Biotage Lysera Cryo
®
Cooling Unit
User Manual
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Data herein has been verified and validated. It is believed adequate for the intended use of
the instrument. If the instrument or procedures are used for purposes over and above the
capabilities specified herein, confirmation of the validity and suitability should be obtained;
otherwise Biotage does not guarantee results and assumes no obligation or liability. This
publication is not a license to operate under, or a recommendation to infringe upon, any
process patents.
This product is warranted to be free from defects in material and workmanship for a
period of TWO YEARS from the date of delivery. Biotage will repair or replace and return
free of charge any part which is returned to its factory within said period, transportation
prepaid by user, and which is found upon inspection to have been defective in materials
or workmanship. This warranty does not include normal wear from use; it does not apply
to any instrument or parts which have been altered by anyone other than an employee of
Biotage nor to any instrument which has been damaged through accident, negligence,
failure to follow operating instructions, the use of electric currents or circuits other than
those specified on the plate affixed to the instrument, misuse, or abuse. Biotage reserves
the right to change, alter, modify, or improve any of its instruments without any obligation
whatever to make corresponding changes to any instrument previously sold or shipped.
THE FORGOING OBLIGATION IS IN LIEU OF ALL OBLIGATIONS AND LIABILITIES INCLUDING
NEGLIGENCE AND ALL WARRANTIES OF MERCHANTABILITY OR OTHERWISE, EXPRESSED
OR IMPLIED IN FACT OR BY LAW, AND STATE OUR ENTIRE AND EXCLUSIVE LIABILITY
AND BUYERS EXCLUSIVE REMEDY FOR ANY CLAIM OF DAMAGES IN CONNECTION
WITH THE SALE OR FURNISHING OF GOODS OR PARTS, THEIR DESIGN, SUITABILITY FOR
USE, INSTALLATION, OR OPERATION. BIOTAGE WILL IN NO EVENT BE LIABLE FOR ANY
SPECIAL OR CONSEQUENTIAL DAMAGES WHATSOEVER, AND THEIR LIABILITY UNDER
NO CIRCUMSTANCES WILL EXCEED THE CONTRACT PRICE FOR THE GOODS FOR WHICH
LIABILITY IS CLAIMED.
This product has been engineered for safety; however, basic safety precautions and
common sense must always be demonstrated when using any electrical product.
- DO NOT attempt to modify any part of this product.
- DO NOT allow the machine to be submerged in any liquid.
- DO NOT use in any setting other than an indoor laboratory.
- DO NOT plug power cord into an incorrect outlet or subject it to an incorrect voltage.
- Use this product only for its intended purpose.
- DO NOT use attachments not recommended by the manufacturer.
- DO NOT operate the product if it is damaged in any way.
- Keep this product away from heated surfaces.
- DO NOT modify the plug or cord that is provided. If the plug will not fit the outlet, have the proper outlet installed by a qualified electrician.
- DO NOT operate the product with the safety ground disconnected.
WARNING: Reduce the risk of unintentional starting; make sure the speed switch is in the OFF position before plugging in the motor.
WARNING: Damaged or worn power cords should be repaired or replaced immediately by a qualified electrician.
WARNING: Improper connection of the equipment can result in a risk of electric shock.
Overview
1.
5.
2.
6.
3.
7.
4.
8.
1. Lid
2. Airflow Control Knob
3. Insulated Cryo Hose
4. Biotage® Lysera Cryo
Lid
5. Cold Air Outflow
6. Compressed Air Input
7. Compressed Air Hose
8. External Thermometer
The Lysera Cryo Cooling Unit consists of the following:
Description
Quantity
Biotage® Lysera Cryo Cooling Unit
Insulated Cryo Hose
Biotage® Lysera Cryo Lid
External Thermometer
Compressed Air Hose
Tool Kit
1
1
1
1
1
1
User Manual
1
Installation
Removing the Biotage® Lysera Lid
1.
Locate Lid Hinges.
2.
Lift Lid and remove 4 screws using the provided Allen Key.
Remove Lid
3.
Replace with Biotage® Lysera Cryo Lid (Black)
Align black Cryo lid onto the Lysera.
4.
Replace the four screws and tighten using the provided Allen Key.
Installation
Install the External Thermometer
1.
Thermocouple
probe wire
Couple clip
Thermometer probe
Position the Thermocouple Probe under the Biotage® Lysera lid as
shown, using the Couple Clip to secure the thermocouple probe
wire under the Lysera lid.
2.
Place remaining Couple Clips as shown.
Guide the thermocouple probe wire through
the clips.
3.
Plug the thermocouple probe wire into the
External Thermometer.
NOTE: The Digital Thermometer has been
preset to Celsius with alarms to be triggered
if temperatures reach below -10oC or over
70oC.
Installation
Install Intake and Output Hoses
NOTE: Compressed ISO8573 Class 2 or better air is required to use the
Cryo unit: Total oil < 0.1 mg/m3, vapor pressure dew point < -50oC.
1.
Connect the hose from air supply to the rear of
the Cryo unit.
Note: An optional hose with a “quick connect”
coupling has been included with the Cryo. An
additional fitting may be required to connect
the hose to the air supply.
2.
Connect the straight end of the Insulated Cryo
Hose to the Cryo Unit.
3.
Connect the 90o elbow end of the Insulated
Cryo Hose to the Biotage® Lysera Lid.
Recommended Dry Air Guidelines
• Obtain a high-pressure cylinder of Medical Air. Medical Air is a blend of
nitrogen and oxygen that contains virtually no traces of oil or water vapor.
OR
• Between an air compressor and the Cryo Unit inlet, use a refrigerated air
dryer or desiccant dryer. These systems are specified in the lowest dew
point that can be reached.
• DO NOT use inlet pressure below 55 PSI or above 120 PSI.
Operation
Liquid Nitrogen
Pre - Cooling
WARNING: Wear eye, face, hand and skin protection when working with
liquid nitrogen. Operate in a well ventilated area.
PRE-COOLING - follow pre-cooling procedure before homogenizing
samples.
NOTE: Do not load samples into the Biotage® Lysera processing chamber
prior to pre-cooling.
- Purge air lines by running dry air for 30
1.
seconds at the MAX setting.
- Turn knob to the Off position.
2.
Add 0.5 L or 16 oz. of liquid nitrogen to the
chamber, fill to 3 inches (7.6 cm) below the
top of the chamber.
NOTE: The copper coils in the chamber must
be completely covered by liquid nitrogen.
3.
Close the lid of the Cryo unit and turn the air flow control knob
counterclockwise to begin the flow of air into the processing
chamber.
Pre-cool the Biotage® Lysera processing chamber to 0oC.
WARNING: DO NOT pre-cool the processing chamber below 0oC.
Operation
Liquid Nitrogen
Homogenizing Samples
1.
Ensure the knob of the Cryo is in the off
position and all hoses are connected
correctly.
2.
- Load samples into the Biotage® Lysera.
- Install Finger Plate
- Set the desired time, speed dwell and
number of cycles on the Lysera.
3.
Close the Biotage® Lysera lid.
4.
Add 0.5 L or 16 oz. of liquid nitrogen
to the chamber of the Cryo unit, fill to
3 inches (7.6 cm) below the top of the
chamber.
NOTE: The copper coils in the chamber must
be completely covered by liquid nitrogen.
CAUTION: 55-120 PSI is the recommended air pressure for use with the
Cryo. Pressure above 120 PSI could damage internal components.
Operation
Liquid Nitrogen
Homogenizing Samples
5.
Turn Airflow control knob counter clockwise
to begin cooling.
6.
Allow air to flow until the External
Thermometer reads 0oC.
7.
Once the temperature has reached 0oC,
press RUN on the Biotage® Lysera to begin
homogenization.
8.
When the cycle has ended:
- Turn off compressed air supply
- Turn off Cryo
- Open the lid of the Lysera and
remove samples.
CAUTION: Allow liquid nitrogen to
evaporate before attempting to move the
Cryo unit.
WARNING: Avoid direct skin contact with Cryo and Lysera components
after exposure to liquid nitrogen. Always use protective gloves.
Operation
Dry Ice
Pre-Cooling
Liquid nitrogen will provide the best results. However, dry ice
and alcohol may be used.
WARNING: Wear eye, face, hand and skin protection when working with Dry
Ice. Operate in a well ventilated area.
PRE-COOLING - follow pre-cooling procedure before homogenizing samples.
NOTE: Do not load samples into the Biotage® Lysera processing chamber
prior to pre-cooling.
- Purge air lines by running dry air for 30
1.
seconds at the MAX setting.
- Turn knob to the Off position.
2.
- Add 0.5 L (16 0z.) Of dry ice to the Cryo
chamber.
- Add 0.5 L (16 oz.) of ethanol to the Cryo
chamber and the mixture sit for five
minutes to allow the coil to cool.
NOTE: The copper coils in the chamber
must be completely covered by the alcohol
and dry ice.
3.
Close the lid of the Cryo unit and turn the air flow control knob
counterclockwise to begin the flow of air into the processing
chamber.
Pre-cool the Biotage® Lysera processing chamber to -5oC.
WARNING: DO NOT pre-cool the processing chamber below -5oC.
Operation
Dry Ice
Homogenizing Samples
1.
Ensure that the knob of the Cryo is in the
off position and all hoses are
connected correctly.
2.
- Load samples into the Biotage® Lysera.
- Install Finger Plate
- Set the desired time, speed dwell and
number of cycles on the Lysera.
3.
Close the Biotage® Lysera lid.
4.
- Add 0.5 L (16 0z.) Of dry ice to the Cryo
chamber.
- Add 0.5 L (16 oz.) of ethanol to the Cryo
chamber and the mixture sit for five minutes
to allow the coil to cool.
NOTE: The copper coils in the chamber must
be completely covered by the alcohol and
dry ice.
CAUTION: 55-120 PSI is the recommended air pressure for use with the
Cryo. Pressure above 120 PSI could damage internal components.
Operation
Dry Ice
Homogenizing Samples
5.
Turn Airflow control knob counter clockwise
to begin cooling.
6.
Allow air to flow until the External
Thermometer reads -5oC (23oF)
7.
Once the temperature has reached -5oC,
press RUN on the Biotage® Lysera to begin
homogenization.
8.
When the cycle has ended:
- Turn off compressed air supply
- Turn off Cryo
- Open the lid of the Biotage® Lysera and
remove samples.
CAUTION: Allow the dry ice to evaporate
and dispose of remaining alcohol before
attempting to move the Cryo unit.
WARNING: Avoid direct skin contact with Cryo and Lysera components
after exposure to dry ice. Always use protective gloves.
Trouble Shooting
Problem
Possible Cause
The cooling system
is not supplied with
compressed air.
Cold air is not flowing
into the Biotage® Lysera.
- The airflow nozzles are
obstructed.
- The air purity is lower
than recommended.
One or several outflows One or more nozzles are
do not work.
defective.
The airflow holes are
obstructed.
System is not
performing optimally
The outflow control
valves or regulator are
defective.
Action
1. Check that the air
source is open.
2. Check that there are
no leaks in the hoses.
1. Wait for the units to
completely de-frost.
2. Ensure the air purity
is 99% and the water
content <5ppm.
Contact Technical
Support
1. Wait for the units to
completely de-frost.
2. If problem persists,
contact technical
support.
Contact technical
support.
DO NOT: attempt to service the Cryo Cooling Unit in a manner other than those discussed
in this manual. For any issue that is unsuccessfully corrected using this guide, please contact your authorized dealer or call Biotage 1-Point Support Tel: +1 800 446 4752.
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order@biotage.com
Support Tel: +1 800 446 4752
Support Tel: +46 18 56 59 11
Outside US: +1 704 654 4900
Support Fax: + 46 18 56 57 11
us-1-pointsupport@biotage.com
eu-1-pointsupport@biotage.com
Part Number: UI375
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