Instructions For Use
JS-4.3 Swinging-Bucket Rotor
For Use in Beckman Coulter
Avanti JXN-26
Avanti J 26S XP Series,
Avantil J-26 XP Series,
and Avanti J-HC Centrifuges
PN J-TB-050EB
September 2016
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
JS-4.3 Swinging-Bucket Rotor
PN J-TB-050EA (September 2016)
Copyright ©1992–2016 Beckman Coulter, Inc.
All rights reserved. No part of this document may be
reproduced or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or
otherwise, without prior written permission from
Beckman Coulter, Inc.
Avanti, Aerosolve, Beckman Coulter, the stylized logo, and
Microfuge are trademarks of Beckman Coulter, Inc. and
are registered in the USPTO.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Find us on the World Wide Web at:
www.beckmancoulter.com
Printed in the U.S.A.
Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to use this equipment. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.
Alerts for Warning, Caution, Important, and Note
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.
IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed.
Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.
NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
Safety Information for the JS-4.3 Rotor
This rotor was developed, manufactured, and tested for safety and reliability as part of a
Beckman Coulter centrifuge/rotor system. Their safety or reliability cannot be assured if used in a
centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centrifuge that has been
modified without Beckman Coulter’s approval.
This rotor is warranted for 7 years (see the warranty at the back of this publication).
Beckman Coulter recommends that you retire the rotor from use at the end of the 7-year warranty
period to prevent the possibility of a rotor mishap resulting from material fatigue.
Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that such fluids are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) viruses, HIV (I–V), atypical mycobacteria, and certain systemic fungi—further emphasize the
need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this rotor without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
PN J-TB-050EB
iii
Safety Notice
Safety Information for the JS-4.3 Rotor
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more
than one level of protection.
Dispose of all waste solutions according to appropriate environmental health and safety guidelines.
The rotor and accessories are not designed for use with materials capable of developing flammable
or explosive vapors. Do not centrifuge such materials (such as chloroform, ethyl alcohol) in or
handle or store them near the centrifuge.
If glass tubes break, remove the glass very carefully from the adapter, bucket, or cavity. If all the
glass particles are not contained in the bucket or cavity, be careful when examining or cleaning the
centrifuge gasket and chamber as glass particles may be embedded in their surfaces.
Inspect the rotor once a month, especially inside cavities, for rough spots or pitting, white powder
deposits—frequently aluminum oxide—or heavy discoloration. If any of these signs are evident, do
not run the rotor. Contact your Beckman Coulter representative for information about the Field
Rotor Inspection Program and the rotor repair center. To reduce the potential for corrosion, clean
buckets or carriers thoroughly immediately following a tube or well plate breakage. Be sure to
remove all glass particles from buckets or carriers.
Components or accessories designed for other rotors may cause rotor mishap if used in this rotor.
Use only components and accessories that have been designed for use in this rotor. The safety of rotor
components and accessories made by other manufacturers cannot be ascertained by Beckman Coulter. Use
of other manufacturers’ components or accessories in the rotors may void the rotor warranty and should
be prohibited by your laboratory safety officer. If tubes, microplates, or other labware made by
manufacturers other than Beckman Coulter are used, reduce rotor speed to prevent breakage. The
strength of glass and plastic tubes can vary between lots, and will depend on handling and usage;
we highly recommend that you pretest labware in the rotor using water samples to determine
optimal operating conditions. Scratches (even microscopic ones) significantly weaken glass tubes.
The rotor must be run with a full complement of buckets and/or multiwell plate carriers attached
to the yoke. You can run four buckets, four multiwell plate carriers, or two buckets and two carriers
(with like components loaded opposite each other). If only two buckets are loaded with blood bags,
bottles, or modular disk adapters, the other two buckets should contain at least a minimal “blank”
load (for example, empty modular disk adapters) to achieve optimal results and to avoid
rotor imbalance.
The maximum allowable run speed (4300 RPM) listed in the rotor specifications is for operation
when all conditions are within the standard specifications (using buckets). Maximum allowable run
speed when using Micro Plus multiwell plate carriers is 3250 rpm. Do not overload the rotor without
reducing the speed (see SPEED DERATING). Failure to derate will reduce the safe useful life of the rotor.
Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins
in scratches and may open fissures in the rotor with continued use.
If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor.
Apply appropriate decontamination procedures to the centrifuge, rotor, and accessories.
iv
PN J-TB-050EB
Safety Notice, iii
Alerts for Warning, Caution, Important, and Note, iii
Safety Information for the JS-4.3 Rotor, iii
JS-4.3 Swinging-Bucket Rotor, 1
Specifications, 1
Description, 3
The Rotor, 3
Buckets and Accessories, 3
Modular Disk Adapters, 3
Bottle Adapters, 9
Blood Bag Cups, 9
Bucket Covers, 9
Aerosolve Canisters, 9
Micro Plus Multiwell Plate Carriers, 9
Installation, 11
The Rotor Yoke, 11
Buckets or Carriers, 12
Loading the Rotor, 13
Working with Physiological fluids, 13
Symmetric and Balanced Loading, 13
Using Buckets, 17
Assembling Modular Disk Adapters, 17
Loading Tubes in Adapters, 18
Loading Adapters in the Buckets, 19
Using Bottles in Adapters, 19
Using Blood Cups, 19
Bucket Covers, 20
Using Aerosolve Canisters, 22
Using Micro Plus Multiwell Plate Carriers, 23
Operation, 24
Speed Derating, 24
Temperature Limits, 26
Run Procedure, 26
Temperature Control, 27
At the End of the Run, 28
Care and Maintenance, 28
Inspection, 28
Maintenance, 29
Cleaning, 30
The Rotor Components, 30
Buckets, 31
Micro Plus Plate Carriers (Including Base and Pad), Blood Bag Cups,
and Bucket Covers, 31
Modular Disk Adapters, 31
Aerosolve Canisters, 32
v
Decontamination, 32
Sterilization and Disinfection, 32
Tube Breakage, 33
Aerosolve Canisters, 33
SST or Corvac Tubes, 33
Returning a Rotor, 35
Supply List, 36
Replacement Rotor Parts, 36
Supplies, 36
Beckman Coulter, Inc.
J Series Swinging-Bucket Rotor Warranty
Related Documents
vi
1
The Micro Plus Multiwell Plate Carrier, 10
2
Rotor Yoke and Centrifuge Drive Spindle Hub, 12
3
Examples of Correctly and Incorrectly Loaded Buckets and Carriers, 14
4
The Bails (Vertical Supports) of the JS-4.3 (and GH-3.8) Modular Disk Adapters
and the J6 Series Adapters are Not the Same, 16
5
Examples of the Effects on the Horizontal Swing of Buckets Caused
by Proper and Improper Weight Distribution, 17
6
Assembling a Modular Disk Adapter, 18
7
Installing an Adapter in a Bucket, 19
8
Blood Bag Loading Procedures, 21
9
Proper Placement of O-ring, 21
10
Securing Bucket Cover Latches, 22
11
The Micro Plus Multiwell Plate Carrier, Base, Pad, and Deep-well Plate, 23
12
Lubricating Pivot Pin/Bucket Contact Areas, 29
vii
viii
1
Relative Centrifugal Fields for the JS-4.3 Rotor, 2
2
Modular Disk Adapters, Bottle Adapters, and Cups Available for the JS-4.3
Rotor, 4
2
Table 2 Modular Disk Adapters, Bottle Adapters, and Cups Available for the
JS-4.3 Rotor (continued), 5
2
Table 2 Modular Disk Adapters, Bottle Adapters, and Cups Available for the
JS-4.3 Rotor (continued), 6
3
Beckman Coulter Tubes and Bottles for the JS-4.3 Rotor, 6
4
Labware for Use with Micro Plus Carriers, 10
5
Recommended g-Force Limits to Minimize Breakage of Certain Glass Tubes
and Bottles Made by Other Manufacturers in the JS-4.3 Rotor, 25
6
J2-HC Centrifuge Temperature Compensation Settings for the JS-4.3 Rotor, 28
ix
x
JS-4.3 Swinging-Bucket Rotor
Specifications
Maximum speed (buckets) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4300 RPM
Maximum speed (Micro Plus carriers) . . . . . . . . . . . . . . . . . . 3250 RPM
Critical speed rangea . . . . . . . . . . . . . . . . . . . . . . . . . . 400 to 1450 RPM
Maximum solution density. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 g/mL
Relative Centrifugal Fieldsb at maximum speed
(see Table 1 for RCF at other speeds)
using buckets (rmax = 204 mm) . . . . . . . . . . . . . . . . . . .
rmin
rav
rmax
1
1. Axis of Rotation
4220  g
using Micro Plus carriers (rmax = 163 mm) . . . . . . . . . 1924  g
Conditions requiring speed reductions . . . . . . . . . . see Speed Derating
Number of buckets or carriers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Available tubes and bottles. . . . . . . . . . . . . . . . . . . . . . . . . . see Table 3
Maximum load allowed in each bucket at rated
speed (excluding weight of bucket and cover). . . . . . . 1000 grams
Maximum load allowed in each Micro Plus carrier
at rated speed (excluding weight of carrier) . . . . . . . . . . 500 grams
Maximum rotor capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 liters
Approximate acceleration time, fully loaded rotor . . . . . . . 1 1/2 min
Approximate deceleration times
max setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 1/2 min
“0” setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 1/2 min
Weight of fully loaded rotor
(buckets with covers) . . . . . . . . . . . . . . . . . . . . . . . . . 12.3 kg (22.2 lb)
Rotor yoke material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . stainless steel
Bucket material. . . . . . . . . . . . . . . . . . . . . . . . . . . . . anodized aluminum
Carrier material . . . . . . . . . . . . . . . . . . . . . . . . . . . . anodized aluminum
a. The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass. Passing thrugh the critical
speed range is characterized by some vibration.
b. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (r2) to the standard acceleration of
gravity (g) according to the following formula: RCF = r/g — where r is the radius in millimeters,  is the angular velocity in radians per second
(2  RPM /60), and g is the standard acceleration of gravity (9807 mm/s2). After substitution: RCF = 1.12r (RPM/1000)2
PN J-TB-050EB
1
JS-4.3 Swinging-Bucket Rotor
Specifications
Table 1 Relative Centrifugal Fields for the JS-4.3 Rotora
Relative Centrifugal Field (g)
Rotor Speed
(RPM)
Buckets
(204 mm
Micro Plus Carriers
(163 mm)
4300
4000
3750
4220
3660
3210
3500
3250
3000
2800
2410
2060
Don’t run above 3250 RPM
1924
1643
2750
2500
2250
1730
1430
1160
1380
1141
922
2000
1750
1500
913
700
514
729
558
410
1000
500
228
57
182
46
a. Entries in this table are calculated from the formula RCF = 1.12r (RPM/1000)2 and
then rounded to three significant digits.
Relative Centrifugal Field (RCF)
JS-4.3 Rotor
4500
4000
3500
RCF (x g)
3000
2500
Buckets
2000
Micro Plus
1500
1000
500
0
0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
Speed (RPM)
2
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Description
Description
Specific information about the JS-4.3 rotor is given here. Information common to this and other rotors is
contained in the manual Rotors and Tubes for J Series Centrifuges (publication JR-IM), which should be
used with this manual for complete rotor and accessory information.
Beckman Coulter JS-4.3 rotors are manufactured in a facility that maintains certifications to both
ISO 9001:2008 and ISO 13485:2003. They are for use with the specified Beckman Coulter centrifuges.
See the Warranty at the back of this manual for warranty information.
The Rotor
The JS-4.3 rotor is used in Beckman Coulter Avanti JXN-26, Avanti J-26S XP series, Avanti J-26 XP
series, Avanti J-HC, and in discontinued Avanti J2-HC and Avanti J-20 XP series centrifuges. This
four-place horizontal rotor carries a wide range of tube and bottle sizes (from 1.5 to 750 mL) or
single- to quad-pack blood bags in buckets, as well as 96-well multiwell plates in specially designed
carriers. This rotor rapidly sediments protein precipitates, large particles, cells, and cell debris. It
can also be used for binding studies and for separating serum from whole blood.
The rotor yoke is made of stainless steel. Either buckets or multiwell plate carriers, both made of
anodized aluminum, may be run by placing them over pivot pins on the arms of the yoke. Both
buckets and carriers swing out to a horizontal position during centrifugation.
NOTE Although rotor components and accessories made by other manufacturers may fit in the JS-4.3 rotor,
their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’
components or accessories in these rotors may void the rotor warranty and should be prohibited by your
laboratory safety officer. Only the components and accessories listed in this publication should be used
in these rotors.
Buckets and Accessories
Several types of labware can be placed in the buckets, depending upon your application: modular
disk adapters (for tubes of various sizes), bottle adapters, blood bag cups, and Aerosolve canisters
(when aerosol containment is required). Bucket covers are also available as an aid toward
containment.
Modular Disk Adapters
Tubes are supported in modular disk adapters, which can also serve as tube racks in the laboratory.
The adapter disks are color coded by the tube size they accommodate (see Table 2); the number of
disks used in an adapter assembly depends upon the length of tubes used. A tube decanter is
available to hold either 10-mm or 12-mm tubes securely in the blue adapter, allowing all the tubes
to be decanted at once. Additionally, 1.5-mL Microfuge tubes can be run using a special plate that
fits on top of the blue adapter. Both of these accessories are described in Table 2. Beckman Coulter
tubes and bottles available for use in the JS-4.3 rotor buckets are described in .
PN J-TB-050EB
3
JS-4.3 Swinging-Bucket Rotor
Description
Table 2 Modular Disk Adapters, Bottle Adapters, and Cups Available for the JS-4.3 Rotor
Nom.
Tube
Vol.
(mL)
Color
Code
Nom.
Tube Dia.
(mm)
Max. No.
Tubes
per
Adapter
Max. No.
Tubes in
Rotor
rmax at
Adapter
Bottom
(mm)
RCF at
Maximum
speed (g)
No.
Disks
per
Adapter
Adapter Part No.
Set of
Two
Set of
Four
MODULAR DISK ADAPTERS (polypropylene)
blue
3
5
10
12
37
148
184.7
3830
5
359469
359148
tan
3&5
13
30
120
184.7
3830
5
359478
359157
orange
7 & 10
14
24
96
184.7
3830
6
359470
359149
purple
12
16
19
76
184.7
3830
7
359471
359150
green
(conical)
15
18
14
56
194.7
4030
6
359472
359151
green
15 & 20
18
14
56
184.7
3830
7
359473
359152
lt. green
(conical)
30 & 50
30
4
16
191
3960
5
359475
359154
yellow
50
29
7
28
184.7
3830
6
359474
359153
dk. blue
50
35
4
16
184.7
3830
7
359476
359155
brown
100
44
2
8
184.7
3830
3
359477
359156
tube
decanter
3
5
10
12
37
148
—
—
1
343108a
(each)
—
1.5-mL
adapter
plate
1.5 &
1.8
11
26
104
—
—
1
354511a
(each)
—
a. Tube retainers and adapter plates are sold iindividually.
Color Code
Cup Capacity
Size
(mm)
Number Bags
per Cup
rmax at Cup
Bottom
(mm)
RCF at
Maximum
Speed (´ g)
Part Number
(qty one)
BLOOD BAG CUPS (polypropylene)
4
yellow
single bag double pack
90
2
196.2
4060
356856
orange
triple pack quad pack
97
1
196.7
4040
356857
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Description
Table 2 Modular Disk Adapters, Bottle Adapters, and Cups Available for the JS-4.3 Rotor (continued)
Color
Code
Nom.
Tube
Vol.
(mL)
Nom.
Tube Dia.
(mm)
Max. No.
Tubes
per
Adapter
Max. No.
Tubes in
Rotor
rmax at
Adapter
Bottom
(mm)
RCF at
Maximum
speed
(g)a
Adapter Part No.
Set of
Two
Set of
Four
AEROSOLVE CANISTER RACKS (polypropylene)
white
1.5
11
24
96
174
3600
354495
—
blue
3&5
12
24
96
174
3600
359482
359160
tan
5
13
24
96
180
3730
359489
358993
orange
10
14
18
72
175
3620
359483
359161
purple
12
3&5
16
12
12
6
48
24
177
178
3670
3690
359484
359162
white
(vials)
15
14
10
40
174
3600
344517
—
green
15 & 20
3&5
18
12
12
6
48
24
174
176
3600
3640
359485
359163
lt. green
(conical)
15
3&5
17
12
6
6
24
24
181
180
3750
3730
359487
358991
lime
green
(conical)
50
3&5
30
12
4
4
16
16
181
180
3750
3750
359488
358992
yellow
50
3&5
29
12
4
4
16
16
177
178
3670
3690
359486
359164
orange
230
62
1
4
180
2840
—
356985
Canister
Kitb
500
—
—
—
183
3790
359481
359232
a. Tube racks used with Aerosolve canisters do not provide full tube support; some manufacturers’ plastic and glass tubes cannot withstand
the maximum forces generated by this rotor when used in these racks. Beckman Coulter highly recommends that you pretest other
manufacturers’ tubes (in the appropriate Aerosolve canister labware) using water samples.
b. Canisters and lids are made of polyphenylsulphone; O-rings are ethylene propylene. Canister kit includes the pad that must be used
beneath the canister in the JS-4.3 rotor; sold in sets of two or four.
CAUTION
Do not run chloroformed samples in Aerosolve canisters. Chloroform vapors
can damage the canister material.
PN J-TB-050EB
5
JS-4.3 Swinging-Bucket Rotor
Description
Table 2 Modular Disk Adapters, Bottle Adapters, and Cups Available for the JS-4.3 Rotor (continued)
Color Code
Nominal
Bottle Volume
(mL)
Nominal
Bottle
Diameter
(mm)
Maximum
Number of
Bottles in
Rotor
rmax at Bottle
Bottom
(mm)
RCF at
Maximum
Speed (´ g)
Adapter Part
Number
(qty one)
BOTTLE ADAPTERS (polypropylene)
orange (conical)
230
62
4
195.1
4040
356983
(use with
349946)
yellow
250
62
4
195.1
4040
349946
warm red
(conical)
250
62
4
203.2
4210
349849
lt. purplea
500
70
4
200.2
4150
349945
blue
750
96
4
195.2
4040
349486
a. Light purple adapter replaces the previous tan adapter. See note under Symmetric and Balanced Loading for weight difference information.
Table 3 Beckman Coulter Tubes and Bottles for the JS-4.3 Rotor
Adapter
Dimensions
Volume
(mL)
Description
Part
Number
Set of Two
Set of Four
OPEN-TOP TUBES
16  76 mm
10
polypropylene
355640
359471
359484a
359150
359162a
16  76 mm
10
polycarbonate
355630
359471
359484a
359150
359162a
18  98 mm
15
polycarbonate
342080b
359473
359152
18  98 mm
15
polyethylene
342081b
359473
359152
18  98 mm
15
polypropylene
342082
359473
359152
29  104 mm
50
polycarbonate, graduated
363075
359474
359486a
359153
359164a
29  103 mm
50
polypropylene
357007
359474
359486a
359153
359164a
29  103 mm
50
polycarbonate
363647
359474
359486a
359153
359164a
TUBES WITH SNAP-ON CAPS
6
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Description
Table 3 Beckman Coulter Tubes and Bottles for the JS-4.3 Rotor
Adapter
Dimensions
Volume
(mL)
11  38 mm
1.5
polypropylene
11  38 mm
1.5
11 38 mm
Description
Part
Number
Set of Two
Set of Four
357448
359469
354511c
354495a
359148
polypropylene
356090
359469
354511c
354495a
359148
1.5
blue polypropylene
356091
359469
354511c
354495a
359148
11  38 mm
1.5
green polypropylene
356092
359469
354511c
354495a
359148
11  38 mm
1.5
yellow polypropylene
356093
359469
354511c
354495a
359148
11  38 mm
1.5
orange polypropylene
356094
359469
354511c
354495a
359148
11  38 mm
1.5
polypropylene (cap separate)
343169
359469
354511d
354495a
359148
11  39 mm
1.8
white polyethylene
340196
359469
354511d
354495a
359148
29  103 mm
50
polypropylene
357005
359474
359486a
359153
359164a
29  103 mm
50
polycarbonate
363664
359474
359486a
359153
359164a
17  120 mm
15
polypropylene (graduated)
355663
359472
359487a
359151
358991a
62 141 mm
230
polycarbonate (with cap)
356987
349946c
356983
356985a
62  141 mm
230
polypropylene (with cap)
356989
349946c
356983
356985a
polypropylene
566353
359470
344517a
359149
CONICAL TUBES
BIOVIALS
14  55 mm
PN J-TB-050EB
4
7
JS-4.3 Swinging-Bucket Rotor
Description
Table 3 Beckman Coulter Tubes and Bottles for the JS-4.3 Rotor
Adapter
Volume
(mL)
Description
Part
Number
29  104 mm
50
polycarbonate (with cap assembly)
29  104 mm
50
29  104 mm
Dimensions
Set of Two
Set of Four
361693
59474
359486a
359153
359164a
polycarbonate (with screw cap)
357002
359474
359486a
359153
359164a
50
polypropylene (with cap assembly)
361694
359474
359486a
359153
359164a
29  104 mm
50
polypropylene (with screw cap)
357003
359474
359486a
359153
359164a
62  141 mm
230
conical, wide-mouth polycarbonate
356987e
356983
(use w/ 349946)
—
62  141 mm
230
conical, wide-mouth polypropylene
356989e
356983
(use w/ 349946)
—
62  136 mm
250
polycarbonate (with screw cap,
round bottom)
355673
349946d
—
62  122 mm
250
wide-mouth polycarbonate
(with cap)
356013
349946d
—
62  122 mm
250
wide-mouth polypropylene
(with cap)
356011
349946d
—
62  122 mm
250
wide-mouth polycarbonate
358275
349946d
—
62  120 mm
250
wide-mouth polypropylene
358326
349946d
—
69  160 mm
500
polypropylene (with cap assembly)
355607
349945d
—
69  159 mm
500
polypropylene (with cap)
355665
349945d
—
69  159 mm
500
polypropylene
355650
349945d
—
96  130 mm
750
polycarbonate (with screw capf)
358299c
349846d
—
96  130 mm
750
polypropylene (with screw capf)
356855c
349846d
—
BOTTLES
a. Adapter used in Aerosolve canister (part number 359232).
b. To order caps for 15-mL tubes 342080, 342081, and 342082, use pat number 343656 for a package of 50. Cap 343656 is made of thermoplastic
polyester elastomer. Note that thermoplastic polyester elastomer does not provide the same chemical resistance as the tube materials. Before
using the caps, check with the manufacturer to verify their ability to withstand exposure to the chemical you will be using.
c. Do not load the 750-mL bottle directly into the bucket; always use the adapter.
d. Tube retainers and adapter plates are sold individually.
e. Bucket covers (361262) cannot be used with these tubes.
f. Replacement bottle cap part number is 356263 (set of 6).
8
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Description
Bottle Adapters
Bottles are supported in polypropylene adapters that fit inside the rotor buckets. The adapters are
ribbed for strength and accommodate three bottle sizes, including one conical bottle (see Table 2).
Blood Bag Cups
Polypropylene cups provide support for blood bags in the rotor buckets. Blood bag cups are
available in two sizes: one for single- or double-pack bags, and one for triple- or quad-pack bags
(see Table 2).
Bucket Covers
Transparent covers made of a high-impact plastic are available for the JS-4.3 rotor buckets. Each
cover requires an O-ring (made of ethylene propylene) that seats on a ledge inside the bucket. The
covers are held in place by attached latch assemblies. Although the covers are not designed to contain
aerosols that may result from tube breakage, they will contain most liquids and broken tube
particles, reducing the need to clean the centrifuge chamber, and allowing you to take appropriate
precautions before opening the covers in the event of tube breakage.
Aerosolve Canisters
Aerosolve canisters, designed to contain aerosol leakage and liquid spills, can be used in the JS-4.3
buckets when this additional containment is required. This canister was tested* to demonstrate
containment of microbiological aerosols under normal operating conditions of the associated
Beckman Coulter centrifuge, when used and maintained as instructed. Aerosolve canisters hold a
variety of tube sizes in racks, or they can be used as 500-mL wide-mouth bottles.
Micro Plus Multiwell Plate Carriers
Micro Plus multiwell plate carriers (see Figure 1) are used by installing them on the pivot pins in
place of the buckets normally used with the rotor. Micro Plus carriers are made of aluminum, and
are black-anodized for corrosion resistance. A blue thermoplastic base is used to house the
multiwell plates. A flexible plastic pad between the base and the plate(s) supports the plates during
a run. Each Micro Plus carrier will accommodate a deep-well multiwell plate, or can run up to three
96-well microplates (when more than one are run, one plate is placed directly on top of the other).
Refer to Table 4 for a list of labware that can be used with the carrier. Multiwell plates will break if
g-forces are too high. Rotor speed must not exceed 3250 RPM when Micro Plus carriers are run.
The aluminum carriers are warranted for 7 years; the plastic base and pad are warranted for 1 year
and should be replaced at the end of the warranty period.
* Validation of microbiological containment was done at an independent third-party testing facility (CAMR, Porton Down,
UK, or USAMRIID, Ft. Detrick, MD. U.S.A.). Improper use or maintenance may affect seal integrity and thus containment.
PN J-TB-050EB
9
JS-4.3 Swinging-Bucket Rotor
Description
H
G
F
E
D
C
B
A
Figure 1 The Micro Plus Multiwell Plate Carrier
Table 4 Labware for Use with Micro Plus Carriers
Required Accessory
Description
Multiwell plate, 96-well,
nonsterile
Deep-well polystyrene
plate, 96-well, nonsterile
Deep-well polystyrene
plate, 96-well, sterile
Deep-well polypropylene
plate, 96-well, nonsterile
Deep-well polypropylene
plate, 96-well, sterile
Volume
Part
Number
Description
Part Number
Rack
300 L/well
609844a
—
—
—
Cap strip, nonsterilea
267002
Cap strip, sterilea
267005
Aluminum foil lida
538619
Cap strip, nonsterilea
267002
Cap strip, sterilea
267005
Aluminum foil lida
538619
Cap strip, nonsterilea
267002
Cap strip, sterilea
267005
Aluminum foil lidb
538619
Cap strip, nonsterilea
267002
Cap strip, sterilea
267005
Aluminum foil lidb
538619
1 mL/well
1 mL/well
1 mL/well
mL/well
267001
267004
267006
267007
—
—
—
—
Square-well
polypropylene plate
2 mL/well
140504
Aluminum foil lidb
538619
—
Microfuge tube,
polypropylene, clear
1.5 mL
357448
Rack insert, white
373696
373661
(holds 24 tubes)
Microfuge tube,
polypropylene, clear
1.5 mL
356090
Rack insert, white
373696
373661
(holds 24 tubes)
10
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Installation
Table 4 Labware for Use with Micro Plus Carriers (Continued)
Required Accessory
Description
Volume
Part
Number
Microfuge tube,
polypropylene, blue
1.5 mL
356091
Rack insert, white
373696
373661
(holds 24 tubes)
Microfuge tube,
polypropylene, green
1.5 mL
356092
Rack insert, white
373696
373661
(holds 24 tubes)
Microfuge tube,
polypropylene, yellow
1.5 mL
356093
Rack insert, white
373696
373661
(holds 24 tubes)
Microfuge tube,
polypropylene, orange
1.5 mL
356094
Rack insert, white
373696
373661
(holds 24 tubes)
Description
Part Number
Rack
a. Caps are optional.
b. Requires soft rubber roller (4-in.), part number 538618, for installation.
Installation
The Rotor Yoke
NOTE Before the first use of a new rotor, lubricate the rotor pins and bucket pin sockets as described under
MAINTENANCE. Also, remove the lubrication decal over the rotor drive hole (in the yoke) and lubricate the
inside of the drive hole as described under Inspection.
1
Holding the rotor yoke with both hands, carefully lower it onto the centrifuge drive
spindle hub.
CAUTION
Never drop the yoke onto the spindle hub. The hub can be bent or broken if the
yoke is dropped onto it.
2
Turn the rotor yoke back and forth slightly a few times to make sure that the horizontal pins in
the rotor drive hole are resting on top of the drive spindle hub, next to the hub teeth
(see Figure 2).
Make sure that the pins are not on top of the teeth.
Two pin holes, which are visible on opposite sides of the rotor yoke exterior, indicate location
of the two pins inside the drive hole.
PN J-TB-050EB
11
JS-4.3 Swinging-Bucket Rotor
Installation
Figure 2 Rotor Yoke and Centrifuge Drive Spindle Hub*
Pin Hole
Rotor Drive Hole
Teeth
Centrifuge Drive Spindle Hub
3
Tighten the tie-down knob.
The tie-down knob will not engage with the drive hub if the drive pins are on top of the
hub teeth.
WARNING
Securely tighten the tie-down knob to attach the rotor yoke to the centrifuge drive
spindle hub. If the rotor is left in the centrifuge between runs, make sure the rotor
is seated on the drive spindle and the tie-down knob is tight before each run.
NOTE Do not remove the tie-down knob from the rotor.
Buckets or Carriers
1
Place buckets or carriers over the pivot pins on the rotor yoke.
Fill all four positions on the yoke with buckets and/or carriers.
CAUTION
All four positions on the rotor yoke must contain either a bucket or a carrier during
a run. Never run the rotor with only two positions filled.
2
Make sure buckets or carriers are properly seated by gently swinging them on the pivot pins.
* Be sure the pins in the rotor yoke drive hole are not sitting on top of the teeth on the spindle hub.
12
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Loading the Rotor
You can use four buckets, four carriers, or two buckets and two carriers (with like components
positioned opposite each other). If two buckets and two carriers are used, reduce run speed to the
speed allowed for multiwell plate carriers (refer to Speed Derating, below).
Loading the Rotor
Working with Physiological fluids
WARNING
Handle body fluids with care because they can transmit disease. No known test
offers complete assurance that they are free of micro-organisms. Some of the
most virulent—Hepatitis (B and C) viruses, HIV (I–V), atypical mycobacteria, and
certain systemic fungi —further emphasize the need for aerosol protection.
Take appropriate safety precautions when handling toxic, pathogenic, or other
hazardous materials.
When working with potentially hazardous materials, always fill or open containers in an
appropriate hood or biological safety cabinet. Three levels of containment are offered by
Beckman Coulter, and can be used singly or combined, depending upon your application.
1
Capped tubes or bottles are designed to provide fluid containment.
We strongly recommend that all containers carrying physiological fluids be capped to prevent
leakage.
2
Bucket covers for the JS-4.3 rotor help to contain fluids within the bucket in the event of tube
breakage or blood-bag failure.
3
Aerosolve canisters are designed to minimize the possibility of aerosol (and fluid) leakage
during centrifugation.
Symmetric and Balanced Loading
To ensure optimal performance and stability, the JS-4.3 rotor must be loaded symmetrically (see
Figure 3). Two factors affect symmetric loading:
• The buckets and/or carriers must be loaded symmetrically with respect to their pivotal axes.
• The rotor should be loaded symmetrically with respect to its center of rotation.
PN J-TB-050EB
13
JS-4.3 Swinging-Bucket Rotor
Loading the Rotor
Figure 3 Examples of Correctly and Incorrectly Loaded Buckets and Carriers*
BALANCED LOAD
UNBALANCED LOAD
Pivotal
Axis
of Bucket
Center of
Rotation
CORRECT
INCORRECT
Examples of Symmetrically Loaded Trays
(load opposite trays the same way)
Examples of Nonsymmetrically
Loaded Trays
Pivotal Axis
H
G
F
E
D
Plate
C
B
A
Carrier
* Contents of opposing buckets must be the same and each bucket must be balanced on its pivotal axis.
14
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Loading the Rotor
This means that for best results you should load opposing buckets or carriers with the same type of
labware containing the same amounts of fluid of equal density. Additionally, opposing buckets and
their contents should weigh approximately the same (within 10 grams). Do not exceed the rated
maximum load for either buckets (1000 grams each, including labware and sample) or Micro Plus
carriers (500 grams each, including base, pad, plate(s), and sample).
CAUTION
If only two buckets are loaded with blood bags, bottles, or modular disk adapters,
the other two buckets should contain at least a minimal “blank” load (such as
empty modular disk adapters) to achieve optimal results and to avoid rotor
imbalance. If only two blood bags are being run and modular disk adapters are not
available, we recommend using two water-filled blood bags in cups in the empty
buckets. Placing empty blood bag cups or bottle adapters in the open buckets
does not provide enough weight to prevent possible imbalance.
Beckman Coulter supplies buckets in weight-matched sets to make balancing easier (the weight and
date of manufacture are marked on the side of each bucket). Modular disk adapters are also sold
in weight-matched sets. However, there are variances in weight between sets, as well as variance
in weight between previously purchased adapters. To prevent accidental imbalance it is important
to keep matched sets of adapters together and to weigh other adapters to be sure they are
approximately the same. Marking matched sets will help you keep them together.
NOTE The light purple 500-mL adapters (349945) replace the previous tan adapters. There is a weight
difference between the old tan adapters and the current purple adapters. If you run a mix of the purple
and tan adapters, be sure to run matching colors in opposing positions or rotor imbalance could occur.
It is not necessary to completely fill all tubes, positions in adapters, or wells in multiwell plates;
however, partially filled adapters or multiwell plates must be balanced with respect to the pivotal
axis of the bucket or carrier.
In adapters, each tube should be placed so that its weight is balanced by a tube in a diametrically
opposite position across the pivotal axis in the same adapter (see Figure 3). Adapters placed in
opposing buckets should also be filled the same way.
If you must run only one tube in an adapter, be sure this tube rests over the bucket’s pivotal axis.
• Use the center hole of the adapter, if one is available.
• Use a side hole in line with the bails (vertical supports).
• Turn the adapter so that the tube rests across the pivotal axis of the bucket. The bails will
no longer be in line with the pivotal axis; check that they do not interfere with the proper swing
of the bucket.
NOTE Be sure to run a tube of the same approximate weight in the same configuration in the
opposite bucket.
In multiwell plate carriers, samples should be loaded into the wells symmetrically with respect to
the pivotal axis of the carrier (the pivotal axis runs parallel to the crossbar), and opposing carriers
should contain the same loads (see Figure 3).
PN J-TB-050EB
15
JS-4.3 Swinging-Bucket Rotor
Loading the Rotor
CAUTION
If multiwell plate carriers are not loaded symmetrically with respect to each
carrier’s pivotal axis, the carriers could come off the rotor pivot pins and could
damage the rotor and the centrifuge.
NOTE The same adapters can be used in the JS-4.3 and GH-3.8 rotors (see Table 2). However, do not
intermix adapters (or their individual parts) from Beckman Coulter J6 series rotors with those for the
JS-4.3 rotor. While the adapters are very similar in appearance (see Figure 4), they have very
different weights. J6 adapters have bails that are curved at the top; bails for the JS-4.3 adapters are
straight. Keep J6 and JS-4.3 disks and bases separate—mixing them can cause imbalance.
Figure 4 The Bails (Vertical Supports) of the JS-4.3 (and GH-3.8) Modular Disk Adapters and the J6 Series
Adapters are Not the Same*
Bails
Do not use in the
JS-4.3 rotor.
Bails
J6
JS-4.3
During a run, buckets and carriers swing 90 degrees from their at-rest position. The pivotal axis of
a bucket or carrier can be imagined as a line extending across the bucket or carrier from one pivot
pin to the other. If a bucket or carrier is loaded so that its weight is unequally distributed on either
side of its pivotal axis, it will not hang vertically at rest and, more importantly, may not swing to a
horizontal position during a run (see Figure 5). As a result, extra stress will be placed on the bucket,
carrier, tubes, and/or multiwell plates during the run, increasing the possibility of breakage or rotor
imbalance.
* Bails for the JS-4.3 are straight and end in a disk; bails for the J6 have curved ends. Do not intermix adapters (or disks
from adapters) between sets.
16
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Loading the Rotor
Figure 5 Examples of the Effects on the Horizontal Swing of Buckets Caused
by Proper and Improper Weight Distribution
AT REST
AT SPEED
Proper Weight Distribution
Improper Weight Distribution
Using Buckets
You can load buckets before or after they are installed on the rotor yoke. In either case, we
recommend filling the appropriate labware first and then loading the labware into the buckets. This
is especially important when using blood bags—you can trip the imbalance detector in the
centrifuge by pushing blood bags into cups within buckets already installed in the rotor.
Refer to Table 2 and Table 3 to determine the appropriate labware required for your application.
Whether you are running tubes, bottles, or blood bags, you must load the buckets symmetrically
around the center of rotation and each bucket must be loaded symmetrically with respect to its
pivotal axis (see Symmetric and Balanced Loading, above).
CAUTION
Buckets are designed to be used only with modular disk adapters, bottle adapters,
and blood bag cups. Do not pour samples directly into buckets, bottle sleeves, or
blood bag cups. Do not load bottles or blood bags directly into the buckets. Do not
use labware that is not specified for use in this rotor.
Assembling Modular Disk Adapters
Refer to Table 2 to determine which color adapter is required for the tubes you are using and the
number of disks needed to support the length of the tubes.
1
PN J-TB-050EB
Select the appropriate adapter base (from Table 2) and attach a bail to it (see Figure 6).
17
JS-4.3 Swinging-Bucket Rotor
Loading the Rotor
Figure 6 Assembling a Modular Disk Adapter
Bails
Disks
Base
2
Place the base and bail in an empty bucket or on the lab bench (not in the rotor).
3
Position one of the disks in the bucket so that its grooves are aligned with the bail.
Push the disk down until the bail snaps into the grooves.
4
Add more disks in this way until the height of the assembly is nearly as tall as the tubes you
will be using.
(If the height of the disks is very tall, you may have to push the bail into the grooves of the top
disks by hand.)
Remove or add disks to the bail to accommodate shorter or longer tubes.
If the tubes fit too snugly in the adapter’s rubber base, apply a light film of powder, such as
talcum powder, to prevent the tubes from sticking.
Loading Tubes in Adapters
When placing tubes in modular disk adapters, it is important to make sure that they will not contact
the rotor yoke during a run.
• One way to do this is to place empty tubes in an adapter, place the adapter in a bucket on the
rotor yoke, and manually swing the bucket to the horizontal position, making sure that all tubes
clear the yoke.
• Another method is to place the adapter in a bucket and position a bucket cover over the tubes,
checking that tubes do not touch the cover.
If all positions in an adapter are not filled, the adapter should be loaded symmetrically with respect
to its pivotal axis (see Symmetric and Balanced Loading, above).
18
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Loading the Rotor
Loading Adapters in the Buckets
After placing tubes in the disk adapters, lower the adapters into the buckets so that the bails line up
with the rotor pivot pins (see Figure 7). If only two loaded adapters are run, place them in opposing
buckets. The other two buckets should contain empty adapters to prevent imbalance. (See
Symmetric and Balanced Loading, above.)
Figure 7 Installing an Adapter in a Bucket*
Bails (vertical
supports)
Pivot Pin
Using Bottles in Adapters
Load bottles into their appropriate adapters (see Table 2), then place the filled adapters in the rotor
buckets. If only two bottles are run, place them in opposite buckets. Make sure the other two buckets
contain at least a minimal “blank” load (such as empty modular disk adapters) to prevent rotor
imbalance. If modular disk adapters are not available, use two water-filled bottles in adapters to
balance the load. (See Symmetric and Balanced Loading, above.)
Using Blood Cups
NOTE This rotor is suitable for obtaining cell-free plasma or for cell packing, not for separating blood
components.
The yellow 90-mm cup (356856) accommodates up to two single bags or one double pack; the orange
97-mm cup (356857) supports either one triple or one quad pack. Do not pour liquid directly into blood
bag cups. Fit blood bags into cups before loading the cups into the rotor buckets—you can trip the
imbalance detector in the centrifuge by pushing blood bags into cups within buckets installed in the
rotor. Load the blood bag cups as follows:
* Be sure the bails (vertical supports) line up with the bucket pivot pins.
PN J-TB-050EB
19
JS-4.3 Swinging-Bucket Rotor
Loading the Rotor
1
Load the bags as far down into the cups as possible.
NOTE Make sure the bags stay as vertical as possible, with no folds at the top or corners. If folds are
present, blood cells could remain in the folds and then mix with the plasma when the bag is removed.
2
Sandwich the tubing between the blood bag and any transfer packs (see Figure 8).
3
Make sure the loaded blood bag cups opposite each other on the rotor yoke are approximately
the same weight (within 1 gram).
Soft pliable balancing pads that will not puncture blood bags (see the Supply List) can be used if
necessary to maintain weight balance.
4
Place loaded cups into rotor buckets. To reduce the possibility of bag breakage, align the blood
bag seam with the rotor pivot pins (see Figure 8) with the label facing out.
5
Place bucket covers on rotor buckets to prevent blood bags or tubing from contacting the rotor
yoke during centrifugation.
CAUTION
If bucket covers are not used, make sure the superstructure of the blood bag
protruding from the cup does not inhibit the bucket from reaching its horizontal
position. If it does, remove the cup from the rotor and reposition the blood bag so
that it seats further into the cup. Allowing the blood bags to contact the rotor yoke
during centrifugation can cause the bucket to come off the pivot pins and can
seriously damage both the rotor and the centrifuge.
If only two filled cups are run, place them in opposing buckets. The other two buckets should
contain similar “blank” loads to prevent imbalance (either empty modular disk adapters or waterfilled blood bags in cups).
Bucket Covers
Beckman Coulter recommends that you use bucket covers (361262) to help contain spills and glass
particles in the event of tube breakage. Bucket covers can be used with all blood bags, bottles (except
230-mL conical), and tubes up to 130 mm long. They are not designed to contain aerosols.
NOTE Because of the shape of the covers, 130-mm tubes can be used only in the center cavities of the
adapters when covers are used.
20
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Loading the Rotor
Figure 8 Blood Bag Loading Procedures
Blood Bag Label
Blood Bag Seam
Aligned with
Pivotal Axis of
Bucket
Sandwich Coiled
Tubing between
Transfer Bags
and Filled
Blood Bag
Blood Bag Label
Ports
Blood
Bag
Seam
Blood
Bag
Label
Blood Bag
Label
Blood Bag Label
Blood Bag Cup
Install the cover as follows:
1
Make sure that the bucket and the bucket cover surface are clean, dry, and undamaged.
2
Make sure that the O-ring (961648) is in good condition and lightly coated with silicone vacuum
grease (335148).
3
Place the O-ring on the ledge inside the bucket (see Figure 9).
Figure 9 Proper Placement of O-ring
Insert
O-ring here
PN J-TB-050EB
21
JS-4.3 Swinging-Bucket Rotor
Loading the Rotor
4
Place the cover on the bucket with the latches perpendicular to the pin sockets (see Figure 10).
Figure 10 Securing Bucket Cover Latches
Vent Plug
Cover
Spring
Snap Down
Latch
Bucket
Pin Socket
5
Snap the latches down and secure them under the bucket rim.
6
Push the vent plug down to seal the cover.
NOTE To remove the cover after a run, gently lift one side of the vent plug to release the interior vacuum.
Carefully release the latches, then remove the cover.
Using Aerosolve Canisters
CAUTION
Do not run chloroformed samples in Aerosolve canisters. Chloroform vapors can
damage the canister material.
Aerosolve canisters can be used as wide-mouth bottles or with tubes in racks that are specially
designed to fit in the canisters. Table 2 lists the Aerosolve tube racks and the number and sizes of
tubes they accommodate. When Aerosolve canisters are used in the JS-4.3 rotor buckets, pads
(361269) must be placed beneath the canisters in the buckets to provide support and prevent the
canisters from being damaged during centrifugation. These pads are included in the JS-4.3
Aerosolve canister kits (359232).
22
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Loading the Rotor
CAUTION
Be sure to remove the pad when you remove the canister; placing other
accessories on top of the pad could cause tube breakage, rotor imbalance,
improper run results, or rotor mishap. Tubes, bottles, or blood bags placed in
labware that rests on Aerosolve canister pads will be closer to the rotor yoke and
can interfere with the proper swing of the bucket.
Always remove the bucket cover O-ring (see Figure 9) from the bucket before using an Aerosolve canister.
If this O-ring is not removed, centrifuge chamber vacuum may make it difficult to remove the canister from
the buckets.
If using tube racks, load them symmetrically (refer to Figure 3). If only two canisters are being run,
place the canisters in opposite buckets; place empty adapters (or “blank” loads) in the two empty
buckets for balance.
Read publication TJ6-TB-011, supplied with the canister, for complete information regarding its use
and maintenance.
Using Micro Plus Multiwell Plate Carriers
Micro Plus plate carriers (see Figure 11) can be used in place of the buckets normally used with the
rotor. (For complete information about the carriers, see publication GS6-TB-011, which
accompanies the carriers.) If only two carriers are run, they must be installed opposite each other
in the rotor, and either two buckets or two other carriers must also be run (they need not be loaded)
to prevent rotor imbalance. The rotor should never be run with only two buckets or two carriers installed.
Opposing Micro Plus carriers must balance to within 10 grams. (See Symmetric and Balanced Loading
above.)
NOTE If symmetrical pelleting is required, use round (“U-bottom”) or “V-bottom” multiwell plates
(commercially available from many suppliers) for best results.
Figure 11 The Micro Plus Multiwell Plate Carrier, Base, Pad, and Deep-well Plate
Deep-well Plate
H
G
F
E
D
C
B
A
Carrier
Base
Pad
PN J-TB-050EB
23
JS-4.3 Swinging-Bucket Rotor
Operation
Load a multiwell plate into a carrier as follows.
1
To prevent plate breakage during centrifugation, place the flexible plastic pad (362390), ridged
side up, into the flat, indented area of the blue base (361302).
2
Place the filled labware on top of the pad, being careful not to spill the contents.
If you are using two or three microplates, place a 96-well cap strip (267002) between the plates
to prevent plate breakage during centrifugation.
3
Slide the base, pad, and plate assembly into the carrier until the base locating feet click
into place.
IF YOU USE MORE THAN ONE JS-4.3 ROTOR
Complete sets of buckets, Micro Plus plate carriers, and labware may be used in any JS-4.3 rotor.
Remember the following points:
• Buckets are sold in weight-matched sets. Keep matched sets together and check their weights
prior to use to avoid centrifuge imbalance.
• Modular disk adapters and tube racks for Aerosolve canisters are sold in weight-matched
sets. Mark sets of adapters so that they can be easily identified. When cleaning adapters,
do not mix sets. Check the weight of adapters prior to use.
• Do NOT intermix adapters (or their components) from Beckman Coulter’s J6 series rotors
with those used in the JS-4.3 rotor. These adapters are similar in appearance (see Figure 4) but
have very different weights and functional characteristics. Mixing them will cause centrifuge
imbalance and potential instrument damage.
Operation
Speed Derating
The maximum allowable run speed (4300 RPM) listed in the rotor specifications is for operation
when all conditions are within the standard specifications (using four buckets). Maximum allowable
run speed for the JS-4.3 rotor must be reduced in any of the following circumstances.
• Maximum allowable run speed when Micro Plus plate carriers are used is 3250 RPM. If two
buckets and two carriers are used, reduce run speed to 3250 RPM.
• If the weight of the load in a bucket exceeds 1000 grams, or if the solution density is greater than
1.2 g/mL, use the following square-root reduction formula to determine the allowable rotor
speed:
speed = (4300 RPM)
24
1000 grams
-----------------------------------------------------heaviest load in grams
EQ 1
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Operation
• If the weight of the load in a Micro Plus multiwell plate carrier exceeds 500 grams, including base,
pad, labware, and sample, use the following square-root reduction formula to determine the
allowable rotor speed:
speed = (3250 RPM)
EQ 2
500 grams
-----------------------------------------------------heaviest load in grams
• When certain plastic tubes made by other manufacturers are used in Aerosolve canister tube
racks, you must reduce speed to prevent tube deformation or breakage, as these racks do not
provide full tube support. Beckman Coulter recommends that you pretest other manufacturers’
tubes in these racks using water samples to determine run speed and duration reductions to
avoid tube deformation or breakage.
• When using certain glass tubes, you must reduce rotor speed to prevent tube breakage. Table 5
indicates recommended g-force limits to minimize breakage and is intended as a guide only.
Because the strength of glass and plastic tubes can vary between lots, and will depend on
handling and usage, Beckman Coulter highly recommends that you pretest these tubes in the
appropriate labware in the JS-4.3 rotor using water samples to determine optimal operating
conditions.
NOTE Scratches (even microscopic ones) significantly weaken glass tubes.
Table 5 Recommended g-Force Limits to Minimize Breakage of Certain Glass Tubes and Bottles Made by
Other Manufacturers in the JS-4.3 Rotor
PN J-TB-050EB
Tube Description
Volume
(mL)
Dimension
s (mm)
Safe
g Force
(RCF)
Safe
Speed
(RPM)
Modular
Disk Adapter
Color
Pyrex, Kimax, B-D, or
Dispo (RB or FB)
3
or less
10  75
max
3750
blue
Pyrex (LT)
3
11 75
max
3750
blue
B-D Vac (RB, plain)
5a
12  75
max
3750
blue
Kimax (RB)
5
12  75
max
3750
blue
Pyrex 9820 (RB)
5a
12  75
max
3750
blue
B-D SST (RB, SB)
7a
13  100
max
3750
orange
B-D Vac (RB, plain)
7a
13  100
max
3750
orange
B-D SST (RB, SB)
10a
16  100
3000
3600
purple
B-D SST (RB, plain)
10a
16  100
max
3750
purple
Kimax 45049 (RB)
10
16  100
max
3750
purple
Kimax 45200 (LT, G, SC)
10
17  116
max
3750
purple
Pyrex 9820 (RB)
10
16  100
max
3750
purple
Pyrex 8140 (LT, G)
12
17  117
max
3750
purple
25
JS-4.3 Swinging-Bucket Rotor
Operation
Table 5 Recommended g-Force Limits to Minimize Breakage of Certain Glass Tubes and Bottles Made by
Other Manufacturers in the JS-4.3 Rotor (Continued)
Tube Description
Volume
(mL)
Dimension
s (mm)
Safe
g Force
(RCF)
Safe
Speed
(RPM)
Modular
Disk Adapter
Color
B-D SST (RB, SB)
15a
16  130
2700
3500
green
B-D Vac (RB, plain)
15a
16  130
2900
3550
green
Corex 8060A (LT)
15
17  120
max
3750
green
Corex 8080A (LT, G)
15a
17  120
max
3750
green
Corex 8442 (RB)
15
16  115
max
3750
green
Corvac (RB, SB)
15a
16  131
max
3750
green
Kimax (LT, G)
15
17  118
3000
3600
green
Pyrex 8500 (ST)
15
17  110
2700
3500
green
Pyrex 8080 (LT, G)
15a
17  120
max
3750
green
Pyrex 8320 (ST)
40
29  116
max
3750
yellow
Kimax 45212 (ST)
50a
29  118
1000
2100
yellow
Pyrex 8280 (ST)
50a
29  115
2500
3300
yellow
a. Most common usage.
Code:
B-D = Becton DickinsonLT = long taperST = short taper
Dispo = disposableRB = round bottomVac = Vacutainer (B-D)
FB = flat bottomSB = silicone gel barrier
G = graduatedSC = screw capped
Temperature Limits
• Beckman Coulter plastic containers have been centrifuge tested for use at temperaturs between
2 and 25 C. For centrifugation at other temperatures, pretest containers under anticipated run
conditions.
• If plastic containers are frozen before use, make sure that they are thawed to at least 2 C before
centrifugation.
Run Procedure
1
When the rotor is properly loaded and installed in the centrifuge, you are ready to perform
the run.
Refer to the appropriate centrifuge instruction manual for operating instructions.
2
26
Precool the rotor in the centrifuge or in a refrigerator before use—especially before short
runs—to ensure that the rotor reaches the set temperature.
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Operation
A suggested precooling cycle is a minimum of 30 minutes at 2000 RPM at the required
temperature.
3
If fluid containment required, use capped bottles or tubes. It is strongly recommended that all
containers carrying physiological fluids be capped, and not overfilled, to prevent leakage.
WARNING
Operator error or tube failure may generate aerosols. Toxic, pathogenic, or other
hazardous materials must not be run in this rotor unless all appropriate safety
precautions are taken. Also see Working with Physiological fluids, above.
CAUTION
Make sure that the rotor is properly seated on and securely fastened to the drive
hub before each run.
NOTE Do not use the maximum brake setting when microplate carriers are run, as the force of the
deceleration will disturb the contents of the plate(s). For Avanti J series centrifuges, use the SLOW decel
setting. For the J2-HC centrifuge, set the brake selector knob to 0 (no brake).
Temperature Control
To ensure that the JS-4.3 rotor reaches the required temperature during the run, follow the
appropriate instructions below for the J centrifuge model being used.
Avanti J Series Centrifuges
Enter the run temperature according to the instructions in your centrifuge instruction manual.
No additional input is required.
J2-HC Centrifuge (Discontinued)
Enter the required run temperature and the appropriate temperature compensation settings (see
Table 6) on the control panel following the instructions below.
PN J-TB-050EB
1
Enter the required rotor temperature using the centrifuge SET knob.
2
Enter the temperature compensation units specified in Table 6, using the centrifuge
COMP knob.
27
JS-4.3 Swinging-Bucket Rotor
Care and Maintenance
Table 6 J2-HC Centrifuge Temperature Compensation Settings for the JS-4.3 Rotor
Rotor
Speed
(RPM)
Required Sample Temperature (°C, green bar)
–20°
–10°
2°
5°
10°
20°
40°
4300
0
0
0
0
0
0
+2
3000
0
0
0
0
0
0
+2
1500
0
0
0
0
0
0
Na
a. “N” indicates that the rotor cannot achieve the required temperature at this speed.
At the End of the Run
CAUTION
If disassembly reveals evidence of leakage, you should assume that some fluid
escaped the rotor. Apply appropriate decontamination procedures to the
centrifuge, rotor, and accessories.
1
When the run is finished, remove the buckets or carriers from the rotor yoke and set them on a
bench or table.
2
If you used buckets with covers, gently pull up on one side of the black rubber vent plug in each
cover to release the vacuum inside the buckets before attempting to remove the covers.
Care and Maintenance
NOTE Accumulations of dust or other foreign matter can damage rotor pins (resulting in rotor vibrations). To
prevent this, clean the centrifuge bowl daily. Spray the centrifuge bowl with an antistatic solution and
wipe it clean. (Antistatic wipes are also available.)
Inspection
1
Periodically (at least monthly) inspect the rotor yoke, buckets, and/or multiwell plate carriers,
especially inside cavities, for rough spots or pitting, white powder deposits —frequently
aluminum oxide—or heavy discoloration.
If any of these signs are evident, do not run the rotor.
Contact your Beckman Coulter representative for information about the Field Rotor Inspection
Program and the rotor repair center.
28
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Care and Maintenance
2
Before using the rotor, inspect the rotor drive pins to ensure that they are not damaged.
Damaged drive pins can prevent the rotor from seating properly on the centrifuge drive hub
(Avanti J series centrifuges only).
To inspect the drive pins, turn the rotor upside down and look into the drive hole in the center
of the rotor.
If the drive pins appear damaged, contact Beckman Coulter Field Service.
3
Regularly check the condition of the Micro Plus plate carrier base (361302) and pad (362390) and
do not use them if there are visible signs of damage.
Maintenance
Buckets and Micro Plus carriers are made of anodized aluminum. Do not use sharp tools on them, as
scratches in the anodized surface may lead to corrosion.
1
Approximately every three months (or every 250 runs) and after cleaning and/or autoclaving,
wipe the rotor pins and pin sockets (see Figure 12) with a paper towel, then coat with Paint On
Graphite Lubricant (977212).
Allow the lubricant to dry for at least 5 minutes before installing the rotor in a centrifuge.
Figure 12 Lubricating Pivot Pin/Bucket Contact Areas
Lubrication Points
(all pins)
Lubrication Area
(both pin sockets)
2
PN J-TB-050EB
Before first use of the rotor, and after washing, apply Anti-Seize (961660) to the rotor yoke drive
hole as described under Cleaning, below.
29
JS-4.3 Swinging-Bucket Rotor
Care and Maintenance
3
Refer to publication TJ6-TB-011 for information on Aerosolve canister maintenance.
4
Regularly check the condition of the Micro Plus base and rubber pad and do not use them if
there is visible damage.
Refer to Chemical Resistances (publication IN-175) for the chemical compatibilities of rotor and tube
materials. Your Beckman Coulter representative provides contact with the Field Rotor Inspection
Program and the rotor repair center.
Cleaning
NOTE Do not wash the rotor components or accessories in a dishwasher. Do not soak components in
detergent solution for long periods such as overnight.
The Rotor Components
1
If spillage has occurred, or if salt solutions or other corrosive materials are used, clean the rotor
and components immediately.
Do not allow corrosive materials to dry on rotor components.
2
Approximately once a week clean the pins and bucket pin sockets, then lubricate as described
under Maintenance
3
Under normal conditions, remove the rotor from the centrifuge at least once a month for
cleaning and lubrication to prevent buildup of residues.
4
Use a mild detergent such as Solution 555 (339555) and a soft brush to clean the rotor yoke and
tie-down knob.
Dilute the detergent with water (10 parts water to 1 part detergent).
The Rotor Cleaning Kit (339558) contains two quarts of Solution 555 and brushes that will not
scratch the rotor.
5
Thoroughly rinse the cleaned rotor and components with water and air-dry upside down.
Do not use acetone to dry the rotor.
When the rotor is dry, lubricate the bucket sockets and pins as described under Maintenance.
Before reinstalling the rotor, lightly lubricate the drive hole of the rotor with Anti-Seize
(961660) to prevent the rotor from sticking, as follows:
a. Apply the lubricant onto a swab.
30
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Care and Maintenance
b. Draw the coated swab through a paper towel to remove excess lubricant.
c. Lightly coat the inside of the drive hole with the lubricant remaining on the swab.
Buckets
1
Remove the O-ring from the bucket before cleaning.
2
Clean the bucket and O-ring with a mild detergent such as Solution 555, diluted with water
(10 parts water to 1 part detrgent), then rinse thoroughly and dry completely.
3
Coat the O-ring with a light film of silicone vacuum grease (335148).
Place the O-ring on the ledge inside the bucket.
4
After cleaning, lubricate bucket pin sockets with Paint On Graphite Lubricant (977212) as
described under Maintenance.
Micro Plus Plate Carriers (Including Base and Pad), Blood Bag Cups,
and Bucket Covers
Wash with a mild detergent such as Solution 555, diluted with water (10 parts water to 1 part
detergent), and a soft brush. Thoroughly rinse them and air-dry upside down.
Modular Disk Adapters
1
To disassemble adapters for washing, first pull the bail out of the groove in the disks, then
remove the disks and unsnap the bail from the rubber bottom.
2
Use a mild detergent such as Solution 555, diluted with water (10 parts water to 1 part
detergent), and a soft brush to scrub the adapters.
3
Rinse and dry, then reassemble.
4
If necessary, apply a light film of silicone vacuum grease to the grooves on the rubber bottom to
make reassembling easier.
Keep matched sets of adapters together; do not intermix sets.
PN J-TB-050EB
31
JS-4.3 Swinging-Bucket Rotor
Decontamination
Aerosolve Canisters
Refer to publication TJ6-TB-011.
Decontamination
If the anodized aluminum buckets or carriers become contaminated with radioactive material,
decontaminate them using a solution that will not damage their anodized surfaces.
Beckman Coulter has tested a number of solutions and found two that do not harm anodized
aluminum: RadCon Surface Spray or IsoClean Solution (for soaking),* and Radiacwash.†
NOTE IsoClean can cause fading of colored anodized surfaces. Use it only when necessary and remove it
promptly from surfaces.
Beckman Coulter does not however, warrant the performance of these products with respect to
their effect on the carriers or their ability to decontaminate these parts. Consult your laboratory
safety officer regarding the proper decontamination methods to use.
If the rotor or other components are contaminated with toxic or pathogenic materials, follow
appropriate decontamination procedures as outlined by your laboratory safety officer.
Sterilization and Disinfection
• The rotor yoke, buckets, tube adapters, and aluminum parts of multiwell plate carriers can be
autoclaved at 121°C for up to an hour. Blood bag cups, bucket covers, and Aerosolve canisters
purchased after April 1993 (see the date code on the canister bottom) can be autoclaved at that
temperature for about 15 minutes. Do not autoclave the blue plastic Micro Plus carrier base or
Aerosolve canisters purchased prior to April 1993.
• Ethanol (70%) may be used on all rotor components, including those made of plastic. Refer to
Chemical Resistances (publication IN-175) for other chemical resistances of tubes, bottles, and
accessories.
CAUTION
Ethanol is a flammability hazard. Do not use it in or near operating centrifuges.
While Beckman Coulter has tested these methods and found that they do not damage components,
no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection
is a concern, consult your laboratory safety officer regarding proper methods to use.
* In U.S., contact Nuclear Associates (New York); in Eastern Europe and Commonweath States, contact Victoreen GmbH
(Munich); in South Pacific, contact Gammasonics Pty. Ltd. (Australia); in Japan, contact Toyo Medic Co. Ltd. (Tokyo).
† In U.S., contact Biodex Medical Systems (Shirley, New York); internationally, contact the U.S. office to find the dealer
closest to you.
32
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Decontamination
Tube Breakage
CAUTION
To reduce the potential for corrosion, clean buckets or carriers thoroughly
immediately following a tube or well plate breakage.
If a glass tube breaks, remove the glass very carefully from the adapter and bucket. Imbedded glass
particles that remain in the bucket or adapters can cause tube failure during subsequent runs.
Aerosolve Canisters
WARNING
When working with potentially hazardous materials, open canisters in an
appropriate hood or biological safety cabinet in case of tube breakage.
If a glass tube breaks in an Aerosolve canister tube rack, discard and replace the O-ring and rubber
tube cushion in the base of the rack. Imbedded glass particles that remain in the bucket or adapters
can break tubes during subsequent runs.
SST or Corvac Tubes
If an SST or Corvac tube breaks, the tube’s silicone gel barrier material becomes laced with glass
fragments and the tube contents contaminate the entire tube adapter and bucket (or tube rack and
canister).
If one of these tubes breaks in an adapter in a bucket, use the following procedure.
1
Fill the bucket with water.
2
Autoclave the entire bucket and contents at 121°C for 20 minutes.
3
Decant any remaining water and dislodge the tube adapter (do not remove it) over a waste
container.
4
Working under a hood and using plastic gloves, immerse the bucket and tube adapter (intact)
in a 2-liter beaker or similar vessel containing ethyl acetate
CAUTION
Ethyl acetate is a flammability hazard. Do not use it near operating centrifuges.
PN J-TB-050EB
33
JS-4.3 Swinging-Bucket Rotor
Decontamination
5
Scrub the adapter with a brush and decant the ethyl acetate mixture into a waste bottle for
proper disposal.
6
Rinse with water and air-dry the bucket and tube adapter.
If one of these tubes breaks in an Aerosolve canister, use the following procedure.
34
1
Working in a biologic safety cabinet and wearing plastic gloves, open the canister and immerse
the canister, lid, and contents (intact) in a 2-liter beaker or similar vessel containing 5% sodium
hypochlorite (undiluted bleach).
2
Leave the canister to soak overnight.
3
Carefully dislodge the tube rack and scrub it with a brush.
4
Decant the bleach into a waste bottle for proper disposal.
5
Rinse with water and air-dry the canister and tube rack.
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Returning a Rotor
Returning a Rotor
Before returning a rotor or accessory for any reason, prior permission must be obtained from
Beckman Coulter, Inc. A return form may be obtained from your local Beckman Coulter sales office.
The form should contain the following information:
• rotor type and serial number,
• history of use (approximate frequency of use),
• reason for the return,
• original purchase order number, billing number, and shipping number, if possible,
• name and email address of the person to be notified upon receipt of the rotor or accessory at
the factory,
• name and email address of the person to be notified about repair costs, etc.
To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from
pathogens and/or radioactivity. Sterilization and decontamination must be done before returning
the parts. Smaller items (such as tubes, bottles, etc.) should be enclosed in a sealed plastic bag.
All parts must be accompanied by a note, plainly visible on the outside of the box or bag, stating that they
are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach
this notification will result in return or disposal of the items without review of the reported problem.
Use the address label printed on the return form when mailing the rotor and/or accessories.
Customers located outside the United States should contact their local Beckman Coulter office.
PN J-TB-050EB
35
JS-4.3 Swinging-Bucket Rotor
Supply List
Supply List
NOTE Publications referenced in this manual can be obtained at www.beckmancoulter.com, by
calling Beckman Coulter at 1-800-742-2345 in the United States, or by contacting your local
Beckman Coulter office.
See the Beckman Coulter High Performance, High Speed, High Capacity Rotors Tubes & Accessories
catalog (BR-8102, available at www.beckmancoulter.com) for detailed information on ordering
parts and supplies. For your convenience, a partial list is given below.
Replacement Rotor Parts
Description
Part Number
Rotor assembly (includes rotor yoke and 4 buckets)
362734
Anodized aluminum buckets (set of 4)
361261
Bucket cover kit (set of 2) each set includes:
cover assembly
vent plug
O-ring (ethylene-propylene)
silicone vacuum grease (2 oz)
361264
361262
343685
961648
335148
Micro Plus multiwell plate carriers, includes bases
and pads (set of 2)
362394
Micro Plus replacement carriers w/o bases and
pads (set of 2)
361304
Replacement bases for Micro Plus carrier, includes
bases and pads (set of 2)
361302
Replacement pads for Micro Plus carrier (set of 4)
362390
Rotor tie-down knob assembly
362737
Supplies
NOTE For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com.
Description
36
Part Number
Modular disk adapters (polypropylene)
see Table 2
Tubes and bottles
see Table 3
Micro Plus labware
see Table 4
Soft rubber roller (for aluminum foil lids)
538618
Tube decanter (for use with blue adapter) includes:
gasket for 10-mm dia tubes (silicone)
gasket for 12-mm dia tubes (silicone)
343108
343106
343107
PN J-TB-050EB
JS-4.3 Swinging-Bucket Rotor
Supply List
Description
Adapter plate,1.5-mL (for use with blue adapter)
354511
Aerosolve canister kit (set of 4) (each kit includes 1 canister, 1 1id,
1 O-ring, silicone vacuum grease, and 1 pad)
359232
Aerosolve canister O-ring (ethylene-propylene)
345366
Aerosolve pad (placed beneath canister in bucket)
361269
Tube racks for Aerosolve canisters
PN J-TB-050EB
Part Number
see Table 2
Neoprene tube cushions
for blue rack (pkg of 24)
for orange rack (pkg of 18)
for purple rack (pkg of 18; 12 large, 6 small)
for dark green rack (pkg of 18; 12 large, 6 small)
or yellow rack (pkg of 8; 4 large, 4 small)
344117
344118
344119
344120
344121
Blood bag cup, 90-mm, yellow (polypropylene)
356856
Blood bag cup, 97-mm, orange (polypropylene)
Balancing pads (six pads, 3 grams each, red)
Balancing pads (six pads, 6 grams each, grey)
356857
358365
358364
Microfuge tube rack inserts:
Blue insert, 13-mm dia (25/pkg)
Red insert, 12-mm dia (25/pkg)
White insert, 11-mm dia for 1.5-mL Microfuge tubes (25/pkg)
Black insert, 10-mm dia (25/pkg)
373697
373698
373696
373699
Rotor Cleaning Kit
339558
Solution 555 (1 qt)
339555
Silicone vacuum grease (1 oz)
335148
Anti-Seize (1 1/2 oz)
961660
Paint On Graphite Lubricant
977212
37
JS-4.3 Swinging-Bucket Rotor
Supply List
38
PN J-TB-050EB
Beckman Coulter, Inc.
J Series Swinging-Bucket Rotor Warranty
Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc.,
terms and conditions of sale in effect at the time of sale, Beckman Coulter, Inc. agrees to correct
either by repair, or, at its election, by replacement, any defects of material or workmanship
which develop within seven (7) years after delivery of a J series rotor to the original buyer by
Beckman Coulter, Inc. or by an authorized representative, provided that investigation and factory
inspection by Beckman Coulter discloses that such defect developed under normal and proper use.
Should a Beckman Coulter centrifuge be damaged due to a failure of a rotor covered by this
warranty, Beckman Coulter will supply free of charge all centrifuge parts required for repair.
Replacement
Any product claimed to be defective must, if requested by Beckman Coulter be returned to the
factory, transportation charges prepaid, and will be returned to Buyer with the transportation
charges collect unless the product is found to be defective, in which case Beckman Coulter will pay
all transportation charges.
A defective rotor will be replaced by Beckman Coulter at its then current list price less a credit based
upon the age of the rotor (years since date of purchase). The Buyer shall not receive credit until the
claimed defective rotor is returned to Beckman Coulter’s Indianapolis, Indiana facility or delivered
to a Beckman Coulter Field Service representative.
The replacement price (cost to Buyer) for the respective rotor shall be calculated as follows:
years
7
Replacement price = Current rotor list price × ------------Conditions
1. Except as otherwise specifically provided herein, this warranty covers the rotor only and
Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including
but not limited to (i) tubes, (ii) tube caps, (iii) tube adapters, or (iv) tube contents.
2. This warranty is void if the rotor has been subjected to customer misuse such as operation or
maintenance contrary to the instructions in the Beckman Coulter rotor or centrifuge manual.
3. This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge
unmatched to the rotor characteristics, or is operated in a Beckman Coulter centrifuge that has
been improperly disassembled, repaired, or modified.
Disclaimer
IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES
OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT BECKMAN COULTER,
INC. SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND
WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR,
MAINTENANCE, OR REPLACEMENT OF THE PRODUCT.
PN J-TB-050EB
Warranty-1
Related Documents
Rotors and Tubes for
Beckman Coulter J2, J6,and
Avanti J Series Centrifuges
PN JR-IM-10
• Rotors
• Tubes, Bottles, and
Accessories
• Using Tubes and
Accessories
• Using Fixed-Angle Rotors
• Using Swinging-Bucket
Rotors
• Using Vertical-Tube and
Rack Type Rotors
• Care and Maintenance
• Chemical Resistances
• Temperature
Compensation Tables
• Gradient Materials
• Blood Component
Separation
Available in electronic pdf or
CD-ROM by request.
Avanti J-26S XP
PN B10087
Avanti J-26S XPI
PN B10093
Avanti J-26 XP
PN J326XP-IM-5
Avanti J-26 XPI
PN J326XPI-IM-4
Avanti J-HC
PN J3HC-IM-5
Avanti JXN
PN B38322
PN B38323
Aerosolve Canisters
PN TJ6-TB-011
Using Micro Plate Multiwell
Carriers
PN GS6-TB-011
Chemical Resistances for
Beckman Coulter
Centrifugation Products
PN IN-175
High Performance, High
Speed, High Capacaity
Rotors, Tubes, & Accessories
PN BR-1802
Available in hard copy by
request.
Available at
www.beckmancoulter.com
www.beckmancoulter.com
© 2016 Beckman Coulter, Inc.
All Rights Reserved
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