SPECIFICATION FOR ULTRA CENTRIFUGE Item No. 1 Working Voltage == 220-230 Volts RPM / g – 100,000 RPM / 800,000g Easy Protocol Set up; Low Noise Level. LCD-Touch Screen Display. Rotar Tracking Facility ; Password Protected ; User Defined Programme Temperature 00 - 400.C Speed Control +/- 10RPM – Rotor Requirements : (a) (b) Angular Swing out with compatible tubes and adaptors. (Please Quote Different Rotors with price individually Equipment should be supplied with two AC of 1.5 ton capacity) Stabilizer : Good Quality Compatible and Robust. 5 Year Warranty / Guarantee for the main equipment. User Lists and Other Detail Accessories: Different types of tubes/adaptors compatible to the rotors may be quoted with price mention separately for each item. Item No. 2 Server Server specificationIntel Xeon Processor E5 2630(6 Core) 2.3 GHz/1333 MHz/ 15 MB Memory 4 x 8 GB, HDD 3 x 300 GB SAS HDD, 2.5” Hot Swap, Multi Burner, RAID 05 in built (M5110 e card + 512 MB Cache) + RPS x 2 HS Component: Power Supply, Fan module, HDD Storage Bays: 16 x 2.5” hot-swap drive bays or 8 x 2.5” hot-swap drive bays 1 x media bay Intel USB port, I/O Slots: Riser Slot , IX PCI Express 3.00 x 8 (FH./FL)+ 2 x PCI Express 3.00 x 8 (HF/FL) , IX PCI Express 3.00 x 16 (FH/FL) + 1 x PCI Express 3.00 x 8 (HF/FL), IX PCI-X(FH/FL) + 1 x PCI –X(FH/FL) + PCI Express 3.00 x 16 (HF/FL) with Key Board, Mouse and 18.5” Monitor rack with complete accessory with three years onsite warranty. Item No. 3 Specifications for Nanodrop 1. LCD Display 2. Wavelength range from 220 onwards upto 900 nm or more 3. Sample size less than 3 microliter 4. Wavelength accuracy 1nm 5. Measurement cycle time should be less than 10 seconds 6. Electrical requirement: 220-240 volts, single phase, 50Hz to suit local conditions 7. Data output can be obtained via a printer and desktop with (Intel i5 processor) should be provided for data storage. 8. The specifications should be supported with original quoted model and its original broacher. 9. 2 Years Warranty with on-site service as and when required 10. Should accompany with compatible voltage stabilizer Item No. 4 Thermo mixer with 1.5ml Exchangeable block Specifications 1. 2. 3. 4. 5. System should be able to provide peltier driven heating and cooling, with mixing. Temperature range: 15° below ambient to 100°C, with minimum setting of 1°C Sample block design should be easily removable for cleaning or replacement. Mixing frequency: 300-3000rpm Instrument should be specifically designed to take care of cross contamination, by making sure spillage or lid wetting does not happen even at very low volumes 6. Timing range from 1min to 99h 7. Block Temp.accuracy and uniformity should be less than 10 C 8. Heating Time required should be less than 15 min (from 20 0C to 100 0C) 9. A heated top should be optionally available to prevent condensation on the lid or walls of the tube. 10. Instrument should be able to recognize the blocks automatically and their respective speed and temperature. 11. Soft keys for easy access to program parameters should be present 12. System should be stable on work bench even at the maximum speed with minimum noise levels 13. The specifications of offered thermo mixer model should be supported by original broacher. 14. Warranty period minimum 2 years Accessories should include 1. Thermo block of atleast 24 micro tubes in capacity of 1.5 ml. 2. Thermo block should be of atleast 48 micro tubes in capacity of 0.5 ml. 3. A voltage stabilizer from a reputed firm Item No. 5 REFRIGERATED CENTRIFUGE Details of specifications 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Fully functional table top refrigerated centrifuge with rotors Microprocessor controlled, Broad LCD display Pre-cooling of rotor and chamber function CFC free refrigeration system Pulse/short run/ continuous run option and timer settings o o Temperature set range -10 C to 40 C Maximum speed 15000 rpm or more Maximum g-Force/RCF 25000 g or more Maximum volume 300 ml or more at a speed of ≥14000 RPM and ≥20000 RCF Sound level <66 decibles Safety features i. Display of malfunctions like lid open, rotor imbalance, over speed, temperature shoot, abnormal rotor mounting. Power fluctuations etc. ii. Rotor identification and error display 12. Rotors to be supplied essentially with machine (Prices of each item to be mentioned separately) i. Fixed angle rotor for 24/30 X 1.5/2.0 ml tubes : max speed ≥ 15000 rpm & ≥ 25000g ii. Adaptor for 0.2ml tubes (30no.) compatible for fixed angle rotor iii. Fixed angle rotor for 6/8 X 50 ml tubes : max speed ≥ 14000 rpm & ≥ 20000g iv. Adaptor for 15ml tubes (6/8no.) compatible for fixed angle rotor 13. Compatible servo stabilizer must be supplied essentially with the machine 14. Warranty on the machine and its essential accessories for a period of two years from date of installation must be provided and AMC charges for additional three years must be given separately. 15. Indian user list (more than 10) with contact no. and telephone no should be provided. 16. Technical features described above should be supported by the authentic original company catalogue of the quoted instrument. 17. Vendor should provide income tax return for at least 3 previous years. Item No. 6 Analytical electronic weighing balance Weighing cap. -- 200-230 g Readability --0.1 mg Repeatability ---- ± 0.1 mg Linearity --- ± 0.2 mg Pan Size ---- 80- 100 mm With stabilizer 0.5 KVa Three year warrantee Desirable: 1.List of govt organizations supplied 2.Company Catalogoue Item No. 7 ELISA Reader along with colored printer Detailed specification; ● Wave length range 400-1000 nm ● Photometric range 0.0-4.0 OD ● Filters 8 position filter wheel ● Linearity ≤1.0% from 0.0-2.0 OD; ≤2.0% from 2.0-3.0 OD ● Accuracy ± 1.0% or 0.010 from 0.000-3.000 OD at 490 nm ● Precision ± 1.0% or 0.005 OD from 0.0-2.0 OD; ● Resolution 0.001 OD Light source Tungsten halogen lamp average life hr. 3000 hr. ● Plate Types 96 well plate, plate height 16 mm. with flat, U, V shape bottom ● Linear shaking, built in shaker, three speed (low, medium & high) ● Duration second 0.999 Stability & drift < 0.10 OD at OD = 1 at 490 nm single wave length ● Read time 6 seconds at single wavelength, 10 seconds at dual wavelength ●Data output onboard graphical thermal printer and USB 2 interface Equipment should be stand alone ● Data storage Calender / clock functions; 64 assay protocols ● print out report supported ● Motorized support for plate loading and retrieval ● Software; High throughout analysis and reporting, Comprehensive Curve- Fit Analysis, complex Kinetic Analysis ● 240 Volt, 50/60 Hz, Stabilizer 0.5 Kva Optional- Coloured printer ● Three years warrantee; Three years service contract Bid should be supported with company catalogoue and list of research organizations supplied Item No. 8 Phase contrast Microscope with photo image device and thermostate. Specifications: Eye piece 10 X (Two pcs) with Diopter and eye guard, antifungal treated. Plan achromatic objectives 10X, 20X, 40X & 100 X (oil), Course and fine adjustment. Sextuple revolving nose piece with slot for POL/DIC slider. Condenser for bright field, phase contrast and dark field microscopy or better. Illumination 12V, 100 W Halogen lamp / LED lamp with inbuilt filters. Mechanical stage, ceramic coated. Digital controlled stage warmer (ambient to 500 C) device with uniform distribution system. Digital Camera at least 5 mega pixel. Soft ware for capturing images, storage, image enhancement and measurement. Computer branded processor i 7, 2GB RAM, DVD Writer, 320-500 GB or higher HDD, 17-18 inches TFT monitor along with UPS, fire wire, optical mouse and key board. Microscope & Camera compatable should be from same manufacturer. Accessories: (optional); Halogen bulb- Two; Eye piece 10 X-one; Objectives 40 X, focusing telescope for phase contrast light, Immersion oil. Item No. 9 Specification For Somatic cell Counter/Analyzer Light Weight, portable (5-10Kg) Analysis Time: <1min. per sample Capacity: min.30 sample per hour Measurement rang: min 10,000 upper limit; 30, 00,000 somatic cells/ml Operating Temp.: 10 to 40°C Sample temp.: 20°C to 60°C Milk sample volume: 50 to 75µl in cassette Repeatability: 7 to 10% Power supply: 220v/50Hz Minimum 1 year guarantee after installation on all parts. The instrument should also work without computer Facility for data transfer to an external Pc/Printer using USB connection The tender should come with original brochure. Optional: Rate for AMC for 5 years after the warranty period. Cell counter win software and database kit for PC connectivity. Cell counter will come in a bag with Battery. 2.0 KVA UPS of reputed make with batteries for minimum30 min back up. This to declare that the specification list above, are generic in natures & does not reflect to any particular brand in manufacture. Optional: Additional cassettes for 1000 samples will be required. : Rate for AMC for 5 years after the warranty period. ELISA Reader -Monochromator-Based Reader with following specifications 10 Detection mode UV-Visible Absorbance Read method End-point, kinetic Microplate types 1- to 384-well plates (96 well and 384 well) Shaking Yes Software Data analysis software with Excel export Control through USB or serial port Automation Yes Wavelength selection Monochromator Wavelength range 230 - 900 nm, 1 nm increment Bandpass 4 nm (230-285 m), 8 nm (>285 m) Dynamic range 0 - 4.0 OD Resolution 0.0001 OD, 0.0010D Monochromator wavelength accuracy +2 nm Monochromator wavelength repeatability +0.2 nm OD accuracy <1% at 2.0 OD typical <3% at 3.0 OD typical Temperature control 4°C above ambient to 45°C (+0.5°C) User Interface On-board as well as PC control and support UPS, dust covers and other required accessories Item No. Item No. 11 Biological microscope with digital camera with following specification: 1. Objective Lenses: quadruple objective revolving nosepiece. Plan Achromat 4x, 10x, 40x, 100x oil objectives. 2. Eyepieces: high eye point 10x eyepieces 3. Total Magnification: 100 - 1000x. 4. Focusable condenser 5. Focusing: Fine and coarse coaxial focusing. 6. Halogen illumination system. 7. With compatible digital camera. Specifications of Gradient PCR machine with UPS Gradient Block to accommodate 96 x 0.2 ml tubes / PCR plate / PCR strips Minimum sample working volume : 10ul Temperature control of the block : 4-99oC Gradient Block over columns so that 12 temperature gradients can be run simultaneously Gradient selectable from 1oC to 24 oC across the entire temperature control range Gradient temperature range 30-99 oC Auto restart option in the event of power failure Ability to store methods on external card for safety and multiple user capability Should have memory to store at least 800 protocols. Should have heated lid system Temperature Uniformity : ± 0.5 oC Temperature Accuracy ± 0.25 oC Maximum block ramp rate ≥ 4.25 Display : Large display of set parameters showing real-time assay conditions with touch screen Two years warranty on the machine. Compatible online UPS with at least 30 minute power backup Primer designing and oligo analysis software should be quoted as optional Original catalogue must be provided Item No. 12 Item No. 13 Specifications of Gel Documentation System A high performance and easy to use digital imaging system for fluorescent and colorimetric imaging for DNA, RNA and Protein gels equipped with 1. Dual-Wavelength Transilluminator with High and low intensity settings to view the DNA/RNA gels 2. The transilluminator must have provision to slide in and out of cabinet as required 3. White light source for colorimetric applications such as Coomassie gels other colorimetric assays 4. Imaging area of at least 20 x 25 cm. 5. Should support fluorescent and colorimetric detection methods based on dyes such as Ethidium bromide, Coomassie Blue, Silver Stain, SYBR Gold, SYBR Green, Hoechst Blue, SYPRO Red, Fluorescein and GFP 6. Should have metallic darkroom hood for chemiluminescence/fluorescence imaging. 7. High-resolution 12-bit CCD Camera with USB 2.0 computer interface and at least 1.4 Megapixel Resolution. 8. Should be capable of automatically correcting imaging artifacts 9. Should provide real-time imaging and analysis within seconds 10. Should have software controlled motorized lens to allows direct control of aperture, zoom and focus. 11. Should have auto focus technology with pre-calibrated focus for any zoom setting or sample height so that images are in focus at all times, regardless of zoom level or sample position. 12. Should have atleast 3 modes illumination control i.e., Trans-UV, Trans-white and Epi-white and at least 3-position filter holder 13. Should have software for image acquisition, documentation, and analysis including features like densitometry, automated quantitative analysis of samples etc. 14. Data processing unit comprising desktop PC with a latest processor, TFT monitor at least 19” size, 3 GB RAM and 320 GB hard disk, DVD writer and preloaded with latest operating system software. 15. Compatible UPS with at least 30 minutes backup Original catalogue must be provided Item No. 14 Specification: Bench top incubator shaker Bench top incubator shaker with temperature control range from 7°C above ambient to 50°C with accuracy ±0.1°C. It should have orbital shaking. Speed should be 50-350 rpm with accuracy of ±2rpm It should have acceleration/deceleration circuit to prevent sudden starts/stops. It should have timer up to 99 Hrs. It should display speed, running time, alarm etc on display screen. It should have alarms alert when speed or temperature deviate from set points. The system should have port for data logging. It should have automatic restart after power interruption. It should stop agitation when chamber is open. It should have universal platform and clamps to holds various flask. It should be supplied with compatible stabilizer. Operational voltage should be 220±20V Should have two years standard warranty/from the date of installation Should quote for the price of CMC/AMC per year after the expiry of standard warranty. Should provide user list with phone no and e mail ID Should provide brochure in original. Item No. 15 Specifications for mini SDS and western blot apparatus assembly Mini SDS-PAGE Should have the capacity to hold at least 10-wells, gel of 1.0 mm thickness and should hold a gel size of approximately 8 cm x7 cm. Should have integrated spacers with glass plates for ease of casting. Should include atleast 5 sets of compatible glass plates (spacer plate and shorter plates), a casting stand and 2 casting frames. Should include all the accessories of SDS-PAGE gel including sample loading guide, electrode assembly, tank, lid with power cables and mini cell buffer dam. Should be a modular system to support blotting and electro-elutions also in the same system. Powerpack Should have output range of 10-300 V and 4-400 mA, fully adjustable. Should have power of approx. 75W and time control of 001-999 minutes, fully adjustable. Should have atleast 4 pairs of output jacks. Operational voltage should be 220±20V The power supply should be capable to operate four electrophoresis units simultaneously for identical runs with LED display. Type of out put – constant voltage and constant current Auto power up feature after power failure should be there. Western Blotting System Should hold the gel size of upto 7.5x10 cm. Should be compatible with the mini-SDS-PAGE gel apparatus. Should include all the accessories required for Western blotting including buffer tank, buffer cooling unit, electrode module, gel holder cassette, fiber pad and lid with power cables. Should have the capacity to run 2 mini gels simultaneously. Should come with Color coded cassettes and electrodes to ensure proper orientation. Should come with cooling unit. Should be a modular system. Should provide user list with valid phone no and e mail ID Should provide brochure in original. Should provide operational voltage 220±20V Item No. 16 Deep Freeze (-80°C) Specification Minimum capacity of 360 litres. Vertical/ upright model. Should have at least 4 stainless steel shelves/ compartments. Digital front mounted microprocessor control panel. Preferably vacuum insulation. Single handed door latch with lock facility. Heavy duty low noise compressor. CFC-free and HCFC-free refrigerants. Should have alarm for temperature, filter clean, power out, system fail Must have auto restart function after power failure Warranty for 2 years after installation. Instrument should be CE and UL/ CSA certified. Air conditioner (1.5 ton) should be provided with the equipment. Item No. 17 1. Specifications for Ultra Low Freezer (–80ºC) 1. Fully programmable microprocessor controlled VERTICLE (upright) type freezer with the minimum capacity of approximately 100 L. 2. Freezer temperature should be below –80ºC at room temp up to 32ºC. 3. Stage compressors working in a cascade for maximum heat removal and quick recovery to set temperature with at least 1°C increment at room temp of 32ºC. 4. CFC-FREE, HCFC-FREE non flammable refrigerant compressor, should be capable to run on any voltage between 190-270 V. 5. Freezer should have at least TWO independent lockable compartments with inner doors and adjustable height stainless steel shelves to hold at least SIX racks and 60 boxes of 2” in each freezer. 6. System should have high grade stainless steel interior and tough, powder coated exterior finish constructed on steel gauge. 7. Ultra thin vacuum insulation panel and silicon gasket on the doors for tight sealing to avoid formation of ice crystals and to reduce temperature recovery time after door opening. 8. Audible and visible security alarms-for deviation from set temperature, power failure, probe failure, system failure, clean filter and door open, etc. 9. Eye level display of temperature parameters should be there. 10. Freezer must have ISO 9001-safety requirements and IEC 61010 Electrical safety CE certified. 11. At least TWO years onsite warranty for complete equipment, TEN years warranty on Insulation Panel and FIVE years warranty on compressor should be provided. 12. Freezer should be supplied with 3KV Compatible Servo voltage stabilizer with TIME DELAY FACILITY to restart after power failure. 13. INDIAN USERS LIST with contact address and telephone no. should be provided. 14. Technical features described above should exclusively be supported by authentic original illustrated company catalog of the instrument/equipment quoted. 15. Vendor’s business record is to be authenticated by producing Income Tax Return for at least 3 previous years. Optional For smooth functioning, 1.5 ton split air conditioner to control ambient temperature should be quoted additionally. Item No. 18 Chhana/Paneer preparation vat Milk loading capacity: 40-60kg Made up of high quality SS-304 Jacketed with provision of hot water circulation Completely equipped with agitator, knife and other attachments Digital temperature indicator. Provision for controlling the temperature Inbuilt heating system Complete fittings and installation Warranty: Two years guarantee/warranty Photograph/diagramme: photograph of equipment along with all the detail specifications should be supplied. User list: List of users with address, valid phone numbers and email Ids. Item No. 19 Specifications for Electrophoresis unit- Vertical as well as Horizontal along with power supply-1 set Double vertical Protein Electrophoresis system complete with: Two gel running capacity Gel Casting system, no tapes or grease Detachable electrodes Gel size of ~8 x 8 cm with two notched plates and two spacer plates with 0.75 mm, 1 mm and 1.5 mm spacer, comb with 10 wells and 15 wells of 0.75 mm, 1.0 mm and 1.5 mm thickness (two each) Horizontal Electrophoresis system with: Single cast mould to prevent leakage Tape free gel caster along with casting gates and leveller Detachable colour coded electrodes Midi:Gel tank, capable of accommodating 13 x 13 cm, 8 x 10, 5 x 8 with combs having 5, 10, 15, 13, 26 wells of 1.0 mm thickness and 3, 13, 26 wells of 1.5 mm thickness (Gel plates and combs of each type is required in pair) Maxi: Gel tank capable of accommodating 8 x 20 cm with 10 & 18 wells comb of 1.0 mm (two gel plates and 12 combs of each type are required) Power Supply with: 5-300 V in 1 V step 200W maximum Type of output: constant voltage or current with automatic crossover Output terminals: 4 recessed sets in parallel Timer: 0 - 999 minutes Safety features: No load detection, ground leak detection, sudden load change detection, overload/short circuit detection, auto power up after power failure. (Gel plates and the combs other than standard accessories should be quoted separately) Microscope with Digital Imaging facility Item No. 20 Specifications : 1. TRINOCULAR Microscope with Infinity Optical System, with fine & coarse focusing adjustment, anti-mold/anti-fungal treated interior optics. 2. EYEPIECE : A pair of wide field eyepieces 10x ( F.O.V : 20mm or better) with diapter adjustment in both the eyespiece. 3. NOSEPIECE : Quadruple revolving Nosepiece. 4. STAGE : Rectangular stage with specimen holder, stage should be provided with upper limit stopper. 5. OBJECTIVES : Plan Achromat 4x , 10X, 40X and 100X (oil) for phase contrast 6. CONDENSER : for Bright Field, Phase Contrast & Dark Field microscopy. 7. Microscope should be upgradable for Fluorescence microscopy. 8. ILLUMINATION SYSTEM : LED illumination with atleast 50,000 hrs life. 9. DIGITAL PHOTOGRAPHY system attachment (5 mega pixel CMOS camera) along with Image Analysis Software for arranging and capturing images, live preview, dynamic image analysis image measurement point to point basic report system etc. 10. DATA COLLECTION and processing unit with Processor Intel i5, 2 GB RAM, DVD Writer, 500 GB or higher HDD. 11. Suitable power stabilizer. Item No. 21 Food product Forming machine Automatic Machine for forming products of different shapes and sizes from the mixture of variable amount of milk/coagulum Forming of balls, nuggets, patties, biscuit like products etc. Moulds for different shapes and sizes Capacity to form 500 to 800 forms per hour Hopper capacity- 5 to 10 kg Facility to adjust weight of the products All food contact area of high quality SS Complete unit, easy to operate and clean Complete fittings and installation Warranty: Two years guarantee/warranty Photograph/diagramme: Photograph of equipment along with all the detail specifications should be supplied. User list: List of users with address, valid phone nos. and email Ids. Item No. 22 Specifications for Analytical Balance along with Stabilizer 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Built-in calibration mode with auto calibration on touch of a button Capacity 150-210g with 90-120mm pan size Tare range: Full to capacity Readability: 0.001mg Avereage response time: 5 sec or better Wind draft shield; side & top opening shielding windows for easy pan access Backlit digital display with sealed key pad Operating voltage: 220 - 240V Ambient conditions: 5 - 40°C; upto 85% humidity Dust protection cover Suitable voltage stabilizer Original illustrated catalogue to support technical specifications & Users’s list Two years on site warranty Optional: USB2/RS-232 port computer interface with easy data transfer to Windows®XP/7 OS or better based PC. Item No. 23 ELISA Reader with gradient filter cassette. Specification MEASURING RANGE : 0 TO 3.000 Abs. PHOTOMETRIC ACCURACY : +/- 1% or more RESOLUTION: 0.001 Abs. LIGHT SOURCE: Lamp Tungsten halogen with monochromator /Xenon with monochromator OPTICAL SYSTEM: Grating Monochromator WAVELENGTH RANGE: 370 nm to 850 nm standard continuously tunable DETECTOR : Silicon Photodiode DISPLAY: LCD INTERFACE TO PC: Software for bidirectional use POWER SUPPLY: 230 +/-10% VAC, 50 Hz Accessories: Halogen Lamps (Two) UPS: 2 KVa Warranty for 2 years after installation. Compatible desktop Computer with printer: and requisite software (s) for data reduction, multi-reduction, multilevel transformations, cut-off & validation formulas, flexible reporting options with sufficient data storage capacity and data output device. Item No. 24 Specifications for purchase of Server 1. A server with Mac Pro Two 2.4GHz processor 6-core Xeon/64GB/1TB/Radeon HD 5770 1GB/SD with protection plan 2. It should be supplied along with 1.5 ton split/window air conditioner unit with appropriate stabilizer in order to maintain ambient temperature while the equipment is functioning 3. Optional : Compatible UPS Gel documentation system Item No. 25 Specifications : 1. Gel documentation cabinet with 3 UV intensity (254, 302 and 356 nm) and white light illumination, with covered camera housing. 2. Rollout transilluminator tray 3. High resolution (2 mega pixel or better) CCD camera, 12 bit image capture, motorized zoom lens, 4. Gel viewing window with UV blocking shield . 5. Advanced image acquisition and analysis software. 6. Desktop PC (Processor Intel i5, 2 GB RAM, DVD Writer, 500 GB or higher HDD.) and LCD monitor (22 inch) from branded company 7. Laser Colour printer : 600 X 600 dpi resolution, colour print speed 8 ppm. 8. Compatible UPS Optional 1. UV torch (302 or 356 nm) 2. Thermal printer: 256 high resolution, print speed 1.9 sec. , print size 3“ X 4“ SPECIFICATION FOR INVERTED MICROSCOPE WITH PHASE CONTRAST AND BRIGHT FIELD MICROSCOPY ALONG WITH DIGITAL CAMERA SYSTEM Optical system Infinity-corrected optical system Revolving nosepiece Quadruple Stage Plain stage 160mm(L) x 250mm(W), stage clips attachable Attachable Mechanical stage with right-hand low drive coaxial controls, stage movement: X=120mm, Y=78mm, with Petri-dish holder, Terasaki- plate holder, and Slide glass holder, Stage extension plate, power cord Interference green contrast filter Interference light balance daylight filter Light source 6Volts 30Watt halogen lamp, built-in frosted and heat absorbing filters, detachable illuminator, Aperture diaphragm Trinocular Observation Head Detachable ultra-long working distance condenser (N.A. 0.3, W.D. 72mm) inclined 30°, interpupillary distance range: 48-75mm, tube length and diopter adjustment by helicoid on left sleeve Observation optical path: 50(binocular)/50(video port) Objectives Plan Achromat 4x ( N.A 0.10 W.D. 18.5) Condenser Plan Achromat Phase Contrast 10x ( N.A. 0.25 W.D. 10) Achromat Phase Contrast 20X ( N.A. 0.40 W.D.3.2) Achromat Phase Contrast 40X ( N.A. 0.55 W.D. 2.2) Plan Achromat 100x oil optional Eyepiece Wide field 10x. Field number 20 Spares Two nos. 6volts 30 watt halogen bulb Colour Digital Camera System Data Storage Device Digital SLR camera having 12 million pixel resolution or more. 1 GB or more storage card. AC adapter. Intel core i3 Processor , 3.0GHz, 4 GB RAM , 1000 GB HDD, 19” TFT Color Monitor, Windows 7, UPS MICROSCOPE AND CAMERA SHOULD BE OF THE SAME MANUFACTURER FOR BETTER COMPATIBILITY MICROSCOPE SHOULD BE UPGRADEABLE TO FLUORESCENCE AND RELIEF CONTRAST Item No. 26 Item No. 27 3D Perfusion Bioreactor S. No. 1 Features of specifications Cell culture chambers consists of four independent, autoclavable polycarbonate chambers that hold up to 10 scaffolds each. 2 With Peristaltic pump with minimum 6 channels 3 Aluminium base frame stand assembly for holding four chambers, four glass reservoirs, four small glass bottles. 4 Glass reservoirs for medium with caps and filters fittings: minimum four 5 Glass bottles with cap: Minimum four 7 Chamber stand: One 8 The chambers will be interchangeable and compatible with 3D Insert scaffolds ranging in size from 96-well to 6-well. Within each chamber there is a 1.5 mm minimum distance separating each scaffold. This distance, combined with the offset fiber configuration, ensures the perfusion of media. Chambers containing scaffolds can be easily removed and disassembled. The entire unit (except pump) is autoclavable and can be used as a single-use bioreactor system Warranty Period: Two years minimum 9 10 11 Other essential Items With required kits and Installation for smooth functioning of the instrument. One split AC (2 ton capacity): Reputed make, with 5 KVA Stabilizers, with installation Availability in the tender Item No. 28 Digital Weighing Balance (02 Units) 1. Monopan Balance with Glass partitions on all sides 2. Capacity : 300 to 320 GM 3. Readability : 0.1 MG 4. Repeatability : 0.2 MG or better 5. Linearity : 0.3MG or better 6. Average response time : 3 seconds or better 7. Tare Range : Full to capacity 8. Display : LCD with backlit display 9. Weighing pan: Three side triangular weighing pan with at least 80mm dia. 10. Weighing modes: In grams, milligrams kilograms, OZ, Ib 11. Selectable application programs: Mass unit conversion by toggling, tare memory, net total, weighing in percent, counting, averaging 12. Internal Calibration 13. Must have RS 232 port 14. Balance must be ISO Certified. 15. Warranty of at least TWO years must be provided 16. Suitable stabilizer to be Supplied Item No. 29 Specifications for Real Time PCR 1. Fully functional high throughput and fast Real-Time PCR system for qualitative and quantitative detection of nucleic acids, mutation screening and SNP analysis. 2. It should be an open system capable of running different chemistries using TaqMan, Molecular Beacon, SYBR green etc. 3. System should have 96 well block in plate format to accommodate 0.1/0.2 ml tubes/strips/plate. 4. System should support reaction volume less than 30 µl. 5. Temperature range of thermal block should be preferably 4 to 99°C and temperature accuracy should be about ±0.20 to 0.50° C. 6. System should support fast PCR protocol with ramp rate of preferably 4°C/sec or more. 7. System should be capable of multiplexing with at least 5 different fluorescent reporters. 8. System should be pre-calibrated for detecting at least five dyes (FAM/SYBR Green, VIC/JOE, TAMRA, Texas red & CY5 or similar wavelength fluorophores). 9. It should have an LED or Tungsten Halogen excitation source with either photodiode or CCD or PMT detector. 10. The software must allow analysis of multiple gene expression. The instrument software must be capable of detecting and analyzing a different gene, SNP or pathogen target in every well of the 96-well plate. Software should support performing Absolute quantification, Relative quantification, Allelic Discrimination, Plus/Minus assays. Along with this system should be able to conduct High Resolution Melting (HRM) experiments for genotyping and mutation scanning experiments. The HRM software must be provided essentially along with quoted system. 11. The vendor should have a functional laboratory and technical support team to support and troubleshoot problems. 12. System should be supplied with three years warranty on all part including excitation source (LED/lamp) and on-demand repair services as and when required. 13. Installation, testing, validation, demonstration/technical presentation should be conducted. The vendor should provide comprehensive training on the operation of the instrument, chemistry options and software. This training should be provided free of cost with all the consumables required for demonstration. 14. The equipment should be supplied along with 1.5 ton (5 STAR rating) split air-conditioning unit with appropriate stabilizer in order to maintain ambient temperature while the equipment is functioning. 15. The following accessories must be supplied with the equipment: Suitable on line UPS with a minimum 60 minutes power back up on full load. A desktop computer of a reputed make having following configuration: Processor-Intel Core i7 3rd gen., 4GB RAM, 500 GB HDD and at least 23 inch flat screen LCD/LED monitor and a color laser printer with a licensed Windows 7 professional OS and licensed original MS Office 2013. Start-up consumables (including plasticware & chemicals) for 1000 reactions. 16. Indian user’s list for the quoted models along with complete contact details including telephone no. & e. mail should be provided. 17. Technical features described above should exclusively be supported by authentic original illustrated company catalog of the instrument/equipment quoted. Photocopy or computer print-out of catalogue will not be accepted. 18. Vendor’s business record is to be authenticated by producing Income Tax return for at least 3 consecutive previous years. 19. Optional accessories: Spare light source (LED/Tungsten halogen lamp) should also be quoted separately as optional accessories. Item No. 30 Semi micro Balance Capacity and Readability = 0-40gm to 50gm x 0.01 mg Repeatability (Std deviation) - + 0.02 mg Response Time (avg) = 3 Seconds Tare Range = Full to Capacity Display = LCD Selectable weight Units = mg, gm Power consumption = supplied by AC adapter Sensitivity Drift (100 - 300C/500F-860F) = + 1 ppm/ 0C Allowable Ambient operating Temp = 00C to 400C, Built in RS-232 Interface = Yes Pan Size (3 Side Weighing pan) = 70 to 90 mm inner Diameter Warranty = 3 years manufacturer’s warranty SPECIFICATION FOR DOUBLE BEAM UV-VIS SPECTROPHOTOMETER Optical system Spectral Bandwidth Light Source : : : Double Beam 1.0 nm Tungsten – Deuterium light source with change over automatic or user selectable from 325 to 370nm Detector : Silicon Photodiode x 2 Wavelength Range : 190 to 1100nm Accuracy : 1.0nm Scan Speed : 3200nm/min or better Response Speed : Fast, Standard, Slow Photometric Range : -3 Abs to +3 Abs/0 to 600%T Accuracy : +0.002Abs at 0.5Abs or more Baseline Stability : 2hrs after power on Stray Light : 0.05% (220nm for NaI,340nm for NaN)2) Operating program : More than 15 Data storage : More than 5 sets at a time : Display Colour : LCD with backlit inbuilt Quartz cuvette : 2 pair Instrument : With Printer and serial interface Power Supply : 100-240V,50/60Hz, 200VA Installation : Free at site Warranty : 2 Years The Instruments should come with suitable data notebook with following specification 1.CPU :Intel core i5, core duo II 2.Operating system :Window 7 or more 3.Display :13 inch or larger 4. Pixel : min 1280x800 5.RAM :4 GB minimum 6. Disc storage :250 GB minimum 7. Processor speed :2.0 GHz or faster Optional :Rate for AMC for 5 years after 2 years warranty period The instrument is generic in nature The instrument should also work stand alone without computer Approx. Cost: 4.5 Lakhs Item No. 31 Item No. 32 Specifications of Bio safety Cabinet Type-III Bio- safety cabinet class-III Should have total exhaust system with ducting. Approximate interior Dimension (inches) of the cabinet : 25 H X 46 W X 24 D Adjustable base stand with at least height of 30 inches. All interiors and exterior should be of stainless steel. Should have HEPA filters for supply and exhaust for particle size 0.3 microns. Biological Safety cabinet should include the Zero Leak system, Drain Valve, Metal diffuser. Work Tray should be easily removable for cleaning purpose. Electronic airflow control system. Should have florescent light and UV lights. Vertical sliding view window w/8” working access and alarms. Electrical circuits should have circuit breakers for safety. Should include 1 electrical outlet and 2 service valves for air, gas or vacuum. Provision of armrest for ergonomic comfort and glove port for transfer of hazardous samples and to avoid cross contamination. Provision of Pass thru to provide easy transport of cultures and media without exposing to the environment. ECM motor to increase filter life, lower energy costs and reduce noise and vibration. Electrical requirement 230 V AV Air Handling Unit (AHU) to provide temperature uniformity for handling mycobacterium spp.
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