Eppendorf Reference Bedienungsanleitung

®
Eppendorf Reference
Operating Manual
Eppendorf Reference®
1
Fig. / Abb. 1
Reference variabel / Reference adjustable
0.1 – 2.5 μL 10 – 100 μL 20 – 200 μL 100 – 1000 μL
0.5 – 10 μL
2 – 20 μL
Reference fix
1 – 50 μL
100 μL
1
500 – 2500 μL
200 – 250 μL
1
1
500 – 2500 μL
1
1
2
2
3
4
5
5
5
5
5
6
6
6
6
6
7
7
8
9
4
11
7
7
8
9
10
11
7
8
9
10
11
11
4
11
14
12
13
12
12
13
13
13
13
1
Teil A / Part A / Section A / Parte A / Parte A
Operating Manual ............................................................................4
Bedienungsanleitung ......................................................................22
Manuel d'Utilisation ........................................................................40
Manual de Instrucciones ................................................................58
Istruzioni per l'Uso ..........................................................................76
Part B / Teil B
Maintenance ....................................................................................94
Ordering Information ......................................................................102
Wartung ............................................................................................114
Bestellinformationen ......................................................................122
eppendorf and eppendorf Reference are registered trademarks.
Registered trademarks are not marked in all cases with TM or ® in this manual.
No part of this publication may be reproduced without the prior permission of the
copyright owner. Copyright© 2001 Eppendorf AG, Hamburg
2
3
Eppendorf Reference® – Part A – Table of contents
Operating Manual
1
2
2.1
2.2
3
4
5
5.1
5.2
6
7
7.1
7.2
7.3
7.4
7.5
8
8.1
8.2
8.2.1
8.2.2
8.2.3
9
9.1
9.2
9.3
10
10.1
4
Main Illustration ................................................................................5
Warning signs and hazard icons ....................................................6
Hazard icons ...................................................................................6
Danger levels ..................................................................................6
General safety notes ........................................................................7
Intended use .....................................................................................8
Technical data ..................................................................................9
Reference fix ...................................................................................9
Reference variable ..........................................................................9
Function principle ............................................................................10
Operation ..........................................................................................11
Volume setting .................................................................................11
Pipette tips .......................................................................................11
Aspirating liquid ...............................................................................11
Dispensing liquid .............................................................................12
Special notes ...................................................................................12
Testing / Alignment ..........................................................................13
Testing .............................................................................................13
Alignment ........................................................................................13
When should alignment be carried out? ..........................................13
Follow-up alignment in the case of error .........................................14
Adjustment for liquids with a density different to that of water ........16
Care / Sterilization ............................................................................17
Care .................................................................................................17
Sterilization ......................................................................................17
Decontamination prior to dispatch ...................................................18
Troubleshooting ...............................................................................19
If there is doubt that dispensing data are correct ............................21
Eppendorf Reference® – Part A – Chapter 1
1
Main Illustration
1
Control button
The control button is for setting the volume, dispensing and ejecting the pipette tip.
2
Volume display
Displays the preset volume.
3
Labeling area
The provided autoclavable, blank labels can be
labeled with a waterproof pen and affixed at this location.
4
Catch
Hold down the catch when you set the volume using
the control button.
5
Lower part
6
Ejector sleeve
7
Pipette tip
1
2
3
4
5
6
7
5
Eppendorf Reference® – Part A – Chapter 2
2
2.1
Warning signs and hazard icons
Hazard icons
Biological hazard
Health hazard
General hazard
Material damage
Notice
2.2
Danger levels
The danger level is part of a safety note and defines the potential
consequences if the information contained therein is not observed.
DANGER
Will lead to serious injuries or death.
WARNING
May lead to serious injuries or death.
CAUTION
May lead to minor or moderate injuries.
NOTICE
May lead to material damage.
Notice
Provides useful information.
6
Eppendorf Reference® – Part A – Chapter 3
3
WARNING
WARNING
CAUTION
CAUTION
General safety notes
WARNING! Damage to health due to handling infectious liquids and
pathogenic germs.
Observe the national regulations for handling these substances, the
biological security level of your laboratory, the material safety data sheets
and the manufacturer's application notes.
Wear your personal protective equipment (PPE).
Follow the instructions regarding hygiene, cleaning and decontamination.
For complete instructions regarding the handling of germs or biological
material of risk group II or higher, please refer to the "Laboratory
Biosafety Manual" (Source: World Health Organization, current edition of
the Laboratory Biosafety Manual).
WARNING! Damage to health due to toxic, radioactive or aggressive
chemicals.
Observe the national regulations for handling these substances as well
as the material safety data sheets and manufacturer's application notes.
Wear your personal protective equipment (PPE).
CAUTION! Danger to persons from improper handling.
Never aim the opening of a Reference equipped with pipette tip at
yourself or other persons.
Only initiate dispensing if it is safe to do so.
For all dispensing tasks, make sure that you are not endangering yourself
or anyone else.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by
Eppendorf may impair the safety, functioning and precision of the device.
Eppendorf cannot be held liable or accept any liability for damage resulting
from the use of incorrect or non-recommended accessories and spare parts
or from the improper use of such equipment.
Only use accessories and original spare parts recommended by
Eppendorf.
7
Eppendorf Reference® – Part A – Chapter 3 and 4
NOTICE
NOTICE
NOTICE
NOTICE
4
NOTICE! Damage to device from missing pipette tips.
Only use the Reference with attached pipette tips.
NOTICE! Carry-over, contamination and incorrect dispensing results
due to the repeated use of pipette tips.
The pipette tips are for single use only. Prolonged use can have a negative
impact on dispensing tasks.
Use the pipette tips only once.
Do not use autoclaved ep Dualfilter T.I.P.S. for dispensing.
NOTICE! Incorrect dispensing volume with special liquids and due to
temperature differences.
Solutions which differ greatly from water in terms of their physical data, or
temperature differences between the pipette, pipette tip and liquid can result
in incorrect dispensing volumes.
Avoid temperature differences between pipette, pipette tip and liquid.
Ensure that the temperature remains constant between 20 and 25 °C,
±0,5 °C.
Check the dispensing volume and readjust the pipette in case of
deviations.
NOTICE! Damage to device due to penetration of liquids.
Do not deposit the Reference with filled pipette tip.
Only use pipettes in the Reference-Serie when a pipette tip has been
attached.
Do not allow any liquids to penetrate the inside of the housing.
Intended use
The intended use is described in a separate document enclosed in the
package.
8
Eppendorf Reference® – Part A – Chapter 5
5
Technical data
5.1
Reference fix
Model /
Volume
μL
1
2
5
10
10
20
25
50
100
200
250
500
1,000
1,500
2,000
2,500
5.2
Color
code
epT.I.P.S.
light gray
light gray
light gray
light gray
yellow
yellow
yellow
yellow
yellow
blue
blue
blue
blue
red
red
red
0.5– 20
0.5– 20
0.5– 20
0.5– 20
2– 200
2– 200
2– 200
2– 200
2– 200
50–1,000
50–1,000
50–1,000
50–1,000
500–2,500
500–2,500
500–2,500
μL
Reference variable
Model
μL
Color
code
epT.I.P.S.
Volume
μL
μL
0.1– 2.5
dark
gray
0.1–
10
0.5–
light
gray
0.5–
20
0.5–
20
10
2–
20
light
gray
2–
20
yellow
2– 200
10– 100
yellow
2– 200
50– 200
yellow
2– 200
50– 250
blue
50–1,000
100–1,000
blue
50–1,000
500–2,500
red
500–2,500
0.25
1.25
2.5
1
5
10
2
10
20
2
10
20
10
50
100
50
100
200
50
100
250
100
500
1,000
500
1,250
2,500
Systematic
error
%
μL
± 2.5
± 2.0
± 1.5
± 1.0
± 1.0
± 0.8
± 0.8
± 0.7
± 0.6
± 0.6
± 0.6
± 0.6
± 0.6
± 0.6
± 0.6
± 0.6
± 0.025
± 0.04
± 0.075
± 0.1
± 0.1
± 0.16
± 0.2
± 0.35
± 0.6
± 1.2
± 1.5
±3
±6
±9
± 12
± 15
Systematic
error
μL
%
± 12.0
± 2.5
± 1.4
± 2.5
± 1.5
± 1.0
± 3.0
± 1.0
± 0.8
± 5.0
± 1.2
± 1.0
± 3.0
± 1.0
± 0.8
± 1.0
± 0.9
± 0.6
± 1.4
± 1.1
± 0.6
±3
±1
± 0.6
± 1.5
± 0.8
± 0.6
± 0.030
± 0.031
± 0.035
± 0.025
± 0.075
± 0.1
± 0.06
± 0.1
± 0.16
± 0.1
± 0.12
± 0.2
± 0.3
± 0.5
± 0.8
± 0.5
± 0.9
± 1.2
± 0.7
± 1.1
± 1.5
±3
±5
±6
± 7.5
±8
± 15
%
Random
error
± 1.8
± 1.2
± 0.8
± 0.5
± 0.5
± 0.3
± 0.3
± 0.3
± 0.2
± 0.2
± 0.2
± 0.2
± 0.2
± 0.2
± 0.2
± 0.2
%
μL
± 0.018
± 0.024
± 0.04
± 0.05
± 0.05
± 0.06
± 0.075
± 0.15
± 0.2
± 0.4
± 0.5
±1
±2
±3
±4
±5
Random
error
μL
± 6.0
± 1.5
± 0.7
± 1.8
± 0.8
± 0.4
± 2.0
± 0.5
± 0.3
± 1.5
± 0.6
± 0.3
± 0.7
± 0.3
± 0.15
± 0.3
± 0.3
± 0.2
± 0.3
± 0.3
± 0.2
± 0.3
± 0.2
± 0.2
± 0.3
± 0.2
± 0.2
± 0.015
± 0.019
± 0.018
± 0.018
± 0.04
± 0.04
± 0.04
± 0.05
± 0.06
± 0.03
± 0.06
± 0.06
± 0.07
± 0.15
± 0.15
± 0.15
± 0.3
± 0.4
± 0.15
± 0.3
± 0.5
± 0.3
±1
±2
± 1.5
±2
±5
9
Eppendorf Reference® – Part A – Chapter 5 and 6
The technical data is only valid when the quoted eppendorf pipette tips are used.
Tests carried out in accordance with ISO 8655 for piston-stroke pipettes with an
air cushion using a precision balance with evaporation trap approved by the
standardization authorities.
Number of determinations:
10; degassed, bidistilled water, 20 °C – 25 °C, constant to ± 0.5 °C; with prewetted pipette tip; dispensing carried out on inner wall of vessel. If the place
where the pipette is used is at extremely high altitude, an adjustment must be
made in line with the ambient air pressure.
6
Function principle
The pipettes in the Reference-Serie are piston-stroke pipettes that operate according to the air-cushion principle.
The Reference-Serie consists of fixed-volume pipettes and pipettes with an
adjustable volume setting.
The control button is multi-functional. The function executed by the pipette
depends on how far its control button is pressed down.
Ex
20 °C
Three steps are necessary to operate the pipette:
1. Measuring stroke
Press the control button down until the first stop. The desired volume of liquid
is dispensed or, when the button is released, aspirated.
2. Blow-out
Press the button down a little more until the next stop.
Any liquid remaining in the pipette tip is emptied.
3. Ejection
Press the button all the way down.
The pipette tip is ejected.
10
Eppendorf Reference® – Part A – Chapter 7
7
Operation
The pipette can be individually labelled. The autoclavable blank adhesive label
provided can be marked with a permanent marker and fits onto the identification
area on the top of the housing.
7.1
Volume setting
The volume is adjusted by pressing down the lateral catch and turning the control
button at the same time.
It is advisable to carry out volume setting from the higher down to the lower value.
i.e. first go above the desired volume and then return to the lower value.
7.2
Pipette tips
The pipette can function only when a pipette tip is attached into which the
liquid is aspirated.
To facilitate the search for a suitable tip, the color of the control buttons
corresponds to the color of the eppendorf tip racks.
When pipetting liquids with wetting properties different to those of water, please
observe the recommendations contained in Chapter 7.5.
7.3
Aspirating liquid
– The liquid which is to be aspirated is taken from a suitable vessel.
– Attach suitable pipette tip to the pipette firmly (observe the color coding).
– Press down the control button to the first stop (measuring stroke).
– Immerse the pipette tip vertically approx. 3 mm into the liquid.
– Allow the control button to slide back slowly.
– Pull the tip out of the liquid slowly.
– To remove any remaining droplets, dab with non-fibrous cellulose material.
When doing so, ensure that no liquid comes out of the tip.
11
Eppendorf Reference® – Part A – Chapter 7
7.4
Dispensing liquid
– Hold the tip at an angle against the inside wall of the tube.
– Press down the control button slowly to the first stop (measuring stroke) and
wait until the liquid stops flowing.
– Press down the control button to the second stop (blow-out) until the tip is completely empty.
– Hold down the control button and pull the tip up the inner wall of the tube.
– Tip is ejected by pressing the control button to the final stop.
7.5
Special notes
To guarantee the highest degree of precision and accuracy, we recommend prewetting all new tips by aspirating and dispensing liquid two or three times before
pipetting.
Finally, with the tip not in contact with the liquid, empty it completely on the inner
wall of the tube (via blow-out).
Explanation: Why does the pipette tip have to be pre-wetted?
To compensate for the properties of the liquid.
Wetting liquids (serum, detergent) form a thin film on the inner wall of the pipette
tip. When the first pipetting is carried out, the volume dispensed would thus be too
low.
When pipetting serum or high-viscosity solutions, wait a few seconds when
aspirating and dispensing liquid.
12
Eppendorf Reference® – Part A – Chapter 8
8
Testing / Alignment
The serial number of the pipette is located on its control button.
8.1
Testing
Distilled water is dispensed from a pre-wetted tip into a tube and is then weighed.
Volumes 0.1 – 10 μL:
The test is performed with a highly sensitive analytic balance (resolution balance:
0.001 mg) by releasing the volume.
The bidistilled water, weighing vessel, pipette and pipette tip must all
be the same temperature!
To calculate the volume, divide the weight by the density of the water
(at 20 °C: 0.9982 mg/μL; mg/μL = g/mL).
Volumes > 10 – 2.500 μL:
For a volume of more than 10 μL, a balance with a resolution of 0.01 mg is
sufficient.
8.2
Alignment
8.2.1 When should alignment be carried out?
The pipettes in the Reference-Serie were tested during production in accordance
with the measurement conditions for water listed in Chapter 2.
In the case of doubts arising about the accuracy of the pipetted volume, the
following points should first be checked:
– Is the pipette leaking? (This is one possible reason for dispensed volumes
being too low; troubleshooting and solutions are contained in Chapter 7)
– What is the temperature of the sample? (In open tubes, water at room
temperature cools down due to evaporation.)
– What is the temperature of the pipette?
– What is the temperature of the air?
– Has mg been converted into μL?
– Does the sample have a different density to that of water?
– Is the pipetting speed too high?
13
Eppendorf Reference® – Part A – Chapter 8
If the place where the pipette is used is at extremely high altitude, an adjustment
must be made in line with the ambient air pressure. At 1,000 m above sea level,
there is a volume error of approx. –0.4 %.
Assistance with these questions is contained in eppendorf‘s SOP (Standard
Operating Procedure). An SOP can be called up from our home page
www.eppendorf.com
If these checks prove to be unsuccessful, it is safe to assume that the
alignment of the pipette has altered (e.g. due to several components having
been replaced).
8.2.2 Follow-up alignment in the case of error
From a technical point of view, this is a zero-point shift. The value by which the
setting of the pipette is shifted remains constant across the entire measuring
range. If, for example, in the case of a 10 – 100 μL pipette, follow-up alignment
of 1 μL is carried out at 100 μL (=1 %), the pipette is also adjusted by 1 μL at
10 μL (= 10 %!)
Alignment Reference fix:
To assist you in finding the basic setting again, round adhesive labels with an
arrow are provided as an alignment aid.
– Stick the alignment aid onto the control button.
Determine the volume by weighing and calculation
( see point a – c "Alignment Reference variable").
d
CAL
14
Pierce the calibration seal attached to the opening
for adjustments with Side B of the key provided.
This destroys the seal which should be removed.
Undo the screw inside a little until the control button
can be turned.
Eppendorf Reference® – Part A – Chapter 8
e
Adjust control button by the volume determined.
One revolution of the control button corresponds to the following values for
water:
Reference fix
Vol./revol.
1, 2, 5, 10 μL
10, 20 μL
25, 50 μL
100 μL
200, 250 μL
500, 1000 μL
1500, 2000, 2500 μL
approx.
0.5
approx.
1
approx.
2.4
approx.
5
approx. 12
approx. 46
approx. 118
Clockwise rotation:
Counterclockwise rotation:
f
μL
μL
μL
μL
μL
μL
μL
decrease in volume.
increase in volume.
Tighten the screw until the control button can no longer be turned.
Then continue as described in steps f and g of the Alignment Reference variable.
If the nominal volume does not correspond with the measuring result, repeat steps
d – g.
Then reseal the adjustment opening using one of the calibration seals supplied.
Alignment Reference variable:
a
The pipette, tip and water must all be the same temperature
(20 – 25 °C, constant to ± 0.5 °C).
b
Set the Reference variable to the desired nominal volume.
c
With a pipette tip attached to the pipette, the desired volume is pipetted and
weighed 10 times. The average of this weighing is converted into μL using
the following formula:
Weight
Volume =
Density of liquid
(at the temperature specified)
The value obtained is the actual setting
(density of water at 20 °C: 0.9982 mg/μL; mg/μL = g/mL).
15
Eppendorf Reference® – Part A – Chapter 8
d
CAL
e
Pierce the calibration seal attached to the opening
for adjustments with Side B of the key provided.
This destroys the seal which should be removed.
Carefully attach the key to the adjusting sleeve
inside.
Turn the wrench to adjust the volume display of the pipette (with piston stroke
unchanged) to the actual volume (measurement under step c).
f
Remove the wrench.
g
Repeat step c). The readings must be within the tolerances specified in the
technical data.
If the nominal value still does not agree with the measuring result, repeat
steps d – g.
Since this adjustment affects the entire measuring range, it is imperative to check
the other volumes of this pipette specified in the technical data.
Then reseal the adjustment opening using one of the calibration seals supplied.
8.2.3 Adjustment for liquids with a density different to that of water
It is possible to adjust the pipette for one specific volume of liquid with a density
different to that of water in such a way that the volume displayed corresponds to
the volume pipetted.
All other values for the adjustable pipettes are now out of alignment, i.e. an
adjustable-volume pipette has been converted into a fixed-volume pipette!
Proceed as described in Chapter 8.2.2.
A pipette set in this way delivers a pipetting value that correlates with that in
the display only for the liquid used and for the volume tested! For this
reason, we very strongly recommend labeling the converted pipette as a
fixed-volume pipette for "Solution y”!
The error for liquids with a higher vapor pressure (e.g. organic solvents)
cannot be aligned in this way. In this case, we recommend using an
eppendorf positive-displacement pipette.
16
Eppendorf Reference® – Part A – Chapter 9
9
9.1
Care / Sterilization
Care
Depending on the frequency of use, all parts of the pipette should be cleaned from
time to time in a soap solution or should be carefully wiped clean using
isopropanol. They should then be rinsed in distilled water and dried.
The seals are maintenance-free and the pistons should be lubricated lightly (using
the grease for pipettes provided) when cleaned or replaced.
Severe contamination caused by the liquid entering the pipette can be removed
after the pipette has been disassembled (see Part B, Maintenance).
For information about replacing defective parts, please see Part B, Maintenance.
9.2
Sterilization
The Reference-Serie including the blank label provided (marked with a permanent
marker) is fully autoclavable at 121 °C for 20 minutes.
Before autoclaving, unscrew the pipette at the central junction by rotating about
one revolution. This enables steam to penetrate more easily into the pipette during
autoclaving.
After autoclaving, the pipette may have to be dried at room temperature. Retighten
the central junction only after the pipette has completely cooled.
The tip cone may have to be tightened again with the wrench (see part B, Maintenance).
The Reference pipettes may be temporarily subjected to the UV light of a sterile
bench (≥ 254 nm).
17
Eppendorf Reference® – Part A – Chapter 9
9.3
Decontamination prior to dispatch
If the Reference pipette is to be checked, repaired or calibrated by
Eppendorf AG or another service partner, it must be free of
hazardous substances and clean!
A form called "Decontamination certificate for return of goods" and general notes
about decontamination are available on our home page:
www.eppendorf.com
A signed decontamination certificate must be enclosed with the pipette when it is
returned. The serial number of the Reference must be entered on the
decontamination certificate.
The Reference can be decontaminated of potentially infectious substances by
being steam-autoclaved (see Sec. 9.2).
The surfaces of the pipette can be disinfected with alcohol (ethanol, propanol) or
with a disinfectant containing alcohol.
18
Eppendorf Reference® – Part A – Chapter 10
10
Troubleshooting
Error
Cause
Solution
Droplets on
the inner wall
of the pipette
tip.
– Uneven wetting of the
plastic wall.
– A pipette tip with poor
wetting properties has
been used.
– Attach a new pipette tip.
Pipette is
dripping
and/or the
volume
pipetted is
incorrect.
– The tip is loose.
– Press the tip firmly in place.
– A poorly fitting pipette tip
– Use an original eppendorf
has been used.
tip.
– Liquid with a high vapor
– In this case, we recommend
pressure has been pipetted.
pipetting using a positivedisplacement pipette.
– Tip has been taken out of
– Remove the tip slowly from
the liquid too quickly.
the liquid.
– Use an original eppendorf
tip.
The pipette is dripping
because:
– Piston is contaminated.
– Piston is damaged.
– Seals are damaged.
– Tip cone loose.
– Clean and lightly lubricate
the piston.
– Replace the piston and seal
(see Part B, Maintenance).
– Replace all seals
(see Part B, Maintenance).
– Lightly tighten tip cone with
wrench (see Part B,
Maintenance).
Exchange, where
necessary.
19
Eppendorf Reference® – Part A – Chapter 10
Error
Cause
Solution
Control button
jams, moves
erratically.
– Piston contaminated.
– Clean piston and lubricate
lightly.
– Disassemble pipette. Clean
all seals and exchange
where necessary
(see Part B, Maintenance).
– Unscrew pipette at the
central junction and
ventilate.
Clean piston and lubricate
lightly.
– Seals contaminated.
– Penetration of solvent
vapors.
Pipette
– Liquid has penetrated the tip – Unscrew pipette at the
blocked,
cone and dried.
central junction, rinse lower
too little liquid is
part first with warm water,
aspirated.
then with distilled water and
allow to dry.
Or:
– Disassemble pipette.
Replace ejector seal in the
tip cone (see Part B, Maintenance).
For 25 to 500 μL pipettes:
For 25 to 50 μL:
– The filling tube in the nose
cone is blocked.
– Replace tip cone.
For 100 to 500 μL:
– Replace the filling tube in
the tip cone (see Part B,
Maintenance).
20
Eppendorf Reference® – Part A – Chapter 10
10.1
If there is doubt that dispensing data are correct
To avoid dispensing errors, the precision and accuracy of the Reference pipettes
need to be checked regularly. The PICASO II software program (see ordering
information) is available to determine permitted systematic and random
measuring deviation (see Chapter 2 "Technical data"). An SOP (Standard
Operating Procedure) for checking pipettes can be called up from our home page
www.eppendorf.com.
For liquids whose physical data deviate significantly from those of water,
calibration needs to be changed in accordance with Chapter 8.2.3.
Calibration will also need to be changed if the pipette is being used at a very high
altitude.
21
Eppendorf Reference® – Part B – Contents
Maintenance
Maintenance
I
II
III
IV
Exchanging the piston ..................................................................95
Exchanging the seals ....................................................................95
Removing the seals .........................................................................96
Inserting the seals ..........................................................................101
Exchanging the filling tube ..........................................................101
Exchanging the tip cone ...............................................................101
Ordering information
I
II
III
IV
Reference fixed-volume ................................................................102
Reference adjustable-volume ......................................................106
Additional accessories .................................................................109
Pipette tips .....................................................................................110
Please only use the accessories recommended by eppendorf.
Using spare parts and disposables which we have not recommended
can reduce the precision, accuracy and life of the pipette. We do not
honor any warranty or accept any responsibility for damage resulting
from such action.
Guarantee and warranty
In the case of warranty claims please contact your responsible Eppendorf contractual partner.
Wear-and-tear parts are excluded from the warranty. When returning the pipette to
the Eppendorf contractual partner the necessary data for utilization and the performance of decontamination (see Part A section 6.3) should be included.
Servicing must be carried out by the Service of the Eppendorf contractual partner.
No warranty shall apply with misuse or opening of the device by unauthorized persons.
For information on replacing pistons and seals as well as on disassembling and
assembling the different models in the Reference-Serie please see page 1 at the
front of this manual.
The fix and variable pipettes are of identical construction. You should therefore
refer to the figure corresponding to the volume of your pipette or the volume range
it falls in.
94
Eppendorf Reference® – Part B – Maintenance
I
Exchanging the piston
– Unscrew pipette at the central junction.
– Press control button and hold down. Hold piston at the top of the piston mounting and pull off. If the piston fits too tightly, the spring at the piston mounting
can be pressed down slightly with side B of the wrench and the piston then pulled off.
– Attach new piston down to the stop and lubricate lightly.
II
Exchanging the seals
The lower parts of the Reference-Serie can be completely disassembled for
cleaning and maintenance using the wrench provided.
The wrench has the following functions:
%
'
$
A = Narrow opening: for loosening and tightening the tip cone.
Wide opening (D): For tightening the tip cone (with the lettering
facing the pipette tip).
The wrench is designed in such a way that the tip cone cannot be
tightened too much.
B =For loosening the screw in the tip cone.
During assembly, for mounting the screw, spring and seal and tightening the screw.
During alignment, for adjusting the volume display.
C =For removing the seal in the tip cone.
&
95
Eppendorf Reference® – Part B – Maintenance
Removing the seals
Fig. 2 – 6 on the following pages show you how to remove the seals. The numbers
shown are identical with the numbers in the Ordering information (page 94) and
the numbers of the parts on page 1 of this manual.
0.1 – 2.5 μL, 0.5 – 10 μL and 2 – 20 μL (Fig. 2)
Push the control button all the way down and pull
off the ejector sleeve (13).
Piston seal
The piston seal in the tip cone is exchanged by
replacing the entire tip cone (11).
Tighten the tip cone (see IV of this part).
B
A
C
11
13
96
Eppendorf Reference® – Part B – Maintenance
10 – 100 μL ( Fig. 3)
Push the control button all the way down and pull
off the ejector sleeve (13).
Piston seal (4)
– Unscrew pipette.
– Discard seal (4) with O-ring.
– Insert new seal (see "Inserting the seals").
Tighten the tip cone (see IV of this part).
The tip cone (11) is also available complete with
seal.
B
B
A
C
A
A
C
C
4
B
11
13
12
97
Eppendorf Reference® – Part B – Maintenance
50 – 200 μL ( Fig. 4)
Push the control button all the way
down and pull off the ejector
sleeve (13).
Piston seal (4)
– Unscrew pipette.
– Remove piston seal (4) from tip
cone (with the piston in the grip of
the pipette) and discard.
– Place new piston seal onto tip cone.
Tighten the tip cone (see IV of this
part).
B
A
C
4
4
14
13
12
11
98
Eppendorf Reference® – Part B – Maintenance
50 – 250 μL ( Fig. 5)
Push the control button all the way down and pull off the
ejector sleeve (13).
Piston seal (4)
– Unscrew pipette at the central junction.
– Discard piston seal (4). Insert new piston seal into the
clamp (3) and place both into the lower part (5).
Ejector seal (10)
– Unscrew lower part of the pipette.
– Discard seal (10) with O-ring.
– Insert new seal (see "Inserting the seals").
Tighten the tip cone (see IV of this part).
The tip cone (11) is also available complete with seal.
3
4
B
5
C
A
B
A
A
C
C
10
B
11
12
13
99
Eppendorf Reference® – Part B – Maintenance
100 – 1000 and 500 – 2500 μL (Fig. 6)
Push the control button all the way down and pull off the
ejector sleeve (13).
Piston seal (4)
– Unscrew pipette at the central junction.
– Discard piston seal (4) and replace.
Ejector seal (10)
– Unscrew lower part of the pipette.
– Discard seal (10) with O-ring.
– Insert new seal (see "Inserting the seals").
Tighten the tip cone (see IV of this part).
The tip cone (11) is also available complete with ejector
seal.
B
C
A
A
4
C
B
B
A
C
11
13
100
10
Eppendorf Reference® – Part B – Maintenance
Inserting the seals
$
&
%
'
Pull new seal off the pin and push screw, spring, seal (with plastic part first) in that
order onto side B of the wrench as shown in the figure and screw lightly into tip
cone. Do not tighten too much. Assemble pipette again.
III
Exchanging the filling tube
10 – 100 μL, 50 – 200 μL and 50 – 250 μL (Fig. 3, 4, 5)
Filling tube (12)
– Unscrew lower part of the pipette.
– Push filling tube (12) (and damping tube (14) for 50 – 250 μL) out of the tip
cone from below with the wire punch. Push in new tube from above.
IV
Exchanging the tip cone
After the ejector sleeve has been pulled off, the tip cone together with the
seal (11) can be loosened with the wrench (A, narrow opening) and exchanged
(together with the seal. See Fig. 2 – 6).
Tighten the tip cone:
Place wrench with the wide opening (A, lettering facing the pipette tip) onto the tip
cone and tighten until it locks into position by turning half a revolution.
The wrench is designed in such a way that the tip cone cannot be tightened too
much.
Caution
After exchanging parts or completing other maintenance, always check that the
pipette functions correctly (see Part A, Section 5 and 7 of this Manual).
If a problem cannot be solved with the aid of the recommendations above, please
return the eppendorf Reference to your eppendorf appointed dealer.
101
Eppendorf Reference® – Part B – Ordering information
Ordering Information
I
Reference fixed-volume
(Please see page 1 (Fig. 1) of this manual).
Only parts with order numbers are available separately.
Pipettes / spare parts
Models
Gray control button (for 10 μL pipette tips)
1 μL
2 μL
5 μL
10 μL
Yellow control button (for 200 μL pipette tips)
10 μL
20 μL
25 μL
30 μL
50 μL
75 μL
80 μL
90 μL
100 μL
150 μL
200 μL
Blue control button (for 1000 μL pipette tips)
200 μL
250 μL
300 μL
500 μL
800 μL
900 μL
1000 μL
Red control button (for 2500 μL pipette tips)
1500 μL
2000 μL
2500 μL
102
Order number Order number
International
North America
4900 000.010
4900 000.028
4900 000.036
4900 000.044
022470400
022470451
022470507
022470558
4900 000.109
4900 000.117
4900 000.150
022470604
022470752
022470809
022470850
022470957
022471007
022471058
022471104
022471155
022471201
022471252
4900 000.125
4900 000.133
4900 000.508
4900 000.540
4900 000.524
022471309
022471350
022471457
022471554
022471601
022471651
4900 000.923
4900 000.907
4900 000.915
022471708
022471759
022471805
4900 000.516
Eppendorf Reference® – Part B – Ordering information
Order number Order number
International
North America
1 Piston
1 – 10 μL (gray control button)
20 μL
10 – 20 μL (yellow control button)
25 – 50 μL
100 μL, incl. piston seal (4)
75 – 100 μL, incl. piston seal (4)
150 – 200 μL
250 μL
200 – 250 μL, incl. piston seal (4)
300 – 1000 μL, incl. piston seal (4)
500 – 1000 μL, incl. piston seal (4)
500 – 2500 μL, incl. piston seal (4)
2 Piston spring
1 – 250 μL
250 μL
300 – 1000 μL
500 – 1000 μL
1500 – 2500 μL
3 Clamp
4910 810.001
4910 811.008
4900 810.003
4910 812.004
4910 813.000
4910 814.007
4910 815.003
4910 825.009
4910 826.005
4910 827.001
022475088
022475100
022475118
022475126
022475142
022475169
022475185
022475207
022475223
022475240
022475266
not sold separately
4 Piston seal
75 – 100 μL, incl. screw (8), spring (9)
100 μL, incl. screw (8), spring (9) 4910 820.007
150 – 200 μL, incl. screw (8), spring (9)
200 – 250 μL, incl. screw (8), spring (9), 4910 821.003
ejector seal (10)
250 μL, incl. screw (8), spring (9),
ejector seal (10)
300 – 1000 μL, incl. screw (8), spring (9),
ejector seal (10)
500 – 1000 μL, incl. screw (8), spring (9), 4910 822.000
ejector seal (10)
500 – 2500 μL, incl. screw (8), spring (9), 4910 823.006
ejector seal (10)
022475282
022475304
022475321
022475347
022475363
103
Eppendorf Reference® – Part B – Ordering information
5 Lower housing
6 Ejector, includes ejector spring (7)
1 – 20 μL, incl. ejector tube
25 – 100 μL, incl. ejector tube
150 – 200 μL
200 – 250 μL
250 μL
300 – 1000 μL
500 – 1000 μL
1500 – 2500 μL
Order number Order number
International
North America
not sold separately
4910 839.000
4910 840.008
4910 841.004
4910 842.000
4910 843.007
022475380
022475401
022475428
022475461
022475487
022475509
7 Ejector spring
(1 – 100 μL includes ejector tube)
not sold separately
8 Screw for tip cone
not sold separately
9 Tip cone spring
not sold separately
10 Ejector seal
not sold separately
11 Tip cone, complete
1 – 10 μL gray, incl. seal
10 – 20 μL yellow, incl. seal
25 – 50 μL, incl. (8), (9), (4), (12)
75 – 100 μL, incl. (8), (9), (4), (12)
100 μL, incl. (8), (9), (4), (12)
150 – 200 μL, incl. (12), (14)
200 – 250 μL, incl. (8), (9), (10), (12)
250 μL, incl. (8), (9), (10), (12)
300 – 500 μL, incl. (8), (9), (10), (12)
500 μL, incl. (8), (9), (10), (12)
800 – 1000 μL, incl. (8), (9), (10)
1000 μL, incl. (8), (9), (10)
500 – 2500 μL, incl. (8), (9), (10)
12 Filling tube (5 pieces, 1 wire punch)
75 – 100 μL
100 μL
150 – 200 μL
200 – 250 μL
250 μL
300 – 500 μL
500 μL
104
4910 830.002
4910 832.005
4900 830.004
4910 833.001
4910 834.008
4900 831.000
4910 835.004
4910 836.000
4910 837.007
4910 838.003
4900 837.009
022475541
022475584
022475606
022475622
022475649
022475665
022475681
022475703
022475720
022475746
022475762
022475789
022475801
Eppendorf Reference® – Part B – Ordering information
Order number Order number
International North America
13 Ejector sleeve
1 – 100 μL
150 – 200 μL
200 – 1000 μL
250 – 1000 μL
500 – 2500 μL
4910 845.000 022475827
022475843
4910 846.006
022475860
4910 847.002 022475886
14 Damping tube
not sold separately
Lower part complete, incl. piston and (3) – (13)
1 – 10 μL
10 μL (gray button)
10 – 20 μL (yellow button)
25 – 50 μL
75 – 100 μL
100 μL
150 – 200 μL
200 – 250 μL
250 μL
300 – 500 μL
500 μL
800 – 1000 μL
1000 μL
500 – 2500 μL
4910 890.005
022475924
4910 892.008 022475967
4900 890.007 022475983
022476009
4910 893.004
022476025
4910 894.000
022476041
022476068
4900 891.003
022476084
4910 895.007
4910 896.003 022476106
Reference repair set
4910 805.008 022475045
(Grease for pipettes (1 g), 1 wrench, 6 blank
labels, 1 wire punch, 1 filling tube each for 75 –
100 μL, 150 – 200 μL, 250 μL and 300 – 500 μL, 1
damping tube for 50 – 200 μL)
Calibration aid labels (5 pieces)
4900 805.000 022475002
Grease for pipettes (1 g)
0013 063.010 022458507
Wrench
4910 092.001 022475029
Calibration seal, red (5 pieces)
4910 601.003
105
Eppendorf Reference® – Part B – Ordering information
II
Reference adjustable-volume
(Please see page 1 (Fig. 1) of this manual).
Only parts with order numbers are available separately.
Pipettes / spare parts
Models
Dark gray control button (for 10 μL pipette tips)
0.1 – 2.5 μL
Order number Order number
International North America
4910 000.085 022470001
Light gray control button (for 20 μL pipette tips)
0.5 – 10 μL
2 – 20 μL
4910 000.018 022470051
4910 000.026 022470108
Yellow control button (for 200 μL pipette tips)
2 – 20 μL
10 – 100 μL
50 – 200 μL
4910 000.034 022470159
4910 000.042 022470205
4910 000.093 022470256
Blue control button (for 1000 μL pipette tips)
50 – 250 μL
100 – 1000 μL
4910 000.050
4910 000.069 022470302
Red control button (for 2500 μL pipette tips)
500 – 2500 μL
4910 000.077 022470353
1 Piston
0.1 –
0.5 –
2 –
10 –
50 –
50 –
100 –
500 –
4910 809.003
4910 810.001
4910 811.008
4910 812.004
4910 816.000
4910 813.000
4910 814.007
4910 815.003
2.5 μL
10 μL
20 μL
100 μL, incl. piston seal (4)
200 μL, incl. piston seal (4)
250 μL, incl. piston seal (4)
1000 μL, incl. piston seal (4)
2500 μL, incl. piston seal (4)
022475061
022475088
022475100
022475126
022475142
022475185
022475207
2 Piston spring
50 – 250 μL
100 – 1000 μL
500 – 2500 μL
4910 825.009
4910 826.005 022475240
4910 827.001 022475266
3 Clamp
not sold separately
106
Eppendorf Reference® – Part B – Ordering information
Order number Order number
International North America
4 Piston seal
10 – 100 μL, incl. screw (8), spring (9)
4910 820.007
50 – 200 μL, with 2 O-rings
4910 824.002
50 – 250 μL, incl. screw (8), spring (9), ejec-4910 821.003
tor seal (10)
100 – 1000 μL, incl. screw (8), spring (9),
4910 822.000
ejector seal (10)
500 – 2500 μL, incl. screw (8), spring (9),
4910 823.006
ejector seal (10)
022475282
022475304
022475347
022475363
5 Lower housing
not sold separately
6 Ejector, includes ejector spring (7)
0.1 – 2.5 μL, incl. ejector tube
0.5 – 10 μL, incl. ejector tube
2 – 20 μL
10 – 100 μL
50 – 200 μL
50 – 250 μL
100 – 1000 μL
500 – 2500 μL
4910 839.000
4910 839.000
4910 839.000
4910 840.008
4910 844.003
4910 841.004
4910 842.000
4910 843.007
022475380
022475380
022475380
022475401
022475444
022475487
022475509
7 Ejector spring
(0.1–2.5, 0.5–10, 2–20, 10–100 μL incl. ejector tube)not sold separately
8 Screw for tip cone
not sold separately
9 Tip cone spring
not sold separately
10 Ejector seal
not sold separately
11 Tip cone, complete
0.1 – 2.5 μL, incl. seal
0.5 – 10 μL, incl. seal
2 – 20 μL, light gray, incl. seal
2 – 20 μL, yellow, incl. seal
10 – 100 μL, incl. (8), (9), (4), (12)
50 – 200 μL, incl. (12), (14)
50 – 250 μL, incl. (8), (9), (10), (12)
100 – 1000 μL, incl. (8), (9), (10)
500 – 2500 μL, incl. (8), (9), (10)
4910 829.004
4910 830.002
4910 831.009
4910 832.005
4910 833.001
4910 851.000
4910 834.008
4910 835.004
4910 836.000
022475525
022475541
022475568
022475584
022475622
022475649
022475703
022475720
107
Eppendorf Reference® – Part B – Ordering information
Order number Order number
International North America
12 Filling tube, (5 pieces, 1 wire punch)
10 – 100 μL
4910 837.007 022475746
50 – 200 μL, incl. 2 pieces of (14)
4910 853.002 022475762
50 – 250 μL
4910 838.003
13 Ejector sleeve
0.1 – 2.5 μL
4910 845.000 022475827
0.5 – 10 μL
4910 845.000 022475827
2 – 20 μL
4910 845.000 022475827
10 – 100 μL
4910 845.000 022475827
50 – 200 μL
4910 852.006 022475843
50 – 250 μL
4910 846.006
100 – 1000 μL
4910 846.006 022475860
500 – 2500 μL
4910 847.002 022475886
14 Damping tube
not sold separately
Lower part, complete, incl. piston and (3) – (14)
0.1 – 2.5 μL
4910 889.007 022475908
0.5 – 10 μL
4910 890.005 022475924
2 – 20 μL, light gray
4910 891.001 022475941
2 – 20 μL, yellow
4910 892.008 022475967
10 – 100 μL
4910 893.004 022476009
50 – 200 μL
4910 897.000 022476025
50 – 250 μL
4910 894.000
100 – 1000 μL
4910 895.007 022476084
500 – 2500 μL
4910 896.003 022476108
Reference repair set
4910 805.008 022475045
(Grease for pipettes (1 g), 1 wrench, 6 blank labels,
1 wire punch, 1 filling tube each for 75 – 100 μL,
150 – 200 μL, 250 μL and 300 – 500 μL, 1 damping
tube for 50 – 200 μL)
Calibration aid labels (5 pieces)
Grease for pipettes (1 g)
Calibration seal, red (5 pieces)
Wrench
108
4900 805.000 022475002
0013 063.010 022458507
4910 601.003
4910 092.001 022475029
Eppendorf Reference® – Part B – Ordering information
III
Additional accessories
Pipette carousel, incl. 6 pipette supports
Order number Order number
International North America
3115 000.003 022444905
Pipette holder (replacement for stand)
3115 600.019 022260588
Pipette wall mount (with adhesive surface for
attachment to lab benches and wall)
PICASO II (Pipette Calibration Software)
PICASO accessories
3115 000.020 022444913
3113 004.001
see eppendorf catalog
Operating manual
4910 900.132
109
Eppendorf Reference® – Part B – Ordering information
IV
Pipette tips
epT.I.P.S.
(The packaging units stated represent the minimum ordering quantity).
Length Color code Order number Order number
International North America
Standard, in bags, 2x 500 = 1000 tips
0.1 – 10 μL
34 mm dark gray
0030 000.811 022492004
0.1 – 20 μL
40 mm medium gray 0030 000.838 022492012
0.5 – 20 μL
L
46 mm light gray
0030 000.854 022492021
2
– 200 μL
53 mm yellow
0030 000.870 022492039
50 – 1000 μL
71 mm blue
0030 000.919 022492055
500 tips
500 – 2500 μL
115 mm red
0030 000.951 022492071
Set, 1 Box, incl. 5x 96 tips
0.1 – 10 μL
34 mm
0.1 – 20 μL
40 mm
0.5 – 20 μL
L
46 mm
2
– 200 μL
53 mm
50 – 1000 μL
71 mm
5x 48 tips
500 – 2500 μL
115 mm
Reloads, 10x 96 = 960 tips
0.1 – 10 μL (in stacks) 34 mm
0.1 – 20 μL
40 mm
0.5 – 20 μL
L
46 mm
2
– 200 μL (in stacks)53 mm
50 – 1000 μL
71 mm
10x 48 = 480 tips
500 – 2500 μL
115 mm
dark gray
medium gray
light gray
yellow
blue
0030 073.207
0030 073.223
0030 073.240
0030 073.266
0030 073.304
022491407
022491415
022491423
022491431
022491458
red
0030 073.347
022491474
dark gray
medium gray
light gray
yellow
blue
0030 073.363
0030 073.380
0030 073.401
0030 073.428
0030 073.460
022491504
022491512
022491521
022491539
022491555
red
0030 073.509
022491571
0030 073.746
0030 073.762
0030 073.789
0030 073.800
0030 073.843
022491709
022491717
022491725
022491733
022491750
0030 073.886
022491776
Reloads PCR-clean, 10x 96 = 960 tips
0.1 – 10 μL (in stacks) 34 mm dark gray
0.1 – 20 μL
40 mm medium gray
0.5 – 20 μL
L
46 mm light gray
2
– 200 μL (in stacks)53 mm yellow
50 – 1000 μL
71 mm blue
10x 48 = 480 tips
500 – 2500 μL
115 mm red
110
Eppendorf Reference® – Part B – Ordering information
Length Color code Order number Order number
International North America
Box, 1 box plus 96 tips
0.1 – 10 μL
0.1 – 20 μL
0.5 – 20 μL
L
2
– 200 μL
50 – 1000 μL
48 tips
500 – 2500 μL
34 mm
40 mm
46 mm
53 mm
71 mm
dark gray
medium gray
light gray
yellow
blue
115 mm red
Racks, plus 10x96=960 tips
0.1 – 10 μL
34 mm
0.1 – 20 μL
40 mm
0.5 – 20 μL
L
46 mm
2
– 200 μL
53 mm
50 – 1000 μL
71 mm
480 tips
500 – 2500 μL
115 mm
0030 073.002
0030 073.029
0030 073.045
0030 073.061
0030 073.100
022491300
022491318
022491326
022491334
022491351
0030 073.142
022491377
dark gray
medium gray
light gray
yellow
blue
022491903
022491911
022491920
022491938
022491954
red
022491971
Racks PCR-clean, plus 10x96=960 tips
0.1 – 10 μL
34 mm dark gray
0.1 – 20 μL
40 mm medium gray
0.5 – 20 μL
L
46 mm light gray
2
– 200 μL
53 mm yellow
50 – 1000 μL
71 mm blue
240 tips
500 – 2500 μL
115 mm red
Racks, sterile, plus 10x96=960 tips
0.1 – 20 μL
40 mm medium gray
2
– 200 μL
53 mm yellow
50 – 1000 μL
71 mm blue
Racks Eppendorf Biopur, colorless,
pyrogen-free, DNA-free, RNase-free, ATP-free
5x 96 = 480 tips
0.1 – 20 μL
40 mm medium gray
2
– 200 μL
53 mm yellow
50 – 1000 μL
71 mm blue
240 tips
500 – 2500 μL
115 mm red
022491806
022491814
022491822
022491831
022491857
022491873
022492250
022492276
022492292
0030 075.005
0030 075.021
0030 075.064
022491067
022491083
022491105
0030 075.102
022491121
111
Eppendorf Reference® – Part B – Ordering informations
Length
Color codeOrder number
International
Singles (Eppendorf Biopur), colorless, pyrogen-free,
DNA-free, RNase-free, ATP-free, individually wrapped, 100 tips
0.1 – 20 μL
40 mm
medium
0030 010.019
gray
2
– 200 μL
53 mm
yellow
0030 010.035
50 – 1000 μL
71 mm
blue
0030 010.051
Order number
North America
022491130
022491148
022491156
ep Dualfilter T.I.P.S., PCR-clean, sterile, in racks, 10x 96 = 960 tips
0.1 – 10 μL
S
34 mm
dark gray 0030 077.504 022491202
0.1 – 10 μL
M
40 mm
medium
0030 077.512 022491211
gray
0.5 – 20 μL
L
46 mm
light gray 0030 077.520 022491229
2
– 20 μL
53 mm
yellow
0030 077.539 022491270
2
– 100 μL
53 mm
yellow
0030 077.547 022491237
2
– 200 μL
55 mm
yellow
0030 077.555 022491296
20 – 300 μL
55 mm
orange
0030 077.563 022491245
50 – 1000 μL
76 mm
blue
0030 077.571 022491253
GELoader® tips (f. 0.5 – 10 μL)
1 set = 200 tips
62 mm
112
light gray
0030 001.222
022351656
Eppendorf Reference® – Part B – Ordering informations
Length
Color
code
Order number Order number
International North America
Reloads, LoRetention PCR clean,
10 x 96 = 960 tips
0.1 – 10 μL
34 mm
dark gray 0030 072.006 022493010
0.5 – 20 μL
L
46 mm
light gray 0030 072.014 022493012
2 – 200 μL
53 mm
yellow
0030 072.022 022493014
50 – 1000 μL
71 mm
blue
0030 072.030 022493016
Reloads, LoRetention, autoclavable,
10 x 96 = 960 tips
0.1 – 10 μL
34 mm
dark gray 0030 072.049 022493018
0.5 – 20 μL
L
46 mm
light gray 0030 072.057 022493020
2 – 200 μL
53 mm
yellow
0030 072.065 022493022
50 – 1000 μL
71 mm
blue
0030 072.073 022493024
Racks, LoRetention PCR clean,
10 x 96 = 960 tips
0.1 – 10 μL
34 mm
dark gray
022493026
0.5 – 20 μL
L
46 mm
light gray
022493028
2 – 200 μL
53 mm
yellow
022493030
50 – 1000 μL
71 mm
blue
022493032
Racks, LoRetention Dualfilter, PCR clean, sterile and pyrogen free
10 x 96 = 960 tips
0.1 – 10 μL
S
34 mm
dark gray 0030 077.610 022493000
0.5 – 20 μL
L
46 mm
light gray 0030 077.628 022493002
2 – 100 μL
53 mm
yellow
0030 077.644 022493006
20 – 300 μL
55 mm
orange
0030 077.636 022493004
50 – 1000 μL
76 mm
blue
0030 077.652 022493008
113
Eppendorf Offices
AUSTRALIA & NEW ZEALAND
Eppendorf South Pacific Pty. Ltd.
Phone: +61 2 9889 5000
Fax: +61 2 9889 5111
E-mail: [email protected]
Internet: www.eppendorf.com.au
CANADA
Eppendorf Canada Ltd.
Phone: +1 905 826 5525
Fax: +1 905 826 5424
E-mail: [email protected]
Internet: www.eppendorfna.com
FRANCE
Eppendorf France S.A.R.L.
Phone: +33 1 30 15 67 40
Fax: +33 1 30 15 67 45
E-mail: [email protected]
Internet: www.eppendorf.fr
ITALY
Eppendorf s.r.l.
Phone: +390 2 55 404 1
Fax: +390 2 58 013 438
E-mail: [email protected]
Internet: www.eppendorf.it
SOUTH & SOUTHEAST ASIA
Eppendorf Asia Pacific Sdn. Bhd.
Phone: +60 3 8023 2769
Fax: +60 3 8023 3720
E-mail: [email protected]
Internet: www.eppendorf.com.my
THAILAND
Eppendorf Thailand Co. Ltd.
Phone: +66 2 379 4212-5
Fax: +66 2 379 4216
E-mail: [email protected]
Internet: www.eppendorf.com.my
OTHER COUNTRIES
Internet: www.eppendorf.com/worldwide
AUSTRIA
Eppendorf Austria GmbH
Phone: +43 (0) 1 890 13 64 - 0
Fax: +43 (0) 1 890 13 64 - 20
E-mail: [email protected]
Internet: www.eppendorf.at
CHINA
Eppendorf China Ltd.
Phone: +86 21 38560500
Fax: +86 21 38560555
E-mail: [email protected]
Internet: www.eppendorf.cn
GERMANY
Eppendorf Vertrieb
Deutschland GmbH
Phone: +49 2232 418-0
Fax: +49 2232 418-155
E-mail: [email protected]
Internet: www.eppendorf.de
JAPAN
Eppendorf Co. Ltd.
Phone: +81 3 5825 2363
Fax: +81 3 5825 2365
E-mail: [email protected]
Internet: www.eppendorf.jp
SPAIN
Eppendorf Ibérica S.L.U.
Phone: +34 91 651 76 94
Fax: +34 91 651 81 44
E-mail: [email protected]
Internet: www.eppendorf.es
UNITED KINGDOM
Eppendorf UK Limited
Phone: +44 1223 200 440
Fax: +44 1223 200 441
E-mail: [email protected]
Internet: www.eppendorf.co.uk
BRAZIL
Eppendorf do Brasil Ltda.
Phone: +55 11 30 95 93 44
Fax: +55 11 30 95 93 40
E-mail: [email protected]f.com.br
Internet: www.eppendorf.com.br
CZECH REP. & SLOVAKIA
Eppendorf Czech & Slovakia s.r.o.
Phone: +420 323 605 454
Fax: +420 323 605 454
E-mail: [email protected]
Internet: www.eppendorf.cz /
www.eppendorf.sk
INDIA
Eppendorf India Limited
Phone: +91 44 42 11 13 14
Fax: +91 44 42 18 74 05
E-mail: [email protected]
Internet: www.eppendorf.co.in
NORDIC
Eppendorf Nordic Aps
Phone: +45 70 22 2970
Fax: +45 45 76 7370
E-mail: [email protected]
Internet: www.eppendorf.dk
SWITZERLAND
Vaudaux-Eppendorf AG
Phone: +41 61 482 1414
Fax: +41 61 482 1419
E-mail: [email protected]
Internet: www.eppendorf.ch
USA
Eppendorf North America, Inc.
Phone: +1 516 334 7500
Fax: +1 516 334 7506
E-mail: [email protected]
Internet: www.eppendorfna.com
eppendorf® is a registered trademark.
B 4910 900.159-02/1010 · Printed in Germany · Printed on chlorine-free bleached paper
Your local distributor: www.eppendorf.com/worldwide
Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 538 01-0 · Fax: +49 40 538 01-556 · E-Mail: [email protected]
Eppendorf North America, Inc. · 102 Motor Parkway, Suite 410 · Hauppauge, NY 11788-5178 · USA
Tel: +1 516 334 7500 · Toll free phone: +1 800 645 3050 · Fax: +1 516 334 7506 · E-Mail: [email protected]
Application Support
Europe, International: Tel: +49 1803 666 789 · E-Mail: [email protected]
North America: Tel: +1 800 645 3050, menu option 2 · E-Mail: [email protected]
Asia Pacific: Tel: +60 3 8023 6869 · E-Mail: [email protected]