embedding - Science Services

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SerVices
Embedding
Molds and Materials
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Content
Embedding Media Kits.........................................................................................................3
Technical Tips: the Storing of Embedding Resin Mixtures.........................................................................................4
Technical Tips: Improved Polymerization of Acrylic Resins.......................................................................................4
Acrylic Resins............................................................................................................................ 4
Technovit® 7100 and 8100 Embedding Kits..............................................................................................................4
Technovit® 3040 – Mounting Medium....................................................................................................................... 5
Technovit® 9100 ....................................................................................................................................................... 5
LR White Resin ......................................................................................................................................................... 6
LR Gold Resin............................................................................................................................................................ 7
Acrylic Plastic Casting................................................................................................................................................ 7
JB-4™ Embedding Kit ............................................................................................................................................... 7
JB-4 Plus™ Embedding Kit........................................................................................................................................ 8
Histocryl Resin .......................................................................................................................................................... 8
Lowicryl Resin............................................................................................................................................................ 9
Lowicryl MonoStep Single Component Embedding Media........................................................................................9
Methyl Methacrylate/Butyl Methacrylate ................................................................................................................... 9
Unicryl ™................................................................................................................................................................... 9
Micro-Bed Resin ..................................................................................................................................................... 10
Immuno-Bed ™ Embedding Resin.......................................................................................................................... 10
GMA, Water Soluble................................................................................................................................................. 11
GMA/PEG................................................................................................................................................................ 11
HPMA, Water Soluble.............................................................................................................................................. 11
Epoxy Resins........................................................................................................................... 11
Technical Tips: General Tips for Embedding Media Kits..........................................................................................11
Epo-Fix .................................................................................................................................................................... 12
Quetol 651 – NSA.................................................................................................................................................... 13
EMbed-812.............................................................................................................................................................. 13
EMbed-812/ DER 73................................................................................................................................................ 14
UltraBed Kit.............................................................................................................................................................. 14
Araldite 502.............................................................................................................................................................. 14
Araldite 6005............................................................................................................................................................ 15
Araldite, Embed 812 (Epon-812)............................................................................................................................. 15
Durcupan, Water Soluble......................................................................................................................................... 15
Durcupan ACM, Epoxy Resin.................................................................................................................................. 15
Low Viscosity, as reported by Dr. Spurr................................................................................................................... 15
DER 332-732........................................................................................................................................................... 16
Polyurethane Media................................................................................................................. 16
Crystal Clear™ - Water Liquid Plastics.................................................................................................................... 16
Freezing Media......................................................................................................................... 17
Tissue-Tek® O.C.T. Compound .............................................................................................................................. 17
Tissue Freezing Medium; TFM™............................................................................................................................. 17
Cryogenic-Gel.......................................................................................................................................................... 17
Paraffin & Wax.......................................................................................................................... 18
Polyester Wax.......................................................................................................................................................... 18
Paramat and Paramat Extra ................................................................................................................................... 18
Tissue-Tek® V.I.P.™................................................................................................................................................ 19
Carbowax Polyethylene Glycol................................................................................................................................ 19
Peel Away Paraffin Embedding Wax........................................................................................................................ 20
Polyfin™................................................................................................................................................................... 20
Paraplast Embedding Medium................................................................................................................................. 20
Polyester.................................................................................................................................. 21
Styrene Monomer, Polyester Resin......................................................................................................................... 21
Technovit 4000......................................................................................................................................................... 21
Vestopal ® 310........................................................................................................................................................ 21
Other......................................................................................................................................... 22
Diethylene Glycol Distearate (DEGDS/DGD)..........................................................................................................22
Gach (Glutaraldehyde/Carbohydrazide) ................................................................................................................. 22
Hard-Plus Resin-812................................................................................................................................................ 22
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Embedding Capsules and Holders..................................................................................23
Capsules; Embedding, Size 00, 8mm inner diameter..............................................................................................23
Embedding Capsule; Flat......................................................................................................................................... 23
Embedding Capsule Holder..................................................................................................................................... 23
EMS and BEEM® Embedding Capsules Size 3 (small)..........................................................................................23
BEEM® Embedding Capsules; Special Shapes......................................................................................................24
BEEM® Capsule Holders........................................................................................................................................ 24
The Opaque BEEM® Capsule Holder..................................................................................................................... 24
BEEM® Block Storage............................................................................................................................................. 25
Capsule Block Storage ........................................................................................................................................... 25
Capsules; Gelatin..................................................................................................................................................... 25
Large Gelatin Capsules........................................................................................................................................... 25
Gelatin Capsule Holder............................................................................................................................................ 26
BEEM® Specimen Block Storage System 2000®...................................................................................................26
Plastic Flat Embedding Mold................................................................................................................................... 28
BEEM®™ Capsule Press........................................................................................................................................ 28
Easy-Molds™........................................................................................................................................................... 29
Embedding Molds..............................................................................................................30
Flat Embedding Mold............................................................................................................................................... 30
Small Embedding Mold............................................................................................................................................ 30
Round Embedding Mold.......................................................................................................................................... 30
Cochlea Embedding Mold........................................................................................................................................ 30
Double End Flat Mold.............................................................................................................................................. 31
Large Flat Embedding Mold with Trapezoids...........................................................................................................31
Large Flat Embedding Mold with rectangles............................................................................................................31
Capsule Molds......................................................................................................................................................... 32
The Embedding Capsule Substitute:....................................................................................................................... 32
The Mold for Long Tissues:...................................................................................................................................... 32
The Cell Culture Mold:............................................................................................................................................. 33
Chien Silicone Universal-Mold................................................................................................................................. 33
Small Flat Embedding Mold..................................................................................................................................... 33
Double Tapered Ends.............................................................................................................................................. 34
Triple Tapered Ends................................................................................................................................................. 34
Flat Embedding Molds............................................................................................................................................. 34
Stepped Microtome Embedding Mold...................................................................................................................... 34
Slide Duplicating Molds............................................................................................................................................ 35
Slide Duplicating With Cover Slip Mold.................................................................................................................... 35
Chang Monolayer Molds.......................................................................................................................................... 35
Flat Embedding For Acrylics.................................................................................................................................... 36
BEEM® Flat Embedding Molds............................................................................................................................... 36
Tissue Culture Monolayer Embedding Mold: Thompson Embedding Mold.............................................................36
Blue Silicone Rubber............................................................................................................................................... 37
Clear Silicone Rubber.............................................................................................................................................. 38
Disposable Flat Embedding Mold............................................................................................................................ 38
Tissue-Tek® Cryomold®; Sakura Finetek................................................................................................................39
Disposable Base Mold............................................................................................................................................. 39
Peel Away Disposable Embedding Molds ...............................................................................................................39
Cocoon Box – A Smart Box...................................................................................................................................... 40
Embedding Supplies..........................................................................................................42
Liquid Blocker–Super PAP Pen............................................................................................................................... 42
Fro-Marker............................................................................................................................................................... 42
Para-Marker............................................................................................................................................................. 42
Butler Block Trimer................................................................................................................................................... 42
Aluminum Chucks.................................................................................................................................................... 43
Ultramicrotomy Chucks............................................................................................................................................ 43
EBH-2 Block Holder................................................................................................................................................. 43
Tissue-Tek® Mold Release, Concentrate................................................................................................................ 44
Liquid Release Agent............................................................................................................................................... 44
TFE-like Spray / Mold Release Spray ..................................................................................................................... 44
Para/Gard™............................................................................................................................................................. 44
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Embedding Devices...........................................................................................................45
Tissue Embedding Center ...................................................................................................................................... 45
THE EMS Tissue Embedding Center....................................................................................................................... 46
Paraffin Section Mounting Bath............................................................................................................................... 47
Paraffin Wax Dispenser Electrothermal .................................................................................................................. 48
The following icons are used in this catalogue:
RT
Chemicals best stored at room temperature
0-5°C
Chemicals best stored between 0°C and 5°C
TECH
Technical data sheets available at www.scienceserices.de
The following trademarks are used in this catalogue:
BEEM® is a registered trademark of Better Equipment For Electron Microscopy, Inc.
EXART is a registered trademark of EXART-Apparratebau GmbH & Co. KG, D-22851 Norderstedt
Technovit® is a registered trademark for Heraeus Kulzer, Germany,
Durcupan™ is a registered trademark of A. Fluka A.G. Switzerland
Tissue Tek.® is a registered trademark of Sakura Finetek, USA
Cryo-Gel™ is a registered trademark of Aspen Aerogels, Inc.
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Embedding Media Kits
SCIENCE SERVICES
Embedding Media Kits
Technical Tips: the Storing of Embedding Resin Mixtures
Our experience over the years shows that the best way to store remaining embedding resin mixtures is in
disposable plastic syringes.
Procedure:
Take a fresh “all plastic” syringe (e.g.. #72520-72529- choose the appropriate size syringe depending on the
volume mixture to be stored), remove the tip cover, slowly draw in the mixture. Remove any air space by
holding the syringe with the tip in the upward position pulling the plunger slowly to clear the resin from the tip
as well as letting the air move towards the tip; slowly push the plunger upward until the resin appears at the
end of the tip; put on the tip cover. Wrap the syringe with aluminum foil, label it (name, mixture, and date)
and store it in the refrigerator. This method protects the mixture from contamination by condensation, and it
is also more convenient for future use.
Technical Tips: Improved Polymerization of Acrylic Resins
Acrylic resins (Lowicryl, LR White, LR Gold, JB4) have become common embedding media for
immunocytochemistry. A common problem in handling and disposing of these media is that oxygen inhibits
their polymerization. Bubbling dry nitrogen gas through the resin mixture before infiltrating and embedding
drives off oxygen and insures consistent polymerization. Specimens embedded in gelatin capsules can be
placed in a small chamber or vacuum oven which can be purged with nitrogen to insure complete
polymerization of blocks and avoid the tacky block surface and noxious, volatile vapors from incompletely
cured blocks.
Reference:
E. Ann Ellis, Ellis EM Consulting Service, 1316 SW 61st Terrace, Gainesville, FL 32607.
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Embedding Media Kits
Acrylic Resins
Technovit® 7100 and 8100 Embedding Kits
RT
Technovit® Glycol Methacrylate embedding kits
developed by Kulzer in Germany are the most
convenient to use, and offers the best embedding
results.
Features:
•
•
•
•
•
Technovit® GMA kits give better results offering improved morphology, and allows for 1 micron serial
sectioning.
Technovit® 7100 GMA is less sensitive to O2 during polymerization so sealing of the molds is not
necessary.
The polymerization temperature never exceeds 40°C, which makes either kit a potential choice for
enzyme histochemistry. Initiation chemistry of Technovit® 8100 GMA makes polymerization at 4°C
possible for improved signal during immunohistochemistry.
The initiator is prepackaged, eliminating messy handling of dry components, and by eliminating
aromatic amines from the formation, the components are significantly less toxic.
The consistent high quality of raw materials used to make the resin allows for a permanent clear
colorless block and, most important, reproducible results.
Product Code
14653
Technovit® H7100 –GAM kit consists of:
Glycol Methacrylate monomer
pack Hardener I
Hardener II
Content
500ml
5x0,6g
40ml
Product Code
14654
Technovit® H8100 –GAM kit consists of:
Glycol Methacrylate monomer
pack Hardener I
Hardener II
Content
500ml
5x0,6g
30ml
Technovit® 3040 – Mounting Medium
RT
Technovit 3040 yellow is a fast curing methyl methacrylate-based resin, whose chemical composition
warrants a firm, durable bond between Technovit and the specimen.
Technovit® 3040 consists of two components – powder and liquid – allowing simple mixing, easy adherance
to the specimen, and fast curing. For fixing the mount, a high viscous consistency is required (i.e. a mixing
ratio of approximately 2-3 parts per volume powder: 1 part per volume liquid) has proven to be the most
advantageous.
Product Code
14652
5
Technovit® 3040 Kit consists of:
Technovit® 3040 powder
Technovit® 3040 liquid
Content
1x100g
1x80ml
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Embedding Media Kits
Technovit® 9100
RT
Technovit® 9100 is an embedding medium system based on methyl methacrylate (MMA), which hardens at
low temperature. It is designed for the embedding of mineralized tissues with extensive possibilities of
staining for light microscopy.
Product Code
14655
Kit consists of
1000ml Basic Solution (stabilized)
120g PMMA-Powder
8 Bags @ 1g Hardener 1
10ml Hardener 2
5ml Polymerization Regulator
500gPMMA-Granulate, EXART
Product Code
14655-1
14655-2
14655-3
14655-4
Description
Technovit 9100 Hardener 1
Technovit 9100 Hardener 2
Technovit 9100 Liquid
Technovit 9100 Powder
Applications:
•
Heavy duty microtome technique for preparing thin sections – such as pelvic biopsy, small low and
high density bone samples.
•
Cutting grinding and micro-grinding system (contact point technique) - such as jaw and teeth
segments with and without implant.
•
Combined contact point and heavy duty microtome technique (target preparation) - such as interface
regions and examination of tissues surrounding metal implants and cement-free endoprothesis
(target tissue preparation).
Properties:
Technovit® 9100 Polymerization takes place under the exclusion of oxygen with the help of a peroxide/amine
catalyst. Additional components such as PMMA-powder and a regulator allows for a controlled
polymerization at temperatures between –8 and –20°C, which guarantees for the dispersion of the heat
generated during polymerization. The polymerization time is between 18 and 24 hours using a volume
between 3 and 15 ml in the above temperature range. All the routine techniques such as preparation tissues,
staining, immuno- and enzyme-histochemistry, in-situ hybridization techniques can be performed.
•
•
•
•
•
•
Basic Solution – is composed of organic monomers, with at least one carbon-carbon double bond
(C=C). The stabilizer added to this solution is for storage-stability. Hydrophilic properties are
improved by the addition of a special hydrophilic generating agent.
PMMA-Powder – is an internal filler and it is made up of PMMAmicro pellets. It is used to:
-Reduce the polymerization shrinking effect.
-Reduce the heat generated during the polymerization process.
-Improve the quality of the polymerization block.
Hardener 1 – is one of the components of the polymerization initiation system. It is a derivative of
dibenzoyl peroxide, which in combination with Hardener 2, makes the polymerization take place.
Hardener 2 – it works catalytically upon hardener 1 to allows for a controlled polymerization, even at
temperatures below 0°C.
Regulator – is composed of a reactive organic compound, which allows a controlled polymerization,
even with large volumes of polymer, without increases in the temperature during the polymerization
reaction.
PMMA-Granulate, EXART® - this granulate acts as an additional internal filler when larger amounts
(500-1000ml) of polymer are to be used, for example, in the case of femur shaft with noncemented
endoprosthses. The amount of monomer (basic solution) is thereby reduced, at the same time
making the polymerization easier to control.
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LR White Resin
Embedding Media Kits
0-5°C
A very low viscosity (8cps), non-toxic resin, suitable for both Light and Electron Microscopy.
LR White is a polar monomer polyhydroxylated acromatic acrylic resin. It
can be cured by heat or by UV light. Sections of polymerized LR White resin
are hydrophilic. This character allows immuno-cytochemistry reagents to
easily penetrate into the sections and etching of the sections is not
necessary (etching sections will effect delicate tissue antigens). LR White
sections also show minimal non-specific staining. Its low viscosity makes it
ideal for infiltrating decalcified bone and teeth as well as plant tissues. It is
available in medium and hard grade. We recommend storage at 4°C.
Newman, G.R. (1987). Use and abuse of LR White. Histochem. J., 19:118
Newman, G.R. (1989). LR White embedding medium for colloidal gold
methods. In: Colloidal Gold: Principles, Methods, and Applications, Vol.2
(Hayat, M.A., Ed.). Academic Press. San Diego and London
Product Code
14380
14381
14382
14383
14385
LR Gold Resin
Description
LR White Medium Grade Kit, uncatalyzed
LR White Resin only, Medium, uncatalyzed
LR White Hard Grade Kit, uncatalyzed
LR White Resin only, Hard, uncatalyzed
LR White Accelerator
Content
Kit
Kit
Kit
Kit
10ml Accelerator
RT
The London Resin Company's acrylic "LR Gold" offers the histochemist and immunocytochemist the
advantage of working with unfixed tissues at low temperatures, thus allowing many fixation-sensitive
procedures to be carried out in 1-4 microns resin sections at -25°C using visible light. It is non-toxic and has
a low viscosity.
Product Code
14370
Kit consists of
500ml LR Gold Resin
100g Polyvinylpyrolidone
50g Benzoyl Peroxide
50g Benzil
Product Code
14370
14371
Description
LR Gold Kit
LR Gold Resin only
Content
Kit
500ml
Acrylic Plastic Casting
Simply place your object in a mold, mix and pour Acrylic Plastic over it. In an hour it is hard and your object is
firmly embedded in a clear, stable plastic. For high-gloss finish, it can be polished with a soft cloth.
Mold making and casting can be done in two hours. Acrylic Plastic Casting is a thermosetting acrylic-polymer
plastic with excellent clarity and stabile characteristics.
Product Code
24210-01
24210-02
24210-08
7
Description
Acrylic Plastic Casting
Acrylic Plastic Casting
Acrylic Plastic Casting
Pack
1 pt Kit
1 qt Kit
1 gal Kit
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Embedding Media Kits
JB-4™ Embedding Kit
RT
A water soluble embedding media which is based on Glycol Methacrylate (GMA) plastic embedding. It is
intended for use in preparing samples for high resolution microscopes (HRLM). The catalyzed monomer acts
as a dehydration and infiltration agent; therefore, complete dehydration through 100% ethanol is not
necessary (although recommended for large or dense tissues). Conventional paraffin sections have a greater
degree of shrinkage and produce inadequate morphology when compared to JB-4.
Features:
•
Thin sections (.5-2.0 µm) with excellent morphological structure preservation.
•
Water-clear blocks; casts in 90 minutes max. at room temp.
•
Good lipid and enzyme retention when processing at low temperatures (4°C).
•
Removal of JB-4 resin prior to staining is not necessary.
•
Clearing agents such as xylene and chloroform are not needed.
•
Higher clarity and contrast than with paraffin sections.
•
Easy processing of difficult specimens such as calcified bone and delicate embryonic tissue.
References:
•
Hofman E.O. & Flores, T.R., High Resolution LM in Renal Path., Amer. J. of Clin. Path., 76:5 (1981)
•
Beckstead, J.H., Blood, 57(6):1008 (1981)
•
Brinn, N & Pickett, P., J. Histotech., 2(5):125-130 (1979)
•
Block, Matthew H., et al., Lab. Med., 13(5)(1982)
•
Helander, K.G., J. Microscopy, 132:223 (1983)
•
Cole, M.B., J. Microscopy, 127:139 (1982)
•
Higuchi, S., et al., Stain Tech., 54(1):5-12 (1979)
•
Van DeVeldt, S., Am. J. Clin. Path.,73:121 (1980)
•
Horton, W.A., J. Histochem. Cytochem., 31:417 (1983)
•
Tacha, D.E. & Richard, T.C.., J. Histotech., 4(2):59 (1981)
Product Code
14270-00
Kit consists of
800ml Solution A
30ml Solution B
12g Catalyst Kit
JB-4 Plus™ Embedding Kit
Product Code
14270-01
14270-04
14270-06
Description
JB-4 Solution A only
JB-4 Solution B only
JB-4 Catalyst only
Content
800ml
30ml
12g
RT
JB-4 Plus offers all of the same features as JB-4 with the following additions:
•
•
It produces less of an exothermic reaction than JB4 which is good for temperature sensitive tissues.
It produces harder blocks which is ideal for dense samples, such as bone
Product Code
14270-00
Kit consists of
500ml Solution A
15ml Solution B
8g Catalyst Kit
Product Code
14272-01
14272-04
14272-06
Description
JB-4 Plus Solution A only
JB-4 Plus Solution B only
JB-4 Plus Catalyst only
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Content
500ml
15ml
8g
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Histocryl Resin
Embedding Media Kits
RT
Histocryl acrylic resin has been specially formulated for light microscopy. It is water clear, and because it is
hydrophilic (polar) it permits the use of most routine staining techniques without prior removal or etching.
Product Code
14370
Kit consists of
500ml Histocryl Resin
50g Histocryl Catalyst (B.P.)
10ml Histocryl Accelerator
Lowicryl Resin
RT
TECH
Lowicryl resins are highly cross-linked acrylate-based embedding resins, designed for use over a wide range
of embedding conditions. These resins provide low viscosity at low temperatures.
The K4M kit is usable to -35°C and is a polar (hydrophilic) medium, while the HM20 kit can be used down to
-70°C and is nonpolar (hydrophobic).
Lowicryl's K11M and HM23 have properties similar to K4M and HM20, but can be used at temperatures 20
degrees lower than others: K11M at -60°C, HM23 at least -80°C. All of these resins are photopolymerized by
long wavelength (360nm) ultraviolet light. Since the initiation of the polymerization is largely independent of
temperatures, blocks may be polymerized at the same temperatures used for infiltration. The resin may also
be chemically polymerized at 60°C.
Product Code
14330
14340
14350
14360
Description
K4M Kit
HM20 Kit
K11M Kit
HM23 Kit
Content
Kit
Kit
Kit
Kit
Lowicryl MonoStep Single Component Embedding Media
RT
The same as our Lowicryl complete kits but this new formulation allows you one stop embedding without all
of the cumbersome preparation and mixing. This pre-mixed, ready to use resin, saves you time and
minimizes chemical contact. MonoStep Lowicryl is ideal for use in immunohistochemistry and
immunolabeling. Store at room temperature.
Product Code
14335
14345
Description
Lowicryl HM-20 Polar
Embedding Medium; MonoStep
Lowicryl HM-20 Polar
Embedding Medium; MonoStep
Methyl Methacrylate/Butyl Methacrylate
Content
225g
225mg
RT
The mixture of Butyl and Methyl Methacrylate offers many advantages over the standard GMA and HPMA
embedding media:
Product Code
14520
9
Description
Methyl Methacrylate
Butyl Methacrylate
Benzoyl Peroxide Paste
Content
225ml
225ml
15g
•
•
It can be UV cured in the cold with the
addition of benzoin methyl ether, or heat
cured by the addition of benzoyl peroxide.
The resin can be removed from the
sections with a solvent (acetone) before
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Embedding Media Kits
staining.
Unicryl ™
0-5°C
A New Universal Resin for:
•
•
•
•
•
Light Microscopy
Electron Microscopy
Immunolabeling
In-Situ Hybridization
Histochemistry
UNICRYL is a single component and easy to use resin which gives excellent structural preservation of
tissues without chemically interacting or crosslinking with them. UNICRYL is largely hydrophilic, allowing
good access to polar solutions and exhibiting low background staining or labeling from hydrophobic
materials. It also minimizes the denaturation of proteins, allowing true antigenic properties to be maintained.
The excellent polymerization properties of UNICRYL are largely due to the fact that all of the components of
the polymer have similar molecular weights, ensuring even penetration into the tissue.
In addition, the enhanced labeling, staining and hybridizing qualities arise from the fact that sections are
cleaved from the block face ahead of the knife edge, thus exposing more of the tissue components at the
surface. UNICRYL is premixed and supplied as a single component. The resin has a long shelf life when
stored in the cold. It is miscible with alcohol and has a low viscosity even down to -50°C. The resin can be
polymerized by heat (50-60°C) or by UV
irradiation at low temperatures (-10 to 20°C).
Product Code
Kit consists of
The polymerization of the resin is an exothermic
14660
250 ml Unicryl Resin
reaction. Unicryl shrinks approximately 10% in
1 pack of 1ml embedding vials
volume during polymerization. Ethyl alcohol,
1 pair plastic gloves
acetone and many other solvents may be used
1 plastic bag for disposal
as a dehydrating agent.
1 waste container
Unicryl Embedding Kit
Complete instructions come with the kit.
Micro-Bed Resin
0-5°C
Micro-Bed resin is a newly developed water-soluble resin which is a mixture of acrylate and polyester resins.
It is a very universal embedding medium and may be used for either light or electron microscopy.
Due to its non-crosslinking characteristics, its hydrophilic nature, and roughness of its section surface, our
Micro-Bed Resin is excellent in preserving tissue structure, offers good results in immunolabeling, and
stands up well to conventional stains.
In light microscopy, tissues were stained with conventional histological stains (e.g.,hematoxylin-eosin) and
embedded in Micro-Bed: the results were comparable with those provided on paraffin embedded tissues.
Further, polychrome silver-based and cytochemical stains (e.g.,PAS) were also feasible. In transmission
electron microscopy, images as good as those obtained with similar acrylic resins, (e.g., Lowicryl K4M), were
obtained.
Micro-Bed is supplied as a single component-ready to use pre-mixed resin. Micro-Bed can be polymerized in
either EMS embedding capsules or gelatin capsules by either heat(55-65°C) or UV (wavelength 365nm) light
at low temperatures (-10 to 20°C) for 72 hours.
Scala C. et al.(1992). Development of a new acrylic resin ideally suited for light and electron microscopy.
Electron Microscopy. Volume 3.EUREM 92, Granada, Spain. pg. 271
Product Code
14210
Description
Micro-Bed Embedding Resin
Content
500ml
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Immuno-Bed ™ Embedding Resin
•
•
0-5°C
Embedding Media Kits
TECH
A low viscosity media for light microscopy and immuno-histochemistry techniques.
It allows for the penetration of large immunoglobulins through the plastic sections for demonstration
of antigenic sites.
J. Histochem, Cytochem., 35:595 (1987)
Product Code
14260-00
Kit consists of
750ml Solution A
50ml Solution B
9g Catalyst Kit
GMA, Water Soluble
Product Code
14260-01
14260-04
14260-06
Description
Immuno-Bed Solution A only
Immuno-Bed Solution B only
Immuno-Bed Catalyst only
Content
800ml
30ml
12g
RT
Glycol Methacrylate embedding medium provides excellent preservation (especially enzymatic
autoradiographic studies). Good for E.M., L.M., and Histo. Leduc. & Bernard, (1967).
J. Ultrastr. Res., 4, 196-99.
Product Code
14200
GMA/PEG
Kit consists of
450ml GMA
225ml n-Butylmethacrylate
15g 2,4-Dichlorobenzoyl Peroxide
paste
RT
Polyethylene Glycol - Glycol methacrylate mixture. A water miscible embedding medium for cytochemical
studies and enzyme localization when light and electron microscopy need to be correlated.
Spaur, R.C. & Moriarty, G., (1977) J Histochem. Cytochem., 23:16
Product Code
14250
Kit consists of
450ml Glycol methacrylate, GMA
225ml n-Butyl methacrylate
50ml Ethylene glycol dimethacrylate
15g Benzoyl Peroxide Paste
20ml Polyethylene Glycol 400
HPMA, Water Soluble
RT
TECH
HPMA is a water-soluble resin used for cytochemical applications. Infiltration follows fixation of the tissue so
there is no extraction of materials caused by dehydration in alcohols. Leduc, E.H. et al., (1965) J. Cell Biol.,
26:137.
Product Code
14220
11
Kit consists of
450ml HPMA
15g Benzoyl Peroxide Paste
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Embedding Media Kits
SCIENCE SERVICES
Epoxy Resins
Technical Tips: General Tips for Embedding Media Kits
Epoxy Resins: Problems, Causes, and Solutions
Problems associated with poor embedding in epoxy resins:
•
Blocks are difficult to section
•
Sections may disintegrate upon contact with water or the electron beam
•
Holes in the sections
•
Unevenly cured blocks
•
Blocks are either too soft or too hard
Factors that cause poor embedding in epoxy resins:
•
The presence of water in absolute dehydration solvents and in the ingredients of the embedding
mixture
Solutions to the causes:
•
Use either fresh or well capped bottles of dehydration solvents.
•
Do not open cold bottles. Water may condense inside the bottle.
•
Embedding should be carried out at relative humidities below 50%. Dellman, H.D. and Pearson, C.B.
(1977).“Better epoxy resin embedding for electron microscopy at a low relative humidity”. Stain
Technol., 52:5
•
Since epoxy resin is hygroscopic, extreme care should be taken to prevent contamination by water.
•
Accurate measurement of accelerators.
•
Complete mixing without incurring air into the mixture (Use a PTFE or glass rod for at least 20
minutes).
Preparation of Embedding Mixtures
It is essential to mix the components of viscous embedding medium (Araldite + Epon replacements) very
thoroughly to obtain uniform polymerization. Inadequate mixing is one of the main causes of sectioning
problems with epoxy resins. The simplest way to obtain good mixing is to warm the resin, the hardener, and
the containers to be used for stirring at 60°C. A uniform mixture is obtained in a few minutes by this method
and no special stirring apparatus is required. The warm components also are easily poured from their
containers for measurement. It is best to prepare the complete mixture just before use but if necessary it can
be stored at 4°C in a bottle with a well fitting cap for several weeks, or for many months at -20°C. During
infiltration of the specimens, the embedding mixture is kept at room temperature and warmed to 60°C.
Caution: when working with chemicals it is wise to always wear gloves and work under a fume hood
Reference:
Glauert, A.M. Epoxy Resins: An update on THeir Selection and Use. Microscopy and Analysis, September
1991, pp. 15-20.
DMP-30 and DMAE vs BDMA:
BDMA proves to be a much better choice for an accelerator than DMP-30. It is much less viscous, has a
longer shelf life, and offers better penetration of the tissue. When using BDMA in place of DMP-30 more
uniform embedding is obtained. Now it has been shown that the same holds true for BDMA vs. DMAE. It is
highly recommended that when choosing an embedding medium and its corresponding accelerator you
consider using BDMA in place of DMP-30 and DMAE. We have now made available all of our epoxy resin
(Araldite and Embed) kits that come with DMP-30 or DMAE., as standard, BDMA as an alternative to them.
Please note that in order to achieve optimum results when using BDMA in place of DMP-30 the
following proportions should be used:
DMP-30: 1,5-2% added to the final volume
BDMA: 2,5-3% added to the final volume
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SCIENCE SERVICES
Epo-Fix
Embedding Media Kits
RT
Especially formulated for Material Science !
Epo-Fix is an old product that has existed only in the metallographic field
until now. After extensive testing in our labs we have found that it is a
perfect embedding media for a majority of material specimens.
Until now, LR White has been the most commonly used embedding media
for materials due to the block hardness it offered, but there are difficulties
associated with LR White. For instance, the adherance to the specimen is
poor and additives are required to improve its adherance. Another major
problem is the inability to orient the specimen correctly in order to cut you
block afterwards. This is due to the fact that LR White needs to be oxygen
starved and gelatin capsules (vertical) are recommended by the
manufacturer for use. Another problem is that polymerization time in the
oven can take over 24 hours.
Epo-Fix remedies all of these problems. It has very good sectioning properties, good adherance to the
sample, very low viscosity, easy curing in molds (no orientation problems), and quick cure times.
Epo-Fix Technical Data:
A two component epoxy resin, characterized by negligible shrinkage during curing and good mechanical
properties in its hardened state. It is particularly suited for embedding hard samples of complicated shape
and for vacuum impregnation. Samples such as paper, silicon, ceramics, metals, wafers, and chips are
suitable to be used with Epo-Fix. It has low viscosity, low vapour pressure, good wetting properties, and
minimal shrinkage. It hardens at room temperature without significant generation of heat in approximately 8
hours (no damage from heat or pressure), and at increased temperatures, 60°C, the hardening time is
reduced to 2 hours. It should be noted that if you place Epo-Fix in a desiccator during curing you will
eliminate any air bubbles.
Product Code
1232
Epo-Fix kit includes
1kg resin
120g hardener
15 cups
15 stirrers
1 x 5ml and 20ml syringes
Quetol 651 – NSA
Product Code
14640
Epo-Fix is available as a kit and the components
can be purchased separately as well.
0-5°C
Kit consists of
225ml Quetol 651
450ml NMA
450ml NSA
50ml DMP-30
A water miscible epoxy resin used for both light and electron
microscopy. Its low viscosity permits easy infiltration and
embedding, and yields light-colored blocks.
Kushida, H. et al., (1986) Pro. 11th Int. Cong. Electron
Microsc., p. 2177. Kyoto
Quetol and the Addition of Water
The addition of 1% water to the epoxy resin Quetol increases the labeling intensity of the sample. The
significant decrease of the curing temperature of the epoxy resin may assist in preservation of antigens.
Water may also reduce the cross-linkage of the resin allowing more antigen to be available to the antibodies.
The modified Quetol resin is an option for use in immunocytochemistry studies.
Refer to:
Andre R. Abad, (1992). Medium Temperature Epoxy Resin for Immunocytochemistry: Quetol 651 with Water. Microsc. Res. and Techn. 20:274-280.
13
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SCIENCE SERVICES
Embedding Media Kits
EMbed-812
RT
Electron Microscopy Sciences' replacement for the discontinued Epon812. Produces the same results in terms of preservation, handling,
sectioning, and staining as the old Epon-812.
Product Code
14120
•
•
•
•
Kit consists of
450ml EMbed-812
450ml DDSA; Specially Distilled
450ml NMA
50ml DMP-30
Hayat, M. A. Principles & Tech. for EM. Second Edition (1981)
Mascorro, J.A. and Kirby, G.S. Physical characteristics of "old" Epon 812 and various Epon-like
replacements. Proc. 44th Ann. Meet. Electron. Microsc. Soc. Am., pp.222-223 (1986)
Mascorro, J.A. and Kirby, G.S. Novel Epoxy/Anhydride Alternatives for Biological Electron
Microscopy: Physical and Performance Characteristics of EMbed 812 and LX 112 in combination
with NSA/NMA/DMAE. Proc. 47th Ann. Meet. EMSA., pp. 1000-1001 (1989).
Mascorro, J.A. and Kirby, G.S. Viscosity Characteristics and Hardening Rates for EMbed 812 and LX
112 with alternative Anhydride and Catalyst Choices. Proc. 49th Ann. Meet. EMSA. (1991).
EMbed-812/ DER 73
RT
This mixture is less viscous than the final embedding mixture of EMbed-812.
Product Code
14130
UltraBed Kit
Kit consists of
2x225ml DER 736
450ml EMbed-812
450ml NMA
50ml DMP-30
RT
TECH
A Newly Introduced Low Viscosity Epoxy Embedding Kit. A Modification of the Dr.
Spurr’s Formula for Biological, Material, and Mineralogical Specimens
The kit consists of two components, equal amount, very low viscosity, (~65cps), in
convenient dispenser top bottles. To prepare the Embedding Resin, just mix equal
amounts of the two components. The resin readily infiltrates into specimen and
polymerizes to a clear, hard block overnight at 60°C
•
•
•
Convenient – by weight just disperse equal amount of the A and B that you
need, and mix together
Save time – Mixed resin can be used for both infiltration and embedding
Less Hazardous – All components are shipped together as Non- Hazardous
substances
Kit comes with 100 ml each component A and component B
Product Code
14310
Description
UltraBed Low Viscosity Kit
Content
2x100ml
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SCIENCE SERVICES
Araldite 502
Embedding Media Kits
RT
Luft's Formula. Polymerization takes place overnight so blocks can be sectioned the next day. J. Biochem.
Biophy. Cytol. 9, 409 (1961)
Product Code Kit consists of
13900
450ml Araldite 502
450ml DDSA; Specially Distilled
50ml DMP-30
Araldite 6005
RT
Also known as American Araldite. The blocks are slightly harder than those produced by Araldite 502.
Product Code
13920
Kit consists of
450ml Araldite 6005
450ml DDSA; Specially Distilled
100ml BDMA
225ml DBP
Araldite, Embed 812 (Epon-812)
RT
Mollenhauer Epon-Araldite Formula. For hard blocks and high image contrast. Blocks are easily sectioned.
Stain Tech., 39,11 (1964)
Product Code
13940
Kit consists of
450ml Embed-812
450ml Araldite 502
450ml DDSA; Specially Distilled
50ml DMP-30
Durcupan, Water Soluble
RT
A water soluble embedding medium for EM based on an aliphatic polyepoxide.
A Fluka A.G., Buchs. Switzerland-Registered Trademark.
Product Code
14020
Kit consists of
Kit 260g for a minimum of 10
embeddings
Durcupan ACM, Epoxy Resin
RT
An aromatic polyepoxide; a colorless resin of relatively low viscosity, with virtually no shrinkage.
Product Code
14040
15
Kit consists of
Kit 1200ml
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SCIENCE SERVICES
Embedding Media Kits
Low Viscosity, as reported by Dr. Spurr
RT
TECH
Provides excellent penetration for embedding biological tissues and
rapid infiltration. Easy to prepare. The hardness is adjusted by
changing the proportion of the flexibilizer (DER 736). The blocks
have good trimming and sectioning qualities. Thin sections are tough
under the electron beam.
Spurr, A.R., (1969) J. Ultrastruct. Res. 26, 31-42
Product Code
14300
Kit consists of
450ml NSA
225ml ERL 4221
225ml DER 736
25ml DMAE
DER 332-732
RT
Three different mixtures: one for relatively soft blocks, another for harder blocks for collagenous tissue, and a
tougher one if required.
Lockwood, W.R. Anatomical Record, 150, 129 (1964)
Product Code
14000
Kit consists of
2x225ml DER 332
225ml DER 732
450ml DDSA; Specially Distilled
50ml DMP-30
Polyurethane Media
Crystal Clear™ - Water Liquid Plastics
RT
The Crystal Clear™ Series of polyurethane resins were designed for applications that require absolute
clarity. These are low viscosity resins that cure at room temperature with negligible shrinkage to a hard
plastic. Cured products are UV resistant and are not brittle. Applications include potting, encapsulating,
making prototype models, lenses, object duplicating etc.
Ordering:
Each kit consists of 1 gallon resin A and adequate curing agent B
Technical Overview:
Product Code
24200
24204
24206
Description
Crystal Clear 200
Crystal Clear 204
Crystal Clear 206
Pot Life
10 min.
120 min.
180 min.
Full Cure
16 hours
48 hours
48 hours
Casting Thickness
1.27–7.62cm
7.62–15.24cm
> 15.24cm
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SCIENCE SERVICES
Embedding Media Kits
Freezing Media
Tissue-Tek® O.C.T. Compound
Sakura Finetek - This is a well known water soluble glycol’s and resins compound
that provides an excellent specimen matrix for cryostat sectioning at temperatures of
-10°C and below. It leaves no residue during the staining procedure.
Cat. No.
62550-01
62550-12
Tissue-Tek
4583
4583
Description
O.C.T. Compound
O.C.T. Compound
Pack
118ml
12/cs
Tissue Freezing Medium; TFM™
An embedding matrix for frozen sections. TFM™ is an ultra pure formulation of water-soluble glycols and
resins that provides a solid bond between the tissue and the object holder with the following features:
•
•
•
•
•
Less Ice Artifacts: TFM's reduced water content minimizes freeze-fracturing.
Less Curling: TFM™ allows you to pick up flat serial sections with ease.
Freezes Faster: TFM™ freezes very fast and offers better turn-around time.
Completely water soluble: TFM™ reduces tissue dislodging.
Now available in 5 colors: clear, yellow, green, red and blue.
Product Code
72592
72593
72592-Y
72593-Y
72592-G
72593-G
72592-R
72593-R
72592-B
72593-B
Description
Tissue Freezing Medium,
Tissue Freezing Medium,
Tissue Freezing Medium,
Tissue Freezing Medium,
Tissue Freezing Medium,
Tissue Freezing Medium,
Tissue Freezing Medium,
Tissue Freezing Medium,
Tissue Freezing Medium,
Tissue Freezing Medium,
clear
clear
Yellow
Yellow
Green
Green
Red
Red
Blue
Blue
Content
118ml
12x118ml
118ml
4x118ml
118ml
4x118ml
118ml
4x118ml
118ml
4x118ml
Cryogenic-Gel
It is a highly viscous, water-soluble embedding medium for frozen sections that "stays put". The gel is
packaged in a pump-dispenser bottle that can instantly dispense the gel in any position. The dispenser has a
built-in "guillotine" that cleanly severs the gel from the pump spouts and prevents any "stringy"
medium from pulling away when the bottle is removed. To use, simply dispense gel on a room
temperature block-holder. Use a cold heat extractor to form a base layer. Dispense additional
gel. Place the tissue on top of the gel. Use a cold heat extractor to freeze tissue and form a
block.
•
•
•
•
17
Replaces the messy "runny" embedding media.
Easy to use.
Product Code
Sticks to where you want it.
62806-01
Will not run – No waste.
62806-06
Description
Cryo-Gel™, 127ml
Cryo-Gel™, 127ml
Pack
each
12/cs
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SCIENCE SERVICES
Embedding Media Kits
Paraffin & Wax
Polyester Wax
A synthetic polyester wax with a very low melting point (37°C). Invented by
Dr. H.F. Steedman, as a ribboning embedding medium. Its main advantage
over paraffin and ester waxes is its low melting point, which reduces tissue
hardening, shrinkage, and eliminates the need for infiltration ovens.
The wax is soluble in most organic solvents, including alcohol, ethers,
esters, ketones and hydrocarbons. The wax is water tolerant, almost
opaque, and sections easily. No electrification of ribbons occurs during
sectioning. Sections of 2 microns and more may be cut at room
temperatures between 10 and 22°C. It is advantageous to keep the wax in
its solid state prior to use.
Product Code
19312
Description
Polyester Wax
Paramat and Paramat Extra
Content
500g
RT
Paramat is the original British blend of paraffin wax and plastic
polymers and it has been used in histology labs throughout the
world for over 20 years. It has been found that when plastic
polymers are added to the paraffin the elasticity of the final block
is greater as compared with paraffin alone. As well the mixture
offers improved tissue penetration, easier ribboning of sections,
reduced tendency to crumble, and improved overall results with
fibrous tissue.
A recent development to Paramat is the addition of a very small
amount of dimethyl sulfoxide (DMSO) which produces
PARAMAT EXTRA. This formulation offers additional
advantages, such as faster penetration of the tissue with a more
homogeneous matrix to support the specimen.
Product Code
19286-01
19286-10
19287-01
19287-10
Description
Paramat
Paramat
Paramat Extra
Paramat Extra
Content
1 kg
10 kg
1 kg
10 kg
Technical Information
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Appearance: Milky-white, pastilles.
Crystalline Structure under the microscope: Needles & Platelets.
Effect of heating (@60°C/100g): Clear melt within max. 16 hrs.
Solubility in Xylene (@20°C/1g in 50ml): Clear solution within max. 16 hrs.
Cutting results for 4 µm sections: Over 75% usable single; over 65% usable serial.
Solubility in Xylene (section): Max. 45 sec.
Melting Point: 56-58°C.
Solidification Point: 58-56°C.
Tears and Vascuoles: None.
Adhesion of sections Very good; protein, to microscope slides: glycerol etc. is not required to hold
the section in place.
Free acid content: Max. 0.01%.
Block Structure: No mottling, no shrinking.
Block Solidification (room Temperature): About 2 hrs.
DMSO content (Paramat Extra-only): Less than 0.1%.
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SCIENCE SERVICES
Embedding Media Kits
Tissue-Tek® V.I.P.™
Sakura Finetek - High-quality paraffin for processing and embedding.
Quality prepared wax - allows optimal sample preparation.
•
•
•
•
•
Allows reduced compression in sections as thin as 2 µm
Melts at 56°C to protect samples from excessive heat
Will not discolor or produce resin precipitate
Small pellets for rapid melting in tissue processors/embedding centers.
Leaves no plasticizer residue to clog paraffin lines
Specifications:
Medium Composition:
Applications:
Additives:
Melting Point:
High Temperature Stability:
Solid Product Range:
Sectioning Range:
Purified paraffin and Synthetic resin blend
Processing/Embedding
None
56°C
65°C
Pellets
Down to 2µm
Product Code
62580-01
62580-08
Description
4005 Process/Embedding
4005 Process/Embedding
Carbowax Polyethylene Glycol
Content
1 kg
8x1kg
RT
H(OCH2CH2)nOH CAS #2532-68-3
An embedding medium for microscopy and histochemistry.
Product Code
Carbowax Average
Molecular
Mass
Density
@20°C
@60°C
19700
PEG 200
viscous liquid
PEG 300
viscous liquid
PEG 400
viscous liquid
PEG 600
viscous liquid
PEG 1000
Waxy
PEG 1450
Waxy
PEG 3350
Waxy
PEG 8000
Flakes
190-210
1.2390 1.0922 set to glass ←65
450ml
285-315
1.1250 1.0928 -15 to -8 4
450ml
380-420
1.1254 1.0930 4 to 8
450ml
570-630
1.1257 1.0930 20 to 25 450ml
950-1050
—
1.0926
—
1.0919
—
1.0926
—
1.0845
37 to 40
1kg
43 to 46
1kg
54 to 58
1kg
60 to 63
1kg
19710
19720
19730
19740
19750
19760
19770
1300-1600
3000-3700
7000-9000
Melting /Freezing Pack
range, °C
Gao, K.X., (1993). Polyethylene Glycol as an Embedment for Microscopy and Histochemistry. CRC Press;
ISBN# 0-8493-4323-2.
19
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SCIENCE SERVICES
Embedding Media Kits
Peel Away Paraffin Embedding Wax
•
•
•
RT
This new paraffin formulation is more translucent and allows for small dermatological and biopsy
specimens to be seen and sectioned easier.
Its lower polymer content is easier to remove with all cleaning agents. (deparaffinization)
Its lower viscosity allows for complete infiltration using routine times established for most tissue
processors.
Product Code
19300
19300-01
19302
19302-01
19304
19304-01
Polyfin™
Description
Melting Point
Melting Point
Melting Point
Melting Point
Melting Point
Melting Point
Content
10 kg/cs
2.5kg
10 kg/cs
2.5kg
10 kg/cs
2.5kg
52-54°C
52-54°C
56-58°C
56-58°C
62-64°C
62-64°C
RT
Polyfin is a mixture of highly refined paraffins and co-polymer alloys in a
convenient wax pellet. This unique formulation provides optimal tissue
support while maintaining exceptional clarity. Our Polyfin™ has been
institutionally tested and found to function flawlessly in pressure/vacuum
fluid flow processors. Its low melting point (55°C) eliminates any tissue
distortion caused by excessive heat during processing. In a recent study
conducted by a Medical Center Histologist, monitored by a CPA firm
using standard blind protocols, POLYFIN™ clearly demonstrated the
least compression of seven popular paraffins.
Product Code
19280-01
19280-08
19280-15
Description
Polyfin™ Embedding Medium
Polyfin™ Embedding Medium
Polyfin™ Embedding Medium
Paraplast Embedding Medium
Content
1 kilo
8x1 kilo
15 kilo
RT
Product Code Description
19214
Paraplast X-tra
19215
Paraplast X-tra
Content
1 kg
8 kg
Paraplast X-tra
Handy pellet form melts rapidly. Melting Point 53-54°C.
Cuts to 2 micron thickness with exceptional ribbon
continuity. Extra compression resistance provides total
support of tissues.
Product Code Description
19216
Paraplast Plus
19217
Paraplast Plus
Content
1 kg
8 kg
Paraplast Plus
Reduces infiltration time by one-third. Cuts to 2 micron
thickness with excellent ribbon continuity. Convenient
pellet form melts rapidly. Melting point 56°C. Double
filtered. No need for filtration before use.
Product Code Description
Content
19218
Paraplast Medium 1 kg
19219
Paraplast Medium 8 kg
Paraplast Medium
Handy pellet form melts rapidly. Melting Point 56°C.
Cuts to 4 micron thickness with excellent ribbon continuity
- no Crumbling or cracking.
Double filtered. No need for filtration before use.
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SCIENCE SERVICES
Embedding Media Kits
Polyester
Styrene Monomer, Polyester Resin
RT
TECH
Very low viscosity; monomeric styrene which penetrates rapidly into the tissues. It is soluble in ethanol and
acetone. The infiltration and embedding can be done by styrene alone, and there is no need to dilute it with
solvents. Polymerization can be done either by UV light (340-400nm) or heat (60°C) within 2-3 days without
benzoyl peroxide added; 24 hours with 1% benzoyl peroxide added as a catalyst.
Product Code
14650
Kit consists of
450ml Monomeric Styrene
100ml Methyl Ethyl Ketone (UV activator)
225ml Dibutyl Phthalate (plasticizer)
225ml n-Butyl Methacrylate
20g Benzoyl Peroxide (catalyst)
Technovit 4000
RT
The low Shrinkage Embedding Medium
Technovit 400 is a fast curing, cold polymerizing, three-component resin, which is based on a modified
polyester, and it is available in the form of powder, liquid I, and syrup II. It is mixed at a ratio of 2:2:1
(powder:liquid I:liquid II). liquid I and liquid II are mixed together first and the powder is mixed in last. The
color is white opaque.
Properties:
Technovit 4000 is distinguished by its low shrinkage when polymerizing and its perfect margin fit. Due to its
excellent flow characteristics, Technovit 4000 guarantees that geometrically demanding samples are
optimally embedded. Its excellent adhesion properties with regard to metal are a guarantee of gapless
embedding of all metal samples. These properties are of particular importance when working with samples
that require good edge definitions.
•
Excellent margin fit
•
Optimum grinding and polishing properties
Applications:
After mixing, Technovit 4000 can be used for casting for approximately 4 minutes and takes approximately 8
minutes to cure.
Product Code Small Kit
14656-01
20g Powder
60 ml Liquid I
40 ml Liquid II
Vestopal ® 310
Product Code Medium Kit
14656-01
750g Powder
500ml Liquid I
250ml Liquid II
Product Code Large Kit
14656-01
1500g Powder
1000ml Liquid I
500ml Liquid II
0-5°C
This resin has a fine grain and sections are easy to stain. It is characterized by rapid penetration and
polymerization and does not show uneven polymerization. It hardens without shrinkage and the sections are
stable under the electron beam.
Product Code
14700
21
Kit consists of
450ml Vestopal 310 (Formerly W)
10ml Cobalt napthenate
25ml t-Butyl perbenzoate
Ryter, et al., J. Ultrastruct. Res., 2:200 (1958)
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SCIENCE SERVICES
Embedding Media Kits
Other
Diethylene Glycol Distearate (DEGDS/DGD)
RT
TECH
DGD is a removable embedding medium (for thin and thick sections) that provides embedment free sections.
Good for immunolabeling and high resolution light microscopy. Ethanol and n-butyl alcohol are used as
dehydrating agents.
Product Code
14010
Kit consists of
450ml DGD
450ml Ethanol
450ml n-Butyl alcohol
DGD Embedding Kit
Advantages:
Thin or thick embedment free sections can be obtained.
Sections are easy to cut and ribbons are easily obtainable.
Enhances the integrity of the specimen as it floats on the
water.
Sections produce interference colors according to its
thickness.
Gach (Glutaraldehyde/Carbohydrazide)
Product Code
15920
Kit consists of
10x10ml 50% EM grade
Glutaraldehyde
10x1.5g Carbohydrazide
Hard-Plus Resin-812
RT
RT
A water and lipid-retaining embedding polymer for EM.
Excellent preservation of lipids and ultrastructure.
Hechman, C.A. et al. (1973) J. Ultrastruct. Res. 42,156.
TECH
A newly formulated resin that enables the processing of specimens that have a tendency to wrinkle and be
problematic.
In general, Hard Plus Resin-812 and its WPE numbers (weight per epoxide) do not vary much from batch to
batch and it also has good stability under the electron beam and it stains well with heavy metals.
Furthermore, the viscosity of the Hard-Plus Resin-812 is lower than the original Epon-812, making infiltration
of the tissue more complete. For processing and embedding, it can be used exactly the same way as Epon812, but normally, it is not necessary to use intermediate solvents, such as propylene oxide, hence,
infiltration can immediately follow with absolute alcohol. Polymerization is normally at 60ºC for 24 – 48 hours.
Longer times or higher temperatures (70ºC or more) will give a harder block.
For larger samples (>1x1mm3) the use of a intermediate solvent is recommended.
Product Code
14115
Kit consists of
5x50g Hard-Plus Resin-812
5x50g Hardener Hard-Plus
5x 2.5ml Accelerator
Hard-Plus Resin-812 Kit
Hard-Plus Resin-812 is supplied only as a
premix kit. All components are pre-measured,
and all you have to do is combine them in the
right order and mix thoroughly. Each kit contains
five separate mini-kits, making approximately
100g each.
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| 22
SCIENCE SERVICES
Embedding Capsules and Holders
Embedding Capsules and Holders
Capsules; Embedding, Size 00, 8mm inner diameter
Polyethylene embedding capsules with 1x1mm face at the tip of a truncated pyramid, each with a hinged cap.
Both EMS and orignal BEEM® capsules are available.
EMS Embedding Capsules
Product Code
Quantity
70000
100/pk
70010
500/pk
70020
1000/pk
Original BEEM®
Product Code
70000-B
70010-B
70020-B
Capsules
Quantity
100/pk
500/pk
1000/pk
Embedding Capsule; Flat
Added to our extensive line of embedding capsules is our flat bottom
capsules, which are made from polyethylene, and are 8mm (just like the
standard 00 capsules) in diameter.
Product Code
70021
Description
Flat Bottom Embedding Capsules
Quantity
500/pk
Embedding Capsule Holder
Fabricated from polyethylene. It accommodates 15 embedding capsules size "00". The whole capsule is
exposed to allow for even heat and/or UV light distribution during polymerization.
Sturdy, long lasting, and stackable. Measures: 5"(L) x 3"(W) (127mm x76mm)
Product Code
70022-01
70022-04
70022-06
70022-12
Description
EMS Capsule Holder
EMS Capsule Holder
EMS Capsule Holder
EMS Capsule Holder
Quantity
each
4/pk
6/pk
1 doz.
EMS and BEEM® Embedding Capsules Size 3 (small)
These capsules, made from polyethylene, produce blocks with a 5.6mm outer diameter and a pyramid tip.
Comes complete with a hinged cap.
Both EMS and Orignal BEEM® capsules are available.
EMS Embedding Capsules
Product Code
Quantity
69911-01
100/pk
69911-05
500/pk
69911-10
1000/pk
23
Original BEEM®
Product Code
69910-01
69910-05
69910-10
Capsules
Quantity
100/pk
500/pk
1000/pk
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SCIENCE SERVICES
Embedding Capsules and Holders
BEEM® Embedding Capsules; Special Shapes
Available in two specially shaped capsules which are designed to overcome commonly encountered
embedding problems; such as, small or thin elongated specimens and powder samples. These capsules are
also good for centrifuging precipitated matters and allows for less trimming.
The bottle neck capsule produces a 7.9mm OD block with a hemihyperpoloid (rather than faced) tip.
The conical capsule produces a 7.9mm OD block with a conical (rather than faced) tip
Product Code
69912-01
69912-05
69912-10
69913-01
69913-05
69913-10
Description
BEEM® Capsule Bottle Neck Tip
BEEM® Capsule Bottle Neck Tip
BEEM® Capsule Bottle Neck Tip
BEEM® Capsule Conical Tip
BEEM®Capsule Conical Tip
BEEM® Capsule Conical Tip
Quantity
100/pk
500/pk
1000/pk
100/pk
500/pk
1000/pk
BEEM® Capsule Holders
These holders are used to hold capsules upright during the resin embedding stage. The cavities (22) allow
for illumination from the bottom of the holder for specimen orientation. Available in two sizes: one holds size
"00", conical, and bottle neck capsules; the other holds size #3 capsules.
Product Code
69916-01
69916-06
69916-12
69917-01
69917-06
69917-12
Description
BEEM® Capsule Holder size "00"
BEEM® Capsule Holder size "00"
BEEM® Capsule Holder size "00"
BEEM® Capsule Holder size 3
BEEM® Capsule Holder size 3
BEEM® Capsule Holder size 3
Quantity
each
6/pk
12/pk
each
6/pk
12/pk
Measures 5½"(L)x4"(W)x7/8"(H) 138x100x12mm
The Opaque BEEM® Capsule Holder
If microwaving or UV polymerizing of your embedding media these
opaque BEEM® capsule holders are perfect. Made from
Polypropylene. All embedding capsules size "00" fit it.
Product Code
69915-01
69915-06
69915-12
Description
BEEM® Capsule Holder, Clear
BEEM® Capsule Holder, Clear BEEM® Capsule Holder, Clear Quantity
each
6/pk
12/pk
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| 24
SCIENCE SERVICES
Embedding Capsules and Holders
BEEM® Block Storage
A two tone color coded plastic box with insert, which allows for the
storage of up to 10 blocks in the numbered cavities. Measures:
21/3"(L)x1¾"(W)x½"(H) 57x45x12.5mm
Capsule Block Storage
For the storage of 15 capsule
blocks in numbered cavities.
Product Code
69922-01
69922-05
69922-10
Description
BEEM® Block Storage
BEEM® Block Storage
BEEM® Block Storage
Quantity
each
50/pk
100/pk
Product Code
69923-01
69923-05
69923-10
Description
Capsule Block Storage Box
Capsule Block Storage Box
Capsule Block Storage Box
Quantity
each
50/pk
100/pk
Capsules; Gelatin
Natural, hard gelatin capsules. To be used as an embedding mold for
water miscible resins, or resins which need to be cured by transmitted
light. Gelatin capsules dissolve or disintegrate within 5 minutes when
immersed in a 0.5% HCl solution at 36-38°C, within 2 minutes in water
at 37°C or in artificial gastric juice.
Product Code
70101
70100
70110
70102
70103
70104
70105
Size
000
00
0
1
2
3
4
Overall inches
1.029
0.917
0.835
0.748
0.688
0.610
0.547
Length mm
26.14
23.30
21.20
19.00
17.50
15.50
13.90
Body inches
0.376
0.322
0.289
0.261
0.239
0.219
0.199
Diameter mm
9.55
8.18
7,34
6.63
6.07
5.56
5.05
Volume ml
1.37
0.95
0.68
0.50
0.37
0.30
0.21
Large Gelatin Capsules
Our large size gelatin capsules are ideal for storing either delicate or small items. It is also suitable for use as
an embedding mold for paraffin or paraplast. The capsules come complete with a locking ring. A variety of
sizes are available.
Product Code
70114
70115
70116
70117
70118
25
Size
13
12
11
10
7
Length mm
24
33
38
52
66
Diameter mm
13
13
19
22
22
Volume oz.
01.08.09
01.04.09
01.02.09
1
11.02.09
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SCIENCE SERVICES
Embedding Capsules and Holders
Gelatin Capsule Holder
Made from durable polyethylene. It accommodates 15 gelatin capsules size "00". The whole capsule is
exposed to allow for even heat and/or UV light distribution during
polymerization. Measures: 5"(L) x 3"(W) (127mm x 76mm).
Product Code
70120-01
70120-04
70120-06
Description
Gelatin Capsule Holder
Gelatin Capsule Holder
Gelatin Capsule Holder
Quantity
each
4/pk
6/pk
BEEM® Specimen Block Storage System 2000®
•
•
•
Are your bench-tops and drawers crowded with petri dishes
and pillboxes full of various sized specimen blocks?
Have you been spent hours to look for a block for resectioning?
Do you wish you could neatly catalog, file and store all of those
blocks in one convenient place?
If you answer yes to any of those questions, now you can organize and
safely store all of your specimen blocks with the newly introduced
BEEM® Specimen Storage System 2000®
The system consists of four newly designed Blocklock® storage
modules (#69956, #69957, #69958, #69959) and a Covered Mounting Panel (#69950) that fits in a standard
"D-Type" 3-ring binder (optional) – A 1-inch binder will accommodate two Covered Mounting Panels, and 1.5inch binder accommodates three Panels.
Each Blocklock module has an adhesive backing which when exposed permits it to be permanently mounted
on any grease-free surface.
All Blocklocks can be inserted in a Plastic Protective Box (#69956-B & 69958-B, #69957- B, and 69959-B) or,
by utilizing the adhesive feature, mounted in combination with other Blocklock modules on the BEEM®
mounting panel (#66950). This new Storage System 2000 permits blocks size "00", size "3", and BEEM® flat
embedment to be indexed, combined under one cover along with any associated data sheets, and stored
upright in 3-ring binders, on the shelf, at your reach for ready reference.
This system is infinitely expandable to high or low volume block storage. The choice is yours!
Capsule Blocklock Storage Modules
Incorporate an innovation that allows blocks to snap into cavities
specially designed to retain them so they cannot be inadvertently
dislodged. Number and letter identify each cavity. Available with three
configurations:
•
Model 2006 measures 2 7⁄8"L x 2"W x ¼"H (74 x 50 x 8mm);
accommodates 12 size "00" blocks
•
Model 2007 measures 4 5⁄8"L x 3 ½"W x 5⁄16"H (117 x 90 x
8mm), accommodates 40 size "00" blocks.
•
Model 2008 measures 2 7⁄8"L x 2"W x ¼"H (74 x 50 x 6mm),
accommodates 20 size"3" capsule blocks.
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| 26
SCIENCE SERVICES
Embedding Capsules and Holders
BEEM® Blocklock Storage Modules Model 2009
Is the same features as Capsule Blocklocks. Measures 2 7⁄8"L x 2"W x
¼"H (74 x 50 x 8mm), accommodates 20 BEEM® flat blocks. (BEEM®
flat embedding mold, #70904-01`
NOTE: Do to the rectangular shape of a BEEM® flat embedment; it
must be inserted into the cavity in a Blocklock module #69959 as
follows:
Introduce the front of the block at a slight angle into the rear of the
cavity and gently push it forward until it nests in the recess.
BEEM® Blocklock Storage Boxes
Heavy-duty, two tone plastic boxes are supplied with indexed,
translucent labels, which can be applied to the top or bottom of the box.
•
•
•
Box #69906-B and #69908-B are the same size and are twotone blue/clear. Overall measurement: 3"(L) x 2 1⁄8"(W) x ½"(H)
(78 x 54 x 13mm).
Box #69957-B is two-tone blue/clear. Overall measurement: 4
¾"(L) x 3 ¾"(W) x½"(H) (120 x 94 x 13mm)
Box #69959-B is two-tone black/clear. Overall measurements
are the same as #69956-B and #69958-B
BEEM® Mounting Panel
With clear cover is designed to accept both Blocklock Storage Modules
and BEEM® original Dial- A-Grid (#71148) and Block Storage Modules
(#69922), which can be attached to the panel using double sided
adhesive tape. Grid storage boxes from other manufacturers may be
attached this way also if they do not exceed 3⁄8" in height.
The panel inside measures: 10 5⁄8"(L) x 7½"(W) x 3⁄8"(H) (270 x 190 x
12mm). The overall measurement is 11 3⁄8"(L) x 8 ¾"(W) x 9⁄16"(H)
(290 x 225 x 22mm)
Ordering: Note: Blocklocks and their Storage Boxes are sold
separately.
Product Code
69950
69956
69956-B
69957
69957-B
69958
69958-B
69959
69959-B
69960-10
69960-15
27
BEEM®
2010
2006
2006B
2007
2007B
2008
2008B
2009
2009B
Description
Mounting Panel with Clear Cover
Blocklock, Size "00", 12 Positions
Blocklock Storage Box for #69956
Blocklock, Size "00", 40 Positions
Blocklock Storage Box for #69957
Blocklock, Size "3", 20 Positions
Blocklock Storage Box for #69958
Blocklock Flat embedment, 20 positions
Blocklock Storage Box for #69959
"D-Type" 3-Ring Binder, 1inch
"D-Type" 3-Ring Binder, 1.5 inch
Pack
each
each
each
each
each
each
each
each
each
each
each
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SCIENCE SERVICES
Embedding Capsules and Holders
Plastic Flat Embedding Mold
When the mold and the box BE8 are used together, the set allows for the use of oxygen-prohibited
embedding materials (Ie: LR White) to be polymerized in a flat orientation. These molds are reusable. Each
cavity measures 12mm(L) x 5mm(W) x 3.5mm Deep. Overall mold measures: 94 x 56 x 14mm.
•
•
•
Injected molded polyethylene.
Resistant to most embedding materials.
Easy peeled-off the blocks.
Mold fits perfectly inside the cocoon box BE8 (64300-8); see page 41
Product Code
70905-01
70905-12
Description
EMS Flat Embedding Mold
EMS Flat Embedding Mold
Quantity
each
12/pk
BEEM®™ Capsule Press
The Capsule Press is designed to facilitate the removal of blocks from the
capsule without any damage to the block tip.
PLEASE NOTE: FOR BEEM®* capsule size #3, an adapter is required.
Product Code
69920-00
69920-03
Description
BEEM® Capsule Press
Capsule Press Adaptor
Pack
each
each
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SCIENCE SERVICES
Embedding Capsules and Holders
Easy-Molds™
A newly designed mold which is convenient and offers better embedments. This tray-and-mold in one unit
offers a unique specimen block that no other mold can.
Features:
1.
2.
3.
4.
5.
6.
7.
Trays-and-molds are self standing. There is no need for a
holding tray.
Easy Orientation of Specimens. The tip of each mold is only
0.3mm thick and is designed to be transparent. This facilitates the
orientation of your specimen prior to polymerization.
They form a Universal Block. Many microscopists have to embed
large specimens. The Easy Mold tip forms a flat block face which is
wide enough for large specimens. This wide surface helps to
prevent air bubbles being trapped around the specimen during
polymerization. In addition, a positive guide form on the block offers
an accurate position for clamping.
Free Airflow for Even Polymerization. Easy Mold trays raise the
molds away from the surface of the oven, allowing air of a constant
temperature to circulate freely and evenly around the specimen tip
during the hardening process.
Sealing of Capsules. If you are using Acrylate or other volatile
embedding mediums (LR White, GMA, HPMA), the molds can be
sealed by placing another Easy Mold on the top, and by applying a
little pressure you can seal the capsule completely.
Easy to Remove the Polymerized Block. Due to the softness of
the bottom of the mold, to release the block simply push the bottom
of the mold with your thumb and your block will just pop out. There
is no need for razor blades or a Capsule Press!
Convenient Storage and Identification of Sectioned Blocks.
Each cavity on the Easy Mold is numbered, and data can be written
on the trays. Used Easy Molds can be used for the storage of
blocks which have already been sectioned, and filed away by
stacking one tray on top of another. There is no need for block
storage boxes.
Technical Specifications:
Each tray contains 2x10 block molds and measures 87x66mm.
They are available in two sizes:
•
•
29
Size 5.6mm diameter, which
corresponds to gelatin and BEEM®
capsules size 3, and forms a block
face of 1.6mm.
Size
8mm
diameter,
which
corresponds to gelatin and BEEM®
capsule size "00" and forms a block
face of 3mm.
Product Code
69930-05
69930-25
69930-50
69931-05
69931-25
69931-50
Description
Easy Mold, Size 3
Easy Mold, Size 3
Easy Mold, Size 3
Easy Mold, Size "00"
Easy Mold, Size "00"
Easy Mold, Size "00"
Pack
5 Trays
25 Trays
50 Trays
5 Trays
25 Trays
50 Trays
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SCIENCE SERVICES
Embedding Molds
Embedding Molds
Science Services Exclusive! Best price! Outstanding quality!
These molds are made of green silicone rubber. They are flexible, reusable and offer a greater cemical
resistance compared with other molds. After polymerization the blocks can be released without difficulty by
simply flexing the mold.
Flat Embedding Mold
Green or translucent silicone rubber mold. 21 rectangular numbered compartments allowing easy orientation of tissues.
Mold size: 70 x 70 x 7 mm thick.
Specimen Block: 5.2 x 13.8 x 3.2
Product Code
306
306-10
Description
Flat Embedding mold Green
Flat Embedding mold , Translucent
Pack
Each
Each
Small Embedding Mold
Green or translucent silicone rubber mold, with 15 unmarked cavities. Specimen
block will produce trapezoidal shaped tips for each block, for easy trimming.
Mold Size: 68 x 68 x 8mm
Specimen Blocks :6.4 x 11.7 x 5mm
Product Code
306-4
306-40
Description
Small embedding mold, Green
Small embedding mold, Translucent
Pack
Each
Each
Round Embedding Mold
Green silicone rubber mold. Round shape for very large sections (diameter
92mm)
Product Code
369
Description
Round embedding mold, Green
Pack
Each
Cochlea Embedding Mold
Only available from Science Services!
10 compartments 10 x 8 x 6mm. Green silikone rubber. Specially developed to
allow for the embedding of a complete guinea pig cochlea.
Product Code
SCI-G
Description
Cochlea embedding mold, Green
Pack
Each
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SCIENCE SERVICES
Embedding Molds
Double End Flat Mold
This mold will produce pre trimmed trapezoidal flat block shaped on both ends.
Made of green or translucent silicone rubber, flexible and reusable. Capacity 20
numbered cavities.
Mold size: 89 x 62 x 7mm
Specimen Block:14 x 6 x 4mm
Product Code
306-3
306-30
Description
Double end flat mold, Green
Double end flat mold, Translucent
Pack
Each
Each
Large Flat Embedding Mold with Trapezoids
This green silicone rubber mold has 24 self-numbering cavities. Will produce
trapezoidal shaped tips for easy trimming. This innovative mold also contains 3
special cavities for multiple specimen embedding. Each cavity can hold 3 large
sample.
Mold Size: 114 x 73 x 7mm
Specimen Block: 6.4mm x 14mm x 3.9mm
Product Code
305-5
Description
Large flat embedding mold
With trapezoids, Green
Pack
Each
Large Flat Embedding Mold with rectangles
Made of green or translucent silicone rubber, flexible and reusable. Contains 20
rectangular compartments, producing pre trimmed blocks, and 8 rectangular
depressions.
Mold size: 112 x 75 x 7 mm
Specimen Block: 6.4 x12.8 x 3.8mm
Product Code
306-2
306-20
31
Description
Large Flat embedding Mold
with rectangles, Green
Large Flat embedding Mold
with rectangles, Translucent
Pack
Each
Each
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SCIENCE SERVICES
Embedding Molds
Capsule Molds
Our molds are made from the strongest possible silicone rubber providing extremely high tear resistance.
They are all reusable and produce embedding blocks of numerous shapes and sizes with a wide variety of
tips. We also offer a mold which produces blocks to be used for re-embedding. Choose the one that best
suits your embedding needs!
Product Code
70150
70160
70161
Description
Capsule Molds Type A: 10 cavities, 8mm
diameter body, 5mm diameter tip, 11mm
height, tapered tip.
Capsule Molds Type B: 12 cavities, 8mm
diameterbody, 5mm diameter tip 16mm
height, tapered tip.
Capsule Molds Type C: 12 cavities cylindrical
shapes, 8mm diameter 16mm height, flat tip.
Pack
each
each
each
The Embedding Capsule Substitute:
This 24 cavity mold produces a block similar to the embedding
capsule block but with the following advantages:
•
•
•
•
•
the rubber heats more evenly and retains the heat better
than in plastic.
Resin polymerizes better in silicone rubber than in
polypropylene or polyethylene
It is easier to see inside the well when centering the specimen
in the tip.
It is easier to remove the block from the well. There is no need
for razor blades or press devices.
The finished blocks have finer tips so when you trim deeper
into the block the face doesn't increase in size rapidly;
therefore, constant retrimming is not necessary
The Mold for Long Tissues:
This mold accommodates longer pieces of tissues (muscle, skin
biopsies, and many plant specimens)
Advantages:
•
•
Easy to embed long specimens and orient them properly. The
tissues lie flat in the well; therefore, no horizontal trimming is
needed.
It is ideal for any specimen that needs immediate sectioning (e.g.
cell monolayer). 24 cavities
Product Code
70166
Description
The Mold for Long Tissues
Pack
each
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SCIENCE SERVICES
Embedding Molds
The Cell Culture Mold:
This mold is designed primarily for embedding cell culture
specimens grown either directly on the bottom of a dish or on the
filter when cross sections of the cells are needed:
1. Cells grown on petri dishes are cut into strips just before the
propylene oxide dehydration step; when Propylene Oxide is added,
the strips lift right off the bottom.
2. When the strips are ready for embedment, they are rolled on an
applicator stick into a wad and gently lowered into the well (the strips
should not float).
Product Code
70167
Description
The Cell Culture Mold
Pack
each
Advantages:
•
•
•
•
•
•
Many cells can be examined in cross section.
There is no need for trimming - sectioning can begin immediately.
The curved side makes the tip very strong in spite of its thinness.
Easy detection of a strip of cells.
The label is placed on the round part so as not to interfere with the specimen.
24 cavities.
Chien Silicone Universal-Mold
A two-directional mold that allows for single embedded specimens to be sectioned both laterally and
transversely . Tissues to be embedded are accurately trimmed and placed so that both sides of the tissue
are against both edges of the cavity tip.
Chien, K., et al. (1985) Proc. Ann. EMSA Meeting
43, 460
Product Code
70140
70141
Description
Chien Silicone Mold
Chien Silicone Mold
Pack
each
10/lot
Small Flat Embedding Mold
Made from clear silicone, the overall measurements are 1.65” x 3” with 12 cavities. Each cavity measures
10mm(L) x 6mm(W) x 4mm (Deep). This mold produces 12 embedding blocks with tapered ends.
Product Code
70903
33
Description
Small Flat Embedding Mold
Pack
each
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SCIENCE SERVICES
Embedding Molds
Double Tapered Ends
A standard flat embedding mold where both ends are tapered. This enables you to embed specimens in both
ends of each casting. Made from white silicone, 21 numbered cavities, each cavity measuring:
14mm(L)x4mm(W)x4mm(D)
Product Code
70870
Description
Double Tapered Ends Mold
Pack
each
Triple Tapered Ends
A new design with 10 triple numbered, tapered tip cavities to save you time and handling. Holds firmly in a
chuck during trimming or sectioning. Made from white silicone, each cavity measures 8mm from tip to center,
5mm deep.
Product Code
70875
Description
Triple Tapered Ends Mold
Pack
each
Flat Embedding Molds
A clear silicone rubber with very high tear strength. It can be reused more times than the conventional
silicone molds. It is resistant to most epoxy resins and its clear base allows positioning of the specimen
lighted from beneath.
Product Code
70900
70901
70902
Description
Standard flat embedding mold,
21 numbered cavities measures:
14mm(L)x5mm(W)x4mm(D).
(Clear silicone)
Same as #70900, but each of the
21 numbered cavities measures:
14mm(L)x5mm(W)x6mm(D).
(White silicone)
Same as #70900, but each of the
21 numbered cavities measures:
14mm(L)x5mm(W)x3mm(D).
White silicone)
Pack
each
each
each
Stepped Microtome Embedding Mold
EMS Embedding Mold is a new mold designed with Michael J. Dykstra
Ph.D. from North Carolina State University Department of Veterinary
Medicine. This Mold produces blocks of the best dimension to be held
securely by Reichert/Leica Ultracut stepped microtome chucks. There are
24 cavities and the depth of each cavity is 4mm which will prevent
breakage of the blocks during razor blade trimming.
Mold Measurements: 3 3⁄8" x 3" (85 x 77mm)
Cavity Measurements: 15mmx 7mm x 4mm deep
Product Code
70907
Description
Embedding Mold-Dykstra
Pack
each
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SCIENCE SERVICES
Embedding Molds
Slide Duplicating Molds
Uses a 1x3" glass slide as a base, and duplicates the slide using the
resin of your choice. Tissue sections are embedded against the flat glass
surface. This duplicated resin slide can be studied under the Light
Microscope, then cut, remounted, resectioned, and studied under the EM.
A three cavity mold, each cavity measuring: 1"(W)x3"(L)x3/32"(D).
Reference & Instructions enclosed
Product Code
70170
Description
Slide Duplicating Mold
Pack
each
Slide Duplicating With Cover Slip Mold
The same design as above 70170 but with the addition of three round
cavities which are 17mm in diameter and 1mm deep.
Product Code
70172
Description
Slide Duplicating w/Cover Slip Mold
Pack
each
Chang Monolayer Molds
Designed by Dr. J.P. Chang for the embedding of a monolayer of cells. This specially designed mold has
proven useful in the processing of frozen sections, ascite cells, fresh imprints, as well as blood and marrow
smears. J. of Ultrastructural Res., 37, 370 (1971).
Product Code
70910
70920
35
Description
Chang Mold 12 cavities, 20x10x1mm
Chang Mold 9 cavities, 20x20x1mm
Pack
each
each
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SCIENCE SERVICES
Embedding Molds
Flat Embedding For Acrylics
No more stickiness with new storage box Acrylic resins are no longer a
challenge for flat embedding. In combination with the Cocoon box, which
withstands temperatures up to 70 Degrees C, and our flat embedding
molds flat embedding of acrylic resins (LR Gold, Lowicryls, etc.) are now
possible. Oxygen is no longer an issue with our new Cocoon Boxes.
These molds can be used for all resins except LR white.
Product Code
70900-CB
70901-CB
70902-CB
70870-CB
Description
4mm Flat Embedding Mold w/Cocoon Box
6mm Flat Embedding Mold w/Cocoon Box
3mm Flat Embedding Mold w/Cocoon Box
Double End Flat Embedding Mold w/ Cocoon Box
Pack
set
set
set
set
BEEM® Flat Embedding Molds
Molded from polyethylene. The critical surfaces of the mold are
transparent, facilitating accurate specimen orientation through bottom
illumination. Molds are reusable and each mold is supplied with a
protective box.
The cavity measures: 12mm(L) x 5mm(W) x 3mm(D)
Overall mold measures: 52mm(L) x 40mm(W) x 5mm(H)
Storage box measures: 57x45x12.5mm
Product Code
70904-01
70904-12
Description
BEEM® Flat Embedding Mold
BEEM® Flat Embedding Mold
Pack
each
12/pk
Tissue Culture Monolayer Embedding Mold: Thompson Embedding Mold
This invention is a mold used for embedding mammalian cells in
monolayer culture in preparation of sectioning for light or electron
microscopy. Tissue culture monolayer embedding molds eliminate the
need for traditional BEEM® capsules. The improved molds can be used to
transfer the majority of cells from the entire surface of a cover slip to avoid
losses of large amounts of cellular information occurring only randomly. In
addition, other problems such as surface blemishes caused by air
entrapment associated with BEEM® capsules are eliminated.
Product Code
70924
70925
Description
Thompson Mold
Thompson Mold
Pack
each
4/lot
Advantages:
•
•
•
•
The mold yields several individual blocks of resin from a single slip-cover.
The resin blocks fit directly into a chuck for flat specimens.
The embedded surfaces are free from blemishes caused by entrapped air.
The mold uses a minimum amount of resin.
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SCIENCE SERVICES
Blue Silicone Rubber
Embedding Molds
RT
Blue Silicone Rubber is extremely high tear, high tensile strength, two components, and tin catalyzed RTV
silicone rubber. It is designed for casting polyurethane foam, polyester and epoxy parts. It is a superior
product with the following advantages over the RTV silicones:
•
•
•
•
•
•
•
•
Extremely high tear strength
Low shrinkage Low viscosity
Excellent chemical resistance
Excellent shelf life
Color coded catalyst
Long pot life Insensitive to inhibition
Variable cure rate
Good dielectric properties
Typical Properties
Uncatalyzed Compound
Color
Viscosity (cps)
Specific Gravity
Working time (hours)
Cured Time (hours)
Shelf Life ( months)
Base
White
50,000 – 70,000
01.10.99
1.5 to 2.5
16 to 18
6
Activator
Blue
300 – 400
Cured Rubber (7 days @ 70°F & 50% R.H.)
Hardness Shore A 6
Tensile Strength (psi)
Elongation (%)
Tear, Resistance (ppi)
Shrinkage (%)
Specific Gravity
Dielectric Strength (volts/mil)
Dielectric Constant @ 100Hz
Dissipation Factor @ 100Hz
Volume Resistivity (ohms/cm)
32±4
525 ± 25
300 ± 25
120 ± 10
0.1
1,09
500
3,3
19
1 x 1015
Ordering: Blue Silicone Rubber comes as a kit consisting of 1 gal (9 lb) silicone base and 1 pt (1lb) blue catalyst.
Product Code
24230-B
37
Description
Blue Silicone Rubber Kit
Pack
1 gal
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SCIENCE SERVICES
Embedding Molds
Clear Silicone Rubber
RT
This silicone cures at room temperature with no shrinkage and offers a unique water clear see through
rubber with excellent tear strength, chemical and heat resistance.
Technical Overview:
Hardness Shore A 6
Color
Pot Life
Demold Time
Specific Volume
Specific Gravity
Mixed Viscosity
Die B Tear Strength
Tensile Strength
Shrinkage
Mixing Ratio Wt. Or Vol.
40
Translucent
60 min.
16 hours
25,8
1,07
35,000cps
120 pli
800 psi
Negligible
100:10 pbw
Curing: allow the mold to cure overnight (at least 16 hours) at room temperature (77°F/25°C) before
demolding. Do not cure rubber where temperature is less than 65°F/18°C. Post Curing the mold will aid in
quickly attaining maximum physical and performance properties. After curing at room temperature, expose
the rubber to 80°C for 2 hours and 100°C for 2 hours. Allow mold to cool to room temperature before using.
NOTE: This clear silicone will cause rubber to yellow when applying post curing.
Ordering: Clear silicone comes as a kit of 1gallon (9 lb) silicone A and 1 pt (.9lb) curing agent B.
Product Code
24234-C
Description
Clear Silicone Rubber Kit
Pack
1 gal
Disposable Flat Embedding Mold
Molded from flexible polyethylene, opaque clear. Disposable. Produces
ten of 0,32x0,95x1,91cm and fourteen of 0,32x0.95x1,91cm blocks.
Product Code
70906-10
Description
Disposable Flat Embedding Mold
Pack
10
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SCIENCE SERVICES
Embedding Molds
Tissue-Tek® Cryomold®; Sakura Finetek
These disposable plastic Cryomolds produce a uniformly shaped flat surface specimen block with tissue
freezing medium (O.C.T.) for frozen sectioning, Standard Cryomold fits directly into Cryobar® freezing wells
of the Tissue-Tek® II microtome/cryostat. Use a Cryomold® adapter to adapt smaller biopsy and
intermediate Cryomold® molds to freezing wells on the Cryobar® unit.
Product Code
62534-25
62534-10
62534-15
Tissue-Tek
4557
4565
4566
Description
Pack
Standard, 25x20x5mm
100/pk
Biopsy, 10x10x5mm
100/pk
Intermediate 15x15x5mm 100/pk
Disposable Base Mold
Our Disposable Base Mold is designed to fit all existing cassettes. Because our base molds are disposable
you save time on cleaning and storing and they offer you less exposure to hazardous chemicals. Packed
125/dispenser box.
Product Code
62352-07
62352-15
62352-24
62352-30
62352-37
Description
Disposable Base Mold 7x7x5mm
Disposable Base Mold 15x15x5mm
Disposable Base Mold 24x24x5mm
Disposable Base Mold 30x24x5mm
Disposable Base Mold 37x24x5mm
Pack
500/pk
500/pk
500/pk
500/pk
500/pk
Peel Away Disposable Embedding Molds
For plastic or paraffin embedding; no block trimming needed. Inside fingers hold ID slip which becomes
embedded in the block for positive permanent identification.
Product Code
70180
70181
70182
70183
70184
39
Description
Truncated Mold, size 8x8mm
Truncated Mold, size 12x12mm
Square Mold, size 22x22mm
Rectangular Mold, size 22x30mm
Rectangular Mold, size 22x40mm
Pack
288/cs
288/cs
288/cs
288/cs
288/cs
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SCIENCE SERVICES
Embedding Molds
Cocoon Box – A Smart Box
The Revolutionary Way for Mailing and Storing Samples
•
•
Boxes - Made from polypropylene, envelope shape, available with or without an inner frame. These
boxes are the safest way for mailing or storing fragile samples. They are impact and shock resistant
With a living-hinge, opaque-clear, reusable and recyclable. The boxes are available in many sizes
and styles to fit your needs. Closing Clips and Security Seals are also available as an option.
Vials – are made from polystyrene and they are tough and crystal clear and come with a friction lid.
Cocoon Boxes are also available to accept these vials for safe storing and/or mailing valuable
samples.
Ordering:
BE3, BE3A, BE3B, and BE3D
Box BE3 comes with inner frame to form a cocoon; BE3A is without an
inner frame offering more room inside of the box; BE3B comes with 8
round cavities, each 0.94”x0.62”H (24x16mm) for storing Vials DB20;
BE3D comes with 6 round cavities each 1.29”x0.62”H (33x16mm) for
storing vials DDB30
Catalog
64300-3
64300-3A
64300-3B
64300-3D
Outside dimensions
135x99x18mm
135x99x18mm
135x99x18mm
135x99x18mm
Inside dimensions
102x65x15mm
110x71x15mm
110x71x15mm
110x71x15mm
Quantity
10/pk
10/pk
10/pk
10/pk
BE4, BE4A and BE4B
Box BE4 comes with an inner frame to form a cocoon; BE4A is without
an inner frame for more room inside of the box; BE4B comes with 6
round cavities, each 0.94”x0.47”H (24x12mm) for storing vials DB10
Catalog
64300-4
64300-4A
64300-4B
Outside dimensions
114x94x14mm
114x94x14mm
114x94x14mm
Inside dimensions
83x60x9mm
89x74x10mm
89x74x10mm
Quantity
10/pk
10/pk
10/pk
BE6
Large size with an inner frame. The yellow box (BE6Y) is accepted by
the US Postal service AS-IS. No need for envelopes or other containers.
This box is also available in clear (BE6C).
Catalog
64300-6Y
64300-6C
Outside dimensions
245x170x18mm
245x170x18mm
Inside dimensions
210x135x15mm
210x135x15mm
Quantity
10/pk
10/pk
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SCIENCE SERVICES
Embedding Molds
BE8 and BE8A
BE8 comes with an inner frame. BE8A - The-Envelope-Mailer - comes
without the inner frame.
Catalog
64300-8
64300-8A
Outside dimensions
94x65x14mm
94x65x14mm
Inside dimensions
62x31x10mm
64x31x10mm
Quantity
10/pk
10/pk
BE9, BE9A, and BE9B
BE9 comes with single inner frame. BE9A comes with two compartments
and BE9B comes with 4 compartments.
Catalog
64300-9
64300-9A
64300-9B
Outside dimensions
210x100x18mm
210x100x18mm
210x100x18mm
Inside dimensions
180x67x15mm
180x32x15mm
87x32x15mm
Quantity
10/pk
10/pk
10/pk
Vials DB10, DB20, and DB30:
Vial DB10 is designed for the Cocoon Box BE4B (64300-4B); Vial DB20
is designed for the cocoon Box BE3B (64300-3B); and Vial DB30 is
designed for the Cocoon Box BE3D (64300-3D).
Catalog
64302-10
64302-20
64302-30
Outside dimensions
24x12mm
24x16mm
33x16mm
Inside dimensions
19x7.5mm
18x13mm
28x13mm
Quantity
12/pk
12/pk
12/pk
Locking Clips
Used to secure the cocoon boxes. Clips snap together to keep cocoon
boxes securely lock.
Catalog
Description
Quantity
64301-10
Locking Clips
100/pk
41
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SCIENCE SERVICES
Embedding Supplies
Embedding Supplies
Liquid Blocker–Super PAP Pen
The Newest Staining Guard Pen
New and improved. The Super PAP Pen outperforms the original PAP Pen. The Super PAP Pen may be used
at temperatures as high as 50°C. (The old style PAP Pen worked only up to 30°C). With this high temperature
feature, one can now use the Super PAP Pen to perform the peroxidase-anti-peroxidase method, ABC
method, and Fluorescent antibodies method with satisfactory results.
Product Code
71310
71312
Description
Super PAP Pen, Large
Super PAP Pen, Small
Pack
each
each
Fro-Marker
A wide tipped pen designed for use with frozen sections. The pen provides a sticky membrane which when
applied to the slide and onto which the section is placed it eliminates the section from falling off, moving, or
wrinkling on the slide during immunostaining procedures. The membrane is stable up to 110°C and is
suitable for vigorous applications such as in-situ hybridization.
Product Code
71313-10
Description
Fro-Marker
Pack
each
Para-Marker
A wide tipped pen, designed for use with paraffin sections. The pen provides a sticky membrane to the glass
slide which not only prevents paraffin sections from falling off but also keeps the sections flat on the slide.
Product Code
71313-20
Description
Para-Marker
Pack
each
Butler Block Trimer
A precision made device to aid in hand trimming resin tissue blocks to the desired
shape for ultramicrotomy. The knurled knife guide swivels to help the trimming
blade to cut the sides of the block. May be utilized with any stereomicroscope with
epi-illumination. The trimmer is supplied with a standard chuck to accommodate
the block size 00. A flat embedding mold adapter is available as an option. (Stain
Technology, Vol. 49, No 4: pp. 129-132, 1974)
Product Code
69945-01
Description
BUTLER Block Trimming
Pack
each
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SCIENCE SERVICES
Embedding Supplies
Aluminum Chucks
Machined from solid Aluminum. They come with set screws and a hex key.
Product Code
70174-10
70174-14
70174-20
Description
ABH-JB Chucks for Hacker, A/O Reichert rotary and
Autocut, JB-4 and JB-4A, LKB Ultratomes and Shandon
Hypercut. Stem measures 11 x 11 mm
ABH-LBK Huxley Chuck. Stem measures 11 x 25 mm
ABH-JBA Chuck with a three point recessed block holder
attachment: for LKB Histo Range and Reichert Super-Cut in
addition to microtomes using ABH-JB chucks (#70174-10).
Stem measures 11 x 11 mm
Pack
each
each
each
Ultramicrotomy Chucks
Precision machined from aluminum. These special chucks are made to accommodate small specimen
blocks, which have been polymerized in Eppendorf tubes. In addition, we also have chucks that hold all sizes
of flat embedding blocks (fitting all Leica and RMC instruments) ,and chucks for LKB instruments.
Product Code
69990
69991
69992
69993
Description
Flat embedding Chuck
LKB Chuck
Eppendorf Chuck for RMC
Eppendorf Chuck for LEICA
Pack
each
each
each
each
EBH-2 Block Holder
•
•
•
•
Molded from hard plastic.
Easy to store block holders attached to Aluminum chucks (ABH)
Frosted surface for easy marking.
Large center hole prevents bubble formation.
Product Code
70175-50
43
Description
EBH-2 Block Holder
Pack
50/pk
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SCIENCE SERVICES
Embedding Supplies
Tissue-Tek® Mold Release, Concentrate
Use this solution to coat base molds to ensure complete separation of embedding
paraffin from the mold. The working solution is prepared by adding 5ml of
Concentrate to 95 ml of ethyl alcohol or isopropyl alcohol. Molds are dipped in
solution and dried prior to use.
Product Code Tissue-Tek
62530-01
4141
62530-12
4141
Description
Mold Release
Mold Release
Pack
118ml
12/cs
Liquid Release Agent
A hydrosoluble mold-release agent for use in the flat embedding of
micro-organisms, and as a replacement for the coating of glass slides
to produce a smoother, even surface. The reference below describes
the selection and precisely oriented sectioning of single cells by light
microscopy, prior to examination by EM.
Reymond, O.L. & Picket-Heaps, J.D., (1983) J. Microscopy, 130, 179.
Product Code
70880
Description
Liquid Release Agent
Pack
100ml
TFE-like Spray / Mold Release Spray
A TFE release agent, dry, lubricant. No discernible transfer, no migration, no build-up.
Contains: Isopropanol, Poly-TFE, 1,1-Dichlorofluoroethane and Carbon Dioxide.
Contains no silicones, CFC's or Methyl Chloroform. Its working temperature is up to
140°F.
Product Code
72619
Description
PTFE-Like Spray
Pack
15 oz.
Para/Gard™
A paraffin repellent for countertops as well as equipment. Just spray and wipe away.
No more paraffin build up around your work area. ParaGard™ is a pleasant smelling,
non-toxic spray which is safe for use on metal, wood, and plastic surfaces. Comes in
a 4 oz. pump bottle.
Product Code
64140-01
64140-06
19280-15
Description
Para/Gard™
Para/Gard™
Polyfin™ Embedding Medium
Content
118ml
6x118ml/cs
15 kilo
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SCIENCE SERVICES
Embedding Devices
Embedding Devices
Tissue Embedding Center
Introducing a new Tissue Embedding Center with
the latest in circuit board and component
technology.
Uniquely
designed
with
microprocessor temperature sensors, a single
console which maximizes space utilization, highcontrast viewing areas and multifunctional work
stations. The entire unit is encased in a durable,
non-heat conductive housing.
Features:
•
•
•
•
•
•
•
•
•
•
•
•
•
Microprocessor controlled for dependability and performance.
Single module design.
Low and flat work-surface which facilitates operator efficiency.
User friendly membrane switches.
4-liter capacity paraffin reservoir which minimizes refilling frequency.
Heated forceps warmer and illuminated paraffin dispenser enhance user friendliness.
Warming oven with removable shelf and double hinged lid for convenient access to preheated base
mold.
Wax bath complete with drainage shelf, debris screen, and hinged lid.
Heated work area which provides a flat working surface with the excess paraffin draining under the
surface into the wax bath; complete with a hand and foot switch for activating the dispensing head.
Generous illumination.
The cold plate offers 170 sq. in. (1100cm2) of compressor cooled working surface with removable
stainless steel drainage tray beneath.
Tactile membrane touch-pad for easy temperature setting and monitoring.
Full one year warranty.
Specifications/ Dimensions:
Overall
Hgt of Work Surface
Wax Reservoir
Wax Bath
Warming Oven
Cold Plate
Weight: Overall
Temp Ranges:
Wax Reservoir
Work Surface
Wax Bath
Cold Plate
Power Supply:
Fuse Rating:
23.75"(L)x31.5"(W)x11"(H) (60x81x28cm)
Workstage 2.75"(7cm) above countertop
.75"(L)x4.75"(W)x4"(D) (19.5x12x9.5cm)
10.25"(L)x8.5"(W)x1.75"(D) (26x21.5x4cm)
6.5"(L)x7.5"(W) x min 2.5" max 6" (14 - 16.5cm)
11.75"(L)x14.5"(W) (29.5x36.6cm)
72 lbs (33kg)
40° - 70°C +/-2°C
40° - 70°C +/-5°C
40° - 70°C +/-2°C
ambient to -5°C
Overall 120 VAC, 50 Hz
Power plug 5 amps
Ordering:
Product Code
62300
45
Description
Tissue Embedding Center
Pack
each
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Embedding Devices
SCIENCE SERVICES
THE EMS Tissue Embedding Center
The Histo-Pro 150 offers superior operating convenience for tissue embedding processes in a modular
ergonomic design.
Features and Benefits:
•
•
•
•
•
•
•
•
•
•
Two Module system.
All stainless steel work surfaces
User friendly proprietary PAT interface
Large cold plate and cassette bath.
Fully programmable time and temperature
Adjustable paraffin flow rate
Self draining forceps holder.
Auto Defrosting cold plate
Four Liter paraffin tank.
Ability to turn on automatically
System Specifications:
Histo Pro 150H Hot Module
Histo Pro 150CS Cold Module
Capacity:
Paraffin Tank:
Mold Oven:
Capacity:
Cold Plate Area:
Temperature Range
Cassette Bath:
4 liters
7” (17.5 cm)Wide x 7” (17.5 cm)
Deep x 2.25” (5.7 cm) Tall
8” (20 cm) Wide x 11” (28 cm)
Long x 2.1”(5.3 cm) Deep
User Selected Temperature Ranges:
Paraffin Tank:
40-70° C
Mold Oven:
40-70° C
Cassette Bath:
40-70° C
Forceps Holder:
40-70° C
Work Surface:
40-70° C
15.8” (40 cm) x 14” (35 cm)
-10°C - +10°C
Physical Characteristics: Width:
16”(40 cm)
Depth:
22.5”(57.2 cm)
Height:
12.5”(32 cm)
Weight:
35 lbs (16 kg)
Electrical Characteristics: Voltage:
230 Volts AC
Power:
200 Watts Max
Physical Characteristics: Width:
20”(51 cm)
Depth:
22.5”(57.2 cm)
Height:
12.5”(32 cm)
Weight:
35 lbs (16 kg)
Electrical Characteristics: Voltage:
230 Volts AC
Power:
800 Watts Max
Ordering:
Product Code
63120
Description
HistoPro 150 Tissue Embedding System
Pack
each
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SCIENCE SERVICES
Embedding Devices
Paraffin Section Mounting Bath
An Electrothermal – Top of the Line Mounting Bath
This Mounting Bath Offers
•
Comfort and easy manipulation of the sections.
•
Constant clear visibility.
•
Coated aluminum housing.
Product Description
•
Easy manipulation of the sections avoids problems with wrinkles, folds and distortions.
•
2.5liter (capacity) one-piece black interior is easy to clean and provides constant clear visibility to the
sample.
•
Aluminum exterior with polypropylene lid is durable and easy to clean.
•
Easy to replace the heating elements.
•
Built-in controller regulates the temperature range from ambient to 75ºC
•
The control panel is front mounted included on/off switch and temperature knob
•
A clear light indicates "Power On". A red light warns of over-temperature. Amber light indicates
"Heater On".
•
Excess or damage surfaces can be easily removed by skimming a filter paper over the water
surface.
•
A built-in safety device shuts off the energy supply to the heater in the event of an overheat condition
such as boiling dry.
Specifications:
•
Electrical (50/60Hz): 230V, 240W (#63222-20)
•
Chamber Dimensions: 8.7" (I.D.) x 3" (H) (22cm (I.D.) x 7cm (H))
•
Overall Dimensions: 13 " (O.D.) x 5 " (H) (33cm (O.D.) x 13cm (H))
•
Weight: 6 lb (2.7 kg)
Ordering:
Product Code
63222-20
47
Description
Paraffin Section Mounting Bath, 230V
Pack
each
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SCIENCE SERVICES
Embedding Devices
Paraffin Wax Dispenser Electrothermal
The most Reliable Wax Dispenser in the Market!
•
•
•
Provides immediate "Wax on Tap"
For use in pathology laboratories
Thermally insulated tap heater with independent
temperature contol eliminates wax solidification
Product Description
•
Anodized black interior stove enameled black and
white aluminum exterior and black metal lid are
durable and easy to clean
•
Capacity of 1 gallon (4.5 liters)
•
Built in controller provides temperature control
from ambient to 75°C
•
A non-drip swivel lever with replaceable filter
makes the dispensing of wax easy.
•
Tap heater maintains temperature within the tap to
keep the wax molten.
Safety
•
•
Safety over-temperature at 105°C
Non-resettable thermal fuse
Product Specifications
Model
Chamber Capacity
Overall Dimensions
Chamber Dimensions
Weight: Shipping
Electrical 50/60 Hertz
EMS#
MH8523B8
4.5 l
30 x 28cm Dia
15 x 15cm Dia
4.5kg
230 Volts, 175 Watts
62839-20
Ordering:
Product Code
62839-20
Description
3,75 l Paraffin Dispenser, 230 V
Pack
each
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SCIENCE SERVICES
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Science ServiceS
Science Services GmbH
Unterhachinger Str. 75
Science Services GmbH
81737 Munich, Germany
Your Partner for · Microscopy · Ultracentrifugation · General Lab Supplies
Goerzer Strasse 70
81549 Munich, Germany
T +49 (0)89 18 93 668
T +49 (0)89 15 980 280
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