Axiocam 503
User Guide
Axiocam 503 color/mono
Microscope Camera
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
[email protected]
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen
Germany
Effective from: October 2014
© Jena 2014 by Carl Zeiss Microscopy GmbH - all rights reserved
This document or any part of it must not be translated, reproduced, or transmitted in any form or by any means, electronic or mechanical, including
photocopying, recording, or by any information or retrieval system. Violations will be prosecuted.
The use of general descriptive names, registered names, trademarks, etc. in this document does not imply, even in the absence of a specific
statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Software programs will
fully remain the property of ZEISS. No program, documentation, or subsequent upgrade thereof may be disclosed to any third party, unless prior
written consent of ZEISS has been procured to do so, nor may be copied or otherwise duplicated, even for the customer's internal needs apart from
a single back-up copy for safety purposes.
ZEISS reserves the right to make modifications to this document without notice.
2
Content
1
About this guide
5
1.1 Introduction
5
1.2 Safety notes conventions
6
1.3 Text formats and conventions
6
2
Safety
8
2.1 Safety Notes
8
2.2 Limitation of liability
9
2.3 Warranty
9
3
Technical Data
10
3.1 Axiocam 503 color/mono
3.1.1 Binning Mode and Frame Rate
3.1.2 Live Frame Rates
3.1.3 Spectral Sensitivity
10
13
13
14
4
Shipment
15
5
Connecting the camera
16
5.1 Camera Overview
16
5.2 Building in the interface card
17
5.3 Mounting the camera on the microsope
18
5.4 Connecting the camera to the PC
19
5.5 Connecting the trigger cable
19
5.6 Function indicator
21
6
22
Installing Software and drivers
6.1 Installing ZEN software and drivers
22
7
23
Acquiring images
7.1 Introduction
23
7.2 Acquiring the first image in ZEN
23
7.3 Fluorescence images
24
8
25
Trouble-shooting
8.1 Software
User Guide Axiocam 503 color/mono | V 1.1 en
25
3
Content
8.1.1
8.1.2
8.1.3
4
The camera does not appear in the menu of selectable cameras
You don't see a camera image on your screen
The color of my image does not correspond to the impression
through the ocular
25
25
25
8.2 Hardware
8.2.1 Vibrations
25
25
9
26
Maintenance
9.1 Optical System
26
9.2 Cleaning the infrared filter or protective glass
26
10
27
Disposal and Recycling
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1 About this guide | 1.1 Introduction
1 About this guide
1.1 Introduction
Welcome Welcome to the Axiocam 503 user documentation.
The camera is a professional digital camera for universal light microscopy with a
high resolution sensor and a USB 3.0 interface. The camera can be ordered as color
camera (Axiocam 503 color) or monochrome camera (Axiocam 503 mono). As
setting up the two camera models is the same, this guide stands for both camera
models.
The differences in the specs can be found in the chapter Technical Data [} 10]. To
set up the camera correctly, follow the instructions in this guide step by step.
Content
Chapter
Content
About this manual
Introduction and overview about this manual.
Safety
Important information on a safe handling with the
Axiocam 503. Read this chapter, before
unpacking the camera and putting it into
operation.
Shipment
The contents of delivery and optional attachments
will be described here.
Technical data
Here you will find all technical data to your
camera.
Connecting the camera
In this chapter you will find detailed instructions
on connecting and using the camera.
Installing software and
drivers
Here you will learn how to install the software
(e.g. ZEN 2012 (blue edition)) and camera drivers.
Acquiring images
This chapter provides instructions for basic image
acquisition using ZEN 2012 (blue edition). You can
find more detailed descriptions of camera
functions in the online help of the ZEN 2012 (blue
edition) software.
Trouble-shooting
In this chapter we have listed some solutions to
various problems. If you can not solve your
problem, contact the ZEISS support.
Maintenance
This chapter describes some measures for the
maintenance and care of your camera. For greater
damage always contact the ZEISS support.
User Guide Axiocam 503 color/mono | V 1.1 en
5
1 About this guide | 1.2 Safety notes conventions
Chapter
Content
Disposal and Recycling
Important instructions for disposal and recycling.
1.2 Safety notes conventions
The safety notes in this document follow a system of risk levels, that are defined as
follows:
CAUTION
Risk of personal injury
CAUTION indicates a potentially hazardous situation which, if not avoided, may
result in minor or moderate personal injury.
NOTICE
Risk of property damage
NOTICE indicates a property damage message. In addition, NOTICE is used for
data loss or corrupt data as well.
Info
An info indicates useful additional information. Infos help you to make your daily
work easier, but they are all optional. There is no risk for personal injury or
property damage involved.
1.3 Text formats and conventions
Bold texts
Bold is used for texts within the software like names of GUI elements (e.g. buttons,
sections, tools, menus), key commands (e.g. Crtl + C), buttons on a device, product
names, etc.
Font type "Courier"
Font type "Couriere" is used for programming code. E.g.
macro code as well as for anything that you would type
literally when programming, including keywords, data
types, constants, method names, variables, class names,
and interface names.
6
User Guide Axiocam 503 color/mono | V 1.1 en
1 About this guide | 1.3 Text formats and conventions
Shortcuts and key commands
Shortcuts do appear like Crtl+C , that means you must press Crtl-Key and C-Key
together.
Procedures
Following formats are used for procedures (instructive sequences):
Prerequisites ¢ Stands for a condition which must be fulfilled before starting with the action.
Procedure 1
Stands for a single step the user is asked to perform.
Web-Links
Web links do appear in blue text color. To open the linked website, simply right
click on the link. Just in case you are not connected to the internet, make sure you
have a internet connection established before opening the web link.
User Guide Axiocam 503 color/mono | V 1.1 en
7
2 Safety | 2.1 Safety Notes
2 Safety
2.1 Safety Notes
The Axiocam 503 has been manufactured and tested by ZEISS according to the
regulations specified in CE and has left the manufacturer’s premises in perfect
working order. In order to ensure that this condition is maintained and to avoid any
risks when operating the system, the user must comply with any notes and
warnings contained in this manual. The manufacturer shall be exempt from
statutory liability for accidents should the operator fail to observe the safety
regulations.
CAUTION
Personal Injury
To avoid personal injury, read and adhere the safety notes below.
u To avoid the risk of fire or explosion, do not use the camera near
inflammable liquids or gases.
u Setup, expansions, re-adjustments, alterations, and repairs must be carried
out only by persons who have been authorized by ZEISS.
u Do not allow any cables, particularly power cords, to trail across the floor,
where they can be snagged by people walking past.
u Protect the cables from excessive heat (e.g. halogen lamps, microscope
fluorescence illumination).
8
User Guide Axiocam 503 color/mono | V 1.1 en
2 Safety | 2.2 Limitation of liability
NOTICE
To avoid equipment damage, data loss or corrupted data, read and adhere the
safety notes below.
u Protect the camera against mechanical impact. External damage may affect
the operation of inner components.
u Keep chemicals and fluids away from the camera.
u Make sure there is sufficient ventilation of the camera head. Avoid direct
exposure to sunlight and locations near heat sources (radiators, stoves).
Overheating can cause noisy images.
u Use the camera in a clean and dry location.
u Attach all connectors firmly and securely.
u Use only the accessories supplied by ZEISS, when applicable.
u Use only normal microscope cleaning material to clean the camera housing.
u Contact your local ZEISS service organization, if a repair is necessary.
u Save all your data, such as images, measurement data, archives, reports,
forms and documents, at regular intervals on an external storage medium.
Otherwise it cannot be avoided that access to this data may be lost as a
result of operational errors or hardware defects. ZEISS accepts no liability for
consequential damage resulting from insufficient data protection.
2.2 Limitation of liability
No warranty shall be assumed by ZEISS during the warranty period if the equipment
is operated without observing the safety regulations. In any such case, ZEISS shall
be exempt from statutory liability for accidents resulting from such operation.
2.3 Warranty
ZEISS shall be exempt from any warranty obligations should the user fail to observe
the safety regulations. ZEISS only guarantees the safety, reliability, and performance
of the system if the safety notes are closely observed.
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3 Technical Data | 3.1 Axiocam 503 color/mono
3 Technical Data
3.1 Axiocam 503 color/mono
Feature
Value
Sensor Model
Sony ICX 674, EXviewHAD CCD II ™
Progressive Scan, Quad-Port Readout
architecture, pre-selected sensors for highest
image quality
Sensor Pixel Count
2,83 Megapixel: 1936 (H) × 1460 (V)
Pixel Size
4.54 µm x 4.54 µm
Sensor Size
Image Diagonal 11 mm , equivalent to 2/3”"
Sensor Format
Spectral Sensitivity
Axiocam 503 color:
app. 400 nm-720 nm, coated BG 40 IR Cut
Filter,
RGB Bayer color filter mask
Axiocam 503 mono:
app. 400 nm-1000 nm, coated BK 7
protective glass
10
Max Full Well Capacity
(typical)
15.000 e- per pixel
Signal Amplification
Adjustable analog amplification 1x, 2x, 3x
Digitization
14 Bit / Pixel
Read Out Speed
Switchable readout clock speed 39 MHz / 13
Mhz
Readout Noise
(typical)
6.5 e- at 39 Mhz
6.0 e- at 13 Mhz
Dynamic Range (typical)
1:2500 (68dB)
Dark Current (typical)
< 0,06 e-/p/s at 18 °C sensor temperature
Cooling
Regulated Peltier-Cooling,
(power supplied through USB 3.0 and USB 2.0
ports) Delta-T 20° C, sensor temperature 18°C
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3 Technical Data | 3.1 Axiocam 503 color/mono
Feature
Value
Dark Current Compensation
Digital Dark Current Compensation for
optimum low light performance at long
exposure times,
Automatic Hot Pixel Correction
Exposure Time Range
250 µs to 60 s
Color Interpolation Modes
Axiocam 503 color:
“High Speed ”: optimum speed color
interpolation
“High Quality ”: optimum quality color
interpolation
Special Preset Modes
Eight pre-loadable sets of imaging parameters
for speed-optimized multi-modal image
acquisition,
Overlapping exposure and readout for fast
time lapse imaging
Switchable Sensor Output
Amplifier
Single Port Readout mode for long exposure
times for maximum signal quality, Dual Port
and Quad Port Readout Mode for improved
readout speed at full resolution,
Automatic port activation mode or full manual
mode
Region of Interest (ROI)
User defined imaging sub area for
improvement of readout speed and reduction
of amount of data
Hardware Trigger
Galvanic isolated I/O signals,
three output signals: exposure time, readout
time, trigger ready,
i.e. for controlling external mechanical
shutters,
one trigger input for exposure control,
5V auxiliary voltage, GND
Status LED
Top LED: Camera Status (acquisition, power,
cooling, speed)
Back LED: Trigger Status
Interface
USB3.0 SuperSpeed (5 Gbit/s), bandwidth
max. 240 Mbytes/s
USB2.0 optional, with lower speed
Optical Interface
C-Mount (17.5 mm)
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3 Technical Data | 3.1 Axiocam 503 color/mono
Feature
Value
Max. File Size per image
Axiocam 503 color:
app. 17 MB per image with 1936 x 1460
Pixels
at 3 x 14 Bit/Pixel
Axiocam 503 mono:
App. 5,6 MB per image with 1936 x 1460
Pixels
at 14 Bit/Pixel
Operating Systems
MicrosoftÒ Windows 7 Enterprise and higher
Size / Weight
10.8 cm x 7.8 cm x 4.3 cm / 500 g
Housing
Blue anodized aluminum, ¼“ thread for
camera equipment,
Zero Vibration by convection- cooling,
optimized cooling finns,
Teflon coated C-mount thread,
Certificates
CE
Power Supply
Max. 7W power consumption,
power by USB2.0 and USB3.0-Bus from PC
For maximum performance connection to USB
3.0 and USB 2.0 required, dual connection
cabling provided with camera
Ambient Conditions
(Operation)
+5° ... +35° Celsius
max. 80% relative humidity, non-condensing,
free air circulation required,
Ambient Conditions (Storage)
-15° ... +60° Celsius
90% relative humidity at +40° Celsius,
80% relative at +20° Celsius, non-condensing
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3 Technical Data | 3.1 Axiocam 503 color/mono
3.1.1 Binning Mode and Frame Rate
Binning
HxV
Mode
FPS @ 1ms
1x1
1936 x 1460
Color/Mono
38
2x2
968 x 728
Mono
61
3x3
640 x 484
Color/Mono
75
4x4
480 x 364
Mono
87
5x5
384 x 292
Color/Mono
93
ROI
2752 x 1080
Color/Mono
45
ROI
2752 x 512
Color/Mono
69
3.1.2 Live Frame Rates
Axiocam 503 color Max. Ratings at optimum settings; Hardware and color enhancement off:
Max. Frame Rate
Binning Factor /
Mode
Pixel Count
38 frames/s
1 / slow
1936 x 1460
75 frames/s
3 / medium
640 x 484
93 frames/s
5 / fast
384 x 292
Axiocam 503 mono Max. Ratings at optimum settings
Max. Frame Rate
Binning Factor /
Mode
Pixel Count
38 Bilder/s
1 / slow
1936 x 1460
61 Bilder/s
2 / medium
968 x 728
76 Bilder/s
3 / fast
640 x 484
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3 Technical Data | 3.1 Axiocam 503 color/mono
Info
Computer hardware, operating system and software may decrease the frame
rates. Selecting a part of the sensor area or applying binning can increase the
frame rate. All specifications are subject to change without notice.
3.1.3 Spectral Sensitivity
Axiocam 503 color
Illustration 1: Spectral Sensitivity Axiocam 503 color
Axiocam 503 mono
Illustration 2: Spectral Sensitivity Axiocam 503 mono
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User Guide Axiocam 503 color/mono | V 1.1 en
4 Shipment
4 Shipment
Content
Order Number
1 x Axiocam 503 color camera or
1 x Axiocam 503 mono camera
426558-0000-000
426559-0000-000
1 x USB cable (2 in one) for power supply (USB 2.0 =
black cable) and data transfer (USB 3.0 = blue cable)
1 x PCI express interface card ( 4 x USB 3.0)
1 x 15 Pin SATA power connector cable
1 x DVD with device driver for ZEN as well as this
guide as a PDF file.
Accessory (optional)
Illustration 3: Axiocam trigger cable
Content
Order Number
Trigger cable
426557-0001-000
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15
5 Connecting the camera | 5.1 Camera Overview
5 Connecting the camera
5.1 Camera Overview
Camera connections
Illustration 4: Camera back
16
Number
Description
1
Trigger connection
2
USB 2.0 connection for power supply and cooling
3
USB 3.0 connection for image / controller data and power
supply
4
¼ “ photo thread (tripod connection)
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5 Connecting the camera | 5.2 Building in the interface card
5.2 Building in the interface card
This chapter describes how to build in the PCI express interface card (4 x USB 3.0)
into your PC.
NOTICE
Static Electricity
Static electricity can damage electronic components. To protect electronic
components against static electricity, do not touch them until you have
grounded yourself to the casing of the device. Never touch the contacts of the
electronic components. We also recommend that you work only on an antistatic
mat.
Prerequisites ¢ You have read the documentation of the interface card and of your computer
before you build in the interface card.
Procedure 1
Switch off your PC and all connected peripherals.
2
Disconnect the PC and the peripherals from the mains and open the computer
case.
3
Examine your PC’s power supply to see if it has Molex type connectors or SATA
type connectors. Choose the appropriate power connector cable for the
following steps. If your PC’s power supply has both connector types, choose
only one cable.
4
Connect the appropriate power connector cable to one of the open
connectors from your computer’s power supply.
5
Connect the other end of the power cable to the PCIe interface card.
6
Insert the PCIe interface card into the appropriate slot on your computer.
7
Close the computer case and reconnect the peripherals.
You can now mount the camera on the microscope and connect it to the PC via
the USB 3.0 interface, see Connecting the camera with PC.
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5 Connecting the camera | 5.3 Mounting the camera on the microsope
5.3 Mounting the camera on the microsope
To mount the camera onto your microscope’s TV port, use a C-mount adapter. The
adapter is not included in the shipment. You will find some suitbale examples for
adapters in the list below:
Port
Name
Order Number
44
Video Adapter 44 C 2/3" 1,0x
452995-0000-000
60
Camera Adapter 60 C 1" 1,0x
456105-9901-000
60
Video Adapter 60 C 2/3" 0,63x
000000-1069-414
60N
Camera Adapter 60N-C 1" 1,0x
426114-0000-000
60N
Camera Adapter 60N-C 2/3" 0,63x
426113-0000-000
NOTICE
Loss of warranty
The Axiocam 503 color is delivered with an integrated IR filter (infrared cut
filter). The Axiocam 503 mono is delivered with an integrated BK7 protective
glas. This will protect the camera against dust and reduces optical interferences.
u The IR filter is situated approx. 5 mm behind the outer edge of the C-mount
opening in the camera.
u Due to this pre-mounted IR barrier filter, C-mount adapters that screw more
than 5 mm into the thread cannot be screwed into the camera.
u Do not remove the filter or the protective glas otherwise the warranty will
be lost.
Procedure 1
18
Remove the dust cap from the camera’s C-mount port.
2
Screw the adapter in as far as it will go.
3
Mount the camera onto the microscope’s TV port. Ensure that no dust enters
the opening of the camera or the microscope’s TV port.
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5 Connecting the camera | 5.4 Connecting the camera to the PC
5.4 Connecting the camera to the PC
Procedure 1
Lay the USB cable (2 in 1) carefully between the camera and the PC.
2
Connect the USB cables to the USB 3.0 interface card of the PC.
3
Connect the USB cables with the camera.
4
If you switch on the PC, the camera will be also switched on.
Power is supplied via the USB 3.0 cable. The USB 2.0 cable supplies the peletier
cooling. By switching off the PC, the camera will be switched of as well. If the
camera is assembled correctly, the status LED will light red. If you start the software
the LED will turn blue. You`ll find a detailed description of the LED status light in the
chapter Function indicator [} 21]
5.5 Connecting the trigger cable
In this chapter you will learn how to connect the trigger cable (optional) to the
camera and the SVB (signal distribution box).
Illustration 5: Trigger cable
Number
Description
1
Trigger (Trigger-In)
2
GPO 0 (Trigger Out, for exposure time incl. predelay,
coresponding to shutter control
3
GPO 1 (Trigger Ready, for Readout Time data)
4
GPO 2
5
GPOPWR
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19
5 Connecting the camera | 5.5 Connecting the trigger cable
Procedure 1
2
Number
Description
6
AUXPWR
7
Commercial Micro-D
Plug the connector Commercial Micro D (7) in the trigger connector on the
back of the camera, see Camera Overview [} 16].
Plug the connector Trigger (1) in the socket Trigger Out of the SVB (Signal
distribution box).
Illustration 6: Signal distribution box (SVB) backside
20
3
Plug the connector GPO 0 (2) in the socket Trigger In of the SVB (Signal
distribution box).
4
Connect the connectors GPOPWR (5) and AUXPWR (6) of the trigger cable
together.
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5 Connecting the camera | 5.6 Function indicator
5.6 Function indicator
Signal color
Description
Blue
USB 3.0 connection (Camera) connected with USB 3.0
connection (PC)
Power supply (USB 2.0 cable) attached
Recommended configuration for best camera performance
Green
USB 3.0 connection (Camera) connected with USB 2.0
connection (PC)
Power supply (USB 2.0 cable) attached
Violet
USB 3.0 connection (Camera) connected with USB 3.0
connection (PC)
Power supply (USB 2.0 cable) not attached
Yellow
USB 3.0 connection (Camera) connected with USB 2.0
connection (PC)
Power supply (USB 2.0 cable) not attached
Red
Power supply attached, no driver loaded, camera not yet
initialized
No light
No power supply or software shut down (camera in power
down mode)
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6 Installing Software and drivers | 6.1 Installing ZEN software and drivers
6 Installing Software and drivers
6.1 Installing ZEN software and drivers
Prerequisites ¢ To acquire images with the Axiocam 503 on a PC, you must install ZEN
software. You will find the software installation on the DVD delivered. The
camera drivers will be installed automatically during the installation of the
software.
¢ Before starting the installation of the software and drivers the camera must be
connected to the PC, see chapter Connecting the camera to a PC.
Procedure 1
Install the software according to the installation guide delivered with the
software.
2
During the installation you will be asked to install the camera drivers. Follow
the procedure by confirming the upcoming messages.
3
Restart your PC after the installation.
4
You can check in the device manager if the driver installation was successfully.
You have installed the software and the camera drivers. You can now start with
acquiring images with your camera.
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7 Acquiring images | 7.1 Introduction
7 Acquiring images
7.1 Introduction
The Axiocam 503 color is a professional digital camera with a high-resolution 5
megapixels CCD color sensor for universal application in the field of light
microscopy. The color camera is suitable for all situations where stained or naturally
colored samples need to be acquired quickly and with very high camera resolution.
As it has a number of selectable acquisition modes, the camera can be adapted
flexibly in terms of acquisition speed and quality to suit a variety of acquisition
conditions. The 12-bit digitization of the sensor signals ensures an optimum
combination of spatial definition and outstanding resolution of intensity levels. The
camera’s very low level of background noise (supported by active Peltier cooling of
the sensor), and possible exposure times ranging from 1 ms up to 60 sec, make it
suitable for an extremely wide range of applications in the field of microscopy.
7.2 Acquiring the first image in ZEN
This topic guides you through acquiring your first image with ZEN 2012 (blue
edition) software.
Prerequisites ¢ You have connected and configured a microscope camera to your system.
¢ You have started the software.
¢ You have configured the microscope components (e.g. objective, camera
adapter) und you are using the automatic or manual scaling.
¢ You are on the Camera (ZEN lite only) or Locate tab.
¢ You see your microscope camera available in the Active Camera section. If
not, select the camera from the list.
Procedure 1
Position your sample on the microscope and adjust the microscope to see a
focused image through the eyepieces.
2
Make sure your microscope is set to divert the image to the camera
(e.g. Adjust the tube slider of the microscope to 50% camera and 50%
eyepieces).
3
Click on the Live button.
The Live Mode will be activated. You will recognize the Live Mode by the
green signal and by the hatched tab in the Document Bar. In the Center
Screen Area, you will see the camera`s live image. By default the live image
shows a cross hair, to help in navigating around the sample. In the chapter
Optimize Live Image settings, you will learn how to optimize the live image
display.
4
Click on the Set Exposure button.
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7 Acquiring images | 7.3 Fluorescence images
The exposure time will be automatically determined and set.
Info
If you do not see a focused image, refocus the sample on the microscope. You
can activate the focus bar as an additional aid. Open the context menu in the
Center Screen Area via a right mouse click. Select the entry Focus Bar. The
focus bar will be shown.
5
Click on the Snap button.
You have successfully acquired your first image with ZEN blue. Save the image in
the file system via the menu File | Save as.
7.3 Fluorescence images
Axiocam 503 mono The Axiocam 503 mono differs from the Axiocam 503 color in an enhanced
spectral sensitivity of the CCD sensor. Because of the sensor having no color filter
mask applied and no IR filter is needed unfiltered light will reach the sensors pixels.
That's why the camera is best suited for fluorescence imaging.
Axiocam 503 color Due to its high sensitivity and the long exposure times that are possible, the
Axiocam 503 color can also be used for fluorescence images in the field of
microscopy. However, please bear in mind the following points: The color sensor
uses a so-called Bayer color filter mask on the pixels in order to produce color.
Consequently, pixels with a red filter are sensitive only to red spectra, pixels with a
blue filter only to blue spectra, and pixels with a green filter only to green spectra.
These filters are distributed in such a way that half of all the pixels are covered with
green filters. Red and blue filters each cover a quarter of the pixels. In the case of
fluorescence, however, only very narrow-band light is emitted. This is received only
by the color pixels that are sensitive to it. Consequently, the resolution of images of
this type is reduced in comparison with white light.
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8 Trouble-shooting | 8.1 Software
8 Trouble-shooting
8.1 Software
8.1.1 The camera does not appear in the menu of selectable
cameras
¢ Make sure that you have connected the camera and installed the software and
drivers in accordance to the instructions in this manual.
¢ Make sure that you installed the software and drivers with administrative
rights. Install the driver with administrative rights again if necessary.
8.1.2 You don't see a camera image on your screen
¢ Check the light path setting of the microscope.
¢ Is the status LED on the camera on? If not, check the cable connections
between camera and computer.
¢ Execute a reset of the camera.
¢ Execute an automatic exposure measurement.
¢ Check the display adjustments for the live image.
¢ Check the aperture diaphragm of the microscope.
¢ Check the position of the beam splitter between the ocular and the TV port.
8.1.3 The color of my image does not correspond to the
impression through the ocular
¢ Check white balance and, if necessary, repeat white balance.
¢ Check the monitor’s color temperature setting. If necessary, reduce this to the
lowest value that can be set (usually 5200 K).
8.2 Hardware
8.2.1 Vibrations
Sudden jolt, vibration or moving objects during the acquisition can detract the
image quality. The intensity of light during exposure time can change as well.
¢ To avoid jolt use damping systems on your microscope.
¢ Repeat the shot and take care to ensure the conditions remain calm.
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25
9 Maintenance | 9.1 Optical System
9 Maintenance
9.1 Optical System
The internal optical components of the camera should always be protected. If no
lens, or TV adapter with optics, is screwed into the camera's C-Mount thread, the
camera's sensor and IR-filter must be protected by screwing the protective cap
onto the camera's C-Mount thread.
9.2 Cleaning the infrared filter or protective glass
Contamination of the IR filter or the protective glass has an adverse effect on the
quality of the resulting image (dark points, cloudy structures in the image). If there
is dry dust on the front side of the infrared filter or protective glass, you can remove
it with a soft brush or with cotton (wool). Use cleaning fluid for optics/lenses only
to clean the IR filter. Do not use tap water to clean the IR filter.
26
User Guide Axiocam 503 color/mono | V 1.1 en
10 Disposal and Recycling
10 Disposal and Recycling
This product has been developed, tested and manufactured in accordance with the
applicable environmental provisions and directives of the European Union:
¢ The product and its accessories comply with EU directives 2002/95/EC (RoHS)
and 2002/96/EC (WEEE), insofar as these apply to this product.
¢ ZEISS has implemented a take-back and recycling process that ensures that
proper recycling is carried out in accordance with the aforementioned EU
directives.
¢ Please contact your ZEISS sales/service organization for details relating to
disposal and recycling.
¢ This product must not be disposed of with domestic waste or using municipal
waste disposal services. In the event of resale, the seller must inform the buyer
of the need to dispose of the product appropriately.
User Guide Axiocam 503 color/mono | V 1.1 en
27
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
[email protected]
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen
Germany
ZEISS reserves the right to make modifications to this document without notice.
© Jena 2014 by Carl Zeiss Microscopy GmbH - all rights reserved
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