteriorates, the complete system is shut down.
System powers up after the electricity is available again. All devices reach the defined state. Linear ion trap and internal computer must reboot.
a
After the shutdown, the LED flashes that represents the reason for the shutdown.
Figure 1-42 shows the power supply 1 board. This board is located next
to the power distribution board. It provides the power for the ion optic supply board (See
“Ion Optic Supply Board” on page 1-59
.) and the
instrument control board. (See “Instrument Control Board” on page 1-52
.)
Thermo Fisher Scientific
Figure 1-42. Power Supply 1 board
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-57
Functional Description
Printed Circuit Boards
Warning
Electrical Shock Hazard. Parts of the power supply 1 board are at high voltage. Shut down the instrument and disconnect it from line power before performing service.
▲
The diagnostic LEDs listed in
Table 1-14 show the status of the voltages
applied to the board. The position of the LEDs on the board is indicated by the white rectangles in
.
Table 1-14. Diagnostic LEDs of the Power Supply 1 board
Name Color Description
+285 V
-285 V
Green
Green
Over Current +285 V Red
+285 V Output voltage present
-285 V Output voltage present
Over Current -285 V Red
LED lit dark red: I out
> 80 mA
LED lit bright red: output is short-circuited
LED lit dark red: I out
> 80 mA
LED lit bright red: output is short-circuited
+18 V
-18 V
+8.5 V
Green
Green
Green
+18 V Output voltage present
-18 V Output voltage present
+8.5 V Output voltage present
On
On
On
Normal Operating
Condition
On
On
Off
Off
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Printed Circuit Boards
Electronic Boards on the Left Side of the Instrument
Figure 1-43 shows the left side of the instrument with the panel opened.
This side of the instrument contains mostly boards that are part of the
Orbitrap analyzer control.
Ion Optic Supply board housing
Central Electrode Pulser board housing
Temperature
Controller board
CLT RF Main board housing with RF Off & Feedback board and CLT Offset Connector
Central Electrode
Power Supply board housing
High Voltage Power Supply board housing
Figure 1-43. Electronic boards on the left side of the instrument
The main components on this side are described starting from the top.
Ion Optic Supply Board
Figure 1-44 on page 1-60 shows the ion optic supply board. The board
is located in a housing on top of the left instrument side of the instrument. This board supplies the voltages and the radio frequency for the ion guides and interoctapole lenses of the Orbitrap Elite mass spectrometer. It has an RF detector for the RF output control. The
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-59
Functional Description
Printed Circuit Boards board also provides the trap voltage, the gate voltage, and the reflector
DC voltages as well as the RF voltages to the octapole of the Orbitrap
analyzer. See “Orbitrap Analyzer” on page 1-14 for further information.
Figure 1-44. Ion Optic Supply board
The diagnostic LEDs listed in Table 1-15
show the status of applied voltages to the board. The position of the LEDs on the board is indicated by white rectangles in
Warning
Electrical Shock Hazard. Parts of the board are at high voltage. Shut down the instrument and disconnect it from line power before performing service.
▲
Table 1-15. Diagnostic LEDs of the Ion Optic Supply board
No.
Name Color Description
LD1 +275 V Green +275 V Input voltage present
LD2 -275 V Green -275 V Input voltage present
LD3 +29 V
LD5 +15 V
LD6 -15 V
Green
Green
Green
+29 V Input voltage present
+15 V Input voltage present
-15 V Input voltage present
On
On
On
Normal Operating
Condition
On
On
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Printed Circuit Boards
Table 1-15. Diagnostic LEDs of the Ion Optic Supply board, continued
No.
Name Color Description
LD7 RF1_ON Blue RF1 generator switched on
LD8 RF2_ON Blue RF2 generator switched on
Normal Operating
Condition
depending on application;
LED flashes during scanning depending on application;
LED flashes during scanning
Central Electrode Pulser Board
The central electrode pulser board is located in a housing that is
mounted to the flange of the UHV chamber. See Figure 1-45
.
Thermo Fisher Scientific
Figure 1-45. Central Electrode Pulser board
The board switches the injection and measurement voltages for the central electrode and the detection electrodes of the Orbitrap analyzer.
Resistor-capacitor circuits on the board convert the switching pulse into a smooth transition between the voltages.
The diagnostic LEDs listed in
Table 1-16 on page 1-62 show the status
of the voltages applied to the board as well as some operating states. The position of the LEDs on the board is indicated by the white rectangles in
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-61
Functional Description
Printed Circuit Boards
Table 1-16. Diagnostic LEDs of the Central Electrode Pulser board
No.
Name Color Description
LD1 TRIG Green Trigger signal indicator
LD2 PS
Normal Operating Condition
Flashing when scanning
Green 24V Power Supply is OK On
Temperature Controller Board
The temperature controller board is located on the top left side of the instrument, next to the CLT RF main board. See
Figure 1-43 on page 1-59 . The temperature controller board keeps the temperature of
the analyzer chamber to a preset value. A Peltier element that can be used for heating as well as for cooling is used as an actuator. Activation is done via the serial SPI (Serial Peripheral Interface) bus.
Figure 1-46. Temperature Controller board
The diagnostic LEDs listed in Table 1-17 on page 1-63
show the status of the voltages applied to the board as well as some operating states. The positions of the LEDs on the board are indicated by the white rectangles in
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
CLT RF Unit
Functional Description
Printed Circuit Boards
Table 1-17. Diagnostic LEDs of the Temperature Controller board
No.
Name Color Description
LD1 +15 V
LD2 -15 V
Green +15 V Input voltage present
Green -15 V Input voltage present
LD3
LD4
TEC >60C
Unit >60C
Yellow Temperature of cold side Peltier element above 60 ºC
Yellow Temperature of UNIT heat sink above 60 ºC
Yellow Control switched off LD5 Reg Off
LD6 No Term Yellow SPI bus termination board missing
LD7 SDT enable Green Interface has been addressed and sends/receives data
LD8 SEL Green Board has been addressed
LD9 Heating
LD10 Cooling
LD11 UR>0
Yellow Peltier element is heating
Yellow Peltier element is cooling
Normal Operating
Condition
On
On
Off
Off
Off
Off
Flashing on SPI bus data transfer
Flashing on SPI bus data transfer
Depending on system state
Depending on system state
Off when adjusted
LD12 UR<0
Yellow Summation voltage controller
>0 V
Yellow Summation voltage controller
<0 V
Off when adjusted
The CLT RF unit comprises the CLT RF main board and the RF off & feedback board. The unit operates the curved linear trap (CLT) with four phases RF voltage and three pulsed DC voltages (PUSH, PULL, and OFFSET).
The CLT RF main board is located in a housing in the center of the left side of the instrument. See
Figure 1-43 on page 1-59 . This board
provides an RF voltage (“Main RF”) for the curved linear trap. It allows switching off the RF and simultaneous pulsing of each CLT electrode.
See
“Orbitrap Analyzer” on page 1-14
for further information. The board communicates with the instrument control board via an SPI bus.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-63
Functional Description
Printed Circuit Boards
CLT Offset Connector RF Off & Feedback board
Figure 1-47. CLT RF unit (cover removed)
The diagnostic LEDs listed in
Table 1-18 show the status of the voltages
applied to the board as well as some operating states. The position of the
LEDs on the board is indicated by the white rectangles in Figure 1-47
.
Table 1-18. Diagnostic LEDs of the CLT RF Main board
No.
Name Color Description
LD1 NO TERM Yellow SPI bus termination board missing
LD2 SEND Yellow Interface has been addressed and sends/receives data
LD3 SEL Green Board has been addressed
LD4 RF ON
LD6 OVL
LD7 OVHEAT
Green RF voltage on
LD5 NO LOCK Yellow PLL has been not locked
Yellow
Red
RF Amplifier overload
Heatsink temperature > 73 °C
Normal Operating
Condition
Off
Flashing on SPI bus data transfer
Flashing on SPI bus data transfer
On
50% intensity
Off
Off
The RF off & feedback board is an add-on board to the CLT RF main board. It is located in the same housing. See
The CLT Offset connector, which removes interfering signals from the circuit, is also mounted in the housing.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Printed Circuit Boards
Central Electrode Power Supply Board
The central electrode power supply board is mounted in a housing on
the bottom left side of the instrument. See Figure 1-48
.
Figure 1-48. Central Electrode Power Supply board
The board supplies four DC voltages to the Orbitrap analyzer:
•
Two central electrode (CE) voltages: CE HIGH and CE LOW.
•
Two deflector electrode (DE) voltages: DE HIGH and DE LOW.
For positive ions, the CE voltages are negative and the DE voltages are positive. The maximum CE voltage is 3 kV and the maximum
DE voltage is 1 kV. The board communicates via the SPI bus.
In addition to a ventilator on the bottom right side, a water-cooled
Peltier element on the rear side of the board serves as means of heat dissipation.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-65
Functional Description
Printed Circuit Boards
The diagnostic LEDs listed in
Table 1-19 show the status of the voltages
applied to the board as well as some operating states. The position of the
LEDs on the board is indicated by the red rectangles in
Table 1-19. Diagnostic LEDs of the Central Electrode Power Supply board
No.
Name Color Description
LD1 OVL DE HIYellow Negative side of Deflector High
Supply has been overloaded
LD2 OVL DE HI+ Yellow Positive side of Deflector High
Supply has been overloaded
LD3 No Term Red SPI bus termination board missing
LD4 Send
LD5 Sel
Yellow Interface has been addressed and sends/receives data
Green Board has been addressed
Normal Operating
Condition
Off when HV is switched on
Off when HV is switched on
Off
Flashing on SPI bus data transfer
Flashing on SPI bus data transfer
Off (positive mode)
Off when HV is switched on
LD6 Polarity Blue Positive/negative ion mode
LD7 OVL CE LO+ Yellow Positive side of Central
Electrode Low Supply has been overloaded
LD8 OVL CE LOYellow Negative side of Central
Electrode Low Supply has been overloaded
LD9 OVL CE HI+ Yellow Positive side of Central
Electrode High Supply has been overloaded
LD10 OVL CE HIYellow Negative side of Central
Electrode High Supply has been overloaded
LD11 OVL DE LO+ Yellow Positive side of Deflector Low
Supply has been overloaded
LD12 OVL DE LOYellow Negative side of Deflector Low
Supply has been overloaded
LD13 HV ON Green High voltage switched on
Off when HV is switched on
Off when HV is switched on
Off when HV is switched on
Off when HV is switched on
Off when HV is switched on
On when HV is switched on
High Voltage Power Supply Board
The high voltage power supply board is mounted in a housing on the
bottom left side of the instrument. See Figure 1-43 on page 1-59
. This board provides five DC voltages for the ion optics of the Orbitrap Elite mass spectrometer. Two voltages supply the lenses of the instrument.
Three voltages are applied to the RF CLT main board to be used as
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Functional Description
Printed Circuit Boards focusing potentials for the curved linear trap. See
“Orbitrap Analyzer” on page 1-14 for further information. The board communicates via the
SPI bus.
Warning
Electrical Shock Hazard. The high voltage power supply board creates voltages up to 3.5 kV! Shut down the instrument and disconnect it from line power before performing service.
▲
Figure 1-49. High Voltage Power Supply board (cover removed)
The diagnostic LEDs listed in
Table 1-20 on page 1-68 show the
operating states of the board. The position of the LEDs on the board is
indicated by the white rectangles in Figure 1-49 .
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
1-67
Functional Description
Printed Circuit Boards
SPI Bus Termination Board
Table 1-20. Diagnostic LEDs of the High Voltage Power Supply board
No.
Name Color Description Normal Operating
Condition
Off LD1
LD2
LD3
NO TERM
SEND
SEL
Red SPI bus termination board missing
Yellow Interface has been addressed and sends/receives data
Green Board has been addressed
LD4 HV ON Green High voltage is switched on
LD5 POLARITY Green Positive/negative ion mode
Flashing on SPI bus data transfer
Flashing on SPI bus data transfer
On
Off (positive mode)
Various boards communicate via the SPI bus, a serial RS485-based bus system. The SPI Bus Termination board reduces unwanted signal reflections. The boards indicate a missing termination (after maintenance, for example) by LEDs.
The SPI Bus Termination board is located below the High Voltage
Power Supply board, at the bottom left side of the instrument. See
.
SPI bus termination board
Figure 1-50. High Voltage Power Supply board with SPI Bus Termination board
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Chapter 2
Basic System Operations
Many maintenance procedures for the Orbitrap Elite system require that the mass spectrometer be shut down. In addition, the Orbitrap Elite system can be placed in Standby condition if the system is not to be used for 12 hours or more.
The following topics are described in this chapter:
•
“Shutting Down the System in an Emergency” on page 2-2
•
“Placing the Instrument in Standby Condition” on page 2-4
•
“Shutting Down the Orbitrap Elite Mass Spectrometer Completely” on page 2-7
•
“Starting Up the System after a Shutdown” on page 2-9
•
“Resetting the System” on page 2-12
•
“Resetting Tune and Calibration Parameters to their Default Values” on page 2-13
•
“Turning Off the Reagent Ion Source: What to Expect” on page 2-14
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
2-1
Basic System Operations
Shutting Down the System in an Emergency
Shutting Down the System in an Emergency
If you need to turn off the mass spectrometer in an emergency, place the main power switch (located on the power panel at the right side of the
Orbitrap Elite mass spectrometer) in the Off (0) position. This turns off all power to the instrument, including the linear ion trap, multiple socket outlets, and the vacuum pumps. The main power switch must be turned 90° anti-clockwise to switch off the instrument. See
On
Off
Figure 2-1. Main power switch in Off position
The instrument is automatically vented by the vent valve of the linear ion trap. The vent valve vents the system 30 seconds after power is switched off.
Although removing power abruptly will not harm any component within the system, this is not the recommended shutdown procedure to
follow. See “Shutting Down the Instrument” on page 2-7 for the
recommended procedure.
Note To separately turn off the recirculating chiller or computer in an emergency, use the On/Off switches on the chiller and computer, respectively.
▲
Behavior of the System in Case of a Main Failure
A main power failure has the same consequence as switching off via the main power switch. If the power is available again, the system starts up automatically: the pumps are switched on and the instrument is pumped down. If the system has been vented during the mains failure, it is necessary to bake out the system to obtain the operating vacuum. See
“Baking Out the System” on page 3-4
.
It is not possible to check whether the system was vented. The log file of the data system indicates a reboot of the system. In case of frequent but short power failures we recommend installing an uninterruptible power
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Basic System Operations
Shutting Down the System in an Emergency supply (UPS). If main power failures occur frequently while the system is not attended (for example, in the night), we recommend installing a power fail detector.
Note The intentional venting of the system is performed with the vent valve of the linear ion trap.
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2-3
Basic System Operations
Placing the Instrument in Standby Condition
Placing the Instrument in Standby Condition
The Orbitrap Elite system should not be shut down completely if you are not going to use it for a short period of time, such as overnight or over the weekend. When you are not going to operate the system for
12 hours or more, you can leave the system in Standby condition.
In case of an Orbitrap Elite ETD mass spectrometer, first place the
ETD Module in Standby condition. Then place the mass spectrometer in Standby condition according to the procedures described in the following topics.
Placing the ETD Module in Standby Condition
❖
To place the ETD Module in Standby condition
1. If the Tune Plus window is not already open, choose Start >
Programs > Thermo Instruments > LTQ > LTQ Tune from the taskbar. The Tune Plus window will open.
On/Standby button Reagent Ion Source instrument control icon
Figure 2-2. Tune Plus window (Orbitrap Elite ETD), toolbar
On Off Standby
You can determine the state of the mass spectrometer by observing the state of the On/Off/Standby button on the Control/Scan Mode toolbar. See
Figure 2-2 . The three different states of the On/Standby
button are shown at the left.
2. Click the Reagent Ion Source portion of the instrument control
graphic at the top of the Tune Plus window. (See Figure 2-2
.) The
Reagent Ion Source dialog box appears. (See Figure 2-3 .)
3. In the Reagent Ion Source dialog box, clear the Reagent Ion Source
On box to place the Reagent Ion Source in Standby condition. See
. This places the Reagent Ion Source in
Standby condition as indicated by the Actual condition shown to the right of the Reagent Ion Source On box.
When the reagent ion source is placed in Standby condition, the filament and vial heaters turn off. Simultaneously, a valve opens to allow the nitrogen gas to cool the reagent vials. This cooling
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Basic System Operations
Placing the Instrument in Standby Condition nitrogen runs until the reagent vials reach 70 °C. The audible rush
(hissing noise) of nitrogen from the reagent ion source area in the back of the ETD Module is normal operation.
Thermo Fisher Scientific
Figure 2-3. Reagent Ion Source dialog box with Reagent Ion Source On box and Actual condition circled
If the reagent ion source is on when you place the Orbitrap
Elite ETD mass spectrometer in Standby mode, the filament turns off immediately. In contrast, the vial heaters stay on for 60 minutes before they turn off and the cooling gas begins. Because the filament is turned off, you can perform minor maintenance procedures on the ETD Module without cooling the reagent inlet.
Warning
Burn Hazard. Install or exchange the reagent vials by
following the procedure in “Replacing the Reagent Vials” on page 3-48.
The reagent vials will be too hot to touch after the cooling nitrogen turns off at 70°C. Verify that the reagent vials are cool to the touch before handling them.
▲
More information about turning on and off the reagent heaters is
given in “Reagent Heaters” on page 1-27 .
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
2-5
Basic System Operations
Placing the Instrument in Standby Condition
Warning
Burn Hazard. The restrictor, the transfer line, and the ion source heater operate at 160 °C. Do not attempt to touch them unless
these heaters have had sufficient time to cool down to room temperature.
▲
Placing the MS in Standby Condition
❖
To place the Orbitrap Elite system in Standby condition
1. Wait until data acquisition, if any, is complete.
2. Turn off the flow of solvent from the LC (or other sample introduction device).
Note For instructions on how to operate the LC from the front panel, refer to the manual that came with the LC.
▲
On Off Standby
3. From the Tune Plus window, choose Control > Standby (or click on the On/Standby button to toggle it to Standby) to put the instrument in Standby condition. The consequences of this user action are described in the LTQ Series Hardware Manual. The
System LED on the front panel of the Velos Pro mass spectrometer turns yellow when the system is in Standby condition.
4. Leave the LC power on.
5. Leave the autosampler power on.
6. Leave the data system power on.
7. Leave the Orbitrap Elite MS main power switch in the On position.
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Basic System Operations
Shutting Down the Orbitrap Elite Mass Spectrometer Completely
Shutting Down the Orbitrap Elite Mass Spectrometer Completely
The Orbitrap Elite mass spectrometer does not need to be shut down completely if you are not going to use it for a short period of time, such as overnight or over weekends. Shut down ETD Module and MS system completely only if you do not want to use them for an extended period or if you want to perform a maintenance or service procedure.
❖
To shut down the instrument completely
1. Place the ETD Module in Standby condition as described in
“Placing the ETD Module in Standby Condition” on page 2-4 .
2. Shut down the instrument as described in
Instrument“ below. This also shuts down the ETD Module because
its power controls are linked to the Orbitrap Elite MS power controls through the ETD Module Interface board. See
“ETD Module Interface Board” on page 1-25 .
Shutting Down the Instrument
❖
To shut down the Orbitrap Elite system
1. Wait until data acquisition, if any, is complete.
2. Turn off the flow of solvent from the LC (or other sample introduction device).
Note For instructions on how to operate the LC from the front panel, refer to the manual that came with the LC.
▲
3. From the Tune Plus window, choose Control > Off to put the instrument in Off condition. When you choose Control > Off, all high voltages are shut off, as are the flows of the sheath gas and the auxiliary gas.
4. Put the FT Electronics switch to the Off position. See Figure 1-7 on page 1-8
.
5. Put the Vacuum Pumps switch to the Off position. See
When you place the switch in the Off position, the following occurs: a. All power to the instrument, including the turbomolecular pumps and the rotary-vane pumps, is turned off.
b. After 30 s, power to the vent valve solenoid of the ion trap is shut off. The vent valve opens and the vacuum manifold is vented with nitrogen to atmospheric pressure through a filter.
You can hear a hissing sound as the gas passes through the filter.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
2-7
Basic System Operations
Shutting Down the Orbitrap Elite Mass Spectrometer Completely
6. Leave the main power switch of the Orbitrap Elite mass spectrometer in the On position.
7. During service or maintenance operations that require opening the vacuum system of the Velos Pro MS or the Orbitrap Elite MS, always put the main switch (main circuit breaker) to the Off position. You can secure the main switch with a padlock or tie-wrap to prevent unintended re-powering.
Warning
Burn Hazard. Allow heated components to cool down before you service them (the ion transfer tube is operated at about 300 °C, for example).
▲
Note If you are planning to perform routine or preventive system maintenance on the Orbitrap Elite mass spectrometer only, you do not need to turn off the recirculating chiller, LC, autosampler, or data system. In this case, the shutdown procedure is completed. However, if you do not plan to operate your system for an extended period of time, you might want to turn off the recirculating chiller, LC, autosampler, and data system.
▲
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Basic System Operations
Starting Up the System after a Shutdown
Starting Up the System after a Shutdown
To start up the Orbitrap Elite mass spectrometer after it has been shut down, you need to do the following:
1. Start up the instrument.
2. Set up conditions for operation.
Starting Up the Instrument
Note The recirculating chiller and data system must be running before you start up the instrument. The instrument will not operate until it has established a communication link to the data system.
▲
❖
To start up the Orbitrap Elite mass spectrometer
1. Start up the (optional) LC and autosampler as is described in the manual that came with the LC and autosampler.
2. Start up the data system and the chiller.
3. Turn on the flows of helium and nitrogen at the tanks, if they are off.
4. Make sure that the main power switch of the Velos Pro MS is in the
On position and the electronics service switch of the Velos Pro MS is in the Operating position.
5. Place the main power switch at the right side of the Orbitrap Elite mass spectrometer in the On position.
6. Put the Vacuum Pumps switch to the On position. See
. The rotary-vane pumps and the turbomolecular pumps are started.
Note Pumping the system after a complete shut down takes hours and requires overnight baking of the system.
▲
7. Put the FT Electronics switch to the On position. See Figure 1-7
.
When you place the FT Electronics switch to the On position, the following occurs: a. Power is provided to all electronic boards. (The electron multiplier, conversion dynode, 8 kV power to the API source, main RF voltage, and quadrupole RF voltage remain off.) b. The internal computer reboots. After several seconds, the
Communication LED on the front panel turns yellow to
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
2-9
Basic System Operations
Starting Up the System after a Shutdown indicate that the data system has started to establish a communication link.
c. After several more seconds, the Communication LED turns green to indicate that the data system has established a communication link. Software for the operation of the instrument is then transferred from the data system to the instrument.
d. After three minutes, the System LED of the ion trap turns yellow to indicate that the software transfer from the data system is complete and that the instrument is in Standby condition.
Note The Vacuum LED on the front panel of the Velos Pro turns green only if the pressure in the vacuum manifold is below the maximum allowable pressure (5×10
-4
Torr in the analyzer region, and 2 Torr in the
S-lens region), and the safety interlock switch on the API source is pressed down (that is, the API flange is secured to the spray shield).
▲
8. Press the Reset button on the Velos Pro to establish the communication link between Velos Pro and internal computer.
If you have an LC or autosampler, start it as is described in the manual
that came with the LC or autosampler. Then, proceed to “Setting Up
Conditions for Operation“ . If you do not have either, go to the topic
directly.
Setting Up Conditions for Operation
❖
To set up your Orbitrap Elite mass spectrometer for operation
1. Before you begin data acquisition with your Orbitrap Elite system, you need to allow the system to pump down for at least eight hours.
Operation of the system with excessive air and water in the vacuum manifold can cause reduced sensitivity, tuning problems, and a reduced lifetime of the electron multiplier.
Note The vacuum in the analyzer system can be improved by an overnight baking of the system. See
“Baking Out the System” on page 3-4 .
▲
2. Ensure that the gas pressures are within the operational limits:
•
Helium: 275 ± 70 kPa (2.75 ± 0.7 bar, 40 ±10 psi),
•
Nitrogen: 690 ± 140 kPa (6.9 ± 1.4 bar, 100 ± 20 psi).
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Basic System Operations
Starting Up the System after a Shutdown
In case of an Orbitrap Elite ETD mass spectrometer, also check the pressure of the reagent carrier gas: 690 ± 140 kPa (6.9 ± 1.4 bar,
100 ± 20 psi).
Note Air in the helium line must be purged or given sufficient time to be purged for normal performance.
▲
3. Click the Display Status View button in the Tune Plus window.
Check whether the pressure measured by the ion gauge is
≤
5 × 10
-9 mbar, and the pressure measured by the Pirani gauge is around 1 mbar. Compare the values of the other parameters in the status panel with values that you recorded previously.
4. In case of an Orbitrap Elite ETD mass spectrometer, start up the
ETD Module as described in “Starting the ETD Module After a
Complete Shutdown“ . In case of an Orbitrap Elite mass
spectrometer, continue to set up for ESI or APCI operation as described in Orbitrap Elite Getting Started.
Starting the ETD Module After a Complete Shutdown
On Off Standby
❖
To start up the ETD Module after a complete shutdown
1. Start the Orbitrap Elite ETD mass spectrometer according to the start up procedures given in
“Starting Up the System after a
above. This also turns on the ETD Module as the
ETD Module power controls are linked to the MS power controls
(see
“ETD Module Interface Board” on page 1-25
).
2. If the Tune Plus window is not already open, choose Start >
Programs > Thermo Instruments > LTQ > LTQ Tune from the taskbar. The Tune plus window will open.
You can determine the state of the MS detector by observing the state of the On/Off/Standby button on the Control/Scan Mode
toolbar. (See Figure 2-2 on page 2-4
.) The three different states of the On/Standby button are shown at the left.
3. Click the Display Status View button in the Tune Plus window.
Check the reagent vacuum parameters:
•
Reagent ion gauge pressure: 20 to 35 × 10
-5
Torr
•
Reagent Convectron gauge pressure: <0.08 Torr
•
Reagent turbomolecular pump speed : > 90%
4. Continue to set up the instrument for operation as described in
Orbitrap Elite Getting Started manual.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
2-11
Basic System Operations
Resetting the System
Resetting the System
If the communication link between Orbitrap Elite mass spectrometer and data system computer is lost, it may be necessary to reset the system using the Reset button of the Velos Pro mass spectrometer.
The procedure given here assumes that the Orbitrap Elite mass spectrometer and data system computer are both powered on and are operational. If the instrument, data system computer, or both are off, see
“Starting Up the System after a Shutdown” on page 2-9
.
To reset the Orbitrap Elite mass spectrometer, press the Reset button of the Velos Pro. See the LTQ Series Hardware Manual for the location of the Reset button. When you press the Reset button, the following occurs:
1. An interrupt on the mainboard of the internal computer causes the internal computer to reboot. All LEDs on the front panel are off except the Power LED.
2. After several seconds, the Communication LED turns yellow to indicate that the data system and the instrument are starting to establish a communication link.
3. After several more seconds, the Communication LED turns green to indicate that the data system and the instrument have established a communication link. Software for the operation of the instrument is then transferred from the data system to the instrument.
4. After three min, the software transfer is complete. The System LED turns either green to indicate that the instrument is functional and the high voltages are on or yellow to indicate that the instrument is functional and it is in Standby condition.
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Basic System Operations
Resetting Tune and Calibration Parameters to their Default Values
Resetting Tune and Calibration Parameters to their Default Values
You can reset the Orbitrap Elite system tune and calibration parameters to their default values at any time. This feature may be useful if you have manually set some parameters that have resulted in less than optimum performance.
❖
To reset the Orbitrap Elite MS tune and calibration parameters
In the Tune Plus window,
•
Choose File > Restore Factory Calibration to restore the default calibration parameters, or
•
Choose File > Restore Factory Tune Method to restore the default tune parameters.
Note Make sure that any problems you might be experiencing are not due to improper API source settings (spray voltage, sheath and auxiliary gas flow, ion transfer capillary temperature, etc.) before resetting the system parameters to their default values.
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Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
2-13
Basic System Operations
Turning Off the Reagent Ion Source: What to Expect
Turning Off the Reagent Ion Source: What to Expect
In the Orbitrap Elite ETD mass spectrometer, the reagent ion source
controls can be accessed as described in “Placing the ETD Module in
Standby Condition” on page 2-4 . When you deselect the Reagent Ion
Source On check box in the Reagent Ion Source dialog box (See
.), the ETD source and reagent heaters are placed in Standby condition.
Figure 2-4. Placing the reagent ion source in Standby condition
When the ETD Module is placed in Standby condition, the filament and vial heaters are turned off. Simultaneously a valve opens to allow nitrogen gas to cool the reagent vials. This cooling nitrogen runs until the vials reach 70 °C. The audible rush (hissing noise) of nitrogen from the reagent ion source area in the back of the ETD Module is normal operation.
Warning
Burn Hazard. The reagent vials are too hot to handle after the cooling nitrogen turns off at a vial temperature of 70 °C. Verify that the reagent vials have cooled down to a safe temperature before handling them. This can take up to 90 minutes after the cooling nitrogen has turned off.
▲
Other conditions that will cause the ETD Module to remain in
Standby:
•
Attempting to turn on the reagent ion source when the restrictor heater, transfer line heater, and the source heater are not at their target temperatures.
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Basic System Operations
Turning Off the Reagent Ion Source: What to Expect
•
Whenever either the mass spectrometer or the ETD Module goes into Standby mode. Reagent vial nitrogen cooling will turn on if the vials are at an elevated temperature.
Exception: If the Orbitrap Elite ETD mass spectrometer is placed in
Standby by clicking the Standby button in Tune Plus (see Standby icon in the margin), there is an hour delay before the cooling nitrogen turns on.
▲
•
Whenever the pressure in the mass spectrometer or the
ETD Module exceeds its protection limit. Reagent vial nitrogen cooling will turn on if the vials are at an elevated temperature.
•
Whenever the abundance of reagent ions becomes insufficient as determined by the AGC setting. When this occurs, the Orbitrap
Elite ETD mass spectrometer completes the Xcalibur Sequence step in progress before going into Standby mode.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
2-15
Chapter 3
User Maintenance
This chapter describes routine maintenance procedures that must be performed to ensure optimum performance of the Orbitrap Elite mass spectrometer.
For instructions on maintaining the Velos Pro linear trap, refer to the
LTQ Series Hardware Manual. For instructions on maintaining LCs or autosamplers, refer to the manual that comes with the LC or autosampler.
Note It is the user’s responsibility to maintain the system properly by performing the system maintenance procedures on a regular basis.
▲
The following topics are described in this chapter:
•
•
“Baking Out the System” on page 3-4
•
“Maintenance of the Vacuum System” on page 3-4
•
“Maintenance of the ETD Module” on page 3-12
•
“Maintenance of the Cooling Circuit” on page 3-58
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
3-1
User Maintenance
General Remarks
General Remarks
Preventive maintenance must commence with installation, and must continue during the warranty period to maintain the warranty. Thermo
Fisher Scientific offers maintenance and service contracts. Contact your local Thermo Fisher Scientific office for more information. Routine and infrequent maintenance procedures are listed in
Table 3-1. User maintenance procedures
MS Component
Analyzer
Rotary-vane pumps
Turbomolecular pumps
Cooling water circuit
ETD Module
Procedure
System bakeout
Add oil
Change oil
Replace operating fluid reservoir and pump bearings
Change rotary-vane pump oil
Clean rear cooling fans
Frequency
If necessary (for example, after performing maintenance work on the vacuum system)
If oil level is low
Every three months or if oil is cloudy or discolored
Every four years
Check cooling fluid level
Check cooling fluid filter
Check air inlet filter
See manufacturer’s documentation
Replace filter cartridge
Clean ion volume
Annually
As needed
Replace inlet valve components
a
Clean ion source lenses
Clean ion source
Replace ion source filament
Replace reagent vials
Check rotary-vane pump oil and add when needed
Every month
Every four months
Every four months
Procedure Location
Manufacturer’s documentation
Manufacturer’s documentation
Manufacturer’s documentation
Manufacturer’s documentation
page 3-8
a
As needed depends on how close the component is to the electron transfer reagent introduction point. For example, the ion volume is closer to the fluoranthene introduction point than any other component and requires the most frequent cleaning.
To successfully carry out the procedures listed in this chapter, observe the following rules:
•
Proceed methodically.
•
Always wear clean, lint-free, and powder-free gloves when handling the components of the API source, ion optics, mass analyzer, and ion detection system.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
User Maintenance
General Remarks
Thermo Fisher Scientific recommends the following gloves: white nitrile clean room gloves (Fisher Scientific P/N 19-120-2947B [size medium] or P/N 19-120-2947C [size large]; Thermo Scientific
P/N 23827-0008) [size medium] or P/N 23827-0009 [size large]).
•
Never re-use gloves after you remove them because the surface contaminants on them will re-contaminate clean parts.
•
Always wear protective eye wear when you clean parts.
•
Always place the components on a clean, lint-free, and powder-free surface.
•
Always cover the opening in the top of the vacuum manifold with a large, lint-free tissue whenever you remove the top cover plate of the vacuum manifold.
•
Never overtighten a screw or use excessive force.
•
Dirty tools can contaminate your system. Keep the tools clean and use them exclusively for maintenance and service work at the
Orbitrap Elite mass spectrometer.
•
Never insert a test probe (for example, an oscilloscope probe) into the sockets of female cable connectors on PCBs.
Returning Parts
To protect our employees, we ask you for some special precautions when returning parts for exchange or repair to the factory. Your signature on the Repair Covering letter confirms that the returned parts have been de-contaminated and are free of hazardous materials. See
”Safety Advice for Possible Contamination” on page viii
for further information.
Cleaning the Surface of the Instrument
Clean the outside of the instrument with a dry cloth. For removing stains or fingerprints on the surface of the instrument (panels, for example), slightly dampen the cloth (preferably made of microfiber) with distilled water.
Caution
Prevent any liquids from entering the inside of the instrument.
▲
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User Maintenance
Maintenance of the Vacuum System
Maintenance of the Vacuum System
This section contains instructions for performing a system bakeout and for performing pumps maintenance.
Baking Out the System
Collected or remaining gases and molecules as well as moisture can lead to an increased number of collisions with sample ions in the high vacuum region of the instrument. The bakeout procedure removes these contaminations. Therefore, we recommend to bake out the instrument if the high vacuum decreases noticeably during routine operation.
Bakeout is mandatory after maintenance or service work is performed in the analyzer region where the system is vented.
Note Pumping down the system after venting takes at least eight hours, and usually requires overnight baking of the system.
▲
In case the system has been vented during a power failure, it is necessary to bake out the system to obtain the operating vacuum. See
“Behavior of the System in Case of a Main Failure” on page 2-2 .
Bakeout Procedure
❖
To perform a system bakeout
1. Place the system in Standby condition as described in
Instrument in Standby Condition” on page 2-4
.
2. Put the FT Electronics switch at the power control panel into the
On position.
Elapsed time display
Set time display
Up keys
Down keys
Bakeout start button Bakeout stop button
Figure 3-1. Bakeout timer
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3. Set the bakeout time by entering the desired time (hh:mm) with the up/down keys of the bakeout timer. See
4. Start the bakeout procedure by pressing the green start button on the right. The Orbitrap Elite mass spectrometer indicates a running bakeout procedure by the flashing Vacuum and System LEDs on the
front side of the instrument. See Figure 1-4 on page 1-5
.
You can stop a running bakeout procedure by pressing the orange reset/stop button on the left side. Also press this button after you have changed the preset bakeout time.
5. The bakeout procedure is terminated because of two reasons:
•
The preset duration has expired, or
•
The vacuum has risen above a preset value.
The termination of the baking process is indicated by the status LEDs
(System and Vacuum) on the front side that have stopped flashing.
Maintenance of the Forepumps
Rotary-vane pumps require minimal maintenance. All that is required to maintain the rotary-vane pump is to inspect, add, purge, and change the pump oil.
For maintenance of the forepumps of the MS portion, refer to the
LTQ Series Hardware Manual or the pump manufacturer’s manual.
Note The manuals of the pump manufacturers give detailed advice regarding safety, operation, maintenance, and installation. Please note the warnings and precautions contained in these manuals!
▲
Maintenance of the ETD Forepump
Rotary-vane pump oil (P/N A0301-15101) is a translucent light amber color and it should be checked often. During normal operation, oil must always be visible in the oil level sight glass between the MIN and
MAX marks. If the oil level is below the MIN mark, add oil. If the oil is cloudy or discolored, purge the oil to decontaminate dissolved solvents.
If the pump oil is still discolored, change it. You should change the pump oil every 3000 hours (about four months) of operation.
The rotary-vane pump major components are shown in Figure 3-2 on page 3-6
.
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1
2
3
3
14
4
5
6
7
13
8
12
11
9
10
Labeled components: 1=Foreline Vacuum Hose, 2=Electrical Inlet Connector,
3=Voltage Indicator, 4=Inlet Port, 5=Gas Ballast Control, 6=Oil Filler Plugs,
7=Outlet Port, 8=MAX Marks, 9=Oil Drain Plug, 10=MIN Marks, 11=Oil Level
Sight Glass, 12=Mode Selector, 13= On/Off Switch, 14=Lifting Handle
Figure 3-2. Schematic of ETD forepump
Note During normal operation, the mode selector switch is set to high-vacuum mode (turned fully clockwise) and the gas-ballast control is closed (0).
▲
Accessing the ETD Forepump
As described in
“Forepump of the ETD Module” on page 1-34 , the
ETD forepump is located in a cabinet at the bottom of the
ETD Module. To access the ETD forepump, you have to remove the lower panel as indicated in
.
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Pull to remove panel
Loosen screws on both sides
Figure 3-3. Accessing the ETD Forepump: Removing the panel
Two pairs of hooks under the top panel hold the bottom panel. They mount into corresponding openings at the top side of the bottom panel.
Figure 3-4 shows the details for the right side of the instrument. The
panel hangs on the hooks and comes off if lifted up a little and getting pulled on into the backwards direction.
Figure 3-4. Hooks (left) and top side of detached bottom panel (right)
On the bottom of the rear side of the MS portion, two Allen screws fix the panel to the instrument frame by means of fork-like extensions
(lugs). See
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Figure 3-5. Lugs for fixing the bottom panel
❖
To remove the panel
1. Use a 6 mm Allen wrench to loosen the screws that fix the bottom panel. Take care not to loosen the screws completely.
2. Pull the panel horizontally away from the instrument until it comes clear from the hooks.
3. Remove the panel from the instrument and store it at a safe place.
To reattach the panel, proceed in the reverse order.
Adding Oil to the ETD Forepump
The pump oil level must be between the MIN and MAX marks on the oil level sight glass for the pump to operate properly. Pump oil
(P/N A0301-15101) is added as needed when the oil level is below the
MIN mark on the oil level sight glass.
You can check the oil level by looking at the oil level sight glass, which is shown in
Figure 3-2 on page 3-6 . If the ETD forepump oil level is low,
follow these steps to add more oil.
❖
To add oil to the ETD forepump
1. Shut down and vent the Orbitrap Elite ETD mass spectrometer.
Caution
Shut down and unplug the instrument before adding oil.
▲
2. Remove the lower panel at the rear side of the ETD Module as
.
3. Remove one of the oil filler plugs from the rotary-vane pump.
Caution
To maintain optimal performance and prevent damage to the
ETD forepump, only use factory-approved rotary-vane pump oil.
▲
4. Add fresh oil to the reservoir until the oil is half way between the
MIN and MAX level marks. If the oil level goes above the
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MAX level mark, remove the drain plug and drain the excess oil into a suitable container.
5. Insert the oil filler plug back into the rotary-vane pump.
6. Reattach the lower panel at the rear side of the ETD Module.
7. Plug in the instrument.
8. Restart the system.
Purging the Rotary-Vane Pump Oil
When the rotary-vane pump oil becomes cloudy or discolored, purge the oil. Purging (or decontaminating) the oil removes dissolved gases and low boiling-point liquids. You can purge the oil without interrupting system operation, but do not purge it during an acquisition or while the electron multiplier or filament is powered on.
❖
To purge the rotary-vane pump oil
1. Remove the lower panel at the rear side of the ETD Module as described on
2. Set the gas ballast control (See Figure 3-2 on page 3-6
.) to Low
Flow (I).
3. Operate the pump for 10 minutes or until the oil is clear. If the oil remains cloudy or discolored after 10 minutes, replace the oil.
4. Set the gas ballast control to Closed (0), as shown in Figure 3-6
.
High Flow (Position II)
Low Flow (Position I)
Closed (Position 0)
Figure 3-6. Gas ballast control positions
5. Reattach the lower panel at the rear side of the ETD Module.
Changing the Rotary-Vane Pump Oil
You should change the ETD forepump oil every four months (about
3000 hours of operation).
Supplies needed for changing the ETD forepump oil:
•
Rotary-vane pump oil (P/N A0301-15101)
•
Suitable container for removing spent or excess oil
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Note For best results, change the oil while the ETD forepump is still warm after operation. Be careful, however, as the oil can still be very hot at this time.
▲
Warning
Burn Hazard. Handle hot pump oil carefully to avoid being burned or injured.
▲
❖
To change the ETD forepump oil
1. Shut down and vent the Orbitrap Elite ETD mass spectrometer.
Caution
Shut down and unplug the instrument before adding oil.
▲
2. Remove the lower panel at the rear side of the ETD Module as
.
3. Disassemble the rotary-vane pump.
a. Disconnect the foreline vacuum hose. (See Figure 3-2 on page 3-6 .)
b. Disconnect the exhaust vacuum hose.
c. Place the rotary-vane pump on a bench.
Warning
Lifting Hazard. Use the proper lifting technique to lift the
ETD forepump. It weighs approximately 23 kg (50 lb).
▲
4. Drain the spent oil.
a. Remove one of the oil filler plugs.
b. Remove the oil drain plug and allow the oil to drain into a suitable container.
c. Dispose of the spent oil according to local environmental regulations.
d. Replace the oil drain plug.
5. Add fresh oil.
a. Add oil into oil filler reservoir half way between the MIN and
MAX level marks.
b. If the oil level goes above the MAX level mark, remove the drain plug and drain the excess oil from the pump.
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6. Reassemble the rotary-vane pump.
a. Replace the oil filler plug.
b. Return the rotary-vane pump to the floor.
c. Reconnect the foreline vacuum hose to the rotary-vane pump.
d. Reconnect the exhaust vacuum hose to the rotary-vane pump.
e. Plug in the rotary-vane pump.
7. Reattach the lower panel at the rear side of the ETD Module.
8. Plug in the instrument.
9. Restart the system.
Maintenance of the Turbomolecular Pumps
The turbomolecular pumps in the MS portion of the Orbitrap Elite mass spectrometer need maintenance work by the user that is briefly outlined below. In contrast, the turbomolecular pump in the
ETD Module of the Orbitrap Elite ETD mass spectrometer contains no user-serviceable parts.
Note The manuals of the pump manufacturers give detailed advice regarding safety, operation, maintenance, and installation. Please note the warnings and cautions contained in these manuals!
▲
Replacing the Operating Fluid Reservoir of the Turbomolecular Pumps
The manufacturer recommends replacing the operating fluid reservoirs of the turbomolecular pumps at least every four years. The storage stability of the operating fluid is limited. The specification of durability is given by the pump manufacturer. The disposal of used oil is subject to the relevant regulations.
Replacements for the operating fluid reservoirs including Porex rods
(HiPace™ 80: P/N 1275740; HiPace™ 300: P/N 1275730) are available from Thermo Fisher Scientific.
Note The pump bearings have also to be replaced at least every four years. This maintenance operation requires special training and additional equipment. Therefore, Thermo Fisher Scientific recommends calling a Thermo Fisher Scientific field service engineer to replace both the operating fluid reservoir and the pump bearings.
▲
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Maintenance of the ETD Module
This section describes routine ETD Module maintenance procedures that must be carried out to ensure optimum performance of the system.
Some of the procedures describe how to clean components of the
ETD Module. Others involve replacing components or replenishing the electron transfer reagent. See also the ETD Module Hardware Manual for additional information.
illustrates the sequence in which to perform routine maintenance on the ETD system.
Start
Remove
Ion Volume
Clean
Ion Volume
Clean
Ion Source
Lenses
?
No
Replace
Ion Volume
Yes
End
Vent
MS/ETD System
Remove
ETD Module
Covers
Remove
Vacuum Manifold
Cover
Remove
Ion Source from
Vacuum Manifold
Remove
Lenses
Clean
Lenses
Remove
Reagent Ion Source
Block Assembly
Disassemble
Reagent Ion Source
Block Assembly
Clean
Reagent Ion Source
Block Assembly
Reassemble
Reagent Ion Source
Block Assembly
Clean
Reagent Ion Source
Block Assembly
?
No
Yes
Clean
Remaining
Reagent Ion Source
Components
?
No
Yes
Disassemble
Remaining
Reagent Ion Source
Components
Clean
Remaining
Reagent Ion Source
Components
Reassemble
Remaining
Reagent Ion Source
Components
Reinstall
Reagent Ion Source
Block Assembly
Reinstall
Lenses
Reinstall
Reagent Ion Source
Replace
Vacuum Manifold
Cover
Reinstall
ETD Module
Covers
Start Up
MS/ETD System
Figure 3-7. Routine maintenance sequence for ETD system
Table 3-2 on page 3-13 gives advice for correcting frequent problems
with the ETD system.
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Table 3-2. Indications requiring maintenance of the ETD system
Symptom
No ions at m/z 202 with the emission current at the correct level.
The m/z 202 signal intensity drops slowly over several days when the emission current is at the correct level.
A system error message advising that the maximum injection time has been reached for the ETD AGC.
Sudden and complete drop of m/z 202 level, low emission current.
Cause
The m/z 202 is outside the mass range.
Fix
Set the starting mass lower.
The ion volume needs to be cleaned or replaced.
Clean or replace the ion volume when the injection time is over 100 ms. See
The AGC target has not been reached within the specified time limit. The ion volume needs to be cleaned or replaced.
Clean the ion volume. Increase the maximum injection time limit.
The filament may just have blown out.
Check the filament. Replace it if necessary.
See
Handling and Cleaning Reagent Ion Source Parts
A large part of maintaining your reagent ion source consists of making sure that all the components are clean. Use the cleaning procedures listed in this section to clean stainless steel and non-stainless steel parts.
However, use caution when doing so, because some components can be damaged by exposure to liquids.
How often you clean the reagent ion source depends on the amount of reagent introduced into the system. In general, the closer a component is to where the reagent ion is introduced, the more rapidly it becomes
dirty (see the footnote in Table 3-1 on page 3-2 ). For example, the ion
volume needs to be cleaned more often than other parts.
Many parts can be removed and disassembled by hand. Make sure you have all the necessary tools before carrying out a procedure. See below for a list of the tools and supplies generally needed for maintenance of the reagent ion source. Tools should be used only for the maintenance of the reagent ion source and be free of grease or other residues. Handle parts in a manner that maintains their cleanliness.
Note It is crucial that the cleanliness of the parts be maintained when they are handled. Wear gloves and place the parts on surfaces that are clean if the parts are not returned directly to the instrument. If clean surfaces are not available, place the parts on fresh lint free wipes or cloths or aluminum foil that has not been used for any other purpose.
▲
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The following tools and supplies are needed for reagent ion source maintenance:
•
Clean, dry gas (air or nitrogen)
•
New, white nitrile clean room gloves (Fisher Scientific
P/N 19-120-2947B [size medium] or P/N 19-120-2947C [size large]; Thermo Scientific P/N 23827-0008 ) [size medium] or
P/N 23827-0009 [size large])
•
Lint-free cloth or paper
•
Nut driver, 5.5 mm
•
Protective eyewear
•
Screwdriver, Phillips #2
•
Screwdriver, flat blade
•
Wrench, adjustable
•
Wrench, Allen, 2 mm, 2.5 mm, 3 mm, 4 mm, 5/32-in., 5/64-in.,
1/16-in
•
Wrench, open-ended, 1/4-in., 5/16-in., 7/16-in. (2), 1/2-in.,
9/16-in.
•
Wrench, socket, 1/2-in.
Cleaning Stainless Steel Parts
The reagent ion source, ion volume assembly, ion source block, and lenses are made from stainless steel. To clean these parts, follow the procedure described in this topic. Use this procedure with caution because some components can be damaged when exposed to liquids.
The following tools and supplies are needed for cleaning stainless steel parts in the reagent ion source:
•
Acetone, analytical grade (or other suitable solvent)
•
Aluminum oxide abrasive, number 600 (P/N 32000-60340)
•
Applicators, cotton-tipped
•
Beaker, 450 mL
•
Clean, dry gas
•
De-ionized water
•
Detergent (Alconox®, Micro, or equivalent)
•
Dremel® rotary tool or equivalent (recommended)
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•
Foil, aluminum
•
Forceps
•
New, white nitrile clean room gloves
•
Glycerol, reagent grade
•
Lint-free cloth
•
Protective eyewear
•
Tap water
•
Toothbrush, soft
•
Ultrasonic cleaner
Caution
Do not use this procedure to clean ceramic, aluminum, or gold
plated parts. See page 3-17 for the method for cleaning ceramic,
aluminum, or gold plated parts.
▲
Caution
Follow the subsequent instructions precisely. If done wrong, the cleaning procedure could damage the ion source lenses.
▲
Warning
Hand and Eye Hazard. Wear impermeable laboratory gloves and eye protection when performing these cleaning procedures.
▲
❖
To clean reagent ion source stainless steel parts
1. Remove contamination from the surfaces being cleaned.
a. Use a slurry of number 600 aluminum oxide in glycerol and a cleaning brush or cotton-tipped applicator. Contamination often appears as dark or discolored areas, but may not be visible.
The heaviest contamination is usually found around the apertures, such as the electron entrance hole on the ion volume.
b. Clean each part thoroughly, even if no contamination is visible.
c. Use the wooden end of an applicator that is cut at an angle to clean the inside corners.
d. Use a Dremel® tool with the polishing swab at its lowest speed.
This will increase the cleaning efficiency and decrease the time required to clean the part.
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Warning
Injury Hazard. To prevent personal injury, be sure to keep the
Dremel tool away from possible hazards, such as standing water or flammable solvents.
▲
2. Rinse the parts with clean water.
3. Use a clean applicator or toothbrush to remove the aluminum oxide slurry. Do not let the slurry dry on the metal because dried aluminum oxide is difficult to remove.
4. Place the parts in a warm detergent solution in an ultrasonic bath and sonicate them.
a. Make a solution of detergent and warm tap water in a 400 mL glass beaker.
b. Using forceps, place the parts in a beaker containing the warm detergent solution.
c. Place the beaker and contents in an ultrasonic bath for five minutes.
d. Rinse the parts with tap water to remove the detergent.
5. Sonicate the parts in deionized water.
a. Using forceps, place the parts in a beaker containing deionized water.
b. Place the beaker and contents in an ultrasonic bath for five minutes.
c. If the water is cloudy after sonicating, pour off the water, add fresh water, and place the beaker and its contents in a ultrasonic bath again for five minutes. Repeat until the water is clear.
6. Sonicate the parts in acetone.
a. Using forceps, place the parts in a beaker containing fresh acetone.
b. Place the beaker and contents in an ultrasonic bath again for five minutes.
7. Blow-dry the parts immediately. Use clean, dry gas (air or nitrogen) to blow the acetone off the parts.
8. Complete the drying process, doing one of the following:
•
Using forceps, place the parts in a 500 mL glass beaker, cover the beaker with aluminum foil, and put the beaker in an oven set at
100 °C for 30 minutes.
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•
Lay the parts on clean aluminum foil (dull side up) and allow to dry for 30 minutes.
9. Allow the parts to cool before reassembling them.
Cleaning Non-Stainless Steel or Hybrid Parts
To clean the stainless-steel portion of hybrid parts, follow step 1 and step 2 of the instructions on page 3-15 . Perform these steps only on the stainless-steel surfaces of hybrid parts. Do not allow the aluminum oxide slurry to contact the aluminum, ceramic, or gold plated portions of these parts.
Warning
Hand and Eye Hazard. Wear impermeable laboratory gloves and eye protection when performing these cleaning procedures.
▲
The reagent ion source heater ring, filament spacer, lens holder, and spacers are non-stainless steel parts that are made from aluminum, ceramic, or are gold plated.
❖
To clean the non-stainless-steel portions of hybrid parts
1. Scrub all of the parts with a warm detergent solution.
a. Make a solution of detergent and warm tap water in a 500 mL glass beaker.
b. Dip a clean cotton-tipped applicator in the detergent mixture and use the applicator to scrub the parts.
Note Do not soak or sonicate the parts in detergent.
▲ c. Using forceps, rinse the parts thoroughly with tap water to remove the detergent.
Caution
Do not leave aluminum parts, such as the heater ring, in the detergent. Basic solutions, like detergent, damage the surface of aluminum.
▲
2. Rinse the parts in deionized water. Using forceps, dip the parts in a beaker of deionized water. Change the water if it becomes cloudy.
Do not soak or sonicate the parts.
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3. Rinse the parts with acetone. Using forceps, dip the parts in a beaker of acetone. Change the acetone if it becomes cloudy. Do not soak or sonicate the parts.
4. Blow-dry the parts immediately. Use clean, dry gas (air or nitrogen) to blow the acetone off the parts.
Removing the Access Panels
During some ETD Module maintenance activities, it is necessary to remove either the ETD main access panel, or the side access panel, or both (see
Figure 3-8 ). Follow the subsequent procedures to remove these
panels.
7
8
6
9
5
4
3
2
1
Labeled components: 1=ETD main access panel, 2=side access panel, 3=inlet valve knob, 4=inlet valve lever (down is closed, up is open), 5=inlet valve plug, 6, 7, 8, 9=panel fasteners.
Figure 3-8. Rear view of the ETD Module
Removing the ETD Main Access Panel
❖
To remove the ETD main access panel
1. Place the ETD Module to Service mode as directed in
Instrument in Off Condition and Service Mode” on page 3-48 .
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Note In Service mode, all power to the Orbitrap Elite ETD MS electronics is turned off. There are no user accessible components that carry a voltage in this mode. However, the vacuum pumps continue to operate.
▲
Warning
Burn Hazard. The reagent vial heaters can be 108 °C (or set point); the transfer line, the restrictor, and the ion source can be at
160 °C. These components may be too hot to touch. Verify that all of these components are safe to touch before handling them.
▲
Note The ETD main access panel is interlocked with the ETD Module power. When the ETD main access panel is removed, all power to the
ETD Module will be turned off. However, the mechanical pump and turbo pump will continue operating.
▲
2. Remove the inlet valve lever (item 4 in Figure 3-8 on page 3-18
) by pulling it down and away from the ETD Module main access panel.
Do not rotate the lever upwards. It must remain in its down (closed) position to avoid catastrophic venting of the system.
Caution
Rotating the inlet valve lever upwards (to the open position) without the inlet valve plug (item 5 in
Figure 3-8 ) or the ion volume
tool in place will cause a catastrophic venting of the system.
▲
3. Unscrew the inlet valve knob (item 3 in Figure 3-8
) and remove the inlet valve plug (item 5 in
Figure 3-8 ), the inlet valve knob, and the
internal ferrule.
4. Loosen the four panel fasteners (items 6, 7, 8, and 9 in Figure 3-8 ).
5. The top panel rests on hooks pointing into the upward direction.
Tilt the top panel towards you and lift it up and away from the
ETD Module.
Removing the ETD Side Access Panel
❖
To remove the ETD side access panel
1. If it is not already in Service mode, place the ETD Module in
Service mode as directed in
“Placing the Instrument in Off
Condition and Service Mode” on page 3-48
.
Note In Service mode, all power to the Orbitrap Elite ETD MS electronics is turned off. There are no user accessible components that carry a voltage in this mode. However, the vacuum pumps continue to operate.
▲
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Warning
Burn Hazard. The reagent vial heaters can be at 108 °C (or set point); the flow restrictor, the transfer line heaters, and the ion source heater can be at 160 °C. These components may be too hot to touch.
Verify that all of these components are safe to touch before handling them.
▲
Note The ETD side access panel is interlocked with the ETD Module power. When the ETD side access panel is removed, all power to the
ETD Module will be turned off. However, the mechanical pump and turbomolecular pump will continue operating.
▲
2. Using an Allen wrench, loosen the captive screws at the top and remove the screws at the bottom of gray plastic side panel and remove the panel.
3. Using a #2 Phillips screwdriver, loosen the three captive screws on
the metal side access panel (item 2 in Figure 3-8 on page 3-18
) and remove the panel.
Warning
Burn Hazard. Reagent vial heaters, ion source heater, flow restrictor, and transfer lines are accessible under the ETD side access panel. These are heated components. Verify that they are safe to touch before handling them.
▲
Replace the panels by following the above steps in reverse order and reversing the instructions in each step.
Maintenance of the Reagent Ion Source
The reagent ion source consists of an ion volume, filament, and ion source lenses. Because the ion volume is exposed directly to samples introduced into the reagent ion source, it requires the most frequent cleaning. You can access the ion volume assembly with or without an inlet valve.
To restore system performance, always clean the ion volume first, then the ion source lenses. If cleaning either of these components does not restore system performance, try cleaning the entire reagent ion source.
This section contains these maintenance procedures:
•
“Cleaning the Ion Volume With an Inlet Valve” on page 3-21
•
“Cleaning the Ion Source Lens Assembly” on page 3-33
•
“Cleaning the Ion Source Block” on page 3-40
•
“Replacing the Ion Source Filament” on page 3-42
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•
“Replacing Inlet Valve Components” on page 3-45
The ion source, the ion trap, and their components are shown in
2
3
4
1
6
5
Labeled components: 1=ion source lenses, 2=filament assembly, 3=ion source block, 4=magnet support, 5=magnets, 6=ion volume (inside the ion source block, 3)
Figure 3-9. Ion source components (left view)
Cleaning the Ion Volume With an Inlet Valve
The ion volume is where molecules interact with energetic electrons to form ions. Because the ion volume is exposed directly to reagents introduced into the reagent ion source, you will have to clean it more frequently than other parts. How often you have to clean the ion volume assembly will depend on the types and amounts of reagents used.
For cleaning the ion volume with an inlet valve, the following tools and supplies are needed:
•
Cleaning supplies for stainless steel parts (See “Cleaning Stainless
•
Gloves (clean, lint-free, and powder-free)
•
Ion volume tool and guide bar
•
Lint-free cloth
Using the ion volume tool allows you to access the ion volume by entering the vacuum manifold through the inlet valve without venting the instrument.
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On
On/Standby button
Off Standby
❖
To clean the ion volume with an inlet valve
1. Click the On/Standby button in the Tune Plus window to place the
Orbitrap Elite ETD mass spectrometer in Standby mode. See
.
Reagent Ion Source instrument control icon
Status View
Figure 3-10. Tune Plus window (Orbitrap Elite ETD)
2. Open the Reagent Ion Source dialog box (
) in Tune Plus by clicking the Reagent Ion Source instrument control icon.
3. Place the guide bar handle (item 13 in
) to the 3 o’clock position (
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1
2
3
4
5
12
11
10
9
8
13
7
6
Labeled components: 1=alignment line, 2=lock position, 3=unlock position,
4=ion volume tool, 5=bayonet lock, 6=second stop, 7=guide ball track, 8=first stop, 9=guide bar, 10=guide ball hole, 11=guide ball, 12=ion volume tool handle, 13=guide bar handle
Figure 3-11. Ion volume tool components
4. Insert the guide bar (item 9 in Figure 3-11
) into the guide bar opening in the back of the ETD Module (
Inlet valve lever in down (closed) position
Guide bar handle Guide bar opening
Figure 3-12. Guide bar being inserted into guide bar opening a a
Guide bar handle is facing to the right. The inlet valve is closed when the inlet valve lever is in the down position and open when it is in the up position.
5. Push the guide bar in as far as it will go, then rotate it 90° clockwise to lock in the guide bar (
Figure 3-13 on page 3-24 ). The guide bar
handle faces the floor at the completion of this step.
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Inlet valve lever in down (closed) position
Guide bar handle
Figure 3-13. Guide bar insertion complete a a
Guide bar handle is facing the floor. The inlet valve is closed when the inlet valve lever is in the down position and open when it is in the up position.
6. Prepare the inlet valve and ion volume tool for insertion.
3
4
5
1
2
Labeled components: 1=inlet valve knob, 2=inlet valve plug, 3=inlet valve lever (down is closed, up is open), 4= guide bar opening, 5=main access panel
Figure 3-14. Rear view of the ETD Module, showing the inlet valve
Make sure the inlet valve is closed (inlet valve lever is down, as shown in
) and remove the inlet valve plug (item 2 in
). Do this by rotating (loosening) the inlet valve knob
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Maintenance of the ETD Module
(item 1 in Figure 3-14 on page 3-24
) until the inlet valve plug will slide out easily. The inlet valve plug prevents air from entering the vacuum manifold in case the inlet valve is inadvertently opened.
7. Turn the ion volume tool handle to the unlock position, which indicates that the ion volume tool is in position to accept the ion
.
Thermo Fisher Scientific
Figure 3-15. Ion volume tool handle in the unlock position
8. Insert the ion volume tool and evacuate the inlet valve: a. Insert the guide ball into the guide ball hole.
5
7
1
2
6
4
3
Labeled components: 1=ion volume tool entry housing, 2=inlet valve opening,
3=first stop, 4=guide bar, 5=ion volume tool, 6=inlet valve lever, 7=inlet valve knob
Figure 3-16. Ion volume tool guide bar first stop
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Maintenance of the ETD Module b. Slide the ion volume tool forward in the guide bar track until the guide ball is at the guide bar’s first stop, which is shown in
and
c. Slide the ion volume tool so the guide ball is in the groove at the first stop (
). This prevents the probe from being pulled forward when the inlet valve is evacuated.
d. Tighten the inlet valve knob ( Figure 3-16 ) to ensure that a
leak-tight seal is made.
e. Click Open Probe Interlock in the Reagent Ion Source dialog
box ( Figure 3-17 ). A message box appears stating that the probe
interlock is being pumped down. The target pressure is
<0.1 mTorr. If a pressure of 0.1 mTorr or less is not obtained, replace the inlet valve seal as described in
. When the target pressure is achieved, a message appears stating that the ball valve can be opened (
).
Open Probe Interlock
Figure 3-17. Reagent Ion Source dialog box, Open Probe Interlock button.
Figure 3-18. Instrument Message box: The Ball Valve can now be opened
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Maintenance of the ETD Module f. Once evacuation is complete, push up the inlet valve lever to open the inlet valve (
9. Remove the ion volume: a. Slide the ion volume tool into the vacuum manifold until the tip of the ion volume tool is fully inserted into the ion volume holder, as shown in
1
6
5
2
4
3
Labeled components: 1=ion volume tool, 2=inlet valve opening, 3=guide bar,
4=second stop, 5=inlet valve lever in open (up) position, 6=inlet valve knob
Figure 3-19. Ion volume tool inserted into the inlet valve
Thermo Fisher Scientific second mark second stop first mark Guide Ball
Figure 3-20. Detail of ion volume tool fully inserted into the inlet valve
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You will know that the ion volume tool is fully inserted into the
ion volume holder because the guide ball (item #11, Figure 3-11 on page 3-23
) will be just past the first mark on the guide bar as shown in
b. Turn the ion volume tool handle counterclockwise to the lock position, See
Figure 3-21 . Listen for a click indicating that the
handle is fully engaged in the lock position and is holding the ion volume.
Figure 3-21. Ion volume tool handle in the locked position c. Withdraw the ion volume tool (the ion volume is attached) until the guide ball reaches the first stop (see
Figure 3-16 on page 3-25 for the first stop
position).
d. Close the inlet valve by pushing the lever down.
Caution
Do not withdraw the ion volume tool beyond the point where the guide ball reaches the first stop in the guide bar. Close the inlet valve before withdrawing the ion volume tool past the first stop. Otherwise, the system will vent to the atmosphere and cleaning the components that are under vacuum will be required.
▲
e. Loosen the inlet valve knob ( Figure 3-19 on page 3-27
).
f. Continue withdrawing the ion volume tool completely from the inlet valve by sliding the ion volume tool through the guide ball track in the guide bar.
Warning
Burn Hazard. The ion volume will be too hot to touch. Let it cool to room temperature before handling it.
▲
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10. Clean the ion volume: a. Turn the ion volume tool handle to the unlock position
). The ion volume tool handle unlock position icon is shown at the left.
b. Remove the ion volume from the ion volume tool. Using clean gloves, press the ion volume into the tip of the ion volume tool and rotate it to disconnect the bayonet pins from the pin guides.
Pull the ion volume out of the ion volume tool, as illustrated in
Bayonet Pin
Bayonet Pin Guide
Ion Volume Tool
Ion Volume Holder
Spring Washer
Ion Volume
Figure 3-22. Ion volume assembly c. Press the ion volume into the ion volume holder and rotate the ion volume to remove it from the ion volume holder See
Thermo Fisher Scientific
Figure 3-23. Separating ion volume and ion volume holder d. Clean ion volume and ion volume holder according to the instructions in
“Cleaning Stainless Steel Parts” on page 3-14 .
❖
To reinsert the ion volume
1. Press the ion volume into the ion volume holder and rotate the ion volume to secure it to the ion volume holder.
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2. Place the clean ion volume on the ion volume tool: a. Place the ion volume into the bayonet lock located on the ion volume tool. Make sure that the alignment arrows on the ion volume and ion volume tool are facing each other. See
.
Caution
To avoid damage to the ion source, ensure that the arrows on the ion volume tool and ion volume are aligned.
▲
4
1
2
3
5
6
7
Labeled components: 1=ion volume alignment arrow, 2=bayonet pin,
3=bayonet lock, 4=ion volume tool alignment arrow, 5=bayonet guide, 6=ion volume tool, 7=ion volume
Figure 3-24. Placing the ion volume on the ion volume tool
Note Wear clean, lint-free, and powder-free gloves when you handle parts inside the vacuum manifold.
▲ b. Turn the ion volume tool handle to the lock position. (See
.)
3. Insert the ion volume tool and evacuate the inlet valve: a. Insert the guide ball into the guide ball hole and slide the ion volume tool forward in the guide bar track until the guide ball is at the guide bar’s first stop (see
).
b. Turn the ion volume tool so that the guide ball is in the groove at the first stop (
Figure 3-16 on page 3-25 ). This prevents the
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Maintenance of the ETD Module probe from being pulled forward when the inlet valve is evacuated.
c. Tighten the inlet valve knob to ensure a leak-tight seal
).
d. Click Open Probe Interlock in the Reagent Ion Source dialog
box ( Figure 3-17 on page 3-26
). A message box will appear stating that the probe interlock is being pumped down. The target pressure is <0.1 mTorr. If a pressure of 0.1 mTorr or less is not obtained, the inlet valve seal must be replaced as described
in “Replacing Inlet Valve Components” on page 3-45 . When the
target pressure is achieved, a message will appear stating that the ball valve can be opened. See
e. Once evacuation is complete, push the inlet valve lever up to open the inlet valve. See
4. Reinsert the ion volume: a. Slide the ion volume tool into the vacuum manifold, as illustrated in
b. Listen for a click indicating that the ion volume has connected with the ion source block. The guide ball will be slightly beyond the second stop on the guide bar. See
c. Turn the ion volume tool handle to the unlock position. See
Thermo Fisher Scientific
Figure 3-25. Ion volume tool handle in the unlock position i.
Withdraw the ion volume tool away from the ion volume about 2.5 cm (1 in) and turn the ion volume tool handle to
the lock position. See Figure 3-26 on page 3-32
.
ii. Slide the ion volume tool back into the vacuum manifold until the end of the ion volume tool just touches the ion volume.
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Maintenance of the ETD Module iii. If the ion volume tool does not go into the inlet valve completely, the ion volume is not seated properly.
d. Withdraw the ion volume tool until the guide ball reaches the first stop (see
Figure 3-26. Ion volume tool handle in the locked position e. Close the inlet valve by pushing down on the inlet valve lever
(
Caution
Do not withdraw the ion volume tool beyond the point where the guide ball reaches the first stop in the guide bar. Close the inlet valve before withdrawing the ion volume tool past the first stop. Otherwise, the system vents to the atmosphere.
▲
f. Loosen the inlet valve knob (item 6 in Figure 3-19 on page 3-27 ).
g. Continue withdrawing the ion volume tool completely from the inlet valve by sliding the ion volume tool through the guide ball track in the guide bar.
5. Remove the ion volume tool and guide bar from the vacuum manifold: a. Remove the guide bar by rotating it 90 degrees counter-clockwise and sliding it out of the entry housing.
b. Replace the inlet valve plug and tighten the inlet valve knob
(item 6 in
c. Click Close in the message stating that the ball valve can be opened. (See
6. Re-tune the MS detector.
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Maintenance of the ETD Module
Note Tune Plus provides an evaluation procedure for CI gas pressure under Diagnostics > Diagnostics > Tools > System evaluation >
Reagent CI gas pressure evaluation. Thermo Fisher Scientific recommends performing this procedure after replacing the filament and/or the ion volume.
▲
Cleaning the Ion Source Lens Assembly
If cleaning the ion volume did not restore system performance, try cleaning the ion source lens assembly. The ion source lens assembly comes in direct contact with reagent ions introduced into the
ETD Module and needs to be cleaned periodically (though not as often as the ion volume).
❖
To clean the ion source lens assembly
1. Prepare the ETD Module for maintenance: a. Prepare a clean work area by covering the area with a clean lint-free cloth.
b. Shut down and vent the ETD Module. (See
“Shutting Down the Instrument” on page 2-7 .)
Caution
Shut down and unplug the Orbitrap Elite ETD mass spectrometer before proceeding with the next steps of this procedure.
▲
Note Wear clean, lint- and powder-free gloves when you handle parts inside the vacuum manifold.
▲
Warning
Burn Hazard. The ion source may be too hot to touch even if the cooling nitrogen has completed its cycle. Be sure that the ion source has cooled to room temperature before handling it.
▲
2. Remove the ion source assembly: a. Remove the main access panel of the ETD Module (item 1 in
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Caution
It is good practice to keep the inlet valve lever in the down
(closed) position whenever it is not explicitly required to be in the up position (open), even if the vacuum manifold is at atmospheric pressure.
This is to be consistent with maintenance procedures that rely on the inlet valve lever being closed at the appropriate step to prevent the accidental loss of vacuum. If the vacuum is accidently lost the system may be damaged. At a minimum, the components that were under vacuum might have to be cleaned.
▲
Labeled components: 1=inlet valve plug, 2=vacuum manifold, 3=inlet valve lever, 4=guide bar, 5=entry housing, 6=inlet valve knob, 7=inlet valve block,
8=foreline hose connection, 9=12 pin feedthrough, 10=vacuum manifold probe plate, 11=ball valve housing, 12= inlet valve solenoid
Figure 3-27. Inlet valve components (ion volume tool not shown) b. Remove all connectors between the components on the vacuum manifold probe plate (item #10,
Figure 3-27 on page 3-34 ) and
the ETD Control PCB (
c. Remove the valve shield from the vacuum manifold probe plate
(
Figure 3-28 ) by loosening the four screws at the corners of the
shield.
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Maintenance of the ETD Module
1
Figure 3-28. Valve shield (1) covering the vacuum manifold probe plate d. Remove the foreline hose on the source from its connection
).
Thermo Fisher Scientific
Figure 3-29. Removing the foreline hose from its connection e. Remove the four screws holding the vacuum manifold probe
and item 10 in Figure 3-27 on page 3-34
).
Support the plate with your hand as shown in
Arrows point to the four hex screw locations (items 1–4).
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1 2
4 (at lower left corner of manifold probe plate)
3
Figure 3-30. Unscrewing the vacuum manifold probe plate f. Remove the vacuum manifold probe plate (
Figure 3-31. Removing the vacuum manifold probe plate g. Unplug the 12-pin feedthrough harness from the feedthrough
(
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Maintenance of the ETD Module
4 2
Labeled components: 1=unplugged 12 pin feedthrough, 2=thumbscrews,
3=transfer line bellows, 4=ion source assembly
Figure 3-32. Interior of vacuum manifold h. Remove the ion source assembly from the vacuum manifold
) by first loosening the ion source thumbscrews (item 2 in
i.
Second, as you remove the ion source assembly (item 1 in
Figure 3-33 ) gently shift it to the left (arrow 2 in Figure 3-33
) before and while pulling it out. This will allow the ion source assembly to disengage from the transfer line bellows (item 3 in
Figure 3-33 ) as it is removed. Alternatively, gently depress the
transfer line bellows (
Figure 3-32 on page 3-37 ) to disengage it
from the ion source assembly.
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1 3
2
Figure 3-33. Removing the ion source assembly from the vacuum manifold a a
The ion source assembly (item 1) is gently shifted to the left (arrow 2) to allow the ion source assembly to disengage from the transfer line bellows (item 3) as it is removed.
The ion source assembly is held together with a clip (item 8 in
). However, it is necessary to keep the tips of your gloved fingers on both the front edge of the ceramic lens holder
(item 11 in
Figure 3-34 ) and the back of the magnet yoke (item 3 in
) when you handle the ion source assembly. This prevents unsecured components inside of the ceramic lens holder from falling out.
Caution
When handling the ion source assembly, it is important to handle it with gentle finger pressure on each end (as instructed above) to keep unsecured components from falling out of the assembly.
▲
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Thermo Fisher Scientific
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11
10 1 2 3 5 9 8 7
Labeled components: 1=thumbscrew, 2=springs, 3=magnet yoke, 4=Ion
Source PCB, 5=magnets, 6=ion source block, 7=ceramic lens holder, 8=spring clip, 9=spring clip thumb screw, 10=12 pin feedthrough harness, 11=finger over front edge of ceramic lens holder to keep unsecured components from falling out of the ion source assembly.
Figure 3-34. Ion source assembly
An exploded view of the ion source assembly is shown in Figure 3-35
.
5 4 3
2
1
8 7 6 5 3
Labeled components: 1=thumbscrews, 2=springs, 3=E-clips , 4=magnet yoke,
5=magnets, 6=ion source, 7=ion source lens assembly, 8=ceramic lens holder
Figure 3-35. Ion source assembly exploded view
3. Separate the magnet yoke and the ion source.
4. Remove the ion source lens assembly from the ceramic lens holder
).
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5. Clean the ion source lens assembly according to the procedure in
“Cleaning Stainless Steel Parts” on page 3-14 . Pay particular
attention to the areas inside the tube and around the holes in the lens assembly.
6. Replace the ion source assembly: a. Insert the lens assembly into the ceramic lens holder.
b. Reassemble the ion source assembly.
c. Reinstall the ion source assembly into the vacuum manifold by following step 2 in reverse order.
7. Restore the ETD Module to operational status. See
System after a Shutdown” on page 2-9
.
Cleaning the Ion Source Block
If cleaning the ion volume and ion source lens assembly does not restore system performance, you might need to clean the ion source block.
Generally, you need to clean the ion source block no more than once every six months.
Supplies needed for cleaning the ion source:
•
Cleaning supplies
•
Gloves (clean, lint-free, and powder-free)
•
Lint-free cloth
❖
To clean the ion source block
1. Prepare the ETD Module for maintenance: a. Prepare a clean work area by covering the area with lint-free cloth.
b. Shut down and vent the Orbitrap Elite ETD mass spectrometer.
(See “Shutting Down the Orbitrap Elite Mass Spectrometer
Caution
Shut down and unplug the Orbitrap Elite ETD mass spectrometer before proceeding with the next steps of this procedure.
▲ c. Remove the ion source assembly by following the procedures in step 2
in “Cleaning the Ion Source Lens Assembly” on page 3-33 .
Note Wear clean, lint- and powder-free gloves when you handle parts inside the vacuum manifold.
▲
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Maintenance of the ETD Module
2. Disassemble the ion source assembly ( Figure 3-34 on page 3-39 and
Figure 3-35 on page 3-39 ), remove and disassemble the ion source
).
a. Remove the magnet yoke and the ion source block with the ion source lens assembly.
b. Remove the ion source lens assembly.
c. Remove the ion source.
1
3
2
14
13
12
9
5
4
2
6
8
7
10
11
Labeled components: 1=ion source filament, 2=cartridge heaters, 3=base studs (3×), 4=Ion Source PCB, 5=temperature sensor, 6=ion source block,
7=ion volume key thumbscrew, 8=ion volume pin, 9=ion volume, 10=spring clip, 11=spring clip thumb screw, 12=heater ring, 13=sample inlet aperture (in side of item 6), 14=ceramic spacer
Figure 3-36. Ion source, exploded view
d. Remove the three base studs (item 3 in Figure 3-36
). Be careful not to damage the leads on the Ion Source PCB (item 4 in
e. Gently remove the Ion Source PCB (item 4 in
the ion source by loosening the spring clip thumbscrew (item 11 in
Figure 3-36 ) and the spring clip (item 10 in Figure 3-36 ) and
sliding the three cartridge heaters and the temperature sensor
(items 2 and 5 in
filament connectors on the Ion Source PCB. Do not bend or twist the cartridge heaters or temperature sensor.
f. Remove the filament and ceramic spacer (items 15 and 1 in
Figure 3-36 ) from the ion source block (item 6 in Figure 3-36 ).
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g. Remove the ion volume key thumbscrew (item 7 in Figure 3-36 on page 3-41
).
Note It is not necessary to remove the ion volume pin (item 8 in
Figure 3-36 ). If you remove it, you should reinsert it just far enough so
the ball will keep an ion volume (item 9 in
out. If the ball extends too far, the ion volume will be difficult to remove.
▲
3. Clean the ion source parts and replace the ion source assembly: a. Clean each component of the ion source, as described in
“Cleaning Stainless Steel Parts” on page 3-14 and
Non-Stainless Steel or Hybrid Parts” on page 3-17
.
b. Reassemble the ion source block.
c. Reassemble the ion source assembly.
d. Reinstall the ion source assembly into the vacuum manifold by following step 2
of “Cleaning the Ion Source Lens Assembly” on page 3-33 in reverse order.
4. Restore the ETD Module to operational status. See
System after a Shutdown” on page 2-9
.
Replacing the Ion Source Filament
The number of ions produced in the ion source is approximately proportional to the filament emission current. If you notice that ion production is low, this might indicate that the filament has failed and needs to be replaced. If the measured emission current is substantially less than the value that the emission current is set to, or if the measured emission current is decreasing over time, then the filament has failed or is failing and needs to be replaced. The ion source filament assembly is shown in
Supplies needed for replacing the ion source filament:
•
Filament Assembly DSQ II (P/N 120320-0030)
•
Gloves, clean, lint-free, and powder-free
•
Protective eyewear
•
Lint-free cloth
•
Forceps or dental pick
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5
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3
4
1
2
Labeled components: 1=ion source lens assembly, 2=ion source block, 3=Ion
Source PCB, 4=base studs (3x), 5=ion source filament
Figure 3-37. Ion source lens assembly and ion source
❖
To replace the ion source filament
1. Prepare the ETD Module for maintenance.
a. Prepare a clean work area by covering the area with lint-free cloth.
b. Shut down and vent the Orbitrap Elite ETD mass spectrometer.
(See
“Shutting Down the Orbitrap Elite Mass Spectrometer
.)
Caution
Shut down and unplug the Orbitrap Elite ETD mass spectrometer before proceeding with the next steps of this procedure.
▲ c. Remove the ion source assembly by following the procedures in step 2 in
“Cleaning the Ion Source Lens Assembly” on page 3-33
.
Note Wear clean, lint- and powder-free gloves when you handle parts inside the vacuum manifold.
▲
2. Disassemble the ion source assembly ( Figure 3-34 on page 3-39 and
Figure 3-35 on page 3-39 ), remove and disassemble the ion source
).
a. Remove the ion source lens assembly (item 1 in Figure 3-37
).
b. Remove the three base-studs (item 3 in Figure 3-36
, item 4 in
c. Remove the filament assembly (items 1 and 15 in
,
item 5 in Figure 3-37 ) and ion source block (item 2 in
Figure 3-37 ) from the three filament connectors and cartridge
heaters (item 2 in
Figure 3-36 ) on the Ion Source PCB (item 4
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Maintenance of the ETD Module in
Figure 3-36 ) according to the procedure in step e
of
“Cleaning the Ion Source Block“ on
Note Now is a good time to clean the ion volume and ion source lenses.
▲
3. Inspect and install a new filament assembly: a. Turn the filament assembly over and, using a strong light and a magnifying glass, verify that the filament wire is centered in the electron lens hole. If necessary, carefully use forceps (or a dental pick) to adjust the filament wire.
Figure 3-38 shows the centered
filament wire as seen from the bottom of the filament through the electron lens hole.
Note A bent filament can lead to a low or absent anion signal. If the filament wire is bent, not centered, or otherwise damaged, you must replace the filament assembly.
▲
Centered wire
Direction of light source
Figure 3-38. Filament wire as seen from the bottom of the filament through the electron lens hole b. Insert the filament into the ceramic spacer of the ion source
block (item 14 in Figure 3-36 on page 3-41
).
c. Align the filament leads with the Ion Source PCB connectors and gently press the leads into the connectors. Normally, there is a small gap (about 0.020 in or 0.50 mm) between the filament and the connectors. The gap allows the ceramic filament centering ring (spacer) to properly position and align the electron lens hole with the ion volume.
d. Reinstall the three base-studs (item 3 in
, item 4 in
).
4. Reassemble ion source and ion source assembly.
5. Insert the ion source assembly into the vacuum manifold.
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6. Restore the ETD Module to operational status. See “Starting Up the
System after a Shutdown” on page 2-9 .
Note Tune Plus provides an evaluation procedure for CI gas pressure under Diagnostics > Diagnostics > Tools > System evaluation >
Reagent CI gas pressure evaluation. Thermo Fisher Scientific recommends performing this procedure after replacing the filament and/or the ion volume.
▲
Replacing Inlet Valve Components
This topic provides the procedure for replacing inlet valve components.
Perform this procedure when the inlet valve seal or the inlet valve is being replaced.
Tools and supplies needed for replacing inlet valve components:
•
Inlet Valve Seal Kit (P/N 119265-0003)
•
Lint-free cloth
•
Wrench, open-ended, 5/16-in
•
Wrench, Allen, 4 mm
❖
To replace inlet valve components
1. Pull down the inlet valve lever to close the inlet valve. See
Inlet valve knob
Thermo Fisher Scientific
Inlet valve plug
Inlet valve lever in the down (closed) position
Guide bar opening
Figure 3-39. Inlet valve components
2. Rotate the inlet valve knob counter-clockwise until you can easily remove the inlet valve plug.
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3. Continue to rotate the valve knob until you can remove it.
The inlet valve knob has a stainless-steel ferrule inside. Keep the inlet valve knob and ferrule together.
4. Pull out the knob on the inlet valve seal tool (P/N 119283-0001)
1
, so that the knob is loose. See
.
Knob
Figure 3-40. Inlet valve seal tool
5. Insert the tool straight into the inlet valve. See
Figure 3-41. Inlet valve seal tool inserted in the inlet valve
Caution
Do not scratch the surface of the seal. Use only the inlet valve seal tool to remove or install an inlet valve seal.
▲
6. Press in the knob on the tool until it stops.
7. Remove the tool. The inlet valve seal should be on the tool. See
.
1
Item contained in Inlet Valve Seal Kit (P/N 119265-0003).
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Figure 3-42. Inlet valve seal on the inlet valve seal tool
8. Loosen the knob to disengage the seal. See
Inlet valve seal with O-rings
Inlet valve seal
Figure 3-43. Inlet valve seal disengaged from tool
9. Discard the seal with its O-rings in place.
10. Place one O-ring (P/N 3814-6530)
1
into each of the two slots on the inlet valve seal (P/N 119683-0100)
11. Place the new inlet valve seal onto the inlet valve seal tool.
12. Insert the inlet valve seal tool into the inlet valve into it stops.
13. Remove the tool. The O-rings on the valve seal secure the inlet valve seal in the opening.
14. Reinstall the ferrule, knob, and plug into the inlet valve opening.
Thermo Fisher Scientific
1
Item contained in Inlet Valve Seal Kit (P/N 119265-0003).
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Replacing the Reagent Vials
A significant drop of the m/z 202 signal within one hour with the mission current at the correct level indicates a low reagent supply. In this case, you should replace the fluoranthene vial.
Changing the reagent vials requires that the Orbitrap Elite ETD mass spectrometer be placed in Service mode after the vials have cooled. (Vial cooling is done in Off condition.) See the following sections for procedures to be used to change the reagent vials:
•
“Placing the Instrument in Off Condition and Service Mode” on page 3-48
•
“Installing/Exchanging the Reagent Vials” on page 3-51
The ETD reagent vials are designed to keep the ETD reagent out the lab environment. Removal and replacement of the ETD reagent vials when they are not empty causes excessive puncturing of the septums and reduces their integrity. This could result in ETD reagent (fluoranthene) entering the lab environment. Prevent this from occurring by removing and replacing the ETD reagent vials only when they are empty.
Caution
To preserve the integrity of the ETD reagent vial septums, remove and replace the ETD reagent vials only when they are empty. Do not reinstall used vials.
▲
Note Store and handle all chemicals in accordance with standard safety procedures. The Material Safety Data Sheet (MSDS) describing the chemicals being used should be freely available to lab personnel for them to examine at any time. Material Safety Data Sheets (MSDSs) provide summarized information on the hazard and toxicity of specific chemical compounds.
MSDSs also provide information on the proper handling of compounds, first aid for accidental exposure, and procedures for cleaning spills or dealing with leaks. Producers and suppliers of chemical compounds are required by law to provide their customers with the most current health and safety information in the form of an MSDS.
Read the MSDS for each chemical you use. Dispose of all laboratory reagents in the appropriate manner (see the MSDS).
▲
Safety information about fluoranthene is given in
Placing the Instrument in Off Condition and Service Mode
The power switches control power to the Orbitrap Elite ETD mass spectrometer (MS and ETD Module). The ETD Module power switches control the power to the ETD Module only. When the
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
User Maintenance
Maintenance of the ETD Module
Orbitrap Elite ETD MS is fully operational (all systems On), the MS
Main Power switch is in the On position and the FT Electronics switch is in the operating (On) position.
Normally, the ETD Module Power and Service switches remain On. Use the FT Electronics switch on the MS unit to place the Orbitrap
Elite ETD mass spectrometer in Service mode. Turn On and Off the instrument (both ETD Module and MS) with the MS Main Power switch.
Warning
Burn Hazard. When mass spectrometer and ETD Module system are turned On, the flow restrictor, the transfer line heaters, and the ion source heater can be at 160 °C. The vial heaters can be at 108 °C
(or set point). Do not attempt to replace reagent vials or to service heated components until you have determined that they have cooled to a safe temperature for handling.
▲
On Off
Note The instructions that follow assume that no analyte is flowing into the API source.
▲
Standby
❖
To place the Orbitrap Elite ETD mass spectrometer in Off Condition and
Service mode and to verify that the vials are safe to handle
1. If the Tune Plus window is not already open, choose Start >
Programs > Thermo Instruments > LTQ > LTQ Tune from the taskbar. The Tune Plus window appears. (See
.)
You can determine the state of the MS detector by observing the state of the On/Standby button on the Control/Scan Mode toolbar.
The three different states of the On/Standby button are shown at the left.
2. Choose Control > Off from the Tune Plus pull-down menu to place the system in Off condition. When the mass spectrometer is in Off condition, the Orbitrap Elite ETD MS turns off the ion source sheath gas, auxiliary gas, high voltage, and all of the ETD Module heaters.
Note It is important to choose Control > Off from the Tune Plus pull-down menu in order to shut down all of the ETD Module heaters.
▲
3. Click the reagent ion source portion of the instrument control icon at the top of the Tune Plus window. The Reagent Ion Source dialog
).
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3-49
User Maintenance
Maintenance of the ETD Module
Observe the temperature of Vial 1 in the Actual column of the
Reagent Ion Source dialog box ( Figure 3-44
). Nitrogen cooling gas
will flow until the vial reaches 70 °C. (See “Turning Off the Reagent
Ion Source: What to Expect” on page 2-14
.) Allow up to 90 minutes for the vial temperature to reach ambient temperature (about
30 °C).
actual vial temperature in °C
Figure 3-44. Reagent Ion Source dialog box
Warning
Burn Hazard. Do not attempt to handle the vials or vial holders when the cooling nitrogen stops. They are still too hot to handle when the cooling nitrogen stops at a vial temperature of 70 °C.
Allow the vials to cool to about 30 °C (allow up to 90 minutes after the cooling gas stops) before proceeding with the next step and handling the vials.
▲
4. Toggle the FT Electronics switch to Service mode (Off ) when the vial has reached a temperature that is safe for handling (about
30 °C). Toggling the FT Electronics switch to Service mode turns off all components except the turbomolecular pumps and the forepumps in both the mass spectrometer and the ETD Module.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
User Maintenance
Maintenance of the ETD Module
Note Do not place the ETD Module Service switch into its Service mode (Off ) position while the MS switches are left in their On positions. This could cause communication problems between the MS and the ETD Module. The ability to control the Service mode for both the MS and the ETD Module at one point (at the FT Electronics switch) is a safety feature.
▲
Warning
Burn Hazard. Do not place the system in Service mode until the vials reach a safe temperature (about 30 °C). System temperature monitoring will stop when the system is placed in Service mode. Do not attempt to handle the vials, the vial holders, or the heater assembly until a safe temperature is reached (about 30 °C).
▲
The Orbitrap Elite ETD mass spectrometer is now in Service mode and the vials are at a safe temperature for handling.
Installing/Exchanging the Reagent Vials
After the reagent vial heaters have cooled to room temperature, the reagent vials are ready to be installed or exchanged.
❖
To install or exchange the reagent vials
1. Remove the back panel from the ETD Module. (See
ETD Main Access Panel” on page 3-18
.) This exposes the reagent
inlet source heating unit, which has its own cover ( Figure 3-45 on page 3-52
).
Warning
Burn Hazard. Follow the procedures described in “Placing the
Instrument in Off Condition and Service Mode” on page 3-48 before
removing the back panel of the ETD Module. Removing the back panel before the system is placed in Service mode will open the panel electrical interlocks and stop all system activity including temperature monitoring. In the absence of temperature monitoring, you might attempt to handle the vials before it is safe to do so.
▲
2. Make sure that the vial heater cover is cool to the touch.
Warning
Burn Hazard. The vial heaters can be at 108 °C (or set point).
Allow sufficient time for the vials to cool (up to 90 minutes) and then
place the system in Service mode. (See “Placing the Instrument in Off
Condition and Service Mode” on page 3-48.) Verify that the vial heater
cover is safe to handle before attempting to remove the vial holders and reagent vials.
▲
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
3-51
User Maintenance
Maintenance of the ETD Module
Reagent Inlet
Source Unit
Vial Heater Cover
Figure 3-45. ETD Module with back panel removed
3. Put on a pair of new, white nitrile clean room gloves and protective eye wear.
4. Using a Phillips screwdriver, remove the screws in the vial heater cover. The vial heater cover is located on the right side of the
ETD Module as you view it from the back of the Orbitrap
Elite ETD mass spectrometer ( Figure 3-45 ).
5. Remove the vial holder by gently pulling it out of the vial heater.
6. Remove the empty vial if it is present. The vial holder is a cylindrical tube with a handling knob at one end and ribs along its length. See
. These ribs prevent the vial holder from rotating once it
is placed into the vial heater. Figure 3-47 on page 3-53 shows the tab
and ribs of a vial holder in the vial heater.
Note Dispose of an empty fluoranthene vial in accordance with its
MSDS.
▲
Warning Avoid exposure to potentially harmful materials.
Always wear protective gloves and safety glasses when you handle solvents or corrosives. Also contain waste streams and use proper ventilation. Refer to your supplier's Material Safety Data Sheet (MSDS) for proper handling of a particular compound.
▲
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User Maintenance
Maintenance of the ETD Module
Figure 3-46. Reagent vials with holders
7. Take a vial containing the ETD reagent (fluoranthene) from its box and remove the aluminum tab from the top of the vial’s crimp seal.
8. Put the vial into a vial holder.
Vial Heater ribs
Vial 1 Heater
Vial Holder Knob
Vial 2 Heater
Figure 3-47. ETD Module with vial heater cover removed
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User Maintenance
Maintenance of the ETD Module
9. Place this ETD reagent vial and its vial holder into the Vial 1 heater
(top vial heater). Gently slide the vial holder into the vial heater.
10. Place the empty vial from the box into the other vial holder if an empty vial is not already installed.
11. Place this empty vial and its vial holder into the Vial 2 heater
(bottom vial heater) if an empty vial is not already installed.
Warning
Health Hazard. The empty vial in the Vial 2 heater is an integral part of the carrier/CI gas system. It is necessary to keep the carrier/CI gas system closed to the laboratory. If no vial is placed in the
Vial 2 heater, the carrier/CI gas containing the reagent might escape to the laboratory causing a safety problem.
▲
Caution
If no vial is placed in the Vial 2 heater, the ETD Module will not operate correctly and the filament will burn out.
▲
12. Reinstall the vial heater cover over the vial heaters.
13. Reinstall the back panel of the ETD Module. See “Removing the
ETD Main Access Panel” on page 3-18 . The ETD Module will not
turn on unless the back panel is installed.
14. Start the system: a. Toggle the FT Electronics switch to the On position.
The system will boot to Standby mode. Then ion source heater, flow restrictor, and transfer line heaters will start heating.
Monitor these temperatures in the Status View on the right side of the Tune Plus window. (See
.) They will have green check marks when they have reached their operating temperatures.
b. Select the Reagent Ion Source On check box in the Reagent Ion
Source dialog box when the ion source heater, flow restrictor, and transfer line heaters are at their operating temperatures. (See
.)
The Orbitrap Elite ETD mass spectrometer is now ready for use.
Changing the Reagent Ion Source Flow Restrictors
❖
To change the reagent ion source flow restrictors
1. Shut down completely the instrument according to the procedures in
“Shutting Down the Orbitrap Elite Mass Spectrometer
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
User Maintenance
Maintenance of the ETD Module
Warning
Burn Hazard. The reagent vial heaters can be 108 °C (or set point), the flow restrictor, the transfer line heaters, and the ion source heater can be at 160 °C. These components may be too hot to touch.
Verify that all of these components are safe to touch before handling them.
▲
2. Remove the ETD side access panel according to the instructions in
“Removing the ETD Side Access Panel” on page 3-19 .
Caution
The ETD side access panel is interlocked with the
ETD Module power. When the ETD side access panel is removed, all power to the ETD Module will be turned off.
▲
3. Remove the four screws that hold the reagent inlet cover in place.
Warning
Burn Hazard. The reagent inlet cover may be too hot to touch. Verify that the reagent inlet cover is at or near room temperature before handling it.
▲
4. Remove the five screws that hold the restrictor oven cover in place.
Warning
Burn Hazard. The restrictor oven cover may be too hot to touch. Verify that the restrictor oven cover is at or near room temperature before handling it.
▲
5. Replace both fused silica restrictors and their ferrules by removing
their Swagelok® nuts. See Figure 3-48 .
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User Maintenance
Maintenance of the ETD Module
6 5 4 3 2
7
8
9 10 1
Labeled components: 1=PEEKsil
®
tubing, 2=fused silica tubing from lower oven, 3=transfer line inlet, 4=Swagelok fitting with two hole ferrule, 5=fused silica tubing from upper oven, 6=upper oven Swagelok fitting with a single hole ferrule, 7=vial 1 (upper) and vial 2 (lower) heaters, 8=lower oven
Swagelok fitting with a single hole ferrule, 9=Tee below reagent inlet assembly, 10=gas valves
Figure 3-48. Reagent inlet assembly
6. Thread two pieces of fused silica tubing (P/N 98000-20060) into the two hole ferrule (P/N 00101-08-00006) from the Installation
Kit for the Reagent Inlet Module (P/N 98000-62006). Place a
Swagelok fitting over the ferrule.
7. Insert the two hole ferrule and Swagelok fitting from step 6
onto the
transfer line inlet (item 3 in Figure 3-48
) and tighten the Swagelok fitting.
8. Thread the opposite end of one of the pieces of fused silica tubing into a single hole ferrule. Place a Swagelok fitting over the ferrule.
9. Insert the ferrule and Swagelok fitting from step 8 on to one of the
oven outlets (items 6 or 8 in Figure 3-48
) and tighten the Swagelok fitting.
Caution
Do not overtighten the ferrules. The ferrules may loosen after they are first heated. If this occurs retighten them if necessary.
▲
10. Repeat step 9 for the other oven.
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User Maintenance
Maintenance of the ETD Module
11. Replace the restrictor oven cover and reagent inlet cover removed in step 3 and step 4 .
12. Loosen the Swagelok fitting connecting the PEEKsil® tubing (item 1 in
) to the Tee below the reagent inlet assembly (item 9 in
Figure 3-48 ) and from the gas valves (item 10 in Figure 3-48 ).
13. Replace the old PEEKsil tubing with new PEEKsil tubing
(P/N 00109-02-00020) from the Installation Kit for the Reagent
Inlet Module (P/N 98000-62006).
14. Close the ETD Module and restart the instrument. Follow the
procedures given in “Starting Up the System after a Shutdown” on page 2-9
.
Cleaning the Fan Filters of the ETD Module
You need to clean the fan filters every four months. The fan filters are located at the rear of the ETD Module on the left side (as viewed from the back of the ETD Module). See
.
Thermo Fisher Scientific
Fan filters
Figure 3-49. ETD Module, top panel
❖
To clean the fan filters of the ETD Module
1. Remove the fan filter from the rear of the ETD Module by pulling it out of the fan filter bracket.
2. Wash the fan filters in a solution of soap and water.
3. Rinse the fan filters with tap water.
4. Squeeze the water from the fan filters and allow them to air dry.
5. Reinstall the fan filter in the fan filter bracket.
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
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User Maintenance
Maintenance of the Cooling Circuit
Maintenance of the Cooling Circuit
The recirculating chiller and the water filter require maintenance on a regular basis.
Maintenance for the Recirculating Chiller
For the NESLAB ThermoFlex™ 900 recirculating chiller, the checks described in this section should be carried out on a regular basis.
Note For further information and maintenance instructions, refer to the manufacturer’s manual supplied with the instrument.
▲
Reservoir
Periodically inspect the fluid inside the reservoir. If cleaning is necessary, flush the reservoir with a cleaning fluid compatible with the circulating system and the cooling fluid.
The cooling fluid should be replaced periodically. Replacement frequency depends on the operating environment and amount of usage.
Warning
Burn Hazard. Before changing the operating fluid, make sure it is at safe handling temperature.
▲
Fluid Bag Filter
The ThermoFlex 900 recirculating chiller installed in the cooling circuit of the instrument is equipped with a fluid bag filter, which needs to be replaced on a regular basis. Replacement bags are available from Thermo
Fisher Scientific.
Condenser Filter
To prevent a loss of cooling capacity and a premature failure of the cooling system, clean the condenser filter regularly. If necessary, replace it.
Replacing the Water Filter Cartridge
The filter removes particulate matter in the cooling system that might damage the flow sensor. The filter cartridge should be replaced annually or as necessary. A replacement is available from Thermo Fisher Scientific
(P/N 1284050).
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
User Maintenance
Maintenance of the Cooling Circuit
❖
To replace the water filter cartridge
1. Place the system in Standby condition as described on page 2-4
.
2. Turn off the chiller.
3. The filter is installed on the left instrument side, in the cooling lines of the Orbitrap system between the Peltier element and the flow sensor. See
Figure 3-50 . Quick couplings connect the filter assembly
to the hoses of the cooling lines. Press the thumb latch of each quick coupling to release it; valves in the couplings prevent the water from leaking out. Then remove the complete filter assembly.
Thumb latch
Thumb latch
Figure 3-50. Installed water filter
4. Remove the filter from the assembly by using a 5/16 inch wrench and pressing against the gray ring on the quick coupling, away from the filter. See
.
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User Maintenance
Maintenance of the Cooling Circuit
Gray ring
Figure 3-51. Removing the filter cartridge
5. Insert the new filter into the quick couplings. See
Figure 3-52. Filter cartridge with Quick couplers
Caution
Pay special attention to the direction of flow. Reversing the flow can damage both the flow sensor and the filter.
▲
6. Connect the filter assembly to the hoses of the cooling lines.
7. Switch on the chiller system. Check for leaks and check the water level in the chiller. Refill as appropriate.
8. Set the instrument to operating condition.
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Chapter 4
Replaceable Parts
This chapter contains part numbers for replaceable and consumable parts for the mass spectrometer, data system, and kits. To ensure proper results in servicing the Orbitrap Elite system, order only the parts listed or their equivalent.
Note Not all parts are available for purchase separately. Some parts may only be available for purchase as part of a kit or assembly.
▲
For information on how to order parts, see
in the front section of this guide.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
4-1
Replaceable Parts
Ion Sources
Ion Sources
ESI probe, for Ion Max source. . . . . . . . . . . . . . . . . . . . . . . . OPTON-20011
Low flow metal needle for API 2 probes . . . . . . . . . . . . . . . . OPTON-30004
Nanospray II Ion Source . . . . . . . . . . . . . . . . . . . . . . . . . . . . OPTON-20050
Static Nanospray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . OPTON-20051
Dynamic Nanospray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . OPTON-97017
APCI probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . OPTON-20012
APPI probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . OPTON-20026
HESI-II Probe Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . OPTON 20037
High-flow needle insert assembly. . . . . . . . . . . . . . . . . . . . . . OPTON-53010
Low-flow needle insert assembly . . . . . . . . . . . . . . . . . . . . . . OPTON-53011
4-2
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Replaceable Parts
Parts for the Basic System
Parts for the Basic System
Orbitrap Analyzer Installation Kit
Tube, 1/8” × 2.1- 1.4301 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0261000
Ferrule, stainless steel; R. 1/8” . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0520950
Ferrule, stainless steel; V. 1/8” . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0522520
Cap nut, stainless steel; 1/8“. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0520890
Fitting KJH06-00. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1221620
Pumping System
For a schematical overview of the pumping system, see Figure 1-23 on page 1-30
.
T-piece 13 mm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0512360
Hose 13 × 3.5; PVC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0690720
Turbomolecular pump HiPace 300. . . . . . . . . . . . . . . . . . . . . . . . . . .1272910
Turbomolecular pump; HiPace 80 . . . . . . . . . . . . . . . . . . . . . . . . . . .1272920
UHV gauge IKR 270; short . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1181380
Compact Pirani Gauge TPR280. . . . . . . . . . . . . . . . . . . . . . . . . . . . .1156400
Water cooling for HiPace 300. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1272930
Water cooling for HiPace 80. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0794742
PVC hose, with steel helix; ID=45 mm, L=1.6 m . . . . . . . . . . . . . . . .1184330
Hose nipple, DN 40, ISO-KF-45. . . . . . . . . . . . . . . . . . . . . . . . . . . .1159230
Venting flange; DN 10, KF-G1/8” . . . . . . . . . . . . . . . . . . . . . . . . . . .1184400
Splinter shield for TMPs, with DN 100 CF-F flange . . . . . . . . . . . . .1198590
Centering ring, with integrated splinter shield; DN 63 ISO . . . . . . . .1198600
Anti-magnetic cover for IKR 270 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1181390
Gasket; NW 100 CF, copper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0552440
Hose, metal; KF16-KF25 - 250mm . . . . . . . . . . . . . . . . . . . . . . . . . .1154130
Gasket; copper, NW 35 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0550480
Metal tube, KF NW16×250 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0524260
KF Tee piece; NW 16 KF, stainless steel . . . . . . . . . . . . . . . . . . . . . . .0524230
Centering ring with o-ring; DN 16, Viton . . . . . . . . . . . . . . . . . . . . .0522140
Centering ring with o-ring; DN 25, Viton . . . . . . . . . . . . . . . . . . . . .0522150
Centering ring; NW 16/10, aluminum-Viton . . . . . . . . . . . . . . . . . .0522200
Clamping ring; NW 10/16, KF . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0521830
Clamping ring; NW 20/25, KF . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0521560
Reducing cross piece; DN40/DN16 KF . . . . . . . . . . . . . . . . . . . . . . .1184310
Metal tube; DN40x500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1184350
Metal tube; DN40x750 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1181290
Hose clamp; NW 40 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1181320
Centering ring; NW 40 KF, aluminum-Viton . . . . . . . . . . . . . . . . . .0522260
Tension ring; NW 32/40 KF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1181250
Clamping screw; DN63-100 ISO, aluminum. . . . . . . . . . . . . . . . . . .1042670
Flexible metal hose KF NW 16x500. . . . . . . . . . . . . . . . . . . . . . . . . .0534500
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
4-3
Replaceable Parts
Parts for the Basic System
Water Supply
For a schematical overview of the cooling water circuit, see Figure 1-32 on page 1-42
.
Quick coupling insert; 9.6 mm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1141640
Quick coupling body; 9.6 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1138960
Hose; 9 x 3, black, PVC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1049540
Hose; 6 x 1, Teflon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1042660
Quick coupling insert; Delrin Acetal, NW 6.4 . . . . . . . . . . . . . . . . . 1185030
Quick coupling body; Delrin Acetal, NW 6.4 . . . . . . . . . . . . . . . . . . 1185020
Clamping piece 8/16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0370130
Adaptor hose nipple; male, 1/2 x 10 . . . . . . . . . . . . . . . . . . . . . . . . . 1185840
Flow control sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1191740
Filter cartridge; 50 μm DIF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1284050
Gas Supply
For a schematical overview of the gas supply, see
Bulkhead union; 1/16”, for hose 4 x 1 (for P/N 069 1130) . . . . . . . . 1153660
Bulkhead union; 1/8”×1/8” . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0523450
Hose; 4 x 1, Teflon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0690280
Hose; 4 x 1, polyurethane, blue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0691130
Capillary 1/16” ID-SS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0605470
Plug-in T-piece; 3 x 6mm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1128140
Regulator + manometer f. Orbitrap analyzer . . . . . . . . . . . . . . . . . . . 1257670
Capillary; 1/16”x0.13x400mm (red), PEEK . . . . . . . . . . . . . . . . . . . 1253830
Coupling; 1/16”, SS-100-6 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0524340
Ferrule; 1/16” GVF 16-000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1121110
Reducer Swagelok; 1/8” × 1/16”, stainless steel . . . . . . . . . . . . . . . . . 0662880
Ferrule; 1/16” GVF/16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0674800
Connector 1/8”, for hose OD 4 mm . . . . . . . . . . . . . . . . . . . . . . . . . 1128680
Cap nut; 1/16”, stainless steel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0520880
Hose; 2 x 1, PTFE. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1091650
Sleeve; Ø 6 mm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1047320
Capillary; PEEKsil, 1/16”, 0.1 × 500 mm . . . . . . . . . . . . . . . . . . . . . 1223420
Plug, KQ2P-06 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1185620
Cap, KQ2C-06 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1258220
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Replaceable Parts
Parts Lists for the ETD System
Parts Lists for the ETD System
This section contains parts lists for the components of the ETD System of the Orbitrap Elite ETD mass spectrometer.
Quadrupole Orbitrap analyzer, complete . . . . . . . . . . . . . . . . . . . . . .1239200
Housing HCD/ETD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1231740
Separating plate HCD/ETD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1231770
Screw M 4 x 8 DIN912 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1044420
O-ring 129 X 4 A Viton. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1240520
Lid HCD/ETD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1231760
Screw-in connector; 1/16” . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1186150
Gasket; NW 63 ISO, aluminum/Viton . . . . . . . . . . . . . . . . . . . . . . .0554060
Blank flange; stainless steel, NW 63 . . . . . . . . . . . . . . . . . . . . . . . . . .0652620
Clamping screw; DN63-100, aluminum . . . . . . . . . . . . . . . . . . . . . .1028380
Washer 8.4; stainless steel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0470070
Screw, hexagonal; M 8 x 35, stainless steel . . . . . . . . . . . . . . . . . . . . .0454400
Washer 8.4 x 11 x 1.5, stainless steel. . . . . . . . . . . . . . . . . . . . . . . . . .0470860
Screw M8 x 35; stainless steel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0454250
Centering ring NW 16 Viton. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0522140
Feedthrough; 8-fold 1,5kV DN16KF. . . . . . . . . . . . . . . . . . . . . . . . .1231750
O-ring; 118 X 5 A, Viton. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1168240
Box f. feedthrough; KF16 / Sub-D9 . . . . . . . . . . . . . . . . . . . . . . . . . .1231800
Cylindrical Screw ISO4762-M6X12-A4. . . . . . . . . . . . . . . . . . . . . . .0453300
Vacuum system ETD
Flange clamp; KF16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1145860
PUMP, TURBO, EDWARDS EXT75DX ISO100, TNR . . 00108-01-00016
Splinter guard, DN_63_ISO . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1198600
Metal hose, DN 16 ISO-KF x500 . . . . . . . . . . . . . . . . . . . . . . . . . . .1181410
Centering ring with o-ring; DN 16, Viton . . . . . . . . . . . . . . . . . . . . .0522140
Clamping ring; NW 10/16, KF . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0521830
Hose flange; NW 25 KF. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1042330
Centering ring, with o ring; DN 25, Viton . . . . . . . . . . . . . . . . . . . .0522150
Clamping ring, DN 25. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0521560
Flange, KF16 - hose OD 19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1239340
Reducer; DN 16/DN 25, aluminum . . . . . . . . . . . . . . . . . . . . . . . . .0522160
PUMP, ROTARY VANE, EDWARDS RV3. . . . . . . . . . . . . . 00108-01-0008
KIT, ACCESSORY, MECHANICAL PUMP, RV3 . . . . . . . . . 98000-620007
Gas Supply ETD
Plug-in T-piece; 3 x 6mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1128140
Sleeve 6 mm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1047320
Reducing hose connector, 3.2−>6. . . . . . . . . . . . . . . . . . . . . . . . . . .1239220
Hose cutter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1239280
T-piece 13 mm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0512360
Hose 9 X 3; PVC, black . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1049540
Clamping piece 8/16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0370130
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
4-5
Replaceable Parts
Parts Lists for the ETD System
Hose; 4 X 1, Teflon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0690280
Clamping ring; stainless steel, NW10/16. . . . . . . . . . . . . . . . . . . . . . 1149200
PEEK capillary; 1/16” x 0.040. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1245940
Ferrule, 1/16”, for GVF/16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0674800
Ferrule 1/16” - CTFE, collapsible . . . . . . . . . . . . . . . . . . . . . . . . . . . 1224700
Electronic Parts ETD
Cable Y-ADAPTER/T.PUMP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2108630
Coupling; RJ45 BU/2BU . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2075210
Patch cable; 0.51MT RJ45 gray SFTP. . . . . . . . . . . . . . . . . . . . . . . . 2080870
Cable POWER DIS./T-PUMP . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2081200
Cable CLT-OFFSET-A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2108710
Cable IOS ETD/ION OPTIC-S . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2108820
UNIT_ION OPTIC SUPPLY ETD . . . . . . . . . . . . . . . . . . . . . . . . . 2108920
PCB LTQ CABLE DRIVER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2097780
PCB ORBITRAP CABLE RECEIVER. . . . . . . . . . . . . . . . . . . . . . 2097830
PCB ETD CABLE RECEIVER . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2097800
Cable ETD/LTQ/ORBITRAP-INTERCONNECT 60 . . . . . . . . . . 2108940
Cable ETD/LTQ/ORBITRAP-INTERCONNECT 36 . . . . . . . . . . 2108950
Cable ETD/LTQ/ORBITRAP-INTERCON SUPPLY . . . . . . . . . . 2108960
Coaxial cable; ETD IOS/ANALOG CTRL, J5554 . . . . . . . . . . . . . 2108990
Coaxial cable; ETD IOS/ANALOG CTRL, J5555 . . . . . . . . . . . . . 2109000
Coaxial cable; ETD IOS/ANALOG CTRL, J5556 . . . . . . . . . . . . . 2109010
Cable ANALOG CTRL / ETD IOS . . . . . . . . . . . . . . . . . . . . . . . . 2108970
Cable ETD IOS/HCD multipole . . . . . . . . . . . . . . . . . . . . . . . . . . . 2108980
Extension cable; 16A C20-C19 2M . . . . . . . . . . . . . . . . . . . . . . . . . 2097050
ADAPTER_IOS/MULTIPOLE-HCD . . . . . . . . . . . . . . . . . . . . . . 2100410
Reagent Inlet Module
ASSY, TOOL, ION VOLUME INSERTION/REMOVAL . . . 98000-60028
TUBING, PEEKsil, 1/16” OD, 100mm LONG, RoHS. . . .00109-02-00020
FRLE, 1HOLE, 1/16 OD, 0.4mm ID, VESP/GRPHT, RO .00101-08-00005
FRLE, 2HOLS, 1/16 OD, 0.4mm ID, VESP/GRPHT, RO .00101-08-00006
Inlet Valve Seal Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119265-0003
Inlet valve seal removal tool. . . . . . . . . . . . . . . . . . . . . . . . . 119283-0001
Spool inlet valve seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119683-0100
O-ring Viton, 0530 ID × 0.082 W . . . . . . . . . . . . . . . . . . . . . 3814-6530
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
ETD Reagent Kit
Replaceable Parts
Parts Lists for the ETD System
ETD Reagent Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .98000-62008
Angiotensin I, 1mg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 00301-15517
Fluoranthene, 150mg . . . . . . . . . . . . . . . . . . . . . . . . . . . 00301-01-0013
The fluoranthene in your ETD Reagent Kit is Sigma/Aldrich Supelco
#48535. The fluoranthene MSDS is obtained from the MSDS link at: www.sigmaaldrich.com/catalog/search/ProductDetail/SUPELCO/48535
Thermo Fisher Scientific supplies fluoranthene as a two vial kit. One vial contains 150 mg of fluoranthene and the other is the required empty vial.
The angiotensin I in your ETD Reagent Kit is Angiotensin I human acetate hydrate (Sigma/Aldrich #A9650). Angiotensin I is potentially hazardous. Handle it in accordance with its MSDS. The angiotensin I
MSDS is obtained from the MSDS link at: www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A9650
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
4-7
Appendix A
Fluoranthene
Fluoranthene is used as the Electron Transfer Dissociation (ETD) reagent in the ETD Module portion of the Orbitrap Elite ETD mass spectrometer. The fluoranthene radical anion is generated according to
the reaction shown in Figure A-1.
Thermo Fisher Scientific
Fluoranthene
Figure A-1. ETD Reagent (fluoranthene radical anion) generation from fluoranthene
Fluoranthene is potentially hazardous. Use it in accordance with its
Material Safety Data Sheet (MSDS).
Note Store and handle all chemicals in accordance with standard safety procedures. The MSDS describing the chemicals being used should be freely available to lab personnel for them to examine at any time.
Material Safety Data Sheets (MSDSs) provide summarized information on the hazard and toxicity of specific chemical compounds. The MSDS also provides information on the proper handling of compounds, first aid for accidental exposure, and procedures for cleaning spills or dealing with leaks. Producers and suppliers of chemical compounds are required by law to provide their customers with the most current health and safety information in the form of an MSDS. Read the MSDS for each chemical you use. Dispose of all laboratory reagents in the appropriate way (see the MSDS).
▲
The fluoranthene contained in the ETD Reagent Kit
(P/N 98000-62008, see
page 4-7 ) is Sigma/Aldrich Supelco #48535.
The fluoranthene MSDS is obtained from the MSDS link at: www.sigmaaldrich.com/catalog/search/ProductDetail/SUPELCO/48535
Thermo Fisher Scientific supplies fluoranthene as a two vial kit. One vial contains 150 mg of fluoranthene and the other is the required empty vial.
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
A-1
Glossary
This section lists and defines terms used in this guide. It also includes acronyms, metric prefixes, symbols, and abbreviations.
J
Y Z
A
A ampere
AC alternating current
ADC analog-to-digital converter; a device that converts data from analog to digital form.
adduct ion An ion formed by the joining together of two species, usually an ion and a molecule, and often within the ion source, to form an ion containing all the constituent atoms of both species.
AGC™ See
.
APCI See atmospheric pressure chemical ionization
APCI corona discharge current The ion current carried by the charged particles in the APCI source.
The voltage on the APCI corona discharge needle supplies the potential required to ionize the particles.
The APCI corona discharge current is set; the APCI corona discharge voltage varies, as required, to maintain the set discharge current.
APCI corona discharge voltage .
APCI corona discharge voltage The high voltage that is applied to the corona discharge needle in the APCI source to produce the APCI corona discharge. The corona discharge voltage varies, as required, to maintain the set APCI spray current.
See also APCI spray current .
APCI manifold The manifold that houses the APCI sample tube and nozzle, and contains the plumbing for the sheath and auxiliary gas.
APCI needle, corona discharge A needle to which a sufficiently high voltage (typically ±3 to ±5 kV) is applied to produce a chemical ionization plasma by the corona discharge mechanism.
See also chemical ionization (CI) , chemical ionization
atmospheric pressure chemical ionization
.
APCI nozzle The nozzle in the APCI probe that sprays the sample solution into a fine mist.
See also atmospheric pressure chemical ionization
APCI sample tube A fused silica tube that delivers sample solution to the APCI nozzle . The APCI sample tube extends from the sample inlet to the
APCI nozzle.
See also atmospheric pressure chemical ionization
API stack .
APCI source Contains the APCI probe assembly,
APCI manifold, and API stack.
See also atmospheric pressure chemical ionization
APCI manifold , and API stack .
APCI spray current The ion current carried by the charged particles in the APCI source. The APCI corona discharge voltage varies, as required, to maintain the set spray current.
APCI vaporizer A heated tube that vaporizes the sample solution as the solution exits the sample tube and enters the atmospheric pressure region of the
APCI source.
See also atmospheric pressure chemical ionization
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
G-1
Glossary: API–atmospheric pressure chemical ionization (APCI)
API See atmospheric pressure ionization (API)
.
API atmospheric pressure region The first of two chambers in the API source. Also referred to as the spray chamber.
API capillary-skimmer region The area between the capillary and the skimmer, which is surrounded by the tube lens. It is also the area of first-stage evacuation in the API source.
API heated capillary A tube assembly that assists in desolvating ions that are produced by the ESI or
APCI probe.
See also API heated capillary voltage .
API heated capillary voltage The DC voltage applied to the heated capillary. The voltage is positive for positive ions and negative for negative ions.
See also API source and API heated capillary .
API ion transfer capillary A tube assembly that assists in desolvating ions that are produced by the ESI, NSI, or APCI probe.
See also API ion transfer capillary offset voltage and
API ion transfer capillary temperature .
API ion transfer capillary offset voltage A DC voltage applied to the ion transfer capillary. The voltage is positive for positive ions and negative for negative ions.
See also API source and API ion transfer capillary .
API ion transfer capillary temperature The temperature of the ion transfer capillary, which should be adjusted for different flow rates.
See also API source and API ion transfer capillary .
API source The sample interface between the LC and the mass spectrometer. It consists of the API probe
(ESI or APCI) and API stack.
See also
atmospheric pressure ionization (API) ,
ESI source , APCI source , ESI probe , and API stack .
API spray chamber The first of two chambers in the
API source. In this chamber the sample liquid exits the probe and is sprayed into a fine mist (ESI or NSI) or is vaporized (APCI) as it is transported to the entrance end of the ion transfer capillary.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
API spray shield A stainless steel, cylindrical vessel that, in combination with the ESI or APCI flange, forms the atmospheric pressure region of the
API source.
See also atmospheric pressure ionization (API) .
API stack Consists of the components of the
API source that are held under vacuum and includes the API spray shield , API ion transfer capillary ,
API tube lens ,
, the ion transfer capillary mount, and the tube lens and skimmer mount.
See also atmospheric pressure ionization (API)
and
API source .
API tube lens A lens in the API source that separates ions from neutral particles as they leave the ion transfer capillary. A potential applied to the tube lens focuses the ions toward the opening of the skimmer and helps to dissociate adduct ions.
See also API tube lens offset voltage , API source ,
API ion transfer capillary , and
.
API tube lens and skimmer mount A mount that attaches to the heated capillary mount. The tube lens and skimmer attach to the tube lens and skimmer mount.
API tube lens offset voltage A DC voltage applied to the tube lens. The value is normally tuned for a specific compound.
See also API tube lens ,
AP-MALDI See atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) .
APPI See
Atmospheric Pressure Photoionization
.
ASCII American Standard Code for Information
Interchange
atmospheric pressure chemical ionization (APCI) A soft ionization technique done in an ion source operating at atmospheric pressure. Electrons from a corona discharge initiate the process by ionizing the mobile phase vapor molecules. A reagent gas forms, which efficiently produces positive and negative ions of the analyte through a complex series of chemical reactions.
See also electrospray ionization (ESI) .
Thermo Fisher Scientific
Glossary: atmospheric pressure ionization (API)–consecutive reaction monitoring (CRM) scan type
atmospheric pressure ionization (API) Ionization performed at atmospheric pressure by using
atmospheric pressure chemical ionization (APCI)
,
, or
atmospheric pressure matrix-assisted laser
desorption/ionization (AP-MALDI) Matrix-assisted laser desorption/ionization in which the sample target is at atmospheric pressure.
See also matrix-assisted laser desorption/ionization
.
Atmospheric Pressure Photoionization (APPI) A soft ionization technique in which an ion is generated from a molecule when it interacts with a photon from a light source.
atomic mass unit Atomic Mass Unit (u) defined by taking the mass of one atom of carbon12 as being
12u; unit of mass for expressing masses of atoms or molecules.
Automatic Gain Control™ (AGC) Sets the ion injection time to maintain the optimum quantity of ions for each scan. With AGC on, the scan function consists of a prescan and an analytical scan.
.
auxiliary gas The outer-coaxial gas (nitrogen) that assists the sheath (inner-coaxial) gas in dispersing and/or evaporating sample solution as the sample solution exits the APCI, ESI, or H-ESI nozzle.
auxiliary gas flow rate The relative rate of flow of
auxiliary gas (nitrogen) into the API source reported
in arbitrary units.
auxiliary gas inlet An inlet in the API probe where auxiliary gas is introduced into the probe.
See also auxiliary gas and atmospheric pressure ionization (API)
.
auxiliary gas plumbing The gas plumbing that delivers outer coaxial nitrogen gas to the ESI or APCI nozzle.
auxiliary gas valve A valve that controls the flow of auxiliary gas into the API source.
B
b bit
B byte (8 b)
baud rate data transmission speed in events per second
BTU British thermal unit, a unit of energy
C
°C degrees Celsius
CE central electrode (of the Orbitrap analyzer);
European conformity. Mandatory European marking for certain product groups to indicate conformity with essential health and safety requirements set out in
European Directives.
cfm cubic feet per minute
chemical ionization (CI) The formation of new ionized species when gaseous molecules interact with ions. The process can involve transfer of an electron, proton, or other charged species between the reactants.
chemical ionization (CI) plasma The collection of ions, electrons, and neutral species formed in the ion source during chemical ionization.
See also chemical ionization (CI) .
CI See
CID See
collision-induced dissociation (CID) .
cm centimeter
cm
3
cubic centimeter
collision gas A neutral gas used to undergo collisions with ions.
collision-induced dissociation (CID) An ion/neutral process in which an ion is dissociated as a result of interaction with a neutral target species.
consecutive reaction monitoring (CRM) scan type A scan type with three or more stages of mass analysis and in which a particular multi-step reaction path is monitored.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
G-3
Glossary: Convectron™ gauge–ESI flange
Convectron™ gauge A thermocouple bridge gauge that is sensitive to the pressure as well as the thermal conductivity of the gas used to measure pressures between X and Y.
corona discharge In the APCI source, an electrical discharge in the region around the corona discharge needle that ionizes gas molecules to form a chemical ionization (CI) plasma, which contains CI reagent ions.
See also
chemical ionization (CI) plasma and
atmospheric pressure chemical ionization (APCI) .
CPU central processing unit (of a computer)
CRM See consecutive reaction monitoring (CRM) scan type .
C-Trap curved linear trap
<Ctrl> control key on the terminal keyboard
D
d depth
Da dalton
DAC digital-to-analog converter
damping gas Helium gas introduced into the ion trap mass analyzer that slows the motion of ions entering the mass analyzer so that the ions can be trapped by the RF voltage fields in the mass analyzer.
data-dependent scan A scan mode that uses specified criteria to select one or more ions of interest on which to perform subsequent scans, such as MS/MS or
ZoomScan.
DC direct current
divert/inject valve A valve on the mass spectrometer that can be plumbed as a divert valve or as a loop injector.
DS data system
DSP digital signal processor
E
ECD See electron capture dissociation (ECD)
.
EI electron ionization
electron capture dissociation (ECD) A method of fragmenting gas phase ions for tandem mass spectrometric analysis. ECD involves the direct introduction of low energy electrons to trapped gas phase ions.
See also electron transfer dissociation (ETD)
and
infrared multiphoton dissociation (IRMPD)
.
electron multiplier A device used for current amplification through the secondary emission of electrons. Electron multipliers can have a discrete dynode or a continuous dynode.
electron transfer dissociation (ETD) A method of fragmenting peptides and proteins. In electron transfer dissociation (ETD), singly charged reagent anions transfer an electron to multiply protonated peptides within the ion trap mass analyzer. This leads to a rich ladder of sequence ions derived from cleavage at the amide groups along the peptide backbone.
Amino acid side chains and important modifications such as phosphorylation are left intact.
electrospray ionization (ESI) A type of atmospheric pressure ionization that is currently the softest ionization technique available to transform ions in solution into ions in the gas phase.
EMBL European Molecular Biology Laboratory
<Enter> Enter key on the terminal keyboard
ESD ElectroStatic Discharge. Discharge of stored static electricity that can damage electronic equipment and impair electrical circuitry, resulting in complete or intermittent failures.
ESI See
.
ESI flange A flange that holds the ESI probe in position next to the entrance of the heated capillary, which is part of the API stack. The ESI flange also seals the atmospheric pressure region of the API source and, when it is in the engaged position against the spray shield, compresses the high-voltage safety-interlock switch.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
ESI probe A probe that produces charged aerosol droplets that contain sample ions. The ESI probe is typically operated at liquid flows of 1 μL/min to
1 mL/min without splitting. The ESI probe includes the ESI manifold, sample tube, nozzle, and needle.
ESI source Contains the ESI probe and the API stack.
See also electrospray ionization (ESI)
, ESI probe , and
API stack .
ESI spray current The flow of charged particles in the
ESI source. The voltage on the ESI spray needle supplies the potential required to ionize the particles.
ESI spray voltage The high voltage that is applied to the spray needle in the ESI source to produce the
ESI spray current. In ESI, the voltage is applied to the spray liquid as it emerges from the nozzle.
See also ESI spray current .
ETD See
electron transfer dissociation (ETD) .
eV Electron Volt. The energy gained by an electron that accelerates through a potential difference of one volt.
Extensible Markup Language See XML (Extensible
external lock mass A lock that is analyzed in a separate
MS experiment from your sample. If you need to run a large number of samples, or if accurate mass samples will be intermingled with standard samples, you might want to use external lock masses. These allow more rapid data acquisition by eliminating the need to scan lock masses during each scan.
F
f femto (10
-15)
°F degrees Fahrenheit
.fasta file extension of a SEQUEST™ search database file
ft foot
Glossary: ESI probe–FWHM
Fast Fourier Transform (FFT) An algorithm that performs a Fourier transformation on data. A Fourier transform is the set of mathematical formulae by which a time function is converted into a frequency-domain function and the converse.
FFT See
.
fluoranthene A reagent anion that is used in an
electron transfer dissociation (ETD)
experiment.
firmware Software routines stored in read-only memory. Startup routines and low-level input/output instructions are stored in firmware.
forepump The pump that evacuates the foreline. A rotary-vane pump is a type of forepump.
Fourier Transform - Ion Cyclotron Resonance Mass
Spectrometry (FT-ICR MS) A technique that determines the mass-to-charge ratio of an ion by measuring its cyclotron frequency in a strong magnetic field.
fragment ion A charged dissociation product of an ionic fragmentation. Such an ion can dissociate further to form other charged molecular or atomic species of successively lower formula weights.
fragmentation The dissociation of a molecule or ion to form fragments, either ionic or neutral. When a molecule or ion interacts with a particle (electron, ion, or neutral species) the molecule or ion absorbs energy and can subsequently fall apart into a series of charged or neutral fragments. The mass spectrum of the fragment ions is unique for the molecule or ion.
FT Fourier Transformation
FT-ICR MS See Fourier Transform - Ion Cyclotron
Resonance Mass Spectrometry (FT-ICR MS) .
FTMS Fourier Transformation Mass Spectroscopy
full-scan type Provides a full mass spectrum of each analyte or parent ion. With the full-scan type, the mass analyzer is scanned from the first mass to the last mass without interruption. Also known as single-stage full-scan type.
FWHM Full Width at Half Maximum
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
G-5
Glossary: g–ion optics
G
g gram
G Gauss; giga (10
9
)
GC gas chromatograph; gas chromatography
GC/MS gas chromatography / mass spectrometer
GUI graphical user interface
H
h hour
h height
handshake A signal that acknowledges that communication can take place.
HCD See
higher energy collision-induced dissociation
header information Data stored in each data file that summarizes the information contained in the file.
H-ESI probe Heated-electrospray ionization (H-ESI) converts ions in solution into ions in the gas phase by using
electrospray ionization (ESI) in combination
higher energy collision-induced dissociation (HCD)
Collision-induced dissociation that occurs in the
HCD cell of the
. The
HCD cell consists of a straight multipole mounted inside a collision gas-filled tube. A voltage offset between C-Trap and HCD cell accelerates parent ions into the collision gas inside the HCD cell, which causes the ions to fragment into product ions. The product ions are then returned to the Orbitrap analyzer for mass analysis. HCD produces triple quadrupole-like product ion mass spectra.
high performance liquid chromatography (HPLC)
Liquid chromatography in which the liquid is driven through the column at high pressure. Also known as high pressure liquid chromatography.
HPLC See
high performance liquid chromatography
HV high voltage
G-6
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
Hz hertz (cycles per second)
I
ICR ion cyclotron resonance
ID inside diameter
IEC International Electrotechnical Commission
IEEE Institute of Electrical and Electronics Engineers
in. inch
infrared multiphoton dissociation (IRMPD) In infrared multiphoton dissociation (IRMPD), multiply charged ions consecutively absorb photons emitted by a infrared laser until the vibrational excitation is sufficient for their fragmentation. The fragments continue to pick up energy from the laser pulse and fall apart further to ions of lower mass.
See also electron capture dissociation (ECD) .
instrument method A set of experiment parameters that define Xcalibur operating settings for the autosampler, liquid chromatograph (LC), mass spectrometer, divert valve, syringe pump, and so on.
Instrument methods are saved as file type .meth.
internal lock mass A lock that is analyzed during the same MS experiment as your sample and is contained within the sample solution or infused into the
LC flow during the experiment. Internal lock masses provide the most accurate corrections to the data.
I/O input/output
ion gauge Measures the pressure in the mass analyzer region (high vacuum region) of the vacuum manifold.
ion injection time The amount of time that ions are allowed to accumulate in the ion trap mass analyzer when AGC is off. With AGC on, the ion injection time is set automatically (up to the set maximum ion injection time) based on the AGC target value.
See also: Automatic Gain Control™ (AGC) .
ion optics Focuses and transmits ions from the
API source to the mass analyzer.
Thermo Fisher Scientific
ion source A device that converts samples to gas-phase ions.
ion sweep cone A removable cone-shaped metal cover that fits on top of the API ion transfer capillary and acts as a physical barrier to protect the entrance of the capillary.
ion sweep gas Extra nitrogen gas that flows along the axis of the API ion transfer capillary (between the ion sweep cone and the capillary block) towards the API spray. The sweep gas flow is thus countercurrent to the flow of the ions.
See also ion sweep gas pressure .
ion sweep gas pressure The rate of flow of the sweep gas (nitrogen) into the API source. A measurement of the relative flow rate (in arbitrary units) to provide the required flow of nitrogen gas out from the Ion Sweep cone towards the API spray.
.
IRMPD See infrared multiphoton dissociation
K
k kilo (10
3
, 1000)
K kilo (2
10
, 1024)
KEGG Kyoto Encyclopedia of Genes and Genomes
kg kilogram
L
l length
L liter
LAN local area network
lb pound
LC See
.
LC/MS See liquid chromatography / mass spectrometry
.
LED light-emitting diode
LHe liquid helium
Thermo Fisher Scientific
Glossary: ion source–min
liquid chromatography (LC) A form of elution chromatography in which a sample partitions between a stationary phase of large surface area and a liquid mobile phase that percolates over the stationary phase.
liquid chromatography / mass spectrometry
(LC/MS) An analytical technique in which a high-performance liquid chromatograph (LC) and a mass spectrometer (MS) are combined.
LN2 liquid nitrogen
lock mass A known reference mass in the sample that is used to correct the mass spectral data in an accurate mass experiment and used to perform a real-time secondary mass calibration that corrects the masses of other peaks in a scan. Lock masses with well-defined, symmetrical peaks work best. You can choose to use
or
.
log file A text file, with a .log file extension, that is used to store lists of information.
M
μ micro (10 -6
)
m meter; milli (10
-3
)
M mega (10
6
)
M
+
molecular ion
MALDI See matrix-assisted laser desorption/ionization
.
matrix-assisted laser desorption/ionization
(MALDI) A method of ionizing proteins where a direct laser beam is used to facilitate vaporization and ionization while a matrix protects the biomolecule from being destroyed by the laser.
MB Megabyte (1048576 bytes)
MH
+
protonated molecular ion
microscan One mass analysis (ion injection and storage or scan-out of ions) followed by ion detection.
Microscans are summed, to produce one scan, to improve the signal-to-noise ratio of the mass spectral data. The number of microscans per scan is an important factor in determining the overall scan time.
min minute
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
G-7
Glossary: mL–Orbitrap mass analyzer
mL milliliter
mm millimeter
MRFA A peptide with the amino acid sequence methionine–arginine–phenylalanine–alanine.
MS mass spectrometer; mass spectrometry
MS MS n
power: where n = 1
MS scan modes Scan modes in which only one stage of mass analysis is performed. The scan types used with
the MS scan modes are full-scan type
and
selected ion monitoring (SIM) scan type
.
MSDS Material Safety Data Sheet
MS/MS Mass spectrometry/mass spectrometry, or tandem mass spectrometry is an analytical technique that involves two stages of mass analysis. In the first stage, ions formed in the ion source are analyzed by an initial analyzer. In the second stage, the mass-selected ions are fragmented and the resultant ionic fragments are mass analyzed.
MS n
scan mode The scan power equal to 1 to 10, where the scan power is the power n in the expression
MS n
. MS n
is the most general expression for the scan mode, which can include the following:
• The scan mode corresponding to the one stage of mass analysis in a single-stage full-scan experiment or a selected ion monitoring (SIM) experiment
• The scan mode corresponding to the two stages of mass analysis in a two-stage full-scan experiment or a selected reaction monitoring (SRM) experiment
• The scan mode corresponding to the three to ten stages of mass analysis (n = 3 to n = 10) in a multi-stage full-scan experiment or a consecutive reaction monitoring (CRM) experiment
See also
multipole A symmetrical, parallel array of (usually) four, six, or eight cylindrical rods that acts as an ion transmission device. An RF voltage and DC offset voltage are applied to the rods to create an electrostatic field that efficiently transmits ions along the axis of the multipole rods.
m/z Mass-to-charge ratio. An abbreviation used to denote the quantity formed by dividing the mass of an ion (in u) by the number of charges carried by the ion.
For example, for the ion C
7
H
7
2+
, m/z=45.5.
N
n nano (10
-9
)
nanospray ionization (NSI) A type of electrospray ionization (ESI) that accommodates very low flow rates of sample and solvent on the order of 1 to
20 nL/min (for static nanospray) or 100 to 1000 nL/min (for dynamic nanospray).
NCBI National Center for Biotechnology Information
(USA)
NIST National Institute of Standards and Technology
(USA)
NMR Normal Mass Range
NSI See nanospray ionization (NSI) .
O
octapole An octagonal array of cylindrical rods that acts as an ion transmission device. An RF voltage and
DC offset voltage applied to the rods create an electrostatic field that transmits the ions along the axis of the octapole rods.
OD
outside diameter
Orbitrap mass analyzer The Orbitrap™ mass analyzer consists of a spindle-shape central electrode surrounded by a pair of bell-shaped outer electrodes.
Ions inside the mass analyzer orbit in stable trajectories around the central electrode with harmonic oscillations along it.
r z
Two detection electrodes record an image current of the ions as they undergo harmonic oscillations. A
Fourier transformation extracts different harmonic frequencies from the image current. An ion's
G-8
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
mass-to-charge ratio m/z is related to the frequency f of its harmonic oscillations and to the instrumental constant k by:
m/z = k/f
2
OT Orbitrap
See
.
OVC outer vacuum case
Ω ohm
P
p pico (10
-12
)
Pa pascal
parent ion An electrically charged molecular species that can dissociate to form fragments. The fragments can be electrically charged or neutral species. A parent ion can be a molecular ion or an electrically charged fragment of a molecular ion. Also called a precursor ion.
parent mass The mass-to-charge ratio of a parent ion.
The location of the center of a target parent-ion peak in mass-to-charge ratio (m/z) units. Also known as precursor mass.
PCB printed circuit board
PDA detector Photodiode Array detector is a linear array of discrete photodiodes on an integrated circuit chip. It is placed at the image plane of a spectrometer to allow a range of wavelengths to be detected simultaneously.
PE protective earth
PID proportional / integral / differential
P/N part number
p-p peak-to-peak voltage
ppm parts per million
PQD pulsed-Q dissociation
Glossary: OT–retention time (RT)
precursor ion An electrically charged molecular species that can dissociate to form fragments. The fragments can be electrically charged or neutral species. A precursor ion (PR) can be a molecular ion or an electrically charged fragment of a molecular ion. Also known as parent ion.
precursor mass Mass of the corresponding precursor
(or parent) ion or molecule.
psig pounds per square inch, gauge
PTM posttranslational modification
pulsed Q dissociation (PQD) Collision-induced dissociation that involves precursor ion activation at high Q, a time delay to allow the precursor to fragment, and then a rapid pulse to low Q where all fragment ions are trapped. The fragment ions can then be scanned out of the ion trap mass analyzer and detected. PQD eliminates the “1/3 Rule” low mass cut-off for MS/MS data.
Q
quadrupole A symmetrical, parallel array of four hyperbolic rods that acts as a mass analyzer or an ion transmission device. As a mass analyzer, one pair of opposing rods has an oscillating radio frequency (RF) voltage superimposed on a positive direct current
(DC) voltage. The other pair has a negative DC voltage and an RF voltage that is 180 degrees out of phase with the first pair of rods. This creates an electrical field (the quadrupole field) that efficiently transmits ions of selected mass-to-charge ratios along the axis of the quadrupole rods.
R
RAM random access memory
raw data Uncorrected liquid chromatograph and mass spectrometer data obtained during an acquisition.
Xcalibur and Xcalibur-based software store this data in a file that has a .raw file extension.
resolution The ability to distinguish between two points on the wavelength or mass axis.
retention time (RT) The time after injection at which a compound elutes. The total time that the compound is retained on the chromatograph column.
Thermo Fisher Scientific Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
G-9
Glossary: RF–SIM
RF radio frequency
RF lens A multipole rod assembly that is operated with only radio frequency (RF) voltage on the rods. In this type of device, virtually all ions have stable trajectories and pass through the assembly.
RF voltage An AC voltage of constant frequency and variable amplitude that is applied to the ring electrode or endcaps of the mass analyzer or to the rods of a multipole. Because the frequency of this AC voltage is in the radio frequency (RF) range, it is referred to as
RF voltage.
RMS root mean square
ROM read-only memory
rotary-vane pump A mechanical vacuum pump that establishes the vacuum necessary for the proper operation of the turbomolecular pump. (Also called a roughing pump or forepump.)
RS-232 An accepted industry standard for serial communication connections. This Recommended
Standard (RS) defines the specific lines and signal characteristics used by serial communications controllers to standardize the transmission of serial data between devices.
RT An abbreviated form of the phrase
retention time
(RT)
. This shortened form is used to save space when the retention time (in minutes) is displayed in a header, for example, RT: 0.00-3.75.
S
s second
scan mode and scan type combinations A function that coordinates the three processes in the
MS detector: ionization, mass analysis, and ion detection. You can combine the various scan modes and scan types to perform a wide variety of experiments.
selected ion monitoring (SIM) scan type A scan type in which the mass spectrometer acquires and records ion current at only one or a few selected mass-to-charge ratio values.
See also selected reaction monitoring (SRM) scan type .
selected reaction monitoring (SRM) scan type A scan type with two stages of mass analysis and in which a particular reaction or set of reactions, such as the fragmentation of an ion or the loss of a neutral moiety, is monitored. In SRM a limited number of product ions is monitored.
SEM secondary electron multiplier
Serial Peripheral Interface (SPI) hardware and firmware communications protocol
serial port An input/output location (channel) for serial data transmission.
sheath gas The inner coaxial gas (nitrogen), which is used in the API source to help nebulize the sample solution into a fine mist as the sample solution exits the ESI or APCI nozzle.
sheath gas flow rate The rate of flow of sheath gas into the API source. A measurement of the relative flow rate (in arbitrary units) that needs to be provided at the sheath gas inlet to provide the required flow of
to the ESI or APCI nozzle.
sheath gas inlet An inlet in the API probe where
sheath gas is introduced into the probe.
sheath gas plumbing The gas plumbing that delivers
to the ESI or APCI nozzle.
sheath gas pressure The rate of flow of sheath gas
(nitrogen) into the API source. A measurement of the relative flow rate (in arbitrary units) that needs to be provided at the sheath gas inlet to provide the required flow of inner coaxial nitrogen gas to the ESI or APCI nozzle. A software-controlled proportional valve regulates the flow rate.
.
sheath gas valve A valve that controls the flow of
sheath gas into the API source. The sheath gas valve is
controlled by the data system.
signal-to-noise ratio (S/N) The ratio of the signal height (S) to the noise height (N). The signal height is the baseline corrected peak height. The noise height is the peak-to-peak height of the baseline noise.
SIM See selected ion monitoring (SIM) scan type .
G-10
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
skimmer A vacuum baffle between the higher pressure capillary-skimmer region and the lower pressure region. The aperture of the skimmer is offset with respect to the bore of the ion transfer capillary.
source CID A technique for fragmenting ions in an
atmospheric pressure ionization (API)
source.
Collisions occur between the ion and the background gas, which increase the internal energy of the ion and stimulate its dissociation.
SPI See
Serial Peripheral Interface (SPI) .
SRM See selected reaction monitoring (SRM) scan type .
sweep gas Nitrogen gas that flows out from behind the sweep cone in the API source. Sweep gas aids in solvent declustering and adduct reduction.
See also sweep gas flow rate .
sweep gas flow rate The rate of flow of sweep gas into the API source. A measurement of the relative flow rate (in arbitrary units) to provide the required flow of nitrogen gas to the sweep cone of the API source.
.
syringe pump A device that delivers a solution from a syringe at a specified rate.
T
T Tesla
target compound A compound that you want to identify or quantitate or that a specific protocol (for example, an EPA method) requires that you look for.
Target compounds are also called analytes, or target analytes.
TIC See total ion current (TIC)
.
TMP See
.
Torr A unit of pressure, equal to 1 mm of mercury and
133.32 Pa.
total ion current (TIC) The sum of the ion current intensities across the scan range in a mass spectrum.
tube lens offset The voltage offset from ground that is applied to the tube lens to focus ions toward the opening of the skimmer.
Thermo Fisher Scientific
Glossary: skimmer–vacuum manifold
.
Tune Method A defined set of mass spectrometer tune parameters for the ion source and mass analyzer. Tune methods are defined by using the instrument software’s tune window and saved as tune file.
A tune method stores tune parameters only.
(Calibration parameters are stored separately, not with the tune method.)
tune parameters Instrument parameters whose values vary with the type of experiment.
turbomolecular pump A vacuum pump that provides a high vacuum for the mass spectrometer and detector system.
TWA time weighted average
U
u atomic mass unit
UHV ultra high vacuum
ultra-high performance liquid chromatography
(U-HPLC) See
high performance liquid chromatography (HPLC) .
Ultramark 1621 A mixture of perfluoroalkoxycyclotriphosphazenes used for ion trap calibration and tuning. It provides ESI singly charged peaks at m/z 1022.0, 1122.0, 1222.0, 1322.0, 1422.0,
1522.0, 1622.0, 1722.0, 1822.0, and 1921.9.
UMR Universal Mass Range
V
V volt
V AC volts alternating current
V DC volts direct current
vacuum manifold A thick-walled, aluminum chamber with machined flanges on the front and sides and various electrical feedthroughs and gas inlets that encloses the API stack, ion optics, mass analyzer, and ion detection system.
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
G-11
Glossary: vacuum system–XML (Extensible Markup Language)
vacuum system Components associated with lowering the pressure within the mass spectrometer. A vacuum system includes the vacuum manifold, pumps, pressure gauges, and associated electronics.
vent valve A valve that allows the vacuum manifold to be vented to air or other gases. A solenoid-operated valve.
vol volume
W
w width
W watt
WEEE European Union Waste Electrical and
Electronic Equipment Directive. Provides guidelines for disposal of electronic waste.
X
XML (Extensible Markup Language) A general-purpose markup language that is used to facilitate the sharing of data across different information systems, particularly via the Internet.
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Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Index
Numerics
00101-08-00006
00101-08500
00101-15500
00101-2500
00109-02-00020
00301-01-0013
00301-15517
0690280
119265-0003
119283-0001
119683-0100
12 pin feedthrough harness
location
120320-0030
1275730
1275740
1284050
23827-0008
23827-0009
32000-60340
3814-6530
98000-20060
98000-62006
–
98000-62008
A
A0301-15101
Active Pirani gauge (APG)
active temperature control
actual vial temperature
aluminum oxide abrasive, number 600
analog signals
analyzer chamber temperature
system
temperature
angiotensin I
anti-aliasing filters
API source safety interlock switch
settings
applicators, cotton-tipped
Automatic Gain Control (AGC)
autosampler
–
auxiliary gas
Thermo Fisher Scientific axial ion ejection
axial oscillations
B
bakeout controls
timer
,
bakeout procedure indication
starting
stopping
,
base studs
bath gas
bayonet guide
bayonet lock
bayonet pin
buck/boost transformer
burnout, of filament
buttons
Display Status View
On/Standby
system bakeout
C
cable tie
calibration parameters
CAN bus
cartridge heaters
central electrode location
power supply box
voltages
,
central electrode power supply board cooling
diagnostic LEDs
location
central electrode pulser board diagnostic LEDs
location
ceramic lens holder
ceramic spacer
charge-sign independent trapping (CSIT)
,
circuit breakers
cleaning instrument surface
ion source block
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) I-1
Index: D–E ion source lens assembly
ion volume
CLT compartment
CLT Offset connector
CLT RF main board diagnostic LEDs
function
location
,
CLT RF unit
CLT RF voltage
Cold Ion Gauge
,
collision gas
C-Trap
HCD
inlet
linear trap
Communication LED
communication link
condenser filter, of recirculating chiller
connector, to ETD Interface board
control elements inlet valve
instrument
control panel
control unit, for turbopumps
Convectron gauge
ETD Module
linear trap
cooling water circuit
flow control sensor
properties
temperature
cover lid, for bakeout controls
C-Trap description
gas supply
cycle time
D
data acquisition analog board diagnostic LEDs
layout
location
,
data acquisition digital PCI board diagnostic LEDs
layout
location
–
data acquisition unit
data communication
data system communication
connection
log file
I-2
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
DC HV Supply PCB
default values, of tune and calibration parameters
deflector electrode (DE) voltages
Display Status View button
distilled water
drip pan, of ETD forepump
dynamic range, of instrument
E
E-clips
electric power, for recirculating chiller
electrodynamic squeezing
electronic boards data acquisition analog
data acquisition digital PCI
instrument control
ion optic supply
left side
power distribution
right side
electronic connections, to linear trap
emergency shutdown
entry housing
error messages
ETD main access panel
maintenance
reagent carrier gas supply
ETD Control PCB
ETD forepump cabinet
connections
electrical cord
power supply
weight
ETD Ion Optic Supply board
–
ETD Module forepump receptacle
Heater Control PCB
Interface board
,
ion gauge
location
maintenance
power panel
power receptacle
power supply
power switch
service switch
Standby condition
ETD power module function
panel
ETD Reagent Kit
ETD side access panel
Thermo Fisher Scientific
interlocks
removing
ETD turbopump connections
controller
function
location
maintenance
Ethernet
evacuating, after a complete shutdown
Exactive Installation Kit
exhaust hose
system
external calibration
external connections
extracting voltage
extraction, of ion packets
F
fan filters
ferrules, tightening
filament assembly
,
burnout
emission current
function
wire
filter, for cooling water
fingerprints, removing
flatapole
flow control sensor
UHP nitrogen
,
flow rates cooling water
HCD collision gas
flow restrictors
fluid bag filter, of chiller
fluoranthene
focusing potentials
foreline hose connection
forepump electrical cord
forepumps cabinet
effluent
linear trap
–
location
,
oil level
oil mist filters
power supply
switches
forevacuum tube
front panel
Thermo Fisher Scientific
Index: F–H front side, LEDs
FT Electronics switch functions
location
fused silica tubing from lower oven
from upper oven
in ETD Module
G
gas ballast
gas pressures
gas supply
C-Trap
helium
nitrogen
schematics
gas valves
gate voltage
gloves
ground wires
guide bar function
,
handle
opening
H
handling, hot pump oil
harmonic oscillations, in Orbitrap
hazardous materials
HCD collision gas port
flow rate, of collision gas
fragment spectra
housing
HCD cell description
supply voltages
heated dual restrictor enclosure
heated transfer line
heater control
Heater Control PCB
–
heater ring
heating element
helium gas capillary
gas pressure
inlet
purging the line
supply
high voltage power supply board diagnostic LEDs
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
I-3
Index: I–L function
layout
location
,
voltages
high voltage, at PCBs
I
image current
,
inlet port, for UHP nitrogen
inlet valve block
knob
,
lever
,
plug
,
seal kit
seal tool
solenoid
installation kit, for the reagent inlet module
instrument controls
diagnostics
forevacuum measurement
high vacuum measurement
operating voltage
parts
rear side
right side
shutdown
switching off
instrument control board diagnostic LEDs
location
,
,
software status LEDs
internal calibration
internal computer add-ons
data communication
location
,
rebooting
timer
ion dephasing
ion gauge, for ETD Module
ion optic supply board diagnostic LEDs
location
ion optics
ion oscillation
ion packets
ion source assembly
block
,
filament
filament assembly
lens assembly
lenses
I-4
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
PCB
,
schematics
springs
ion trajectory
ion volume alignment arrow
cleaning
key thumbscrew
location
temperature
ion volume assembly, cleaning frequency
ion volume tool alignment arrow
components
handle
,
usage
,
ionization techniques
ions detection
electrodynamic squeezing
image current
packet shape
L
laboratory exhaust system
gas supplies
ventilation
LC
leak checking
LEDs power control
system status
vacuum
left side panel, of instrument
lens voltages
line power
linear trap collision gas
Communication LED
connections
description
electronics
forepumps
,
LEDs
location
,
maintenance
Power LED
,
power panel
Reset button
System LED
turbopump
Vacuum LED
vacuum measurement
vent valve
–
Thermo Fisher Scientific
Link Port Signal line
log file
lower oven
M
magnet support
magnet yoke
magnets, in ion source
main circuit breaker
main power switch function
location
Off position
securing
status
main RF supply
mains failure
,
mains supply for ETD Module
for instrument
maintenance
API source
cooling circuit
linear trap
procedures
reagent ion source
recirculating chiller
vacuum system
mass accuracy
mass range
Material Safety Data Sheet (MSDS)
micro controller
MS/MS
multiple socket outlets
N
nitrogen
CLT
connection to source
cooling gas
gas flow
inlet
pressure
pressure regulator
supply
tubing
venting
O
octapole RF voltages
oil
Thermo Fisher Scientific
Index: M–P level
mist filters
On/Standby button
operating vacuum
Orbitrap applied voltages
central electrode
chamber
control LEDs
detection electrodes
differential pumping
electrodes
Instrument Control board
ion extraction
ion trajectories
layout
lenses
measuring principle
voltages
,
output current, of preamplifier
P
padlock, for main power switch
parameters calibration
default values
tune
part numbers
Peltier elements function
,
location
temperature
water supply
Penning gauge
peripherals power outlet
Pirani gauge
,
,
position, of inlet valve lever
power connector
connector for linear trap
control LEDs
control panel
panel
supply for linear trap
power distribution operating states
resetting
working modes
power distribution board control
function
layout
location
power supply
status LEDs
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
I-5
Index: Q–S power fail detector
Power LED
power module
power outlet, for peripheral devices
–
power supply data system
ETD Module
power supply 1 board diagnostic LEDs
layout
location
,
power supply 2 board diagnostic LEDs
location
preamplifier cooling
diagnostic LEDs
location
,
,
pressure readings, in ETD Module
pressure regulator location
nitrogen
preventive maintenance
printed circuit boards
proton transfer reactions
pumping, the system
pumps exhaust
forevacuum
manuals
oil mist filters
purging, helium line
Q
quadrupole mass filter
quality, of vacuum
R
reagent heaters
reagent inlet cover
reagent inlet source heating unit
location
reagent ion source cleaning frequency
description
flow restrictors
maintenance
maintenance tools
Reagent Ion Source dialog box
Reagent Ion Source instrument control icon
Reagent Ion Source On check box
I-6
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) reagent vapor, in the carrier gas
reagent vials
rebooting, of instrument
recirculating chiller condenser filter
connections
description
fluid bag filter
maintenance
reflector DC voltages
removing
ETD main access panel
ETD side access panel
gases
stains
repair covering letter
replacing chiller operating fluid
filter cartridge
inlet valve components
ion source filament
operating fluid reservoir of turbopumps
reagent vials
turbopump operating fluid
reservoir, of recirculating chiller
resetting instrument
system parameters
tune and calibration parameters
resolution, of instrument
restrictor oven cover
oven heater
RF CLT main board
RF off & feedback board
RF output control
RF voltage supply
right side panel, of instrument
S
safety features
interlock switch
problem
sample inlet aperture
secondary electrons
SEM detector
serial port connector
service switch, of linear trap
setting up, conditions for operation
sheath gas
shutdown
,
signal communication
software
Thermo Fisher Scientific
debugging
function
status LEDs of the instrument control board
Tune Plus window
specifications, of instrument
spectrum
SPI bus function
termination board
,
,
spring clip thumb screw
stainless steel parts, cleaning
stains, removing
Standby condition
starting up
ETD Module
instrument
status LEDs, of power distribution board
Status View
sweep gas
switches forepumps
FT Electronics
linear trap
main power
vacuum pumps
switching on, the vacuum system
system bakeout
,
bakeout timer
buttons for bakeout
heating
pump down time
rebooting
resetting
shutdown
Standby
starting up
status LEDs
,
timing
venting
,
system status LEDs linear trap
Orbitrap Elite
T
temperature analyzer chamber
control
differential
monitoring
sensor
temperature controller board diagnostic LEDs
function
layout
Thermo Fisher Scientific
Index: T–V location
thermoelectric elements
thumbscrews
tools cleanliness
for cleaning stainless steel parts
for cleaning the ion volume
reagent ion source
top lids
ETD Module
MS
transfer chamber
transfer line bellows
heater
inlet
location
transfer multipole
trap voltage
triple gas filter
tune parameters
Tune Plus window buttons
diagnostics
turbopumps connections
controllers
error
HiPace 300
,
HiPace 80
linear trap
maintenance
TMP 1
TMP 2
TMP 3
TMP 4
vent valves
U
UHP nitrogen, inlet port
,
UHV chamber components
location
temperature control
vacuum
uninterruptible power supply (UPS)
upper control panel
user maintenance
V
vacuum compartments
deterioration
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A)
I-7
Index: W–X failure
gauges
,
quality
safety threshold
system
,
vacuum chamber
vacuum components left instrument side
right instrument side
Vacuum LED instrument
linear trap
vacuum manifold location
probe plate
Vacuum Pumps switch
vacuum system controls
heating
moisture
vent valve control
function
,
linear trap
nitrogen supply
ventilation, in the laboratory
venting pressure
venting, the system
,
vial heaters cover
location
ribs
vial holder
vial temperature
voltage sags
W
water chiller
,
filter
hoses
ports
temperature
water cooler, for TMP
wheels, of instrument
working modes, of power distribution
working principle, of the Orbitrap
,
X
Xcalibur
I-8
Orbitrap Elite Hardware Manual (P/N 1288170, Revision A) Thermo Fisher Scientific
Thermo Fisher Scientific Inc.
81 Wyman Street
P.O. Box 9046
Waltham, Massachussetts 02454-9046
United States
www.thermoscientific.com
Part of Thermo Fisher Scientific
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