Estudio Experimental de larvas Hydrostatic spectral aquatic robot

Estudio Experimental de larvas Hydrostatic spectral aquatic robot
Estudio Experimental de larvas
Hydrostatic spectral aquatic robot laser rays, for larval mosquito control medical
importance in Public Health.
esign and build an aquatic robot to destroy the presence of larvae or pupae of
mosquitoes in water containers. It was built and a robot with recyclables built
with tubes pipe PVC, magnifier, light sensors and barrier, engine power 110 v,
resistors, LCR, charger 9 vy spotlights led, to destroy mosquito larvae was
designed in a container of water. As a result there is a zero prevalence Larval rate because the robot
detects larval presence sensors and lasers automatically activated with the suction effect and larvae
destruction their killing the inner cast off using filters of 10 microns and metal blades, the robot is
activated by five to ten minutes to wait automatically turns off the alarm again available as larvae.
Conclusion use water in water containers robot is not larval indices and pupae, which can be used as
anti larval control for transmitter combat Dengue, Zika, Chikungunya among others.
Keywords. Sensor robot larvae.
*Professor the department of microbiology at the University of El Salvador, El Salvador Central
Estudio Experimental de larvas
By January 2016 El Salvador has reported 3,302 cases of Zika with a rate of 51 x 1000
inhabitants and pregnant with 96 cases. In March 2016 confirmed cases of Zika in 31 countries, which
has caused international alarm. Diseases malaria, dengue and Chikungunya have not yet been
eradicated, Latin American countries have invested in the health care system millions of dollars to
combat the mosquito or mosquito, chemicals like Abbe are already reporting resistance to the larvae.
2014 and 2015 have been crucial to detecting and combating viral diseases, head to the Zika in which
have been associated microcephaly in newborns,
Malaria and dengue are two major diseases of medical importance, however the transmitter
Aedes aegypti transmits three potentially aggressive to human diseases, including dengue, Zika and
Chikungunya, when considered epidemiologically as Disease reporting statistical figures increasing by
the Ministry of Health. Defined as high risk in endemic areas. Similarly it has been reported today in
coastal regions and in urban areas with higher morbidity. Global efforts are sponsored or conducted by
WHO, PAHO and others to control and eradicate the vector, but has so far been impossible to achieve
promising results. We used various methods of control, in which the use of chemicals in plantations
and domiciliary areas highlighted, with the risk of causing poisoning in humans.
When malaria and dengue are two public health problems and governments, because millions
of dollars are invested in eradicating the vector and treat disease, derived from the national budget
allocated to health, today added Zika.
El Salvador is no exception, because its climate and population density makes favorable
transmission, predominantly in the dry and rainy season with temperatures between 220C to 300C in
coastal areas or tropical savannas and displacement heights less than 300 meters They make ideal
conditions conducive for your stay and spread.
80-90% of all cases in the country as reported by Malaria Ministry of Public Health and Social
Assistance (MOH), is located in endemic areas mainly on the Pacific coast. Reported rates with larger
numbers of cases have been in 2013 with more than 100,000 cases infected with malaria with an
approximate rate of 200 to 2.600 X 10.000 inhabitants.
Efforts to eradicate larvae using multiple resources by the Ministry of Health have been
systematic and advertising expenses is expensive. greater education and awareness with support for
Estudio Experimental de larvas
communities to collective and individual level is needed. When the Abbe intradomiciliar use as
prevention and sanitary control without the expected impact is made. Today fry used as fish hatcheries
in containers and sanitary control.
Hydrostatic design and build a robot that detects mosquito larvae and destroy larval index down.
Specific objectives:
1. Remove larvae through the hydrostatic robot and destroy them in a container of water.
2. Design an electronic circuit with automatic functionality.
3. Build a robot Hydrostatic with larvicide effect.
Methodological design .
The research is experimental application, with a research period of 15 months. The sample were
mosquito larvae stages I, II, III and IV. The independent variables studied are mosquito larvae
regardless of the species and the dependent hydrostatic robot preventing diseases like Dengue,
Chikungunya and Zika.
Results and discussion.
Lifecycle: In the experiment larvae of
several stages were collected, found in
its natural form in its life cycle first go
through egg lasting from 1 to 3 days
being in the water surface, can float
without difficulty Malaria, the other
species are grouped to float, then pass
larvae in 4 stages see Fig 1 that shed
their cuticle between each stage that
protects and thickens when they reach
stage IV, being the smallest initial and
immature I that others increase in size
at each stage, mosquito larvae need
water, have to inhale respiratory
Figura 1. LARVAS: I, II,
oxygen siphon except Malaria larvae
but not siphon is placed parallel to the
water surface for breathing. These larvae feed on small organisms and organic material to survive. On
Estudio Experimental de larvas
average, each larva is between 2 to 10 mm, has a long thin terminal and tube called a siphon that
serves to breathe in the water surface, last in this phase of 10 to 13 days, then pass pupa less dense
weight which easily fleet, which is the pre-adult stage mechanical pressure exiting the cuticle, at this
stage last 2 to 4 days. Then in adult phase are on average 3 to 6 weeks see Fig 2.3, males live less, the
female needs to mate in two days where they need to find water to lay eggs and especially the female
needs blood supply, looking male pollen, juices and other foods except blood. As epidemiological and
entomological surveillance is necessary to cut the cycle of life here in larval stage among others, and
will not transmit viral diseases in its chain of transmission.
Epidemiology: its main habitat is water, need oxygen in larval stage as a preliminary stage to reach
adult breathing. The larvae of malaria breeds in clean and contaminated water, dengue, Chickungunya,
Zika live in clean water. They live at an altitude less than 600 meters from sea level, are easily adapted
to temperate and warm climates.
Figure 2. Egg, larva, pupa and adult mosquito life cycle.
Estudio Experimental de larvas
A DAY IN THE LIFE OF A mosquito larvae.
The behavior of larvae for 8 hours was studied in a container of 2700 mts3, resulting larvae stages III
IV come to the surface to take in oxygen are between 5 to 10 seconds, any movement
defend submerging up background being between 10 to 15 seconds then go back
up to the surface. The time it takes to get from the surface to the bottom
depends on the depth of the container but arrive in 30 seconds and ascend
or descend a zigzag other queued between 20 to 30 sec. Stages I and
II larvae spend most of the time in the bottom of container until they
pass III and IV rise to the surface to take oxygen in the open diameter of
26x30 cm container with natural light, known in El Salvador as " water basin
". It is not known if they have photo-thermal heat and light receptors in the hair
antennas covering his body, but indicates that if have receptors, and have a pair of
antennae on his head. His behavior in the container looking light the biggest stadiums. They are
attracted to dark green in the background. It is a frenzy up and down continuously 24 hours a day in
the container until they leave adult.
Hydrostatic robot, by its nature does not move, which is submerged in the water bottom, no danger of
engine is a
because it has a voltage of 9 is 12 v equivalent to 6 batteries circuit sensor, the
water source increased pressure which is special for tanks without
danger of electroshock 110v, the robot has sensors that are
activated to interrupt a laser beam, which in turn activate the
alarm makes a focus 20-40 lighting turn the engine way
aspirator is activated for 5 minutes, then turn off automatically.
The laser beam is isolated from submerged water can be sealed for
24 hrs on or as needed, if something happens again a larva or more the
robot is automatically activated and the cycle begins again. It has
filters and metal cutting Crumbling turbines or destroys the larvae
are sucked, then pass the other end unmade, with a high mortality rate
in stages II to IV. When using a 10 micron filter mortality is zero prevalence. For proper operation
must first turn on the system for the sensors recognize their environment, then turn on the laser so that
the system shuts down and then left on the laser 24 hrs for I acted as a barrier and automatically
activate the robot to activated by the linear step photon laser light. It should not be handled by
Estudio Experimental de larvas
You must be integrated and have basic knowledge of medicine, optics, electronics, physics, electricity,
entomology and microbiology for the design and construction of the hydrostatic robot. The electrical
circuit has two terminals with positive charge and a negative, creating a closed circuit which activates
a load converting electric power of 12 v in a continuous electron flow translated into kinetic energy. In
the circuit there is a red LED that indicates that the sensor is activated and a red LED submerged as
well. an electronic circuit diagram, with a relay that is out of the water stimulates a source of UV light
to another sensor and this in turn automatically starts the engine powering the red LED submerged and
a focus of 20 wo 40w built warning that there larvae in the container. Hydrostatic robot consists of
electronic material that is the central circuit that processes the electrical information that is out of the
water, other material PVC is immersed with LED, filters and turbines, and other underwater sensors
and laser rays. The red LED lights submerged when the engine is on. It is constructed of PVC plastic
In the picture 1 the robot has several fotoresistencias as a signal source that captures light with photon
photodetectors several laser beams, which when interrupted the electrical signal, considered a barrier
activated. The circuit consists of 2 LCR, laser
pointer, red led a 2N2222 transistor, one resistor of
1 k 1 220 ohm resistor 1 resistor 2k, a relay, 1
connectors. 1 magnifying glass.
PHOTO 1. Diagram electronic circuit
The laser acts as a barrier, which is
submerged in the water directed to another
signal two fotoresistencias which are coated
with a transparent tube to avoid contact with water
and not damage the circuit, which receives light laser which faces a
30 cm.
According to the theory
CSF it is designed that their cells capture a mass based on its size, so that the larva is very small so
CSF diameter uptake covering with tape is decreased, so is directly proportional to light intensity
increases its resistance to high light otherwise least resistance. The laser is monochromatic photon
emission in one direction, producing an electromagnetic source, capturing any movement when
Estudio Experimental de larvas
passing larvae interrupting the electrical signal, its wavelength is 630 nm from
760 to. Your color is red. This allows the circuit is turned off and turned on
again when the laser beam is stopped and the cycle begins again. Tests
were made to place more than 5 LCR 5 laser rays and the same results, in
our case we use two with decreasing diameter of laser light gathering.
test was done on a small scale water out how the laser to pass the
4 Length of laser wave ray.
Estudio Experimental de larvas
PHOTO 3. LASERS AND ROBOT MOTOR submerged in water.
In Figure 4 and Photo 2.3. It is observed the robot immersed in water in the presence of laser
beams, the larvae when approaching the laser cutting laser light meaning that triggers the activation of
the motor to be suctioned and be destroyed having a perimeter scope shaft suction 360 . the red LED at
the top indicating that the engine is running and the LED lights of the electronic circuit. Clarifies that
only the laser can be turned on 24 hours, also can be constructed with laser diode of a burner with
potentiometer to avoid burning the larva, its operation activates the robot when the signal is cut it
activates the whole system including the electronic circuit which in turn stimulates the sensor to the
engine and red lED submerged focus is scheduled for five to ten minutes or thirty minutes then turns
off automatically except the laser is activated. If there is much movement of larvae is not quenched. In
summary entire circuit robot has two primary sensors, one motion sensor is converted to light sensor
or light sensor that simply is not submerged and the other is the sensor barrier CSF is submerged. The
robot is placed at the center of the water container because the larvae need light and oxygen.
The robot generally consists of three parts: the first is the electric circuit containing the LCR
submerged, the second light sensor is not submerged, which in turn activates the motor submerged
once and a light source not submerged alerting larvae and a red lED indicating the motor is activated
and the third signal laser beams is directed to submerged CSF.
Estudio Experimental de larvas
Efect a concentrationes de 5,10 y 15
y = 0.7667x + 2.9
R² = 0.9379
Vivas o muertas
Linear ()
y = 0.0909x + 0.8182
R² = 1
Linear ()
FIGURE 1. Effect at concentrations of 5.10 and 15 larvae when using the robot.
In Figure 1. In the container was found that the robot can destroy or dispose of larvae detection in 99100% accurate enough larvae, since through the light beam, which is optional the same circuit LCRs
have several and several more pointers to more is the probability of detection.
Efect larvicide
Estudio Experimental de larvas
FIGURE 2. Effect larvicide to greater numbers of larvae.
In Figure 2 it was found that the more larvae the robot has a larvicide, capable of automatically
activated when cutting the beam of laser light, indicating that there is presence, so the container can be
free of larvae effect. Always seek the larvae surface to take oxygen in the light aperture diameter of
the container and immersed when in danger. It also detects pre-pupae are adults.
The water in water containers robot when activated is not larval and pupal indices, which can
be used as the transmitter control antilarval combat Dengue, Zika, and Chikungunya among others.
Bibliographic references.
1. Pardos G, (2005). Algoritmos de geometría diferencial para la locomoción y
navegación bípedas de robots humanoides aplicación al robot RHO. Madrid.
2. Murray PR. Rosenthal. Microbiología Médica. 7a edic Ed. Elsevier. Barcelona
España. 2013.
3. Joklik, W. Microbiología de Zinsser, 20 edic. Edit Panamericana, Argentina. 1996.}
4. Sherris, J. Microbiología médica. 4ª edic. Edit. Mac Graw Hill. Barcelona. 2004.
5. Internet.
6. Internet.
7. Internet.
8. Ministerio de Salud Pública. Reporte Epidemiológico. 2013,2015-2016
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