ABBOTT CELL-DYN 1800 Hematology Analyzer Service & Support Manual

CELL-DYN 1800 Automated Hematology Analyzer is a complex system. Analyzer performance depends on several components that together make up the complete hematology system. The system comprises of the following components and subsystems: Flow Panel System, Fluid Power Supply, Reagent Inlet Panel, Syringe Drive Assemblies, Electronics Card Cage, LCD Display System, Touchpad (Membrane) Keyboard and Power Supply Assembly.

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ABBOTT

CELL-DYN 1800

Automated Hematology Analyzer

Service & Support Manual

ABBOTT CD1800 SM

Theory of Operation ............................................................................................................................................................................ 4

System Overview.................................................................................................................................................................... 4

Major Subsystem Descriptions........................................................................................................................................... 6

Circuit Descriptions ............................................................................................................................................................. 15

Signal Processor Module (SPM)....................................................................................................................................... 18

Cell Count Module (CCM) ................................................................................................................................................... 21

Troubleshooting................................................................................................................................................................................. 31

Troubleshooting Charts...................................................................................................................................................... 33

Raw Data Description.......................................................................................................................................................... 40

CCM On-Board Diagnostic LEDs...................................................................................................................................... 41

CPU Hardware/Software Configuration .......................................................................................................................... 44

Service Special Commands............................................................................................................................................... 45

Sample Probe Description ................................................................................................................................................. 49

CELL-DYN 1800 Error Messages...................................................................................................................................... 60

Software Commands and Sequence ............................................................................................................................... 72

Engineering Drawings and Schematics ............................................................................................................................................. 76

CELL-DYN 1800 PCB Reference .................................................................................................................................................... 77

Removal & Replacement .................................................................................................................................................................. 96

Service Equipment Required............................................................................................................................................. 96

Covers (CD18-A1) .................................................................................................................................................................... 97

Flow Panel (CD18-B1)............................................................................................................................................................ 105

Fluid Power Supply (CD18-C1).............................................................................................................................................. 115

Syringe Assembly (CD18-E1)................................................................................................................................................. 119

RR-E1.04 Sample Syringe Driver Assembly .................................................................................................................... 125

RR-E1.06 Lyse Syringe Driver Assembly..................................................................................................................... 127

Electronics / Card Cage (CD18-F1) ........................................................................................................................................ 129

RR-F1.01 PAM (Pre-Amplifier Module) ............................................................................................................................. 129

RR-F1.02 MPM (Motor Processor Module) Board .......................................................................................................... 131

RR-F1.03 CDM (Cable Distribution Module) Board ........................................................................................................ 133

RR-F1.04 Hard Disk Drive................................................................................................................................................... 135

RR-F1.05 Floppy Disk Drive ............................................................................................................................................... 138

RR-F1.06 Signal Processor Module (SPM)...................................................................................................................... 140

RR-F1.07 Cell Count Module (CCM) ................................................................................................................................. 142

RR-F1.08 Data Link Adapter (DLA) ................................................................................................................................... 143

RR-F1.09 Single Board Computer (SBC) ......................................................................................................................... 145

RR-F1.10 Card Cage Backplane PCB .............................................................................................................................. 147

LCD/Keyboard (CD18-G1)..................................................................................................................................................... 149

Power Supply (CD18-H1) .................................................................................................................................................... 151

Verification Procedures ................................................................................................................................................................... 154

VP-01 Preparation for Alignment and Verification ................................................................................................................. 154

VP-01 Preparation for Alignment and Verification..................................................................................................... 154

Test Equipment and Supplies Required....................................................................................................................... 154

Preparation for Alignment/Verification ......................................................................................................................... 155

Order of Alignment/Verification...................................................................................................................................... 156

VP-02 Decontamination .................................................................................................................................................... 157

VP-03 Vacuum and Pressure Adjustments.................................................................................................................. 158

Regulator Alignment.......................................................................................................................................................... 159

Pressure Adjustment (0.5 psi)......................................................................................................................................... 160

Pressure Verification (High) ............................................................................................................................................ 161

Vacuum Adjustment (8 inch) ........................................................................................................................................... 161

VP-04 Metering System Timing Adjustments - RBC and WBC .............................................................................. 162

Metering Timing Fault Report.......................................................................................................................................... 163

RBC Metering System Timing Adjustment .................................................................................................................. 165

WBC Metering System Timing Adjustment ................................................................................................................. 166

VP-05 CMOS Setup Verification/Adjustment............................................................................................................... 167

VP-06 Card Cage Backplane Test Points ..................................................................................................................... 171

VP-07 Cable Distribution Module Test Points............................................................................................................. 172

VP-08 Pre-Amplifier Module (PAM) Adjustment ......................................................................................................... 173

VP-09 Signal Processor Module (SPM) Verification/Adjustment ........................................................................... 176

RBC Gain .............................................................................................................................................................................. 178

RER Adjustment ................................................................................................................................................................. 179

WBC Gain ............................................................................................................................................................................. 182

PLT Gain ............................................................................................................................................................................... 184

VP-10 Diluent and Sample Verification/Adjustment ........................................................................................................ 187

Diluent Volume Verification ............................................................................................................................................. 188

Sample Volume Verification ............................................................................................................................................ 188

VP-11 Stepper Motor Power Test and Verification .................................................................................................... 189

VP-12 Sample Probe Alignment Check ........................................................................................................................ 190

VP-13 Serial Transmit to LIS Verification ..................................................................................................................... 191

VP-14 Hard Disk Drive Setup and Verification............................................................................................................ 194

VP-15 Software Installation/Upgrades .......................................................................................................................... 197

Theory of Operation

System Overview

The CELL-DYN 1800 Automated Hematology Analyzer is a complex system. Analyzer performance depends on several components that together make up the complete hematology system. The system is comprised of the following components and subsystems:

• Flow Panel System [3]

• Fluid Power Supply [2]

• Reagent Inlet Panel [6]

• Syringe Drive Assemblies [7]

• Electronics Card Cage [8]

• LCD Display System [4]

• Touchpad (Membrane) Keyboard [5]

• Power Supply Assembly [1]

Flow Panel

The Flow Panel consists of tubing, solenoid valves, and other hardware components used for sample aspiration, dilution, measurement and waste removal.

Fluid Power Supply

The Fluid Power Supply contains the vacuum and pressure pumps, accumulators, waste bottles, and associated solenoids and hardware.

Reagent Inlet Panel

The Reagent Inlet Panel provides connections for incoming reagents and outgoing waste. The Lyse solenoid is also mounted on this panel.

Syringe Drive Assembies

The Syringe Drive Assemblies include the Sample Syringe for aspirating samples, the Diluent Syringe for supplying Diluent throughout the Flow Panel, and the Lyse Syringe for dispensing Lyse to the WBC transducer for the HGB measurement process.

Electronics Card Cage

The electronics card cage, with associated PCBs, provides command and control signals for the various electronic components of the instrument. This assembly contains the Backplane PCB, Cell Count Module, Signal Processor Module,

Data Link Adaptor and Single Board Computer.

LCD Display Screen and Keyboard

The LCD Display Screen provides a visual data display and the keyboard provides data input by the operator.

Power Supply Assembly

The Power Supply Assembly provides an AC and DC voltage source to various components on the CELL-DYN 1800 System.

The DC Regulator PCB provides power to the Backplane PCB for use with the digital circuitry on various PCBs. It also provides power to the Cable Distribution Module and fans.

The AC Regulator PCB provides power to the Backplane PCB (for use with the analog circuitry on various PCBs). It also provides power to the Pump Relay Module.

The +28VDC Switching Power Supply provides power for the stepper motors via the Motor Processor Module. It also provides power to initially energize solenoids.

Major Subsystem Descriptions

To aid in understanding the overall system, the electronic modules are divided into the following major functional subsystems:

• Data Interface and Control Subsystem

Subsystem

• Solenoid Motor and Pump Subsystem

• Single Board Computer Subsystem

• AC and DC Power Distribution Subsystem

Data Interface and Control Subsystem

The purpose of this subsystem is to interface the user data, control data, measurement data, and system status data in the system. This data is connected via four independent data busses:

• DLA/CCM (Data Link Adapter/Cell Count Module)

• CCM/SPM (Cell Count Module/Sample Processor Module)

• CCM/CDM (Cell Count Module/Cable Distribution Module)

• CCM/MPM (Cell Count Module/Motor Processor Module)

See the figure below for a diagram showing the data connections.

Data Interface and Control Block Diagram

When power to the instrument is turned ON, the system is operating software is loaded from the hard disk into RAM on the

SBC (Single Board Computer). The SBC then uses various handshaking signals and data bytes to communicate with the

CCM (Cell Count Module) via the DLA (Data Link Adapter).

The CCM functions as the master controller with all system functional commands residing in firmware (PROM). The CCM sends control data and receives status data from the CDM (Cable Distribution Module).

The CCM provides current control to the von Behrens RBC and WBC Transducers and the two metering PCBs and serves as the system's analog voltmeter for use in converting the HGB signal. Data is written and read via the CCM/CDM and

CCM/MPM data buses.

The CDM (Cable Distribution Module) acts as a controller for the solenoids and also interfaces data from various system sensors.

The MPM (Motor Processor Module) acts as a controller for all Stepper Motor Drive PCBs.

Measurement Subsystem

The measurement subsystem provides detection, amplification, and processing of the signals from the von Behrens

RBC/PLT Transducer, von Behrens WBC Transducer, and HGB Flow Cell. RBC/PLT and WBC metering is also included in this subsystem.

See the figure below for a diagram of the measurement process.

Measurement Block Diagram

The PAM (Pre-Amplifier Module) supplies constant current for the von Behrens RBC/PLT and WBC Transducers and HGB

LED voltage.

The RBC/PLT and WBC cell pulses are input to the PAM where they are amplified and routed to the SPM (Signal Processor

Module).

When the SPM receives signals from the RBC/PLT and WBC, the following occurs:

• The RBC/PLT signal is amplified (gain) and split into independent RBC and PLT signals.

• The WBC signal is amplified (gain).

• The RBC signal is routed to the cell editing circuitry.

• Cell editing is performed on the RBC signal to eliminate invalid RBC pulses.

The SPM discriminates cell size by converting pulse height to a proportional digital value. The amplitude of each valid pulse is measured by a fast A/D converter then sent across the data bus to the CCM.

The A/D data for RBC, PLT, and WBC are individually divided by the CCM into 256 discrete size channels. The cell count in each channel is accumulated in discrete memory locations and is used to generate count data, percentage data, and histogram data for RBC, PLT, WBC, and other derived parameters.

Signals from the upper and lower detectors on the RBC/PLT and WBC metering PCBs are converted to TTL levels by comparators on the CDM. The signals are then routed to the CCM where they are used to control RBC/PLT and WBC sample timing.

The HGB analog signal from the HGB Flow Cell is captured by the PAM where it is amplified and routed to the CCM. The

HGB signal is then measured and converted to a digital format by a voltmeter-A/D converter.

Solenoid, Motor Drive, and Pump Subsystem

Solenoid control commands reside in firmware on the CCM. These commands are sent to the CDM where they are multiplexed to the appropriate SDM (Solenoid Drive Module). The SDM then provides the current to open and close individual drive solenoids.

Stepper Motor commands are handled in much the same manner as described above. However, the final multiplexing of the

Stepper Drive PCBs is controlled by the MPM.

There are two pressure pumps and one vacuum pump in the CELL-DYN 1800 System. These pumps are described as follows:

• A pressure pump provides air to bubble-mix samples in the Pre-Mixing Cup and the mixing chambers of the von

Behrens RBC/PLT and WBC Transducers. A pressure regulator regulates the 0.5 psi in the pressure accumulator for this process.

• An unregulated pressure pump provides air to push waste from the waste bottles inside the instrument to the waste container attached to the instrument and to apply back pressure to clear the apertures in the von Behrens RBC/PLT and WBC Transducers.

• An 8" Hg vacuum accumulator, vacuum sensor, and vacuum pump supply a constant vacuum to the entire system to transport Diluent, Detergent, and Lyse throughout the flow system and to maintain a constant vacuum to the

RBC/PLT and WBC metering tubes. A vacuum regulator maintains a constant vacuum source to both metering tubes.

See

Solenoid, Motor Drive and Pump Block Diagram

for a diagram of the solenoid and motor drive connections.

Solenoid, Motor Drive and Pump Block Diagram

Single Board Computer Subsystem

The Single Board Computer subsystem consists of the following components:

• Single Board Computer PCB

• Data Link Adapter

• Input/Output Ports (serial/parallel)

• Keyboards (PC and membrane)

Screen

• Disk Drives (hard and floppy)

The figure below illustrates the major components of the User Interface Computer.

User Interface Computer

SBC (Single Board Computer) PCB

The Single Board Computer (SBC) PCB is a complete Celeron 850 Megahertz PC computer system with 128 Megabytes of

RAM that is self-contained on one board. It utilizes a PC compatible BIOS with DOS capability that directly interfaces with the

LCD Display Screen, Hard Disk Drive, Floppy Disk Drive, PS/2 Keyboard, one (1) Parallel Port and two (2) Serial Ports. It connects directly into the Backplane PCB along with the Data Link Adapter (DLA) and utilizes the ISA bus and Backplane

PCB to communicate with one another.

DLA (Data Link Adapter)

The DLA PCB connects directly into the Backplane PCB. It provides a program-controlled data channel from the SBC to the

CCM. The DLA performs the following three functions:

• Receives measurement and analyzer status data from the CCM

• Transfers commands from the SBC to the CCM

• Sends control data from the UIC to the CCM.

Serial I/O Ports

The CELL-DYN 1800 contains two (2) Serial I/O Ports for transferring data to other computer systems:

COM1 RS-232 Data Output

COM2 Spare

Parallel I/O Port

The CELL-DYN 1800 contains one parallel I/O port for transferring data to a printer.

PC/2 Keyboard

The external 101 key PC keyboard is used to enter alphanumeric data, such as demographic information, into the instrument.

Touch Pad (Membrane) Keyboard

The touch pad (membrane) keyboard is located below the LCD Display Screen. The keyboard includes a row of eight (8) unmarked rectangular-shaped keys corresponding to labels displayed at the bottom of the screen. These keys activate the indicated function or display the indicated submenu.

LCD Display Screen (Color)

The LCD Display Screen has the following characteristics:

• Size: 8.5 x 6.4 inches (10.4 inches measured diagonally)

• Number of colors: 16

• Pixels: 640 width x 480 height (or 800 x 600)

• Backlight on/off control (software controlled screen saver)

An LCD Adapter, connected directly on the SBC PCB, drives the LCD Display Screen. The adapter supports SVGA 640 x

480 and 800 x 600 graphics modes.

Hard Disk Drive

The hard disk drive stores the User Interface Software program and the Patient Data Log.

Floppy Disk Drive

The 1.44MB 3.5" floppy disk drive is used for program installation and provides the QC (Quality Control) data upload/download capability.

Printer

The User Interface Software supports Epson ESC-P or PCL-3 languages. The printer has its own buffer and is capable of printing on 8.5" x 11" (letter size) or A4 paper size. The printer supports alphanumeric and graphics reports from stored data and screen displays.

Speaker

The PC speaker or buzzer is controlled by software and is mounted on the SBC PCB.

AC and DC Power Distribution Subsystem

The Power Supply Assembly is comprised of three components: AC Regulator PCB, DC Regulator PCB and +28VDC

Switching Power Supply. These components are mounted together as an assembly and are located at the right/rear side of the instrument.

When the system is turned on, the AC line is routed into the AC Regulator PCB and +28VDC Switching Power Supply. The

AC Regulator PCB automatically accommodates line voltages of 90 - 130VAC and 200 - 260VAC by sensing the input voltage and utilizing an internal comparator bank and power transformer to produce the 120VAC necessary for the

subsystem's function. The Power Supply Assembly then provides an AC and DC voltage source to various components on the CELL-DYN 1800 System.

+28VDC Switching Power Supply

The +28VDC Switching Power Supply provides the voltage source to the DC Regulator PCB, which the board then uses to produce other voltages. The power supply cooling fan is thermistor controlled, which means that when the internal temperature rises above 70°C, the fan operates at full speed. The fan is then turned off at 50°C and kept off until the temperature rises above 70°C.

AC Regulator PCB

The AC Regulator PCB provides the 120VAC used by the Pump Relay Module (PRM) for vacuum and pressure pump operation.

The ±12VDC (analog) is provided to the Backplane PCB, which is used by the Cell Count Module (CCM) and Signal

Processor Module (SPM). The ±12VDC is also provided to the Cable Distribution Module (CDM), Motor Processor Module

(MPM) and Pre-Amplifier Module (PAM) (for its circuitry).

The +100VDC is provided to the PAM for use in its constant current circuitry. In between the AC Regulator PCB and the PAM is the Pre Amp Filter that is used to filter out noise.

DC Regulator PCB

The DC Regulator PCB provides +5VDC, ±12VDC (digital), +14VDC and +28VDC. The +5VDC is provided to the Backplane

PCB, which is used by the SPM, CCM, Data Link Adapter (DLA) and Single Board Computer (SBC). This voltage is also used by the CDM, MPM, Hard and Floppy Disk Drives.

The ±12VDC (digital) is provided to the Backplane PCB for use on the CPU fan. The Hard and Floppy Disk Drives receive

+12VDC for their operation.

The +14VDC is provided through the CDM to the Solenoid Driver Modules (SDM) and is used to hold the solenoids closed or open for normally closed solenoids.

The +28VDC is provided to the MPM for operating stepper motors and through the CDM to the SDMs to initially close solenoids or open for normally closed solenoids. This voltage is also used for the system's internal cooling fans. The cooling fans are thermistor controlled, which means that when the ambient temperature inside the instrument drops below 25°C, the fans operate at half speed. Once the temperature rises above 25°C, the speed is increased linearly until it reaches 35°C, when the fans operate at full speed.

The DC Regulator PCB also provides +5VDC and +14VDC to the AC Regulator PCB.

Circuit Descriptions

This section contains a description of the circuitry for the following printed circuit boards:

• Pre-Amplifier Module (PAM)

• Signal Processor Module (SPM)

• Cell Count Module (CCM)

• Cable Distribution Module (CDM)

• Solenoid Driver Module (SDM)

• Motor Processor Module (MPM)

• Stepper Drive Printed Circuit Board (SDP)

• Pressure/Vacuum Regulator Module (PVRM)

• Power Supply Assembly

• Pump Relay Module (PRM)

• Single Board Computer (SBC)

• Data Link Adapter (DLA)

Pre-amplifier Module (PAM)

Note

Refer to PAM PCB Diagram .

PAM PCB Diagram

The PAM performs the following functions:

• Provides constant current control to the von Behrens RBC/PLT and WBC Transducers.

• Amplifies the initial RBC/PLT, WBC and HGB signals.

The constant current bias (+100VDC) is switched by U6, then routed to Q2 and Q3, which supplies constant current to the von Behrens RBC/PLT transducers. The RBC/PLT current is adjusted with R72. Once received, the RBC/PLT signals are initially amplified by U7, then routed to U5, where they are re-inverted and further amplified. The combined RBC/PLT signal is then routed to the SPM PCB.

Transistors Q4, Q5 and associated circuitry provide constant current for the von Behrens WBC Transducer. The WBC current is adjusted via R66. Once received, the WBC signal is initially amplified by U12, then routed to U11 where it is re-inverted and further amplified. The WBC signal is then routed to the SPM PCB.

The output of the HGB Flow Cell is amplified by U1 and U2 prior to being routed to the SPM PCB. The HGB self test and gain voltages are adjusted with R14 and R4 respectively.

Signal Processor Module (SPM)

Note

Refer to SPM Architecture .

SPM Architecture

EPLD (SPM)

The EPLD is an enhanced programmable logic device. The SPM EPLD is used to control data acquisition.

There are three state machines that run in the EPLD. One each is used for controlling data acquisition elements such as

Multiplexers and Peak/Hold Amplifiers. The inputs to the EPLD state machines are the outputs of the various Threshold and

Slope Detectors as well as Bubble and Area Comparators.

There is a data transfer state machine which controls data flow to the CCM.

Signal Processing

There are two signals from the RBC and WBC transducers that are processed through two basic data acquisition circuits.

The Platelet signal is derived from the RBC signal. There are three gain adjustments associated with each of these signals.

In addition to the three gain adjustments there is a integration adjustment for detecting pulses that are too long.

WBC Signal

• The WBC signal is received through a differential amplifier to reduce noise. There is a gain adjustment after the differential amplifier but before the Test Signal injection point.

• The WBC signal is then "Baseline Restored" to remove DC components and Baseline fluctuations due to varying duty cycle of the blood cells.

• A threshold detector signals to the EPLD the presence of valid pulses.

• Simultaneously the Bubble detector signals if the pulse is determined to be a bubble. The bubbles are much larger than pulses from blood cells. The EPLD discards the information from the pulse if it is determined to be a bubble.

• The data from the pulse is processed on the falling edge of the Threshold Detector unless the Slope Detector senses another rising edge before the falling edge of the Threshold Detector. The data is processed immediately if there is another rising edge before the falling edge of the Threshold Detector.

• The WBC Held Peak is converted and sent to the CCM on a 15µS cycle. Average pulses from the impedance transducers are 35µS.

RBC and PLT Signals

There are two modes for RBC/PLT data acquisition:

•

RBC MCV

•

RBC Count/PLT

RBC MCV

RBC MCV is for RBC MCV data only. In this mode, an integrator is enabled to determine if the cell is moving through the middle of the aperture. If the cell is not moving through the center of the aperture, the data is falsely high and the pulse is abnormally long. The integrated signal is compared to a proportion of its height. If the pulse is too long for its height, it is discarded.

RBC Count/PLT

RBC Count mode is for count information. During this run, Platelets are counted simultaneous with RBCs and no integration is used. The Platelet signal is derived from the RBC signal by an adjustable gain.

• The RBC signal is received through a differential amplifier to reduce noise. There is a gain adjustment after the differential amplifier but before the Test Signal injection point.

• The RBC signal is then "Baseline Restored" to remove DC components and Baseline fluctuations due to varying duty cycle of the blood cells. At this point an additional gain stage is added to create the Platelet signal.

• A threshold detector(s) signals to the EPLD the presence of valid pulses. The Platelet Threshold Detector is used if in

RBC Count mode.

• If in Count mode, the Platelet Lower and Upper Threshold detectors are used. If the Upper Threshold Detector is triggered, the pulse is a RBC and the RBC pulse is accumulated into the RBC Count histogram. If only the Lower

Detector is triggered, the PLT pulse is accumulated into the PLT histogram.

• The data from the pulse is processed on the falling edge of the Threshold Detector unless the Slope Detector(s) senses another rising edge before the falling edge of the Threshold Detector. If this occurs, the data is processed immediately.

• The RBC Held Peak or the PLT Held Peak is converted and sent to the CCM on a 15µs cycle.

Cell Count Module (CCM)

Note

Refer to PAM PCB Diagram .

CCM Architecture

System Clock and Microprocessor Description

The CCM uses a 16 MHz crystal as a time base. The fundamental cycle time for the MC68HC11KW1 processor is 4 MHz.

The processor has many built in functions such as:

• 16 bit address bus

• 8 bit data bus

• 7 - 8 bit multi-purpose I/O ports (CCM and SBC ports)

• Small amounts of Internal RAM and Internal EEPROM (analyzer serial number)

• Internal Timers (system timer)

• Internal UART (debug port)

EPLD (CCM)

The EPLD is an enhanced programmable logic device and is used for address decode and histogram building functions. Its program is used in coordination with the processor software.

LEDs

Note

Refer to CCM On-Board Diagnostic LEDs .

Scratch Pad RAM

This RAM is used for general purpose programming.

Histogram Memory

This RAM is used for histogram building.

Histogram Building (cell counting)

The following sequence is executed to build histograms:

1. Histogram Memory is cleared.

Counting is enabled.

This disconnects Histogram Memory from the processor buss.

3. Wait for an End of Conversion (EOC) signal from SPM.

4. At the EOC, data is read from the SPM ADC

5. The data then becomes the address for the histogram memory.

The data from the histogram memory is read.

This data is the count information for the respective pulse height.

7. The count is incremented and stored back into histogram memory.

Counting is disabled. And the histogram can be read by the processor.

HGB Measurement

A 12 bit ADC with an input multiplexer is used to measure the HGB signal from the PAM. This data is read by the processor.

Along with the HGB measurement, various DC voltages are read by the 12 bit ADC as a diagnostic.

CDM Port

The CDM port is connected directly to microprocessor ports E, G and K.

SBC Port

The SBC port is connected directly to microprocessor ports J and part of port A.

Cable Distribution Module

Note

Refer to Solenoid, Motor Drive and Pump Block Diagram .

Solenoid, Motor Drive and Pump Block Diagram

The CDM performs the following functions:

• Status Sensor Interface

• Control of Solenoid Driver Module

• Pump Relay Module interface and control

• Start Board (Touch Plate) Interface

The CDM communicates with the CCM via the CCM/CDM data bus at J2. Analog outputs of the Metering Modules are converted to TTL levels by comparators (U12) and placed directly on the CCM/CDM data bus. Signals from the Pump Relay board, Probe Position Switches, and Start Board (Touch Plate) are interfaced by Data Drivers (U5, U10).

Data is interfaced to the Solenoid Driver Modules via J32. This data is then multiplexed by One-of-Eight Decoders (U1, U2) via J3, J4, J6, J7, and J9.

Vacuum and pressure control data is latched by U14 and routed to the Pump Relay Module via J11. Pump status signals

(Vac On, Pres On) are converted to TTL levels by U3 and placed on the data bus by U5.

LED drive signals are routed to the Start Board (Touch Plate) via J17. The start signal enters at J17 and is placed on the data bus by U5.

Solenoid Driver Module (SDM)

Note

Refer to Solenoid, Motor Drive and Pump Block Diagram .

The purpose of the SDM is to provide drive current to the solenoids. Each SDM has eight Darlington drivers (Q1-Q8) which are individually controlled by data bits (D0-D7) and data latch (U3).

There are two power modes available for each solenoid - activate (+28V) and hold (+14V). This is controlled by the Hi CLK signal in conjunction with data bits (D0-D7) and the current control latch (U1).

Motor Processor Module (MPM)

Note

Refer to Solenoid, Motor Drive and Pump Block Diagram .

The MPM controls drive data to the Stepper Drive printed circuit boards and also provides self-test capability for motor winding current. The MPM is comprised of the following major circuits:

• Microprocessor

• Program Control EPROM

• I/O Peripheral Interface Adapter (PIA)

• Direct Memory Access (DMA) Control

Latches

• Motor Current Latches

• Motor Winding Self Test

Control functions of the MPM are performed by microprocessor (U5).

The operating program for the microprocessor is stored in Program Control EPROM (U3).

Data communications between the CCM and MPM are controlled by I/O PIA (U6) and serial data is interfaced via ACIA (U2) and Data Bus Connector (J1).

Phase data, motor direction, and step rate are stored in RAM (U7). This data is sent to the Motor Phase Latches under control of the DMA Control circuitry, which consists of U11, U12, U15, U16, U18, U21 and associated circuitry. The data is strobed into the appropriate Motor Phase Latch by ALG0 through ALG2.

The Motor Phase Latches U23, U26, and U29 provide phase data to the Stepper Drive printed circuit boards. Each is an 8-Bit

Addressable Latch which can control up to four Stepper Drive printed circuit boards and subsequently four Stepper Motors.

Four levels of motor current for each motor is controlled by the Motor Current Latches (U22, U25, and U28). Each latch can control up to four stepper drive printed circuit boards. Data is strobed into the appropriate latch by WR0 through WR2.

The Feedback- and Feedback+ inputs at J3 through J14 are connected, via resistors on the Stepper Drive printed circuit board, to the stepper motor windings. This allows the circuitry consisting of U30, U31, and U32 to monitor the winding current during an internal self-test. These values can be read by the CCM to isolate a defective Stepper Drive or Stepper Motor.

Stepper Drive Printed Circuit Board

Note

Refer to MPM section, blocks 20, 23, and 24 of Solenoid, Motor Drive and Pump Block Diagram .

The Stepper Drive printed circuit board consists of two PBL 3717 motor drive chips. Each chip drives a winding of the

Stepper Motor. Bits I0 and I1 are used to control four motor current levels:

• P0 - High Current

• P1 - Medium Current

• P2 - Low Current

• P3 - Current Off

Bits PH0 and PH1 control motor phase and, therefore, direction and step-rate (velocity). Feedback+ and Feedback- are used to generate a motor self-test.

Pressure/Vacuum Regulator Module

Note

Refer to Solenoid, Motor Drive and Pump Block Diagram .

Pressure (or vacuum) is sensed by a transducer that is internally configured as a Wheatstone Bridge. Transistor Q1and resistors R4 and R5 are used to generate a stable reference voltage for the Wheatstone Bridge. The output of the

Wheatstone Bridge is partially amplified (U1-7), stabilized against long term drift (voltage follower U1-1) and made offset-adjustable by R18 and associated resistors.

Maximum transducer sensitivity can only be achieved when the output is zero volts at TP-1 and when there is no pressure differential across the transducer. To accomplish this, R18 is adjusted for zero volts when both transducer inlet ports (P1 and

P2) are open to atmospheric pressure.

In order to maintain the operating point of comparator U2-14 at the fixed 2-volt trip level, it is necessary to maintain the output of U2-8 within a relatively narrow range. This is accomplished by making the differential amplifier (whose inputs are U1-10 and U2-10) adjustable by selecting 1 of 4 possible jumper positions. A stable reference point for the DC operating level of

U1-8 and U2-8 is established by U1-14 in conjunction with R6 and resistor network RP1.

Measurement of pressure in the range of approximately 0.5 lbs/sq. inch is accomplished by using transducer inlet port P1 in conjunction with jumper setting A/B.

Vacuum pressure in the range of 8 inch Hg is accomplished by using inlet port P2 in conjunction with jumper setting C/D. The regulation point for either vacuum or pressure is established by the setting of potentiometer R16.

When the output of comparator U2-14 goes positive, the collector of Darlington transistor Q2 is pulled to ground, thereby turning on either the pressure or vacuum pump. When the pumps are running, LED DS1 is lit and stays lit until either the pressure or vacuum increases past the hysteresis point established by R8 of U2-14.

The output of the pressure/vacuum regulator can be inhibited by a logic low at J1-1. This completes the circuit description.

Power Supply Assembly

Note

Refer to major subsystem descriptions of AC and DC Power Distribution Subsystem .

Pump Relay Module (PRM)

Refer to

Solenoid, Motor Drive and Pump Block Diagram

The PRM provides drive to the vacuum and pressure pumps, via three Solid State Relays: K1, K2, and K3.

Single Board Computer (SBC)

The Single Board Computer (SBC) is designed around a Celeron 850MHz microprocessor and connects directly into the

Backplane PCB. The SBC receives power from the Power Supply Assembly via the Backplane PCB and receives status and measurement data from the CCM through the DLA. The board contains EPROM, CMOS, RAM, input/output circuitry for the interface ports, disk drives (hard and floppy) circuitry, LCD Display Screen Interface, and PS/2 Keyboard connectivity.

Data Link Adapter (DLA)

The Data Link Adapter provides interfaces from the SBC to both the CCM (Cell Count Module) and the Touch Pad

(membrane) keyboard. These two interfaces function independently under user interface software control. However, some of the circuits on the DLA board are shared. The DLA uses an 82C55 PPI (Parallel Peripheral Interface) IC. Refer to the figure below for an illustration of the DLA board.

DLA (Data Link Adapter) Block Diagram

Interface to CCM

A 20-pin ribbon cable connects the DLA to the CCM. This interface is a bi-directional, parallel interface that is software-controlled at both ends. Data is transferred in 8-bit bytes on 8 data lines (D0 - D7) in one direction at a time. The

SBC (Single Board Computer) initiates an inquiry message handshake on a periodic basis or when it has a command to send.

The CCM responds by sending data or replying to the command sent.

The interface is controlled cooperatively by the UIC and CCM according to the state of the handshake signals. Refer to

Cell

Count Module (CCM)

. The UIC always sends data first. The CCM responds by sending data back (if any is available). Thus, the 82C55 switches its A-port from input to output and back to input during every message transaction.

The key handshake signals are REQ1 and REQ2. REQ2 going low initiates the communication protocol. The CCM responds by bringing REQ1 low. After the DLA has sent its data, it brings REQ2 high. Then the CCM sends its data.

Each byte received by the DLA generates an IRQ. The application software responds to the IRQ by putting the byte into a buffer. When the CCM brings REQ1 high, the communication is complete; the DLA returns to an idle state, and port A is set to input. Both REQ1 and REQ2 remains high until the next message/data transfer.

The two LEDs on the printed circuit board, DS1 and DS2, indicate the active state of REQ2 and REQ1, respectively. They should always be flickering when the application software is running because the User Interface Software program is constantly polling the CCM to check its state. (There are some exceptions to this during power up and certain diagnostics/test modes.)

The I-O address of the DLA board is assigned by four jumpers. The default I-O address (pins 3 - 6 on S1 hard-wired) is: [off

off on off], 340 hexadecimal. The DLA interrupt level is assigned by a jumper.

Jumpers W1 - W6 assign the DLA interrupt to one of the following:

W #

IRQ #

IRQ 5 *

IRQ 10

IRQ 11

IRQ 12

IRQ 15

* Since IRQ 5 is used by the DLA, W1 is hard-wired (this is the default).

Interface to Touch Pad (membrane) Keyboard

A 14-pin ribbon cable connects the DLA to the key panel. The key panel is also polled by the User Interface Software program. This interface is not interrupt-controlled.

The keyboard is interfaced as a parallel switch matrix circuit with 4 lines out and 8 lines in. Four scan rows are driven active one at a time via an LS175 latch. Then the columns of the key panel matrix are read from port B of the 82C55. The software interprets a low active signal as a key is pressed.

The DLA board must be plugged into a 16-bit slot. There are no adjustments on the DLA board.

Troubleshooting

Diagnostics Menu Usage

Utilization of the DIAGNOSTICS Menu enables the operator and/or service representative to identify and correct both operator-correctable and service-correctable faults. When the computer senses a fault, the message <NOT READY: SEE

DIAGNOSTICS> displays in the System Status Box. The following keys are available in the DIAGNOSTICS Menu.

Level One

• INITIALIZATION: Used to perform an Initialization cycle: returns movable components to home position and performs internal self-tests.

• RAW DATA: Used to display raw measurement data for the last specimen.

• COUNT TEST: Used to run specimens without returning to RUN Menu and display Raw Data.

• MORE: Used to display additional functions (levels).

• PRINTER OUTPUT: Used to toggle printer output ON and OFF.

• HELP/ERROR: Used to display help information regarding the diagnostics menu screens. The fault log can also be accessed through this key function.

• MAIN: Used to return to Main Menu.

Level Two

• WBC HISTOGRAM: Used to display WBC count and histogram data accumulated in each of 256 size channels.

• RBC HISTOGRAM: Used to display RBC histogram data accumulated in each of 256 size channels.

• PLT HISTOGRAM: Used to display PLT count and histogram data accumulated in each of 256 size channels.

• SMOOTHING ON/OFF: Used to toggle histogram display status. With Smoothing Off, only raw counts are displayed.

With Smoothing On, channels are numbered, data is normalized and the number of the peak channel displays.

Smoothing On/Off (example)

gives an example of Smoothing On/Off.

Level One

description.

• PRINTER OUTPUT: See

Level One

description.

Level One

description.

• MAIN: Used to return to Main Menu.

Level Three

• PROBE HOME: Moves Sample Probe up and above RBC Cup. Displays probe assembly sensor status.

• PROBE UP: Moves Sample Probe up. Displays probe assembly sensor status.

Level One

description.

• PRINTER OUTPUT: See

Level One

description.

Level One

description.

• MAIN: Used to return to Main Menu.

Level Four

• SYSTEM STATUS: Used to display all pending alarms.

• FAULT REPORT: Used to display all pending faults or warnings.

• SERVICE HEX CODES: Hex system codes. Not used for operator or service troubleshooting.

• SERVICE DEC CODE: Used to initiate individual actions in the CELL-DYN 1800 hardware and software.

Level One

description.

• PRINTER OUTPUT: See

Level One

description.

Level One

description.

• MAIN: Used to return to Main Menu.

Smoothing On/Off (example)

Fault Report Description

A detailed list of all faults generated by the CELL-DYN 1800 System software and hardware is contained in

CELL-DYN 1800

Error Messages

. The fault classifications reported in the Fault Report primarily contains data pertaining to the last CCM fault.

If a fault occurs, pressing the [HELP/ERROR] key immediately displays the [FAULT LOG] in the DIAGNOSTICS Menu. This log may contain up to 16 faults, with the most current fault at the top of the list. An alternative procedure is to go to the MAIN

MENU and press [DIAGNOSTICS]. In this case, the [FAULT REPORT], not the Fault Log, is immediately displayed.

The Fault Log can be viewed from any of the menus, except SETUP. To view the Fault Log, enter the desired menu, followed by [HELP/ERROR] and [FAULT LOG]. The system displays up to 16 past faults. From the MAIN MENU, press

[DIAGNOSTICS] followed by [MORE] three (3) times and [FAULT REPORT] to display the FAULT REPORT screen. A display of <NO FAULTS OR WARNINGS PENDING> indicates that all faults have been cleared.

CELL-DYN 1800 Troubleshooting Guide

A list of symptoms, probable causes, and corrective actions for the most common problems encountered on the

CELL-DYN 1800 System is given in the Troubleshooting Chart. The probable causes and corrective actions for each symptom are arranged in descending order from most likely to least likely. When troubleshooting a problem, start with the most likely cause first.

If possible, thoroughly verify that a component is defective before replacement. Some problems can be verified visually, but other problems may require a measurement tool such as a DVM (Digital Volt Meter).

When troubleshooting DATA PROBLEMS, only the measured parameters RBC, PLT, WBC, HGB, and MCV should be used for reference. Using the calculated parameters can become confusing when trying to isolate a problem.

When troubleshooting CLOG AND FLOW ERROR PROBLEMS, refer to

VP-04 Metering System Timing Adjustments - RBC and WBC

for the MIN and MAX specifications for the RBC and WBC Upper (T1) and Lower (T2) times.

Troubleshooting Charts

Nonfunctional Instrument Problems

Symptom Probable Cause Corrective Action

No Functions. No Fans

Power Cord Check Power Cord

Power Source Check Power Source

No 5VDC Check that 5VDC LED on Card Cage Backplane is On

Note

If off replace Power Supply Assembly.

No Functions. Fans Run

Defective CCM Replace CCM

Defective SBC Replace SBC

Video Display Problems

Symptom Probable Cause Corrective Action

Loose Connection Check all LCD Display Screen Connections

LCD Display Screen

Blank/Solenoids OK

Note

Do not measure voltage on backlight inverter

PCB. Measuring voltage can damage PCB.

Flash BIOS or

CMOS settings reset to Default

Defective Backlight

Interconnect PCB

Replace Backlight Interconnect PCB

Defective LCD

Interconnect PCB

Connect a computer monitor to the top of the SBC PCB. Power ON instrument and open CMOS setup screen. Check that Advanced

Chipset Setup is configured correctly. Refer to

VP-05 CMOS Setup

Verification/Adjustment

Replace LCD Interconnect PCB

Defective LCD

Display Screen

Replace LCD Display Screen

Characters Out Of Focus Defective LCD

Display Screen

Replace LCD Display Screen

Defective SBC

PCB

Replace SBC PCB

Characters OK But Garbled

Defective LCD

Display Screen

Replace LCD Display Screen

Defective SBC

PCB

Replace SBC PCB

Missing Characters

Defective LCD

Display Screen

Replace LCD Display Screen

Displayed Error and Fault Problems

Symptom

Detergent Empty

Probable Cause

Restriction

Defective In-Line Sensor

Defective CDM

Solenoid 3-1, 3-3, 3-4 Tubing

Corrective Action

Check Lines

Check In-Line Sensor

Replace Sensor PCB

Replace CDM

Check Solenoid 3-1, 3-3, 3-4

Diluent Empty

Pressure Overlimit

Vacuum Low Error

Pressure Low Error

Waste Overflow Into

Accumulators

Restriction

Defective In-Line Sensor

Defective CDM

Solenoid 3-3

Solenoid 3-4

Solenoid 3-1

Defective Pressure Switch

Defective CDM

Leak 8" Hg

Defective Pump

Defective Vac Regulator

Leak 0.5 Psi

Defective Pump

Defective Pres Reg

No Air Pressure

Solenoid 5-3

Solenoid 5-7

Solenoid 1-6

Check Lines

Check In-Line Sensor

Replace Sensor PCB

Replace CDM

Check Solenoid 3-3

Replace Solenoid 3-3

Check Solenoid 3-4

Replace Solenoid 3-4

Check Solenoid 3-1

Replace Solenoid 3-1

Check Pressure Switch

Replace Switch

Replace CDM

Check for air leaks in Fluid Power Supply and

Flow Panel

Replace Pump

Replace Vacuum Regulator

Check for air leaks in Fluid Power Supply and

Flow Panel

Replace Pump

Replace Pressure Regulator

Check Unreg Pressure

Replace Unreg Pump

Replace CDM

Check Solenoid 5-3

Replace Solenoid 5-3

Check Solenoid 5-7

Replace Solenoid 5-7

Check Solenoid 1-6

Replace Solenoid 1-6

Waste Empty Timeout

Check Accumulator Sensor Connections

Accumulator Sensor Falsely Detecting

Liquid

Flush Accumulator with DI Water

Replace CDM

Defective Internal Waste Sensor

Check Internal Waste Sensor Connections

Replace Sensor

Unreg Air Pressure Low

Check Unreg Pressure

Replace Pump

Restriction

Defective CDM

DLA/CCM Cable connection

Defective DLA/CCM Cable

CCM/DLA Communication Error

Defective CCM Board

Defective DLA Board

Defective SBC PCB

Power Source

Check tubing at Fluid Power Supply, Waste

Bottles, A and B

Replace CDM

Check DLA/CCM Cable connection

Replace DLA/CCM Cable

Replace CCM Board

Replace DLA Board

Replace SBC PCB

Disk Errors (Hard Disk or Floppy

Drives)

Defective SBC PCB

Check 5VDC and +12VDC (Digital) at Card Cage

Backplane PCB

Check CMOS Setup

Replace SBC PCB

Replace Disk Drive Defective Disk Drive (Hard Disk or

Floppy Drives)

Defective Switch

Defective CDM

Replace Switch and Perform Alignment

Replace CDM

Run Motor Power test

Replace Chopper Drive PCB

Position Faults

Defective Stepper Drive Printed

Circuit Board

Defective Sample Probe Assembly

Defective Motor

Exercise Probe (Diagnostic Menu, Probe Home)

Replace Sample Probe Assembly

Run Motor Power Test

Replace Motor

Data Problems

Symptom Probable Cause Corrective Action

All Results Are "0" No ±12VDC (Analog)

Check ±12VDC (Analog) LEDs on Card Cage Backplane

PCB

Replace Power Supply Module

Replace CCM

HGB OK All Others "0"

No 100VDC

Defective SPM

Defective PAM

Check that PAM 100VDC LED is On

Replace Power Supply Assembly

Replace SPM

Replace PAM

Check for bubbles in Detergent line and Solenoid 2-6

HGB "0" all others OK

Reference lower than sample reading

No sample aspiration Check associated tubing, solenoids and sample syringe

Perform

VP-08 Pre-Amplifier Module (PAM) Adjustment

Defective PAM

Defective CCM

Replace PAM

Replace CCM

Defective HGB Flow Cell Replace HGB Flow Cell

0.5 psi (Bubble Mix) pressure

Check 0.5 psi (Bubble Mix) pressure

Check associated tubing and hardware

Perform

Pressure Adjustment (0.5 psi)

Inadequate Probe Cleaning Check associated wash block tubing and hardware

Imprecision on all Parameters

Imprecise Sample Aspiration

Perform

Sample Volume Verification

Check associated sample aspiration tubing and hardware

Imprecision on all Parameters,

HGB OK

Imprecise Diluent Dispense Perform

Diluent Volume Verification

Carryover Check for correct draining of Pre Mix Cup

Defective SPM

Defective PAM

Replace SPM

Replace PAM

Dirty RBC/PLT Transducer Clean RBC/PLT Transducer

Imprecision on RBC and PLT,

WBC/HGB OK

Incorrect 0.5 psi (Bubble

Mix) Pressure

Defective SPM

Carryover

Dirty WBC Transducer

Imprecision on WBC/HGB,

RBC and PLT OK

Incorrect 0.5 psi (Bubble

Mix) Pressure

Defective SPM

Carryover

Dirty Flow Cell

Reference reading out of

Imprecision on HGB, Others OK specification

Defective Flow Cell

Solenoid operation

Dirty Aperture.

RBC RER

Imprecision on MCV

Defective SPM

Dirty Transducer

Incorrect Lyse Volume

WBC "R" Codes, Reagents OK

WBC Gain

High Electrical Backgrounds

Defective PCBs

Environmental

Check 0.5 psi (Bubble Mix) Pressure

Check associated tubing and hardware

Perform

Pressure Adjustment (0.5 psi)

Replace SPM

Check for correct draining of RBC/PLT Transducer

Clean WBC Transducer

Check 0.5 psi (Bubble Mix) Pressure

Check associated tubing and hardware

Perform

Pressure Adjustment (0.5 psi)

Replace SPM

Check for correct draining of WBC Transducer

Clean Flow Cell

Check reference reading (Diagnostics Menu, Raw Data)

Perform HGB Ref Adjustment on PAM

VP-08 Pre-Amplifier

Module (PAM) Adjustment

Replace Flow Cell

Check Solenoid 3-6, 2-6 and 2-7

Clean RBC/PLT Transducer and Aperture Plate

Perform

VP-09 Signal Processor Module (SPM)

Verification/Adjustment

Replace SPM

Clean WBC Transducer and Aperture Plate

Replace Lyse Syringe

Replace Lyse Syringe Drive Assembly

Perform

VP-09 Signal Processor Module (SPM)

Verification/Adjustment

Replace SPM

Replace CCM

Check power

Check overhead lighting

Check for other items on same power line

Clog and Flow Error Problems

Poor instrument cover grounding

Install filter (line conditioner)

Isolate line (dedicated line)

Check grounding cable on Front Cover

Replace grounding cable

Check EMI Shielding on Card Cage Backplane PCB

Defective PAM

Check correct routing of WBC and RBC/PLT Transducer cables to PAM

"Noisy" Power Supply

Module

Replace PAM

Check Power Supply Module

Replace Power Supply Module

Check Connections on Pre-Amplifier Filter PCB

Defective Pre-Amplifier Filter

PCB

Replace Pre-Amplifier Filter PCB

Defective Transducer(s) Replace Transducer(s)

"CLOG" T1 = Max T2 = 0, No

Meniscus

Symptom

"CLOG" both sides constant

"CLOG" T1 = Max T2 = 0, No Vent

Probable Cause

Incorrect vacuum

Corrective Action

Perform

VP-03 Vacuum and Pressure Adjustments

Check Solenoid 1-4 and 1-5 (closed during metering)

Diluent and Detergent lines reversed

Check Reagent lines

Metering Tube position Top of Metering Tube (tapered edge) should be visible on top of upper DET sensor

Restriction

Dirty Transducer

Restriction

Check Vent Tubing

Check Vent Solenoid 3-6, 2-1 and 1-3

Clean Transducer and Aperture Plate

Check plumbing

Check Count Solenoid 1-2 and 4-3

Check Vent Solenoid 1-3 and 2-1

"CLOG" T1 = Max T2 = 0, Slow

Meniscus

Dirty Transducer

Restriction

Check for bubbles on right side of Transducers

Clean Transducer and Aperture Plate

Check associated tubing

Check Vent Solenoid 3-6

"CLOG" T1 = Max Meniscus speed OK

Incorrect Vacuum

Defective Upper Detector

Defective CDM

Perform

VP-03 Vacuum and Pressure Adjustments

Check Solenoid 1-4 and 1-5 (closed during metering)

Replace Metering Printed Circuit Board

Replace CDM

"CLOG" T1 =OK T2 = Max

Meniscus speed OK

Defective Lower Detector

Defective CDM

Replace Metering Printed Circuit Board

Replace CDM

"FLOW ERR" T1 = Min T2 = Max

Defective Upper Detector Replace Metering Printed Circuit Board

Defective CDM Replace CDM

"FLOW ERR" T1 = OK T2 = Min

Defective Lower Detector Replace Metering Printed Circuit Board

Defective CDM Replace CDM

Miscellaneous Problems

Symptom Probable Cause Corrective Action

Defective SPM Replace SPM

Garbled Histograms

Defective CCM Replace CCM

Raw Data Description

From the MAIN MENU, press [DIAGNOSTICS] followed by [RAW DATA]. The [RAW DATA] key displays raw data obtained from the last count cycle.

When a single count is done, all data is contained in the first column. When a PLT recount occurs, data from the first cycle displays in column #2 and data from the recount displays in column #1.

Raw Data Display Description

• RBC, WBC and PLT counts are RAW, uncorrected total counts.

• HGB Error is not used.

• HGB Reference is the output of the A/D Converter when reading reference (2000 = 5 volts).

• HGB Sample is the output of the A/D Converter when reading sample (2000 = 5 volts).

• WBC and RBC Up Times are the upper times in milliseconds for the last sample.

• WBC and RBC Count Times are the times in milliseconds for the last sample.

• Flow Error is coded Clog or Flow Error data.

• RBC RER is RBC Cell Editing percentage.

• WBC and RBC Upper max and Upper min are the maximum and minimum Upper Times, respectively.

• WBC and RBC Avg. Time are the averages of the previous count times. The WBC and RBC Avg. time is reset when the [Clear Orifice] key is pressed.

• WBC and RBC Time-Outs are the floating Upper Clog Alarm Limits calculated by the "Running Average Program".

Note

An example of a raw data report displays in Raw Data (example) .

Raw Data (example)

CCM On-Board Diagnostic LEDs

The seven LEDs on the CELL-DYN 1800 CCM can reveal much about the fundamental CCM and overall machine state. In general, the LEDs indicate whether the CCM is in a normal functioning mode or in a fault state, and in either case, help to characterize the CCM state. Also, LED2 gives some information about the state of the SBC.

The CCM tests itself on power-up. These fundamental tests include MC68HC11KW1, RAM, and SPM interfaces. If any test fails, the CCM attempts to execute an endless loop routine which flashes the green LED on the board. Also, it places a 4-bit fault code into the adjacent yellow LEDs (see

Power-on LED Patterns - Fault States on Startup

LED Function & Counting Control

The LEDs on the CCM (labeled LED1 through LED7) are entirely under program control. Their use is as follows:

Note

Refer to CELL-DYN 1800 CCM for location of LEDs on PCB.

• LED7, a green LED, should not be not flashing after the CCM has successfully completed its internal power-on self-check diagnostics, otherwise if it is flashing slowly (~1Hz.), there is a fundamental CCM fault.

• LED5 and LED6, for CER and CEW, indicate the state of the CCM firmware generated signals CER (Count Enable

Red) and CEW (Count Enable White). These signals enable cell counting. When the associated LED is on, the DMA cell counting circuitry is active.

• LED3 and LED4, for WCP and RCP, are on during the metered count time intervals, LED3 for the white count time interval and LED4 for the red count time interval.

• LED2 is driven by the signal NREQ1, and indicates the UIC/CCM communication activity. This LED state directly relates to the hi/lo state of NREQ1. When LED2 is flickering, which should always be the case during normal system operation, it indicates that the UIC/CCM communications link is active. (There is a corresponding LED on the DLA

(UIC comm. board) that will also be flickering in sync with LED2; it is controlled by the DLA output signal NREQ2.)

• LED1 is used to indicate that a self-test is in progress. This indicates that the tests for the pulse processing A to D circuitry on the SPM with the pulse counting circuitry on the CCM are active.

Power-on LED Patterns - Normal Setup

LED2

Step

NREQ1

LED3 LED4

WCP RCP

0 ON ON ON

LED5

CEW

LED6

CER

LED7

READY

ON Power on

State

4 ON ON

5 Flashing ON ON

6 Flashing

Flashing

RAM testing

Flashing RBC/PLT testing

WBC testing

CCM tests done; Homing flowscript running

UIC program comm. started

Initialized (running/idle)

Power-on LED Patterns - Fault States on Startup

LED2 LED3 LED4 LED5 LED6 LED7 CCM

NREQ1 WCP RCP CEW CER

ON ON

READY

Flashing

Fault

MC68HC11KW1 CONFIG reg

MC68HC11KW1 RAM test

ON Flashing 8K RAM bit test

Flashing 8K RAM clear test

ON ON ON Flashing Histogram RAM test

ON ON Flashing MC68HC11KW1 timer test

ON Flashing CDM init. test

CELL-DYN 1800 CCM

1 Cell Count Monitor (CCM) PCB

2 S1 RESET

3 S3 BUFFALLO

4 J2 MPM

5 J1 CDM

6 J3 SPM

7 J5 DLA

CPU Hardware/Software Configuration

RS-232 Communications Test Procedure

8 J6 - NOT USED

9 LEDs 2-7

10 E2 DGND

11 J8 - NOT USED

12 E3 DGND

13 E1 DGND

14 LED 1

Detailed information on the CELL-DYN 1800 System Interface Specifications is available on the WWCS Intranet website.

CMOS Setup

The CMOS Setup contains all the information needed by the Basic Input/Output System (BIOS) to establish proper communications between the single board computer (SBC) and the various computer system devices. Refer to

VP-05 CMOS

Setup Verification/Adjustment

Special Function

Probe Check

There are two probe check functions activated by softkeys in the DIAGNOSTICS menu.

DOWN] softkey then displays to restore the probe to the down position.) displays to restore the probe to the down position.)

Note

Neither procedure puts the probe in the STANDBY position (on the left).

Service Special Commands

Discussion

Several commands are available to initiate individual actions in the CELL-DYN 1800 System hardware and software. These commands are used for troubleshooting and/or alignment when a single action is desired or required to be repeated several times.

The special command mode resides in the DIAGNOSTICS Menu. From the MAIN Menu, press [DIAGNOSTICS] followed by

[MORE] three (3) times, then [SERVICE DEC CODE]. When this softkey is pressed, the message Test Select --- FOR

SERVICE USE ONLY --- displays.

A command can now be entered. Pressing the Enter key on the keyboard initiates the action. Only one command can be entered at a time and [SERVICE DEC CODE] must be pressed before a command is entered.

All commands available by direct softkey can be accessed by pressing [MORE].

Note

Use only the commands listed in DEC Service Commands and always verify that the correct number has been entered before initiating the action. Use only those numbers listed in DEC Service Commands . Other numbers may refer to engineering commands which are not used in the field and which may cause damage if used improperly. Be fully aware of the purpose of any of the DEC Service Commands before using them. This is a direct-activation method which should be used with caution because the physical state of the CELL-DYN 1800 System may not be in agreement with the function to be performed. After using service commands, always re-initialize the system by turning the power OFF then ON again or by pressing the [INITIALIZATION] key in the DIAGNOSTICS Menu to ensure the instrument is in the proper configuration for normal operation.

DIAGNOSTICS Menu Service Code Function List

When the [SERVICE DEC CODE] key is pressed, the (Enter number (currently, 102):____ prompt displays.

The number above corresponds to the decimal code for the last code entered.

DEC Service Commands

lists the decimal-coded (DEC) service commands that can be invoked by pressing the [SERVICE

DEC CODE] key in the DIAGNOSTICS Menu and entering the appropriate number.

DEC Service Commands

UIC DEC Codes

07 NOT USED

NOT USED

NOT USED fill lyse into system

NOT USED fill Diluent & detergent mini-wash

NOT USED

NOT USED pre-dilute sample run setup pre-dilute sample run exit

Function

47 aperture current off (uses whole blood script) open all valves

NOT USED platelet recount initialization (homing) open sample run clean orifice (back-flushing) pre-dilute sample run background count run prime system with all reagents daily shutdown empty transducers and cups gain adjust unpinching normally closed valves fill transducers and cups after empty gain adjustment setup dispense 10 ml saline open sample wash clean-for-shipping clean sample syringe setup aspirate 40 µl sample for 1/250 dilution dispense 10 ml saline for 1/250 dilution aspirate 100 µl sample for 1/50 dilution dispense 5 ml for 1/50 dilution

NOT USED lyse syringe down

NOT USED

NOT USED lyse syringe up and home lyse syringe down restore

123

124

125

126

119

120

121

122

117

118

127

128

129

130

81 pre-dilute sample wash

NOT USED

NOT USED diluent syringe down enzyme clean setup probe up and rotate and home back to ready position from probe home probe up for probe adjustment probe down (when finished, operator should initialize the instrument to place the probe in the home position) sample syringe up and restore sample syringe down and home enzyme clean the system diluent syringe up and home diluent syringe down and restore

NOT USED

NOT USED cycle solenoids on waste assy cycle solenoids on flow panel assy sample syringe aspirate sample syringe dispense vacuum test check mixing pressure check backflush pump motor power test (see

Service DEC Code 128

) motor power level test (see

Service DEC Code 129

) exercise motors (see

Service DEC Code 130

)

999

Note

auto-cycle (see

Auto-Cycling (Code 999)

)

Certain commands are not sent to the CCM when the system is in an interlock state, such as STANDBY or

UNINITIALIZED.

Auto-Cycling (Code 999)

The CELL-DYN 1800 can be pre-set to do a specified number of RUN cycles without user intervention. This capability applies only to normal RUN Count Test, Pre-Dilute RUN, (PRE-DIL TEST), Gain Adjust (GAIN ADJ), and Electrical

Background (ELEC BKGD). This capability helps reduce test time for the instrument. The following entry screen displays after entering code 999:

-- Auto Cycle Test Set Up --

Use Spacebar to accept current number

Use "<--" key to delete a digit

Use "ESC" key to cancel

Enter Number of Times to Repeat Test (currently, 10):

Sample Probe Description

The motors that enable the Sample Probe to move up/down and to rotate are stepper motors which are under direct computer control. Since there is no direct positional feedback sent to the computer, position switches are employed to verify critical positions during normal operation. It is important to understand that these switches only verify and do not control the movement of the Sample Probe.

In the DIAGNOSTICS Menu, Service DEC Codes 128, 129, and 130 allow the service representative to control and exercise all stepper motors in the CELL-DYN 1800 System. This description focuses on the Probe Up/Down Motor (B/2) and the

Probe Rotate Motor (C/3) which control the movement of the Sample Probe.

Service DEC Codes 128, 129, and 130 Descriptions

These commands reside in the SERVICE DEC CODE screen of the DIAGNOSTICS Menu and are used to test, control, and exercise CELL-DYN 1800 stepper motors. A description of each of these three commands is given.

Service DEC Code 128

This code runs a computer generated test (Motor Power Test) of all stepper motors, motor driver boards, and associated circuitry.

The Motor Power Test should be run whenever a problem is suspected with any assembly that is driven by a stepper motor.

The following entry screen displays after entering code 128:

Motor Power Test Started.

To MPM: {I }

To MPM: {pD32}

To MPM: {mC1!2} AC}

To MPM: {C1} inp: 0415

A report (

Motor Power Test (example)

) automatically displays and can be printed. Refer to

VP-11 Stepper Motor Power Test and Verification

Note

Press the [INITIALIZE] key before leaving the DIAGNOSTICS Menu.

Motor Power Test (example)

Service DEC Code 129

This code allows the Run and Idle power levels to be set when exercising a stepper motor. The four levels are:

0) Full Power

1) Medium Power

2) Low Power

3) Off

This code tests mechanical assemblies at various power levels or to remove idle power so the mechanism can be more easily moved or checked manually. The

Motor Power Level Test (example)

screen displays after entering code 129 (press the ENTER key after each entry):

Motor Power Level Test (example)

Note

After the entries are made, a message, such as Motor "A" set to running power of 1 and idle power of 3, displays.

Service DEC Code 130

This code allows the direction, speed, and number of steps to be set when exercising a stepper motor.

The

Motor Check (example)

screen displays after entering code 130:

Motor Check (example)

Note

After the entries are made, a message, such as Motor "B": motion in direction "0" at speed "&" for 100 steps, displays.

Motor Direction Commands

The table below contains information on the motor designation, command and direction of the motor to be tested.

Motor

Speed Commands

lists the motor speed commands to determine the speed of the motor being tested. Both tables are needed to properly test the motor.

Motor Direction Commands

Motor Designations

A/1

Function

Sample Syringe

Command Direction

0 Down/Aspirate

1 Up/Dispense

B/2 Probe Up/Down

0 Up

1 Down

C/3

D/4

Probe Rotation

Diluent Syringe

0 CCW/To RBC cup

1 CW/To Pre-Mixing Cup

0 Down/Aspirate

1 Up/Dispense

0 CCW/Dispense

E/5

F/6

G/7

H/8

Directional Valve 1 CW/Aspirate

Spare

Lyse Syringe

0 Down/Aspirate

1 Up/Dispense

Motor Speed Commands

Command Speed in Steps per Second

1 50

283

300

166

200

250

151

222

182

100

125

111

Sample Probe Normal Operation

Probe Up/Down INITILIZE and RUN Modes

illustrates the Sample Probe's up/down sequence during the INITIALIZE and

RUN cycles.

Probe Rotate "INITALIZE" Mode

shows the probe's rotation movement during the INITIALIZE cycle.

Probe Up/Down INITILIZE and RUN Modes

Probe Rotate "INITALIZE" Mode

Initialization Mode

The Initialization cycle places mechanical and electrical components in the "home" position, drains any liquid in the tubing,

Pre-Mix Cup, and the Mixing Chamber of the von Behrens RBC Transducer to the Waste System, then places the instrument in the INITIALIZED state.

Stepper Motor Homing

Homing a stepper motor is the process of setting up the initial position from which all future movement is referenced. In the

CELL-DYN 1800 System, this is accomplished by forcing the motor to move against a physical stop (Hard Stop). When the mechanical assembly, driven by the motor, reaches the Hard Stop, the stepper motor electrically slips until it is forced to stop.

This mechanical position then becomes the zero reference position for the motor.

Operation:

1. The Sample Probe moves up at a fast speed until the Upper Switch (#2) is activated. It is then changed to a slow speed, and homed against the Upper Hard Stop, which is the metal plate at the top of the Sample Probe Assembly.

2. The probe moves down six steps and the Upper Switch (#2) is checked.

3. The probe moves CCW at a fast speed until the Right Switch (#4) is activated. It is then changed to a slow speed, and homed against the Right Hard Stop, which is the mounting bracket for Right Switch (#4).

4. The probe moves CW to the Pre-Mix Cup and Left Switch (#3) is checked. The probe then moves into the Pre-Mixing

Cup.

5. The probe moves up and Upper Switch (#2) is checked.

6. The probe moves CCW to center and down positions; and the Lower Switch (#1) is checked.

This completes the Initialization cycle.

Run Mode

The figure below illustrates the probe's movements during the RUN cycle.

Probe Rotate "RUN" Mode

Operation:

1. When the Start Switch is pressed, 30 µL of sample is aspirated and Lower Switch (#1) is checked.

2. The Sample Probe then moves up to a position six steps from Upper Hard Stop, and Upper Switch (#2) is checked.

3. The probe moves CW to Pre-Mix Cup and Left Switch (#3) is checked.

4. The probe moves down eight steps and into the Pre-Mix Cup, where dispense, probe shake, and aspiration of RBC sample takes place.

5. The probe then moves up to a position six steps from Upper Hard Stop, and Upper Switch (#2) is checked.

6. The probe moves CCW to the Mixing Chamber of the von Behrens RBC/PLT Transducer, stops three steps from

Right Hard Stop, and Right Switch (#4) is checked.

7. The probe moves down into the RBC/PLT Mixing Chamber and RBC sample is dispensed.

8. The probe moves up to a position six steps from Upper Hard Stop, and Upper Switch (#2) is checked.

9. After completion of the count cycle, the probe moves CW to center position.

10. The probe moves down and Lower Switch (#1) is checked.

11.

This completes the RUN cycle.

Switch Failure Descriptions

Example of fault reports are shown in the following figures:

Lower Switch (#1) Fault Report

Upper Switch (#2) Fault Report

Left Switch (#3) Fault Report

Right Switch (#4) Fault Report

When a switch is checked by the computer and found to be deactivated (open) in normal operation, the message "Not Ready:

SEE DIAGNOSTICS" displays on the RUN Menu.

From the MAIN MENU, press [DIAGNOSTICS]. The screen immediately displays one of the Fault Reports shown in

Lower

Switch (#1) Fault Report

Upper Switch (#2) Fault Report

Left Switch (#3) Fault Report

, and

Right Switch (#4) Fault Report

The message <SWITCH: 1 CHECK> indicates that Lower Switch (#1) failed when checked. The message <* NOT ON ANY

SWITCH *> indicates that none of the switches were activated when the failure occurred. Refer to

Lower Switch (#1) Fault

Report

Lower Switch (#1) Fault Report

The message <SWITCH: 2 CHECK> indicates that Upper Switch (#2) failed when checked. The message <* NOT ON ANY

SWITCH *> indicates that none of the switches were activated when the failure occurred. Refer to

Upper Switch (#2) Fault

Report

Upper Switch (#2) Fault Report

The message <SWITCH: 3 CHECK> indicates that Left Switch (#3) failed when checked. The message <ON SWITCH(ES):

2 TOP> indicates that Left Switch (#3) was activated when the failure occurred. Refer to

Left Switch (#3) Fault Report

Left Switch (#3) Fault Report

The message <SWITCH: 4 CHECK> indicates that Right Switch (#4) failed when checked. The message <ON SWITCH(ES):

2 TOP> indicates Right Switch (#4) was activated when the failure occurred. Refer to

Right Switch (#4) Fault Report

Right Switch (#4) Fault Report

The above conditions do not necessarily indicate that a switch has actually failed. They only indicate that the switch was not read as activated when checked by the computer. A failure could also be caused by improper switch alignment, an electronic hardware failure, or a mechanical hardware failure.

CELL-DYN 1800 Error Messages

The table below lists error messages on the CELL-DYN 1800 System.

Error Message

(Status box)

Time-out at N seconds

Description

Process Aborted

A CCM process initiated by the user took longer to complete than allowed (usually indicating a failure of the CCM). The process ran approximately N seconds before the time-out occurred.

A count test was stopped either by the user or because of a fault detected by the CCM.

A user-correctable fault condition was detected.

Fix then press [CLEAR

ALARM]

Process Monitoring Aborted A process was stopped by the user (using the asterisk (*) key.

Error Message

(Display Area II)

Description of the error.

Printer Time-out The Printer Output option was ON and the printer did not print the requested report in the

Code N is Invalid

Cannot do this Function

WBC Meniscus Detection,

RBC Meniscus Detection

WBC Count Time-out (clog),

RBC Count Time-out (clog) expected time.

The user has entered a command for the CCM whose numeric value exceeds 127. The value entered was N.

The user has attempted to issue a command to the CCM that cannot be executed because of a pending fault condition.

During the most recent count, a meniscus was not detected or was detected at an unexpected time.

During the most recent count, a Clog occurred.

CCM Pulse Height Memory

Saturation Warning

External Waste Full

During the most recent count, there was an overflow in one of the pulse-height arrays

(histograms).

The external waste bottle has been filled.

The indicated reagent has run out.

Lyse Empty, Detergent

Empty, Diluent Empty

Invalid Alarm Set

*NOT ON ANY SWITCH*

A bit was set in the fault message from the CCM that has no valid interpretation.

After some mechanical motion, a reading of all the position sensors indicates that none are activated. (This message does not necessarily mean that a mechanical fault has occurred.)

Waste Overflow Into

Accumulators

Error Message (Status box) Description

Vacuum There was a vacuum failure during power-up or the instrument is unable to maintain vacuum level while in the READY state.

Pressure

Position Fault

A reading of the sensor in the Waste Accumulator suggests that there is liquid in the accumulator.

There was a pressure failure during power-up.

A mechanical assembly is not in the correct position for the most recent function to be performed, as indicated by position sensors.

Sensor Fault - Internal Waste

Empty

A Time-Out fault occurred in draining one of the waste bottles. This error is also associated with positive pressure.

Canceling Auto-Cycling This message displays when the user cancels Auto-Count Testing.

Invalid UIC Command Sent to

CCM

The UIC sent a command to the CCM that it cannot interpret.

Error in Flow System Timing An error in the timing of a flow script has occurred. This occurs during instrument initialization when the flow script takes more than three minutes to complete.

Histogram Memory Clear The CCM was unable to clear the pulse-height memory.

CCM Program, RAM Memory The CCM detected a failure in its RAM.

CCM/MPM Message Fault

Other error-related messages

MPM to CCM, Message

Transmit Error

Command to be Sent to MPM is Incorrect

Abnormal Time-out/No MPM

Response

An error in CCM/MPM interprocessor communications occurred. A fault was generated in an attempt to send or receive motor or other command to or from MPM, or the MPM was unable to perform the function.

Fault Response from MPM

Incorrect Command to be

Sent to MPM

Attempt to Send MPM a New

Command while Busy

No Such Script in ROM or

RAM

CCM/UIC Message Fault

No Response from CCM

CCM is Initializing

Undefined Event

Error in loading a flow script.

An error in UIC/CCM interprocessor communications occurred.

The CCM is not functioning or the signal cable connecting the CCM and UIC is faulty or disconnected. Turn the instrument OFF, check the CCM/UIC cable, then turn the instrument

ON.

The CCM is in the middle of its Initialization process.

An undefined event or process occurred.

Count Test

The [COUNT TEST] key in the DIAGNOSTICS Menu is used to run specimens and display Count Check data without returning to the RUN Menu. Coded data relating to specific cycle functions, raw measurement, and flow count time are displayed for use in troubleshooting or service.

Event Messages During Diagnostic Menu Count Test

lists the event messages that are displayed during the Diagnostic Menu

Count Test.

Event Messages During Diagnostic Menu Count Test

Event Messages Description

SampSw. pressed Touch Plate was pressed.

Remove specimen Specimen should be removed.

Count valve open The counting valve is open.

RBC histogram avail RBC histogram is available.

WBC upper det

WBC lower det

WBC upper meniscus detection.

WBC lower meniscus detection.

RBC upper det

RBC lower det

Plt recount strt

CCM initing

Data invalid

Data avail

CCM init done

Canceled

RBC upper meniscus detection.

RBC lower meniscus detection.

Platelet recount starts.

CCM initializing.

Data entered is invalid.

Data is available.

WBC histo avail WBC histogram is available.

Proc complete Process is completed.

CCM initialization is completed.

Canceled operation.

Operator-Correctable Alarm or Fault Messages

This table lists operator-correctable alarm or fault messages.

Event Messages Description

External Waste Full Waste full sensor is activated.

Detergent Low Detergent is low as detected by reagent sensor in reagent inlet tube.

Diluent Low

Lyse Empty

Diluent is low as detected by reagent sensor in reagent inlet tube.

No lyse is detected by reagent sensor in reagent inlet tube.

Invalid alarm set Incorrect error or message was set.

Detergent Empty No detergent is detected by reagent sensor in reagent inlet tube.

Diluent Empty No diluent is detected by reagent sensor in reagent inlet tube.

CCM is initializing CCM module is currently in the INITIALIZED state.

Summary of Error Messages

Summary of Error Messages

gives a complete listing of error messages available on the CELL-DYN 1800 System.

Summary of Error Messages

Error Message (Status box)

DOS Errors

Arithmetic overflow

Bad drive request structure length

Cannot remove current directory

Cannot rename across drives

Collection index out of range

Collection overflow

CRC error in data

Device read fault

Device write fault

Disk full

Disk is write-protected

Disk read

Disk seek

Division by zero

Drive not ready

File access denied

File not assigned

File not found

File not open

File not open for input

File not open for output

Floating point overflow

Floating point underflow

Hardware failure

Heap overflow

Invalid drive number

Invalid file access code

Invalid file handle

Invalid floating point operation

Invalid function number

Invalid numeric format

Invalid pointer operation

Path not found

Printer out of paper

Range check

Sector not found

Stack overflow

Too many open files

Range check

Sector not found

Stack overflow

Too many open files

Unknown command

Unknown media type

Unknown unit

Demographic entry disabled

No data to print

General

Stopping

Unable to Load Flowscript

Unable to Save Cal Factors

Unable to Set Volume

Not Ready: See DIAGNOSTICS

Uninitialized

Detergent empty

Diluent empty

Lyse empty

Waste full

<-? Invalid error code

CCM command echoed does not match

CCM REQ1 high during data send at char

CCM REQ1 is stuck LOW

DLA buffer overflow on receiving char

DLA/CCM error on command

Incorrect checksum on CCM data receive

NAK received from CCM

No response from CCM

Time-out 1 on wait for CCM REQ1

Time-out 2 on message send to CCM

Time-out 3 on message receive from CCM

System Fault

DLA Faults

Normal Operator-Correctable

Date

Battery failure or system date/time not set

Cannot open CD1800.ini configuration file

Cannot read CD1800.ini configuration file

Cannot write to CD1800.ini configuration file

CD1800.ini configuration file size error

CD1800.ini configuration file version error

Configuration file error

Error when reading CRC values from disk

Memory error when creating CRC tables

Data err

Inc. asp.

No Ticket Detected

Can not accept specimen

Can not reject specimen

No loading, QC file has to be empty

Purge log failed

Read QC file failed

Cannot do if uninitialized

Cannot write Data Log header to disk

Count overrange

Data Log write error

Failed to read from Data Log

Disk I/O

Ticket Printer

QC Log

Data Log

Flow err

Data Log initialization failed

QC Log initialization failed

Break interrupt

Comm open error

Comm error

Framing error

Parity error

Re-transmit started

Re-transmit time-out

Receiver overrun

Transmit started

Unable to re-transmit

Printer Fault

Printer Not Ready

Printer Off-line

Printer Out Of Paper

Printer Time-out

Ticket Printer Not Ready

Failure to read from QC Log

Failure to write to QC Log

QC Log size error

Initialize

Communications

Printer Driver

QC Log I/O

QC Log version error, bytes short for creation of QC Log

Help

Unable to open help file

Reagent Log

Unable to create file

Unable to open file

Data Log Errors

Cannot Access Data Log

CRC Reading Failed

Data Log initialize failed, bytes short for creation of Data Log

Data Log Print Error

Data Log size error, Call Technical Support

Data Log version error

Data Transmission Error

Not enough memory error

X-B File

Failed to write to Data Log Header

Failed to write to Data Log

No response from CCM

Data Log I/O

Data Log write failure

Write Header failure

Write version error

General Faults

Abnormal time-out / no MPM response

Attempt to send MPM a new command while busy

CCM pulse height memory saturation warning

CCM is in Fault State

CCM is in Unknown State

CCM Program, RAM Memory Fault

CCM real time clock has failed

CCM/DLA Message Communication Fault

Command to be sent to MPM is incorrect

Count Overrange

Count time-out (clog)

Data Capture Fault

Detected Probe Assembly Switch: # in Incorrect State

Detergent sensor

Diluent sensor

Error in Flow System Timing

Fault response from MPM

Guard electrode voltage warning

Histogram Memory Clear Failure

Incomplete Aspiration

Incorrect command to be sent to MPM

Initial Communication with DLA Failed

Invalid Command Sent to CCM

Invalid Data

Mechanical Position Fault

MPM to CCM, message transmit error

No response from MPM

Press [INITIALIZATION] key to clear fault and re-initialize meniscus detector: "True" sensed at metering start meniscus: not detected during valid time interval

Pressure Level Time-out

Pressure Over-Limit Detected

Printer is not Ready: Cannot RUN uninitialized

RBC Clog

RBC Flow Error

Run time error: # at:

Time out after approximately __ minutes

Turn off instrument and drain accumulators manually

Unexpected response from MPM

Unknown MPM/CCM fault

Vacuum Level Time-out

Waste Drain to Empty Time-out

Waste Overflow into accumulators

WBC Clog

WBC Flow Error

Wrong software for this instrument

Fault Log

Error in writing to Fault Log file

Error in writing header to Fault Log file

Fault Log I/O

Allocating heap memory error

Bytes short to create the Fault Log

Fault Log file size error

Fault Log Header IO Error

Incorrect version of Fault Log

Lab ID Setup

Drive A is not ready

Fail to read assay file

Fail to write LAB ID file

Incorrect assay format

Incorrect disk. QC values do not apply to this instrument

Incorrect Exp. Date

Incorrect Lot Number

Incorrect parameter in assay file

Software Commands and Sequence

CD1800 File and Directory Structure

Accessing DOS

There are two ways to access the DOS program from the CD1800 program:

• With the CD1800 program still loaded,

• By exiting the CD1800 to DOS. The

Exiting from CD1800 to DOS

method is the preferred method.

Switching from CD1800 to DOS

1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.

Press ESC on the PC keyboard. The following message displays on the screen.

CD1800 Program Exit

It is recommended that the Cell-Dyn be put in Stand-by before exiting the program. Otherwise, turn the instrument off after doing any service work while in DOS.

Alt and d keys simultaneously. The DOS prompt C:\CD1800> displays.

Execute the desired DOS commands. Most commands can be executed. However, computer memory is limited since the CD1800 program is still loaded.

Exiting from CD1800 to DOS

1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.

Press ESC on the PC keyboard. The following message displays on the screen.

CD1800 Program Exit

It is recommended that the Cell-Dyn be put in Stand-by before exiting the program. Otherwise, turn the instrument off after doing any service work while in DOS.

prompt (C:\1800>) displays.

Execute the desired DOS commands.

Exiting DOS

To exit the DOS program and return to the CD1800 program in the case where the CD1800 program remains loaded

(Alt and d was used to access DOS), type exit at the C:\> prompt and press the Enter key. The DIAGNOSTICS Menu displays.

To exit the DOS program in the case where the CD1800 program is not loaded, (Alt and x was used to exit the CD1800 program), turn the instrument OFF then ON again to reinitialize the system.

Note

Turning the instrument OFF then ON again prepares the system for normal operation.

Common DOS Commands

Function Command Description

Change drives c: or a: switch C and A drives

Change directory cd filename

Copy copy source to destination

Delete

Engineering Drawings and Schematics

见 CD1800 电路图

CELL-DYN 1800 PCB Reference

AC Regulator PCB

1 AC Regulator PCB

2 J2

3 TP6

4 TP7 (+14V)

5 TP5 (VR)

6 TP4 (GND)

7 R22

8 J4

9 J7

10 TP9 (GND)

11 TP8 (+100V)

12 R28

13 J9

14 J1

15 J3

16 J8

17 LD3 (+14V)

18 LD4 (+5V)

19 J5

20 LD1 (+12V)

21 LD2 (-12V)

22 TP2 (GND)

23 TP3 (+12V)

24 TP1 (-12V)

25 J6

Backlight PCB

1 Backlight PCB

2 CN1

Backplane PCB (front)

3 CN2

1 Backplane PCB

2 J2

3 SBC

4 DLA

7 LD4 (+12V Digital)

8 LD5 (-12V Digital)

9 LD2 (+12V Analog)

10 LD3 (-12V Analog)

5 CCM

6 SPM

Backplane PCB (rear)

11 LD1 (+5V)

1 Backplane PCB

2 J1

3 TP6 (DGND)

4 TP1 (+5V)

5 TP5 (-12V Digital)

6 TP4 (+12V Digital)

CCM PCB

7 TP3 (-12V Analog)

8 TP2 (+12V Analog)

9 J10

10 J9

11 J11

1 CCM PCB

2 S1 (RESET)

3 S3 (BUFALLO)

4 J2 (MPM)

5 J1 (CDM)

6 J3 (SPM)

7 J5 (DLA)

CDM PCB

LEDs 2-7

10 E2 (DGND)

11 J8

12 E3 (DGND)

13 E1 (DGND)

14 LED1

J17

J18

J19

J32

J20

J13

J14

J15

J16

CDM PCB

TP1 (GND)

J11

J12

J29

J30

J31

J10

J25

J26

J27

J28

DC Regulator PCB

J21

J22

J23

J24

1 DC Regulator PCB

2 LD2 (+14V)

3 J5

4 J7

5 J6

6 J4

7 J2

8 J3

DLA PCB

9 J1

10 J11

11 LD3 (+12V)

12 J8

13 J9

14 J10

15 LD1 (+5V)

1 DLA PCB

2 J1

3 TP1

4 DS1 LED

Indicator PCB

5 DS2 LED

6 J2

7 TP2

1 Indicator PCB

2 LD1 (green)

Internal Waste Empty PCB

3 LD2 (yellow)

4 LD3 (red)

1 Internal Waste Empty PCB

2 E2

3 E1

LCD Interconnect PCB

4 J1

5 J2

1 LCD Interconnect PCB 6 J2

2 J8 7 J3

3 J7

4 J5

5 J6

JP1B

JP1A

J4 (USED FOR CELL-DYN 1800)

MPM PCB

1 MPM PCB 7 TP4 (+28V)

2 DS1 thru DS4 LED'S

3 TP1 (GND)

4 TP3 (RESET)

5 J3 thru J14

6 TP7 (+5V)

PAM PCB

8 TP2 (GND)

9 TP6 (+12V)

10 J2

11 J1

PAM PCB

JMP1

13 TP3 (GND)

14 JMP3

15 R14

16 J5

TP7

TP1

9 JMP2

10 TP2

11 J2

12 TP8 (GND)

Pre-Amp Filter PCB

17 R72

18 LD3

19 TP5 (GND)

20 R66

21 TP6 (GND)

22 LD1 (-12V)

23 LD2 (+12V)

24 TP4 (+100V)

1 Pre-Amp Filter PCB 4 -12V

2 +12V

3 GND

PRM PCB

5 J1

1 PRM PCB

2 J5

3 J8

4 J7

5 J6

RBC Metering PCB

6 J1

7 J2

8 J3

9 J4

10 F1

1 RBC Metering PCB

2 J1

3 TP1

Reagent Empty Sensor PCB

4 TP2

5 TP3

1 Reagent Empty Sensor PCB

2 E1

3 E2

4 E3

SBC PCB

5 J1

6 E6

7 E5

8 E4

1 SBC PCB

2 Fan

3 Power

6 LPT1

7 COM1

8 COM2

4 Hard Disk Drive 9 PC Keyboard

5 Floppy Disk Drive 10 LCD Display Screen

SDM PCB

1 SDM PCB

2 TP2 (DGND)

3 J9

4 TP1 PWR GND

SPM PCB

5 DS1 thru DS8 LEDs

6 J1 thru J8

7 J10

1 SPM PCB

2 R22 (WBC)

3 R27 (RBC)

4 R41 (PLT)

5 R51 (RER)

Touchpad PCB

6 E2 (AGND)

7 J1

8 J4

9 E1 (DGND)

10 E3 (AGND)

1 Touchpad PCB

2 J1

Vacuum/Pressure Regulator PCB (front)

3 J2

1 Vacuum /Pressure Regulator PCB 6 J1

2 P2

3 P1

4 TP3

5 TP1

Vacuum/Pressure Regulator PCB (rear)

7 DS1

8 R18

9 TP2

10 E1

1 Vacuum /Pressure Regulator PCB 2 R16

WBC Metering PCB

1 WBC Metering PCB

2 TP1

3 TP2

4 J1

5 TP3 (GND)

Removal & Replacement

Service Equipment Required

Every system subassembly and module can be removed and replaced using the tools listed below. Substitute equipment having the same characteristics as those listed can also be used. Whenever possible, choose non-magnetic tools to avoid damaging sensitive printed circuit board mounted components and magnetic media.

Required Tool Recommended Model

Cable Ties

Flashlight

Knife

Nut driver

Pliers

1/4"

1/2"

Description

Self-locking

Pen light

Utility

8-inch

Diagonal Cut

Pliers 8"

Screwdriver Phillips No. 2

Screwdriver Phillips No. 1

Screwdriver Phillips No. 2

Screwdriver Slotted 3/16 X 10

Screwdriver Slotted 3/16 X 8

Screwdriver Hex 7/64"

Spare tubing Silicone

Wrench

Long Nose

18-inch

Stubby

8-inch

Various lengths

Adjustable

Covers (CD18-A1)

Action

Remove

Upper Front

Cover

Removal

Steps

Grasp under the lower right side [2] and open the cover [1] toward the left.

Note

Cover is hinged on the left side and is kept closed with a magnetic latch.

2. Disconnect cable connector J1 [3] on the Status PCB [4].

3. Disconnect the ground wire [5] from the instrument chassis.

Use a slotted screwdriver to remove the

C clip [6] from the lower hinge.

Note

Be careful when removing the C clip.

The hinge contains a plastic washer [7] and a spring [8] which can be lost.

5. Remove the plastic washer and spring.

Lift the cover from the hinge pegs and remove it from the instrument.

Reference

Replacement

Action Steps

Install Upper Front Cover

Install in reverse order of disassembly.

Reference

Verification of Proper Cover

Installation

Action

Verification

Steps

1. Place instrument into READY mode.

2. From the MAIN Menu, press RUN followed by SPECIMEN TYPE, then

NORMAL BACKGRND.

Press the touch plate to run a cycle.

Reference

Note

During the cycle, make sure that cover does not interfere with instrument operation.

Once cycle is complete, verify acceptable background results. Refer to

Specifications & Checklist

Removal

Action

Open Upper

Front Cover

Check

Sample

Probe

Position

Steps

Open the upper front cover.

Caution

Be sure the sample probe is out of the way before attempting to remove the lower front cover to prevent damage to the probe.

• If the instrument is ON, from the MAIN

MENU press [SPECIAL PROTOCOLS] then [MORE] followed by [PROBE

HOME] to place the probe over the

RBC/PLT Mixing Chamber.

• If the instrument is OFF, make sure the

Sample Probe is raised all the way (if not, turn the lead screw to raise the probe) so

Reference

Remove

Lower Front

Cover that it clears the lower front panel.

1. Remove the thumbscrew [2] from the lower front cover (to the bezel).

Slide the lower front cover [1] to the left approximately 1/2-inch until it clears the bezel, then lift up and out.

Note

It may be necessary to lift the right side of the cover to clear the bezel indentation while sliding the cover to the left.

Verify that the upper left hand corner of the cover clears the clip. [3]

Replacement

Action Steps

Install Lower Front Cover

Install in reverse order of disassembly.

Reference

Verification of Proper Cover

Installation

Action

Verification

Steps

1. Place instrument into READY mode.

2. From the MAIN Menu, press RUN followed by SPECIMEN TYPE, then

NORMAL BACKGRND.

Press the touch plate to run a cycle.

Reference

Note

During the cycle, make sure that cover does not interfere with instrument operation.

Once cycle is complete, verify acceptable background results. Refer to

Specifications & Checklist

Action

Remove

Top Cover

Removal

Steps

1. Using a Phillips-head screwdriver, remove the two (2) mounting screws [2] on the rear of the instrument securing the top cover to the chassis.

Lift and remove the top cover [1] by sliding it to the rear.

Reference

Replacement

Action Steps

Install Top Cover

Install in reverse order of disassembly.

Reference

Action

Verification of

Proper Cover

Installation

Verification

Steps

1. Verify that cutouts on the front portion of the top cover [1] are placed under the locking nuts [2].

Be sure that rear of the cover is flush against the rear instrument panel.

Reference

Action

Open Upper

Front Cover

Remove

Lower Front

Cover

Remove

Right Bezel

Screw

Removal

Steps

Open the upper front cover to the left.

Remove Lower Front Cover

Lower Front Cover

(

RR-A1.02

Using a Phillips-head screwdriver, remove the left screw [2] on the right side cover [3].

Note

The Phillips screw secures the bezel to the instrument.

Reference

Remove

Lift the bezel up and away from the

Bezel instrument.

Note

Do not pull bezel too far away from instrument. Several cables must be removed before completely removing the bezel from the instrument.

Disconnect the following: o

Ground wire to chassis [7] o

Power [3] and data [2] (ribbon) cable connectors from the rear of the floppy disk drive [4] o

Ribbon connector the Touch Pad Interconnect

PCB [6].

Note

Mark all cable connectors and note pin 1

(red stripe on ribbon) on the floppy drive data cable.

securing the LCD Interconnect PCB access cover [1].

4. Disconnect the ribbon cable connected to

JP1A [9] on the LCD Interconnect

PCB [10].

Remove the bezel.

Action

Install

Bezel

Replacement

Steps

Install in reverse order of disassembly.

Note

During assembly, verify that all cables/connections are secured and are not pinched between the cover and the chassis.

Verification

Reference

Action

Verification of Membrane

Keypad Operation

Steps

ON instrument, and allow it to initialize.

2. During initialization, verify that the LED on the floppy disk drive turns on and that the LCD display screen is operational.

3. Once instrument is initialized, press RUN to prime system.

Reference

instrument response.

If system does not respond, check membrane keypad connection.

Action

Remove

Right Cover

Removal

Steps

1. Remove all cable connectors

(keyboard [3], COM1 [4], COM2 [5],

LPT1 [6]) from the right side of the instrument.

2. Using a Phillips-head screwdriver, remove the left and right screws [2] securing the right side cover to the instrument frame.

Slide the right side cover [1] to the rear of the instrument about 1/2-inch to clear the bezel locking nuts, and lift off.

Reference

Replacement

Action Steps

Install Right Cover

Install in reverse order of disassembly.

Reference

Verification

Verification of Proper Cover

Installation

Action Steps

1. Verify that cutouts on front and rear portion of cover are placed under locking nuts.

Be sure cover is secure against right side of instrument.

Action

Remove

Left Cover

Removal

Steps

1. Using a Phillips-head screwdriver, remove the left and right screws [2] securing the left side cover [1] to the instrument frame.

Slide the left cover to the rear of the instrument about 1/2-inch to clear the bezel locking nuts and lift off.

Reference

Replacement

Action Steps

Install Left Cover

Install in reverse order of disassembly.

Reference

Action

Verification

Steps Reference

Verification of Proper Cover

Installation

1. Verify that cutouts on front portion of cover are placed under locking nuts.

Be sure cover is secure against left side of instrument.

Flow Panel (CD18-B1)

Action

Prerequisite

Removal

Steps

Verify that the instrument is ON.

Open the upper front cover.

Open Upper

Front Cover

Remove Lower

Front Cover

Remove Lower Front Cover

(

RR-A1.02 Lower Front Cover

Remove RBC

Aperture Plate

1. From the MAIN MENU, press

[SPECIAL PROTOCOLS] then

[MORE] followed by [DRAIN

BATHS]:

2. Locate the von Behrens transducer and swivel the red Aperture Plate

Retaining Arm [2] outward.

Pull the Aperture Plate [1] straight out.

Reference

Remove WBC

Repeat the same procedure to

Aperture Plate remove the WBC Aperture Plate.

Note

Note the location of the cutout notch and WBC etched in the Aperture

Plate.

Action

Install

Aperture

Plate

Replacement

Steps

Install in reverse order of disassembly.

Note

The cutout notch [3] in the Aperture Plate must be facing down and toward the instrument when the plate is reinserted into the transducer. Also note the RP etched in the RBC Aperture Plate and WBC in the

WBC Aperture Plate.

Reference

Action

Verify Background

Counts

Verify Quality

Controls

Verification

Steps

Run a normal background count cycle and verify results are within acceptable limits. Refer to

Specifications & Checklist

Reference

Run three (3) levels of CELL-DYN controls and verify results are within acceptable assay limits.

Both the von Behrens RBC and WBC transducers are removed according to the following procedure:

Action

Prerequisite

Removal

Steps

1. If any fluid remains in the transducers, make sure the instrument is turned ON.

2. From the MAIN MENU, press

[SPECIAL PROTOCOLS] then

[MORE] followed by [DRAIN

BATHS].

Turn the instrument OFF after the transducers are drained.

Reference

Open Upper Front

Cover

Remove Lower Front

Cover

Remove Top Cover

Open the upper front cover.

Remove Lower Front Cover

(

RR-A1.02 Lower Front Cover

Remove Top Cover

(

RR-A1.03

Top Cover

Remove Bezel

Remove PAM PCB

Remove the bezel (

RR-A1.04

Bezel

Remove the Pre-Amplifier

Module (PAM) PCB (

RR-F1.01

PAM (Pre-Amplifier Module)

Prepare to Remove von Behrens WBC

Transducer

(connected to PAM) through the

Flow Panel.

Label and disconnect all tubing [1] from the von Behrens

WBC Transducer [3].

Remove von Behrens

WBC Transducer

1. Using a Phillips-head screwdriver, remove the three

(3) mounting screws [2] from the transducer holding plate.

2. Cut all cable ties securing the transducer cable to other cables/chassis.

Route transducer cable away from front panel and remove transducer.

Prepare to Remove von Behrens

RBC/PLT Transducer

(connecting to PAM) through the

Flow Panel.

Label and disconnect all tubing [3] from the von Behrens

RBC/PLT Transducer [1].

Remove von Behrens

RBC/PLT Transducer

1. Using a Phillips-head screwdriver, remove the three

(3) mounting screws [2] from the transducer holding plate.

Lift the transducer assembly [1] out of the instrument.

Replacement

Action Steps

Install von Behrens Transducer

Install in reverse order of disassembly.

Reference

Verification

Action Steps

Verify Gain Settings

Perform

VP-09 Signal Processor Module (SPM) Verification/Adjustment

Reference

Verify Background

Counts

Verify Quality

Controls

Run a normal background count cycle and verify results are within acceptable limits. Refer to

Specifications & Checklist

Run three (3) levels of CELL-DYN controls and verify results are within acceptable assay limits.

WBC Metering PCB Removal

Action

Prerequisite

Removal

Steps

1. Instrument must be in Initialized or

Ready mode.

2. From the MAIN menu, press [SPECIAL

PROTOCOLS] then [MORE] followed by [DRAIN BATHS]:

When the drain baths procedure is complete, turn OFF the instrument.

Reference

Open Upper

Front Cover

Remove Lower

Front Cover

Open the upper front cover.

Remove Lower Front Cover

(

RR-A1.02

Lower Front Cover

Remove Bezel

Remove the bezel (

RR-A1.04 Bezel

Remove

Plastic Shield

Using a Phillips-head screwdriver, remove the two (2) screws on the metering PCB transparent plastic shield.

Disconnect

Metering Tube

Tubing

Disconnect the tubing [4] at the top and bottom of the metering tube [3].

Remove WBC

Metering PCB

Caution

When removing tubing, take extreme care not to break the glass ends. The metering tube ends are fragile.

Use absorbent tissue to remove any fluid in the metering tube.

Caution

Take extreme care not to allow the liquid to touch the printed circuit boards; it can short them out when power is turned on. If the board does get wet, make sure it is dry before turning power back ON.

1. Using a 1/4 inch nut driver, remove the two (2) standoffs [7] on the board.

2. Remove the ground wire [5] on the bottom standoff.

3. Disconnect cable connector J1 [6] on the metering PCB.

4. Using a Phillips-head screwdriver, remove the two (2) mounting screws [2] securing the metering PCB to the Flow

Panel.

Lift the metering PCB [1] off of the Flow

Panel.

RBC/PLT Metering PCB Removal

Action

Prerequisite

Removal

Steps

1. Instrument must be in Initialized or

Ready mode.

2. From the MAIN menu, press [SPECIAL

PROTOCOLS] then [MORE] followed by [DRAIN BATHS]:

When the drain baths procedure is

Reference

complete, place the instrument in

Standby and turn OFF the power.

Open Upper

Front Cover

Remove Lower

Front Cover

Open the upper front cover.

Remove Lower Front Cover

(

RR-A1.02

Lower Front Cover

Remove Bezel

Remove the bezel (

RR-A1.04 Bezel

Remove

Plastic Shield

Using a Phillips-head screwdriver, remove the two (2) screws on the metering PCB transparent plastic shield.

Disconnect

Metering Tube

Tubing

Remove

RBC/PLT

Metering PCB

Disconnect the tubing [4] at the top and bottom of the metering tube [3].

Caution

When removing tubing, take extreme care not to break the glass ends. The metering tube ends are fragile.

Use absorbent tissue to remove any fluid in the metering tube.

Caution

1. Using a 1/4 inch nut driver, remove the two (2) standoffs [7] on the board.

2. Remove the ground wire [5] on the bottom standoff.

Disconnect the cable connector J1 [2] on the side of the metering PCB.

Note

The RBC Metering PCB [1] is installed

upside down. Push the metering tube all the way down to access the top screw securing the metering board to the flow panel.

4. Using a Phillips-head screwdriver, remove the two (2) mounting screws [6] securing the metering PCB to the Flow

Panel.

Lift the metering PCB off of the flow panel.

Replacement

Action Steps

Install Metering PCB

Install in reverse order of disassembly.

Note

Verify that tubing is not pinched when installing the plastic shield.

Reference

Action

Verify Count

Times

Verification

Steps

Verify appropriate count times (see

VP-04 Metering System Timing Adjustments -

RBC and WBC

: o

Verify RBC/PLT count times after installing RBC/PLT metering PCB.

Verify WBC count times after installing WBC metering PCB.

Reference

Action

Prerequisite

Removal

Steps

1. From the MAIN MENU, press

[SPECIAL PROTOCOLS] then

Reference

Open Upper

Front Cover

Remove Lower

Front Cover

Remove Bezel

[MORE] followed by [DRAIN BATHS]:

Turn instrument power OFF.

Open the upper front cover.

Remove Lower Front Cover

(

RR-A1.02 Lower Front Cover

Remove Top

Cover

Remove the bezel (

RR-A1.04 Bezel

Remove Top Cover

(

RR-A1.03 Top

Cover

Open Top Inner

Cover

1. Using a Phillips-head screwdriver, loosen the two (2) screws [1] securing the top inner cover [2].

Lift the top inner cover upward and to the right.

Note

This cover is hinged and stands in a folded back position without support.

Remove Top 1. Using a Phillips-head screwdriver,

Shield from

PAM PCB

Route HGB

Cable loosen the two (2) screws [4] securing the top shield [1] on the PAM PCB [2].

Remove the shield.

Disconnect the cable connector from

J2 [3] and route the HGB [7] cable through the hole [5] at the right side of the WBC metering PCB [6] and pull it through the hole.

Remove Tubing

Caution

The tubing is stiff. Use care when removing the tubing to avoid breaking the HGB Flow Cell.

Remove the black tubing from the

T-fitting [2] above and below the HGB

Flow Cell [1].

Remove

Hemoglobin

Flow Cell

Note

Use absorbent tissue to capture any excess liquid in the HGB Flow Cell.

1. Using a Phillips-head screwdriver, remove the two (2) screws [3] securing the HGB flow cell to the flow panel.

Route the HGB cable from behind the tubing installed at solenoid 2-6 and

remove the HGB Flow Cell module.

Replacement

Action Steps

Install Hemoglobin Flow Cell

Install in reverse order of disassembly.

Reference

Action

Verify HGB Offset, Gain,

Precision

Verification

Steps

HGB Self Test

and

Adjust HGB Gain

(see

Pre-Amp Alignments

Verify HGB precision.

Reference

Verify Background

Counts

Run a normal background count cycle and verify results are within acceptable limits. Refer to

Specifications & Checklist

Verify Quality Controls

Run three (3) levels of CELL-DYN controls and verify results are within acceptable assay limits.

Fluid Power Supply (CD18-C1)

Action

Prerequisite

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Reference

Remove Top

Cover

Remove Top Cover

(

RR-A1.03 Top

Cover

Open Top

Inner Cover

Remove

Tubing

Open Top Inner Cover

(

RR-B1.04

Hemoglobin Flow Cell

Note

Cut any cable ties on cables surrounding the fluid power supply assembly as needed.

1. Label and disconnect the tubing from the T-connector at the top of solenoid 5-6 [4].

2. Label and disconnect the tubing from solenoid 5-5 [3].

3. Label and disconnect the silicone tubing [1] from the top of the 0.5 psi accumulator located at the rear of the fluid power supply.

4. Label and disconnect the tubing from solenoid 5-1 [2].

5. Disconnect the tubing [5] from the chassis floor fitting [6] next to the left pump assembly.

Disconnect the tubing [7] connected to the reagent panel waste line (at the rear of the Reagent Inlet Panel).

Remove

Tubing

(continued) corresponding silicone tubing [1].

Follow the tubing to the back of the

Flow Panel, then label and disconnect the tubing at the in-line connector.

Remove the clamp [2] and plug [3] from the Vacuum Accumulator drain line [4], and pull the tubing through the rear of the Reagent Inlet Panel [5].

Disconnect

Connectors

1. Disconnect cable connector J6 [3] from the Pump Relay PCB [1].

2. Disconnect cable connector J1 [2] from the Pump Relay PCB [1].

3. Disconnect cable connector J1

(three-pin cable) from the internal waste sensor PCB located directly under the

Pump Relay PCB.

4. Disconnect cable connector J9 [3] from the Solenoid Driver board.

5. Disconnect cable connector J10 [4] from the Solenoid Driver board.

Disconnect cable connector J8 [2]

(bottom connector) from the Solenoid

Driver Module (SDM).

Remove Fluid

Power Supply

1. Using a Phillips-head screwdriver, remove the three (3) corner mounting screws and the one (1) central mounting screw securing the Fluid

Power Supply to the base of the chassis.

Lift the Fluid Power Supply directly up and out of the instrument.

Note

Verify that all cable connectors and tubing are disconnected.

Replacement

Action Steps

Install Fluid Power Supply

Install in reverse order of disassembly.

Reference

Action

Verify Vacuum, Pressure,

Count Times

Verification

Steps

VP-03 Vacuum and Pressure Adjustments

Verify all count times (see

VP-04 Metering System Timing

Reference

Adjustments - RBC and WBC

Syringe Assembly (CD18-E1)

Action

Prerequisite

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Open Upper

Front Cover

Remove Lower

Front Cover

Open the upper front cover.

Remove Lower Front Cover

(

RR-A1.02

Lower Front Cover

Remove Top

Cover

Remove Top Cover

(

RR-A1.03 Top

Cover

Open Top

Inner Cover

Remove

Connectors

Open Top Inner Cover

(

RR-B1.04

Hemoglobin Flow Cell

1. Mark and remove the cable connectors at the top of the cable distribution board [1]. There are four cable connectors that must be unplugged from this board: J20, J21, J22, and

J23.

Cut all cable ties securing cables to other cables (there are approximately

6-8 cable ties).

Reference

Remove Wash

Block Tubing

1. Using a 3/32-inch hex wrench, remove the clamp securing the aspiration tubing [3].

2. Using an adjustable wrench, loosen the screw securing the two (2) clamps [2] securing the wash block tubing.

3. Label and disconnect the two (2) tubes from the Wash Block (top is diluent and bottom is waste).

Route the clamps with attached tubing through the cutout [4] on the left side of the Sample Probe Driver assembly chassis [2].

Remove

Sample Probe

Tubing

Remove

Sample Probe

Motor Cable

Disconnect the tubing from the top of the Sample Probe.

Disconnect the cable connectors

J1 [2] [5] (ribbon) and J2 [3] [6] (power) from both the top [1] and bottom [4] chopper PCBs.

Remove

Sample Probe

Assembly

1. Using a Phillips-head screwdriver, remove the two (2) top and two (2) bottom mounting screws [1] securing the Sample Probe Driver Assembly to the chassis.

Lift the Sample Probe Driver Assembly out of the instrument.

Note

Before lifting the Sample Probe Driver

Assembly completely out of the instrument, check to make sure that all cables are disconnected. Disconnect any cables that you find still attached, making sure to mark them before unplugging.

Replacement

Action Steps

Install Sample Probe Assembly

Install in reverse order of disassembly.

Reference

Action

Verify Normal Operation

Verification

Steps

Run three (3) cycles and verify normal operation, e.g. binding, liquid

Reference

leaks, etc.

Perform

VP-12 Sample Probe Alignment Check

Verify Sample Probe

Alignment

Hazard

Action

Prerequisite

Remove Top

Cover

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Remove Top Cover

(

RR-A1.03 Top

Cover

Open Top Inner Cover

(

RR-B1.04

Hemoglobin Flow Cell

Open Top Inner

Cover

Remove Left Side

Cover

Remove the left side cover (see

RR-A1.06 Left Cover

Remove Fan

Note

The fan above the Diluent Syringe

Driver Assembly must be removed before the driver assembly can be removed.

Using a Phillips-head screwdriver, remove the two (2) top fan screws [1].

Note

Screws are secured by a nut. Verify that ALL nuts are removed prior to removing fan.

Using a Phillips-head screwdriver,

Reference

loosen the two (2) bottom fan bracket screws [2].

Note

If necessary, rotate the split pin installed on the directional valve drive linkage of the Diluent Syringe to allow the fan to be removed.

Move the fan to the rear.

Disconnect

Diluent Syringe

Driver Assembly

Disconnect the tubing [3] on both sides of the directional valve [2] at the top of the Diluent Syringe [1].

Note

Use absorbent tissue to capture any excess liquid.

2. Disconnect ribbon cable 9520444 from the back of the bottom chopper

PCB.

Disconnect ribbon cable 9520445 from the top chopper PCB.

Remove Diluent

Syringe Driver

Assembly

Note

Lyse Syringe Driver Assembly [1],

Diluent Syringe Driver Assembly [2], and Sample Syringe Driver

Assembly [3].

1. Using a 7/64-inch hex wrench, remove the two (2) hex screws [5] on the front of the base plate [4] located at the bottom of the diluent driver assembly [2].

2. Using a 7/64-inch hex wrench, loosen the hex screw [6] from the

rear base plate [4] of the Diluent

Syringe Driver Assembly [2].

Slide the Diluent Syringe Driver

Assembly [2] forward, tilt it back, and remove it through the right side of the chassis.

Replacement

Action Steps

Install Diluent Syringe Driver Assembly

Install in reverse order of disassembly.

Reference

Action

Verify Operation

Verify Quality

Verification

Steps

Verify

VP-10 Diluent and Sample Verification/Adjustment

Run three (3) levels of CELL-DYN controls and verify results are within

Reference

Controls acceptable assay limits.

RR-E1.04 Sample Syringe Driver Assembly

Action

Prerequisite

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Remove Top

Cover

Open Top Inner

Cover

Remove Top Cover

(

RR-A1.03 Top

Cover

Open Top Inner Cover

(

RR-B1.04

Hemoglobin Flow Cell

Remove Left Side

Cover

Remove Fan

Remove the left side cover (see

RR-A1.06 Left Cover

Remove the fan located above the

Diluent Syringe Driver Assembly

(see

Remove Fan

RR-E1.02

Diluent Syringe Driver Assembly

Disconnect

Tubing

Disconnect the tubing [2] on top of the Sample Syringe Driver

Assembly [1].

Reference

Disconnect

Connectors

1. Cut the cable ties securing the cable harness to the back of the syringe assembly.

Disconnect ribbon cable 9520441 from the chopper driver PCB.

Remove Sample

Syringe Driver

Assembly

Note

Lyse Syringe Driver Assembly [1],

Diluent Syringe Driver Assembly [2], and Sample Syringe Driver

Assembly [3].

1. Using a 7/64-inch hex wrench, remove the two (2) hex screws [5] on the front base plate [4].

2. Using a 7/64-inch hex wrench, loosen the hex screw [6] from the rear base plate [4] of the Sample

Syringe Driver Assembly [3].

Slide the Sample Syringe Driver

Assembly [3] forward, tilt it back, and remove it through the right side of the chassis.

Replacement

Action Steps

Install Sample Syringe Driver Assembly

Install in reverse order of disassembly.

Reference

Action

Verify Sample

Volume

Verify Quality

Controls

Verification

Steps

Verify sample volume (

VP-10 Diluent and Sample Verification/Adjustment

Run three (3) levels of CELL-DYN controls and verify results are within acceptable assay limits.

Reference

RR-E1.06 Lyse Syringe Driver Assembly

Action

Prerequisite

Removal

Steps

Place the instrument in Standby

Reference

and turn OFF the power.

Remove Top Cover

Remove Top Cover

(

RR-A1.03

Top Cover

Open Top Inner

Cover

Remove Left Side

Cover

Disconnect

Tubing/Cables

Open Top Inner Cover

(

RR-B1.04

Hemoglobin Flow Cell

Remove the left side cover (see

RR-A1.06 Left Cover

1. Disconnect the tubing [2] from the top of the Lyse Syringe [1].

Disconnect ribbon cable 9520446 from the chopper driver PCB.

Remove Lyse

Syringe Driver

Assembly

Note

Lyse Syringe Driver Assembly [1],

Diluent Syringe Driver

Assembly [2], and Sample Syringe

Driver Assembly [3].

1. Using a 7/64-inch hex wrench, remove the two (2) hex screws [5] on the front base plate [4].

2. Using a 7/64-inch hex wrench, loosen the hex screw [6] from the rear base plate [4] of the Lyse

Syringe Driver Assembly [1].

Slide the Lyse Syringe Driver

Assembly forward, tilt it back, and remove it through the right side of the chassis.

Action

Install Lyse Syringe Driver Assembly

Replacement

Steps

Install in reverse order of disassembly.

Reference

Action

Verify Quality

Controls

Verification

Steps

Run three (3) levels of CELL-DYN controls and verify results are within acceptable assay limits.

Electronics / Card Cage (CD18-F1)

RR-F1.01 PAM (Pre-Amplifier Module)

Action

Prerequisite

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Reference

Open Upper

Front Cover

Remove Lower

Front Cover

Remove Top

Cover

Remove Bezel

Open the upper front cover.

Remove Lower Front Cover

(

RR-A1.02 Lower Front Cover

Remove Top Cover

Cover

(

RR-A1.03 Top

Remove the bezel (

RR-A1.04 Bezel

Flip Bezel Latch

Bracket UP

1. Using a Phillips-head screwdriver, remove the lower screw [1] on the bezel latch bracket [2] attached to the chassis.

2. Loosen, but do not remove the top screw [3] on the latch.

Flip the latch up and to the right.

Remove Top

Shield from

PAM PCB

Remove PAM

Cover

1. Using a Phillips-head screwdriver, loosen the two (2) screws [4] securing the top shield [5] on the PAM PCB.

Remove the shield.

1. Using a Phillips-head screwdriver, remove the two (2) left retaining screws [4] [6] on the left side of the

PAM cover plate and loosen the two

(2) right retaining screws [7].

Slide the PAM cover [8] to the left and off the PAM PCB.

Disconnect

Cables

Remove PAM

Board

Mark and disconnect the five (5) cable connectors J1 [1], J2 [2], J3 [3],

J4 [4], and J5 [5] from the PAM PCB.

Note

The J3 connector is for WBC and the

J4 connector is for RBC/PLT.

1. Using a 1/4-inch nut driver, remove the four (4) metal standoffs [6], on the

corners of the PAM PCB.

Lift the PAM PCB off the Flow Panel.

Action

Install

PAM

Board

Replacement

Steps

Install in reverse order of disassembly.

Note

Be sure that the metal standoffs securing the PCB in place and the screws securing the metal cover over the PCB are securely fastened. Failure to properly secure the standoffs and cover screws may result in electrical noise being induced due to improper grounding.

Reference

Verification

Steps

Perform

VP-08 Pre-Amplifier Module (PAM) Adjustment

Perform

VP-09 Signal Processor Module (SPM)

Verification/Adjustment

Reference Action

Verify PAM Printed Circuit Board

Adjustments

Verify SPM Printed Circuit Board

Adjustments

RR-F1.02 MPM (Motor Processor Module) Board

Action

Prerequisite

Remove Top

Cover

Open Top

Inner Cover

Remove

Cables

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Remove Top Cover

Cover

(

RR-A1.03 Top

Open Top Inner Cover

(

RR-B1.04

Hemoglobin Flow Cell

Label and disconnect cable connectors

(leading to the MPM PCB): o

J1 [1] o

J2 [2] o

J3 [3] o

J4 [4] o

J5 [5] o

J6 [6] o

J7 [7] o

J10 [8]

Reference

Remove MPM

Board

At each corner of the MPM PCB, compress the plastic snap-in pins and pull the corner of the PCB free from the pin.

Note

Repeat this procedure for the snap-in pin at the center of the PCB.

Lift the MPM PCB off the top inner cover.

Replacement

Action Steps

Install MPM Board

Install in reverse order of disassembly.

Reference

Verification

Action Steps

Verify Motor Operation

Perform

VP-11 Stepper Motor Power Test and Verification

Reference

RR-F1.03 CDM (Cable Distribution Module) Board

Action

Prerequisite

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Remove Top

Cover

Remove Top Cover

(

RR-A1.03 Top

Cover

Open Top

Inner Cover

Disconnect

Cables

Open Top Inner Cover

(

RR-B1.04

Hemoglobin Flow Cell

Label and disconnect the following cable connectors (leading to the CDM

PCB): o

[15] o

[14] o

[13] o

[12] o

[11]

Reference

[10] o

[9] o

[8] o

[7] o

[16] o

[17] o

[18] o

[19] o

[20] o

[21] o

[22] o

[1] o

[2] o

[3] o

[4] o

[5] o

[23]

Remove CDM

Board

At each corner of the CDM PCB, compress the plastic snap-in pin and pull the corner of the PCB free from the pin.

Note

Repeat this procedure for the snap-in pin at the center of the PCB.

Lift the CDM PCB off the top inner cover.

Replacement

Action Steps

Install CDM Board

Install in reverse order of disassembly.

Reference

Action

Verification

Steps Reference

Verify CDM PCB

Voltage

Perform

VP-07 Cable Distribution Module Test Points

Verify Normal Operation

Run three (3) cycles and verify normal operation, e.g. binding, liquid leaks, etc.

RR-F1.04 Hard Disk Drive

Action

Prerequisite

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Open Upper Front

Cover

Remove Lower

Front Cover

Open the upper front cover.

Remove Lower Front Cover

(

RR-A1.02 Lower Front Cover

Remove Bezel

Remove the bezel (

Bezel

RR-A1.04

Remove Right

Side Cover

Remove the right side cover

(

RR-A1.05 Right Cover

Remove Front

Card Cage Shield

1. Using a Phillips-head screwdriver, remove the two (2) screws [1] securing the Front Card Cage

Shield [2] to the chassis.

Remove the shield from the instrument.

Reference

Prepare to

Remove Card

Cage from

Chassis

Remove Card

Cage

1. Disconnect the Backplane PCB

Power cable from the DC Regulator

PCB.

2. Disconnect the Disk Drive Power

Cable from the DC Regulator PCB.

3. Remove the two (2) screws securing the top section of the backplane

PCB shield and remove the shield.

Disconnect cable connectors J9 and

J10 on the Backplane PCB.

Using a Phillips-head screwdriver, remove the four (4) screws [1] securing the card cage [2] to the chassis.

Caution

Once the screws are removed, the card cage drops down. Excessive movement on the card cage can damage connectors on the

Backplane PCB.

Remove Hard

Disk Drive

Slide the bottom of the card cage forward and tilt it backward.

Note

Verify that all four (4) screws securing the hard disk drive to the card cage are accessible.

2. Using a Phillips-head screwdriver, remove the four (4) screws [1] securing the hard disk drive [2] to the card cage.

Note the alignment of the red stripe [5] on the ribbon cable in relationship to the hard disk drive connector.

Note

The same alignment must be maintained during replacement.

4. Disconnect the power [3] and data cable [4] connectors (rear of hard disk drive).

Remove the hard drive from the instrument.

Action

Replacement

Steps Reference

Install Hard Disk Drive

Install in reverse order of disassembly.

Verification

Action Steps

Verify Hard Disk Drive Setup

Perform

VP-14 Hard Disk Drive Setup and Verification

Reference

RR-F1.05 Floppy Disk Drive

Action

Prerequisite

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Open Upper

Front Cover

Remove Lower

Front Cover

Remove Bezel

(

Open the upper front cover.

Remove Lower Front Cover

RR-A1.02 Lower Front Cover

Remove Floppy

Disk Drive

Remove the bezel (

RR-A1.04 Bezel

1. Using a Phillips-head screwdriver, loosen the four (4) screws [1] securing the floppy drive mounting bracket [2] to the bezel.

2. Using a Phillips-head screwdriver, remove the four (4) screws [3] securing the floppy drive to the mounting bracket [4].

Remove the floppy disk drive from the instrument.

Reference

Replacement

Action Steps

Install Hard Disk Drive

Install in reverse order of disassembly.

Reference

Action

Verify Floppy Disk Drive

Operation

Verification

Steps

ON instrument, and allow it to initialize.

During initialization, verify that the LED on the floppy disk drive turns on and that the LCD display screen is operational.

Reference

RR-F1.06 Signal Processor Module (SPM)

Action

Prerequisite

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Open the upper front cover.

Open Upper

Front Cover

Remove Lower

Front Cover

Remove Bezel

Remove Front

Card Cage

Shield

Remove Lower Front Cover

(

RR-A1.02 Lower Front Cover

Remove the bezel (

RR-A1.04 Bezel

1. Using a Phillips-head screwdriver, remove the two (2) screws [1] securing the Front Card Cage

Shield [2] to the chassis.

Remove the shield from the instrument.

Reference

Remove SPM

PCB

Note

Card cage PCBs from left to right:

SPM, CCM, DLA and SBC.

1. Disconnect cable connector J1 [2] from the SPM PCB [1].

Open the top and bottom plastic mounting tabs.

Note

Pulls the board slightly out of the card cage.

Pull the board out of the instrument.

Action

Install SPM

PCB

Replacement

Steps

Install in reverse order of disassembly.

Note

When reinserting the SPM PCB into its slot at the rear of the card cage, press it firmly into place to ensure proper seating.

Reference

Action

Verify SPM PCB

Adjustments

Verify Background

Counts

Verification

Steps

Perform

VP-09 Signal Processor Module (SPM) Verification/Adjustment

Note

Make SPM adjustments in the following order:

RBC o

RER o

WBC o

PLT

Run a normal background count cycle and verify results are within acceptable limits. Refer to

Specifications & Checklist

Reference

Verify Quality Controls

Run three (3) levels of CELL-DYN controls and verify results are within acceptable assay limits.

RR-F1.07 Cell Count Module (CCM)

Action

Prerequisite

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Open the upper front cover.

Open Upper

Front Cover

Remove Lower

Front Cover

Remove Bezel

(

Remove Lower Front Cover

RR-A1.02 Lower Front Cover

Remove Front

Card Cage

Shield

Remove the bezel (

RR-A1.04 Bezel

1. Using a Phillips-head screwdriver, remove the two (2) screws [1] securing the Front Card Cage

Shield [2] to the chassis.

Remove the shield from the instrument.

Reference

Remove CCM

PCB

Note

Card cage PCBs from left to right:

SPM, CCM, DLA and SBC.

1. Disconnect cable connectors J1 through J3, and J5 from the CCM

PCB.

Open the top and bottom plastic mounting tabs [1].

Note

Pulls the board slightly out of the card cage.

Pull the board out of the instrument.

Action

Install CCM

PCB

Replacement

Steps

Install in reverse order of disassembly.

Note

When reinserting the CCM PCB into its slot at the rear of the card cage, press it firmly into place to ensure proper seating.

Reference

Action

Controls

Verify Background

Counts

Verify Quality

Verification

Steps

Run a normal background count cycle and verify results are within acceptable limits. Refer to

Specifications & Checklist

Reference

Run three (3) levels of CELL-DYN controls and verify results are within acceptable assay limits.

RR-F1.08 Data Link Adapter (DLA)

Removal

Action

Prerequisite

Open Upper

Front Cover

Remove Lower

Front Cover

Remove Bezel

Remove Front

Card Cage

Shield

Steps

Place the instrument in Standby and turn OFF the power.

Open the upper front cover.

Remove Lower Front Cover

(

RR-A1.02 Lower Front Cover

Remove the bezel (

RR-A1.04 Bezel

1. Using a Phillips-head screwdriver, remove the two (2) screws [1] securing the Front Card Cage

Shield [2] to the chassis.

Remove the shield from the instrument.

Reference

Remove DLA

PCB

Note

Card cage PCBs from left to right:

SPM, CCM, DLA and SBC.

1. Disconnect cable connectors J1 [2] and J2 [3] from the DLA PCB [1].

2. Using a Phillips-head screwdriver, remove the screw securing the DLA

PCB bracket to the top of the card cage.

Grasp the top and bottom of the DLA

PCB and pull the card from the card cage and away from the instrument.

Action

Install DCM

PCB

Replacement

Steps

Install in reverse order of disassembly.

Note

When reinserting the DLA PCB into its slot at the rear of the card cage, press it firmly into place to ensure proper seating.

Reference

Action

Verify Background

Counts

Verification

Steps

Run a normal background count cycle and verify results are within acceptable limits. Refer to

Specifications & Checklist

Reference

RR-F1.09 Single Board Computer (SBC)

Action

Prerequisite

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Open the upper front cover.

Open Upper

Front Cover

Remove Lower

Front Cover

Remove Bezel

(

Remove Lower Front Cover

RR-A1.02 Lower Front Cover

Remove Front

Card Cage

Shield

Remove the bezel (

RR-A1.04 Bezel

1. Using a Phillips-head screwdriver, remove the two (2) screws [1] securing the Front Card Cage

Shield [2] to the chassis.

Remove the shield from the

Reference

instrument.

Remove SBC

PCB

Note

Card cage PCBs from left to right:

SPM, CCM, DLA and SBC.

1. Label and disconnect all cable connectors from the SBC PCB [1].

2. Using a Phillips-head screwdriver, remove the screw securing the SBC

PCB bracket to the top of the card cage.

Grasp the top and bottom of the SBC

PCB and pull the card from the card cage and away from the instrument.

Action

Install SBC

PCB

Replacement

Steps

Install in reverse order of disassembly.

Note

When reinserting the SBC PCB into its slot at the rear of the card cage, press it firmly into place to ensure proper seating.

Reference

Action

Verification

Steps Reference

Verify Background

Counts

Run a normal background count cycle and verify results are within acceptable limits. Refer to

Specifications & Checklist

RR-F1.10 Card Cage Backplane PCB

Action

Prerequisite

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Open the upper front cover.

Open Upper

Front Cover

Remove Lower

Front Cover

Remove Bezel

(

Remove Lower Front Cover

RR-A1.02 Lower Front Cover

Remove Front

Card Cage

Shield

Remove the bezel (

RR-A1.04 Bezel

1. Using a Phillips-head screwdriver, remove the two (2) screws [1] securing the Front Card Cage

Shield [2] to the chassis.

Remove the shield from the instrument.

Reference

Remove SPM

PCB

Remove CCM

PCB

Remove SPM PCB

Remove CCM PCB

Remove DLA

PCB

Remove SBC

PCB

Remove

Backplane PCB

Shield

Remove DLA PCB

Remove SBC PCB

1. Using a Phillips-head screwdriver

(stubby), remove the top three (3) screws [2] securing the Backplane

PCB Shield [1] to the card cage [3].

2. Using a Phillips-head screwdriver

(stubby), remove the bottom three (3) screws [4] securing the Backplane

PCB Shield [2] to the card cage [3].

3. Disconnect cable connectors J1, J9, and J10 from the Backplane PCB.

Remove the shield from the instrument.

Remove

Backplane PCB

1. Verify that all screws and cable connections are removed from the

Backplane PCB.

Remove the Backplane PCB from the instrument.

Action

Replacement

Steps Reference

Install Backplane

PCB

Install in reverse order of disassembly.

Note

When reinserting the backplane PCB into its slot at the rear of the card cage, press it firmly into place to ensure proper seating.

Action

Verify Normal Operation

Verify Card Cage Backplane

PCB Voltages

Verify Background Counts

Verify Quality Controls

Verification

Steps

1. Place instrument in Ready mode.

Verify that instrument properly initializes and primes.

Perform

VP-06 Card Cage Backplane Test Points

Run a normal background count cycle and verify results are within acceptable limits. Refer to

Specifications & Checklist

Run three (3) levels of CELL-DYN Controls and verify results are within acceptable assay limits.

Reference

LCD/Keyboard (CD18-G1)

Action

Prerequisite

Removal

Steps

1. Place the instrument in Standby and turn OFF the power.

Unplug power cord from rear of instrument.

Open the upper front cover.

Open Upper

Front Cover

Remove Lower

Front Cover

Remove Lower Front Cover

Reference

Remove Bezel

Remove LCD

Display Screen

Assembly

(

RR-A1.02 Lower Front Cover

Remove the bezel (

RR-A1.04

Bezel

1. Place bezel cover face down on a flat surface.

2. Disconnect cable connector CN2 [3] from the backlight inverter PCB [4].

3. Release the backlight wires from the cable clip.

4. Using a Phillips-head screwdriver, remove the four (4) screws [5] securing the LCD Display Screen mounting plate to the bezel cover.

5. Using a Phillips-head screwdriver, remove the four (4) screws [1] securing the LCD Display Screen [6] to the mounting plate.

Disconnect the LVDS cable connector [7] at the rear of the LCD

Display Screen.

Note

When disconnecting the LVDS cable connector, pull the connector, NOT the wires.

Action

Install the LCD Display

Screen Assembly

Replacement

Steps

Install in reverse order of disassembly.

Note

During replacement, verify that the LCD Display Screen assembly is centered in the bezel cover, before securing the assembly to the cover.

Reference

Action

Verify LCD

Display Screen

Installation

Verification

Steps

1. Turn the instrument Power ON and allow the instrument to initialize.

2. Verify that the LCD Display Screen is turned on and centered in the bezel.

Verify that the LVDS cable (between the LCD display [3] and the LCD

Interconnect PCB [1]) is routed correctly [1-3]

Note

Failure to properly route and secure the LVDS cable may result in damage to the wires and connector.

Power Supply (CD18-H1)

Action

Prerequisite

Removal

Steps

Place the instrument in Standby and turn OFF the power.

Note

Never touch a PCB when the power is ON.

Unplug power cord from rear of instrument.

Open the upper front cover.

Open Upper Front

Cover

Remove Lower

Remove Lower Front Cover

Reference

Front Cover

Remove Top

Cover

Remove Bezel

Remove Right

Side Cover

Remove Cable

Connectors from

Power Switch

Remove Top Cover

(

RR-A1.03 Top

Cover

(

RR-A1.02 Lower Front Cover

Remove the bezel (

Bezel

RR-A1.04

Remove the right side cover

(

RR-A1.05 Right Cover

Label and disconnect the four (4) cable connectors at the rear of the power switch [1].

Note

The power switch contains two (2) black and two (2) white cable connectors.

Remove Cable

Connectors from

DC Regulator PCB

Label and disconnect cable connectors J3 through J9, and J11 from the DC Regulator PCB [2].

Remove Cable

Connectors from

AC Regulator PCB

Note

Cut cable ties from cable bundles containing cable connector J7.

1. Label and disconnect the cable connectors J5, J7 and J9 from the

AC Regulator PCB [1].

2. Label and disconnect the chain cable connector J6 from the AC

Regulator PCB and J2 from the DC

Regulator PCB.

Release the chain cable connector

Remove Power

Supply Assembly from the cable clamp attached to the switching power supply.

1. Using a Phillips-head screwdriver, remove the four (4) screws [1] from the rear of the instrument securing the power supply assembly to the instrument chassis.

2. Carefully lift the power supply up, then partially out of the instrument.

Verify that no cable connectors are connected to the power supply assembly.

Caution

The power switch is vulnerable to damage during this process.

Remove the power supply assembly.

Replacement

Action Steps

Install Power Supply Assembly

Install in reverse order of disassembly.

Reference

Verification

Action Steps

Verify System Voltages

Perform

VP-06 Card Cage Backplane Test Points

Reference

Verification Procedures

VP-01 Preparation for Alignment and Verification

VP-01 Preparation for Alignment and Verification

Purpose

Preparation for Alignments/Verifications.

Module CELL-DYN 1800

Materials Required

See

Test Equipment and Supplies Required

. Time 01:00 hr

Test Equipment and Supplies Required

ITEM DESCRIPTION

1 Digital Volt Meter

3 5-inch Jumper Leads

4 Stopwatch

5 Vacuum Gauge 0-30 Inches

6 Pressure Gauge 0-5 lb.

7 Pressure Gauge 0-10 lbs.

8 Polystyrene Microspheres, 5.0 or 5.01 µm

9 Polystyrene Microspheres, 3.31 µm

10 Hemostats

11 500 mL Flask or Beaker

12 Silicone Tubing

QTY

13 Standard Tool Kit 1

14 Fresh Blood Samples With Reference Values AR

15 Assayed Controls for CELL-DYN 1800

16 20k Ohm 1% Resistor

15k Ohm 1% Resistor

10 mL Graduated Cylinder

19 40 µL Micropipettes

20 100 µL Micropipettes

21 50 mL Counting Cups

25 mL Graduated Cylinder

Oscilloscope (Optional)

12-inch Ruler With 1/16 Increments

AR = As Required

Preparation for Alignment/Verification

Perform the following procedure to prepare the CELL-DYN 1800 System for alignment/verification:

Action

Check Reagents

Steps

Verify all reagents are correct and are 1/3 full.

Remove Upper Front Cover

(

RR-A1.01 Upper Front Cover

Remove Upper

Front Cover

Remove Lower

Front Cover

Remove Left

Cover

Remove Right

Cover

Remove Top

Remove Lower Front Cover

(

RR-A1.02 Lower Front Cover

Remove Left Cover

(

RR-A1.06 Left Cover

Remove Right Cover

(

RR-A1.05 Right Cover

Remove Top Cover

(

RR-A1.03 Top Cover

Reference

Cover

Clean Aperture

Plates

Clean HGB Flow

Cell

Re-initialize

Instrument

Check Tubing,

Look For Leaks

Install Covers

Run Background

Count

Remove and clean both RBC/PLT and WBC aperture plates following the procedure in Section 9: Service and Maintenance, Subsection:

Cleaning/Replacing Aperture Plates of the CELL-DYN 1800 Operator's Manual.

Clean the HGB Flow Cell following the procedure in Section 9, Subsection:

Cleaning the HGB Flow Cell of the CELL-DYN 1800 Operator's Manual.

Re-initialize the instrument by turning the system OFF then ON again.

When Initialization is complete, press [PRIME/RUN] to prime the instrument.

Observe the flow system for leaks, tubing placement, pinched tubing, etc.

Reinstall the covers on the instrument.

Run a Background count. Verify all background values are within the following specifications:

WBC 0.5 K/µL

RBC 0.05 M/µL

HGB 0.1 g/dL

PLT 10.0 K/µL

1. In the MAIN MENU, press [CALIBRATION].

Record all Calibration Factors for Open and Pre-Dilute modes.

Record

Calibration

Factors

Record Dilution

Factors

Enter Service

Personnel ID

Type 94043 to display the DILUTION FACTORS screen. Record all Dilution

Factors.

In the MAIN MENU, type 999 for Operator ID and press Enter on the PC keyboard or membrane keypad. This is to identify all runs performed by service personnel.

Order of Alignment/Verification

The following procedures are presented to ensure proper alignment of the CELL-DYN 1800 System.

Sequence Procedure

VP-03 Vacuum and Pressure Adjustments

VP-04 Metering System Timing Adjustments - RBC and WBC

VP-06 Card Cage Backplane Test Points

VP-07 Cable Distribution Module Test Points

VP-08 Pre-Amplifier Module (PAM) Adjustment

VP-09 Signal Processor Module (SPM) Verification/Adjustment

VP-10 Diluent and Sample Verification/Adjustment

VP-11 Stepper Motor Power Test and Verification

VP-12 Sample Probe Alignment Check

When performing adjustments:

• At the completion of each Vacuum and Pressure Adjustment procedure, verify the WBC and RBC count times and normal system operation

• Prior to starting any electronic alignment, verify the power supply voltages in

VP-06 Card Cage Backplane Test

Points

and

VP-07 Cable Distribution Module Test Points

• Whenever an alignment adjustment is performed on the Pre-Amplifier Module (PAM), verify all parameters on the

Signal Processor Module (SPM).

VP-02 Decontamination

Purpose

To decontaminate all surfaces before servicing. Module CELL-DYN 1800

Materials Required

Sodium hypochorite solution

Time

00:15 min

Action

Decontamination

Ratio

Steps

The OSHA Bloodborne Pathogen Rule (29 CFR Part 1910.1030) requires the decontamination of laboratory equipment prior to servicing or shipment: o

Decontaminate the instrument by performing the Auto-Clean cycle. This cycle flushes all of the fluid pathways with reagents to purge any waste from the fluid pathways. The Open Mode Sample Probe is automatically rinsed

Reference

after every cycle. The surfaces of the instrument should be wiped with a nonabrasive detergent solution to remove any soiling, then wiped with a tuberculocidal disinfectant, such as a 0.5% sodium hypochlorite solution.

To calculate the percent (%) sodium hypochlorite concentration desired, use the following formula: a. A = Percent (%) of sodium hypochlorite solution desired b. B = Percent (%) of sodium hypochlorite stock solution (as purchased) c. X = Parts of water to be mixed with one part of the sodium hypochlorite stock solution: d. Example: If you need a 0.5% solution of sodium hypochlorite for a cleaning procedure, and the label on the bottle of bleach states that it is 5.25% sodium hypochlorite, then: e.

Add 9.5 parts deionized water to 1 part bleach to obtain a 0.5% sodium hypochlorite solution, or 9.5 mL of deionized water to 0.5 mL of bleach

(5.25% sodium hypochlorite) to obtain 10 mL of a 0.5% solution of sodium hypochlorite.

If the instrument is to be shipped, it must be decontaminated prior to shipment. This is accomplished by pressing the [CLEAN FOR SHIPPING] key in the SPECIAL

PROTOCOLS menu. Instructions for this procedure are given in the

CELL-DYN 1800 Operator's Manual, (Section 9 Preparing the Instrument for

Extended Periods of Non-Use or Shipping).

VP-03 Vacuum and Pressure Adjustments

Purpose

Materials Required

Align and adjust pressure and vacuum. Module CELL-DYN 1800

Digital Volt Meter (DVM)

0-5 psi Pressure Gauge

0-30 psi Pressure Gauge

0-30 HG Vacuum Gauge

Phillips Screwdriver

Thin Flat Blade Screwdriver

Time

00:30 min

This procedure consist of four parts (perform in sequence):

Regulator Alignment

Pressure Adjustment (0.5 psi)

Pressure Verification (High)

Vacuum Adjustment (8 inch)

The CELL-DYN 1800 System utilizes one vacuum and two pressure levels to accomplish the following tasks: moving sample, reagents, and waste, bubble mixing of sample, and back flushing RBC and WBC apertures. The vacuum and bubble mix pressures are adjustable by a solid-state regulator. The backflush pressure is not critical and the pump is under direct computer control.

The solid state regulator has two input ports: P1 for pressure and P2 for vacuum. It also has jumper terminals which accommodate all desired vacuum and pressure ranges. The jumper positions are:

Jumpers Pressure (psi) Vacuum (inches Hg)

1 A-B 0.0 - 3.0

2 C-D 2.5 - 5.1

3 E-F 4.3 - 6.6

4 G-H 6.0 - 8.0

0.0 - 6.0

5.0 - 10.2

8.6 - 13.2

12.0 - 16.0

Regulator Alignment

Action

Access Pump

Relay Board

Steps

Note

This offset adjustment must be performed with no pressure or vacuum applied to the regulator.

Reference

Disconnect the appropriate pumps on the Pump Relay Board and bleed off

Measure Voltage

Reposition E1 pressure from the Accumulators.

J4 for low pressure o

J2 for vacuum on new-style pumps

Remove the pressure or vacuum line from the top of the regulator.

On the Pump Relay Board the voltage should be 5.0 ± 0.15 volts at J7 pin 7 for vacuum, and J8 pin 5 for pressure.

Note

You must remove the board mounting screws and free the rest of the board to make the remaining adjustments. Disconnect J6 or J7 while relocating the appropriate printed circuit board to avoid shorting out components.

2. Note the current position of jumper E1 and set E1 to the C-D position.

Connect the DVM (Digitial Volt Meter) negative lead to TP3 (GND) on regulator board. Connect the positive lead to TP2 (REF). The voltage should be

1.00V ± 0.14 volts.

If voltage is not correct, re-check

Step 1

If voltage is still not correct, replace the regulator.

Connect the DVM negative lead to TP1 and the positive lead to TP2, and read the voltage. Adjust R18 for a voltage of 0.000 ± 0.005.

Note

If voltage is negative, turn R18 (offset) clockwise; if the voltage is positive, turn R18 counterclockwise.

Move jumper E1 back to the proper operating position.

Reconnect

Pressure / Vacuum

Line

Reconnect the pressure or vacuum line to the top of the regulator and reconnect the cable(s) on the pump relay module.

Pressure Adjustment (0.5 psi)

Follow the steps below for pressure adjustment:

Action

Remove Top

Cover

Steps

Remove Top Cover

(

RR-A1.03 Top Cover

Reference

Open Top Inner

Cover

Connect

Pressure Gauge

Open Top Inner Cover

(

RR-B1.04 Hemoglobin Flow Cell

1. Locate the small silicone tubing connected to the in-line fitting at top of the 0.5 psi

Pressure Accumulator (located closest to the rear of the instrument).

Connect a 0-5 psi gauge in-line with the silicone tubing and fitting (the Pressure

Accumulator is located on the part of the Fluid Power Supply which is closest to the rear of the instrument).

Adjust

Regulator

Adjust R16 on the Regulator board for 0.55 psi ± 0.02.

Note

Clockwise adjustment increases pressure and counterclockwise adjustment decreases pressure.

Remove the gauge and reconnect the silicone tubing to in-line connector.

Pressure Verification (High)

Follow the steps below for pressure verification:

Action

Locate In-line

Connector

Steps

Locate solenoid valve 1-6 and trace the tubing back through the flow panel to the in-line connector.

Reference

Connect Pressure

Gauge

Verify Correct

Pressure

1. Connect a 0-30 psi gauge in-line.

In the RUN screen, press [CLEAR ORIFICE] while observing the gauge.

When the pump activates, verify a pressure of no less than 4 psi.

If the pressure is less than 4 psi, check for leaks. Replace the Pressure

Pump if necessary. (Refer to

RR-C1.01 Fluid Power Supply

Vacuum Adjustment (8 inch)

Follow the steps below for solid state regulator adjustment:

Action Steps

Locate Vacuum

Regulator

Note

This is a coarse adjustment only. The fine adjustment is predicated upon metering system count times. Make this coarse adjustment only if there is reason to believe that the vacuum is grossly misadjusted.

Reference

Locate the solid state vacuum regulator on the rear side of the Fluid Power Supply

(closest to the flow panel).

Adjust R16

Reconnect

Tubing Line

1. Remove the TYGON tubing from the top of the Vacuum Accumulator (located on the back of the Fluid Power Supply closest to the flow panel) and connect a 0-30" Hg gauge in-line between the tubing and the Accumulator.

Adjust R16 (accessible only from the front of the Fluid Power Supply) for

8.25" Hg ± 0.25".

Note

Clockwise adjustment increases vacuum and counterclockwise adjustment decreases vacuum.

Once R16 is adjusted, remove the vacuum gauge and reconnect the tubing line to the regulator.

VP-04 Metering System Timing Adjustments - RBC and WBC

Purpose

Adjust Metering System to regulate count times. Module CELL-DYN 1800

Materials Required

Phillips Screwdriver

Time

00:15 min

This procedure consist of two parts:

Links

RBC Metering System Timing Adjustment

WBC Metering System Timing Adjustment

The instrument uses the Volumetric Metering process to regulate the count cycle and to ensure that a precise volume of sample is analyzed for the measurement. This table lists the results of a fault report displayed on the screen if a flow error or clog occurs during a run cycle.

Metering Timing Fault Report

Detector Masked

(in seconds)

Flow Error Time

(in seconds)

Valid Meniscus Time

(in seconds)

0 - 0.5

0.5 - 1.0

1.0 - 3.0

3.0+

WBC Upper

WBC Lower

0 - 3.5

RBC/PLT Upper

0 - 3.0

RBC/PLT Lower

0 - 5.5

3.5 - 4.0

3.0 - 4.0

5.5 - 6.0

4.0 - 6.5

4.0 - 6.0

6.0 - 7.5

Clog Time

(in seconds)

6.5+ or greater than the moving average

6.0+

7.5+ or greater than the moving average

This figure illustrates the timing relationships for WBC and RBC measurements.

CELL-DYN 1800 Metering Timing Chart (in Seconds)

For each transducer there are two distinct counting periods, T1 and T2.

RBC Metering Tube

and

WBC Metering Tube

illustrate the counting periods for the RBC and WBC metering tubes, respectively.

RBC Metering Tube

WBC Metering Tube

All count times are based on the vacuum level of the 8-inch vacuum reservoir and tubing length. These count times are critical in that all clog and flow system alarms are generated via these count times. The vacuum adjustment (

Vacuum

Adjustment (8 inch)

) sets the coarse vacuum requirement, but further adjustments are necessary to ensure proper timing for the flow system alerts.

RBC Metering System Timing Adjustment

Follow the steps below for adjusting the timing of the RBC metering system:

Action

Verify

Count

Time

Steps

1. Ensure that the RBC aperture plate has been removed, cleaned, and reinstalled following the procedure in Section 9: Service and Maintenance, Subsection: Cleaning/Replacing

Aperture Plates of the CELL-DYN 1800 Operator's Manual.

Run a background count and verify that the RBC displayed count time (T2) is 6.7 seconds

± 0.2 seconds.

If the specification is met, go to

Step 4

If the specification is not met, go to

Step 3

3. Readjust the vacuum to correct the count time if the time is out of specification (refer to

Adjust R16

Step 2

of the Vacuum Adjustment (8-inch) procedure previously discussed).

4. From the MAIN Menu, press [DIAGNOSTICS] followed by [RAW DATA].

Verify the time displayed for RBC UPTIME is 4.8 to 5.2 seconds. (This time, displayed in milliseconds, represents T1, which is the time when the RBC valve 1-2 opens until the meniscus reaches the upper detector.)

Reference

If the specification is met, the procedure is complete.

If the specification is not met, go to

Step 6

Adjust the metering tube.

Raise the metering tube to increase the upper time.

Lower the metering tube to decrease the upper time.

Repeat

Step 2

through

Step 5

until the RBC Up Time/Count Time is within specification.

WBC Metering System Timing Adjustment

Follow the steps below for adjusting the timing of the WBC metering system:

Action

Prerequisite

Note

Steps

The RBC count times (T1 & T2) must be within specification before performing this procedure.

Verify Count

Time

1. Remove and clean the WBC aperture plate as described in Section 9: Service and

Maintenance, Subsection: Cleaning/Replacing Aperture Plates of the

CELL-DYN 1800 Operator's Manual.

Run a background count and verify the count time displayed to the right of the WBC histogram is 5.0 ± 0.5 seconds.

Note

There is no count time adjustment procedure.

3. From the MAIN Menu, press [DIAGNOSTICS] followed by [RAW DATA].

Verify that the time displayed for WBC UPTIME is 1.8 to 2.2 seconds. (This time represents T1 which is the time when the WBC valve 4-3 opens until the meniscus reaches the upper detector. See

WBC Metering Tube

.) o

If the WBC UPTIME specification is met, the procedure is complete, o

If the WBC UPTIME specification is not met, go to

Step 5

Adjust the metering tube.

Raise the metering tube to increase the upper time.

Lower the metering tube to decrease the upper time.

Repeat

Step 1

through

Step 4

until WBC UPTIME/COUNT TIME is within specification.

Reference

VP-05 CMOS Setup Verification/Adjustment

Purpose

Provide instructions to verify and/or modify CMOS settings. Module CELL-DYN 1800

Materials Required

None

Time

00:10 min

Action

Preparation

Power OFF the system.

Steps Reference

Access CMOS Setup

Utility Screen

Power ON the analyzer and press the Delete key, when prompted, to display the CMOS Setup Utility Screen.

Verify/Modify

Standard CMOS

Setup

1. Use the up/down arrow keys to select the STANDARD CMOS SETUP.

Enter key.

Refer to the figure (H2_6006) and make any necessary adjustments to the

Standard CMOS Setup screen.

Note

Press the up/down arrow keys to select a setting and then press PgUp/PgDn keys to modify.

Press ESC to exit the screen.

Verify/Modify

Advanced CMOS

Setup

1. Use the up/down arrow keys to select the ADVANCED CMOS SETUP.

Enter key.

Refer to the figure and make any necessary adjustments to the Advanced

CMOS Setup screen.

Note

Press the up/down arrow keys to select a setting and then press PgUp/PgDn keys to modify.

Press ESC to exit the screen.

Verify/Modify

Advanced Chipset

Setup

1. Use the up/down arrow keys to select the ADVANCED CHIPSET SETUP.

Enter key.

Refer to the figure and make any necessary adjustments to the Advanced

Chipset Setup screen.

Note

Press the up/down arrow keys to select a setting and then press PgUp/PgDn keys to modify.

Press ESC to exit the screen.

Verify/Modify Power

Management Setup

Note

The SAMSUNG LCD assembly is Type 8, while the TOSHIBA LCD assembly is Type 7.

1. Use the up/down arrow keys to select the POWER MANAGEMENT SETUP.

Enter key.

Refer to the figure and make any necessary adjustments to the Power

Management Setup screen.

Note

Press the up/down arrow keys to select a setting and then press PgUp/PgDn keys to modify.

Press ESC to exit the screen.

Verify/Modify

Peripheral Setup

1. Use the up/down arrow keys to select the PERIPHERAL SETUP.

Enter key.

Refer to the figure and make any necessary adjustments to the Peripheral

Setup screen.

Note

Press the up/down arrow keys to select a setting and then press PgUp/PgDn keys to modify.

Press ESC to exit the screen.

Save CMOS Settings and Exit CMOS Setup

Verification

Press F10.

The message Save current settings and exit (Y/N)? displays.

Type Y and press Enter.

Note

Saves the CMOS settings, exits the CMOS Setup Utility and restarts the computer.

Verify that the system boots up properly to the application software.

VP-06 Card Cage Backplane Test Points

Purpose

Identify test points on Backplane PCB. Module CELL-DYN 1800

Materials Required

Digital Volt Meter (DVM)

Time

00:10 min

The following table lists the test points located on the backplane board and the figure illustrates the test points on the backplane board.

Test Function Range

TP1 +5V +5.00V ± 0.20V

TP2 +12V Analog +12V ± 0.50V

Ripple

TP3 -12V Analog -12V ± 0.50V

TP4 +12V Digital +12V ± 0.50V

TP5 -12V Digital -12V ± 0.50V

Card Cage Backplane (Rear Side)

Card Cage Backplane (Card Side)

1 LD4 +12VDC (DIG)

2 LD5 -12VDC (DIG)

LD2 -12VDC (ANALOG)

LD1 +5VDC

3 LD3 +12VDC (ANALOG)

Note

LEDs indicate voltages supplied to the board.

VP-07 Cable Distribution Module Test Points

Purpose

Identify test points on CDM.

Module CELL-DYN 1800

Materials Required

Digital Volt Meter (DVM)

Time

00:10 min

The following table lists the test points located on the Cable Distribution Module (CDM).

Location

Pin

(J5 and J8)

Function Voltage Ripple

1 Brown Ground < 0.05V

N/A

2 Blank N/A N/A N/A

3 Orange Ground < 0.05V

N/A

4 Yellow +14V ±0.5V

N/A

5 Green +28V ±1.00V

N/A

6 Blank N/A N/A N/A

Note

On the CDM, +28V is used to initially energize solenoids and +14V is used to keep them closed.

VP-08 Pre-Amplifier Module (PAM) Adjustment

Purpose

Adjust HGB self test and gain.

Adjust RBC/PLT and WBC aperture currents.

Module CELL-DYN 1800

Materials Required

Digital Volt Meter (DVM)

Flat Blade Screwdriver

Phillips Screwdriver

5-inch Jumper Leads

20K Ohm 1% Resistor

15K Ohm 1% Resistor

Time

00:40 min

The Pre-Amplifier Module (PAM) is located on the upper right corner of the Flow Panel. Hemoglobin circuitry, and the

RBC/PLT and WBC aperture currents require verification and/or adjustment on this module. The HGB flow cell should be

cleaned before performing hemoglobin alignments (refer to Section 9, Subsection: Cleaning the HGB Flow Cell in the

CELL-DYN 1800 Operator's Manual).

Pre-Amplifier Module (PAM) Test Points

The following table lists the test points located on the PAM.

Location

TP5

TP4

TP7

Function

Analog Ground

+100V

Voltage

N/A

+100V ± 0.5V

HGB Self Test/Gain Adjust +1.84V ± 0.02V (Self Test)

+5.2V ± 0.2V (Gain Adjust)

Test points for the PAM are shown in the figure below.

Pre-Amp Module (PAM) Test Points

Follow the steps below to align the PAM:

Action Steps Reference

Open Upper Front Cover

Remove Lower Front Cover

Remove Top Cover

Remove Bezel

Remove Pre-amp Cover

HGB Self Test

Adjust HGB Gain

Verify +100 Volts

Adjust RBC/PLT Aperture

Current

Open the upper front cover.

Remove Lower Front Cover

(

RR-A1.02 Lower Front Cover

Remove Top Cover

(

RR-A1.03 Top Cover

Remove the bezel (

RR-A1.04 Bezel

Remove the pre-amp cover (

RR-F1.01 PAM (Pre-Amplifier Module)

JMP3 (lower right corner).

2. Press the touch plate to cycle the instrument and fill the HGB flow cell with fresh reagent.

3. Connect the positive lead of the DVM to TP7.

4. Connect the ground of the DVM to TP5.

R14 to 1.84 volts ± 0.02 volts.

6. Verify the voltage at TP7.

Connect jumper JMP3 and verify voltage at TP7 returns to 5 volts.

Note

If voltage remains unchanged, the jumper may be in the wrong position.

1. Press the touch plate to cycle the instrument and fill the HGB flow cell with fresh reagent.

2. Verify the voltage at TP7.

R4 (HGB Gain Adjust) for 5.2 volts ± 0.2 volts.

4. Press the touch plate to cycle the instrument and fill the HGB flow cell with fresh reagent.

Verify the voltage at TP7.

Note

If voltage does not meet specification, repeat Step 2 and Step 3 .

1. Connect the positive lead of the DVM to TP4, and the negative lead to

TP5 (Gnd).

Verify 100 volts ±0.5 volts.

1. Disconnect the RBC signal cable from J4.

2. Connect a 20K Ohm 1/4 watt resistor across pins 4 and 5 of J4.

3. Connect the DVM across the resistor.

4. Run a background count.

Adjust WBC Aperture

Current

Install Covers

Remove the resistor and reconnect cable to J4.

1. Disconnect the WBC signal cable from J3.

2. Connect a 15K Ohm 1/4 watt resistor across pins 4 and 5 of J3.

3. Connect the DVM across resistor.

4. Run a background count.

R66 (WBC aperture current adjust) for 12.0 volts ± 0.05 volts.

Remove the resistor and reconnect cable to J3.

Reinstall the top cover, upper and lower front covers, bezel and pre-amp cover.

The following table contains the specifications for the Pre-Amp Module alignment.

Pre-Amp Alignments

HGB SELF TEST

TP7 R14 +1.84V ± 0.02 V

HGB GAIN

TP7 R4 5.2 V. ± 0.2 V

RBC/PLT APERTURE CURRENT

20K Ohm Resistor R72 11.0 V. ± 0.05 V

15K Ohm Resistor R66 12.0 V. ± 0.05 V

WBC APERTURE CURRENT

VP-09 Signal Processor Module (SPM) Verification/Adjustment

Purpose

Materials Required

Adjust WBC, RBC, PLT Gain. and RBC Edit Ratio (RER) percentage (%).

Module

CELL-DYN 1800

Digital Volt Meter (DVM)

Thin Flat Blade Screwdriver

Polystyrene Microspheres, 5.0 or 5.01 µm

Polystyrene Microspheres, 3.31 µm

Clean 50 mL Container

Time

00:50 min

Note

The Signal Processor Module (SPM) is located in the main electronics card cage. Alignment of the WBC, RBC, and

PLT gains are critical adjustments that must be verified and/or adjusted before instrument accuracy can be established. It is important to maintain the adjustment order for this verification procedure. The adjustment order is:

Sequence

Activity

RBC Gain

RER Adjustment

WBC Gain

PLT Gain

Latex particles are used to perform these adjustments. The particles must be mixed vigorously before diluting to obtain accurate results.

The Gain and RBC Cell Editing adjustments are performed in the Gain Adjust Mode, which allows multiple counts to be run on the same sample. When Gain Adjust Mode is entered, whatever is in the Pre-Mix Cup is transferred to the WBC Cup and

5 mL of diluent is left undisturbed in the RBC Cup.

When performing RBC or PLT adjustments, 10 mL of diluent must be placed in Pre-Mix Cup before entering Gain Adjust

Mode to prevent air from being pulled into the von Behrens WBC Transducer during a count cycle.

Test points for the SPM are shown in the figure below:

SPM Test Points

RBC Gain

Action

Prepare Latex

Dilution

Set RBC Gain

Steps

1. Pour 10 mL of diluent into the Pre-Mix Cup and add 1 drop of well-mixed 5.0 latex particle solution to the 5 mL of diluent already in the RBC Mixing Chamber.

2. From the RUN screen, press [SPECIMEN TYPE].

Press Shift and # (simultaneously) to activate the Gain Adjust Mode.

Reference

Note

The message <PREPARING FOR GAIN ADJUST.> displays and WBC dilution is transferred to the WBC Mixing Chamber. When the preparation cycle is complete, the RUN screen displays.

Press the Touch Plate to run a cycle.

Note

Always return to the RUN screen to run a cycle.

Note

When the procedure is completed, or if there is a need to exit the Gain Adjust Mode while performing this procedure, such as constant clogs or improper dilution ratio, press [SPECIMEN TYPE] followed by [PATIENT SPECIMEN].

Note

The RBC count should be between 3.5 and 4.5. If the count is outside this range, modify the dilution ratio of the latex particles, exit Gain Adjust Mode and try again.

2. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE].

Press [SMOOTHING OFF].

Note

The key changes to [SMOOTHING ON] and is highlighted, indicating the smoothing function is ON.

[RBC HISTOGRAM].

Observe the lower part of the histogram data to determine if the channel number for peak of 100 is Channel 98 ± 1 channel.

If the specification is met, go to

RER Adjustment

If the specification is not met, adjust R27.

Note

Turning R27 clockwise decreases the channel number; turning R27 counterclockwise increases the channel number.

6. Return to the RUN screen and run another cycle.

Observe the channel number for peak count of 100.

If the gain is still outside of specification, repeat this process until the peak count meets the specification (100 is in Channel 98 ± 1 channel). Refer to

RBC Gain Adjustments (Example)

Note

Turning R27 clockwise decreases the channel number; turning R27 counterclockwise increases the channel number.

Go to

RER Adjustment

Continue with

RER Adjustment

RBC Gain Adjustments (Example)

RER Adjustment

Action

Determine

Adequate

Dilution Amount

Determine Edit

Ratio

Percentage

Steps

Verify that the dilution level on both the WBC and RBC transducers (left side) is above the aperture (orifice) plates.

If the dilution level in transducers is met, go to

Determine Edit Ratio

Percentage

If the specification is not met, go to

Step 2

2. From the RUN screen, press [SPECIMEN

TYPE], then [PATIENT SPECIMEN].

3. Press the touch plate to run a cycle (exits Gain

Adjust Mode).

4. Pour 10 mL of diluent into the Pre-Mix Cup and add 1 drop of well-mixed 5.0 µm latex particle solution to the 5 mL of diluent already in the

RBC Mixing Chamber.

5. In the RUN screen, press [SPECIMEN TYPE].

Press Shift and # (simultaneously) to activate the Gain Adjust Mode.

Note

The message <PREPARING FOR GAIN ADJ.> displays and diluent in the Pre-Mix Cup is transferred to the WBC Mixing Chamber. When the preparation cycle is complete, the RUN screen displays.

Press the Touch Plate to run a cycle. Make a note of the RBC results.

Note

The RBC count should be between 3.5 and 4.5.

If the count is outside this range, adjust the dilution ratio of the latex particles by adding more diluent or latex to the RBC Mixing

Chamber.

2. Repeat two more times for a total of three (3) runs. Calculate the average of the three RBC counts.

Refer to

Cell Edit Table

, to find the target value for the following:

Reference

Use the average RBC calculated in

Step 2

to determine the Edit Ratio

Percentage that coincides with the calculated average.

Note

Example: A count of 4 million relates to an edit ratio of 28.0% ± 1.3.

Adjust RER

Note

Use the edit ratio calculated in Determine Edit

Ratio Percentage above to perform the following procedure:

Cell Edit Table

1. From the MAIN Menu, press [DIAGNOSTICS] followed by [RAW DATA].

Observe the RBC RER displayed on the screen from the last cycle.

If the specification is met (see

Cell Edit

Table

), go to

WBC Gain

If the specification is not met, go to

Step 3

Adjust R51.

Note

Turning R51 clockwise decreases the RER percentage; turning R51 counterclockwise increases the RER percentage.

4. Return to the MAIN Menu and run another cycle.

screen.

6. If the ratio is still outside of specification, repeat this process until the RBC Editing Ratio is within the specified value.

7. Return to the MAIN Menu and press [RUN] to access the RUN screen.

8. Observe the RBC Histogram.

The trailing edge of the histogram should be straight with almost no humps. Refer to

Editing

Ratio Displays

Continue with

WBC Gain

Editing Ratio Displays

Go to

WBC Gain

WBC Gain

Action Steps Reference

Prepare Latex

Dilution

Set WBC

Gain

[RUN] screen, press [SPECIMEN TYPE], then [PATIENT SPECIMEN].

2. Press the touch plate to run a cycle (exits Gain Adjust Mode).

3. Obtain a clean container.

4. From the MAIN Menu, press [SPECIAL PROTOCOLS] followed by [MORE] twice to display the [10 mL DISPENSE] key.

Press [10 mL DISPENSE] four times to dispense 20 mL of diluent into the container.

Note

Each 10 mL dispense requires key to be pressed twice.

6. Add 1 drop of well mixed 5.0 µm latex particle solution into the 20 mL of diluent and mix well.

7. Before entering the Gain Adjust Mode, use the prepared WBC latex dilution to fill the

Pre-Mix cup up to the level of the diluent inlet port.

8. From the MAIN Menu, press [RUN] followed by [SPECIMEN TYPE].

Press Shift and # (simultaneously) to activate the Gain Adjust Mode.

Note

The message <PREPARING FOR GAIN ADJ.> displays and WBC dilution is transferred to the WBC Mixing Chamber. When the preparation cycle is complete, the

RUN screen displays.

Press the touch plate to run a cycle.

Note

Always return to the RUN screen to run a cycle.

Note

When the procedure is completed, or if there is a need to exit the Gain Adjust Mode while performing this procedure, such as constant clogs or improper dilution ratio, press

[SPECIMEN TYPE] followed by [PATIENT SPECIMEN].

Note

The WBC count should be between 10.0 and 30.0. If the count is outside this range, modify the dilution ratio of the latex particles, exit Gain Adjust Mode and try again.

2. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE].

Press [SMOOTHING OFF].

Note

The key changes to [SMOOTHING ON] and is highlighted, indicating the smoothing function is ON.

[WBC HISTOGRAM].

Observe the lower part of the histogram data to determine if the channel number for peak of 100 is Channel 56 ± 1 channel.

If the specification is met, go to

PLT Gain

If the specification is not met, adjust R22, and repeat

Step 1

through

Step 5

Note

Turning R22 clockwise decreases the channel number; turning R22 counterclockwise increases the channel number.

Go to

PLT Gain

Continue with

PLT Gain

WBC Gain Adjustments

PLT Gain

Action

Prepare

Latex

Steps

then [PATIENT SPECIMEN].

Reference

Note

The PLT channel cannot be correctly

2. Press the touch plate to run a cycle (exits Gain Adjust

Mode).

From the MAIN Menu, press [SPECIAL

PROTOCOLS] followed by [MORE] twice. Place a clean container under the Sample Probe and press

[10 mL DISPENSE] four times to dispense 20 mL of diluent.

Note

Each 10 mL dispense requires key to be pressed twice.

4. Add one (1) drop of well-mixed 3.31 µm latex particle solution and mix well.

5. Hold the diluted latex solution under the Sample Probe and press [1/50 DILUTION] to aspirate for 1:50 dilution, while in the SPECIAL PROTOCOLS Menu.

6. Place a clean container under the Sample Probe and press [1/50 DISPENSE] to dispense the 1:50 dilution.

7. Pull open Solenoid 2-5 (located directly under the left side of the RBC/PLT Transducer) to drain the existing solution in the RBC Mixing Chamber.

Pour the 1:50 dilution of 3.31 latex particles into RBC

Mixing Chamber.

Enter Gain

Adjust

Mode

Adjust PLT

Gain

1. Pour 10 mL of diluent into the Pre-Mix Cup (do not add any latex particle solution).

2. From the RUN screen, press [SPECIMEN TYPE].

Press Shift and # (simultaneously) to activate the

Gain Adjust Mode.

Note

The message <PREPARING FOR GAIN ADJ.> displays and WBC dilution is transferred to the WBC

Mixing Chamber. When the preparation cycle is complete, the RUN screen displays.

Note

When running PLT counts, the lower front cover must be on the instrument to prevent noise from interfering with PLT results.

Press the touch plate to run a cycle.

Note adjusted unless the RBC channel is at the correct gain. The RBC channel must be verified prior to adjusting the

PLT channel.

The PLT count should be between 150 and 350. If the count is outside this range, adjust the dilution ratio of the latex particles by adding more dilution or latex.

2. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE].

Press [SMOOTHING OFF].

Note

The key changes to [SMOOTHING ON] and is highlighted, indicating the smoothing function is ON.

[PLT HISTOGRAM].

Observe the lower part of the histogram data to determine if the channel number for peak of 100 is

Channel 136 ± 1 channel (refer to

PLT Gain

Adjustments (Example)

.).

If the specification is met, the procedure is complete.

If the specification is not met, adjust R41.

Note

Turning R41 clockwise decreases the channel number; turning R41 counterclockwise increases the channel number.

6. Return to the RUN screen and run another cycle.

7. Repeat through

Step 5

8. To exit Gain Adjust mode, go to the RUN Menu and press [SPECIMEN TYPE] followed by [PATIENT

SPECIMEN]

9. Run two to three background cycles.

10.

Verify acceptable background results (refer to

Specifications & Checklist

PLT Gain Adjustments (Example)

VP-10 Diluent and Sample Verification/Adjustment

Purpose

Materials Required

Verify diluent dispense to maintain proper dilution ratios. Module CELL-DYN 1800

10 mL Graduated Cylinder

40 µL Micropipettes

100 µL Micropipettes

Clean 50 mL Container

Time

00:15 min

WARNING

Potential Biohazard

This procedure consist of both Diluent and Sample adjustment that can be accessed with the following links:

Diluent Volume Verification

Sample Volume Verification

To minimize problems like coincidence passage, the CELL-DYN 1800 uses two different dilution ratios of whole blood to diluent. The ratio for WBC/HGB is 1:285; the ratio for RBC/MCV/PLT is 1:12,801. The following procedure is used to verify the diluent dispense to maintain proper dilution ratios and thereby optimize instrument performance.

Diluent Volume Verification

Action

Verify

Diluent

Volume

Steps

1. From the MAIN Menu, press [SPECIAL PROTOCOLS] followed by [MORE] twice.

2. Place an empty 10 mL graduated cylinder under the Sample Probe and press [10 mL

DISPENSE] twice to dispense 10 mL of diluent.

3. Verify a volume of 10 mL ± 0.2 mL.

4. Place an empty 10 mL graduated cylinder under Sample Probe and press [1/50

DILUTION]. Once the probe has returned to the aspirate position, press

[1/50 DISPENSE] to dispense.

Verify a volume of 5 mL ± 0.1 mL.

Reference

Note

The volume dispensed is under direct computer control. If the volume is out-of-range, the

Dispenser and Stepper Motor drive circuitry must be repaired. (Refer to RR-E1.02

Diluent Syringe Driver Assembly .)

Sample Volume Verification

Action

Sample Volume

Verification

Steps

1. Remove the 1/32" silicone tubing attached to the top of the sample probe.

2. Attach a 100 microliter pipette to the silicone tubing.

Place the tip of the pipette on the bottom of a small container and press [10 mL

DISPENSE] twice.

Note

Keep the tip of the pipette submerged when dispensing.

Reference

4. Take the pipette out of the container and wipe any drop from the end of the pipette, being careful not to wick any liquid from the end.

[1/50 DILUTION].

6. Verify that the column of liquid is no more than 1/16 inch above or below 100 microliter mark on the pipette.

7. Replace the 100 microliter pipette with a 40 microliter pipette.

8. Place the tip of the 40 microliter pipette on the bottom of a small container and press

[1/50 DISPENSE].

9. Take the pipette out of the container and wipe any drop from the end of the pipette, being careful not to wick any liquid from the end.

10. Press [1/250 DILUTION].

11.

Verify that the column of liquid is no more than 1/16" above or below 40 microliter mark on the pipette.

Remove Pipette

1. Place a waste container under the tip of the pipette and press [1/250 DISPENSE].

Remove the pipette and re-attach the silicone tubing to the sample probe.

Note

The volume aspirated is under direct computer control. If the volume is out-of-range, the Sample Syringe and Stepper Motor Drive must be repaired. (Refer to RR-E1.04

Sample Syringe Driver Assembly .)

VP-11 Stepper Motor Power Test and Verification

Purpose

Verify stepper motor power.

Module CELL-DYN 1800

Materials Required

None

Time

00:30 min

Action

Test Stepper

Motor Power

Steps

1. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times.

Then press [SERVICE DEC CODE].

128 from the keyboard and press Enter.

3. The test runs for approximately 30 seconds.

4. When the test is complete, the results are displayed on the screen.

Compare the results displayed on the screen with the nominal values listed in

Motor

Reference

Power Specifications

. Ensure that all values fall within the specified ranges.

Motor Power Specifications

VP-12 Sample Probe Alignment Check

Purpose

Check Probe Alignment.

Module CELL-DYN 1800

Materials Required

Standard Set of Hex Wrenches Time 00:10 min

Action

Check

Probe

Alignment

Steps

1. From the MAIN Menu, press

[DIAGNOSTICS], followed by [MORE] twice, then [PROBE HOME].

Observe the wash block in relationship to the probe tip. Verify that the tip of the probe is between the waste removal channel and the top of the countersink, located at the bottom of the wash block.

Note

Proper probe tip location is essential for proper cleaning of the probe, and prevents carryover and CV problems.

3. If alignment is required, loosen the set screw on the probe collar and raise or lower probe until proper height is

Reference

achieved.

4. Retighten set screw on probe collar.

Press PROBE DOWN to exit.

VP-13 Serial Transmit to LIS Verification

Purpose

Verify operation of serial port.

Module

CELL-DYN 1800

Materials Required

Laptop computer w/Windows 2000

Copy of CELL-DYN 1800 Interface Specification (Latest Revision)

Nine (9) Pin Null Modem Serial Cable

Time

00:40 min

This verification procedure is used to verify the operation of the serial port (COM 1) that is used for communication with a

Laboratory Information System (LIS). This procedure only verifies the transmit function.

A 9 pin, "null modem", serial cable is needed for this procedure.

Note

The screens shown in this procedure are given for example only. Each model instrument has slightly different field structure and content to the count data message that is downloaded. Consult the applicable interface specification document for details.

Action

Create New

Connection

Steps

1. Place the laptop near the instrument and connect the null modem serial cable to

COM 1 on the right side of the instrument.

Reference

Connect the other end to COM 1 on the laptop.

2. From the Windows 2000 desktop, use the mouse to click on Start.

Highlight Programs, Accessories,

Communications, and click Hyper

Terminal.

The Connection Description window displays.

sertest1.

Select the first icon and click the OK button.

Select

Connection

Connect To window displays:

At the bottom, in the box Connect

using:, use the mouse to select COM 1.

Note

All the other boxes are blocked (gray).

Click the OK button.

Configure

COM1

COM1 Properties window displays: match the Baud Rate in the CELL-DYN

SETUP menu.

3. Verify that the Data bits, Parity and Stop

bits are configured according to the

CELL-DYN SETUP menu.

In the CELL-DYN SETUP menu, verify

the CTS enabled setting: o

If CTS enabled is off set Flow

control: to Xon/Xoff.

If CTS enabled is on set Flow

control: to Hardware.

Click the OK button.

sertest1

Active

Transmit

Data

The test session window named sertest1 is now active.

Note

If the message "Unable to open COM1.

Please check your port settings" displays, there may be another device assigned to the COM1 port, such as the modem. Contact the local area IS administrator for assistance with resolving device interrupt conflicts.

Compare the results to the interface specification. The screen is shown for example only. Transmit specimen data as many times as desired.

1. From the MAIN Menu, press

[DATALOG], followed by [TRANSMIT

DATA].

2. Enter starting and ending sequence numbers of data to be transmitted.

Compare the results to the

CELL-DYN 1800 Interface Specification.

Note

The screen below is shown for example only.

If desired, click on File then Print to obtain a printout. When testing is complete, click on File then Save. After saving, click on Call, then Disconnect, then exit the window by clicking the X.

Close out the Hyper Terminal window by clicking the X.

VP-14 Hard Disk Drive Setup and Verification

Purpose

Materials

Required

Provide instructions to verify/modify CMOS Settings, partition and format replacement hard disk drives

DK, Drive, Hard, IDE (8200570701)

DKS (C) SRV ABBOTT CD3200 (8938070001)

CELL-DYN 1800 Installation Disk

Phillips Screwdriver

Action

Preparation

Module

CELL-DYN 1800

Time

00:30 min

Steps

Note

If replacing an existing customer hard disk drive, print copies of all customer settings, quality control data files, and the data log if the drive is accessible. It may not be possible to access this data if the hard disk drive is corrupt or defective.

Reference

OFF the system.

Verify jumper setting is set to Master according to Jumper Configuration Table on hard disk drive.

Install Hard Drive

Install the new hard drive using the existing hardware and cables (refer to

RR-F1.04 Hard Disk Drive

Access CMOS Setup

Utility Screen

Power ON the analyzer and press the Delete key, when prompted, to display the CMOS Setup Utility Screen.

Verify CMOS Settings:

Standard CMOS Setup

1. Use the up/down arrow keys to select the STANDARD CMOS SETUP.

Enter key.

Pri Master to Auto.

Set 32 Bit Mode to ON.

Note

Pri Slave, Sec Master and Sec Slave show Not Installed.

Press ESC to exit the screen.

Verify CMOS Settings:

Advanced CMOS Setup

1. Use the up/down arrow keys to select the ADVANCED CMOS SETUP.

Enter key.

Quick Boot to Enabled.

1st Boot Device to Floppy.

2nd Boot Device to IDE-0.

3rd Boot Device to Disabled.

4th Boot Device to Disabled.

Press ESC to exit the screen.

Verify CMOS Settings:

Advanced Chipset

Setup

Verify CMOS Settings:

Power Management

Setup

1. Use the up/down arrow keys to select the ADVANCED CHIPSET SETUP.

Enter key.

LCD CRT Selection to Simultaneous.

LCD Type to Type 8.

Press ESC to exit the screen.

1. Use the up/down arrow keys to select the POWER MANAGEMENT SETUP.

Enter key.

Power Management/APM to Disabled.

CPU Critical Temperature to 60°C/140°F.

Press ESC to exit the screen.

Verify CMOS Settings:

Peripheral Setup

Save CMOS Settings and Exit CMOS Setup

1. Use the up/down arrow keys to select the PERIPHERAL SETUP.

Enter key.

OnBoard FDC to Enabled.

Press ESC to exit the screen.

1. Place the DKS (C) SRV ABBOTT CD3200 (8938070001) (service disk) into the floppy drive.

Create DOS Partition

Format Hard Drive

Press F10.

The message Save current settings and exit (Y/N)? displays.

Type Y and press Enter.

Note

Saves the CMOS settings, exits the CMOS Setup Utility and restarts the computer.

A:\> prompt, type FDISK and press Enter.

FDISK Options menu, enter choice 1 and press Enter.

At the Create DOS Partition or Logical DOS Drive menu enter choice 1 and press Enter.

Note

If you get the message Primary DOS Partition already exists, press Esc to exit. Then go to Format Hard Drive , Step 1 .

The message Do you wish to use the maximum available size for a

Primary DOS Partition and make the partition active (Y/N)? displays.

Type Y, and press Enter.

Note

Uses the maximum size for the hard drive DOS partition and makes it active.

When the screen displays System will now restart. Insert DOS system

diskette in drive A:. Press any key when ready, press Enter.

The system reboots and displays the A:\ prompt.

At the A:\> prompt, type FORMAT C: /S and press Enter.

The message WARNING: ALL DATA ON NON-REMOVABLE DISK DRIVE

C: WILL BE LOST! Proceed with Format (Y/N)? displays.

Type Y, and press Enter.

The message Volume label (11 characters, ENTER for none)? displays.

Press Enter for None.

Install DOS and System

Software

At the A:\> prompt, type INSTALL and press Enter.

Note

Verification

Installs DOS and system files.

2. When the system installation is complete, remove the service disk and insert the CELL-DYN 1800 Installation disk into the floppy drive.

Type Install and press Enter.

Note

When the installation is complete, the message "Power off the instrument

and remove diskette. Power on the instrument." displays.

Remove the CELL-DYN 1800 Installation disk and power OFF the instrument.

Verify that the system boots up properly to the application software.

Note

During initial power up after a hard disk drive installation, DO NOT turn off the instrument while the Data Log is being created. This corrupts the Data Log.

If printouts are available, re-enter the data from the printouts. If not, perform instrument calibration procedure and verify control recovery.

VP-15 Software Installation/Upgrades

Purpose

Install or Upgrade Software Module CELL-DYN 1800

Materials Required

Disk, installation (8938144001) Time 00:10 min

Software

Action

Installation/Upgrade

Steps

1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.

C:\CD1800>.

4. Insert the CELL-DYN 1800 Installation disk into Drive A.

a: and press Enter.

Reference

Type install and press Enter.

Note

When the installation is complete, the message "Power off the instrument

and remove diskette. Power on the instrument." displays.

7. Remove the diskette and power OFF the instrument.

Power ON the instrument and allow it to initialize.

Note

During initial power up after a hard disk drive installation, DO NOT turn off the instrument while the Data Log is being created. This corrupts the Data Log.

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Key features

Automated Hematology Analyzer
Flow Panel System
Fluid Power Supply
Reagent Inlet Panel
Syringe Drive Assemblies
Electronics Card Cage
LCD Display System
Touchpad (Membrane) Keyboard
Power Supply Assembly
Service and Support Manual

Frequently asked questions

The ABBOTT CELL-DYN 1800 is a complex system that relies on several components to perform hematology analysis.

The ABBOTT CELL-DYN 1800 system consists of 8 components and subsystems, including Flow Panel System, Fluid Power Supply, Reagent Inlet Panel, Syringe Drive Assemblies, Electronics Card Cage, LCD Display System, Touchpad (Membrane) Keyboard, and Power Supply Assembly.

The Flow Panel System is responsible for sample aspiration, dilution, measurement, and waste removal.

The Fluid Power Supply contains vacuum and pressure pumps, accumulators, waste bottles, and associated solenoids with hardware.

The Reagent Inlet Panel provides connections for incoming reagents and outgoing waste. It also houses the Lyse solenoid.

ABBOTT

CELL-DYN 1800

Automated Hematology Analyzer

Service & Support Manual

Theory of Operation

Troubleshooting

Engineering Drawings and Schematics

CELL-DYN 1800 PCB Reference

Removal & Replacement

Covers (CD18-A1)

Flow Panel (CD18-B1)

Fluid Power Supply (CD18-C1)

Syringe Assembly (CD18-E1)

Electronics / Card Cage (CD18-F1)

LCD/Keyboard (CD18-G1)

Verification Procedures

VP-01 Preparation for Alignment and Verification

What is the ABBOTT CELL-DYN 1800 Automated Hematology Analyzer?

What are the components and subsystems of the ABBOTT CELL-DYN 1800?

What is the purpose of the Flow Panel System in the ABBOTT CELL-DYN 1800?

What does the Fluid Power Supply in the ABBOTT CELL-DYN 1800 contain?

What is the function of the Reagent Inlet Panel in the ABBOTT CELL-DYN 1800?

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