LIFE SCIENCE APPLICATIONS Image-Pro Premier ® Image Analysis Starts Here Image Analysis Starts with Image-Pro® Incorporating more than 25 years of user input, Image-Pro Premier provides intuitive tools that make it easy to capture, process, measure, analyze, and share your images and valuable data. Rely on our worldwide network of Image-Pro partners, superb training tools, and technical support team to help you get started and keep your research moving forward. Try Image-Pro Premier today and discover what makes Image-Pro from Media Cybernetics® the leading image analysis software. Designed for researchers who need automated measurement and analysis tools: • Anatomy • Animal Behavior • Brightfield Microscopy • Cell Biology • Dendrochronology • Developmental Biology • Entomology • Fluorescence Imaging • Food Science • Marine Biology • Otolith and Fish Aging • Pathology Easily Capture Single-Frame Images & Time-Lapse Experiments Successful analysis begins with high-quality images. Image-Pro Premier supports an evergrowing list of scientific cameras to ensure a superior capture experience. Live Tiling – No Automation Needed Stitch together multiple fields of view as you manually move around your specimen. Combine with Live EDF to create a focused, tiled image. Live Measurements Measure distances, angles, object areas, and more while previewing live images. No need to save your image. Fast Acquisition & Streaming to Disk Acquire rapid events or experiments that last for longer periods of time. Image-Pro Premier is not only able to utilize the memory (RAM) available on your computer, it can also stream multi-gigabyte movies directly to your hard drive. www.mediacy.com | [email protected] Live Extended Depth of Field – No Automation Needed Capture beautifully focused images, regardless of depth. Process Analyze Process && Analyze Implement sophisticated image image processing andand enhancement Implement sophisticated processing enhancement functions with two clicks or less. Image-Pro Premier makes it functions with two clicks or less. Image-Pro Premier makes it easy easy to analyze and reveal important details within your images. to analyze and reveal important details within your images. Correct for Noise & Enhance Detail with Filters Auto-Alignment & Extended Depth of Focus Correct for microscope shift with post-acquisition auto-alignment tools. Extended Depth of Focus (EDF) creates a focused image from a series of images captured at different z planes. Overlay & Compare Images Overlay images on top of one another to see changes over time. Use with previously acquired or live images. Extract & Merge Color Channels Remove background noise and reveal hidden details with an extensive set of image processing filters. Preview filters on your active image for instant results. Navigate & Edit Movies in Gallery View Navigate through time-lapse and image sequence files with the Gallery View tools. Easily combine and separate images into RGB, HSI, and HSL color channels. Post-Acquisition Tiling Tile together a series of images with post-acquisition tiling tools and view a full representation of your research sample. Batch-Process Multiple Images FREE Video Tutorials Visualize Images in 3D Explore the depth of your images by visualizing in three dimensions. Easily create animations to share. Apply your image processing steps to all opened images or entire folders of images utilizing Image-Pro Premier’s easy-to-use Batch Processing tools. www.mediacy.com | [email protected] Measure & Quantify Extract quantifiable data from your images with a variety of measurement tools. Easily measure distances, regions, angles, and more. Free Online Tutorials and Webinars Classify Measurements Use different colors and names to classify and organize your measurements. Export Measurements to Excel With the click of a button, your measurement and data graphs can be exported to Microsoft Excel for further analysis. Save Measurement Overlays Measurements are saved in your image’s meta data and are recalled the next time you open the image in Image-Pro Premier. Measure Intensity over Time Track and graph the changing of intensity parameters over time within an area of interest. www.mediacy.com | [email protected] Analyze Ringed Structures & Detect Edges The Caliper/Line Profile tool automatically detects edges along a defined line. Measure distances between these changing intensity levels to analyze aging. Classify edges to aid in analysis and reporting. Track Moving Objects Manually or automatically follow cells or other organisms as they move through time and space. You can even track challenging objects that fade out of view and move out of the image workspace. Count & Classify Use one-click tools to automatically segment, count, measure, and characterize objects. Free Online Tutorials and Webinars Measure Distances between Objects Measure one-to-one and one-to-many distances between objects. Analyze the Spatial Distribution of Objects Use the Voronoi Map feature to analyze and display the spatial distribution of objects. Segment Difficult Objects with Smart Segmentation Use Smart Segmentation to identify and segment challenging images with faintly colored objects, textured objects, and uneven backgrounds. Sort Counted Objects Create a new image displaying all counted objects arranged by size. Automatically Select & Classify Objects Locate and segment objects based on their color or intensity levels. Create new classes to further characterize and streamline data collection and reporting. Measure Objects within Objects Analyze parent/child relationships with tools that allow you to automatically measure and group objects within objects. www.mediacy.com | [email protected] Report & Share Now that your analysis is complete, you need to be able to share your findings. With Image-Pro Premier, it’s easy to prepare your imaging research to share with others. Automate Your Imaging Processes Image-Pro Premier’s automation tools not only save time by eliminating repetitive steps, but more importantly, they minimize the chance of errors or inconsistencies. Annotate & Copy Images Add descriptive annotations to single images or within movie frames. Choose to save annotations as independent overlays or as part of the image data. Create zoomed-in copies of your images and measurement overlays to use in print or web publications. Record Your Work Automate your imaging tasks with simple macro recording tools. Play back macros or combine multiple macros to ensure accurate results every time. Graphical or Code-Based Macro & App Editing Macros and apps are easy to edit with Image-Pro Premier’s graphical dragand-drop editing interface. You can also use the code-based editing tools to test, edit, and debug your scripts. Create Document Your Steps Using the Audit Trail Create Reports in PDF, Microsoft® Word®, PowerPoint® & Excel® Sharing your images and data is simple with easy-to-use report templates. Export images, graphs, and measurements to Excel. Send single images or groups of images directly to PowerPoint slides. FREE Video Tutorials www.mediacy.com | [email protected] Save and document your image processes with the Audit Trail. Document entire sessions as well as steps performed on specific images. Export your audit trail as an XML file or to Microsoft Excel. Download Share Create, Download & Share Custom Apps Image-Pro Premier’s custom apps allow you to easily design step-by-step workflows that walk you through your image analysis processes. Download apps from Media Cybernetics’ Solutions Zone website or choose to develop and package your own apps to share with colleagues. Image-Pro Premier Features Capture Measure & Quantify Automate Imaging Tasks • Single-frame & movie acquisition • Advanced line profile analysis • Automate tasks with recorded macros • Streaming to disk Manual measurements • • • Instant acquisition Measure live images • • Macro programming for more advanced users Crosshair grid overlay Snap-to-edge measurements • Macro packaging for easy sharing • • Live image comparison Best-fit measurements • Custom application builder • • Live tiling & Live EDF acquisition • Track moving objects • • Report & Share TWAIN acquisition Track merging & fading objects • • Annotate Measure intensity over time • Quick save for publication & analysis Auto calibration • • Export to Excel, PowerPoint & Word Collect data from multiple images & image frames • • Create automatic reports (including PDF) • Create snapshot of zoomed image • Create snapshot with overlays Process & Analyze • Post-acquisition alignment & EDF • Post-acquisition tiling • Image overlay & comparison tools • Fast Fourier Transform • Frame averaging Count & Classify • Color composite • • Pseudocolor • Image math • Classify objects • Filters – enhancement, edge, large, morphological, 3D • Smart Segmentation • Sort objects Automatic object splitting • Manually tag objects View Automatically find, count & measure objects • Binary mask • • Sequence/movie editing • Parent/child analysis • Bitmap analysis • Percent area analysis • High-dynamic-range processing • Count objects on multi-frame image • Overlay Features Manager • • Background subtraction/correction Measure distances between counted objects • Auto trace and magic wand • • Measure distance of objects from reference object ROI inverse (donut ROI) • Batch-process folders of images • Create Voronoi map to measure neighboring objects • Grid tool • Surface plot view & movie creator • Gallery view • Lock & zoom multiple images • Full-screen mode • 3D viewer & movie creator • Slicer view Supported File Formats Read Write TIFF Description *.tif, *.tiff • • Applied Precision® DeltaVision® *.dv • JPEG *.jpeg, *.jpg • • Image Cytometry *.ics • JPEG 2000 *.jp2, *.jpf • • Media Cybernetics Image-Pro Plus set *.ips • Microsoft Windows bitmap *.bmp • • Media Cybernetics set *.mcs • PNG file *.png • • Media Cybernetics AutoQuant *.deb • Raw binary data file *.raw • Micro-Manager *.metadatatext • Flat file *.flf • *.oif, *.oib • Flex. Image Transport System *.fts, *.fit, *.fits • *.nd, *.stk • OME-TIFF *.tiff • *.nd2 • Media Cybernetics Image-Pro sequence *.seq • • Carl Zeiss *.lsm, *.zvi • AVI movie file *.avi • • Leica® *.lei, *.lif • Microsoft Windows Media Video *.wmv • ® Aperio *.svs • StreamPix sequence *.seq • BigTIFF *.btf • *.tim • ® PerkinElmer Extension Description Extension ® ® ® Olympus FluoView FV1000 • ® ® Molecular Devices MetaMorph ® Nikon NIS-Elements ® Read Write • www.mediacy.com | [email protected] Exceptional Service & Support Training Our image analysis training courses provide valuable information you can apply towards your imaging research. Whether you are new to imaging or are a more experienced imaging professional, our courses are designed to help you make optimum use of the latest imaging software techniques. Get Started with Free Video Tutorials As an Image-Pro Premier user, you’ll have access to a growing library of free 3- to 5-minute video tutorials. Learn timesaving tips and familiarize yourself with the wide range of analysis tools available to you – without ever having to leave your computer. Image-Pro Users Email List When you choose Image-Pro software, you instantly join a community of image analysis professionals. Join the free Image-Pro users email list to ask questions and learn from other users. Share Tools on the User-Contributed Solutions Zone Website Explore our Solutions Zone website to find a wealth of useful information about Image-Pro Premier. The Solutions Zone is an easily searchable online database of macros, device drivers, plug-in modules, application programs, and development consultants that support Image-Pro Premier and other Media Cybernetics software programs. Technical Support Receive installation and configuration troubleshooting from our technical support engineers via phone or email. Purchase an Image-Pro Premier Maintenance Agreement to receive a full year of technical support and software updates. System Requirements Supported Operating Systems: Microsoft Windows XP® SP 3 and later – 32 bit Microsoft Windows® 7 Professional and Ultimate – 32 and 64 bit Minimum Requirements: • • • • • • Dual-core processor 4GBRAM 5GBof d-diskspace 1,024x768 display with 32-bit video card DVD-ROM/CD-ROM drive Full Microsoft.NET Framework 4.0 (automatically installed by application) • USB port required for hard licenses and offline license activation • Internet connection required for online services Recommended Requirements: • • • • • • • Four-or-more-core processor 64-bit operating system 16 GB RAM or more 20 GB or more of available hard-disk space 1,280x800 display with 1 GB video card DVD-ROM/CD-ROM drive Full Microsoft.NET Framework 4.0 (automatically installed by application) • USB port required for hard licenses and offline license activation • Internet connection required for online services Specifications are subject to change. Please contact Media Cybernetics or your local reseller for the latest features. FREE Video Tutorials Media Cybernetics, Inc. Tel: + 1 301-495-3305 Toll Free - North America: 800-263-2088 www.mediacy.com | [email protected] www.mediacy.com | [email protected] Copyright © 2012 Media Cybernetics, Inc. All rights reserved. Media Cybernetics, the Media Cybernetics logo, AutoQuant, and Image-Pro are registered trademarks of Media Cybernetics. Excel, Microsoft, PowerPoint, Windows, Windows XP, and Word are registered trademarks of Microsoft Corporation in the United States and other countries. All other brand and product names are the trademarks or registered trademarks of their respective owners and manufacturers.
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