analySIS
analySIS
Manual
CC-12
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CC-12
CC-12 II
The CC-12 - Camera
1
Handbook Layout
2
Camera and light microscope
2
WHITE BALANCE .....................................................................3
Monitor Settings for White Balance ................................3
Executing White Balance ...............................................4
ECC121104
Image Acquisition
5
ACQUISITION METHODS ........................................................5
Live-Acquisition ........................................................5
Snapshot ..................................................................5
intelligent Exposure ..................................................6
ACQUISITION ...........................................................................6
CAMERA CONTROL ................................................................6
Exposure Time .........................................................7
White balance ...........................................................7
Color Settings ...........................................................7
Sharpen filter ............................................................9
Automatic Gain Display and Scaling ......................10
Black balance .........................................................11
Sharpness Monitor .................................................11
SNAPSHOT .............................................................................13
INTELLIGENT EXPOSURE ....................................................13
CC-12
NetCam
16
INSTALLING THE WEB SERVER .......................................... 17
NETCAM IN THE INTRANET ................................................. 17
NETCAM IN THE INTERNET ................................................. 18
USING NETCAM ..................................................................... 19
Camera Set Up
21
SOFTWARE CAMERA CONTROL ......................................... 21
The Input tab ................................................................ 22
The Display tab ............................................................ 25
The Format tab ............................................................ 29
Appendix I: Microscope Settings
31
Appendix II: Camera Installation
35
INSTALLATION PROCEDURE - WINDOWS 2000 ................ 37
INSTALLATION PROCEDURE - WINDOWS XP ................... 49
WHEN ENCOUNTERING PROBLEMS WITH THE CAMERA...
57
CE DECLARATION OF CONFORMITY
59
The CC-12 - Camera
CC-12
The CC-12 - Camera
Definition
CC-12
Dynamic range 3 x 12 bit
1 x 12 bit using binning
Exposure times 100 µs to 160 s
Frame rates 22 / s @ Binning 2x
12 / s @ full resolution
Resolution 1376 x 1032 Pixel
Binning 2x, 4x
Cooling Peltier-cooling: 10°C @ 25° ambient
temperature
Camera Mount Standard C-Mount
PC-interface Firewire (IEEE 1394)
1
CC-12 is a quick, high resolution CCD color camera used for biomedical
and material sciences applications. It was especially developed for acquisitions using light microscopy, has a special compact design, and can be
connected to all types of light-microscopes via the C-mount. Its high dynamic scope enables it to acquire images which have very light and very dark
areas
The CC-12 offers the following technical specifications:
CC-12 • Handbook Layout
System Requirements
To properly use CC-12, the following system requirements for your computer and your image analysis system should be taken into consideration:
Hard- / Software
Required
CPU P4 with at least 1,8 GHz
Memory 256 MB
Hard Disk Storage 20 GB
Interface Firewire (IEEE 1394)
Operating system Windows 2000 Professional,
Windows XP Pro
analySIS analySIS 3.2 Build 625 or higher
analySIS FIVE
Modern cameras with the respective software offer numerous possibilities
for correcting and modifying the acquired image. They enable the image to
be optimized quickly. However, the entire system, the sample, the microscope with all its possibilities, and the camera all must be optimally set in order to attain the best possible results.
Handbook Layout
Camera installation
and setup
Camera and light microscope
2
Image Acquisition
This handbook will help you use your system to optimally acquire images.
Installation of camera, driver and corresponding software will be describes
in the appendix.
All basic camera settings are handled by the software via one or more logical
input channels. Options for the input channels are defined in the chapter
"Camera Set Up". This chapter is at the end of the book, since hardly any
alterations are made to the input channels in day-to-day use.
The chapter entitled "Camera and Light Microscope" portrays the elements
of the microscope which have the greatest influence on the image quality.
The correct microscope settings are explained.
The chapter entitled "Image Acquisition" deals with the uses and possibilities of a digital camera, as well as the software required for image acquisition. This chapter will be the most used chapter for day-to-day use. The individual acquisition methods (live-acquisition, single image, intelligent exposure) are explained in this chapter in detail.
Camera and light microscope
Both the camera settings as well as the microscope settings are vital for the
quality of the image to be acquired. Only the most important rules for the microscope settings are mentioned here; a more thorough explanation to the
topic can be found in the appendix:
1) The illumination must be parallel.
2) Use ND-filters to reduce the illumination intensity; do not alter the lamp
voltage.
3) Establish Köhler illumination conditions.
Related Topics
Camera Set Up 21
Camera and light microscope 2
Image Acquisition 5
Appendix I: Microscope Settings 31
White Balance
4) Select the appropriate total magnification and the objective for the
problem in question.
White Balance
Mode of operation
Standardized White Balance
Manual White Balance
Monitor Settings for White Balance
Monitor Settings
Note
How to...
The monitor settings are decisive for the image you see on the monitor. Set
your monitor to the appropriate color temperature, should your device allow
this. To do this, compare the image's color impression on the monitor and in
the eyepiece.
The color temperature setting can be altered on most monitors via the monitor menu buttons located on the monitor. In other cases, the color temperature can be altered via the operating system.
This is how you change the color temperature using the operating system:
1) Right click on Desktop.
" The Desktop's context menu is opened.
2) Select the Properties entry from the context menu.
3
Microscope Settings
The human eye reacts very sensitively to color variations which are perceived as a color tingeing effect. Therefore, when wanting to acquire quality
images with a digital camera, it is vital that the individual color channel settings are set so that the entire image does not show color tingeing. The adjustment of the color channels and the white balance depend on the illumination conditions. When changing the lamp or altering the lamp voltage, the
white balance should be repeated afterwards accordingly.
When using white balance, the individual color channels are scaled in a way
that the white or neutral gray area of the image displayed on the monitor is
displayed correctly as white or gray. The white balance can be carried out
automatically on each image or manually for individual images.
An area which is, for the most part, uniformly gray or white is selected on the
image. Such an area must contain an equal intensity of the three color components : red, green and blue. White balance sets the color channels in such
a way that the requirements for the selected area are satisfied as much as
possible. To be able to carry out an effective white balance, you need the
respective white or gray area.
White balance is carried out on a part of the image (ROI). The program alters
the color settings in such a way that the image area in the ROI appears as
white or gray as possible. After you have once carried out the white balance,
you can reapply it any time to a running live-image acquisition.
A test image is acquired with the camera using optimal illumination for the
standardized white balance. The individual color channels are now set so
that the acquisition also appears white or gray on the monitor. These channel settings are saved and used automatically for all additional acquisitions.
A manual white balance is carried out on the current single image / snapshot. To do this, you need an area in the image which is for the most part
gray or white.
Use the same microscope settings for the white balance that you will use for
the acquisitions later. This especially holds true for the filter and the lamp
voltage settings. The lamp voltage of a 12V halogen lamp should be set to
9V. It should, however, not be set lower than 5V. Use the color neutral NDfilter, should you have to reduce light intensity.
CC-12 • White Balance
Note
" The Display Properties dialog box is opened.
3) Select the Settings tab.
4) Click the Advanced... button.
" Another Display Properties dialog box is opened. The appearance
and function of this dialog box depends on the graphics card installed on your system and your monitor.
" Search for a tab with the name Color or Color Management.
The appearance of the operating elements might differ from the one being
described here because the adaptation of the color temperature is not part
of the operating system, but rather is made accessible by the monitor manufacturer or the graphics card being used.
5) Select a color temperature whose color impression best corresponds
to that seen in the eyepiece.
" Should your system offer a slide control with the options warm and
cold, do not alter your color temperature with these.
Executing White Balance
Specialized microscopy
techniques
Transmitted Light
Reflected light
4
How to...
It is very difficult to execute a white balance in some microscopic methods,
i.e. DIC or polarization. In these cases, execute the white balance using the
bright field method and only then begin with the special methods.
When using the transmitted light method, look for a position through which
the light passes through the cover slip, embedding material, and microscope
slide, but not through the specimen. Should there not be such an area, remove the object to be observed. Thus, the lamp light used for illuminating
the specimen falls directly on the objective. This is white when using a white
lamp.
When using the reflected light method, you need the appropriate reference
surface. Standardized gray cards are best for this. Should one not be available, you can also use white laboratory porcelain or neutral ND-filter paper.
Should your samples contain very reflective areas, defocus your microscope
when setting the white balance.
This is how you set up the white balance:
1) Acquire an appropriate snapshot.
2) Use the Image > Camera Control... command.
" The Camera Control dialog box is opened.
3) Click the Set ROI for White Balance button.
4) Pull the ROI's red frame shown in the image document to a position
which is more or less a uniform white or gray.
" Change the size of the area by keeping the mouse depressed and
moving the mouse. The ROI used for the white balance cannot be
made to any large size possible.
" The ROI can be difficult to see if the image document has a reddish
shade. In this case, use the Special > Preferences > View command to change the color of the frame. You can select a more appropriate color from the Primary list located in the Mouse cursor
group.
5) Rightclick to confirm position and size of the ROI.
6) Click the White Balance button to conduct an automatic white balance.
Related Topics
Camera Control 6
Image Acquisition
" The settings for the individual color channels are optimized in the
set area. The result is directly visible in the image buffer's image.
7) Click the Color Settings button.
" The Color Settings dialog box is opened.
8) Click the Define...button.
" The Define Default Color Settings dialog box is opened.
9) Click the Current settings button to adopt the current values for the rest
of the work.
" When using this setting, the white balance is valid for all images
which are acquired with this input channel during the current session.
10) Click the OK button to return to the Color Settings dialog box.
11) Click the Standard button to save these values as general standards.
" The current values for the white balance are now saved and will be
imported when the program is restarted. A manual change of the
color settings changes the white balance.
The program alters the color settings in such a way that the image area in
the ROI appears as white or gray as possible. After you have carried out the
white balance once, you can reapply it any time to a running live-image acquisition.
Image Acquisition
The chapter about "image acquisitions" deals with the various acquisition
methods you can use with the image-analysis software.
Acquisition Methods
The software offers you various acquisition methods for various tasks.
Live-Acquisition
Snapshot
The Image > Snapshot command concludes the live acquisition and saves
the current image to the active image buffer. The same settings which are
used for the live acquisition are also used for the snapshot. You can make
a snapshot without acquiring a prior live acquisition.
Related Topics
Acquisition 6
Snapshot 13
5
When using live acquisition, the image is continually transferred from the
camera to the monitor. This mode is suited for setting various parameters,
including image segments, focussing, and camera settings. The live acquisition is concluded after a snapshot has been acquired. You can use the Image > Camera Control... command to do numerous camera settings during
a live acquisition.
CC-12 • Acquisition
intelligent Exposure
The Image > intelligent Exposure... command offers you an extensively automated image acquisition. The automated functions being used coordinate
the amplification of the image signals and the exposure time of the acquired
image so that a good image result is guaranteed. The exposure time of a live
acquisition, through the resetting of the camera's acquisition mode, are kept
so short that a very good adjustability during the live-image is guaranteed.
Acquisition
Use the Image > Acquire command to display the acquired image on the monitor live.
You may also
Available
What will happen...
Why use live-images?
live-image settings
6
Editing other images in
live mode
Live overlay
Available
You may also click the Standard (button bar) > Acquire button.
The command is not available if the active image buffer is write protected.
The live-image is displayed in the active viewport. The camera symbol located in the status bar blinks and the name of the camera appears in red. As
long as the live-image is shown in the active viewport, the image-analysis
software only offers commands which can be effectively used.
You need the live-image for focussing, or when arranging the camera or the
sample. The Image > Snapshot command stops the live-image display and
takes the image last shown in the active image buffer.
You can determine the settings for the image acquisition in the logical input.
Use the Image > Set Input...and Image > Configure Input... command to create an input and to determine its properties.
The live-image is shown in only one viewport. If you are using two monitors
or have numerous viewports in your image window, you can enable another
viewport in live mode and at the same time load another image and display
it in this viewport, for example. In addition, you can measure another image
with the interactive measurement functions, save them, or insert them into a
database while live mode is active.
The Live overlay function enables you to write and draw directly onto the image's overlay during the live acquisition. To do this, use the buttons in Overlay button bar.
The live overlay is only available if you have activated it with the Image >
Configure Input command located on the Display tab.
Camera Control
Use the Image > Camera Control... command to alter the settings for acquisition with the camera.
The Camera Control command and the appropriate dialog box enables you
to attain control over numerous setting possibilities required for acquiring
single images and live acquisitions. The commands in this dialog box are
closely interlocked with the settings available from the Image > Set Input
command.
Related Topics
intelligent Exposure 13
Live overlay 27
Camera Control
Available
You may also
The Camera Control command is only available if the Set Input dialog box
is closed.
You can also click the Standard (button bar) > Camera Control button.
The Camera Control dialog
box for a CC-12 camera
Exposure Time
Exposure Time
Auto
The exposure time required for high-quality acquisitions depends on the illumination, the properties of the sample, and the camera being used. First,
remove possible ND-filters to be able to manage with lower exposure times.
You can also additionally set the prism or the phototube's mirror to "only
photo". Should the result not be adequate enough, you can then increase
the lamp voltage. After you have increased the lamp voltage, it is recommended that you redo the white balance.
You can set the camera's exposure time in the Exposure time group.
Click the - and + buttons to alter the exposure time in pseudo-logarithmic
steps. You can also directly change the exposure time in the corresponding
text field or manipulate it using the arrow buttons next to the text field. The
minimum and maximum exposure times depend on the camera being used.
Click the Auto button to enable the automatic exposure time settings. The
Auto button is only available in live-mode.
White balance
Color Settings
Click the Color Settings button to manually alter the camera's color settings.
Related Topics
White balance 7
7
The white balance proceeds after the description shown above .
CC-12 • Camera Control
Red, Green, Blue
Gamma
What is gamma?
Note
8
Note
Saturation
Default
Define…
The current settings can be altered using the five slide controls: Red, Green,
Blue, Gamma, and Saturation.
The Red, Green and Blue slide controls can each be set from 0,00 to 20,0.
The set value is the factor with which the signal of the corresponding color
channel is multiplied.
Use the Gamma slide control to obtain a nonlinear contrast enhancement.
Please note that this gamma filter does not only affect monitor display but
also alters actual image information. If you are planning on quantitatively
evaluating your images at a later time, you are advised to use the value 1.0
for the gamma filter. The Oper menu provides you with commands for adjusting image contrast at any later time.
As is the case with the slide controls of the individual color channels, the numerical value for gamma effects how the illumination intensity of the pixels
of the camera CCD chip is converted into the color value for the corresponding pixel in the image buffer. If gamma equals 1, the conversion from illuminance into gray/color value is linear. A nonlinear contrast enhancement
results should the gamma not be equal to 1: if gamma is smaller than 1, the
darker gray values will be spread more significantly than the brighter ones.
The result will be enhanced contrast in the lighter areas of the image. The
image as a whole will become darker. If gamma is greater than 1, the darker
gray values will be spread more significantly than the brighter ones. The result will be enhanced contrast in the dark image areas. The image as a
whole will become lighter.
Any gamma-value not equal to 1 has a special effect on images acquired using binning. The gamma-value set here will be transformed into a gray LUT
for black&white images acquired with binning. With binning activated, gamma can not be changed; the Color Settings is not active. The result is a falsecolor image. Use the Oper > Set Gray-LUT to remove the LUT from the image. The result is a grayscale image with gamma equal to 1.
By manipulating the slide control using the mouse, you may not be able to
set gamma exactly to 1. In this case, use the [arrow-up] and [arrow-down]
keys to change the slide control in steps of 0.01.
The Saturation slide control enables you to alter the color rendering from a
black and white signal to maximum color saturation. In most cases, the
"good" setting of this value is seldom higher than the range of 0,10 to 0,20.
The Gamma slide control's value range is from 0,00 to 2,00; the Saturation
slide control's range is from 0,00 to 0,99.
Click the Default button to use the default values defined in the Define Default Color Settings dialog box from now on.
Click the Define... button to set the standard color settings. The Define Default Color Settings dialog box is opened.
Camera Control
Factory settings
Current Settings
Click the Factory settings button to return the color settings to their original
levels. The factory settings have been adapted in such a way that the color
rendering with a specific equipment configuration is ideal for a large scope
without any additional changes having to be made.
Click the Current settings button to make the current values be the future
standard.
Sharpen filter
Sharpen Filter
On/Off
Sharpen Filter
Settings
Use the sharpen filter to increase the sharpness using edge enhancement
already with the live acquisition.
Click the Sharpen Filter On/Off button to activate/deactivate the sharpen filter. The manner in which the acquired image is affected by the sharpen filter
depends on the sharpen filter parameter. You can adjust this parameter by
using the Sharpen Filter Settings dialog box.
Clicking the Sharpen Filter On/Off button produces the same results as selecting/clearing the Activate check box in the Sharpen filter group in the Input tab. The button is simply a more convenient alternative.
For more information about the sharpen filter and sharpen filter parameter
have a look at the chapter "The Input tab S. 22"
Click on the Sharpen Filter Settings button to open a dialog box where you
can set the sharpen filter parameter:
9
Available
The Sharpen Filter Settings button is only available if the sharpen filter has
been activated.
To adjust the numerical value of the sharpen filter parameter, use the mouse
to move the slide control to the right or left. This is a convenient alternative
to using the Input tab - where you can also adjust this numerical value (in
the Sharpen field).
CC-12 • Camera Control
Automatic Gain Display and Scaling
No Settings
Fixed scaling
Automatic gain display
Use automatic
gain display
10
Histogram calculation on full
image
Histogram calculation on
crosshair
Histogram calculation on ROI
Set ROI for histogram
Use fixed scaling
Automatically
adjust fixed
scaling
The CC-12 uses a dynamic range of 3 x 12 bit. However, only 3 bits x 8 bits
can be used for the display and additional editing of images. Therefore, the
data transferred from the camera to the computer must be adjusted directly
during acquisition so that this condition is fulfilled. There are different methods for this purpose which can be utilized based on the specimen being observed as well as the microscope and camera settings.
The four lowest bits are ignored should you not make any settings. Thus, at
large, only imperceptible color alterations to individual pixels occur.
The "Fixed scaling" method gives you a fixed defined intensity area to be
used for each channel. To do this, set the limits in the range from 0-4095. All
signals which are outside the defined areas will be ignored. It is not possible
to set a bottom limit with color cameras due to the fact that this would lead
to strong color alterations. Use this method with images with weak contrasts
or to select a specific signal area (fluorescent microscopy).
The "Automatic gain display" method extracts the area to be used based on
the actual signals on hand. The strongest of these signals will be ignored.
Use this method with images with a high dynamics range or low maximum
signal strength.
Click the Use automatic gain display button to activate the automatic gain
display. The live-image, independent from the illumination conditions, is always shown in optimal contrast. The behavior of the "automatic gain display" depends on whether or not this function's desired overflow has been
set and on which area of the image the contrast enhancement is going to
take place. The desired overflow can be set using the Image > Configure Input command located on the Display tab. The following possibilities for selecting the image area can be found in the Camera Control dialog box:
Click the Histogram calculation on full image button to carry out the contrast
optimization on the basis of the entire image. This option is recommended if
the structures to be observed are equally dispersed throughout the entire
image.
Click the Histogram calculation on crosshair button to only consider the pixels along a strip which is only a few pixels wide in the horizontal and vertical
direction.
Click the Histogram calculation on full image button to carry out the contrast
optimization on the basis of a rectangular area within the image. You can
use this option if relevant details are visible in a part of the image. The display can thus be optimized for this area.
Click the Set ROI for histogram button to interactively set the rectangular
area of the image for optimizing the contrast. Pull the ROI's frame shown in
the image document to the desired position and set its size by moving the
mouse and keeping the right mouse button depressed. Rightclick to confirm
position and size of the ROI.
Click the Use fixed scaling button to use a fixed scaling amplification. In contrast to the automatic gain display, the fixed scaling utilizes a predefined signal range which is manipulated so that it uses the entire dynamic area.
When using the fixed scaling, the image is no longer displayed optimally if
the illumination or sample are altered.
Click the Automatically adjust fixed scaling button to automatically set the
scaling on the current conditions. This button is only available during an active live acquisition.
Camera Control
Manually adjust fixed scaling
Click the Manually adjust fixed scaling button to manually set the upper right
hand limit for the fixed scaling. The Fixed scaling dialog box is opened.
Move the Right limit slide control or directly enter the desired numerical value into the text box.
Black balance
Functionality
How to...
The black balance is used for background correction. The program calculates the mean value for each color channel in an area (ROI) which you defined. These values are subtracted from all pixels in the entire image. The
black balance is to be used predominately for fluorescence acquisitions in
order to balance the undesired background in the acquisition. A discoloration of the images occurs if the black balance is used with brightfield acquisitions.
The black balance is carried out based on the rectangular area in the image,
in which a uniformly dark-gray to black signal is to be located. The mean signal value is determined for each color channel within this area. This value is
reduced from each pixel when applying the black balance.
This is how you carry out a black balance:
Sharpness Monitor
The Sharpness Monitor allows you to control the focus settings during a live
acquisition. You are also able to define a rectangular area of the image
which is to be analyzed for the sharpness monitor in the same way as for the
11
1) Take a snapshot which contains a suitable area to be used for black
balance.
2) Use the Camera Control command.
3) Click the Set ROI for Black Balance button to adduct an area of the image for the black balance.
4) Pull the ROI's red frame shown in the image document to a position
which contains an area which is as uniformly dark as possible.
" Change the size of the area by keeping the mouse depressed and
moving the mouse. The ROI used for the black balance cannot be
made to any large size possible.
5) Rightclick to confirm position and size of the ROI.
" The values are calculated for the black balance on the area defined
in such a way.
6) Click the Black Balance On/Off button to activate the black balance.
" The correction values calculated for the black balance are deducted from all the pixels in the entire image.
The black balance function is available for live acquisitions as well as for the
acquired snapshots. A ROI for black balance has to be set before performing the black balance on single images. The Black Balance button for acquired images is part of the Black button bar.
CC-12 • Camera Control
Meaning of the Sharpness Monitor
Focussing and Sharpness Monitor
Reset to 90%
12
How to...
white balance and black balance. The maximum contrast serves as a monitor for the sharpness in this area. Therefore this area should be limited to
an image segment in which the contrast is a good monitor for the sharpness.
The Sharpness Monitor consists of a dialog box in which a relative measurement of the sharpness is displayed by a changing bar which can be varied
between Blurred and Focused.
If you start a live acquisition with an activated Sharpness Monitor or activate
it during a live acquisition, it will show middle sharpness (50%) in each case.
This is because there is no absolute measurement for the sharpness in an
image and because the sharpness monitor only gives relative values which
are related to the initial situation which was present when starting the live
acquisition.
The length of the bar changes if you alter the focus settings during a live acquisition in progress with an active sharpness monitor. In doing so, the
green markation bar shows the maximum sharpness reached since the live
acquisition was started. The bar shifts accordingly to the right should the
sharpness increase due to your changes. If the focus settings are already
optimal when starting acquisition, the bar can only shift in direction Blurred.
Click the Reset to 90%button to set the sharpness monitor's relative value
to 90%. Utilize this function if you start working with a very blurry image and
if the sharpness monitor's bar reaches the limit to the right while focusing.
By resetting to 90%, the display gets more room for additional focussing.
The Reset to 90% function is also available if the sharpness drops very drastically due to changes made to the microscope settings (i.e. changing objectives) during an acquisition in progress.
This is how you set the sharpness monitor for good acquisitions:
1) Set all other parameters (microscope settings, exposure time, white
balance) optimally. The application of the sharpness monitor should be
the last step before image acquisition.
2) Acquire a snapshot.
3) Use the Camera Control command.
4) Click the Set ROI for sharpness monitor button to interactively define a
rectangular area of the image for the sharpness monitor.
" To do this, select an area of the specimen for which a good sharpness setting is especially important.
5) Begin a live acquisition.
" The Set ROI for sharpness monitor button becomes inactive.
6) Click the sharpness monitor on/off button to open the sharpness monitor dialog box.
" The sharpness monitor begins with a value of 50%.
7) Vary the focus settings and observe the sharpness monitor while doing
so.
" The sharpness monitor bar shows the current sharpness, the green
markation shows the highest achieved sharpness.
" Mind the exposure time when setting the focus. The sharpness
monitor alters itself with a certain amount of delay when using larg-
Snapshot
er exposure times (> 100ms). Vary the focus settings in small intervals and wait each time until the sharpness monitor has been
adapted as well.
8) Find the focus settings by which the sharpness monitor is at its maximum and use these settings for the acquisition.
Snapshot
Use the Image>Snapshot command acquire a single images and to make it available for the image-analysis software.
You may also
Available
Before acquiring an image
You can also click the Standard (button bar) > Snapshot button.
The command is not available if the active image buffer is write protected.
If the live mode was enabled with the Image > Acquire command, it will
automatically disabled with the Snapshot command.
You should first check the live mode if you are not certain if the object to be
acquired is positioned, focussed, or illuminated correctly. The illumination of
the object, the setting of the diaphragm, as well as the setting of brightness
and contrast are important for the quality of your images.
intelligent Exposure
Use the intelligent Exposure procedure to make acquisitions with little effort.
Histogram-Analysis
Related Topics
Use automatic gain display 10
Dynamic limit in % 15
13
Calibration
intelligent Exposure largely makes the acquisition procedure automatic. The
exposure times for the live acquisition and the snapshot are automatically
set independent of one another so that the dynamic range of the camera are
fully taken advantage of. The optimization of the exposure time occurs continually and automatically.
intelligent Exposure analyzes the real time histogram during a live acquisition in order to optimize the exposure times.The automatic gain display and
the exposure time are set so that the signals use one of the dynamic ranges
set by the user.
intelligent Exposure arranges linear correlation between the exposure time
and the mean gray value for calculating the optimal exposure time. this requires the dark current of the camera to be known. Thus, the first step to
working with intelligent Exposure is measuring this dark current via the acquisition of an image using a darkened camera. When first starting intelligent
Exposure, you will be automatically asked to carry out this calibration.
CC-12 • intelligent Exposure
intelligent Exposure
14
Exposure times
Acquisition
Snapshot
Note
Exposure time correction
This calibration step measures the camera's dark current, in order to make
an offset correction possible. After measuring the dark current, you may
make acquisitions with intelligent Exposure.
You must execute the calibration anew should you change cameras, i.e. by
switching to another input channel. You have access to this function by using the Calibration... button located in the intelligent Exposure > Options dialog box which is described further down.
Click the intelligent Exposure button to be able to make acquisitions using
this method. The intelligent Exposure dialog box is opened.
The exposure times ascertained by intelligent Exposure are displayed in the
dialog box's status bar. The term Live Exp. (Live Exposure Time) stands for
the exposure time of the live acquisition, while Snap Exp. (Snapshot Exposure Time) stands for the exposure time of the snapshot. Independent of the
calculated exposure time for the snapshot, the exposure time for the live acquisition cannot exceed 125 ms. In doing so, a quick live-image is guaranteed which simplifies the microscope's settings when the camera is running.
It is possible that the live-image is very dark, should the changes you made
to the setting result in very long exposure times for snapshots. Please alter
the microscope settings accordingly in such cases.
Click the Acquire button to start the live acquisition using intelligent Exposure. The live-image is displayed in the image document. You can make the
necessary settings (focus, stage settings) with the help of this live-image.
Click the Snapshot button to initiate the acquisition of a snapshot. intelligent
Exposure acquires an image and saves it to the active image buffer. A hint
box informing you about the acquisition process appears if the exposure
time of a snapshot exceeds one second.
Use the Special > Preferences... command to be able to acquire numerous
snapshots simultaneously without overwriting them. Enable the Images tab.
Select the Image buffers (All) entry from the. Image acquisition> sequence
list. A new image buffer is automatically enabled after the acquisition of a
snapshot.
The Exposure time correction slide control enables you to manually influence the exposure time for snapshots calculated by intelligent Exposure.
Position ’0’ on the slide control does not influence the exposure time which
was automatically calculated by intelligent Exposure. Move the Exposure
time correction control to the right in order to increase the exposure time, or
to the left to shorten it. The exposure times for live acquisitions and snap-
intelligent Exposure
Note
Resetting the Exposure
time correction
Exposure Time
Lock
Preview
White Balance
Options
shots are equally altered during correction, as long as the exposure time for
snapshots is lower than 125 ms. The exposure time for the live acquisition
remains unchanged for exposure times exceeding 125 ms.
Moving the control to the left only leads to a hardly visible change in brightness in the live-image. This is due to the automatic gain display function
which is always enabled when using intelligent Exposure.
Double click on ’0’ below the Exposure time correction slide control in order
to use the automatically calculated exposure time values once again.
Click the Exposure Lock button to lock the current exposure time for intelligent Exposure. As of now, no more alterations will be made to the exposure
time. Click the button anew to return to the dynamic adjustment of the exposure time.
Click the Preview button to use the setting for snapshots with live-images as
well. Now the resolution and exposure time for snapshots will be used. Click
the Preview button anew to once again use the settings for the live acquisition.
Click the White Balance button to carry out a special white balance when using intelligent Exposure. In the image document, move the ROI's red rectangle to a white or uniformly gray position on the specimen. Change the size
of the ROI by keeping the right mouse key depressed and moving the
mouse. Rightclick to confirm position and size of the ROI.
Click the Options button to set the options for intelligent Exposure. The intelligent Exposure dialog box is opened.
15
Dynamic limit in %
Note
Max. exposure time in
s
Set the dynamic range in which intelligent Exposure works with the Dynamic
limit in % slide control. Move the controller to the right to increase the dynamic limit. This setting results in lighter images with a higher contrast in
darker areas of the image. Move the controller to the left to decrease the dynamic limit. This setting results in darker images with a possible better contrast in light areas of the image. This effect is not distinctly evident when using color mode.
Due to the calculation procedure, setting the Dynamic limit in % to 90 can
lead to an overflow. You should reduce the Dynamic limit in % for acquisitions whose high gray values contain important information for the image, for
example in fluorescent microscopy.
Set the highest exposure time to be used for snapshots using the Max. exposure in s slide control. You can also enter the desired text directly into the
text field. This value determines the maximum exposure time for snapshots
which intelligent Exposure can use. The maximum value which can be set
CC-12 • NetCam
Max. exposure preview
in s
Keep live after snapshot
Calibration...
here depends on the camera being utilized. A warning occurs and the pictures become darker should intelligent Exposure calculate a higher exposure time than determined by Max. Exposure in s.
Set the highest exposure time to be used for live image acquisitions using
the Max. exposure preview in s slide control. You can also enter the desired
text directly into the text field. This value defines the maximum exposure
time for live image acquisitions which intelligent Exposure can use. The
maximum value which can be entered here, depends on the camera being
utilized. A warning will appear should this value be exceeded by the current
illumination conditions.
Select the Keep live after snapshot check box, in order to continue the live
acquisition directly after a snapshot. A snapshot is created in the live acquisition's image buffer and a new live acquisition is started in the next image
buffer defined in the sequence. Keep live after snapshot is only available if
you have selected an entry other than None in the Sequence list located in
the Special > Preferences > Image > Image acquisition dialog box.
Click the Calibration... button to begin the camera's calibration process. The
procedures for calibration are the same as the procedures located in the
section entitled "Calibration".
NetCam
16
Transferred images
Definition
Related Topics
Calibration 13
You can transfer the live-image via the intranet or internet to other computers using the add-In NetCam.
This enables you make the images available for your colleagues directly
during acquisition. Thus, interactive work over large distances is made possible using simultaneous communication, e.g., telephone. The images are
compressed so that they can be transferred quicker.
The live-image is directly transferred. Based on the speed of your network
connections and the attained refresh rate during acquisition, you will get the
impression of a live-image. In an internal company network, a direct backup
coupling from observer to user is possible without any elapsed time.
NetCam can also transfer a fixed image, whereby in this case the image's
overlay is also transferred.
The Server is the computer on which both the image-analysis software and
the NetCam are installed. The image acquisition is controlled from the server.
The Client is each computer which can access the server's images via internet. The client cannot control image acquisition.
Installing the web server
Available
A web server must be installed on your computer to make images available
via the internet. An appropriate program is automatically installed by Setup,
if you have selected the add-In NetCam. This step will be skipped should a
web server already exist on your computer.
Warning
When using a computer connected to a network it is possible that the security settings will not allow the use as a web server. Therefore, contact your
system administrator.
In the Module\NetCam subdirectory of your image analysis program, you will
find the file "NetCam_Info.pdf". This file contains technical explanations
about NetCam which you can give to your system administrator.
Installing the web server
The web server required for the use of NetCam is automatically installed during the setup.
The installation of the web server is started directly by setup. After having
agreed to the license terms, you can make further settings in a dialog box.
17
Complete, Custom
You can simply adopt the default settings.
We recommend the complete installation of the web server.
NetCam in the intranet
Enabling access
The transfer of images within the company network or intranet generally
does not require any further actions.
During installation, setup will create a link with the name NetCam.html in the
\Module\NetCam subdirectory below the working directory of your imageanalysis software. Send this link via e-mail to colleagues you would like to
give access to your images. The easiest way to do this is with the following
command: Send to > E-mail receiver in the context menu of the link in Windows Explorer.
CC-12 • NetCam in the internet
Java
Java Runtime Environment, Version 1.4.1_03 or higher is required to show
the images on the clients. When this requirement has not been fulfilled by
the client, the transfer of images cannot be carried out.
NetCam in the internet
Adapting the security
settings
IP Address
Determining the IP address
How to...
When using NetCam, a direct communication takes place between the server and the client. If the communication goes beyond the company network,
you must, if need be, make changes to the security settings.
The adaptation of a network's security settings takes place via the respective system administrator. The necessary technical and security requirements for the use of NetCam are outlined in a separate file entitled
"NetCam_Info.pdf" which was saved by setup to your system's image-analysis software \Module\NetCam subdirectory. Please give this file to your
system administrator so that he can make the appropriate adaptations.
The server's internet address must be entered on the client's page. This address is available in the well-known alphanumerical form ("MyComputer.OurCompany.de") and in the numerical form ("62.180.61.137"). The numerical form of the address is more reliable, especially if your system administrator has to alter the network settings.
You can find out your computer's IP address from your system administrator
or determine it yourself.
This is how you determine your computer's IP address:
18
1) Select the operating system command Start > (All) Programs > Accessories > Command Prompt.
" The console box is opened.
2) Enter the ipconfig command with your keyboard and use the enter key.
" Data about your computer's network connection are shown in the
console box.
Java
3) Note the entry in the IP address row (192.168.118.175 in example
above).
4) Make this information available to the users on the side of the clients.
Java Runtime Environment, Version 1.4.1_03 or higher is required to show
the images on the clients. When this requirement has not been fulfilled by
the client, the transfer of images cannot be carried out.
Using NetCam
Using NetCam
The use of your image-analysis software remains the same when using NetCam. You can use all of the functions as always. To transfer the images to
other users and to be able to control the transfer, use the buttons located in
the NetCam button bar.
Click the NetCam On/Off button to start or end the transfer of images with
NetCam.
The transfer of live-images begins as soon as NetCam is activated. The
transfer continues until you deactivate NetCam or close your image-analysis
software.
Click the NetCam Settings button to set image options and to administer the
clients which are to have access to your images. The NetCam Settings dialog box is opened.
NetCam on/off
NetCam Settings
Connections
Connected Clients
>>
Banned Clients
Add new...
19
Disconnect
Use the Connections tab to administer the clients which are to access your
images.
In the Connected Clients list you will see all of the clients which are currently
connected with your computer. You can mark one of these clients to disconnect the connection to it or to refuse a connection in the future.
Click the Disconnect button to disconnect the connection to the marked client. The client can once again make a connection.
Click the >> button to include the marked client in the Banned Clients lists.
The corresponding client is automatically disconnected.
In the Banned Clients list you will see all of the clients who you have forbidden access to your image-analysis software. From these computers it is impossible to load and view the images made available by NetCam.
Click the Add new... button to add computers or entire groups of computers
to the Banned Clients list. The Enter IP(s) or a domain dialog box is opened.
CC-12 • Using NetCam
Domain name
Procedure
IP Address(es)
20
From, to
Enter the alphanumerical name of a domain into the Domain name field if
you want to block the access of computers within this domain. For example,
enter the domain name foobar.com to hinder the transfer of the images to all
the computers within the domain foobar.com.
NetCam sends a query to the nameserver to get the numerical IP address
belonging to the domain provided. You will receive a message, should the
query lead to an error. The provided domain will not be placed on the
Banned Clients list.
You can provide a numerical IP address or an entire address range in the IP
adresse(s) group to exclude the computer in question.
Enter the limit of the address range to be excluded in the from and to fields.
Image options
Image quality
Image quality and data
transfer rate
In the Image Options tab you can set the quality of the transferred images
and thus influence the transfer rate.
Use the Image Quality slide control to control the compression of the images. Move the slide control to the right to maintain a high image quality. The
data amount of the transferred images increases, whereby the transfer rate
decreases. Move the slide control to the left to increase the compression.
The image quality decreases, whereby the transfer rate increases.
A single CC-12 image can be up to 4 MByte in size. Images of this size take
a long time to transfer to a client even with a very fast network connection;
the live-image effect is thus lost. Therefore the images are compressed for
transfer. The used algorithm is equivalent to that used with JPEG images.
Thus, high compression rates are achieved with decreased image quality.
The amount of compression achieved is based on the image type. Images
with large, unison colored areas can be compressed more than detailed images without these characteristics.
Camera Set Up
Camera Set Up
Status after installation
An input channel is automatically created when installing the software, immediately enabling you to make image acquisitions. This channel is, in many
cases, adequate for all demands.
Apart from the actual installation, the configuration of one or more input
channels is also part of the installation required for setting up the camera.
Software Camera Control
After successful installation, you can configure the camera for using the image-analysis software.
Logical input channels
Configuration via tabs
Set Input
The CC-12 software complies with the logical input channel concept. This
means the camera is as easy to operate as a regular video camera if it is
hooked up to the microscope and computer, and if the software has been
installed and configured. No additional menu appears in the user interface's
menu bar. When you have selected the Set input... command located in the
Image menu, you will realize that you have additional logical input channels
at your disposal which can be set up to fit your needs.
Channels are configured via the inputting of suitable values in various tabs.
The 3 tabs relevant for CC-12 will be dealt with in-depth.
Logical input channels contain, for example, the information about how large
an image format is, and what is to happen with the image signal before it
reaches the image buffer. Each channel must be calibrated separately. A
channel for image acquisition via CC-12 is usually called "CC-12 FW...".
Use the Image > Set Input... command to select a new input channel or to
create a new one. You can also depress the [F6] key. The Set Input dialog
box is opened.
The Set Input dialog box
with an already defined input channel.
21
New Channel
Click the New Channel button to create an entirely new input channel. The
Select device dialog box is opened. All connected image creating devices
are listed in the Available devices list.
CC-12 • Software Camera Control
Select Device dialog box
with a connected CC-12
camera.-12
The serial number of the
camera is quoted to allow
the distinction of more than
one camera connected at
the same time.
Creating additional input
channels
Duplicate
Channels
Configure Input
22
The Input tab
Select the desired camera from the list Available devices and click on the
OK button to create a new channel.
Should you want to create additional input channels, for example to have different configurations immediately available for repeating tasks, use an already existing input channel.
Click the Duplicate Channel button to make a copy of the active input channel. The new input channel gets the same name as the channel from which
it was copied and receives a sequential number. The newly created channel
is automatically enabled.
Click the Configure Input button to adapt the settings for the new channel.
The Configure Input dialog box is opened. You can also open the dialog box
using the Image > Configure Input command or by double clicking the camera name located in the status bar.
Software Camera Control
Exposure
Sharpen filter
Activate
Sharpen
Mirror
Vertical
Info
Related Topics
Camera Control 6
23
Horizontal
Enter the number of microseconds the CCD chip of the camera is to be exposed into the Exposure field. You can also use the arrow key (to the right
of the field) to select this value. The chapter entitled "Camera Control" explains how you can change the value in this field with the help of the Camera
Control... command interactively while viewing a live-image.
The smallest possible exposure time is 100 µs and the largest is 160 s. The
dynamic breadth from ultra-short to long-period exposure enables the acquisition of high-intensity, brightly exposed images, as well as the fluorescent
microscopic reproduction of very dim glowing structures.
Select the Activate check box of the Sharpen filter group to enhance the image sharpness. This sharpen filtering takes place in real time during image
acquisition. When applying the sharpen filter, the numerical value in the
Sharpen field determines whether or not the image’s contours appear sharper or softer.
Raising sharpness will accentuate edges, but also brings out image noise
more.
Please note that when you use the sharpen filter you not only alter the monitor display, but the actual image information as well. This means that when
you are planning on quantitatively evaluating your images later, you should
not use the sharpen filter. The Oper menu provides you with commands you
can use at any later time for adjusting an image’s sharpness.
Enter the desired sharpness parameter into the Sharpen field. You can also
use the arrow key (to the right of the field) to select this value. Values ranging from -30 to 30 are possible, whereby the default value is 10. The image
appears smoother when using negative values and sharper when using positive values. Entering a value of 0 will result in the sharpen filter having no
effect at all.
Convenient alternatives for activating/deactivating the sharpen filter, as well
as for adjusting sharpen filter parameters, are the Sharpen Filter On/Off button and the Sharpen Filter Settings button - both in the Camera Control dialog box.
The Mirror group enables you to determine if the camera's image is to be
mirrored directly into the program during transfer. Both mirror functions can
be combined with each other.
Select the Horizontal check box to mirror the image horizontally during acquisition.
Select the Vertical check box to mirror the image vertically during acquisition.
Click the Info... button to have some device data, as well as the current temperature of the CCD chip and the camera housing shown in the CC-12 Information window:
CC-12 • Software Camera Control
Device Name
Devices ID
Firmware Version
Serial Number
Camera Type
DLL Version
SYS Version
Temperature
24
CCD Chip
Housing
The Device Name relates to the inner construction of the camera and is
specified by the manufacturer.
The Device ID is the physical identification of the camera for the operating
system. It is in accordance with the device's serial number.
The Firmware is the cameras software for your operation.
The serial number serves as a clear identification for each camera produced.
The Camera Type is the exact identification for the camera model.
The DLL Version is the version number of the camera's driver.
The SYS Version is the version number of the camera's device driver. DLL
and SYS Version are identical.
The cameras are equipped with temperature sensors. The temperatures are
continually measured.
CCD Chip is the CCD chip's temperature. An increase in noise is the result
of high temperatures of the CCD Chip. The default value for trouble-free operation is 10°C.
Housing is the temperature of the cameras housing. The cameras housing
should not exceed 40°C.
Software Camera Control
The Display tab
Over exposure
Display warning
The Over exposure group enables you to determine if and when you should
be warned of an overexposure. The software continually checks the image
intensity in live mode, by analyzing the image's gray-value histogram in real
time. You will be warned if the live-image is overexposed. The warning will
also appear following acquisition of an overexposed single image.
Select the Display warning check box to have a warning message shown if
the image is being overexposed:
25
Overflow
Automatic gain display
In this case, reduce the exposure time till the warning message disappears.
An image is overexposed when a certain percentage of its pixels attain the
maximum gray value possible. This percentage is entered into the Overflow
field.
Use the functions in the Automatic gain display group to have the image always displayed optimally onscreen, no matter what the actual lighting conditions are. The system assesses the current gray-value histogram - in real
time - in order to obtain optimal display of the live-image on the monitor.
Note that the automatic gain display not only effects the monitor display, but
also effects the image information. If it is activated, the bit-depth of the acquired images is reduced from 3 x 12 Bit to 3 x 8 Bit with the help of the optimized lookup table. If automatic gain display is not activated, the 4 lower
bits (from the 12 bits of each component color) will simply be clipped.
CC-12 • Software Camera Control
Activate
Left overflow
Right overflow
Fixed scaling
Application
Activate
26
Left limit
Right limit
Online histogram
Select the Activate check box to enable the automatic gain display. The effects of this check box is the same as clicking the Use automatic gain display
button located in the Camera Control dialog box (<S. 10). The Activate
check box is not available if the check box with the same name in the Fixed
Scaling group is enabled.
When the automatic gain display is active, image intensity for the image on
the monitor will be spread between the gray values Gmin and Gmax. You determine the values of these Gmin and Gmax gray values in the Left and Right
overflow fields: this is where you enter the percentage of pixels with the lowest gray values which are to be displayed black, and which percentage of
the highest gray values are to be displayed white.
This is how you clip gray values that are to be ignored at both the upper and
lower ends of the gray-value histogram. Make use of the overflow values so
that individual pixels or minute image areas that are too bright or too dark do
not influence the automatic gain display.
Instead of using the Automatic gain display group, you can use the Fixed
scaling group. The group whose Activate check box has been selected will
be the one available - the other will not be available.
The functions of the Fixed scaling group are for manually influencing onscreen image display. You select definite limits - Gmin and Gmax - for the
gray-value range that you wish to have optimally spread for onscreen display. Any gray values occurring outside this defined gray-value range will
appear either black or white.
This is how you can, for example, improve the visibility of the specimen
structures of interest to you - by having dark areas of the specimen displayed entirely in black.
Please note that this manual presetting of the gray value, like the automatic
gain display, not only effects the monitor display but also the image information.
Select the Activate check box to switch on the manual definition of the grayvalue range to be displayed.
Setting the left limit is not possible for the CC-12 camera, as this would lead
to a color tinge.
Enter the value of the right limit for the fixed scaling into the Right limit box.
Its function is equivalent to the Right limit parameter, which you can influence by clicking the Manually adjust fixed scaling button located in the camera controls dialog box.
Select the Online histogram check box to be able to keep an eye on the
gray-value distribution during image acquisition. During image acquisition, a
window showing the current histogram will appear automatically. This histogram will be continually updated.
Related Topics
Manually adjust fixed scaling 11
Software Camera Control
Setting the fixed scaling
via the online histogram
Live overlay
Underscan
Overscan
Related Topics
Use automatic gain display 10
Use fixed scaling 10
27
Image scaling
Minimum, mean, and maximum gray values of the image will be shown beneath the histogram. Percentages are in relation to the maximum obtainable
gray value. The maximum obtainable gray value when using CC-12 (with its
-12 12-bit depth) is 4095. This gray value is equivalent to 100%. A mean value of 53,1% when operating in black & white is equivalent to a gray value of
2174.
During a live acquisition, you can turn on or off the Automatic Gain Display
with the buttons in the online histogram, as well as activate and set the fixed
scaling.
You use the mouse to set the limits of the fixed scaling in the online histogram. Move the pointer over the blue (lower limit) or red (upper limit) vertical
line in the histogram. The pointer turns into a horizontal double arrowhead.
Move the mouse with the mouse button depressed to the left or right to either
increase or decrease the limit values. The changes can be viewed directly
in the live-image.
Select the Live overlay check box if you want to use overlays in live-images.
Live Overlays are mandatory for the white balance functionality.
An image’s overlay contains information in the form of text, markers, or other
graphical elements. The overlay can be displayed together with the corresponding image without the actual image information being affected.
The Live overlay check box is only available if your display board features a
so-called hardware overlay which is supported by Microsoft DirectX. Almost
any up-to-date AGP display board provides you with that feature. You can
look at the System Info dialog box to check whether your display board offers this feature. To do so, select the Info... command from the ? menu and
click on the System Info... button.The System Info dialog box will be opened.
Click on the "DirectX" entry in the list on the left-hand side to have the desired information appear in the list on the right-hand side.
Select one of the four selections for displaying an acquired image in a Viewport in the Image scaling list.
Select the Underscan entry to have the whole image displayed. The system
will select the largest zoom level - 25%, 50% or 100% - where the whole image can still be displayed within the Viewport. In certain cases not all of the
Viewport’s available area will be used.
Select the Overscan entry to ensure that all the Viewport’s available area will
be used. The system will select the smallest zoom level - 25%, 50% or 100%
- where the image fills the whole Viewport. In certain cases parts of the image will not be visible.
CC-12 • Software Camera Control
Adjust to viewport
Full size (100%)
Histogram calculation
Full image
Crosshairs
ROI
28
Application
Select the Adjust to viewport entry to have the image size adjusted to optimally fit the Viewport. The image will be adjusted to fit the current size of the
Viewport.
Select the Full size (100%) entry to have the image displayed without any
zooming. Only the left upper corner of the image will be shown if the Viewport is smaller than the image.
Select the pixels to be taken into consideration for calculation of the grayvalue histogram in the Histogram calculation list. There are three options:
If you select the Full image entry, the gray values of all pixels will be applied
to histogram calculation. This is the most commonly used setting when using light microscopy.
If you select the Crosshair entry, only pixels of a particular image area are
used in the calculation. This image area is shaped like cross hairs that are
centrally positioned on the image. The vertical and horizontal segments of
these cross hairs are respectively just a few pixels wide and cover just about
the entire height and width of this image. Crosshair is the default setting
when using transmission electron microscopy.
Select the ROI entry to carry out the optimization of contrast and exposure
time based on a rectangular frame.
These settings effect all real time functions which use the gray-value histogram: over exposure warning, Automatic gain display, and the Online-Histogram.
We recommend selecting Crosshair if you wish to speed up histogram calculation. You can speed up the frame rate of the live-image depending on
what real time functions you have activated. You should however make sure
that the pixel gray values around the middle image line and around the middle image column are representative for the gray values of the entire image.
If this is the case, you will not notice any difference compared to the Full image option for the over exposure warning, Automatic gain display, and the
online histogram.
Software Camera Control
The Format tab
Active area
Image format
Binning
Clipping border (pixel)
Left, Right, Top, Bottom
29
Horizontal, Vertical
The display in the Active area field shows how large the acquired image is
using the present settings with regards to the largest possible image size. If
you make changes to the clipping, you can directly view their effect here on
the active area.
You can choose a means of image acquisition from the Image format list.
The entries in this list correspond to predetermined settings for clipping and
binning. In addition to the predetermined list elements, you can also select
from the settings which you defined.
The CC-12 offers binning to reach a higher sensitivity. With binning the signals form several pixels are summed up into one pixel. This summing up results in greater signal strength, but at the coast of diminished resolution. Select a binning factor in the Binning group. All images acquired with binning
are grayscale images. For a gamma value other than 1, the adaptation of
gamma is handled by applying a gray-LUT. Therefore, the image is advertised as false-color image in the image manager.
The binning factor is the same in horizontal as in vertical orientation. Set the
desired binning factor (1, 2, or 4)in the Horizontal or Vertical field. The factor
in the other field will automatically be adapted. You can also activate binning
by selecting an appropriate entry from the Image format list.
When clipping is used, the edges of the images are clipped. Hence, clipping
does not have any effects on the resolution and brightness as well as the
required exposure time.
The fields entitled Left, Right, Top and Bottom show the amount of pixels
that are to be clipped from each image edge. Each individual value can
reach from 0 (no clipping) to almost the entire image in each respective direction (complete clipping). If a value for the clipping already has been set,
the setting possibilities for clipping the opposite edge reduce themselves respectively.
CC-12 • Software Camera Control
Interactive
30
No Clipping
Click the Interactive button to be able to place the clipping interactively into
the image document. Move the pointer with the mouse button depressed to
change the size of the area to be acquired. The actual acquired area is
marked by a red frame. Move the mouse to alter the position of the marked
area within the image document. Rightclick once to fix the area to be acquired.
Click the No Clipping button to return the values for Left, Right, Top and Bottom back to 0, thus disabling Clipping.
Appendix I: Microscope Settings
Appendix I: Microscope Settings
Your digital camera can only achieve high image quality if the microscope
has been optimally set. A subsequent correction to the software can never
correct deficiencies in the image resulting from faulty settings made to the
camera and microscope.
The optical system microscope offers numerous setting possibilities. In order to attain best acquisition results, the settings must be made precisely.
In spite of varying models and observation possibilities, the microscope's
image producing components are in accordance with each other.
A microscope basically consists of the optical components: projection objective and magnifier. The projection objective is the objective with which the
observation is to be carried out. The eyepiece takes on the function of the
magnifying glass. An additionally important component is the illuminator.
This illustration clarifies the
basic assembly of the components of a microscope in
transmitted-light mode.
31
Illumination
Light is a decisive medium for creating images with a microscope. As a result, the light, i.e. illumination,
plays an important role in creating the image in the microscope.
Lamp Selection
Tungsten-halogen
lamps
The goal of the illumination settings is the equal distribution of light on the
sample with parallel light rays falling on the object. The illumination must be
bright enough to make all of the image details visible; however over-illumination leads to images being too bright and low-contrast. In addition, the color of the light has a strong impact on the sample's color rendering.
The tungsten-halogen lamps and gas discharge lamps are the most commonly used lamps in microscopy. The light creating processes of both lamp
types are fundamentally different.
Tungsten-halogen lamps comply with their functionality to a conventional
light-bulb. Electricity flowing through a metal wire heats the wire to the extent
that light is produced (thermal emitter). In doing so, the wire emits a continuous spectrum, hence a broad spectral band is emitted. One can control the
CC-12 • Illumination
Gas discharge lamp
ND-filter
brightness as well as the color (color temperature) of the lamp by controlling
the electrical current. Tungsten-halogen lamps can be widely used in microscopy, especially when acquiring color images.
Gas discharge lamps stimulate gas at the atomic level using strong electrical
fields. The charged atoms return to their initial state and, in doing so, give
off the energy which initially charged them in the form of electromagnetic radiation: light. The resulting photons each have characteristic energy which
is typical for the gas being used. As a result, this type of lamp does not emit
a broadband spectrum like the halogen lamp, but instead emits discrete
wavelengths. This fundamental physical process cannot be externally influenced; hence, such a lamp's perceived color is independent of the operation
conditions. Because of this discrete spectrum, gas discharge lamps do not
emit a color-neutral illumination. Its adjustable high intensity light and the
emission of short-wave spectral bands required for fluorescent microscopy
is an advantage.
Many microscopes can insert ND-filters into the light path, thus reducing the
intensity of the lamp's light. These ND-filters ensure a uniform reduction of
light intensity throughout the entire frequency range without altering the color temperature.
Köhler Illumination
Condenser
32
Note
Principle of Köhler Illumination
Focussing the Field Diaphragm
Adjusting the Condenser
This illumination process which was described by Dr. August Köhler as early
as 1893, is still an indispensable prerequisite for successful analysis using
light microscopy. The Köhler illumination enables an optimal, homogenous
illumination of solely the part of the sample to be analyzed.
The condenser carries the light onto the sample. The main components are
the aperture stop with which the size of the illuminated area is set and the
condenser lens which is responsible for the parallel emitted light rays. The
condenser can be opened and closed. The condenser is height-adjustable
and horizontally adjustable.
Set the aperture stop to 2/3 of the entire opening and do not use it to reduce
the brightness. To do that, you should always use ND-filters or a lower lamp
voltage.
You will find comprehensive descriptions about the principle of Köhler illumination in microscopy text books. We will only give you a brief overview about
some important settings you should know.
One vital step when setting up the Köhler illumination is the sharp view of
the field diaphragm at the specimen level.
1) Focus the object to be viewed.
2) Close the field diaphragm so that only a small bright spot is visible in
the eyepiece.
3) Focus the image in the field diaphragm by using the condenser's height
adjustment.
Now the illuminated area is optimally set.
1) Open the field diaphragm so that its edge is still completely within the
visible area.
2) Center the image of the field diaphragm using the condenser's adjustment screws.
" A precisely adjusted condenser guarantees that only the area of the
specimen to be viewed is illuminated.
Objective, Total Magnification and Useful Magnification
Adjusting the Lamp
3) Repeat steps 1) and 2), until the edge of the field diaphragm is no longer visible in the visual field.
You can generally skip adjusting the lamp with modern microscopes, provided that the lamp is located in a frame and the bulb is in a centered socket.
Objective, Total Magnification and Useful Magnification
Terminology
Magnification
Numerical Aperture
Total magnification
Useful Magnification
33
Empty Magnification
There are no general rules for which objective you select; one should always
keep the specimen and reason for observation in mind.
The two most important parameters of an objective (magnification and numerical aperture) are engraved in the objective. First the magnification and
then the numerical aperture which is separated by a diagonal slash. Hence,
the code 40 / 0,65 marks an objective with a magnification of 40X and the
numerical aperture of 0,65.
An objective's engraved magnification is considerably important for the total
magnification. However, one should note that the range of useful magnification should not be surpassed.
The numerical aperture is the characteristic of an objective which determines its capacity. The numerical aperture can also be found engraved into
the objective. It is based on the refraction index of the lenses being used as
well as the objective's angular aperture. The manufacturers are narrowly
limited when dealing with the refraction index, whereas the objective's angular aperture can be increased by using appropriate measures. To achieve
this, a fluid with a high refraction index is placed between the cover slip and
the objective. The immersion objectives are accordingly marked and should
only be used with the appropriate immersion fluid.
The system's total magnification, to which an installed TV adaptor contributes, is based on the actual size of the final image. This can be the image
on the monitor, as well as the image printed on paper. To determine the total
magnification, it is best to use a stage micrometer which can be shown on
the monitor or the printout. You can determine the total magnification by dividing the length of the object shown by the set length on the micrometer.
The term useful magnification specifies a total magnification of the optical
system by which full resolution is used without having a negative effect on
contrast or sharpness. The useful magnification of the optical systems microscope depends on the numerical aperture being used ( engraved on the
objective). The useful magnification is in the range of 500x to 1,000x the numerical aperture.
A loss of contrast and sharpness is the result if total magnification is larger
than the useful magnification. Check to see if replacing the TV adapter with
a 0,5 or 0,63 model sufficiently reduces the total magnification. If this is not
possible, you can also use an objective with the same magnification, but
with a higher numerical aperture.
34
CC-12 • Objective, Total Magnification and Useful Magnification
Appendix II: Camera Installation
CC-12
Appendix II: Camera Installation
Warning
Please read the following carefully before attempting installation of
camera.
Requirements
Installation sequence
Warning
Windows 2000
What if my computer system already has a
FireWire interface?
The CC-12 can be used under the operating systems Windows 2000 and
Windows XP. The installation procedure is described separately for these
two operating systems.
In order to avoid problems, you’ll have to follow the steps of the procedure
in the order given here.
Install the image-analysis software first before connecting the camera
to the FireWire board.
1) Check whether your computer system has a FireWire interface onboard or a FireWire board installed.
2) Install the FireWire board provided with the camera.
3) Install the image-analysis software on your computer.
4) Install the camera and connect it to the computer system.
If your computer system already has a FireWire interface onboard or a separate FireWire board installed, you do not need to install the board provided
with the camera. If you are not sure whether a FireWire interface is available,
open the Windows Device Manager via Start > Settings > Control Panel >
System > Hardware > Device Manager....
35
An entry called ’IEEE 1394 Bus host controllers’ shows that the needed
FireWire interface is available.
CC-12 • Appendix II: Camera Installation
General Warnings!
• CCD image sensors are easily damaged by static discharge. Do not
disassemble the camera housing.
• Do not attempt to touch the internals of the camera with your bare
hands.
• Do not touch cover slip (over the CCD chip) with your hand or have any
•
•
•
•
•
•
object come in contact with glass surface. Should dust stick to the cover slip, blow it off gently with an air blower. (For dust stuck due to static
electricity, ionized air is recommended.)
It is best to use a LensPen to clean the cover slip. Do this only when
absolutely necessary.
Do not expose to strong light (sunlight) for long periods.
Exposure to high temperature or humidity will affect the characteristics
of the camera. Avoid storage or usage under such conditions.
Do not subject the camera to too much mechanical shock.
The FireWire cable plug is not symmetrical. You must make sure that
the cable plug is facing the same way as the camera socket when you
plug the cable into the camera jack.
Do not disconnect the FireWire cable while the image-analysis software is running.
The FireWire board IEEE-1394
Warning!
• The components of these devices are easily damaged by static discharge. Wear grounding wrist straps when handling them.
• Do not plug in or unplug the cards from the PCI slot while the PC is on.
• Please follow the installation manual in this booklet step by step to
36
avoid any incorrect connections. Incorrect connections may result in
serious damage to your PC system and/or the camera.
Installation Procedure - Windows 2000
Installation Procedure - Windows 2000
Installation of the FireWire board IEEE-1394
When is this necessary?
This procedure is necessary only if the PC does not have a FireWire interface on-board hardware configuration or if it is not yet equipped with a
FireWire board.
the FireWire board
How to install the
FireWire board
1) Turn off the PC and disconnect the power supply.
2) Remove the cover from the PC.
3) One PCI slot is required for the FireWire board. Insert the board into the
slot.
FireWire board installed
37
4) Replace the cover onto the PC.
5) You are now ready to first install the image-analysis software and then
the camera.
CC-12 • Installation Procedure - Windows 2000
Installation of the image-analysis software
Warning!
Requirements, PC
camera driver installation
Installation of the imageanalysis software
38
In the image-analysis software setup wizard select the
FireWire digitizer and select
your camera type. Select
the Firewire check box in the
Input Device (Digitizer)
Selection dialog box.
Then select the Soft Imaging System manufacturer
entry and the CC-12 camera
entry.
The image-analysis software must be installed first before connecting the
camera to the FireWire board.
The CC-12 camera requires the image-analysis software, (3.2 version), and
Microsoft Windows 2000 (or later). The image-analysis software also supports multithreading on multiprocessors, thus making full use of the power
of the latest multi-CPU PC’s.
Installation of the SIS View FireWire camera driver is simply done via the
image-analysis software setup. You are only required to select FireWire and
specify the additional camera driver during setup.
For installation, follow the instructions given on the monitor.
Installation of the image-analysis software
Precalibration
You will be advised to carry out a precalibration of the camera when installing the image-analysis software.
In this dialog box you can
activate the precalibration.
Note
Note
39
The camera’s serial number
is located below the
FireWire jack.
Select the TV adaptor's magnification from the list which has the same
name. Should you not be working with a remote microscope, clear the check
box. Then, mark the check box for all mounted objectives in the Magnification of the microscope objectives list.
Should a magnification appear on the microscope objective which does not
appear in the list, a manual calibration must be carried out for this objective.
A guide is part of the image analysis system's step by step guide.
The image-analysis software can support several same or different types of
FireWire cameras simultaneously. You differentiate CC-12 cameras via
their serial numbers when creating a new logical input channel via Image >
Set Input... > New Channel. A camera’s serial number is located directly
below the FireWire jack on the camera housing.
CC-12 • Installation Procedure - Windows 2000
Installation of the camera and cables
Don’t forget...
40
How to install the camera
Install the image-analysis software first before connecting the camera to the
FireWire interface.
1) The diagram shows a typical installation of a light microscope. You
should have all required components ready at hand.
2) Remove the protective cap from the camera.
3) Firmly screw the TV adaptor (0,5x or bigger") onto the camera. This
should be done with the CCD-chip side of the camera facing down to
minimize the chance of dust landing on its surface.
4) Install this assembly onto the microscope's camera mount. Some microscopes may require additional optical elements installed between
the camera mount and the C-mount adapter. You may also need to
align the orientation of the microscope with that of the stage, and also
match the focus of the camera image with that of the eyepiece image.
These settings can be done later when you are able to obtain an image
on the screen.
5) Plug one end of the FireWire cable into the camera connector.
" The FireWire cable plug is not symmetrical. You must make sure
that the cable plug is facing the same way as the camera socket
Installation of the camera and cables
when you plug the cable into the camera jack. The camera chip
may be ruined otherwise.
Make sure the cable plug is
facing the right way before
you plug it into the camera
socket
6) Plug the other end into one of the small connector sockets on the
FireWire board.
" There are two or more jacks depending on the FireWire board. You
can use any of them.
" The Digital Signature Not Found MS Windows dialog box(es)
appear on your screen. Confirm the message as often as necessary until the dialog boxes disappear.
41
7) Open the Windows Device Manager via Start > Settings > Control Panel > System > Hardware > Device Manager....
" The Device Manager dialog box contains the correct SIS FireWire
camera driver entry SoftHard Technology MV4.0 Camera. The
camera is ready for use.
CC-12 • Installation Procedure - Windows 2000
The Windows Device Manager shows the IEEE 1394 FireWire board and the SIS View FireWire camera driver SoftHard Technology MV4.0 Camera.
Troubleshooting
An error message appears when starting the program, even though you
have completely installed the camera and the image-analysis software:
42
What do I do if the camera cannot be found?
What to do when the
camera has been connected before the imageanalysis software installation
The image-analysis software has not been successful in creating a connection to the camera. Confirm the message with OK.
Check to see if the connector cable between the camera and the computer
has been correctly plugged-in. Should the connecter be loose or not plugged
in correctly, correct this by firmly plugging them into the corresponding jack.
Restart the image-analysis software. Should the problem still exist, continue
with the following section.
The error message No SIS-View FireWire camera has been found will also
appear when starting the program should the camera have been connected
before installing the image-analysis software. In this case, Windows 2000
has already installed its own camera driver, which prevents the error-free
communication between the camera and the computer. Installing the imageanalysis software now does not install the correct camera driver since this is
forbidden by Windows 2000. You will notice that the camera is not detected
by the image-analysis software when starting the application.
Troubleshooting
If the camera is hooked up to the computer, you have to install the correct
camera driver from the software’s setup CD separately from the software
installation. Do the following:
1) Select the Start > Settings > Control Panel > System > Hardware >
Hardware Wizard... command.
2) Click on the Next button.
43
3) Select the Add/Troubleshoot a device option and click on the Next button.
CC-12 • Installation Procedure - Windows 2000
44
4) Select the entry SoftHard Technology MV 4.0 from the Devices list and
click on the Next button.
5) Click on the Finish button.
Troubleshooting
6) Click on the Next button.
45
7) Select the Search for a suitable driver for my device (recommended)
option and click on the Next button.
CC-12 • Installation Procedure - Windows 2000
46
8) Insert the image-analysis software setup CD into your computer’s CDROM drive. Minimize the appearing setup dialog box.
9) Make sure that only the CD-ROM drives check box is selected and click
on the Next button.
" Wait until Windows has found the correct camera driver. The
driver's data file is mv40dcam.inf. You will find it on the CD-ROM in
Directory\program\support\cameras\sis_firewire\drivers\win2k.
10) Click on the Next button.
Troubleshooting
" The Digital Signature Not Found dialog box appears on your
screen.
11) Click Yes.
" Wait until the camera driver has been installed.
47
12) Click on the Finish button.
" The Digital Signature Not Found MS Windows dialog box(es)
appear on your screen. Confirm the message as often as necessary until the dialog boxes disappear.
CC-12 • Installation Procedure - Windows 2000
48
13) Open the Windows Device Manager via Start > Settings > Control Panel > System > Hardware > Device Manager.... The Device Manager dialog box contains the correct SIS FireWire camera driver entry
SoftHard Technology MV4.0 Camera.
The Windows Device Manager shows the IEEE 1394 FireWire board and the SIS View FireWire camera driver SoftHard Technology MV4.0 Camera.
14) Install the image-analysis software. During the installation check the
Firewire check box to in the Input Device (Digitizer) Selection dialog
box. Select the CC-12 (Firewire) check box in the following Camera Selection dialog box.
Installation Procedure - Windows XP
Installation Procedure - Windows XP
Installation of the FireWire board IEEE-1394
When is this necessary?
This procedure is necessary only if the PC does not have a FireWire interface on-board hardware configuration or if it is not yet equipped with a
FireWire board.
the FireWire board
How to install the
FireWire board
1) Turn off the PC and disconnect the power supply.
2) Remove the cover from the PC.
3) One PCI slot is required for the FireWire board. Insert the board into the
slot.
FireWire board installed
49
4) Replace the cover onto the PC.
5) You are now ready to first install the image-analysis software and then
the camera.
CC-12 • Installation Procedure - Windows XP
Installation of the image-analysis software
Warning
Requirements, PC
camera driver installation
Installation of the imageanalysis software
Install the image-analysis software first before connecting the camera to the
FireWire interface.
The CC-12 camera requires the image-analysis software, 3.2 version, and
Microsoft Windows (or later). The image-analysis software also supports
multithreading on multiprocessors, thus making full use of the power of the
latest multi-CPU PC’s.
Installation of the SIS View FireWire camera driver is simply done via the
image-analysis software setup. You are required to select FireWire and
specify the additional camera driver during setup. Then you have to get Windows to use the SIS View FireWire camera driver.
For installation, follow the instructions given on the monitor.
50
In the image-analysis software setup wizard select the
FireWire digitizer and select
your camera type. Select
the Firewire check box in the
Input Device (Digitizer)
Selection dialog box.
Related Topics
Installation of the camera driver, S. 53
Installation of the image-analysis software
Then select the Soft Imaging System manufacturer
entry and the CC-12 camera
entry.
Note
The image-analysis software can support several same or different types of
FireWire cameras simultaneously. You differentiate CC-12 cameras via
their serial numbers when creating a new logical input channel via Image >
Set Input... > New Channel. A camera’s serial number is located directly
below the FireWire jack on the camera housing.
The camera’s serial number
is located below the
FireWire jack.
51
CC-12 • Installation Procedure - Windows XP
52
How to install the camera
1) The diagram shows a typical installation of a light microscope. You
should have all required components ready at hand.
2) Remove the protective cap from the camera.
3) Firmly screw the TV adaptor (1/1,8" or bigger) onto the camera. This
should be done with the CCD-chip side of the camera facing down to
minimize the chance of dust landing on its surface.
4) Install this assembly onto the microscope's camera mount. Some microscopes may require additional optical elements installed between
the camera mount and the C-mount adapter. You may also need to
align the orientation of the microscope with that of the stage, and also
match the focus of the camera image with that of the eyepiece image.
These settings can be done later when you are able to obtain an image
on the screen.
5) Plug one end of the FireWire cable into the camera connector.
" The FireWire cable plug is not symmetrical. You must make sure
that the cable plug is facing the same way as the camera socket
when you plug the cable into the camera jack. The camera chip
may be ruined otherwise.
Installation of the camera driver
Make sure the cable plug is
facing the right way before
you plug it into the camera
socket
6) Plug the other end into one of the small connector sockets on the
FireWire board.
" There are two or more jacks depending on the FireWire board. You
can use any of them.
7) Continue with the camera driver installation before starting the imageanalysis software.
Installation of the camera driver
The SIS View FireWire camera driver is not signed and therefore not used
automatically under Windows XP. After having installed the image-analysis
software and connected the camera to the computer - or vice versa - you
have to go through the following steps to get Windows XP to use the SIS
View camera driver:
1) Open the Windows Device Manager via Start > My Computer > (right
mouse) > Properties > Hardware > Device Manager....
53
CC-12 • Installation Procedure - Windows XP
" The Device Manager dialog box shows the Generic 1394 Desktop
Camera entry in the Imaging devices section. The exclamation
mark indicates a device conflict.
2) Select the Generic 1394 Desktop Camera entry and click on the Update driver button in the device manager button bar.
54
3) Select the Install from list or specific location (advanced user) and click
on the Next button.
4) Select the Don’t search. I will choose the driver to install. option and
click on the Next button.
Installation of the camera driver
" The Model list contains the SoftHard Technology MV4.0 Camera
entry.
" If this entry is missing, click on the Cancel button. Repeat the instal-
lation of the image-analysis software including FireWire input
device and CC-12 camera selection.
5) Click on the Next button.
" The next Hardware Update Wizard dialog box will be opened in the
background displaying the message to wait while the Wizard
installs the software.
" Additionally, the Hardware Installation dialog box will pop up in the
foreground.
55
6) Click on the Continue Anyway button.
" The Hardware Update Wizard dialog box which had been in the
background, will become visible.
Related Topics
Installation of the image-analysis software, S. 50
CC-12 • Installation Procedure - Windows XP
" After having finished the SIS View FireWire camera driver installa-
56
tion the following dialog box will be opened.
7) Click on the Finish button to end the hardware update wizard.
" In the Device Manager dialog box there is still the same Generic
1394 Desktop Camera entry but the exclamation mark indicating
the device conflict should have disappeared. The camera is now
ready for use.
When encountering problems with the camera...
When encountering problems with the camera...
Should you have questions or should problems arise which you cannot solve
with the help of the documentation provided, please feel free to contact our
customer service - preferably via e-mail. Our customer-service personnel
will be more than happy to assist you.
57
1) Please clarify before hand when and under what circumstances the
problem arose.
• It would be best if you can clearly reproduce the problem/error. You
will therefore make it easier for you and our customer-service personnel to solve the problem.
2) Please write down the exact wording of the (error) messages which appear.
3) As an alternative, you can make "Snapshots" of these messages. You
can make a snapshot of your active window by using the key combinations [Alt+Print] respectively [Alt+Print]. A copy of the active window is
stored to your clipboard and can be easily attached to an e-mail using
[Strg+V] respectively [Ctrl+V].
4) Since very large e-mails can create transmission problems both for the
sender and the receiver, do not copy "screenshots" of the entire contents of the screen. For the same reasons it is advisable to reduce the
color depth of the snapshots.
5) Should the camera basically work, but problems with image quality
arise, save an image in which the problems are clearly visible in TIF format. Insert this example image in your e-mail to our customer service.
6) Start the image-analysis software.
7) Use the ? > Info... command to open the Info dialog box.
" The Info-Dialog box shows you information about the software
package (Docu, Auto, Pro), die Build-Number and the serial num-
CC-12 • When encountering problems with the camera...
ber of your analySIS-version as well as the operating system being
used.
• The software data mentioned should be at hand when contacting
our customer service personnel.
8) Please write an e-mail clearly describing the problem (including snapshots and example images), as well as the software data from the Info
dialog box to our customer service address:
[email protected]
• The quickest and most convenient way is to use the automatic e/
58
•
mail settings for your customer service query:
by using the ? > Info... > System Info... > Send button, an automatic
e-mail is created which you can then complete and send to us. If
you have made an example in TIF format, please attach this to the
e-mail as well. Before sending, read the notes in your e-mail box the
data about your system which you will send to us in this e-mail.
Should you not be able to send e-mails from your computer, use the
? > Info... > System Info... > Info save button to save the files and
to send them from another computer.
You may of course contact us via telephone or fax:
Tel.: (+ 49) 2 51 / 7 98 00-444
Fax: (+ 49) 2 51 / 7 98 00-99
CE DECLARATION OF CONFORMITY
Product Type
Brand Name
Model Designation
CCD Camera
Soft Imaging System
CC-12
Name and Address of manufacturer or representative
Manufacturer
Soft Imaging System GmbH
Johann-Krane-Weg 39
D-48147 Münster
Name and Address of manufacturer or representative
The product complies with the requirements of the following European directives:
89/336/EWG
Directive of the council on the approximation of the laws of Member
States relating to electromagnetic compatibility changed by directive
93/68/ECC of the council.
Compliance was proved by the application of the following
electromagnetic compatibility standards:
EN 55082-1 : 1997
EN 55022 : 1998 + A1 : 2000, cl. B
EN 50024 : 1998
EN 61000-4-2 : 1995 + A1 : 1998
EN 61000-4-3 : 1996 + A1 : 1998
ENV 50204 : 1995
EN 61000-4-4 : 1995
EN 61000-4-6 : 1996
Name and Address of manufacturer or representative
Date: April 30th, 2002
Dr Manfred Schierjott
April 30th, 2002
Date
Index
A
F
Acquisition, command 6
analySIS, version 2
Automatic Gain Display
Camera Control 10
Input 25
Firmware Version 24
Fluorescent microscopy 10
Black balance 11
intelligent Exposure 15
B
Binning 29
Black balance 11
C
Camera configuration 21
Camera Control 6
Black balance 11
Color Settings 7
Exposure time 7
Gain display 10
Sharpen filter 9
Sharpness Monitor 11
Camera Type 24
CC-12 Information 23
CC-12 Specifications 1
Clipping 29
Color Settings 7
Gamma Correction 8
Saturation 8
Configure Input 22
Display tab 25
Format tab 29
Input tab 22
Device ID 24
Device Information 23
Display tab 25
Image scaling 27
Over exposure 25
DLL Version, CC-12 Information 24
Duplicate Channels 22
E
Exposure time
Camera Control 7
Input 23
intX 14
Exposure time, intX 15
Gamma Correction
RGB mode 8
H
Housing temperature 24
I
Image scaling, Display tab 27
intelligent Exposure 6, 13
intX 13
Calibration 13
Exposure time 14
Exposure time correction 14
White balance 15
K
Köhler Illumination, Microscope 32
L
Live Acquisition 6
intX 14
Live overlay 6, 27
M
Microscope 31
Controlling illumination intensity 2
Empty Magnification 33
Illumination 31
Numerical Aperture 33
Objective 33
Total Magnification 33
Useful Magnification 33
Microscope Illumination Intensity 2
Microscope Settings 2, 31
Mirror 23
Monitor Settings 3
Monitor's Color Temperature 3
61
D
G
Index
N
U
NetCam 16
in the internet 18
In the intranet 17
Settings 19
New Channel 21
Useful magnification, Microscope 33
O
Online histogram 26
Over exposure 25
Over Exposure Warning 25
P
PC, Requirements 2
R
Requirements, PC 2
62
S
Select device 22
Serial Number 57
Serial number 24
Set Input 21
Sharpen filter 9
Sharpen filter Activate 23
Sharpness Monitor 11
Snapshot
Command 13
intX 14
Special 4
System Info 58
T
Tab Format 29
Binning 29
Clipping 29
Image format 29
Tab Input 22
Automatic gain display 25
Exposure time 23
Info 23
Mirror 23
Sharpen filter 23
Temperature 24
Total magnification, microscope 3
W
Web Server 17
White balance 3
Execute 4
General remarks 3
White balance, intX 15
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