Explorer™

Biotechnology
Biotechnology
C A P T I VAT I N G
C A P T I VAT I N G
BIOTECHNOLOGY EXPLORER™
Explorer
S C I E N C E E D U C AT I O N
™
C A P T I VAT I N G
SCIENCE
E D U C AT I O N
Can worms really learn? Find out for yourself with our
new C. elegans Behavior Kit (see. p. 16). That’s just one
of the fun things we develop as a Biotechnology Explorer
R&D scientist. We pride ourselves on the quality of our educational products, which are designed and
manufactured to the exceptionally high standards relied on by our industry research customers. Every kit,
from concept to end product used in the classroom, is developed in conjunction with educators and with
students in mind to provide the best and most comprehensive hands on learning experience possible.
Jose Lopez, PhD / Biotechnology Explorer Program scientist
Bio-Rad
Laboratories, Inc.
Bulletin 2112 Rev J
G
13-1519
0214
Sig 1213
2014/15
Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 11 3065 7550
Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00
France 01 47 95 69 65 Germany 089 31 884 0 Greece 30 210 9532 220 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300
Israel 03 963 6050 Italy 39 02 216091 Japan 81 3 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 0318 540666
New Zealand 64 9 415 2280 Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04
Singapore 65 6415 3188 South Africa 27 861 246 723 Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland 026 674 55 05
Taiwan 886 2 2578 7189 Thailand 1800 88 22 88 United Kingdom 020 8328 2000
Life Science
Group
Explorer
S C I E N C E E D U C AT I O N
™
Biotechnology Explorer™ Program
It takes a village! As if shrinking budgets and the
fast pace of scientific discovery weren’t enough, add in
the Next Generation Science Standards, Advanced
Placement redesign, and the economic imperative
to develop a pipeline of students ready for STEM
careers. The good news is, all of these changes
point to students developing the skills
needed to compete in the 21st century.
As a company that develops and
manufactures materials and equipment
for the life science industry, research
methods and engineering practices
are part of our DNA. We focus on
providing teachers with the best
resources possible so you
can focus on what you
do best — teach!
5>
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On the cover: DNA is in every
living thing and every fish. But
finding out that your hamachi
sushi is really a habachi at your
local bento bar is a no no. Use
the new Fish DNA Barcoding kit
to find out for yourself. See page
60 for more information on this
exciting new kit and see how
much fun your class can have
being citizen scientists.
1-800-4BIORAD (1-800-424-6723)
1>
2>
3>
1. Tracy High School, Tracy, California; 2. NABT, Chicago, Illinois; 3. Solano Community College, Fairfield,
California; 4. Genes in a Bottle Kit Fundraiser, Lawndale, California; 5. Bio-Rad Teacher Workshop, Caracas,
Venezuela; 6. North Carolina School of the Arts, Winston-Salem, North Carolina.
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1
New Resources, Kits, and Products
Home Science Kits, pp. 10–13
C. elegans Behavior Kit,
pp. 16–17
Rapid Blotting — V3
Western Workflow™, pp. 42–43
New and Improved Genes in a Bottle Kit
with helix screwcap keepsakes,
pp. 46–47
IDEA and STEM
Electrophoresis Kits,
pp. 48–49
Fish DNA Barcoding Kit, pp. 60–61
DNA Model Kit, p. 117
UView™ 6x loading dye, p. 109
UView Mini Transilluminator, p. 132
8-Channel Professional
Micropipet, p. 138
2
Biotechnology Explorer Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Complete Teaching Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Explorer Community and Online Resources . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Life Science Core Content . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Home Science Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Genes in a Bottle Home Science Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
DNA Model Home Science Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
The Candy Caper: A Crime Scene Detective Kit . . . . . . . . . . . . . . . . . . . . . . . . . .13
pGLO™, GFP, Microbiology, and Model Organism Kits . . . . . . . . . . . . . . . . . . 14
C. elegans Behavior Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
pGLO Bacterial Transformation Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Green Fluorescent Protein Chromatography Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 20
pGLO Kit SDS-PAGE Extension . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Secrets of the Rainforest™ Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Microbes and Health Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Protein Analysis Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Biofuel Enzyme Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Size Exclusion Chromatography Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32
Got Protein?™ Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
ELISA Immuno Explorer™ Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Comparative Proteomics Kit I: Protein Profiler Module . . . . . . . . . . . . . . . . . . . . . 38
Comparative Proteomics Kit II: Western Blot Module . . . . . . . . . . . . . . . . . . . . . . 40
Rapid Blotting — V3 Western Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
DNA Analysis and Agarose Gel Electrophoresis Kits . . . . . . . . . . . . . . . . . . 44
Genes in a Bottle™ Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
IDEA and STEM Electrophoresis Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Forensic DNA Fingerprinting Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Lambda DNA Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
PCR Amplification Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Crime Scene Investigator PCR Basics™ Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
PV92 PCR Informatics Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Fish DNA Barcoding Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
GMO Investigator™ Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Real-Time PCR Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Cloning and Sequencing Explorer Series . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Protein Expression and Purification Series . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Refresh Kit Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Biotechnology: A Laboratory Skills Course — Textbook . . . . . . . . . . . . . . . 86
Textbook Equipment and Kit Bundles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Instructional Laboratory Equipment Packages . . . . . . . . . . . . . . . . . . . . . . . 92
DNA and Protein Electrophoresis Lab Packages . . . . . . . . . . . . . . . . . . . . . . . . . 94
Biotech Starter and PCR Instructional Lab Packages . . . . . . . . . . . . . . . . . . . . . 96
Complete Biotechnology Instructional Lab Package . . . . . . . . . . . . . . . . . . . . . . 98
Advanced Biotechnology Lab Package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
DNA and Genomic Studies: Equipment and Reagents . . . . . . . . . . . . . . . . 102
Horizontal DNA Electrophoresis Gel Boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
DNA Electrophoresis Reagent Packs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
Agarose Gel Drying Film and Agarose Powders . . . . . . . . . . . . . . . . . . . . . . . . . 106
DNA Electrophoresis Buffers and Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Precast Agarose Gels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
DNA Electrophoresis Size Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
DNA Stains . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
PCR Thermal Cyclers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
PCR Plastic Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Real-Time PCR Detection Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Amplification/PCR Reagents and Gene Transfer . . . . . . . . . . . . . . . . . . . . . . . . 114
Cell Counting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Nucleic Acid Purification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
DNA Model Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Proteins and Proteomic Studies: Equipment and Reagents . . . . . . . . . . . 118
Vertical Protein Electrophoresis Gel Boxes and Gel Drying System . . . . . . . . . . 120
Western Blotting Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Precast Polyacrylamide Gels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
Protein Electrophoresis Buffers and Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Protein Electrophoresis Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126
Protein Stains . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
Molecular Biology Laboratory: Equipment and Supplies . . . . . . . . . . . . . . 128
Electrophoresis Power Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130
Spectrophotometer and Cuvettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
Gel Imaging and Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132
UV Illumination, Microplate Reader and Software . . . . . . . . . . . . . . . . . . . . . . . . 133
Centrifuges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
Mixing Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
Temperature Control Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136
Micropipets, Micropipet Racks, and Pipet Controller . . . . . . . . . . . . . . . . . . . . . 138
Pipet Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140
Chromatography Media and Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141
Low-Pressure Protein Purification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141
Racks and Storage Boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142
Microcentrifuge Tubes and PCR Plastic Supplies . . . . . . . . . . . . . . . . . . . . . . . . 143
Plastic Refresh Kit Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143
Ordering and General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
Educational Discount Policy, Ordering Information, and Technical Support . . . . 144
Prices and Terms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Customer Services and Educational Pricing Contract . . . . . . . . . . . . . . . . . . . . 146
Subject Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148
Catalog Number Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152
TABLE OF CONTENTS
Explorer Community, pp. 6–7
Table of Contents
3
Complete Teaching Support
BIOTECHNOLOGY EXPLORER PROGRAM: COMPLETE TEACHING SUPPORT
Teacher
Supplement
Laboratory
Notebook
Curricula
The Biotechnology Explorer kit curriculum manuals have long set
the standard for in-depth teacher background and preparation
information with easy-to-implement student lab protocols and
inquiry guides. With the lab textbook, Biotechnology: A Laboratory
Skills Course, educators are provided with a comprehensive solution
for implementing core labs using a skills-based approach.
Biotechnology may seem like a science with a very specialized set
of techniques and concepts. The reality, however, is that molecular
biology and biotechnology skills and concepts are now integrated
into all life science disciplines. Biotechnology actually provides a
toolkit for exploration in multiple subject areas from basic biology
to ecology and biomedical sciences to agriculture. Several
organizations have developed curricula based on Bio-Rad’s robust
kits and textbook. Visit explorer.bio-rad.com for more information
regarding these specialized programs, including:
• The University of North Carolina at Chapel Hill’s DESTINY
Traveling Science Learning Program
• Curriculum for Agricultural Science Education (CASE)
• Project Lead The Way Biomedical Sciences
Teacher Professional Development
We can help you! Keep current with the latest life science advances
and discoveries, align with education standards, and maximize
student achievement. Bio-Rad’s curriculum training specialists work
with educators to help provide engaging and inquiry-based life
science education in ways that align with curriculum and skills
development goals. Join us at national, regional, and local science
education conferences for hands-on experience, or contact us at
[email protected] for on-site professional
development — designed to fit your needs and surpass your
expectations.
Biotechnology Explorer Kit Curricula and Instruction
Manuals are available free on the Web. The Cloning and
Sequencing Explorer Series curriculum resource CD is available
at an additional cost.
4
1-800-4BIORAD (1-800-424-6723)
Textbook
Technique
Videos and
Presentations
Biotechnology: A Laboratory Skills Course textbook,
laboratory notebook, and teacher supplement. See page 86 for
content and ordering information.
Teaching Resources
Looking for classroom-ready resources and materials to prepare
you for new techniques and content? Check out our new Explorer
Community online to access a host of free resources, including:
• Technique videos
• Curriculum manuals
• Standards alignment
• PowerPoint presentations
• Webinars
• Educator-submitted lesson plans, extensions, and inquiry-based
teaching approaches
Posters
Application Notes and More
Partnerships
Collaboration with other education suppliers and organizations that
share our vision of providing the best experiences for science
education enriches the options and support for all. Please visit the
following partners who exemplify our mission:
• Vernier Software & Technology — experts in data collection and
analysis, www.vernier.com/biotech
• Bio-Link — National Advanced Technological Education (ATE)
Center of Excellence for Biotechnology and Life Sciences,
www.bio-link.org
• Science Buddies — encouraging budding young scientists
through science fairs, www.sciencebuddies.org
Student Kit Manuals
Kit Curricula
Visit us on the Web at explorer.bio-rad.com
5
Explorer Community and Online Resources
Check out our new Explorer Community
Additional Curricula
The University of North Carolina at Chapel Hill’s DESTINY
Traveling Science Learning Program has made its learning
curriculum more widely available at the national level through a
partnership with Bio-Rad Laboratories. Visit us at explorer.bio-rad.com
to download full versions of DESTINY’s curriculum modules.
Browse the product
pages for detailed
information on
classroom kits,
equipment, and
supplies
Download free
curriculum from
the product page
(user name and
password log-in
required)
Share your stories,
educational materials,
and science artwork
and see what other
educators are doing
Learn more
See which kits fit
within your course
or subject
Learn more
BIOTECHNOLOGY EXPLORER PROGRAM: ONLINE RESOURCES
Online Resources
Bio-Rad’s website makes it easier than ever to find the product
information and online resources you’ve come to rely on. Tabs,
overlays, and other new features contain easy-to-access content.
Within a product page you can order products and related items,
add items to hot lists, request quotes, order and download
documentation, and access a wide variety of in-depth information,
all without leaving the page.
Discover and use all the classroom
resources available including
PowerPoint presentations, animations,
videos, funding resources, professional
development opportunities, and more
New: See discounted
EDU pricing — no log
in required!
Request a
price quote
Take advantage of our support services and access multiple
support resources right from your computer!
Tutorials — for frequently asked questions on key applications
View product overlays, videos, kit workflows,
curriculum fit, PowerPoint presentations, and more!
6
1-800-4BIORAD (1-800-424-6723)
Log a tech support case — create new or update existing cases
Workshop schedule — attend a workshop in your area
(explorer.bio-rad.com/workshops)
Visit us on the Web at explorer.bio-rad.com
7
Life Science Core Content
epidemiology
and global
challenges
Cladograms and
phylogenetic
relationships
Proteomics
Mechanisms
of evolution
Evolution
Genetic
engineering
Cloning
Science and
Engineering
Practices
Life
Science
Core
Content
Structure
and function
of DNA
Molecules
to Organisms
Evaluation of
hypothesis
Chemical
properties
of biological
molecules
Coupled
reactions and
free energy
changes
Central dogma:
DNA > RNA >
Protein > Trait
Physiology,
immunology,
and virology
Buffers,
solubility,
and pH
Biochemistry
and molecular
interactions
Gel
electrophoresis
Enzymesubstrate
interactions
Chromatography
Mendelian
genetics and
inheritance
patterns
Gene
regulation
Mutation
PCR
Independent
student research
projects
Bioinformatics
Genetics
and Heredity
Biochemistry
Drug
discovery
1-800-4BIORAD (1-800-424-6723)
Use of
sophisticated
laboratory
techniques
Cellular
Processes
Hardy-Weinberg
principle
Western
blotting
8
Interpretation
of experimental
results
Ecosystems,
Environment,
and
Interactions
Physical
environment
as a selective
force
Natural
selection
Use of
experimental
controls
possibilities of
science and
technology
Interdependence
of organisms
BIOTECHNOLOGY EXPLORER PROGRAM: LIFE SCIENCE CORE CONTENT
Create context. Reinforce learning.
Genetically
Biodiversity
modified
Stay current. New scientific discoveries and
organisms
technologies create more content for you to
teach, but not more time. Biotechnology
Explorer kits help you teach more
efficiently by integrating multiple core
content subjects into a single lab, and
Populations,
Genetic
ecosystems,
our modular series create ready-to-go
ELISA
testing
and human
multiweek courses so that you
impacts
can focus on what matters
most — teaching. Connect
concepts with techniques
Role, place,
and put them into context
Environmental
limits, and
with real-world scenarios.
Diseases and
changes
Genes, alleles,
chromosomes,
and genomes
DNA structure,
function, and
chemistry
Genomics
and
proteomics
DNA
fingerprinting
Bacterial
transformation
Visit us on the Web at explorer.bio-rad.com
9
Home Science Kits: Real Science, Real Fun!
Genes in a Bottle™ Home Science Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
DNA Model Home Science Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
The Candy Caper, A Crime Scene Detective Kit . . . . . . . . . . . . . . . . . . . . . 13
“
As a writer and a parent,
I appreciate how my
childrens’ imaginations
were brought into play with
this innovative science kit!
They loved reading the
Candy Caper mystery and
then using real forensic
science to solve the case —
a winner all the way around!
3>
BIOTECHNOLOGY EXPLORER PROGRAM: HOME SCIENCE KITS
Home Science Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
”
Jennifer Lynn Alvarez
Children’s Book Author
Windsor, California
2>
1>
10
1-800-4BIORAD (1-800-424-6723)
4>
1. North Carolina School of the Arts, Winston-Salem, North Carolina; 2. Astley
Cooper School, Hemel Hempstead, Hertfordshire, United Kingdom; 3. Santa Fe
High School, Alachua, Florida; 4. Bio-Rad Teacher Workshop, Blacksburg, Virginia.
Visit us on the Web at explorer.bio-rad.com
11
New Home Science Kits: Real Science, Real Fun!
Experiments for kids — science is play
Kids are born natural scientists and love to experiment. It takes the combined efforts
of parents and educators to foster scientific curiosity and help students build scientific
intuition that could one day translate into a great scientific discovery or invention.
Bio-Rad’s easy and fun science education kits combine real-life science with play
for kids to discover and explore the biology and biotechnology world around them.
Home science kits cannot be purchased directly from Bio-Rad, but can easily be
purchased on Amazon.com, or inquire with your local toy and educational supply store.
The home science kits are available for purchase only in the U.S.
New Genes in a Bottle Home Science Kit
Make invisible DNA visible in a matter of minutes!
Already a hit in classrooms, Bio-Rad’s classroom-size Genes in a
Bottle™ kit for 36 students (p. 46) has been optimized and streamlined
for easy and fun use at home with a one-step DNA extraction buffer
(for 2 necklaces). This comprehensive and easy-to-use home science kit
enables young scientists to collect their own DNA, watch it appear before their eyes,
and then display it in a clear double helix necklace. They’ll continue adventures into
the remarkable world of what makes us all unique (DNA) by learning about the actual
double-helix structure of DNA. Using the ancient art of origami, they can color, cut,
and fold paper to make a DNA model. They can also color and explore human cells
and learn exactly where their DNA comes from. And much more!
New DNA Model Home Science Kit
Attach, assemble, and twist.
As common as DNA is, it can be a tough topic to understand. But that’s
where this colorful and attractive DNA model comes into play in your
home. Your child will immediately become engaged with this friendly,
interactive foam model of DNA that measures 2 feet tall! It’s easy, just
attach and assemble the DNA, and then twist to transform it into the familiar double
helix. Just like a puzzle — and just as in nature — this DNA model fits together only
one way: Adenine (A) fits only into Thymine (T) and Guanine (G) fits only into Cytosine
(C) on the sugar-phosphate backbones. Build a biologically correct DNA model that
can be used in a number of fun and educational ways.
Genes in a Bottle Home Science Kit
Genes in a Bottle Home Science Kit
Kit creates 2 DNA helix keepsakes (U.S. only)
Catalog # . . . . . . . . . . . . . . . . . . . . . . . . .Retail Price
166-7010 . . . . . . . . . . . . . . . . . . . . .$29.95
DNA Model Home Science Kit
The DNA model includes everything you need to build one (1) biologically correct
DNA model with an instruction sheet and 10 fun facts about DNA!
New The Candy Caper,
A Crime Scene Detective Kit
Solve a forensic mystery story using engineering and science.
Get ready to crack cases and bring criminals to justice with The
Candy Caper, a crime scene detective kit. Engage children as
they play the role of forensic scientist and solve the case of the
stolen cell phones. Candy provides the crime scene evidence as
they engineer a gel electrophoresis system to sort out the suspects and
determine “who done it.” After solving the case, they’ll discover more science
experiments to further develop their science sleuthing skills and dive deeper
into the world of chemistry and biology by creating their own pH paper and
indicator solution, separating molecules using paper chromatography, and
perform another electrophoresis experiment using purple cabbage juice.
A total of 12 activities are possible with this kit, with enough supplies
for two mystery gels. A few supplies from home are necessary
and include: M&M’S candies, 9 V batteries (5), bottled
water (1), purple cabbage, measuring cups and
spoons, salt, toothpicks, and a microwave oven.
DNA Model Home Science Kit (U.S. only)
BIOTECHNOLOGY EXPLORER PROGRAM: HOME SCIENCE KITS
Supporting science education and
literacy beyond the classroom
Millions of students worldwide have been using
Bio-Rad’s Biotechnology Explorer™ kits to
engage in hands-on science exploration in the
classroom for over 15 years. Bio-Rad is now
bringing that same level of expertise into the
home with our home science kits. These home
science kits will delight all levels of the sciencecurious, from the child who has moved beyond
space sand to the do-it-yourself (DIY) scientist looking to build his or her
skill level to the home school teacher looking to integrate hands-on learning.
Catalog # . . . . . . . . . . . . . . . . . . . . . . . . .Retail Price
166-7015 . . . . . . . . . . . . . . . . . . . . .$24.99
The Candy Caper, A Crime Scene
Detective Kit
The Candy Caper, A Crime Scene
Detective Kit (U.S. only)
Catalog # . . . . . . . . . . . . . . . . . . . . . . . . .Retail Price
166-7020 . . . . . . . . . . . . . . . . . . . . .$49.99
12
Visit us on the Web at explorer.bio-rad.com
13
Classroom Kits: pGLO™, GFP, Microbiology and Model Organism Kits
Section Contents
New C. elegans Behavior Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
pGLO™ Bacterial Transformation Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Green Fluorescent Protein Chromatography Kit . . . . . . . . . . . . . . . . . . . . . 20
pGLO Kit SDS-PAGE Extension . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Secrets of the Rainforest™ Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Microbes and Health Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
“
My students have
benefited from the inquiry
focused lab activities and
the clear directions. I have
benefited from the fact that
the labs are well thoughtout and easy to prepare.
”
1>
CLASSROOM KITS: pGLO, GFP, MICROBIOLOGY, AND MODEL ORGANISM KITS
pGLO, GFP, Microbiology, and Model Organism Kits
Steve Cobb, MS
South Kitsap High School
Port Orchard, Washington
and Olympic College
Bremerton, Washington
3>
2>
14
4>
1-800-4BIORAD (1-800-424-6723)
1. Terra Nova High School, Pacifica, California; 2. Bio-Link Teacher Workshop, Conroe,
Texas; 3. Independence High School, Thompson Station, Tennessee; 4. Bio-Rad Teacher
Workshop, Veenendaal, Netherlands.
15
New C. elegans Behavior Kit — AP Big Ideas 1, 2, 3, and 4: Pavlov’s Worm
C. elegans Behavior Kit
C. elegans was the first multicellular organism to have its complete genome sequenced.
The Nobel Prize in Physiology or Medicine was awarded to C. elegans researchers in
2002 (genetics of organ development and apoptosis), 2006 (RNA interference), and 2008
(GFP expression), emphasizing the importance of research on this model organism. This
microscopic nematode is ideal for students to learn about subculturing so that they can
observe the life cycle and different stages of development of the worms.
Lab Preparation Checklist
Please note that your kit contains
instructions on how to receive your
C. elegans in a separate shipment.
Prepare the NGM Lite agar plates before
you receive your C. elegans. Once you
receive your worms, immediately place
the bag containing the C. elegans in a
–70°C freezer. DO NOT store C. elegans
in a –20ºC freezer. If a –70°C freezer is
not available, keep the C. elegans on dry
ice until you are ready to plate onto NGM
Lite agar plates and are ready to follow
the C. elegans thaw procedure.
Each kit supports 32 students.
C. elegans Behavior Kit
Following life cycle observation, students will get the chance to understand how
C. elegans can learn to associate salt with their food by monitoring chemotaxis. When
wild-type C. elegans are fed food with salt they learn to associate their food with the
salt. When C. elegans then sense salt in their environment, they will migrate toward the
salt in search of food. A mutation affecting in the daf-18 gene (and subsequently the
AIY and ASE neurons) results in a worm that is not able to learn to associate the salt
with the food. While the mutant C. elegans are able to display chemotaxis in response
to many chemicals, their inability to associate their food to a salty environment results
in no migration toward salt in a chemotaxis assay.
Catalog #
List Price . . . . . . . . .EDU Price
166-5120EDU $218.75 . . . . . . .$175.00
The worms are provided frozen and must be
kept on dry ice (–70°C or colder). C. elegans
will be shipped separately on requested date.
Key Kit Features
• Aligns with AP Biology Big Ideas 1, 2, 3,
and 4; Lab 12
• Feature neurobiology, BLAST analysis, and
chi square statistic supplements
• Get hands-on experience with a model
eukaryotic organism
• Observe and study the life cycle of C. elegans
• Utilize microscope skills
• Learn about genetics and its effect on
behavior
• Complete student activities in two 45 minute
lab sessions
Note: The wild-type and mutant worms are shipped on dry ice and must be
kept on dry ice or at –70°C until ready to plate. Allow for preparation of NGM
Lite agar plates prior to receiving the worms. Wild-type and mutant C. elegans
will be shipped overnight at a date specified by the customer.
Check out our online calculator at bio-rad.com/celegansbehaviorkit2 to help plan
when to prepare the various stages of the lab.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Eggs
• Use of experimental controls
• Interpretation of experimental
results
• Sterile technique
• Statistical relevance
Ecosystems,
Environment,
and
Interactions
• Dauer state to survive harsh
environmental conditions
• Chemotactic response to
environment
• Associative learning behavior
• Subpopulations
Mature
Larvae
• Developmental processes
• Neurologic processes
Science and
Engineering
Practices
C. elegans
Behavior
Kit
Cellular
Processes
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
E. coli OP50-pBAD, lyophilized
Cholesterol in alcohol, 200 µl
Ampicillin, lyophilized, 30 mg
NGM Lite agar, 11 g
Assay agar, 4 g
2.5 M NaCl, 0.5 ml
C. elegans wash buffer (10x), 30 ml
Microcentrifuge tubes, 2.0 ml
Microcentrifuge tubes, 1.5 ml
Petri dishes, 60 mm
Disposable plastic transfer pipets
C. elegans Redemption instructions
C. elegans wild-type, 1 ml
(to be redeemed)
C. elegans mutant, 1 ml
(to be redeemed)
Curriculum, including teacher’s guide,
student manual, and graphic quick guide
1
1
1
1
1
1
1
60
30
60
50
1
1
• Use of model organisms
• Life cycle studies
• Eukaryotic cell structure
and organization
Lab 1
1
1
Required Accessories Not Included in Kit:
Dissection microscopes (10–40x zoom)
1–8
Adjustable micropipets, p. 138
2–20 µl (or 10 µl fixed volume)
8
100–1,000 µl
1–8
Pipet tips, p. 140
2–200 µl, BR-35
1–8
100–1,000 µl, BR-40
1–8
Microwave oven or magnetic hot plate
1
with stir bar
Microwave oven, hot plate, or autoclave
1
for preparing agar
Incubation oven, p. 137
1
Refresh Kit Components: (more info pp. 84, 143)
C. elegans Behavior Kit RT Reagent
$45.00
Refill Pack (166-5121EDU) includes
NGM lite and assay agar, wash buffer,
and salt solution
C. elegans Behavior Kit TS Reagent
45.00
Refill Pack (166-5123EDU) includes E. coli
OP50-pBAD, cholesterol, and ampicillin
C. elegans Behavior Kit Wild-Type and 60.00
Mutant Worm strains (166-5122EDU)
must be kept at –70°C or on dry
ice until ready to plate
Molecules
to Organisms
Genetics
and Heredity
CLASSROOM KITS: C. elegans Behavior Kit — AP Big Ideas 1, 2, 3, and 4
A new and captivating classical behavior lab with a twist. No more fruit flies!
Explore the fascinating life cycle of C. elegans through a chemotaxis experiment.
Compare a wild-type strain to a neurologic mutant to learn through a chemotaxis
experiment how the loss of the daf-18 gene impacts learning capacity.
NGM Lite Agar (166-5125EDU), 11 g,
for growth of wild-type and mutant
C. elegans worm strains
Lab 2
20.00
Lab 3
C. elegans neurons at www.openworm.org
• Effect of mutations
• Adaptation to environment
16
1-800-4BIORAD (1-800-424-6723)
Evolution
•
•
•
•
DNA > RNA > Protein > Trait
Structure and function of genes
Hermaphroditic reproduction
Genotypic and phenotypic
differences
Visit OpenWorm to see the entire worm
connectome — you can see every neuron
within the worm and how they all connect!
Visit us on the Web at explorer.bio-rad.com
17
pGLO™ Bacterial Transformation Kit — AP Big Idea 3: Shine a Little Light on Your Molecular
Jellyfish genes make the invisible — visible! In this lab, students transform
bacteria with a gene from the bioluminescent jellyfish Aequorea victoria. Bio-Rad’s
exclusive pGLO plasmid is constructed with the jellyfish gene that encodes green
fluorescent protein (GFP), an antibiotic-resistance gene that encodes β-lactamase
protein, and the araC gene, encoding a regulator protein that turns
the GFP gene on and off. Bacteria transformed with the pGLO
plasmid are selected by ampicillin resistance and, when
araC
induced to express GFP, the bugs glow
fluorescent green under UV light!
ori
pGLO
5,400 bp
Gene regulation. With the pGLO plasmid, students
analyze the growth of bacteria on various media and
GFP
Ampr
examine the roles that external and internal factors play
in gene regulation.
pGLO Bacterial Transformation Kit
Lab Preparation Checklist
Includes free UV pen light!
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
Each kit supports 32 students.
pGLO Bacterial Transformation Kit
Catalog #
List Price . . . . . . . . .EDU Price
166-0003EDU $117.50 . . . . . . . .$94.00
The curriculum manual is not included in the kit. It is
available online to download free of charge.
Convenient lyophilized reagents.
Ships at room temperature. Store at 4°C.
pGLO Bacterial Transformation Kit with
printed curriculum manual
166-0043EDU 125.00 . . . . . . . .100.00
Gene expression in all organisms is carefully regulated to allow
adaptation to differing conditions and to prevent wasteful production of proteins.
The bacterial genes encoding the enzymes needed to metabolize the simple sugar
arabinose are a perfect example. A promoter region upstream of these genes acts as
a molecular on/off switch that regulates their expression. The genes are activated only
when arabinose is present in the environment. Bio-Rad’s pGLO plasmid incorporates
the arabinose promoter, but the genes involved in the breakdown of arabinose have
been replaced with the jellyfish gene encoding GFP. When bacteria transformed with
the pGLO plasmid are grown in the presence of arabinose, the GFP gene switches
on, causing the bacteria to express GFP and to fluoresce brilliant green.
pGLO Transformation Lab Prep DVD
166-0540EDU
17.50 . . . . . . . . . .14.00
pGLO bacterial transformation lab preparation and
techniques instructional DVD contains everything you
need to know to set up and run the lab like a pro.
Key Kit Features
• Aligns with AP Biology Big Idea 3; Lab 8
• Transform bacteria with jellyfish gene
• Turn the modified genes on or off
• Study gene regulation
• Complete in two 45 minute lab sessions
When students genetically engineer bacteria with the genes from a bioluminescent
jellyfish, they never forget the central mantra of molecular biology:
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
DNA ➤ RNA ➤ Protein ➤ Trait — Green Fluorescence!
•
•
•
•
Ecosystems,
Environment,
and
Interactions
• Genetically modified
organisms (GMOs)
• GMOs in research, medicine,
nutrition, and bioremediation
• Global challenges of GMOs
• Microbiology
Science and
Engineering
Practices
pGLO
Bacterial
Transformation
• Antibiotic selection and
resistance genes
• Selection mechanisms
• Adaptation to environment
• Bacterial conjugation and
gene transmission
18
1-800-4BIORAD (1-800-424-6723)
Cellular
Processes
Evolution
Plasmid (pGLO), lyophilized
1
E. coli strain HB101 K-12, lyophilized
1
LB nutrient broth, sterile
1
LB nutrient agar powder
1
Ampicillin, lyophilized
1
Arabinose, lyophilized
1
1
Sterile transformation solution (CaCl2)
Petri dishes, 60 mm, sterile
40
Inoculation loops, sterile
80
Microcentrifuge tubes, 2.0 ml, sterile,
60
color coded
Foam floats
8
Disposable plastic transfer pipets
50
UV pen light
1
We’ve gone green! The curriculum, including
teacher’s guide, student manual, and
graphic quick guide, is available to
download free online or for a small fee
when ordering with the kit (#166-0043EDU).
Recommended (Optional) Accessories:
Incubation oven, p. 137
Water bath, p. 137
UV lamps, p. 133
4–8
Microwave oven
Refresh Kit Components: (more info pp. 84, 143)
Transformation kit reagent refill pack
$59.00
(#166-0555EDU) includes pGLO
plasmid, E. coli strain HB101 K-12,
LB nutrient broth, transformation
solution, ampicillin, arabinose
pGLO plasmid (#166-0405EDU)
E. coli strain HB101 K-12
(#166-0408EDU)
LB nutrient broth, 10 ml
(#166-0421EDU)
Ampicillin (#166-0407EDU)
Arabinose (#166-0406EDU)
LB nutrient agar powder,
20 g (#166-0600EDU) or
500 g (#166-0472EDU)
Transformation solution, 15 ml
(#166-0409EDU)
Petri dishes, 60 mm, sterile, 500
(#166-0470EDU)
Inoculation loops, 10 µl, sterile, 80
(#166-0471EDU)
Disposable plastic transfer pipets,
sterile, 500 (#166-0474EDU)
Jellyfish foam floating racks, 8 racks
(#166-0479EDU)
Curriculum manual, printed
(#166-0033EDU)
33.00
18.00
17.00
17.00
28.00
12.00
89.00
20.00
128.00
13.00
105.00
Lab 1
30.00
6.00
Molecules
to Organisms
Kit
• Prokaryotic cell structure
and cell division
• Selective growth media
• Bacterial metabolism
Genetic engineering of organisms
Use of experimental controls
Interpretation of experimental results
Calculate transformation efficiency
CLASSROOM KITS: pGLO BACTERIAL TRANSFORMATION KIT — AP BIG IDEA 3
Genetic engineering is the process of manipulating the genetic material of an
organism, often to include the DNA from a foreign organism. In this activity, students
transform bacteria by introducing a gene from a bioluminescent jellyfish. They use the
same procedure used for creating “designer proteins” that has led to the explosion of
new health treatments, agricultural applications, and environmental solutions.
Biology — Literally!
Genetics and
Heredity
•
•
•
•
• DNA structure, function,
and chemistry
• Chemical properties of
biological molecules
• Effects of temperature and
pH on biochemical reactions
DNA > RNA > Protein > Trait
Bacterial transformation
The lac operon
Creating genetically engineered
organisms (GMOs)
• Structure and function of genes
• Gene regulation and transcription
factors
Lab 2
Extra Curriculum for pGLO on the Web
Download the BioBusiness curriculum that
embeds the pGLO transformation lab in topical
case study scenario format, developed by the
University of North Carolina, Chapel Hill DESTINY
Traveling Science Learning Program. Download
the complete PDF free at explorer.bio-rad.com.
Visit us on the Web at explorer.bio-rad.com
19
Green Fluorescent Protein (GFP) Chromatography Kit: Capture the Glow!
GFP Chromatography Kit*
Each kit supports 32 students.
Green Fluorescent Protein
Chromatography Kit*
Chromatography 101. Chromatography to purify proteins of interest depends on a
protein’s chemical or physical properties, such as molecular weight, electrical charge, or
solubility. Green fluorescent protein (GFP) is extremely hydrophobic compared to most
bacterial proteins. This unique characteristic of GFP enables the purification of GFP
from bacterial cell proteins using hydrophobic interaction chromatography (HIC). When
placed in a buffer containing a high concentration of salt, the HIC matrix selectively binds
hydrophobic GFP molecules while allowing the bacterial proteins to pass right through
the column. Then, simply lowering the salt concentration of the buffer causes GFP to
elute from the column in a purer form.
Catalog #
List Price . . . . . . . . .EDU Price
166-0005EDU $128.75 . . . . . . .$103.00
Ships at room temperature.
Store lysozyme at –20°C.
* Transformed bacterial colonies from pGLO
bacterial transformation kit required. See page 18.
Key Kit Features
• Transform bacteria with a gene from a
bioluminescent jellyfish (AP Big Idea 3)
• Induce transformed bacteria to overexpress
green fluorescent protein
• Purify GFP using chromatography
• Complete in three 45 minute lab sessions
Students begin this activity with the pGLO bacterial transformation kit. A colony of
transformed bacteria is placed in liquid culture overnight, then lysed to release their
cellular contents. GFP is purified from the bacterial contaminants using the HIC columns
provided in the kit.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
The unique fluorescent property of GFP allows real-time monitoring of the extraction
and purification processes. pGLO transformation and GFP purification together are key
processes used in biotechnology to produce and purify designer proteins with commercial
or research value.
Ecosystems,
Environment,
and
Interactions
• Antibiotic resistance
genes
• Selection mechanisms
• Adaptation to
environment
20
1-800-4BIORAD (1-800-424-6723)
Science and
Engineering
Practices
GFP
Chromatography
Kit
Cellular
Processes
Evolution
Ampicillin, lyophilized
Arabinose, lyophilized
Lysozyme, lyophilized
LB nutrient broth capsule
Binding buffer
Column equilibration buffer
Column wash buffer
Elution buffer
HIC chromatography columns
Inoculation loops, sterile
Disposable plastic transfer pipets
Microcentrifuge tubes, 2.0 ml, clear
Cell culture tubes, 15 ml, sterile
Sample collection tubes, 5 ml
Curriculum, including teacher’s guide,
student manual, and graphic quick guide
1
1
1
1
1
1
1
1
8
20
40
30
25
25
1
Lab 1
Required Accessories Not Included in Kit:
Transformed bacterial colonies from
pGLO bacterial transformation kit, p. 18
UV lamps, p. 133
4–8
Centrifuges, p. 134
1–2
Recommended (Optional) Accessories:
Incubation oven, p. 137
Rocking platform, pp. 135–136
Tube roller, p. 135
Microwave oven
Lab 2
Refresh Kit Components: (more info pp. 84, 143)
GFP lyophilized components refill pack $69.00
(#166-0015EDU) includes ampicillin,
arabinose, LB nutrient broth tablet,
lysozyme
GFP chromatography buffers refill pack 57.00
(#166-0016EDU) includes binding
buffer, column equilibration buffer,
column wash buffer, elution buffer
• Use of chromatography
to purify a protein
• Interpretation of
experimental results
• Evaluation of hypotheses
• Prokaryotic cell structure
and division
• Bacterial metabolism
• Selective growth media
Lab Preparation Checklist
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
In biotechnology research and industry, transformation is the first step in producing
genetically engineered “designer” proteins. Genetically engineered proteins have
unlimited applications — from medicines to treat human diseases to powerful enzymes
incorporated into nonpolluting laundry detergents — but they must be overexpressed,
mass produced, and purified to be marketable.
• Pharmaceutical drug
discovery
• Genetically modified
organisms (GMOs)
• GMOs in research, medicine,
nutrition, and bioremediation
• Microbiology
CLASSROOM KITS: GREEN FLUORESCENT PROTEIN (GFP) CHROMATOGRAPHY KIT
Take your pGLO™ transformation lab to the next level! Take your students
through the process of creating a new product — from lab discovery to biomanufacturing
to market. Show your students the relevance of the science they learn in the classroom
to science in their lives. Encourage them to imagine the possibilities and to think critically
and creatively.
Molecules
to Organisms
Genetics and
Heredity
• Protein structure (1°, 2°, 3°, 4°)
• Enzymes and catalysis
• Chemical properties of
biological molecules
• Hydrophobicity of proteins
• Chromatographic separation
of biomolecules
• DNA > RNA > Protein > Trait
• Gene regulation and
transcription factors
HIC chromatography columns and
41.00
caps, 8 each (#166-0413EDU)
Inoculation loops, 10 µl, sterile, 80
13.00
(#166-0471EDU)
Jellyfish foam floating racks, 8 racks
30.00
(#166-0479EDU)
Disposable plastic transfer pipets,
105.00
sterile, 500 (#166-0474EDU)
Cell culture tubes, 17 x 100 mm, 14 ml, 17.00
sterile, 25 (#166-0476EDU)
Clear polystyrene tubes, 13 x 100 mm, 39.20
9 ml, 1,000 (#223-9750EDU)
Green racks, set of 5 racks
50.01
(#166-0481EDU)
15 ml tube racks, holds 60 tubes,
72.00
set of 5 racks (#166-0483EDU)
67.20
Poly-Prep® columns, empty, 50
(#731-1550EDU)
295.20
Macro-Prep® HIC Support, 100 ml
(#156-0080EDU)
Lab 3
Extra Curriculum for GFP on the Web
Download the Weigh To Go! curriculum that
embeds the GFP purification lab in topical case
study scenario format, developed by the
University of North Carolina, Chapel Hill DESTINY
Traveling Science Learning Program. Download
the complete PDF free at explorer.bio-rad.com.
Visit us on the Web at explorer.bio-rad.com
21
pGLO™ Kit SDS-PAGE Extension: What’s after pGLO Bacterial Transformation?
pGLO Kit SDS-PAGE Extension
DNA ➤ RNA ➤ Protein ➤ Trait — Green Fluorescence!
Each kit supports 32 students.
The two Biotechnology Explorer kits used in this application, pGLO bacterial
transformation kit (#166-0003EDU) and pGLO kit SDS-PAGE extension (#166-0013EDU)
can be used to directly link gene expression to identification of a protein responsible for
a specific trait. In the first part of the exercise, a plasmid encoding GFP is transformed
into E. coli, a common prokaryotic organism used for DNA propagation and protein
expression. Colonies of E. coli are qualitatively examined for fluorescence, which suggests
that the pGLO gene is being expressed. In the second part of the lab, gel electrophoresis
is used to separate the entire repertoire of proteins expressed in E. coli, which includes
the foreign GFP responsible for the fluorescence trait.
™
pGLO Kit SDS-PAGE Extension
Catalog #
List Price . . . . . . . . .EDU Price
166-0013EDU $118.67 . . . . . . . .$89.00
Ships at room temperature.
Store Precision Plus Protein™ Kaleidoscope™
standards at –20°C. Mini-PROTEAN® TGX™ precast
gels (12 month shelf life) available separately.
Key Kit Features
• Perform real biotechnology workflows
• Identify proteins by conformation and size
• Link gene induction to protein expression
to protein identification
• Understand chromophores and the basis
of protein fluorescence
This extension links two of the most commonly used techniques in biotechnology labs:
transformation and electrophoresis. Moreover, this extension illustrates the versatility and
robustness of one of the most commonly used proteins in modern biology, GFP. In its
native environment, GFP fluoresces in the deep sea jellyfish, Aequorea victoria. GFP
retains its fluorescent properties when cloned and expressed in E. coli and when
isolated from E. coli and separated on polyacrylamide gels. These amazing properties
of GFP and the powerful methodologies of protein electrophoresis allow students
to visualize the phenotypic properties of a protein and identify the single protein “band”
responsible for the trait. Find the protein in the haystack!
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
• Genetic engineering of
organisms
• Use of experimental controls
• Interpretation of experimental
results
Ecosystems,
Environment,
and
Interactions
Science and
Engineering
Practices
pGLO Kit
SDS-PAGE
Molecules
to Organisms
Extension
• Prokaryotic cell structure
and cell division
• GFP structure
• Antibiotic selection and
resistance genes
• Selection mechanisms
• Adaptation to environment
22
1-800-4BIORAD (1-800-424-6723)
Cellular
Processes
Evolution
Lab Preparation Checklist
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
Take white and green colonies from your transformed plates, prepare sample lysates,
and identify the pGLO protein on SDS-PAGE gels.
• Genetically modified
organisms (GMOs)
• GMOs in research, medicine,
nutrition, and bioremediation
CLASSROOM KITS: PGLO KIT SDS-PAGE EXTENSION
Don’t stop at cloning the gene — identify the protein responsible for green
fluorescence! The bacterial proteome contains thousands of proteins, but only the
cloned green fluorescent protein (GFP) glows! Learn how GFP expressed from Bio-Rad’s
pGLO plasmid can be used to help illustrate and teach the central doctrine of biology,
from the transformation of DNA to the expression of a protein to the visualization of a trait.
Genetics and
Heredity
• Chemical and physical
properties of proteins
• Protein structure
(1°, 2°, 3°, 4°) and function
• Protein extraction techniques
• Chemistry of protein
electrophoresis
Dithiothreitol (DTT), 1 g
Precision Plus Protein Kaleidoscope
standards, 50 µl
Bio-Safe™ Coomassie stain for proteins,
100 ml
10x Tris/glycine/SDS electrophoresis
buffer, 1 L
Laemmli sample buffer, 30 ml
Curriculum, including teacher’s guide
and student manual
1
2
1
1
1
1
1
Required Accessories Not Included in Kit:
Amp (white) and amp/ara (green)
4–8
colonies from plates from the pGLO
bacterial transformation lab
Mini-PROTEAN TGX precast
4–8
polyacrylamide gels, Any kD™, 10-well
each, p. 123
Vertical gel electrophoresis chambers,
1–4
p. 120
Power supplies, p. 130
1–4
Water bath or dry bath, pp. 136–137
1
Adjustable micropipets, p. 138
2–20 µl
4–8
100–1,000 µl
4–8
Pipet tips, p. 140
2–200 µl, BR-35
1 bag
100–1,000 µl, BR-40
1 bag
0.5–200 µl, Prot/Elec™
1 bag
UV lamps, p.133
4–8
Screwcap microcentrifuge tubes,
1 bag
1.5 ml, 500, p. 143
Gel staining trays, p. 143
4–8
Foam floating racks, p. 143
8
Recommended (Optional) Accessories
GelAir™ drying system, p. 121
GelAir assembly table, p. 121
GelAir drying frames with clamps, p. 121
GelAir cellophane, p. 121
Rocking platform, pp.135–136
Gel documentation system, p. 132
Lab 1
Refresh Kit Components: (more info pp. 84, 143)
Gel staining trays, 4
$16.00
(#166-0477EDU)
Inoculation loops, 10 µl, sterile, 80
13.00
(#166-0471EDU)
Jellyfish foam floating racks, 8 racks
30.00
(#166-0479EDU)
Green racks, set of 5 racks
50.01
(#166-0481EDU)
DTT, p. 125
Precision Plus Protein Kaleidoscope
standards, p. 126
Bio-Safe Coomassie stain, p. 127
10x Tris/Glycine/SDS electrophoresis
buffer, p. 125
Laemmli sample buffer, p. 125
• DNA > RNA > Protein > Trait
• Creating genetically engineered
organisms (GMOs)
• Structure and function of proteins
Visit us on the Web at explorer.bio-rad.com
23
Secrets of the Rainforest™ Kit: Apply Genetic Engineering to Cure Human Diseases
Secrets of the Rainforest Kit
Module 1: Preparing a Bacterial Library
Bacterial library, E. coli, lyophilized
1
LB nutrient agar tablets
5
Petri dishes, 60 mm, sterile
20
Inoculation loops, sterile
10
Disposable plastic transfer pipets
10
Each kit supports 32 students.
Secrets of the Rainforest Kit
How are a bacterial library created and a gene cloned? A bacterial library is constructed
by chopping up the genome of the medicinal plant (the “parent”) with a restriction enzyme. This
generates a random assortment of DNA fragments from the medicinal plant, some containing the
gene that encodes the green glowing protein with anticancer properties.
Catalog #
List Price . . . . . . . . .EDU Price
166-0006EDU
$208.75 . . . . . . . .$167.00
Ships at room temperature.
Store lysozyme at –20°C.
When the same restriction enzyme is used to cut (digest) a plasmid (the “destination”) it
produces an opening in the plasmid. The plant DNA fragments are inserted into the digested
plasmids, resulting in plasmids that contain random assortments of DNA fragments derived
from the genome of the medicinal plant. The plasmids are transformed into the bacteria E. coli,
which replicate the plasmid when they divide. Some of the transformed bacterial cells will
contain copies of the plasmid containing the plant gene that encodes the protein of interest.
Key Kit Features
• Grow a bacterial library
• Clone a gene of interest in bacteria
• Express a protein of interest in bacteria
• Purify a protein of interest from bacteria
• Take a protein to market
• Complete in four 45 minute lab sessions
The hands-on portion of the exercise has students streak out the bacterial library, grow the
bacteria, and select the colonies that have acquired the green glowing trait from a background
of nonglowing bacteria. A culture of these bacteria is grown to scale up production of the
green glowing protein, which is then purified by column chromatography. The actual source
of this fluorescent protein is the bioluminescent jellyfish Aequorea victoria. In the classroom
simulation and in commercial biotech labs the principle is the same: a gene derived from a
natural source encodes a novel protein with commercial value. This practical activity is followed
by dry-lab lessons in which the purified green protein is put through the drug discovery
process. This includes learning about Food and Drug Administration regulations, animal
testing, marketing practices for introducing a new drug, financing, ethical issues, and student
presentations. The students examine the needs and viewpoints of advocacy groups, the
biotechnology industry, and the FDA. They consider ethical, economic, social, and practical
issues and develop possible strategies for what they feel are the best outcomes.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
• Biodiversity
• Producing medicinal
plant proteins in bacteria
for human treatment
• Use of genetic engineering to
produce synthetic proteins
• Cloning genes and screening
bacterial libraries
• Interpretation of experimental
results
24
1-800-4BIORAD (1-800-424-6723)
Science and
Engineering
Practices
Secrets
of the
Rainforest
Kit
Cellular
Processes
Evolution
Module 2: Protein Purification
Ampicillin, lyophilized
Arabinose, lyophilized
Lysozyme, lyophilized
LB nutrient broth capsule
Binding buffer
Column equilibration buffer
Column wash buffer
Elution buffer
HIC chromatography columns
Inoculation loops, sterile
Disposable plastic transfer pipets
Microcentrifuge tubes, 2.0 ml, clear
Cell culture tubes, 15 ml, sterile
Sample collection tubes, 5 ml
Curriculum, including teacher’s guide,
student manual, and graphic quick guide
1
1
1
1
1
1
1
1
8
20
40
30
25
25
1
Lab 1
Lab 2
Required Accessories Not Included in Kit:
UV lamps, p. 133
4–8
Centrifuges, p. 134
1–2
Recommended (Optional) Accessories:
Incubation oven, p. 137
Rocking platform, pp. 135–136
Tube roller, p. 135
Microwave oven
Lab 3
Refresh Kit Components: (more info pp. 84, 143)
Bacterial library (#166-0411EDU),
$25.00
E. coli, lyophilized
Ecosystems,
Environment,
and
Interactions
• Prokaryotic vs.
plant cell structure
• Bacterial growth
and cell division
• Plant physiology and
medicinal properties
Lab Preparation Checklist
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
In this kit, students assume the role of scientists working for a biotechnology company,
Biotex, Inc. Biotex scientists have constructed a bacterial library from the genome of a
medicinal rain forest plant whose green glowing leaves have anticancer properties. Now
the particular protein must be identified and a gene cloned. The kit applies the principles
of DNA restriction analysis, bacterial transformation, and protein purification and guides
students through the process of drug discovery. Then the students simulate the process
of taking a cloned protein to market as a treatment for cancer.
• Pharmaceutical drug
discovery
• Human physiology and cancer
• GMOs in medicine
• Antibiotic resistance
CLASSROOM KITS: SECRETS OF THE RAINFOREST KIT
The Secrets of the Rainforest lab is a fantastic way to introduce your students to the
world of commercial biotechnology by involving them in a simulated scenario of a company
in the business of drug discovery.
Molecules
to Organisms
Genetics and
Heredity
•
•
•
•
• Chemical and physical
properties of proteins
• Protein structure (1°, 2°, 3°, 4°)
• Chromatographic separation
of biomolecules
DNA > RNA > Protein > Trait
Expression of genes in foreign hosts
Genetic engineering to create GMOs
Gene regulation mechanisms
GFP lyophilized components refill pack
(#166-0015EDU) includes ampicillin,
arabinose, LB nutrient broth tablet,
lysozyme
69.00
GFP chromatography buffers refill pack
(#166-0016EDU) includes binding
buffer, column equilibration buffer,
column wash buffer, elution buffer
57.00
HIC chromatography columns and
caps, 8 each (#166-0413EDU)
41.00
LB nutrient agar powder,
20 g (#166-0600EDU) or
12.00
500 g (#166-0472EDU)
89.00
Petri dishes, 60 mm, sterile, 500
128.00
(#166-0470EDU)
Inoculation loops, 10 µl, sterile, 80
13.00
(#166-0471EDU)
Jellyfish foam floating racks, 8 racks
30.00
(#166-0479EDU)
Disposable plastic transfer pipets,
105.00
sterile, 500 (#166-0474EDU)
Cell culture tubes, 17 x 100 mm, 14 ml, 17.00
sterile, 25 (#166-0476EDU)
Clear polystyrene tubes, 13 x 100 mm, 39.20
9 ml, 1,000 (#223-9750EDU)
67.20
Poly-Prep® columns, empty, 50
(#731-1550EDU)
Macro-Prep® HIC Support, 100 ml
295.20
(#156-0080EDU)
Lab 4
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25
Microbes and Health Kit: “What Causes Yogurtness?”™
Microbes and Health Kit
Each kit supports 32 students.
Microbes and Health Kit
Catalog #
List Price . . . . . . . . .EDU Price
166-5030EDU
$123.75 . . . . . . . . .$99.00
Students will use microscopes, agar plates, and their powers of observation
to identify the bacteria used to produce yogurt and to provide proof for their hypothesized
identification. Use this kit to examine metabolism, cellular chemistry, and the role of bacteria
in both disease and food microbiology.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Ships and stores at room temperature.
Key Kit Features
• Isolate yogurt-causing bacteria
• Culture bacteria from yogurt
• Follow Koch’s postulates
• Learn laboratory microbiology skills
• Study food microbiology and bacterial
metabolism
• Complete in three 45 minute lab sessions
Bacterial colonies cultured from yogurt (below).
Streptococcus thermophilus (inset).
• Interpretation of
experimental results
• Use of experimental
controls
• Evaluation of hypothesis
• Microscopy
Ecosystems,
Environment,
and
Interactions
• Bacterial metabolism
• Prokaryotic cell structure
and cell division
• Effects of temperature and
pH on bacterial growth
• Antibiotics
Science and
Engineering
Practices
Microbes
and
Health
Kit
Cellular
Processes
Evolution
Lab Preparation Checklist
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
Discover the cause of disease. In the 18th century bacterial diseases were still a
deadly mystery. Bacteria were sometimes found in diseased humans and animals — but
did the bacteria cause the disease or did the bacteria merely follow a disease caused by
another unknown agent? To know the cause is the first step toward cure or prevention.
Join Robert Koch, Louis Pasteur, and the founders of modern microbiology in a thrilling
search to find the bacterial culprit behind a new disease. The new disease examined in
this lab is “yogurtness” — an affliction of “healthy” milk that causes it to become acidic
and thick. What is the cause of yogurtness? Can you use Koch’s postulates, the standard
of proof in the identification of microbial disease agents, to identify the guilty microbe in
this inquiry-based activity?
• Epidemiology and
disease
• Microbiology
CLASSROOM KITS: MICROBES AND HEALTH KIT
The chemistry of the bacterial cell is brought into focus as students examine
bacteria and their interaction with the environment. Enzyme-catalyzed chemical reactions
in bacteria provide energy for the bacteria as they change food into secreted waste
products. In some cases, bacterial waste products can be the cause of disease symptoms,
and in other cases they may create foods and nutrients for people. Thus bacteria can
sometimes be our friends and other times be our foes. For a long time, biotechnology has
utilized friendly bacteria in the production of foods such as cheese, sauerkraut, kimchi,
coffee, sour cream, vinegar, sausage, and yogurt. Other bacteria cause cholera, typhus,
leprosy, tuberculosis, and anthrax. In this lab students will examine both the risks and
benefits of bacteria to better understand their role in disease and food production.
Ampicillin, lyophilized
LB nutrient agar powder
LB broth capsules
E. coli strain HB101 K-12, lyophilized
Petri dishes, 60 mm, sterile
Cell culture tubes, 15 ml, sterile
Inoculation loops, sterile
Disposable plastic transfer pipets
Curriculum, including teacher’s guide,
student manual, and graphic quick guide
2
1
12
1
40
75
80
10
1
Required Accessories Not Included in Kit:
Incubation oven, p. 137
1
Microscopes
2–8
pH paper strips (range 4–7)
48
Yogurt
2–4 types
Milk
400 ml
Toothpicks
Lab 1
Recommended (Optional) Accessories:
Magnifying glasses
Microwave oven
Refresh Kit Components: (more info pp. 84, 143)
Microbial culture kit reagent refill pack $48.00
(#166-5021EDU) includes
ampicillin, LB broth capsules (12),
LB nutrient agar powder,
E. coli strain HB101 K-12
LB nutrient agar powder,
20 g (#166-0600EDU) or
12.00
500 g (#166-0472EDU)
89.00
Ampicillin (#166-0407EDU)
17.00
E. coli strain HB101 K-12
18.00
(#166-0408EDU)
Petri dishes, 60 mm, sterile, 500
128.00
(#166-0470EDU)
Inoculation loops, 10 µl, sterile, 80
13.00
(#166-0471EDU)
Disposable plastic transfer pipets,
105.00
sterile, 500 (#166-0474EDU)
Cell culture tubes, 17 x 100 mm, 14 ml, 17.00
sterile, 25 (#166-0476EDU)
15 ml tube racks, holds 60 tubes,
72.00
set of 5 racks (#166-0483EDU)
Lab 2
Lab 3
Molecules
to Organisms
Genetics and
Heredity
• Effects of pH on
proteins
• Enzymes
• Protein structure
and function
• Variation in bacteria
• Adaptation to environment
• Bacterial defense mechanisms
26
1-800-4BIORAD (1-800-424-6723)
Visit us on the Web at explorer.bio-rad.com
27
Classroom Kits: Protein Analysis Kits
Section Contents
Biofuel Enzyme Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Size Exclusion Chromatography Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Got Protein?™ Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
ELISA Immuno Explorer™ Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Comparative Proteomics Kit I: Protein Profiler Module . . . . . . . . . . . . . . . . 38
Comparative Proteomics Kit II: Western Blot Module . . . . . . . . . . . . . . . . . 40
New Rapid Blotting — V3 Western Workflow™:
Stain-Free Rapid Blotting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CLASSROOM KITS: PROTEIN ANALYSIS KITS
Protein Analysis Kits
“
Bio-Rad kits have been
classroom changers. My
students step into college
labs all over the country
with a knowledge level that
is above most students.
This has been possible
by using Bio-Rad kits
and products.
”
Pat Boehmer
Carrington High School
Carrington, North Dakota
2>
1>
28
3>
1-800-4BIORAD (1-800-424-6723)
4>
5>
1. Santa Fe High School, Alachua, Florida; 2. Tracy High School, Tracy, California;
3. Bio-Rad Teacher Training, Mexico City, Mexico; 4. University of North Carolina,
Chapel Hill, North Carolina; 5. Silver Creek High School, San Jose, California.
29
Biofuel Enzyme Kit — AP Big Idea 4: Can Enzymes Solve Global Warming?
Biofuel Enzyme Kit
Each kit supports 32 students for all 6 activities.
Cellulosic ethanol is a biofuel derived from plant matter. Cellulose is a polysaccharide found
in the cell walls of plants. The breakdown of cellulose into sugar is a multistep process that
is facilitated by a family of enzymes called cellulases. Each cellulase has its unique role in
processing cellulose from a long strand of glucoses down to single units that can then
undergo microbial fermentation to produce ethanol.
Biofuel Enzyme Kit
Catalog #
List Price . . . . . . . . .EDU Price
166-5035EDU
$158.75 . . . . . . . .$127.00
Ships at room temperature.
Store at 4°C.
Key Kit Features
• Aligns with AP Biology Big Idea 4; Lab 13
• Guides instruction on enzyme kinetics and
biofuel energy sources
• Contains no strong oxidizing agents
• Incurs no expensive hazardous waste
disposal costs
• Enables both qualitative and quantitative
measurement of reactions
• Complete core activity in one 45 minute
session
• Includes 5 additional enrichment activities
Enzymes and Energy
Reveal the power of enzyme kinetics by illustrating the theory through the real-world
application of biofuel production. The biofuel enzyme kit tests the ability of an enzyme
to increase the rate of conversion of a clear substrate to a colored product. Students
will test and calculate the rate of conversion of a sugar substrate ( p-nitrophenyl
glucopyranoside) to p-nitrophenol and glucose in the presence or absence of the enzyme
cellobiase (part of the cellulase family). After establishing the rate of reaction in the presence
of the enzyme, various conditions influencing the reaction rate can be tested. In addition,
students can perform independent inquiry with mushroom extracts and consider ecology and
evolution of mushrooms.
Activity 1: Determine the reaction rate in the presence or absence of an enzyme
Activity 2: Determine the effect of temperature on the reaction rate
Activity 3: Determine the effect of pH on the reaction rate
Activity 4: Determine the effect of enzyme concentration on the reaction rate
Activity 5: Determine the effect of substrate concentration on the reaction rate
Activity 6: Test the ability of mushroom extracts to increase the reaction rate
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Can enzymes solve global warming?
Let your students decide whether this is possible!
•
•
•
•
Ecosystems,
Environment,
and
Interactions
•
•
•
•
Cellobiase activity
Enzymatic production of energy
Chemical bonds and energy
Energy requirements of living
systems
• Function of
cellobiase
• Thermophilic
life forms
30
1-800-4BIORAD (1-800-424-6723)
Lab Preparation Checklist
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
Fueling Our Future
Fossil fuels are based on a natural decomposition process, so the fuel you use today
might be several million years old! The need for energy is outpacing the supply as
markets globalize, more countries become industrial contributors, and populations
increase. As the overall demand for energy rises, science is at the forefront of identifying
potential fuel sources. Solar, hydrogen, wind, nuclear, and biofuels are just a few possible
renewable fuel supplies that could replace nonrenewable petroleum.
• Biofuels
• Carbon cycle
• Energy flow through
the ecosystem
• Energy facilitates the
cycling of molecules
• Conservation of matter
Cellular
Proccesses
Quantitation of enzyme activity
Use of a spectrophotometer
Use of experimental controls
Creation and use of a
standard curve
Science and
Engineering
Practices
Biofuel
Enzyme
Kit
Evolution
CLASSROOM KITS: BIOFUEL ENZYME KIT — AP BIG IDEA 4
Enzymes are a core biological function that can be applied across several disciplines
and taught at a variety of levels. This kit allows you to tailor your content with single or
multiple activities.
Molecules to
Organisms
Genetics and
Heredity
• Chemical and physical properties
of enzymes
• Protein chemistry and structure
• Chemistry of the substrate
• Properties of chemical bonds
• Effects of pH, temperature, and
concentration on enzyme activity
• Michaelis-Menten
Enzyme, cellobiase, 400 µl
1
Substrate, p-nitrophenyl glucopyranoside,
1
90 mg
Standard, p-nitrophenol (1 mM, 4 ml)
1
2x stop solution, 100 ml
1
10x resuspension buffer, 50 ml
1
Extraction buffer, 50 ml
1
Disposable plastic transfer pipets
40
1.5 ml microcentrifuge tubes
90
15 ml conical tubes
50
1.5 ml standard disposable polystyrene
1
cuvettes, 100
Curriculum, including teacher’s guide,
1
student manual, and graphic quick guide
Required Accessories Not Included in Kit:
Deionized or distilled water
1L
Timers or stopwatches
8
Activity 2: Effects of Temperature
Thermometers
8
Activity 6: Mushroom Extracts
Mortars and pestles
Balance
8
1
Recommended (Optional) Accessories:
Adjustable micropipets, p. 138
1–8
100–1,000 µl
Pipet tips, p. 140
1 bag
100–1,000 µl, BR-40
Spectrophotometer, p. 131
Water bath or dry bath, pp. 136–137
DyNA Chill cooler, p. 136
Incubation oven, p. 137
Centrifuge, p. 134
Refresh Kit Components: (more info pp. 84, 143)
Biofuel enzyme kit temperature$79.00
sensitive reagent refill pack
(#166-5036EDU) includes enzyme
(cellobiase), substrate (p-nitrophenyl
glucopyranoside), standard
(p-nitrophenol), 2x stop solution, 10x
resuspension buffer, extraction buffer
1.5 ml standard disposable polystyrene
cuvettes, 100 (#223-9955EDU)
Conical centrifuge tubes, 15 ml, 50
(#166-0475EDU)
Disposable plastic transfer pipets,
nonsterile, 500 (#166-0480EDU)
1.5 ml EZ Micro™ test tubes, 500
(#223-9480EDU)
Green racks, set of 5 racks
(#166-0481EDU)
15 ml tube racks, holds 60 tubes,
set of 5 racks (#166-0483EDU)
Cuvette racks, holds 12 standard
size cuvettes, set of 5 racks
(#166-0485EDU)
9.60
26.00
52.00
23.20
50.01
72.00
56.00
Lab 1
• DNA > RNA > Protein > Activity (Trait)
• Biochemistry of mushrooms,
ruminants, and bacteria
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31
Size Exclusion Chromatography Kit: Sizing Up the Situation
Size Exclusion Chromatography Kit
Column chromatography is the most common method used in biotechnology research
and industry for separating a mixture of components in a liquid. Biochemists and molecular
biologists use a variety of chromatography techniques to isolate and purify proteins, DNA,
or other molecules from a mixture.
Lab Preparation Checklist
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
This method of separating one substance from a complex mixture is based on
the physical and chemical characteristics of the molecules of interest — properties such as
molecular weight, electrical charge, or solubility in various solvents. Successful separation
of a molecule of interest by chromatography requires that it be sufficiently different from
the majority of other components in the mixture in one or more of these properties to be
distinguished from them. Size exclusion chromatography separates solubilized molecules
such as proteins by their size, or molecular weight, from both larger and smaller
contaminating proteins.
Each kit supports 32 students.
Size Exclusion Chromatography Kit
Catalog #
List Price . . . . . . . . .EDU Price
166-0008EDU
$102.50 . . . . . . . . .$82.00
Convenient lyophilized reagents.
Ships and stores at room temperature.
In this lab, a mixture of molecules in solution is applied to a chromatography column containing
microscopic porous beads. As the solution flows through the column, the larger molecules
bypass and flow around the beads. They are “excluded” from entering the pores by their size,
and flow through the column with relative speed. The smaller molecules enter the porous
beads, and are impeded in their flow through the column. The different rates of travel of the
solutes through the column lead to effective separation.
Key Kit Features
• Separate a mixture of biomolecules
• Determine the number of components
in a mixture
• Study the chemical and physical properties
of biomolecules
• Apply the principles of chromatography
• Complete in one 45 minute lab session
This kit utilizes the colored molecules hemoglobin (reddish-brown) and vitamin B12 (pink).
The contrasting colors are easily visible as the molecules pass through the column and into
collection tubes at different rates due to their molecular weights.
1
Hemoglobin/vitamin B12 sample mixture
Bio-Gel® P-60 columns with caps
8
Disposable plastic transfer pipets
10
Column chromatography buffer, 50 ml
1
Sample collection tubes, 5 ml
100
Curriculum, including teacher’s guide,
1
student manual, and graphic quick guide
Refresh Kit Components: (more info pp. 84, 143)
Sample mixture and column
$42.00
chromatography buffer
(#166-0018EDU)
Electron micrograph showing individual
size exclusion chromatography beads.
Bio-Gel P-60 columns with caps, 8
36.00
(#166-0020EDU)
Disposable plastic transfer pipets,
52.00
nonsterile, 500 (#166-0480EDU)
Clear polystyrene tubes, 13 x 100 mm, 39.20
9 ml, 1,000 (#233-9750EDU)
15 ml tube racks, holds 60 tubes,
72.00
set of 5 racks (#166-0483EDU)
Bio-Gel P-60 gel, medium, 100 g
(#150-4160EDU)
Poly-Prep® columns, empty, 50
(#731-1550EDU)
259.20
67.20
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Vitamin B12 — mass 1,350 daltons
Hemoglobin — mass 65,000 daltons
CLASSROOM KITS: SIZE EXCLUSION CHROMATOGRAPHY KIT
This chromatography kit teaches the basic principles of size exclusion chromatography.
• Separation and isolation
of biomolecules
• Interpretation of
experimental results
Ecosystems,
Environment,
and
Interactions
• Sickle cell anemia
• Malaria and effect on
red blood cells
• Vitamin requirements
and related diseases
• Physiology of vitamins
Science and
Engineering
Practices
Size
Exclusion
Chromatography
Molecules to
Organisms
Kit
• Red blood cell structure
• Respiration and
metabolism
• Selective advantage of
heterozygous alleles
• Genetic mutation
• Mechanisms of evolution
• Natural selection
32
1-800-4BIORAD (1-800-424-6723)
Cellular
Proccesses
Evolution
Genetics and
Heredity
•
•
•
•
• Chemical and physical properties
of biological molecules
• Protein chemistry and structure
• Hemoglobin structure and function
• Vitamin B12 structure and function
• Chromatographic separation of
molecules
Lab 1
DNA > RNA > Protein > Trait
Inherited disorders
Molecular basis of sickle cell disease
DNA mutations
Visit us on the Web at explorer.bio-rad.com
33
Got Protein?™ Kit: What You See Is What You Get!
Got Protein? Kit
Lab Preparation Checklist
Kit contains sufficient materials for 80 student
workstations (2–4 students per workstation).
Based on the Bio-Rad Quick Start™ Bradford protein assay, this inquiry-based
biophotonics lab allows students to analyze and compare the protein content in milk,
sports drinks, egg, muscle tissue, saliva, tears, or any source of soluble, biologically derived
material. Protein quantitation is often necessary before isolation, separation, and analysis by
chromatography, electrophoresis, or western blotting. This lab integrates biology, chemistry,
and physics, allowing students to develop an understanding about how the chemical,
physical, and biological properties of proteins determine their structure and function.
Each kit supports 320 students.
It is impossible to place biological material under a microscope and count the number of
protein molecules per unit volume the way we can count the number of cells. Therefore,
something measurable that is proportional to the concentration of the substance of interest
must be identified. Beer’s law states that when a solute absorbs light of a particular
wavelength, the absorbance is directly proportional to the concentration of substance in
solution. The measurement most commonly used in protein assays is the absorbance of light.
Got Protein? Kit
Catalog #
List Price . . . . . . . . .EDU Price
166-2900EDU
$218.75 . . . . . . . .$175.00
Standards ship on blue ice.
Store at 4°C.
However, proteins do not absorb sufficient light to assay — by themselves.
Key Kit Features
• Explore biophotonics
• Study protein structure/function
• Apply Beer’s law
• Measure protein concentrations
• Learn spectrophotometry
• Complete in one 45 minute lab session
The Bradford method is based on the color development formed when the dye
Coomassie Blue G-250 binds to protein. The unique chemical properties of the dye allow it
to interact with the side chains, or R-groups, of specific amino acids. There is a correlation
between the amount of blue color and the amount of protein in the sample: the more
protein, the more intense the blue color. The simplicity of the assay allows the results to
be measured qualitatively by eye or quantitatively with a spectrophotometer.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
In this lab, students use absorbance values from a set of protein samples with known
concentrations to create a standard curve on linear graph paper. Protein concentrations
of their test samples can then be extrapolated by hand or plotted using a graphing utility
such as Microsoft Excel. Students also learn to use a spectrophotometer, micropipet, and
computer, which are all invaluable tools in modern bioscience research.
Ecosystems,
Environment,
and
Interactions
• Lactose intolerance
• Mineral and vitamin
requirements
• Function of milk proteins
• Role of milk in reproductive
success of organisms
• Natural selection
34
1-800-4BIORAD (1-800-424-6723)
Quick Start Bradford protein assay kit 4,
includes 1x dye reagent (1 L), bovine
γ-globulin standard set (2 sets of
7 standards, 0.125–2.0 mg/ml, 2 ml)
10x PBS, 100 ml
1.5 ml standard disposable polystyrene
cuvettes, 100
Curriculum, including teacher’s guide,
student manual, and graphic quick guide
1
1
1
1
Required Accessories Not Included in Kit:
Adjustable micropipets, p. 138
2–20 µl
1–8
100–1,000 µl
1
Pipet tips, p. 140
2–20 µl, BR-35
1 box
100–1,000 µl, BR-40
1 box
Microcentrifuge tubes, 1.5 ml , p. 143
1 bag
Conical centrifuge tubes, 15 ml, p. 143
8
Distilled water
100 ml
Milk samples (suggestions: low fat,
10 ml
fat free, soy, baby formula, etc.)
Recommended (Optional) Accessories:
SmartSpec™ Plus spectrophotometer,
p. 131
1
Refresh Kit Components: (more info pp. 84, 143)
Quick Start Bradford protein assay
$144.80
kit 4 (#500-0204EDU) includes
1x dye reagent (1 L), bovine
γ-globulin standard set (2 sets of
7 standards, 0.125–2.0 mg/ml, 2 ml)
10x PBS, 100 ml (#166-2403EDU)
15.00
1.5 ml standard disposable polystyrene
9.60
cuvettes, 100 (#223-9955EDU)
Conical centrifuge tubes, 15 ml, 50
26.00
(#166-0475EDU)
Green racks, set of 5 racks
50.01
(#166-0481EDU)
Cuvette racks, holds 12 standard size 56.00
cuvettes, set of 5 racks (#166-0485EDU)
•
•
•
•
• Protein production
and secretion
• Nutrition and immunity
CLASSROOM KITS: GOT PROTEIN? KIT
The Got Protein? kit is designed to introduce students to proteomics and provides the
tools for them to develop their own protein-based experiments.
Science and
Engineering
Practices
Got
Protein?
Kit
Cellular
Proccesses
Evolution
Quantification of milk proteins
Use of a spectrophotometer
Use of experimental controls
Creation and use of a standard curve
Lab 1
Molecules to
Organisms
Genetics and
Heredity
• Chemical and physical properties
of proteins
• Biophotonics and Beer’s law
• Protein chemistry and structure
• Chemistry of dye molecules
• Properties of chemical bonds
• DNA > RNA > Protein > Trait
• Biochemistry of milk
Visit us on the Web at explorer.bio-rad.com
35
ELISA Immuno Explorer™ Kit: Real Antibodies, Real Learning, Real Fun
ELISA Immuno Explorer Kit
Each kit supports 48 students.
ELISA Immuno Explorer Kit
Catalog #
List Price . . . . . . . . .EDU Price
166-2400EDU $168.75 . . . . . . .$135.00
Convenient lyophilized reagents.
Ships at room temperature.
Store at 4°C.
How this kit works: Three approaches to ELISA may be taken with this kit.
Curriculum guides and instructions for each protocol are included in the kit, complete
with teacher’s guides and student manuals. Select the type of test most relevant to your
current course work.
1. ELISA for disease outbreaks — tracking the spread of disease through a
classroom exchange of simulated body fluids.
Test applications: HIV, bird flu, West Nile, and swine flu viruses, common cold, cholera,
smallpox, anthrax, influenza, and STD detection.
2. ELISA for detecting antigens in a sample.
Test applications: Pregnancy hormone, drug, allergen, and GMO discovery; air, food,
and water testing; HIV, mad cow disease, and smallpox, West Nile, bird flu, and swine
flu virus detection.
36
1-800-4BIORAD (1-800-424-6723)
60
75
1
Recommended (Optional) Accessories:
Microplate reader with printer, p. 133
HRP enzyme substrate (TMB), 25 ml
(#166-2402EDU)
10x PBS, 100 ml (#166-2403EDU)
Microplate with 12-well strips
(8 rows of 12 wells), 3 plates
(#166-2405EDU)
Disposable plastic transfer pipets,
nonsterile, 500, (#166-0480EDU)
Antigen (chicken γ-globulin)*
(#166-2406EDU)
Primary antibody (rabbit anti-chicken
polyclonal antibody)* (#166-2407EDU)
Secondary antibody (goat anti-rabbit
antibody conjugated to horseradish
peroxidase, or HRP)* (#166-2408EDU)
Green racks, set of 5 racks
(#166-0481EDU)
27.00
15.00
30.00
52.00
32.00
32.00
32.00
50.01
10% Tween 20, p. 125
Ecosystems,
Environment,
and
Interactions
Science and
Engineering
Practices
ELISA
Immuno
Explorer
Kit
Evolution
1
1
1
80
3
Refresh Kit Components: (more info pp. 84, 143)
ELISA kit reagent refill pack
$101.00
(#166-2401EDU) includes antigen,
primary antibody, secondary
antibody, 10% Tween 20, 10x PBS,
HRP enzyme substrate
An antigen is a substance that stimulates an immune response and the production of antibodies. Antigens are
usually proteins, but can be any type of molecule.
• Animal immune systems
response
• HIV mutation and evolution
• Viral drug resistance
• Biowarfare in nature
1
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
• Tapping nature’s toolkit to
solve human problems
• Use of immunodetection to
hunt for proteins
• Use of positive and negative
experimental controls
• Interpretation of experimental
results
Cellular
Proccesses
1
1
Required Accessories Not Included in Kit:
Adjustable micropipets, 20–200 µl, p. 138 12
Or fixed volume micropipets, 50 µl, p. 138 12
Pipet tips, 2–200 µl, BR-35, p. 140
1 bag
This kit integrates multiple core content areas and facilitates teaching both about immune
system functions and about the unique properties of antibodies that have revolutionized
medicine, epidemiology, and life science research.
• Immune response
• Manufacturing antibodies
• Virology and immunology
Antigen (chicken γ-globulin)*
Primary antibody (rabbit anti-chicken
polyclonal antibody)*
Secondary antibody (goat anti-rabbit
antibody conjugated to horseradish
peroxidase, or HRP)*
HRP enzyme substrate (TMB), 25 ml
10x PBS, 100 ml
10% Tween 20, 5 ml
Disposable plastic transfer pipets
Microplates with 12-well strips
(8 rows of 12 wells)
Yellow microcentrifuge tubes, 2.0 ml
Colored microcentrifuge tubes, 2.0 ml
Curriculum, including teacher’s guide,
student manual, and graphic quick guide
Key Kit Features
• Apply a genuine diagnostic procedure
• Simulate real-world HIV testing
• Simulate GMO, pregnancy, and drug testing
• Complete in one 45 minute lab session
3. ELISA for detecting antibodies in a blood sample for past exposure to a disease.
Test applications: Lyme disease, trichinosis, HIV, West Nile virus, bird flu virus, and
swine flu virus detection.
• HIV, mad cow disease,
and virus testing
• Epidemiology and biodefense
• Drug, pregnancy, and GMO
testing
• Soil, water, air testing
Lab Preparation Checklist
Kit contains sufficient materials for 12 student
workstations (2–4 students per workstation).
What is ELISA? The enzyme-linked immunosorbent assay (ELISA for short) is a technique
used to detect the presence of an antibody or an antigen* in a sample. It harnesses
antibodies’ ability to act like magic bullets and attach themselves to their targets (antigens).
An ELISA utilizes two antibodies, one of which is specific to the antigen and another that
is coupled to an enzyme. This second antibody gives the assay the enzyme-linked part of
its name, and will cause a chromogenic substrate to produce color wherever it is bound.
ELISA is a powerful antibody-based biodetection tool used in the field to hunt for pathogens
in water, food, or air, whether they emerge naturally or through acts of aggression.
*
CLASSROOM KITS: ELISA IMMUNO EXPLORER KIT
Use real antibodies to see how a real ELISA works. Explore biodefense,
immunology, or health science with this topical, hands-on lab. Simulate the outbreak
of a disease in your classroom and use real antibodies to track it. Teach your students
how protein structure and enzyme substrate interactions are used to detect HIV, bird flu,
genetically modified organisms (GMOs), and the molecular markers of cancer, pregnancy,
or drug use.
* Convenient lyophilized reagents.
Molecules to
Organisms
Genetics and
Heredity
• Enzyme-substrate
interactions
• Protein structure
and function
• Properties of antigens
and antibodies
• DNA > RNA > Protein (Antibodies)
• Antibody structure and function
• Antibody production via genetic
recombination
Extra Curriculum for ELISA on the Web
Download the Biological Bodyguards
curriculum that embeds the
ELISA Immuno Explorer lab in topical
case study scenario format, developed by
the University of North Carolina, Chapel Hill
DESTINY Traveling Science Learning Program.
Download the complete PDF free at
explorer.bio-rad.com.
Lab 1
Visit us on the Web at explorer.bio-rad.com
37
Comparative Proteomics Kit I: Protein Profiler Module — AP Big Idea 1: Does Molecular Evidence
Kit I: Protein Profiler Module
The protein profiler module moves beyond DNA and allows students to employ
protein electrophoresis, the most widely used technique in life science research, to study
protein structure and function. Students learn to use SDS-PAGE to generate protein profiles
from the muscles of both distantly and closely related species of fish. From their results,
they compare the different species’ profiles to test the hypothesis that protein profiles can
be indicators of evolutionary relatedness.
Each kit supports 32 students.
Kit I: Protein Profiler Module
This kit allows your students to explore evolution at the molecular level within the context
of the central molecular framework of biology:
Catalog #
List Price . . . . . . . . .EDU Price
166-2700EDU
$218.75 . . . . . . . .$175.00
Kits I and II: Protein Profiler and
Western Blot Modules
166-2850EDU
453.75 . . . . . . . . .363.00
DNA ➤ RNA ➤ Protein ➤ Trait — Phenotype
Convenient lyophilized reagents. Ships at room
temperature. Immediately store temperature-sensitive
reagents at –20°C as indicated.
Obtain fish samples locally. Mini-PROTEAN TGX precast
gels (12 month shelf life) available separately.
Changes in proteins can reflect changes in the gene pool. Actin and myosin are the major
muscle proteins essential for locomotion and survival in all animals. Muscle consists mainly
of actin and myosin, but numerous other proteins also compose muscle tissue. The
structures and functions of actin and myosin have remained relatively stable or “conserved”
in all animals over evolutionary time. However, other muscle proteins exhibit considerable
variation, even among closely related species. Detectable variations between organisms’
protein profiles reflect physiological adaptations to different environments, but they originate
as random DNA mutations. Such mutation events, if favorable, persist through the natural
selection process and contribute to the evolution of species with new specialized functions.
Key Kit Features
• Aligns with AP Biology Big Idea 1; Lab 3
• Explore evolution
• Study protein structure/function
• Apply protein electrophoresis
• Construct cladograms
• Complete in three 45 minute lab sessions
Mutation ➤ Variation ➤ Specialization ➤ Speciation — Evolution
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
This is an open-ended, inquiry-based kit. Students make predictions about their results in
prelab activities using Internet databases and published phylogenetic information. They
generate novel results and apply their findings directly to the problem of solving evolutionary
relationships by constructing cladograms (phylogenetic trees). From their gel data, they build
up a tree and assign each organism a branch. Students can decide whether their results
support their predictions.
• Use of gel electrophoresis to
fingerprint proteins
• Use of experimental controls
• Creation of standard curves
and cladograms
• Interpretation of experimental
results
The kit guides students through the thought processes involved in a laboratory-based
scientific investigation. Students are asked: Can molecular evidence support the theory
of evolution? Why or why not? What explanations can you suggest?
Ecosystems,
Environment,
and
Interactions
• Interdependence of
organisms
• Detecting and analyzing
variations in proteins
• Comparative proteomics
• Eukaryotic cell structure
and function
• Muscle structure and
function
• Cladistics and phylogenetic
relationships
• Biodiversity and natural
selection
• Evolution of adaptive traits
• Physical environment as
selective force
38
1-800-4BIORAD (1-800-424-6723)
Science and
Engineering
Practices
Protein
Profiler
Module
Cellular
Proccesses
Evolution
Lab Preparation Checklist
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
Proteomics asks the question: What do our genes do? Genes encode proteins
that determine an organism’s form, function, and phenotype — the raw material of natural
selection. Proteomics is the study of the structure, function, and interaction of proteins with
each other and with their environment.
Mini-PROTEAN® TGX™ gel, 4–20%
polyacrylamide precast gel runs
in the Mini-PROTEAN Tetra
cell (see page 120)
CLASSROOM KITS: COMPARATIVE PROTEOMICS KIT I: PROTEIN PROFILER MODULE — AP BIG IDEA 1
The Human Genome Project has completed the task of sequencing all human genes.
Far from closing the book, this body of work opened up a whole new field: proteomics.
Support the Theory of Evolution?
Molecules to
Organisms
Genetics and
Heredity
• Chemical and physical
properties of proteins
• Protein structure (1°, 2°, 3°, 4°)
and function
• Protein extraction techniques
• Chemistry of protein
electrophoresis
• DNA > RNA > Protein > Trait
• Molecular vs. morphological classification
• Post-transcriptional and post-translational
modification
• Phylogenetic and cladistic analyses
Laemmli sample buffer, 30 ml
1
Precision Plus Protein™ Kaleidoscope™
1
standards, 50 µl
10x Tris/glycine/SDS electrophoresis
1
buffer, 1 L
Bio-Safe™ Coomassie stain for proteins, 100 ml 2
Actin and myosin standard, 500 µg lyophilized 1
Dithiothreitol (DTT), 0.3 g
1
Prot/Elec™ pipet tips for gel loading
1 rack
1.5 ml fliptop microcentrifuge tubes
60
1.5 ml screwcap microcentrifuge tubes
50
Disposable plastic transfer pipets
30
Curriculum, including teacher’s guide,
1
student manual, and graphic quick guide
Required Accessories Not Included in Kit:
Fish samples
5–8 types
Adjustable micropipets, 2–20 µl, p. 138
8
Power supplies, p. 130
2–4
Water bath or dry bath, pp. 136–137
1
Gel staining trays, p.143
4–8
Foam floating racks, p.143
8
Lab 1
If Using Polyacrylamide Gel Electrophoresis:
Required Accessories
Vertical gel electrophoresis chambers, p.120 2–4
Mini-PROTEAN TGX precast polyacrylamide 8
gels, 4–20%, 10-well each, p. 123
If Using Agarose Gel Electrophoresis:
Required Accessories
Horizontal gel electrophoresis chambers, 4–8
p. 104
Low-melt agarose, 25 g, p. 106
Pipet tips, 2–20 µl, TBR-35, p. 140
Acetic acid, 100 ml
Reagent alcohol/ethanol, 400 ml
Lab 2
Recommended (Optional) Accessories:
Sample loading guides, p. 121
GelAir™ drying system, p. 121
Refresh Kit Components: (more info pp. 84, 143)
Protein profiler temperature-sensitive
$74.00
reagent refill pack (#166-2701EDU),
includes Precision Plus Protein
Kaleidoscope standards, actin and
myosin standard, and DTT
Actin and myosin standard
(#166-0010EDU)
Gel staining trays, 4 (#166-0477EDU)
Jellyfish foam floating racks, 8 racks
(#166-0479EDU)
25.00
16.00
30.00
Laemmli sample buffer, p. 125
Precision Plus Protein Kaleidoscope
standards, 500 µl, p. 126
10x Tris/glycine/SDS electrophoresis buffer, p. 125
Bio-Safe Coomassie stain, p. 127
DTT, p. 125
Lab 3
Extra Curriculum for Proteomics on the Web
Download From Finches to Fishes curriculum
that embeds the Protein Profiler lab in topical
case study scenario format, developed by the
University of North Carolina, Chapel Hill DESTINY
Traveling Science Learning Program. Download
the complete PDF free at explorer.bio-rad.com.
Visit us on the Web at explorer.bio-rad.com
39
Comparative Proteomics Kit II: Western Blot Module: Tapping Nature’s Toolkit
Kit II: Western Blot Module
Western blotting is used extensively in research to determine the presence of specific proteins,
to quantitate their expression levels, and to determine whether they have undergone genetic or
post-translational modifications. This surefire method categorically identifies proteins of interest
based on two distinguishing features: molecular mass and antibody binding specificity.
Each kit supports 32 students.
Kit II: Western Blot Module
Myosin is a major muscle protein essential for locomotion and survival in all animals. The
essential structure and function of myosin has remained relatively stable or “conserved”
in all animals over evolutionary time. However, differences in the molecular weights of the
myosin light chain proteins of different species are detectable via western blotting, enabling
students to hypothesize about how these variations relate to their evolutionary relationships.
Catalog #
List Price . . . . . . . . .EDU Price
166-2800EDU
$248.75 . . . . . . . .$199.00
Kits I and II: Protein Profiler and Western
Blot Modules
166-2850EDU
453.75 . . . . . . . . .363.00
Convenient lyophilized reagents. Ships at room
temperature. Immediately store temperature-sensitive
reagents at –20°C as indicated. Obtain fish samples
locally. Mini-PROTEAN® TGX™ precast gels (12 month
shelf life) available separately. Protein profiler module
required.
This western blot module allows your students to take protein profiling to the next level.
Students use western blotting to specifically identify myosin light chain from the hundreds
of other proteins that make up the muscle cell extracts of closely and distantly related
species of fish.
Key Kit Features
• Explore immunodetection
• Explain HIV detection
• Apply immunology
• Antibodies as tools
• Complete in four 45 minute lab sessions
In the first part of this laboratory (protein profiler module), students generate protein profiles
and visualize the unique arrays of proteins composing the muscle tissues from each of their
samples. From their protein gel results, students make educated guesses as to the identities
of the proteins. However, based on their relative molecular masses alone these inferences
remain guesses. Via western blotting, the protein bands in their polyacrylamide gels are
transferred horizontally to a membrane and an anti-myosin light chain antibody is employed
to precisely identify which protein in each species’ profile is myosin light chain.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Using Internet-based bioinformatics databases, students can compare their experimentally
determined results to actual protein sequence data derived from DNA and RNA sequences
and consider whether variations in myosins between species are due to “genetic” or
“epigenetic” factors.
• Use of immunodetection to
identify specific proteins
• Use of experimental controls
• Creation and use of
standard curves
• Use of bioinformatics
databases
Western blot of protein profiler gel
reveals precise location of
myosin light chain proteins.
Ecosystems,
Environment,
and
Interactions
• Posttranscriptional and
posttranslational modification
• Muscle protein structure and
function
• Adaptations to environment
• Immunology and virology
• Molecular variation
• Evolution of adaptive traits
• Environment and natural
selection
• Phylogenetic relationships
40
1-800-4BIORAD (1-800-424-6723)
Science and
Engineering
Practices
Western
Blot
Module
Cellular
Proccesses
Evolution
Lab Preparation Checklist
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
Western blotting employs antibodies to pinpoint specific proteins of interest in complex
protein mixtures such as cell extracts. Because of its accuracy, western blotting is used as the
confirmatory diagnostic test for HIV and mad cow disease (bovine spongiform encephalopathy).
• Ecosystems, symbiosis,
and interdependence
• Real-world HIV, mad cow
disease, and virus testing
CLASSROOM KITS: COMPARATIVE PROTEOMICS KIT II: WESTERN BLOT MODULE
When foreign invaders are detected, animal immune systems naturally generate
antibodies and tag them for destruction. The ability of antibodies to act like magic bullets and
target viral, bacterial, and allergenic antigens in the body also makes them ideal tools in the
hunt for specific molecules in bioscience research and diagnostic tests.
Molecules to
Organisms
Genetics and
Heredity
• Chemical and physical
properties of proteins
• Antibody structure and function
• Protein extraction techniques
• Enzyme-substrate interactions
• Chemistry of electrophoresis
and blotting
• DNA > RNA > Protein > Trait
• Antibody production via genetic
recombination and gene splicing
• Genetic and epigenetic factors
Primary antibody (anti-myosin light
1
chain mouse monoclonal), lyophilized
Secondary antibody (goat anti-mouse
1
polyclonal antibody conjugated to
horseradish peroxidase, or HRP),
lyophilized
HRP color detection reagent A
1
HRP color detection reagent B
1
10x Tris/Glycine, 1 L
1
Nonfat dry milk blocker
1
10x Phosphate buffered saline (PBS), 100 ml 2
10% Tween 20, 5 ml
1
Nitrocellulose, 0.45 µm
8 sheets
Blotting paper
16 sheets
Reagent tubes
25
Curriculum, including teacher’s guide,
1
student manual, and graphic quick guide
Required Accessories Not Included in Kit:
Comparative proteomics kit I: protein
1
profiler module, p. 38
Mini-PROTEAN TGX precast polyacrylamide 8
gels, 4–20%, 10-well each, p. 123
Vertical gel electrophoresis chambers, p. 120 4
Adjustable micropipet, 2–20 µl, p. 138
4–8
Power supplies, p. 130
2–4
Water bath or dry bath, pp. 136–137
1
Rocking platform, pp. 135–136
1
Reagent alcohol or ethanol, 0.8–2 L
Fish samples
5–8 types
Gel staining trays, p. 143
4–8
Recommended (Optional) Accessories:
Electroblotter, p. 122
Labs 1 & 2
Lab 3
4
Refresh Kit Components: (more info pp. 84, 143)
Western blot temperature-sensitive $128.00
reagent refill pack (#166-2801EDU)
includes primary antibody,
secondary antibody, HRP color
detection reagents A and B
Primary antibody (anti-myosin light
56.00
chain antibody), 200 µg, lyophilized
(#166-2804EDU)
Secondary antibody (goat anti-mouse 124.00
HRP), 2 ml (#172-1011EDU)
Horseradish peroxidase (HRP)
80.80
conjugate substrate kit
(#170-6431EDU)
Cell culture tubes, 17 x 100 mm, 14 ml, 17.00
sterile, 25 (#166-0476EDU)
Gel staining trays, 4 (#166-0477EDU)
16.00
Racks, p.142
Lab 4
Protein profiler temperature-sensitive
reagent refill pack, p. 39
Nitrocellulose, 0.45 µm, 10 sheets, p. 125
Nitrocellulose/filter paper packs, p. 125
Thick blot paper, 50 sheets, p. 125
Blotting-grade blocker, nonfat dry milk,
300 g, p. 125
Laemmli sample buffer, p. 125
Precision Plus Protein™ Kaleidoscope™
standards, p. 126
Actin and myosin standard, p. 39
10x Tris/glycine/SDS, p. 125
10x Tris/glycine, p. 125
10x Phosphate buffered saline (PBS), p. 125
10% Tween 20, p. 125
Bio-Safe™ Coomassie stain, p. 127
Visit us on the Web at explorer.bio-rad.com
41
New Rapid Blotting — V3 Western Workflow™: Stain-Free Rapid Blotting
Rapid Blotting — V3 Western Workflow
Each kit contains sufficient materials for 8
student workstations.
Rapid Blotting + V3 Western Workflow
Starter Kit
Catalog #
List Price . . . . . . . . .EDU Price
Includes Protein Profiler and Western Blot modules;
Trans-Blot Turbo mini nitrocellulose transfer pack, 10
pack; TGX Stain-Free precast gels, 4–20%, 10 pack;
Rapid Blotting + V3 Western Workflow Application Note.
Hands-on time expenditure, in min
Protein extraction
Electrophoresis
Protein visualization
Protein transfer
Equilibration
Setup
Transfer
Immunoblotting
Color detection
Total hands-on time
Rapid blotting
(staining required)
15
18
180
15
18
180
15
18
<3
15
30
30–150
45
0
5
15
45
all 10 min–overnight
0
5
15
45
343–463
(5 hrs 43 min –
7 hrs 25 min)
288
(4 hrs 48 min)
Obtain fish samples locally. Mini-PROTEAN® TGX
Stain-Free™ gels require a UV imager to visualize the
resolved protein samples. Traditional SDS-PAGE
gels can be used in place of Mini-PROTEAN TGX
Stain-Free gels, but require staining/destaining of
gels to visualize the resolved protein samples.
V3 Western Workflow
(stain-free rapid blotting)
Key Kit Features
• Explore immunodetection
• Apply immunology
• Use antibodies as tools
• Understand how protein variation supports
evolutionary relatedness
• Compare information provided by stained
gels vs. immunoblots
• Complete in less than 2 hours
111
(1 hr 51 min)
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Total Time Savings of 55 min to 7 1/2 hrs
Stain-free imaging of total fish protein samples in the
gel before and after protein transfer, and on the
nitrocellulose membrane after protein transfer.
Gel after
Proteins are visible on the gel prior to
transfer and are visible on the
membrane after transfer.
Gel before
•
•
•
•
Use of immunodetection to identify specific proteins
Use of experimental controls
Creation and use of standard curves
Use of bioinformatics databases
• Post-transcriptional and
post-translational modification
• Muscle protein structure
and function
• Adaptations to environment
• Immunology and virology
Membrane
42
1-800-4BIORAD (1-800-424-6723)
• Molecular variation
• Evolution of adaptive traits
• Environment and natural
selection
• Phylogenetic relationships
Required Accessories Not Included in Kit:
• Vertical gel electrophoresis
8
chambers, p. 120
• Power supplies, p. 130
2–4
• Dry bath, p. 136
1
• Rocking platform, pp. 135-136
1
• Adjustable micropipets, 2–20 µl,
4–8
p. 138
• Trans-Blot Turbo transfer system
1–4
(170-4150EDU)
• Fish samples
5–8 types
• Gel staining trays, p. 143
4–8
Recommended (Optional) Accessories:
• Gel Doc™ EZ imaging system with white
light sample tray (170-8270EDU)
• Sample tray holder (170-8276EDU)
• Pipet controller (166-0490EDU)
• Gel cutter (170-3760EDU) or
Gel releasers (165-3320EDU)
• Blot roller (165-1279EDU)
Refresh Kit Components: (more info pp. 84, 143)
Kit I: Protein Profiler module
$175.00
(166-2700EDU)
Kit II: Western Blot module
199.00
(166-2800EDU)
Trans-Blot Turbo mini nitrocellulose
114.40
transfer pack, 10 pack
(170-4158EDU) or
Trans-Blot Turbo midi nitrocellulose
139.20
transfer packs, 10 pack
(170-4159EDU)
TGX Stain-Free precast gels, 4–20%,
91.20
10 pack (456-8093EDU)
Science and
Engineering
Practices
V3
Western
Workflow
Cellular
Proccesses
Evolution
• Comparative Proteomics Kits I and II:
Protein Profiler and Western Blot modules
(166-2850EDU)
• Trans-Blot Turbo mini nitrocellulose transfer
pack, 10 pack
• Mini-PROTEAN® TGX™ or TGX Stain-Free
4–20% precast polyacrylamide gels, 10 pack
• Rapid Blotting + V3 Western Workflow
application note
Lab 1
Ecosystems,
Environment,
and
Interactions
• Ecosystems, symbiosis,
and interdependence
• Real-world HIV, mad cow
disease, and virus testing
Lab Preparation Checklist
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
166-2875EDU* $700.00 . . . . . . . .$560.00
Tank
blotting
CLASSROOM KITS: RAPID BLOTTING — V3 WESTERN WORKFLOW: STAIN-FREE RAPID BLOTTING
Western blotting in less than 2 hours! Using our new rapid blotting or
V3 Western Workflow (stain-free rapid blotting) options allows you to complete
the entire western blot workflow in less than 2 to 5 hours, depending on which
time-saving steps you incorporate. TGX Stain-Free™ gels combined with our superfast Trans-Blot® Turbo™ transfer system provide the fastest speed and most time
savings — allowing you to complete the workflow in less than a single 3 hour lab
block. Teach your students about the exciting new chemistry that allows visualization
of samples separated on PAGE gels without staining! To learn more, visit us at
explorer.bio-rad.com to download the Rapid Blotting + V3 Western Workflow
application note, which shows you how to perform the Comparative Proteomics kits I
and II: Protein Profiler and Western Blot modules in less than 2 hours. This same
workflow can provide time savings for any western blotting application. See for
yourself why thousands of researchers are using this new process!
Molecules to
Organisms
Genetics and
Heredity
• Chemical and physical
properties of proteins
• Antibody structure and function
• Protein extraction techniques
• Enzyme-substrate interactions
• Chemistry of electrophoresis
and blotting
• DNA > RNA > Protein > Trait
• Antibody production via genetic
recombination and gene splicing
• Genetic and epigenetic factors
Visit us on the Web at explorer.bio-rad.com
43
DNA Analysis and Agarose Gel Electrophoresis Kits
Section Contents
Genes in a Bottle™ Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
New IDEA and STEM Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Forensic DNA Fingerprinting Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Lambda DNA Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CLASSROOM KITS: DNA ANALYSIS KITS
DNA Analysis and Agarose Gel Electrophoresis Kits
“
Bio-Rad products
have made learning
biotechnology fun and
accessible to students.
Without Bio-Rad I
wouldn’t have the
time to put together
my own kits.
”
1>
Adrienne Fong
Hiram Johnson High School
Sacramento, California
3>
2>
44
4>
1-800-4BIORAD (1-800-424-6723)
5>
1. Leander High School, Leander, Texas; 2. Bio-Rad Teacher Training, Hong Kong,
China; 3. Germantown Friends School, Philadelphia, Pennsylvania; 4. Chula Vista
High School, Chula Vista, California; 5. University of Florida, Alachua, Florida.
45
New and Improved Genes in a Bottle™ Kit: Make Biology Personal
Genes in a Bottle Kit
Lab Preparation Checklist
Kit contains sufficient materials for 9 student
workstations (2–4 students per workstation).
For students learning about the molecular framework of biology for the first time, DNA is
abstract and intangible. This procedure makes the invisible visible — seeing their own
DNA makes it real and helps students comprehend this primary substance of life. From cell
structure to genetics to the chemistry of life, this kit integrates multiple life science standards
in a single lesson. Seeing DNA makes it real. Wearing it makes the lesson memorable!
Each kit supports 36 students.
Genes in a Bottle Kit
Catalog #
List Price . . . . . .EDU Price
166-2300EDU*
$167.50 . . . . .$134.00
Ships and stores at room temperature.
* Includes 1 DNA extraction module and 1 DNA
necklace module.
How do scientists separate pure DNA from cells composed mainly of lipids, proteins,
carbohydrates, and salts? Membranes are first ruptured with detergent to release DNA into
a solution, then proteins and other organic molecules are digested and separated while
retaining intact DNA. The DNA is finally collected by precipitation in a form that can be
manipulated as desired.
166-2000EDU
118.75 . . . . . . .95.00
DNA Extraction Module
166-2250EDU
82.50 . . . . . . .66.00
Helix DNA Necklace Module (36 necklaces)
With this simple lab activity, your students will extract genomic DNA from their own cheek
cells and watch it precipitate from solution as floating white strands. The DNA strands
are then easily collected and transferred to a helix keepsake vial, and the vial is fashioned
into a necklace!
Learning opportunities for all levels of instruction. This activity is designed for any
classroom environment and requires no specialized equipment or stains. For secondary and
college level instruction, lessons on DNA structure and function, cell structure, and enzyme
function can be introduced or reinforced with this laboratory activity. For middle school
students, it’s a perfect introduction to the exciting world of DNA science.
Key Kit Features
• Perform real research techniques
• Collect cheek cells
• Extract, precipitate, and bottle your DNA
• Use as introductory or capstone activity
• Complete in one 45 minute lab session
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
• Conduct human genomic
DNA extraction procedure
• Extract DNA from cheek cells
• Precipitate and preserve DNA
DNA Helix Keepsake
with
New Screw Caps
Ecosystems,
Environment,
and
Interactions
• Genetic testing
• DNA profiling
• Forensic DNA science
• Eukaryotic cell structure and
organization
• Organelles and cell membranes
• Cell nucleus and DNA staining
CLASSROOM KITS: GENES IN A BOTTLE KIT
Seeing is believing. Introduce your students to molecular biology with their own DNA!
Enable your students to see the normally invisible substance of life and begin to comprehend
the meaning of their own genetic makeup. In this activity your students employ the same
real-world laboratory procedure used to extract DNA from many different organisms for a
variety of biotechnology research applications. Students extract genomic DNA from their
own cheek cells, then precipitate and bottle it in a fabulously cool helix keepsake.
DNA Necklace Module
Helix keepsake vials
Silver screw caps
Waxed string
36
36
36
Required Accessories Not Included in Kit:
91% isopropyl alcohol or 95% ethanol,
1
360 ml
Recommended (Optional) Accessories:
Water bath or dry bath with thermometer,
1
p. 136–137
Rack to hold 15 ml tubes in water bath
1
(need space for 36 tubes) (#166-0483EDU)
Refresh Kit Components: (more info pp. 84, 143)
Genes in a Bottle DNA extraction
$40.00
reagent refill pack (#166-2001EDU)
includes lysis buffer and powdered
protease + salt
Lysis buffer, 100 ml (#166-2002EDU)
Conical centrifuge tubes, 15 ml, 50
(#166-0475EDU)
Disposable plastic transfer pipets,
nonsterile, 500 (#166-0480EDU)
Green racks, set of 5 racks
(#166-0481EDU)
15 ml tube racks, holds 60 tubes,
set of 5 racks (#166-0483EDU)
23.00
26.00
52.00
50.01
70.20
I Love Your DNA Tattoos,
200 temporary tattoos
(#166-2004EDU)
24.00
DNA Model, p. 117
(#166-7015EDU)
24.99
Science and
Engineering
Practices
Genes
in a
Bottle Kit
Cellular
Proccesses
1 DNA Extraction Module
Lysis buffer, 100 ml
1
Powdered protease + salt, 1.5 g
1
Conical tubes, 15 ml
50
Multicolor microcentrifuge tubes
60
Disposable plastic transfer pipets
60
Curriculum, including teacher’s guide,
1
student manual, and graphic quick guide
Molecules to
Organisms
Genetics and
Heredity
• Chemical and physical
properties of DNA
• Properties of enzymes
• Solubility of biological
molecules
Genes in a Bottle Kit Fundraising Activity
• DNA and genetic
variation among individuals
• Genetic inheritance
46
1-800-4BIORAD (1-800-424-6723)
Evolution
•
•
•
•
DNA location, structure, and function
Genes, chromosomes, and genomes
Chromosomal inheritance
Human genome structure
See how to raise funds for your science class
with this fun activity. More on the Web at
explorer.bio-rad.com.
Lab 1
Visit us on the Web at explorer.bio-rad.com
47
New IDEA — Inquiry Dye Electrophoresis Activity and STEM Electrophoresis Kits
IDEA and STEM Electrophoresis Kits
The number of workstations vary depending
on the kit purchased. Please refer to the
chart for information regarding the number
of workstations served.
IDEA Kit Starter Pack supports 8 student workstations.
Engineer the tools for biological discovery. What actually happens in an agarose
gel electrophoresis chamber? Reveal the secrets of this “black box” with Bio-Rad’s
STEM Electrophoresis Kit. Give your students the opportunity to learn about critical
design aspects of an electrophoresis unit by engineering one! This activity addresses the
fundamentals of science, technology, engineering, and math (STEM) with an integrated
hands-on approach. This gel electrophoresis unit is designed to run the IDEA kit, which
will bring engagement and an additional inquiry component into your classroom.
STEM Electrophoresis Kit Starter Pack supports
8 student workstations.
Ordering Information
100 ml
50x TAE
and 5 g
Agarose
IDEA Kit
Reagent
Refill*
10 µl Fixed
Volume
Micropipets
STEM
Electrophoresis
Engineering
Module**
Printed
Manual
Number of
Workstations
Catalog #
IDEA Kit
IDEA Kit Reagent Refill Pack
IDEA Kit Starter Pack
yes
—
yes
yes
yes
yes
—
—
8
—
—
—
IDEA
—
IDEA
8
8
8
166-5075EDU
166-5076EDU
166-5077EDU
STEM Electrophoresis
Demonstration Kit
STEM Electrophoresis
STEM Electrophoresis
STEM Electrophoresis
Starter Pack
Teacher
yes
yes
—
1
STEM
2
166-5080EDU
Kit
Classroom Kit
Kit
—
yes
yes
—
yes
yes
—
—
8
1
4
4
STEM
STEM
STEM
2
8
8
166-5085EDU
166-5090EDU
166-5095EDU
Description
Each kit ships and stores at room temperature.
•
•
•
•
Use of sophisticated laboratory techniques
Evaluation of hypothesis
Interpretation of experimental results
Independent student research projects
Ecosystems,
Environment,
and
Interactions
Science and
Engineering
Practices
Required Accessories Not Included in
IDEA Kit:
Horizontal gel electrophoresis chambers, 4–8
p. 104 or STEM kit
Adjustable micropipets, p. 138
2–20 µl or 10 µl fixed volume
1–8
20–200 µl and 100–1,000 µl
1 each
Pipet tips, p. 140
Power supplies, p.130
2–4
4
2
2
2
Required Accessories Not Included in
STEM Electrophoresis Kit:
9 V Batteries
3–5 per workstation
2–20 µl adjustable or
10 µl fixed-volume pipets, p. 138
1–8
2–200 µl pipet tips, p. 140
Plastic rulers or plastic card to cut gels
8
Eye droppers
8
Refresh Kit Components: (more info pp. 84, 143)
IDEA kit
STEM Electrophoresis kit
LAB 1
IDEA Kit Reagent Refill Pack
(166-5076EDU) includes blue 1
reference dye, yellow 5 reference dye,
yellow 6 reference dye, red 40 reference
dye, dye extraction solution, and 72
microcentrifuge tubes
2 ml microcentrifuge tubes, clear, 500
(223-9430EDU)
STEM
and IDEA
Kits
LAB 2
Molecules to
Organisms
• Gel electrophoresis
Cellular
Proccesses
IDEA Kit (166-5075EDU)
Dye extraction solution, 25 ml
1
Blue 1 reference dye, 150 µl
1
Yellow 5 reference dye, 150 µl
1
Yellow 6 reference dye, 150 µl
1
Red 40 reference dye, 150 µl
1
Electrophoresis buffer, 50x TAE, 100 ml
1
Molecular biology grade agarose, 5 g
1
2 ml microcentrifuge tubes
72
Curriculum, including teacher’s guide,
1
student manual, and graphic quick guide
STEM Electrophoresis Kit (166-5085EDU)
Paper clips
Black alligator clip leads
Red alligator clips leads
8-well combs
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
* Includes 4 reference dyes, dye extraction solution, and microcentrifuge tubes (166-5076EDU).
** Includes red and black alligator clips, 8-well combs, paperclips, and plastic hinged box, 2 workstations
per module (166-5085EDU).
• Biodiversity
• Role, place, limits, and
possibilities of science
and technology
• Nutrition
• Populations, ecosystems,
and human impacts
Lab Preparation Checklist
CLASSROOM KITS: IDEA AND STEM ELECTROPHORESIS KITS
Electrophoresis is a fundamental skill used daily in the molecular biology
laboratory. Bridge the gap between textbook science and students’ lives by using dyes
extracted from candy coatings to perform agarose gel electrophoresis. Bio-Rad’s IDEA Kit
is a dazzling way for students to learn the basics of this key technique using dyes that are
commonly found in the foods they eat. Combine this with the power of inquiry to encourage
your students to ask questions and seek answers. What dye combinations create the colors
in the hard-shell candies of their choice? Do red and blue make purple? Is pink really pink?
The colorful results may surprise you and will certainly get your students talking about
their discoveries.
Genetics and
Heredity
• Coupled reactions and
free energy changes
• Chemical properties
of molecules
• Buffers, solubilities,
and pH
• Food allergies
• Perception of color
Evolution
48
Visit us on the Web at explorer.bio-rad.com
49
Forensic DNA Fingerprinting Kit — AP Big Idea 3: Who Done It?
Forensic DNA Fingerprinting Kit
Each kit supports 32 students.
Forensic DNA Fingerprinting Kit
Catalog #
List Price . . . . . . . . .EDU Price
166-0007EDU
$166.25 . . . . . . . .$133.00
We’ve gone green! The curriculum manual is no
longer included in this kit. It is available to download
free of charge online.
Convenient lyophilized reagents. Ships at room
temperature. Store reagents bag at –20°C.
In this lab, students observe the effects of two DNA restriction enzymes on a series of
plasmid DNA samples. The six DNA samples in this kit are plasmids engineered to mimic the
natural variations in DNA that exist between one human being and another. One DNA sample
has been collected from a “crime scene” and five samples have been obtained from various
“suspects.” Each sample is digested using a mixture of two DNA restriction enzymes, generating
a distinct set of DNA fragments for each sample. The resulting DNA fragments are separated by
agarose gel electrophoresis and visualized using Bio-Rad’s revolutionary Fast Blast™ DNA stain.
Forensic DNA Fingerprinting Kit with
printed curriculum manual
166-0037EDU
173.75 . . . . . . . . .139.00
Key Kit Features
• Aligns with AP Biology Big Idea 3; Lab 9
• Study DNA and restriction enzyme functions
• Use electrophoresis to visualize DNA fragments
• Construct standard curves from student data
• Make precise determinations of DNA
fragment sizes
• Complete in two 45 minute lab sessions
This activity provides in-depth explanations about how restriction enzymes cut DNA and how
electrophoresis is used to separate and visualize DNA fragments. The unique curriculum provided
in this kit guides students through the procedure of constructing a standard curve using their
own gel data. They can then use their standard curve to estimate the molecular weights of the
unknown DNA fragments generated by different restriction enzymes.
Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and
in the mapping of restriction sites within genes. With the curriculum in this kit, students also have
the opportunity to read plasmid maps and predict the sizes of DNA fragments from restriction
enzyme digests prior to performing the lab. They can go one step further and use restriction
digest maps of lambda bacteriophage genomes (provided in the kit curriculum) to design novel
plasmids. In the process of doing these extension activities, students learn how restriction
enzymes function and how they are used in genetic engineering.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Use this kit to open the door to rich discussions about the scientific, ethical, and legal
implications of forensics, DNA profiling, and genetic engineering.
• Use of restriction enzymes and
electrophoresis to fingerprint DNA
• Use of experimental controls
• Interpretation of experimental results
• Use of DNA evidence in court
• Creation and use of standard curves
7 x 10 cm ReadyAgarose™ gel runs
in the Mini-Sub® cell GT cell
(see page 104)
Ecosystems,
Environment,
and
Interactions
Science and
Engineering
Practices
Forensic
DNA
Fingerprinting
Molecules to
Organisms
Kit
• Cell structure and organization
• Tissue types for biological
sampling
• Plasmid mapping
• Genetic variations in the
human genome
• Genotype vs. phenotype
• Biodiversity
• Bacterial defense
mechanisms
50
1-800-4BIORAD (1-800-424-6723)
Cellular
Proccesses
Evolution
Lab Preparation Checklist
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
Restriction enzymes are essential tools for molecular cloning and the mapping of genes and
genomes. They are also used in genetic engineering to create recombinant DNA molecules for
transforming bacterial, plant, or animal cells. Restriction enzymes recognize specific doublestranded DNA sequences and they cut the DNA by making two incisions, one through each of
the phosphate backbones of the double helix. The chemical bonds that the enzymes cleave are
easily reformed by DNA ligases, so that restriction fragments carved from the DNA of different
organisms can be spliced together, creating new hybrid organisms.
• Epidemiology and disease
• Genetic testing
• Role, place, limits, and
possibilities of science and
technology
• Privacy of information issues
CLASSROOM KITS: FORENSIC DNA FINGERPRINTING KIT — AP BIG IDEA 3
Using DNA as evidence, students figure out for themselves, “Who done it?”
DNA evidence assists in criminal, missing person, mass disaster, and paternity cases. It can
be used to identify a perpetrator or exonerate the innocent. Using real DNA as evidence,
your students play the role of crime scene investigator.
Genetics and
Heredity
DNA size standard
1
(HindIII lambda DNA digest), 100 µl
DNA samples, lyophilized, 60 µg each
Crime scene sample
1
Suspect samples
5
EcoRI/PstI restriction enzyme mix,
1
lyophilized, 3,000 units
Sample loading buffer, 5x, 1 ml
1
Electrophoresis buffer, 50x TAE, 100 ml
1
Sterile water, 2.5 ml
1
Agarose powder, 5 g
1
Fast Blast DNA stain, 500x, 100 ml
1
Colored microcentrifuge tubes, 2.0 ml
60
Microcentrifuge tubes, 1.5 ml
30
Foam floats
8
We’ve gone green! The curriculum is available
to download free online or for a small fee
when ordering with the kit (#166-0037EDU).
Required Accessories Not Included in Kit:
Horizontal gel electrophoresis
4–8
chambers, p. 104
Adjustable micropipets, p. 138
2–20 µl
1–8
20–200 µl and 100–1,000 µl
1 each
Pipet tips, p. 140
2–1,000 µl, BR-35, 40
1 bag each
Power supplies, p. 130
2–4
Gel staining trays, p. 143
4–8
Lab 1
Recommended (Optional) Accessories:
Water bath or dry bath, pp. 136–137
Mini centrifuge, p. 134
Rocking platform, pp. 135–136
Gel support film, p. 106
Microwave oven
Refresh Kit Components: (more info pp. 84, 143)
DNA fingerprinting kit reagent refill
$120.00
pack (#166-0027EDU) includes crime
scene + suspect DNA samples, EcoRI/PstI
restriction enzyme mix, sample loading buffer,
DNA size standard, sterile water
EcoRI/PstI restriction enzyme mix,
46.00
lyophilized, 3,000 units (#166-0047EDU)
Fast Blast DNA stain (#166-0420EDU) 24.00
UView™ 6x loading dye, p. 109
Gel staining trays, 4 (#166-0477EDU)
16.00
Jellyfish foam floating racks, 8 racks
30.00
(#166-0479EDU)
Curriculum manual, printed (#166-0077EDU) 6.00
DNA electrophoresis reagent packs, p. 106
Lab 2
See Bulletin 5396 at explorer.bio-rad.com.
• DNA structure, function,
and chemistry
• Chemistry of DNA
electrophoresis
• Biochemistry of
restriction enzymes
• Restriction fragment length
polymorphism (RFLP analysis)
• Genetic engineering techniques
• Mendelian inheritance
• Plasmid mapping
Forensic DNA Fingerprinting Curriculum
Download the Get a Clue curriculum that
embeds the forensic DNA fingerprinting lab in
topical case study scenario format, developed
by the University of North Carolina, Chapel Hill
DESTINY Traveling Science Learning Program.
Download free at explorer.bio-rad.com.
Visit us on the Web at explorer.bio-rad.com
51
Lambda DNA Kits — AP Big Idea 3: How’s This for a Slice of Life?
Lambda DNA Kits
Each kit supports 32 students.
Analysis of Precut Lambda DNA Kit
Key Kit Features
• Aligns with AP Biology Big Idea 3; Lab 9
• Study DNA restriction enzyme function
• Use electrophoresis to separate DNA fragments
• Construct standard curves from student data
• Make precise determinations of DNA
fragment sizes
• Complete in one to two 45 minute lab sessions
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Lambda bacteriophage has been a workhorse of molecular biology for decades. It is vital in
the fields of molecular cloning and genomic sequencing since it can be used to subclone very long
genomic DNA fragments much more efficiently than plasmid vectors. Lambda DNA comes from a
bacterial virus, or bacteriophage, which attacks bacteria by injecting them with its nucleic acid.
Once inside, lambda DNA hijacks the bacterial cellular machinery and replicates itself until the
cells burst, releasing millions more bacteriophages to carry out the same infection process.
Bacteriophage lambda is harmless to humans and other eukaroytic organisms and
therefore makes an excellent source of DNA for experimental study.
• Use of restriction enzymes and DNA
gel electrophoresis
• Use of experimental controls
• Interpretation of experimental results
• Creation and use of standard curves
Ecosystems,
Environment,
and
Interactions
• Prokaryotic cell
structure and function
• Virus structure and
function
1-800-4BIORAD (1-800-424-6723)
$118.75 . . . . . . . . .$95.00
Restriction enzymes ship on blue ice; store in
freezer (–20°C).
Banding patterns from each sample are then compared to each other and to a DNA size
standard. From their electrophoresis results, students construct standard curves and determine
the precise DNA fragment sizes generated by the different restriction enzymes. By visualizing
the effects of different enzymes on identical samples of double-stranded DNA, students learn
that different restriction enzymes recognize and cut different DNA sequences.
52
List Price . . . . . . . . .EDU Price
166-0001EDU
Restriction Digestion and Analysis of
Lambda DNA Kit
166-0002EDU
133.75 . . . . . . . . .107.00
Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics
and in the mapping of restriction sites within genes. The restriction digestion and analysis of
lambda DNA kit and the analysis of precut lambda DNA kit each provide in-depth explanations
about how restriction enzymes cut DNA and how electrophoresis can be used to separate and
visualize DNA fragments.
• Bacterial defenses
against viral infection
• Genetic diversity
Catalog #
Ships at room temperature; store at 4°C.
The analysis of precut lambda DNA kit demonstrates the principles, results, and analysis
of restriction digestion without the extra time needed to perform the digestion.
• Virology and
microbiology
• Epidemiology and
disease
Science and
Engineering
Practices
Lambda
DNA Kits
Cellular
Proccesses
Evolution
Lab Preparation Checklist
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
The restriction digestion and analysis of lambda DNA kit uses three different
restriction enzymes to digest genomic samples of the lambda bacteriophage. The lambda
genome has approximately 48,000 base pairs. Each restriction enzyme will cut the lambda
DNA several times, generating distinct sets of DNA restriction fragments of different sizes. The
three different sets of DNA fragments that result are separated by agarose gel electrophoresis
and visualized using Bio-Rad’s safe Fast Blast™ DNA stain.
7 x 10 cm ReadyAgarose™ gel runs in the
Mini-Sub® cell GT cell (see page 104).
Analysis of precut lambda DNA kit
results seen on the top half of the gel.
Restriction digestion and analysis of
lambda DNA kit results seen on
bottom half of the gel.
CLASSROOM KITS: LAMBDA DNA KITS — AP BIG IDEA 3
Restriction enzymes are essential tools for genetic engineering, gene mapping, and
genome sequencing. Restriction enzymes recognize specific double-stranded DNA sequences
and they cut the DNA by making two incisions, one through each of the phosphate backbones of
the double helix. The chemical bonds that restriction enzymes cleave are easily reformed such
that DNA fragments carved from a virus, bacteria, plant, or animal can be inserted (subcloned)
into vectors such as plasmid DNA or lambda DNA, creating recombinant DNA molecules that
can be sequenced (or transformed back into bacterial, animal, and plant cells) creating hybrid
organisms with new genetic traits to study.
Molecules to
Organisms
Genetics and
Heredity
• DNA structure, function,
and chemistry
• Biochemistry of restriction
enzymes
• Chemical properties of
biological molecules
• Chemistry of DNA
electrophoresis
• Lambda bacteriophage life cycle
• Restriction enzymes as genetic
engineering tools
• DNA > RNA > Protein > Trait
Analysis of Precut Lambda DNA kit contains:
PstI lambda DNA digest
1
EcoRI lambda DNA digest
1
Restriction Digestion and Analysis of
Lambda DNA kit contains:
HindIII restriction enzyme
1
PstI restriction enzyme
1
EcoRI restriction enzyme
1
Restriction buffer
1
Foam floats
8
Both lambda DNA kits contain:
Lambda DNA, uncut
1
DNA size standard
1
(HindlII lambda DNA digest)
Sample loading buffer, 5x, 1 ml
1
Agarose powder, 5 g
1
Electrophoresis buffer, 50x TAE, 100 ml
1
Fast Blast DNA stain, 500x, 100 ml
1
Colored microcentrifuge tubes, 2.0 ml
60
Curriculum, including teacher’s guide,
1
student manual, and graphic quick guide
Required Accessories Not Included in Kit:
Horizontal gel electrophoresis
4–8
chambers, p. 104
Adjustable micropipets, p. 138
2–20 µl
4–8
20–200 µl
1
Pipet tips, p. 140
1 bag
2–200 µl, BR-35
Power supplies, p. 130
2–4
Gel staining trays, p. 143
4–8
Recommended (Optional) Accessories:
Water bath or dry bath (for Restriction
Digestion and Analysis of Lambda
DNA kit), pp. 136–137
Rocking platform, pp. 136–137
Gel support film, p. 106
Microwave oven
Refresh Kit Components: (more info pp. 84, 143)
Restriction digestion kit TS reagent
$91.00
refill pack (#166-0012EDU) includes
HindIII, PstI, and EcoRI restriction enzymes,
restriction buffer, uncut lambda DNA, DNA
size standard, sample loading buffer
Restriction digestion kit RT reagent
48.00
refill pack (#166-0022EDU) includes
agarose powder, electrophoresis buffer,
Fast Blast DNA stain, microcentrifuge
tubes, foam floats, curriculum
Precut lambda DNA kit reagent refill
77.00
pack (#166-0011EDU) includes DNA size
standard, PstI lambda DNA digest, EcoRI
lambda DNA digest, uncut lambda DNA,
sample loading buffer
Fast Blast DNA stain (#166-0420EDU)
UView™ 6x loading dye, p.109
Gel staining trays, 4 (#166-0477EDU)
Jellyfish foam floating racks, 8 racks
(#166-0479EDU)
Lab 1
24.00
16.00
30.00
DNA electrophoresis reagent packs, p. 106
See Bulletin 5396 at explorer.bio-rad.com.
Visit us on the Web at explorer.bio-rad.com
53
Classroom Kits: PCR Amplification Kits
Section Contents
Crime Scene Investigator PCR Basics™ Kit . . . . . . . . . . . . . . . . . . . . . . . . . 56
PV92 PCR Informatics Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
New Fish DNA Barcoding Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
GMO Investigator™ Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Real-Time PCR Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
“
Bio-Rad developed a
fantastic workshop for our
local area in-service and
pre-service teachers. Not
only did participants learn
new content and exciting
ways to incorporate
biotechnology into their
curriculum, they got
a primer on grant writing
and ideas for funding
sources. It was a great
6 hours of professional
development.
5>
CLASSROOM KITS: PCR AMPLIFICATION KITS
PCR Amplification Kits
”
Laura Henriques, PhD
Science Education Faculty
Faculty Advisor for CSTA/NSTA Student Chapter at CSULB
California State University Long Beach
Long Beach, California
1>
4>
2>
54
1-800-4BIORAD (1-800-424-6723)
3>
6>
1. Santa Fe College, Alachua, Florida; 2. University of San Francisco,
San Francisco, California; 3. North Carolina A&T, Greensboro, North Carolina;
4. Sacred Heart High School, San Francisco, California; 5. University of Texas,
Corpus Christi, Texas; 6. BEANZ BioLive Conference, Auckland, New Zealand.
55
Crime Scene Investigator PCR Basics™ Kit: How Does DNA Solve Crimes?
Crime Scene Investigator PCR Basics Kit
Each kit supports 32 students.
What kinds of human DNA sequences are used in crime scene investigations?
There are ~3 billion bases in the human genetic blueprint, and more than 99.5% of them do not
vary among human beings. Within the variant areas of the genome are the special polymorphic
(“many forms”) sequences used in forensic applications. The DNA sequences used for forensic
typing are derived from regions of our chromosomes that do not control any known traits and
have no known functions. They contain segments of short tandem repeats, called STRs. STRs
are very short DNA sequences that are repeated in direct head-to-tail fashion. The example
below shows a locus (known as TH01) actually used in forensic DNA profiling. Its specific DNA
sequence contains five repeats of [TCAT].
Crime Scene Investigator PCR Basics Kit
Suspect B’s DNA type for TH01 locus is (6–10)
CCC
3*
CCC
CCC
AAA
AAA
To pour, run, and stain forty-eight 1% or sixteen 3%
7 x 10 cm agarose gels
Crime Scene Investigator PCR Basics Kit
Plus Small Fast Blast DNA Electrophoresis
Reagent Pack
166-2650EDU
270.00 . . . . . . . . .216.00
6*
AAA
$217.50 . . . . . . . .$174.00
Small Fast Blast™ DNA
Electrophoresis Reagent Pack
166-0450EDU
105.00 . . . . . . . . . .55.00
Two sample TH01 genotypes
5*
List Price . . . . . . . . .EDU Price
166-2600EDU
Store reagents bag at –20°C.
Electrophoresis reagents not
included — available separately.
For the TH01 STR locus, there are many alternate forms (alleles) that differ from each other by
the number of [TCAT] repeats present in the sequence. More than 20 different alleles of TH01
have been discovered in people worldwide. Each of us still has only two alleles, one inherited
from our mother and one inherited from our father.
AAA
Catalog #
Ships at room temperature.
...CCC TCAT TCAT TCAT TCAT TCAT AAA...
CCC
Key Kit Features
• Perform real-world DNA profiling
• Use PCR to amplify multiple DNA samples
• Use electrophoresis to visualize results
• Complete in two 45 minute lab sessions
10*
* Number of [TCAT] repeats
How are STR alleles detected? Each STR allele has a different length depending on the
number of tandem repeats it contains. When the alleles are amplified by PCR, alleles of different
lengths can be distinguished by electrophoresis. The number of tandem repeats contained in
each allele can be determined by comparing the locations of the DNA bands with a DNA size
standard that corresponds to the known sizes of TH01 alleles.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
The DNA samples contained in this kit are plasmids that have been engineered to mimic the natural
variations in human DNA that exist between one human being and another at a single STR locus. In
real crime scene applications, using the international Combined DNA Index System (CODIS), DNA
profiling is performed using 13 loci to increase the power of discrimination. Extension exercises in
the kit curriculum provide access to real profiling data, enabling students to perform statistical
analyses and apply the power of discrimination. With the addition of each locus to the analysis,
the possibility that any two genotypes will match due to chance drops off significantly.
This exercise allows students to grasp the concept of the power of discrimination —
the more loci that are used, the finer the discrimination between any two samples.
Ecosystems,
• Use of PCR and gel electrophoresis
for DNA profiling
• Use of experimental controls
• Interpretation of experimental results
• Use of forensic evidence in the courts
Science and
Engineering
Practices
Environment,
and
Interactions
•
•
•
•
Population genetics
Genetic screening
Genetic databases
Role, place, limits of
science and technology
• Privacy of information issues
Crime Scene
Investigator
PCR Basics
Molecules to
Organisms
Kit
• Eukaryotic cell structure
and organization
• Tissue types and biological
sampling
• Genetic diversity and
individual identification
• Allele frequencies in
populations
• Statistics, probabilities, and
the power of discrimination
56
1-800-4BIORAD (1-800-424-6723)
Cellular
Proccesses
Evolution
Lab Preparation Checklist
Kit (166-2600EDU) contains sufficient
materials for 8 student workstations
(2–4 students per workstation).
DNA profiling determines the exact genotype of a DNA sample and distinguishes one
human being from another by identifying a DNA “barcode” that is unique to every individual.
This powerful tool assists in investigations of crime scenes, missing persons, mass disasters,
immigration disputes, and paternity testing.
Suspect A’s DNA type for the TH01 locus is (5–3)
CLASSROOM KITS: CRIME SCENE INVESTIGATOR PCR BASICS KIT
This introductory PCR kit allows students to simulate DNA profiling as commonly used in
forensic labs. The lab is designed to introduce the concepts of PCR to students in two lesson
periods without the need for complex genomic DNA extraction steps.
Genetics and
Heredity
•
•
•
•
•
•
Crime scene DNA sample, 250 µl
1
Suspect A DNA sample, 250 µl
1
Suspect B DNA sample, 250 µl
1
Suspect C DNA sample, 250 µl
1
Suspect D DNA sample, 250 µl
1
PCR master mix (Taq DNA polymerase,
1
dNTPs, buffer), 1.2 ml
Primers (blue), 25 µl
1
Allele ladder, 200 µl
1
Orange G loading dye, 1 ml
1
PCR tubes, 0.2 ml
50
Capless PCR tube adaptors, 1.5 ml
50
Colored microcentrifuge tubes, 2.0 ml
60
Curriculum, including teacher’s guide,
1
student manual, and graphic quick guide
Required Accessories Not Included in Kit:
DNA Electrophoresis reagent pack, p. 106
1
Horizontal gel electrophoresis
4–8
chambers, p. 104
Adjustable micropipets, p. 138
2–20 µl
1–8
20–200 µl
1
Pipet tips, aerosol barrier, p. 140
2–20 µl, Xcluda™ B
1 box
20–200 µl, Xcluda D
1 box
Microcentrifuges, p. 134
1–4
Thermal cycler, p. 110
1
Power supplies, p. 130
2–4
Gel staining trays, p. 143
4–8
Lab 1
Recommended (Optional) Accessories:
Adjustable micropipets, p. 138
100–1,000 µl
1
Pipet tips, aerosol barrier, p. 140
100–1,000 µl, Xcluda E
1 box
Rocking platform, pp. 135–136
Microwave oven
Refresh Kit Components: (more info pp. 84, 143)
Crime Scene Investigator PCR
$147.00
Basics kit reagent refill pack
(#166-2601EDU) includes PCR master mix,
primers, allele ladder, orange G loading dye,
crime scene and suspect DNA samples
96-Place PCR tube rack and cover, 5
(#TRC-0501EDU)
Gel staining trays, 4 (#166-0477EDU)
30.40
16.00
2x master mix for PCR, p. 115
DNA electrophoresis reagent packs, p. 106
UView™ 6x loading dye, p. 109
Lab 2
See Bulletin 5396 at explorer.bio-rad.com.
• DNA replication and the
polymerase chain reaction (PCR)
• Chemical properties of biological
molecules
• DNA structure and function
• Chemistry of electrophoresis
Mendelian genetics
Polymorphic loci and multiple alleles
Genetics of noncoding DNA
Short tandem repeats (STRs)
Genetic identity
Structure and function of the human genome
Crime Scene Investigator PCR Basics
Brand Name Genes Curriculum
Download the topical case study senario
format, developed by the University of North
Carolina, Chapel Hill DESTINY Traveling
Science Learning Program. Download free
at explorer.bio-rad.com.
Visit us on the Web at explorer.bio-rad.com
57
PV92 PCR Informatics Kit — AP Big Idea 1: Where Did You Get Those Genes?
PV92 PCR Informatics Kit
Each kit supports 32 students.
PV92 PCR Informatics Kit
Catalog #
List Price . . . . . . . . .EDU Price
166-2100EDU
$262.50 . . . . . . . .$210.00
Ships at room temperature.
Immediately store temperature-sensitive reagents
at –20°C or 4°C as indicated.
The critical first step in preparation for PCR-based DNA profiling is extracting intact genomic
DNA. Our hair follicle and cheek cell DNA extraction procedures produce greater amplification
efficiencies than any other kit available. Your students will get great results. We guarantee it.
Protease Solution
166-2003EDU
43.75 . . . . . . . . . .35.00
This activity will open the door to discussions about technical and ethical aspects of DNA
profiling and genetic screening. Students hunt for a specific Alu repeat (a 300 base pair
repetitive sequence of DNA) on chromosome 16. Over evolutionary time, up to 1 million
copies of the Alu repeat have become randomly inserted throughout the human genome.
Within a specific region on chromosome 16 called PV92, some of us carry an Alu insertion
and some of us do not. Such variations among individuals’ genotypes are inherited —
and are the raw material of genetic diversity and evolution. These subtle variations in
our DNA are evidence of our ancestry and form the basis of personal identification
via DNA fingerprinting.
Required for DNA extraction from hair follicles.
Key Kit Features
• Aligns with AP Biology Big Idea 1; Lab 2
• Extract genomic DNA from students’ cheek
cells or hair follicles
• Amplify student and positive control DNA
• Analyze student allelic frequencies
• Compare classroom genetic composition with
other populations around the world
• Complete in three 45 minute lab sessions
7 x 10 cm ReadyAgarose™ gel runs
in the Mini-Sub® cell GT cell
(see page 104)
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
• Use of PCR and DNA gel
electrophoresis in DNA profiling
• Use of positive experimental controls
• Use of bioinformatics databases
• Apply the Hardy-Weinberg equation
to student data
Ecosystems,
Environment,
and
Interactions
• Eukaryotic cell structure
and organization
• Tissue types for biological
sampling
• Function of genetic diversity
• Genetic variation in the
human genome
• Selective advantages of
heterozygous alleles
58
1-800-4BIORAD (1-800-424-6723)
Science and
Engineering
Practices
PV92 PCR
Informatics
Kit
Cellular
Proccesses
Evolution
Lab Preparation Checklist
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
The polymerase chain reaction (PCR) is a molecular biology technique that enzymatically
replicates DNA, allowing a small amount of the DNA molecule to be amplified many times in
an exponential manner. PCR is commonly used in detecting hereditary diseases, creating
DNA fingerprints, diagnosing infectious diseases, cloning genes, testing paternity, and
computing DNA. It has been said that the process of PCR is like finding a needle in a
haystack and then making a haystack out of a needle.
• Bioinformatics to compare
class data to worldwide
population data
• Molecular genetics to study
human migration patterns
• Role, place, limits, and possibilities
of science and technology
CLASSROOM KITS: PV92 PCR INFORMATICS KIT — AP BIG IDEA 1
Finally, a wet lab to teach the Hardy-Weinberg equation! The polymerase chain
reaction (PCR) is widely used in forensics, diagnostics, and archeological procedures. In
this activity, your students use real-world forensic techniques to extract DNA from their hair
follicles or cheek cells, and then use PCR amplification and electrophoresis to fingerprint
their own DNA at a specific genetic locus. Using their own results, students test HardyWeinberg equilibrium theory within their classroom population, then go online to compare
classroom results to genetic data of populations worldwide.
Positive controls: homozygous (+/+),
1 each
homozygous (–/–), heterozygous (+/–),
100 µl each
PCR master mix (Taq DNA polymerase,
1
dNTPs, buffer), 1.2 ml
PCR primers, 25 µl
1
DNA molecular mass ruler, 100 µl
1
™
1
InstaGene DNA extraction matrix, 20 ml
Orange G loading dye, 1 ml
1
Microcentrifuge tubes, 1.5 ml
60
PCR tubes, 0.2 ml
50
Screwcap microcentrifuge tubes, 1.5 ml
50
Capless PCR tube adaptors, 1.5 ml
50
Agarose powder, 5 g
1
Electrophoresis buffer, 50x TAE, 100 ml
1
™
1
Fast Blast DNA stain, 500x, 100 ml
Curriculum including teacher’s guide,
1
student manual, and graphic quick guide
Required Accessories Not Included in Kit:
Protease solution (for extraction from
1
hair follicles), p. 58
Horizontal gel electrophoresis
4–8
chambers, p. 104
Adjustable micropipets, p. 138
2–20 µl
1–8
20–200 µl and 100–1,000 µl
1 each
Pipet tips, aerosol barrier, p. 140
2–1,000 µl, Xcluda™ B, D, and E
Power supplies, p. 130
2–4
Thermal cycler, p. 110
1
Microcentrifuges, p. 134
1–4
Gel staining trays, p. 143
4–8
Foam floating racks, p.143
8
Lab 1
Recommended (Optional) Accessories:
Water bath or dry bath, pp. 136–137
Rocking platform, pp. 135–136
Gel support film, p. 106
Vortexer, p. 135
Microwave oven
Refresh Kit Components: (more info pp. 84, 143)
PV92 PCR kit TS reagent refill pack $105.00
(#166-2119EDU) includes PCR primers,
positive controls, DNA molecular mass ruler,
PCR master mix containing dNTPs, buffer,
DNA polymerase, orange G loading dye
PV92 PCR kit RT reagent refill pack
76.00
(#166-2139EDU) includes InstaGene matrix,
Fast Blast DNA stain, agarose, 50x TAE
Gel staining trays, 4 (#166-0477EDU)
16.00
Jellyfish foam floating racks, 8 racks
30.00
(#166-0479EDU)
2x master mix for PCR, p. 115
DNA electrophoresis reagent packs, p. 106
InstaGene matrix, p. 116
UView™ 6x loading dye, p. 109
Lab 2
Molecules to
Organisms
Genetics and
Heredity
•
•
•
•
•
• DNA extraction techniques
• DNA replication and PCR
• DNA structure, function, and
chemistry
• Chemical properties of
biological molecules
Mendelian genetics
Homozygous vs. heterozygous alleles
Inheritance of dimorphic loci
Genetics of noncoding DNA
Short repetitive interspersed elements, SINEs
See Bulletin 5396 at explorer.bio-rad.com.
Bioinformatics link
Following electrophoresis of PCR products, students
can enter class results into the Allele Server of
Cold Spring Harbor Laboratory’s Dolan DNA
Learning Center. Test Hardy-Weinberg equilibrium
theory within your classroom populations, then
compare your classroom to the genetic
composition of populations around the world.
Lab 3
Visit us on the Web at explorer.bio-rad.com
59
New Fish DNA Barcoding Kit — AP Big Ideas 1, 2, 3, and 4: What Kind Is Your Fish?
New Fish DNA Barcoding Kit
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation).
Each kit supports 32 students.
FIsh DNA Barcoding Kit
Catalog #
List Price . . . . . . . . .EDU Price
166-5100EDU
$343.75 . . . . . . . .$275.00
Includes reagents for DNA extraction and PCR for up to
16 fish samples. Sequencing module sold separately.
Ships at room temperature. Immediately store temperaturesensitive reagents at –20°C or 4°C as indicated.
DNA Barcoding Sequencing Module
166-5115EDU
123.75 . . . . . . . . . .99.00
DNA barcoding is also being actively used by the U.S. Food and Drug Administration
(U.S. FDA) to monitor the labeling of fish in the marketplace. Once a fish has been
processed for sale, it can be incredibly difficult for a consumer to tell the difference
between the varieties available. Mislabeling might be done on purpose, for profit, or
accidentally, but the end result to consumers is the same — they do not get what
they pay for.
Includes prepaid sequencing service for up to 9 samples.
Includes shipping of samples within the U.S. (50 states).
Valid for use only with 166-5100EDU. U.S. only.
Key Kit Features
• Aligns with AP Biology Big Ideas 1, 2, 3 and 4;
Lab 3
• Extract and amplify DNA from up to 16 fish
samples
• Amplify sample and positive control DNA
using PCR
• Perform DNA electrophoresis
• Sequence DNA samples and carry out
bioinformatics analysis, including BLAST, to
identify the species
• Inquiry based
Investigate fish fraud! Your students will extract DNA from fish tissue, amplify the
DNA through PCR, perform agarose gel electrophoresis to verify PCR products,
sequence the COI gene, and then use bioinformatics to genetically identify the species
using the sequence obtained. The unique COI sequence produced for a given fish
sample is considered to be its DNA barcode. This exercise is an opportunity for
students to perform an investigation in their local area to see if marketplace
substitution is occurring, or to develop the skills to participate in the iBOL initiative
and contribute scientific data to the Barcode of Life Database.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
•
•
•
•
Use of PCR and DNA gel electrophoresis in DNA profiling
Use of positive experimental controls
Use of bioinformatics databases
Apply the Hardy-Weinberg equation to student data
Ecosystems,
Environment,
and
Interactions
• Bioinformatics to compare
class data to worldwide
population data
• Molecular genetics to study
human migration patterns
• Role, place, limits, and possibilities
of science and technology
• Eukaryotic cell structure
and organization
• Tissue types for biological
sampling
• Function of genetic diversity
• Genetic variation in the
human genome
• Selective advantages of
heterozygous alleles
60
1-800-4BIORAD (1-800-424-6723)
Science and
Engineering
Practices
Fish DNA
Barcoding
Kit
Cellular
Proccesses
Evolution
Lab Preparation Checklist
Molecules to
Organisms
Genetics and
Heredity
•
•
•
•
•
• DNA extraction techniques
• DNA replication and PCR
• DNA structure, function, and
chemistry
• Chemical properties of
biological molecules
Resuspension solution, 5 ml
1
Lysis solution, 5 ml
1
Neutralization solution, 5 ml
1
Matrix, 5 ml
1
Wash buffer, 10 ml
1
Spin columns
20
Master mix for PCR, 1.2 ml
1
Fish primer mix, 50 µl
1
UView™ 6x loading dye, 0.2 ml
1
pCOI control for PCR, 25 µl
1
Sterile water, 2.5 ml
1
PCR molecular weight ruler, 0.2 ml
1
PCR tubes, 0.2 ml
50
Microcentrifuge tubes, 2 ml
250
Curriculum including teacher’s guide,
1
student manual, and graphic quick guide
Required Accessories Not Included in Kit:
Adjustable micropipets, p. 138
2–20 µl, 20–200 µl and 100–1,000 µl 8 each
Pipet tips, p. 140
2–20 µl aerosol barrier
1 box
20–200 µl aerosol barrier
1 box
100–1,000 µl
1 box
50x TAE, 100 ml (166-0742EDU)
1
Water bath or dry bath, pp. 136-137
1
Thermal cycler, p. 110
1
Molecular biology grade agarose, 5 g, p. 106
1
Power supplies, p. 130
2–4
Horizontal electrophoresis chambers, p. 104 2–8
Microcentrifuge tube racks, p. 142
8 racks
PCR tube racks, p. 142
8 racks
UV transilluminator or imaging system,
365 nm, p. 132
1
Microcentrifuge with variable speed
1–4
setting capable of 14,000 x g, p. 134
Marking pens
8
Razor blades or plastic knives (new)
16
Weigh boats
16
Ethanol, 95%
10 ml
Graduated cylinders; 100 ml, 500 ml, 3 L 1 ea
Microwave oven or magnetic hot plate
1
with stir bar
Bottle or Erlenmeyer flask, 1 L
1
Distilled water
Gloves, latex or nitrile
Beakers for ice bath
1
Obtain fish samples locally
1–16
Parafilm
1 roll
Zipper sealed bag for mailing sequencing samples
See Bulletin 5396 at
explorer.bio-rad.com.
CLASSROOM KITS: FISH DNA BARCODING KIT — AP BIG IDEAS 1, 2, 3, AND 4
The International Barcode of Life (iBOL) project is a global initiative to
identify all species on Earth using a technology called DNA barcoding.
DNA barcoding adds a level of genetic identification to species classification.
Traditionally, a taxonomist would make species determinations based on observations
of physical and environmental characteristics of a specimen. In some cases, DNA
barcoding can help make the distinction between species that look and behave so
similarly that they would be considered the same species based on the traditional
taxonomical criteria. Since the slight nuances found through DNA
sequencing reveal the uniqueness of a species, these
sequences are essentially barcodes that can be used
to help identify previously unclassified species. The
cytochrome c oxidase 1 (COI) gene is a mitochondrial
gene found in fish, birds, butterflies, flies, and other
animal groups, making it a useful benchmarking tool
for comparison between species.
Recommended (Optional) Accessories:
ReadyAgarose™ precast mini gels, p. 107
Gel staining trays, p. 143
Refresh Kit Components: (more info pp. 84, 143)
DNA Extraction Refill pack, includes
$95.00
resuspension, lysis, and neutralization
solutions, wash buffer, matrix, and
20 spin columns (166-5105EDU)
Fish DNA Barcoding Temperature170.00
Sensitive Refill pack, contains 2x
master mix for PCR, fish primer mix,
pCOI positive control DNA, UView
6x loading dye, sterile water (166-5110EDU)
Lab 1
0.2 ml PCR tubes (TWI-0201EDU), p. 111
2 ml microtubes (223-9430EDU), p. 143
2x master mix for PCR, p. 115
UView 6x loading dye, p. 109
Mendelian genetics
Homozygous vs. heterozygous alleles
Inheritance of dimorphic loci
Genetics of noncoding DNA
Short repetitive interspersed elements, SINEs
Visit us on the Web at explorer.bio-rad.com
61
GMO Investigator™ Kit: Have Your Favorite Foods Been Genetically Modified?
GMO Investigator Kit
Lab Preparation Checklist
Kit (166-2500EDU) contains sufficient
materials for 8 student workstations
(2–4 students per workstation).
Students engage in a complete investigation in which they gather sample food items from
the grocery store, extract DNA from the samples, amplify the DNA using PCR, and use gel
electrophoresis to identify the presence or absence of amplified GMO sequences.
Each kit supports 32 students.
GMO Investigator Kit
The GMO Investigator kit uses PCR and DNA electrophoresis to test for the presence of two
different GMO-associated DNA sequences: the 35S promoter of the cauliflower mosaic virus,
and the terminator of the nopaline synthase gene of Agrobacterium tumefaciens. These DNA
sequences are present in most (>85%) of the GM crops that are approved for distribution worldwide. In addition, the integrity of the plant DNA extracted from food is tested by using PCR to
amplify a section of the photosystem II chloroplast gene that is common to most higher plants.
Catalog #
List Price . . . . . . . . .EDU Price
166-2500EDU
$231.25 . . . . . . . .$185.00
Ships at room temperature. Immediately store reagents
at –20°C or 4°C as indicated. Electrophoresis reagents
not included — available separately.
Small Fast Blast™ DNA Electrophoresis
Reagent Pack
166-0450EDU
105.00 . . . . . . . . . .55.00
The kit allows a guided inquiry approach to this exercise. Students conduct sophisticated
scientific procedures employing multiple levels of controls that allow them to assess the
validity of their results. They determine the presence or absence of GMO sequences in their
test food and answer the questions: did we successfully extract DNA; did our PCR work as
expected, and do we have contamination?
To pour, run, and stain forty-eight 1% or sixteen 3%
7 x 10 cm agarose gels
GMO Investigator Kit Plus Small Fast Blast
DNA Electrophoresis Reagent Pack
166-2550EDU
287.50 . . . . . . . . .230.00
Are GM crops a good thing? Many people who object to the use of GM crops argue
that there is a potential for “superweeds” to arise through cross-pollination with herbicideresistant crops. They also argue that “superbugs” will not be sensitive to the toxins in
pest-resistant crops. Many are concerned about potential allergic reactions to novel proteins,
antibiotic resistance arising from the selectable markers used to develop the crops, or other
unforeseen effects on public health. Some voice concerns that we have not done enough
research to fully understand the implications of altering the planet’s plant diversity.
Key Kit Features
• Extract and amplify DNA from eight food
samples
• Perform genuine diagnostic procedures
• Use PCR and electrophoresis
• Complete in three 45 minute lab sessions
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Proponents of GM crops and foods argue that these crops are beneficial for the environment
because they reduce the use of herbicides and pesticides that are toxic to the environment
and human health. In addition, GM crops may preserve arable land by reducing stresses on
the land, improve the nutritional value of food for developing countries, and allow crops to be
grown on previously nonarable land.
Regardless of where your students stand on the GM debate, won’t they be interested to
know how much of the corn- or soy-based foods they eat have been genetically modified?
• Use of PCR to detect genetically
modified organisms
• DNA gel electrophoresis
• Use of positive and negative
experimental controls
• Interpretation of experimental results
7 x 10 cm ReadyAgarose™ gel runs
in the Mini-Sub® cell GT cell
(see p. 104)
Ecosystems,
Environment,
and
Interactions
• Pesticides and herbicides
• Population growth and
environmental challenges
• Allergies and immune response
• Agricultural science
• Role, place, limits, and possibilities
of science and technology
• Plant physiology and cell structure
• Plant cell transformation and
totipotency
• Chloroplast structure and function
•
•
•
•
Implications of genetic manipulation
Plant biodiversity and ecosystems
Co-evolution of pests and plants
Population growth and
environmental challenges
• Gene conservation
62
1-800-4BIORAD (1-800-424-6723)
Science and
Engineering
Practices
GMO
Investigator
Cellular
Proccesses
Evolution
CLASSROOM KITS: GMO INVESTIGATOR KIT
Genetically modified (GM) foods do not require labeling as such in the U.S., and foods
with less than 5% content from genetically modified organisms (GMOs) can be labeled
“GMO free.” In much of Europe and Asia, GM foods require labeling even if they contain
>1% GM content. This kit is designed to allow students to test their favorite foods for the
presence of GM content.
Kit
•
•
•
•
•
•
1
1
1
1
1
1
1
1
20
60
50
50
50
1
Required Accessories Not Included in Kit:
DNA Electrophoresis Reagent Pack, p. 106 1
Obtain food samples locally
Horizontal gel electrophoresis
4–8
chambers, p. 104
Adjustable micropipets, p. 138
2–20 µl
1–8
20–200 µl
1
Pipet tips, aerosol barrier, p. 140
™
2–20 µl, Xcluda B
1 box
20–200 µl, Xcluda D
1 box
Water bath or dry bath, pp. 136–137
1
Microcentrifuges, p. 134
1–4
Thermal cycler, p. 110
1
Power supplies, p. 130
2–4
Mortars and pestles
1–8
Balance with range 0.5–2 g
1
Weigh boats or paper
16
Gel staining trays, p. 143
4–8
Foam floating racks, p.143
8
Recommended (Optional) Accessories:
Rocking platform, pp. 135–136
Microwave oven
• DNA extraction techniques
• DNA replication and PCR
• DNA structure, function,
and chemistry
• Chemical properties of
biological molecules
• Plant photosynthesis genes
Genetic engineering to create GMOs
Transcription factors
Gene regulation and expression
Traditional vs. GM crop breeding
Mendelian genetics
Expression and regulation of genes
in foreign hosts
Lab 1
Lab 2
Refresh Kit Components: (more info pp. 84, 143)
GMO Investigator kit reagent
$128.00
refill pack (#166-2501EDU) includes
PCR master mix, GMO primers, plant
PSII primers, GMO positive control DNA,
non-GMO food control, PCR molecular
weight ruler, orange G loading dye
Disposable plastic transfer pipets,
sterile, 500 (#166-0474EDU)
Jellyfish foam floating racks, 8 racks
(#166-0479EDU)
Gel staining trays, 4 (#166-0477EDU)
105.00
30.00
16.00
InstaGene matrix, p. 116
2x master mix for PCR, p. 115
DNA electrophoresis reagent packs, p. 106
UView™ 6x loading dye, p. 109
Molecules to
Organisms
Genetics and
Heredity
Bio-Rad certified non-GMO food control
InstaGene™ matrix, 20 ml
GMO positive control DNA, 500 µl
PCR master mix (Taq DNA polymerase,
dNTPs, buffers), 1.2 ml
GMO primers (red), 15 µl
Plant PSII primers (green), 15 µl
PCR molecular weight ruler, 200 µl
Orange G loading dye, 1 ml
Disposable plastic transfer pipets
Microcentrifuge tubes, 1.5 ml
Screwcap microcentrifuge tubes, 1.5 ml
PCR tubes, 0.2 ml
Capless PCR tube adaptors, 1.5 ml
Curriculum, including teacher’s guide,
student manual, and graphic quick guide
DNA Model, p. 117
(#166-7015EDU)
Lab 3
24.99
See Bulletin 5396 at explorer.bio-rad.com.
Visit us on the Web at explorer.bio-rad.com
63
Real-Time PCR Kits: For Crime Scene Investigator PCR Basics™ and GMO Investigator™ Kits
How much DNA is there? Using genomic DNA as the template for amplification,
real-time PCR can be used in infectious disease diagnostics to rapidly determine levels of
specific pathogens in various tissues. The molecular diagnostic lab also relies heavily on
real-time PCR for detection of aneuploidies and the diagnosis of other genetic diseases.
In microbiology labs, real-time PCR can be used to detect and quantitate various microbial
contaminants in environmental samples.
Lab Preparation Checklist
Kit contains sufficient materials for 8 student
workstations (2–4 students per workstation), plus
additional real-time reagents for further studies.
Each kit supports 32 students.
Crime Scene Investigator PCR Basics
Real-Time PCR Starter Kit
Catalog #
List Price . . . . . . . . .EDU Price
166-2660EDU
$676.25 . . . . . . . .$541.00
GMO Investigator Real-Time PCR
Starter Kit
166-2560EDU
686.25 . . . . . . . . .549.00
The Bio-Rad Crime Scene Investigator PCR Basics kit is a tool for teaching students the
principles of PCR and its use in forensic DNA analysis. Using the Crime Scene Investigator
PCR Basics kit to teach real-time PCR is a good starting point for novices to become familiar
with real-time PCR techniques. Additionally, DNA fingerprints can still be investigated using
gel electrophoresis and melt curve analysis, showing how real-time and conventional PCR
can be complementary techniques.
The Bio-Rad GMO Investigator kit is a tool for teaching students the principles of PCR
and its use in testing foods for genetic modifications. Using real-time PCR with the GMO
Investigator kit can show how much plant DNA recovered from and compare how much
genetically modified organism (GMO) DNA is in each food sample. It is even possible
to determine what fraction of a food product has been made with genetically modified
ingredients in the same manner standard testing labs do.
*
Real-Time PCR Kits
The above kits have components that ship at room
temperature and at 4°C. Immediately store temperaturesensitive reagents at –20°C or 4°C as indicated.
Key Kit Features
• Quantitate DNA
• Discover key differences between conventional
and real-time PCR analysis
• Analyze and evaluate real-time PCR results
• Perform melt curve analysis
• Determine the accuracy and reliability of
pipetting techniques
• Learn the molecular basis of DNA amplification
reactions using real-time PCR detection systems
Ecosystems,
Environment,
and
Interactions
• Genetic diversity and
individual identification
64
1-800-4BIORAD (1-800-424-6723)
Science and
Engineering
Practices
Real-Time
PCR Kits
Cellular
Proccesses
Evolution
1
iQ™ SYBR® Green supermix, 5 ml
Agarose powder, 25 g
Electrophoresis buffer, 50x TAE, 100 ml
Fast Blast™ DNA stain, 500x, 100 ml
Sterile water, 500 ml
PCR tube strips
Optical flat caps
Curriculum, including teacher’s guide
and student manual
1
1
1
1
1
120
120
1
Required Accessories Not Included in Kits:
Horizontal gel electrophoresis
4–8
chambers, p. 104
Adjustable micropipets, p. 138
2–20 µl
1–8
20–200 µl, 100–1,000 µl
1 each
Pipet tips, aerosol barrier, p. 140
2–1,000 µl, Xcluda™ B, D, and E
Power supplies, p. 130
2–4
Real-time thermal cycler, p. 112
1
Microcentrifuges, p. 134
1–4
Additional Required Accessories Not Included
in GMO Investigator Real-Time PCR Starter Kit:
Obtain food samples locally
Water bath or dry bath, pp. 136–137
1
Mortars and pestles
1–8
Balance with range 0.5–2 g
1
Weigh boats or paper
16
Refresh Kit Components: (more info pp. 84, 143)
Crime Scene Investigator PCR
$147.00
Basics kit reagent refill pack
(#166-2601EDU), see p. 57
• Comparison of conventional and
real-time PCR analysis
• Quantitation of DNA
• Use of experimental controls
• Interpretation of experimental results
• Eukaryotic cell structure
and organization
• Tissue types and biological
sampling
Crime Scene Investigator PCR Basics kit
or GMO Investigator kit
(based on which package chosen)
Recommended (Optional) Accessories:
Rocking platform, pp. 135–136
Microwave oven
Pray, L (2004). Consider the cycler. The Scientist.
www.the-scientist.com/?articles.view/articleNo/15904/title/Consider-the-Cycler/. Accessed January 7, 2014.
• Detection of microbial
contaminants in
environmental samples
• Viral load quantitation
• Genetic screening
• Role, place, limits of
science and technology
CLASSROOM KITS: REAL-TIME PCR KITS
Real-time PCR is the diagnostic technique of the present and future. PCR is
now such a fundamental technique in the biotechnology lab that it has been said “PCR is
to biology what petroleum is to transportation.”* It forms a basis for multiple ways to analyze
and detect nucleic acids ranging from DNA fingerprinting to DNA sequencing to mutagenesis.
Real-time PCR is becoming the most widely used application of PCR in the research lab for
genomic and gene expression analysis and is rapidly establishing itself as a technique in the
clinical diagnostic lab. Real-time PCR is an extremely valuable analytical tool that not only
reveals what DNA is present, but how much. The need for faster, more accurate, and more
economical systems with a high throughput has fueled the popularity of real-time PCR.
Molecules to
Organisms
Genetics and
Heredity
• DNA replication and the
polymerase chain reaction (PCR)
• Chemical properties of biological
molecules
• DNA structure and function
• Chemistry of electrophoresis
• Chemistries for monitoring
real-time PCR
• Mendelian genetics
• Genetic identity
• Structure and function of genomes
GMO Investigator kit reagent refill
pack (#166-2501EDU), see p. 61
128.00
Sterile water, 500 ml (#163-2091EDU)
30.40
Real-Time PCR Application Guide,
16.80
(#170-9799EDU), a detailed review
of real-time PCR theory, technology,
and applications
96-Place PCR tube rack and cover, 5
30.40
(#TRC-0501EDU)
Gel staining trays, 4 (#166-0477EDU)
16.00
InstaGene™ matrix, p. 116
2x master mix for PCR, p. 115
DNA electrophoresis reagent packs, p. 106
iQ™ SYBR® Green supermix, p. 114
PCR strip tubes and optical flat caps, p. 111
DNA Model, p. 117
(#166-7015EDU)
Lab 1
24.99
Real-Time PCR Application Notes
Download our application notes for converting
these kits into real-time PCR applications.
Download free at explorer.bio-rad.com.
Visit us on the Web at explorer.bio-rad.com
65
Classroom Kits: Cloning and Sequencing Explorer Series
Section Contents
Complete Cloning and Sequencing Explorer Series . . . . . . . . . . . . . . . . . . 68
Nucleic Acid Extraction Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Prepare your students for the real world of scientific
research. Engage them with the opportunity to perform
novel, relevant research that can actually contribute to
scientific knowledge.
GAPDH PCR Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Electrophoresis Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
PCR Kleen™ Spin Purification Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
In this unique modular lab series, students are guided
through an innovative research workflow identical to
those performed in genomics labs worldwide. Over a
multiple-week lab course, students will combine traditional
and cutting-edge molecular biology techniques and
bioinformatics. Your students will clone, sequence, and
analyze a housekeeping gene from a plant of your choice,
allowing each class to produce original data.
Ligation and Transformation Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Microbial Culturing Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Aurum Plasmid Mini Purification Module . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Sequencing and Bioinformatics Module . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
The real thing. As a research and diagnostics
manufacturing company, Bio-Rad brings unique strengths
to the education community including credibility, highquality products (kits and equipment that work), novel
inquiry-based curricula, cutting-edge kit applications,
competitive pricing, superior teacher professional
development programs, long-term strategic partners
and allies, and strong personal endorsements from
customers across all levels of education.
GAPDH PCR
Module
• Nested PCR
• Degenerate primers
• Exonuclease treatment
Nucleic Acid
Extraction
Module
• Genomic DNA extraction
• Cellular membrane
disruption
• Isolation of genomic DNA
66
1-800-4BIORAD (1-800-424-6723)
Microbial
Culturing
Module
Plasmid
Purification
Module
• Grow transformed cells
• Cell selection
• Ampicillin resistance
• Plasmid isolation
• Restriction enzyme
digestion
PCR
Purification
Module
Ligation and
Transformation
Module
DNA
Sequencing
Module
• Size exclusion
chromatography
• Blunting of PCR products
• Ligation
• Generation of competent
bacteria
• Bacterial transformation
• Sterile technique
• Directional sequencing
primers
• Automated sequencing
Electrophoresis
Module
Bioinformatics
Module
• Agarose gel analysis
• PCR product visualization
•
•
•
•
•
Data analysis
Contig assembly
Intron/exon prediction
Chromatogram analysis
BLAST searches
“
Bio-Rad has made a huge
difference in simplifying
difficult concepts so we
can have more confidence
that we will obtain positive
results in the lab.
CLASSROOM KITS: CLONING AND SEQUENCING EXPLORER SERIES
Cloning and Sequencing Explorer Series . . . . . . . . . . . . . . . . . . . . . . . 66
”
Clint Coleman
Delgado Community College
New Orleans, Louisiana
67
Cloning and Sequencing Explorer Series: Cloning Pieces of the Puzzle
From DNA extraction to computer-based sequence analysis, this modular kit is designed
as a six- to eight-week series of lab activities in which students clone and analyze a
plant housekeeping gene: glyceraldehyde 3-phosphate dehydrogenase (GAPDH).
Cloning and Sequencing Explorer Series
The Complete Cloning and Sequencing series
supports 12 workstations.
See pages 70–75 for complete module descriptions
or to order modules separately.
Cloning and Sequencing Explorer
Series — All Eight Modules
Catalog #
List Price . . . . . . . .EDU Price
166-5000EDU $1,906.25 . . . . .$1,525.00
This lab course provides your students with the opportunity to perform novel research,
allowing them to clone and sequence a gene from an uncharacterized plant species
and to add to the body of scientific knowledge around the world. The series provides a
fully developed and ready-to-go lab course including relevant background, protocols
that work, and student assessment.
Students will extract genomic DNA from their chosen plant sources, use
degenerate primers to perform nested PCR, and amplify a major portion of the GAPC
gene — a housekeeping gene in the GAPDH family that is essential for the most basic
of biological processes: respiration.
Students will then clone the gene fragment, transform it into bacteria, screen
their clones using plasmid minipreps and restriction enzymes, and send positive clones
to be sequenced (sequencing service not included).
On receiving their novel sequences, students will perform extensive bioinformatics
analysis on their sequences, including BLAST searches, assembling forward and
reverse sequences into contigs, identifying introns and exons, and transcribing gene
sequences into protein with user-friendly, bioinformatics tools.
Upon completion of the lab course, students may elect to submit novel sequences to
the National Institutes of Health National Center for Biotechnology Information (NCBI)
databases, thereby making their research available to the worldwide scientific community.
Ships with both temperature sensitive and room
temperature components. Immediately store temperature
sensitive items at 4°C or –20°C as indicated.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Workflow
The lab is presented over the next few
pages in a series representing the
experimental workflow:
1. Extract genomic DNA from plants.
2. Amplify the GAPDH gene using
PCR and analyze.
3. Purify the PCR product.
4. Ligate the PCR product into a
plasmid.
5. Transform the ligated plasmid into
bacteria.
CLASSROOM KITS: CLONING AND SEQUENCING EXPLORER SERIES
Equip your students with technical skills for entry-level lab positions
or inspire them to pursue graduate degrees in scientific research. The Cloning and
Sequencing Explorer series has been designed with the assistance of undergraduate and
community college educators to meet the needs of biotechnology and biology course
instructors who want their students to understand how molecular biology skills and techniques are applied to real-world research projects.
Lab Preparation Checklist
Lab contains sufficient materials for 12 student
workstations (2–4 students per workstation).
Cloning and Sequencing Explorer Series
Contents
Nucleic acid extraction module
1
GAPDH PCR module
1
Small DNA electrophoresis reagents module 1
™
PCR Kleen spin purification module
1
Ligation and transformation module
1
Microbial culturing module
1
Aurum™ plasmid mini purification module
1
Sequencing and bioinformatics module
1
1.5 ml EZ Micro™ test tubes, 500
1
Curriculum resource CD
1
Required Accessories Not Included in Kit:
Plant samples
95 –100% lab grade ethanol
Horizontal gel electrophoresis chambers, p. 104
Thermal cycler, p. 110
Adjustable micropipets, p. 138
Pipet tips, aerosol barrier, p. 140
Incubation oven, p. 137
Water bath and dry bath, pp. 136–137
Microcentrifuges capable of greater than
12,000 x g, p. 134
Power supplies, p. 130
Gel documentation system, p. 132
Shaking water bath or shaking incubator
Recommended (Optional) Accessories:
Vortexer, p. 135
Microwave oven/autoclave
Spectrophotometer and cuvettes, p. 131
Aurum vacuum manifold, p. 116
Available Separately:
Nucleic acid extraction module
(#166-5005EDU)
$169.00
GAPDH PCR module (#166-5010EDU) 399.00
This laboratory course is designed to ensure success for you and your students.
Tailored controls permit the continuation of the experiment to its conclusion. This allows
students to make mistakes, learn from them, and still be exposed to every step of the
workflow within a specific time period.
6. Grow minipreps of the transformed
bacteria.
Small UView™ DNA electrophoresis
reagents module (#166-0462EDU)
87.00
7. Isolate plasmid DNA and analyze
by restriction digestion.
PCR Kleen spin purification module
(#732-6300EDU)
63.00
Students will experience firsthand the satisfaction that comes from a completed and
successful research project, which may encourage them to pursue a career in research.
8. Sequence plasmid DNA.
(Sequencing service not included)
Ligation and transformation module
(#166-5015EDU)
320.00
9. Analyze GAPDH gene sequence
using bioinformatics.
Each module within the series is also
available as a stand-alone kit, providing
the opportunity to use individual kits to
update existing curricula or to design
and develop your own unique series of
experiments.
Microbial culturing module
(#166-5020EDU)
96.00
Aurum plasmid mini purification
module (#732-6400EDU)
152.00
Sequencing and bioinformatics
module (#166-5025EDU)
262.00
1.5 ml EZ Micro™ test tubes, 500
(#223-9480EDU)
23.20
Curriculum resource CD
(#166-5001EDU)
29.00
Refresh Kit Components:
Please visit explorer.bio-rad.com and request
bulletins 5872 (purchasing guide, printable),
5905 (interactive purchasing guide), and 5891
(large class/multiple class preparation guide)
for more information.
68
1-800-4BIORAD (1-800-424-6723)
Visit us on the Web at explorer.bio-rad.com
69
Cloning and Sequencing Explorer Series: Cloning Pieces of the Puzzle
To clone a gene, DNA must first be
extracted from the organism. After choosing
two plants to study, students collect plant
tissue, grind the tissue in lysis buffer using
micropestles, centrifuge the lysate to remove
cellular debris, mix the lysate with ethanol, and
apply to a silica spin column. Spin columns are
washed three times and all nucleic acids are
eluted from the column. The eluate can be
used directly for PCR.
Different plants yield different quantities of
DNA. Each extraction yields sufficient DNA for
16 PCR reactions. This protocol has been
optimized for plant DNA extraction with
reagents to reduce polyphenol inhibition of
PCR — a list of plants demonstrated to yield
amplifiable DNA with the GAPDH primers is
provided, along with a list of plants that are
less successful with this protocol — due to
either issues with DNA extraction or lack of
homology to GAPDH primers.
Alternatively, have your students experience
the risks inherent to real-world research and
use untested plants, or hedge your bets and
use one recommended plant and one novel
plant. The Cloning and Sequencing Explorer
Series GAPDH PCR module contains purified
genomic DNA from Arabidopsis as a control,
which allows continuation of the experiment
in the unlikely event that students’ extracted
DNA does not amplify!
On your own:
Use DNA extracted with this
module for independent research
projects and existing lab PCR protocols.
Quantitate DNA concentration using fluorometry
or examine the total nucleic acid content from
cells by gel electrophoresis. Genomic DNA
appears as a faint band at the top of the gel,
while RNA is visible as two major bands much
further down the gel. RNA can be removed
from samples by treatment with RNase I, or
similarly, DNA can be removed with DNase I
treatment. For further RNA work, elution
using DEPC-treated water is
recommended.
GAPDH PCR: As part of the
Cloning and Sequencing Explorer series
Nucleic Acid Extraction Module
Kit supports 12 student workstations.
Nucleic Acid Extraction Module
Catalog #
List Price . . . . . . . . .EDU Price
166-5005EDU
$211.25 . . . . . . . .$169.00
Ships at room temperature. Immediately store DTT at 4°C.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Lab Preparation Checklist
Kit contains sufficient materials for 12 student
workstations or 25 genomic DNA extractions.
Lysis buffer, 20 ml
Dithiothreitol (DTT), 0.3 g
Wash buffer, 20 ml
Sterile water, 2.5 ml
Micropestles
Spin columns
Microcentrifuge tubes, 2.0 ml
Microcentrifuge tubes, 1.5 ml
Colored microcentrifuge tubes, 2.0 ml
Instruction manual
1
1
1
1
25
25
25
30
60
1
How do you amplify a gene when you do not
know its sequence? The experimental goal of this
module is to amplify a conserved gene from a novel
genome. Glyceraldehyde 3-phosphate dehydrogenases
(GAPDHs) are a family of enzymes essential for
glycolysis — one of the most fundamental metabolic
processes of life. They are found in all organisms
and, because their enzyme function is so vital to life,
their protein sequences are highly conserved both
within the family and between organisms. Thus, to
amplify a highly conserved gene from a novel plant
should be simple; just design primers to the
conserved regions and amplify — right?
As ever, biology is not so simple — protein
sequence is not the same as gene sequence. What
about the problems of noncoding introns and the
degeneracy of the genetic code? Your students
will discover how using degenerate primer
sequences and multiple (nested) rounds of PCR
can help solve these problems. Ultimately, students
will learn how PCR is adapted in real-world research.
In this lab, students will amplify genomic DNA,
via two rounds of PCR, from Arabidopsis (provided
as a control) and purified genomic DNA from any two
novel plants. The novel plant DNA is extracted using
the nucleic acid extraction module.
Required Accessories Not Included in Kit:
Plant samples
95–100% lab grade ethanol, 300 ml
1
Adjustable micropipets, p. 138
2–20 µl
1
20–200 µl
1–12
100–1,000 µl
1–12
Pipet tips, aerosol barrier, p. 140
™
1 box
2–20 µl, Xcluda B
20–200 µl, Xcluda D
1–12 boxes
100–1,000 µl, Xcluda E
1–12 boxes
Microcentrifuges capable of greater
1–4
than 12,000 x g, p. 134
Water bath, p. 137
1
Dry bath, p. 136
1
The initial round of PCR uses degenerate primers
to amplify a pool of GAPDH genes. These initial
PCR reactions are then treated with the enzyme
exonuclease I, which digests any remaining
degenerate primers. A second round of nested
PCR is then performed on this pool of templates,
using primers inside the original target sequence
and designed to specifically select a subfamily of
GAPDH genes, GAPC genes.
Recommended (Optional) Accessories:
Vortexer, p. 135
Spectrophotometer and cuvettes, p. 131
DEPC-treated water, 100 ml
(#700-7253EDU)
Students will observe the benefits of nested PCR
compared to a single round of PCR and also learn
that there can be great variability in the same gene
between different plants. The portion of the GAPC
gene amplified varies in size from 0.6 kb to more
than 2 kb, primarily due to differences in intronic
DNA rather than large differences in the coding
sequence between different plants.
Refresh Kit Components: (more info pp. 84, 143)
Nucleic acid extraction reagent refill
pack (#166-5006EDU), includes DTT,
lysis buffer, wash buffer, sterile water
Aurum™ mini columns, 50
(#732-6826EDU)
Students then analyze their PCR products using
agarose gel electrophoresis and visualize the GAPC
gene they have isolated from their plant genome.
GAPDH PCR Module
Kit supports 12 student workstations.
GAPDH PCR Module
Catalog #
List Price . . . . . . . . .EDU Price
166-5010EDU
$498.75 . . . . . . . .$399.00
Ships on blue ice. Immediately store reagents bag at –20°C.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Lab Preparation Checklist
Kit contains sufficient materials for 12 student
workstations.
Initial GAPDH PCR primers, blue, 50 µl
1
Nested GAPDH PCR primers, yellow, 50 µl 1
PCR master mix, 1.2 ml
3
Plasmid control DNA, 1 ml
1
Control Arabidopsis genomic DNA, 20 µl
1
Exonuclease I, 50 µl
1
500 bp Molecular weight ruler, 400 µl
1
1
UView™ 6x loading dye, 1 ml
Sterile water, 2.5 ml
3
PCR tubes, 0.2 ml
150
Capless PCR tube adaptors, 1.5 ml
150
Colored microcentrifuge tubes, 2.0 ml
120
Curriculum, including teacher’s guide,
1
student manual, and graphic quick guide
Required Accessories Not Included in Kit:
Adjustable micropipets, p. 138
2–20 µl
1–12
20–200 µl
1
Pipet tips, aerosol barrier, p. 140
1–12 boxes
2–20 µl, Xcluda™ B
20–200 µl, Xcluda D
1 box
Thermal cycler, p. 110
1
Foam floating racks, p. 143
8
Recommended (Optional) Accessories:
Novel plant genomic DNA
Microcentrifuges, p. 134
Vortexer, p. 135
Refresh Kit Components: (more info pp. 84, 143)
GAPDH PCR reagent refill pack
(#166-5011EDU), includes initial
GAPDH PCR primers, nested GAPDH PCR
primers, PCR master mix, plasmid control
DNA, control Arabidopsis genomic DNA,
exonuclease I, molecular weight ruler,
UView 6x loading dye, sterile water
2x master mix for PCR, p. 115
UView 6x loading dye, p. 109
Electrophoresis: As part of the
Cloning and Sequencing Explorer series
Pour superior agarose gels with our
convenient DNA agarose gel electrophoresis
reagent packs. Whether safety or sensitivity
is your concern, there is an electrophoresis
pack to meet your needs. Electrophoresis
packs are provided in three sizes depending
on your teaching needs: small (16–48 gels),
medium (90–270 gels), and large (360–1,080
gels). Each pack contains the highest quality
molecular biology certified agarose powder,
convenient premixed 50x Tris/acetate/EDTA
(TAE) buffer, and a DNA stain suitable for
your students. For the safety conscious, the
nontoxic Fast Blast™ DNA stain electrophoresis
packs are recommended. For advanced
student activities requiring increased
sensitivity and using UV transillumination,
the UView electrophoresis pack is
recommended, or we have the classic
ethidium bromide electrophoresis packs.
Whichever electrophoresis pack you choose,
your students are guaranteed to produce
great results.
Electrophoresis Module
Kits support 24–96 students. See p. 106 for details.
Electrophoresis Module
Catalog #
List Price . . . . . . . . .EDU Price
166-0450EDU $105.00 . . . . . . . . .$55.00
166-0455EDU
270.00 . . . . . . . . .189.00
166-0460EDU
700.00 . . . . . . . . .560.00
166-0451EDU
130.00 . . . . . . . . . .69.00
166-0456EDU
260.00 . . . . . . . . .182.00
166-0461EDU
667.50 . . . . . . . . .534.00
166-0462EDU* 145.00 . . . . . . . . . .75.00
Ships and stores at room temperature.
Please refer to p. 106 for a complete description of
all the electrophoresis reagent packs.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Lab Preparation Checklist
On your own:
Run your agarose gels in
20 minutes and extend your TAE running
buffer using Bio-Rad’s fast gel protocol.
Request bulletin 5396 from Bio-Rad today.
* Series contains 166-0462EDU, UView DNA
electrophoresis reagent pack. Please note, for the
complete series, the UView 6x loading dye is
included in the GAPDH PCR module.
Agarose powder, 25 g
Electrophoresis buffer, 50x TAE, 100 ml
UView 6x loading dye, 1 ml
1
2
1
CLASSROOM KITS: CLONING AND SEQUENCING EXPLORER SERIES
Nucleic Acid Extraction: As part of the
Cloning and Sequencing Explorer series
Required Accessories Not Included in Kit:
Horizontal gel electrophoresis
4–12
chambers, p. 104
Adjustable micropipets, p. 138
1–12
Pipet tips, p. 140
1–12 boxes
Power supplies, p. 130
3–6
Microwave oven
1
Recommended (Optional) Accessories:
Gel documentation equipment, p. 132
Refresh Kit Components: (more info pp. 84, 143)
Agarose powders, p. 106
Precast agarose gels, p. 107
DNA electrophoresis buffers, p. 107
DNA stains, p. 109
See Bulletin 5396 at explorer.bio-rad.com.
On your own:
This activity provides strong
results and can be performed as a
shorter stand-alone PCR lab to demonstrate
applications of PCR in real research without
going on to clone the gene. Use in conjunction
with an electrophoresis module to visualize
PCR results.
70
Visit explorer.bio-rad.com to view our
comprehensive curriculum manual and see
just how much you can do with this activity!
Kit now contains UView 6x loading dye —
the new safe nontoxic stain!
Visit us on the Web at explorer.bio-rad.com
71
Cloning and Sequencing Explorer Series: Cloning Pieces of the Puzzle
Demonstrate a real-world use of size
exclusion chromatography. Ligation of
PCR fragments is much more efficient
when unincorporated primers, nucleotides,
salts, and enzymes are removed from the
PCR reaction. In this step, PCR products
are purified using simple spin columns that
remove small molecules like salts, enzymes,
and primers by absorbing them into porous
beads. Larger molecules, such as PCR
products greater than 100 bp, cannot enter
the beads and are eluted from the column.
Students first prepare the spin columns by
eluting the storage buffer into a collection tube.
PCR reactions are then applied to the columns,
which are spun at 735 x g for 1 minute.
The purified PCR reaction is collected in a
microcentrifuge tube.
Following this step, both the unpurified and
the purified PCR products can be viewed
side by side using agarose gel electrophoresis
to visually demonstrate the removal of
oligonucleotide primers.
When performing the Cloning and
Sequencing Explorer series, students will
choose the GAPDH gene from one of their
plants to continue their experiments, taking
that gene through cloning and sequencing
and using this module to purify their PCR
product prior to ligation.
On your own:
This module can also be
used in other experiments for cleaning
up any PCR reaction prior to ligation,
removing primers prior to subsequent
nested PCR reactions, or removing
nucleotides before direct DNA sequencing.
PCR Kleen Spin Purification Module
Kit supports 24 student workstations.
PCR Kleen Spin Purification Module
Catalog #
732-6300EDU
Ligation and Transformation: As part of
the Cloning and Sequencing Explorer series
Ligate the PCR product into a plasmid and
transform bacteria. Students directly clone their
PCR products into the pJET1.2 vector, and
immediately transform bacteria using a protocol
that takes less than 2 hours to go from purified
PCR product to transformed bacteria plated
on agar.
Ships and stores at room temperature.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Lab Preparation Checklist
Kit contains sufficient materials for 24 student
workstations or 25 PCR purifications.
PCR Kleen spin columns
Microcentrifuge tubes, 2.0 ml
Microcentrifuge tubes, 1.5 ml
Instruction manual
25
25
25
1
Required Accessories Not Included in Kit:
Adjustable micropipets, p. 138
20–200 µl
1–12
Pipet tips, p. 140
20–200 µl
1–12 boxes
Microcentrifuges capable of greater
1–2
than 12,000 x g, p. 134
Recommended (Optional) Accessories:
Horizontal gel electrophoresis
4–12
chambers, p. 104
Power supplies, p. 130
3–6
Students first remove the 3l-dA overhang from their
PCR product using a proofreading polymerase.
The 3l-dA overhang results from the terminal
transferase activity of Taq polymerase. A 10 minute
protocol is then used to ligate the blunted PCR
product into a pre-opened and blunted vector —
pJET1.2. Successful insertion of a DNA fragment
in pJET1.2 leads to the disruption of a lethal gene
that would otherwise prevent bacterial growth,
allowing for positive selection of recombinant
plasmids. Bacteria that are transformed with
religated vector activate the gene and are killed.
This results in higher transformation efficiency
than traditional blue-white cloning.
BglII restriction
sites are located
on either side
of the pJET1.2
cloning site, which
allow students
to determine
whether their
gene of interest
was successfully
ligated once they
have isolated candidate plasmids.
To transform the ligated plasmids into bacteria,
students first inoculate specialized growth media
and perform a series of microcentrifugations and
washes in a specialized transformation buffer to
make competent cells. These competent cells are
then added to the ligation reactions on ice and
plated directly on warm agar plates.
On your own:
This simple and short
protocol can be used to clone any
PCR product. It takes only about 2 hours
and does not require commercial competent
cells, a refrigerated microcentrifuge, or a
–80°C freezer, making it the method of
choice for educators worldwide!
Microbial Culturing: As part of the
Cloning and Sequencing Explorer series
Microbial Culturing Module
Select for positive ligations and grow bacterial
minipreps. Students use this module to
prepare media for their microbiological needs
for the Cloning and Sequencing Explorer Series.
Kit supports 12 student workstations.
Ligation and Transformation Module
List Price . . . . . . . . .EDU Price
$78.75 . . . . . . . . .$63.00
Ligation and Transformation Module
Catalog #
List Price . . . . . . . . .EDU Price
166-5015EDU
$400.00 . . . . . . . .$320.00
Ships on blue ice. Immediately store reagents bags
at –20°C.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Lab Preparation Checklist
Kit contains sufficient materials for 12 student
workstations.
T4 DNA ligase, 10 µl
2x ligase buffer, 100 µl
Proofreading DNA polymerase, 10 µl
Cloning vector, 10 µl
BglII restriction enzyme, 55 µl
10x restriction enzyme buffer, 100 µl
C- growth medium, 30 ml
Transformation A solution, 5 ml
Transformation B solution, 5 ml
Isopropyl β-D-1-thiogalactopyranoside
(IPTG), 0.1 ml
Sterile water, 1 ml
Colored microcentrifuge tubes, 2.0 ml
Microcentrifuge tubes, 1.5 ml
Instruction manual
1
1
1
1
1
1
1
1
1
1
1
120
30
1
Required Accessories Not Included in Kit:
Adjustable micropipets, p. 138
0.5–10 µl
1–12
Pipet tips, p. 140
0.5–10 µl
1–12 boxes
Water bath, p. 137
1
Microcentrifuge, p. 134
1
Incubation oven, p. 137
1
Shaking water bath or shaking incubator
1
Refresh Kit Components: (more info pp. 84, 143)
Ligation module reagent refill pack
(#166-5016EDU), T4 DNA ligase,
ligase buffer, proofreading DNA
polymerase, cloning vector,
sterile water
Transformation module reagent refill
pack (#166-5017EDU), transformation
A solution, transformation B solution,
C-growth medium, IPTG
BglII reagent refill pack
(#166-5018EDU), BglII restriction
enzyme, restriction enzyme buffer
Starter cultures are required for competent
cell preparation. Students will make LB agar
plates and streak them with E. coli bacteria
for single colonies. Students then prepare
LB broth to make an overnight starter culture
from their streaked plate, which is used to
prepare competent cells using the ligation
and transformation module.
LB ampicillin agar plates are required to
select for bacteria that have been transformed
with plasmids, like pJET1.2, which contain
the β-lactamase gene conferring ampicillin
resistance to bacteria. After transformation,
bacteria are plated onto LB ampicillin agar
plates and incubated at 37°C. Ampicillinresistant bacterial colonies will grow overnight.
LB broth containing ampicillin is required to
culture minipreps grown from colonies
transformed with ligated plasmid. Plasmids
are then isolated from the minipreps using
the Aurum™ plasmid mini purification kit.
On your own:
Teach basic microbiology with
all the reagents in one place. This kit
contains the reagents necessary for teaching
basic sterile technique, culturing E. coli,
transforming ampicillin-resistant bacteria, and
growing miniprep cultures. This module is
great for existing microbiology protocols and
independent study projects.
Kit supports 12 student workstations.
Microbial Culturing Module
Catalog #
List Price . . . . . . . . .EDU Price
166-5020EDU
$120.00 . . . . . . . . .$96.00
Ships and stores at room temperature.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Lab Preparation Checklist
Kit contains sufficient materials to pour 40 LB
agar plates (with or without ampicillin) and
inoculate and grow 75 miniprep cultures, as well
as starter E. coli bacteria.
Ampicillin, lyophilized
LB broth capsules
LB nutrient agar powder
Petri dishes, 60 mm, sterile
Cell culture tubes, 15 ml, sterile
Inoculation loops, sterile
E. coli strain HB101 K-12, lyophilized
Disposable plastic transfer pipets
Instruction manual
2
12
1
40
75
80
1
10
1
Required Accessories Not Included in Kit:
Microwave oven or autoclave
Recommended (Optional) Accessories:
Incubation oven, p. 137
Tube roller, p. 135
Shaking water bath or shaking incubator
1
1
1
CLASSROOM KITS: CLONING AND SEQUENCING EXPLORER SERIES
PCR Kleen™ Spin Purification Module:
As part of the Cloning and Sequencing
Explorer series
Refresh Kit Components: (more info pp. 84, 143)
Microbial culture kit reagent refill pack
(#166-5021EDU) includes ampicillin,
LB broth capsules, LB nutrient agar
powder, E. coli strain HB101 K-12
LB nutrient agar powder,
12.00
20 g (#166-0600EDU) or
500 g (#166-0472EDU)
89.00
Ampicillin (#166-0407EDU)
17.00
E. coli strain HB101 K-12
18.00
(#166-0408EDU)
Petri dishes, 60 mm, sterile, 500
128.00
(#166-0470EDU)
Inoculation loops, 10 µl, sterile, 80
13.00
(#166-0471EDU)
Disposable plastic transfer pipets,
105.00
sterile, 500 (#166-0474EDU)
Cell culture tubes, 17 x 100 mm, 14 ml, 17.00
sterile, 25 (#166-0476EDU)
Note: Bacterial culturing reagents such as
LB broth and LB agar are contained in the
microbial culturing module.
72
Visit us on the Web at explorer.bio-rad.com
73
Cloning and Sequencing Explorer Series: Cloning Pieces of the Puzzle
Isolate plasmid DNA from transformed
bacteria. Students inoculate minipreps with
transformed bacterial colonies from their agar
plates. They then isolate plasmid DNA using a
three-step alkaline lysis procedure followed by
mini column chromatography. Purification can
be carried out in microcentrifuges or using the
Aurum vacuum manifold.
Once plasmid DNA is eluted, students verify
insertion of the PCR product into the plasmid
vector using restriction enzyme digestion
analysis and agarose gel electrophoresis.
BglII restriction enzyme specific for screening
the pJET1.2 plasmid is supplied in the ligation
and transformation module. Students can
also determine plasmid DNA concentration
using spectrophotometry or fluorometry in
an optional step.
On your own:
Purify up to 20 µg of plasmid
DNA rapidly and inexpensively, without
the use of toxic reagents or alcohol
precipitations. Plasmid DNA is free of salts,
bacterial chromosomal DNA, and RNA.
The highly purified DNA makes it ideal for
subsequent molecular biology–based
applications such as automated sequencing,
cloning, PCR, or restriction digestion analysis.
Aurum Plasmid Mini Purification Module
Sequence and analyze your cloned DNA.
Students combine plasmid DNA containing
PCR products with sequencing primers on
a 96-well plate and send them to a
sequencing service.*
Kit supports 24 student workstations.
Aurum Plasmid Mini Purification Module
Catalog #
List Price . . . . . . . . .EDU Price
732-6400EDU
$217.14 . . . . . . . .$152.00
Ships and stores at room temperature.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Lab Preparation Checklist
Kit contains sufficient reagents to purify
DNA from 100 minicultures of plasmid-bearing
bacteria.
DNA-binding mini columns
Capless collection tubes
Resuspension solution, 25 ml
Lysis solution, 25 ml
Neutralization solution, 40 ml
5x wash solution, 25 ml
Elution solution, 16 ml
Instruction manual
100
100
1
1
1
1
1
1
Required Accessories Not Included in Kit:
95–100% lab grade ethanol, 100 ml
1
Microcentrifuge capable of greater
1
than 12,000 x g, p. 134
Shaking water bath or shaking incubator
1
(for growth of cultures)
Adjustable micropipets, p. 138
20–200 µl
1–12
100–1,000 µl
1–12
Pipet tips, p. 140
20–200 µl
1–12 boxes
100–1,000 µl
1–12 boxes
Recommended (Optional) Accessories:
Aurum vacuum manifold
(#732-6470EDU)
Sequencing and Bioinformatics Module:
As part of the Cloning and Sequencing
Explorer series
The portion of the GAPDH gene to be cloned
using the Cloning and Sequencing Explorer
series can vary from 0.6 to more than 2 kb in
length. To ensure complete coverage of the
gene, four sequencing primers are provided:
one forward and one reverse primer specific to
either side of the pJET1.2 cloning site, and one
forward and one reverse primer homologous to
different regions in the middle of the GAPDH
gene. Thus four independent sequences will
be generated for each plasmid. These
sequences can be assembled into a contig
using the bioinformatics module. We
recommend each student team submit two
plasmids for sequencing with all four primers.
The ultimate goal of the Cloning and
Sequencing Explorer series is to generate
sequence data that can be uploaded into
NCBI’s GenBank database to be used by
other scientists. To ensure the data are as
accurate as possible, it is important to guard
against sequencing errors by having as many
sequence reads as possible. Fortunately, since
this is a class project, it is probable that multiple
groups will independently clone the same
GAPDH gene, resulting in the desired repetition
needed to confirm the sequence. Additionally,
sequencing primers are provided to allow for
sequence reads in both forward and reverse
directions, providing another method of
sequence confirmation.
On your own:
1-800-4BIORAD (1-800-424-6723)
Kit supports 12 student workstations.
Sequencing and Bioinformatics Module
Catalog #
List Price . . . . . . . . .EDU Price
166-5025EDU
$327.50 . . . . . . . .$262.00
Ships on blue ice. Immediately store reagents bag at –20°C.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Lab Preparation Checklist
Kit contains sufficient materials for 12 student
workstations or 96 sequencing reactions.
pJETF primer, blue, 50 µl
pJETR primer, yellow, 50 µl
GAPSEQF primer, red, 50 µl
GAPSEQR primer, green, 50 µl
Control plasmid, 100 µl
Barcoded 96-well plate
Sealing film
Colored microcentrifuge tubes, 2 ml
Curriculum, including instruction manual
1
1
1
1
1
1
1
120
1
Bioinformatics: As part of the
Cloning and Sequencing Explorer series
Bioinformatics Module
Once the plasmid has been cloned and
sequenced, the real work begins —
interpreting the sequence data. This final
portion of the Cloning and Sequencing
Explorer Series teaches students essential
bioinformatics skills needed to link biology
with computers. The sequencing and
bioinformatics module includes a two
month subscription to your class’s personal
bioinformatics account. The account gives
students access to the latest tools in
bioinformatics data mining.
Raw sequence files are uploaded and
students are guided through a series of
activities. They will view and edit the original
chromatograms of their sequences, screen
out vector sequences, confirm their
sequences are GAPDH, perform NCBI
BLAST searches on their data to identify
and find related sequences, assemble their
forward and reverse sequences into contigs,
predict intron and exon structure, transcribe
and translate the GAPDH sequence and
compare it to known gene, mRNA, and
protein sequences.
Bioinformatics Module
This module serves the needs of educators
and students by combining bioinformatics
expertise in genetic analysis with Bio-Rad’s
expertise in wet-lab technology. With only
a computer, user name, and password,
teachers and students for the first time can
access over the Internet a state-of-the-art
commercial software system that supports
the process of scientific discovery.
Our bioinformatics provider has been
known around the globe for high-quality,
reliable software that helps customers
manage genetic data and further their
goals in DNA diagnostics and research and
in running laboratory-based businesses.
Used by laboratories and core facilities in
universities, government, biotechnology,
and pharmaceutical companies, these
bioinformatics software systems have
established an international reputation
for usability and performance.
Required Accessories Not Included in Kit:
Adjustable micropipets, p. 138
2–20 µl
1–12
Pipet tips, p. 140
2–20 µl
1–12 boxes
Sequencing service*
Computers and Internet access
1–12
Refresh Kit Components: (more info pp. 84, 143)
Sequencing module reagent refill
pack (#166-5026EDU), includes
pJETF primer, pJETR primer,
GAPSEQF primer, GAPSEQR
primer, control plasmid
On your own:
Sequence any PCR product
cloned into pJET1.2. The sequencing
primers specific for pJET1.2 will allow students
to sequence PCR fragments up to 1 kb
cloned into pJET1.2 using the ligation and
transformation module. Longer fragments
would require the design of internal
sequencing primers due to the limitations
of sequencing reads. Also, sequence the
pGAP positive control plasmid and use the
bioinformatics software to analyze the data.
74
Sequencing and Bioinformatics Module
CLASSROOM KITS: CLONING AND SEQUENCING EXPLORER SERIES
Aurum™ Plasmid Mini Purification
Module: As part of the Cloning and
Sequencing Explorer series
* Sequencing is not included. However,
in the U.S., Bio-Rad has partnered with
Eurofins MWG I Operon, a worldwide ISO9001
certified company ensuring high quality, in
order to offer a deeply discounted rate for
sequencing services to educators using the
Cloning and Sequencing Explorer series.
Eurofins MWG I Operon provides sequencing
data within 1–2 days. For more information
on other sequencing options, visit
www.operon.com/bio-rad or contact us
at [email protected]
Web-based tutorials will
provide free hands-on training with
the tools to make even a novice an expert.
Visit us on the Web at explorer.bio-rad.com
75
Classroom Kits: Protein Expression and Purification Series
Section Contents
Assessment Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Complete Protein Expression and Purification Series . . . . . . . . . . . . . . . . . 78
Move beyond DNA to the exciting world of proteins!
One of the great promises of the biotechnology industry is the ability to
produce biopharmaceuticals to treat human disease. Producing novel
proteins in bacteria or other cell types is not simple. Active proteins are
often composed of multiple chains of amino acids with complex folding
and strand interactions. Commandeering a particular cell to reproduce
the native form presents many challenges. Considerations of cell type,
plasmid construction, and purification strategy are all part of the process
of developing a recombinant protein.
In the Protein Expression and Purification series students will explore the
process of producing a recombinant protein by inducing E. coli to express
the protein of interest, dihydrofolate reductase (DHFR), which is a target
for certain cancer treatments. Students will learn how to recover the
protein from other cellular components and then purify it away from
other proteins in the cell using the leading form of purification today —
affinity chromatography.
The Biotechnology Explorer program has a long history of partnering
with educators to create laboratory experiences that prepare students
for today’s careers and provide the understanding that is required for
citizens in the rapidly advancing technologies that impact our
daily lives. The Protein Expression and Purification
series was developed in response to educators’
desire to provide students with authentic
protein-based laboratory experiences relevant
to research and industrial applications.
How can you teach these concepts
all at once?
• Protein purification (chromatography)
• Enzymatic analysis
• Research and development processes
• Biomanufacturing
• Cancer treatment targets
Growth and
Expression Module
Biotechnology Explorer makes it easy for you and your students
to experience each of the above concepts in an integrated
workflow. Teach the core process of expression and purification of
bioengineered proteins using this clear and concise modular lab
series. Help your students gain hands-on experience and give them
the confidence that they need to work with proteins. The modular
design of the new Protein Expression and Purification series allows
you to teach the basics of protein purification and then proceed to
the more advanced concepts.
76
1-800-4BIORAD (1-800-424-6723)
Growth and Expression Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
SDS-PAGE Electrophoresis Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Purification Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Option 1: Centrifugation Purification Module . . . . . . . . . . . . . . . . . . . . . . 81
Option 1
Centrifugation
Purification
Module
DHFR Enzymatic
Assay Module
Option 2: Hand-Packed Column Purification Module . . . . . . . . . . . . . . . 81
Option 3: Prepacked Cartridge Purification Module . . . . . . . . . . . . . . . . . 81
DHFR Enzymatic Assay Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Option 2
Purification
Module Module
Hand-Packed
Column
Purification
Module
“
Option 3
SDS-PAGE
Electrophoresis
Module
Prepacked
Cartridge
Purification
Module
My interaction with
Bio-Rad's technical
support specialist was
just wonderful! This is one
reason why Bio-Rad is
my number one favorite
science supply company.
I wish the others were
even a fraction as good
as Bio-Rad!
”
The scalability of this particular affinity purification process provides
an adaptable set of techniques and content to match the goals of the
beginning protein educator up to an advanced college-level course
in biomanufacturing. The series provides a fully developed and readyto-go lab course, including relevant background and protocols that
work. A module on student assessment is available as well. Discover
more about this unique series, including how DHFR is a target for
certain cancer treatments and how the protein expression and
purification process is vital to the world of biomanufacturing.
Assessment Module
This unique assessment guide provides ideas for using
formative assessment in your class to guide and increase
learning while students perform the lab activities. At the end
of the lab series use the summative assessment to evaluate
the final learning levels of
students. The assessment
tool is arranged according
to learning levels so that
you can choose what level
best evaluates the
comprehension of your
students.
CLASSROOM KITS: PROTEIN EXPRESSION AND PURIFICATION SERIES
Protein Expression and Purification Series . . . . . . . . . . . . . . . . . . . . . 76
Janet Hamilton
Instructor
Shenandoah Valley
Governor’s School
Fishersville, Virginia
77
Protein Expression and Purification Series: From Industrial Enzymes to Cancer Therapy —
The Complete Protein Expression and Purification
series centrifugation purification process supports
12 workstations while the chromatography
instrumentation hand-packed column and
prepacked cartridge processes each support
4 workstations.
Protein Expression and Purification
Series — All Four Modules (choose option)
Option 1: Centrifugation Purification Process
Catalog #
List Price . . . . . . . .EDU Price
166-5040EDU
$708.75 . . . . . . .$567.00
(for purification using a 16K microcentrifuge)
Option 2: Hand-Packed Column Process
166-5045EDU
897.50 . . . . . . . .718.00
(for use with chromatographic purification
instrumentation and allowing students to pour
their own columns)
Option 3: Prepacked Cartridge Process
166-5050EDU
897.50 . . . . . . . .718.00
(providing the best experience with
chromatographic purification instrumentation
through the quality of prepacked cartridges)
Assessment Module (optional)
166-5070EDU
62.50 . . . . . . . . .50.00
Ships with both temperature sensitive and room
temperature components. Immediately store temperature
sensitive items at 4°C or –20°C as indicated.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Workflow
The lab is presented over the next few
pages in a series representing the
experimental workflow:
1. Plate cells.
2. Inoculate.
3. Subculture, monitor, and induce.
4. Harvest and lyse cells.
5. Purify.
6. Measure purity and perform
enzymatic activity analysis.
Modules Available Separately:
Growth and Expression (#166-5055EDU)
SDS-PAGE Electrophoresis (#166-5060EDU)
Centrifugation Purification (#166-5041EDU)
Hand-Packed Column Purification (#166-5046EDU)
Prepacked Cartridge Purification (#166-5051EDU)
DHFR Enzymatic Assay (#166-5065EDU)
Assessment (#166-5070EDU)
Lab Preparation Checklist
Lab contains sufficient materials for 12 student
workstations when using the centrifugation
process or 4 student workstations when using
either the hand-packed column or prepacked
cartridge process.
78
1-800-4BIORAD (1-800-424-6723)
Growth and Expression Module:
As part of the Protein Expression and
Purification series
Lab Preparation Checklist (continued)
Protein Expression and Purification
Series Contents
Growth and Expression Module
SDS-PAGE Electrophoresis Module
Purification Module (one of the following)
Centrifugation Purification Process
Hand-Packed Column Purification Process
Prepacked Cartridge Purification Process
DHFR Enzymatic Assay Module
Instruction Manual
Assessment Module (available separately)
1
1
1
1
1
1
Required Accessories Not Included in Kit:
Dry ice
Ethanol
Adjustable micropipets, p. 138
Pipet tips, p. 140
Parafilm sealing film
UV compatible submicrovolume cuvettes, p. 131
1.5 ml standard disposable polystyrene or
quartz cuvettes, p. 131
10 ml syringes
22 gauge syringe needles
Fraction collection tubes
1 L Erlenmeyer flask or autoclavable bottle
Beakers for dry ice/ethanol bath
Microwave oven
–20°C freezer
Incubation oven, p. 137
Tube roller, p. 135
Mini rocker, p. 135
Dry bath or water bath, p. 136-137
UV/Vis spectrophotometer capable of reading
to three decimal places, p. 131
Pipet controller, p. 139
10 ml serological pipets
Power supply, p. 130
Vertical electrophoresis chambers, p. 120
Mini-PROTEAN® TGX™ polyacrylamide gels,
4–20%, 10-well, p. 123
Microcentrifuge with variable speed setting
(up to 16,000 x g), p. 134
Chromatography Instrumentation Process Only:
500 ml Erlenmeyer flasks
Shaking incubator or shaking water bath
capable of holding 4 x 500 ml flasks
Centrifuge capable of 16,000 x g with rotors
that hold 250 ml centrifuge bottles and
30–50 ml centrifuge tubes
Centrifuge bottles (250 ml) capable of
withstanding 4,500 x g
Centrifuge tubes (30–50 ml) capable of
withstanding 16,000 x g
Hand-Packed Column Process Only:
Flow adaptor, 1.0 cm column ID 1–7 cm
functional length (#738-0014EDU)
BioLogic DuoFlow™ System Only:
(#760-0047EDU or 760-0037EDU)
Fittings to convert luer to 1/4–28 (#732-0113EDU)
5 ml injection loop (#750-0497EDU)
Recommended (Optional) Accessories:
Autoclave
Vortexer, p. 135
Gel documentation system, p. 132
50 ml tube racks, set of 5 racks, p. 142
Refresh Kit Components:
Please visit explorer.bio-rad.com and download
bulletin 166-5067EDU for more information.
To be used for research, industrial, or
pharmaceutical purposes, proteins need to
be purified in large quantities. Some proteins
can be easily extracted from a readily available
source in large quantities. However, most
proteins are not naturally produced in a form
and in amounts that allow easy purification.
The techniques of genetic engineering
overcome the limitations of naturally produced
proteins by making cells synthesize specific
proteins in amounts that can be purified for
use in fundamental research or for industrial
and therapeutic applications.
The starting point in the Protein Expression
and Purification Series is lyophilized
BL21(DE3) E. coli containing pDHFR, which
will be rehydrated and plated to generate
individual colonies. An initial culture is grown
to saturation from a single colony. This culture
is used to initiate a larger culture that is grown
to mid-log phase, at which stage expression
of the recombinant protein is induced by
addition of IPTG to the medium. After
induction has been completed, cells are
recovered by centrifugation, and protein is
extracted from the cell pellet.
On your own:
Delve into protein expression
using the pDHFR plasmid and see how
glucose affects the lac operon system so that
no T7 RNA polymerase and subsequent
GST-DHFR-His is leaked. Explore the effects
of IPTG concentration on induction levels.
Growth and Expression Module
Kit supports 4–12 student workstations, depending
on the size of the cultures used.
Growth and Expression Module
Catalog #
List Price . . . . . . . . .EDU Price
166-5055EDU
$311.25 . . . . . . .$249.00
Ships at room temperature. Immediately store temperature
sensitive reagents bag at –20°C as indicated.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Lab Preparation Checklist
Kit contains sufficient materials for 4–12 student
workstations, depending on the size of cultures used.
2 ml EZ Micro™ test tubes
(microcentrifuge), clear
500
Petri dishes
20
Inoculation loops, sterile
20
Sterile conical tubes, 50 ml
25
Screwcap microcentrifuge tubes, 1.5 ml
50
Ampicillin, lyophilized
2
LB nutrient agar powder
1
LB broth capsules
12
Lysozyme, lyophilized
1
E. coli strain BL21(DE3) containing pDHFR,
lyophilized
1
IPTG, 1 M, 0.1 ml
1
10x PBS, 100 ml
1
Imidazole stock solution, 200 ml
1
20% sterile glucose, 4 ml
1
Sterile water, 2.5 ml
1
Instruction manual
1
CLASSROOM KITS: PROTEIN EXPRESSION AND PURIFICATION SERIES
Protein Expression and Purification Series
Protein Purification Is essential!
Required Accessories Not Included in Kit:
Adjustable micropipets, 2–1,000 µl, p. 138 1–12
Pipet tips, 2–1,000 µl, p. 140
1–12
Pipet controller, p. 139
1
10 ml serological pipets
Spectrophotometer, p. 131
1
Semi-microvolume cuvettes, p. 131
Water bath, p. 137
1
Incubator, p. 137
2–4
Tube roller, shaking water bath, or
shaking incubator p. 135
2–4
Autoclave, microwave, or hot plate and stir bar
Dry ice/ethanol
16K microcentrifuge, p. 134
1–12
10 ml syringes and 22 gauge syringe needles
Recommended (Optional) Accessories:
Vortexer, p. 135
1–12
Dry bath, p. 136
1–12
Refresh Kit Components: (more info pp. 84, 143)
Growth and Expression reagent refill
pack (#166-5057EDU) includes
ampicillin, LB agar, LB capsules, lysozyme,
BL21(DE3) with pDHFR, IPTG, sterile water,
10x PBS, imidazole stock solution, and
20% sterile glucose solution
Visit us on the Web at explorer.bio-rad.com
79
Protein Expression and Purification Series: From Industrial Enzymes to Cancer Therapy —
Purification Module: As part of the
Protein Expression and Purification series
SDS-PAGE Electrophoresis Module
In this lab, the induced expression of
GST-DHFR-His, solubility of the expressed
GST-DHFR-His, and success of purification
will be analyzed by SDS-PAGE.
Kit supports 12 student workstations.
SDS-PAGE Electrophoresis Module
Catalog #
166-5060EDU
On your own:
Save time and money running
SDS-PAGE gels using the highest
quality standards and buffers available.
Check out the long shelf life Mini-PROTEAN®
TGX™ precast gels that can run up to 30%
faster and have up to a 12-month shelf life.
Ships at room temperature. Precision Plus Protein™
Dual Color standards should be stored at –20°C. All
other reagents should be stored at room temperature.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Lab Preparation Checklist
Kit contains sufficient materials for 12 student
workstations.
10x Tris/glycine/SDS buffer, 1 L
Laemmli sample buffer, 30 ml
Bio-Safe™ Coomassie stain, 1 L
Precision Plus Protein Dual Color
standards, 500 µl
1
1
1
1
Required Accessories Not Included in Kit:
Vertical gel electrophoresis chambers, p. 120
Mini-PROTEAN TGX polyacrylamide gels,
4–20%, 10-well, p. 123
Water bath or dry bath, p. 136–137
Adjustable micropipets, p. 138
Pipet tips, p. 140
Power supplies, p. 130
Gel staining trays, 4 (#166-0477EDU) $16.00
Recommended (Optional) Accessories:
16K microcentrifuge, p. 134
Gel documentation system, p. 132
Protein purification is an important step
in biotechnology workflows. It is the
isolation of the protein of interest so that it may
be used in subsequent research, for diagnostic
tests, or for pharmaceutical production. The
purity needed depends on the protein’s end
use. For proteins used in research, 90–95%
purity may be sufficient, but for proteins used
for pharmaceutical applications, much higher
purity levels (up to 99.99%) must be reached.
How purification is done will depend on the type
of protein engineered, the volume of protein to
be purified, the degree of purity required, and
the availability of special laboratory equipment.
Each of these purification modules will allow
you to purify GST-DHFR-His using affinity
chromatography. Which module you choose
depends on the experience you want your
students to have and the equipment that you
have on hand. Choose the centrifugation
purification module to learn about spin column
chromatography using a 16K microcentrifuge.
If you have chromatographic purification
instrumentation such as Bio-Rad’s BioLogic™ LP
or BioLogic DuoFlow™ systems, then the handpacked purification and prepacked purification
modules are for you!
DHFR Enzymatic Assay Module:
As part of the Protein Expression and
Purification series
Three Purification Module Options
Kit supports 12 student workstations.
Option 1: Centrifugation Purification
Catalog #
166-5041EDU
List Price . . . . . . . .EDU Price
$67.50 . . . . . . . .$54.00
Kit supports 4 student workstations.
Option 2: Chromatography
Instrumentation Hand-Packed Column
Purification
166-5046EDU
Kit supports 4 student workstations.
Option 3: Prepacked Cartridge Purification
166-5051EDU
Each module ships at room temperature. Store at 4°C.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Lab Preparation Checklist
On your own:
For independent research
opportunities use these modules to
purify any polyhistidine tagged proteins.
See how each protein behaves differently and
has a different purification profile.
Dihydrofolate reductase (DHFR) is a critical
enzyme necessary for the conversion of
dihydrofolate (DHF) to tetrahydrofolate (THF).
This reaction also requires the presence of the
cofactor nicotinamide adenine dinucleotide
phosphate (NADPH). The concentration of
purified GST-DHFR-His will be calculated
using the proteins’ intrinsic absorbance of
UV light at 280 nm. This known concentration
of GST-DHFR-His will be combined with a
known quantity of NADPH, which absorbs at
340 nm. Since no DHF substrate is present,
the NADPH should not be reduced and the
absorbance at 340 nm should be constant
over time. Once the substrate DHF is added
to the solution containing the purified
GST-DHFR-His and NADPH, the absorbance
at 340 nm should decrease over time as the
reaction occurs, converting NADPH to NADP+
and DHF to THF.
On your own:
Measure the activity of any
DHFR enzyme with this module. Perfect
for use with independent research to determine
what happens if the DHFR source is changed
or the actual enzyme is mutated. What effects
will these changes have on DHFR activity?
DHFR Enzymatic Assay Module
Kits support 12 student workstations.
DHFR Enzymatic Assay Module
Catalog #
List Price . . . . . . . . .EDU Price
166-5065EDU
$111.25 . . . . . . . . .$89.00
Ships on blue ice. Immediately store at –20°C.
Educational discounts apply only to items
ordered with an EDU suffix. EDU price discounts
are for qualified educational institutions and
educators only.
Lab Preparation Checklist
Kit contains sufficient materials for 12 student
workstations.
Dihydrofolic acid substrate (DHF), 1 mg
NADPH, cofactor, 1 mg
1
1
Required Accessories Not Included in Kit:
Adjustable micropipets, p. 138
Pipet tips, p. 140
UV spectrophotometer capable of three decimal
place accuracy, p. 131
UV compatible cuvettes, p. 131
Parafilm
Recommended (Optional) Accessories:
Cuvette racks, set of 5 racks
(#166-0485EDU)
Kit contains sufficient materials for 4–12 student
workstations, depending on which process is used.
Centrifugation Purification Module
Profinity™ IMAC Ni-charged resin, 1.5 ml
Micro Bio-Spin™ empty columns
Micro Bio-Spin 6, Tris columns
Screwcap microcentrifuge tubes, 1.5 ml
Spin column wash tubes
CLASSROOM KITS: PROTEIN EXPRESSION AND PURIFICATION SERIES
SDS-PAGE Electrophoresis Module:
As part of the Protein Expression and
Purification series
Protein Purification Is essential!
1
12
12
50
25
Hand-Packed Column Purification Module
Profinity IMAC Ni-charged resin, 10 ml
1
1.0 cm x 5 cm, 2-pk glass Econo-Column™
columns
2
Micro Bio-Spin 6, Tris columns
25
Prepacked Cartridge Purification Module
4
Bio-Scale™ mini Profinity IMAC cartridge
Micro Bio-Spin 6, Tris columns
25
Required Accessories Not Included in Kit:
16K microcentrifuge, p. 134
Water bath or dry bath, pp.136-137
Adjustable micropipets and tips, pp. 138–140
Tube roller or rocker platform, p. 135
UV spectrophotometer, p. 131
UV compatible cuvettes, p. 131
BioLogic™ LP or BioLogic DuoFlow™ System,
p. 141 (required only for hand-packed column
and prepacked cartridge purification modules)
Recommended (Optional) Accessories:
Bench top centrifuge
$48.30
Cuvette racks, set of 5 racks
(#166-0485EDU)
80
1-800-4BIORAD (1-800-424-6723)
Visit us on the Web at explorer.bio-rad.com
81
Refresh Kit Components
Section Contents
Refresh Kit Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Refresh kit components reduce packaging waste, reuse
components, and refresh your kits while saving storage space
by letting you purchase individual items. Bio-Rad now has many
individual components for Biotechnology Explorer™ kits available
for purchase to help you maximize your space and budget
for the key items you need.
We’ve prepared guides to help you determine what you need for
your classes, including two different selection guides: a printable
Refresh Kit Components Purchasing Guide (bulletin 5872) and a
Refresh Kit Components Interactive Purchasing Guide (bulletin
REFRESH KIT COMPONENTS
Reduce. Reuse. Refresh. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
“
5905). Additionally, to help you with preparing LB agar plates and
agarose gels for large or multiple classes, we have created
I learned everything I know
about biotech using the Bio-Rad
equipment in my high school
program. The quality of the
equipment made a definite
impact on the lab interactions.
Being trained on the highest
quality equipment, whether
Bio-Rad pipets or thermal
cyclers, made a difference and
provided a distinct edge to start
my biotech career. The program,
the great instructor, and the best
equipment all provided me with
a real advantage and made a
difference in the quality of
scientist I am today.
our Large Class/Multiple Class Preparation Guide (bulletin 5891).
Visit us at explorer.bio-rad.com to learn more and to see our
helpful guides. Reduce. Reuse. Refresh.
1>
3>
”
2>
Craig Jerome
Santa Fe High School
Santa Fe Community College
Syngenta
Alachua, Florida
4>
5>
82
1. Mesa Community College, Mesa, Arizona; 2. Tennessee State University,
Nashville, Tennessee; 3. University of Tulsa, Tulsa, Oklahoma; 4. Bio-Rad Teacher
Workshop, Hyderabad, India; 5. University of Connecticut, Storrs, Connecticut.
83
Petri Dishes
•
Inoculation Loops
•
Jellyfish Foam Floating Racks
•
•
pGLO Kit SDS-PAGE Extension
Secrets of the Rainforest™ Kit
Microbes and Health Kit
Biofuel Enzyme Kit
Size Exclusion Chromatography Kit
Got Protein?™ Kit
ELISA Immuno Explorer™ Kit
Comparative Proteomics Kit I:
Protein Profiler Module
Comparative Proteomics Kit II:
Western Blot Module
Rapid Blotting — V3 Western Workflow™
Genes in a Bottle™ Kit
STEM Electrophoresis Kit
IDEA Electrophoresis Kit
Forensic DNA Fingerprinting Kit
Analysis of Precut Lambda DNA Kit
Restriction Digestion and Analysis
of Lambda DNA Kit
Crime Scene Investigator PCR Basics™ Kit
PV92 PCR Informatics Kit
Fish DNA Barcoding Kit
GMO Investigator™ Kit
Crime Scene Investigator PCR Basics Real-Time
PCR Starter Kit
GMO Investigator Real-Time PCR Starter Kit
Cloning and Sequencing Explorer Series
Protein Expression and Purification Series
pGLO™ Bacterial Transformation Kit
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Gel Staining Trays
Disposable Plastic
Transfer Pipets
(1 ml, graduated)
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Cell Culture Tubes
* Please refer to kit pages for TS and RT Reagent Refill Pack details.
84
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Description
Catalog No.
Reagent Refill Pack
*
*
*
pGLO Bacterial Transformation Kit Reagent Refill Pack
166-0555EDU
$73.75
$59.00
pGLO Plasmid, 20 µg, lyophilized
166-0405EDU
41.25
33.00
Arabinose, 600 mg, lyophilized
166-0406EDU
35.00
28.00
Ampicillin, 30 mg, lyophilized
166-0407EDU
21.25
17.00
E. coli Strain HB101 K-12, lyophilized
166-0408EDU
22.50
18.00
Transformation Solution, 15 ml
166-0409EDU
25.00
20.00
LB Nutrient Broth, 10 ml
166-0421EDU
21.25
17.00
LB Nutrient Agar Powder, 20 g
166-0600EDU
15.00
12.00
LB Nutrient Agar Powder, 500 g
166-0472EDU
111.25
89.00
Petri Dishes, 60 mm, sterile, 500
166-0470EDU
160.00
128.00
Inoculation Loops, 10 µl, sterile, 80
166-0471EDU
16.25
13.00
Jellyfish Foam Floating Racks, 8 racks
166-0479EDU
37.50
30.00
Long-Wave UV Lamp, 1
166-0500EDU
43.75
35.00
Long-Wave UV Pen Light, 1
166-0530EDU
13.75
11.00
50x TAE, 1 L
161-0743EDU
82.00
65.60
50x TAE, 5 L cube
161-0773EDU
235.00
188.00
Sample Loading Dye, 5x, 10 ml
161-0767EDU
20.00
16.00
Certified Molecular Biology Agarose, 25 g
161-3100EDU
59.00
47.20
Certified Molecular Biology Agarose, 125 g
161-3101EDU
169.00
135.20
Certified Molecular Biology Agarose, 500 g
161-3102EDU
543.00
434.40
Fast Blast™ DNA Stain, 500x, 100 ml
166-0420EDU
30.00
24.00
Small Fast Blast DNA Electrophoresis Reagent Pack
166-0450EDU
105.00
55.00
Medium Fast Blast DNA Electrophoresis Reagent Pack
166-0455EDU
270.00
189.00
Large Fast Blast DNA Electrophoresis Reagent Pack
166-0460EDU
700.00
560.00
UView 6x Loading Dye. 0.2 ml
166-5111EDU
31.25
25.00
UView 6x Loading Dye. 1 ml
166-5112EDU
95.00
76.00
Gel Staining Trays, 4
166-0477EDU
20.00
16.00
Genes in a Bottle DNA Extraction Reagent Refill Pack
166-2001EDU
50.00
40.00
Lysis Buffer, 110 ml
166-2002EDU
28.75
23.00
Helix DNA Necklace Module, 36 necklaces
166-2250EDU
82.50
66.00
Master Mix for PCR, 2x, 90 units, 1.2 ml
166-5009EDU
92.50
74.00
Disposable Plastic Transfer Pipets, sterile, 1 ml, 500
166-0474EDU
131.25
105.00
Disposable Plastic Transfer Pipets, nonsterile, 1 ml, 500
166-0480EDU
65.00
52.00
Colored 1.5 ml Microcentrifuge Tubes, 6 colors, 600
166-0473EDU
38.75
31.00
1.5 ml EZ Micro™ Test Tubes, clear, 500
223-9480EDU
29.00
23.20
2 ml EZ Micro Test Tubes, clear, 500
223-9430EDU
31.00
24.80
Conical Tubes, 1.5 ml, O-ring screw caps, sterile, 500
224-0110EDU
123.00
98.40
Conical Centrifuge Tubes, 15 ml, 50
166-0475EDU
32.50
26.00
Cell Culture Tubes, 17 x 100 mm, 14 ml, sterile, 25
166-0476EDU
21.25
17.00
0.2 ml Tubes with Domed Caps, clear, 1000
TWI-0201EDU
56.00
44.80
PCR Tube Capless Adaptors, 500
223-9500EDU
24.00
19.20
96-Place PCR-Tube Rack and Cover, set of 5, multicolored
TRC-0501EDU
38.00
30.40
™
•
•
•
1.5 ml Microcentrifuge
Tubes
2 ml
EZ Micro
•
•
List Price
EDU Price
1.5 ml
EZ Micro
500 µl
EZ Micro
REFRESH KIT COMPONENTS
LB Nutrient Agar Powder
C. elegans Behavior Kit
Refresh Kit Components: Reduce. Reuse. Refresh.
Green Racks
Storage Boxes
96-Place PCR-Tube Rack and Cover
15 ml Tube Rack
50 ml Tube Rack
Cuvette Rack
For more refresh items check out each individual kit page and the reagents and consumables pages throughout the catalog.
Visit us on the Web at explorer.bio-rad.com
85
Biotechnology: A Laboratory Skills Course — Textbook
Section Contents
Textbook . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Textbook Equipment Bundles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Textbook Kit Bundles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
“
BIOTECHNOLOGY: A LABORATORY SKILLS COURSE — TEXTBOOK
Biotechnology: A Laboratory Skills Course
The Bio-Rad textbook has
been an excellent resource
for planning for myself
and my students.
5>
”
Carol Robbins, PhD
Teacher, Biotechnology Program
University of Alabama at Birmingham
Birmingham, Alabama
4>
1>
86
2>
3>
6>
1. Acalanes High School, Lafayette, California; 2. NABT, Anaheim, California;
3. Shoreline Community College, Seattle, Washington; 4. Arizona State University,
Tempe, Arizona; 5. Bio-Link Teacher Workshop, Austin, Texas; 6. Jenks High
School, Jenks, Oklahoma.
87
Biotechnology: A Laboratory Skills Course — Textbook
Background sections for each
chapter include biological theory behind
the techniques and descriptions of the
techniques themselves.
Starting a biotechnology course has never been easier!
Biotechnology: A Laboratory Skills Course is a biotechnology textbook that is a
ready-to-go solution for your biotechnology course, allowing you to complement
your course or start a new one right away! This laboratory textbook provides you
and your students with background information about the methods and techniques
used in today’s exciting research and manufacturing laboratory environments, along
with real-world activities that allow your students to understand the powerful impact
science has on their everyday lives.
Essay questions follow the background section for each chapter and
act as starting points for independent
literature research beyond the textbook.
35 activities — non–kit-based and kit-based: Activities are an essential part
of engaging students to develop their skills, and provide the backbone for this
textbook. The non–kit-based activities, such as pipetting and solution making,
address core competencies needed in all areas of a molecular biology laboratory
or to update a general biology course.
Each student chapter includes: an introduction and background of fundamental
molecular biology concepts, real-world vignettes of careers, bioethics, key skills,
case studies, and hands-on activities that progressively build science proficiency.
Student Edition: 400-page combined hardcover textbook and lab manual includes
background and applications through laboratory protocols. The student edition provides
necessary biology review points, new skills descriptions, 30+ hands-on activities and a
guide for students to understand how they can be evaluated for skills proficiency.
ISBN# 978-0-9832396-0-4
Teacher Supplement: 192-page softcover book to be used in conjunction with
the student edition. It will guide you through setup and assessment of the activities.
It includes educator implementation steps, activity preparations, stopping points,
materials lists, skills assessment, answers to pre- and postlab questions, and pacing
and purchasing information. ISBN# 978-0-9832396-1-1
Table of Contents
Chapter 1: The Biotechnology Industry
Chapter 2: Laboratory Skills
Chapter 3: Microbiology and Cell Culture
Chapter 4: DNA Structure and Analysis
Chapter 5: Bacterial Transformation and
Plasmid Purification
Chapter 6: The Polymerase Chain Reaction
Chapter 7: Protein Structure and Analysis
Chapter 8: Immunological Applications
Chapter 9: Research Projects
Four types of
vignettes show
how biotechnology
concepts covered in
the chapter play a
role in our daily lives.
Vignette topics
include discussions
about bioethics,
careers, spotlights on
key skills, and real-life
case studies.
Chapter overview gives a road map of
subject matter covered in the book.
Activities implement the techniques
described in the background information.
Early activities focus on building basic
skills, while later activities use those basic
skills as a foundation for more advanced
techniques.
Laboratory skills are acquired by
performing the activity. The requirements
necessary to claim proficiency in those
skills are described in the Laboratory
Skills Assessment Rubric in Appendix E.
Teacher Edition: Two-book set includes one each of the Student Edition and Teacher
Supplement. New teaching resources developed by the author of Biotechnology: A
Laboratory Skills Course are now available in a DVD set included with the Teacher Edition.
Graphics illustrate the hands-on
activities to help students learn
techniques.
BIOTECHNOLOGY: A LABORATORY SKILLS COURSE — TEXTBOOK
Biotechnology: A Laboratory Skills Course Textbook
Step-by-step protocols lead
students through procedures and
provide guidance on results analysis.
The DVD is also sold separately. 30 videos provide classroom guidance for:
• Demonstrations of key techniques and highlights of important safety points
43 PowerPoint presentations provide classroom instruction for:
• Chapter backgrounds and textbook laboratory activities
Laboratory Notebook
• 200 pages (includes signature page, table of contents pages, and pages with gridlines
for record keeping)
• Preprinted spaces for proper documentation, control, and traceability
Whether you have a complete biotechnology course or wish to add a few biotech activities
to your life science class, our comprehensive resources provide ready-to-go support.
Ordering Information
Description
Catalog #
List Price
EDU Price
Research questions and objectives
outline the experiments.
Biotechnology: A Laboratory Skills Course Textbook
Teacher Edition (includes one each 166-1025EDU, 166-1026EDU, and 166-1052EDU)*
Student Edition (ISBN 978-0-9832396-0-4)
Teacher Supplement* (ISBN 978-0-9832396-1-1), teacher reference guide
Laboratory Notebook
Supplementary Materials DVD Set
166-1027EDU
166-1025EDU
166-1026EDU
166-1051EDU
166-1052EDU
$150.00
117.50
43.75
18.75
15.00
$120.00
94.00
35.00
15.00
12.00
* Teacher supplement contains guidance for the instructor, including lab prep, activity timelines, skills assessment, and pacing and purchasing guide.
EDU price discounts are for qualified educational institutions and educators only. Items are available at list price for noneducators (must be ordered without an EDU suffix).
88
Assessment rubrics help students
understand what is expected of them
and how to proficiently complete a task.
Prelab focus questions ensure
students’ understanding of the activity,
and postlab focus questions help
students analyze their results and
generate conclusions.
Visit us on the Web at explorer.bio-rad.com
89
Biotechnology: A Laboratory Skills Course — Textbook Equipment Bundles
•
Textbook Kit
166-1030EDU
166-1033EDU
Minimal
Equipment
Bundle*
Intro Biotech
Course Equipment
Bundle
Advanced Biotech
Course Equipment
Bundle
8 Classroom
1 Classroom
1 Classroom
8 Professional
8 Professional
8 Professional
8 Professional
8 Professional
8 Professional
PowerPac™ Basic Power Supply
1
2
2
Gel Staining Trays, 4
2
2
4
Jellyfish Foam Floating Racks, 8 racks
1
1
1
Green Racks, set of 5
2
2
2
Mini Incubation Oven
1
1
1
Temperature-Controlled Water Bath
1
1
1
Mini Centrifuge
1
2
2
Biotechnology Equipment Bundles
Micropipetors
Adjustable -Volume Digital Micropipet, 2–20 µl
Adjustable -Volume Digital Micropipet, 20–200 µl
Adjustable -Volume Digital Micropipet, 100–1,000 µl
Mini-Sub® Cell GT Electrophoresis Chamber
with 7 x 10 cm tray and two 8-well combs
4
4
4
Racked Pipet Tips
TBR-35 tips, 2–200 µl, 1,000
TBR-40 tips, 100 –1,000 µl, 1,000
Xcluda™ B tips, 2–20 µl, 960
Xcluda D tips, 20 –200 µl, 960
Xcluda E tips, 100 –1,000 µl, 960
2
1
—
—
—
2
1
1
1
1
2
1
1
1
1
Long-Wave UV Lamp, requires 4 AA batteries
—
8
8
Tube Roller
—
1
1
PCR Tube Racks, set of 5
—
2
2
T100™ Thermal Cycler
—
1
1
GelAir™ Drying System
—
—
1
—
—
4
Storage Boxes, set of 5
—
—
2
Digital Dry Bath
—
—
1
DyNA Chill
—
—
8
Model 16K Microcentrifuge
—
—
1
PCR Tube Adaptor for 16K Microcentrifuge
—
—
1
UltraRocker™ Rocking Platform
—
—
1
SmartSpec™ Plus Spectrophotometer
—
—
1
—
—
4
Mini Trans-Blot Module
* The Minimal Equipment bundle, 166-1039EDU, comes with the classroom series micropipetors.
Packages are subject to change; check with Bio-Rad for the most up-to-date purchasing information. For 220/230 V packages contact your local Bio-Rad representative or office.
90
166-1043EDU
166-1044EDU
Minimal
Equipment
Bundle
with Textbooks
Intro Biotech
Course Equipment
Bundle
with Textbooks
Advanced Biotech
Course Equipment
Bundle
with Textbooks
Student Edition
32
32
32
Teacher Edition
1
1
1
166-1036EDU
166-1037EDU
166-1038EDU
Minimal
Equipment Kit
Bundle
Intro Biotech
Course Kit
Bundle
Advanced Biotech
Course Kit
Bundle
1
1
1
Biotechnology Equipment
with Textbook Bundles
Biotechnology: A Laboratory Skills Course Textbooks
Biotechnology Kit Bundles
Biofuel Enzyme Kit
™
Mini-PROTEAN® Tetra Cell System
®
166-1042EDU
ELISA Immuno Explorer Kit
1
1
1
Forensic DNA Fingerprinting Kit
1
1
—
Genes in a Bottle™ Kit
1
1
1
Got Protein?™ Kit
1
1
—
Microbes and Health Kit
1
1
1
pGLO™ Bacterial Transformation Kit
1
1
1
Restriction Digestion and Analysis of Lambda DNA Kit
1
1
1
Size Exclusion Chromatography Kit
1
1
—
Crime Scene Investigator PCR Basics™ Kit
plus small DNA electrophoresis reagent pack
—
1
—
GMO Investigator™ Kit
plus small DNA electrophoresis reagent pack
—
1
1
Aurum™ Plasmid Mini Purification Module
—
—
1
Comparative Proteomics Kit I: Protein Profiler Module
—
—
1
Comparative Proteomics Kits I and II: Protein Profiler
and Western Blot Module
—
—
1
Green Fluorescent Protein Chromatography Kit
—
—
1
Microbial Culturing Module
—
—
1
PV92 PCR Informatics Kit
—
—
1
BIOTECHNOLOGY: A LABORATORY SKILLS COURSE — KIT AND EQUIPMENT BUNDLES
166-1039EDU
Bundles
Ordering Information
Catalog #
List Price
EDU Price
Biotechnology Equipment Bundles
Minimal Equipment Bundle
Intro Biotech Course Equipment Bundle
Advanced Biotech Course Equipment Bundle
Description
166-1039EDU
166-1030EDU
166-1033EDU
$5,333.34
17,333.33
34,000.00
$4,000.01
13,000.00
25,500.00
Biotechnology Equipment with Textbook Bundles
Minimal Equipment Bundle with 32 Student Editions + 1 Teacher Edition
Intro Biotech Course Equipment Bundle with 32 Student Editions + 1 Teacher Edition
Advanced Biotech Course Equipment Bundle with 32 Student Editions + 1 Teacher Edition
166-1042EDU
166-1043EDU
166-1044EDU
9,333.34
21,333.33
36,660.00
7,000.01
16,000.00
27,495.00
Biotechnology Kit Bundles
Minimal Equipment Kit Bundle
Intro Biotech Course Kit Bundle
Advanced Biotech Course Kit Bundle
166-1036EDU
166-1037EDU
166-1038EDU
1,312.50
1,812.50
2,625.00
1,050.00
1,450.00
2,100.00
Visit us on the Web at explorer.bio-rad.com
91
Instructional Laboratory: Equipment Packages
Section Contents
DNA and Protein Electrophoresis Lab Packages . . . . . . . . . . . . . . . . . . . . 94
Biotech Instructional Starter Packages . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
PCR Instructional Lab Packages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Complete Biotechnology Instructional Lab Package . . . . . . . . . . . . . . . . . . 98
Advanced Biotechnology Lab Package . . . . . . . . . . . . . . . . . . . . . . . . . . 100
“
4>
Bio-Rad Labs have been
instrumental in supporting the
content in my teaching. They
deliver unique experiences that
resonate with students and
their understanding of various
biotech topics. The kits are so
well organized, easy to use,
and self-contained, which
allows my classes to do many
more interesting investigations
than would be possible without
Bio-Rad as a resource.
INSTRUCTIONAL LABORATORY EQUIPMENT PACKAGES
Instructional Laboratory Equipment Packages . . . . . . . . . . . . . . . . . . 92
”
Kristine Corolis
2013 Outstanding Biology Teacher Award
Biology Teacher
Ridley College
St. Catharines, ON, Canada
3>
1>
92
2>
5>
1. Rodriguez High School, Fairfield, California; 2. Bio-Rad Teacher Workshop,
Hot Springs, Arkansas; 3. Palmer High School, Palmer, Alaska; 4. Sacred Heart
High School, San Francisco, California; 5. Oral Roberts University, Tulsa, Oklahoma.
93
DNA Electrophoresis: Instructional Laboratory Equipment Packages
Protein Electrophoresis: Instructional Laboratory Equipment Packages
INSTRUCTIONAL LABORATORY EQUIPMENT PACKAGES
*
*
DNA Electrophoresis Instructional Lab Set 1
Build up your biotech lab step by step with research-quality electrophoresis lab sets.
Provide the very best molecular biology experiences for your students, year after year.
Add modules as your program grows.
Includes:
1 PowerPac™ Mini power supply
4 Classroom digital micropipets, 2–20 µl range*
2 Mini-Sub® cell GT systems — each system includes gel tank with easy-to-replace
electrode cassettes, lid with 3-ft power leads, 7 x 10 cm UV-transparent gel tray with
fluorescent ruler, and two 8-well fixed-height drop-in combs for ease in casting gels
* Varies depending on package chosen.
Protein Electrophoresis Instructional Lab Set 1
DNA electrophoresis
instructional lab set 1
supports a classroom laboratory
of up to 16 students and
will run all of the following
Biotechnology Explorer™ kits:
Includes:
1 PowerPac™ Mini power supply
4 Classroom digital micropipets, 2–20 µl range*
2 Mini-PROTEAN® Tetra cells for Ready Gel® precast gels; 2-gel system includes
electrode assembly, tank, lid with power cables, mini cell buffer dam
2 10-well gel loading guides
• Analysis of precut lambda DNA kit
(#166-0001EDU)
* Varies depending on package chosen.
• Forensic DNA fingerprinting kit
(#166-0007EDU)
Ordering Information
Ordering Information
Description
DNA Electrophoresis Instructional Lab Set 1 (120 V)
DNA Electrophoresis Instructional Lab Set 1 (120 V)
with upgraded professional digital micropipets
Description
Catalog #
166-0705EDU
166-0700EDU
List Price
$1,665.00
2,240.00
EDU Price
$999.00
1,680.00
Protein electrophoresis
instructional lab set 1
supports a classroom laboratory
of up to 16 students and
will run all of the following
Biotechnology Explorer™ kits:
• Restriction digestion and analysis
of lambda DNA kit (#166-0002EDU)
Protein Electrophoresis Instructional Lab Set 1 (120 V)
Protein Electrophoresis Instructional Lab Set 1 (120 V)
with upgraded professional digital micropipets
• IDEA kit (#166-5075EDU)
Catalog #
List Price
EDU Price
166-0825EDU
166-0810EDU
$1,650.00
2,400.00
$1,155.00
1,800.00
• Comparative proteomics kit I:
protein profiler module
(#166-2700EDU)
• pGLO™ kit SDS-PAGE extension
(#166-0013EDU)
This package is not available outside the U.S.
This package is not available outside the U.S.
*
*
DNA Electrophoresis Instructional Lab Set 2
Includes:
1 PowerPac Basic power supply
8 Classroom digital micropipets, 2–20 µl range*
4 Mini-Sub cell GT systems — each system includes gel tank with easy-to-replace
electrode cassettes, lid with 3 foot power leads, 7 x 10 cm UV-transparent gel tray with
fluorescent ruler, and two 8-well fixed-height drop-in combs for ease in casting gels
DNA electrophoresis
instructional lab set 2
supports a classroom laboratory
of up to 32 students and
will run all of the following
Biotechnology Explorer kits:
• Analysis of precut lambda DNA kit
(#166-0001EDU)
* Varies depending on package chosen.
• Forensic DNA fingerprinting kit
(#166-0007EDU)
Ordering Information
Description
DNA Electrophoresis Instructional Lab Set 2 (120/220 V)
DNA Electrophoresis Instructional Lab Set 2 (120/220 V)
with upgraded professional digital micropipets
Catalog #
List Price
EDU Price
166-0706EDU
166-0701EDU
$2,857.14
4,000.00
$2,000.00
3,000.00
For 220/230 V packages contact your local Bio-Rad representative or office.
94
• Restriction digestion and analysis
of lambda DNA kit (#166-0002EDU)
• IDEA kit (#166-5075EDU)
Protein Electrophoresis Instructional Lab Set 2
Includes:
1 PowerPac Basic power supply
8 Classroom digital micropipets, 2–20 µl range*
4 Mini-PROTEAN Tetra cells for Ready Gel precast gels; 2-gel system includes
electrode assembly, tank, lid with power cables, mini cell buffer dam
4 10-well gel loading guides
Protein electrophoresis
instructional lab set 2
supports a classroom laboratory
of up to 32 students and
will run all of the following
Biotechnology Explorer kits:
* Varies depending on package chosen.
Ordering Information
Description
Protein Electrophoresis Instructional Lab Set 2 (120/220 V)
Protein Electrophoresis Instructional Lab Set 2 (120/220 V)
with upgraded professional digital micropipets
Catalog #
List Price
EDU Price
166-0826EDU
166-0820EDU
$3,200.00
4,640.00
$2,240.00
3,480.00
• Comparative proteomics kit I:
protein profiler module
(#166-2700EDU)
• pGLO kit SDS-PAGE extension
(#166-0013EDU)
For 220/230 V packages contact your local Bio-Rad representative or office.
Visit us on the Web at explorer.bio-rad.com
95
Biotechnology: Instructional Laboratory Equipment Packages
PCR: Instructional Laboratory Equipment Packages
*
*
Biotech Instructional Starter Package
Build up your biotechnology laboratory step by step with research-quality lab sets. Provide the
very best molecular biology experiences for your students, year after year. Add modules as your
program grows. For 220/230 V packages contact your local Bio-Rad representative or office.
Includes:
2 PowerPac™ Basic power supplies
1 Mini incubation oven
1 Water bath
1 UltraRocker™ rocking platform
8 UV lamps
2 Mini centrifuges
8 50 µl fixed-volume pipets
9 Classroom digital micropipets, 2– 20 µl*
1 Classroom digital micropipet, 20 –200 µl*
1 Classroom digital micropipet, 100 –1,000 µl*
4 Mini-Sub® cell GT electrophoresis cells
(each cell includes one 7 x 10 cm tray and two 8-well combs)
10 Racks pipet tips (2–200 µl), 200 tips/rack
10 Racks pipet tips (100 –1,000 µl), 100 tips/rack
1 package agarose gel support film, 50
• Genes in a Bottle™ kit
(#166-2300EDU)
• pGLO™ bacterial transformation kit
(#166-0003EDU)
• Green fluorescent protein
chromatography kit (#166-0005EDU)
• Secrets of the Rainforest™ kit
(#166-0006EDU)
• Size exclusion chromatography kit
(#166-0008EDU)
• Analysis of precut lambda DNA kit
(#166-0001EDU)
Catalog #
List Price
EDU Price
166-0755EDU
166-0756EDU
$8,214.29
9,600.00
$5,750.00
7,200.00
For 220/230 V packages contact your local Bio-Rad representative or office.
96
Build up your PCR lab step by step with research-quality lab sets. Provide the very best
molecular biology experiences for your students, year after year. Add modules as your
program grows. For 220/230 V packages contact your local Bio-Rad representative or office.
PCR instructional lab
package supports a classroom
laboratory of up to 32 students,
includes one instructor’s set of
micropipets, and will run all of
the following Biotechnology
Explorer™ kits:
Includes:
1 T100™ thermal cycler*
1 PowerPac™ Basic power supply
2 Mini centrifuges
1 Water bath
9 Classroom digital micropipets, 2–20 µl*
1 Classroom digital micropipet, 20–200 µl*
1 Classroom digital micropipet, 100 –1,000 µl*
4 Mini-Sub® cell GT electrophoresis cells
(each cell includes one 7 x 10 cm tray and two 8-well combs)
• Crime Scene Investigator
PCR Basics™ kit (#166-2600EDU)
• GMO Investigator™ kit
(#166-2500EDU)
* Varies depending on package chosen.
• PV92 PCR informatics kit
(#166-2100EDU)
• Analysis of precut lambda
DNA kit (#166-0001EDU)
• Forensic DNA fingerprinting kit
(#166-0007EDU)
Ordering Information
Biotech Instructional Starter Package
Biotech Instructional Starter Package with upgraded
professional digital micropipets
PCR Instructional Lab Package
• ELISA Immuno Explorer™ kit
(#166-2400EDU)
* Varies depending on package chosen.
Description
Biotech instructional starter
package supports a classroom
laboratory of up to 32 students,
includes one instructor’s set of
micropipets, and will run all of
the following Biotechnology
Explorer™ kits:
• Restriction digestion and analysis of
lambda DNA kit (#166-0002EDU)
• IDEA kit (#166-5075EDU)
INSTRUCTIONAL LABORATORY EQUIPMENT PACKAGES
*
• Restriction digestion and
analysis of lambda DNA kit
(#166-0002EDU)
Ordering Information
Description
PCR Instructional Lab Package
PCR Instructional Lab Package with upgraded professional
digital micropipets and C1000 Touch™ thermal cycler
Catalog #
List Price
EDU Price
166-0765EDU
166-0766EDU
$8,800.00
15,200.00
$6,600.00
11,400.00
• Forensic DNA fingerprinting kit
(#166-0007EDU)
• IDEA kit (#166-5075EDU)
For 220/230 V packages contact your local Bio-Rad representative or office.
Visit us on the Web at explorer.bio-rad.com
97
Complete: Instructional Laboratory Equipment Packages
INSTRUCTIONAL LABORATORY EQUIPMENT PACKAGES
Complete Biotechnology Instructional
Lab Package
This equipment package supports all Biotechnology Explorer™
kits and most biotechnology and molecular biology laboratory
applications.
Includes:
4 Mini centrifuges
1 T100™ thermal cycler*
1 UltraRocker™ rocking platform
1 Incubation oven
1 Water bath
1 GelAir™ dryer
1 Package GelAir cellophane, 50
1 GelAir assembly table
2 GelAir drying frames (+16 clamps)
1 BR-2000 vortexer
2 PowerPac™ Basic power supplies
4 Mini-Sub® cell GT electrophoresis chambers
(each cell includes one 7 x 10 cm tray and
two 8-well combs)
4 Mini-PROTEAN® Tetra cell 2-gel systems for
TGX™ precast gels
4 Mini Trans-Blot® modules
4 10-well gel loading guides
8 UV lamps
1 Package agarose gel support film, 50
9 Professional adjustable-volume micropipets, 2–20 µl
9 Professional adjustable-volume micropipets, 20 –200 µl
1 Professional adjustable-volume micropipet, 100 –1,000 µl
10 Racks pipet tips (2–200 µl), 200 tips/rack
10 Racks pipet tips (100 –1,000 µl), 100 tips/rack
5 Racks Prot/Elec™ pipet tips (1–200 µl),
200 tips/rack
*
* Varies depending on package chosen.
1,000
1–200 µl pipet tips
2,000
2–200 µl pipet tips
1,000
100–1,000 µl pipet tips
Complete biotechnology
instructional lab package
supports 32 students and includes
one instructor’s set of micropipets.
Package will run most
Biotechnology Explorer classroom
lab kits (pp. 14–63).
20–200 µl
Ordering Information
Description
Catalog #
Research Package
List Price
EDU Component
List Price
EDU Package
Price
Complete Biotechnology Instructional Lab (120 V)
166-8005EDU
$22,400.00
$18,656.50
$16,800.00
Complete Biotechnology Instructional Lab (120 V) with upgraded
C1000 Touch™ thermal cycler (dual 48/48 heat block)
166-8008EDU
27,200.00
21,962.50
20,400.00
100 –1,000 µl
2–20 µl
This package is not available outside the U.S.
98
Visit us on the Web at explorer.bio-rad.com
99
Advanced: Instructional Laboratory Equipment Packages
*
*
This equipment package supports all Biotechnology Explorer™ kits and
most biotechnology and molecular biology laboratory applications.
Includes:
4 Mini centrifuges
1 T100™ thermal cycler*
1 UltraRocker™ rocking platform
1 Incubation oven
1 Water bath
1 Mini rocker
1 Tube roller
1 Digital dry bath
1 GelAir™ dryer
1 Package GelAir cellophane, 50
1 GelAir assembly table
2 GelAir drying frames (+16 clamps)
1 Package agarose gel support film, 50
1 BR-2000 vortexer
2 PowerPac™ Basic power supplies
8 Mini-Sub® cell GT electrophoresis chambers
(each cell includes one 7 x 10 cm tray and two 8-well combs)
8 Mini-PROTEAN® Tetra cell 2-gel systems for TGX™ precast gels
8 Mini Trans-Blot® modules
8 10-well gel loading guides
1 SmartSpec™ Plus spectrophotometer
1 Gel Doc™ EZ imaging system*
1 iMark™ microplate reader with Microplate Manager® software
1 MicroPulser™ electroporator
1 16K microcentrifuge
8 UV lamps
8 Professional adjustable-volume micropipets, 0.5 –10 µl
8 Professional adjustable-volume micropipets, 2–20 µl
8 Professional adjustable-volume micropipets, 20 –200 µl
8 Professional adjustable-volume micropipet, 100 –1,000 µl
10 Racks pipet tips (2–200 µl), 200 tips/rack
10 Racks pipet tips (100 –1,000 µl), 100 tips/rack
5 Racks Prot/Elec™ pipet tips (1–200 µl), 200 tips/rack
8 DyNA Chill coolers
2 Green racks, set of 5 racks
2 Storage boxes, set of 5 boxes, multicolored
2 96-Place PCR-Tube rack and cover, set of 5
1,000
1–200 µl pipet tips
0.5 –10 µl
2– 20 µl
20–200 µl
2,000
2–200 µl pipet tips
INSTRUCTIONAL LABORATORY EQUIPMENT PACKAGES
Advanced Biotechnology Lab Package
1,000
100–1,000 µl pipet tips
100–1,000 µl
* Varies depending on package chosen.
Ordering Information
Description
Catalog #
Research Package
List Price
EDU Component
List Price
EDU Package
Price
Advanced Biotechnology Lab (120 V) with easy-to-use Gel Doc EZ imaging
system with white light and UV sample trays
166-8021EDU
$60,000.00
$50,692.53
$45,000.00
Advanced Biotechnology Lab (120 V) with upgraded C1000 Touch™ thermal
cycler (dual 48/48 heat block) and easy-to-use Gel Doc EZ imaging
system with white light and UV sample trays
166-8022EDU
64,000.00
53,998.53
48,000.00
Advanced Biotechnology Lab (120 V) with Molecular Imager® Gel Doc™ XR+ system
166-8010EDU
$62,400.00
$58,252.53
$46,800.00
Advanced Biotechnology Lab (120 V) with upgraded C1000 Touch thermal
cycler (dual 48/48 heat block) and Molecular Imager Gel Doc XR+ system
166-8020EDU
66,400.00
61,558.53
49,800.00
Advanced biotechnology lab
package supports 32 students.
Package will run all Biotechnology
Explorer classroom lab kits including
the Cloning and Sequencing Explorer
Series and Protein Expression and
Purification Series (centrifugation
purification process); supports
independent research projects
(pp. 14– 63 and 66–81).
This package is not available outside the U.S.
100
Visit us on the Web at explorer.bio-rad.com
101
DNA and Genomic Studies: Equipment and Reagents
Section Contents
Horizontal DNA Electrophoresis Gel Boxes . . . . . . . . . . . . . . . . . . . . . . . 104
DNA Electrophoresis Reagent Packs . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
Agarose Gel Drying Film and Agarose Powders . . . . . . . . . . . . . . . . . . . . 106
DNA Electrophoresis Buffers and Reagents . . . . . . . . . . . . . . . . . . . . . . . 107
Precast Agarose Gels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
DNA Electrophoresis Size Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
DNA Stains . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
PCR and Gene Transfer
PCR Thermal Cyclers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
PCR Plastics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Real-Time PCR Detection Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Amplification/PCR Reagents and Gene Transfer . . . . . . . . . . . . . . . . . . . 114
Cell Counting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Nucleic Acid Purification
Genomic DNA, RNA, and Plasmid Purification Kits . . . . . . . . . . . . . . . . . 116
DNA Model
DNA Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
DNA AND GENOMIC STUDIES: EQUIPMENT AND REAGENTS
Horizontal DNA Electrophoresis
“
3>
Students learn best
when they have the
opportunity to see
practical applications of
the concepts they study.
Bio-Rad products afford
students the experience
of engaging in the
practice of science.
”
2>
Narsh Ramrattan
2013 Outstanding Biology Teacher Award
Vice Principal, Chinook’s Edge School Division
Innisfall, AB, Canada
1>
102
4>
1. Tulsa Community College, Tulsa, Oklahoma; 2. Austin Community College,
Austin, Texas; 3. Bio-Rad Teacher Workshop, Waipahu, Hawaii; 4. NSTA,
San Francisco, California.
103
DNA and Genomic Studies: Horizontal DNA Electrophoresis Gel Boxes
Mini-Sub Cell GT Cell Features
Agarose gel electrophoresis is a primary procedure in molecular biology. Designed
for research, our Mini-Sub cell GT gel boxes are the real thing.
Safety lid
Teaching applications include:
• IDEA kit, pp. 48– 49
• Lambda DNA restriction digestion and analysis kits, pp. 52– 53
• Forensic DNA fingerprinting kit, pp. 50– 51
• Crime Scene Investigator PCR Basics™ kit, pp. 56–57
• PV92 PCR informatics kit, pp. 58–59
• Fish DNA barcoding kit, pp. 60– 61
• GMO Investigator™ kit, pp. 62– 63
• Cloning and Sequencing Explorer series, pp. 66– 75
Fixed-height
combs
Comb
slots
UV-transparent
gel tray
Fluorescent
ruler
Gel
casting
gates
Removable
electrode
cassettes
Leveling
feet
Easy-to-clean electrodes —
QuickSnap electrodes are easy to remove
and simplify cleaning
Leakproof system — new electrode
design prevents buffer leaks from the base
104
The wide Mini-Sub cell GT cell provides a larger (15 cm) platform to handle more
samples. Using the 15- or 20-well combs, this cell has two to four times more
sample capacity than the Mini-Sub cell. Put a whole class on one gel!
ReadySub-Cell™ GT Cells
ReadySub-Cell GT gel boxes are similar to our Mini-Sub cell GT cells but are dedicated
to running ReadyAgarose™ precast gels (p. 107). ReadyAgarose gel trays lock into the
ReadySub-Cell GT chambers so that gels will not move or float during electrophoresis
runs. Available in both the mini and wide mini sizes, ReadySub-Cell systems used with
ReadyAgarose gels offer economy, consistency, and the utmost convenience.
Wide Mini-Sub Cell GT Cell
Additional supplies:
• Power supplies, pp. 130
• DNA electrophoresis buffers, p. 106–107
• DNA size standards, p. 108
Ordering Information
Catalog #
List Price
EDU Price
170-4487EDU
166-4000EDU
166-4288EDU
166-4270EDU
$299.00
357.14
408.57
415.72
$239.20
250.00
286.00
291.00
Mini-Sub Cell Accessories
Mini-Gel Caster, for Mini-Sub and wide Mini-Sub Cell trays
Mini-Sub Cell GT Casting Gates, for 7 x 7 cm tray only, 2
UV-Transparent Gel Tray, 7 x 7 cm
UV-Transparent Gel Tray, 7 x 10 cm
Fixed-Height Comb, 8-well, 1.5 mm
Fixed-Height Comb, 15-well, 1.5 mm
170-4422EDU
170-4434EDU
170-4436EDU
170-4435EDU
170-4463EDU
170-4465EDU
84.00
89.00
55.00
54.00
55.00
55.00
67.20
62.30
38.50
37.80
38.50
38.50
Wide Mini-Sub Cell GT Systems
Wide Mini-Sub Cell GT Cell, with 15 x 10 cm tray, 15-well and 20-well combs
Wide Mini-Sub Cell GT Cell, with 15 x 10 cm tray, 15-well and 20-well combs, gel caster
170-4468EDU
170-4469EDU
509.00
556.00
407.20
444.80
Environmentally friendly system —
less plastic is used in manufacturing the
redesigned model and results in a more
durable system
Wide Mini-Sub Cell Accessories
UV-Transparent Gel Tray, 15 x 7 cm
UV-Transparent Gel Tray, 15 x 10 cm
Fixed-Height Comb, 15-well, 1.5 mm
Fixed-Height Comb, 20-well, 1.5 mm
170-4426EDU
170-4416EDU
170-4446EDU
170-4448EDU
80.00
91.00
55.00
55.00
56.00
63.70
38.50
38.50
Safety certification — all Bio-Rad
electrophoresis equipment is IEC 1010-1
certified. You are assured that our gel
boxes are the safest you can buy!
Mini-Sub cell and wide Mini-Sub cell GT systems include: cell tank and lid (gel box), casting accessories as specified above, leveling bubble, one–two 1.5 mm fixed-height
combs as specified (8-well for the Mini-Sub cell, 15- and 20-well for the wide Mini-Sub cell), and a UV-transparent gel tray. Please order power supplies, additional trays,
combs, and accessories as needed. For complete details about Bio-Rad gel boxes and accessories for electrophoresis, visit explorer.bio-rad.com and request bulletin 2660.
Simple assembly — color-coded, labeled
electrodes and labeled base guarantee
correct positioning of the lid on the base
This gel shows the
results from the PV92
PCR informatics kit (page 58).
Following a 30 minute run at
100 V, the agarose gel was
stained with our unique and
nontoxic Fast Blast™ DNA stain.
See p. 109 for details on this DNA stain.
Mini-Sub Cell GT Cell
Mini-Sub Cell GT Systems
Mini ReadySub-Cell GT Cell, for precast ReadyAgarose gels, tank and lid only
Mini-Sub Cell GT Cell, with 7 x 10 cm tray, two 8-well combs (no casting gates)
Mini-Sub Cell GT Cell, with 7 x 10 cm tray, two 8-well combs, mini-gel caster
Mini-Sub Cell GT Cell, with 7 x 7 cm tray, one 8-well comb, casting gates
Intuitive setup — arrow on the side of
the base indicates the direction of the
run, ensuring proper gel orientation
Mini-Sub Cell GT Cell
with optional casting gates
and Mini-Gel Caster
Mini-Sub and Wide Mini-Sub Cell GT Cells
Fast and flexible, the Mini-Sub cell GT cell is our most popular classroom gel box.
The Mini-Sub cell GT cell will hold either a 7 x 7 cm or a 7 x 10 cm gel tray. A
10 cm gel poured with two 8-well combs (see photo, p. 104) easily accommodates
two student teams at a time. At 100 V, this cell resolves DNA fragments from
20 to 20,000 base pairs in just under 30 minutes (or for faster 20 minute gels see
bulletin 5396). Save time and money!
Easy lid removal — longer tabs on the
base prevent incorrect lid positioning and
enable easy removal of the lid, reducing
buffer spillage
Flexible design — UV-transparent gel
trays, combs, and other accessories
are compatible with both new and
old models
Description
Visit us on the Web at explorer.bio-rad.com
DNA AND GENOMIC STUDIES: HORIZONTAL DNA ELECTROPHORESIS GEL BOXES
Mini-Sub® Cell GT Cells
105
DNA and Genomic Studies: DNA Electrophoresis Reagent Packs • Precast Agarose Gels
DNA Electrophoresis Buffers
Our DNA electrophoresis reagent packs are available in small, medium, and large sizes to
cover all your agarose gel needs and allow you to pour your own gels with ease. (UView™
loading dye and stain comes only in the small electrophoresis reagent pack size.) Choose
from our new UView loading dye and ultrasensitive nontoxic fluorescent stain, sensitive
nontoxic Fast Blast™ DNA stain, or the ultimate in sensitivity ethidium bromide solution.
Convenient premixed buffers ensure reproducible results.
Careful selection of both equipment and reagents can affect the quality of your
electrophoresis results. Bio-Rad pioneered the production of reagents specifically
designed for electrophoresis. Save preparation time and ensure reproducible results.
Small Fast Blast
DNA Electrophoresis
Reagent Pack
Small Ethidium Bromide
DNA Electrophoresis
Reagent Pack
Agarose Gel Drying Film and Agarose Powders
Premixed DNA Electrophoresis Sample Loading Buffer
Bio-Rad’s concentrated premixed DNA sample loading buffer contains two electrophoresis
tracking dyes (xylene cyanole FF and bromophenol blue) and glycerol in Tris buffer. Add
directly to liquid DNA samples and load your gels with ease.
Gel support films are a convenient, low-cost way to document and preserve agarose gels.
Simply lay a wet, stained agarose gel on a piece of film and let the moisture evaporate
overnight. The result is a durable dried gel.
Small UView DNA
Electrophoresis Reagent Pack
Certified™ Molecular Biology Agarose
This special agarose formulation provides exceptional DNA separation and more durable
gels, providing the sharpest resolution of fragments of 20 – 20,000 base pairs. The gels are
easy to handle and are recommended for low-percentage gels (0.8–3.0%).
Certified PCR Low-Melt Agarose
This agarose has a high sieving capacity and yields excellent resolution of fragments
≤1,000 bp in a low-melt or preparative format, ideal for digestion by agarose and for
all in-gel applications.
Gel Support Film
Catalog #
List Price
EDU Price
Small UView DNA Electrophoresis Reagent Pack
Makes 48 1% or 16 3% 7 x 10 cm agarose gels; includes 25 g agarose powder,
1 ml UView 6x loading dye and stain, 100 ml 50x TAE electrophoresis buffer
166-0462EDU
$145.00
$75.00
Small Fast Blast DNA Electrophoresis Reagent Pack
Makes 48 1% or 16 3% 7 x 10 cm agarose gels; includes 25 g agarose powder,
100 ml 500x Fast Blast DNA stain, 100 ml 50x TAE electrophoresis buffer
166-0450EDU
Medium Fast Blast DNA Electrophoresis Reagent Pack
Makes 270 1% or 90 3% 7 x 10 cm agarose gels; includes 125 g agarose
powder, 100 ml 500x Fast Blast DNA stain, 1 L 50x TAE electrophoresis buffer
166-0455EDU
270.00
189.00
Large Fast Blast DNA Electrophoresis Reagent Pack
Makes 1,080 1% or 360 3% 7 x 10 cm agarose gels; includes 500 g agarose
powder, 2 x 100 ml 500x Fast Blast DNA stain, 5 L 50x TAE electrophoresis buffer
166-0460EDU
700.00
560.00
Small Ethidium Bromide DNA Electrophoresis Reagent Pack
Makes 48 1% or 16 3% 7 x 10 cm agarose gels; includes 25 g agarose powder,
10 ml ethidium bromide, 200 ml 50x TAE electrophoresis buffer
166-0451EDU
Medium Ethidium Bromide DNA Electrophoresis Reagent Pack
Makes 270 1% or 90 3% 7 x 10 cm agarose gels; includes 125 g agarose
powder, 10 ml ethidium bromide, 1 L 50x TAE electrophoresis buffer
166-0456EDU
Large Ethidium Bromide DNA Electrophoresis Reagent Pack
Makes 1,080 1% or 360 3% 7 x 10 cm agarose gels; includes 500 g agarose
powder, 10 ml ethidium bromide, 5 L 50x TAE electrophoresis buffer
Agarose Gel Drying Film
Gel Support Film, for drying agarose gels, 65 x 125 mm, 50 sheets
Agarose Powders
Certified Molecular Biology Agarose, 5 g
Certified Molecular Biology Agarose, 25 g
Certified Molecular Biology Agarose, 125 g
Certified Molecular Biology Agarose, 500 g
Certified PCR Low-Melt Agarose, 25 g
Certified PCR Low-Melt Agarose, 125 g
Certified PCR Low-Melt Agarose, 500 g
Certified Agarose
Powders
DNA and Genomic
Reagents are perfect for
use with:
• Forensic DNA fingerprinting
kit (#166-0007EDU)
105.00
130.00
Precast Agarose Gels
55.00
69.00
• Analysis of precut lambda
DNA kit (#166-0001EDU)
• Restriction digestion and
analysis of lambda DNA kit
(#166-0002EDU)
• Crime Scene Investigator
PCR Basics™
(#166-2600EDU)
Precast Agarose Gel Features
• UV-transparent tray with fluorescent lane numbers and ruler
• Tray locks into Bio-Rad electrophoresis cells of corresponding size
• Mini 7 x 10 cm and wide mini 15 x 10 cm formats
• Mini size fits most manufacturers’ mini gel boxes
• Made with TAE (TBE buffer options available online)
• Gel percentages for optimal resolution: 1.0%, 200 bp –10 kb or 3.0%, 20 bp –1 kb
• Shipment and storage at room temperature
• One year shelf life
166-0461EDU
170-2984EDU
161-3116EDU
161-3100EDU
161-3101EDU
161-3102EDU
161-3113EDU
161-3114EDU
161-3115EDU
667.50
169.00
17.50
59.00
169.00
543.00
138.00
404.00
1,532.00
182.00
534.00
118.30
14.00
47.20
135.20
434.00
110.40
323.20
1,225.60
DNA Electrophoresis Buffer
in multiple-class sized 5 L cubes
Precast Polyacrylamide Gels for DNA
Mini-PROTEAN® polyacrylamide precast gels are designed to fit the Mini-PROTEAN® Tetra
cell and are ready to run.
TBE Gels for Nucleic Acids
Mini-PROTEAN TBE gels provide high-resolution separation of nucleic acids from 50 to
1,750 base pairs (higher resolution than agarose gel) — ideal for analyzing the purity of
PCR fragments. See p. 123 for more details and ordering information.
ReadyAgarose
Precast Mini Gel
Ordering Information
Description
260.00
DNA Electrophoresis
Buffers in convenient
1 L bottles
Our convenient ReadyAgarose™ gels are individually packaged precast agarose gels.
Ordering Information
Description
Premixed DNA Electrophoresis Running Buffers
Just dilute and run. Our complete line of premixed buffers is made with Bio-Rad
electrophoresis-purity reagents.
Additional supplies:
Catalog #
List Price
EDU Price
EDU 10+
• GMO Investigator™ kit
(#166-2500EDU)
DNA Electrophoresis Buffers
50x TAE, 100 ml
50x TAE, 1 L
50x TAE, 5 L cube
10x TBE, 1 L
10x TBE, 5 L cube
Sample Loading Dye, 6x, 1 ml
Sample Loading Dye, 5x, 10 ml
166-0742EDU
161-0743EDU
161-0773EDU
161-0733EDU
161-0770EDU
166-0401EDU
161-0767EDU
$18.75
82.00
235.00
36.00
107.00
23.75
20.00
$15.00
65.60
188.00
28.80
85.60
19.00
16.00
—
—
—
—
—
—
—
• Comparative proteomics
kit I: protein profiler module
(#166-2700EDU)
Buffer Reagent Components
Tris, 500 g
Bromophenol Blue, 10 g
Xylene Cyanole FF, 25 g
161-0716EDU
161-0404EDU
161-0423EDU
32.00
38.00
107.00
25.60
30.40
85.60
—
—
—
• Cloning and Sequencing
Explorer Series
(#166-5000EDU)
ReadyAgarose Precast Mini Gels, TAE, 7 x 10 cm
1.0%, 2 x 8-well
161-3057EDU
1.0%, 8-well
161-3015EDU
3.0%, 8-well
161-3017EDU
11.00
11.00
12.00
8.80
8.80
9.60
8.25
8.25
9.00
• PV92 PCR informatics kit
(#166-2100EDU)
• Fish DNA Barcoding kit
(#166-5100EDU)
• DNA electrophoresis cells,
pp. 104– 105
• DNA size standards, p. 108
• DNA stains, p. 109
DNA AND GENOMIC STUDIES: DNA ELECTROPHORESIS REAGENT PACKS • PRECAST AGAROSE GELS
DNA Electrophoresis Reagent Packs
Quantity discount applies to ten or more items of the same catalog number.
Buffers, reagents, and ReadyAgarose gels ship and store at room temperature. For a complete listing of our varieties of
ReadyAgarose gels, including gels with ethidium bromide, and buffers visit us on the Web at discover.bio-rad.com.
106
Visit us on the Web at explorer.bio-rad.com
107
DNA and Genomic Studies: DNA Electrophoresis Size Standards • DNA Stains
DNA Stains
Bio-Rad’s high-quality DNA size standards are essential tools for every classroom
molecular biologist. DNA size standards can be used as positive controls for
electrophoresis and are used as references to determine the sizes of unknown
DNA fragments.
Fast Blast™ DNA Stain
Fast Blast DNA stain is an ultrasensitive, convenient, inexpensive, and nontoxic
alternative to ethidium bromide for the detection of DNA. This unique product stains DNA
deep blue in both agarose and polyacrylamide gels, providing vivid, consistent results.
EZ Load™ molecular rulers cover all your needs for sizing DNA fragments, from
oligonucleotides to PCR products to plasmids. Our PCR ruler is a 100 bp ladder
that extends to 3 kb — ideal for even the longest PCR products. To ensure easy
and correct measurement of the sizes of your samples, most of these ladders
include a visually distinct reference band.
Fast Blast DNA stain is packaged as 100 ml of a 500x concentrate that must be
diluted before use. Use Fast Blast to:
•
•
•
•
Additional supplies:
• ReadyAgarose™ gels and agarose powders, pp. 106–107
• DNA stains, p. 109
Fast Blast dye molecules are positively charged and when placed in an agarose gel will
migrate toward the negative electrode during electrophoresis, providing a striking and
inexpensive visual demonstration of the movement of molecules during electrophoresis.
New UView™ Loading Dye and Stain
UView 6x loading dye is a fluorescent nucleic acid stain and loading dye that enables
immediate visualization of your DNA postelectrophoresis without staining or destaining or
incorporating into the agarose prior to electrophoresis. It is nontoxic for easy disposal and
nonmutagenic so will not interfere with downstream applications. Its sensitivity is close to
that of ethidium bromide (>10 ng). This product is available in 200 µl or 1 ml volumes.
1,000 bp
1,000 bp
15 kb
900
800
700
3,000 bp
600
5,000 bp
500
4,000
7.0
1,000 bp/
100 ng
6.0
500 bp/
50 ng
2,000
300
200
A
700 bp/
70 ng
4.0
1,500
100 bp/
10 ng
2.0
500
C EZ Load 100 bp PCR
(100 – 3,000 bp)
200 bp/
20 ng
200
D EZ Load 500 bp
(500 – 8,000 bp)
1,000
E
EZ Load 1 kb
(1–15 kb)
F
EZ Load Precision
Molecular Mass Ruler
100
100
500
20
A
B
C
1.0
D
E
EZ Load 20 bp
(20 –1,000 bp)
B EZ Load 100 bp
(100 –1,000 bp)
3.0
1,000
DNA Electrophoresis Size Standards
EZ Load 20 bp Molecular Ruler
EZ Load 100 bp Molecular Ruler
EZ Load 100 bp PCR Molecular Ruler
EZ Load 500 bp Molecular Ruler
EZ Load 1 kb Molecular Ruler
EZ Load Precision Molecular Mass Ruler
UView Loading Dye Time Savings
Step
Other Stains
(postelectrophoresis stain), min
Other Stains
(in gel prep), min
Add stain to agarose gel prep
NA
5
Add loading dye to sample,
load sample, run gel
26
26
≥20
0
1
1
1
>47 min
>32 min
>27 min
Stain/destain
See results
Total time
UView 6x
Loading Dye, min
Time Savings
0
26
Time Savings
0
Gel with UView Loading Dye
F
Ethidium Bromide Solution
When sensitivity is a must, Bio-Rad’s premixed ethidium bromide solution eliminates
preparation steps and minimizes exposure to hazardous ethidium bromide.
Ordering Information
Description
DNA Stains
Molecular Ruler
5.0
3,000
400
Stain DNA in agarose gels after electrophoresis, in less than 15 minutes or overnight
Stain DNA during electrophoresis
Teach students basic principles of electrophoresis
Stain nuclei in intact cheek cells
Size range
20 –1,000 base pairs, 50 bands, 500 µl
100 –1,000 base pairs, 10 bands, 500 µl
100 – 3,000 base pairs, 30 bands, 500 µl
500 – 8,000 base pairs, 16 bands, 500 µl
1,000 –15,000 base pairs, 15 bands, 500 µl
100 –1,000 base pairs, 5 bands, 500 µl
Catalog #
170-8351EDU
170-8352EDU
170-8353EDU
170-8354EDU
170-8355EDU
170-8356EDU
List Price
$112.00
112.00
112.00
112.00
91.00
112.00
EDU Price
$89.60
89.60
89.60
89.60
72.80
89.60
DNA standards are shipped at room temperature. Store in the freezer upon receipt. Detailed protocols, applications, and ordering information for
all Bio-Rad DNA size standards are available online. Visit us on the Web at discover.bio-rad.com.
Silver Stain Plus™ Kit
Silver Stain Plus is our most sensitive and easiest to use silver stain — ideal for
staining both nucleic acids and proteins in polyacrylamide and agarose gels. Sensitivity
is better than 100 ng/band.
Additional supplies:
• DNA electrophoresis cells,
pp. 104– 105
DNA AND GENOMIC STUDIES: DNA ELECTROPHORESIS SIZE STANDARDS • DNA STAINS
DNA Electrophoresis Size Standards
• Gel support film for agarose,
p. 106
• ReadyAgarose™ gels and
Ordering Information
Description
agarose powders, pp. 106– 107
Catalog #
List Price
EDU Price
UView Loading Dye and Stain
UView 6x loading dye, 0.2 ml
UView 6x loading dye, 1 ml
166-5111EDU
166-5112EDU
$31.25
95.00
$25.00
76.00
DNA Stains
Fast Blast DNA Stain, 500x, 100 ml
Ethidium Bromide Solution, 10 mg/ml, 10 ml
Silver Stain Plus Kit
166-0420EDU
161-0433EDU
161-0449EDU
30.00
39.00
205.00
24.00
31.20
164.00
• Mini-PROTEAN® Tetra cell,
p. 120
• Mini-PROTEAN TBE
polyacrylamide gels for highresolution DNA separation,
p. 123
Detailed protocols for Fast Blast DNA stain are available at explorer.bio-rad.com.
108
Visit us on the Web at explorer.bio-rad.com
109
DNA and Genomic Studies: PCR Thermal Cyclers
PCR Thermal Cycler Selection Guide
Bio-Rad offers a thermal cycler to meet your needs. Choose the sample capacity, upgrade options,
and features that are right for you. For more information, go to www.bio-rad.com/cyclers.
T100™ Thermal Cycler
With its 96-well sample capacity and rapid heating and cooling technology, this compact thermal
cycler provides high sample throughput in less time. This classroom-scale instrument also features
a large 5.7 inch touch screen, intuitive programming, and real-time graphical display of your PCR
protocol at an economical price.
96 wells x 0.2 ml
Up to 4°C/sec
4 –100°C
1–100 µl
Included
5.7 inch VGA color touch screen
1 USB A
500 typical programs onboard; unlimited with USB flash drive expansion
26 x 47 x 23 cm (10 x 18 x 9 inches) (W x D x H)
T100 Thermal Cycler
C1000 Touch Thermal Cycler — Fast, Friendly, and Flexible
The C1000 Touch thermal cycler maximizes what a thermal cycler can do for your teaching laboratory.
This state-of-the-art instrument accommodates multiple sample formats, has an easy-to-use graphical
interface and, for truly sophisticated applications, can be upgraded for real-time quantitative PCR. For
more information on the modular C1000 Touch thermal cycler system, including the interchangeable
sample blocks and the real-time upgrade, visit us at www.bio-rad.com/cyclers.
™
• Precision temperature control
• Fast and easy protocol programming — including the protocol autowriter that automatically
generates protocols
• Interchangeable block options include 2 x 48 x 0.2 ml sample block and 96 x 0.2 ml sample block
• Gradient design for maximum reagent optimization
• Able to save protocols to USB flash drive
Specifications
Sample capacity
Speed of ramping
Temperature range
Heated lid
Reaction volumes
Gradient capability
Display
Ports
Memory
Dimensions
2 x 48 x 0.2 ml or 96 wells x 0.2 ml
Up to 5°C/sec with 96-well fast reaction module
0–100°C
Adjustable to 110°C
15–100 µl
Included
12 x 9 cm, high-resolution, color
5 USB A, 1 USB B
>1,000 typical programs onboard, unlimited with USB flash drive expansion
33 x 46 x 20 cm (13 x 18 x 8") (W x D x H)
Thermal cycler
T100
C1000 Touch
Peltier-effect technology
Yes
Yes
—
Yes
Upgrade to real-time PCR
Specifications
Sample capacity
Speed of ramping
Temperature range
Reaction volumes
Gradient capability
Display
Ports
Memory
Dimensions
DNA AND GENOMIC STUDIES: PCR THERMAL CYCLERS
PCR Thermal Cyclers
Interchangeable reaction modules
—
Yes
Number of wells
96
Dual 48/48 or 96
Gradient capability
Yes
Yes
Touch screen
Yes
Yes
Graphical user interface
Yes
Yes
Fast PCR protocol templates
Yes
Yes
Choice of temperature control
Yes
Yes
Power failure restore
Yes
Yes
Programmable ramp rates
Yes
Yes
Reports on cycler use and preferences
Yes
Yes
Heated lid
Yes
Yes
Protocol autowriter
USB flash drive compatibility
Networking capability
Energy efficient
C1000 Touch Thermal Cycler
—
Yes
Yes
Yes
—
Yes
Yes
Yes
PCR Plastic Supplies
Thin-Wall Microcentrifuge Tubes and Strips for PCR Applications
Bio-Rad’s PCR tubes and strips are specially designed and engineered to maximize DNA amplification.
A unique manufacturing process provides consistent, uniformly thin walls and bubble tops (200 µl
sizes), allowing optimal heat transfer. To access our selection charts for help choosing the correct
PCR tube based on your thermal cycler, reaction volume, and other application requirements, please
visit us on the Web at discover.bio-rad.com or request bulletins 5258 and 6090.
Thin-Wall PCR Tubes
Easy Cap™ Tool and PCR Strip Cap Tool
The multifunctional Easy Cap tool facilitates cap opening and closing. It prevents crushing of 0.5 ml
and 0.2 ml thin-wall PCR tubes, and it properly seats domed caps on tubes or PCR plates. The PCR
Strip Cap tool facilitates PCR strip cap closing. Ensures proper seating of strip caps on PCR plates.
Ordering Information
Ordering Information
Description
Description
Catalog #
List Price
EDU Price
Thermal Cyclers
T100 Thermal Cycler, 120/240 V
186-1096EDU
$4,495.00
$3,371.25
C1000 Touch Thermal Cycler with Dual 48/48 Fast Reaction
Module, includes thermal cycler chassis, dual 48/48 fast
reaction module, USB flash drive
185-1148EDU
8,195.00
6,556.00
C1000 Touch Thermal Cycler with 96-Well Fast Reaction
Module, includes thermal cycler chassis, 96-well fast
reaction module, USB flash drive
185-1196EDU
7,495.00
5,996.00
C1000 Touch 48-Well Reaction Block
184-0148EDU
2,995.00
2,396.00
C1000 Touch 96-Well Reaction Block
184-0196EDU
2,695.00
2,156.00
InstaGene™ matrix, p. 116
Xcluda™ pipet tips, p. 140
110
PCR Plastics (T100™, and C1000 Touch™ thermal cyclers)
0.2 ml Tubes with Flat Caps, clear, 1,000
0.2 ml Tubes with Domed Caps, clear, 1,000
Domed 8-Cap Strips, for 0.2 ml tubes and plates, clear, 120
0.2 ml 8-Tube Strips Without Caps, clear, 125
Domed 12-Cap Strips, for 0.2 ml tubes and plates, clear, 200
0.2 ml 12-Tube Strips without Caps, clear, 100
Multiplate™ PCR Plastics (for C1000 Touch thermal cyclers)
Multiplate Low-Profile 48-well Unskirted PCR Plates, white, 50
Multiplate 96-well Unskirted PCR Plates, clear, 25
(also for T100 Thermal Cycler)
PCR Accessories
PCR Tube Capless Adaptors, 500
Easy Cap Tool
PCR Strip Cap Tool
Catalog #
List Price
EDU Price
TFI-0201EDU
TWI-0201EDU
TCS-0801EDU
TBS-0201EDU
TCS-1201EDU
TBS-1201EDU
$56.00
56.00
36.00
94.00
67.00
117.00
$44.80
44.80
28.80
75.20
53.60
93.60
MLL-4851EDU
MLP-9601EDU
128.00
98.00
102.40
78.40
223-9500EDU
ECT-1000EDU
ECT-2000EDU
24.00
16.00
39.00
19.20
12.80
31.20
Thin-Wall PCR Tube and Cap Strips
Easy Cap Tool
PCR Strip Cap Tool
Visit us on the Web at explorer.bio-rad.com
111
DNA and Genomic Studies: Real-Time PCR Detection Systems
Real-Time PCR Detection Systems
Bio-Rad’s real-time PCR detection systems are modular systems that add real-time PCR
capabilities to a base thermal cycler. The detection systems are optical modules that allow
target sequence detection via fluorescent probes or DNA binding dyes such as SYBR® Green.
Sample quantitation by real-time PCR increases reliability of gene expression analysis,
improves viral load assessment, and accelerates throughput in screening applications.
Feature
Excitation range
CFX Connect™ Real-Time PCR Detection System
The CFX Connect real-time PCR detection system is an affordable and dependable
two-target qPCR instrument that incorporates industry leading performance with the
most user friendly software on the market. The system features include:
• Easy startup — obtain great results right away with factory-calibrated dyes, quick setup,
and intuitive software
• Effortless optimization — save time and reduce costs by optimizing assays in a single
run using the thermal gradient
• Powerful data analysis tools — quickly and accurately validate and analyze data with
the advanced analysis modules of CFX Manager™ software
•
•
•
•
Set up your system quickly — easy installation and factory-calibrated optics
Minimize sample and reagent use — up to five-target multiplexing (volumes as low as 10 µl)
Optimize reactions in a single run — thermal gradient feature
Analyze data faster — visualize all run data at once and export only the data you need
in the format you want
Advanced data analysis tools — normalized gene expression using CFX Manager software
Configure the system to fit your needs — run without a computer, run up to four systems
from one computer, or integrate with the CFX automation system for higher throughput
Streamline publication submission — include MIQE annotations and generate RDML files
using Biogazelle’s qbasePLUS software
8.5 inch VGA color touch screen
450–684 nm
3 LEDs in
optics shuttle
6 LEDs in
optics shuttle
Optical detection
3 photodiodes
6 photodiodes
Gradient range
Maximum gradient span
Maximum ramp rate
Dimensions (W x D x H)
Weight
Base thermal cycler
Software
Integrated gene expression analysis
Touch screen
HRM compatibility
The CFX96 Touch system’s solid-state optical technology (six filtered LEDs and six filtered
photodiodes) maximizes fluorescence detection for specific dyes in specific channels,
providing sensitive detection for precise quantitation and target discrimination. The
system features include:
•
•
•
•
450 –535 nm
Multiplex capability
CFX96 Touch™ Real-Time PCR Detection System
The CFX96 Touch optical reaction module converts a C1000™ thermal cycler into a
powerful and precise real-time PCR detection system. This six-channel real-time system
includes advanced optical technology combined with precise thermal control to deliver
sensitive, reliable detection.
CFX96 Touch
Light source
Detection range
CFX Connect Real-Time PCR
Detection System
CFX Connect
515 – 580 nm
515 – 730 nm
Up to 2 targets
Up to 5 targets
30 –100°C
30 –100°C
24°C
24°C
5°C/sec
5°C/sec
33 x 46 x 36 cm
33 x 46 x 36 cm
21 kg
21.4 kg
CFX Connect
C1000 Touch™
CFX Manager and
qbasePLUS software
CFX Manager and
qbasePLUS software
Yes
Yes
—
Yes
Yes
Yes
Ordering Information
Description
Catalog #
List Price
EDU Price
185-5200EDU
$24,950.00
$19,960.00
CFX Connect Real-Time PCR Detection System includes CFX Connect thermal cycler
chassis, CFX Connect optical reaction module, CFX Manager software, qbasePLUS
software license, cables. Computer not included.
185-5201EDU
24,950.00
19,960.00
Laptop PC Computer with Intel Core
170-7369EDU
1,901.00
1,520.80
185-5196EDU
35,050.00
28,040.00
184-5096EDU
29,950.00
23,960.00
TLS-0851EDU
TCS-0803EDU
101.00
36.00
80.80
28.80
MLL-4851EDU
MLL-9651EDU
128.00
112.00
102.40
89.60
CFX Connect Real-Time PCR Detection System
CFX Connect Real-Time PCR Detection System includes CFX Connect thermal cycler
chassis, CFX Connect optical reaction module, CFX Manager software, qbasePLUS
software license, cables, reagents, consumables. Computer not included.
CFX96 Touch Real-Time PCR
Detection System
CFX96 Touch Real-Time PCR Detection System and Components
CFX96 Touch Real-Time PCR Detection System includes C1000 Touch thermal cycler
chassis, CFX96 optical reaction module, CFX Manager software, qbasePLUS
software license, cables, reagent and consumable samples
CFX96 Optical Reaction Module includes CFX Manager software, qbasePLUS software
license, communication cables, reagent and consumable samples, for use with
C1000 Touch thermal cycler chassis
PCR Plastics (for CFX Systems)
Low-Profile 0.2 ml 8-Tube Strips without Caps, white, 120 (CFX96 real-time PCR systems)
Optical Flat 8-Cap Strips, for 0.2 ml tubes and plates, ultraclear, 120
(CFX Connect and CFX96 systems)
Multiplate™ PCR Plastics (for CFX Systems)
Multiplate Low-Profile 48-Well Unskirted PCR Plates, white, 50
Multiplate Low-Profile 96-Well Unskirted PCR Plates, white, 25
DNA AND GENOMIC STUDIES: REAL-TIME PCR DETECTION SYSTEMS
Real-Time PCR Detection Systems
For real-time PCR kits please refer to pp. 64–65
For PCR reagents please refer to p. 115
112
Visit us on the Web at explorer.bio-rad.com
113
DNA and Genomic Studies: Amplification/PCR Reagents • Gene Transfer Technology • Cell
Bio-Rad’s reagents are specially formulated to meet both conventional and real-time
application requirements with minimal need for optimization. All reagents are qualified for
use on Bio-Rad’s complete line of thermal cyclers and real-time PCR detection systems and
demonstrate high performance for cDNA, genomic DNA, and plasmid DNA over a wide
dynamic range. For more information, visit us at www.bio-rad.com/pcrreagents.
2x Master Mix
2x Master Mix for PCR
The 2x master mix for PCR is a concentrated solution of Taq DNA polymerase, dNTPs, and all
the components required for PCR (except DNA template and primers). This premixed formulation
saves time and reduces contamination thanks to a reduced number of pipetting steps required
for PCR setup. The absence of endodeoxyribonucleases, exodeoxyribonucleases, and
ribonucleases is confirmed by appropriate quality control tests. The master mix is functionally
tested in amplification of a single-copy gene from human genomic DNA.
iTaq™ DNA Polymerase and dNTP Mix
iTaq DNA polymerase is an antibody-mediated hot-start DNA polymerase that is suitable
for both conventional and real-time PCR applications and that ensures ease of use, high
specificity, and sensitivity.
iTaq DNA Polymerase
iQ SYBR® Green Supermix
114
List Price
EDU Price
2x Master Mix for PCR, 90 units, 1.2 ml, includes 0.075 units per µl Taq DNA polymerase (recombinant),
reaction buffer, 4 mM MgCl2, 0.4 mM of each dNTP (dATP, dCTP, dGTP, dTTP)
166-5009EDU
$92.50
$74.00
iTaq DNA Polymerase, 5 U/µl, includes 250 U polymerase, 1.25 ml of 10x PCR buffer, 1.25 ml of 50 mM
MgCl2 solution
170-8870EDU
225.00
180.00
dNTP Mix, 200 µl premixed solution, contains 10 mM each dNTP (dATP, dCTP, dGTP, dTTP)
170-8874EDU
120.00
96.00
MgCl2 solution, 50 mM, 1.25 ml
170-8872EDU
26.00
20.80
iQ™ SYBR® Green Supermix, 100 x 50 µl reactions, 2x mix contains 100 mM KCl, 40 mM Tris/HCl, pH 8.4,
0.4 mM each dNTP (dATP, dCTP, dGTP, dTTP), 50 U/ml iTaq DNA polymerase, 6 mM MgCl2, SYBR® Green I,
20 nM fluorescein, stabilizers
170-8880EDU
183.00
146.40
iScript™ One-Step RT-PCR Kit with SYBR® Green, 50 x 50 µl reactions, includes iScript reverse transcriptase
for one-step RT-PCR, 2x SYBR® Green RT-PCR reaction mix, nuclease-free water
170-8892EDU
189.00
151.20
CHO-1411EDU
51.00
40.80
165-2100EDU
2,085.00
1,668.00
165-2081EDU
165-2082EDU
165-2083EDU
15.00
15.00
15.00
12.00
12.00
12.00
170-3342EDU
204.00
163.20
145-0103EDU
4,612.00
3,689.60
TC20 Automated Cell Counter 100–240 V, includes instrument, USB flash drive and cable, 30 TC20 dual-chamber
counting slides (60 counts), 1.5 ml TC20 trypan blue dye
145-0102EDU
4,094.00
3,275.20
TC20 Counting Slides, 5 x 30 slide pack of dual-chamber slides (300 counts), each slide can provide counts for
2 separate samples or dilutions
145-0015EDU
246.00
196.80
TC20 Trypan Blue Dye, 5 x 1.5 ml, sufficient for 750 counts (10 µl/count), 0.4% in 0.81% sodium chloride and
0.06% potassium phosphate dibasic solution, sterile filtered
145-0021EDU
42.00
33.60
TC20 Verification Kit, kit for validation of TC20 automated cell counter functionality, includes TC20 verification
slide, protocol
145-0014EDU
871.00
696.80
Chill-out™ Liquid Wax, clear, optical grade, 100 ml
MicroPulser Electroporator
• Simple one-button pulse delivery, attached cuvette chamber, and rapid charge time
• Preset optimized program selections for commonly studied bacteria and fungi
• Broad range of parameters for manual program optimization: voltage range of 200 – 3,000 V
with 10 V precision, and pulse range of 1.0 – 4.0 ms with 0.1 ms precision
• 3,000 V capability yields improved efficiency in larger-volume cuvettes
• Audible and visual pulse indicators
Chemi-Competent Cells
For applications ranging from the creation of cDNA libraries to the investigation of gene
function, competent cells have become an essential tool in research. Bio-Rad’s C-Max™
chemi-competent cells provide high-efficiency convenience for most routine cloning
experiments.
Catalog #
Amplification/PCR Reagents
Introducing DNA and RNA into cells is a powerful tool for evaluating gene expression. Bio-Rad’s
gene transfer products offer choices of products for gene transfer to bacterial, plant, fungal, and
animal cells. For more information, visit us at www.bio-rad.com/genetransfer.
Electroporation Cuvettes
High-quality electroporation cuvettes are essential for consistent pulse delivery and reproducible
results with your valuable samples. The precision pulse output of the MicroPulser™ is matched by
high-quality electroporation cuvette construction. Bio-Rad’s cuvettes are thoroughly washed to
eliminate trace inhibitors, then sterilized and individually wrapped.
TC20 Cell Counter with Printer
Ordering Information
Description
Gene Transfer Technology with Shocking Efficiency!
MicroPulser™ Electroporator for Microorganisms
The MicroPulser electroporator is a simple and versatile electroporator that enables safe
and reproducible transformation of bacteria, yeast, and other microorganisms. Key
features include:
• Count cells quickly, accurately, and consistently within 30 sec using the
built-in auto-focus
• Conserve precious cells — use only 10 µl of suspended cells
• Print count results and dilution calculations from the TC20 thermal label printer
• Transfer counts and cell images using a USB key and access up to 100 counts
stored onboard
• Trust your counts — confirm instrument functionality with the TC20 verification slide
Use the TC20 cell counter to determine total cell count without dye or use trypan blue dye to
assess total cell count and cell viability. Simply load sample, insert slide, and view results!
Bio-Rad’s dNTP mix is formulated for optimal performance in real-time PCR applications
and is also qualified for use in conventional PCR applications, providing consistent,
high-yield amplification.
SYBR® Green
iQ™ SYBR® Green supermix is formulated for optimal results in real-time PCR based on SYBR®
Green I detection. It contains SYBR® Green I dye, hot-start iTaq DNA polymerase, optimized buffer,
and dNTPs qualified for quantitative PCR. Alternately, Bio-Rad’s iScript™ one-step RT-PCR kit with
SYBR® Green allows cDNA synthesis and PCR to be conveniently carried out in the same tube.
TC20™ Cell Counter
The TC20 cell counter is an automated device that provides a total count of
mammalian cells and a live/dead ratio in one simple step with accurate, reproducible
results. Count cells prior to cell culture or before starting processes and analytical
techniques that require an accurate and consistent number of input cells. The TC20
automated cell counter helps you avoid the tedious task of counting cells with a
microscope and hemocytometer, which produces varying results. The TC20 automated
cell counter uses multifocal plane analysis to assess cell viability. And it demonstrates
accurate counts of viable cells across a range of cell concentrations and cell sizes.
Speed up your cell counting and accelerate your research. Start now and test drive
the cell counter via the TC20 interactive demo by visiting discover.bio-rad.com.
MicroPulser and Accessories
MicroPulser Electroporator, 120/220 V, includes chamber with leads, 10 sterile cuvettes
(5 each of 0.1 cm and 0.2 cm electrode gap)
Gene Pulser®/MicroPulser Cuvettes, 0.4 cm, 5/pk
Gene Pulser/MicroPulser Cuvettes, 0.2 cm, 5/pk
Gene Pulser/MicroPulser Cuvettes, 0.1 cm, 5/pk
Chemi-Competent Cells
C-Max™5α Chemi-Competent Cells, 10 x 0.05 ml
TC20 Cell Counter
TC20 Automated Cell Counter with Printer 100–240 V, includes instrument, USB flash drive and cable, TC20 thermal
label printer, 1 roll of 185 labels, 30 TC20 dual-chamber counting slides (60 counts), 1.5 ml TC20 trypan blue dye
Electroporation Cuvettes
Chemi-Competent Cells
For more information on the MicroPulser system, please visit us on the Web at discover.bio-rad.com.
InstaGene™ matrix, p. 116
Xcluda™ pipet tips, p. 140
Visit us on the Web at explorer.bio-rad.com
DNA AND GENOMIC STUDIES: AMPLIFICATION/PCR REAGENTS • GENE TRANSFER TECHNOLOGY • CELL COUNTING
Amplification/PCR Reagents
Counting
115
DNA and Genomic Studies: Nucleic Acid Purification Kits • DNA Model
New DNA Model
Bio-Rad offers a complete line of products for the isolation and purification of nucleic acids. From the
purification of milligram quantities of plasmid DNA for restriction analysis or transformation to the rapid
and efficient purification of PCR products, Bio-Rad provides you with high-quality kits that eliminate
time-consuming, labor-intensive steps.
As common as DNA is, it can be a tough topic to understand. But that is where
this colorful and attractive DNA model comes into play!
InstaGene Matrix
Crude Genomic DNA for PCR: InstaGene™ Matrix
InstaGene matrix, composed of specially formulated Chelex® resin, makes DNA sample preparation
fast, easy, and cost-effective, providing PCR-quality template DNA from crude genomic preparations
in less than 1 hour. The InstaGene matrix adsorbs cell lysis products and removes inhibitors such
as magnesium ions that interfere with the PCR amplification process. Simply place cells in a
microcentrifuge tube, add the InstaGene matrix, boil, and spin. PCR-ready DNA is in the supernatant
and can be transferred directly into PCR tubes.
Total RNA Isolation: Aurum™ Total RNA Mini Kit
The Aurum total RNA mini kit produces DNA-free total RNA from a wide range of starting
materials including cultured cells, bacteria, and yeast, as well as animal and plant tissues. The
Aurum total RNA mini kit can also be used for RNA cleanup and desalting.
Aurum Plasmid Mini Purification Kit
Our easy-to-use Aurum plasmid mini kit improves the efficiency and throughput of plasmid
purifications with a simple bind-wash-elute protocol using silica membranes. Lysed bacterial cultures
can be cleared either by centrifugation or by vacuum filtration with the Aurum vacuum manifold. The
clarified lysates are applied to plasmid binding columns and plates where DNA is bound, then washed,
and finally eluted — all in less than 10 minutes. The purified plasmid DNA can be immediately used in
any downstream molecular biology application. For more information, request bulletin 2664.
• All the pieces to build a biologically correct DNA model
• Over 2 feet tall
• A great way to incorporate STEM concepts through the use of modeling
Engage your students immediately with this friendly, interactive, foam model of
DNA. It’s easy — just attach and assemble the DNA strands and then twist
to transform it into the familiar double helix. Just like a puzzle — and just
as in nature — this DNA model fits together only one way: adenine (A)
pairs with thymine (T) and guanine (G) pairs with cytosine (C) on the
sugar-phosphate backbones. Build a biologically correct DNA model
that can be used in a number of fun and educational ways!
Aurum Total RNA Mini Kit
Quantum Prep® Plasmid Miniprep Kit
Obtain optimal yield of plasmids for gene transfer, restriction, ligation, and cloning protocols. The
Quantum Prep miniprep kit is tested to ensure at least 20 µg of high-copy plasmid yield from just
1.5 ml of bacterial culture. The Quantum Prep miniprep procedure takes less than 15 minutes from
cell culture to purified plasmid, using an easy spin column–based procedure. DNA is recovered in
water or Tris/EDTA buffer for immediate use in mapping, sequencing, transfection, transformation,
PCR, or subcloning.
PCR Kleen™ Spin Purification Columns
Try our prepacked spin columns for purifying PCR products and other DNA molecules >200 bp
directly from reaction mixtures. A simple 4 minute spin effectively removes salts, nucleotides,
enzymes, primers, and primer-dimers. Purified DNA fragments are eluted into a collection tube and
are immediately available for secondary PCR, subcloning, restriction digests, ligations, sequencing,
and other enzymatic manipulations.
Quantum Prep Plasmid Miniprep Kit
Learning opportunities for all levels of instruction
Constructing DNA models is a great way to learn about DNA structure,
function, and replication. Students at any grade level, K–12 and even
up to college level, can explore the structure of color-coded DNA
bases showing purines and pyrimidines which connect
to a continuous sugar-phosphate backbone.
This model is designed for any classroom
environment, from lessons on DNA structure
and function to an introduction to the
exciting world of DNA, this model will
easily introduce or reinforce any and
all DNA lab activities.
PCR Kleen Spin Purification Columns
Ordering Information
Description
Ordering Information
Description
InstaGene Matrix, 20 ml
DNA Model
DNA Model Small Classroom Set – pack of 4 DNA models
DNA Model Classroom Set – pack of 8 DNA models
Catalog # List Price EDU Price
732-6030EDU
$52.00
$41.60
Aurum Total RNA Mini Kit, 50 preps, includes 50 RNA binding columns, 50 capless
732-6820EDU
collection tubes, 100 capped sample tubes (2.0 ml), 50 capped sample tubes
(1.5 ml), 1 vial lyophilized DNase I, RNase-free reagents, protocol overview instructions
210.00
168.00
Quantum Prep Plasmid Miniprep Kit, 100 preps, includes 20 ml cell resuspension
solution, 25 ml cell lysis solution, 25 ml neutralization solution, 20 ml
Quantum Prep matrix, 63 ml wash solution, 100 spin columns, instructions
732-6100EDU
183.00
146.40
PCR Kleen™ Spin Purification Columns, 25
732-6300EDU
78.75
63.00
Aurum Plasmid Mini Purification Kit, 100 preps, includes plasmid-binding mini
columns, 100 capless collection tubes, reagents, protocol overview, instructions
732-6400EDU
217.14
152.00
Aurum Vacuum Manifold, includes column adaptor plate, 4 replacement luer caps,
A stage and B stage, waste collection tray, vacuum regulator and gauge, tubing,
protocol overview, instructions
732-6470EDU
691.00
552.80
DNA AND GENOMIC STUDIES: NUCLEIC ACID PURIFICATION KITS • DNA MODEL
Genomic DNA, RNA, and Plasmid Purification Kits
Catalog #
List Price
EDU Price
166-7015EDU
166-7016EDU
166-7017EDU
$31.24
112.50
218.75
$24.99
90.00
175.00
Aurum Plasmid Mini Purification Kit
Aurum Vacuum Manifold
Detailed protocols, applications, and ordering information for all Bio-Rad nucleic acid purification products are available online.
116
Visit us on the Web at explorer.bio-rad.com
117
Proteins and Proteomic Studies: Equipment and Reagents
Section Contents
Mini-PROTEAN® Tetra Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Midi Format Criterion™ Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
GelAir™ Gel Drying System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Western Blotting Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Precast Polyacrylamide Gels
Mini-PROTEAN® TGX™and TGX Stain-Free™ Precast Gels . . . . . . . . . . . . 123
Criterion™ Precast Gels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
Electrophoresis Buffers
Premixed Buffers for Electrophoresis . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Blotting Membranes and Reagents
Blotting Membranes for Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Protein Blotting Buffer Reagents and Detection Kits . . . . . . . . . . . . . . . . 125
Protein Electrophoresis Standards and Stains
Protein Electrophoresis Size Standards . . . . . . . . . . . . . . . . . . . . . . . . . . 126
Protein Electrophoresis Reagent Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . 126
Protein Stains . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
“
We cannot underestimate
the value of real. The student
approach to doing and learning,
the internalized meaning of the
experience, the lasting impression,
the ability to carry the experience
further into education and careers…
We have found that
Bio-Rad kits are extremely well
designed for classroom use,
can be used as stand-alone
laboratory experiences, or can be
integrated into creative storylines
demonstrating multiple applications
of common biotechnology-based
techniques and provide that real
experience in the classroom.
Now compare that to pretend.
3>
1>
2>
PROTEINS AND PROTEOMIC STUDIES: EQUIPMENT AND REAGENTS
Vertical Protein Electrophoresis and Blotting Equipment
4>
”
Tammy Mandell, PhD
Assistant Director, Education and Training
University of Florida
Gainesville, Florida
118
1. Edward Teller Education Center, University of California, Davis, California;
2. Heritage Middle School, Deltona, Florida; 3. Bio-Rad Teacher Workshop, Cayey,
Puerto Rico; 4. AP Biology Workshop, Stanford, California.
119
Proteins and Proteomic Studies: Vertical Protein Electrophoresis Gel Boxes and Gel Drying
Systems
GelAir™ Gel Drying System
Move beyond DNA and explore the complete framework of molecular biology:
The GelAir drying system is perfect for preserving polyacrylamide and agarose gels. Gels
dried between two sheets of cellophane are crystal clear with a glossy finish. The GelAir
system is easy to use, maintenance free, and fast. The dried
gels are ideal for densitometry, photodocumentation,
overheads, or long-term storage in lab notebooks.
DNA ³ RNA ³ Protein ³ Trait
Protein electrophoresis is the most frequently cited research technique. Our mini vertical
polyacrylamide gel electrophoresis systems are state-of-the-art research tools and are
perfectly adaptable to science education. The Mini-PROTEAN® Tetra cell and Criterion™
cell provide rapid, high-resolution separation of complex protein mixtures for protein
fingerprinting, identification, and sample purity analysis.
Mini-PROTEAN Tetra Cell
Mini-PROTEAN Tetra Cell
®
The best got better. The Mini-PROTEAN Tetra cell is the next-generation mini cell for 1-D
vertical gel electrophoresis. The Mini-PROTEAN Tetra cell incorporates innovative, userfriendly features that make running protein or DNA on polyacrylamide gels in your teaching
lab easier than ever. This cell is durable, versatile, and easy to assemble. It can
run either precast or handcast polyacrylamide gels in 30 minutes.
• Runs one to four mini gels (7 cm height x 8.5 cm width)
• Exclusive sample loading guides direct pipet tips into sample wells — no more
missing or doubling-up samples in a lane
• Leak-free electrophoresis and gel casting
• Indestructible molded polycarbonate construction throughout
• Ability to run either precast (Mini-PROTEAN® TGX™, TGX Stain-Free™, or Ready Gel®
precast gels) or handcast gels. Please see p. 123 for a complete selection of precast
polyacrylamide gels
Fast and Easy Tetra Module Assembly
4
1
GelAir Drying System
5
2
Ordering Information
Description
Midi Format Criterion™ Cell
• Runs one or two midi-size gels
(8.7 cm height x 13.3 cm width)
• Integrated buffer chamber
• Uses Criterion and Criterion XT gels
Catalog #
List Price
EDU Price
165-8005EDU
$451.00
$360.80
Mini-PROTEAN Tetra Cell for Bio-Rad Precast Gels, 4-gel system includes electrode assembly, companion
running module, tank, lid with power cables, mini cell buffer dam
165-8004EDU
588.00
470.40
Mini-PROTEAN Tetra Cell, 10-well, 0.75 mm thickness; 2-gel system includes 5 combs, 5 sets of glass plates,
casting stand, 2 casting frames, sample loading guide, electrode assembly, tank, lid with power cables,
mini cell buffer dam
165-8002EDU
708.00
566.40
Mini-PROTEAN Tetra Cell, 10-well, 1.0 mm thickness; 2-gel system includes 5 combs, 5 sets of glass plates,
casting stand, 2 casting frames, sample loading guide, electrode assembly, tank, lid with power cables,
mini cell buffer dam
165-8003EDU
708.00
566.40
Mini-PROTEAN Tetra Cell, 10-well, 0.75 mm thickness; 4-gel system includes 5 combs, 5 sets of glass plates,
2 casting stands, 4 casting frames, sample loading guide, electrode assembly, companion running module,
tank, lid with power cables, mini cell buffer dam
165-8000EDU
897.00
717.60
Mini-PROTEAN Tetra Cell, 10-well, 1.0 mm thickness; 4-gel system includes 5 combs, 5 sets of glass plates,
2 casting stands, 4 casting frames, sample loading guide, electrode assembly, companion running module,
tank, lid with power cables, mini cell buffer dam
165-8001EDU
897.00
717.60
Mini-PROTEAN Tetra Electrode Assembly
Mini-PROTEAN Tetra Companion Running Module
Buffer Tank, replacement
Buffer Tank and Lid, replacement
Cell Lid With Power Cables
165-8037EDU
165-8038EDU
165-8039EDU
165-8040EDU
165-8041EDU
183.00
173.00
60.00
178.00
115.00
146.40
138.40
48.00
142.40
92.00
10-Well Gel Sample Loading Guide
15-Well Gel Sample Loading Guide
Buffer Dams, 2
Replacement Power Cables
Gel Cutter, 1
Gel Releaser, 5
165-3146EDU
165-3132EDU
165-3130EDU
165-2948EDU
170-3760EDU
165-3320EDU
27.00
27.00
16.00
71.00
21.00
30.00
21.60
21.60
12.80
56.80
16.80
24.00
Criterion Cell
Criterion Cell, includes electrophoresis buffer tank, lid with power cables, 3 sample loading guides
(12+2 well, 18-well, 26-well)
165-6001EDU
538.00
430.40
165-1771EDU
165-1777EDU
165-1776EDU
165-1775EDU
165-1779EDU
165-1780EDU
161-0752EDU
1,711.00
1,388.00
128.00
189.00
60.00
62.00
43.00
1,368.80
1,110.40
102.40
151.20
48.00
49.60
34.40
Mini-PROTEAN Tetra Cell and Accessories
Mini-PROTEAN Tetra Cell for Bio-Rad Precast Gels, 2-gel system includes electrode assembly, tank, lid with
power cables, mini cell buffer dam
3
Teaching applications include:
• Comparative proteomics kit I: protein profiler module, pp. 38– 39
• pGLO™ kit SDS-PAGE extension, pp. 22– 23
• Protein Expression and Purification Series, pp. 76– 81
The Criterion cell is designed to run up to two prepackaged midi-size gel cassettes
(8.7 cm height x 13.3 cm width) with 18 or 26 wells, allowing for increased throughput as
well as greater capacity and enhanced band resolution. The tank requires only 1 L of buffer
with its optimized size. The drop-in precast gel cassettes with numbered wells make for
quick and easy gel setup. Please reference bulletin 2710 for complete information on the
Criterion system.
The GelAir dryer, 27 x 43 x 30 cm (W x D x H), is a
heated chamber that works like a convection oven to
dry mini gels in 45 minutes. The complete GelAir drying
system includes the GelAir dryer, two drying frames
with 16 clamps, assembly table, cellophane, Bio-Rad’s
gel drying solution, and a manual with optimized
drying protocols.
Criterion Cell
and Criterion
Precast Gel
Criterion Electrophoresis Cell
Assembly Made Easy
1. Drop in cassettes.
2. Pour in buffer and
load samples.
3. Close the lid and
start your run.
GelAir Gel Drying System and Accessories (available only in the U.S.)
GelAir Drying System, 120 V
GelAir Dryer, 120 V, drying oven only
GelAir Assembly Table
GelAir Drying Frames, 2 sets plus 16 clamps
GelAir Cellophane, 50 sheets
GelAir Drying Frame Clamps, 8
Gel Drying Solution, 1 L
For more information on the Mini-PROTEAN Tetra and related modules and on polyacrylamide gel-casting reagents and accessories, request bulletin 5535 or visit us
on the Web at bio-rad.com/verticalelectro. For more technical information about the GelAir drying system, request bulletin 1965.
120
Visit us on the Web at explorer.bio-rad.com
PROTEINS AND PROTEOMIC STUDIES: VERTICAL PROTEIN ELECTROPHORESIS GEL BOXES AND GEL DRYING SYSTEMS
Vertical Gel Boxes
121
Proteins and Proteomic Studies: Western Blotting and Precast Polyacrylamide Gels
Mini-PROTEAN® TGX™ and TGX Stain-Free™ Precast Gels
Blotting expands the analytical possibilities for identification and characterization of proteins by immobilizing
proteins on synthetic membrane supports, followed by the use of various methods for detection of specific
molecules. Blotting offers an advantage over gel techniques by making proteins readily accessible to
antibodies following the transfer to the support.
The long shelf life Mini-PROTEAN TGX precast gels are part of an innovative gel
system designed to provide Laemmli-like separation patterns using a standard
Tris/glycine running buffer system and are compatible with Mini-PROTEAN
electrophoresis cells. Save 3 hours of class time by eliminating the need
to stain gels when using TGX Stain-Free precast gels. The gels include
unique tri-halo compounds that allow rapid fluorescent detection of
proteins with UV light. Benefits of all TGX gels include:
Mini Trans-Blot® System
Definitively identify proteins via western blotting. Following electrophoresis, use the Mini Trans-Blot
module to transfer proteins from a gel to a membrane. Visualize proteins of interest on the membrane
using one of Bio-Rad’s colorimetric detection assays. The Mini Trans-Blot module is interchangeable with
the Mini-PROTEAN® Tetra cell electrophoresis module, so the same tank and lid can be used.
Mini Trans-Blot System
• Blots up to two mini gels simultaneously
• High field strength for rapid 1 hour transfers
• Can be run overnight at low voltage
Get More Classroom Lab and Teaching Time
30 min, 200 V
Criterion™ Blotter
The Criterion blotter can be used to transfer either mini- or
midi-size gels, making it a flexible system for many lab setups.
• Transfer four mini gels or two midi gels
• Plate or wire electrode options
Trans-Blot Turbo Transfer System
®
™
1
Western blot of protein profiler gel
reveals precise location of myosin light chain
proteins (p. 40).
Trans-Blot Turbo system — provides innovation in protein transfer. The
Trans-Blot Turbo system reduces transfer protocols for gels to as little as 3 minutes while maintaining
high efficiency, high throughput, and the flexibility to run turbo or traditional semi-dry protocols. Please
reference bulletin 6039 for complete information on the Trans-Blot Turbo system.
Rapid transfer — transfers mini or midi gels in as little as 3 min
High throughput — can transfer 1–4 mini or 1–2 midi gels in a single run
Greater transfer efficiency — offers higher transfer efficiency compared to other transfer methods
Flexible design — allows user to customize transfer conditions and is compatible with traditional
semi-dry consumables
• Environmentally friendly — environmentally safe consumables eliminate disposal cost
Mini Trans-Blot Systems and Accessories
Mini-PROTEAN Tetra cell (2-gel) + Mini Trans-Blot module, includes 2 gel holder cassettes, 4 fiber pads, modular electrode
assembly, cooling unit, lower buffer tank, lid with cables
Mini-PROTEAN Tetra cell (2-gel) + Mini Trans-Blot module + PowerPac HC power supply, includes 2 gel holder cassettes,
4 fiber pads, modular electrode assembly, cooling unit, lower buffer tank, lid with cables
Mini Trans-Blot Module, lower buffer tank and lid not included
Gel holder cassette
Blue cooling Unit for Mini-PROTEAN Tetra tanks
Roller, 1
Criterion Blotting Systems
Criterion Blotter with plate electrodes, includes cell assembled with plate electrodes, lid with cables, 2 Criterion gel
holder cassettes, 1 pack precut blot absorbent filter paper, 4 fiber pads, gel/blot assembly tray, roller, sealed ice block
Criterion Blotter with wire electrodes, includes cell assembled with wire electrodes, lid with cables, 2 Criterion gel
holder cassettes, 1 pack precut blot absorbent filter paper, 4 fiber pads, gel/blot assembly tray, roller, sealed ice block
Trans-Blot Turbo Transfer System
Trans-Blot Turbo transfer system for rapid semi-dry transfer
Trans-Blot Turbo starter system, includes Trans-Blot Turbo system; PVDF and nitrocellulose mini (qty 3) and Midi (qty 2) transfer
packs; pack of Precision Plus Protein™ WesternC™ standards (250µl), and Precision Protein™ StrepTactin HRP conjugate (125 µl)
Trans-Blot Turbo Cassette, single
Trans-Blot Turbo Base, no cassettes
3
4
5
6
7
1
2
3
4
5
6
7
Protein Profiler Gels
(left, sample types by lane):
Precast Gels Are Available with
Different Combs
1. size standards
(250 kD, 150 kD, 100 kD,
75 kD, 50 kD, 37 kD, 25 kD,
20 kD, 15 kD, and 10 kD);
2. shark;
3. salmon;
4. trout;
5. catfish;
6. sturgeon;
7. actin and myosin standards.
4–20% TGX gel
Description
Trans-Blot Turbo Transfer System
List Price
EDU Price
Mini-PROTEAN TGX Precast Gels
7.5% Resolving Gel, 10 gels/box, 10-well, 30 µl
7.5% Resolving Gel, 2 gels/box, 10-well, 30 µl
7.5% Resolving Gel, 10 gels/box, 15-well, 15 µl
456-1023EDU
456-1023SEDU
456-1026EDU
$103.00
22.00
103.00
$82.40
17.60
82.40
10% Resolving Gel, 10 gels/box, 10-well, 30 µl
10% Resolving Gel, 2 gels/box, 10-well, 30 µl
10% Resolving Gel, 10 gels/box, 15-well, 15 µl
456-1033EDU
456-1033SEDU
456-1036EDU
103.00
22.00
103.00
82.40
17.60
82.40
12% Resolving Gel, 10 gels/box, 10-well, 30 µl
12% Resolving Gel, 2 gels/box, 10-well, 30 µl
12% Resolving Gel, 10 gels/box, 15-well, 15 µl
456-1043EDU
456-1043SEDU
456-1046EDU
103.00
22.00
103.00
82.40
17.60
82.40
List Price
EDU Price
166-0827EDU
$800.00
$600.00
166-0828EDU
1,733.33
1,300.00
4–15% Resolving Gel, 10 gels/box, 10-well, 30 µl
4–15% Resolving Gel, 2 gels/box, 10-well, 30 µl
4–15% Resolving Gel, 10 gels/box, 15-well, 15 µl
456-1083EDU
456-1083SEDU
456-1086EDU
103.00
22.00
103.00
82.40
17.60
82.40
170-3935EDU
170-3931EDU
170-3919EDU
165-1279EDU
456.00
92.00
31.00
37.00
364.80
73.60
24.80
29.60
4–20% Resolving Gel, 10 gels/box, 10-well, 30 µl
4–20% Resolving Gel, 2 gels/box, 10-well, 30 µl
4–20% Resolving Gel, 2 gels/box, 15-well, 15 µl
456-1093EDU
456-1093SEDU
456-1096EDU
103.00
22.00
103.00
82.40
17.60
82.40
Any kD™ Resolving Gel, 10 gels/box, 10-well, 30 µl
Any kD Resolving Gel, 2 gels/box, 10-well, 30 µl
456-9033EDU
456-9033SEDU
103.00
22.00
82.40
17.60
170-4070EDU
794.00
635.20
170-4071EDU
753.00
602.40
TGX Stain-Free™ Gels
4–20% Mini-PROTEAN TGX Stain-Free gels (10)
456-8093EDU
114.00
91.20
Mini-PROTEAN TBE Precast Gels
5% Resolving Gel, 2 gels/box, 10-well, 30 µl
10% Resolving Gel, 2 gels/box, 10-well, 30 µl
15% Resolving Gel, 2 gels/box, 10-well, 30 µl
4–20% Resolving Gel, 2 gels/box, 10-well, 30 µl
456-5013EDU
456-5033EDU
456-5053EDU
456-5093EDU
23.00
23.00
23.00
23.00
18.40
18.40
18.40
18.40
Criterion Tris-HCl Gels
15% Resolving gel, 18-well, 30 µl
4–20% Linear gradient, 18-well, 30 µl
345-0020EDU
345-0033EDU
15.00
15.00
12.00
12.00
2,830.00
2,995.00
2,264.00
2,396.00
170-4151EDU
170-4152EDU
702.00
1,575.00
561.60
1,260.00
Buffers and reagents ship at room temperature. Store as directed on label. For more western blotting and immunodetection reagents, visit us on the Web at discover.bio-rad.com.
For additional information on Criterion blotting system refer to bulletin 2558. The PowerPac HC power supply is recommended for Criterion blotting.
Outlined Sample Wells
After you remove the combs, the sample wells
are outlined on the outside of the precast gel
cassettes to simplify sample loading and
eliminate loading errors. Only from Bio-Rad!
Mini-PROTEAN TGX Innovations
Catalog #
Catalog #
170-4150EDU
170-4155EDU
• 10-well combs are most popular in teaching
labs and accommodate samples up to 30 µl
• 15-well combs are designed for samples
up to 20 µl
4–20% TGX gel
Ordering Information
Ordering Information
Description
2
20 min, 300 V
Criterion Blotter
PowerPac™ HC power supply
recommended
•
•
•
•
122
• Reduced run times — up to 30% faster
• Up to 12 months of shelf life at 4ºC
• Bottom-open cassette design for simple gel handling and blotting
PROTEINS AND PROTEOMIC STUDIES: WESTERN BLOTTING
Western Blotting Systems
Outlined
and
numbered
wells
Alignment
arrows for
opening
cassette
Criterion Tris-HCI precast gels ship on blue ice and should be stored at 4ºC.
Shelf life is 3 months from date of manufacture. Additional freight charge for blue ice (see p. 145).
Visit us on the Web at explorer.bio-rad.com
123
Proteins and Proteomic Studies: Precast Gels • Electrophoresis Buffers and Reagents
Protein Blotting Buffer Reagents and Detection Kits
Get the essential reagents to perform colorimetric detection of
proteins on western blots with the convenience of premixed buffers
and enzyme substrate kits. Select from a combination of binding
conjugates and color detection reagents, including alkaline
phosphatase (AP)- or horseradish peroxidase (HRP)-conjugated
secondary antibodies and HRP-conjugated protein A or protein G.
Visit us on the Web at discover.bio-rad.com for details about
which kit is right for your application.
Bio-Rad pioneered the production of reagents for electrophoresis.
This section describes our convenient premixed electrophoresis
buffers — the perfect solutions to your classroom electrophoresis
and blotting needs!
Save preparation time and ensure perfect electrophoresis results
every time. Just dilute and run using our premixed 10x buffer
concentrates. Our buffers are made with electrophoresis-purity
reagents and are quality controlled to ensure reproducible results.
Electrophoresis buffers are available in two formulations:
Detailed protocols, applications, and ordering information for all
Bio-Rad blotting equipment, detection kits, and reagents are
available online. Visit us on the Web.
• Tris/glycine/SDS buffer for standard vertical polyacrylamide gel
electrophoresis (SDS-PAGE) applications
• Tris/glycine buffer for native or nondenaturing PAGE
Premixed Sample Buffer for Protein Electrophoresis
Simplify sample preparation and save time! Bio-Rad’s Laemmli sample
loading buffer contains the electrophoresis tracking dye bromophenol
blue, SDS, glycerol, and Tris buffer. Add directly to samples to solubilize
proteins, boil, and then load your gels with ease.
Colorimetric Detection of Proteins
Specific enzymatic detection of membrane-bound antigens.
1. A membrane with unoccupied binding
sites is incubated with primary antigen.
2. Primary antibody to a specific antigen
is incubated with the membrane.
3. A blotting-grade antibody-enzyme
conjugate is added to bind to the
primary antibody.
4. Color development reagent is added to
the blot. The HRP or AP enzyme catalyzes
the conversion of the substrate (S) to a
colored precipitate (P) at the site of the
antigen-antibody complex.
S
4
P
3
2
1
Ordering Information
Description
Protein migration on TGX precast gels. Percentages refer to acrylamide
percentages. The molecular mass of each band is given in kD.
Blotting Membranes and Reagents
Blotting Membranes for Proteins
Bio-Rad offers a comprehensive line of blotting membranes available in rolls or
in sheets cut to fit the Mini Trans-Blot® module and Criterion™ blotter. Membranes
are also available in preassembled blotting membrane/filter paper sandwiches. For
a complete list of blotting membranes, please visit us at discover.bio-rad.com.
Nitrocellulose membrane (0.45 µm) is most commonly used as a transfer
medium for proteins. For transfer of low molecular weight proteins (<15,000),
0.2 µm nitrocellulose membrane provides better sensitivity, especially with
immunodetection assays.
Bio-Rad’s premixed
electrophoresis buffers
save time and ensure quality.
Electrophoresis and Blotting Buffers and Reagents
10x Tris/Glycine/SDS, 1 L
10x Tris/Glycine/SDS, 5 L cube
10x Tris/Glycine, 1 L
10x Tris/Glycine, 5 L cube
Laemmli Sample Buffer, 30 ml
Native PAGE Sample Buffer, 30 ml
Tris, 500 g
Urea, 250 g
Glycine, 250 g
Bromophenol Blue, 10 g
Dithiothreitol (DTT), 1 g
Dithiothreitol (DTT), 5 g
2-Mercaptoethanol (BME), 25 ml
10% SDS Solution, 250 ml
10x TBE, 1 L
10x TBE, 5 L cube
10x Phosphate Buffered Saline (PBS), 1 L
10x Tris Buffered Saline (TBS), 1 L
10% Tween 20, 5 ml
10% Tween 20, 1 L
Blotting-Grade Blocker, nonfat dry milk, 300 g
1x PBS/1% Casein, 1 L
1x TBS/1% Casein, 1 L
Catalog #
List Price
EDU Price
161-0732EDU
161-0772EDU
161-0734EDU
161-0771EDU
161-0737EDU
161-0738EDU
161-0716EDU
161-0730EDU
161-0717EDU
161-0404EDU
161-0610EDU
161-0611EDU
161-0710EDU
161-0416EDU
161-0733EDU
161-0770EDU
161-0780EDU
170-6435EDU
166-2404EDU
161-0781EDU
170-6404EDU
161-0783EDU
161-0782EDU
$27.00
88.00
27.00
88.00
14.00
16.00
32.00
26.00
23.00
38.00
26.00
76.00
22.00
42.00
36.00
107.00
41.00
38.00
41.25
34.00
44.00
88.00
88.00
$21.60
70.40
21.60
70.40
11.20
12.80
25.60
20.80
18.40
30.40
20.80
60.80
17.60
33.60
28.80
85.60
32.80
30.40
33.00
27.20
35.20
70.40
70.40
170-3932EDU
162-0145EDU
166-2807EDU
162-0214EDU
170-3933EDU
170-4153EDU
170-4154EDU
46.00
60.00
71.25
128.00
37.00
32.00
39.00
36.80
48.00
57.00
102.40
29.60
25.60
31.20
Blotting Membranes and Trans-Blot® Turbo™ Packs
Thick Blot Paper, 50 sheets, 7.5 x 10 cm
0.45 µm Nitrocellulose, 7 x 8.4 cm, 10 sheets
0.45 µm Nitrocellulose (8 sheets)/Filter Paper Pack (16 sheets)
0.45 µm Nitrocellulose/Filter Paper Sandwich, 7 x 8.5 cm, 20 pk
Mini Trans-Blot Fiber Pads, 8 x 11 cm, 4
Trans-Blot™ Turbo™ Mini PVDF 2-Pack
Trans-Blot Turbo Midi PVDF 2-Pack
Buffers and reagents ship and store at room temperature. Store as directed on label. For more information on Criterion XT long shelf life
gels please visit us on the Web at discover.bio-rad.com.
124
Visit us on the Web at explorer.bio-rad.com
PROTEINS AND PROTEOMIC STUDIES: PRECAST GELS • ELECTROPHORESIS BUFFERS AND REAGENTS
Premixed Buffers for Electrophoresis
125
Proteins and Proteomic Studies: Protein Electrophoresis Standards and Stains
Bio-Rad’s protein electrophoresis standards and molecular weight markers provide an
excellent way for your students to monitor the progress of their runs and make valid
gel-to-gel comparisons. Protein standards are also used to estimate molecular weights
and identify proteins in polyacrylamide gels.
Bio-Rad protein standards contain bromophenol blue and glycerol, which allows
easier sample loading, prevents the proteins from freezing at –20°C, and eliminates
freeze/thaw degradation.
Prestained SDS-PAGE Standards
Prestained standards are used in SDS-PAGE and western blotting applications. They
allow continuous monitoring of protein separation during electrophoresis and provide
a quick and easy way to assess blotting efficiency.
Prestained standards are provided preblended in a stable sample buffer. No
reconstitution or further dilution is necessary before use. Prestained SDS-PAGE
standards are available in high, low, and broad molecular weight ranges so you can
select the range that is best for your application. The molecular weights of
each lot are individually calibrated and included with every vial.
Kaleidoscope™ Prestained Standards
Our most popular teaching lab standard, Kaleidoscope prestained standards contain
seven individually color-coded proteins, allowing real-time monitoring and instant band
recognition of proteins during and after electrophoresis. The molecular weights of
each lot are individually calibrated and included with every vial.
Precision Plus Protein™ Standards
Precision Plus Protein standards offer unsurpassed band sharpness, accurate molecular
weight estimations, lot-to-lot consistency, and instant orientation for gel electrophoresis
and western blotting. Precision Plus Protein standards contain ten highly purified
recombinant proteins, including three reference bands, in an ideal range of bands from
10 to 250 kD. Precision Plus Protein standards are available in Kaleidoscope, unstained,
all blue, and dual color options, providing four ways to get sharper-looking results.
Protein Electrophoresis Reagent Pack
Protein Stains
250 kD
Proteins in electrophoresis gels can be seen using a variety of protein-specific stains.
For more detailed information and specifications about the stains described below,
visit us on the Web at discover.bio-rad.com.
150
100
Bio-Safe™ Coomassie Stain
By far our most popular stain for research and teaching labs, Bio-Safe
Coomassie stain detects proteins in native, SDS-PAGE, and peptide gels
without methanol, acetic acid, or any other hazardous reagents. Bio-Safe
Coomassie stain is a ready-to-use, single-reagent protein stain that produces
a linear response, allowing quantitative analysis. Color development is extremely
easy. Simply soak the gel in the stain and rinse with water — results begin to appear
in 20 minutes. With sensitivity as low as 8 ng per band, you simply get better results
without the solvent waste and toxicity problems of conventional protein stains.
75
50
37
25
20
15
10
Precision
Plus Protein
Kaleidoscope
Standards
Precision
Plus Protein
All Blue
Standards
Myosin
216 kD
β-galactosidase
132
BSA
78
Carbonic anhydrase
45.7
Soybean trypsin inhibitor
32.5
Lysozyme
18.4
Ordering Information
Aprotinin
7.6
Kaleidoscope
Prestained
Standards
Myosin
Protein Electrophoresis Reagent Pack
Coomassie Brilliant Blue R-250 Solutions
Coomassie Brilliant Blue R-250 staining solution is the easiest way to fix and detect
proteins in polyacrylamide gels. Ready-to-use 1 L solutions eliminate the need to
weigh powders or dilute solutions.
Bio-Safe™ Coomassie Stain
The new SDS-PAGE Electrophoresis Module
provides all of the reliability of Bio-Rad’s
high-quality size standards, buffers, and
stains in a convenient reagent pack.
126
Precision
Plus Protein
Dual Color
Standards
Silver Stain Plus™ Kit
Silver Stain Plus stain is our most sensitive (ng) and easiest-to-use silver stain — just
3 simple steps complete in 1 hour. The Silver Stain Plus kit is ideal for both proteins
and nucleic acids in polyacrylamide or agarose gels and stain 40 mini gels per kit.
209 kD
β-galactosidase
124
BSA
80
Ovalbumin
49.1
Carbonic anhydrase
Soybean trypsin inhibitor
34.8
28.9
Lysozyme
20.6
Aprotinin
7.1
Prestained
Standards,
Broad Range
Description
Protein Standards
Prestained SDS-PAGE Standards, low range, 500 µl
Prestained SDS-PAGE Standards, high range, 500 µl
Prestained SDS-PAGE Standards, broad range, 500 µl
Kaleidoscope Prestained Standards, 500 µl
Precision Plus Protein Unstained Standards, 1,000 µl
Precision Plus Protein All Blue Standards, 500 µl
Precision Plus Protein Dual Color Standards, 500 µl
Precision Plus Protein Kaleidoscope Standards, 500 µl
SDS-PAGE Electrophoresis Module, includes 1L 10x
TGS buffer, 30 ml Laemmli sample buffer, 1L Bio-Safe
Coomassie stain, and 500 µl Precision Plus Protein Dual
Color standards
Protein Stains
Bio-Safe Coomassie Stain, 1 L
Bio-Safe Coomassie Stain, 5 L
Silver Stain Plus Kit
Coomassie Brilliant Blue R-250 Kit,
includes 1 L stain solution, 2 L destain solution
Catalog #
List Price
EDU Price
161-0305EDU
161-0309EDU
161-0318EDU
161-0324EDU
161-0363EDU
161-0373EDU
161-0374EDU
161-0375EDU
166-5060EDU
$86.00
94.00
100.00
115.00
91.00
97.00
120.00
130.00
218.75
$68.80
75.20
80.00
92.00
72.80
77.60
96.00
91.00
175.00
161-0786EDU
161-0787EDU
161-0449EDU
161-0435EDU
76.00
292.00
205.00
123.00
60.80
233.60
164.00
98.40
Best Selection at the Lowest Prices
TGX and Criterion precast gels are
available in a wide selection of
formulations for SDS-PAGE, native
PAGE, and peptide separations. For a
complete listing, visit us on the Web
at discover.bio-rad.com. For more
information, request bulletins 5871
(Mini-PROTEAN TGX Precast Gels),
and 4110001 (Instruction Manual,
Criterion Gel Application Guide).
PROTEINS AND PROTEOMIC STUDIES: PROTEIN ELECTROPHORESIS STANDARDS AND STAINS
Protein Electrophoresis Size Standards
Protein standards are shipped at room temperature. Store in freezer upon arrival.
Mini-PROTEAN® TGX™ precast gels, p. 123
Criterion™ precast midi gels, p. 123
Mini-PROTEAN® Tetra electrophoresis cell, p. 120
Criterion cell, p. 120
Electrophoresis buffers, p. 124–125
Visit us on the Web at explorer.bio-rad.com
127
Molecular Biology Laboratory: Equipment and Supplies
Section Contents
PowerPac™ Power Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130
Spectrophotometer, Gel Imaging, and Documentation
Spectrophotometer and Cuvettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
Gel Imaging and Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132
UV Illumination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
Microplate Absorbance Reader and Software . . . . . . . . . . . . . . . . . . . . . 133
Centrifuges
Model 16K Microcentrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
Mini Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
Mixing Devices
Tube Roller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
BR-2000 Vortexer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
Mini Rocker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
UltraRocker™ Rocking Platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136
Temperature Control Devices
Digital Dry Bath . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136
DyNA Chill Cooler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136
Mini Incubation Oven . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
Water Bath . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
Micropipets and Tips
Micropipets, Multichannel Pipet, and Micropipet Racks . . . . . . . . . . . . . . 138
Professional Pipet Backpack Starter Kit and Pipet Controller . . . . . . . . . . 139
Pipet Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140
Chromatography Reagents and Equipment
Chromatography Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141
BioLogic™ Chromatography Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141
Laboratory Storage and Plastic Refresh Consumables
PROTEINS AND PROTEOMIC STUDIES: EQUIPMENT AND REAGENTS
Electrophoresis Power Supplies
Racks and Storage Boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142
Plastic Refresh Kit Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143
1>
3>
2>
128
4>
1. Valley Christian High School, San Jose, California; 2. University of California, Davis, California;
3. Independence High School, Thompson’s Station, Tennessee; 4. Sera Jey Monastery Workshop,
Bylakuppe, India.
129
Molecular Biology Laboratory: Electrophoresis Power Supplies • Spectrophotometer • Cuvettes
SmartSpec™ Plus Spectrophotometer
Boost classroom performance and energize your teaching lab with Bio-Rad’s PowerPac
power supplies.
The SmartSpec Plus spectrophotometer has more features and functionality than
many other benchtop spectrophotometers. Combining performance and stability
at an affordable price, it is perfect for protein assays, quantitation of DNA,
and monitoring cell culture growth.
No power supply offers better value and performance than the PowerPac series. Decades
of experience in electrophoresis went into developing Bio-Rad’s PowerPac power supplies.
Designed to fit the complete range of teaching and research applications, all PowerPac
power supplies are IEC 1010-1 certified, making Bio-Rad’s the safest and most reliable
electrophoresis power supplies in the world.
PowerPac Mini Power Supply
(available in the U.S. only)
PowerPac™ Mini Power Supply (U.S. Only)
The PowerPac Mini is our lowest-priced power supply. This dedicated little workhorse
provides the energy needed to run horizontal agarose or vertical polyacrylamide gel
electrophoresis. Simply set the voltage and run.
PowerPac™ Basic Power Supply
The PowerPac Basic power supply is designed for horizontal agarose gel electrophoresis
of DNA and vertical acrylamide gel electrophoresis of proteins. Its design provides
simple programming and a compact, stackable case. The PowerPac Basic offers timer
control, constant voltage or constant current output, real-time monitoring of both
voltage and current, and pause/resume run capability.
For more information on the complete PowerPac power supply series, including
the PowerPac HC, HV, and Universal power supplies, please visit us at
www.bio-rad.com/en-us/product/power-supplies.
PowerPac HC
Power Supply
PowerPac Basic
Power Supply
•
•
•
•
•
•
•
•
•
Built-in printer
Long-life xenon flash lamp
User interface with six different languages
Easy-to-use, menu-driven operation
Compact, space-saving design
Performs assays from 200 to 800 nm
Scans wavelengths for spectral analysis
Constructs and saves standard curves
Performs simple kinetic assays
Specifications
Wavelength range
Light source
Absorption accuracy
Dimensions
PowerPac HV
Power Supply
PowerPac Universal
Power Supply
Specifications
PowerPac Mini
PowerPac Basic
PowerPac HC
PowerPac Universal
PowerPac HV
Output range
100 V or 200 V
constant
10–300 V, fully adjustable
in 1 V steps
5–250 V
in 1 V steps
10–500 V
in 1 V steps
20–5,000 V
in 1 V steps
Programmability
No
Yes
Yes
Yes
Yes
Current
200 mA maximum
4–400 mA, fully adjustable
in 1 mA steps
0.01–3.0 A
in 0.01 A steps
0.01–2.5 A
in 1 mA steps
1–500 mA
in 1 mA steps
Power
40 W maximum
75 W maximum
300 W maximum
500 W maximum
400 W maximum
Type of output
Constant voltage
Constant voltage or
constant current with
automatic crossover
Constant voltage,
current, or power
Constant voltage,
current, or power
Constant voltage,
current, or power
Display
NA
LED
LCD
Backlit graphic LCD
Backlit graphic LCD
Temperature control
NA
NA
NA
NA
Optional probe
available
Operating range
0–40°C
0–40°C
0–40°C
0–40°C
0–40°C
Dimensions (W x D x H)
15.5 x 11 x 6 cm
21 x 24.5 x 6.5 cm
25 x 28.5 x 8 cm
27.5 x 34 x 10 cm
27.5 x 34 x 10 cm
Weight
0.5 kg
1.1 kg
2.0 kg
2.5 kg
2.5 kg
200–800 nm
Xenon lamp
±0.01 @ 0.5 AU
±0.02 @ 1.0 AU
33 x 26 x 14 cm (W x D x H)
trUView™ Cuvettes
Bio-Rad’s disposable trUView cuvettes allow accurate and precise
quantitation of DNA, RNA, and proteins. Made from highly transparent plastic resin,
trUView cuvettes minimize absorption of UV light and eliminate the need for expensive
quartz cuvettes.
Standard Polystyrene Cuvettes
Bio-Rad’s standard disposable polystyrene cuvettes are ideal for use with the Bio-Rad
protein assay. Assays can be mixed directly in the cuvettes. For more information
on Bio-Rad’s complete line of protein assay kits, request bulletin 1069 or go to
www.bio-rad.com/proteinassays.
Cuvettes
Additional supplies:
• Cuvette Racks, p. 142
Ordering Information
Description
Ordering Information
Description
PowerPac Power Supplies
PowerPac Mini Power Supply, 120 (U.S. only)
PowerPac Basic Power Supply, 120/220 V
PowerPac HC Power Supply, 120/220 V
PowerPac Universal Power Supply, 120/220 V
PowerPac HV Power Supply, 120/220 V
PowerPac HV Power Supply with Temperature Probe
Catalog #
List Price
EDU Price
165-5048EDU
164-5050EDU
164-5052EDU
164-5070EDU
164-5056EDU
164-5059EDU
$327.14
350.00
800.00
2,331.00
2,280.00
2,670.00
$229.00
280.00
640.00
1,864.08
1,824.00
2,136.00
SmartSpec Plus Spectrophotometer
(see p. 142 for cuvette racks)
SmartSpec Plus Spectrophotometer and Cuvettes
SmartSpec Plus Spectrophotometer
trUView Cuvettes, 50
trUView Cuvettes, 100
trUView Cuvette height adaptor, 15 mm
3.5 ml Standard Disposable Polystyrene Cuvettes, 100
1.5 ml Standard Disposable Polystyrene Cuvettes, 100
Standard Cuvette, 3.5 ml, quartz
Semimicrovolume Cuvette, 1.4 ml, quartz
SmartSpec Printer Paper, 5 rolls
Catalog #
List Price
EDU Price
170-2525EDU
170-2510EDU
170-2511EDU
170-2512EDU
223-9950EDU
223-9955EDU
170-2502EDU
170-2503EDU
170-2506EDU
$5,893.00
62.00
133.00
37.00
12.00
12.00
174.00
261.00
41.00
$4,714.40
49.60
106.40
29.60
9.60
9.60
139.20
208.80
32.80
SmartSpec Plus
Spectrophotometer supports
the following Biotechnology
Explorer kits:
• Got Protein? Kit (#166-2900EDU)
• Biofuel Enzyme Kit (#166-5035EDU)
• Cloning and Sequencing Explorer
Series (see pp. 66– 75)
• Protein Expression and Purification
Series (see pp. 76–81)
For more detailed information about the SmartSpec Plus spectrophotometer and Bio-Rad’s protein assay kits,
visit us on the Web at explorer.bio-rad.com and request bulletins 2826 and 1069.
For more detailed specifications about power supplies, visit us on the Web at explorer.bio-rad.com.
130
Visit us on the Web at explorer.bio-rad.com
MOLECULAR BIOLOGY LABORATORY: ELECTROPHORESIS POWER SUPPLIES • SPECTROPHOTOMETER • CUVETTES
PowerPac™ Power Supplies
131
Molecular Biology Laboratory: Gel Imaging and Documentation • UV Illumination • Microplate
Reader
UV Illumination
Bio-Rad offers a complete suite of imaging systems for detecting, imaging, and quantitating
colorimetric, chemiluminescent, fluorescent, and radioisotopic signals. Software provides
automation for image acquisition with data analysis and validation. For more information
visit us on the Web at discover.bio-rad.com and download bulletin 5888.
UV Pen Light
This pocket-sized little disposable lamp has the power needed to illuminate your
experiments. The wavelength is optimized for your green fluorescent protein activities,
including our pGLO™ bacterial transformation, GFP chromatography, and Secrets of
the Rainforest™ kits.
New UView™ Mini Transilluminator
UView mini transilluminator is a small-format transilluminator that has an excitation peak
of 365 nm and works well for visualizing DNA fragments when using the UView 6x loading
dye (p.109) and others. The unit has a UV blocking shield and an auto shutoff when the
shield is removed in order to reduce accidental UV exposure. The shutoff can be manually
overridden to facilitate cutting bands out of gels while wearing appropriate UV protective
wear. The viewing area can accommodate one mini gel lengthwise or two mini gels
widthwise (viewing area is 8 x 15 cm).
Gel Doc™ EZ Imaging System
The name says it all. The Gel Doc EZ imaging system is a compact, automated imaging
system that gets you to your image in three easy steps: load your gel, push the button,
and the results are automatically calculated and displayed. The system comes with intuitive
Image Lab™ software, making imaging and analysis incredibly easy, and one stain-free
sample tray, allowing you to visualize your Mini-Protean® TGX Stain-Free™ Precast gels.
Other trays available include a UV tray (for ethidium bromide staining of DNA gels and
fluorescence imaging), a white tray (for Coomassie, copper, silver, and zinc stains), and
a blue tray (for nucleic acid applications that use SYBR® stains).
Gel Doc EZ System Features:
•
•
•
•
The New UView Mini Transilluminator
UView Mini Transilluminator
Specifications
Illumination type
UV lamp
Wavelength
365 nm
Dimensions
(L x W x H)
24.7 x 13.2 x 4.9 cm
Viewing surface
15.0 x 8 cm
Weight
1.4 kg
Compatible gel
sizes
5 x 6 cm, 10.5 x 6 cm,
12.5 x 6 cm
Power
12 V, 1.5 A
Input
115–240 V, 4 different
country adapters included
Safety
Auto shut off when lid
is opened, capable of
manual override
Long-Wave UV Lamp
This convenient, handheld long-wave UV lamp runs on four AA batteries and has
a wavelength output between 300 and 400 nm. Designed for excitation of green
fluorescent protein (GFP), this lamp fits the bill for our pGLO™ bacterial transformation,
GFP chromatography, pGLO kit SDS-PAGE extension, and Secrets of the Rainforest
kits. The acrylic safety shield protects the bulb and the user’s eyes.
The iMark microplate absorbance reader is an economical, high-performance absorbance
reader with flexible configurations. Superior onboard software provides user log-in, plenty
of room for protocols and data storage, and sufficient curve-fit and reporting functions.
Bio-Rad’s microplate readers can be utilized to their full potential with Microplate
Manager® software. This sophisticated software allows powerful, versatile colorimetric
and turbidimetric analyses, as well as report analysis for raw data, absorbance, limit,
matrix, normalization, and curve fit.
For more information, visit us on the Web at discover.bio-rad.com.
Capability to read flat-, U-, or V-bottom microplates, or 8- or 12-well strip plates
Single or dual wavelength detection
Variable-speed plate shaking capability
Easy-access 8-position filter disk, with four standard filters provided
Integrated thermal printer
USB2 port for external PC control
Optional Microplate Manager software
Specifications
Ordering Information
Description
Catalog #
List Price
EDU Price
166-0531EDU
$1,120.00
$896.00
170-8270EDU
7,500.00
6,000.00
UV Sample Tray
White Light Sample Tray
Blue Light Sample Tray
Stain-Free Sample Tray
Sample Tray Holder
170-8271EDU
170-8272EDU
170-8273EDU
170-8274EDU
170-8276EDU
1,000.00
1,000.00
1,000.00
1,000.00
290.00
800.00
800.00
800.00
800.00
232.00
Mitsubishi P93DW Printer
Mitsubishi Thermal Printer Paper
170-8089EDU
170-7581EDU
1,552.00
143.00
1,241.60
114.40
UView
UView mini transilluminator
Gel Doc EZ System and Accessories
Gel Doc EZ System for Mac or PC includes: darkroom,
camera, cables, Image Lab software, and stain-free tray
(other trays sold separately)
Molecular Imager® Gel Doc™ XR+ Documentation System and Accessories
Gel Doc XR+ with Image Lab System for PC/Mac, includes
170-8195EDU
11,665.00
universal hood, camera, cables, and installation kit
9,332.00
Image Lab Upgrade Kit, includes focus calibration target,
lens flat field disk, Image Lab CD, and instructions
170-8198EDU
1,050.00
840.00
UV/White Light Conversion Screen
White-Light Transilluminator, for use with Gel Doc XR only
170-8289EDU
170-7950EDU
610.00
615.00
488.00
492.00
The Gel Doc EZ Imaging System
(computer not included)
Gel Doc EZ supports the
following Biotechnology
Explorer™ kits:
• Forensic DNA fingerprinting kit
(#166-0007EDU)
• Lambda DNA kits (see pp. 52– 53)
• PCR Amplification kits (see pp. 54– 65)
• pGLO™ kit SDS-PAGE extension
(#166-0013EDU)
• Comparative proteomics kits I and II
and V3 Western Workflow™:
(see pp. 38–43)
• Cloning and Sequencing Explorer
Series (see pp. 66– 75)
• Protein Expression and Purification
Series (see pp. 76–81)
Long-Wave UV Lamp
iMark™ Microplate Absorbance Reader
•
•
•
•
•
•
•
Easy to use — no need for manual control of filter, lights, or lenses
Time-saving — get results quickly
Space-saving — small footprint: 43 x 28 x 38 cm (H x W x D)
Modular design and flexible options — application-specific trays allow you to
configure your system and upgrade when you want to
UV Pen Light
Wavelength range
OD range
Accuracy
Resolution
Dimensions
400–750 nm
0–3.500 OD
±1% at 490 nm
0.001 OD
34.6 x 37.7 x 16.4 cm (W x D x H)
iMark Microplate Absorbance Reader
iMark Microplate Absorbance
Reader supports the following
Biotechnology Explorer™ kits:
• ELISA Immuno Explorer™ kit
(#166-2400EDU)
• Got Protein?™ Kit (#166-2900EDU)
• Biofuel Enzyme Kit (#166-5035EDU)
Ordering Information
Description
Catalog #
List Price
EDU Price
166-0530EDU
166-0500EDU
$13.75
43.75
$11.00
35.00
iMark Microplate Absorbance Reader and Accessories
iMark Microplate Absorbance Reader, 100/240 V, includes 6 filters (415, 450, 490, 595, 655, and 750 nm),
plate shaker, onboard software and thermal printer, one roll printer paper, USB, power cables, and instructions
168-1130EDU
5,460.00
4,368.00
UV Lamps
Long-Wave UV Pen Light
Long-Wave UV Lamp, requires 4 AA batteries
iMark Microplate Absorbance Reader, with Microplate Manager software
168-1135EDU
6,425.00
5,140.00
Microplate Manager Software, version 6.1, PC or Mac, for end-point and kinetic data analysis with
microplate absorbance readers
168-9520EDU
1,945.00
1,556.00
PC Serial Cable for iMark Microplate Reader
Printer Paper for iMark Microplate Reader, 2 rolls
Microplates, 96-well, EIA, polystyrene, 100
168-1005EDU
168-1007EDU
224-0096EDU
120.00
52.00
353.00
96.00
41.60
282.40
For more information on the Molecular Imager Gel Doc XR+ Documentation System, visit us the on the Web at
bio-rad.com/imagingsystems.
132
Visit us on the Web at explorer.bio-rad.com
MOLECULAR BIOLOGY LABORATORY: GEL IMAGING & DOCUMENTATION • UV ILLUMINATION • MICROPLATE READER
Gel Imaging and Documentation
133
Molecular Biology Laboratory: Centrifuges • Mixing Devices
Mixing Devices
Model 16K Microcentrifuge
The Model 16K, the ultimate benchtop centrifuge, is designed for all your teaching
lab applications. The motor is brushless, exceptionally quiet, and requires no
routine maintenance.
Tube Roller
The tube roller is simple, yet versatile — allowing for mixing of samples both
horizontally and vertically. Its compact design allows it to fit inside our mini
incubation oven, making it ideal for mixing liquids while incubating.
• Quick-spin features
• Safe for coldroom operation
•
•
•
•
•
•
Model 16K
Microcentrifuge
Specifications
Max. speed
Capacity
Safety
Automation
Dimensions
Regulatory certification
14,000 rpm (16,000 x g)
18-place rotor for 1.5 and 2.0 ml tubes
Safety interlock
30 minute timer or quick-spin button
21 x 23 x 18 cm (W x D x H)
CE compliant
Tube Roller
Specifications
Capacity – standard
Model 16K Microcentrifuge PCR Tube Adaptor
This adaptor holds two PCR 8-tube strips or up to 16 individual 0.2 ml tubes.
The adaptor fits on top of the standard 18-place rotor and is easily attached to
and removed from the rotor. The PCR tube adaptor is sold separately.
Model 16K Microcentrifuge
PCR Tube Adaptor (sold separately)
Mini Centrifuge
This economical and reliable mini centrifuge handles most teaching lab
applications, including quick sample spin-downs and cell pelleting. This unit
is provided with a microtube rotor, a PCR strip tube rotor, and adaptors for
0.4 and 0.5 ml tubes.
Speed
Operating range
Maximum load
Dimensions
Regulatory certification
36 x 1.5/2.0 ml tubes,
10 x 15 ml tubes, or
6 x 50 ml tubes
24 rpm
4–65°C
0.8 kg (1.75 lb)
21.3 x 10.2 x 12.6 cm
(W x D x H)
CE compliant
BR-2000 Vortexer
The BR-2000 vortexer is the general-purpose mixer for your varied laboratory
needs. The three-position switch allows a range of applications, from gentle
sample mixing to resuspending cell pellets. The mixer can be operated
continuously or by touch activation. This heavy-duty mixer is equipped with
rubber feet to avoid sliding even at maximum speed. The BR-2000 vortexer
is supplied with a general-purpose cup attachment; the flathead dimpled
adaptor, useful for mixing larger volumes, is sold separately.
Specifications
Max. speed
Capacity
Dimensions
Regulatory certification
Compact design
Wide selection of motions
Noiseless operation
Coldroom compatible
Dual-format rotisseries
Includes three easily interchangeable rotisseries
6,000 rpm (2,000 x g)
6 x 1.5/2.0 ml or 0.4/0.5 ml tubes, 16 x 0.2 ml PCR tubes
15 x 15 x 11 cm (W x D x H)
CE compliant
Specifications
Mini Centrifuge
Ordering Information
Description
Microcentrifuge and Mini Centrifuge
Model 16K Microcentrifuge, 120 V
Model 16K Microcentrifuge, 220 V
PCR Tube Adaptor, for Model 16K microcentrifuge
Mini Centrifuge, 120 V
Mini Centrifuge, 220 V
Mini Centrifuge, 220 V, for the UK
134
Catalog #
List Price
EDU Price
166-0602EDU
166-0612EDU
166-0620EDU
166-0603EDU
166-0613EDU
166-0623EDU
$2,768.58
Inquire
149.34
427.14
Inquire
Inquire
$1,938.01
Inquire
112.01
299.00
Inquire
Inquire
Speed range
Operating modes
Operating range
Dimensions
Regulatory certification
0–3,000 rpm
Continuous operation or touch control
4–65°C
12 x 15 x 13 cm (W x D x H)
CE compliant
BR-2000 Vortexer
Flathead
Dimpled Adaptor
(sold separately)
MOLECULAR BIOLOGY LABORATORY: CENTRIFUGES • MIXING DEVICES
Centrifuges
Mini Rocker
The Mini Rocker provides the best three-dimensional mixing action. Gentle yet
thorough mixing makes it perfect for western blot incubations and staining of
gels. Its compact design allows it to fit inside our mini incubation oven for
temperature-controlled mixing.
• Designed for processing blots and staining gels
• Optimal fixed speed and tilt
• 3-dimensional motion
Specifications
Tube Roller
Tube Roller, 120 V, includes 3 tube carousels for 1.5, 15, and 50 ml tubes
Tube Roller, 230 V, for Europe, includes 3 tube carousels for 1.5, 15, and 50 ml tubes
Tube Roller, 230 V, for the UK, includes 3 tube carousels for 1.5, 15, and 50 ml tubes
166-0711EDU
166-0721EDU
166-0722EDU
712.87
Inquire
Inquire
499.01
Inquire
Inquire
Vortexer
BR-2000 Vortexer, 120 V
BR-2000 Vortexer, 220 V
BR-2000 Vortexer, 220 V, for the UK
Flathead Dimpled Adaptor
166-0610EDU
166-0611EDU
166-0621EDU
166-0622EDU
407.15
Inquire
Inquire
71.44
285.01
Inquire
Inquire
50.01
Mini Rocker
Mini Rocker, 120 V, includes 2 blotting boxes
Mini Rocker, 230 V, for the UK and Europe, includes 2 blotting boxes
166-0710EDU
166-0720EDU
592.86
Inquire
415.00
Inquire
Speed
Motion/Pitch
Maximum load
Operating range
Dimensions
Capacity
Regulatory certification
24 rpm
3-D/fixed 5º
0.8 kg (1.75 lb)
4–40ºC
20.3 x 17.8 x 9.5 cm (W x D x H)
Four 8 x 10 cm or two 10 x 20 cm blotting boxes
CE compliant
Mini Rocker
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135
Molecular Biology Laboratory: Rocker • Digital Dry Bath • Cold Storage • Incubation Oven
Water Bath
Mini Incubation Oven
Simply the most economical and reliable incubator, this compact oven (0.5 cu. ft.) is
thermostatically controlled for growth of bacterial cultures over a wide range of temperatures.
The oven is now available with an accessory port in the rear of the unit to allow for the insertion
of our tube roller (1.5/2.0 ml, 15 ml, and 50 ml tube options) or mini rocker for temperaturecontrolled mixing. This mini incubation oven will support a variety of classroom experiments.
UltraRocker™ Rocking Platform
This versatile rocking platform meets the demands of many molecular biology applications.
The two platforms are corrosion resistant and are lined with a nonslip rubber mat to keep
containers in place. The tilt capacity and variable speed control of this rocker permit a
broad range of mixing and agitation options for the following applications:
Specifications
• Overnight incubation of liquid bacterial cultures
• Staining and destaining of agarose and polyacrylamide gels and blots
• Southern hybridization
Temp. range
Temp. uniformity
Capacity
Dimensions
Exterior
Chamber
Regulatory certification
Specifications
Speed range
Motion
Platform dimensions
Platform clearance
Dimensions with double platform
Maximum load
Operating range
Regulatory certification
8–40 rpm
Tilting, ±7.5°
29 x 21 cm (W x D)
9 cm
29 x 21 x 25 cm (W x D x H)
4.5 kg (10 lb)
4–80°C
CE compliant
UltraRocker Rocking Platform
Mini Incubation Oven
28 x 29 x 34 cm (W x D x H)
23 x 20 x 20 cm (W x D x H)
CE compliant
Water Bath
This temperature-controlled water bath is hot! It’s built for accuracy, dependability, affordability,
and safety.
• Easy-to-clean, seamless stainless-steel tank
• Stainless-steel gable cover
• Electrostatically applied finish that resists rust, corrosion,
and scratches
• Over-temperature protection
• Thermometer
Temperature Control Devices
Digital Dry Bath
This digitally controlled dry bath is perfect for a multitude of laboratory
procedures where incubation of samples is needed. It’s accurate and
built for safe continuous operation. It’s economical and versatile, too!
•
•
•
•
•
Room temperature to 60°C
±5°C
Eighty 6.5 cm plates
Specifications
Base unit includes one heat block for 1.5 ml tubes
Microprocessor control with digital display
Easy user calibration
Holds 24 microcentrifuge tubes
Optional blocks for 0.5, 2.0, and 15 ml tubes
Digital Dry Bath
1.5 ml Block
Temperature range
Capacity
Dimensions
Exterior
Interior
Regulatory certification
Room temperature to 100°C
6L
36 x 28 x 32 cm (W x D x H)
32 x 17 x 18 cm (W x D x H)
CE compliant
Water Bath
Specifications
Temperature range
Temp. display resolution
Temp. uniformity
Temp. accuracy
Block chamber
Single block unit
Dimensions
Footprint
Regulatory certification
Dry Bath Block
Construction
Thermometer well
Block capacity
Room temperature +5º to 150ºC
0.1ºC, 4 digit LED
±0.2ºC at 37ºC
±0.3ºC
Stainless steel
One standard block
20 x 28.4 x 8.4 cm (W x D x H)
20 x 28.4 cm
CE compliant
Ordering Information
Description
Rocking Platform
UltraRocker Rocking Platform, 120 V
UltraRocker Rocking Platform, 220 V
0.5 ml Block
2.0 ml Block
15 ml Block
High-grade, nonporous aluminum with
anodized surface
Required only for calibration purposes
24 x 0.5 ml tubes, 24 x 1.5 ml tubes,
24 x 2.0 ml tubes, or 12 x 15 ml tubes
DyNA Chill Cooler
Why struggle with a bucket of ice? This handy little cooler keeps your precious samples
chilled for you. Simply chill the DyNA Chill cooler at your chosen temperature overnight and
then you’re ready to go without the mess of ice.
• Keeps samples cold between –15°C and room temperature
• Keeps 1.5–2.0 ml microtube samples cold for 8 hours without ice
DyNA Chill Cooler
Specifications
Capacity
Temperature range
Storage time
136
12 x 1.5/2.0 ml tubes
–15°C to room temperature
Up to 8 hours at –15°C
Catalog #
List Price
EDU Price
166-0709EDU
166-0719EDU
$1,141.43
Inquire
$799.00
Inquire
Digital Dry Bath
Digital Dry Bath, 120 V, includes 1.5 ml heating block
Digital Dry Bath with all 4 heating blocks, 120 V, includes 0.5, 1.5, 2.0, and 15 ml heating blocks
Digital Dry Bath, 230 V, for the UK and Europe, includes 1.5 ml heating block
Digital Dry Bath with all 4 heating blocks, 230 V, for the UK and Europe, includes 0.5, 1.5, 2.0, and 15 ml
heating blocks
Digital Dry Bath heating block, 0.5 ml, for 24 x 0.5 ml tubes
Digital Dry Bath heating block, 2.0 ml, for 24 x 2.0 ml tubes
Digital Dry Bath heating block, 15 ml, for 12 x 15 ml tubes
Digital Dry Bath heating blocks, 3 pack, 0.5 ml, 2.0 ml, and 15 ml blocks
166-0562EDU
166-0571EDU
166-0563EDU
166-0572EDU
642.86
953.34
Inquire
Inquire
450.00
686.40
Inquire
Inquire
166-0565EDU
166-0566EDU
166-0567EDU
166-0570EDU
122.50
122.50
122.50
366.67
96.78
96.78
96.78
275.00
Mini Incubation Oven
Mini Incubation Oven, 120 V
Mini Incubation Oven, 220 V
Mini Incubation Oven and Mini Rocker, 120 V
Mini Incubation Oven and Tube Roller, 120 V
166-0501EDU
166-0521EDU
166-0712EDU
166-0713EDU
557.15
Inquire
1,128.58
1,242.86
390.01
Inquire
790.01
870.00
Water Bath
Temperature-Controlled Water Bath, 120 V
Temperature-Controlled Water Bath, 220 V
166-0504EDU
166-0524EDU
781.25
Inquire
546.88
Inquire
DyNA Chill Cooler
DyNA Chill Cooler
166-0564EDU
107.14
75.00
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MOLECULAR BIOLOGY LABORATORY: ROCKER • DIGITAL DRY BATH • COLD STORAGE • INCUBATION OVEN • WATER BATH
Mixing Devices (continued)
•
137
Molecular Biology Laboratory: Micropipets • Micropipet Racks • Pipet Controller
Professional Micropipet Backpack Starter Set
The professional micropipet backpack starter set is a complete
liquid handling package that includes everything needed to get
started with accurate and reproducible pipetting. Exceptional
value includes:
The procedures used in molecular biology require precise manipulation of small
volumes of solutions containing DNA, proteins, buffers, or enzymes. Micropipets are
required to accurately measure and transfer solution volumes in microliter (µl) ranges.
No biology teaching lab experience is complete without hands-on use of micropipets.
Fixed-Volume Micropipets
Get all the accuracy of standard adjustable-volume micropipets — but at a fraction of
the price! These dedicated pipets accommodate sample volumes most frequently
encountered in a teaching lab. Using standard pipet tips, each color-coded pipet will
transfer a fixed volume of solution: 5 µl (red), 10 µl (green), 20 µl (yellow), or 50 µl (blue).
Fixed-Volume Micropipets
Classroom Digital Micropipets
These adjustable pipets deliver exceptional classroom performance. Four models are
available: 0.5–10 µl, 2–20 µl, 20–200 µl, and 100–1,000 µl. Features include:
•
•
•
•
•
•
•
2-year warranty
Adjustable digital dial with convenient tip ejector
Accommodates standard pipet tips
0.1 µl volume increments for 0.5–10 µl pipets
0.5 µl volume increments for 2–20 µl pipets
1 µl volume increments for 20–200 µl pipets
5 µl volume increments for 100–1,000 µl pipets
Professional Pipet Controller
The pipet controller is designed for operation with glass
or plastic serologic pipets from 0.1 to 100 ml. With a slim,
ergonomic handle design, the controller is comfortable and
easy to use. It comes with a charger, bench stand, wall mount,
and 0.45 µm filter.
New 8-Channel Professional Micropipets
Multichannel pipets offer an easy way to enjoy the efficiency of pipetting multiple
samples at once. A great way to teach advanced pipetting skills for workforce
development coursework. Each channel has an independent precision piston assembly
to ensure accuracy and reproducibility from one pipetting series to the next as well as
between channels. Features include:
Pipet Controller
(serologic pipet not included)
Ordering Information
Description
Professional Adjustable-Volume Micropipets
New 8-Channel Professional
Micropipet (Pipet tips not included)
• Precision designed tip cones provide leak-proof tip fit
• Continuously adjustable volume selection (5–50 µl or 20 –200 µl) with thumbwheel
or push button
• Curved ejector bar reduces ejection force
• Fully autoclavable
138
Catalog #
Micropipet Rack
Fits nine digital
micropipets
Carousel Pipet Rack
Fits six digital micropipets
(not included)
List Price EDU Price
Classroom sets of 8 micropipets
Catalog #
List Price EDU Price
Fixed-Volume Micropipets
5 µl Fixed-Volume Pipet, red
10 µl Fixed-Volume Pipet, green
20 µl Fixed-Volume Pipet, yellow
50 µl Fixed-Volume Pipet, blue
166-0511EDU
166-0512EDU
166-0513EDU
166-0515EDU
$41.67
41.67
41.67
41.67
$25.00
25.00
25.00
25.00
166-0511BLK
166-0512BLK
166-0513BLK
166-0515BLK
$283.34
283.34
283.34
283.34
$170.00
170.00
170.00
170.00
Classroom Digital Micropipets
0.5–10 µl Digital Micropipet
2–20 µl Digital Micropipet
20–200 µl Digital Micropipet
100–1,000 µl Digital Micropipet
166-0550EDU
166-0551EDU
166-0552EDU
166-0553EDU
180.00
180.00
180.00
180.00
135.00
135.00
135.00
135.00
166-0550BLK
166-0551BLK
166-0552BLK
166-0553BLK
1,333.34
1,333.34
1,333.34
1,333.34
1000.01
1000.01
1000.01
1000.01
Professional Adjustable-Volume Digital Micropipets
0.1–2 µl Digital Micropipet
0.5–10 µl Digital Micropipet
2–20 µl Digital Micropipet
20–200 µl Digital Micropipet
100–1,000 µl Digital Micropipet
Professional Multichannel Pipet, 8-channel, adjustable volume, 5–50 µl
Professional Multichannel Pipet, 8-channel, adjustable volume, 20–200 µl
166-0499EDU
166-0505EDU
166-0506EDU
166-0507EDU
166-0508EDU
166-0496EDU
166-0495EDU
337.50
337.50
337.50
337.50
337.50
690.00
690.00
270.00
270.00
270.00
270.00
270.00
552.00
552.00
166-0499BLK
166-0505BLK
166-0506BLK
166-0507BLK
166-0508BLK
—
—
2,375.00
2,375.00
2,375.00
2,375.00
2,375.00
—
—
1,900.00
1,900.00
1,900.00
1,900.00
1,900.00
—
—
Micropipet Racks
Micropipet Rack, holds 9 single micropipets
Carousel Pipet Rack, holds 6 single professional micropipets
166-0554EDU
166-0487EDU
40.00
156.25
32.00
125.00
—
—
—
—
—
—
1,187.50
950.00
—
—
—
166-0490EDU
368.75
295.00
—
—
—
166-0491EDU
Inquire
Inquire
—
—
—
166-0492EDU
Inquire
Inquire
—
—
—
166-0493EDU
43.75
35.00
—
—
—
Professional Pipet Backpack Starter Set
Set includes four professional adjustable-volume digital micropipets
166-0486EDU
(0.5–10 µl, 2–20 µl, 20–200 µl, and 100 –1,000 µl),4 racks of pipet tips
(TBR-14, -35, -40, Prot/Elec), Carousel Pipet Rack, and Bio-Rad backpack
Micropipet Rack
Designed for the lab benchtop with wipe-and-clean surface and a non-skid base.
Carousel Pipet Rack
Six-place carousel rack conveniently rotates for easy access to your professional
micropipets.
Professional Micropipet Backpack Starter Set
(actual backpack provided may vary)
Classroom
Digital Micropipets
Professional Adjustable-Volume Digital Micropipets
Designed and engineered for research, these adjustable pipets deliver exceptional
comfort, durability, and performance. Guaranteed to function efficiently and reliably
for years — they’re the real thing. Five models are available to cover all applications
requiring transfers of small volumes of precious solutions: 0.1– 2 µl, 0.5 –10 µl,
2 – 20 µl, 20 – 200 µl, and 100 –1,000 µl. Features include:
• 3-year warranty
• Now fully autoclavable micropipet that accommodates standard pipet tips
• Adjustable digital dial with locking mechanism, slender contoured grip, and
ergonomic tip ejector
• 0.002 µl volume increments for 0.1– 2 µl pipets
• 0.02 µl volume increments for 0.5 –10 µl, 2–20 µl pipets
• 0.2 µl volume increments for 20 –200 µl pipets
• 2 µl volume increments for 100 –1,000 µl pipets
• 4 professional adjustable-volume digital micropipets
(0.5 –10 µl, 2–20 µl, 20–200 µl, and 100 –1,000 µl)
• Bio-Rad pipet tips
(four racks including TBR-14, -35, -40, and Prot/Elec™)
• 6-place carousel pipet rack
• Bio-Rad backpack
Professional Pipet Controllers
Professional Pipet Controller, 120 V, includes charger, bench stand,
wall mount, and 0.45 µm filter
Professional Pipet Controller, 220 V, includes charger, bench stand,
wall mount, and 0.45 µm filter
Professional Pipet Controller, 220 V (for Australia), includes charger,
bench stand, wall mount, and 0.45 µm filter
Inline Filters for Pipet Controller, 0.45 µm, PTFE, 25 mm, 10 pack
Visit us on the Web at explorer.bio-rad.com
MOLECULAR BIOLOGY LABORATORY: MICROPIPETS • MICROPIPET RACKS • PIPET CONTROLLER
Micropipets
139
Molecular Biology Laboratory: Pipet Tips • Chromatography Cartridges and Systems
Chromatography Reagents and Purification Systems
Bio-Rad’s pipet tips are made from virgin polypropylene, are accurately molded
for an airtight fit, and have a smooth interior surface — essential for precise
pipetting.
• Available in bulk or in enclosed racks
• Special-purpose gel-loading and aerosol-barrier pipet tips available
• Pipet tips and racks autoclavable at a recommended temperature of 120°C
at 15 psi for 15 min
• Presterilized by 60Co gamma irradiation
Universal Pipet Tips
Available for all general pipetting applications, these tips are molded to fit
2, 10, 20, 200, or 1,000 µl micropipets.
One of the most common methods for scientific discovery is the purification of
biomolecules via column chromatography. Chromatography is a separation method
in which components of a mixture are distributed between two phases — a mobile
phase and a stationary phase. Bio-Rad offers numerous chromatography products
for the purification and characterization of proteins, nucleic acids, and peptides.
BR/TBR-14
BR/TBR-35
Prepacked columns and cartridges are the easiest and most consistent way to meet
your purification needs. Applications range from desalting oligonucleotides, antibodies,
enzymes, or protein solutions to buffer exchange or reaction cleanup. Visit us at
www.bio-rad.com/en-us/product/prepacked-columns for our complete selection
of prepacked columns and cartridges.
BR/TBR-40
Seque/Pro™ Capillary Pipet Tips
With an average diameter of less than 0.3 mm, these pipet tips are ideal for
loading sequencing gels. Use with adjustable-volume 0.1–2 µl, 0.5–10 µl, and
2–20 µl micropipets for maximum performance.
Xcluda B
Xcluda D
with the Protein Expression and
Purification Series (pp. 76–81)
High-performance, research-quality chromatography instruments
allow your students to automate, optimize, and record every step
of a chromatography procedure for a full hands-on experience in
protein purification.
Xcluda E
Prot/Elec™ Protein Gel Loading Pipet Tips
These tips are designed to slide easily between mini vertical polyacrylamide
gel plates or within cassettes with a 0.75 mm gap, yet they have a large bore for
fast sample flow. The 200 µl capacity tips are molded to fit 2–20 µl and
20–200 µl micropipets.
Train on Your
Purification Systems
Empty chromatography columns and media allow flexibility of design and
customization for the results you need. From Poly-Prep® columns to glass
Econo-Column® columns, Bio-Rad has what you need. Bio-Rad’s wide range of
chromatography media complements the empty columns allowing for a wide range
of purification options. Media choices include size exclusion, hydrophobic interaction,
anion or cation exchange, hydroxyapatite, affinity columns and more. Visit us at
www.bio-rad.com/en-us/category/chromatography for our
complete selection of empty columns and media.
Xcluda A
Xcluda™ Aerosol Barrier Pipet Tips for PCR
Xcluda aerosol-barrier pipet tips guard against aerosol contamination of samples,
a feature particularly important in PCR experiments. Available presterilized in fully
enclosed racks, they are independently tested and certified to be DNase, RNase,
and pyrogen free. Molded to fit all 2, 10, 20, 200, and 1,000 µl micropipets.
Bio-Scale™ Mini Cartridges
BioLogic™ LP System
The Biologic LP low-pressure chromatography system offers
high performance, versatility, ease of use, and affordability to
biomolecule purification. Its compact design minimizes the
workspace required in the coldroom or on the laboratory bench.
For more information on the chromatography systems visit us
online at www.bio-rad.com/en-us/category/chromatography.
Seque/Pro
Prot/Elec
Ordering Information
Tip Type, Quantity
Catalog #
Bio-Rad Micropipet Fit
Adjustable Micropipet
Fixed-Volume Micropipet
Application
List Price
EDU Price
Bulk Pipet Tips
BR-14 Tips, 1,000/bag
BR-35 Tips, 1,000/bag
BR-40 Tips, 500/bag
Prot/Elec Tips, 1,000/bag
223-9014EDU
223-9035EDU
223-9040EDU
223-9915EDU
0.1–2, 0.5–10
2–20, 20 –200
100 –1,000
0.5–10, 2–20, 20 –200
µl
µl
µl
µl
NA
5, 10, 20, 50 µl
NA
5, 10, 20 µl
General use
General use
General use
Protein gel loading
$36.00
36.00
19.00
57.00
$28.80
28.80
15.20
45.60
Racked Pipet Tips
TBR-14 Tips, 1,000/box
TBR-35 Tips, 1,000/box
TBR-40 Tips, 1,000/box
Xcluda A Tips, 960/box
Xcluda B Tips, 960/box
Xcluda D Tips, 960/box
Xcluda E Tips, 960/box
Seque/Pro Tips, 200/box
Prot/Elec Tips, 1,000/box
223-9354EDU
223-9347EDU
223-9350EDU
211-2001EDU
211-2006EDU
211-2016EDU
211-2021EDU
223-9911EDU
223-9917EDU
0.1–2, 0.5–10
2–20, 20 –200
100–1,000
0.1–2, 0.5–10
2–20
20–200
100–1,000
0.1–2, 0.5–10, 2–20
0.5–10, 2–20, 20 –200
µl
µl
µl
µl
µl
µl
µl
µl
µl
NA
5, 10, 20, 50 µl
NA
5 µl
2–20 µl
NA
NA
5 µl
5, 10, 20, 50 µl
General use
General use
General use
PCR, nucleic acids
PCR, nucleic acids
PCR, nucleic acids
PCR, nucleic acids
Sequencing gel loading
Protein gel loading
56.00
56.00
59.00
169.00
169.00
153.00
153.00
49.00
87.00
44.80
44.80
47.20
135.20
135.20
122.40
122.40
39.20
69.60
To read more about Bio-Rad’s pipet tips and packaging options, visit us on the Web at discover.bio-rad.com.
NA, not available.
BioLogic LP System and BioFrac™ Fraction Collector
For details on components included with a particular system, contact your local
sales representative or visit us on the Web at discover.bio-rad.com.
Ordering Information
Description
BioLogic LP Systems
Standard BioLogic LP System, 100/120 V, includes BioLogic LP controller, BioLogic rack, accessory kit
with inject valve (MV-6), proportioning valve/mixer, UV optics, conductivity cell, tubing and fittings kit,
column and conductivity cell holder, starter kit, and instruction manual
Standard BioLogic LP System, 220/240 V, includes BioLogic LP controller, BioLogic rack, accessory kit
with inject valve (MV-6), proportioning valve/mixer, UV optics, conductivity cell, tubing and fittings kit,
column and conductivity cell holder, starter kit, and instruction manual
BioLogic LP System with BioFrac Fraction Collector, 100/120 V, includes all standard BioLogic LP system
components, cables
BioLogic LP System with BioFrac Fraction Collector, 220/240 V, includes all standard BioLogic LP system
components, cables
BioLogic LP Data View™ Software
Catalog #
List Price
EDU Price
731-8300EDU
$9,528.00
$7,622.40
731-8301EDU
Inquire
Inquire
731-8304EDU
12,075.00
9,660.00
731-8305EDU
Inquire
Inquire
731-8365EDU
460.00
368.00
MOLECULAR BIOLOGY LABORATORY: PIPET TIPS • CHROMATOGRAPHY REAGENTS AND EQUIPMENT
Pipet Tips
To view a complete listing of Bio-Rad’s modular chromatography systems and low-pressure columns and media, visit us on the Web at discover.bio-rad.com.
140
Visit us on the Web at explorer.bio-rad.com
141
Molecular Biology Laboratory: Racks and Storage Boxes • Refresh Kit Components
Plastic Refresh Kit Components
Our plastic racks and storage units provide the ultimate in ease of storage and
sample organization.
Reduce. Reuse. Refresh. Reduce packaging waste, refresh your kits, and reuse components.
Clear and Colored Microcentrifuge Tubes
Microcentrifuge tubes provide general-purpose sample containment, manipulation, storage,
benchtop, and centrifuge use. Other features include:
Green Racks
• Durable polypropylene construction
• Capacity: 80 x 1.5/2.0 ml tubes
• Dimensions: 6.1 x 23.1 x 2.7 cm (W x D x H)
Green Racks
Storage Boxes
96-Place PCR-Tube Rack and Cover
These stackable storage units for tubes and unskirted and semi-skirted PCR plates
also provide a stable platform for preparing or centrifuging reactions.
•
•
•
•
Can be frozen or autoclaved
Fold flat for space-saving storage
Open design is ideal for use in a water bath
The top of the rack contains an embossed alphanumeric grid for easy tracking
of samples
• 15 ml rack — capacity: 60 x 15 ml tubes; dimensions: 10.5 x 25 x 7.2 cm (W x D x H)
• 50 ml rack — capacity: 24 x 50 ml tubes; dimensions: 11 x 30 x 8.5 cm (W x D x H)
Cuvette Racks
These cuvette racks are essential for organizing cuvette samples and avoiding
accidental spills. (For cuvette options see p. 131.)
Screwcap Microcentrifuge Tubes
Bio-Rad’s polypropylene screwcap micro test tubes and caps provide a high level of sample
security. O-rings are blended polyethylene/polypropylene (for solvent compatibility refer to
standard compatibility table for polyethylene). These tubes have the following features:
Gel Staining Trays
Bio-Rad’s gel staining trays are disposable plastic trays ideal for staining mini gels.
Inoculation Loops
Our sterile inoculation loops (hold 10 µl) make it easy to achieve uniform and smooth streaking without
damaging the agar surface. Using disposable inoculation loops eliminates the risk of cross-contamination
due to improper sterilization and the loops do not need flaming, making them safer to use.
15 ml Tube Rack
List Price EDU Price
50 ml Tube Rack
$71.44
108.58
90.00
90.00
$50.01
76.01
72.00
72.00
PCR Rack
96-Place PCR Tube Rack and Cover, set of 5 racks, multicolored
TRC-0501EDU
38.00
30.40
Cuvette Racks
Cuvette Racks, holds 12 standard size cuvettes, set of 5 racks
166-0485EDU
70.00
56.00
Gel Staining Trays
Inoculation Loops
Jellyfish Foam Floating Racks
Perfect for cold and hot water baths, jellyfish foam floating racks hold up to 12 microcentrifuge tubes.
Disposable Plastic Transfer Pipets (sterile and nonsterile options)
These disposable plastic transfer pipets are made of polyethylene to manage your liquid-handling needs.
166-0481EDU
166-0482EDU
166-0483EDU
166-0484EDU
Petri Dishes
96-Place PCR-Tube Rack and Cover
Ordering Information
142
500 µl
EZ Micro
Petri Dishes
Our petri dishes are ready-to-use polystyrene cell and bacterial culture petri dishes, 60 mm
diameter, sterile, and come in a package of 500 — perfect for multiple classes or experiments.
• Capacity: 12 standard size cuvettes
• Dimensions: 5 x 17.2 x 1.5 cm (W x D x H)
Catalog #
1.5 ml
Screwcap
• O-ring operating range: –55 to 150°C or caps without O-rings that are autoclavable
• Uniform walls for uniform heat transfer
• Conical bottoms for pellet formation and knurled caps for easy handling
15 ml and 50 ml Tube Racks
These economical tube racks are of durable polypropylene construction.
Plastic Racks and Storage Units
Green Rack, holds 80 tubes, 1.5/2.0 ml, set of 5 racks
Storage Box, holds 100 tubes, set of 5 boxes, multicolored
15 ml Tube Rack, holds 60 tubes, set of 5 racks
50 ml Tube Rack, holds 24 tubes, set of 5 racks
1.5 ml
EZ Micro
• Molded from polypropylene with sturdy uniform walls that easily withstand up to 13,000 x g
• Autoclavable to 120°C (clear only); freezable to –80°C (clear and colored)
• 2 ml, 1.5 ml, and 0.5 ml sizes (clear only) include marked gradations, frosted marking area,
and attached caps
• The colored microcentrifuge tubes come in six colors to easily identify reagents
Storage Boxes
• Durable polypropylene construction with a three-point hinged lid and positive latch
• Capacity: 100 x 1.5/2.0 ml tubes
• Alphanumerically labeled translucent lid provides easy sample identification
• Dimensions: 14.2 x 14.2 x 5.5 cm (W x D x H)
• Colors: green, blue, pink, orange, yellow
Description
2 ml
EZ Micro
Centrifuge and Culture Tubes
The 15 ml conical centrifuge tubes are made of polypropylene and have volume graduations
and screw caps, making these tubes perfect for general-purpose benchtop work. The sterile cell
culture tubes have round bottom snap caps and hold up to 14 ml of culture.
Jellyfish Foam Floating Racks
Disposable Plastic Transfer Pipets
Ordering Information
Description
Cuvette Rack
Catalog #
List Price
EDU Price
Plastic Refresh Kit Components
Petri Dishes, 60 mm, sterile, 500
Gel Staining Trays, 4
Inoculation Loops, 10 µl, sterile, 80
Jellyfish Foam Floating Racks, 8 racks, 12 microcentrifuge tube wells
Disposable Plastic Transfer Pipets, sterile, 1 ml, graduated, 500
Disposable Plastic Transfer Pipets, nonsterile, 1 ml, graduated, 500
166-0470EDU
166-0477EDU
166-0471EDU
166-0479EDU
166-0474EDU
166-0480EDU
$160.00
20.00
16.25
37.50
131.25
65.00
$128.00
16.00
13.00
30.00
105.00
52.00
Microcentrifuge, Centrifuge and Culture Tubes
2 ml EZ Micro™ Test Tubes, 500
1.5 ml EZ Micro Test Tubes, 500
500 µl EZ Micro Test Tubes, 1,000
1.5 ml Conical Tubes, with separate O-ring screw caps, 500
1.5 ml Conical Tubes, with installed O-ring screw caps, sterilized, 500
Colored 1.5 ml Microcentrifuge Tubes, 6 colors, 600
Conical Centrifuge Tubes, 15 ml, 50
Cell Culture Tubes, 17 x 100 mm, 14 ml, sterile, 25
223-9430EDU
223-9480EDU
223-9503EDU
224-0100EDU
224-0110EDU
166-0473EDU
166-0475EDU
166-0476EDU
31.00
29.00
43.00
88.00
123.00
38.75
32.50
21.25
24.80
23.20
34.40
70.40
98.40
31.00
26.00
17.00
1.5 ml Microcentrifuge Tubes
For more information about liquid handling products, visit us on the Web at discover.bio-rad.com and request bulletin 1981.
For information on PCR plastic supplies and accessories please see pp. 111–113.
MOLECULAR BIOLOGY LABORATORY: RACKS AND STORAGE BOXES • REFRESH KIT COMPONENTS
Racks and Storage Boxes
Conical Centrifuge Tubes
Cell Culture Tubes
Visit us on the Web at explorer.bio-rad.com
143
Ordering and General Information: Biotechnology Explorer™ Program
Bio-Rad has established a discount policy to assist the education community in the
purchase of equipment and reagents. To qualify for our educational discount, please
complete our Educational Pricing Contract Application (see p. 147) and fax it to your
local sales office. A customer care representative will call you back to set up your
personal educational account. Or simply call your local sales office and ask to speak
to a customer care representative.
How to Order From Bio-Rad Laboratories
For orders, quotes, or information, contact Bio-Rad. Customers outside the U.S.,
please refer to the back cover for your local contact information.
American Express, MasterCard, VISA, or Electronic Orders
In some countries, we may accept payment using VISA, American Express, or
MasterCard procurement cards and purchase orders. If you are interested in Bio-Rad’s
electronic ordering system, please contact the Bio-Rad sales office.
Hours
Representatives are available to take orders Monday–Friday, from 9 AM to 5 PM.
To avoid duplication of telephone orders, do not send a confirmation order unless
requested.
Special Orders and Bulk Quotations
Bio-Rad provides custom services. Many products can be supplied in bulk quantities,
for which Bio-Rad will provide special quotes on price and delivery.
Online Ordering for Select Countries
To view educational prices on our website, you will need to establish an education
account number with Bio-Rad and register online. Visit us on the Web at
explorer.bio-rad.com to register for online ordering. For further web order support,
email us at [email protected]
Purchase Agents
Bio-Rad is an ISO 9001 certified company. All Bio-Rad products are designed and
manufactured for biotechnology and life science research and must pass the most
rigorous quality control and quality assurance processes in the industry. Sole source
specifications are available for individual products upon request.
Information Needed
When Ordering
When placing orders, please provide
the following information:
• Your Bio-Rad customer account
number
• Your billing and shipping address
• Your purchase order number
• Product catalog number
• Product description
• Quantity
• Requested ship date
Product Guarantee
All Bio-Rad products are guaranteed
to meet the specifications listed in our
catalog. If you have questions about
specifications or performance, call the
Bio-Rad technical support department
at your local office.
Prices and Terms
Prices
Prices are in local currency and are subject to change without notice. Prices in effect
when your order is received will apply. Freight charges and special packaging charges
will be added to the invoice.
Call Bio-Rad for current prices if you require this information prior to placing your order.
Standard payment terms are net 30 days.
FCA Points
Hercules, California. See outside back cover for points outside the USA.
Perishable and Hazardous Materials Surcharge
Orders for products classified as dangerous goods and those requiring special
packaging with blue ice or dry ice may incur additional charges. When these fees are
applicable, Bio-Rad will advise you at the time the order is accepted.
Technical Service and Support
Within Reach Anytime, Anywhere
Whether you’re just beginning to
explore biotechnology or are an
experienced user, Bio-Rad’s technical
support specialists are available by
phone and online to provide expert
advice about products, techniques,
or applications. Call our technical
support team at your local office.
Self-service technical support can be
accessed 24 hours a day on the Web
at consult.bio-rad.com. You can
submit a question and it will be
answered by one of our specialists
within one business day. We will be
happy to help you with:
• Selecting the best product for
an application
Claims and Returns
Bio-Rad attempts to fill, check, and ship orders promptly. If errors or damage occur,
report them to Bio-Rad immediately. Report shipping damage to the carrier. Keep
containers and packing materials until an inspection is made. To return a product,
contact customer care at your local office. Bio-Rad cannot accept returns without
prior authorization.
Equipment must be accompanied by a decontamination certificate issued by the
Bio-Rad sales office. There is a nominal restocking fee on returned products. Bio-Rad
cannot authorize return of products erroneously ordered by customers if the products
require shipment on dry or blue ice. When you call, be prepared to supply your
account number, address, purchase order number, invoice number, shipping date,
product description, and catalog number.
Patent Disclaimer
Bio-Rad does not warrant that the use of products described in this catalog will not
infringe the claims of any patents covering the product itself or the use of these
products in combination with other products or in the operation of any process.
• Information on how to use
Bio-Rad products
• Replacement parts information
• Material safety data sheets (MSDS)
and other safety information
144
Visit us on the Web at explorer.bio-rad.com
ORDERING AND GENERAL INFORMATION: POLICY • PRICES AND TERMS • TECHNICAL SUPPORT
Educational Discount Policy
145
Educational Pricing Contract: Application
Ordering and General Information: Biotechnology Explorer™ Program
Literature Requests
For fast service when you need a specific piece of literature, visit us on the Web at
explorer.bio-rad.com. Here you can search our extensive literature library by type of
literature or by product. Most literature, including instruction manuals and MSDSs, is
available for download and in many cases also by mail and fax. To request literature
by phone or fax, contact your local Bio-Rad office.
Product Selection and Demonstrations
To request a demonstration or a visit from a field sales representative, contact your
local Bio-Rad office. To find the office nearest you, refer to the list on the inside back
cover of this catalog, or go to discover.bio-rad.com, click the “contact” tab, and
select your country from the drop-down menu.
Instrument Service
Bio-Rad’s commitment to customers includes continued technical support and service
after purchasing. If the instrument you purchased requires service or repair during the
warranty period, contact your local Bio-Rad office. A trained instrument service
engineer will diagnose the problem. If your instrument requires service at a Bio-Rad
facility, you will be issued a return authorization number. Upon request, an instrument
may be available for loan at no charge while your instrument is being repaired under
warranty. Bio-Rad provides on-site instrument service for certain instruments when
this is the most efficient means of providing prompt service.
Extended Warranties
Warranty extensions can be purchased for instruments including:
Econo™ components
BioLogic™ systems
Gel Doc™ systems
Gel drying systems
Thermal cyclers
Microplate readers
TC10 Cell Counter
MicroPulser™ electroporator
Model 2110 fraction collector
PowerPac™ power supplies
SmartSpec™ Plus spectrophotometer
For more information, contact your local Bio-Rad office.
Legal Notices
Notice regarding Bio-Rad thermal cyclers and real-time systems: Purchase of this instrument conveys a limited,
non-transferable immunity from suit for the purchaser’s own internal research and development and for use in
applied fields other than human in vitro diagnostics under one or more of U.S. Patents Nos. 5,656,493, 5,333,675,
5,475,610 (claims 1, 44, 158, 160–163, and 167 only), and 6,703,236 (claims 1–7 only), or corresponding claims
in their non-U.S. counterparts, owned by Applera Corporation. No right is conveyed expressly, by implication, or by
estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5' nuclease
methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing,
Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Bio-Rad’s real-time thermal
cyclers are licensed real-time thermal cyclers under Applera’s United States Patent No. 6,814,934 B1 for use in
research and for all other fields except the fields of human diagnostics and veterinary diagnostics. Purchase of iTaq™
DNA polymerase includes an immunity from suit under patents specified in the product insert to use only the amount
purchased for the purchaser’s own internal research. No other patent rights (such as 5I Nuclease Process patent
rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be
obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City,
California 94404, USA.
Bio-Rad Laboratories, Inc. is licensed by Invitrogen Corporation to sell reagents containing SYBR Green I for use in
real-time PCR for research purposes only. Purchase of this product includes an immunity from suit under patents
specified in the product insert to use only the amount purchased for the purchaser’s own internal research. No other
patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may
be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City,
California 94404, USA.
Purification and preparation of fusion proteins and affinity peptides containing at least two adjacent histidine
residues may require a license under U.S. patents 5,284,933 and 5,310,663, including foreign patents (assignee:
Hoffman–La Roche).
146
Bio-Rad Trademarks
Affi-Gel®
Aurum™
BioFrac™
Bio-Gel®
Bio-Ice™
BioLogic™
BioLogic™ LP
BioLogic DuoFlow™
Bio-Rad®
Bio-Safe™
Bio-Scale™
Bio-Spin®
Biotechnology Explorer™
C1000™
C1000 Touch™
Certified™
CFX96™
CFX96 Touch™
CFX Manager™
Chelex®
ChemiDoc™
Chill-out™
CHT™
C-Max™
Crime Scene Investigator
PCR Basics™
Criterion™
Easy Cap™
Econo™
Econo-Column®
Econo-Pac®
ELISA Immuno Explorer™
EZ Load™
EZ Micro™
Fast Blast™
GelAir™
Gel Doc™
Gene Pulser®
Genes in a Bottle™
GMO Investigator™
Got Protein?™
Image Lab™
iMark™
InstaGene™
iQ™
iScript™
iTaq™
Kaleidoscope™
LP Data View™
Macro-Prep®
Micro Bio-Spin™
Microplate Manager®
MicroPulser™
MiniOpticon™
Mini-PROTEAN®
Mini-Sub®
Mini Trans-Blot®
MJ Mini™
Molecular Imager®
Multiplate™
PCR Kleen™
pGLO™
Poly-Prep®
PowerPac™
Precision Plus Protein™
Profinity™
Prot/Elec™
Quantum Prep®
Quick Start™
ReadyAgarose™
Ready Gel®
ReadySub-Cell™
Secrets of the Rainforest™
Seque/Pro™
Silver Stain Plus™
SmartSpec™
Sub-Cell®
TC20™
TGX™
TGX Stain-Free™
trUView™
UltraRocker™
UNOsphere™
UView™
VersaFluor™
“What Causes Yogurtness”™
Xcluda™
The following trademarks are the property
of the companies listed.
Trademark
American Express
BLAST
Coomassie
DyNA Chill
Excel
Firewire
GenBank
Intel
Logger Pro
M&M’s
Mac
Mac OS
Macintosh
MasterCard
Microsoft
Mitsubishi
Pentium
Polaroid
PowerPoint
qbase PLUS
SYBR
Tween
Visa
Windows
Me, NT, XP
Owner
American Express Company
National Library of Medicine
BASF Aktiengesellschaft
Labnet International
Microsoft Corporation
Apple Computer Inc.
U.S. Department of Health
and Human Services
Intel Corporation
Vernier Software
Mars, Inc.
Apple Computer Inc.
Apple Computer Inc.
Apple Computer Inc.
MasterCard International, Inc.
Microsoft Corporation
Mitsubishi Companies
Intel Corporation
Polaroid Corporation
Microsoft Corporation
Biogazelle
Invitrogen Corporation
ICI Americas Inc.
Visa International Service
Association
Microsoft Corporation
Contract
As an educator, you are entitled to
discount pricing on all Bio-Rad
products. To establish a new education
account, simply fax or mail this page
to Bio-Rad. If you are ready to place
your first order, send this page and
attach a purchase order with the
catalog numbers and descriptions of
the items you wish to purchase.
Please contact your local Bio-Rad
office for mailing addresses and fax
numbers. See the back cover for
phone numbers.
Educational Pricing Contract Application
Re: Educational Discount
We are an educational facility interested in using Bio-Rad products to teach our
students. We would like to receive a teaching lab discount and understand that
the Bio-Rad education discount is only available to educators at the K–12 and
undergraduate levels. This letter is our confirmation that the products we order
through our educational account will be used only to educate students in a
classroom or teaching lab environment and will not be used for basic scientific
research. We are sending this letter in order to:
† Initiate setup of a new educational account with Bio-Rad Laboratories
† Place a new order, with PO attached
† Confirm an order, (please fill in order number)
Name of contact:
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EDUCATIONAL PRICING CONTRACT APPLICATION
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147
Index: Alphabetical Subject
Acrylamide, see Polyacrylamide gels
Actin . . . . . . . . . . . . . . . . . . . . . . . . . . .38–41, 123
Actin and myosin standard . . . . . . . . . .39, 41, 123
Adjustable-volume micropipets . . . . . . . . .138–139
Advanced biotechnology lab package . . . . . . . .100
Aequorea victoria . . . . . . . . . . . . . . . . . . .18, 22, 24
Affinity chromatography . . . . . . . . . . . . .76–81, 141
Agar
Biotechnology Advanced Lab Package . . .100–101
Hydrophobic interaction . . . . . . . . . . . . .20, 141
Genomic . . . . . . . . . . . . . . . . . . . . . .46, 58, 116
Evolution . . . . . . . . . . . . . . . . . . . . . . . . . . . .38, 58
Blocks, dry bath . . . . . . . . . . . . . . . . . . . . .136–137
Kits . . . . . . . . . . . .20–21, 24–25, 32–33, 78–81
Isolation kits . . . . . . . . . . . . . . . .46–47, 70, 116
Explorer Community . . . . . . . . . . . . . . . . . . . . .6–7
. . . . . . . . . . . . . . . . . . . . . . . . . . . . .18–23, 133
Blotting
Low-pressure . . . . . . . . . . . . . . . . . . . . . . . .141
Lambda . . . . . . . . . . . . . . . . . . . . . . . . . . .52–53
EZ Load DNA standards . . . . . . . . . . . . . . . . . .108
Glycine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
Buffers . . . . . . . . . . . . . . . . . . . . . . . . . .124–125
Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .141
Model kit, Classroom . . . . . . . . . . . . . . . . . .117
EZ Micro test tubes . . . . . . . . . . . . . . . . . . . . . .143
GMO Investigator kit . . . . . . . . . . . . . . . . . . .62–65
Membranes . . . . . . . . . . . . . . . . . . . . . .124–125
Size exclusion . . . . . . . . . . . . . . . . . . . . . .32–33
Molecular mass ruler . . . . . . . . . . . . . . . . . . .108
Power supplies . . . . . . . . . . . . . . . . . . . . . . .130
Supports . . . . . . . . . . . . . . . . . . . . . . . . . . . .141
Necklace kit/module . . . . . . . . . . . . . . . . .46–47
F
Grants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .161
Protein . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122
Chromosome 16 PCR . . . . . . . . . . . . . . . . . .58–59
Polymerase . . . . . . . . . . . . . . . . . . . . . .114–115
Fast Blast DNA stain . . . . . . . . . . . . . . . . . . . . .109
Green fluorescent protein, see GFP
Reagents . . . . . . . . . . . . . . . . . . . . . . . .124–125
Claims . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .145
Precut lambda . . . . . . . . . . . . . . . . . . . . . .52-53
Filter paper . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
Guarantee . . . . . . . . . . . . . . . . . . . . . . . . . . . . .145
BR-2000 vortexer . . . . . . . . . . . . . . . . . . .134–135
Classroom lab equipment packages . . . . . .90–101
Purification . . . . . . . . . . . . . . . . .46–47, 70, 116
Fingerprinting, DNA . . . . . . . . . . . . . . . . . . . . . . .50
Bradford Quickstart protein assay Got Protein?
Got Protein? Kit . . . . . . . . . . . . . . . . . . . . . . .34–35
Cloning . . . . . . . . . . . . . . . . . . . . . . . . . .24, 68–69
Quantitation . . . . . . . . . . . . . . . . . . . . . . . . . .131
Fingerprinting, fish protein . . . . . . . . . . . . . . .38–39
Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34–35
Cloning and sequencing explorer series . . . .66-–75
Restriction digest analysis . . . . . . . . . . . . .52–53
H
Fish DNA Barcoding Kit . . . . . . . . . . . . . . . . .60–61
Hardy-Weinberg equation . . . . . . . . . . . . . . .58–59
Bromophenol blue . . . . . . . . . . . . . . . . . . .107, 125
Column chromatography . . . . . . . . . . . . . . . . . . . . .
Sample loading buffer . . . . . . . . . . . . . . . . . .107
Fixed-volume micropipets . . . . . . . . . . . . .138–139
Hazardous materials shipping surcharge . . . . . .145
Buffer dam . . . . . . . . . . . . . . . . . . . . . . . . .120–121
. . . . . . . . . . .20–21, 24–25, 32–33, 78–81, 141
Sequencing (kit) . . . . . . . . . . . . . . . . . . . . .68–75
Fluorescent ruler (gel tray) . . . . . . . . . . . . . . . . . .94
HB101, E. coli . . . . . . . . . . . . . . . . . . . . . . . . . . .85
Buffer exchange . . . . . . . . . . . . . . . . . . . . . . . . .141
Combs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Size standards . . . . . . . . . . . . . . . . . . . . . . .108
Forensic DNA fingerprinting kit . . . . . . . . . . . .50–51
Helix Keepsake Vials . . . . . . . . . . . . . . . . . . .46–47
Buffers . . . . . . . . . . . . . . . . . . . . . . . .107, 124–125
Agarose gel . . . . . . . . . . . . . . . . . . . . . . . . . .105
Stains . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109
Fraction collection . . . . . . . . . . . . . . . . . . . . . . .141
Hemoglobin . . . . . . . . . . . . . . . . . . . . . . . . . .32-33
Protein gel . . . . . . . . . . . . . . . . . . . . . . . . . . .121
Tattoos, temporary, I Love Your DNA . . . . . . . .47
C
Comparative proteomics kits . . . . . . . . . . . . .38–41
DNA Electrophoresis Instructional Lab Sets . . . . .94
C1000 Touch thermal cycler . . . . . . . . . . .110–111
Competent cells . . . . . . . . . . . . . . . . . . . . .114–115
DNA Model
antibody . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
C. elegans Behavior Kit . . . . . . . . . . . . . . . . .16–17
Complete biotechnology instructional lab
Alu repeat . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58
Candy Caper, A Crime Scene Detective Kit . . . . .13
Ampicillin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
NGM Lite for C. elegans . . . . . . . . . . . . . .16–17
LB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
Agarose
Gel casting . . . . . . . . . . . . . . . . . . . . . . .85, 100
Gel drying/support film . . . . . . . . . . . . . . . . .106
Gel electrophoresis, see: Electrophoresis
Powders . . . . . . . . . . . . . . . . . . . . . . . . .85, 106
Precast gels . . . . . . . . . . . . . . . . . . . . . . . . .107
Alkaline phosphatase (AP)-conjugated
Classroom kit . . . . . . . . . . . . . . . . . . . . . . . .117
package . . . . . . . . . . . . . . . . . . . . . . . . . .98–99
Home Science kit . . . . . . . . . . . . . . . . . . . . . .13
Cartridges, see Bio-Scale Mini cartridges Cation
Coomassie, see also Stains . . . . . . . . . . . . . . . .127
dNTP mix . . . . . . . . . . . . . . . . . . . . . . . . . .114–115
Amplification reagents . . . . . . . . . . . . . . . .114–115
Exchange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .141
Conical tubes . . . . . . . . . . . . . . . . . . . . . . . .85, 143
Documentation, gel data . . . . . . . . . . . . . . . . . .132
Anion exchange . . . . . . . . . . . . . . . . . . . . . . . . .141
Cells, biological
Crime Scene Investigator PCR Basics kit . . . .56–57
Dry bath and blocks . . . . . . . . . . . . . . . . . .136–137
Criterion
DTT (dithiothreitol) . . . . . . . . . . . . . . . . . . . . . . .125
Antibiotic, ampicillin . . . . . . . . . . . . . . . . . . . . . . .85
Competent cells . . . . . . . . . . . . . . . . . .114–115
Arabinose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
Pelleting . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
Blotter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122
DyNA Chill cooler . . . . . . . . . . . . . . . . . . . .136–137
Assays
Transformation . . . . . . . . . . . . . . . . . . .18–19, 73
Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . .120–121
Drying, gels . . . . . . . . . . . . . . . . . . . . . . . .106, 121
Colorimetric detection . . . . . . . . . . . .40–41, 125
Cell counter, TC20 . . . . . . . . . . . . . . . . . . . . . . .115
Precast gels . . . . . . . . . . . . . . . . . . . . . . . . .123
Immunodetection . . . .36–37, 40–41, 42–43,125
Cell culture tubes . . . . . . . . . . . . . . . . . . . . .85, 143
Curricula . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5
Kinetic . . . . . . . . . . . . . . . . . . . .30–31, 131, 133
Cells, electrophoresis and blotting
HIC, see hydrophobic interaction chromatog
G
HindIII lambda digest . . . . . . . . . . . . . . . . . . . . . .53
GAPDH PCR module . . . . . . . . . . . . . . . . . . .70–71
Home science kits . . . . . . . . . . . . . . . . . . . . .12–13
Gel boxes
Horizontal DNA electrophoresis . . . . . . . . .104–105
Horizontal DNA . . . . . . . . . . . . . . . . . . .104–105
Horizontal electrophoresis gel . . . . . . . . . .106–107
Vertical protein . . . . . . . . . . . . . . . . . . . .120–121
Horseradish peroxidase (HRP) . . . . . . . . . . .41, 125
Gel casting, agarose . . . . . . . . . . . . . . . . .106–107
Hybridization . . . . . . . . . . . . . . . . . . . . . . . . . . .136
Gel Doc EZ system . . . . . . . . . . . . . . . . . . . . . .132
Hydrophobic interaction chromatography . .20, 141
Gel Doc XR+ system . . . . . . . . . . . . . . . . . . . . .132
Gel documentation . . . . . . . . . . . . . . . . . . . . . .132
I
Gel drying film . . . . . . . . . . . . . . . . . . . . . . . . . .106
IDEA and STEM Kits . . . . . . . . . . . . . . . . . . .48–49
Gel drying system, GelAir . . . . . . . . . . . . . . . . . .121
E
Imaging and documentation . . . . . . . . . . . . . . .132
Gel electrophoresis, see Electrophoresis
Customer service . . . . . . . . . . . . . . . . . . . .6–7, 146
Immunodetection kits . . . . . . . . . . . . . . . . . . . . .125
E. coli . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
Gel loading guides . . . . . . . . . . . . . . . . . . .120–121
iMark microplate absorbance reader . . . . . . . . .133
Protein . . . . . . . . . . . . . .34–35, 76–81, 131, 133
Criterion . . . . . . . . . . . . . . . . . . . . . . . . .120–121
Cuvette racks . . . . . . . . . . . . . . . . . . . . . . . . . . .142
EcoRI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51, 53
Gel stain . . . . . . . . . . . . . . . . . . . . . . . . . .109, 127
Criterion blotter . . . . . . . . . . . . . . . . . . . . . . .122
Cuvettes
Incubation oven, mini . . . . . . . . . . . . . . . . . . . . .137
Aurum plasmid mini purification kit . . . .69, 74, 116
EcoRl lambda digest . . . . . . . . . . . . . . . . . . . . . .53
Gel staining tray . . . . . . . . . . . . . . . . . . . . . .85, 143
Mini-PROTEAN Tetra . . . . . . . . . . . . . . .120–121
Electroporation . . . . . . . . . . . . . . . . . . .114–115
Informatics kit . . . . . . . . . . . . .58–59, 60–61, 66–75
Aurum Total RNA mini kit . . . . . . . . . . . .69, 74, 116
Educational discount . . . . . . . . . . . . . . . . . . . . .144
Gel support film . . . . . . . . . . . . . . . . . . . . . . . . .106
Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .142
Injection loop, 5 ml . . . . . . . . . . . . . . . . . . . . . . . .79
Mini-Sub cell GT . . . . . . . . . . . . . . . . . .104–105
Educational Pricing Contract Application . . . . . .147
GelAir gel drying system . . . . . . . . . . . . . . . . . .121
Spectrophotometry . . . . . . . . . . . . . . . . . . . .131
Inoculation loops . . . . . . . . . . . . . . . . . . . . .85, 143
ReadySub-Cell . . . . . . . . . . . . . . . . . . . . . . .105
Electrophoresis
Gels
InstaGene matrix . . . . . . . . . . . . . . . . . . . . . . . .116
B
Tetra blotting module . . . . . . . . . . . . . . . . . . .122
Bacteriophage lambda . . . . . . . . . . . . . . . . . .52–53
Beer’s law . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
BioFrac fraction collector . . . . . . . . . . . . . . . . . .141
Biofuel Enzyme Kit . . . . . . . . . . . . . . . . . . . . .30–31
Bioinformatics . . . . . . . . . . . .58–59, 60–61, 74–75
Bioinformatics and Sequencing Module . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . .60–61, 74–75
BioLogic chromatography system . . . . . . . . . . .141
BioLogic LP Data View software . . . . . . . . . . . .141
BioLogic LP system . . . . . . . . . . . . . . . . . . . . . .141
Bio-Rad account . . . . . . . . . . . . . . . . . . . .144, 147
Bio-Safe Coomassie protein stain . . . . . . . . . . .127
Bio-Scale Mini cartridges . . . . . . . . . . . .78–81, 141
Biotech instructional starter package . . . . . . . . . .96
Biotechnology: A Laboratory Skills Course
Textbook . . . . . . . . . . . . . . . . . . . . . . . . . .86–89
Textbook Equipment Bundles . . . . . . . . . .90–91
Textbook Kit Bundles . . . . . . . . . . . . . . . . .90–91
Biotechnology Complete Instructional Lab
Package . . . . . . . . . . . . . . . . . . . . . . . . . .98–99
148
GFP (green fluorescent protein) Chromatography Kit
1-800-4BIORAD (1-800-424-6723)
Agarose gel (DNA) . . . . . . . . . . . . . . . . .106–107
Agarose . . . . . . . . . . . . . . . . . . . . . . . . .106–107
Instructional Laboratory Equipment
Trans-blot Turbo . . . . . . . . . . . . . . . . . . . . . .122
D
Buffers . . . . . . . . . . . . . . . . . . . . . .107, 124–125
Native PAGE . . . . . . . . . . . . . . . . . . . . .124–125
Wide Mini-Sub cell GT . . . . . . . . . . . . . . . . . .105
Dangerous goods . . . . . . . . . . . . . . . . . . . . . . .145
Horizontal DNA gel boxes . . . . . . . . . . .104–105
Polyacrylamide . . . . . . . . . . . . . . . . . . .107, 123
90-91, 94-101
Cellophane sheets . . . . . . . . . . . . . . . . . . . . . . .121
Data documentation . . . . . . . . . . . . . . . . . . . . .132
Instructional packages . . . . . . . . .90–91, 94–101
Precast . . . . . . . . . . . . . . . . . . . . . . . . .107, 123
iTaq DNA polymerase . . . . . . . . . . . . . . . . .114–115
Centrifuges . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
DESTINY program . . . . . . . . . . . . . . . . . . . . . . . . .6
Mini-PROTEAN Tetra cell . . . . . . . . . . . .120–121
Protein electrophoresis . . . . . . . . . . . . .120–127
Certified agarose powders . . . . . . . . . . . . . .85, 106
Detection kits . . . . . . . . . . . . . . . . . . . . .40–41, 125
Module for Cloning and Sequencing . . . . .71, 80
ReadyAgarose . . . . . . . . . . . . . . . . . . . . . . . .107
CFX Connect Real-Time PCR detection
Digital dry bath and blocks . . . . . . . . . . . . .136–137
Pipet tips . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
SDS-PAGE . . . . . . . . . . . . . . . . . . . . . .124–125
Disposable plastic transfer pipet . . . . . . . . .85, 143
Polyacrylamide gel . . . . . . . . . . . . . . . .107, 123
TAE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107
Discount policy . . . . . . . . . . . . . . . . . . . . . . . . .144
Power supplies . . . . . . . . . . . . . . . . . . . . . . .130
TBE . . . . . . . . . . . . . . . . . . . . . . . . . . . .107, 123
Diothiothreitol (DTT) . . . . . . . . . . . . . . . . . . . . . .125
Protein . . . . . . . . . . .34–43, 76–81, 95, 120–127
TGX Precast Gels . . . . . . . . . . . . . . . . . . . . .123
DNA
Reagent packs . . . . . . . . . . . .85, 106, 126–127
Tris-HCI . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123
system . . . . . . . . . . . . . . . . . . . . . . . . . .112–113
CFX Touch Real-Time PCR detection
system . . . . . . . . . . . . . . . . . . . . . . . . . .112–113
CFX Manager software . . . . . . . . . . . . . . . .112–113
packages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
J
Jellyfish, see also Aequorea victoria . . . . . . . .18–25
Jellyfish foam floating racks . . . . . . . . . . . . .85, 143
K
Kaleidoscope standards . . . . . . . . . . . . . . .126–127
Chelex resin . . . . . . . . . . . . . . . . . . . . . . . . . . . .116
Amplification reagents . . . . . . . . . . . . . .114–115
Reagents . . . . . . . . . . . . . . . . . . . .107, 124–127
Gene transfer . . . . . . . . . . . . . . . . . . . . . . .114–115
Chemi-competent cells . . . . . . . . . . . . . . .114–115
Electrophoresis instructional lab sets . . . . . . . .94
Standards, DNA . . . . . . . . . . . . . . . . . . . . . .108
Genes in a Bottle kit . . . . . . . . . . . . . . . . . . . .46–47
Chromatography
Electrophoresis reagents . . . . . . . . . . . .106–109
Standards, protein . . . . . . . . . . . . . . . . .126–127
Genes in a Bottle Home Science Kit . . . . . . . . . .13
Affinity supports . . . . . . . . . . . . . . . . . . . . . .141
Electrophoresis stain . . . . . . . . . . . . . . . . . . .109
STEM and IDEA electrophoresis Kits . . . . .48–49
Genetic engineering . . . . . . . . . . . . . . . . .18, 24, 52
Beads . . . . . . . . . . . . . . . . . . . . . . . . . . . .32–33
Extraction kit . . . . . . . . . . . . . . . . . . .46–47, 116
Vertical . . . . . . . . . . . . . . . . . . . . . . . . .120–121
Genetically engineered protein . . . . . . . . . . . .18–21
L
Fingerprinting kit . . . . . . . . . . . . . . . . . . . .50–51
Electroporation . . . . . . . . . . . . . . . . . . . . . .114–115
Genomic DNA isolation
Laboratory notebook . . . . . . . . . . . . . . . . . . . . . .88
Fish DNA Barcoding Kit . . . . . . . . . . . . . . .60–61
ELISA Immuno Explorer kit . . . . . . . . . . . . . . .36-37
Genes in a Bottle kit . . . . . . . . . . . . . . . . .46–47
Laemmli sample buffer . . . . . . . . . . . . . . . .124–125
Fragment recovery . . . . . . . . . . . . . . . . . . . .116
Empty chromatography columns . . . . . .21, 33, 141
InstaGene matrix . . . . . . . . . . . . . . . . . . . . . .116
Lambda DNA
Gel electrophoresis . . . . . . . . . . . . . . . . . .48–63
Ethidium bromide solution . . . . . . . . . . . . . . . . .109
PV92 PCR informatics kit . . . . . . . . . . . . .58–59
Gel stain . . . . . . . . . . . . . . . . . . . . . . . . . . . .109
Eurofins MWG|Operon . . . . . . . . . . . . . . . . . . . . .75
BioLogic DuoFlow system . . . . . . . . . . . . . . .141
BioLogic LP system . . . . . . . . . . . . . . . . . . .141
Columns . . . . . . . .20–21, 24–25, 32–33, 78–81
Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . .141
Green fluorescent protein kit . . . . . . . . . . .20–21
INDEX: ALPHABETICAL SUBJECT
A
Kit refresh components . . . . . . . . . . . . . . . .85, 143
Koch’s postulates . . . . . . . . . . . . . . . . . . . . . . . .26
Precut . . . . . . . . . . . . . . . . . . . . . . . . . . . .52–53
Uncut (restriction digestion and analysis) . .52–53
Visit us on the Web at explorer.bio-rad.com
149
Index: Alphabetical Subject
Nutrient agar . . . . . . . . . . . . . . . . . . . . . . . . . .85
Nutrient broth . . . . . . . . . . . . . . . . . . . . . . . . .85
Ligation and transformation module . . . . .69, 72–73
Literature request line . . . . . . . . . . . . . . . . . . . . .146
Loading buffer, sample
Plasmid mapping . . . . . . . . . . . . . . . . . . . . . . . . .50
Native PAGE . . . . . . . . . . . . . . . . . . . . . . .124–125
Necklace kit/module . . . . . . . . . . . . . . . . . . . .46–47
Nitrocellulose membrane . . . . . . . . . . . . . . . . . .125
Nucleic acid extraction modules . . . . . . . . . . .66–70
Nucleic acid purification . . . . . . . . . . . . . . . . . . .116
DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109
Protein . . . . . . . . . . . . . . . . . . . . . . . . . .126–127
Nutrient agar . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
Nutrient broth . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
Loading guides, for protein gels . . . . . . . . .120–121
Long-wave UV lamp . . . . . . . . . . . . . . . . . . . . .133
Long-wave UV pen light . . . . . . . . . . . . . . . .19, 133
Low-pressure chromatography . . . . . . . . . . . . .141
Luer fittings . . . . . . . . . . . . . . . . . . . . . . . . . .78–79
Lysozyme . . . . . . . . . . . . . . . . . . . . . . . . . . . .21, 25
Polyacrylamide gels . . . . . . . . . . . . . . . . . .107, 123
Polymerase chain reaction, see PCR
Online resources and technical support . . .6–7, 145
Optical system software . . . . . . . . . . . . . . .112–113
Ordering . . . . . . . . . . . . . . . . . . . . . . . . .6, 144–146
Oven
Gel drying . . . . . . . . . . . . . . . . . . . . . . . . . . .121
Incubation . . . . . . . . . . . . . . . . . . . . . . . . . . .137
Micro Bio-Spin columns . . . . . . . . . . . . . . . . . . . .81
Power supplies, PowerPac . . . . . . . . . . . . . . . .130
Mini-PROTEAN TGX . . . . . . . . . . . . . . . . . . .123
Polyacrylamide . . . . . . . . . . . . . . . . . . .107, 123
ReadyAgarose, for DNA . . . . . . . . . . . . . . . .107
TGX Stain-Free . . . . . . . . . . . . . . . . . . . . . . .123
Microbes and Health Kit . . . . . . . . . . . . . . . . .26–27
Microbial culturing module . . . . . . . . . . . . . . . . . .73
Microcentrifuge . . . . . . . . . . . . . . . . . . . . . . . . .134
Micropipets
PAGE . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124–125
PBS (phosphate buffered saline) . . . . . . . . . . . .125
PCR
Amplification kits . . . . . . . . . . . . . . . . . . . .54–65
Crime Scene Investigator PCR Basics kit .56–57
Classroom . . . . . . . . . . . . . . . . . . . . . . .138–139
Disposable transfer . . . . . . . . . . . . . . . . . . . .143
Fixed-volume . . . . . . . . . . . . . . . . . . . . .138–139
Professional . . . . . . . . . . . . . . . . . . . . . .138–139
Microplate Manager software . . . . . . . . . . . . . . .133
Microplate reader . . . . . . . . . . . . . . . . . . . . . . . .133
MicroPulser electroporator . . . . . . . . . . . . .114–115
Microcentrifuge tubes or micro test tubes . . . . .142
Midi format Criterion cell . . . . . . . . . . . . . .120–121
Mini centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . .134
Mini incubation oven . . . . . . . . . . . . . . . . . . . . .137
MiniOpticon real-time PCR . . . . . . . . . . . . .112–113
Miniprep module . . . . . . . . . . . . . . . . . . . . . . . . .74
Mini-PROTEAN Tetra cell . . . . . . . . . . . . . .120–121
Mini-PROTEAN TGX precast gels . . . . . . . . . . .123
Mini ReadySub-Cell GT cell . . . . . . . . . . . .104–105
Mini Rocker . . . . . . . . . . . . . . . . . . . . . . . .134–135
Mini-Sub cell GT electrophoresis cells . . . .104–105
Mini vertical acrylamide gel electrophoresis
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .120–121
Mixing devices . . . . . . . . . . . . . . . . . . . . . .134–137
MJ Mini thermal cycler . . . . . . . . . . . . . . . .110–111
Model 16K microcentrifuge . . . . . . . . . . . . . . . .134
Molecular mass ruler . . . . . . . . . . . . . . . . . . . . .108
Molecular weight markers, see Standards
Myosin . . . . . . . . . . . . . . . . . . . . . . . . . .38–41, 123
Equipment . . . . . . . . . . . . . . . . . . . . . . .110–113
Fish DNA Barcoding Kit . . . . . . . . . . . . . . .60–61
GMO Investigator kit . . . . . . . . . . . . . . . . .62–63
Instructional Lab Package . . . . . . . . . . . . . . . .97
PV92 PCR informatics kit . . . . . . . . . . . . .58–59
Purification module . . . . . . . . . . . . . . . . .72, 116
Reagents . . . . . . . . . . . . . . . . . . . . . . . .114–116
Sample preparation . . . . . . . . . . . . . . . . . . . .116
Real-time kits . . . . . . . . . . . . . . . . . . . . . .64–65
Thermal cyclers . . . . . . . . . . . . . . . . . . .110–111
Tube adaptor . . . . . . . . . . . . . . . . . . . . . . . . .134
Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .143
PCR instructional lab package . . . . . . . . . . . . . . .97
PCR Kleen Spin Purifications Module . . . . .72, 116
Perishable Materials shipping surcharge . . . . . .145
Peristaltic pump . . . . . . . . . . . . . . . . . . . . . . . . .141
Petri dishes . . . . . . . . . . . . . . . . . . . . . . . . .85, 143
pGLO
Bacterial transformation kit . . . . . . . .18–21, 133
Lab Prep DVD . . . . . . . . . . . . . . . . . . . . . . . . .18
Plasmid . . . . . . . . . . . . . . . . . . . . . . . . . . .18–19
SDS-PAGE extension . . . . . . . . . . . . . . . .22–23
Phosphate buffered saline (PBS) . . . . . . . . . . . .125
Pipet Backpack Starter Kit . . . . . . . . . . . . . . . . .139
Pipet contoller . . . . . . . . . . . . . . . . . . . . . . . . . .139
Pipet tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
Pipets, micropipets . . . . . . . . . . . . . . . . . .138–139
Plasmid DNA . . . . . . . . . . . . . . . . . . . . . . . . . . .116
1-800-4BIORAD (1-800-424-6723)
R
Tris/borate/EDTA, see TBE
Rainforest, Secrets of the . . . . . . . . . . . .24–25, 133
Rapid Blotting — V3 Western Workflow Kit . .42–43
Reader, microplate . . . . . . . . . . . . . . . . . . . . . . .133
ReadyAgarose gels . . . . . . . . . . . . . . . . . .106–107
ReadySub-Cell GT cells . . . . . . . . . . . . . . .104–105
Reagent packs
DNA electrophoresis . . . . . . . . . . . . . . . .85, 106
Protein electrophoresis . . . . . . . . . . . . .126–127
Precut lambda DNA . . . . . . . . . . . . . . . . . . . .52–53
Real-time PCR . . . . . . . . . . . . . . . . . . . . . .112–113
Chromatography . . . . . . . . . . . . . . . . . . . . . .141
Protein
Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . .34–35
Blotting buffer and reagents . . . . . . . . .124–125
Chromatography . . . . . . . . . . . . . . . . . . . . . .141
Electrophoresis . . . .38–43, 76–81, 95, 120–125
Electrophoresis Instructional lab sets . . . . . . . .95
Restriction analysis . . . . . . . . . . . . . . . . . . . . .50–53
Returns, product . . . . . . . . . . . . . . . . . . . . . . . .145
Tris-HCI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123
Coomassie Blue . . . . . . . . . . . . . . . . . . . . . .127
trUView cuvettes . . . . . . . . . . . . . . . . . . . . . . . .131
DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109
Tube Roller . . . . . . . . . . . . . . . . . . . . . . . . .134–135
Ethidium bromide . . . . . . . . . . . . . . . . . . . . .109
Tubes
Fast Blast . . . . . . . . . . . . . . . . . . . . . . . . . . .109
Cell culture . . . . . . . . . . . . . . . . . . . . . . .85, 143
Protein . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127
Conical . . . . . . . . . . . . . . . . . . . . . . . . . .85, 143
Silver Stain Plus Kit . . . . . . . . . . . . . . . . . . . .109
Micro . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 143
Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .142
Standard gel documentation . . . . . . . . . . . . . . .132
Tween 20 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
Standards
DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .108
U
Protein . . . . . . . . . . . . . . . . . . . . . . . . . .126–127
UltraRocker rocking platform . . . . . . . . . . .136–137
STEM and IDEA Kits . . . . . . . . . . . . . . . . . . .48–49
Uncut lambda DNA . . . . . . . . . . . . . . . . . . . .53–54
Storage, boxes and racks . . . . . . . . . . . . . . . . .142
UV lamp, handheld . . . . . . . . . . . . . . . . . . . . . .133
Support media, chromatography . . . . . . . . . . . .141
UV pen light . . . . . . . . . . . . . . . . . . . . . . . . .85, 133
SYBR® Green . . . . . . . . . . . . . . . . . . . . . . .114–115
UV-transparent gel tray . . . . . . . . . . . . . . .104–105
Resins
Chelex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .116
RFLP analysis . . . . . . . . . . . . . . . . . . . . . . . .50–51
RNA isolation . . . . . . . . . . . . . . . . . . . . . . . . . . .116
Rocking platform . . . . . . . . . . . . . . . . . . . .135–136
Ruler
Fluorescent (gel tray) . . . . . . . . . . . . . . . . . . . .94
Electrophoresis standards . . . . . . . . . . .108, 126
Molecuar mass . . . . . . . . . . . . . . . . . . . . . . .108
Expression and Purification Series . . . . . . .76–81
Fingerprinting kit . . . . . . . . . . . . . . . . . . . .50–51
Tris/glycine/SDS (TGS) . . . . . . . . . . . . . . . . . . . .125
Bio-Safe Coomassie . . . . . . . . . . . . . . . . . . .127
UView Loading Dye and Stain . . . . . . . . . . . .109
Refresh kit components . . . . . . . . . . . . . . . .85, 143
Profinity IMAC . . . . . . . . . . . . . . . . . . . . . . . .76–81
Analysis kits . . . . . . . . . . . . . . . . . .30–43, 76–81
Stains
Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .142
Precision Plus Protein standards . . . . . . . .126–127
Professional development . . . . . . . . . . . . . . . . . . .4
. . . . . . . . . . . . . . . . . . . . . . . . . .90–91, 94–101
Tris/acetate/EDTA, see TAE
Spectrophotometry
Tris/glycine (TG) . . . . . . . . . . . . . . . . . . . . . . . . .125
PowerPac Mini . . . . . . . . . . . . . . . . . . . . . . . . . .130
Product guarantee . . . . . . . . . . . . . . . . . . . . . . .145
Packages, instructional laboratory equipment . . . . .
Quick Start Bradford protein assay kit . . . . . .34-35
Tris . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107, 125
Spectrophotometer, SmartSpec Plus . . . . . . . . .131
Tris buffered saline (TBS) . . . . . . . . . . . . . . . . . .125
Mapping . . . . . . . . . . . . . . . . . . . . . . . . . . . .50, 52
Membranes, blotting . . . . . . . . . . . . . . . . .124–125
Quantum Prep plasmid miniprep kit . . . . . . . . . .116
Transilluminator, Uview . . . . . . . . . . . . . . . . . . . .132
Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .142
Pricing contract . . . . . . . . . . . . . . . . . . . . . . . . .145
P
software . . . . . . . . . . . . . . . . . . .112-113
qbasePLUS . . . . . . . . . . . . . . . . . . . . . .112–113
Special packaging . . . . . . . . . . . . . . . . . . . . . . .145
PowerPac Basic . . . . . . . . . . . . . . . . . . . . . . . .130
Master Mix for PCR . . . . . . . . . . . . . . .85, 114–115
Media, chromatography support . . . . . . . . . . . .141
qbase
PLUS
Cuvettes . . . . . . . . . . . . . . . . . . . . . . . . . . . .131
Criterion . . . . . . . . . . . . . . . . . . . . . . . . . . . .123
O
Q
Poly-Prep column . . . . . . . . . . . . . .21, 25, 33, 141
Precast gels
M
150
Plasmid purification . . . . . . . . . . . . . . . . . . . . . .116
INDEX: ALPHABETICAL SUBJECT
N
LB (Luria-Bertani)
UView
T
Dye and Stain . . . . . . . . . . . . . . . . . . . . . . . .109
T100 thermal cycler . . . . . . . . . . . . . . . . . .110–111
Transilluminator . . . . . . . . . . . . . . . . . . . . . . .132
TAE (Tris/acetate/EDTA)
Electrophoresis buffer . . . . . . . . . . . . . . .85, 107
V
ReadyAgarose gels . . . . . . . . . . . . . . . . . . . .107
Vernier Software & Technology . . . . . . . . . . . . . . . .4
Tattoos, temporary, I Love Your DNA . . . . . . . . . .47
Vertical gel boxes . . . . . . . . . . . . . . . . . . . .120–121
TBE (Tris/borate/EDTA)
Vertical mini gel electrophoresis . . . . . . . . .120–121
Gel boxes . . . . . . . . . . . . . . . . . . . . . . . . . . .120
S
Gel loading . . . . . . . . . . . . . . . . . . . . . . . . . .121
Sample loading buffers . . . . . . . . . . .107, 124–125
Electrophoresis buffer . . . . . . . . . . . . . .107, 125
Green fluorescent, see GFP Purification . . . . . . .
Virus, lambda . . . . . . . . . . . . . . . . . . . . . . . . .52–53
Sample loading, protein gels . . . . . . . . . . .120–121
Mini-PROTEAN TBE precast gels . . . . . . . . .107
. . . . . . . . . . . . . . . . . . . . . . . .21, 25, 32, 76–81
Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . .32–33
Sample prep (DNA) . . . . . . . . . . . . . . . . . . . . . .116
ReadyAgarose gels . . . . . . . . . . . . . . . . . . . .107
Quantitation . . . . . . . . . . . . . . . . . . . . . . . . . .131
Vortexer, BR2000 . . . . . . . . . . . . . . . . . . . .134–135
Science Buddies program . . . . . . . . . . . . . . . . . . .4
TBS (Tris buffered saline) . . . . . . . . . . . . . . . . . .125
Stains . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127
Screwcap tubes . . . . . . . . . . . . . . . . . . . . . .85, 143
TC20 Cell Counter . . . . . . . . . . . . . . . . . . . . . . .115
Standards . . . . . . . . . . . . . . . . . . . . . . .126–127
SDS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
Teaching packages . . . . . . . . . . . . .90–91, 94–101
Protein Assessment Module . . . . . . . . . . . . . . . .78
SDS-PAGE extension . . . . . . . . . . . . . . . . . . .22–23
Technical support . . . . . . . . . . . . . . . . . . . .6–7, 145
Protein Assay Got Protein? Kit . . . . . . . . . . . .34–35
SEC, see size exclusion chromatography . . . .32–33
Temperature-controlled water bath . . . . . . . . . .137
Protein Electrophoresis Instructional Lab Set . . . .95
Secrets of the Rainforest kit . . . . . . . . . . . . . .24–25
Test tubes, micro . . . . . . . . . . . . . . . . . . . . .85, 143
Protein Expression and Purification Series . . .76–81
Sequencing and Cloning Explorer Series . . . .66–75
Tetra cell . . . . . . . . . . . . . . . . . . . . . . . . . .120–123
Protein Growth and Expression Module . . . . . . . .79
Sequencing and Bioinformatics Module . . . . .74–75
Tetra blotting module . . . . . . . . . . . . . . . . . . . . .122
Protein Profiler module . . . . . . . . . . . . . . . . . .38–39
Seque/Pro capillary pipet tips . . . . . . . . . . . . . .140
Textbook . . . . . . . . . . . . . . . . . . . . . . . . . . . .86–89
Protein SDS/PAGE Electrophoresis Module . . . . .80
Shipping . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .145
TGX precast gels . . . . . . . . . . . . . . . . . . . . . . . .123
Prot/Elec pipet tips . . . . . . . . . . . . . . . . . . . . . .140
Silver stain
Thermal cyclers . . . . . . . . . . . . . . . . . . . . .110–111
W
Warranties . . . . . . . . . . . . . . . . . . . . . . . . . . . . .146
Water bath . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137
Web resources . . . . . . . . . . . . . . . . . .6–7, 144–145
Western blot module . . . . . . . . . . . . . . . . . . .40–41
Western blotting . . . . . . . . . . . . . . .40–41, 122–125
Wide Mini ReadySub-Cell GT cell . . . . . . . . . . .105
Wide Mini-Sub cell GT cell . . . . . . . . . . . . . . . . .105
Worms, C. elegans . . . . . . . . . . . . . . . . . . . .16–17
PstI restriction enzyme . . . . . . . . . . . . . . . . . .51, 53
Documentation . . . . . . . . . . . . . . . . . . . . . . .132
Thermal printer paper . . . . . . . . . . . . . . . . . . . .132
Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .141
Silver Stain Plus kit . . . . . . . . . . . . . . . . . . . .127
Thin-wall PCR tubes . . . . . . . . . . . . . . . . . . . . .111
X
Purification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Size exclusion chromatography (SEC) . . . . . .32–33
Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
Xcluda pipet tips . . . . . . . . . . . . . . . . . . . . . . . .140
DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .116
SmartSpec Plus spectrophotometer . . . . . . . . .131
Total RNA isolation . . . . . . . . . . . . . . . . . . . . . . .116
Xylene cyanole FF . . . . . . . . . . . . . . . . . . . . . . .107
Nucleic acid . . . . . . . . . . . . . . . . . . . . . . . . .116
Software
Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . .146
Plasmid . . . . . . . . . . . . . . . . . . . . . . . . . . . . .116
BioLogic LP Data View . . . . . . . . . . . . . . . . .141
Trans-Blot Turbo . . . . . . . . . . . . . . . . . . . . . . . .122
Protein . . . . . . . . . . . . . . . . . . .21, 25, 32, 76-81
CFX Manager . . . . . . . . . . . . . . . . . . . .112–113
Transfer (blotting) membranes . . . . . . . . . .124–125
PV92 PCR informatics kit . . . . . . . . . . . . . . . .58-59
Image Lab . . . . . . . . . . . . . . . . . . . . . . . . . . .132
Transformation and Ligation Module . . . . . . .72–73
Microplate Manager . . . . . . . . . . . . . . . . . . .133
Visit us on the Web at explorer.bio-rad.com
151
Index: Catalog Number
Item Description
Page No.
145-0000
Item Description
161-0752EDU
Page No.
Catalog No.
Item Description
Gel Drying Solution, 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
165-1776EDU
Page No.
Catalog No.
Item Description
Page No.
GelAir Assembly Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
166-0012EDU
Restriction Digestion Kit TS Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . .53
145-0102EDU
TC20 Automated Cell Counter, 100/240 V . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
161-0767EDU
Sample Loading Dye, 5x, 10 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 107
165-1777EDU
GelAir Dryer, 120 V, drying oven only . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
166-0013EDU
pGLO Kit SDS-PAGE Extension . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
145-0103EDU
TC20 Automated Cell Counter with printer, 100/240 V . . . . . . . . . . . . . . . . . . .115
161-0770EDU
10x TBE, 5 L cube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107,125
165-1779EDU
GelAir Cellophane, 50 sheets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
166-0015EDU
GFP Chromatography Lyophilized Components Refill Pack . . . . . . . . . . . . .21, 25
145-0014EDU
Verification Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
161-0771EDU
10x Tris/Glycine, 5 L cube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
165-1780EDU
GelAir Drying Frame Clamps, 8 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
166-0016EDU
GFP Chromatography Buffers Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . .21, 25
145-0015EDU
TC20 Counting Slides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
161-0772EDU
10x Tris/Glycine/SDS, 5 L cube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
165-2081EDU
Gene Pulser/MicroPulser Cuvettes, 0.4 cm, 5/pk . . . . . . . . . . . . . . . . . . . . . . .115
166-0018EDU
Size Exclusion Sample Mixture and Column Chromatography Buffer . . . . . . . . .33
145-0021EDU
TC20 Trypan Blue Dye, 5 x 1.5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
161-0773EDU
50x TAE, 5 L cube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 107
165-2082EDU
Gene Pulser/MicroPulser Cuvettes, 0.2 cm, 5/pk . . . . . . . . . . . . . . . . . . . . . . .115
166-0020EDU
Bio-Gel P-60 Columns with Caps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
161-0780EDU
10x Phosphate Buffered Saline (PBS), 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
165-2083EDU
Gene Pulser/MicroPulser Cuvettes, 0.1 cm, 5/pk . . . . . . . . . . . . . . . . . . . . . . .115
166-0022EDU
Restriction Digestion Kit RT Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . .53
161-0781EDU
10% Tween 20, 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
165-2100EDU
MicroPulser Electroporator, 120/220 V, includes chamber with leads,
166-0027EDU
DNA Fingerprinting Kit Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . .51
161-0782EDU
1x TBS/1% Casein, 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
10 sterile cuvettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
166-0033EDU
pGLO Bacterial Transformation Kit curriculum manual, printed . . . . . . . . . . . . . .19
161-0783EDU
1x PBS/1% Casein, 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
165-2948EDU
Mini-PROTEAN Replacement Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
166-0037EDU
Forensic DNA Fingerprinting Kit with printed curriculum manual . . . . . . . . . . . . .50
161-0786EDU
Bio-Safe Coomassie Stain, 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127
165-3130EDU
Buffer Dams, 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
166-0043EDU
pGLO Bacterial Transformation Kit with printed curriculum manual . . . . . . . . . . .18
150-0000
150-4160EDU
Bio-Gel P-60 Gel, medium, 100 g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
156-0000
156-0080EDU
Macro-Prep HIC support,100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21, 25
161-0000
152
Catalog No.
161-0305EDU
Prestained SDS-PAGE Standards, low range, 500 µl . . . . . . . . . . . . . . . . . . . .127
161-0787EDU
Bio-Safe Coomassie Stain, 5 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127
165-3132EDU
15-Well Gel Sample Loading Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
166-0047EDU
EcoRI/PstI Restriction Enzyme Mix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51
161-0309EDU
Prestained SDS-PAGE Standards, high range, 500 µl . . . . . . . . . . . . . . . . . . .127
161-3015EDU
ReadyAgarose Precast Mini Gel, TAE, 1%, 8-well, 1 . . . . . . . . . . . . . . . . . . . .107
165-3146EDU
10-Well Gel Sample Loading Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
166-0077EDU
DNA Fingerprinting Kit curriculum manual, printed . . . . . . . . . . . . . . . . . . . . . . .51
161-0318EDU
Prestained SDS-PAGE Standards, broad range, 500 µl . . . . . . . . . . . . . . . . . .127
161-3017EDU
ReadyAgarose Precast Mini Gel, TAE, 3%, 8-well, 1 . . . . . . . . . . . . . . . . . . . .107
165-3320EDU
Gel Release, 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43, 121
166-0401EDU
Sample Loading Dye, 6x, 1 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107
161-0324EDU
Kaleidoscope Prestained Standards, 500 µl . . . . . . . . . . . . . . . . . . . . . . . . . . .127
161-3057EDU
ReadyAgarose Precast Mini Gel, TAE, 1%, 2 x 8-well, 1 . . . . . . . . . . . . . . . . . .107
165-5048EDU
PowerPac Mini Power Supply, 120/220 V . . . . . . . . . . . . . . . . . . . . . . . . . . . .130
166-0405EDU
pGLO Plasmid, lyophilized . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19, 85
161-0363EDU
Precision Plus Protein Unstained Standards, 1,000 µl . . . . . . . . . . . . . . . . . . . .127
161-3100EDU
Certified Molecular Biology Agarose, 25 g . . . . . . . . . . . . . . . . . . . . . . . . .85, 106
165-6001EDU
Criterion Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
166-0406EDU
Arabinose, lyophilized . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19, 85
161-0373EDU
Precision Plus Protein All Blue Standards, 500 µl . . . . . . . . . . . . . . . . . . . . . . .127
161-3101EDU
Certified Molecular Biology Agarose, 125 g . . . . . . . . . . . . . . . . . . . . . . . .85, 106
165-8000EDU
Mini-PROTEAN Tetra Cell, 10-well, 0.75 mm thickness; 4-gel system . . . . . . .121
166-0407EDU
Ampicillin, lyophilized . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19, 85
161-0374EDU
Precision Plus Protein Dual Color Standards, 500 µl . . . . . . . . . . . . . . . . . . . . .127
161-3102EDU
Certified Molecular Biology Agarose, 500 g . . . . . . . . . . . . . . . . . . . . . . . .85, 106
165-8001EDU
Mini-PROTEAN Tetra Cell, 10-well, 1.0 mm thickness; 4-gel system . . . . . . . .121
166-0408EDU
E. coli Strain HB101 K-12, lyophilized . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19, 85
161-0375EDU
Precision Plus Protein Kaleidoscope Standards, 500 µl . . . . . . . . . . . . . . . . . .127
161-3113EDU
Certified PCR Low-Melt Agarose, 25 g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .106
165-8002EDU
Mini-PROTEAN Tetra Cell, 10-well, 0.75 mm thickness; 2-gel system . . . . . . .121
166-0409EDU
Transformation Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19, 85
161-0404EDU
Bromophenol Blue, 10 g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107, 125
161-3114EDU
Certified PCR Low-Melt Agarose, 125 g . . . . . . . . . . . . . . . . . . . . . . . . . . . . .106
165-8003EDU
Mini-PROTEAN Tetra Cell, 10-well, 1.0 mm thickness; 2-gel system . . . . . . . .121
166-0411EDU
Bacterial Library, E. coli, lyophilized . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
161-0416EDU
10% SDS Solution, 250 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
161-3115EDU
Certified PCR Low-Melt Agarose, 500 g . . . . . . . . . . . . . . . . . . . . . . . . . . . . .106
165-8004EDU
Mini-PROTEAN Tetra Cell for Bio-Rad Precast Gels, 4-gel system . . . . . . . . . .121
166-0413EDU
HIC Chromatography Columns and Caps, 8 each . . . . . . . . . . . . . . . . . . . .21, 25
161-0423EDU
Xylene Cyanole FF, 25 g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107
161-3116EDU
Certified Molecular Biology Agarose, 5g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .106
165-8005EDU
Mini-PROTEAN Tetra Cell for Bio-Rad Precast Gels, 2-gel system . . . . . . . . . .121
166-0420EDU
Fast Blast DNA Stain, 500x, 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 109
161-0433EDU
Ethidium Bromide Solution, 10 mg/ml, 10 ml . . . . . . . . . . . . . . . . . . . . . . . . . .109
162-0000
165-8037EDU
Mini-PROTEAN Tetra Electrode Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
166-0421EDU
LB Nutrient Broth, 10 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19, 85
161-0435EDU
Coomassie Brilliant Blue R-250 Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127
162-0145EDU
0.45 µm Nitrocellulose, 7 x 8.4 cm, 10 sheets . . . . . . . . . . . . . . . . . . . . . . . . .125
165-8038EDU
Mini-PROTEAN Tetra Companion Running Module . . . . . . . . . . . . . . . . . . . . .121
166-0450EDU
Small Fast Blast DNA Electrophoresis Reagent Pack . . . . . . . .56, 62, 71, 85, 106
161-0449EDU
Silver Stain Plus Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109, 127
162-0214EDU
0.45 µm Nitrocellulose/Filter Paper Sandwich, 7 x 8.4 cm, 20 pack . . . . . . . . .125
165-8039EDU
Mini-PROTEAN Buffer Tank, replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
166-0451EDU
Small Ethidium Bromide DNA Electrophoresis Reagent Pack . . . . . . . . . . .71, 106
161-0610EDU
Dithiothreitol (DTT),1 g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
163-0000
165-8040EDU
Mini-PROTEAN Buffer Tank and Lid, replacement . . . . . . . . . . . . . . . . . . . . . .121
166-0455EDU
Medium Fast Blast DNA Electrophoresis Reagent Pack . . . . . . . . . . . .85, 71, 106
161-0611EDU
Dithiothreitol (DTT), 5 g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
163-2091EDU
165-8041EDU
Mini-PROTEAN Lid with Power Cables, replacement . . . . . . . . . . . . . . . . . . . .121
166-0456EDU
Medium Ethidium Bromide DNA Electrophoresis Reagent Pack . . . . . . . . .71, 106
161-0710EDU
2-Mercaptoethanol (BME), 25 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
164-0000
166-0460EDU
Large Fast Blast DNA Electrophoresis Reagent Pack . . . . . . . . . . . . . .71, 85, 106
161-0716EDU
Tris, 500 g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107,125
164-5050EDU
PowerPac Basic Power Supply, 120/220 V . . . . . . . . . . . . . . . . . . . . . . . . . . .130
166-0001EDU
Analysis of Precut Lambda DNA Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52
166-0461EDU
Large Ethidium Bromide DNA Electrophoresis Reagent Pack . . . . . . . . . . .71, 106
161-0717EDU
Glycine, 250 g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
164-5052EDU
PowerPac HC Power Supply, 120/220 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . .130
166-0002EDU
Restriction Digestion and Analysis of Lambda DNA Kit . . . . . . . . . . . . . . . . . . . .52
166-0462EDU
Small UView DNA Electrophoresis Reagents Module . . . . . . . . . . . . . .69, 71, 106
161-0730EDU
Urea, 250 g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
164-5056EDU
PowerPac HV Power Supply, 120/220 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . .130
166-0003EDU
pGLO Bacterial Transformation Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18
166-0470EDU
Petri Dishes, 60 mm, sterile, 500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 143
161-0732EDU
10x Tris/Glycine/SDS, 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
164-5059EDU
PowerPac HV Power Supply with Temperature Probe . . . . . . . . . . . . . . . . . . .130
166-0005EDU
Green Fluorescent Protein Chromatography Kit . . . . . . . . . . . . . . . . . . . . . . . . .20
166-0471EDU
Inoculation Loops, 10 µl, sterile, 80 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 143
161-0733EDU
10x TBE, 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107, 125
164-5070EDU
PowerPac Universal Power Supply, 120/220 V . . . . . . . . . . . . . . . . . . . . . . . .130
166-0006EDU
Secrets of the Rainforest Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24
166-0472EDU
LB Nutrient Agar Powder, 500 g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
161-0734EDU
10x Tris/Glycine, 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
165-0000
166-0007EDU
Forensic DNA Fingerprinting Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50
166-0473EDU
Colored 1.5 ml Microcentrifuge Tubes, 6 colors, 600 . . . . . . . . . . . . . . . . .85, 143
161-0737EDU
Laemmli Sample Buffer, 30 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
165-1279EDU
Gel/Blot Roller, 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122
166-0008EDU
Size Exclusion Chromatography Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32
166-0474EDU
Disposable Plastic Transfer Pipets, sterile, 500 . . . . . . . . . . . . . . . . . . . . . .85, 143
161-0738EDU
Native PAGE Sample Buffer, 30 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
165-1771EDU
GelAir Drying System, 120 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
166-0010EDU
Actin and Myosin Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
166-0475EDU
Conical Centrifuge Tubes, 15 ml, 50 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 143
161-0743EDU
50x TAE, 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 107
165-1775EDU
GelAir Drying Frames, 2 sets plus 16 clamps . . . . . . . . . . . . . . . . . . . . . . . . . .121
166-0011EDU
Precut Lambda DNA Kit Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . .53
166-0476EDU
Cell Culture Tubes, 17 x 100 mm, 14 ml, sterile, 25 . . . . . . . . . . . . . . . . . .85, 143
Sterile Water, 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65
166-0000
Visit us on the Web at explorer.bio-rad.com
INDEX: CATALOG NUMBER
Catalog No.
153
Index: Catalog Number
Item Description
166-0477EDU
Page No.
Catalog No.
Item Description
Page No.
Gel Staining Trays, 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 143
166-0530EDU
Long-Wave UV Pen Light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 133
166-0479EDU
Jellyfish Foam Floating Racks, 8 racks . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 143
166-0531EDU
UVIew mini Transilluminator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132
166-0480EDU
Disposable Plastic Transfer Pipets, nonsterile, 500 . . . . . . . . . . . . . . . . . . .85, 143
166-0540EDU
pGLO Transformation Lab Prep DVD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
166-0481EDU
Green Racks, set of 5 racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .142
166-0550BLK
166-0482EDU
Storage Boxes, set of 5 boxes, multicolored . . . . . . . . . . . . . . . . . . . . . . . . . .142
166-0483EDU
Catalog No.
Item Description
DNA Electrophoresis Instructional Lab Set 2 with upgraded
166-1039EDU
Minimal Equipment Bundle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90–91
professional digital micropipets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94
166-1042EDU
Minimal Equipment Bundle with 32 Student Editions + 1 Teacher Edition . . . . . .91
166-0705EDU
DNA Electrophoresis Instructional Lab Set 1 . . . . . . . . . . . . . . . . . . . . . . . . . . .94
166-1043EDU
Intro Biotech Course Equipment Bundle with 32 Student Editions
0.5–10 µl Classroom Digital Micropipet, set of 8 . . . . . . . . . . . . . . . . . . . . . . . .139
166-0706EDU
DNA Electrophoresis Instructional Lab Set 2 . . . . . . . . . . . . . . . . . . . . . . . . . . .94
166-0550EDU
0.5–10 µl Classroom Digital Micropipet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0709EDU
UltraRocker Rocking Platform, 120 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137
15 ml Tube Racks, holds 60 tubes, set of 5 racks . . . . . . . . . . . . . . . . . . . . . .142
166-0551BLK
2–20 µl Classroom Digital Micropipet, set of 8 . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0710EDU
Mini Rocker, 120 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
166-0484EDU
50 ml Tube Racks, holds 24 tubes, set of 5 racks . . . . . . . . . . . . . . . . . . . . . .142
166-0551EDU
2–20 µl Classroom Digital Micropipet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0711EDU
Tube Roller, 120 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
166-1051EDU
Laboratory Notebook . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88
166-0485EDU
Cuvette Racks, holds 12 standard size cuvettes, set of 5 racks . . . . . . . . . . . .142
166-0552BLK
20–200 µl Classroom Digital Micropipet, set of 8 . . . . . . . . . . . . . . . . . . . . . . .139
166-0712EDU
Mini Incubation Oven and Mini Rocker, 120 V . . . . . . . . . . . . . . . . . . . . . . . . .137
166-1052EDU
Supplementary Materials DVD Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88
166-0486EDU
Professional Pipet Backpack Starter Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0552EDU
20–200 µl Classroom Digital Micropipet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0713EDU
Mini Incubation Oven and Tube Roller, 120 V . . . . . . . . . . . . . . . . . . . . . . . . . .137
166-2000EDU
DNA Extraction Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46
166-0487EDU
Carousel Pipet Rack, holds 6 single micropipets . . . . . . . . . . . . . . . . . . . . . . .139
166-0553BLK
100–1,000 µl Classroom Digital Micropipet, set of 8 . . . . . . . . . . . . . . . . . . . . .139
166-0719EDU
UltraRocker Rocking Platform, 220 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137
166-2001EDU
Genes in a Bottle DNA Extraction Reagent Refill Pack . . . . . . . . . . . . . . . . .47, 85
166-0490EDU
Professional Pipet Controller, 120 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0553EDU
100–1,000 µl Classroom Digital Micropipet . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0720EDU
Mini Rocker, 230 V, for the UK and Europe . . . . . . . . . . . . . . . . . . . . . . . . . . .134
166-2002EDU
Lysis Buffer, 110 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47, 85
166-0491EDU
Professional Pipet Controller, 220 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0554EDU
Micropipet Rack, holds 9 single micropipets . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0721EDU
Tube Roller, 230 V, for Europe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
166-2003EDU
Protease Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58
166-0493EDU
Inline Filters for Pipet Controller, 0.45 µm, PTFE, 25 mm, 10 pack . . . . . . . . . .139
166-0555EDU
Transformation Kit Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19, 85
166-0722EDU
Tube Roller, 230 V, for the UK . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
166-2004EDU
DNA Tattoos, 200 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
166-0495EDU
Professional Micropipet, 8-channel, 20–200 µl . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0562EDU
Digital Dry Bath, 120 V, with 1.5 ml heating block . . . . . . . . . . . . . . . . . . . . . .137
166-0742EDU
50x TAE, 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107
166-2100EDU
PV92 PCR Informatics Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58
166-0499BLK
0.1–2 µl Professional Digital Micropipet, set of 8 . . . . . . . . . . . . . . . . . . . . . . . .139
166-0563EDU
Digital Dry Bath, 230 V, for the UK and Europe, with 1.5 ml heating block . . . .137
166-0755EDU
Biotech Instructional Starter Package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96
166-2119EDU
PV92 PCR Kit TS Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59
166-0499EDU
0.1–2 µl Professional Digital Micropipet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0564EDU
DyNA Chill Cooler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137
166-0756EDU
Biotech Instructional Starter Package with upgraded professional
166-2139EDU
PV92 PCR Kit RT Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59
166-0500EDU
Long-Wave UV Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 133
166-0565EDU
Digital Dry Bath Heating Block, 0.5 ml, for 24 x 0.5 ml tubes . . . . . . . . . . . . . .137
digital micropipets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96
166-2250EDU
Helix DNA Necklace Module (36 necklaces) . . . . . . . . . . . . . . . . . . . . . . . . .46, 85
166-0501EDU
Mini Incubation Oven, 120 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137
166-0566EDU
Digital Dry Bath Heating Block, 2.0 ml, for 24 x 2.0 ml tubes . . . . . . . . . . . . . .137
166-0765EDU
PCR Instructional Lab Package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97
166-2300EDU
Genes in a Bottle Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46
166-0504EDU
Temperature-Controlled Water Bath, 120 V . . . . . . . . . . . . . . . . . . . . . . . . . . .137
166-0567EDU
Digital Dry Bath Heating Block, 15 ml, for 12 x 15 ml tubes . . . . . . . . . . . . . . .137
166-0766EDU
PCR Instructional Lab Package with upgraded professional digital
166-2400EDU
ELISA Immuno Explorer Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
166-0505BLK
0.5–10 µl Professional Digital Micropipet, set of 8 . . . . . . . . . . . . . . . . . . . . . . .139
166-0570EDU
Digital Dry Bath Heating Blocks, 3 pack, includes 0.5 ml,
micropipets and C1000 Touch thermal cycler . . . . . . . . . . . . . . . . . . . . . . . . . . .97
166-2401EDU
ELISA Kit Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
166-0505EDU
0.5–10 µl Professional Digital Micropipet . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
Protein Electrophoresis Instructional Lab Set 1 with upgraded
166-2402EDU
HRP Enzyme Substrate (TMB) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
166-0506BLK
2–20 µl Professional Digital Micropipet, set of 8 . . . . . . . . . . . . . . . . . . . . . . . .139
166-0571EDU
Digital Dry Bath, 120 V, with all 4 heating blocks . . . . . . . . . . . . . . . . . . . . . . .137
professional digital micropipets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
166-2403EDU
10x PBS, 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35, 37
166-0506EDU
2–20 µl Professional Digital Micropipet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0572EDU
Digital Dry Bath, 230 V, with all 4 heating blocks . . . . . . . . . . . . . . . . . . . . . . .137
Protein Electrophoresis Instructional Lab Set 2 with upgraded
166-2404EDU
10% Tween 20, 5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
166-0507BLK
20–200 µl Professional Digital Micropipet, set of 8 . . . . . . . . . . . . . . . . . . . . . .139
166-0600EDU
LB Nutrient Agar Powder, 20 g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
professional digital micropipets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
166-2405EDU
Microplate with 12-well Strips (8 rows of 12 wells), 3 plates . . . . . . . . . . . . . . . .37
166-0507EDU
20–200 µl Professional Digital Micropipet . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0602EDU
Model 16K Microcentrifuge, 120 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
166-0825EDU
Protein Electrophoresis Instructional Lab Set 1 . . . . . . . . . . . . . . . . . . . . . . . . . .95
166-2406EDU
Antigen, chicken α-globulin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
166-0508BLK
100–1,000 µl Professional Digital Micropipet, set of 8 . . . . . . . . . . . . . . . . . . . .139
166-0603EDU
Mini Centrifuge, 120 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
166-0826EDU
Protein Electrophoresis Instructional Lab Set 2 . . . . . . . . . . . . . . . . . . . . . . . . . .95
166-2407EDU
Primary Antibody, rabbit anti-chicken polyclonal antibody . . . . . . . . . . . . . . . . . .37
166-0508EDU
100–1,000 µl Professional Digital Micropipet . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0610EDU
BR-2000 Vortexer, 120 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
166-0827EDU
Mini-PROTEAN Tetra Cell (2-gel) + Mini Trans-Blot module . . . . . . . . . . . . . . . .122
166-2408EDU
Secondary Antibody, goat anti-rabbit antibody conjugated to horseradish
166-0511BLK
5 µl Fixed-Volume Micropipet, set of 8 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0611EDU
BR-2000 Vortexer, 220 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
166-0828EDU
Mini-PROTEAN Tetra Cell (2-gel) + Mini Trans-Blot module
166-0511EDU
5 µl Fixed-Volume Micropipet, red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0612EDU
Model 16K Microcentrifuge, 220 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
166-0512BLK
10 µl Fixed-Volume Micropipet, set of 8 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0613EDU
Mini Centrifuge, 220 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
166-0512EDU
10 µl Fixed-Volume Micropipet, green . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0620EDU
166-0513BLK
20 µl Fixed-Volume Micropipet, set of 8 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0513EDU
20 ml, and 15 ml blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137
Catalog No.
Item Description
166-0701EDU
166-0810EDU
166-0820EDU
Page No.
Page No.
+ 1 Teacher Edition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
166-1044EDU
Advanced Biotech Course Equipment Bundle with 32 Student Editions
+ 1 Teacher Edition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
INDEX: CATALOG NUMBER
154
Catalog No.
peroxidase, or HRP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
+ PowerPac HC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122
166-2500EDU
GMO Investigator Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
166-1025EDU
Student Edition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88
166-2501EDU
GMO Investigator Kit Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . .63, 65
PCR Tube Adaptor, for Model 16K Microcentrifuge . . . . . . . . . . . . . . . . . . . . .134
166-1026EDU
Teacher Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88
166-2550EDU
GMO Investigator Kit Plus Small DNA Electrophoresis Reagent Pack . . . . . . . . .62
166-0621EDU
BR-2000 Vortexer, 220 V, for the UK . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
166-1027EDU
Teacher Edition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88
166-2560EDU
GMO Investigator Real-Time PCR Starter Kit . . . . . . . . . . . . . . . . . . . . . . . . . . .64
20 µl Fixed-Volume Micropipet, yellow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0622EDU
Flathead Dimpled Adaptor for BR-2000 Vortexer . . . . . . . . . . . . . . . . . . . . . . .134
166-1030EDU
Intro Biotech Course Equipment Bundle . . . . . . . . . . . . . . . . . . . . . . . . . . .90–91
166-2600EDU
Crime Scene Investigator PCR Basics Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56
166-0515BLK
50 µl Fixed-Volume Micropipet, set of 8 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0623EDU
Mini Centrifuge, 220 V, for the UK . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134
166-1033EDU
Advanced Biotech Course Equipment Bundle . . . . . . . . . . . . . . . . . . . . . . .90–91
166-2601EDU
Crime Scene Investigator PCR Basics Kit Reagent Refill Pack . . . . . . . . . . .57, 65
166-0515EDU
50 µl Fixed-Volume Micropipet, blue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139
166-0700EDU
DNA Electrophoresis Instructional Lab Set 1 with upgraded
166-1036EDU
Minimal Equipment Kit Bundle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
166-2650EDU
Crime Scene Investigator PCR Basics Kit Plus Small DNA
166-0521EDU
Mini Incubation Oven, 220 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137
professional digital micropipets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94
166-1037EDU
Intro Biotech Course Kit Bundle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
166-0524EDU
Temperature-Controlled Water Bath, 220 V . . . . . . . . . . . . . . . . . . . . . . . . . . .137
166-1038EDU
Advanced Biotech Course Kit Bundle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
Electrophoresis Reagent Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56
166-2660EDU
Crime Scene Investigator PCR Basics Real-Time PCR Starter Kit . . . . . . . . . . . .64
Visit us on the Web at explorer.bio-rad.com
155
Index: Catalog Number
Item Description
166-2700EDU
Page No.
Catalog No.
Item Description
Comparative Proteomics Kit I: Protein Profiler Module . . . . . . . . . . . . . . . . . . . .38
166-5046EDU
Hand-Packed Column Purification Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
166-2701EDU
Protein Profiler Temperature-Sensitive Reagent Refill Pack . . . . . . . . . . . . . . . . .39
166-5050EDU
Protein Expression and Purification Series, Prepacked Cartridge Process . . . . . .78
166-2800EDU
Comparative Proteomics Kit II: Western Blot Module . . . . . . . . . . . . . . . . . . . . .40
166-5051EDU
Prepacked Cartridge Purification Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
166-2801EDU
Western Blot Temperature-Sensitive Reagent Refill Pack . . . . . . . . . . . . . . . . . .41
166-5055EDU
Growth and Expression Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
166-2804EDU
Primary Antibody, anti-myosin light chain antibody, 200 µg, lyophilized . . . . . . . .41
166-5057EDU
Growth and Expression Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
166-2807EDU
0.45 µm Nitrocellulose (8 sheets)/Filter Paper Pack (16 sheets) . . . . . . . . . . . .125
166-5060EDU
SDS-PAGE Electrophoresis Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80, 127
168-0000
166-2850EDU
Comparative Proteomics Kits I and II: Protein Profiler and Western
166-5065EDU
DHFR Enzymatic Assay Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
168-1005EDU
Blot Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38, 40
166-5070EDU
Protein Expression and Purification Series, Assessment Module . . . . . . . . . . . . .78
166-2875EDU
Rapid Blotting + V3 Western Workflow Starter Kit . . . . . . . . . . . . . . . . . . . . . . . .42
166-5075EDU
166-2900EDU
Got Protein? Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
166-4000EDU
Catalog No.
Item Description
Advanced Biotechnology Lab Package with upgraded
170-4416EDU
UV-Transparent Gel Tray, 15 x 10 cm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
C1000 Touch thermal cycler (dual 48/48 block) . . . . . . . . . . . . . . . . . . . . . . . .100
170-4422EDU
Mini-Gel Caster, for Mini-Sub and wide Mini-Sub Cell trays . . . . . . . . . . . . . . . .105
166-8021EDU
Advanced Biotechnology Lab Package with Gel Doc EZ imaging system . . . . .100
170-4426EDU
UV-Transparent Gel Tray, 15 x 7 cm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
166-8022EDU
Advanced Biotechnology Lab Package with upgraded C1000 Touch
170-4434EDU
Mini-Sub Cell GT Casting Gates, for 7 x 7 cm tray only, 2 . . . . . . . . . . . . . . . .105
thermal cycler (dual 48/48 block) and Gel Doc EZ imaging system . . . . . . . . .100
170-4435EDU
UV-Transparent Gel Tray, 7 x 10 cm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
170-4436EDU
UV-Transparent Gel Tray, 7 x 7 cm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
PC Serial Cable for iMark Microplate Reader . . . . . . . . . . . . . . . . . . . . . . . . . .133
170-4446EDU
Fixed-Height Comb, 15-well, 1.5 mm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
168-1007EDU
Printer Paper for iMark Microplate Reader, 2 rolls . . . . . . . . . . . . . . . . . . . . . . .133
170-4448EDU
Fixed-Height Comb, 20-well, 1.5 mm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
IDEA Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
168-1130EDU
iMark Microplate Absorbance Reader, 100/240 V . . . . . . . . . . . . . . . . . . . . . . .133
170-4463EDU
Fixed-Height Comb, 8-well, 1.5 mm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
166-5076EDU
IDEA Kit Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
168-1135EDU
iMark Microplate Absorbance Reader with Microplate Manager Software . . . . .133
170-4465EDU
Fixed-Height Comb, 15-well, 1.5 mm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
Mini-Sub Cell GT Cell, with 7 x 10 cm tray, two 8-well combs
166-5077EDU
IDEA Kit Starter Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
168-9520EDU
Microplate Manager Software, version 6.1, PC or Mac . . . . . . . . . . . . . . . . . . .133
170-4468EDU
Wide Mini-Sub Cell GT Cell, with 15 x 10 cm tray, 15-well
(no casting gates) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
166-5080EDU
STEM Electrophoresis Teacher Demonstration Kit . . . . . . . . . . . . . . . . . . . . . . . .48
170-0000
166-4270EDU
Mini-Sub Cell GT Cell, with 7 x 7 cm tray, one 8-well comb, casting gates . . . .105
166-5085EDU
STEM Electrophoresis Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
170-2502EDU
Standard Cuvette, 3.5 ml, quartz . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .131
166-4288EDU
Mini-Sub Cell GT Cell, with 7 x 10 cm tray, two 8-well combs,
166-5090EDU
STEM Electrophoresis Classroom Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
170-2503EDU
Semimicrovolume Cuvette, 1.4 ml, quartz . . . . . . . . . . . . . . . . . . . . . . . . . . . . .131
mini-gel caster . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
166-5095EDU
STEM Electrophoresis Kit Starter Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
170-2506EDU
SmartSpec Printer Paper, 5 rolls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .131
166-5000EDU
Cloning and Sequencing Explorer Series, All Eight Modules . . . . . . . . . . . . . . . .68
166-5100EDU
Fish DNA Barcoding Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
170-2510EDU
trUView Cuvettes, 50 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .131
166-5001EDU
Curriculum Resource CD, Cloning and Sequencing Explorer Series . . . . . . . . . .69
166-5105EDU
DNA Extraction Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61
170-2511EDU
trUView Cuvettes, 100 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .131
170-6404EDU
Blotting-Grade Blocker, nonfat dry milk, 300 g . . . . . . . . . . . . . . . . . . . . . . . . .125
166-5005EDU
Nucleic Acid Extraction Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69, 70
166-5110EDU
Fish DNA Barcoding Temperature-Sensitive Refill Pack . . . . . . . . . . . . . . . . . . . .61
170-2512EDU
trUView Cuvette Height Adaptor, 15 mm . . . . . . . . . . . . . . . . . . . . . . . . . . . . .131
170-6431EDU
Horseradish Peroxidase (HRP) Conjugate Substrate Kit . . . . . . . . . . . . . . . . . . .41
166-5006EDU
Nucleic Acid Extraction Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . .70
166-5111EDU
UView 6x Loading Dye, 0.2 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109
170-2525EDU
SmartSpec Plus Spectrophotometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .131
170-6435EDU
10x Tris Buffered Saline (TBS), 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
166-5009EDU
Master Mix for PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 115
166-5112EDU
UView 6x Loading Dye, 1 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109
170-2984EDU
Gel Support Film, for drying agarose gels, 65 x 125 mm, 50 sheets . . . . . . . . .106
170-7369EDU
Computer, PC Laptop with Intel Core . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .113
166-5010EDU
GAPDH PCR Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69, 70-71
166-5115EDU
DNA Barcoding Sequencing Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
170-3342EDU
C-Max5(α) Chemi-Competent Cells, 10 x 0.05 ml . . . . . . . . . . . . . . . . . . . . . .115
170-7581EDU
Mitsubishi Thermal Printer Paper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132
166-5011EDU
GAPDH PCR Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71
166-5120EDU
C. elegans Behavior Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
170-3760EDU
Gel Cutter, 1
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
170-7950EDU
White-Light Transilluminator, for use with Gel Doc XR+ only . . . . . . . . . . . . . . .132
166-5015EDU
Ligation and Transformation Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69, 73
166-5121EDU
C. elegans Behavior Kit Reagent Room-Temperature Refill Pack . . . . . . . . . . . .17
170-3919EDU
Blue Cooling Unit for Mini-PROTEAN Tetra tanks . . . . . . . . . . . . . . . . . . . . . . .122
170-8089EDU
Mitsubishi P93DW Printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132
166-5016EDU
Ligation Module Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73
166-5122EDU
C. elegans Behavior Kit Wild-Type and Mutant Worm Strains . . . . . . . . . . . . . . .17
170-3931EDU
Mini Gel Holder Cassette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122
170-8195EDU
Gel Doc XR+ with Image Lab System for PC/Mac . . . . . . . . . . . . . . . . . . . . . .132
166-5017EDU
Transformation Module Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . .73
166-5123EDU
C. elegans Behavior Kit Temperature-Sensitive Refill Pack . . . . . . . . . . . . . . . . .17
170-3932EDU
Thick Blot Paper, 50 sheets, 7.5 x 10 cm . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
170-8198EDU
Image Lab Upgrade Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132
166-5018EDU
BglII Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73
166-5125EDU
NGM Lite Agar, 11g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
170-3933EDU
Mini Trans-Blot Fiber Pads, 8 x 11 cm, 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
170-8270EDU
Gel Doc EZ System for Mac or PC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132
166-5020EDU
Microbial Culturing Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69, 73
166-7010
Genes in a Bottle Home Science Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
170-3935EDU
Mini Trans-Blot Module, no tank . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122
170-8271EDU
UV Sample Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132
166-5021EDU
Microbial Culture Kit Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . .27, 73
166-7015
DNA Model Home Science Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
170-4070EDU
Criterion Blotter with plate electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122
170-8272EDU
White Light Sample Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132
166-5025EDU
Sequencing and Bioinformatics Module . . . . . . . . . . . . . . . . . . . . . . . . . . . .69, 75
166-7015EDU
DNA Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .117
170-4071EDU
Criterion Blotter with wire electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122
170-8273EDU
Blue Light Sample Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132
166-5026EDU
Sequencing Module Reagent Refill Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75
166-7016EDU
DNA Model Small Classroom Set, pk 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .117
170-4150EDU
Trans-Blot Turbo Transfer System for Rapid Semi-Dry Transfer . . . . . . . . . . . . .122
170-8274EDU
Stain-Free Sample Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132
166-5030EDU
Microbes and Health Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26
166-7017EDU
DNA Model Classroom Set, pk 8 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .117
170-4151EDU
Trans-Blot Turbo Cassette, single . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122
170-8276EDU
Sample Tray Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132
166-5035EDU
Biofuel Enzyme Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
166-7020
Candy Caper, A Crime Scene Detective Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
170-4152EDU
Trans-Blot Turbo Base, no cassettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122
170-8289EDU
UV/White Light Conversion Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132
166-5036EDU
Biofuel Enzyme Kit Temperature-Sensitive Reagent Refill Pack . . . . . . . . . . . . . .31
166-8005EDU
Complete Biotechnology Instructional Lab Package, 120 V . . . . . . . . . . . . . . . .98
170-4153EDU
Trans-Blot Turbo Mini PVDF 2-Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
170-8351EDU
EZ Load 20 bp Molecular Ruler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .108
166-5040EDU
Protein Expression and Purification Series, Centrifugation
166-8008EDU
Complete Biotechnology Instructional Lab Package, 120 V,
170-4154EDU
Trans-Blot Turbo Midi PVDF 2-Pack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
170-8352EDU
EZ Load 100 bp Molecular Ruler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .108
with upgraded C1000 Touch thermal cycler (dual 48/48 block) . . . . . . . . . . . . .98
170-4155EDU
Trans-Blot Turbo Starter System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122
170-8353EDU
EZ Load 100 bp PCR Molecular Ruler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .108
Advanced Biotechnology Lab Package, 120 V . . . . . . . . . . . . . . . . . . . . . . . . .100
170-4158EDU
Trans-Blot Turbo mini nitrocellulose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
170-8354EDU
EZ Load 500 bp Molecular Ruler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .108
170-4159EDU
Trans-Blot Turbo midi nitrocellulose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
170-8355EDU
EZ Load 1 kb Molecular Ruler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .108
Purification Process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .78
156
Page No.
166-5041EDU
Centrifugation Purification Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
166-5045EDU
Protein Expression and Purification Series, Hand-Packed Column Process . . . .78
166-8010EDU
Catalog No.
Item Description
166-8020EDU
Page No.
Page No.
INDEX: CATALOG NUMBER
Catalog No.
and 20-well combs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
170-4469EDU
Wide Mini-Sub Cell GT Cell, with 15 x 10 cm tray, 15-well
and 20-well combs, gel caster . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
170-4487EDU
Mini ReadySub-Cell GT Cell, for precast ReadyAgarose
gels, tank and lid only . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
Visit us on the Web at explorer.bio-rad.com
157
Index: Catalog Number
Item Description
170-8356EDU
Page No.
Catalog No.
Item Description
EZ Load Precision Molecular Mass Ruler . . . . . . . . . . . . . . . . . . . . . . . . . . . . .108
223-9750EDU
Clear Polystyrene Tubes, 13 x 100 mm, 9 ml, 1,000 . . . . . . . . . . . . . . . . . .21, 25,
500-0000
170-8870EDU
iTaq DNA Polymerase, 5 U/µl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
223-9911EDU
Seque/Pro tips, 200/box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
500-0204EDU
170-8872EDU
MgCl2 solution, 50 mM, 1.25 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
223-9915EDU
Prot/Elec Tips, 1,000/bag . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
700-0000
170-8874EDU
dNTP Mix, 200 µl premixed solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
223-9917EDU
Prot/Elec Tips, 1,000/box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
700-7253EDU
170-8880EDU
iQ SYBR® Green Supermix, 100 x 50 µl reactions . . . . . . . . . . . . . . . . . . . . . . .115
223-9950EDU
3.5 ml Standard Disposable Polystyrene Cuvettes, 100 . . . . . . . . . . . . . . . . . .131
731-0000
170-8892EDU
iScript One-Step RT-PCR Kit with SYBR® Green, 50 x 50 µl reactions . . . . . . .115
223-9955EDU
1.5 ml Standard Disposable Polystyrene Cuvettes, 100 . . . . . . . . . . . .31, 35, 131
731-1550EDU
Poly-Prep Columns, empty, 50 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21, 25, 33
170-9799EDU
Real-Time PCR Application Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65
224-0000
731-8300EDU
Standard BioLogic LP System, 100/120 V . . . . . . . . . . . . . . . . . . . . . . . . . . . .141
172-0000
172-1011EDU
Secondary Antibody, goat anti-mouse HRP, 2 ml . . . . . . . . . . . . . . . . . . . . . . . .41
184-0000
Catalog No.
Item Description
Page No.
Quick Start Bradford Protein Assay Kit 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
DEPC-Treated Water, 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70
224-0096EDU
Microplates, 96-well, EIA, polystyrene, 100 . . . . . . . . . . . . . . . . . . . . . . . .133
731-8301EDU
Standard BioLogic LP System, 220/240 V . . . . . . . . . . . . . . . . . . . . . . . . . . . .141
224-0100EDU
1.5 ml Conical Tubes, with separate O-ring screw caps, 500 . . . . . . . . . . .143
731-8304EDU
BioLogic LP System with BioFrac Fraction Collector, 100/220 V . . . . . . . . . . . .141
224-0110EDU
1.5 ml Conical Tubes, with installed O-ring screw caps,
731-8305EDU
BioLogic LP System with BioFrac Fraction Collector, 220/240 V . . . . . . . . . . . .141
sterilized, 500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 143
731-8365EDU
BioLogic LP Data View Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .141
184-0148EDU
C1000 Touch 48-Well Reaction Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .110
184-0196EDU
C1000 Touch 96-Well Reaction Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .110
345-0000
184-5096EDU
CFX96 Optical Reaction Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .113
345-0020EDU
Criterion Tris-HCl Gel, 15%, 18-well, 30 µl . . . . . . . . . . . . . . . . . . . . . . . . . . . .123
732-0113EDU
Fittings to Convert Luer to 1/4–28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
345-0033EDU
Criterion Tris-HCl Gel, 4–20%, 18-well, 30 µl . . . . . . . . . . . . . . . . . . . . . . . . . .123
732-6030EDU
InstaGene Matrix, 20 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .116
732-6100EDU
Quantum Prep Plasmid Miniprep Kit, 100 preps . . . . . . . . . . . . . . . . . . . . . . . .116
185-0000
C1000 Touch Thermal Cycler with Dual 48/48 Fast Reaction Module . . . .110
456-0000
185-1196EDU
C1000 Touch Thermal Cycler with 96-Well Fast Reaction Module . . . . . . .110
456-1023EDU
Mini-PROTEAN TGX 7.5%, 10 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . . . .123
732-6300EDU
PCR Kleen Spin Purification Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72, 116
185-5196EDU
CFX96 Touch Real-Time PCR Detection System . . . . . . . . . . . . . . . . . . . .113
456-1023SEDU U Mini-PROTEAN TGX 7.5%, 2 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . . .123
732-6400EDU
Aurum Plasmid Mini Purification Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .116
185-5200EDU
CFX Connect Real-Time PCR Detection System with reagents . . . . . . . . .113
456-1026EDU
Mini-PROTEAN TGX 7.5%, 10 Gels/Box, 15-well, 15 µl . . . . . . . . . . . . . . . . .123
732-6470EDU
Aurum Vacuum Manifold . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .116
185-5201EDU
CFX Connect Real-Time PCR Detection System . . . . . . . . . . . . . . . . . . . .113
456-1033EDU
Mini-PROTEAN TGX 10%, 10 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . . . .123
732-6820EDU
Aurum Total RNA Mini Kit, 50 preps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .116
456-1033SEDU U Mini-PROTEAN TGX 10%, 2 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . . .123
732-6826EDU
Aurum Mini Columns, 50 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70
456-1036EDU
Mini-PROTEAN TGX 10%, 10 Gels/Box, 15-well, 15 µl . . . . . . . . . . . . . . . . .123
738-0000
456-1043EDU
Mini-PROTEAN TGX 12%, 10 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . . . .123
738-0014EDU
186-1096EDU
T100 Thermal Cycler, 120/240 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .110
211-0000
Domed 12-Cap Strips, for 0.2 ml tubes and plates, clear, 200 . . . . . . . . . . . . .111
TFI-0201EDU
0.2 ml Tubes with Flat Caps, clear, 1,000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .111
TLS-0851EDU
Low profile 0.2 ml 8-tube Strips without Caps, white, 120 . . . . . . . . . . . . . . . .113
TRC-0501EDU
96-Place PCR Tube Rack and Cover, 5 . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 142
TWI-0201EDU
0.2 ml Tubes with Domed Caps, clear, 1,000 . . . . . . . . . . . . . . . . . . .85,111, 113
Flow Adaptor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
211-2001EDU
Xcluda A Tips, 960/box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
456-1043SEDU U Mini-PROTEAN TGX 12%, 2 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . . .123
750-0000
211-2006EDU
Xcluda B Tips, 960/box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
456-1046EDU
Mini-PROTEAN TGX 12%, 10 Gels/Box, 15-well, 15 µl . . . . . . . . . . . . . . . . .123
750-0497EDU
211-2016EDU
Xcluda D Tips, 960/box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
456-1083EDU
Mini-PROTEAN TGX 4–15%, 10 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . .123
760-0000
211-2021EDU
Xcluda E Tips, 960/box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
456-1083SEDU U Mini-PROTEAN TGX 4–15%, 2 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . .123
760-0037EDU
BioLogic DuoFlow System, 100/120 V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
456-1086EDU
Mini-PROTEAN TGX 4–15%, 10 Gels/Box, 15-well, 15 µl . . . . . . . . . . . . . . .123
760-0047EDU
BioLogic DuoFlow System with BioFrac Fraction Collector . . . . . . . . . . . . . . . . .79
Mini-PROTEAN TGX 4–20%, 10 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . .123
CHO-1411EDU
Chill-out Liquid Wax, clear, optical grade, 100 ml . . . . . . . . . . . . . . . . . . . . . . .115
223-0000
TCS-1201EDU
732-0000
185-1148EDU
186-0000
158
Page No.
INDEX: CATALOG NUMBER
Catalog No.
5 ml Injection Loop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
223-9014EDU
BR-14 Tips, 1,000/bag . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
456-1093EDU
223-9035EDU
BR-35 Tips, 1,000/bag . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
456-1093SEDU U Mini-PROTEAN TGX 4–20%, 2 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . .123
ECT-1000EDU
Easy Cap Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .111
223-9040EDU
BR-40 Tips, 1,000/bag . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
456-1096EDU
Mini-PROTEAN TGX 4–20%, 10 Gels/Box, 15-well, 15 µl . . . . . . . . . . . . . . .123
ECT-2000EDU
PCR Strip Cap Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .111
223-9347EDU
TBR-35 Tips, 1,000/box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
456-9033EDU
Mini-PROTEAN TGX, Any kD, 10 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . .123
MLL-4851EDU
Multiplate Low Profile 48-well Unskirted PCR plates, white, 50 . . . . . . . . .111, 113
223-9350EDU
TBR-40 Tips, 1,000/box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
456-9033SEDU Mini-PROTEAN TGX, Any kD, 2 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . . .123
MLL-9651EDU
Multiplate Low-Profile 96-well Unskirted PCR Plates, white, 25 . . . . . . . . . . . .113
223-9354EDU
TBR-14 Tips, 1,000/box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
456-5013EDU
Mini-PROTEAN TBE 5%, 2 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . . . . . .123
MLP-9601EDU
Multiplate 96-well Unskirted PCR Plates, clear, 25 . . . . . . . . . . . . . . . . . . . . . .111
223-9430EDU
2 ml EZ Micro Test Tubes, 500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85,143
456-5033EDU
Mini-PROTEAN TBE 10%, 2 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . . . . .123
TBS-0201EDU
0.2 ml 8-Tube Strips without Caps, clear, 125 . . . . . . . . . . . . . . . . . . . . . . . . .111
223-9480EDU
1.5 ml EZ Micro Test Tubes, 500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 143
456-5053EDU
Mini-PROTEAN TBE 15%, 2 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . . . . .123
TBS-1201EDU
0.2 ml 12-Tube Strips without Caps, clear, 100 . . . . . . . . . . . . . . . . . . . . . . . .111
223-9500EDU
PCR Tube Capless Adapters, 500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85, 111
456-5093EDU
Mini-PROTEAN TBE 4–20%, 2 Gels/Box, 10-well, 30 µl . . . . . . . . . . . . . . . .123
TCS-0801EDU
Domed 8-Cap Strips, for 0.2 ml tubes and plates, clear, 120 . . . . . . . . . . . . . .111
223-9503EDU
500 µl EZ Micro Test Tubes, 1,000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .143
456-8093EDU
Mini-PROTEAN TGX 4-20%, Stain-Free gels (10) . . . . . . . . . . . . . . . . . . . . . .123
TCS-0803EDU
Optical Flat 8-Cap Strips, for 0.2 ml tubes and plates ultraclear, 120 . . . . . . . .113
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159
Biotechnology Explorer™ Program: Meet the Explorers
Renee
LeMaireAdkins, PhD
Biotechnology
Explorer
Senior Manager
Sherri
Andrews, PhD
Biotechnology
Explorer
Curriculum
Training Specialist
Erika
Fong
Biotechnology
Explorer
Sales Manager
Jose
Lopez, PhD
Biotechnology
Explorer
Scientist
Leigh
Brown
Biotechnology
Explorer
Curriculum
Training Specialist
Nicole
Hamilton
Biotechnology
Explorer
Product Manager
Ingrid
Miller, PhD
Biotechnology
Explorer
Marketing
Manager
Jeannie
Spagnolo, PhD
Biotechnology
Explorer
Staff Scientist
Bryony
Ruegg, PhD
Biotechnology
Explorer
Manager
Teaching Awards and
Program Funding — Realize
your teaching dreams with
awards and grants!
Bio-Rad sponsors two awards
for teaching excellence and a
competitive grant for $2,000
in Bio-Rad materials. Teaching
awards and grants are a great
way to realize your dreams
to engage and excite your
students! The Ron Mardigian
Biotechnology Explorer awards
were established in memory of
Ron Mardigian, the inspirational
guru and founder of the
education program.
The Ron Mardigian Memorial Biotechnology Explorer Award
is a partnered award with the National Science Teachers Association (NSTA).
Deadline November 30, awarded annually. (www.nsta.org)
2013 NSTA Ron Mardigian Memorial
Biotechnology Explorer Award Winner
Susan Mumford-Hartley
Hinkley High School, Aurora, CO
The Ron Mardigian Biotechnology Teaching Award
is also a partnered award, this time with the National Association of Biology
Teachers (NABT). Deadline March 15, awarded annually. (www.nabt.org)
Damon
Tighe
Biotechnology
Explorer Curriculum
Training Specialist
BIOTECHNOLOGY EXPLORER PROGRAM: MEET THE EXPLORERS
Team Members
2012 NABT Ron Mardigian
Biotechnology Award Winner
Peggy Deichstetter
St. Edward High School, Elgin, IL
Bio-Rad Biotechnology Explorer Mini-Grant
To motivate you to put your dreams on paper and gain valuable experience,
we sponsor a $2,000 grant for Bio-Rad materials. Applications are due
annually on February 1.
Associate Members
Kirk Brown
Director, Science and STEM
Integration and Innovation,
San Joaquin County Office of
Education and Biotechnology
Explorer Professional
Development Team
Cindy Gay
Science Teacher,
Steamboat Springs
High School, Colorado
and Biotechnology
Explorer Professional
Development Team
160
Kristi DeCourcy, PhD
Lab Manager, Fralin
Biotechnology Center,
Virginia Tech and
Biotechnology
Explorer Professional
Development Team
Please visit us at bio-rad.com/explorer_community_funding
Stan Hitomi
Principal, Alamo School,
Math/Science Coordinator,
San Ramon Valley
Unified School District
and Biotechnology
Explorer Professional
Development Team
2013 Bio-Rad Biotechnology Explorer
Mini Grant Winner
Patti Erikson
Salisbury University, Salisbury, MD
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161

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