Hanna Instruments HI254 pH/ORP Bench Meter with GLP

Hanna Instruments HI254 pH/ORP Bench Meter with GLP
Instruction Manual
HI 254
Microprocessor-Based
pH/mV/ºC
Bench Meter
w w w. h a n n a i n s t . c o m
1
Dear Customer,
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the instrument.
This manual will provide you with the necessary information for correct use of
the instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e-mail us at
tech@hannainst.com or turn to the back cover for our worldwide contact list.
This instrument is in compliance with
directives.
WARRANTY
HI 254 is guaranteed for two years against defects in workmanship and
materials when used for its intended purpose and maintained according to
instructions. Electrodes and probes are guaranteed for six months. This
warranty is limited to repair or replacement free of charge.
Damage due to accidents, misuse, tampering or lack of prescribed maintenance
is not covered.
If service is required, contact the dealer from whom you purchased the
instrument. If under warranty, report the model number, date of purchase,
serial number and the nature of the problem. If the repair is not covered by
the warranty, you will be notified of the charges incurred. If the instrument is
to be returned to Hanna Instruments, first obtain a Returned Goods Authorization number from the Technical Service department and then send it with
shipping costs prepaid. When shipping any instrument, make sure it is
properly packed for complete protection.
TABLE OF CONTENTS
WARRANTY ......................................................................................................................... 2
PRELIMINARY EXAMINATION .............................................................................................. 3
GENERAL DESCRIPTION ..................................................................................................... 3
FUNCTIONAL DESCRIPTION ................................................................................................ 4
HI 254 SPECIFICATIONS .................................................................................................... 5
OPERATIONAL GUIDE ......................................................................................................... 6
pH CALIBRATION ................................................................................................................ 9
GOOD LABORATORY PRACTICE (GLP) ...................................................................................12
LOGGING FUNCTION ..............................................................................................................14
SETUP .....................................................................................................................................17
TEMPERATURE CALIBRATION (for technical personnel only) ........................................... 21
mV CALIBRATION (for technical personnel only) .............................................................. 23
RELATIVE mV CALIBRATION .............................................................................................. 24
PC INTERFACE .................................................................................................................... 25
pH VALUES AT DIFFERENT TEMPERATURES .................................................................... 27
ELECTRODE CONDITIONING & MAINTENANCE .................................................................. 28
TROUBLESHOOTING GUIDE ............................................................................................... 31
TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS ............................................... 32
ACCESSORIES ...................................................................................................................... 33
2
PRELIMINARY EXAMINATION
Remove the instrument from the packing material and examine it carefully to
make sure that no damage has occurred during shipping. If there is any
damage, notify your Dealer or the nearest Hanna Customer Service Center.
Each instrument is supplied with:
• HI 1131B Glass-body Combination pH Electrode with 1 m (3.3') Cable
• HI 7669/2W Temperature Probe
• HI 76404 Electrode Holder
• pH 4.01 & 7.01 Buffer Solutions (20 mL each)
• HI 7071S Electrolyte Solution
• 12VDC Power Adapter
• Instruction Manual
Note: Save all packing material until you are sure that the instrument
functions correctly. All defective items must be returned in the original
packing with the supplied accessories.
GENERAL DESCRIPTION
The HANNA HI 254 is a logging microprocessor-based pH and temperature
bench meter.
HI 254 can also measure Oxidation Reduction Potential (ORP) in mV range.
Relative mV feature is also provided.
pH measurements are compensated for temperature effect manually or
automatically with the HI 7669/2W temperature probe.
Up to five-point calibration is possible, using seven memorized buffers (1.68,
4.01, 6.86, 7.01, 9.18, 10.01 and 12.45 pH).
The GLP feature provides a guarantee of data consistency.
An alarm time-out is available to alert the user that too much time elapsed
since the last pH calibration.
The instrument can store data in memory at the user’s request for later
retrieval.
Hanna Instruments reserves the right to modify the design, construction
and appearance of its products without advance notice.
3
FUNCTIONAL DESCRIPTION
Primary LCD
Secondary LCD
Front Panel
CAL LOG
1
% TIME DATE INTV
BUFFER pH C
mV pH
CFM
READY
WRONG BUF
10
2
Functions
3
4
CAL
CFM
GLP
5
Show GLP Data
Store Log Data
/
RCL
Recall Log Data
CFM
RANGE
6
C
C
LOG
RCL
pH Calibration - 5 points
GLP
LOG
SET/CLR
CAL
Select pH Buffer
RANGE
Confirm Calibration
in selected pH Buffer
Select pH/ORP/Rel mV
Scroll Log Data
Set Instrument Parameters
Clear Calibration/Log Data
7
9
Start/Exit Calibration
8
SET
CLR
HI 254
pH / ORP Meter
Rear Panel
POWER
12VDC
11
INPUT
TEMP
RS232
REF
12 13 14
15
1) Liquid Crystal Display (LCD).
2) CFM/GLP key, to confirm different values or to display Good Laboratory
Practice information.
3) CAL key, to enter and exit/escape calibration mode.
4) /ºC key, to manually decrease temperature value or other parameters.
5) /ºC key, to manually increase temperature value or other parameters.
6) RCL key, to enter/exit recall mode.
7) LOG key, to store measured data.
8) SET/CLR key, to enter/exit setup mode, clear pH calibration or delete
logged data.
9) RANGE key, to select measurement range or to switch focused data.
10) ON/OFF switch.
11) Power supply socket.
12) BNC electrode connector.
13) Electrode reference socket.
14) Temperature probe socket.
15) RS232 serial communication connector.
4
HI 254
SPECIFICATIONS
–2.0 to 16.0 p H
–2.00 to 16.00 p H
–2.000 to 16.000 p H
RANGE
±699.9 m V
±2000 m V
–9.9 to 120.0 ºC
0.1 p H
0.01 p H
0.001 p H
RE SOL UTION
0.1 m V (±699.9 m V)
1 m V (±2000 m V)
0.1 ºC
± 0.1 p H
± 0.01 p H
± 0.002 p H
ACCURACY
@ 20 °C/68 °F
± 0.2 m V (±699.9 m V)
± 1 m V (±2000 m V)
±0.4 ºC
(exclu d in g p rob e error)
Rel m V offset ra n g e
±2000 m V
Com p u ter in terfa ce
op to-isola ted RS232
p H Ca lib ra tion
1, 2, 3, 4 or 5 p oi n t ca l i b ra ti on ,
7 b u ffers a v a i l a b l e
(1.68, 4.01, 6.86, 7.01, 9.18,
10.01, 12.45)
Tem p era tu re
com p en sa tion
Ma n u a l or Au tom a tic from :
–9.9 to 120.0 ºC
p H E lectrod e
HI 1131B
Tem p era tu re p rob e
HI 7669/2W
In p u t im p ed a n ce
1012 oh m
Power su p p ly
12 VD C a d a p ter
D im en sion s
240x182x74 m m (9.4x7.1x2.9”)
Weig h t
1.1 K g (2.5 lb );
kit with h old er 2.5 K g (5.5 lb )
E n viron m en t
0 – 50 ºC (32 – 122 ºF)
m a x RH 95% n on con d en sin g
Wa rra n ty
2 yea rs
5
OPERATIONAL GUIDE
POWER CONNECTION
Plug the 12 VDC adapter into the power supply socket.
Note: This instrument uses non volatile memory to retain the pH, mV,
temperature calibrations and all other settings, even when unplugged.
Note: Make sure a fuse protects the main line.
ELECTRODE AND PROBE CONNECTIONS
For pH or ORP measurements connect an electrode with internal reference to
the BNC connector on the back of the instrument.
For electrodes with a separate reference connect the electrode’s BNC to the BNC
connector and the reference electrode plug to the reference socket.
For temperature measurements and automatic temperature compensation
connect the temperature probe to the appropriate socket.
INSTRUMENT START-UP
• Turn the instrument on by pressing the ON/OFF switch.
• All LCD tags are displayed and a beep is sounded while
the instrument performs a self test.
CAL LOG
% TIME DATE INTV
BUFFER pH C
mV pH
CFM
READY
WRONG BUF
pH MEASUREMENTS
Make sure the instrument has been calibrated before taking
pH measurements.
• Submerge the electrode tip and the temperature probe
approximately 4 cm (1½”) into the sample to be tested
and stir gently. Allow time for the electrode to stabilize.
• The pH is displayed on the primary LCD and the temperature on the
secondary LCD.
pH
C
• If the reading is out of range, the closest full-scale value will be displayed
blinking on the primary LCD.
pH
C
6
If measurements are taken successively in different samples, it is recommended
to rinse the electrode thoroughly with deionized water or tap water and then
with some of the next sample to prevent cross-contamination.
The pH reading is affected by temperature. In order to measure the pH
accurately, the temperature effect must be compensated for. To use the
Automatic Temperature Compensation feature, connect and submerge the
HI 7669/2W temperature probe into the sample as close as possible to the
electrode and wait for a few seconds.
If the temperature of the sample is known, simple manual compensation can
be performed by disconnecting the temperature probe.
The display will then show the default temperature of 25 ºC
or the last temperature reading with the“ºC” tag blinking.
The temperature can now be adjusted with the ARROW keys
(from –9.9 ºC to 120.0 ºC).
C
C
C
mV/ORP MEASUREMENTS
An optional ORP electrode must be used to perform ORP measurements
(see Accessories).
Oxidation-Reduction Potential (REDOX) measurements provide the quantification
of the oxidizing or reducing power of the tested sample.
To correctly perform a REDOX measurement, the surface of
the ORP electrode must be clean and smooth.
• Press RANGE to enter mV range.
• Submerge the tip of the ORP electrode 4 cm (1½”) into
the sample to be tested and allow a few seconds for the
reading to stabilize.
• The instrument displays the mV reading on the primary LCD and the
temperature on the secondary LCD.
mV
C
• If the reading is out of range, the closest full-scale value will be displayed
blinking on the primary LCD.
mV
C
7
RELATIVE mV MEASUREMENTS
• Press RANGE until “rEL” message will be displayed on the secondary LCD line
for about one second and “mV” tag will blink. After one second the
temperature will be displayed on the secondary LCD.
mV
C
The reading displayed by the instrument is equal to the difference between
the current mV input value and relative mV offset established in the relative
mV calibration.
TEMPERATURE MEASUREMENTS
Connect the HI 7669/2W temperature probe to the appropriate
socket and turn the instrument on.
Immerse the temperature probe into the sample and allow
the reading on the secondary LCD to stabilize.
8
pH CALIBRATION
Calibrate the instrument frequently, especially if high accuracy is required.
The instrument should be recalibrated :
• Whenever the pH electrode is replaced.
• At least once a week.
• After testing aggressive chemicals.
• If “CAL” “INTV” tags are blinking during measurement.
Every time you calibrate the instrument use fresh buffers and perform an
electrode Cleaning Procedure (see page 30).
PREPARATION
Pour small quantities of the buffer solutions into clean beakers. If possible, use
plastic or glass beakers to minimize any EMC interferences.
For accurate calibration and to minimize cross-contamination, use two beakers
for each buffer solution. One for rinsing the electrode and one for calibration.
If you are measuring in the acidic range, use pH 7.01 or 6.86 as first buffer and
pH 4.01 or pH 1.68 as second buffer. If you are measuring in the alkaline range,
use pH 7.01 or 6.86 as first buffer and pH 10.01 or pH 12.45 as second buffer.
PROCEDURE
Calibration has a choice of 7 memorized buffers: pH 1.68, 4.01, 6.86, 7.01,
9.18, 10.01 and 12.45.
It is recommended to perform at least a two-point calibration. However, the
instrument allows up to five-point calibration, as described on page 11.
ONE-POINT CALIBRATION
• Immerse the pH electrode and the temperature probe
approximately 4 cm (1½”) into a buffer solution of
your choice (pH 1.68, 4.01, 6.86, 7.01, 9.18, 10.01
or 12.45) and stir gently. The temperature probe
should be close to the pH electrode.
• Press CAL. The “CAL” and “ ” tags will appear
and the “7.01” buffer will be displayed on the
secondary LCD.
BUF
CAL
pH
BUFFER pH
BUF
• If necessary, press the ARROW keys to select a different buffer value.
9
• The “ ” tag will blink on the LCD until the reading is
stable.
• When the reading is stable and close to the selected buffer, the
“READY” tag will be displayed and the “CFM” tag will blink.
• Press CFM to confirm calibration.
• The calibrated value will be displayed on the primary LCD
and the second expected buffer value on the secondary
LCD.
CAL
pH
BUFFER pH
BUF
• Press CAL to quit calibration. The instrument will return
to measurement mode and will memorize the one-point
calibration data.
TWO-POINT CALIBRATION
Any two of the 7 memorized buffers can be used for this purpose. However, it
is recommended that pH 6.86 or 7.01 to be chosen as first calibration point
and pH 4.01 (acidic) or pH 9.18/10.01 (alkaline) as second calibration point.
• Proceed as described in “One-point calibration” section, but do not quit
calibration by pressing CAL at the end.
• After the first calibration point is confirmed, immerse the pH electrode and
the temperature probe approximately 4 cm (1½”) into the second buffer
solution and stir gently. The temperature probe should be close to the pH
electrode.
• If necessary, press the ARROW keys to select a different buffer value.
• The “ ” tag will blink on the LCD until the reading is
stable.
• When the reading is stable and close to the selected buffer, the
“READY” tag will be displayed and the “CFM” tag will blink.
• Press CFM to confirm calibration.
• Press CAL to quit calibration. The instrument will return
to measurement mode and will memorize the one-point
calibration data.
THREE TO FIVE-POINT CALIBRATION
Any of the unused buffers in previous calibration can be used for this purpose.
• Proceed as described in “Two-point calibration” section, but do not quit
calibration by pressing CAL at the end.
• After the second calibration point is confirmed, immerse the pH electrode
and the temperature probe approximately 4 cm (1½”) into the next
buffer solution and stir gently. The temperature probe should be close to
the pH electrode.
10
• If necessary, press the ARROW keys to select a different buffer value.
• The “ ” tag will blink on the LCD until the reading is
stable.
• When the reading is stable and close to the selected buffer, the
“READY” tag will be displayed and the “CFM” tag will blink.
• Press CFM to confirm calibration.
• If the fifth calibration point is reached, the instrument
stores the calibration value and returns to normal measurement mode, or else it goes to the next calibration point.
Note: • If the value measured by the instrument is not close to the
selected buffer, “WRONG” “ ” and “WRONG” “ ” tags will
blink alternately. In this case, check if the correct buffer has been
used or regenerate the electrode by following the Cleaning Procedure (see page 30). If necessary, change the buffer or the
electrode.
• If “WRONG”, “Buffer pH” tags and “Old” tag on the secondary LCD
are displayed blinking, an inconsistency between new and previous
(old) calibration is detected. Clear calibration parameters by
pressing SET/CLR and proceed with calibration from the current
calibration point (the instrument will keep all confirmed values
during current calibration).
• The “WRONG” tag and temperature value are displayed blinking
if temperature reading is out of the defined temperature range of
the buffer. Calibration cannot be confirmed in this situation.
• Press RANGE to toggle between pH buffer, buffer
number and temperature reading on the secondary
LCD during calibration.
Note: Calibration parameters are evaluated taking in consideration the new
values for the confirmed buffers in current calibration and the old
values, if existing, for the other buffers. To clear old calibration values,
press SET/CLR before exiting calibration.
BUF
11
GOOD LABORATORY PRACTICE (GLP)
GLP is a set of functions that allows storage and retrieval of data regarding the
maintenance and status of the electrode.
All data regarding the last calibration (one, two, three, four or five-point) is
stored for the user to review when necessary. This data includes the following:
calibration time stamp, offset (mV), average of slope (%), calibration buffers
and the time until a new calibration is required.
CALIBRATION ALARM TIME-OUT
HI 254 allows the user to set the number of days before the next required
calibration. This value can be set from 1 to 7 days. The default setting is OFF
(disabled).
The instrument checks if the time-out time has expired. If the time elapsed, the
“CAL” “INTV” tags will blink as a reminder.
Note: If the instrument was not calibrated, the “CAL” “INTV” tags will be
displayed even if the feature is disabled in SETUP menu.
LAST CALIBRATION DATA
Last calibration data is stored automatically after a successful calibration.
To view the pH calibration data, press GLP while in measurement mode.
The instrument will display the time (hh:mm) of the last calibration.
TIME
CAL
Press the ARROW keys to view the next logged calibration parameter
(pressing the ºC key):
• The date (yyyy.mm.dd).
DATE
CAL
• The pH calibration offset.
CAL
mV
• The pH calibration slope (the GLP slope is the average of the calibration
slopes; the percentage is referred to the ideal value of 59.16 mV/pH).
%
CAL
12
• The pH calibration buffers in calibrating order.
The first pH calibration buffer:
CAL
pH
BUF
The second pH calibration buffer:
CAL
pH
BUF
The third pH calibration buffer:
CAL
pH
BUF
The fourth pH calibration buffer:
CAL
pH
BUF
The fifth pH calibration buffer:
CAL
pH
BUF
Note: •
The “OLd” message displayed beside the pH value means that
this buffer was not used during last calibration. Press and hold
down SET/CLR if you want to see calibration date (or time if old
calibration was made in the same day with current calibration).
• If “no bUF” message appears on the LCD, the instrument informs
you that calibration was performed in less than five points.
CAL
BUF
• pH resolution during calibration.
CAL
• Calibration Alarm Time-Out status:
if disabled.
13
or the number of days until the calibration alarm will be displayed.
or if expired (7 days ago).
INTV
CAL
• The instrument ID.
Note: •
Press GLP at any moment and the instrument will return to
measurement mode.
• If calibration has not been performed, the instrument displays
“no CAL” message blinking.
CAL
LOGGING FUNCTION
Up to 50 LOG samples can be stored into memory.
LOGGING THE CURRENT DATA
To store the current reading into memory press LOG while the instrument is in
measurement mode.
The instrument will display the current date (mm.dd) on the primary LCD, the
record number on the secondary LCD and “LOG” tag will blink for a few seconds
(see example below: record No. 5 dated September 14):
LOG
If there are less than 6 memory locations remaining, the record number and
“Lo” message will blink to alert the user.
LOG
If the log space is full, “FULL LOC” message will be displayed and no more
data will be saved.
LOG
14
When LOG is pressed, a complete set of information is stored: date, time, pH,
mV, temperature and calibration data.
VIEW LOGGED DATA
Press RCL while in measurement mode to retrive the stored information.
If no data were logged, the instrument displays:
LOG
Otherwise, the instruments will display the pH or relative mV value on the
primary LCD and the last stored record number on the secondary LCD, along
with “LOG” tag.
LOG
pH
or
LOG
mV
Pressing the ARROW keys, the instrument will display the same parameter
but for a different record.
LOG
pH
Press RANGE and the instrument will display the next logged parameter as
follows:
• The mV value on the primary LCD and the record number on the secondary
LCD.
LOG
mV
• The temperature value on the primary LCD and the record number on
the secondary LCD.
LOG
C
Note: When the pH, mV or temperature is displayed, if SET/CLR is pressed
and hold down, the secondary LCD will display the record date.
15
• The time on the primary LCD, along with “TIME” tag.
TIME
LOG
• The date on the primary LCD, along with “DATE” tag.
DATE
LOG
• The calibration offset on the primary LCD and “OFS” message on the
secondary LCD.
LOG
mV
• The calibration slope on the primary LCD and “SLP” message on the
secondary LCD.
%
LOG
Note: • If in Relative mV RECALL mode, the instruments will display “----”
message on the primary LCD.
• When the time, date, year, offset or slope is displayed, if SET/CLR
is pressed and hold down, the secondary LCD will display the record
number.
• The “dEL” message on the primary LCD and the selected record on the
secondary LCD, along with ”LOG” tag.
LOG
Note: • If one of the ARROW keys is pressed while “dEL” and record
number is displayed, the next/previous record number is selected.
• If LOG is pressed, the secondary LCD will display “ALL” message.
LOG
• Pressing LOG again, the secondary LCD will display the record
number.
• Press SET/CLR to delete the selected or all records.
16
• If “del ALL” option was selected, all records are deleted and the
instrument returns to measurement mode.
Note: After LOG is pressed while in measurement mode or a selected record is
deleted, the instrument will display the amount of free log space for about
one second (e.g. 25 records free).
LOG
Press RCL to leave RECALL mode at any time.
SETUP
Setup mode allows viewing and modifying the following parameters:
• Current Time
• Current Date
• pH Resolution
• Beep Status
• Baud Rate (serial communication)
• Command prefix (serial communication)
• Calibration Alarm Time-Out
• Instrument ID
To enter SETUP mode press SET while the instrument is in measurement mode.
Select a parameter with the ARROW keys.
Press CAL if you want to change a parameter value. The selected parameter
will start blinking.
Press RANGE to toggle between displayed parameters.
Press the ARROW keys to increase or decrease the displayed value.
Press CFM to save the modified value or CAL to escape.
Press the ARROW keys to select the next/previous parameter.
CURRENT TIME SET
Press CAL when the current time is displayed. The hour will start blinking.
TIME
Press the ARROW keys to change the hour.
Press RANGE. The minutes will start blinking.
TIME
17
Press the ARROW keys to change the minutes.
Press CFM to save the modified value.
Press CAL to escape without saving.
CURRENT DATE SET
Press CAL when the current date is displayed. The year will start blinking.
DATE
Press the ARROW keys to change the year.
Press RANGE. The month will start blinking.
DATE
Press the ARROW keys to change the month.
Press RANGE. The day will start blinking.
DATE
Press the ARROW keys to change the day.
Press CFM to save the modified value.
Press CAL to escape without saving.
pH RESOLUTION SET
Press CAL when the pH range is displayed. The pH range (0.1, 0.01 or
0.001) will start blinking.
pH
Press the ARROW keys to change the pH range.
Press CFM to save the modified pH range.
Press CAL to escape without saving.
18
BEEP STATUS SET
Press CAL when the beep status is displayed. Beep status (On or OFF) will
start blinking.
Press the ARROW keys to change the beep status (On or OFF).
Press CFM to save the modified beep status.
Press CAL to escape without saving.
BAUD RATE SET
Press CAL when the baud rate is displayed. The baud rate (600, 1200, 2400,
4800 or 9600) will start blinking.
Press the ARROW keys to change the baud rate value.
Press CFM to save the modified baud rate value.
Press CAL to escape without saving.
SERIAL COMMUNICATION COMMAND PREFIX SET
Press CAL when the command prefix is displayed. The command prefix (0 to
47) will start blinking.
Press the ARROW keys to change the command prefix.
Press CFM to save the modified command prefix value.
Press CAL to escape without saving.
Note: • See the PC interface section on page 25 for a complete explanation.
• The command prefix must be 16 if HI 92000 PC software is used.
CALIBRATION ALARM TIME-OUT SET
Press CAL when the calibration time-out is displayed. Calibration time-out
(OFF or 1 to 7 days) will start blinking.
INTV
CAL
19
Press the ARROW keys to change the calibration time-out value.
Press CFM to save the modified calibration time-out value.
Press CAL to escape without saving.
INSTRUMENT ID PARAMETER SET
Press CAL when the instrument ID is displayed. The instrument ID (0000 to
9999) will start blinking.
Press the ARROW keys to change the instrument ID value.
Press CFM to save the modified instrument ID value.
Press CAL to escape without saving.
Note: The instrument ID is downloaded to a PC as part of a logged data, set
to identify its origin.
20
TEMPERATURE CALIBRATION
(for technical personnel only)
All the instruments are factory calibrated for temperature.
Hanna’s temperature probes are interchangeable and no temperature
calibration is needed when they are replaced.
If the temperature measurements are inaccurate, temperature recalibration
should be performed.
For an accurate recalibration, contact your dealer or the nearest Hanna
Customer Service Center, or follow the instructions bellow.
• Prepare a vessel containing ice and water and another one containing hot
water (at a temperature of around 50 ºC). Place insulation material
around the vessels to minimize temperature changes.
• Use a calibrated thermometer with a resolution of 0.1 ºC as a reference
thermometer.
• With the instrument off, press and hold down the CAL&LOG keys, then
power on the instrument. The “CAL” tag will appear and the secondary
LCD will show 0.0 ºC.
CAL
C
• Immerse the temperature probe in the vessel with ice and water as near
as possible to the reference thermometer. Allow a few seconds for the probe
to stabilize.
• Use the ARROW keys to set the reading on the secondary LCD to that of
ice and water, measured by the reference thermometer. When the reading
is stable and close to the selected calibration point, “READY” tag will
appear and “CFM” tag will blink.
• Press CFM to confirm. The secondary LCD will show 50.0 ºC.
CAL
C
• Immerse the temperature probe in the second vessel as near as possible to
the reference thermometer. Allow a few seconds for the probe to stabilize.
21
• Use the ARROW keys to set the reading on the secondary LCD to that of
the hot water.
C
C
• When the reading is stable and close to the selected
calibration point, “READY” tag will appear and “CFM”
tag will blink.
• Press CFM to confirm. The instrument returns to
measurement mode.
Note: If the reading is not close to the selected calibration point, “WRONG”
tag will blink. Change the temperature probe and restart calibration.
22
mV CALIBRATION
(for technical personnel only)
All the instruments are factory calibrated for mV.
Hanna’s ORP electrodes are interchangeable and no mV calibration is
needed when they are replaced.
If the mV measurements are inaccurate, mV recalibration should be performed.
For an accurate recalibration, contact your dealer or the nearest Hanna
Customer Service Center, or follow the instructions below.
A two or three-point calibration can be performed at 0.0 mV, 600.0 mV and
1800.0 mV.
• Attach to the BNC connector a mV simulator with an accuracy of ±0.1 mV.
• With the instrument off, press and hold down the CFM&RCL keys, then
power on the instrument. The “CAL” tag will appear and the secondary
LCD will show 0.0 mV.
• Set 0.0 mV on the simulator.
When the reading is stable and close to the selected calibration point,
“READY” tag will appear and “CFM” tag will blink.
• Press CFM to confirm. The secondary LCD will display 600 mV.
• Set 600.0 mV on the simulator.
When the reading is stable and close to the selected calibration point,
“READY” tag will appear and “CFM” tag will blink.
• Press CFM to confirm. The secondary LCD will display 1800 mV.
• Set 1800.0 mV on the simulator.
When the reading is stable and close to the selected calibration point,
“READY” tag will appear and “CFM” tag will blink.
• Press CFM to confirm. The instrument returns to measurement mode.
Note: •
•
If the reading is not close to the selected calibration point,
“WRONG” tag will blink. Verify calibration condition or contact
your vendor if you can not calibrate.
Press CAL in any moment of the calibration process. The instrument
will return to measurement mode. If calibration process is stopped
after 600 mV is confirmed, the 600 mV range is calibrated and
calibration parameters are memorized.
23
RELATIVE mV CALIBRATION
• Press CAL when the instrument is in RELATIVE mV measurement mode.
The “mV” and “ ” tags will blink. Absolute mV is displayed on the
primary LCD and “AbS” message is displayed on the secondary LCD.
• When the absolute reading is stable and in measurement range, the
instrument asks for confirmation.
• If the reading is out of range, “WRONG” tag will be displayed.
• Press CFM to confirm the absolute value. The instrument will display 0.0
mV on the primary LCD and “rEL” message on the secondary LCD. In this
moment the relative mV offset is equal to absolute mV reading.
• Use the ARROW keys if you want to change the displayed relative mV value.
• Press CFM to confirm the relative mV value. The relative mV offset is
displayed on the primary LCD and “OFF” message on the secondary LCD for
a few seconds. The instrument returns to measurement mode.
Note: The relative mV value can be changed only inside the relative mV offset
window (± 2000 mV).
24
PC INTERFACE
Data transmission from the instrument to the PC can be done with the HI
92000 Windows® compatible software (optional). HI 92000 also offers
graphing and on-line help feature.
Data can be exported to the most popular spreadsheet programs for further
analysis.
To connect your instrument to a PC, use the optional Hanna HI 920010 cable
connector. Make sure that your instrument is switched off and plug one
connector to the instrument RS232C socket and the other into the serial port
of your PC.
Note: • Other cables than HI 920010 may use a different configuration.
In this case communication between instrument and PC may not
be possible.
• If you are not using Hanna Instruments HI 92000 software,
please see the following instructions.
SENDING COMMANDS FROM PC
It is also possible to remotely control the instrument with any terminal
program. Use HI 920010 cable to connect the instrument to a PC, start the
terminal program and set the communication options as follows: 8, N, 1, no
flow control.
COMMAND TYPES
To send a command to the instrument the scheme is:
<command prefix> <command> <CR>
where:
<command prefix> is a selectable ASCII character
between 0 and 47.
<command> is the command code (3 characters).
Note: Either small or capital letters can be used.
SIMPLE COMMANDS
RNG
CAL
CFM
UPC
DWC
SET
LOG
Is equivalent to pressing RANGE
Is equivalent to pressing CAL
Is equivalent to pressing CFM/GLP
Is equivalent to pressing the UP arrow key
Is equivalent to pressing the DOWN arrow key
Is equivalent to pressing SET/CLR
Is equivalent to pressing LOG
25
CHR n
Change the instrument range according with the parameter
value (n):
• n=0 pH range/0.001 resolution
• n=1 pH range/0.01 resolution
• n=2 pH range/0.1 resolution
• n=3 mV range
• n=4 Relative mV range
COMMANDS REQUIRING AN ANSWER
pH?
MV?
MR?
TM?
RAS
MDR
PAR
NSL
GLP
LOD xxx
LOD ALL
Note: •
•
•
Causes the instrument to send the pH reading. If the range is set
to mV, “Err6” is received.
Causes the instrument to send the mV reading. If the range is set
to pH, “Err6” is received.
Causes the instrument to send the relative mV reading. If the
range is set to pH or mV, “Err6” is received.
Causes the instrument to send the temperature reading.
Causes the instrument to send a complete set of readings in
according with the current range:
• pH, mV and temperature reading on pH range.
• mV and temperature reading on mV range.
• Rel mV, absolute mV and temperature reading on Rel
mV range.
Requests the instrument model name and firmware code.
Requests the setup parameters setting (instrument ID, calibration alarm time-out, pH range, beep status).
Requests the number of logged samples.
Requests the calibration data record.
Requests the xxxth record logged data (“Err3” is sent when “xxx”
is an invalid record number).
Requests all logged data.
“Err8” is sent if instrument is not in measurement mode.
“Err7” is sent if mV are asked during pH calibration mode.
“NAK” (21) character is sent when the instrument receives an
unknown or a corrupted command.
26
pH VALUES AT DIFFERENT
TEMPERATURES
The temperature has an effect on pH. The calibration buffer solutions are
affected by temperature changes to a lesser degree than normal solutions.
During calibration the instrument will automatically calibrate to the pH value
corresponding to the measured or set temperature.
TEMP
pH VALUES
ºC
ºF
1.68 4.01 6.86 7.01 9.18
10.01 12.45
0
32
1.67 4.01 6.98 7.13 9.46
10.32
13.42
5
41
1.67 4.00 6.95 7.10 9.39
10.24
13.21
10
50
1.67 4.00 6.92 7.07 9.33
10.18
13.00
15
59
1.67 4.00 6.90 7.04 9.27
10.12
12.81
20
68
1.68 4.00 6.88 7.03 9.22
10.06
12.63
25
77
1.68 4.01 6.86 7.01 9.18
10.01 12.45
30
86
1.68 4.02 6.85 7.00 9.14
9.96
12.29
35
95
1.69 4.03 6.84 6.99 9.10
9.92
12.13
40
104
1.69 4.04 6.84 6.98 9.07
9.98
11.98
45
113
1.70 4.05 6.83 6.98 9.04
9.85
11.84
50
122
1.71 4.06 6.83 6.98
9.01
9.82
11.70
55
131
1.72 4.07 6.84 6.98 8.99
9.79
11.57
60
140
1.72 4.09 6.84 6.98 8.97
9.77
11.45
65
149
1.73 4.11 6.85 6.99 8.95
9.76
-
70
158
1.74 4.12 6.85 6.99 8.93
9.75
-
During calibration the instrument will display the pH buffer value at 25 ºC.
27
ELECTRODE CONDITIONING
& MAINTENANCE
PREPARATION PROCEDURE
Remove the protective cap of the pH electrode.
DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT. This is normal with
electrodes. They will disappear when rinsed with water.
During transport, tiny bubbles of air may form inside the glass bulb affecting
proper functioning of the electrode. These bubbles can be removed by
“shaking down” the electrode as you would do with a glass thermometer.
If the bulb and/or junction is dry, soak the electrode in HI 70300 or HI 80300
Storage Solution for at least one hour.
28
For refillable electrodes:
If the filling solution (electrolyte) is more than 2½ cm (1”) below the fill hole,
add HI 7082 or HI 8082 3.5M KCl Electrolyte Solution for double junction or
HI 7071 or HI 8071 3.5M KCl+AgCl Electrolyte Solution for single junction
electrodes.
For faster response, unscrew the fill hole screw during measurements.
For AMPHEL ® electrodes:
If the electrode does not respond to pH changes, the battery is run down and
the electrode should be replaced.
MEASUREMENT
Rinse the electrode tip with distilled water. Immerse the tip (bottom 4 cm /1½”)
in the sample and stir gently for a few seconds.
For a faster response and to avoid cross-contamination of the samples, rinse
the electrode tip with a few drops of the solution to be tested, before taking
measurements.
STORAGE PROCEDURE
To minimize clogging and assure a quick response time, the glass bulb and
the junction should be kept moist and not allowed to dry out.
Replace the solution in the protective cap with a few drops of HI 70300 or
HI 80300 Storage Solution or, in its absence, Filling Solution (HI 7071 or
HI 8071 for single junction and HI 7082 or HI 8082 for double junction
electrodes). Follow the Preparation Procedure on page 28 before taking
measurements.
Note: NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.
PERIODIC MAINTENANCE
Inspect the electrode and the cable. The cable used for connection to the
instrument must be intact and there must be no points of broken insulation on
the cable or cracks on the electrode stem or bulb. Connectors must be perfectly
clean and dry. If any scratches or cracks are present, replace the electrode.
Rinse off any salt deposits with water.
For refillable electrodes:
Refill the reference chamber with fresh electrolyte (HI 7071 or HI 8071 for
single junction and HI 7082 or HI 8082 for double junction electrodes). Allow
the electrode to stand upright for 1 hour.
Follow the Storage Procedure above.
29
CLEANING PROCEDURE
• General
Soak in Hanna HI 7061 or HI 8061 General
Cleaning Solution for approximately ½ hour.
• Protein
Soak in Hanna HI 7073 or HI 8073 Protein
Cleaning Solution for 15 minutes.
• Inorganic
Soak in Hanna HI 7074 Inorganic Cleaning
Solution for 15 minutes.
• Oil/grease
Rinse with Hanna HI 7077 or HI 8077 Oil and Fat
Cleaning Solution.
IMPORTANT: After performing any of the cleaning procedures, rinse the
electrode thoroughly with distilled water, refill the reference chamber with
fresh electrolyte (not necessary for gel-filled electrodes) and soak the electrode
in HI 70300 or HI 80300 Storage Solution for at least 1 hour before taking
measurements.
30
TROUBLESHOOTING GUIDE
SYMPTOMS
Slow reponse/excessive
drift.
PROBLEM
D irty p H electrod e.
Rea d ing s fluctua te up Clog g ed /d irty junction.
a nd d own (noise).
L ow electrolyte level
(refilla b le electrod es
only).
Out of ra ng e in the
mV sca le.
D ry
memb ra ne/junction.
The meter d oes not
a ccep t the b uffer
solution for
ca lib ra tion.
Out of ord er p H
electrod e.
If the d isp la y shows:
"p H" a nd " -2.00" or
"16.00" b linking .
If the d isp la y shows:
"mV" a nd "-2000" or
"+2000" b linking
The meter d oes not
work with the
temp era ture p rob e.
The meter fa ils to
ca lib ra te or g ives
fa ulty rea d ing s.
At sta rtup the meter
d isp la ys a ll L CD ta g s
p erma nently.
SOLUTION
Soa k the electrod e tip in
HI 7061 or HI 8061
solution for 30 minutes
a nd then clea n the
electrod e.
Clea n the electrod e.
Refill with fresh solution
(for refilla b le electrod es
only).
Soa k in HI 70300 or
HI 80300 stora g e
solution.
Follow the clea ning
p roced ure. If still no
results, rep la ce the
electrod e.
Out of ra ng e in the p H a) Recalibrate the meter.
sca le.
b ) Ma ke sure the p H
sa mp le is in the sp ecified
ra ng e.
c) Check the electrolyte
level a nd the g enera l
sta te of the electrod e.
Out of ra ng e in the
mV sca le.
E lectrod e not connected .
Broken temp era ture
p rob e.
Rep la ce the temp era ture
p rob e.
Broken p H electrod e.
Rep la ce the electrod e.
One of the keys is
b locked .
Check the keyb oa rd or
conta ct the vend or.
"E rr xx" error messa g e Interna l error.
d isp la yed .
31
Power off the meter a nd
then p ower it on. If the
error p ersists, conta ct the
vend or.
TEMPERATURE CORRELATION
FOR pH SENSITIVE GLASS
The resistance of glass electrodes partially depends on the temperature. The
lower the temperature, the higher the resistance. It takes more time for the
reading to stabilize if the resistance is higher. In addition, the response time
will suffer to a greater degree at temperatures below 25 ºC.
Since the resistance of the pH electrode is in the range of 50 – 200 Mohm, the
current across the membrane is in the pico Ampere range. Large currents can
disturb the calibration of the electrode for many hours.
For these reasons high humidity environments, short circuits and static
discharges are detrimental to a stable pH reading.
The pH electrode’s life also depends on the temperature. If constantly used at
high temperatures, the electrode life is drastically reduced.
Typical Electrode Life
Ambient Temperature
1 – 3 years
90 ºC
Less than 4 months
120 ºC
Less than 1 month
Alkaline Error
High concentrations of sodium ions interfere with readings in alkaline
solutions. The pH at which the interference starts to be significant depends
upon the composition of the glass. This interference is called alkaline error and
causes the pH to be underestimated. Hanna’s glass formulations have the
indicated characteristics.
Sodium Ion Correction for the Glass at 20-25 ºC
Concentration
pH
Error
13.00
0.10
0.1 Mol L-1 Na+
13.50
0.14
14.00
0.20
12.50
0.10
13.00
0.18
1.0 Mol L-1 Na+
13.50
0.29
14.00
0.40
32
ACCESSORIES
pH BUFFER SOLUTIONS
HI 70004P
HI 70007P
HI 70010P
HI 7001L
HI 7004L
HI 7006L
HI 7007L
HI 7009L
HI 7010L
HI 7001L
HI 8004L
HI 8006L
HI 8007L
HI 8009L
HI 8010L
pH 4.01 Buffer Sachets, 20 mL, 25 pcs
pH 7.01 Buffer Sachets, 20 mL, 25 pcs
pH 10.01 Buffer Sachets, 20 mL, 25 pcs
pH 1.68 Buffer Solution, 500 mL
pH 4.01 Buffer Solution, 500 mL
pH 6.86 Buffer Solution, 500 mL
pH 7.01 Buffer Solution, 500 mL
pH 9.18 Buffer Solution, 500 mL
pH 10.01 Buffer Solution, 500 mL
pH 1.68 Buffer Solution in FDA approved bottle, 500 mL
pH 4.01 Buffer Solution in FDA approved bottle, 500 mL
pH 6.86 Buffer Solution in FDA approved bottle, 500 mL
pH 7.01 Buffer Solution in FDA approved bottle, 500 mL
pH 9.18 Buffer Solution in FDA approved bottle, 500 mL
pH 10.01 Buffer Solution in FDA approved bottle, 500 mL
ELECTRODE STORAGE SOLUTIONS
HI 70300L Storage Solution, 460 mL
HI 80300L Storage Solution in FDA approved bottle, 460 mL
ELECTRODE CLEANING SOLUTIONS
HI 70000P
HI 7061L
HI 7073L
HI 7074L
HI 7077L
HI 8061L
HI 8073L
HI 8077L
Electrode Rinse Sachets, 20 mL, 25 pcs
General Cleaning Solution, 460 mL
Protein Cleaning Solution, 460 mL
Inorganic Cleaning Solution, 460 mL
Oil & Fat Cleaning Solution, 460 mL
General Cleaning Solution in FDA approved bottle, 460 mL
Protein Cleaning Solution in FDA approved bottle, 460 mL
Oil & Fat Cleaning Solution in FDA approved bottle, 460 mL
HI 7071
3.5M KCl + AgCl Electrolyte, 4x30 mL, for single junction
electrodes
1M KNO3 Electrolyte, 4x30 mL
3.5M KCl Electrolyte, 4x30 mL, for double junction electrodes
3.5M KCl + AgCl Electrolyte in FDA approved bottle, 4x30 mL,
for single junction electrodes
1M KNO3 Electrolyte in FDA approved bottle, 4x30 mL
3.5M KCl Electrolyte in FDA approved bottle, 4x30 mL, for
double junction electrodes.
ELECTRODE REFILL ELECTROLYTE SOLUTIONS
HI 7072
HI 7082
HI 8071
HI 8072
HI 8082
33
ORP PRETREATMENT SOLUTIONS
HI 7091L
Reducing Pretreatment Solution, 460 mL
HI 7092L
Oxidizing Pretreatment Solution, 460 mL
pH ELECTRODES
All electrodes part numbers ending in B are supplied with a BNC connector
and 1 m (3.3') cable, as shown below :
HI 1043B
Glass-body, double junction, refillable, combination pH electrode.
Use: strong acid/alkali.
9.5mm DIA
0.37"
12 mm
0.5"
HI 1043
120 mm
4.7"
"S" VERSION
HI 1053B
Glass-body, triple ceramic, conic shape, refillable, combination pH electrode.
Use: emulsions.
HI 1040
12 mm
0.5"
HI 1053
120 mm
4.7"
"S" VERSION
HI 1083B
Glass-body, micro, Viscolene, non-refillable, combination pH electrode.
Use: biotechnology, micro titration.
HI 1050
12 mm
0.5"
5 mm
0.2"
3 mm
0.12"
3.0 mm DIA
0.12"
HI 1083
120 mm
4.7"
HI 1131B
Glass-body, single junction, refillable, combination pH electrode.
Use: general purpose.
9.5mm DIA
0.37"
HI 1131
"S" VERSION
120 mm
4.7"
34
HI 1111
12 mm
0.5"
HI 1330B
Glass-body, semimicro, single junction, refillable, combination pH electrode.
Use: laboratory, vials.
5mm DIA
0.2"
5mm
0.2"
HI 1330
120 mm
4.7"
"S" VERSION
HI 1331B
Glass-body, semimicro, single junction, refillable, combination pH electrode.
Use: flasks.
HI 1310
8 mm
0.3"
7.5mm DIA
0.29"
HI 1331
210 mm
8.25"
"S" VERSION
HI 1230B
Plastic-body (Ultem®), double junction, gel-filled, combination pH electrode.
Use: general, field.
HI 1311
12 mm
0.5"
HI 1230
120 mm
4.7"
"S" VERSION
HI 2031B
Glass-body, semimicro, conic, refillable, combination pH electrode.
Use: semisolid products.
HI 1210
6 mm
0.25"
HI 2031
75 mm
2.95"
"S" VERSION
HI 1332B
Plastic-body (Ultem®), double junction, refillable, combination pH electrode.
Use: general purpose.
12 mm
0.5"
HI 2020
HI 1332
"S" VERSION
120 mm
4.7"
35
HI 1312
FC 100B
Plastic-body (Kynar®), double junction, refillable, combination pH electrode.
Use: general purpose for food industry.
12 mm
0.5"
FC 100
120 mm
4.7"
FC 200B
Plastic-body (Kynar®), open junction, conic, Viscolene, non-refillable,
combination pH electrode. Use: meat & cheese.
6 mm
0.25"
FC 200
75 mm
2.95"
FC 210B
Glass-body, double junction, conic, Viscolene, non-refillable, combination
pH electrode. Use: milk, yogurt.
12 mm
0.5"
FC 210
120 mm
4.7"
FC 220B
Glass-body, triple-ceramic, single junction, refillable, combination pH
electrode. Use: food processing.
9.5mm DIA
0.37"
12 mm
0.5"
FC 220
120 mm
4.7"
FC 911B
Plastic-body (Kynar®), double junction, refillable with built-in amplifier,
combination pH electrode. Use: very high humidity.
12 mm
0.5"
FC 911
110 mm
4.3"
36
HI 1413B
Glass-body, single junction, flat tip, Viscolene, non-refillable, combination
pH electrode. Use: surface measurement.
12 mm
0.5"
HI 1413
110 mm
4.3"
ORP ELECTRODES
HI 3131B
Glass-body, refillable, combination platinum ORP electrode.
Use: titration.
12 mm
0.5"
HI 3131
150 mm
5.9"
"S" VERSION
HI 3230B
Plastic-body (Ultem®), gel-filled, combination platinum ORP electrode.
Use: general purpose.
HI 3111
12 mm
0.5"
HI 3230
120 mm
4.7"
"S" VERSION
HI 4430B
Plastic-body (Ultem®), gel-filled, combination gold ORP electrode.
Use: general purpose.
HI 3210
12 mm
0.5"
HI 4430
120 mm
4.7"
"S" VERSION
Consult the Hanna General Catalog for more electrodes with screw-type or
BNC connectors.
HI 4410
Ultem® is registered Trademark of “General Electric Co.”
Kynar® is registered Trademark of “Pennwalt Corp.”
37
EXTENSION CABLE FOR SCREW-TYPE ELECTRODES
(SCREW TO BNC ADAPTER)
HI 7855/1 Extension cable 1 m (3.3') long
HI 7855/3 Extension cable 3 m (9.9') long
HI 7855 SERIES CABLE CONNECTORS
CONNECTOR AND 3.0 mm (0.12") CABLE WITH BNC
CONNECT TO THE
BNC SOCKET
OF THE METER
CONNECT TO
SCREW TYPE
ELECTRODES
OTHER ACCESSORIES
HI 710005
HI 710006
HI 710012
HI 710013
HI 710014
HI 76405
Voltage adapter from 115 VAC to 12 VDC (USA plug)
Voltage adapter from 230 VAC to 12 VDC (European plug)
Voltage adapter from 240 VAC to 12 VDC (UK plug)
Voltage adapter from 230 VAC to 12 VDC (South Africa plug)
Voltage adapter from 230 VAC to 12 VDC (Australia plug)
Electrode holder
HI 8427
pH and ORP electrode simulator with 1 m (3.3') coaxial cable
ending in female BNC connectors
HI 931001 pH and ORP electrode simulator with LCD and 1 m (3.3')
coaxial cable ending in female BNC connectors
HI 7669/2W Temperature probe with 1 m (3.3') cable
HI 92000 Windows® compatible software
HI 920010 9 to 9-pin RS232 cable
38
RECOMMENDATIONS FOR USERS
Before using this product, make sure that it is entirely suitable for the
environment in which it is used.
Operation of this instrument in residential areas could cause unacceptable
interferences to radio and TV equipment, requiring the operator to follow all
necessary steps to correct interferences.
The glass bulb at the end of the pH electrode is sensitive to electrostatic
discharges. Avoid touching this glass bulb at all times.
During operation, ESD wrist straps should be worn to avoid possible damage
to the electrode by electrostatic discharges.
Any variation introduced by the user to the supplied equipment may degrade
the instrument’s EMC performance.
To avoid electrical shock, do not use this instrument when voltages at the
measurement surface exceed 24 VAC or 60 VDC.
To avoid damage or burns, do not perform any measurement in microwave
ovens.
39
MAN254
10/04
40
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