User Manual SuperCycler

User Manual SuperCycler
User Manual
SuperCycler
SC-200
Revision 3.0
2011
Korea
Kyratec Life Science
IT Premier Tower 1304, 345-50
Gasan-dong Geumcheon gu
Seoul Korea, 153-803
Website: www.kyratec.com
Australia
Kyratec Pty Ltd
3/17 Dividend St Mansfield
Brisbane, Queensland
Australia, 4122
Website: www.kyratec.com
Contents
1. Foreword .................................................................................................................................................................. 3
2. Features.................................................................................................................................................................... 4
2.1 General .......................................................................................................................................................... 4
2.2 Configuration ................................................................................................................................................. 4
2.3 Linear Gradient (*available in selected regions only) .................................................................................... 4
2.4 Thermal Profile Engine................................................................................................................................... 4
2.5 Long Range .................................................................................................................................................... 4
2.6 Touch Down ................................................................................................................................................... 4
2.7 Pause.............................................................................................................................................................. 4
2.8 Manual Control .............................................................................................................................................. 5
2.9 Evaporation control Heated Lid ..................................................................................................................... 5
2.10 User Interface .............................................................................................................................................. 5
3. Getting Started ......................................................................................................................................................... 6
3.1 Region Selection ............................................................................................................................................ 6
3.2 Navigating the Software ................................................................................................................................ 7
4. PCR Profile Creation ................................................................................................................................................. 8
4.1 Wizard Mode ................................................................................................................................................. 8
5. Manual Profile Generation and Execution ............................................................................................................. 10
5.1 Creating a new Event ................................................................................................................................... 11
5.2 Starting a run ............................................................................................................................................... 12
6. Manual Block Control............................................................................................................................................. 14
7. Configuration Tools ................................................................................................................................................ 15
7.1 User Accounts .............................................................................................................................................. 16
7.2 File Manager................................................................................................................................................ 16
7.3 Machine Settings ......................................................................................................................................... 17
7.4 Software Update .......................................................................................................................................... 18
7.5 Games & Extras............................................................................................................................................ 18
8. User Tips................................................................................................................................................................. 19
9. Specifications ......................................................................................................................................................... 20
10. Terms of Use......................................................................................................................................................... 21
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1. Foreword
Congratulations on purchasing a Kyratec SuperCycler. You are now one of a growing number of highly skilled
customers worldwide to adopt the SuperCycler as their preferred Thermal Cycling Engine.
Our philosophy has been to generate user friendly yet powerful cycling workhorse. We trust you will be most
pleased with our product however always welcome any suggestions you may have to make our products better for
the future. Feel free to email us: [email protected]
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2. Features
2.1 General
The SuperCycler is a high performance block cycling system configured and optimised for Industry standard 200ul
individual or strip tubes (domed or flat-capped) or 96-well plates (low or high skirt) with strip caps or adhesive film
seals. It incorporates state of the art electronics, precision quality Ferrotec™ peltier devices and a flexible user
interface.
2.2 Configuration
The SuperCycler utilises eight Peltier devices to actively heat and cool the block between 4°C and 99°C. These are
arranged into four zones with independent sensing and control capabilities. This Configuration of peltiers and
sensors enables a highly linear gradient to be established via a sophisticated computer control system.
2.3 Linear Gradient (*available in selected regions only)
Thermal gradient technology enables a varying temperature to be set across the reaction wells of the block.
Applications of this feature include the ability to optimize the annealing temperature of an assay in a single
experiment by determining the temperature of the wells that yield the best result. The SuperCycler is capable of
generating a linear gradient of between 0°C and 20°C across the block. The software interpolates and displays the
temperature of each column of wells in real-time during a run.
2.4 Thermal Profile Engine
The SuperCycler has a powerful thermal profile engine implemented within. A profile may contain up to 100
events. Each ‘event’ can be either a hold at temperature, pause or 2 to 5 step cycling with up to 100 repeats. Any
event or step can contain gradient, touchdown or long range features. An almost unlimited number of profiles may
be stored on the device for re-running. Despite its capabilities profile setup is straightforward.
2.5 Long Range
The Long Range feature enables the time of a particular cycling step to be automatically increased or decreased by
a preset amount over a specified range of cycle repeats. The Long Range increasing time mode feature is often
used to provide gradually extended times for enzymatic polymerization of longer products during later cycle
repeats of an amplification reaction. It is most useful for targets longer than about 1kb. Long Range decreasing
time mode can be used to shorten the total run time. During later cycles the majority of enzymatic polymerization
is occurring on previously generated amplicons which are shorter in length than the original template. Shorter
hold times are often sufficient for polymerization of these shorter length products.
2.6 Touch Down
The Touchdown function enables the temperature of a step to be automatically decreased by a preset amount
over a range of successive cycle repeats. Its primary use is as a mechanism to minimise primerdimer artifacts by
gradually decreasing the annealing temperature during initial cycle repeats of an amplification profile.
2.7 Pause
The Pause function allows the user to pause the profile at any number of pre programmed points with an audible
beep. The current set temperature is held indefinitely until the Continue button is pressed. Holds are useful if
there is a need to remove tubes or add reagents at a particular point in a run.
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2.8 Manual Control
The SuperCycler also supports Manual Mode control. This mode enables the user to set the block to a specific
temperature quickly without creating a thermal profile. This function is useful for incubating reactions such as DNA
digestion or ligation. Manual Mode also supports thermal gradient.
2.9 Evaporation control Heated Lid
The SuperCycler employs a heated lid design to keep the air contained within the tube hotter than the reaction
volume. This causes any evaporation to condense back into the cooler reaction liquid thereby eliminating the need
for oil or wax condensation overlay.
2.10 User Interface
The SuperCycler has a 7” colour touch screen giving a vibrant and flexible means of run setup and monitoring. This
internal graphical user interface controller provides a powerful yet intuitive and easy to use interface. All
SuperCyclers have a USB port and (soon to be released) Windows based PC software. This software opens the user
up to easier profile setup, sharing of files, and post run examination of any temperature data.
New software features and updates are regularly incorporated and available for either system as free upgrades via
web download.
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3. Getting Started
Plug the unit into a power outlet and power the unit on with the switch located on the rear of the unit. Do not
worry about selecting the correct power voltage for your country, The SuperCycler is designed to operate in any
country in the world with no changes. The unit is also designed to be resistant to power surges and drop-outs that
occur in countries with less reliable power grids.
On power up the SuperCycler will display the logo and take a few moments to initiate the software.
3.1 Region Selection
When the unit is powered on for first time ever the region selection and End User Licence Agreement (EULA) will
be displayed. The operator is required to select their correct region of purchase and operation, read the terms of
use and check the 'agree' box then press the green tick to continue. Note that available features vary between
different regions. The features that are available for your use are always as specified to you before your purchase
of the equipment.
If the instrument is moved to a different region of operation at a later date then the region configuration screen
may be reached through the 'settings' icon under the configuration menu.
A copy of the Terms of use may also be found at the end of this manual.
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3.2 Navigating the Software
After power up the unit defaults to the main screen from where all features may be accessed. You may return to
this screen at any time without interrupting a run by pressing the Home logo in the bottom left hand corner for
the screen.
The 4 screen icons provide access the following features:
CONFIGURATION – Takes you to the configuration page allowing of machine options.
PCR RUN – Is used to load or create a new PCR thermal profile and initiate its execution.
MANUAL CONTROL – Allows immediate manual setting of block temperature.
INFORMATION – Displays a general information screen which includes such information as serial number, software
and hardware version numbers. An on-screen user manual is accessible from under this page menu
The bar at the bottom of the screen displays useful information about the device and run status. The first box
displays the current activity; it displays such information as 'idle', 'ramping to temperature' and 'hold – xx' with a
countdown of the remaining hold time at the particular step. The second box displays the Elapsed run time and
the last box the total Estimated run time. The actual run time may slightly differ from the estimated time
depending on factors such as the ambient air temperature, humidity and the type of plastic ware and liquid
volumes on the block. The current user name and profile picture is also displayed as well as the current time and
date.
Throughout the software help is available in numerous locations by pressing the question mark logo if available.
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4. PCR Profile Creation
From the 'home' screen the 'PCR Run' icon will bring you to the experiment page. Here you will have 4 options,
and you can load an existing experiment from either the internal drive or an external USB drive, or create a new
thermal profile using either the Wizard or the manual builder.
When loading files from the 'Internal Drive' you only have access to the files under the directory of the user that
you are currently logged in as. If you are not logged in as a registered user your file access will be under the
'Default' user directory.
4.1 Wizard Mode
The SuperCycler software includes a Wizard utility which enables the user to configure easy to moderate
complexity profiles in just moments. To activate select 'Wizard' from the PCR start-up screen.
The Wizard screen displays each of the steps which occur in a typical range of profiles. At a minimum there are 3
steps, Preheat and then denaturation and anneal which are cycled. You may enable up to a further 3 steps (cDNA,
Extension & post run hold) by clicking on the corresponding check boxes. Each of the steps will be executed in the
order they are shown arranged down the screen with the Denaturation, Anneal and Extension (if enabled) steps
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being cycled. After the amplification run has been completed the temperature may be held at a defined
temperature until the user disables the unit. To adjust any of the parameters click on the corresponding time or
temperature boxes and a keypad will be presented allowing entering of new numbers. The number of cycles can
also be adjusted between 1 (single execution, no loops) and 99 cycles.
When the user is happy with the profile parameters just click on the tick. A run review screen will now be shown
with a text description of the profile steps. Click on OK to commence run execution or 'Edit Profile' if you require to
further modify the wizard created profile. The 'Save & Run' Icon allows you to save the profile then run it.
Once the profile is confirmed, if the 'Use Top Heater' checkbox option is selected (default) then you will be
prompted with a screen asking if you wish to wait for the top heater to reach target temperature before
commencing the run. If you select to wait for the top heater to achieve temperature a waiting screen will be
displayed. You may skip the waiting step at any time if you wish to commence the run immediately. Depending on
the set temperature of the Top Heater it will take up to several minutes to pre heat to this temperature.
When the run commences the software will automatically proceed to the 'Run' screen. You are not able to modify
the profile once a run has commenced but you may save or edit it after it completes or is terminated.
From the run review screen you may also check the option 'run in virtual mode'. This will make the SC simulate the
run rather than performing the actual thermal steps on the block. This can be useful if you wish to check
complicated runs prior to performing them.
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5. Manual Profile Generation and Execution
The manual profile editor is evoked from the PCR Experiment screen. This screen displays the current experiment
profile in a 'tree list' format. When you select an event from the tree list, the corresponding thermal profile for
that event will be displayed on the right hand side of the screen. Note that it is only the first few minutes of that
event which are displayed graphically. When an event is selected a sub menu will be displayed giving further
options for this event. The selected event may be moved up or down in order within the profile events, deleted, or
edited.
The profile generation screen contains 8 icon buttons with the following functionality:
HELP – This button will display the on screen user manual with the relevant pages selected
START RUN – This button is used to activate a run. Pressing this button will show a profile review before
commencing a run
ADD EVENT – Evokes the Add Step wizard to build a new profile step.
ADD NOTES – Allows the operator to write and save any notes along with the run.
SAVE – Allows a given profile to be saved to internal flash memory disk. The unit has very large internal memory
allowing virtually unlimited profiles to be saved (in the order of tens of thousands). Saving a profile also stores the
device options configurable under the settings menu, i.e. top heater temperature and other functionality. You may
enter any valid file name for saving. Profile file will be saved to the current user directory or the Default directory if
no user is selected.
LOAD – Loads a pre-saved profile from the internal flash memory. The SuperCycler displays a list of the saved files
on the unit in the directory of the currently selected user. If no user is selected it will show the files saved in the
default user directory. To access a different user's files you need to log in as that user.
CLEAR PROFILE – will erase the current profile. User confirmation is requested before execution.
SETTINGS – will display the system settings allowing changes to top heater function and temperature and other
functions.
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5.1 Creating a new Event
To create a new event select the 'Add Event' icon in the manual profile editor. The first screen of the Add Event
Wizard which will be displayed lets the user select the type of event.
You may choose from 4 event types.
HOLD – Is a simple hold at given temperature for a set time. It may be performed either with or without a gradient
across the block.
The hold screen allows the operator to set the hold time and temperature. If the Gradient* option is selected both
the left and right temperatures may be set separately and the predicted well temperatures can be shown by
clicking the Predicted Temps link.
RAMP – Allows the block temperature to be ramped either up or down between two set temperatures over a
preset length of time. The block target temperature is updated every second and with a 0.025 degree minimum
step resolution. It may be performed either with or without a gradient across the block.
The ramp screen shows the initial and final ramp temperatures. If the Gradient* option is selected both the left
and right temperatures may be set separately and the predicted well temperatures can be shown by clicking the
Predicted Temps link.
PAUSE – will hold the temperature at the last set point and pause the run with an audible warning. This is useful if
the operator needs to perform a certain action of the samples at a given point in the run. The operator clicks ok
when complete and the run will continue with the next event.
CYCLING x STEP – will proceed to generate a multi step cycling event. The scroll button to the right allows you to
change the number of steps between 2 and 5 for the cycling event.
Within the cycling configuration screens there are numerous options. Once again If the Gradient* option is
selected both the left and right temperatures may be set separately and the predicted well temperatures can be
shown by clicking the Predicted Temps link. There are also options for Touchdown and Long range features.
Selecting these checkboxes will display extra buttons to configure these features.
Touch Down – allows for the temperature of a given step within a cycling event to be progressively increased or
decreased from an initial value to a final value over a defined number of steps.
Long Range – allows the hold time of a given step within a cycling event to be progressively increased or
decreased from an initial value to a final value over a defined number of steps.
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Any or all of the steps within a cycling event may use these features. The following picture displays the same
screen with all options (Gradient*, Touch Down & Long Range) enabled.
The 'Next' button will proceed through each of the parameter screens for each step contained within the cycling.
After the parameters for each step are configured the operator is prompted to enter the number of times to repeat
the cycle.
*Note - Gradient function not available in all regions or in all models.
5.2 Starting a run
Activating the Start Run button will show a profile review page which allows the operator to check over the profile
(displayed in text format) before confirming commencement. The profile review will also contain general run
options regarding the Top Heater evaporation control and actions on run completion. These are configurable
under the configuration menu. You have the option to perform the run in virtual mode; this will simulate the run
without actually thermally varying the block.
Once the profile is confirmed, If the 'Use Top Heater' checkbox option is selected (default) under the setup menu
then you will be prompted with a screen asking if you wish to wait for the top heater to reach target temperature
before commencing the run.
If you select to wait for the top heater to achieve temperature a waiting screen will be displayed. You may skip the
waiting step at any time if you wish to commence the run immediately. Depending on the set temperature of the
Top Heater it will take up to several minutes to pre heat to this temperature.
Once the run commences the software will automatically proceed to the 'Run' screen. You are not able to modify
the profile once a run has commenced.
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On run completion the post run report screen will show and if enabled an audible alarm will sound. The alarm may
be muted by pressing the mute alarm button. The post run report can be saved as a text file to a USB memory stick
for inclusion into lab books etc. Note that post run reports are not saved to internal memory.
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6. Manual Block Control
The manual mode screen allows immediate manual setting of the block temperature. To activate this mode simply
press the blue 'start' button. Terminating the mode is achieved by pressing the red 'stop' button. To Program the
set temperature press the temperature button to the left of the graph and enter the desired value. You may
program the temperature set point to 0.1 degree C resolution. If the 'Gradient'* checkbox is checked the 'Temp
Right' button appears and you are able to set different temperatures for both the left and right sides of the block
with a roughly linear thermal gradient presented between the two outer well sides. To enable the Top Heater
evaporation control click the 'Top Heat' checkbox. The target temperature of the top heater is configured under
the Configuration → Sengs menu. Note the top heater will take a few minutes to reach its operang
temperature. The left side of the screen displays the temperatures of each of the 4 zones. Other information also
displayed is the event, step and cycle numbers for when a profile is being executed. At the bottom of this display
box is the top heater (TH) and internal heat sink (HS) temperatures.
*Note gradient feature not available all regions
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7. Configuration Tools
The configuration page provides access to the following features.
FILE MANAGER – For managing the PCR run files on the device and copying from the device using a USB memory
stick.
MACHINE SETTINGS – Allows changes to be made to the instruments settings.
TIME & DATE – Used to set the Time for the instruments internal clock.
THERMAL CALIBRATION – The thermal calibration feature is used to adjust the machine calibration. SuperCyclers
are factory calibrated and should not require user recalibration for the life of the machine. The recalibration
procedure is not covered in this user manual. If it is required, contact the distributor the equipment was purchased
through for 'SC Service note – Thermal recalibration'. The thermal calibration is password protected to prevent it
accidentally being changed.
USER ACCOUNTS – User account control.
SOFTWARE UPDATE – Allows upgrading of the SuperCycler operating Software.
TOUCH PANEL CALIBRATION – You can also perform a calibration of the touch-screen interface using the
calibration wizard. If the calibration has varied click on this button and follow the instructions to re-calibrate the
screen. If you are having difficulties navigating to this option because the touch screen is out of calibration then
you may use a USB mouse plugged into the front USB port to reach the touch screen calibration wizard.
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7.1 User Accounts
In the users area you are able to select an existing user or create or delete a user profile. When a user is selected
thermal profiles will be loaded or saved to a directory specific to that user. Logging out will change back to default
user. To create a new user select the 'Add New User' icon and then enter the user name and choose a user profile
image.
7.2 File Manager
The file manager area enables you to transfer PCR run files between the default or individual user directories and a
USB memory drive. Plug your USB drive into the connector on the front of the unit and you may browse the
directories on the SC and transfer files to or from. You may also delete any thermal profiles from the SC. Press on
the 'Home' icons to refresh the directory list back to the root directories. Select files in either the SC or the USB
stick and press the transfer logos to copy the files across. Files may be moved between different user directories
by first copying them to a USB stick and then back into the destination user directory.
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7.3 Machine Settings
The following features are available under this page.
96 well thermal block settings:
'Hold at last temperature on run completion' will maintain the last profile temperature after the run has
completed. When this is not selected (default) the peltiers will deactivate at the end of the run and the
temperature of the samples will come to rest at approximately the internal heat sink temperature.
Typically this will be about 35deg C at the completion of a run. It is preferred to leave this option unselected as it will provide for a longer peltier service life.
'Audible alarm on run completion' toggles whether there is an audible alarm or just a screen message to
alert the operator that the run has completed.
'Use Predicted Temperature for Hold times' The SC software implements a thermal model which predicts
the actual liquid temperature inside the reaction vessels. Hold times can be based on this model (default)
or based purely on the block temperature. Using the predicted temperature effectively increases the hold
times (and total run time) slightly but gives a more accurate representation of the hold time of the actual
sample liquid. Most manufacturers thermal cyclers purely use the block temperatures for timing.
'Auto Resume Run After Power Outage' If this feature is available on your cycler (not available on all
models) then when this box is checked a run will automatically resume at the same place if power should
be momentarily lost during a run.
Top heater evaporation control settings:
'Use Top Heater' will cause the top heater to be used during a run if it is checked.
'Wait for top heater' will cause the system to wait for the top heater to achieve target temperature before
commencing a run. While this is happening a screen displaying the top heater temperature is displayed
and the user has the option of skipping this step.
'Leave heater enabled on run completion' will cause the top heater to be left on indefinitely at the end of
a run. This is useful if you are holding the samples at a high temperature on run completion.
Entry box for top heater temperature. Temperature is programmable between 30 – 110deg C (default 105
deg)
Button for manually enabling the top heater to preheat it if required.
'Disable top heater when set temperature < 30deg' feature is implemented to reduce the high thermal
load placed on the peltiers when holding the samples at low temperatures (i.e. 4degrees). This prevents
excess heat from the top heater heating the chilled samples. It is best to leave this option enabled.
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Note that the top heater is only activated when a run is started or the operator enables it in manual mode.
The 'Region Configuration' button enables the Operational region to be changed should the instrument
be moved to a different country. Note that available features vary between different regions. See Section
2.1 for more information.
7.4 Software Update
A facility has been provided to update the software which resides on the SuperCycler. The upgrade file you receive
from our support team or the website should be placed on the main root directory of your USB memory stick. You
may then select it to upgrade the unit’s software. After selecting the software file read the message box and press
OK and wait. Upgrade will take up to 1 – 2 minutes. Once upgrade is complete you can check that the new version
is installed by visiting the 'Information' page from the home screen and check the installed software version.
IMPORTANT NOTE: Do not interrupt the unit while a file upgrade is in progress. Switching off the power during
upgrade may render the unit inoperable.
After upgrading software the internal on screen user manual may need to also be updated. Click on one of the
help icons, it will display an upgrade message if a user manual update is also required. To update it you need to
download the correct user manual zip file from our website and unzip it on to the root (main) directory on a USB
memory stick and then insert it into the SuperCycler and follow the upgrade prompts when you try to access the
out of date help system.
7.5 Games & Extras
Under the extras tab you will find some other programs. The Oligo Calculator is useful for calculating the
theoretical melt temperature and other information pertaining to the oligo design. Simply enter the sequence and
it does the rest. Several popular Games are also provided for your enjoyment!
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8. User Tips
It is recommended before you use the device for the first time with biological samples that you experiment with
setting up runs and trialling these to familiarise yourself with the machine to ensure correct operation on your
valuable samples.
To achieve the maximum lifespan and best clarity from you SuperCycler touch-screen please only use the stylus
provided (or equivalent) or your fingers for selecting on the touch-screen. Using a ball point pen, pencil or
anything sharp will cause the screen to scratch.
To maximise the lifespan from the peltier devices which effect the heating and cooling there are a number of
actions the user may take:
Do not run the unit with no tubes in the plate and the lid closed. When the lid is closed and no tubes are present
the top heater rests close to the 96 well block and will place high thermal load on the peltier devices. Extended
operation in this situation will shorten the lifespan of the peltier devices.
Attempt to minimise the time holding the samples at below ambient temperatures longer than required. It is
recommended against using the device as a refrigerator to hold samples at 4 degrees C temperature for long
periods of time if you wish to maximise the lifespan of your instrument.
The heated lid applies substantial pressure to the tubes to ensure they are well seated into the thermal block and
prevent the caps or sealing film from popping off with the increased vapour pressure inside the tube. When using
only a single or small number of individual tubes then it is advised you place an empty tube in each of the 4
corners of the block to help absorb the pressure. This will help prevent budget, or thin wall plastic ware from
deforming under the high temperature and pressures.
The Top Heater evaporation control default is 105deg C. This is suitable for most applications. If you find that you
are getting excess condensation forming at the top of your tubes you can try increasing this temperature to
110deg C or higher.
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9. Specifications
Thermal Cycling Technology
Temperature Range
Temperature Accuracy
High performance active heating and cooling using quality
elements x 8 & precision sensors x 4
4°C–99°C
±0.5°C of set temperature, 1 minute after target
Temperature Uniformity
±0.5°C, 30 seconds after target
Temperature Resolution
0.1°C increments
Heating/Cooling Rate
Well Configuration
Linear thermal gradient
(Available selected regions only)
Condensation control
Heated Lid Temperature Range
Dimensions
3.25°C/sec maximum (block)
96-Well block supporting: 0.2 mL tubes or strip tubes with flat or
domed caps; 96-well high- or low skirt plates with strip caps, adhesive
cover, or oil overlay
Programmable 0 – 20°C across block width (12 wells)
Automatic utilising applied pressure heated lid
Controllable 60°C–115°C
W 180 mm
D 270 mm (10.6”); 340 mm (13.4”) including cables
H 190 mm lid closed (320 mm (12.6”) lid open)
Weight
5.5kg (12.1 lbs)
Colour
Titanium on frosted black
Electrical
100–240 VAC @ 4 Amp (50/60 Hz) Automatic voltage sense
Standard IEC Inlet plug
External Interface
USB interface to Windows XP / Vista based PC
USB host port for memory stick, mouse, keyboard etc.
Internal Interface
Embedded graphical controller with 7” widescreen touch sensitive
colour backlit display
Software
Supplied with unlimited user license. Free upgrades available via web
download.
Functionality
Touchdown, long range, linear thermal gradient, program pauses,
temperature graphing, temperature logging (PC only), Profile load &
saving. Manual mode
Included Accessories
Warranty
Kyratec SuperCycler User Manual v3.0
Power Cable, User manual, Stylus
Check with your local distributor
20
10. Terms of Use
END USER LICENCE AGREEMENT
Kyratec SuperCycler SC200
IMPORTANT: THIS END USER LICENCE AGREEMENT (or "EULA") IS A LEGAL AGREEMENT BETWEEN THE PERSON,
COMPANY, OR ORGANIZATION THAT HAS PURCHASED THIS EQUIPMENT ("YOU" OR "CUSTOMER") AND KYRATEC.
BY USING THIS DEVICE, CUSTOMER AGREES TO THE TERMS OF THIS AGREEMENT. IF YOU DO NOT AGREE TO BE
BOUND BY THESE TERMS, OR DO NOT HAVE AUTHORITY TO BIND CUSTOMER TO THESE TERMS, THEN DO NOT
CONTINUE, AND RETURN THE DEVICE TO YOUR PLACE OF PURCHASE FOR A REFUND IN ACCORDANCE WITH ITS
REFUND POLICIES.
1. Definitions:
(a) “Supplier” means Kyratec or one of its distributors.
(b) “Device” means Kyratec SuperCycler SC200 Thermal Cycler.
(c) “Region” means the country, group of countries or area in which device is located.
(d) “Features” means software or hardware operational functionality.
2. License Grants
Customer is only granted rights to use the device features which are offered to them in the region in which device
was purchased and resides. Customer warrants that the selected region is the true region of its purchase and
physical operational location. Incorrect selection of region is in breach of supplier terms of license and may or may
not breach any 3rd party license terms. Customer accepts sole responsibility for incorrect region selection and
consequences of any breach of 3rd party intellectual property associated with said features.
3. Limitation of Liability
Use of device and any results or information generated by device are at the sole risk of customer. Supplier makes
no guarantees that expected functions are performed correctly. Supplier strongly recommends that customer
perform full manner of internal controls within their reaction volumes to satisfy themselves that expected
functions were performed correctly. Supplier’s sole responsibility is for repair or replacement of defective device
within the warranty period. Maximum liability of supplier in any circumstance is limited to the amount paid by
customer to the supplier for the device.
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