Touchgene Gradient

Touchgene Gradient
Touchgene
Gradient
OPERATOR’S MANUAL
Issue 3.
Date of issue: 28th November 2001
 Touchgene is the registered trademark of
Techne Incorporated, Princeton, NJ, USA.
 The Techne logo is the trademark of
Techne (Cambridge) Limited and Techne Inc.
© Techne (Cambridge) Limited, 2000
Touchgene
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OPERATOR’S
MANUAL
CONTENTS
Touchgene Gradient Operator’s Manual
page
SAFETY AND INSTALLATION
Declaration of conformity
English
Français
Deutsch
Español
THE TOUCHGENE GRADIENT
Warning
Unpacking
Typical specification at an ambient of 20°C
Working conditions
Uses of the Touchgene
Tubes or reaction vessel
Cleaning your Touchgene Gradient
Block removal
The heated lid
The memory card
Adjusting the light intensity of the screen
Before switching on
Connwctions on the back of the unit
Symbols on the screen
Connecting one unit to the computer using an RS232 Cable
Connecting several units to the computer using the daisy chain cables
Some frequently asked questions
POWER UP SCREENS
System user main menu
Program defaults
Numerical input screen
PROGRAMS
New program screen
Template program screens
Existing program screen
Editing programs
The Gradient
Increment or decrement temperature or time
‘Save’ before ‘Run’
RUNNING A PROGRAM
While running programs
Data logs
Printing Data logs
Using “Gensoft”
ADDITIONAL INFORMATION
Interchangeable blocks
Accessories
Replacement parts
3
4
5
6
7
8
8
8
9
10
10
10
11
11
12
13
13
14
14
14
15
15
16
17
18
18
19
20
20
21
23
24
28
30
31
32
33
34
34
36
37
38
39
39
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Touchgene
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OPERATOR’S
MANUAL
Touchgene
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OPERATOR’S
MANUAL
Techne (Cambridge) Ltd
Duxford
Cambridge
CB2 4PZ
Declaration of Conformity
Techne Unit Touchgene Gradient has been designed to comply with the following European
Standards:
EN 61326:1998 Electrical equipment for measurement, control and laboratory use.
EMC requirements.
EN 61010-1:1993 Safety requirements for electrical equipment for measurement, control
and laboratory use.
EN 61010-2-010:1995 Particular requirements for laboratory equipment for the heating
of materials.
I have made all reasonable enquiries regarding the unit stated and its conformance to the
following EU directives:
Low Voltage directive, 73/23/EEC and amendment 93/68/EEC, and
EMC Directive 89/336/EEC and amendments 91/263/EEC 92/31/EEC and 93/68/EEC.
To the best of my knowledge and belief these units conform to these directives.
ISO
9001
CERTIFIED
This Declaration is controlled under an ISO 9001:1994 system certificated by BSI Quality
Assurance, certificate number FM13585.
Signature
Name
B C Coombes
Position
Quality Manager
Issue 2
27/09/2000
PAGE 3
Touchgene
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OPERATOR’S
MANUAL
Introduction
Please read all the information in this booklet before using the
unit.
Warning
HIGH TEMPERATURES ARE DANGEROUS: they
can cause serious burns to operators and ignite
combustible material.
Techne have taken great care in the design of these
units to protect operators from hazards, but users should pay
attention to the following points:
• USE CARE AND WEAR PROTECTIVE GLOVES TO
PROTECT HANDS;
• DO NOT put hot objects on or near combustible objects;
• DO NOT operate the unit close to inflammable liquids or
gases;
• DO NOT place any liquid directly in your unit;
• At all times USE COMMON SENSE.
Operator Safety
All users of Techne equipment must have available the relevant
literature needed to ensure their safety.
It is important that only suitably trained personnel operate this
equipment, in accordance with the instructions contained in this
manual and with general safety standards and procedures. If the
equipment is used in a manner not specified by Techne the
protection provided by the equipment to the user may be
impaired.
All Techne units have been designed to conform to international
safety requirements and are fitted with an overtemperature cutout. On some models, the cut-out is adjustable and should be set
to suit the application. On all other models the cut-out is preset
to protect the unit.
If a safety problem should be encountered, switch off at the mains
socket and remove the plug from the supply.
Installation
1. All Techne units are supplied with a power cable. This may
be integral or plug-in.
2. Before connecting the mains supply, check the voltage
against the rating plate. Connect the mains cable to a
suitable plug according to the table below. Note that the
unit must be earthed to ensure proper electrical safety.
Connections
220/240V
110/120V
Live
Brown
Black
Neutral
Blue
White
Earth
Green/yellow Green
The fused plug supplied with the mains lead for use in the
UK is fitted with the following value fuse to protect the
cable:
230V UK
10 AMP
The fuse in the unit protects the unit and the operator.
Note that units marked 230V on the rating plate work at
220V; units marked 120V work at 110V. In both cases,
however, the heating rate will degrade by approximately 8%.
The rating plate is on the rear of the unit.
3. Plug the mains cable into the socket on the rear of the unit.
4. Place the unit on a suitable bench or flat workspace, or in a
fume cupboard if required, ensuring that the air inlet vents
on the underside are free from obstruction.
5. Symbols on or near the power switch of the unit have the
following meanings:
I
: mains switch On
O
: mains switch Off
PAGE 4
After use
When you have finished heating samples, remember that parts of
the unit – the tubes, blocks and associated accessories – may be
very hot. Take the precautions listed earlier.
Guarantee
The unit is guaranteed against any defect in material or
workmanship for the period specified on the enclosed guarantee
card. This period is from the date of purchase, and within this
period all defective parts will be replaced free of charge provided
that the defect is not the result of misuse, accident or negligence.
Servicing under this guarantee should be obtained from the
supplier.
Notwithstanding the description and specification(s) of the units
contained in the Operator’s Manual, Techne (Cambridge) Limited
hereby reserves the right to make such changes as it sees fit to the
units or to any component of the units.
This Manual has been prepared solely for the convenience of
Techne (Cambridge) Limited customers and nothing in this
Instruction Book shall be taken as a warranty, condition or
representation concerning the description, merchantability, fitness
for purpose or otherwise of the units or components.
User maintenance
NOTE THAT THIS EQUIMENT SHOULD ONLY BE
DISMANTALED BY PROPERLY TRAINED PERSONNEL.
REMOVING THE SIDE, FRONT OR REAR PANELS EXPOSES
POTENTIALLY LETHAL MAINS VOLTAGES.
THERE ARE NO USER MAINTAINABLE PARTS WITHIN
THE EQUIPMENT.
In the unlikely event that you experience any problems with your
unit which cannot easily be remedied, you should contact your
supplier and return the unit if necessary. Please include any
details of the fault observed and remember to return the unit in its
original packing. Techne accept no responsibilty for damage to
units which are not properly packed for shipping: if in doubt,
contact your supplier. See the De-contamination Certificate
supplied with your unit.
1. Cleaning
Before cleaning your unit ALWAYS disconnect from the
power supply and allow to cool below 50° C.
Your unit can be cleaned by wiping with a damp soapy
cloth. Care should be exercised to prevent water from
running inside the unit. Do not use abrasive cleaners.
2. Fuses
Your unit is protected by one or two fuses. These should
only be changed by suitably qualified personnel.
If the fuses blow persistently, a serious fault is indicated and
you may need to return the unit to your supplier for repair.
Contact Information
For technical, sales or servicing information, contact your local
Techne dealer or,
Techne (Cambridge) Limited, Duxford,
CAMBRIDGE, CB2 4PZ, United Kingdom.
Telephone: +44(0)1223 832401
Telefax:
+44(0)1223 836838
Service:
+44 (0)1223 836950 24 hour answer machine
e-mail:
sales@techneuk.co.uk
Web site:
www.techneuk.co.uk
or,
Techne Incorporated, University Park Plaza,
743 Alexander Road, Princeton, New Jersey,
08540-6328, USA.
Telephone: (609) 452-9275
Toll free:
1-800-225-9243
Telefax:
(609) 987-8177
e-mail:
labproducts@techneusa.com
Web site:
www.techneusa.com
Touchgene
Introduction
Veuillez lire attentivement toutes les instructions de ce document
avant d’utiliser l’appareil.
Avertissement
DANGER DE TEMPERATURES ELEVEES : les
opérateurs peuvent subir de graves brûlures et les
matériaux combustibles risquent de prendre feu.
Techne a apporté un soin tout particulier à la
conception de ces appareils de façon à assurer une protection
maximale des opérateurs, mais il est recommandé aux utilisateurs
de porter une attention spéciale aux points suivants:
• PROCEDER AVEC SOIN ET PORTER DES GANTS POUR
SE PROTEGER LES MAINS.
• NE PAS poser d’objets chauds sur ou près de matériaux
combustibles.
• NE PAS utiliser l’appareil à proximité de liquides ou de gaz
inflammables.
• NE PAS verser de liquide directement dans l’appareil.
• FAIRE TOUJOURS PREUVE DE BON SENS.
Sécurité de l’opérateur
Tous les utilisateurs de produits Techne doivent avoir pris
connaissance des manuels et instructions nécessaires à la garantie
de leur sécurité.
Important : cet appareil doit impérativement être manipulé par un
personnel qualifié et utilisé selon les instructions données dans ce
document, en accord avec les normes et procédures de sécurité
générales. Dans le cas où cet appareil ne serait pas utilisé selon
les consignes précisées par Techne, la protection pour l’utilisateur
ne serait alors plus garantie.
Tous les appareils Techne sont conçus pour répondre aux normes
de sécurité internationales et sont dotés d’un coupe-circuit en cas
d’excès de température. Sur certains modèles, ce coupe-circuit est
réglable pour s’adapter à l’application désirée. Sur d’autres
modèles, il est pré-réglée en usine pour assurer la protection de
l’appareil.
Dans le cas d’un problème de sécurité, coupez l’alimentation
électrique au niveau de la prise murale et enlevez la prise
connectée à l’appareil.
Installation
1. Tous les appareils Techne sont livrés avec un câble
d’alimentation qui peut être intégré à l’appareil ou à
raccorder.
2. Avant de brancher l’appareil, vérifiez la tension requise
indiquée sur la plaque d’identification. Raccordez le câble
électrique à la prise appropriée en vous reportant au tableau
ci-dessous. Il est important que l’appareil soit relié à la
terre pour assurer la protection électrique requise.
Connexions
220/240 V
110/120 V
Phase
Marron
Noir
Neutre
Blue
Blanc
Terre
Vert/juane
Vert
Le fusible à l’intérieur de l’appareil est destiné à assurer la
protection de l’appareil et de l’opérateur.
Remarque : les appareils dont la plaque indique 230 V
peuvent fonctionner sur 220 V, et ceux dont la plaque
indique 120 V peuvent fonctionner sur 110 V. Dans les deux
cas cependant, le capacité de chauffage diminuera d’environ
8 %. La plaque d’identification se trouve à l’arrière de
l’appareil.
3. Raccordez le câble d’alimentation à la prise située à l’arrière
de l’appareil.
4. Placez l’appareil sur un plan de travail ou surface plane, ou
le cas échéant, dans une hotte d’aspiration, en s’assurant que
les trous d’aération situés sous l’appareil ne sont pas
obstrués.
5. Les symboles situés sur ou à côté de l’interrupteur de
l’appareil ont la signification suivante :
I : arrêt
O : marche
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OPERATOR’S
MANUAL
Après utilisation
Lorsque vous avez fini de chauffer les échantillons, n’oubliez pas
que certaines parties de l’appareil - les éprouvettes, leurs supports
et autres accessoires - risquent d’être très chaudes. Il est donc
recommandé de toujours prendre les précautions citées plus haut.
Garantie
L’appareil est garanti contre tout défaut ou vice de fabrication
pour la durée figurant sur la carte de garantie, à compter de la date
d’achat de l’appareil. Au cours de cette période, toutes les pièces
défectueuses seront remplacées gratuitement, dans la mesure où la
défaillance n’est pas due à une mauvaise utilisation, un accident
ou une négligence. Toute réparation sous garantie sera effectuée
par le fournisseur.
Malgré la description et les spécifications de l’appareil données
dans le manuel de l’utilisateur, Techne (Cambridge) Limited se
réserve le droit d’effectuer les changements nécessaires à
l’appareil ou à tout élément qui entre dans sa composition.
Ce manuel a été exclusivement rédigé à l’attention des clients de
Techne (Cambridge) Limited, et aucun élément de ce guide
d’instructions ne peut être utilisé comme garantie, condition ou
représentation concernant la description, commercialisation,
adaptation aux conditions d’utilisation ou autre des appareils ou
de leurs composants.
Entretien utilisateur
IMPORTANT : CET APPAREIL NE PEUT ETRE DEMONTE
QUE PAR DU PERSONNEL QUALIFIE.
LORSQUE LES PANNEAUX AVANT, ARRIERE ET
LATERAUX SONT DEMONTES, L’OPERATEUR EST
EXPOSE A DES TENSIONS QUI PEUVENT ETRE
MORTELLES.
CET APPAREIL NE CONTIENT AUCUN ELEMENT QUI
DEMANDE UN ENTRETIEN DE LA PART DE
L’UTILISATEUR.
Dans le cas peu probable où votre appareil présente un défaut de
fonctionnement auquel il est difficile de remédier, il est alors
préférable de contacter votre fournisseur et, le cas échéant, de
renvoyer le matériel. Veuillez inclure une description détaillée du
problème constaté et retourner l’appareil dans son emballage
d’origine. Techne ne sera pas tenu responsable des dommages
subis par tout appareil dont l’emballage est inadéquat pour le
transport. Pour plus de sûreté, contactez votre fournisseur. Voir le
certificat de décontamination livré avec le produit.
1. Nettoyage
Avant de nettoyer l’appareil, assurez-vous TOUJOURS que
le câble d’alimentation est déconnecté et laissez la
température redescendre en dessous de 50 °C.
Utilisez un chiffon imprégné d’eau savonneuse pour nettoyer
l’appareil. Veillez à ne pas introduire d’eau dans l’appareil.
N’utilisez pas de produits abrasifs.
2. Fusibles
La protection de l’appareil est assurée par un ou deux
fusibles dont le remplacement ne peut être effectué que par
un personnel qualifié.
Si les fusibles sautent sans arrêt, il s’agit d’un problème
sérieux. Nous vous conseillons dans ce cas de prendre
contact avec votre fournisseur pour réparation.
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Touchgene
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OPERATOR’S
MANUAL
Einleitung
Bitte lesen Sie diese Bedienungsanleitung komplett bevor Sie
dieses Gerät benutzen.
Warnung
HOHE TEMPERATUREN SIND GEFÄHRLICH: sie
können dem Bediener ernsthafte Verletzungen
zufügen und brennbare Materialien können sich leicht
entzünden.
Techne hat bei der Konstruktion dieses Gerätes sehr
darauf geachtet, daß der Bediener vor Gefahren geschützt ist.
Dennoch sollten Sie auf die folgenden Punkte achten:
· SEIEN SIE VORSICHTIG UND TRAGEN SIE
SCHUTZHANDSCHUHE
· Legen Sie heiße Gegenstände NICHT auf oder in die Nähe
von leicht brennbaren Materialien; vermeiden Sie Arbeiten
in der Nähe von leicht entzündbaren Flüssigkeiten oder
Gasen.
· Bringen sie KEINE Flüssigkeiten direkt in Ihr Gerät.
· Benutzen Sie immer den normalen Menschenverstand
Sicherheit des Anwenders
Alle Benutzer von Techne Geräten müssen Zugang zu der
entsprechenden Literatur haben, um ihre Sicherheit zu gewähren.
Es ist wichtig, daß diese Geräte nur von entsprechend geschultem
Personal betrieben werden, das die in dieser Gebrauchsanweisung
enthaltenen Maßnahmen und allgemeine
Sicherheitsbestimmungen und -vorkehrungen beachtet. Wenn das
Gerät anders eingesetzt wird als vom Hersteller empfohlen, kann
dies die persönliche Sicherheit des Anwenders beeinträchtigen.
Die Geräte von Techne entsprechen den internationalen
Sicherheitsbestimmungen und sind mit einem automatischen
Übertemperaturabschalter ausgestattet. Bei einigen Modellen ist
der Übertemperaturabschalter verstellbar und sollte je nach
Anwendung entsprechend eingestellt werden. Bei allen anderen
Modellen ist der Temperaturschutz voreingestellt um Schäden am
Gerät zu vermeiden. Wenn ein Sicherheitsproblem auftreten
sollte, muß das Gerät ausgeschaltet und vom Stromnetz getrennt
werden.
Installation
1. Alle Techne Geräte werden mit einem Stromanschlußkabel
geliefert. Dieses ist entweder fest mit dem Gerät verbunden
oder zum Einstecken.
2. Vergleichen Sie, ob die Spannung Ihrer Stromversorgung mit
den Angaben auf dem Typenschild des Geräte
übereinstimmen. Verbinden Sie das Stromanschlußkabel mit
einer geeigneten Stromversorgung gemäß der nächstehenden
Tabelle. Achtung: Das Gerät muß geerdet sein, um die
elektrische Sicherheit zu gewährleisten!
Verbindungen 220/240V
110/120V
Stromführend Braun
Schwarz
Neutral
Blau
Weiß
Erde
Grün/Gelb
Grün
Geräte, die für 230 Volt ausgelegt sind, können auch bei 220
Volt arbeiten, Geräte für 120 Volt auch bei 110 Volt. In
beiden Fällen verringert sich die Aufheizrate um ca. 8%. Das
Typenschild befindet sich hinten am Gerät.
3. Stecken Sie das Stromkabel in die vorgesehene Buchse
hinten am Gerät.
4. Stellen Sie das Gerät auf eine ebene Arbeitsfläche bzw. (falls
erforderlich) unter einen Laborabzug. Beachten Sie, daß die
Entlüftungsrippen an der Geräteunterseite immer frei
zugänglich sind.
5. Die Symbole auf oder neben dem EIN/AUS-Schalter an der
Geräterückseite bedeuten:
I
: An
O
: Aus
PAGE 6
Nach dem Gebrauch
Vergessen Sie nicht, daß Teile des Gerätes (die Gefäße, die
Blöcke und andere Zubehörteile) nach dem Erhitzen von Proben
noch sehr heiß sein können. Bitte beachten Sie die oben
genannten Vorsichtsmaßnahmen.
Garantie
Die Garantiedauer des Gerätes ist auf der beiliegenden
Garantiekarte angegeben und schließt Fehler im Material oder
der Verarbeitung ein. Die Garantiedauer beginnt am Tag des
Einkaufs. Sämtliche defekte Teile werden innerhalb dieses
Zeitraumes kostenlos ersetzt unter der Voraussetzung, daß dem
Defekt keine unsachgemäße Handhabung, Fahrlässigkeit oder ein
Unfall zugrundeliegt. Der unter diese Garantie fallende Service
wird vom Lieferanten geleistet.
Ungeachtet der in dieser Gebrauchsanweisung enthaltenen
Beschreibungen und Spezifikationen, behält sich Techne
(Cambridge) Limited hiermit das Recht vor, Änderungen an den
Geräten bzw. an einzelnen Geräteteilen durchzuführen.
Diese Gebrauchsanleitung wurde ausschließlich dazu erstellt, um
Kunden die Handhabung der Techne-Geräte zu erleichtern.
Nichts in dieser Gebrauchsanleitung darf als Garantie, Bedingung
oder Voraussetzung verstanden werden, sei es die Beschreibung,
Marktgängigkeit, Zweckdienlichkeit oder sonstiges bezüglich der
Geräte oder deren Bestandteile.
Wartung durch den Bediener
BEACHTEN SIE, DASS DIESES GERÄT NUR VON
TECHNISCHEN FACHKRÄFTEN GEÖFFNET UND
DEMONTIERT WERDEN DARF.
DURCH ENTFERNEN DES GEHÄUSES ODER
GEHÄUSETEILEN SIND BAUTEILE MIT
LEBENGEFÄHRLICHEN SPANNUNGEN FREI
ZUGÄNGLICH.
IM INNERN DES GERÄTES BEFINDEN SICH KEINE TEILE,
DIE VOM ANWENDER GEWARTET WERDEN MÜSSEN.
Falls Ihr Gerät nicht ordnungsgemäß arbeitet, wenden Sie sich an
Ihren Lieferanten oder senden Sie das Gerät wenn nötig zurück.
Fügen Sie eine genaue Beschreibung des Defektes bei.
Verpacken Sie das Gerät möglichst im Originalkarton. Bitte
beachten Sie, daß Techne keine Haftung bei Transportschäden
aufgrund unzureichender Verpackung übernnehmen. Setzen Sie
sich im Zweifelsfall mit Ihrem Lieferanten in Verbindung. Bitte
beachten Sie die Entgiftungsbescheinigung, die Sie mit dem
Gerät erhalten haben.
1. Reinigen
Bevor Sie Ihr Gerät reinigen, sollten Sie
•
zuerst den Netzstecker ziehen
•
das Gerät unter 50°C abkühlen lassen.
Ein feuchtes Tuch mit Seifenlösung reinigt Ihr Gerät am
besten. Achten Sie darauf, daß kein Wasser in das Gerät
gelangt. Verwenden Sie keine Scheuermittel.
2. Sicherungen
Die Stromzuleitung ist durch ein oder zwei Sicherungen
geschützt. Diese sollten nur durch qualifiziertes
Fachpersonal ausgetauscht werden. Wenn die Sicherung
wiederholt durchbrennt, liegt ein größerer Defekt vor. Das
Gerät muß zur Reparatur an Ihren Lieferanten eingesandt
werden.
Touchgene
Introducción
Le rogamos lea cuidadosamente la información contenida en este
folleto antes de manipular el aparato.
Aviso
LAS TEMPERATURAS ELEVADAS SON
PELIGROSAS: pueden causarle graves quemaduras y
provocar fuego en materiales combustibles.
Techne ha puesto gran cuidado en el diseño de estos
aparatos para proteger al usuario de cualquier peligro; aún así se
deberá prestar atención a los siguientes puntos:
• EXTREME LAS PRECAUCIONES Y UTILICE GUANTES
PARA PROTEGERSE LAS MANOS;
• NO coloque objetos calientes encima o cerca de objetos
combustibles;
• NO maneje el aparato cerca de líquidos inflamables o gases;
• NO introduzca ningún líquido directamente en el aparato;
• UTILICE EL SENTIDO COMUN en todo momento.
Seguridad del usuario
Todos los usuarios de equipos Techne deben disponer de la
información necesaria para asegurar su seguridad.
De acuerdo con las instrucciones contenidas en este manual y con
las normas y procedimientos generales de seguridad, es muy
importante que sólo personal debidamente capacitado opere estos
aparatos. De no ser así, la protección que el equipo le
proporciona al usuario puede verse reducida.
Todos los equipos Techne han sido diseñados para cumplir con
los requisitos internacionales de seguridad y traen incorporados
un sistema de desconexión en caso de sobretemperatura. En
algunos modelos el sistema de desconexión es variable, lo que le
permite elegir la temperatura según sus necesidades. En otros, el
sistema de desconexión viene ya ajustado para evitar daños en el
equipo.
En caso de que surgiera un problema de seguridad, desconecte el
equipo de la red.
Instalación
1. Todos los aparatos Techne se suministran con un cable de
alimentación. Puede ser fijo o independiente del aparato.
2. Antes de conectarlo, compruebe que el voltaje corresponde
al de la placa indicadora. Conecte el cable de alimentación a
un enchufe adecuado según la tabla expuesta a
continuación. El equipo debe estar conectado a tierra para
garantizar la seguridad eléctrica.
Conexiones
220/240V
110/120V
Linea
Marrón
Negro
Neutro
Azul
Blanco
Tierra
Verde/amarillo Verde
El enchufe suministrado con el cable de alimentación viene
equipado con un fusible del siguiente valor para proteger el
cable:
230V Reino Unido
10AMP
El fusible una vez instalado protege tanto al equipo como al
usuario.
Asegúrese de que los equipos marcados 230V en la placa
indicadora funcionan a 220V y de que los equipos marcados
120V funcionan a 110V. No obstante, en ambos casos la
velocidad de calentamiento se verá reducida en un 8%
aproximadamente. La placa indicadora está situada en la
parte posterior del equipo.
3. Conecte el cable a la toma de tensión en la parte posterior
del equipo.
4. Sitúe el aparato en un lugar apropiado tal como una
superficie de trabajo plana, o si fuera necesario incluso en
una campana con extractor de humos, asegurándose de que
las entradas de aire en la parte inferior no queden
obstruidas.
5. Los símbolos que se encuentran en o cerca del interruptor de
alimentación tienen los siguientes significados:
I
: Interruptor principal encendido
O
: Interruptor principal apagado
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OPERATOR’S
MANUAL
Después de su uso
Cuando haya finalizado el calentamiento de muestras, recuerde
que las piezas del equipo, tales como tubos, bloques y demás
accesorios, pueden estar muy calientes. Tome las precauciones
mencionadas anteriormente.
Garantía
Este aparato está garantizado contra cualquier defecto material o
de fabricación durante el periodo especificado en la tarjeta de
garantía adjunta. Este plazo inicia a partir de la fecha de compra,
y dentro de este periodo todas las piezas defectuosas serán
reemplazadas gratuitamente siempre que el defecto no sea
resultado de un uso incorrecto, accidente o negligencia. Mientras
se encuentre bajo garantía las revisiones las debe llevar a cabo el
proveedor.
A pesar de la descripción y las especificaciones de los aparatos
contenidas en el Manual del Usuario, Techne (Cambridge)
Limited se reserva por medio de este documento el derecho a
efectuar los cambios que estime oportunos tanto en los aparatos
como en cualquier componente de los mismos.
Este manual ha sido preparado exclusivamente para los clientes
de Techne (Cambridge) Limited y nada de lo especificado en este
folleto de instrucciones se tomará como una garantía, condición o
aseveración de la descripción, comerciabilidad o adecuación para
cualquier fin específico de los aparatos o sus componentes.
Mantenimiento
ESTE APARATO DEBE SER DESMONTADO SOLO Y
EXCLUSIVAMENTE POR PERSONAL DEBIDAMENTE
CAPACITADO.
EL RETIRAR LOS PANELES LATERALES, FRONTALES O
TRASEROS SUPONE DEJAR AL DESCUBIERTO TENSION
DE LA RED PELIGROSA.
EL EQUIPO NO CONSTA DE NINGUNA PIEZA DE CUYO
MANTENIMIENTO SE PUEDA ENCARGAR EL USUARIO.
En el caso improbable de que experimentara algún problema con
su aparato que no pudiera resolver con facilidad, debería ponerse
en contacto con su proveedor y devolverlo si fuera necesario.
Indique de forma detallada todos los defectos que haya notado y
devuelva el equipo en su embalaje original. Techne no aceptará
responsabilidad alguna por daños causados en equipos que no
estuvieran debidamente embalados para su envío; si tuviera
alguna duda, póngase en contacto con su proveedor. Sírvase
consultar el Certificado de Descontaminación suministrado con su
aparato.
1. Limpieza
Antes de limpiar su aparato, desconéctelo SIEMPRE de la
fuente de alimentación y permita que se enfríe por debajo de
los 50°C.
Este aparato se puede limpiar pasándole un paño húmedo
enjabonado. Hágalo con cuidado parae evitar que caiga agua
dentro del mismo. No utilice limpiadores abrasivos.
2. Fusibles
Su aparato está protegido por uno o dos fusibles. Sólo deben
cambiarlos personal debidamente capacitado.
Si los fusibles se fundieran repetidamente, esto indicaría una
avería grave y puede que tuviera que devolverle el aparato a
su proveedor para su reparación.
PAGE 7
Touchgene
Gradient
OPERATOR’S
MANUAL
THE TOUCHGENE GRADIENT
Before using the Touchgene Gradient make sure you have read this manual carefully.
If there is any doubt relating to the proper use of this equipment, the staff at Techne or your supplier will
be pleased to assist you.
The Touchgene Gradient provides the researcher with the means of accurately controlling
the temperature profile of samples. It has many scientific applications, including DNA amplification and
sequencing. The Touchgene Gradient can cycle samples between 4°C (39°F) and 99°C (210°F).
The Touchgene Gradient is programmed by means of a touch sensitive LCD graphics
screen. Operator input temperatures are temperatures which you require the sample to achieve.
Displayed temperatures can be the temperatures measured by the block probe or the calculated sample
temperature or both. Profile data is saved in plug in memory cards, the program information is
duplicated with the profile data log, and so the original programs are not required during analysis. A
limited number of programs and all system settings are stored in nonvolatile memory within the
Touchgene Gradient. Additional programs may be stored in the nonvolatile memory cards, which are
plugged into a slot under the cover above the screen.
A programme, which can be recalled from memory, consists of:
• a series of specified temperatures in °C
• the times for which each specified temperature will be held (Hold Times)
• the desired heating or cooling rates, in °C/min, between each specified temperature
• whether the times and/or the temperatures are to increase or decrease when a programme is repeated.
The memory card can store up to 500 three segment programs; the number of programs is
reduced as the number of segments in each program is increased. The maximum is one program of 1500
segments.
Warning !
Please note that the touch screen on the Touchgene Gradient is easily damaged by sharp
objects such as pens, pencils and fingernails. Damage of this sort will be considered as misuse and will
invalidate the guarantee on this component.
Position the unit so that the mains on/off switch is accessable. If a safety problem should be
encountered, switch off at the mains socket and remove the plug from the supply.
If you are using more than one unit in close proximity to each other, there must be at least
100mm between the units to allow the cooling air to flow from them.
Unpacking
When unpacking the unit, check that the following have been removed from the packing:
• The Touchgene Gradient unit with memory card in place
•
Mains cable
• Guarantee card
•
Decontamination Certificate
•
The Touchgene Gradient Operator’s Manual
Make sure you keep the original packing, in case you ever need to return it for service or repair. Techne
accepts no responsibility for damage incurred unless the unit is correctly packed and transported in its
original packing.
PAGE 8
Touchgene
Gradient
OPERATOR’S
MANUAL
Typical Specification at an ambient of 20°C
For a detailed specification please contact Techne Sales
Temperature
Gradient Range
20°C to 70°C
Maximum gradient
30°C
Minimum gradient
1°C
Number of temperature probes
4
Block temperature range
4°C to 99°C
Typical heat up rate
3.0°C/sec
Typical Cool down rate
1.3°C/sec
Block uniformity (without gradient)
< ±0.4°C
Column uniformity with a 10°C gradient
< ±0.3°C
Control accuracy
< ±0.3°C
Gradient accuracy
< ±0.3°C
Temperature set point adjustment
±0.1°C
Integral Heated Lid
The heated lid only comes on if the block temperature is set above 30°C
Selectable heated lid temperature
100°C to 115°C
Heated lid enable/disable
Yes
Warm up time from ambient to 105°C
4 min
Over temperature cutout
Yes
Regulated lid pressure
Yes
Programs
Password protected
Incremental/decremental Segment Hold Time
Incremental/decremental Temperature
Variable programmable ramp rate steps
Programmable ramp rate resolution
Gradient calculator
Maximum programs internal memory
Maximum programs memory card
Program naming capacity
Program interface screen
Pause facility
End of program alarm (can be disabled)
Auto restart on power failure
Run end time calculation
Yes
-99 to +99sec, 1sec intervals
-5.5°C to +5.6°C in 0.1°C intervals
±0.1°C/sec
0.1°C
Yes
50 (dependant upon program complexity)
500 x 3 segment
Alpha/numeric
Touch sensitive
Yes
Yes
Yes
Yes
Miscellaneous
Height x Width x Length
Weight
Power consumption
Parallel port
Serial Port RS232/RS485 adaptable
Connection to PC control program
260 mm x 220 mm x 420 mm
12.5 kg
620W total including the heated lid
Yes
Yes
Yes
PAGE 9
Touchgene
Gradient
OPERATOR’S
MANUAL
Voltage
The units marked 100V will work at any voltage between 90V and 110V.
The units set to 120V will work at any voltage between 110V and 130V.
The units set to 230V will work at any voltage between 210V and 260V.
(See page 14 for setting the voltage.)
However, the performance may not meet the above specification at the extremes of voltage.
Working conditions
The Touchgene Gradient is designed to work safely under the following conditions:
Ambient temperature range
5°C to 40°C
Humidity
Up to 95% relative humidity, non-condensing
Note:
The control specifications are quoted at an ambient temperature of 20°C. The specification
may deteriorate outside an ambient temperature range of 10°C to 30°C.
Radio frequency interference tested and passed to EN50081-1.
Immunity Tested and passed to EN50082-1
Uses of the Touchgene Gradient
The Touchgene Gradient has many scientific laboratory applications, including DNA
sequencing and PCR. Aspects of the PCR process are claimed in U.S. Patent Nos. 4,683,195,
4,683,202, and 4,965,188. Use of the Touchgene Gradient in such processes does not convey a licence
to practice the processes themselves.
Tubes or Reaction Vessel
Techne does not recommend any specific tube or reaction vessel other than those described
in this Manual. We recommend using reaction volumes between 10 and 50µl. The tubes must
withstand a pressure of 1 atmosphere at 100°C. Any vessel must be able to withstand the temperatures
you are using without any danger of them deforming to the point where they fracture.
To test your tubes, put 25µl of water in each of 5 tubes and subject them to a typical
thermal cycling protocol. At the end of the cycle, measure the volume remaining, using a micropipet.
A loss of more than 1 or 2µl indicates a vapour leak.
The amount of volume loss you observe and the change in reactant concentrations you can
tolerate determine the minimum volume that can be used. Typical volume losses of 1µl in 30 cycles
allow the use of samples of 10µl or less.
During the final cooldown, a ring of condensation may form above the liquid level but
below the top of the sample block. This is not usually a cause for concern, as the condensation does not
form during cycling.
PAGE 10
Touchgene
Gradient
OPERATOR’S
MANUAL
Cleaning your Touchgene Gradient
Disconnect your unit from the power supply and allow it to cool to below 50°C before
cleaning it.
The heat/cool block, including wells and flat surfaces, should be cleaned regularly to ensure
optimum heat transfer to the samples. Always clean the block if there has been a spillage. Use a cloth or
cotton buds dipped in a fresh, 50:50 water/isopropanol solution, and make sure that no deposits are left
in the wells.
In the case of radioactive spillages, Techne recommend that you use a proprietary cleaning
agent. Carefully follow the cleaning agent manufacturer’s instructions. The heat/cool block is made of
aluminium. Therefore, an agent such as Neutracon (from Decon Laboratories Ltd.), suitable for
nonferrous metals should be used but remember other parts of the unit are made of ferrous materials and
may be damaged by spillage onto them.
Before using any cleaning or decontamination method except those recommended here, the
responsible body should check with Techne that the proposed method will not damage the equipment.
You can clean the case or the screen of the Touchgene Gradient with a cloth dipped in
water or ethanol (methanol or formaldehyde can also be used). No part of the case or cover should be
immersed in the solvents. Do not use aggressive solvents such as acetone, or abrasive cleaners.
Block removal
The blocks can be removed for a more detailed cleaning.
Ensure that the unit is disconnected from the mains for
removal of block.
Slide the quick release handles in the direction shown
above and lift the assembly out of the unit. Never lift or
carry the block by one quick release handle, always use
both handles or support the block underneath. When
refitting the block, slide the quick release handles in the
opposite direction to the above arrows to lock the block
assembly into the unit.
PAGE 11
Touchgene
Gradient
OPERATOR’S
MANUAL
The Heated Lid
The heated lid is an integral part of the Touchgene Gradient. It can be switched “On” or
“Off”, see ‘Program Defaults’ or ‘Editing screen’.
To open the lid lift the back of the handle as shown and use it to lift the lid. To close the lid
lower the lid onto the block with the samples in and press the handle shut.
To adjust the pressure on the
samples: Adjust the red rotating knob
anticlockwise to give minimal pressure, shut
the lid and latch it. Gently rotate the knob
clockwise until you can just feel pressure
beginning to be applied. Rotate the knob a
further quarter of a turn and this will give
the correct pressure. Do not over-tighten
the knob as this will cause deformation of
the silicone rubber membrane and reduce
the effectiveness of the lid. Once the
pressure has been set it does not need to be
adjusted unless a different block, and hence
a different type of sample holder, is used.
PAGE 12
Touchgene
Gradient
OPERATOR’S
MANUAL
The Memory Card
The Memory Card is inserted
into the slot under the cover at the front of
the unit. Lift the cover and push the
Memory Card into the slot until the button
at the left-hand end of the slot rises. Ensure
that the Memory Card is inserted correctly
by positioning the card so that the ‘front’
label is facing you.
The Memory Card can be used
for storing the programs and data logs of
program runs. The memory card slot also
allows upgrades of the control software.
Either via copying software from a new unit
and transferring it to an older unit, or by
obtaining a copy of the new software from
Techne This is a relatively easy process,
see the “System Administration” card, and
means that you can keep your unit up to
date with the latest software.
Adjusting the Light Intensity of the Screen
The light intensity of the screen can be adjusted to suit various environmental conditions.
Lift the front cover and you will see a small black knob on the left-hand side of the cavity. Rotating this
knob clockwise will make the screen lighter and rotating it anticlockwise will make the screen darker.
Adjust the screen to suit your light conditions.
Adjusting Knob
PAGE 13
Touchgene
Gradient
OPERATOR’S
MANUAL
Before Switching On
∼
∼
115V
For units with Serial Number
-1 to -14 to set the unit to the
correct voltage first remove the
block. From the back of the
unit, look into the space where
the block was and you will see
a label as shown.
230V
Ensure that the voltage selector
switch is set to the appropriate
voltage for your supply.
Units with Serial Number-15
onwards see below.
In the centre of the label is a switch. When the unit leaves the factory the switch is set to 230V to avoid
accidental damage.
Ensure that the appropriate fuses are fitted for your supply.
For 230V set the voltage selector switch set to 230V and fit T6.3A fuses.
For 115V set the switch to 115V and fit T8A fuses.
For 100V units the switch will be set to 115V T10A fuses will be fitted, do not change either of these.
Connections on the back of the unit
Selector switch
RS485 (Multiple
units) or RS232
(Single unit)
Units with Serial Number-15
onwards
Parallel port
For 230V set the voltage
selector switch set to 230V and
fit T6.3A fuses.
RS485 connection
(see next page)
For 115V set the switch to
115V and fit T8A fuses.
Serial port
Selector switch:
“Daisy chain”
(any unit except
last in chain);
“Termination
Box” (last unit in
chain).
Symbols on the Screen
is the symbol for setting the gradient
PAGE 14
230
For 100V the switch will be set
to 115V T10A fuses will be
fitted.
On/Off switch
Ensure that the appropriate
fuses are fitted for your supply.
Touchgene
Gradient
OPERATOR’S
MANUAL
Connecting one unit to the computer using an RS 232 cable
If you are driving one unit only you will need FGEN232which includes; a cable, the
software on disc and an instruction sheet.
Connecting several units to the computer using the daisy chain cables
If you are driving two or more units then you will need FTG485D an RS485 pack set. A
pack set includes: the connection to a computer which also converts to RS485; a 1 metre cables and the
software on disc with an instruction sheet. You will also need one of the following for each unit in the
‘Daisy Chain’:
6103557
Connecting cable.
Notice from the diagram that the cable connections to the Cycler's are alternately bottom to
top on the connector.
Touchgene
Gradient
PAGE 15
Touchgene
Gradient
OPERATOR’S
MANUAL
Some Frequently asked Questions
Q1
What’s the ‘pause function’ (as far as it concerns the before program operation)?
A1
Some users prefer to preheat the heated lid before placing the samples into the unit. The pause
feature is used to stop the unit after the 4 minute heated lid preheat step. It will also sound an
audible alarm indicating that the the machine is ready for the sample tubes or plate to be added.
Pressing ‘unpause’ twice will commence the remaining program.
Q2
Why do you have to press some keys twice to make the unit do what you want it to do.?
A2
With many of the program features there is a fail-safe function which asks if you are sure you
want to perform a particular action. You then have to press a key a second time.
Q3
What is the appropriate pressure of the heated lid? If we do it the way it is described in the
manual the tubes are stuck on the membrane.
A3
If the tubes stick to the lid then this could be because there is some grease or dirt on the rubber
pad. We recommend that the user occasionally dusts the rubber pad with talcum powder to avoid
sticking. It is important not to overtighten the lid a light contact is sufficient.
Q4
What is “Hot start”?
A4
Hot Start is used to make the machine pause after the initial denaturation at a specific temperature,
typically around 70’C. The reason is to allow the addition of TAQ enzyme after the initial 5min
denaturation, this prolongs the half-life and activity of the enzyme.
Q5
What is the incremental function? See pages 30 and 31.
A5
Incremental timing and temperature are used to increase either the time or temperature
incrementally over the 30 cycles.
Incrementation of time is used with ‘Long PCR’ which is when large template fragments are to be
amplified (27KB lambda, 40KB genomic).
Decremental temperature is used for protocols such as ‘Touchdown PCR’ where one starts at a
high anneal temperature and gradually decrease the temperature over the 30 cycles, this ensures a
highly specific product is amplified.
Q6
The way you enter a new programm is not quite understandable.
A6
The logic of this manual is as follows:
a Open programs
1 New programs
2 Templates
3 Existing programs
b Edit programs (including adding segments, loops, etc, to a new program)
c Run programs
Once you are doing what the heading says you remain in that function until you get to another
heading.
Q7
It is not always clear the order that you add parts to the program.
A7
The software will always add a line if you have missed it out. For example if you add a ‘segment’
and you have not added the ‘cycles’ the software will add a line “Number of Cycles 1” above
the segment. You can then edit this line if you require more than one cycle.
If you require further assistance please telephone Techne with the unit in front of you and we can
go through the programming function.
PAGE 16
Touchgene
Gradient
OPERATOR’S
MANUAL
POWER UP SCREENS
The screen is a touch sensitive
LCD graphics screen. Operator
input temperatures are
temperatures which you require
the sample to achieve.
Displayed temperatures are
either the temperatures
measured by the block probe,
the calculated sample
temperature or both.
The screen shown is displayed
when the unit is powered on
and will remain displayed
while the unit performs it’s self
tests.
Techne
Touchgene Gradient
Serial number
GPxxxx/xx
Software version
v.vv
Hardware version
h.hh
Block ID
Grad 96 x 0.2ml
Last system test
dd/mm/yyyy
Copyright protected 2000
Performing self tests
1
2
3
After the self-tests this screen
is displayed unless the unit is
powered up by auto-restart see
a later section.
Fields with full corners are dialogue windows and have
no effect when touched. For example:
Techne
Touchgene Gradient
Fields with cutaway corners are touch sensitive and give
the user some sort of action when touched. For
example:
System
User
System Admin button
This selects administration
functions and is dealt with in
the system administration
section.
System User button
This selects normal user
functions as shown on the next
screen.
Techne
Touchgene Gradient
Serial number
GPxxxx/xx
Software version
v.vv
Hardware version
h.hh
Block ID
Grad 96 x 0.2ml
Last system test
dd/mm/yyyy
Comms address
01
Copyright protected 2000
System
User
System
Admin
PAGE 17
Touchgene
Gradient
OPERATOR’S
MANUAL
System User Main Menu
Programs
By touching this button you
can access all the programs
stored either internally or in the
memory module, see page 18.
Techne
Touchgene Gradient
Program defaults
By touching this button you
can set up the program defaults
for a number of programs. See
below.
Data Logs
By touching this button you
can access the data logs stored
in the plug in memory module.
See a later section of this
manual, pages 32 and 33.
Programs
Data
Logs
Program
defaults
Gradient
Calculator
Exit
Gradient Calculator
See page 26.
Exit
By touching this button you can return to the previous screen.
Program Defaults
When you touch this button you can set up default settings for the programs you are about to put into the
unit.
If you touch the button for a field where there is either ON or OFF then the field will toggle between the
two. For example:
Pause before program
Off
One touch gives:
Pause before program On
A second touch will return the
field to the first example.
If you touch a button where
there is a numerical input it
will transfer you to an input
screen. An example is given
on the following pages.
If you touch the ’&’ down
arrow button the screen will
toggle down to a maximum of
four more fields as shown on
the next screen. You can then
toggle back using the ’%’ up
arrow button.
PAGE 18
Set program Defaults
Heated Lid
Off
Heated Lid before program
Off
Pause before program
Off
Initial denaturation 05m00s
94.0°c
Hot Start
94.0°c
%
&
Exit
Touchgene
Once you have set the defaults
to those required, you return to
the “System User Main Menu”
screen by touching the Exit
button.
Gradient
OPERATOR’S
MANUAL
Set program Defaults
Initial denaturation
05m00s 94.0°c
Hot Start
94.0°c
Final extension
05m00s 72.0°c
Final hold
%
10.0°c
&
either
Exit
or
Numerical Input Screen
An example only is shown. All the screens where a numerical input is required are similar. The ‘h’, ‘m’
and ‘s’ will only appear where the field requires hours, minutes or seconds.
←→
The ’←
→’arrow buttons only operate where you need to change fields.
The ‘Disable’ button puts ‘xxxxx’ in the fields. The button itself will change to ‘Enable’. If the
segment was previously “Disabled” the reverse will happen and you will be able to put numerical values
in the field.
The ‘Cancel’ button takes you back to the previous screen without any changes.
The ‘BS’ button is a back space, if you are at the beginning of a field it will delete the whole field: if you
are in the middle of a field it will delete the previous digit.
Type in the program information; with the units of time where appropriate. The order of the time is not
important, for example if you type in 5s2m the unit will convert it to 02m05s.
Touch ‘OK’ when you have
entered the your program
information.
You will return to the
“Program” screen.
Edit initial denaturation settings
Initial denaturation
95.0°C 05m00s
h
7
8
9
←
→
m
4
5
6
Disable
s
1
2
3
Cancel
0
.
BS
OK
PAGE 19
Touchgene
Gradient
OPERATOR’S
MANUAL
PROGRAMS
Touch the ‘Programs’ button
in “System User Main Menu”
and you will go into the “Select
Program” screen.
How to use “New” programs is
explained below.
Select program
xxxxxx bytes of memory free
b 2 STEP PCR TEMPLATE
b 3 STEP PCR TEMPLATE
An “Existing” program with:
b ICE BUCKET
a “b” prefix is a ‘basic’
b LIGATION 15 DEGREES
program or “Template” (how
i Bill
to use these is explained on
page 20);
%
& either
an “i” prefix means the
program is stored ‘internally’;
New
an “r” prefix means the
or
program is stored on the
memory card or ‘remotely’
(how to edit and use existing programs are explained on page 21).
Exit
Use the, ‘&’or ‘%’, down arrow or up arrow key to toggle through the programs. Select any one by
touching the appropriate field.
If you touch ‘Exit’ you will return to the System User Main Menu.
New Program Screen
If you choose ‘New’ then you will go into an input screen to give the program a name. Once you have
entered the name, you will go into the ‘Editing’ screen.
Touch the ‘&’ down arrow button to toggle more parts of the program. You can then toggle back using
the ‘%’ up arrow button.
‘Del(ete)’ will only be effective
for saved programs.
‘Ins(ert)’ will only be effective
if there is no “Next step” field.
Editing
‘Save As’ will give the
program a different name.
Initial denaturation
‘Save’ will save the program.
Hot start
‘Edit’ will put you into the
“Edit program function”
screen.
Next step
“Your Own”
05m00s 94.0°c
Disabled
Final extension
05m00s 72.0°c
10.0°c
Final hold
‘Exit’ will return you to the
“Program” screen.
‘PWord’ will allow you to
give the program a password so
that other people cannot edit
your program, see page 22
PAGE 20
%
&
Ins
PWord
Save As
Del
Edit
Save
Exit
Touchgene
Gradient
OPERATOR’S
MANUAL
Template Program Screens
Any template program can be
copied and given a new name.
From “Select Program” screen
if you touch “2 step PCR
template” you will come to this
screen.
Touch ‘Copy’ and you will go
into the title screen. Type in
your name for the program and
touch ‘Enter’.
If you try to ‘Edit’ any of these
programs you will find that you
can only “Save as” when you
finish. The ‘Save’ key will not
be on the screen.
If you touch ‘Delete’ you will
be asked if you are sure you
want to delete the program.
Select ‘Yes’ or ‘No’.
Program
Last Modified
01/00/2000 00:00:00
105.0°c
Heated Lid
Heated Lid before program
On
Pause before program
Off
Initial denaturation
05m00s 94.0°c
Hot Start
Disabled
Number Cycles
%
30
&
Copy
Delete
Edit
Print
Exit
Run
Program
2 STEP PCR TEMPLATE
05m00s 94.0°c
Initial denaturation
Before you touch ‘Print’ make
sure that you have a printer
connected to the Touchgene. If
you touch ‘Print’, and you have
a printer connected, it will print
out the program.
Hot Start
If you touch ‘Run’ the unit will
start to run the selected
program. See a later section on
page 30.
Final Hold
The other keys work as for any
other screen.
2 STEP PCR TEMPLATE
Disabled
Number Cycles
30
Seg
MAX°c/m
00m30s
92.0°c
Seg
MAX°c/m
01m00s
68.0°c
Final extension
%
10.0°c
&
Copy
Run
05m00s 72.0°c
Delete
Edit
Print
Exit
Touch the ‘&’ down arrow
button to toggle more parts of
the program.
You can then toggle back using
the ‘%’ up arrow button.
PAGE 21
Touchgene
Gradient
OPERATOR’S
MANUAL
Program
3 STEP PCR TEMPLATE
Hot Start
Disabled
Number Cycles
The 3 step PCR template has
the seg(ments) shown here.
Other settings are as for the 2
step template.
30
Seg
MAX°c/m
00m30s
92.0°c
Seg
MAX°c/m
00m30s
55.0°c
Seg
MAX°c/m
00m30s
72.0°c
05m00s 72.0°c
Final extension
10.0°c
Final Hold
%
&
Copy
Delete
Edit
Print
Exit
Run
Program
ICE BUCKET
Heated Lid
The “Ice Bucket” template has
all fields set to ‘off’ or
‘disabled’ except the final hold
temperature which is set to
4.0°C.
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
Disabled
Final extension
Disabled
4.0°c
Final hold
%
&
Program
LIGATION 15 DEGREES
Heated Lid
The “Ligation 15 degrees”
template has all fields set to
‘off’ or ‘disabled’ except the
final hold temperature which is
set to 15.0°C.
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
Disabled
Final extension
Disabled
Final hold
%
PAGE 22
Disabled
&
15.0°c
Touchgene
Gradient
OPERATOR’S
MANUAL
Existing Program Screen
If you select an existing
program from the “Programs”
screen you will get a screen
similar to this one.
Touch the ‘&’ down arrow
button to toggle more parts of
the program. You can then
toggle back using the ‘%’ up
arrow button.
If you touch ‘Delete’ you will
be asked if you are sure you
want to delete the program.
Select ‘Yes’ or ‘No’.
Before you touch ‘Print’ make
sure that you have a printer
connected to the Touchgene. If
you touch ‘Print’, and you have
a printer connected, it will print
out the program.
If you touch ‘Run’ the unit will
start to run the selected
program. See a later section on
page 30.
In order to edit the program
you must touch the ‘Edit’
button to get into the “Editing”
screen. For editing see the
following pages.
‘Exit’ will return you to the
“System User Main Menu”.
Program
Bill
Last Modified 19/08/2000 14:16:43
Heated Lid
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
05m00s
Final extension
%
94.0°C
Disabled
&
Copy
Run
Program
Delete
Edit
Print
Exit
Bill
Last Modified 19/08/2000 14:16:43
Heated Lid
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
05m00s
Final extension
%
Disabled
&
Copy
Run
94.0°C
Delete
Edit
Print
Exit
PAGE 23
Touchgene
Gradient
OPERATOR’S
MANUAL
Editing Programs
Program
Bill
Last Modified 19/08/2000 14:16:43
Heated Lid
From the “Program” screen
touching ‘Edit’ puts you into a
very similar screen. The top
field will have gone and the
next field will have appeared at
the bottom of the set of seven
fields, as shown in this picture.
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
Number Cycles
%
3
&
Copy
Delete
Edit
Print
Exit
Run
Editing
Bill
Password protection
Initial denaturation
If you do not want other people
editing the program it may be
password protected using
‘PWord’. You will need to
enter it twice to confirm the
password.
Hot Start
Next time you go into
“Editing”, the ‘PWord’ key
will have changed to
Change
PWord
PAGE 24
94.0°c
05m00s
Disabled
05m00s
Number Cycles
72.0°c
3
Seg MAX°C/m
00m30s
92.0°c
Seg MAX°C/m
00m30s
92.0°c
10°c
Next step
Final extension
%
Disabled
&
Ins
PWord
Save As
Del
Edit
Save
Exit
Touchgene
Touch any field you need to edit
and it will become highlighted.
Gradient
Editing
OPERATOR’S
MANUAL
Bill
Heated Lid
Disabled
Switch a function on or off
Heated Lid before program
Off
If the field has “On” or “Off”,
the field will toggle between
“Off” and “On” when you
touch the ‘Edit’ button with the
field highlighted.
Pause before program
Off
Initial denaturation
Disabled
Hot Start
94.0°c
05m00s
Number Cycles
Seg MAX°C/m
%
00m30s
3
92.0°c
10°c
&
Ins
PWord
Save As
Editing
Del
Edit
Save
Exit
Bill
Editing Numerical Values
If the field has a numerical
value or says “Disabled”, you
will get a “Numerical Input”
screen when you touch the
‘Edit’ button with the field
highlighted.
Initial denaturation
Hot Start
Disabled
05m00s
either
72.0°c
Number Cycles
3
Seg MAX°C/m
or
00m30s
55.0°c
Seg MAX°C/m
00m30s
92.0°c
10°c
Next step
Final extension
%
Disabled
&
PWord
Ins
Del
Edit
Save As
Save
then
Exit
PAGE 25
Touchgene
Gradient
OPERATOR’S
MANUAL
Editing
Bill
Initial denaturation
Deleting a Segment
Hot Start
‘Del’ will delete any segment
which is highlighted, but you
will be asked to confirm the
deletion.
Number Cycles
Disabled
3
Seg MAX°C/m
00m30s
55.0°c
Seg MAX°C/m
00m30s
92.0°c
10°c
Next step
Final extension
%
Disabled
&
Ins
PWord
Save As
Del
Edit
Save
Exit
Editing
Bill
Initial denaturation
Hot Start
Disabled
05m00s
Number Cycles
00m30s
55.0°c
Seg MAX°C/m
00m30s
92.0°c
10°c
1
Final extension
%
72.0°c
3
Seg MAX°C/m
Next step
3
72.0°c
05m00s
Disabled
&
Ins
PWord
2 Save As
Del
Edit
Save4
Exit
Inserting Extra Functions
1 Highlight the field after which you wish to add program information, for example the second
“Seg(ment)” in the illustration.
2 Touch ‘Ins(ert)’. A “Next Step” will be added after the highlighted field. (Or you can use the “Next
step” that the program inserts automatically.)
3 Touch the ‘Next Step’ field and highlight it.
4 Touch ‘Edit’. You will now be in the “Edit Program Function” screen.
PAGE 26
Touchgene
Segment
Touch ‘Segment’ to insert a
segment.
Type in the Temperature as
appropriate.
Gradient
OPERATOR’S
MANUAL
Edit program function
Next Step
Segment
h
7
8
9
←
Number
Cycles
m
4
5
6
Continue
Inc
Dec
s
1
2
3
Cancel
0
.
BS
OK
Pause
→
Use the “Right Arrow” key
→’ to move to the Gradient
’→
field.
Type in the Gradient as
appropriate. See page 26.
Use the “Right Arrow” key
→’ to move to the Time field.
’→
Type in the Hold Time as
appropriate.
Use the “Right Arrow” key
→’ to move to the Ramp field.
’→
Type in the Ramp Rate as
appropriate. The Ramp rate
you put in here will be the rate
from the temperature at the
begining of the “Segment” to
the temperature of the midpoint of the “Gradient”. Rates
to the highest and lowest
temperatures will be adjusted
appropriately.
Edit program function
Seg
75.0°c
0°c
00m30s
MAX°c/m
Segment
h
7
8
9
←
Number
Cycles
m
4
5
6
Continue
Inc
Dec
s
1
2
3
Cancel
0
.
BS
OK
Pause
→
PAGE 27
Touchgene
Gradient
OPERATOR’S
MANUAL
The Gradient
The Set Temperature is the temperature that you require of the MIDDLE ROWS of samples in the block.
The GRADIENT is the variation of the two extremes of the samples in the block; the left-hand side
being the coolest and the right-hand side being the hottest.
60°C
Highest temperature
55°C
Set
temperature
50°C
Lowest temperature
1
2
Gradient
10°C
Temperature
The Maximum gradient is 30°C; the lowest cold temperature is 20°C and the hottest temperature is
70°C. However, you must remember that the greater the gradient the poorer the uniformity.
LHS
Centre of Block
RHS
3 4 5 6 7 8 9 10 11 12
Columns
The illustration shows a
Set Temperature of 55°C with a Gradient of 10°C on a 0.2ml x 40 block.
Other examples: Set temperature of 55°C with a gradient of 20°C will give a low of 45°C and a high of
65°C; a set temperatureof 50°C with a gradient of 16°C will give a low of 42°C and a high of 58°C.
Gradient Calculator
While the gradient is substantially linear there are small variations. We have, therefore, included a
gradient calculator to give you a more accurate picture of the gradient.
Touch the ‘Gradient Calculator’ button in “System User Main Menu”, see page 16, and you will go
into the “Gradient Calculator” screen.
An example is shown here but
please note that this is an
idealised picture for this
manual and you must use the
unit screen to get the accurate
picture for your block.
Gradient Calculator
Temperature 55°c
Gradient 10°c
50.6
52.0
53.7
55.2
57.2
58.8
51.2
52.9
54.6
56.2
58.0
59.2
Gradient
Temperature
PAGE 28
Print
Exit
Touchgene
If you want to add other
information, touch ‘Continue’
then the appropriate key
Cycles and/or Pause
Gradient
OPERATOR’S
MANUAL
Edit program function
Next Step
Segment
h
7
8
9
←
Number
Cycles
m
4
5
6
Continue
s
1
2
3
0
.
BS
Then
You can also insert ‘Number
Inc
Dec
of Cycles’, and/or a ‘Pause’ as
appropriate and adjust the
Pause
values to suit.
and/or
→
First
Cancel
OK
Edit program function
First
Last
75.0°c
75.0°c
0°c
0°c
00m30s
00m30s
MAX°c/m
MAX°c/m
Inc/Dec time or temperature
You can insert an incremental
value for Time or Temperature
and these are illustrated on the
next page. Touch ‘Inc/Dec’.
Segment
h
7
8
9
←
Number
Cycles
m
4
5
6
Disable
Inc
Dec
s
1
2
3
Cancel
0
.
BS
Pause
→
OK
PAGE 29
Touchgene
Gradient
OPERATOR’S
MANUAL
Increment or decrement temperature or time.
Under normal circumstances, the Hold Temperature of all segments is constant. However,
it is possible to automatically increment or decrement the temperature of a specified segment of a
programme.
The Hold Temperature of the incremented/decremented segment is the defined Hold
Temperature plus the summation of the increments/decrements. The first cycle is never incremented/
decremented, only subsequent Cycles. If you select a decrement, the Hold Temperature is prevented
from falling below 4°C. If you select an increment, the Hold Temperature is prevented from rising
above 99°C.
Similarly, under normal circumstances, the Hold Time of all segments is constant.
However, it is possible to automatically increment or decrement the duration of a specified segment of a
programme.
The Hold Time of the incremented/decremented segment is the defined Hold Time plus the
summation of the increments/decrements. The first cycle is never incremented/decremented, only
subsequent Cycles. If you select a decrement, the Hold Time is prevented from falling below one
second.
PAGE 30
Touchgene
Gradient
OPERATOR’S
MANUAL
Edit program function
Next Step
Completion
Touch ‘OK’ when you have
the values you need in each of
the fields. You will return to
the “Editing” screen.
Segment
h
7
8
9
←
Number
Cycles
m
4
5
6
Disable
Inc
Dec
s
1
2
3
Cancel
0
.
BS
OK
Pause
→
‘Save’ before ‘Run’
Once you are satisfied that the information is correct you must “Save” the program. Touch ‘Save’.
Alternatively you can give the program a different name by touching ‘Save As’ and following the on
screen instructions.
No Change
Use ‘Exit’ to return to the “Program” screen without editing any information. If you have made changes
it will ask you if you want to save them.
Editing
“Your own”
Heated Lid
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
05m00s
Number Cycles
Seg MAX°c/m
%
72.0°c
3
00m35s
75.0°c
0°c
&
Ins
PWord
Save As
Del
Edit
Save
Exit
PAGE 31
Touchgene
Gradient
OPERATOR’S
MANUAL
RUNNING A PROGRAM
From the “Select program”
screen you will go into the
“Program” screen for the
program selected.
Program
“Your own”
Last Modified 19/08/98 14:16:43
Alternatively, from the
“Editing” screen you will go
back into the “Program” screen
when you ‘Save’ or ‘Save as’.
Heated Lid
From the “Program” screen
when you touch ‘Run’ you
will be asked if you wish to
data log the run.
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
05m00s
Final extension
%
If you answer ‘Yes’ then you
will be asked to give the data
log a name. For printing out
the data log and so on see a
later section on page 32.
72.0°C
Disabled
&
Copy
Run
either
Delete
Edit
Print
Exit
or
Typical Program Printout
From the “Program” screen you
can print the program by
touching the ‘Print’ key. A
typical print-out is shown
below.
TOUCHgene program
02/12/98 08:29:44
System Version
TOUCHgene Serial Number
Program
Date last edited
:0.49 1.00
: 88192/01
: DEPT PROGRAM
: 12/10/1998 22:48:11
Auto Restart
Password Protection
Heated Lid
Initial Denaturation
Hot Start
: Enabled
: Disabled
: Enabled
: Disabled
: Disabled
01 Cycles
Segment
03 Cycles
Segment
Segment
Segment
PAGE 32
Set Point°C
4.0
RampRate°C
Max
HoldTime
00:10:00
Set Point°C
95.0
55.0
72.0
RampRate°C
Max
Max
Max
HoldTime
00:00:30
00:00:30
00:01:00
Touchgene
Gradient
OPERATOR’S
MANUAL
While Running Programs
As the program progresses you will eventually get a screen similar to this diagram. The trace is plotted
from the right-hand side and so initially you will have a blank graph with two axis lines. The trace will
consist of one or two lines, the block
temperature, the sample
Program
“Your own”
temperature or both, this
Data log None
depends on the selection you
Cycle 1 of 6
step 5 of 8
Holding
at
55.0°c
10.0°c
made in “Program Defaults”.
If you wish to pause the
program touch the ‘Pause’
button. Press ‘Unpause’ and
the program will continue.
If you wish to stop the
program, you have to press
‘Stop’ twice. This is to prevent
accidental interruption of the
program. The fields at the
bottom of the screen will
change to ‘Run’ and ‘Exit’.
Touching ‘Run’ will start the
program from the beginning
again. Touching ‘Exit’ will
take you back to the program
screen.
Sample Lw =
50.0°c, Sample Up
Time to complete 01m26s
= 60.0°c
100
50
0
00h01m
Stop
00h02m
00h03m
00h04m
00h05m
Pause
PAGE 33
Touchgene
Gradient
OPERATOR’S
MANUAL
Data Logs
Once a program has run and the
data has been logged, it can be
printed and/or deleted from the
“System User Main Menu”.
Touch ‘Data logs’ and you will
get the screen shown here.
Select either ‘Print data log’
or ‘Delete data log’. If you
select print ensure that you first
have a printer connected.
‘Exit’ will return you to the
Main Menu.
Select data log
i Bill
i Bob
i Dick
“Your own”
%
&
Print
data log
Delete
data log
Exit
Printing Data Logs
As the Touchgene Gradient runs, profile information is stored on the Memory Card. This
information defines the times and temperatures at various points during the run. A range of events
creates data which is used to create a profile of the run.
If you require a printed output give the data log a name when requested, the data will then
be stored under that name while the program is running. Once the program is complete, from the
“System User” screen choose ‘Data Logs’. Select the Data Log that you want to print by highlighting it.
Make sure that the printer is plugged in and set on line before pressing the print key. Note that profiles
cannot be printed out during calibration.
The events which are used to generate profile information are:
PAGE 34
Start
A start of program event is generated on first pressing the run key.
Stop
A subsequent press of the stop key stops a run prematurely. This
event generates a printer form feed.
Start of program
Whenever a new program starts. This event generates a Start of cycle
event.
Start of cycle
Whenever the current program starts a new cycle. This event
generates a Start of Segment event.
Start of segment
Whenever a new segment starts.
Start of Hold Time
Whenever the current segment reaches the Hold Time window.
End of program
Whenever the run ends due to the program finishing. This event
generates a printer form feed.
Pause on
Whenever the pause key is first pressed to pause a running program.
Pause off
Whenever the unpause key is subsequently pressed to end a pause
period.
Touchgene
Gradient
OPERATOR’S
MANUAL
A printed profile is 80 columns wide and uses only standard characters in the ASCII range 1 to 127: thus
all common dot matrix, pin fed, continuous stationery printers can be used.
An example of a typical printed profile is shown here:
Note the following:
• The second line shows the block type detected and the software version number.
• At the start of each cycle, the cycle number is shown.
• The start time is the time according to the real time clock in the unit.
• The Rate is prefixed with either a ‘+’ or ‘-’ to indicate a heating (+) or cooling (-) segment.
Typical Data Log Printout
TOUCHgene program
30/11/98
System Configuration.
System Version
:
Unit Serial No.
:
Last System test
:
Program
Date last edited
Data Log File
:
:
:
Event
Date & Time
Program
Ramping
Holding
Ramping
Holding
Ramping
Holding
Ramping
Holding
Ramping
Holding
14:27:25
0.49 1.00
88192/01
19/11/1998 09:34:24
DEPT PROGRAM
12/10/1998 22:46:11
NORMAN
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
14:27:26
14:27:26
14:27:51
14:37:52
14:38:29
14:38:59
14:39:26
14:39:56
14:40:05
14:41:05
14:41:16
Func
Cycle Step
Temp
01
01
02
02
02
02
02
02
02
02
01
01
01
01
01
01
01
01
02
02
4.0°C
6.3°C
4.0°C
88.1°C
95.0°C
59.2°C
55.0°C
65.5°C
72.0°C
88.5°C
01
01
01
01
02
02
03
03
01
01
Set
Point
Ramp
Rate
4.0°C
4.0°C
95.0°C
95.0°C
55.0°C
55.0°C
72.0°C
72.0°C
95.0°C
95.0°C
MAX °C/m
MAX °C/m
MAX °C/m
MAX °C/m
MAX °C/m
Signed:
Typical Graph from a Data Log
Data Log
:
Program Name :
Time Started :
0
NORMAN
DEPT PROGRAM
30/11/1998 14:27:26
10
20
Temperature (°C)
30
40
50
60
70
80
90
Time (m) 0
5
10
20
PAGE 35
Touchgene
Gradient
OPERATOR’S
MANUAL
Using Gensoft
The “Gensoft” software package is available to enable you to connect the Touchgene Gradient to a
computer via a ‘Comms’ port. “Gensoft” offers:
A Daisy chaining a number of units together to a maximum of 32 units
B Creation of thermal cycling protocols through as easy to use Microsoft Windows user interface and
transmitting them to one or more units.
C Selection of units to start and stop a program
D Diagrammatic status of all units connected, including information on the current position in the
protocol of each individual unit and the time remaining.
F Print out of the protocol in a diagrammatic format together with associated notes page.
If you are driving one unit only you will need:
FGEN232
An RS232 cable set which includes; a cable, the software on disc and an instruction
sheet.
If you are driving two or more units then you will need:
FTGEN485
A Computer Connection set.
This set includes: the connection to a computer; the necessary cables; and the software
on disc with an instruction sheet. There are two versions of the software, one which
drives up to 5 units and is faster than the version that will drive up to 32 units.
and the following:
6103557
A cable to connect the units together, one for each unit.
Contact your dealer or Techne Sales for a full list of parts required for your application.
PAGE 36
Touchgene
Gradient
OPERATOR’S
MANUAL
ADDITIONAL INFORMATION
Brief fault finding notes and a list of replacement parts are given in this section.
Note that this equipment should only be dismantled by properly trained personnel. Removing the outer
cover exposes potentially lethal mains voltages.
There are no user serviceable parts within this equipment.
Fault Finding
Should you have any problems with your Touchgene Gradient which cannot be easily remedied, you
should contact your supplier and return the unit if necessary. Please include details of the fault observed
and remember to return the unit in its original packing. Techne accept no responsibility for damage to
units which are not properly packed for shipping: if in doubt, contact your supplier, giving the full serial
number of the unit and software version number (shown when the unit is first switched on).
Fuses
If neither the power light nor displays on the front panel are lit, one of the two fuses may have blown.
Check that there is no external cause, such as a faulty plug or lead. Check both fuses and replace the
faulty fuse with a new one of the correct value. (Fuse values are given on the label next to the power
inlet.) Note that fuses should only be replaced by a qualified electrician.
The holder for the two fuses is built into the mains input socket. First remove the power cable, then
gently prise the fuse drawer open with a flat-bladed screwdriver or similar tool.
Each fuse can be removed by using the screwdriver as a lever.
Exchange the faulty fuse in the fuse holder for a working fuse of the correct value. Finally, replace the
fuse drawer in the fuse compartment and push the drawer shut.
Fuses which blow repeatedly indicate a serious fault and you should return the unit to your supplier for
repair.
Insulation Testing
This equipment is fitted with RFI suppression circuitry. Any check of the electrical insulation by means
of high voltage dielectric testing (for example as in BS EN 61010-1) must be carried out using only a
DC voltage.
This unit contains semiconductor components which may be damaged by electric field effects.
PAGE 37
Touchgene
Gradient
OPERATOR’S
MANUAL
Interchangeable Blocks
Block removal
Ensure that the unit is turned
off and disconnected from
mains.
The block can be removed and
exchanged for another as
required.
Slide the quick release handles
in the direction shown above
and lift the assembly out of the
unit.
Never lift or carry the block by
one quick release handle,
always use both handles or
support the block underneath.
When refitting the block, or fitting a new one, slide the quick release handles in the opposite direction to
the above arrows to lock the block assembly into the unit. The software will register the change and
calibrate the unit for the new block.
Remember to adjust the options in the Program Defaults Menu for the new application.
If you have fitted the flat block for slide applications you must choose Heated Lid OFF. See the section
headed Program Defaults Menu.
PAGE 38
Touchgene
Gradient
OPERATOR’S
MANUAL
Accessories
The following accessories can be obtained from Techne or your Techne dealer:
Item No
Description
Quantity
FTU05TW 0.5ml microtubes, thin wall
Pack of 1000 with caps
FTU02TW 0.2ml microtubes, thin wall
Pack of 1000 with caps
FMITUBC 0.2ml microtubes, thin wall
125 strips of 8
FMICAPC 0.2ml micro tube caps
125 strips of 8
F96T02
96 well plate, flat top non-skirted, pad of 25 plates
pack of 25
F96PLNS
96-well plate raised top non-skirted
Case of 50
F96PLHS
96-well plate half skirt
Case of 50
F96SEAL
96-well sealing mat (silicone)
Case of 50
FGRAD02 Gradient block 96 x 0.2ml microtube or 96 well plate
1
FGRAD05 Gradient block 40 x 0.5ml microtube
1
FGRADF
1
Flat plate block for in situ (non gradient)
For full details of “Gensoft” contact your dealer or Techne Sales Department.
Replacement parts
The following replacement parts can be obtained from Techne or your Techne dealer:
Item Nº
Description
Quantity required
FCABRTUK
Mains cable and plug UK 230V
1
FCABRTEU
Mains cable and Schuko plug 230V
1
FCABRTUS
Mains cable and US 3-pin plug 120V
1
FCABRTUS
Mains cable and US 3-pin plug 100V
1
6500342
Fuse T6.3A, 230V units
2
6008445
Fuse T8A, 120V units
2
6500324
Fuse T10A, 100V units
2
6104172
Non-Volatile Memory Module
1
PAGE 39
Touchgene
Gradient
OPERATOR’S
MANUAL
Notes
PAGE 40
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