Professional pH and Temperature
Meter for Food Products
INSTRUCTION MANUAL
HI98161
Dear
Customer,
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the instrument.
This manual will provide you with the necessary information for correct use of the
instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail us at
tech@hannainst.com or view our worldwide contact list at www.hannainst.com.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of
the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
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TABLE OF CONTENTS
PRELIMINARY EXAMINATION ..............................................................................................
SAFETY MEASURES ............................................................................................................
GENERAL DESCRIPTION ......................................................................................................
FUNCTIONAL DESCRIPTION ................................................................................................
SPECIFICATIONS ................................................................................................................
OPERATIONAL GUIDE .........................................................................................................
pH CALIBRATION ...............................................................................................................
pH BUFFER TEMPERATURE DEPENDENCE ............................................................................
GOOD LABORATORY PRACTICE (GLP) ...................................................................................
SETUP ..............................................................................................................................
LOGGING ..........................................................................................................................
AUTOEND .........................................................................................................................
TEMPERATURE CALIBRATION (FOR TECHNICAL PERSONNEL ONLY) .........................................
PC INTERFACE ...................................................................................................................
BATTERIES REPLACEMENT ..................................................................................................
TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS .......................................................
ELECTRODE CONDITIONING AND MAINTENANCE ..................................................................
TROUBLESHOOTING GUIDE ................................................................................................
ACCESSORIES ...................................................................................................................
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PRELIMINARY EXAMINATION
Remove the instrument from the packing material and examine it carefully to make sure that no
damage has occurred during shipping. If there is any damage, please contact your local Hanna
Instruments Office.
Each instrument is supplied with:
•FC2023 PVDF body, pH probe with internal temperature sensor
•HI700641 Electrode cleaning solution for dairy products (food industry) (2 pcs.)
•HI92000 PC Software
•HI920015 USB cable
•pH 4.01 & 7.01 Buffer solutions (230 mL each)
•100 mL Beaker (2 pcs.)
•1.5V AA Batteries (4 pcs.)
•HI720161 Hard carrying case
•Instruction Manual and Quick Reference Guide
•Instrument Quality Certificate
SAFETY MEASURES
Note: Save all packing material until you are sure that the instrument functions correctly. All
defective items must be returned in the original packing with the supplied accessories.
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Before using this product, make sure that it is entirely suitable for your specific application and for
the environment in which it is used.
Operation of this instrument may cause interference to other electronic equipment, requiring the
operator to take steps to correct interference. Any variation introduced by the user to the supplied
equipment may degrade the instrument’s EMC performance.
To avoid damages or burns, do not put the instrument in microwave ovens. For your and the
instrument’s safety, do not use or store the instrument in hazardous environments.
GENERAL DESCRIPTION
HI98161 is a portable, pH and temperature meter specifically designed for the Food sector.
It is provided with a series of new diagnostic features which add an entirely new dimension to the
measurement of pH, by allowing the user to dramatically improve the reliability of the measurement:
• Seven standard buffers (pH 1.68, 4.01, 6.86, 7.01, 9.18, 10.01 and 12.45) for calibration.
• pH calibration up to five calibration points (see instrument specifications).
• Custom calibration with up to five custom buffers.
• Messages on the graphic LCD for an easy and accurate calibration.
• Cal Check™ Diagnostic features to alert the user when the electrode needs cleaning.
• Optional user enabled “Outside Calibration Range” warning.
• Monitoring of the electrode aging.
• User selectable “Calibration Time Out” to remind when a new calibration is necessary.
This meter is supplied with a pH electrode specifically designed for use with food products. The
FC2023 pH electrode with a built-in temperature sensor features a rugged easy to clean PVDF body
with a strong conic tip ideal for measurements in semisolid such as meats and cheeses. The electrode
also features an open reference junction design that resists clogging from the solids found in food
products.
Other features include:
• Log on demand up to 200 samples (100 pH and 100 mV).
• Auto Hold feature, to freeze first stable reading on the LCD.
• GLP feature, to view last calibration data for pH.
• PC interface.
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FUNCTIONAL DESCRIPTION
FRONT VIEW
1) Liquid Crystal Display (LCD).
2) Functional keys.
3) / keys to scroll through calibration buffers, setup options, manual temperature compensation
and logged data.
4) ON/OFF ( ) key, to turn the instrument ON and OFF.
5) LIGHT ( ) key to toggle display backlighting.
6) GLP key, to display Good Laboratory Practice information.
7) CAL key, to enter/exit calibration mode.
8) SETUP key, to enter/exit SETUP mode.
9) RCL key, to enter/exit view logged data mode.
10)MODE key to change pH resolution.
11)RANGE key, to switch between pH and mV range.
12)HELP key to enter/exit contextual help.
13)ESC to leave current mode, exit calibration, setup, help. etc.
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FUNCTIONAL DESCRIPTION
TOP VIEW
14)Electrode quick connect DIN connector.
15)USB connector.
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SPECIFICATIONS
pH
Temperature
mV
Range
Resolution
Accuracy
Range
Resolution
Accuracy
-2.0 to 20.0 pH / -2.00 to 20.00 pH / -2.000 to 20.000 pH
0.1 pH / 0.01 pH / 0.001 pH
±0.1 pH / ±0.01 pH / ±0.002 pH
-20.0 to 120.0 °C (-4.0 to 248.0 °F)
0.1 °C (0.1 °F)
±0.4 °C (±0.8 °F) (excluding probe error)
Range
±2000.0 mV
Accuracy
0.1 mV
Resolution
±0.2 mV
pH Calibration
Slope Calibration
Temperature Compensation
pH Electrode
LOG
Input Impedance
Battery Type/Life
Auto Power Off
PC Interface
Dimensions
Weight
Environment
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Up to five point calibration, seven standard buffers available
(pH 1.68, 4.01, 6.86, 7.01, 9.18, 10.01, 12.45), and five custom
buffers
From 80 to 110%
Automatic
FC2023 pH & temperature
On demand, 200 samples (100 pH and 100 mV)
10¹² Ω
1.5V AA batteries (4 pcs.) / approx. 200 hours of continuous use
without backlight (50 hours with backlight)
User selectable: 5, 10, 30, 60 minutes or disabled
opto-isolated USB
185 x 93 x 35.2 mm (7.3 x 3.6 x 1.4”)
400 g (14.2 oz)
0 to 50 °C (32 to 122 °F) max. RH 100% IP67
OPERATIONAL GUIDE
INITIAL PREPARATION
The instrument is supplied complete with 1.5V AA (4 pcs.) batteries. For placing the batteries inside
the meter, see page 42.
Make sure that the protective micro USB cover is used when not connected to a computer to ensure
waterproof protection.
For HI98161 connect the pH/temperature electrode to the DIN connector.
Turn the instrument ON by pressing ON/OFF key.
At start-up the display will show the Hanna Instruments logo for a few seconds followed by the
percentage indication of the remaining battery life, then enters the measurement mode.
After measurement switch the instrument off, clean the electrode and store it with a few drops of
HI70300 storage solution in the protective cap (see page 45).
The Auto Power Off feature turns the instrument off after a set period (default 30 min) with no button
pressed to save battery life. To set another period or to disable this feature, see SETUP menu on
page 23.
The Auto Light Off backlight feature turns the backlight off after a set period (default 1 min) with
no buttons pressed. To set another period or to disable this feature, see SETUP menu on page 23.
pH MEASUREMENTS
To take a pH measurement remove the electrode protective cap and simply submerge the tip of the
electrode (4 cm/1½") into the sample to be tested.
Press RANGE key to choose between pH and mV ranges.
Use MODE key to select the pH resolution.
Allow time for the electrode to adjust and reading to stabilize (hourglass symbol turns off).
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OPERATIONAL GUIDE
On the pH screen are displayed:
• pH reading with the selected resolution.
• Temperature reading in the selected unit (°C or °F).
• Electrode condition during the calibration day.
• The buffers used in last pH calibration (if feature is enabled in SETUP).
• Battery level indicator.
• Available functional keys.
In order to take more accurate pH measurements, make sure that the instrument is calibrated (see
page 11 for calibration details).
It is recommended that the electrode is always kept moist and rinsed thoroughly with the sample to
be measured before use.
The pH reading is directly affected by temperature. For accurate pH measurements, temperature
must be taken into consideration. If the sample temperature is different from the temperature at
which the pH electrode was kept, allow a few minutes to reach thermal equilibrium.
TEMPERATURE MEASUREMENTS
For HI98161 the temperature sensor is connected through DIN socket.
Note: The temperature can be displayed in Celsius degrees (ºC) or in Fahrenheit degrees
(ºF) (see SETUP for details, page 23).
BACKLIGHT FEATURE
The instrument is provided with a Backlight feature, which can be easily toggled on and off through
the keyboard by pressing LIGHT.
Note: The backlight automatically shuts off after a set period (see SETUP for details,
page 23) with no buttons pressed.
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pH CALIBRATION
It is recommended to calibrate the instrument frequently, especially if high accuracy is required.
The pH range should be recalibrated:
• Whenever the pH electrode is replaced.
• At least once a week.
• After testing aggressive chemicals.
• When calibration alarm time out is expired - “CAL DUE” blinks (if feature is enabled in
SETUP).
• If “Outside Cal Range” message blinks during pH measurement (the measurement range is
not covered by current calibration, if feature is enabled in SETUP).
PROCEDURE
HI98161 instrument offers a choice of seven standard buffers (pH 1.68, 4.01, 6.86, 7.01, 9.18, 10.01
and 12.45). The meter allow the user to set up to five custom buffers. The custom buffers are the
buffer values at 25 °C.
When a custom buffer is selected during calibration, the Custom functional key is displayed on the
LCD. Press Custom key in order to enter custom buffer changing mode. Use / keys to change
the value in a ±1.00 pH window, in according with the temperature reading and then Accept. Press
ESC to leave custom buffers value unchanged.
For accurate pH measurements, it is recommended to perform a calibration with buffers that bracket
the expected pH value. At least, two point calibration is recommended.
The instrument will automatically skip the buffers used during calibration and the buffers which are
in a ±0.2 pH window around one of the calibrated buffers.
• Remove protective cap and rinse the electrode with distilled or deionized water.
• Pour small quantities of selected buffers solutions into clean beakers. For accurate calibration use
two beakers for each buffer solution, the first one for rinsing the electrode and the second one
for calibration.
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pH CALIBRATION
FIVE POINT CALIBRATION
• Immerse the pH electrode approximately 4 cm (1½”) into a buffer solution of your choice
(pH 1.68, 4.01, 6.86, 7.01, 9.18, 10.01, 12.45 or a custom buffer) and stir gently.
• Press CAL. The instrument will display the measured pH, the LCD first expected buffer and the
temperature reading.
• If necessary, press the / keys to select a different buffer value.
• The “ ” tag will blink on the LCD until the reading is stable.
• When the reading is stable and within range of the selected buffer, CFM functional key is displayed.
• Press CFM to confirm first point.
• The calibrated value and the second expected buffer value is then displayed on the LCD.
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pH CALIBRATION
• After the first calibration point is confirmed, immerse the pH electrode probe approximately
4 cm (1½”) into the second buffer solution and stir gently.
• If necessary, press the / keys to select a different buffer value.
• The “ ” tag will blink on the LCD until the reading is stable.
• When the reading is stable and within range of the selected buffer, the CFM functional key is
displayed.
• Press CFM to confirm calibration.
• The calibrated value and the third expected buffer value will be displayed.
• After the second calibration point is confirmed, immerse the pH electrode approximately
4 cm (1½”) into the third buffer solution and stir gently.
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pH CALIBRATION
• If necessary, press the / keys to select a different buffer value.
• The “ ” tag will blink on the LCD until the reading is stable.
• When the reading is stable and within range of the selected buffer, the CFM functional key is
displayed.
• Press CFM to confirm calibration.
• The calibrated value and the fourth expected value will be displayed.
• After the third calibration point is confirmed, immerse the pH electrode approximately
4 cm (1½”) into the fourth buffer solution and stir gently.
• If necessary, press the / keys to select a different buffer value.
• The “ ” tag will blink on the LCD until the reading is stable.
• When the reading is stable and within range of the selected buffer, the CFM functional key is
displayed.
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• The calibrated value and the fifth expected buffer will be displayed.
• After the fourth calibration point is confirmed, immerse the pH electrode approximately
4 cm (1½”) into the fifth buffer solution and stir gently.
pH CALIBRATION
• Press CFM to confirm calibration.
• If necessary, press the / keys to select a different buffer value.
• The “ ” tag will blink on the LCD until the reading is stable.
• When the reading is stable and within range of the selected buffer, the CFM functional key is
displayed.
• Press CFM to confirm calibration.
• The instrument stores the calibration values and returns to normal measurement mode.
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pH CALIBRATION
FOUR, THREE, TWO or ONE POINT CALIBRATION
• Proceed as described in “FIVE POINT CALIBRATION” section.
• Press CAL or ESC after the appropriate accepted calibration point. The instruments will return to
measurement mode and will memorize the calibration data.
FIRST POINT MODE
Two SETUP options are available to determine how the previous calibration will be affected by a
subsequent one point calibration. These options are replace or offset.
If the Replace option is selected, the slopes between current buffer and nearest lower and higher
buffers will be recalculated.
If the Offset option is selected, an electrode offset correction is performed keeping unchanged the
existing slopes.
ERROR SCREENS
Wrong buffer
The calibration cannot be confirmed.
The pH reading is not within range of the selected buffer. Select another buffer using the / keys
or change the buffer.
Electrode Dirty/Broken alternatively with Buffer Contaminated
The calibration cannot be confirmed.
The offset of the electrode is not in the accepted range. Check if the electrode is broken or clean it
following the Cleaning Procedure (see page 45). Check the quality of the buffer. If necessary, change
the buffer.
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The evaluated slope is less than the lowest accepted value (80% of default slope).
pH CALIBRATION
Wrong slope
The calibration cannot be confirmed.
The evaluated slope is more than the highest accepted value (110 % of default slope).
Wrong old slope
An inconsistency between new and previous (old) calibration is detected. Clear old calibration
parameters and proceed with the calibration from the current point. The instrument will keep all
confirmed values during current calibration.
Note: For one point calibration the electrode condition is not displayed in the measurement
screen. Each time a buffer is confirmed, the new calibration parameters replace the old
calibration parameters of the corresponding buffer.
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pH CALIBRATION
If the current confirmed buffer has no correspondence in the existing stored calibration and this is
not full, the current buffer is added to the existing stored calibration.
If the existing stored calibration is full (five calibration points), after confirming the calibration point,
the instrument will ask which buffer will be replaced by current buffer.
Press / keys to select another buffer to be replaced.
Press CFM to confirm the buffer that will be replaced.
Press CAL or ESC to leave replace mode. In this case, the buffer will not be memorized.
Note: The replaced buffer is not removed from calibration list and it can be selected for the
next calibration points.
WORKING WITH CUSTOM BUFFERS
If at least one custom buffer was set in SETUP menu, it can be selected for calibration by pressing
the / keys. The Custom functional key will be displayed.
Press Custom if you want to adjust the buffer value according with current temperature.
Use the / keys to change the buffer value.
Press Accept to accept new value or ESC to exit changing mode.
Note: Custom buffer value can be adjusted in a ±1.00 pH window, around the set value.
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pH CALIBRATION
WORKING WITH MILI pH BUFFERS
If calibration is invoked from mili pH range, the calibration buffer can be modified in a ±0.020 pH
range in according with the label on the calibration buffer.
Press Change to enter buffer adjust mode.
Use / keys to change the buffer value.
Press Accept to accept new value or ESC to exit adjusting mode.
CLEAR CALIBRATION
Press Clear functional key when displayed to clear old calibrations.
All old calibrations, are cleared and the instrument continues calibration. The points confirmed in
current calibration are kept.
Note: If Clear calibration is invoked during the first calibration point, the instrument returns
to measurement mode.
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pH CALIBRATION
ELECTRODE CONDITION
The display is provided with an icon, and a numeric value (unless the feature is disabled) which gives
an indication of the electrode status after calibration.
The “condition” remains active until the end of the calibration day.
Note: The electrode condition is evaluated only if current calibration includes at least two
standard buffers.
CLEAN ELECTRODE WARNING
Each time pH calibration is performed, the instrument internally compares the new calibration with
the one previously stored.
When this comparison indicates a significant difference, the “Clean Electrode” warning message is
displayed to advise the user that the pH electrode may need to be cleaned (see ELECTRODE
CONDITIONING AND MAINTENANCE section for details, page 44).
After cleaning, perform a new calibration.
Note: If the calibration data are cleared, the comparison is done with the default values.
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TEMP
pH BUFFERS
°C
°F 1.68 4.01 6.86 7.01 9.18 10.01 12.45
0
32 1.67 4.01 6.98 7.13 9.46 10.32 10.38
5
41 1.67 4.00 6.95 7.10 9.39 10.25 13.18
10
50 1.67 4.00 6.92 7.07 9.33 10.18 12.99
15
59 1.67 4.00 6.90 7.05 9.27 10.12 12.80
20
68 1.68 4.00 6.88 7.03 9.22 10.06 12.62
25
77 1.68 4.01 6.86 7.01 9.18 10.01 12.45
30
86 1.68 4.02 6.85 7.00 9.14
9.96 12.29
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95 1.69 4.03 6.84 6.99 9.11
9.92 12.13
40 104 1.69 4.04 6.84 6.98 9.07
9.88 11.98
45 113 1.70 4.05 6.83 6.98 9.04
9.85 11.83
50 122 1.71 4.06 6.83 6.98 9.01
9.82 11.70
55 131 1.72 4.08 6.84 6.98 8.99
9.79 11.57
60 140 1.72 4.09 6.84 6.98 8.97
9.77 11.44
65 149 1.73 4.11 6.84 6.99 8.95
9.76 11.32
70 158 1.74 4.12 6.85 6.99 8.93
9.75 11.21
75 167 1.76 4.14 6.86 7.00 8.91
9.74 11.10
80 176 1.77 4.16 6.87 7.01 8.89
9.74 11.00
85 185 1.78 4.17 6.87 7.02 8.87
9.74 10.91
90 194 1.79 4.19 6.88 7.03 8.85
9.75 10.82
95 203 1.81 4.20 6.89 7.04 8.83
9.76 10.73
pH BUFFER TEMPERATURE DEPENDENCE
The temperature has an effect on pH. The calibration buffer solutions are affected by temperature
changes to a lesser degree than normal solutions.
During calibration the instrument will automatically calibrate to the pH value corresponding to the
measured temperature.
During calibration the instrument will display the pH buffer value at 25 °C.
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GOOD LABORATORY PRACTICE (GLP)
GLP is a set of functions that allows storage and retrieval of data regarding the maintenance and
status of the electrode.
All data regarding the pH calibration is stored for the user to review when necessary.
EXPIRED CALIBRATION
The instrument is provided with a real time clock (RTC), in order to monitor the time elapsed since
the last pH calibration.
The real time clock is reset every time the instrument is calibrated and the “Expired Calibration”
status is triggered when the instrument detects a calibration time out. The “CAL DUE” tags will start
blinking to warn the user that the instrument should be recalibrated.
The calibration time out can be set (see SETUP for details, page 23) from 1 to 7 days or can be
disabled.
For example, if a 4 days time out has been selected, the instrument will issue the alarm exactly
4 days after the last calibration.
However, if at any moment the expiration value is changed (e.g. to 5 days), then the alarm will be
immediately recalculated and appear 5 days after the last calibration.
Notes: When the instrument is not calibrated or calibration is cleared (default values
loaded) there is no “Expired Calibration”, and the display always shows the “CAL DUE”
tags blinking.
When an abnormal condition in the RTC is detected, the instrument forces the “Expired
Calibration” status.
LAST pH CALIBRATION DATA
The last pH calibration data is stored automatically after a successful calibration.
To view the pH calibration data, press GLP when the instrument is in the measurement mode.
The instrument will display a lot of data including calibration buffer, offset, slope, electrode
condition.
Note: Buffers displayed in video inverse mode are from previous calibrations. The custom
buffers are marked with an “*” on the right side of the buffer value. “No user calibration”
message is displayed if all calibration are cleared or the instrument was not calibrated.
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Backlight
Contrast
Auto light off
Auto power off
Description
Valid value
Backlight Level
Contrast Level
Time until backlight is ON
Time after the instrument is
powered OFF
0 to 7
0 to 20
1, 5, 10, 30 min
Disabled
5, 10, 30, 60 min
Date/Time
01.01.2000 to 12.31.2099
00:00 to 23:59
Time Format
Date Format
AM/PM or 24 hours
DD/MM/YYYY
MM/DD/YYYY
YYYY/MM/DD
YYYY-MM-DD
Mon DD, YYYY
DD-Mon-YYYY
YYYY-Mon-DD
Up to four languages
°C or °F
Enabled or Disabled
0000 to 9999
600, 1200, 2400, 4800,
9600
Language
Temperature Unit
Beep ON
Instrument ID
Baud Rate
Message Display Language
Beeper Status
Instrument Identification
Serial Communication
SETUP
Setup mode allows viewing and modifying the measurement parameters.
The following table lists the general SETUP parameters, their valid range and the factory default
settings.
Default
4
10
1
30
current
date/time
24 hours
YYYY/MM/DD
English
°C
Disabled
0000
9600
Meter Information Displays General Information
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SETUP
The following table lists the specific range parameters.
Item
Description
Calibration Timeout
Number of days after Disable, 1 to 7 days
Calibration warning is
displayed
Management of
Replace or offset
1 point calibration
Custom buffer setting Max. 5 buffers
First point mode
Custom buffer
View calibration
points
Display Out of Cal.
Range Warning
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Display calibration
points
Valid value
Default
Disable
Replace
No
Enable or disabled
Enable
Enable or disabled
Enable
SETUP
GENERAL PARAMETER SCREENS
Backlight
Highlight Backlight.
Press Modify.
Use
/
keys to change the intensity then press Accept to confirm.
Press ESC to leave without changing.
Contrast
Highlight Contrast.
Press Modify.
Use
/
keys to change contrast then press Accept to confirm.
Press ESC to leave without changing.
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SETUP
Auto Light Off
Highlight Auto Light Off.
Press 5, 10 or 30 to change settings.
Auto Power Off
Highlight Auto Power Off.
Press Modify.
Press / keys to select interval then press Accept.
Press ESC to leave without changing.
Date/Time
Highlight Date/Time.
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SETUP
Press Modify.
Use
/
keys to select item.
Use / keys to change focused values.
Press Accept to confirm new setting, or ESC to leave without changing.
Time Format
Highlight Time Format.
Press displayed functional key to change the option.
Date Format
Highlight Date Format.
Press Modify.
Use / keys to select date format then press Accept.
Press ESC to leave without changing.
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SETUP
Language
Highlight Language.
Use the desired functional key to change the option. Wait until new language is loaded. If language
load fails the instrument will try to reload current language.
If any language can’t be loaded, the instrument will work in safe mode. In this mode all messages are
displayed in English and Help is not available.
Temperature Unit
Highlight Temperature Unit.
Press the displayed functional key in order to change the temperature unit.
Beep On
Highlight Beep On.
Press the displayed functional key to enable/disable beep.
When enabled, beep sounds as a short beep every time a key is pressed or when the calibration can
be confirmed.
A long beep alert that the pressed key is not active or a wrong condition is detected while in
calibration.
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SETUP
Instrument ID
Highlight Instrument ID.
Press Modify.
Use / keys to change the instrument ID.
Press Accept to confirm or ESC to exit without saving.
Baud Rate
Highlight Baud Rate.
Press Modify.
Use / keys to select the desired communication baud.
Press Accept to confirm or ESC to exit.
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SETUP
Meter information
Highlight Meter Information.
Press Select.
The meter informations are displayed:
-firmware version
-language version
-mV and temperature factory calibration time/date
-battery capacity
RANGE SPECIFIC PARAMETERS SCREENS
Calibration Timeout
Highlight Calibration Timeout.
Press Modify.
Use / keys to set desired value.
Press Accept to confirm or ESC to return without saving.
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SETUP
Note: If enabled “CAL DUE” warning will be displayed, the set number of days after
calibration is over passed.
First Point Mode
Highlight First Point Mode.
Press the displayed functional key in order to change the option.
If the Replace option is selected, the slopes between current buffer and nearest lower and higher
buffers will be recalculated.
If the Offset option is selected, an electrode offset correction is performed keeping unchanged the
existing slopes.
Custom Buffers
Highlight Custom Buffers.
Press Modify.
Press Delete to delete custom buffer value.
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SETUP
Press Add to add a new buffer to the list (max 5).
Press Modify to set custom buffer value.
Use / keys to change the value.
Press Accept to confirm custom buffer value or ESC to exit without saving.
View Calibration Points
Highlight View Calibration Points.
Press the displayed functional key to change option.
If option is enabled the calibration buffers corresponding to the last calibration are displayed in the
pH measurement screen.
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SETUP
Out of Calibration Range Warning
Highlight Out of Cal.Range Warning.
Press the displayed functional key in order to change option.
If enabled, the “Out Cal Range” message will be displayed if the pH reading is outside. Approxi‑
mately 1 pH unit, from the lowest and highest pH buffer value used for calibration. For single point
pH 7.01 calibration the message will be displayed for pH values less than 4 or greater than 10.
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LOGGING
This feature allows the user to log pH measurements. All logged data can be transferred to a PC
through the USB port using HI92000 application.
The maximum logging space is 200 (100 pH and 100 mV range) for HI98161 record locations.
LOGGING THE CURRENT DATA
To store the current reading into memory, press LOG while in measurement mode.
The instrument will display for few seconds the record number and the amount of the free log
space.
If the LOG space is full, the “Log space is full” message will be displayed for few seconds when
LOG key is invoked. Enter View Logged Data Mode and delete records in order to free log space.
VIEW LOGGED DATA
Press RCL to retrieve the information stored while in measurement mode.
The list of records is displayed.
If no data were logged, the instrument will display “No Records!” message.
Use / keys to scroll between the records from the list.
Press Delete All to enter Delete All screen.
Press Delete to enter Delete records screen.
Press More to view more information of the focused record.
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LOGGING
If More is pressed.
Use / keys to scroll between complete log information.
If Delete is pressed.
Use / key to focus the record to be deleted and then press CFM.
Press ESC to exit.
If Delete All is pressed the instrument asks for confirmation.
Press CFM to confirm or ESC to exit without deleting.
AUTOEND
To freeze the first stable reading on the LCD press AutoEnd while the instrument is in measurement
mode.
The ”Wait” symbol will blink until the reading is stable.
When the reading is stable, “Hold” icon will be displayed.
Press Continue in order to enter continuous reading mode.
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TEMPERATURE CALIBRATION (for technical personnel only)
IMPORTANT NOTE: For factory calibration contact your local Hanna Instruments Office.
All the instruments are factory calibrated for mV and temperature.
Hanna Instruments’ temperature sensors are interchangeable and no temperature calibration is
needed when they are replaced.
If the temperature is inaccurate, calibration should be performed.
For an accurate recalibration, contact your local Hanna Instruments Office or follow the instructions
below.
ENTER CALIBRATION MODE
With the instrument off, press and hold down the
/ then power on the instrument. The
calibration screen is displayed. Highlight “T” and then press Modify to enter in the temperature
calibration mode.
TEMPERATURE CALIBRATION
• Prepare a vessel containing ice and water and another one containing hot water (at approximately
50 °C or 122 °F). Place insulation material around the vessels to minimize temperature changes.
• Use a calibrated thermometer with a resolution of 0.1 °C as a reference thermometer.
• Immerse the pH probe including temperature sensor into the vessel with ice and water as close
as possible to the reference thermometer. Allow a few seconds for the probe to stabilize.
• Use the / keys to set the calibration point value to that of ice and water mixture, measured
by the reference thermometer. When the reading is stable and within range of the selected
calibration point, the CFM functional key is displayed.
36
• Immerse the pH probe including temperature sensor into the second vessel as close as possible
to the reference thermometer. Allow a few seconds for the probe to stabilize.
• Use the / keys to set the calibration point value to that of the hot water.
• When the reading is stable and within range of the selected calibration point, CFM functional key
is displayed.
• Press CFM to confirm. The instrument returns to measurement mode.
TEMPERATURE CALIBRATION (for technical personnel only)
• Press CFM to confirm.
• The second expected calibrated point is displayed.
Note: Use / keys to change calibration point if necessary (±10.0 °C) around the
point. If the reading is not within range of the selected calibration point, “Wrong”
message will blink. Change the pH probe including temperature sensor and restart calibration.
37
PC INTERFACE
Data transmission from the instrument to the PC can be done with the HI92000 Windows®
compatible software (optional). HI92000 also offers graphing and on-line help feature.
Data can be exported to the most popular spreadsheet programs for further analysis.
To connect your instrument to a PC, use an USB cable connector. Make sure that your instrument is
switched off and plug one connector to the instrument USB socket and the other to the serial or USB
port of your PC.
Note: If you are not using Hanna Instruments HI92000 software, please see the following
instructions.
SENDING COMMANDS FROM PC
It is also possible to remotely control the instrument with any terminal program. Use an USB cable
to connect the instrument to a PC, start the terminal program and set the communication options as
follows: 8, N, 1, no flow control.
COMMAND TYPES
To send a command to the instrument follow the next scheme:
<command prefix> <command> <CR>
where: <command prefix> is the 16 ASCII character
<command> is the command code.
Note: Either small or capital letters can be used.
SIMPLE COMMANDS
KF1 Is equivalent to pressing functional key 1
KF2 Is equivalent to pressing functional key 2
KF3 Is equivalent to pressing functional key 3
RNG Is equivalent to pressing RANGE key
MOD Is equivalent to pressing MODE key
CAL Is equivalent to pressing CAL key
UPC Is equivalent to pressing the UP arrow key
DWC Is equivalent to pressing the DOWN arrow key
RCL Is equivalent to pressing RCL key
SET Is equivalent to pressing SETUP key
CLR Is equivalent to pressing CLR key
38
Is equivalent to pressing OFF key
Change the instrument range according with the parameter value (xx):
• xx=00 pH range/0.001 resolution
• xx=01 pH range/0.01 resolution
• xx=02 pH range/0.1 resolution
• xx=03 mV range
The instrument will answer for these commands with:
<STX> <answer> <ETX>
where: <STX> is 02 ASCII code character (start of text)
<ETX> is 03 ASCII code character (end of text)
<answer>:
<ACK> is 06 ASCII code character (recognized command)
<NAK> is 21 ASCII code character (unrecognized command)
<CAN> is 24 ASCII code character (corrupted command)
PC INTERFACE
OFF CHR xx COMMANDS REQUIRING AN ANSWER
The instrument will answer for these commands with:
<STX> <answer> <checksum> <ETX>
where the checksum is the bytes sum of the answer string sent as 2 ASCII characters.
All the answer messages are with ASCII characters.
RAS Causes the instrument to send a complete set of readings in according with the current
range:
• pH, temperature and mV reading on pH range.
The answer string contains:
• Meter mode (2 chars):
• 00 - pH range (0.001 resolution)
• 01 - pH range (0.01 resolution)
• 02 - pH range (0.1 resolution)
• 03 - mV range
• Meter status (2 chars of status byte): represents a 8 bit hexadecimal
encoding.
• 0x10 - temperature probe is connected
• 0x01 - new GLP data available
• 0x02 - new SETUP parameter
• 0x04 - out of calibration range
• 0x08 - the meter is in autoend point mode
39
PC INTERFACE
MDR GLP • Reading status (2 chars): R - in range, O - over range, U - under range. First
character corresponds to the primary reading. Second character corresponds to
mV reading.
• Primary reading (corresponding to the selected range) - 11 ASCII chars, includ‑
ing sign and decimal point and exponent.
• Secondary reading (only when primary reading is not mV) - 7 ASCII chars,
including sign and decimal point.
• Temperature reading - 7 ASCII chars, with sign and two decimal points, always
in °C.
Requests the instrument model name and firmware code (16 ASCII chars).
Requests the calibration data record.
The answer string contains:
• GLP status (1 char): represents a 4 bit hexadecimal encoding.
• 0x01 - pH calibration available
• pH calibration data (if available), which contains:
• the number of calibrated buffers (1 char)
• the offset, with sign and decimal point (7 chars)
• the average of slopes, with sign and decimal point (7 chars)
• the calibration time, yymmddhhmmss (12 chars)
• buffers information (for each buffer)
• type (1 char): 0 - standard, 1 - custom
• status (1 char): N (new) - calibrated in last calibration; O (old) - from
an old calibration.
• warnings during calibration (2 chars): 00 - no warning, 04 - Clean
Electrode warning.
• buffer value, with sign and decimal point and exponent (11 chars).
• calibration time, yymmddhhmmss (12 chars).
• electrode condition, with sign (3 chars). The “-01” code means not calculated.
PAR Requests the setup parameters setting.
The answer string contains:
• Instrument ID (4 chars)
• Calibration Alarm time out for pH (2 chars)
• SETUP information (2 chars): 8 bit hexadecimal encoding.
• 0x01 - beep ON (else OFF)
• 0x04 - degrees Celsius (else degrees Fahrenheit)
• 0x08 - Offset calibration (else Point calibration)
40
PC INTERFACE
• Auto Light Off time (3 chars)
• Auto Power Off time (3 chars)
• The number of custom buffers (1 char)
• The custom buffer values, with sign and decimal point, for each defined custom
buffer (7 chars)
• The short name of the selected language (3 chars)
NSLx Requests the number of logged samples (4 chars).
The command parameter (1 char):
• P - request for pH range
LODPxxx Requests the xxxth pH record logged data.
LODPALL Requests all pH Log on demand.
The answer string for each record contains:
• The logged mode (2 chars):
• 00 - pH range (0.001 resolution)
• 01 - pH range (0.01 resolution)
• 02 - pH range (0.1 resolution)
• 03 - mV range
• Reading status (1 char): R, O, U
• Calculated reading, with sign and decimal point and exponent
(11 chars) - for pH
• Temperature reading, with sign and two decimal points (7 chars)
• mV reading status (1 char): R, O, U
• The mV reading, with sign and decimal point (7 chars)
• The logged time, yymmddhhmmss (12 chars)
• The calibration slope, with sign and decimal point (7 chars)
• The calibration offset, with sign and decimal point (7 chars)
• Temperature probe presence (1 char)
Notes: “Err8” is sent if the instrument is not in measurement mode.
“Err6” is sent if the requested range is not available.
“Err4” is sent if the requested set parameter is not available.
“Err3” is sent if the Log on demand is empty.
“Err9” is sent if the battery power is less than 30%.
Invalid commands will be ignored.
41
BATTERIES REPLACEMENT
To replace the batteries, follow the next steps:
• Turn OFF the instrument.
• Open the battery compartment by removing the four screws from the back of the instrument.
• Remove the old batteries.
• Insert four new 1.5V AA batteries in the battery compartment while paying attention to the correct
polarity.
• Close the battery compartment using the four screws.
If the battery capacity is less than 20 % the serial communication and the backlight feature are not
available.
Note: The instrument is provided with the BEPS (Battery Error Prevention System) feature,
which automatically turns the instrument off when the batteries level is too low to ensure
reliable readings.
42
Since the resistance of the pH electrode is in the range of 50 – 200 MΩ, the current across the
membrane is in the pico Ampere range. Large currents can disturb the calibration of the electrode for
many hours.
For these reasons high humidity environments, short circuits and static discharges are detrimental to
a stable pH reading.
The pH electrode’s life also depends on the temperature. If constantly used at high temperatures, the
electrode life is drastically reduced.
Typical Electrode Life
Ambient Temperature 1 – 3 years
90 °C (194 °F) Less than 4 months
120 °C (248 °F) Less than 1 month
Alkaline Error
High concentrations of sodium ions interfere with readings in alkaline solutions. The pH at which the
interference starts to be significant depends upon the composition of the glass. This interference is
called alkaline error and causes the pH to be underestimated. Hanna Instruments’ glass formulations
have the indicated characteristics.
TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS
The resistance of glass electrodes partially depends on the temperature. The lower the temperature,
the higher the resistance. It takes more time for the reading to stabilize if the resistance is higher. In
addition, the response time will suffer to a greater degree at temperatures below 25 °C (77 °F).
43
ELECTRODE CONDITIONING AND MAINTENANCE
*Not present in gel electrodes.
PREPARATION PROCEDURE
Remove the electrode protective cap.
DO NOT BE ALARMED IF ANY SALT DEPOSITS ARE PRESENT. This is normal with electrodes and they
will disappear when rinsed with water.
During transport tiny bubbles of air may have formed inside the glass bulb. The electrode cannot
function properly under these conditions. These bubbles can be removed by "shaking down" the
electrode as you would do with a glass thermometer.
If the bulb and/or junction are dry, soak the electrode in HI70300 Storage Solution for at least one
hour.
For refillable electrodes:
If the filling solution (electrolyte) is more than 2½ cm (1”) below the fill hole add HI7082 or
HI8082 3.5M KCl Electrolyte Solution for double junction electrodes.
44
MEASUREMENT
Rinse the pH electrode tip with distilled water. Immerse the tip (bottom 4 cm /1½” ensuring the
reference junction is submerged) in the sample and stir gently for a few seconds.
For a faster response and to avoid cross-contamination of the samples, rinse the electrode tip with a
few drops of the solution to be tested, before taking measurements.
STORAGE PROCEDURE
To minimize clogging and assure a quick response time, the glass bulb and the junction of pH
electrode should be kept moist and not allowed to dry out.
Replace the solution in the protective cap with a few drops of HI70300 or HI80300 Storage Solution
or, in its absence, Filling Solution (HI7082 or HI8082 for double junction electrodes). Follow the
Preparation Procedure on page 44 before taking measurements.
Note: NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.
PERIODIC MAINTENANCE
Inspect the electrode and the cable. The cable used for connection to the instrument must be intact
and there must be no points of broken insulation on the cable or cracks on the electrode stem or bulb.
Connectors must be perfectly clean and dry. If any scratches or cracks are present, replace the elec‑
trode.
Rinse off any salt deposits with water.
ELECTRODE CONDITIONING AND MAINTENANCE
For faster response, unscrew the fill hole screw during measurements.
pH Probe Maintenance
For refillable electrodes:
Refill the reference chamber with fresh electrolyte HI7082 or HI8082 for double junction electrodes.
Allow the electrode to stand upright for 1 hour.
Follow the Storage Procedure above.
pH CLEANING PROCEDURE
•General Soak in Hanna Instruments HI7061 or HI8061 General Cleaning Solution for approxi‑
mately ½ hour.
• Protein Soak in Hanna Instruments HI7073 or HI8073 Protein Cleaning Solution for 15 minutes.
• Inorganic Soak in Hanna Instruments HI7074 Inorganic Cleaning Solution for 15 minutes.
• Oil/grease Rinse with Hanna Instruments HI7077 or HI8077 Oil and Fat Cleaning Solution.
45
ELECTRODE CONDITIONING AND MAINTENANCE
46
Instruction for Application Cleaning Solution
1. Add in a beaker about 50 - 75 mL (or minimum to cover the electrode junction) one of the
following cleaning solution:
• Cleaning and Disinfection Solution for Dairy Products (HI70641);
• Cleaning and Disinfection Solution for Yogurt Products (HI70643);
• Acid Cleaning Solution for Meat, Grease and Fats (HI70630);
• Cleaning Solution for Cheese Deposits (HI70642);
• Alkaline Cleaning Solution for Meat, Grease and Fats (HI70631);
• Cleaning Solution for Milk Deposits (HI70640).
2. Soak the electrode for several minutes (5 - 15) while moderately stirring the solution.
3. Remove the electrode from the cleaning solution and rinse it thoroughly with deionized water to
remove all traces of the cleaning solution.
4. After cleaning and rinsing it is preferable to store it in an electrolyte solution for about 1 hour.
5. Rinse the electrode thoroughly with deionized water and measure samples as usual.
Note: If the electrode response is slow or the electrode does not calibrate correctly, repeat the
cleaning procedure.
SYMPTOMS
Slow response/excessive drift.
PROBLEM
Dirty pH electrode.
SOLUTION
Soak the electrode tip in HI7061
solution for 30 minutes and then
follow the Cleaning Procedure.
Reading fluctuates up and down (noise). Clogged/dirty junction. Low
electrolyte level (refillable
electrodes only).
Clean the electrode. Refill with fresh
electrolyte (refillable electrodes
only).
Display shows blinking full scale value.
Reading out of range.
Check that sample is within measur‑
able range
Display shows "Clean electrode"
blinking.
Difference between new and
previous calibration has been
detected.
Clean electrode and recalibrate.
If the problem remains, check the
buffer solutions.
Meter fails to calibrate or gives faulty
readings.
Broken pH electrode.
Replace electrode.
Error messages are displayed during pH
calibration procedure.
Wrong or contaminated buffer,
electrode dirty or broken.
Check that buffer solution is correct
and fresh.
Meter shuts off.
Dead batteries; Auto-off feature
is enabled: in this case, meter
shuts off after selected period of
non-use.
Replace the batteries; Press ON/OFF.
"Errxx" message at start up.
Internal error.
Contact your local Hanna
Instruments Office.
The instrument does not start when
pressing ON/OFF.
Initialization error.
Press and hold down ON/OFF for
about 20 seconds or disconnect and
then connect the batteries.
TROUBLESHOOTING GUIDE
IMPORTANT: After performing any of the cleaning procedures, rinse the electrode thoroughly
with distilled water, refill the reference chamber with fresh electrolyte (not necessary for gel-filled
electrodes) and soak the electrode in HI70300 or HI80300 Storage Solution for at least 1 hour
before taking measurements.
47
ACCESSORIES
Electrode Cleaning Solution
Code
Description
HI70000P
HI700601P
Electrode Rinse Solution, 20 mL sachet, 25 pcs.
General purpose cleaning solution for laboratories, 20 mL sachet, 25 pcs.
HI700630P
HI7061L
HI7073L
HI7074L
Acid cleaning solution for meat grease and fats (food industry),
20 mL sachet, 25 pcs.
Cleaning solution for milk deposits (food industry), 20 mL sachet, 25 pcs.
Cleaning and disinfection solution for dairy products (food industry),
20 mL sachet, 25 pcs.
Cleaning solution for cheese residues (food industry), 20 mL sachet, 25 pcs.
Cleaning and disinfection solution for yogurt products (food industry)
20 mL sachet, 25 pcs.
General Purpose Cleaning Solution, 500 mL bottle
Protein Cleaning Solution, 500 mL bottle
Inorganic Cleaning Solution, 500 mL bottle
HI7077L
Oil and Fat Cleaning Solution, 500 mL bottle
HI8061L
General Purpose Cleaning Solution, FDA approved bottle, 500 mL
HI8073L
Protein Cleaning Solution, FDA approved bottle, 500 mL
HI8077L
Oil and Fat Cleaning Solution, FDA approved bottle, 500 mL
HI70630L
Acid cleaning solution for meat grease and fats (food industry),
500 mL bottle
Alkaline cleaning solution for meat grease and fats (food industry),
500 mL bottle
Cleaning solution for milk deposits (food industry), 500 mL bottle
HI700640P
HI700641P
HI700642P
HI700643P
HI70631L
HI70640L
HI70641L
HI70642L
HI70643L
48
Cleaning and disinfection solution for dairy products (food industry),
500 mL bottle
Cleaning solution for cheese residues (food industry), 500 mL bottle
Cleaning and disinfection solution for yogurt products (food industry),
500 mL bottle
HI50004-01
pH 4.01 Buffer Solution, 20 mL sachet, 10 pcs.
HI50004-02
pH 4.01 Buffer Solution, 20 mL sachet, 25 pcs.
HI50007-01
HI50007-02
pH 7.01 Buffer Solution, 20 mL sachet, 10 pcs.
pH 7.01 Buffer Solution, 20 mL sachet, 25 pcs.
HI50010-01
pH 10.01 Buffer Solution, 20 mL sachet, 10 pcs.
HI50010-02
pH 10.01 Buffer Solution, 20 mL sachet, 25 pcs.
HI5016
HI5004
pH 1.68 Buffer Solution, 500 mL bottle
pH 4.01 Buffer Solution, 500 mL bottle
HI5068
pH 6.86 Buffer Solution, 500 mL bottle
HI5007
pH 7.01 Buffer Solution, 500 mL bottle
HI5091
HI5010
HI5124
HI8004L
HI8006L
HI8007L
pH 9.18 Buffer Solution, 500 mL bottle
pH 10.01 Buffer Solution, 500 mL bottle
pH 12.45 Buffer Solution, 500 mL bottle
pH 4.01 Buffer Solution in FDA approved bottle, 500 mL
pH 6.86 Buffer Solution in FDA approved bottle, 500 mL
pH 7.01 Buffer Solution in FDA approved bottle, 500 mL
HI8009L
HI8010L
pH 9.18 Buffer Solution in FDA approved bottle, 500 mL
pH 10.01 Buffer Solution in FDA approved bottle, 500 mL
ACCESSORIES
pH Calibration Solution
Code
Description
Electrode Storage Solution
Code
Description
HI70300L
Storage Solution, 500 mL bottle
HI80300L
Storage Solution in FDA approved bottle, 500 mL
49
ACCESSORIES
Other Accessories
Code
FC2023
HI92000
HI920015
HI740157P
HI720161
Description
PVDF body, pH probe with internal temperature sensor
PC Software
USB Cable
Electrode refilling pipette
Hard carrying case
MAN98161 08/17
50
Warranty
The HI98161 is warranted for two years against defects in workmanship and materials
when used for their intended purpose and maintained according to instructions.
Electrodes and probes are warranted for six months. This warranty is limited to repair
or replacement free of charge.
Damage due to accidents, misuse, tampering or lack of prescribed maintenance is
not covered.
If service is required, contact your local Hanna Instruments Office. If under warranty,
report the model number, date of purchase, serial number and the nature of the
problem. If the repair is not covered by the warranty, you will be notified of the
charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain
a Returned Goods Authorization (RGA) number from the Technical Service department
and then send it with shipping costs prepaid. When shipping any instrument, make
sure it is properly packed for complete protection.
Hanna Instruments reserves the right to modify the design, construction or appearance of its products
without advance notice.
World
Headquarters
Hanna Instruments Inc.
Highland Industrial Park
584 Park East Drive
Woonsocket, RI 02895 USA
www.hannainst.com
Local Office
Hanna Instruments USA
270 George Washington Highway
Smithfield, RI 02917
Phone: 800.426.6287
Fax: 401.765.7575
e-mail: tech@hannainst.com
MAN98161
Printed in ROMANIA
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