User Manual
MicroFluidics Control System
User Manual
MFCS software©2006 Fluigent
MFCS User Manual version 1.2 www.fluigent.com
-1-
MFCS User Manual version 1.2 www.fluigent.com
-2-
Contents
1. Introduction .....................................................................................4
1.1.
Required Computer Hard and Software ............................................................. 5
1.2.
Package Contents ................................................................................................ 5
2. Initial Setup Operations..................................................................6
2.1.
Quick start ............................................................................................................ 6
2.2.
Front Panel Description....................................................................................... 8
3. Software Description ......................................................................9
3.1.
Pull down menu ................................................................................................. 10
3.2.
Graph display..................................................................................................... 10
3.3.
Status indicator .................................................................................................. 10
3.4.
Purge Buttons .....................................................................................................11
3.5.
Run Buttons ........................................................................................................11
3.6.
Automation of Protocols ................................................................................... 14
Appendix A: Technical Specifications MFCS ....................................15
Appendix B: Unit Conversion Table...................................................17
Appendix C: Hydrodynamic Resistance of Rectangular Channels
and Networks ......................................................................................18
Appendix D: Maintenance ..................................................................20
Appendix E: Trace and Log files format ............................................21
MFCS User Manual version 1.2 www.fluigent.com
-3-
1. Introduction
The MFCS is a compact, high precision pneumatic pressure controller designed for reliable
flow control in Lab-on-a-Chip applications, i.e. in microchannels, nanochannels and
capillaries. It allows the generation of stable, pulsation-free flows, and dynamic coupling as
well as automation of complex flow patterns in microchannel networks. It involves state-of
the-art electronic and pneumatic technology, and user-friendly software based on
FLUIGENT’s proprietary FASTAB technology. Different versions and configurations are
available – 4 or 8 channels (Figure 1) versions, equipped with 4 different pressure ranges:
25, 69, 345 and 1035 mbar. Standard 10-32 UNF thread output connectors allows
connection with numerous adapter-systems.
Figure 1: Top Left: 8-channels MFCS; Bottom left: 8 channels MFCS with pneumatic
connections. Right: Typical experimental microfluidic work station consisting in the MFCS, a
chip holder, and an epi-fluorescence microscope (computer not shown).
The MFCS is a high precision device, solving many of the problems encountered in
microfluidics, such as long equilibration times, hysteresis, unwanted hydrostatic flows,
difficulties in controlling simultaneously several interconnected channels, flow pulsing. The
MFCS provides extremely precise pressure regulation with small increments (16 bit) and an
short time constant, stabilized by a pressure feed back loop. Since the MFCS is pressurebased, and pressure is an intensive variable, it retains its accuracy independently of the
scale of the controlled microchannels network, making it particularly powerful for small scale
microfluidics and nanofluidics. The range of potential applications in biology includes e.g.
single molecule or single cells manipulations, kinetic studies, flow addressing and generation
of micro-gradients, automated and reproducible manipulation of buffers, samples and
separation matrices in analytical applications and bio-assays. Potential applications in
Chemistry and Physics involve e.g.: fundamental microfluidics, nanofluidics, studies of
instabilities and mixing in micro-channels, combinatorial chemistry, chemistry of explosives
(back pressure), local small-scale synthesis of toxic compounds, microfluidic-scale chemical
synthesis, emulsions, crystallization.
MFCS User Manual version 1.2 www.fluigent.com
-4-
1.1. Required Computer Hard and Software
−
−
−
−
USB 1.1 (no additional driver required),
Screen resolution 1024x768,
Minimum P II 500 MHz (if no other applications are running in parallel),
Operating system: default configuration of Windows 98SE, Windows2000, Windows
XP.
1.2. Package Contents
The MFCS package comprises following items:
− MFCS unit,
− Power supply + power line cable,
− USB cable,
− Pneumatic connection kit,
− User manual,
− MFCS Control software on CD
− Fluiwell (if required)
If any parts are missing or damaged, please contact your local
dealer or Fluigent immediately.
MFCS User Manual version 1.2 www.fluigent.com
-5-
2. Initial Setup Operations
2.1. Quick start
Figure 2: Front and back sides of the MFCS 8C. The front side consists of an input pressure
display monitoring the value and stability of the external pressure supply. The red button
resets the system, whereas the green button (re)starts it. The output connectors are of 1032UNF type. The input pressure connector, the USB and power (24Vdc)-connector as well as
the on/off switch are found on the back side.
1. Install the MFCS in a clean and dry environment and avoid exposure to corrosive
liquids and vapors or organic solvents which may attack the case. Note that the
MFCS is a purely pneumatic device – there should not be any contact with liquids at
any step of operation.
2. Plug the power cable into the power line, and the outlet of the power supply to the
24V inlet of the MFCS (rear panel). Connect the USB-cable to the dedicated
computer and to the USB inlet of the MFCS (rear panel). Only one MFCS can be
connected to one computer. Switch the MFCS on. The red front-panel LED schould
light up. Press the green start-button on the front panel to activate the system. When
the green LED lights up the system is ready for operation (figure 2)
3. Install MFCS Control software: in order too keep the installation and compatibility as
simple as possible the software consists of one single executable file, “mfcs VX.XXX.exe”. Please copy it from the CD delivered with the MFCS or from
www.fluigent.com. Please also copy the user manual in pdf format in the same
directory as the MFCS Control program, allowing direct access to the manual from
the MFCS. Start MFCS by clicking on the MFCS Control icon.
4.
Calibration:
please verify that no pressure input is connected to the MFCS, and
that output connectors of the MFCS are all open to a free, quiet atmosphere. After a
heating up time of approx. 10 minutes select in the set up pull down menu the
compensate offset item and follow the indications (figure 3). Calibration is required
only once for first operation. However it is recommended to recalibrate the MFCS
once a month or after moving or servicing the device.
MFCS User Manual version 1.2 www.fluigent.com
-6-
Figure 3: The calibration window is opened if compensate offset has been selected. The first
column displays the current pressure value, the second column shows the currently
programmed value and the last column averages the MFCS value over 1 second. Pushing the
Save to MFCS button writes the new values into the MFCS processors.
5. Connect a pneumatic pressure source with only dry, dust and oil free, non-corrosive
gases to the inlet at the rear of the MFCS. Use any elastic tubes of about 4-6 mm ID.
6. Please use a regulator adjusted to the pressure prescribed for your own version of
the apparatus (Table 1).
prescribed pressure
MFCS 25 mBar
MFCS 70 mBar
MFCS 350 mBar
MFCS 1000 mBar
500 mBar
500 mBar
800 mBar
1300 mBar
maximum
Pressure
600 mBar
600 mBar
900 mBar
1400 mBar
Table 1: prescribed and maximum pressure inlet for the MFCS devices
N.B: when you open the four channels simultaneously, the initial pressure is
decreasing from 10 to 15% depending on the air flow of the pressure source.
7. Connect your application to the MFCS and start your experiment. The outlets are
provided with 10-32 UNF type1 threaded connectors.
8. Have success with your MFCS experiment.
IMPORTANT: No liquids schould enter the MFCS. Liquid backflow into the MFCS will
void guarantee. To avoid liquid backflow, the Fluiwell, also being a reservoir, or a similar
protection system is recommended. If sample and reagent reservoirs are integrated on the
chip the fluiwell can also be used just as intermediate liquid trap.
1
The 10-32 thread can also receive connectors with European M5 threads, even though we
don't guarantee for tightness and reliability of those connections.
MFCS User Manual version 1.2 www.fluigent.com
-7-
2.2. Front Panel Description
The front panel carries an input monitor display, red and green buttons, red and green LEDs
and 4(8) pressure outlets (figure 2).
The RED BUTTON allows to stop and reset the system immediately (emergency stop) and
put it into the “standby” state. In this state the security valve disconnects the input pressure
source from the pneumatics of the MFCS, and all outlets channels are closed.The system
starts up in the “standby” state. The GREEN BUTTON allows to (re)start the system and to
establish the USB communication with the connected computer.
The LEDs have following meaning:
1.
2.
3.
4.
RED LED on: standby status,
GREEN LED on: system operational,
GREEN LED blinking: purge activated,
GREEN and RED LED blinking alternatively: input exceeds limit value.
MFCS User Manual version 1.2 www.fluigent.com
-8-
3. Software Description
The MFCS Control software allows numerous operations as well as a basic programming
capability (for more complex operation, we recommend the use of the optional MFCSmat
stand-alone scripting software). The six labeled fields in the graphical window comprise: the
graph display (top left), the slider cursors (top right), the status (bottom left), the Run and
Purge button (bottom center), the numeric block (bottom right) and configuration selector.
Figure 4: The MFCS Control software consists in 6 main fields (fields limited by red dashed
line) and two additional control buttons. The pull-down menus are used for file and
configuration operations, calibration, and provide information about the system. The display
field is dedicated to the trace of the output pressure.
MFCS User Manual version 1.2 www.fluigent.com
-9-
3.1. Pull down menu
File menu operations
“SAVE”, “SAVE AS”, and “OPEN”
The current configuration parameters that includes all the configurations definition can be
stored by selecting the Save or Save as item and read with the Open item. The datas stored
are in the ASCII format and can be edited with notepad or automatically created. The format
is self-explaining.
“SAVE LOG”
The Save log option allows to trace back all executed operations and to analyze them in
retrospect.
“SAVE TRACE”
The Save trace option allows to storage the measured data in a text file. It is further possible
to select different acquisition rates (0.1s, 1s and 10s).
Calibration menu operations
See “Quick Start” item 4 in page 6.
Help menu
The Help menu contains an “ABOUT” item informing about the contact address of the MFCS
developers, and about software and firmware version numbers.
3.2. Graph display
The graph window shows the measured pneumatic pressures as a multicolor graph. The
values can be recorded to a file using the “SAVE TRACE” option (Pull down menus
described above).
3.3. Status indicator
The MFCS status field (bottom left) shows the status of:
− The USB connection,
− The system state (standby or active, as selected by operation of the red and green
buttons on the MFCS fornt panel),
− Suspended operation due to input pressure exceeding the maximum input pressure (as
indicated in the technical specifications),
− Purge activation (as set by “purge button”, see below).
MFCS User Manual version 1.2 www.fluigent.com
- 10 -
3.4. Purge Buttons
Pushing the purge button bypasses the regulation and forwards the input pressure
directly to the outlet 1. This operation is very useful for fast purging the system, filling it
with viscous buffers (e.g. electrophoresis matrices), removing dust, bubbles, etc.
IMPORTANT: Never use this function if you are not sure that your microfluidic system
will withstand the input pressure of the MFCS.
3.5. Run Buttons
Pushing the Direct control button (mouse click) alternates between the “DIRECT CONTROL”
and the “SETTING” mode.
In the “DIRECT CONTROL” mode (button white and activated), modification of the control
slider buttons (see below) by the user are transmitted straight to the MFCS output.
In the ““SETTING” mode (button gray), the new settings of the control slider buttons are
stored, but regulation still operates with the values displayed when the system was switched
from the “direct control” to the “setting” mode. The new values are taken into account only
when the Direct control button is pressed again. This permits to preselect pressure values and
apply them simultaneously to the ouput channels. Upon start, the system is in “setting”
mode.
Control slider buttons
4 (8) slider buttons are employed to select independently the different output pressure levels.
The sliding bar range corresponds to the range of the pressure sensors. The selected slider
buttons can be controlled in different ways:
1. Mouse or other pointing device,
2. Cursor up and down keys (finest possible step size: 65536 steps over the whole
range),
3. Page up and down keys (coarse step size: 100 steps over the whole range),
4. End and home keys (jumps to 0 or to maximum pressure),
5. Input numerical value in the Requested Pressure box (auto mode) or Valve Output box
(direct mode) in the numeric block (see below for “auto” and “manual” modes).
Pressure is automatically regulated to the requested pressure if the system is in “direct
control” mode (Direct control button white) and the channel in “auto” mode (see below).
Control coupling: parallel coupling
It is often useful to couple the pressures in different channels, e.g. to increase the global
pressure in a network while keeping the flows in the different channels fixed (“parallel
coupling”) or to adjust the relative flow rate in two channels, without changing the overall
combined flow (“anti-parallel coupling”). This can be achieved by pushing the down-arrow on
the right side of the coupling window in the Numeric Block frame. This calls a menu: A+, A-,
B+, B-.
Selecting A+ on channel 1 and A+ on channel 2 locks the two slider buttons in a parallel way
– they move together in such a way that the difference remains constant. When the shift key
is pressed during slider motion, the coupling is suspended and the concerned slider button
MFCS User Manual version 1.2 www.fluigent.com
- 11 -
can be moved independently of the other slider buttons again. It is further possible to couple
more than 2 channels. A and B allows to couple independently two groups of channels.
Control coupling: antiparallel coupling
The anti-parallel coupling is achieved by setting one channel to A+ (B+) and a second to A(B-). Then increasing/decreasing of one channel-value results into the decreasing/increasing
of the other while keeping the algebric mean value constant. This is useful, for example for
modifying the relative flow in two channels (of equal flow resistance) merging into a third
channel in which the flow should not be affected (e.g. changing the mixing ratio of two
reagents without changing the flow downstream). Only two channels can be coupled in antiparallel sense.
Parallel and anti-parallel couplings can be combined freely.
Figure 5: Anti-parallel coupling allows changes in the pressure-difference of two channels
without changing the overall pressure. Parallel coupling is needed if e.g. the injection rate of
two different liquids needs to be changed simultaneously. Both function can be combined.
Numeric bloc
This block provides useful information on the settings and measured values for each
channel.
Maximum pressure: The maximum pressure that each pressure sensor can measure is
displayed in the first line.
Requested pressure: This field displays the requested pressure corresponding to the slider
button position. It is also possible to enter numerical values directly, by clicking in this box
and typing the desired value on keyboard.
Measured pressure: The value displays the actual measured pressures. This allows a real
time monitoring of the regulation, and diagnosis of malfunction (see below). The time
evolution of this value is shown for all channels in the graphic display – each channel in a
different color
Manual/Auto: Two different pressure generation modes can be chosen: The manual
(manual) and the automatic mode (auto). The normal operation mode is auto. In this mode the
FASTAB technology provides constant pressure values. The requested pressure is set, and
MFCS User Manual version 1.2 www.fluigent.com
- 12 -
the MFCS dynamically maintains the pressure at the set requested value, irrespective of the
changes occurring in the microfluidic network.
In the manual mode the slider button’s position (or numerical setting in the “valve output” box)
is directly converted into the electrical control current to the regulator valve (important: note
that in this case, it does not represent a pressure value). The use of the manual mode is
discouraged, since it does not benefit from the powerful features of the auto mode, for
dynamical compensation of the intrinsic nonlinear characteristics, hysteresis and limited
reproducibility of the solenoid-valves. Only special applications may require to switch to
“manual” mode, e. g. if the connected system is highly instable, if other control variables than
pressure have to be stabilized, or for unraveling anomalous operation of complex networks.
Feed Back Coefficient (auto mode only)
The MFCScontrol software provides optimal pressure and flow regulation in a transparent
way to the user in a wide range of situations covering the majority of microfluidic
applications, in particular those involving extremely low pressures and or volume flow rates
(µl to pl/min). Optimal operation of the MFCS for extremely high or low pressure (flow rates)
may require an adjustment of the reactivity of the feed-back loop. This can be achieved,
independently for each channel, by changing the setting in the “feed back coefficient” box.
The factory setting (50) should be appropriate for most applications. Too small coefficients
(e. g. 5 for the 25mbar version) result in a slower reaction, high values (>200) can destabilize
the system in particular when large pressure changes are requested abruptly. The maximal
value is 255. The optimal response is obtained just below the critical damping situation
between a monotonous return to equilibrium and oscillatory overshooting. Please note that
for high pressure versions of the
MFCS, at the factory-setting initial value, operation is oscillation-free for small pressure
changes but shows overshooting and transient oscillations for drastic pressure changes. If
this overshooting is a nuisance to your application, it can be suppressed by decreasing the
feedback coefficient, at the expense of the speed of regulation for small pressure changes.
A working compromise has to be found depending on the specific application. The optimal
value depends on several features of the connected system (volume and elasticity of tubing,
hydrodynamic resistance and geometry of the microfluidic channels), and on the time
constant of the phenomena under investigation.
Configuration folders (“CONFIG”)
Microfluidic experiments often involve switching between different sets of predefined flow
configurations.
MFCScontrol allows you to define and store up to 8 different configurations. Switching
between configurations is performed by clicking on the corresponding folder button at the
bottom of the numeric block. In the direct control mode (”Direct control” button white) switching
between different configurations is immediately effective on the output pressures.
In the “setting” mode (“Direct control” button inactive) the pressures corresponding to the
selected configuration are effective only after the “Run” button has been pressed.
Active config: This display shows the current number of the currently active configuration
folder.
MFCS User Manual version 1.2 www.fluigent.com
- 13 -
Stand alone operation
For long operation times under fixed pressure settings, the computer can be disconnected
from the MFCS unit after switching to the setting mode. The system continues maintaining
the previously set pressure values. Any modification of these values needs to connect the
computer again. To avoid any risk of uncontrolled pressure variations in the
microfluidic system, please (re)connect and disconnect only in the setting mode
(Direct control switched off).
3.6. Automation of Protocols
For long period or complex operation the MFCSmat software is recommended. This permits
to program in a convenient way protocols and automate efficiently complex experiments.
Further, coupling with other software is possible.
MFCS User Manual version 1.2 www.fluigent.com
- 14 -
Appendix A: Technical Specifications MFCS
MFCS 25 mBar
Characteristics
Input pressure range
Output pressure precision
Min.
-
Typ.
500
<2.5 %
Max.
600
-
Unit
mbar
full scale
Min. output pressure step
Max. output pressure
Operation temperature range
Input voltage range
Weight
Power consumption
-10
23
1.9
-
23
25
25
24
-
50
25
2.25
<16
µbar
mbar
°C
V
kg
W
Comment
Better if calibrated
(see Quick start)
Depends on model
MFCS 70 mBar
Characteristics
Input pressure range
Output pressure precision
Min.
-
Typ.
500
<2.5 %
Max.
600
-
Unit
mbar
full scale
Min. output pressure step
Max. output pressure
Operation temperature range
Input voltage range
Weight
Power consumption
-10
23
1.9
-
63
69
25
24
-
50
25
2.25
<16
µbar
mbar
°C
V
kg
W
MFCS User Manual version 1.2 www.fluigent.com
Comment
Better if calibrated
(see Quick start)
Depends on model
- 15 -
MFCS 350 mBar
Characteristics
Input pressure range
Output pressure precision
Min.
-
Typ.
800
<2.5 %
Max.
900
-
Unit
mbar
full scale
Min. output pressure step
Max. output pressure
Operation temperature range
Input voltage range
Weight
Power consumption
-10
23
1.9
-
0.32
343
25
24
-
50
25
2.25
<16
mbar
mbar
°C
V
kg
W
Comment
Better if calibrated
(see Quick start)
Depends on model
MFCS 1000 mBar
Characteristics
Input pressure range
Output pressure precision
Min.
-
Typ.
1.3
<2.5 %
Max.
1.4
-
Unit
bar
full scale
Min. output pressure step
Max. output pressure
Operation temperature range
Input voltage range
Weight
Power consumption
-10
23
1.9
-
0.95
1033
25
24
-
50
25
2.25
<16
mbar
mbar
°C
V
kg
W
MFCS User Manual version 1.2 www.fluigent.com
Comment
Better if calibrated
(see Quick start)
Depends on model
- 16 -
Appendix B: Unit Conversion Table
value (↓
↓) = factor × kPa
unit (→
→)
1 kPa
1
1 bar
100
1 psi
6.895
1 inch H2O
0.249
bar
psi
inch H2O
0.01
1
-3
68.95×10
-3
2.49×10
0.145
14.5
1
3.612×10-2
4.016
401.6
27.68
1
Example: 10 kPa = 10 kPa × 0.01 bar/kPa = 0.1 bar
MFCS User Manual version 1.2 www.fluigent.com
- 17 -
Appendix C: Hydrodynamic Resistance of Rectangular Channels
and Networks
Pressure difference ∆p, the volume flow rate Q, or the averaged velocity u and the friction
coefficient C are related through
-4
with channel dimensions a and b with a/b>>1, channel length l and viscosity µ (= 9.5 × 10
Pa s for H2O at 25°C).
The relation between the friction C and the aspect ratio ξ= a/b can be calculated [Stone,
Stroock, Ajdari, Ann. Rev. Fluid. Mech., 2004]:
with
Figure 6: This graph shows the friction coefficient as a function of the aspect ratio of
the channel and can be used to estimate the flowrate due to a given pressure
difference. Typical values are: ξ=1 -> C=0.03512; ξ=2 −> C=0.05708; ξ=10 ->
C=0.07688.
Example: A channel of 10x100µm with a length of 10mm contains 0.01 µl. Applying a
pressure difference of 10 Pa (=100 µbar) gives a volume flow rate of about Q=0.0005 µl/min
MFCS User Manual version 1.2 www.fluigent.com
- 18 -
and an averaged flow velocity of u = 5 µm/min. A pressure of 100'000 Pa (=1 bar) is 10'000
times higher thus the flow rate and velocity are also 10'000 times increased.
Since Ohm's law also applies to flow of other particles than electrons (e. g. water) we can
transfer the formulas.
For one single channel we have for the volume flow Q as a function of the externally applied
pressure difference p and the resistance R: Q=p /R, Q=abu.
The pressure pc at an arbitrary position in a single channel with partial resistances R1 and R2
of the two halves of the channel is:
The total resistance is R=R1 R2, the flow yields Q= p1−pc /R1, R2 is equivalent.
If the two parts are of equal resistance (equal length and diameter) we find the well known
average value: pc= p1 p2 /2.
For three channels (p1, p2, p3 and R1, R2, R3) joining in one point with pressure pc we find
using the continuity equation (the sum over all Qs going into one knot vanishes)
The corresponding flow in channel 1 as an example can be obtained with Ohm's law:
Q1= p1−pc /R1, the other values are equivalent.
To obtain the corresponding formulas for n channels joining in one knot, the latter formula
can be generalized:
More complex geometries can be calculated using well-known methods in analogy to
electrical resistor networks (pressure is equivalent to the electric potential, flow is equivalent
to the current, and flow resistance of a channel plays the same role as the Ohm's
resistance).
MFCS User Manual version 1.2 www.fluigent.com
- 19 -
Appendix D: Maintenance
The MFCS is designed as a very robust device for usage in laboratory and industrial
environment and easy to maintain. For optimal accuracy, calibration should be performed
regularly (typically every month) or after the MFCS has been displaced, in order to maintain
optimal accuracy of the results and compensate for offset drift of the pressure sensors. The
housing can be cleaned with a moisty soft tissue, iso-propanol or window-cleaning products.
Avoid aggressive organic solvents as acetone or chloroform or abrasive cleaning products.
Please use the following form if you have problems or suggestions with your MFCS device or
software and send it to us by fax or e-mail.
MFCS User Manual version 1.2 www.fluigent.com
- 20 -
Appendix E: Trace and Log files format
MFCScontrol allows a complete real time recording of all settings and measured pressure
values during operation.
11.07.2005 17:39:03 MFCS S/N:0003 V:000.011
15885
18.424
0.426
5.146
20.560
15886
18.424
0.426
5.100
20.477
15887
18.386
0.395
5.100
20.454
15888
18.371
0.426
5.123
20.583
15889
18.485
0.395
5.100
20.583
15890
18.462
0.426
5.100
20.500
15891
18.402
0.426
5.146
20.500
15892
18.333
0.395
5.123
...
Figure 7: an example of a Trace file.
The trace file format consists of a header line and a data block. The header line is composed
of the starting date and time, serial number of the connected unit and version number of the
MFCScontrol software. The data block is composed of lines carrying a time stamp (absolute
time and the internal step number) and the 4 (8) pressure values. '#N/A' means information
lost (e. g. transient transmission failure). When using this option, avoid the creation of
oversized data files. Adjust the storage frequency according to the needs (see pull down
menus).
11.07.2005
11.07.2005
11.07.2005
11.07.2005
11.07.2005
11.07.2005
11.07.2005
11.07.2005
11.07.2005
11.07.2005
11.07.2005
11.07.2005
11.07.2005
11.07.2005
17:27:38
17:27:43
17:27:43
17:27:43
17:27:43
17:27:43
17:27:43
17:27:43
17:27:45
17:27:45
17:27:46
17:27:46
17:27:46
17:39:27
--BEGIN OF LOG-AUTO.
Chan.
AUTO.
Chan.
AUTO.
Chan.
AUTO.
Chan.
AUTO.
Chan.
AUTO.
Chan.
AUTO.
Chan.
AUTO.
Chan.
AUTO.
Chan.
AUTO.
Chan.
AUTO.
Chan.
AUTO.
Chan.
--END OF LOG--
1
1
1
1
1
1
1
1
1
1
1
1
:
:
:
:
:
:
:
:
:
:
:
:
2196
2221
2246
2283
2332
2381
2406
2715
2727
2739
2739
2739
Figure 8 : an example of a Log file.
MFCS User Manual version 1.2 www.fluigent.com
- 21 -
ALPHA
AUTO
Chan. X : YYYYY
Chan. X :YYYYY
--BEGIN OF LOG---END OF LOG-MANUAL Chan. X : YYYYY
PURGE OFF
PURGE ON
USB OFF
USB ON
ZERO Chan. X : YYYYY
Order: set α value to YYYYY in Channel X
Order: set value YYYYY in Channel X in
feed back mode
Order: start logging
Order: stop logging
Order: set value YYYYY in Channel X in
manual mode
Order: open purge valve
Order: close purge valve
Order: close USB channel
Order: open USB channel
Order: set zero value YYYYY on channel X
Table 2: commands sent from the computer to the MFCS.
mfcs exceeding input pressure
mfcs operating
mfcs purge off
MFCS S/N:---- V:---mfcs purge on
mfcs reset detected
Message: input pressure exceeds threshold
value
Message: system is operating normally
Message: purge is switched off
Message comprising serial and version
number
Message: purge is switched on
Message: system has realized activation of
reset signal (button, processor reset line)
Table 3: commands sent from the MFCS to the computer.
.
MFCS User Manual version 1.2 www.fluigent.com
- 22 -
Fluigent
tel. : +33(0)15373-1551
fax. : -1552
e-mail : [email protected]
MFCS
date :
SUGGESTIONS /DYSFUNCTIONS
Name : ____________________________________ Urgent :
Society : __________________________________
yes
Suggestion
Dysfunctions
Device/Version : ____________________________________________________
Software/Version : ____________________________________________________________
______
Menu or concerned function :
_______________________________________________________
__________________________________________________________
Error code, if available :
DESCRIPTION
(please cite error messages, operation before error, operation to quit error
message, add screen shots if possible)
Don't write in this field, please.
MFCS User Manual version 1.2 www.fluigent.com
- 23 -
Fluigent SA
4 Avenue de l’Observatoire
75006 Paris
France
Phone : 0033-(0)153731551
Fax: 0033-(0)153731552
web-page: http://www.fluigent.com
e-mail: [email protected]
MFCS User Manual version 1.2 www.fluigent.com
- 24 -
Was this manual useful for you? yes no
Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Download PDF

advertisement