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ECONOMY WATER-JACKETED CO
2
INCUBATOR
110 - 120 Voltage
Installation and
Operation Manual
SCO6WE
Previously Designated:
2406
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SCO6WE WATER-JACKETED CO
2
INCUBATOR
Installation and Operation Manual
Part Number (Manual): 4861748
Revision: November 7, 2017
These units are TÜV CUE listed as water jacket incubators for professional, industrial, or educational use where the preparation or testing of materials is done at an ambient air pressure range of 22.14 – 31.3 inHg (75 – 106 kPa) and no flammable, volatile, or combustible materials are being heated.
These units have been tested to the following requirements:
CAN/CSA C22.2 No. 61010-1:2012
CAN/CSA C22.2 No. 61010-2-010:2004 Reaffirmed: 2014-07
UL 61010-1:2012-05
UL 61010A-2-010:2002-03
EN 61010-1:2010
EN 61010-2-010:2014
Supplemented by: UL 61010-2-010:2015
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TABLE OF CONTENTS
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INTRODUCTION
Thank you for purchasing a Shel Lab product. We know that in today’s competitive marketplace customers have many choices when it comes to constant temperature equipment. We appreciate you choosing ours. Our continued reputation as a leading laboratory product manufacturer rests with you. We stand behind our products and will be here for you if you need us.
These incubators are intended for laboratory, industrial, and educational microbiological cultivation applications. These incubators are not intended for use in hazardous or household locations.
Before using the unit, read this manual in its entirety to understand how to install, operate, and maintain the incubator in a safe manner. Keep this manual available for use by all operators.
Ensure that all operators are given appropriate training before the incubator begins service.
G
ENERAL
S
AFETY
C
ONSIDERATIONS
Note: Failure to follow the guidelines and instructions in this manual may create a protection impairment by disabling or interfering with the unit safety features. This can result in injury or death.
Your unit and its recommended accessories are designed and tested to meet strict safety requirements. It is designed to connect to a power source using the specific power cord type shipped with the unit.
For continued safe operation of your unit, always follow basic safety precautions including:
• Always plug the unit power cord into a protective earth grounded electrical receptacle
(outlet) that conforms to national and local electrical codes. If the unit is not grounded properly, parts such as knobs and controls can conduct electricity and cause serious injury.
•
•
Do not bend the power cord excessively, step on it, or place heavy objects on it.
A damaged cord can be a shock or fire hazard. Never use a power cord if it is damaged or altered in any way.
• Always position the unit so that end-users can quickly unplug it in the event of an emergency.
•
•
•
Do not attempt to move the unit while in operation or before the unit has cooled.
Do not stack the unit without a factory-approved stacking rack or adaptor.
Use only approved accessories. Do not modify system components. Any alterations or modifications to your incubator can be dangerous and void your warranty.
• Follow all local ordinances in your area regarding the use of this unit. If you have any questions about local requirements, please contact the appropriate agencies.
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INTRODUCTION (CONTINUED)
E
NGINEERING
I
MPROVEMENTS
Sheldon Manufacturing continually improves all of its products. As a result, engineering changes and improvements are made from time to time. Therefore, some changes, modifications, and improvements may not be covered in this manual. If your unit operating characteristics or appearance differs from those described in this manual, please contact your Shel Lab dealer or distributor for assistance.
C
ONTACTING
A
SSISTANCE
Phone hours for Sheldon Technical Support are 6 am – 4:30 pm Pacific Coast Time (west coast of the United States, UTC -8). Please have the following information ready when calling or emailing
Technical Support: the model number and the serial number (see page 9).
EMAIL: [email protected]
PHONE: 1-800-322-4897 extension 4, or (503) 640-3000
FAX: (503) 640-1366
Sheldon Manufacturing, INC.
P.O. Box 627
Cornelius, OR 97113
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RECEIVING YOUR UNIT
I
NSPECT THE
S
HIPMENT
•
•
When a unit leaves the factory, safe delivery becomes the responsibility of the carrier.
Damage sustained during transit is not covered by the manufacturing defect warranty.
When you receive your unit, inspect it for concealed loss or damage to its interior and exterior. If you find any damage, follow the carrier’s procedure for claiming damage or loss.
1. Carefully inspect the shipping carton for damage.
2. Report any damage to the carrier service that delivered the unit.
3. If the carton is not damaged, open the carton and remove the contents.
4. The unit should come with an Installation and Operation Manual.
5. Verify that the correct number of accessories have been included.
Included Accessories SCO6WE
1 Copper Pan Token 1 Access Port
Stopper*
1 Ceiling Air Duct CO
2
Tubing Kit
5800529
1 Humidification Pan
7750514
4 Leveling Feet
5121514
1 Power Cord
9710500
Fill Port Stopper*
995-00015
6 Shelf Slides
PN 2700506
4 Shelf Standards
PN 1800510
3 Shelves
7750513
5121028 5170646 5121777
*The access and water fill stoppers ship installed in their respective ports on the unit.
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RECEIVING (CONTINUED)
O
RIENTATION
P
HOTO
Back of Unit
Fuse in Cord Inlet
CO
2
Gas In Port
Back of Unit
Fill Port
Water Jacket Ports
Siphon Port Anode Top
Side of Unit
CO
2
Sample Port and USB Port
Control Panel
Side of Unit
Access Port
Door Gasket
Chamber Door
Handle
Chamber
Gasket
Door Latch
Shelf Standard Rail
Humidification Pan
Chamber Door
Viewing Door (Glass)
Figure 1: SCO6WE
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RECEIVING (CONTINUED)
R
EGULATOR
The incubator must be connected to either a building CO
2 gas supply system or a supply cylinder (tank). A cylinder regulator is not included with the incubator, and
must be purchased separately. Please see the Accessories section on page 54 if
you wish to order one from Shel Lab.
Figure 2: CO
2
Regulator
R
ECORDING
D
ATA
P
LATE
I
NFORMATION
Locate the data plate inside the incubation chamber, on the top right corner of the chamber door.
The data plate contains the incubator model number and serial number. Enter this information below for future reference.
Date Plate Information
Model Number
Serial Number
R
EFERENCE
S
ENSOR
D
EVICES
Reference sensor devices or a combined device must be purchased separately in order to perform accuracy verifications or calibrations of the incubator temperature and CO
2
displays.
Reference devices must be accurate to at least 0.1°C and 0.1% CO
2
. The devices should be regularly calibrated, preferably by a third party. For best temperature results, use a digital device with a wired-connected temperature sensing probe that can be placed in the incubation chamber through the unit access port. For example: a wire thermocouple probe. For best CO
2
accuracy, use a calibrated digital gas analyzer with sample tubing that can be connected to the incubator external
CO
2
sample port.
Reference readings that avoid chamber door openings during verification and calibration eliminate subsequent waits for the chamber temperature and gas levels to re-stabilize before proceeding.
This also allows temperature and gas verifications or calibrations to be performed simultaneously.
Select probes suitable for the application temperature you will be calibrating or verifying the incubator displays at.
Alcohol thermometers are insufficient for conducting accurate temperature verifications and calibrations. Do not use a mercury thermometer. Never place a mercury thermometer in the
incubation chamber.
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INSTALLATION
I
NSTALLATION
C
HECKLIST
Carry out the steps and procedures listed below to install the unit in a new workspace location and prepare it for use. All procedures are found in the Installation section of this manual.
Pre-Installation
Procure a CO
2
gas supply for the incubator with a concentration suitable for your application.
Always use medical grade CO
2
.
Check that the required ambient conditions, ventilation, and spacing for the incubator are met,
•
Unit dimensions may be found on page 51
Check for performance-disrupting heat and cold sources in the environment, page 12
Check that a suitable electrical outlet and power supply is present, page 26
Install the Incubator in a suitable location
Review the lifting and handling instructions, page 13
Install the incubator in its workspace location, page 13
Make sure the incubator is level, page 13
Set up the Incubator for use
Clean and disinfect the incubator and accessories that will be placed in the incubation
•
Do not use deionized water to clean the unit or fill the water jacket, see page 13
Install the ceiling air duct in the incubation chamber, page 14
Install the shelving in the incubation chamber, page 15
Connect the incubator to the CO
2
Verify that the rubber stopper is installed in the access port inside the incubation chamber,
Fill the water jacket with 9.5 gallons (36 liters) of water, page 17
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INSTALLATION (CONTINUED)
R
EQUIRED
A
MBIENT
C
ONDITIONS
SCO incubators are intended for use indoors at room temperatures between 15
°C and 30°C (59°F and 86
°F), at no greater than an ambient 80% Relative Humidity (at 25°C / 77°F).
•
•
4 inches (10cm) minimum ventilation clearance between the incubator and walls or partitions.
2 inches (5cm) minimum clearance above the top of the incubator for unobstructed airflow.
Operating the unit outside these conditions may adversely affect its temperature range and
stability. For conditions outside of those listed above, please contact your distributor to explore other unit options suited to your laboratory or production environment.
E
NVIRONMENTAL
D
ISRUPTION
S
OURCES
When selecting a location to install the incubator, consider all environmental conditions that can affect the unit temperature performance. For example:
•
•
•
•
Proximity to ovens, autoclaves, and any device that produces significant radiant heat
Heating and cooling ducts, or other sources of fast-moving air currents
High-traffic areas
Direct sunlight
P
OWER
S
OURCE
R
EQUIREMENTS
When selecting a location for the unit, verify that each of the following requirements are satisfied:
The wall power source must match the power requirements listed on the unit data plate. These units are intended for 110 – 120 VAC 50/60 Hz applications at 6.0 amps.
• Supplied voltage must not vary more than 10% from the data plate rating. Damage to the unit may result if supplied voltage varies more than 10%.
• Wall power sources must be protective earth grounded and conform to all national and local electrical codes.
• Use a separate circuit to prevent loss of product due to overloading or circuit failure. The circuit must match or exceeded the amperage requirement listed on unit the data plate.
The unit must be positioned so that all end-users can quickly unplug the power cord in the event of an emergency.
•
•
The unit is provided with a 115 volt 15 Amp, 9ft 5 in (2.86m) NEMA 5-15P power cord.
The unit is provided with a 250V time-lag T 10 amp 5x20mm fuse located in the power cord receptacle (inlet).
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INSTALLATION (CONTINUED)
L
IFTING AND
H
ANDLING
The unit is heavy. Use appropriate powered lifting devices. Follow these guidelines when lifting and handling the unit:
•
•
•
•
Lift the unit only from its bottom surface.
Doors, handles, and knobs are not adequate for lifting or stabilization.
Restrain the unit completely while lifting or transporting so it cannot tip.
Remove all removable components, such as shelving, and secure all doors in the closed position during transfer to prevent shifting and damage.
I
NSTALL
I
NCUBATOR IN
L
OCATION
Install the unit in a workspace location that meets the criteria discussed in the previous entries of the Installation section.
L
EVELING
Install the leveling feet shipped with the unit. The unit must be level and stable for safe operation.
Insert one leveling foot into each of the four holes in the bottom corners of the unit.
Adjust the foot at each corner until the unit stands level and solid without rocking.
To raise a foot, turn it in a counterclockwise direction.
To lower a foot, turn it in a clockwise direction.
Figure 3:
Leveling Foot
Note: To prevent damage when moving the unit, turn each of the four leveling feet completely clockwise.
D
EIONIZED AND
D
ISTILLED
W
ATER
Do not use deionized water to clean or humidify the incubator, or fill the water jacket. Use of deionized water may corrode metal surfaces and voids the warranty. The manufacturer recommends the use of distilled water in the resistance range of 50K Ohm/cm to 1M Ohm/cm, or a conductivity range of 20.0 uS/cm to 1.0 uS/cm, for cleaning, humidifying, and water-jacketing applications.
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INSTALLATION (CONTINUED)
I
NSTALLATION
C
LEAN AND
D
ISINFECT
Cleaning and disinfecting the unit incubation chamber, shelving components and ceiling air duct now reduces the risk of contamination. The chamber and shelving were cleaned and disinfected at the factory, however, the unit may have been exposed to contaminants during shipping.
• Remove all protective wrappings from shelving components and the ceiling air duct prior to cleaning.
•
See the Cleaning and Disinfecting entry on page 39 for
information on how to clean and disinfect without damaging the incubator or its components.
Figure 4: Chamber Air
Duct Installation
Chamber Ceiling Air Duct
Back
I
NSTALL THE
C
HAMBER
A
IR
D
UCT
Note: Exercise caution to avoid striking the sensors and blower fan wheel on the chamber ceiling when installing the duct.
Note: The incubator must be turned off and unplugged when carrying out this procedure.
Install the ceiling air duct to ensure the even flow of air throughout the chamber space. This is essential for meeting the unit temperature uniformity specs in the chamber.
Carry out the following steps:
Place the duct in the chamber. a. The back of the duct should be toward the rear of the chamber b. The legs on the duct should face down toward the chamber ceiling, next to the left and right chamber walls
Move the air duct to the top of the chamber.
Seat the duct-mounting legs carefully in the shelf-standard clips.
Step 2
Step 1
Front
Step 3
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INSTALLATION (CONTINUED)
S
HELVING
I
NSTALLATION
Note: Always install the copper token in the humidification pan. Copper is known to have antimicrobial properties that retard the growth of microorganisms in the pan.
Install the shelving and humidification pan in the in the incubation chamber.
Install the shelf standard rails. a. Align the keyhole slot of the standard with the mounting peg on the side of the chamber wall. b. Mount the shelf standard.
Install the shelf slides. a. Insert the shelf slide into the shelf standard using a rocking motion. b. The shelf slide will sit level when correctly installed.
Install the shelves. a. Slide into position.
Install the humidification pan. a. Place the copper token in the humidity pan. b. Secure the token using the clip on the bottom of the pan. c. Place the pan on the chamber floor.
Figure 5: Shelving Installation
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INSTALLATION (CONTINUED)
C
ONNECT TO THE
CO
2
S
UPPLY
Note: Always use medical grade CO
2.
Use of non-medical grade CO
2 risks introducing contaminants into the chamber may damage the incubator, and voids the manufacturing defect warranty.
CO
2
Regulator SCO6WE
Figure 6: CO
2
Cylinder
Connection
HEPA Filter
The incubator may be connected to either a building supply source or a supply cylinder.
Two-Stage Regulators: If connecting to a supply cylinder always use a two-stage CO
2
pressure
regulator. Be aware that some single-stage regulators have 2 gauges. Make certain your regulator is a two-stage regulator. Precise regulation of the gas input flow is vital for the incubator performance.
Connect to the CO
2
supply
Attach the CO
2 regulator to a medical grade CO
2 cylinder, if using a cylinder supply.
Set the wall source control or cylinder regulator to 15 - 20 Pounds per Square Inch (psi).
Do not exceed 25 psi.
PSI
15 - 20 psi
Megapascals
0.103 - 0.137 Mpa
Kilopascals
103.42 - 137.89 Kpa
Bar
1.03 - 1.378 bar
Figure 7: Gas
Tubing Kit
Remove the dust cover from the CO
2
–to-Chamber port on the back of the unit.
Connect the gas tubing to the incubator and regulator or wall source. a. Connect the black tubing to the regulator or wall source. b. Connect the clear tubing to the CO
2
to Chamber port on the back of the incubator.
Do not initiate a flow of CO
2
to the incubator at this time.
End of procedure
Figure 8: CO
2 to Chamber
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INSTALLATION (CONTINUED)
A
CCESS
P
ORT
S
TOPPER
Verify that that a port stopper is installed in the access port on the right side of the unit.
A rubber access port stopper is shipped in the port. The stopper must be
installed inside the chamber to obtain the best temperature uniformity and prevent condensation from forming inside the port.
Figure 9: Port Stopper in the Access Port
F
ILL THE
W
ATER
J
ACKET
The water jacket requires 19 gallons (72 liters) to provide sufficient thermal insulation for the incubator to operate at its stated temperature specifications.
Fill Port
19 Gallons
(72 Liters)
The manufacturer recommends the use of distilled water or
filtered tap water if possible. Please see the Anode and
Water Quality entry in the maintenance section on page 50.
Use of a funnel or hose while filling the jacket is strongly recommended.
Locate the water fill port on the back right side of the incubator.
Remove the port stopper. a. Place the port stopper in a location where it will be easy to find.
Fill the jacket with 19 gallons (72 liters) by pouring water in through the fill port.
Place the stopper back in the port.
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INSTALLATION (CONTINUED)
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GRAPHIC SYMBOLS
Each incubator is provided with multiple graphic symbols on its exterior and internal surfaces.
These symbols identify hazards, and the functions of the adjustable components, as well as important notes in the user manual.
Symbol Definition
Indicates that you should consult your user manual for further instructions
Indique que l'opérateur doit consulter le manuel d'utilisation pour y trouver les instructions complémentaires
Indicates temperature
Repère température
Indicates the Over Temperature Limit system
Indique le système de dépassement de temperature
Indicates AC Power
Repère le courant alternatif
Indicates I/ON and O/OFF
I repère de la position MARCHE de l'interrupteur d'alimentation
O repère de la position ARRÊT de l'interrupteur d'alimentation
Indicates protective earth ground
Repère terre électrique
Indicates Up and Down respectively
Touches de déplacements respectifs vers le HAUT et le BA
Indicates Potential Shock Hazard
Signale danger électrique
Indicates the unit should be recycled (Not disposed of in land-fill)
Indique l’appareil doit être recyclé (Ne pas jeter dans une décharge)
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GRAPHIC SYMBOLS (CONTINUED)
Symbol Definition
Indicates relative humidity
Indique humidité relative
Indicates water level low
Indique faible niveau d'eau
Indicates CO
2
gas
Indique gaz CO
2
Indicates the incubator heater is active.
L'élément chauffant est la production de chaleur.
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CONTROL PANEL OVERVIEW
Figure 10: Control
Panel
Power Switch
The round, black main power switch controls all power to the incubator and its systems. “I” is the on position, and “O” the off.
Over Temperature Limit
This graduated dial sets the heating cut off point for the OTL temperature limit system. The OTL system prevents unchecked heating of the chamber in the event of a failure of the main digital
controller. For more details, please see the Over Temperature Limit System description in the
Theory of Operations (page 25).
The red Over Temp Activated light illuminates when the Over Temperature Limit system cuts off heating by rerouting power away from the heating elements.
Temperature Control and Display
Labeled Set Temperature °C, this display shows the current air temperature in the incubation chamber accurate to within 0.1
°C. The arrow buttons can be used to adjust the temperature set point, or place the display in its temperature calibration mode and then enter a display value correction.
Red LED alarm indicators marked High and Low illuminate when the temperature deviates by
±1°C or greater from the current set point. The yellow LED marked Mute illuminates whenever
The green indicator labeled Heating Activated illuminates whenever the temperature control system is heating the incubation chamber.
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CONTROL PANEL OVERVIEW (CONTINUED)
CO
2
Display
Labeled Set CO
2,
this display shows the concentration of CO
2
in the incubation chamber as a percentage of the chamber atmosphere. The display has a range of OFF to 20% and an accuracy of 0.1%. The display shows “LO” until the CO
2 sensor registers a concentration in the chamber greater than 0%. When initially injecting CO
2
into the chamber a few minutes may be required to build up a sufficient concentration to register.
The UP / DOWN arrow pad can be used to adjust the CO
2
concentration set point and to mute audible gas deviation alarms. The control can also place the CO
2
display in its calibration mode, and be used to enter a calibration adjustment.
Red LED alarm indicators marked High and Low illuminate whenever CO
2
deviations of ±1% or greater from the set point take place. The yellow LED marked Mute illuminates whenever an
audible deviation alarm is being muted. See the Muting the Audible CO
of the Operations section for more information.
The green CO
2
Injecting indicator illuminates when the incubator is injecting CO
2
into the incubation chamber. Injections are accompanied by a click that is the CO
2 solenoid opening and then closing.
Water Jacket Low
The red indicator marked Water Jacket Low illuminates when the water level of the jacket has fallen to a level that impacts the incubator temperature uniformity and stability. Add water to the water-
section for instructions.
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OPERATION
T
HEORY OF
O
PERATION
The SCO6WE is engineered to provide constant temperature CO
2
incubation environments that is passively humidified to prevent the premature drying of sample media. The incubator can obtain a stable, uniform temperature in its chamber, ranging from the room temperature (ambient) +5°C up to 60°C for incubation applications. The CO
2 range is a 0 – 20% concentration. Filling the humidification pan will result in a relative humidity (RH) of 90 – 95% in the chamber.
Each incubator features a glass viewing door that allows visual inspection of samples without compromising the chamber CO
2 or humidity environment.
Heating
When powered, the unit heats to and maintains the incubation chamber air temperature at the currently programed temperature set point. An internal microprocessor stores the temperature set point. The microprocessor board is wired to a solid-state temperature probe located on the chamber interior right wall. When the processor detects that the chamber temperature has dropped below the temperature set point, it pulses power to heating elements on the surface of the waterjacket insulation space, and in the outer chamber door. Heat from the elements on the jacket propagates through water inside the jacket to the chamber wall, and then to the chamber airspace.
During normal operations, with the doors closed, most heating pulses correct for deviations of less than 0.1°C.
The processor employs proportional-integral-derivative analytical feedback-loop functions when measuring and controlling the chamber air temperature. PID-controlled heating pulse intensities and lengths are proportional to the difference between the measured chamber temperature and the current set point. The frequency of pulses are derived from the rate of change in that difference.
The integral function slows the rate of pulses when the temperature nears the set point to avoid overshooting.
Each incubator relies on natural heat radiation for cooling. The incubator can achieve a low-end temperature of the ambient room temperature +5°C.
The chamber door is self-heating to bolster the thermal uniformity and stability of the chamber, and to minimize condensation on the inner glass viewing door. The protection provided by the viewing door against temperature and CO
2
disruptions is of limited duration. Minimize sample viewing times with chamber door open, when possible.
Insulation – Water Jacket
Insulation is provided by the water-filled jacket surrounding the incubation chamber on the top, bottom, back, and left and right sides. The mass of the water, once heated to the set point, provides a high degree of temperature uniformity in the chamber, as well as thermal inertia. In other words, the chamber will retain heat in the event of a power outage for significantly longer than an airjacketed incubator. However, the water jacket does require a longer time to come up to temperature when placing the unit in operation from a cold state.
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OPERATION (CONTINUED)
CO
2
Atmosphere
The same microprocessor board that controls the chamber temperature also manages the gas concentration of CO
2
in the chamber atmosphere by operating an internal injection solenoid valve connected to the gas input ports. The processor monitors CO
2 concentration level in the incubator using an infrared sensor located behind the chamber ceiling duct. The sensor operates on the principle that a specific frequency set of infrared light is absorbed by CO
2
. The more CO
2
present in chamber, the more of that band of infrared is absorbed. The sensor is only sensitive to CO
2
, so measurement accuracy is consistent, regardless of the presence of other gasses in the incubator.
The processor employs proportional-integral-derivative analytic feedback-loop functions when measuring and controlling the CO
2
concentration. When the PID are active, injection lengths are proportional to the difference between the measured concentration and the set point. The frequency of injections is derived from the rate of change in the difference. Integrator feedback slows the rate of injection as the concentration approaches the set point, which helps prevent overshoots. When the chamber concentration is stable CO
2
injections take place in small bursts to correct for deviations less than 0.1%. The incubator is not provided with a means to actively remove CO
2 from the chamber atmosphere.
Humidification
Passive humidification is provided by filling the humidification pan included with the unit. The pan is then placed on the heated chamber floor. Evaporation driven in part by heating raises the relative humidity percentage (RH%) of the chamber. A copper token included with the pan helps to significantly slow the growth of microbiological populations in the humidification water supply.
The incubator must be operated humidified in order to achieve its stated temperature uniformity specification.
Physical and Data Access
An access port on the right side of the unit allows sensors such as, thermocouples and humidity meter solid state probes, to be inserted and left in the chamber without compromising the CO
2 atmosphere or temperature performance. An atmosphere sample port for independently verifying the CO
2
concentration in the chamber is provided on the left side of the control panel. A USB-style serial port outputs CO
2
and temperature levels once per minute as a digital log line. Please see the
Data Output entry on page 37 for more details.
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OPERATION (CONTINUED)
The Over Temperature Limit System (OTL)
When set, the mechanical OTL heating cutoff system prevents runaway heating in the incubation chamber. The OTL operates independently of the microprocessor and is provided with a separate, hydrostatic temperature sensor probe located in the chamber. In the event the chamber air temperature exceeds the current OTL setting, the OTL routes power away from the heating elements. The OTL will continue to prevent heating until the temperature drops below its limit setting. The Over Temperature Limit is set by the end-user, typically at approximately 1°C above the application temperature set point.
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OPERATION (CONTINUED)
Note: From a cold start, the incubator requires 12 hours to come up to and stabilize at temperature and humidity levels prior to loading samples. Stabilization safeguards samples.
P
UT THE
I
NCUBATOR INTO
O
PERATION
Carry out the following steps and procedures to put the incubator into operation after installing it in a new workspace environment:
Optional: A clean and disinfected thermocouple probe for performing the optional temperature display accuracy verification may be inserted through the access port now. a. This saves time by allowing the incubation chamber temperature to stabilize undisturbed prior to the verification procedure.
b. See the Temperature Display Accuracy Verification procedure on page 32
for the correct introduction and placement of the thermocouple probe.
Verify that the workspace power supply and the incubator power requirements listed on the unit data plate have been matched.
a. See the Power Source Requirement entry on page 26.
Attach the power cord that came with the incubator to the power inlet receptacle on the back of the unit.
Plug the power cord into the workspace supply outlet.
Place the Power switch in the on ( I ) position.
Perform the following procedures in sequence to finish preparing the incubator:
Humidifying the Incubator page 27
Set the Temperature Set Point page 25
Allow the incubator to heat undisturbed for 10 hours before continuing.
Open the CO
2
supply control or gas regulator so it supplies a flow of
15 - 20 psi, as per the supply description on page 16.
After setting the set point, wait for an additional 2 hours for a CO
2 concentration to establish and stabilize in the chamber, and for the unit to finish stabilizing thermally undisturbed with the doors closed.
Optional, Verify Temperature Display Accuracy page 32
Set the Over Temperature Limit page 36
+
=
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OPERATION (CONTINUED)
H
UMIDIFY THE
I
NCUBATOR
Note: Humidifying the chamber helps slow the drying of samples in open, “breathable” containers
Fill the humidification pan in the incubation chamber.
• The humidification pan must be filled in order for the incubator to achieve its stated temperature uniformity specification.
• The pan must be placed on the chamber floor. The floor is heated and helps evaporation to raise the humidity level to 90 – 95% relative humidity.
• Always place and secure the copper token in the pan to slow the growth of microbiological populations in humidification water supply.
•
•
•
Regularly clean and disinfect the pan.
Refill as needed, and change the water in the pan at least once per week.
Use of chemical disinfectants added to the pan may alter the surface tension of the water.
This may significantly reduce the rate of evaporation and impact the humidity level of the incubator chamber.
• Never use deionized water to humidify the incubator.
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OPERATION (CONTINUED)
S
ET THE
T
EMPERATURE
S
ET
P
OINT
Perform the steps below to change the set point to the operational temperature you will be using during your incubation application. The incubator comes from the factory with a set point of 37
°C.
Note: The visual example below depicts adjusting the incubator set point from 35°C to a 37°C application temperature.
Set Temperature Set Point
Turn the OTL control clockwise to the maximum, if not already set to max. a. This prevents the Over Temperature system from interfering with setting the set point.
Push and release the Up or Down button on the Set Temperature control panel to activate the temperature set point mode. a. The temperature display will briefly flash the letters “SP” to indicate a Set Point is about to be displayed. b. The digital display will then dim and showing the blinking, adjustable temperature set point.
Use the Up and the Down button to enter a new set point. a. If neither button is pressed within 5 seconds, the temperature display will stop blinking and return to displaying the current temperature of the incubator.
Wait 5 seconds after entering the new set point. a. The display will stop flashing. The new set point is now saved in the microprocessor controller. b. The incubator will automatically heat or cool adjust to achieve the new set point. c.
See the Set the OTL procedure
on page 36 for how to set the OTL system once
the incubation chamber has stabilized at your application temperature set point, and after you have performed any display verifications or calibrations.
End of Procedure
Set Temperature °C
Set Point Indicator
Set Temperature °C
Initial Set Point
Set Temperature °C
New Set Point
Set Temperature °C
Heating to Adjusted Set Point
28 | P a g e
OPERATION (CONTINUED)
M
UTING THE
A
UDIBLE
T
EMPERATURE
A
LARM
An audible and visual deviation alarm activates if the incubation chamber temperature deviates by 1
°C above or below the temperature set point. The low deviation audible alarm has a delay of 15 minutes. This prevents the low alarm from sounding whenever the chamber doors are opened, causing a short drop in temperature.
Carry out the following step to mute active high or low deviation alarms.
Press and hold either the Up or Down arrow on the Temperature Control panel, until the amber Mute LED illuminates and the audible alarm shuts off.
•
•
The audible alarm component will remain muted for the duration of the
current temperature deviation. The visual alarm indicator will remain illuminated.
Any new deviation of
±1°C or greater will reactivate the audible alarm.
Figure 11: High
Alarm Muted
A
UTOMATIC
D
OOR
C
UTOFF
Whenever the incubation chamber outer door is opened, the incubator stops the flow of CO
2
into the chamber, depowers the heater element, and ceases operation of the internal blower fan. This limits the amount of CO
2
released into the workspace around the incubator. It also prevents the heater from attempting to counteract the continual inflow of cooler air, which would cause a significant heat spike once the door is closed. Normal CO
2
injections, heating, and fan operation all resume automatically when the outer door is closed.
29 | P a g e
OPERATION (CONTINUED)
S
ET THE
CO
2
S
ET
P
OINT
The incubator comes from the factory set to Off. Set the CO
2
set point to that of your application.
The gas supply must continually deliver 15 - 20 psi while establishing and maintaining a CO
2
enriched chamber atmosphere. A CO
2
flow to the chamber must be started a minimum of 2 hours prior to the start of a display verification or calibration, or prior to loading samples in the chamber.
The CO
2
display will read “LO” until enough CO
2 has built up for the sensor to register a concentration greater than 0%.
Note: The example below represents adjusting the CO
2
set point from off to 5%.
Set CO
2
Set Point
Press either the Up or Down arrow button on the CO
2
panel. a. The display will flash the letters “SP” for set point b. A flashing, adjustable CO
2
set point will appear in the display
Note: The display will automatically exit the adjustment mode after 5 seconds of inactivity, with the last shown set point value saved.
Use the Up or the Down arrow keys to adjust the set point to your application CO
2 concentration.
Wait 5 seconds after entering the set point. a. The display will stop flashing, and the set point is now saved in the controller processor b. The chamber will now automatically inject CO
2
or allow the current level to decay in order to achieve your set point c. The display will revert to showing the current chamber concentration
Note: The CO
2
display and injections can be set to off when in the set point adjustment mode. Hold the down arrow after the blinking set point appears until the display reads “OFF”. The incubator will then cease injecting CO
2
.
End of procedure
30 | P a g e
Set CO
2
%
Set CO
2
%
Initial Set Point
Set CO
2
%
New Set Point
Set CO
2
%
CO
2
Injecting to achieve the new set point.
OPERATION (CONTINUED)
M
UTING THE
A
UDIBLE
CO
2
A
LARM
Visual indicator alarms illuminate if the chamber CO
2
level deviates 1% above or below the CO
2 set point. An audible alarm sounds immediately for a high deviation. The low deviation audible alarm will sound after the visual low indicator alarm has been continually illuminated for 15 minutes. This delay prevents the alarm from sounding whenever a door opening creates a short-lived drop in gas concentration.
Carry out the step below to mute an active audible deviation alarm.
To mute an alarm, press and hold the CO
2
Up or the Down arrow button until the amber
Mute LED illuminates.
• The alarm will stay muted for the duration of the current temperature deviation.
• Another deviation of 1% will reactivate the audible alarm.
Figure 12: Low
Gas Alarm Muted
Figure 13: Gas Alarm Timeline
N
O
G
AS
S
UPPLY
A
LARM
(NGS)
If the Low Gas deviation indicator is active for longer than 20 minutes, a second alarm will activate.
SET CO
2
• The letters “ngS” will appear in the CO
2
display to indicate No Gas Supply.
• The alarm will remain active even if the incubator is turned off and turned back on.
Figure 14: No Gas
Supply
The NGS Alarm will remain on until CO
2
is restored to the chamber. It may take 2 or more minutes of CO
2
inflow to establish a concentration percentage (%) high enough to deactivate the alarm.
31 | P a g e
OPERATION (CONTINUED)
T
EMPERATURE
A
CCURACY
V
ERIFICATION
Note: Performing a temperature accuracy verification requires a temperature reference device.
Please see the Reference Sensor Devices entry on page 10 for the device requirements.
Optional: This procedure verifies the accuracy of the incubator temperature display against the actual chamber air temperature as measured by a reference sensor device. Perform the procedure if required by your laboratory or production protocol. The unit was calibrated at the factory at 37ºC.
If a difference between the actual and displayed temperatures is discovered, perform a temperature
calibration. Please see the Calibrate Temperature Display procedure on page 42 in the User
Maintenance section.
Humidity
Perform the verification with the chamber fully humidified. The humidity level of the chamber impacts its temperature uniformity. 8-hours are required for the unit to achieve and stabilize at its operational relative humidity level of 90 – 95%, from a dry state.
CO
2
A CO
2
display verification may be performed concurrently with the temperature verification.
Probes
Reference device sensing probes may be introduced through the access port. Carefully place the port stopper over any probe wires. The probe may also be introduced through the chamber door space. Use non-stick, nonmarking tape to secure the wires and probe head, and to seal any gaps.
The door must close and latch fully.
Place the sensor probe head of the temperature reference device as close as possible to the geometric center of the incubation chamber. A thermocouple sensor probe sleeve may be taped to the shelving, as long as the exposed copper end is 2 inches (5cm) away from the shelf (see
Figure 16). An exposed sensor probe in direct contact with the shelving
may experience heat sinking, which can result in an inaccurate temperature reading.
Temperature Stability
After introducing and placing the temperature probe, allow the incubator to operate undisturbed and humidified for at least 24-hours prior to performing the verification.
The incubator must operate humidified at its verification temperature set point for at least 1 hour with no fluctuations of ±0.1°C or greater in order to be considered stabilized. Failure to wait for stabilization will result in an inaccurate verification. If the chamber door is opened during the verification the chamber must be allowed to re-stabilize before continuing.
Figure 15: Introducing a sensor probe through the access port.
Figure 16: Probe End 2 inches (5cm) From Shelf
Surface
32 | P a g e
OPERATION (CONTINUED)
Verifying the Temperature Display Accuracy
Once the chamber temperature has stabilized, compare the reference temperature device and incubator display readings. a. If the readings are the same, or if a difference between the two (2) falls within the acceptable range of your protocol, the display is accurately showing the chamber air temperature.
The Temperature Verification procedure is now
complete. b. See step 2 if a difference falls outside the acceptable range of your protocol.
If there is an unacceptable difference, a display temperature calibration
must be performed to match the display to the reference device.
a. Please see page 42 in the User Maintenance section.
Reference Device
Set Temperature °C
Reference Device
Set Temperature °C
End of procedure
33 | P a g e
OPERATION (CONTINUED)
CO
2
A
CCURACY
V
ERIFICATION
Note: Performing a CO
2
display accuracy verification requires a gas reference device. Please see
the Reference Sensor Devices entry on page 10 for the device requirements.
Optional: The CO
2
display was calibrated at the factory at a 5% concentration. A display accuracy verification may be performed when preparing the incubator for use, or if required by your laboratory or production protocol. The verification procedure compares the chamber CO
2 level as measured by the incubator with the actual level, as provided by a calibrated reference device.
If a difference between the actual and displayed CO
2 concentrations is discovered, perform a CO
2
display calibration. Please see the Calibrate CO
Display procedure on page 46 in the User
Maintenance section.
Temperature
A CO
2
display verification may be performed simultaneously with the temperature display verification, as long as the chamber door is not opened during either procedure. The incubation chamber should be heated to and running at your application temperature, as temperature drives gas diffusion in the chamber.
Humidity
The incubator should be allowed to come up to humidity in the chamber prior to performing a CO
2 verification. Relative humidity affects CO
2 distribution due to its influence on the chamber atmosphere temperature.
Probes
Connect a CO
2
reference device sample tube to the sample port, located on the left side of the control panel.
Gas Stability
Allow the unit to operate undisturbed to run for at least 12 hours for heat and humidity stability (for example, overnight) prior to performing the verifications. A CO
2
flow to the chamber may be started 2 hours prior to the start of the verification.
Figure 17: CO
Sample Port
Prior to a verification, the chamber must operate at its CO
2
set point for at least 1 hour with no
fluctuations of ±0.1% or greater in order to be considered stabilized. Failure to wait for stabilization will result in an inaccurate verification. If the chamber door is opened during the verification the chamber must be allowed to re-stabilize before continuing.
2
Continued on next page
34 | P a g e
OPERATION (CONTINUED)
Verifying the CO
2
Display Accuracy
Once the chamber has stabilized with no fluctuations, compare the gas reference device and chamber CO
2
display readings. a. If the readings are the same, or if a difference between the two (2) falls within the acceptable range of your protocol, the display is accurately showing the chamber CO
2 concentration. The CO
2
Verification procedure is now
complete. b. See step 2 if a difference falls outside the acceptable range of your protocol.
If there is an unacceptable difference, a CO
must be performed to match the display to the reference device.
a. Please see page 46 in the User Maintenance section.
Reference Device
Set CO
2
Reference Device
Set CO
2
End of procedure
35 | P a g e
OPERATION (CONTINUED)
Note: Test the OTL system at least once per year for functionality.
S
ET THE
O
VER
T
EMPERATURE
L
IMIT
This procedure sets the Over Temperature Limit heating cutoff to approximately 1
˚C above the current chamber temperature. Perform the steps below once the incubator has run with no temperature fluctuations at your application temperature set point for at least 1 hour.
Set OTL
Turn the Set Over Temperature Limit control dial clockwise to the maximum position, if it is not already set to maximum.
Turn the Over Temperature Limit control dial counterclockwise until the red Over Temperature Limit Activated light illuminates.
Slowly turn the dial clockwise until the Over Temperature Limit
Activated light turns off. Stop turning the control. a. The Over Temperature Limit is now set approximately 1
˚C above the current chamber temperature.
Optional: You may turn the dial slightly to the left to bracket in closer to the set point temperature. This sets the OT Limit nearer to the current chamber temperature.
Example
Leave the OTL dial set just above the activation point.
If the OTL activates sporadically, turn the dial very slightly to the right (clockwise) one time.
If the OTL continues activating, check for ambient sources of heat or cold that may be adversely impacting the unit temperature stability. Check if any powered accessories in the chamber are generating heat. If you can find no sources of external or internal temperature fluctuations, contact
Tech Support or your distributor for assistance.
End of Procedure
36 | P a g e
OPERATION (CONTINUED)
L
OAD THE
I
NCUBATOR
Place items on the shelves inside the incubation chamber as evenly spaced as possible. Proper spacing allows for maximum air circulation and a high degree of temperature uniformity. Leave 1 inch (2.5cm) between sample containers and the chamber walls.
This is the final step in the Preparing the Incubator procedure .
A
CCESSORY
C
OMPATIBILITY
Make sure any accessory equipment used inside the incubation chamber can safely and effectively operate within your selected range of temperature, humidity, and CO
2
levels. Some equipment types such as, stirrers or shakers, can generate heat sufficient to disrupt the thermal uniformity and stability of the chamber.
D
ATA
O
UTPUT
C
APABILITIES
The incubator generates data outputs describing temperature and CO
2
levels as a digital log line, once per minute. These outputs are transmitted through a USB-style RS232 serial port located on the left side of the incubator.
USB-Style Serial Port Output
Channel
C1
C3
Example logline output: C1=37.0 C3=5.0
Parameter
Temperature
CO
2
A software driver and data logging package for the port can be downloaded from the Shel Lab website. To download the software, visit the product page of any Shel Lab SCO incubator, and click on the large USB bar icon titled “USB Software for CO
2
Incubators” located approximately halfway down the page. http://shellab.com/product/sco6we-shel-lab-basic-co2-water-jacketed-incubator-infrared-irsensor-6-cu-ft-120v/
37 | P a g e
OPERATION (CONTINUED)
C
ONDENSATION AND THE
D
EW
P
OINT
Relative humidity inside the incubator chamber should never be allowed to exceed 95%.
Exceeding this thresholds will likely result in condensation, possible leaks around the incubator, and may cause corrosion damage, if allowed to continue for any significant length of time
Condensation takes place whenever the humidity level in the incubator chamber reaches the dew point. The dew point is the level of humidity at which the air cannot hold more water vapor. The warmer the air, the more water vapor it can hold.
As the level of humidity rises in an incubation chamber, condensate will first appear on surfaces that are cooler than the air temperature. Near the dew point, condensate forms on any item or exposed surface even slightly cooler than the air. When the dew point is reached, condensate forms on nearly all exposed surfaces.
Managing condensation primarily depends on either lowering the humidity level or increasing the air temperature in the incubator chamber.
Note: Rising or falling air pressure from weather will adjust the dew point up and down in small increments. If the relative humidity in the incubation chamber is already near the dew point, barometric fluctuations may push it across the dew point threshold.
Note: Thin air at higher altitudes holds less humidity than the denser air found at or near sea level.
If excessive condensate has appeared in the incubation chamber, dry the chamber interior and check the following.
•
•
•
• Verify that the access port stopper is in place, on the inside of the incubation chamber and not the unit exterior.
Make sure samples on the shelves are evenly spaced to allow for good airflow.
Ensure the chamber door is closing and latching properly.
Are frequent or lengthy chamber door openings causing significant temperature disruptions and chilling the chamber surfaces? If so, reduce the number of openings.
• Are there are too many open containers of evaporating sample media in the chamber? If so, reduce the number of open sample containers.
• Does the ambient humidity in the room exceed the stated operating range of 80% relative environmental humidity? If so, lower the room humidity.
• Is the incubator exposed to an external flow of cold air such as, an air-conditioning vent or a door to a cooler hallway or adjacent room? Block or divert the air, or reposition the unit.
• Check the door gaskets for damage, wear, or signs of brittleness or dryness. Arrange for replacement of the gaskets if damaged or excessively worn.
38 | P a g e
USER MAINTENANCE
Warning
: Prior to any maintenance or cleaning of this unit, disconnect the power cord from the power supply.
Avertissement
: Avant d'effectuer toute maintenance ou entretien de cet appareil, débrancher le cordon secteur de la source d'alimentation.
C
LEANING AND
D
ISINFECTING
If a hazardous material or substance has spilled in the incubator, immediately initiate your site’s
Hazardous Material Spill Containment protocol. Contact your local Site Safety Officer and follow instructions per the site policy and procedures.
•
•
•
The incubator chamber should be cleaned and disinfected prior to first use.
Periodic cleaning and disinfection are required to prevent microbiological contamination.
•
Do not use spray on cleaners or disinfectants. These can leak through openings and coat electrical components.
Do not use cleaners or disinfectants that contain solvents capable of harming paint coatings or stainless steel surfaces.
• Do not use chlorine-based bleaches or abrasives; these will damage the chamber
liner.
• Consult with the manufacturer or their agent if you have any doubts about the compatibility of decontamination or cleaning agents with the parts of the equipment or with material contained in it.
Warning
: Never clean the unit with alcohol or flammable cleaners.
Avertissement:
Ne jamais nettoyer l'appareil à l'alcool ou avec des nettoyants inflammables.
Cleaning
Remove all removable chamber components and accessories (shelves, racks, and any additional items).
Clean the chamber interior with a mild soap and water solution, including all corners.
Take special care when cleaning chamber sensor probes located at the rear of the chamber on the back wall.
Clean all removable accessories and components.
Clean and disinfect any attached sample tubing and replace if discoloring is present.
Rinse the chamber surfaces and shelving with distilled water and wipe dry with a soft cloth.
Do not use deionized water.
39 | P a g e
USER MAINTENANCE (CONTINUED)
Disinfecting
Note: Contact your local Site Safety Officer for detailed information on the disinfectants compatible with your cultivation or culturing applications.
Disinfect the incubation chamber on a regular basis. For maximum effectiveness, disinfection procedures are typically performed after cleaning and removal of gross matter contamination.
Perform the following steps to disinfect the incubator:
Turn the unit off. Open all doors and carry out your laboratory, clinical, or production space disinfection protocol.
If permitted by your protocol, remove all interior accessories (shelving and other nonattached items) from the chamber when disinfecting.
Gas concentrations from evaporating disinfecting agents can inhibit growth or cause metabolic symptoms in microbiological sample populations. a. Make sure chlorines, amphyls, quaternary ammonias, or any other overtly volatile disinfecting agents have been rinsed or otherwise removed from the chamber surfaces, prior to placing samples in the chamber.
Disinfect the incubation chamber using commercially available disinfectants that are noncorrosive, non-abrasive, and suitable for use on stainless steel surfaces. a. Disinfect all surfaces in the chamber, making sure thoroughly disinfect the corners. b. Do not disinfect the sensor heads.
When disinfecting external surfaces use disinfectants that will not damage painted metal or plastic.
M
INIMIZING
C
ONTAMINATION
E
XPOSURE
The following are suggestions for minimizing exposure of the incubator chamber to potential contaminants.
• Maintain a high air quality in the laboratory workspaces around the incubator.
• Avoid placing the incubator near sources of air movement such as doors, air vents, or high traffic routes in the workspace.
• Minimize the number of times the incubator chamber door is opened during normal operations.
40 | P a g e
USER MAINTENANCE (CONTINUED)
“IN”
G
AS
L
INES AND
HEPA F
ILTERS
Replace the in-line gas HEPA filter once per year or when a filter is noticeably discolored.
• HEPA filters are directional, and must be installed facing in the correct direction. The word “IN” is stamped on the rim of the filter assembly on the side that faces toward the gas supply.
• Gas lines should be replaced when cracking, brittleness, permanent kinking, or other signs of damage are present. Please
“IN”
Figure 18: In-Line Gas Filter
S
TORAGE OF THE
I
NCUBATOR
Perform the following steps if the incubator will be out of use for more than 24 hours to prevent microbiological contamination such as, fungus or mold.
Depower the incubator.
Disinfect and clean if required by your laboratory protocol, or if the chamber has been exposed to pathogenic microorganisms.
Use a soft cloth to dry the chamber surfaces.
M
AINTAINING
A
TMOSPHERIC
I
NTEGRITY
Periodically, inspect the door latch, trim, catch, and gaskets for signs of deterioration. Failure to maintain the integrity of the door system shortens the life span of the incubator.
E
LECTRICAL
C
OMPONENTS
Electrical components do not require maintenance. If the incubator fails to operate as specified,
please contact your distributor or Technical Support for assistance (please see page 7).
41 | P a g e
USER MAINTENANCE (CONTINUED)
C
ALIBRATE THE
T
EMPERATURE DISPLAY
Note: Performing a temperature display calibration requires a temperature reference device.
Please see the Reference Sensor Devices entry on page 10 for device requirements.
Temperature calibrations are performed to match the incubator temperature display to the actual air temperature inside the incubation chamber. The actual air temperature is supplied by a calibrated reference sensor device. Calibrations compensate for drifts in the unit microprocessor controller as well as those caused by the natural material evolution of the sensor probe in the humidified and heated chamber space. Calibrate as often as required by your laboratory or production protocol, or regulatory compliance schedule.
Humidity
Humidity affects temperature uniformity and stability in the incubation chamber. The chamber must be humidified for at least 24 hours in order to conduct an accurate calibration.
CO
2
A CO
2
calibration may be conducted simultaneously with a temperature calibration as long as the
chamber doors are not opened during either procedure.
Probe
A reference device sensor probe may be introduced through the access port.
Carefully place the port stopper over any probe wires (see Figure 19). Probes
may also be introduced through the chamber door space. Use non-stick, nonmarking tape to secure the wires and probe heads and seal any gaps. The door must close and latch fully.
Place the sensor probe of the temperature reference device inside as close as possible to the geometric center of the chamber. A thermocouple sensor probe sleeve may be taped to the shelving, as long as the exposed copper end is 2
inches (5cm) away from the shelf (see Figure 20). An exposed sensor probe in
direct contact with the shelving may experience heat sinking, which can result in an inaccurate temperature reading.
Stability
From a cold start, the incubator must operate humidified and at its application temperature set point for at least 24 hours in order to stabilize. Prior to a calibration it must operate for at least 8 hours undisturbed with the both doors closed. A common practice is to place the temperature sensor probe in the chamber, allow the unit to operate and stabilize overnight, and then conduct the calibration the next morning.
The chamber is considered stabilized when it has operated for 1 hour with no fluctuations ±0.1°C or greater. Failure to wait for stabilization will result in an inaccurate calibration and incubator temperature display reading.
Figure 19: Introducing a sensor probe through the access port.
Figure 20: Probe End 2 inches (5cm) From Shelf
Surface
42 | P a g e
USER MAINTENANCE (CONTINUED)
Temperature Calibration
Once the chamber temperature has stabilized, compare the reference device and temperature display readings. a. If the readings are the same, or if a difference between the two (2) falls within the acceptable range of your protocol, the display is accurately showing the chamber air temperature.
The Temperature Calibration procedure is now complete. b. If a difference falls outside of your protocol range, advance to step 2.
A display calibration adjustment must be entered to match the display to the reference device. See next step.
Reference Device
Set Temperature °C
Reference Device
Set Temperature °C
Set Temperature °C
Place the temperature display in its calibration mode. a. Press and hold both the UP and DOWN temperature arrow buttons simultaneously for approximately 5 seconds. b. Release the buttons when the temperature display shows the letters “CO”. The display will then begin flashing the current
temperature display value.
Note: If an arrow button is not pressed for five seconds, the display will cease flashing, and store the last displayed value as the new current chamber temperature value.
Continued on next page
Set Temperature °C
43 | P a g e
USER MAINTENANCE (CONTINUED)
Temperature Calibration (Continued)
Use the Up or Down arrows to adjust the flashing current temperature display value until it matches the reference device temperature reading.
Reference Device
Set Temperature °C
Set Temperature °C
After matching the display to the reference device, wait 5 seconds. a. The temperature display will cease flashing and store the corrected chamber display value. b. The incubator will now begin heating or passively cooling in order to reach the set point with the corrected display value.
Allow the incubator to operate undisturbed for at least one 1 hour to stabilize after it has achieved the set point with the
corrected display value. a. Failure to wait until the incubation chamber is fully stabilized will result in an inaccurate reading.
Compare the reference device reading with the incubator temperature display again. a. If the reference device and the incubator temperature display readings are the same or the difference falls within the range of your protocol, the incubator is now calibrated for temperature. b. See the next step if the readings fail to match or fall outside of your protocol range.
Continued on next page
44 | P a g e
Adjusting to Set Point
Set Temperature °C
Reference Device
Set Temperature °C
USER MAINTENANCE (CONTINUED)
Temperature Calibration (Continued)
If a difference still falls outside the acceptable range of your protocol, repeat steps 3 – 7 up to two more times.
Three calibration attempts may be required to successfully calibrate units that are more than ±2°C out of calibration.
Reference Device
Set Temperature ° C
If the temperature readings of the incubator and the reference device still fall outside your protocol after three calibration attempts, contact your
distributor or Technical Support for assistance.
End of procedure
45 | P a g e
USER MAINTENANCE (CONTINUED)
C
ALIBRATE THE
CO
2
D
ISPLAY
Note: Performing a CO
2
display calibration requires a gas reference device. Please see the
Reference Sensor Devices entry on page 10 for the device requirements.
CO
2
calibrations are performed to match the incubator CO
2
display to the actual gas concentration in the incubation chamber. The actual concentration is supplied by a calibrated reference sensor device. Calibrations compensate for drifts in the unit microprocessor controller, as well as those caused by the natural material evolution of the IR CO
2
sensor when continually exposed to a heated and humidified atmosphere with elevated CO
2
concentrations. Calibrate as often as required by your laboratory or production protocol, or regulatory compliance schedule.
CO
2
Supply
The incubator must be powered, the CO
2 set point set, and the chamber supplied with CO
2
for at least two hours prior to the calibration.
Temperature
Temperature drives gas diffusion in the chamber. CO
2
calibrations must be performed with the chamber fully heated and stable at your operational temperature set point. A CO
2
display calibration may be performed during a temperature calibration as long as the chamber door is
not opened during either procedure.
Humidity
Because humidity impacts CO
2 concentration through its influence on temperature stability and uniformity, the CO
2
display should be calibrated with the chamber humidified.
Probes
Connect a digital CO
2
analyzer sample tube to the sample port, located to the right of the control panel.
Stability
Figure 21: CO
2
Sample Port
For best results, allow the unit to operate undisturbed for 12 hours supplied to achieve temperature and RH stability (for example, overnight). A continual CO
2
supply stream may be
introduced a minimum of 2 hours prior to performing the calibration, with the incubator otherwise undisturbed. If the chamber door is opened during the calibration, the incubator must restabilize before continuing with the procedure.
Prior to a calibration, the chamber must operate at its CO
2
set point for at least 1 hour with no
fluctuations of ±0.1% or greater in order to be considered stabilized. Failure to wait for stabilization will result in an inaccurate calibration and incubator display reading.
Continued on next page
46 | P a g e
USER MAINTENANCE (CONTINUED)
Calibrate the CO
2
Display
Once the incubation chamber has stabilized with no fluctuations of 0.1% or greater, compare the gas reference device and chamber CO
2
display readings. a. If the readings are the same, or a difference between the two
(2) falls within the acceptable range of your protocol, the display is accurately showing the chamber CO
2
concentration.
The CO
2
calibration procedure is now complete. b. If there is a difference between the two readings that falls outside the acceptable range of your protocol see the next step.
A display calibration adjustment must be entered to match the incubator
CO
2
display to the reference device.
Reference Device
Set CO
2
%
Set CO
2
%
Reference Device
Set CO
2
%
Place the display in its CO
2
calibration mode. a. Press and hold both the UP and DOWN Set CO
2
arrow buttons simultaneously for approximately 5 seconds. b. Release the buttons when the display shows the letters “CO”.
The display will begin flashing the current CO
2
display value.
Note: If an arrow button is not pressed for 5 seconds, the display will cease flashing, and store the last displayed value as the new current chamber CO
2
value.
Procedure continued on next page
Set CO
2
%
Flashing Display Value
47 | P a g e
USER MAINTENANCE (CONTINUED)
Calibrate the CO
2
Display (Continued)
Use the Up or Down arrows to adjust the current CO
2
display value until it matches the reference device CO
2
reading.
Reference Device
Set CO
2
%
Set CO
2
%
After matching the display to the reference device, wait 5 seconds. a. The display will cease flashing and store the corrected display value. b. The incubator will begin injecting CO
2
or allow the current gas concentration to decay in order to achieve the set point with the corrected display value.
Allow the incubator to operate undisturbed for at least 1 hour undisturbed to stabilize after it has achieved the CO
2
set point
with the corrected display value. a. Failure to wait until the unit is fully stabilized will result in an inaccurate reading and calibration.
Compare the reference device reading with the incubator CO
2
display again. a. If the reference device and the CO
2
display readings are the same or the difference now falls within the range of your protocol, the incubator is now calibrated for CO
2
. a. See next step if the difference still falls outside your protocol range.
Procedure continued on next page
Adjusting to Set Point
Set CO
2
%
Reference Device
Set CO
2
%
48 | P a g e
USER MAINTENANCE (CONTINUED)
CO
2
Calibration (Continued)
Repeat steps 3 – 7 up to two more times if a difference that still falls outside your protocol range.
Three calibration attempts may be required to successfully calibrate units that are more than ±2% out of calibration.
Reference Device
Set CO2 %
If the CO
2
readings of the display and the reference device still fall outside your protocol after three calibration attempts, contact your
distributor or Technical Support for assistance.
End of procedure
49 | P a g e
USER MAINTENANCE (CONTINUED)
A
NODE AND
W
ATER
Q
UALITY
The incubator is provided with a replaceable metal anode located in a threaded port near the waterjacket fill port on the back of the unit. The anode dissolves when in contact with the mineral salts and dissolved gasses found in tap water. This helps to protect the liner of the water jacket from corrosion well as well as scaling and other mineral deposits. When 50% of the anode has eroded away a new anode must be installed (Part Number 0260500).
Tap water with a hardness of more than 30 parts per million or 1.5 grains per gallon will require
assessment of the anode on a yearly basis. Dissolved gasses in tap water used in the water jacket should never exceed more than 120 parts per million or 7 grains per gallon. The pH of the water should be between 6.0 and 8.5.
The manufacturer recommends the use of distilled water whenever feasible. Never use deionized water for any application in the incubator.
Figure 23: Anode Installed,
Back of Unit
Figure 22: Water Anode,
Part Number 0260500
50 | P a g e
UNIT SPECIFICATIONS
The SCO6WE Incubator is a 110 – 120 volt unit. Please refer to the incubator data plate for individual electrical specifications.
Technical data specified applies to units with standard equipment at an ambient temperature of
25°C and a voltage fluctuation of ±10%. The temperatures specified are determined in accordance to factory standard following DIN 12880 respecting the recommended wall clearances of 10% of the height, width, and depth of the inner chamber. All indications are average values, typical for units produced in the series. We reserve the right to alter technical specifications at all times.
W
EIGHT
Shipping
286lbs / 130kg
D
IMENSIONS
By inches
Unit
260.0lbs / 117.9kg
Exterior W × D × H
26.0 x 26.3 x 40.3
By millimeters
Exterior W × D × H
661 x 668 x 1024
Access Port All Units
Diameter
1.0 inch (28.5mm)
Interior W × D × H
20.3 x 19.8 x 25.3
Interior W × D × H
516 x 503 x 643
51 | P a g e
UNIT SPECIFICATIONS (CONTINUED)
M
AX
S
HELVES
F
OR
U
NIT
Shelves
8
C
APACITY
Cubic Feet
5.9
CO
2
Range
0 – 20%
T
EMPERATURE
Range
Ambient +5ºC – 60ºC
P
OWER
Model
SCO6WE
Accuracy
± 0.1%
Uniformity
± 0.2ºC at 37ºC
AC Voltage
110 – 120
Liters
166.0
Recovery Time
Less than 5 minutes
Stability
± 0.1°C @ 37°C
Amperage
5.0
Frequency
50/60 Hz
52 | P a g e
PARTS LIST
Part Part Number
0260500
Anode, Water Jacket
7750514
Access Port Stopper, Size 6
5121514
Air Duct, Chamber Ceiling
PN 9710500
CO
2
Gas Tubing Kit with In-
Line HEPA Filter
5800529
Copper Token,
Humidification Pan
7750513
Fill Port Stopper, Water
Jacket
3300516
Fuse T10A 250V 5X20mm Shelf
Part
Gas Line HEPA Filter
Humidification Pan
Leveling Foot
Power Cord 115 volt 15 Amp,9ft 5 in (2.89m) NEMA
5-15P
Shelf Slides
Shelf Standards
Part Number
2800525
PN 995-00015
2700506
1800510
5121028
5170646
5121777
53 | P a g e
PARTS LIST (CONTINUED)
O
RDERING
P
ARTS AND
C
ONSUMABLES
If you have the Part Number for an item, you may order it directly from Sheldon Manufacturing by calling 1-800-322-4897 extension 3. If you are not certain that you have the correct Part Number, or if you need that specific item, please contact Sheldon Technical Support for help at 1-800-322-4897 extension 4 or (503) 640-3000. Please have the model number and serial number of the incubator ready, as Tech Support will need this information to match your unit with its correct part.
A
CCESSORIES
The following accessories are available for the SCO6WE
CO
2
Cylinder Regulator, Dual Stage
For use with a gas supply cylinder (tank).
Part Number 7150509
Copper Shelf Assembly
Three copper shelves, including six copper shelf slides. Copper is known to have antimicrobial properties.
Part Number: 9750582 complete assembly described above.
PN 5820504 Individual Shelf PN 5820505 Individual Slide
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55 | P a g e
P.O. Box 627
Cornelius, OR 97113
USA [email protected] sheldonmanufacturing.com
1-800-322-4897
(503) 640-3000
FAX: 503 640-1366
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