Cuvette Test LCK 381
Cuvette Test
LCK 381
LCK 381
TOC
Total organic carbon
There is no interference from:
TIC-determination:
800 mg/L: HCOO
-
500 mg/L: CH3COO
GB
Principle
Total carbon (TC) and total inorganic carbon (TIC) are converted
to carbon dioxide (CO2) by, respectively, oxidation and acidification.
The CO2 passes from the digestion cuvette through a membrane
and into the indicator cuvette. The change of colour of the indicator
is photometrically evaluated. TOC (total organic carbon) is determined
as the difference between the TC and TIC values.
-
2-
60 mg/L: SO3
Range of Application
Waste water, surface water, soils
Storage Information
The test reagents are stable at +15 to +25°C up to the expiry date
given on the package.
2-
20 mg/L: S
6 mg/L: NO2-N
Higher concentrations of these ions cause high-bias results.
TC-determination:
Special note
1. Contamination by ambient air
Never leave cuvette open, because carbon dioxide in the
ambient air can cause high bias. Cuvettes must only be
opened when necessary (e.g. to add sample) and must be
closed or further processed immediately afterwards.
2. How to use the powder dispenser
Screw powder dispenser on to digestion reagent A (LCK 381 A).
Invert so that powder dispenser is under the reagent and
shake. This causes the dispensing chamber to be filled.
Position the centring recess of the powder dispenser above
the TC cuvette and add one dose. Close digestion reagent A
(LCK 381 A) immediately with original cap.
3. Labelling the digestion cuvettes
If several samples are analysed simultaneously, label them so
that the TC and TIC cuvette combinations of the same sample
can be recognised.
4. Thermostat
Heat the thermostat to 100°C (check the temperature – higher
temperatures lead to dangerous excess pressure). When this
temperature has been reached, insert the cuvette combinations
and start the reaction time (2h) again. Insert cuvette combinations only in the small shafts in the thermostat. Do not insert
them in the large shaft with reducing sleeves.
5. Disposal
Do not screw the cuvette combinations apart when the
analysis has been completed, but press them back into the
blister pack in combination with the indicator cuvette upwards.
Interferences
If the TC and/or the TIC results are higher than the upper limit of the
measuring range, the calculated TOC result which is displayed may
be within the measuring range.
In this case and generally the measurement results must be subjected
to plausibility checks (dilute and/or spike the sample).
Use only carbon-free double-distilled water to dilute the sample.
The ions listed in the table have been individually checked up to the
given concentrations. Cumulative effects and the influence of other
ions have not been determined by us.
5000 mg/L: Cl
2+
2000 mg/L: Ca , Mg
2+
1000 mg/L: NH4-N
Higher concentrations of these ions cause low-bias results.
pH/Temperature
The pH of the sample must be between pH 4 and pH 10. The
temperature of the sample and reagents must be between 15 and
25°C.
Safety Advice
On grounds of quality and reliability, the analysis should be carried
out only with original HACH LANGE accessories.
NB:
Be sure to set the required temperature to 100ºC (at 148ºC the
cuvette combinations may break apart).
The digestion conditions are such that oxygen is formed, and
this causes a rise in pressure in the cuvette combinations. If the
cuvette combinations are subjected to strong mechanical stress
after the digestion reaction, e.g. if they suffer a blow or a fall,
they may shatter. In this case glass splinters may cause injury.
CADAS 100 (LPG 185 / ≥ LPG 210)
If this test is not already stored in your instrument please ask your
HACH LANGE Agency for programming instructions.
AE 381 K / Druckfarbe schwarz / 1
Procedure
LCK 381
Applies to all types of photometer
TOC Total organic carbon
LCK 381
Procedure
Applies to all types of photometer
Edition 96/03
Edition 96/03
TOC Total organic carbon
Please read carefully the information under the heading ”Special note“.
Please read carefully the information under the heading ”Special note“.
Carry out each TC determination in sequence in order to avoid
contamination by ambient air (diagram A – E).
Carry out each TIC determination in sequence in order to avoid
contamination by ambient air (diagram F – I).
1. TC determination (see diagram A - E)
2. TIC determination (see diagram F - I)
Into the TC cuvette dispense and pipette:
Digestion reagent A (LCK 381 A)
Sample
Into the TIC cuvette pipette:
1 dose of powder
0.2 mL
Close TC cuvette with original cap and invert a few times. Close the
indicator cuvette very tightly with the membrane double-cap (screw on
the membrane double-cap in such a way that the barcode label is on the
lower half of the cap). Immediately screw the prepared indicator cuvette
tightly on to the TC cuvette.
NB: Cuvettes must be held vertically and must not be inverted! The
sample must not come into contact with the membrane of the double-cap!
Sample
1 mL
Close TIC cuvette with original cap and invert a few times. Close the
indicator cuvette very tightly with the membrane double-cap (screw on
the membrane double-cap in such a way that the barcode label is on the
lower half of the cap). Immediately screw the prepared indicator cuvette
tightly on to the TIC cuvette.
NB: Cuvettes must be held vertically and must not be inverted! The
sample must not come into contact with the membrane of the double-cap!
3. Heat (see diagram J + K)
Place the cuvette combinations in the preheated thermostat with the
coloured indicator cuvette upwards, and heat for 2 h at 100°C. For TOC
determination the TC and TIC cuvette combinations must be heated
simultaneously in the thermostat. Allow to cool to room temperature
(indicator cuvette upwards).
4. Evaluation (see diagram L + M)
First clean the outside of the indicator cuvette of TC cuvette combination
thoroughly (NB: when you clean the cuvette, be careful not to turn it
in such a way that the cuvette combination could accidentally be
screwed apart). Place in photometer and measure – 1. Measurement.
Then clean the outside of the indicator cuvette of TIC cuvette combination
thoroughly (NB: when you clean the cuvette, be careful not to turn it
in such a way that the cuvette combination could accidentally be
screwed apart). Place in photometer and measure – 2. Measurement.
Press the used cuvette combinations back into the blister pack (do not
unscrew cuvette combinations!).
Data table
LCK 381
LP2W
96/03
TC • F1 = 0 • F2 = 1197 • K = -245.6
TIC • F1 = 0 • F2 = 262.8 • K = -45.94
CADAS 30/50
96/03
TC/TIC • λ: 435 nm • Pro.: 11 • F1 = 1176 • F2 = 0 • F3 = 256.9 • F4 = 0 •
K1 = -283.1 • K2 = -53.05
CADAS 30S/50S
96/03
TC/TIC • λ: 435 nm • Pro.: 11 • F1 = 1178 • F2 = 0 • F3 = 256.9 • F4 = 0 •
K1 = -283.2 • K2 = -53.05
ISIS 6000/9000
96/03
TC/TIC • λ: 430 nm • Pro.: 11 • F1 = 1193 • F2 = 0 • F3 = 259.4 • F4 = 0 •
K1 = -298.1 • K2 = -56.43
CADAS 100 / LPG 185
96/03
TOC/TC/TIC • λ: 435 nm • F1 = 1165 • F2 = -238.9 • F3 = 253.8 •
F4 = -43.16 • F5 = 1 • F6 = -1
CADAS 100 / ≥ LPG 210
TOC/TC/TIC • λ: 435 nm • F1 = 1165 • F2 = -238.9 • F3 = 253.8 •
F4 = -43.16
LCK 381
LASA 1 / plus
Edition 96/03
TOC Total organic carbon
Evaluation
1. Press ”Mode“ key and check program control number:
–– : 34
2. Insert program filter 440 nm.
3. Select test with ”Mode“ key.
4. Insert indicator cuvette of the TC cuvette combination.
5. Insert indicator cuvette of the TIC cuvette combination.
20 sec display = TC
20 sec display = TIC
20 sec display = TOC
or by pressing the key the results for TC, TIC and TOC
can be called up immediately one after another.
∗
96/03
Parameter
TOC
TC
TIC
Display
TOC LCK 381
Meas. range
60 – 735 mg/L
75 – 750 mg/L
15 – 150 mg/L
LCK 381
LASA 10 / 20
Edition 96/03
TOC Total organic carbon
LCK 381
LASA 30
Edition 96/03
TOC Total organic carbon
Evaluation
Evaluation
1. Press any key.
2. Check program control number: –– : 34
3. Select test with ↑ or ↓ key.
4. Insert indicator cuvette of the TC cuvette combination.
5. Insert indicator cuvette of the TIC cuvette combination.
20 sec display = TC
20 sec display = TIC
20 sec display = TOC
or by pressing the key ↓ the results for TC, TIC and TOC
can be called up immediately one after another.
1. Insert filter 440 nm.
2. Select »Dr. Lange« mode.
3. Select test number (see below).
4. Control number must be 2.
5. Insert indicator cuvette of the TC cuvette combination
and press green key.
6. Insert indicator cuvette of the TIC cuvette combination
and press green key.
Parameter
TOC
TC
TIC
Parameter
TOC
TC
TIC
Display
TOC LCK 381
Meas. range
60 – 735 mg/L
75 – 750 mg/L
15 – 150 mg/L
The result is displayed in TC, TIC and TOC.
LCK 381
LP2W
Edition 96/03
TOC Total organic carbon
Test-No.
381
Photometer with
TOC Total organic carbon
Meas. range
60 – 735 mg/L
75 – 750 mg/L
15 – 150 mg/L
LCK 381
Barcode-System
Edition 96/03
Evaluation
Evaluation
TC determination
1. Check program control number:
–– : 34 (CADAS 30S/50S, ISIS 9000)
–– : 38 (CADAS 200)
2. Insert indicator cuvette of the TC cuvette combination.
3. Insert indicator cuvette of the TIC cuvette combination.
1. Insert program filter 435 nm.
2. Press ”Tests“ key until display (see below) appears.
3. Control number must be 8.
4. Insert blank-value cuvette (distilled water)
and press ”Null“ (zero) key.
5. Insert indicator cuvette of the TC cuvette combination
and press ”Ergebnis“ (result) key.
Make a note of the display – TC
TIC determination
1. Insert program filter 435 nm.
2. Press ”Tests“ key until display (see below) appears.
3. Control number must be 4.
4. Insert blank-value cuvette (distilled water) and
press ”Null“ (zero) key.
5. Insert indicator cuvette of the TIC cuvette combination and
press ”Ergebnis“ (result) key.
Make a note of the display – TIC
CADAS 30S/50S, ISIS 9000:
The results for TC, TIC and TOC can be called up by
pressing the key under the symbol → .
CADAS 30S/50S/200, LASA 50/100, XION 500:
The result is displayed in TC, TIC and TOC.
Parameter
TOC
TC
TIC
Meas. range
60 – 735 mg/L
75 – 750 mg/L
15 – 150 mg/L
Calculation of the TOC concentration:
TC - TIC = mg/L TOC
Parameter
TOC
TC
TIC
Display
TC LCK 381
TIC LCK 381
Meas. range
60 – 735 mg/L
75 – 750 mg/L
15 – 150 mg/L
AE 381 K / Druckfarbe schwarz / 2
CADAS 200 Basis , ISIS 6000
LCK 381
Edition 96/03
TOC Total organic carbon
LCK 381
CADAS 100 (LPG 185)
Edition 96/03
TOC Total organic carbon
Evaluation
Evaluation
1. Check program control number:
–– : 38 (CADAS 200)
–– : 34 (ISIS 6000) ⇒ Select »CUVETTE TEST« mode.
2. Select test number (see below).
3. Control number must be 2.
4. Insert indicator cuvette of the TC cuvette combination
and press green key.
5. Insert indicator cuvette of the TIC cuvette combination
and press green key.
1. Select »TEST« mode.
2. Select symbol (see below).
3. Select symbol » > «.
4. Check factors and measuring wavelength in
memory »Mem«.
5. Insert blank-value cuvette (distilled water)
and press ”NULL“ (zero) key.
6. Insert indicator cuvette of the TC cuvette combination
and press ”MESS“ (measure) key.
7. Insert blank-value cuvette (distilled water)
and press ”NULL“ (zero) key.
8. Insert indicator cuvette of the TIC cuvette combination
and press ”MESS“ (measure) key.
ISIS 6000:
The results for TC, TIC and TOC can be called up by
pressing the key under the symbol → .
The result is printed out in TC, TIC and TOC.
CADAS 200:
The result is displayed in TC, TIC and TOC.
Parameter
TOC
TC
TIC
Test-No.
381
CADAS 100 ( ≥ LPG 210)
Meas. range
60 – 735 mg/L
75 – 750 mg/L
15 – 150 mg/L
LCK 381
Edition 96/03
TOC Total organic carbon
If more than one sample is to be measured start the next
evaluation at point 5.
Parameter
TOC
TC
TIC
Symbol
$ 381
Meas. range
60 – 735 mg/L
75 – 750 mg/L
15 – 150 mg/L
LCK 381
DR 1900
Edition 96/03
TOC Total organic carbon
Evaluation
Evaluation
1. Select »TEST« mode.
2. Select symbol (see below).
3. Control number must be 8.
4. Insert blank-value cuvette (distilled water)
and press ”NULL“ (zero) key.
5. Insert indicator cuvette of the TC cuvette combination
and press ”MESS“ (measure) key.
6. Insert indicator cuvette of the TIC cuvette combination
and press ”MESS“ (measure) key.
1. Select »Barcode Programs«.
2. Select test number (see below).
3. Control number must be 2.
4. Insert indicator cuvette of the TC cuvette combination
and press »Read 1«.
5. Insert indicator cuvette of the TIC cuvette combination
and press »Read 2«.
The result is printed out in TC, TIC and TOC.
Parameter
TOC
TC
TIC
If more than one sample is to be measured start the next
evaluation at point 5.
Parameter
TOC
TC
TIC
Symbol
381
Meas. range
60 – 735 mg/L
75 – 750 mg/L
15 – 150 mg/L
The result is displayed in TC, TIC and TOC.
Test-No.
381
Meas. range
60 – 735 mg/L
75 – 750 mg/L
15 – 150 mg/L
Cuvette Test
LCK 381
Procedure
Applies to all types
of photometer
TCDetermination
TICDetermination
1x
TC
Transfer 1x
digestion
reagent A into
the TC cuvette.
A
Pipette 0.2 mL
sample
into the TC
cuvette.
Pipette 1 mL
sample
into the TIC
cuvette.
TIC
TC
F
D
H
Indicator
E
Indicator
Screw the
prepared indicator
cuvette tightly
on to the
TC cuvette.
I$E"
Hold
cuvette combination
vertically.
TC
Do not invert.
Indicator
G
Indicator
C
Close
indicator
cuvette very
tightly with
membrane
double-cap.
(barcode
label must
be on
lower half).
I$E"
I$E"
TC
TIC
Heat both cuvette
combinations
simultaneously in
the preheated
thermostat
for 2 h at 100°C.
I$E"
TC
TC
1. Measurement
Screw the
prepared indicator
cuvette tightly
on to the
TIC cuvette.
I$E"
Hold
cuvette combination
vertically.
TIC
Do not invert.
100 C
120 MIN
THERMOSTAT
I
J
Allow to cool to
room temperature.
Indicator
Invert TC cuvette combination, clean
the outside of indicator cuvette
thoroughly, insert in photometer
and measure.
Indicator
Indicator
Close
TIC cuvette
with original
cap and
invert a
few times.
Invert TIC cuvette combination, clean
the outside of indicator cuvette
thoroughly, insert in photometer
and measure.
I$E"
TIC
K
Indicator
Indicator
I$E"
3 81
TIC
2. Measurement
I$E"
Close
indicator
cuvette very
tightly with
membrane
double-cap
(barcode
label must
be on
lower half).
I$E"
Close
TC cuvette
with original
cap and
invert a
few times.
I$E"
B
L
3 81
M
AE 381 K / Druckfarbe schwarz / 2a
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