User Manual for PROGRES GRYPHAX® software
PROGRES GRYPHAX software
JENOPTIK l Healthcare & Industry
JENOPTIK Optical Systems GmbH
Göschwitzer Straße 25
07745 Jena, Germany
Dear user,
Please read the instructions in this manual carefully before starting to operate the PROGRES GRYPHAX®
camera and the software PROGRES GRYPHAX®. By observing the advice in this manual, you can make
optimum use from the functions, and you can avoid damages or injuries resulting from operating errors.
Editorial deadline: September 2017
Documentation number: 001001
JENOPTIK | Healthcare & Industry
JENOPTIK Optical Systems GmbH
Goeschwitzer Strasse 25
07745 Jena
Germany
Phone: +49 3641 65-3083
Fax: +49 3641 65-2144
E-mail: progres@jenoptik.com
www.jenoptik.com/progres-gryphax
Revision state
Date
September 2017
Release
001
Revision
001
Remarks
This manual or parts of this manual must not be reproduced in any form (print, photocopy, micro film or
any other procedure) without a prior written permission of Jenoptik, nor must contents be used,
reproduced, processed or distributed by means of electronic systems. This manual was produced with
the appropriate care. No liability will be accepted for damages resulting from the non-compliance with
the advice contained herein. We reserve the right to make improvements and changes to the device and
this manual at any time without prior notice.
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Disclaimer:
This software manual was produced with due care. We reserve the right to make improvements and
changes to the device and to this instruction manual at any time without prior notice. No liability will be
assumed for damages or injuries arising from noncompliance with the information contained herein, or
in any further applicable document!
Property rights:
PROGRES GRYPHAX® is a Jenoptik trademark registered for the European Union and the United States
of America. All other brand names, trademarks and pictures of products shown or mentioned in this
instruction manual are property of their lawful owners and are used here only for the purpose of
description.
Scope:
This instruction manual applies for the operation of the PROGRES GRYPHAX® application software.
Contents:
This software manual contains all necessary information on capturing and processing microscope images
with the PROGRES GRYPHAX® application software. The manual does not contain any safety advice and
installation instructions for the PROGRES GRYPHAX® software nor any repair instructions.
Further applicable documents:
• “PROGRES GRYPHAX® microscope cameras – safety and instruction manual“
The instruction manual contains all necessary information on the safe installation and operation of the
cameras, on storage and transportation. This document is included in the delivery of your camera.
Before you start to operate the software, please read the safety advice carefully and observe the
installation instructions.
Technical data:
The technical data of your PROGRES GRYPHAX® camera can be found on the installation USB stick and
on our web site:
https://www.jenoptik.com/progres-gryphax
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Table of Content
1.
2.
3.
4.
Graphical User Interface (GUI) ......................................................................................................... 9
1.1
Overview of GRYPHAX interface ............................................................................................ 10
1.2
Title bar ................................................................................................................................. 10
1.3
REC bar.................................................................................................................................. 11
1.4
Tool bar & Help bar ............................................................................................................... 12
1.5
Gallery ................................................................................................................................... 13
1.6
Treeview ................................................................................................................................ 14
1.7
Image window ...................................................................................................................... 14
1.8
Header .................................................................................................................................. 15
1.9
Status bar .............................................................................................................................. 15
1.10
Video tutorial......................................................................................................................... 15
Preferences ................................................................................................................................... 16
2.1
Enter Preferences ................................................................................................................... 17
2.2
General.................................................................................................................................. 18
2.3
Camera .................................................................................................................................. 19
2.4
Storage Options..................................................................................................................... 20
2.5
Device Configuration ............................................................................................................. 21
2.5.1
Create ................................................................................................................................ 22
2.5.2
Remove ............................................................................................................................. 22
2.5.3
Create custom objectives ................................................................................................... 23
2.5.4
Remove custom objectives ................................................................................................. 23
2.5.5
Create device ..................................................................................................................... 23
2.6
Status Bar .............................................................................................................................. 24
2.7
Elements of Style ................................................................................................................... 24
2.8
Additional Services................................................................................................................. 25
2.9
Transfer software settings ...................................................................................................... 25
User Profiles .................................................................................................................................. 26
3.1
Location ................................................................................................................................ 26
3.2
Create ................................................................................................................................... 27
3.3
Load ...................................................................................................................................... 27
3.4
Delete .................................................................................................................................... 27
3.5
Transfer software settings ...................................................................................................... 28
Gallery & Gallery bar...................................................................................................................... 29
4.1
Media file structure................................................................................................................ 31
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4.2
5.
Gallery bar ............................................................................................................................. 32
Image Analysis function................................................................................................................ 34
5.1
Preparation ............................................................................................................................ 35
5.2
Start image transfer ............................................................................................................... 35
6.
Side by Side ................................................................................................................................... 36
6.1
Start Side by Side mode ......................................................................................................... 36
6.2
Change images ...................................................................................................................... 37
6.3
Leave Side by Side mode ....................................................................................................... 37
7.
Tree view tool ............................................................................................................................... 38
7.1
Change storage folder ........................................................................................................... 39
7.2
Create storage folder ............................................................................................................. 39
8.
Exposure and Histogram ............................................................................................................... 42
8.1
Open Exposure ...................................................................................................................... 43
8.2
Maximum exposure time ....................................................................................................... 43
8.3
Set Grey Balance.................................................................................................................... 44
8.4
Set Black Point ....................................................................................................................... 44
8.5
Set White Point...................................................................................................................... 44
8.4
Open Histogram .................................................................................................................... 44
9.
Image Enhancement tools – Color & FX ........................................................................................ 46
9.1
Open Color tool..................................................................................................................... 47
9.2
Open FX tool ......................................................................................................................... 48
10.
Measurement tool ..................................................................................................................... 50
10.1
Start Measurement tool ......................................................................................................... 51
10.2
Pre-define settings ................................................................................................................. 51
10.3
Start measuring ..................................................................................................................... 51
10.4
Creation (drawing) of objects ................................................................................................ 52
10.5
Draw a distance (line) & freeform measurement: ................................................................... 52
10.6
Draw a radius & diameter (circle) measurement ..................................................................... 53
10.7
Draw an angle measurement ................................................................................................. 53
10.8
Object selection ..................................................................................................................... 54
10.9
Object multi-selection ............................................................................................................ 54
10.10
Deleting objects ................................................................................................................. 54
10.11
Changing object properties ................................................................................................ 54
11.
11.1
Scale bar tool ............................................................................................................................ 56
Preparations general .............................................................................................................. 57
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11.2
Select unit type and color ...................................................................................................... 57
11.3
Activate scale bar................................................................................................................... 57
11.4
Place scale into image............................................................................................................ 57
11.5
Modify scale bar .................................................................................................................... 58
11.6
Delete scale bar ..................................................................................................................... 58
11.7
Save scale to live .................................................................................................................... 58
11.8
Save scale to recorded images ............................................................................................... 58
12.
Annotations tool ....................................................................................................................... 60
12.1
Start Annotations tool ........................................................................................................... 61
12.2
Pre-define settings ................................................................................................................. 61
12.3
Start & save drawings ............................................................................................................ 61
12.4
Creation (drawing) of objects ................................................................................................ 62
12.4.1
Draw an arrow................................................................................................................... 62
12.4.2
Draw a text field ................................................................................................................ 62
12.5
Object selection ..................................................................................................................... 63
12.5.1
Object multi-selection ........................................................................................................ 63
12.6
Deleting objects ..................................................................................................................... 63
12.7
Changing objects properties .................................................................................................. 63
13.
Report tool ................................................................................................................................ 65
13.1
Start Report tool .................................................................................................................... 66
13.2
Software settings view ........................................................................................................... 67
13.3
Measurement data table........................................................................................................ 67
13.3.1
13.4
14.
Select or delete measurements at data table ...................................................................... 68
Image meta-data view ........................................................................................................... 68
MonoChrome mode .................................................................................................................. 70
14.1
Start MonoChrome mode ...................................................................................................... 71
14.2
Deactivate MonoChrome mode ............................................................................................. 71
15.
Fluorescence tool ...................................................................................................................... 72
15.1
Preparations general .............................................................................................................. 72
15.2
Overview ............................................................................................................................... 73
15.3
Status of filter set................................................................................................................... 74
15.4
Start & initial set-up ............................................................................................................... 75
15.5
Re-record images ................................................................................................................... 76
15.6
Save to Gallery ....................................................................................................................... 77
16.
Panorama tool ........................................................................................................................... 78
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16.1
Preparations .......................................................................................................................... 78
16.2
Start ...................................................................................................................................... 79
16.3
Lost matching ........................................................................................................................ 80
16.4
Manual mode ........................................................................................................................ 80
17.
Z-Stacking tool .......................................................................................................................... 82
17.1
Preparations .......................................................................................................................... 82
17.2
Start ...................................................................................................................................... 83
17.3
Save....................................................................................................................................... 84
18.
18.1
19.
19.1
20.
Grid mode ................................................................................................................................. 85
Start Grid mode ..................................................................................................................... 86
Presentation mode .................................................................................................................... 88
Start Presentation mode ........................................................................................................ 89
Video mode............................................................................................................................... 90
20.1
Start Video mode................................................................................................................... 91
20.2
Replay Video files................................................................................................................... 92
20.3
Change storage format .......................................................................................................... 93
21.
Time-lapse mode ....................................................................................................................... 94
21.1
Start Time-lapse mode ........................................................................................................... 95
21.2
Change storage format .......................................................................................................... 95
21.3
Start Recording ...................................................................................................................... 96
21.4
Replay Time-lapse sequences ................................................................................................. 97
22.
System requirements ................................................................................................................. 98
23.
Technical drawings & interfaces............................................................................................... 100
24.
Keyboard shortcuts.................................................................................................................. 101
25.
TWAIN / DirectX interface........................................................................................................ 102
25.1
Overview of settings window .............................................................................................. 103
25.2
Toolbar ................................................................................................................................ 103
25.3
Camera image parameter .................................................................................................... 105
25.4
Capture / Transfer images .................................................................................................... 107
25.5
Start / Stop Live ................................................................................................................... 107
25.6
Preview window .................................................................................................................. 107
25.7
Preview window tools and options ...................................................................................... 108
26.
USB 3.0 Camera Service Tool ................................................................................................... 110
26.1
Firmware update .................................................................................................................. 111
26.2
Dark blemish search ............................................................................................................. 112
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27.
Service & Support .................................................................................................................... 114
28.
Return address & software updates ......................................................................................... 114
29.
Safety & Operating Instruction for PROGRES GRYPHAX Microscope Cameras .......................... 115
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1. Graphical User Interface (GUI)
Graphical User Interface (GUI) of PROGRES GRYPHAX®
software
General description:
PROGRES GRYPHAX software is a modular and platform independent software for state of the art
microscopy. It contains camera control and image processing to optimize by a wide range of techniques.
The “Graphical User Interface” of the GRYPHAX software is platform independent and delivers identical
GUI and functionalities under Windows, MacOS and Linux.
The interface is divided into three main areas. In the center screen area of the interface is the image
window to display live preview from camera or loaded media files from Gallery.
At the left-hand site are the Gallery tool and the Treeview located. They provides an easy and fast
overview about advance file management and displays all images and media files as thumbnails.
On right-hand site the toolbar provides access to record modes and all image processing enhancements
tools. Additionally, tools to measure and annotate and image acquisition modules like “Panorama”,
“Z-Stack” (EDF) or Multi-Fluorescence are available.
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1.1
Overview of GRYPHAX interface:
1 Title bar
2 REC bar
3 Gallery
1.2
4 Image window
5 Header
6 Tool bar
7 Help bar
8 Treeview
9 Status bar
Title bar:
The title bar of GRYPHAX software contains the menu bar to access all menus you need to manage, edit
or to change views. Additionally, you have quick access to create and change current user profiles.
- Minimize program window
- Maximize program window
- Change program window size to any selected
- Close program window | short cut (alt + F4 / cmd + Q)
File menu:
 Enter software Preferences
 Save copies of selected media files to alternative destinations
 Print selected images from Gallery
 Quit software
View menu:
 Change to Presentation mode
 Activate / deactivate Grid mode overlaid at image window
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Service menu:
 Help – open GRYPHAX FAQ website
 Update Check – starts download page to check for software updates
 Website – open GRYPHAX website
 Log File – open software log file for trouble shooting
 About – displays software information
 Activate “Service Mode” for trouble shooting – saves special tiff image with log file
User Profile:
 Default – reset back the factory “default” software settings
 Last Session – change back to last stored session settings of software
 User Profiles – open list to select or change already created user profiles
 Save As – save current software settings to user profile (op. password protection)
 Delete – selected user profile from list
Also current user profile e.g. “Last Session” will be displayed directly at title bar and updates accordingly.
Note: Double-click on title bar changes between maximizes program window view and reduced program
window view to any selected size.
1.3
REC bar:
The REC bar is the most important tool of the GRYPHAX software. It control all image capture and file save.
The status of the Rec bar buttons are changing accordingly to status of software.
REC button indicates live preview is running, by pressing button images will be captured to Gallery
Live button indicates that no live preview is running and an image from Gallery is displayed instead
Stop button indicates recording / capturing is activated, pressing button stops record / capture process
Magnifier button – activates magnifier glass to observe important image areas
During Magnifier mode, Zoom level of displayed image from image window can be changed by
keyboard key: “+” or “-“ to zoom in or zoom out.
Arrow tool button – to change back to standard mouse pointer “arrow” to select or adjust e.g.
overlays of measurement or annotations
Hand tool button – to activate hand tool during “1:1” view or Zoom. Mouse pointer change to
“hand” and image section can be moved.
“1:1” button indicates that “Fit to Window” is active, pressing button changes to “1:1” view
Fit to window button indicates that “1:1” view is active, pressing button changes to “Fit to Window”
view
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1.4
Tool bar & Help bar:
To open the GRYPHAX tool bar by pressing the arrow
short cut (ctrl / cmd + T)
on right-hand software site or use keyboard
The Toolbar is divided in 4 parts and contains the following sections:

1 Record modes – to change between record modes:
“Single shot”, “Time-lapse” or “Video record”

2 Tools section – to activate / deactivate tools

3 Active tool section – displays activated tool widgets

4 Help section – shows tooltips and help information of tools by
mouse over
Tool status:


Activated tools are highlighted in blue.
Tools which are not access able are “greyed out” by software
automatically and cannot be reached.
Collapse / expand
active tool section.
button on each tool to hide separate widgets on
Reset button
on a tool sets all properties to their initial values. Initial
values are fixed.
To activate or deactivate “Help” section, click on the Help icon
help bar. Active help section is indicated by blue icon.
on
Note: Status and accessibility of tools depending on previously activated
tools. Not all tools and options can be activated at the same time!
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1.5
Gallery:
The “Gallery” of PROGRES GRYPHAX® software enables user to preview
and display all saved media files of media destination folder or from
selected destination path of “Tree view” tool easily inside of GRYPHAX
software.
To open the GRYPHAX Gallery by pressing the below arrow on
left-hand software site or use keyboard short cut (ctrl / cmd + G).
The Gallery consist of two parts. The Gallery preview window (1) and the
Gallery bar (2).
The Gallery windows shows the media files thumbnails from top to
bottom in order of the file time stamp. New recorded images or media
files will be add on top position at Gallery preview window.
To change the media destination folder you can use the Treeview tool
or change folder at the software preferences under storage options.
At the Gallery preview window you can use operation system
typical short cuts (e.g. ctrl + A; shift-key + mouse click) to select
items.
Note: The more images are stored in a folder and are loaded in a Gallery,
the longer it will take to display a single image. To enable an easier
workflow with GRYPHAX, we recommend you to reduce the number of
images in the target folder by changing the target folder from time to
time, by deleting some images or by copying them to another folder.
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1.6
Treeview:
The Treeview tool is located on the left software side next to
the Gallery. It can be used with and without opened Gallery.
Treeview deliver two different sizes: “Narrow” or “Wide”
view to display the media target tree.
To open, expand or close of the GRYPHAX Treeview tool
press the top left arrow
on the left-hand software side or
use the keyboard short cut (ctrl / cmd + D).
Note: After first software start the Treeview tool is starting
and displaying the default destination folder of operating
system “own Pictures” from current user.
Alternatively, the Treeview is automatically displaying and
using the last used destination folder from last software
session or from activated user profile settings.
The media destination folder of Treeview is always
synchronized with settings of software preferences under
section storage options.
1.7
Image window:
The image window is the main software area. All other tools can be hidden to have full focus at live
preview from active camera or at loaded images from Gallery.
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Additionally, a small overview window will be displayed over the image at “1:1” view. The currently
displayed part of image is marked as rectangle. The position (ROI) can be moved by mouse operation.
Also size and position of overview window can be changed by mouse operation.
Note: A full screen view without any software GUI can be activated by “Presentation” mode under
menu: “View” at the title bar or by keyboard short cut (F11).
1.8
Header:
The header section of program window is displaying the following information from image window.
Frame Rate – Indicates the current frame rate in frames per second (fps) delivered by the active camera.
Zoom factor – Indicates the current zoom level (in %) of displayed image from image window.
1.9
Status bar:
At the status bar of GRYPHAX software contains powerful options to operate with GRYPHAX software.
The status bar is divided in 4 sections (text field, device configuration, calibrated objectives and scale).
Text field – to enter a comment or notification which is directly saved
to the status bar of recorded images.
User can fast change between created device configurations
and calibrated microscope objectives to apply correct
measurement calibrations and scale for the preview image
from camera.
Additionally, the scale bar is displayed according selected
objective.
Note: Loaded images from Gallery with included meta-data calibration are displaying the correct values
and scale automatically and independent from selected device or magnification of status bar.
1.10
Video tutorial:
Press the link to watch the video tutorial for Graphical User Interface (GUI) of GRYPHAX software.
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2. Preferences
Preferences of PROGRES GRYPHAX® software
General description:
PROGRES GRYPHAX software is a modular and platform independent software for state of the art
microscopy. It contains camera control and image processing to optimize by a wide range of techniques.
To work versatile with favorite equipment, user can use the GRYPHAX Preferences to create own device
configurations which can optionally transferred to other computers and software platforms.
The “Preferences” menu of GRYPHAX software is located at the title bar and contains all major software
and camera settings for initial software setup and to generate individual device configuration.
Opened preferences during device calibration:
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2.1
Enter Preferences:
To enter software Preferences go to the program title bar under menu File / Preferences. A submenu will
be displayed to directly enter specific preferences section.
For the initial software start, we strongly recommend to begin with top first preferences entry “General”
and follow up all sections step by step.
File menu and submenu to enter software
Preferences.
After click on option e.g. “General” a
separate “Preferences” window will be
opened. The window is separated into
two areas:
To navigate through the preferences
sections click to the corresponding
option from column on the left window
side (1).
To change options according your
needs edit on right window section (2).
To apply any changes to software
settings, leave preferences window by
“OK” button!
To leave preferences without applying
any changes press “Cancel” button.
Note: Some options depending on
camera type. Disabled options are
greyed out and can’t be reached.
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2.2
General:
At “General” menu all global software settings can be adjusted. Initial language is assumed from system
language. All other settings are pre-defined by software default values.
Language
To change language select one of the
available software languages from drop
down list. Entire software GUI will
change language after software restart.
Minimize Gain
Option “Minimize Gain” is activated as
default. Function increase image quality
by reducing noise level for recorded
images by minimize gain value.
Deactivate this function if you want to
record images with gain factor from
live preview.
Color space
To switch between “sRGB” and
AdobeRGB color space to enhance
color reproduction on corresponding
monitor. AdobeRGB currently disabled.
Scaling
To adjust scaling factor of software
interface (GUI) in correlation to high
resolution screens (e.g. UltraHD/4K/5K).
Select according scaling factor from
drop down list. Entire software GUI will
change scaling after software restart.
Magnifier
To adjust the zoom factor of detail window for magnifier tool.
The factor can be chosen in steps from 100% to 300%.
Select according zoom factor from drop down list.
Gallery
To adjust the icon sizes of preview thumbnails in gallery window.
The size can be chosen between small preview and big preview thumbnails.
Select according size option from drop down list.
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2.3
Camera:
At “Camera” menu all camera related settings can be adjusted independently from configured devices.
Camera
To select connected camera from drop
down list. Camera will be activated
immediately. Live preview is starting at
image window in background.
Note: Connecting multiple cameras from
same type will be displayed by a suffix
number at name to distinguish from each
other.
Black Shading
The Black Shading function minimizes noise by subtracting a black reference image from recorded images.
It is recommended to create black shading correction especially for long exposure images e.g.
Fluorescence or dark field applications.
Before create a black shading reference file, remove camera from microscope and close the sensor by
the protection cap. Take care that no light falls into the sensor, otherwise the creation will be aborted.
Press the icon
successfully finished.
“Calibrate Black Shading” to start and wait until the process is
During creation the calculated remaining time and
status will be displayed.
After successful creation the file date will be displayed on preferences window.
Black Shading correction can be switch on or off by selecting of corresponding radio buttons
On the “Help” button
you will find detailed information to create shading reference file.
Note: Black shading creation can take up to 30 minutes depending on camera type.
Cooling
Option to activate camera cooling to stabilize camera operation in warmer environmental condition.
Note: This option is available for cameras with active cooling function only.
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.
2.4
Storage Options:
At “Storage Options” menu all related options for image format and storage can be adjusted.
Auto Save
Option Auto Save is activated as
default. With the storage option
“Auto save” all recorded media
files will be saved with an
automatic given naming
structure. Manual save can be
used by deactivate Auto save
function.
Image Format can be select from
drop down list. The following
formats are available: bmp, jpg,
tif and png
At option Image Quality user can select quality level and bit depth of according selected image format.
Option File Name represents the image name prefix for auto saved images.
Create, select or delete file name to any individual prefix at drop down menu.
Video Format can be select from drop down list. The following formats are available: wmv, webm and mp4
depending on operating system.
Capturing
By option Capturing user can adjust preview duration time of recorded images after capture.
The time can be chosen in steps from 1 second till infinite by drop down list.
If option “Show image after capture” is deactivated, live image immediately restarts after
image capture.
Destination Folder
To change media file destination folder where to save recordings. Click on “Browse” button
and a separate window will be opened. Select correspond folder by browse folder structure of hard
drive. Confirm new destination folder by “Select Folder” button.
After change of destination folder, the Gallery will display all media files of new selected folder. All new
captured images and media files will be save to the selected folder. Furthermore, the media destination
folder of Tree view tool is synchronized with the software preferences.
Additionally, a separate media files destination folder for cloud services can be predefined.
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2.5
Device Configuration:
At menu “Device Configuration” user can create individual device sets to enhance user’s daily work-flow
by changing between different cameras or workstations within seconds!
Devices
To create, select, rename or delete device
configurations at drop down menu.
Camera Options
To select a connected camera from list.
Change image orientation according
camera mounting from drop down list in
steps of 90° (degree).
Resolution
Choose available aspect ratio from list.
Resolutions for “Live” & “Capture” will be
displayed accordingly.
Define camera resolution separately for live
preview and captured images.
Activate “Fast record” option for fast image
capture. Not supported for scanning modes.
Colour Temperature
To select the temperature for colour correction according light source at drop down list to reach best
colour reproduction. With option “Auto” software automatically choose best fitting colour correction
according your light source after grey balance adjustment.
White Shading
Before create a white shading reference file, remove specimen from microscope and activate the light
source. Take care that no dust or specimen is in the light path, otherwise the white reference file will
correct temporary objects.
Press the icon
successfully finished.
“Calibrate White Shading” to start and wait until the process is
After successful creation the file date will be displayed on preferences window.
White Shading correction can be switch on or off by selecting of corresponding radio buttons:
On the “Help” button
user will find detailed information to create shading reference file.
Note: White shading reference will be connected to the currently used objective from microscope. By
change of objective at microscope a new white shading reference must be created!
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Measurement (calibration)
To use Measurement tool you have to calibrate your microscope objectives in advance! Otherwise the
Measurement tool cannot be activated and used.
To calibrate the microscope you can use the PROGRES GRYPHAX®
calibration slide (order number: 563683).
2.5.1
Create:
To create measurement calibration please oriented the object micrometer
horizontally on the microscope stage. Select objective type from drop down
list according to the currently used objective from microscope e.g. 10x.
Press “Calibrate” button
line onto live preview from image window.
to start drawing a reference
Start drawing by left-hand mouse click on first reference point and
release. Move mouse to reference endpoint confirm position by click
and release again. While drawing a detail window will appear to find
exact positions.
Reference line will be drawn as overlay onto the image window and
the calibration value window will appear.
Enter reference length into the calibration value
window. Choose the correct unit from drop down list and finally confirm by
button “Finish”.
After successful objective calibration the status for each objective
from list is displayed by “
” in green.
Note: If an objective is not on the list, user can create individual custom objectives as well.
2.5.2
Remove:
To remove created calibration from list, select corresponding objective type and use the drop down
menu entry “Remove Calibrations”
. Only calibration information will be deleted.
After remove, calibration status of objective will be displayed as not calibrated “
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”.
2.5.3
Create custom objectives:
To create a custom objective use the drop down menu entry
from drop down list.
Enter custom objective name at input field and start objective calibration
with button “Calibrate”
.
After successfully calibration the custom objective is added to the objective drop down list of
measurement and the status for objective from list is displayed by “
” in green. The new
custom objective will be available at magnification menu of status bar.
2.5.4
Remove custom objectives:
To remove custom objective calibration from list, select corresponding objective type and use the drop
down menu entry “Remove Objective Calibrations”. Custom objective entry at list and calibration
information will be deleted.
Video tutorial:
Press the link to watch the video tutorial how to calibrate the microscope objectives.
2.5.5
Create device:
(1)
To start with “device” creation follow up the
given structure step by step by choose option
“New” to enter individual “device name” at first.
(2)
Select a camera from camera list and set all
relevant parameters from “Device Configuration”
page and calibtrate all objectives of microscope.
(3)
To save device configuration and confirm all changes from preferences press button “OK”
(4)
After succesfull device configuration users are able to switch easily between created devices and
calibrated magnifications at the “Status Bar” of GRYPHAX software.
Note: Remove device configurations by option “Delete” will lost all settings and calibrations of those!
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.
2.6
Status Bar:
At “Status Bar” menu all related options for status bar and scale bar can be defined. In addition a “status
bar“ can be added at the bottom of captured images to display significant information like: scale, device
name, used magnification and notification.
Settings
Decide if status bar should be added to the
captured images by radio buttons On / Off.
Check mark all information which has to be
displayed on added status bar.
Description
Choose the appearance of the added status
bar between:
(a) “White text on Black background” or
(b) “Black text on White background”
Note: The drag and drop scale bar from status bar will be appear within these setting overlaid on the
image window.
Scale bar
To adjust the scale bar unit of status bar from software and at added status bar to
captured images. Adjust between units: “Metric” or “Imperial/US”.
2.7
Elements of Style:
At “Style” menu general options for software style and colors can be adjusted.
Grid
Lines colour for Grid view can be predefined by colors of drop down list.
Note: Line pitch will be always restored from
last defined line pitch of Grid mode use.
For more detailed information, please have a look to the separate manual section for “Grid mode”.
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2.8
Additional Services:
At “Additional Services” menu the image analyses settings can be pre-defined. The “Image Analysis Option”
enables user to open image from GRYHAX Gallery directly at 3rd party “analysis” applications.
Define:
To select an external
application press the button
and
choose appropriate
application e.g ImageJ from
your computer.
Selected application file
path will be displayed at
preferences window.
For more detailed information, please have a look to the separate manual section for “Image Analysis
function”.
2.9
Transfer software settings:
Software settings or “user profiles” can be transferred to other computer (platform independent) to
work with same settings or devices! User can copy and paste the software settings file from current
computer and transfer to others.
The current software settings are stored at settings file: “lastsession.ini” located under following system
folder depending on used operating system:



Windows OS: „C:\ProgramData\Jenoptik\...”
MacOS: „MacHD\Library\Application Support\Jenoptik\...”
Linux OS: „/var/lib/Jenoptik/...”
Please note: Deleting or rename of settings files will lost all software settings and calibrations!
Please handle with care! For details about transfere software settings, please have a look to the separate
manual section: “Transfer software settings” at user profile tool.
Video tutorial:
Press the link to watch the video tutorial for Preferences of GRYPHAX software.
Limitations:
For correct software operation it is mandatory that user has permission of “full control” for software
settings location (system folder of OS) and settings files.
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3. User Profiles
User Profile tool of PROGRES GRYPHAX® software
General description:
The “User Profile tool” of PROGRES GRYPHAX® software enables user to create user specific software
settings profiles to get reproduce able results by identical software and camera settings.
The currently active software settings can be saved in a user profile (preference file). These user profiles
can be loaded and applied. Furthermore, the user profiles can be transferred to other computer or
platforms. (Windows, MacOS and Linux)
Overview of User Profile menu:
3.1
Location:
The User Profile menu is located at the title bar of GRYPHAX software. User has quick access to create or
load user profiles. Additionally, the current user profile e.g. “Last Session” is prominently displayed at
title bar.
User Profile menu provides the following options:





Default – Caution! Reset all back to factory “default” software settings!
Last Session – change back to last stored session settings of software
User Profiles – open profile list to load already created user profiles
Save As – save current software settings to user profile (optional password protection)
Delete – to delete selected user profile from list
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3.2
Create:
To create a unique user profile, pre-define all software
setting as required in advance!
After define go to user profile menu and click on option
“Save As” to save all current settings to file.
A new window to set name of profile will be opened.
Enter profile name and proceed with “OK”.
After confirm of profile name user can optionally
protect the user profile by password. (Recommend)
Enter an individual password and confirm with “OK”
button.
In case that no password protection is needed press
“Cancel” button to create profile without password.
Note: Please remember, or make a note of the given password. It can’t be changed afterwards!
Protected user profiles are indicated by a red padlock at the name.
Blue padlock at name indicates an unprotected user profile.
3.3
Load:
To load a user profile go to user profile menu and click on
option “User Profiles” to select profile from list. After loading
the profile all software settings from file are loaded as current.
If user change user profile or change back to “Last Session”
settings all intermediate changes are not saved and will be lost!
Change profile settings:
To change values or setting from existing user profiles. Load profile first and make any changes.
Afterwards, go to user profile menu and click on option “Save As” and enter profile name which has to
be changed. By press “OK” button all changed software settings will be overwritten from user profile.
Note: protected user profiles can’t be changed afterwards. Create alternatively a new protected profile.
3.4
Delete:
To delete a user profile load the corresponding profile from list. Afterwards go to user profile menu and
click on option “Delete” to remove profile from list. Profile “Last Session” can’t be deleted.
Note: To delete password protected user profiles you have to enter correct password to proceed!
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3.5
Transfer software settings:
Software settings or “user profiles” can be transferred to other computer (platform independent) to work
with same settings or devices! User can copy and paste the software settings files from current computer
and transfer to others. User profile file name is similar to given name with extension *.ini.
The current software settings called “Last Session” are stored at settings file: “lastsession.ini” located under
following folder depending on used operating system:

Windows OS: „C:\ProgramData\Jenoptik\PROGRES GRYPHAX\profiles\...”

MacOS: „MacHD\Library\Application Support\Jenoptik\PROGRES GRYPHAX\profiles\...”

Linux OS: „/var/lib/Jenoptik/PROGRES GRYPHAX/profiles/...”
Important note: Deleting or rename of settings files will lost all software settings and calibrations!
Please handle with care!
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4. Gallery & Gallery bar
Gallery tool of PROGRES GRYPHAX® software
The “Gallery” of PROGRES GRYPHAX® software enables user to preview and display all saved media files
of media destination folder or from selected destination path of “Tree view” tool easily inside of
GRYPHAX software.
General description:
The Gallery is located on the left software side next to the Tree view tool. It
can be used with and without opened Tree view tool. The Gallery consist of
two parts. The Gallery preview window and the Gallery bar.
The Gallery preview window shows thumbnails from the media files of
selected destination folder. The Gallery bar contains additional options for
selected items from Gallery.
To open or close of the Gallery tool press the bottom arrow
software side or use the keyboard short cut (ctrl / cmd + G).
on the left
The Gallery shows the thumbnails in order of the file time stamp from top to
bottom. New recorded images / media files will be add on top position of
Gallery preview window.
After first software start the Gallery tool is collapsed and will displaying the
media files of default destination folder of operating system: “own Pictures”
from current user after opening.
To change the media destination folder you can use the Tree view tool or
change folder at the software preferences under storage options.
At the Gallery preview window you can use operation system typical short
cuts (e.g. ctrl + A; shift-key + mouse click) to select items.
Note: The more images are stored in a folder and are loaded in a Gallery, the
longer it will take to display a single image. To enable an easier workflow
with GRYPHAX, we recommend you to reduce the number of images in the
target folder by changing the target folder from time to time, by deleting
some images or by copying them to another folder.
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Change thumbnail view
The thumbnail view can be displayed by two different options:
 A large thumbnail preview as default setting
 Or a small list preview
To change thumbnail preview of Gallery window, open Preferences /
General / Gallery
Navigation:
Vertically scrolling via mouse wheel or scroll bars.
media file thumbnails exceeds available space of gallery window.
Show complete file name:
To show the complete name of media file, you can use the tooltip of
thumbnail.
Hold the mouse pointer over according media thumbnail at gallery
window until complete file name will be displayed (hover).
View destination path:
To quickly display the complete destination path of
each folder you can use the tooltip at Tree view tool.
Hold the mouse pointer over according folder of tree
view until complete path will be displayed (hover).
To display the destination path of saved images
from Gallery without Tree view tool you can
use the path information of “Report” widget.
Hold the mouse pointer over Path of Report
widget until complete path will be displayed
(hover).
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Scroll bars appears if number of
4.1
Media file structure:
With the storage option “Auto save” all recorded media files will be saved with an automatic given
naming structure. The file name contains the following: prefix, date, time and the file extension
separated by an underline “_”. This structure cannot changed by user.
As example: “Image_20170419_094644439.jpg”
prefix_date_time.file extension
The prefix for single shot images is “Image” as default setting and can be changed by user under
Preferences / Storage Options / File Name (default = “Image”)
Depending on media type the file “prefix” will be automatically given by software as follows:






For Panorama images = Panorama_date_time.file extension
For Z-Stacking images = ZStack_date_time.file extension
For merge Fluorescence images = Multifluorescence_date_time.file extension
For Fluorescence filter set images = Filterset_date_time.file extension
For Video files = Video_date_time.file extension
For Service images = Service_date_time.file extension
With this structure user can easily distinguish between different images file types.
The date and time structure: YYYYMMDD_ hhmmss (ms)(ms)(ms) is the given date stamp automatically
during file saving by software.







Y = Year
M = Month
D = Day
h = hour
m = minutes
s = seconds
ms = milliseconds
The file extension depends on the used storage file format (*.jpg ; *.bmp ; *.tif ; *.png). Storage format
can be changed under Preferences / Storage Options / Image Format.
Rename files:
To rename media files, please make a mouse click on the according
thumbnail to mark and click a second time to the displayed name text under
the thumbnail to activate renaming. You can now overwrite the current
image name by a new text containing letters or numbers. You can leave the
overwrite mode by clicking on another image or by press enter key.
Note: The file extension will be not changed at Gallery.
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Status of selection:
To display a “recorded” item from Gallery on GRYPHAX image window
double-click to the according thumbnail.
The thumbnail in the Gallery change their display state. Thumbnail will
be bordered by a blue line and file name background will be colored in
blue.
Select item by left-mouse click to the thumbnail within the gallery to
remove or execute any option from Gallery bar.
The thumbnail in the Gallery change their display state. Thumbnail file
name background will be colored in blue.
Note: To select multiple items you can use operation systems typical
short cuts (e.g. ctrl + A; shift-key + mouse click).
4.2
Gallery bar:
The Gallery bar is located at the bottom of Gallery preview window
and contains the following options:
E-Mail – to send selected items by E-Mail (currently deactivated)
Print – to print selected items (keyboard shortcut ctrl / cmd + P)
Cloud – to copy selected items to cloud storage folder (Cloud folder can be changed at preferences)
Analyze – to analyze selected items from Gallery in external applications (Choose App at preferences)
Trash – to move selected items from Gallery (destination folder) to system trash
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Delete media files:
To delete media files from Gallery select the according items and use the trash
keyboard key “DEL”.
of Gallery bar or use the
You will need to accept the prompt “Are
you sure to move selected image/ images
to system trash?” with “Yes“.
Note: The media files will be deleted from the destination folder and are moved to operating system trash!
Limitation:



Gallery is not visible during “Presentation” view.
Gallery cannot show external media files.
Gallery functions are limited during Multi-Fluorescence mode
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5.
Image Analysis function
“Image Analysis” function of PROGRES GRYPHAX®
software
The “Image analysis function” of PROGRES GRYPHAX® software enables user to send saved media files
from Gallery to external 3rd party imaging software tools directly from GRYPHAX software. So user are
able to process images further or use specific methods from analysis software.
Overview:
Image from GRYPHAX directly transferred to 3rd Party software e.g. “ImagePro Premier” (Media Cybernetics)
General description:
The “Image analysis function” is part of the Gallery bar. Located on the left
software side at the bottom of the Gallery.
The Gallery preview window shows thumbnails from the media files of
selected destination folder. The Gallery bar contains additional options for
selected items from Gallery.
To open or close of the Gallery tool press the bottom arrow
software side or use the keyboard short cut (ctrl / cmd + G).
on the left
Note: The Gallery bar will be active after selection of one media thumbnail.
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5.1
Preparation:
To use the “Image analysis function” open
Preferences and navigate to the section:
“Additional Services” and pre-define the
Application you want to transfer the images
from GRYPHAX software.
Press the bottom “Please choose your
application”
and a separate file selection windows will be
opened to search and select the appropriate
3rd party software. (e.g. ImageJ or ImagePro)
5.2
Start image transfer:
To activate the image transfer to 3rd party software tool, select the
according image file thumbnail from GRYPHAX Gallery by left-hand
mouse click and press the “Image analysis” button
from Gallery bar.
The analysis function immediately opening the pre-selected 3rd party
software and transfer the selected image.
Note: Multiple items cannot by transferred by “Image analysis” function.
Video tutorial:
Press the link to watch the video tutorial for Image Analysis function.
Limitation:




Single images can be transferred by “Analysis” function only.
Gallery and Analysis function are not visible during “Presentation” view.
Gallery cannot show external media files.
Gallery bar functions are limited during Multi-Fluorescence mode session.
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6. Side by Side
Side by Side tool of PROGRES GRYPHAX® software
The “Side by Side tool” of PROGRES GRYPHAX® software enables user to compare images from Gallery
with each other or images with live stream from camera easily inside of GRYPHAX software.
General description:
The Side by Side tool is part of the Gallery tool. The Gallery is located on the left software
side next to the Tree view tool. The Gallery preview window shows thumbnails from the
media files of selected destination folder. The Side by Side tool will be activated by
operating with mouse only and the Gallery has to be opened in advance.
To open or close of the Gallery tool press the bottom arrow
use the keyboard short cut (ctrl / cmd + G).
6.1
on the left software side or
Start Side by Side mode:
To start the Side by Side mode there are three possibilities:
1. Drag and drop an image thumbnail from Gallery over each other
will enable the image comparison of two images. Both images
will be displayed side by side at the main image window of
GRYPHAX software.
2. Select one image from Gallery by double-click on the thumbnail.
Image will be displayed on the main image window. Drag and
drop second image thumbnail from Gallery into the main image
window of GRYPHAX software while first image is displayed. The
image can be added on left or right side to the main window.
3. Drag and drop one image thumbnail into the main image
window of GRYPHAX software while live stream from camera is
running. You can drop on the left or the right side of the live
window. The Side by Side tool will add the Gallery image
according the used side.
Note: Video files are not supported by Side by Side mode.
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Overview:
At the Side by Side mode the image file name of displayed
images will be shown on top of the each image.
If a live image from camera is used for comparing at Side by Side mode the live stream is named with
“Live”
instead of the image file name.
Screen shot of active Side by Side “comparing” mode using two different images from Gallery:
Note: The image size of different image resolution will be displayed scaled to the image main window of
GRYPHAX software. The image with the smaller size is the master, the larger image will be scaled
according the size of smaller image.
6.2
Change images:
To change images at Side by Side mode just drag and drop one image thumbnail into the main image
window of GRYPHAX software while Side by Side mode is active. You can drop the image on the left or
the right side of the main window. The Side by Side tool will exchange the previously displayed image by
the new added image from Gallery according the used side.
6.3
Leave Side by Side mode:
To leave the Side by Side mode just close one of the displayed image by using the cross
click on any image thumbnail at the gallery.
Limitation:


Side by Side mode is not possible with video files
Measurement and Annotation tool are deactivated during Side by Side mode
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or double-
7. Tree view tool
Tree view tool of PROGRES GRYPHAX® software
The “Tree view tool” of PROGRES GRYPHAX® software enables user to save, organize and handle media
destination folder structure easily inside of GRAPHAX software without leaving software.
General description:
The Tree view tool is located on the left software side
next to the Gallery. It can be used with and without
opened Gallery. Tree view delivers two different sizes:
“Narrow” or “Wide” to display the media target tree.
To open, expand or close of the Tree view tool press
the top left arrow
on the left software side or use
the keyboard short cut (ctrl / cmd + D).
Note: After first software start the Tree view tool is
starting and displaying the default destination folder of
operating system “own Pictures” from current user.
Alternatively, the Tree view is automatically displaying
and using the last used destination folder from last
software session or from activated user profile.
The media destination folder of Tree view is always
synchronized with the software preferences settings
under section: storage options.
The currently used destination folder for media storage is displayed with the save icon
and colored
in “blue” characters.
Additionally, all images and media files of selected
destination folder are displayed at the software Gallery by preview thumbnails.
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7.1
Change storage folder:
To change media destination folder please double click by left-hand mouse click on according
destination folder from tree view. After change of destination folder, the Gallery will display all media
files of new selected folder. All new captured images and media files will be save to the selected folder.
7.2
Create storage folder:
To create new folder or subfolders, please make a right-hand mouse
click into the folder tree to activate the Tree view edit option menu:
With mouse click on menu: “Change tree using File Explorer” an operating system specific edit window
will be opened.
Note: At the Tree view folder edit window you can use the typical functions of operating system.
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To add a new folder or subfolder you have to navigate to the folder tree and press button:
“New folder”
A new folder will be created and can be renamed afterwards.
To rename an existing folder please use the operating system function by “right-hand mouse click” and
option: “Rename”.
To delete an existing folder after selection use keyboard key “DEL” or use operating system function by
“right-hand mouse click” and option: “Delete”.
To select the media destination folder for images and media files by double click and define with button:
“Select Folder”
Note: The edit window will close automatically after folder selection by press “Select Folder”.
With close of edit window using button: “Cancel” the software select the default destination folder of
operating system “own Pictures” from current user as media destination folder.
Record to new destination folder
To record new images or media files to new defined destination folder just press “REC” button
start record and the files will be displayed at the GRYPHAX Gallery.
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to
Navigation:
Vertically scrolling via mouse wheel or scroll bars.
view does not fit into available space.
View destination path:
To quickly display the complete destination path of
each folder you can use the tooltip at Tree view tool.
Hold the mouse pointer over according folder of tree
view until complete path will be displayed (hover).
To display the destination path of saved
images from Gallery without Tree view
tool you can use the path information of
“Report” widget. Hold the mouse
pointer over Path of Report widget until
complete path will be displayed (hover).
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Scroll bars appears if current tree
8. Exposure and Histogram
Exposure tool and Histogram of PROGRES GRYPHAX®
software
General description:
The “Exposure tool” of PROGRES GRYPHAX® software enables user to control camera image brightness
manually or automatically to reach optimal exposed images. By using the “Histogram” user can observe
optimal image brightness conditions at a glance.
Overview GRYPHAX software with activated Exposure tool and Histogram:
Open Tool bar:
All GRYPHAX camera control and image processing tools are located at the “Toolbar”.
To activate the “Exposure tool” or “Histogram” open the GRYPHAX “Toolbar” by pressing the arrow
on right-hand software site or use keyboard short cut (ctrl / cmd + T)
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8.1
Open Exposure:
To open Exposure tool click to the Exposure icon
opened and contains two separate mode
at the “Toolbar”, the Exposure widget will be
tabs for:
“Auto” – for automatically brightness control for optimal tracking of brightness by e.g. objective change
“Expert” – for manually brightness control by using Exposure time and Gain value to reach max. exp. time
Auto tab is activated as default and contains the following functions:

Intensity – to adjust image desired target brightness of image
preview from active camera displayed on screen by slider or value

Current exposure time – display current exposure time of live
preview (changing according automatic exposure control)

Ocular view – optional to adapt the color impression from
microscope eyepieces to the image at screen (Color cameras only)

Grey balance pipette – set manually grey balance at live preview

Reset Grey balance – reset grey balance back to factory settings
Expert tab contains the following expert functions:

Time – set exposure time manually by slider or enter value

Gain – set gain value manually by slider or enter value

Ocular view – optional to adapt the color impression from
microscope eyepieces to the image at screen (Color cameras only)

White Point pipette (1) – set manually white point at live preview

Grey balance pipette (2) – set manually grey balance at live preview

Black Point pipette (3) – set manually black point at live preview

8.2
Reset balances (4) – reset back to factory settings
Maximum exposure time
Maximum exposure time for captured images can be reached by using max. exposure time in combination
with max. gain value at “Expert tab”. For e.g. NAOS camera max. live exposure is 1000 ms + max. gain value
of 10 = 10 seconds exp. time for captured images in case that preferences option “Minimize Gain” is activated.
Note: To reset grey balance or black / white point separately, select according option
reset button! Otherwise all balance and points will be reset together.
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before press
8.3
Set Grey Balance:
Activate grey balance
icon from Exposure widget. Mouse pointer immediately
change to pipette pointer and display small magnifier window and pixel values of
according image position at image window.
Click into “grey” region of preview image to set new grey values and the image will automatically
change the grey balance of RGB preview image.
Note: Only available for color cameras.
8.4
Set Black Point:
Activate black point
icon from Exposure widget. Mouse pointer immediately
change to pipette pointer and display small magnifier window and pixel values of
according image position at image window.
Click into “black” (dark image) region of preview image to set manually new black offset and the image
will automatically change the black offset at preview image and the noise level will be reduced instantly.
Important Note: Black point is a very powerful function and will reduce the image bit depth of preview
and captured images! Image can be destroyed or show pixel artefacts. Do not set “Black Point” into
bright image areas to avoid corrupted images!
8.5
Set White Point:
Activate white point
icon from Exposure widget. Mouse pointer immediately
change to pipette pointer and display small magnifier window and pixel values of
according image position at image window.
Click into any region of preview image to set new brightest area at preview image. The software will
automatically set RGB values to max value (255) at related region and the “Expert” Exposure tool change
the exposure time accordingly.
8.4
Open Histogram:
To open Histogram click to the Histogram icon
at the tool bar, the
Histogram widget will be opened and is separated into two different
sections: Histogram section (1) and Pixel value section* (2).
Histogram widget is displaying the following information:



Histogram curve (RGB or Monochrome) according image data
Gamma curve according select Gamma value
Pixel value* of current mouse position (*valid as of version 1.1.12)
Note: “Pixel values” are displayed while mouse pointer is inside of image window only.
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Tool status:


Activated tools are highlighted in blue. Click on tool icon again to deactivate appropriate tool.
Tools which are not access able are “greyed out” by software automatically and cannot be
reached.
Collapse / expand
Reset button
button on each tool to hide separate widgets on active tool section.
on a tool sets all properties to their initial values. Initial values are fixed.
Video tutorial:
Press the link to watch the video tutorial for Exposure tool & Histogram of GRYPHAX software.
Limitations:




Exposure tool is not reachable during Multi-Fluorescence mode.
Exposure tool widget is blocked while video record or time-lapse, Z-stack or Panorama is running.
Exposure mode “Auto” is disabled and cannot be reached during Panorama and Z-Stack mode.
Exposure tool can be activated while image live preview from camera is active only.
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9. Image Enhancement tools – Color & FX
Image Enhancement tools – Color & FX of PROGRES
GRYPHAX® software
General description:
The “Image Enhancement tools” – Color & FX of PROGRES GRYPHAX® software enables user to optimize
images by wide range of techniques to reach perfect images results with true color representation of
specimen.
Color representation of specimen is influenced by a number of factors. In addition of functions white
balance and Ocular view from Exposure tool, enhanced functions available in the tools “Color“ & “FX
(Effects)” can be used to match the image display to the colors and contrasts in the specimen. The FX tool
provides options to enhance image processing using sharpen, noise reduction or high Dynamic function.
Any settings defined here are immediately displayed in the live image preview, and will be applied to the
captured image.
All changes of the Image Enhancement tool settings are saved as preferences “Last Session” and will be
available next time the software is started.
Overview GRYPHAX software with applied High-Dynamic mode from FX tool:
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Open Toolbar:
All GRYPHAX camera control and image processing tools are located at the “Toolbar”.
To activate the Image Enhancement tools “Color” or “FX” open the GRYPHAX “Toolbar” by pressing the
arrow on right-hand software site or use keyboard short cut (ctrl / cmd + T).
9.1
Open Color tool:
To open Color tool click to the Color icon
at the “Toolbar”, the Color widget will be opened and
contains the following image color enhancement tools:

Contrast – to adjust image desired target contrast of image
preview from active camera displayed on screen by slider or value

Saturation – to adjust image desired target saturation of image
preview from active camera displayed on screen by slider or value

Gamma – to adjust image desired target Gamma value of image
preview from active camera displayed on screen by slider or value

Colour Balance – optionally to set manually color balance of image
preview from active camera displayed on screen by slider
Note: To reset separate tool value to initial factory value, double-click on
slider at any position. According slider and value will be reset to default.
Contrast
Use the slider controls to adjust contrast, in the displayed image preview according to your needs.
A low contrast value results in a lower contrast, a high contrast value results in a higher contrast.
A contrast value of 50 is preset as initial factory value, which represent contrast function is off.
Saturation
Use the slider controls to adjust saturation in the displayed image preview according to your needs.
Value of 0 is displaying a completely de-saturated image preview. A high saturation value results in an
over saturated image preview. A saturation value of 50 is preset as initial factory value and color
reproduction will be displayed as neutral.
Gamma
The gamma slider control enables the conversion of camera pixel values into the pixel values displayed in
the image window. A low gamma value results in a brighter, low contrast image, a high gamma value
results in a darker image with higher contrast. The gamma settings do not influence the dynamic range
of your image. The current used gamma curve is visualized at “Histogram” widget and changes will be
immediately applied.
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Note: Gamma adjustment does not reduce the dynamic range of the image. Please note that the preset
gamma value is 0.45 for color cameras, which results in a well-balanced, contrasting color reproduction.
For linear reproduction of the image data, select a gamma value of 1.0 which is used for monochrome
cameras as default.
Colour Balance
Use the slider controls to adjust the color balance in the entire image between an RGB base color and
the respective complementary CMYKcolor. The color balance adjustments will also be kept when a
white balance is carried out using the functions in the Exposure tool.
9.2
Open FX tool:
To open FX (Effects) tool click to the FX icon
at the “Toolbar”, the
Color widget will be opened and contains the following image color
enhancement tools:

Sharpen – to adjust image desired target sharpness of image
preview from active camera displayed on screen by slider or value
A sharpen value of 0 is preset as initial factory value, which
represent sharpen function is off. A high sharpen value results in a
higher sharpness impression.

Dynamic – optionally tool to activate special image dynamic extension by one-image-highdynamic-mode. Which will take advantage of the new generation sensor technology used by
GRYPHAX cameras. It will be applied directly at image preview and to captured images as well.
Note: Dynamic extension mode requires a high demand of CPU performance and can reduce
the live speed performance on certain computers. Please also take note of the system
requirements.

Noise Reduction – to reduce the noise level at image preview from camera by select noise
reduction level by slider. It is recommend to enable noise reduction function especially by high
gain values or long exposure times e.g. Fluorescence applications. The following options can be
selected:

off – no noise reduction enhancement

soft – slightly noise reduction

middle – moderate noise reduction

strong – maximum noise reduction, especially for high gains or long exposure times
Note: FX tools can reduce the live speed performance due to high CPU utilization.
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Tool status:


Activated tools are highlighted in blue. Click on tool icon again to deactivate appropriate tool.
Tools which are not access able are “greyed out” by software automatically and cannot be
reached.
Collapse / expand
Reset button
button on each tool to hide separate widgets on active tool section.
on a tool sets all properties to their initial values. Initial values are fixed.
Video tutorial:
Press the link to watch the video tutorial for Exposure tool & Histogram of GRYPHAX software.
Limitations:


FX tools can reduce the live speed performance due to high CPU utilization.
Contrast and Gamma will be deactivated while FX tool “Dynamic” is active.
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10. Measurement tool
Measurement Tool of PROGRES GRYPHAX® software
The “Measurement tool” of PROGRES GRYPHAX® software enables user to measure basic methods
directly at the live image or at recorded images from Gallery.
General description:
The “Measurement tool” is part of the GRYPHAX software. It contains scale bar and basic measurement
methods as real-time measuring directly at the live preview or at recorded images from Gallery afterwards.
Preparations general:
To use Measurement tool you have to calibrate your microscope objectives in advance! Otherwise the
Measurement tool cannot be activated and used. To create measurement calibration you have to open
GRYPHAX Preferences and navigate to option “Device Configuration”.
To calibrate the microscope you can use the PROGRES GRYPHAX®
calibration slide (order number: 563683).
Please see the video tutorial how to calibrate the microscope objectives:
Click here to watch the Video Tutorial – Device Configuration & Status Bar
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10.1
Start Measurement tool:
To activate the Measurement tool open the GRYPHAX Tool bar by pressing the
arrow on right-hand software site or use keyboard short cut (ctrl / cmd + T)
Click to the Measurement icon
at the tool bar, the Measurement widget
will be displayed and contains the following tools and options:




Line measurement for distances
Freeform measurement for areas and distances* (*as of version 1.1.10)
Circle measurement for radius & diameter
Angle measurement for interior & exterior angles

Measurement unit contains the following units in respect to the used
method: Auto selection as default or nm, µm, mm, cm, m, mil, inch,
ft, deg (°) as well as similar area units
Font size by value 8 – 72px
Outline width by value 1 – 20px
Colour can be selected by color selector, click on color rectangle
to open color selector



Note: Measurement tool is enabled only if record mode “Single shot” is selected and live image is
activated before. Or a recorded image which were captured with calibration information is opened from
Gallery. Images captured without measurement calibration cannot be measured afterwards!
Reset button
10.2
on a tool sets all properties to their initial values. Initial values are fixed.
Pre-define settings:
When no object is current or selected, the tool widgets show default properties. In this state default
properties can be modified. They are stored in settings/profiles.
To predefine the general settings of measurement tool e.g. font size, outline width or colour by change
of the according parameter without selecting any measurement method.
The settings for each drawing object can be individual adjusted after start of drawing or by selecting a
drawn object from image window.
10.3
Start measuring:
To start measuring select record mode “Single shot” and start live by pressing the “Live”-button
open a recorded image from Gallery which is captured with calibration information.
or
By pressing “Rec”
button all previously drawn measurement from live preview will be merge into
the image. And cannot be modified afterwards.
To save drawn measurement at loaded recorded images from Gallery, start Live preview
or load
another image from Gallery. An image copy will be always saved automatically by software with a file
name extension: “_copy_1” and increment the image copy number accordingly.
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Attention: Take care that the correct
“device” and “magnification / objective”
is selected from Status bar before start
with any measuring. Otherwise, the
measurement results are not correct!
10.4
Creation (drawing) of objects:
In general, after clicking one of the measurement method buttons
(distance, freeform, circle or angle), the cursor changes into a cross and user can draw a measurement
object of the type selected.
Additionally, a small floating pane is opened as image window
overlay and shows a detailed view of a selected sector like as the
magnifier tool.
Zoom level of magnifier can be adjusted on the used section of
"zoom level" under Preferences / General / Magnifier
Pressing “Del” or ”Esc” key before finishing an object, aborts the creation and removes unfinished objects.
Each drawn measurement object will be labelled by a fixed prefix name and a counting number
according the object type and quantity.
For further details, please see measurement button descriptions below.
10.5
Draw a distance (line) & freeform measurement:
The line and freeform measurement is a 2-point-measuemrent.
To draw an object start with left mouse-click on first point and release. The start point is marked.
Move the mouse away and a colored semi-transparent line is drawn from the start point to the current
mouse position (refreshed as long as the mouse is inside the image window) additionally the currently
measured value is displayed near the start point.
Go to the end point and make left mouse-click and release.
For freeform the drawn curve will be completed by a straight
line to the start point if end position is different from start
point. The measured value is written near the starting point
(including an auxiliary line) and will be shown the default
unit automatically.
As long as the measurement object is selected the line and
text are semi-transparent. A trash button appears right
beside the measured value and the start- and end points are marked.
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10.6
Draw a radius & diameter (circle) measurement:
The circle measurement is a 3-point-measuemrent which enables to measure circle which are bigger as
the field of view of camera / image.
To draw an object start with left mouse-click on first point and release. The start point is marked.
Move the mouse away and a colored semi-transparent line is drawn from the start point to the current
mouse position (refreshed as long as the mouse is inside the image window)
Go to the second point and make left mouse-click and release.
Move the mouse away and a colored semi-transparent circle is drawn instead of line from the first two
points to the current mouse position. Additionally the currently measured value is displayed near the
start point.
Go to the end point (3rd) and make left mouse-click again and
release. The measured value is written near the starting point
(including an auxiliary line) and will be shown the default unit
automatically.
As long as the measurement object is selected the circle and text
are semi-transparent. A trash button appears right beside the
measured value and the start- and end points are marked.
Note: The measured value can be displayed as diameter or radius (marked by a “D” or “R”). Before the
measurement will be done the user has to make the proper choice.
10.7
Draw an angle measurement:
The angle measurement is a 4-point-measuemrent which enables to measure angle which are bigger as
the field of view of camera / image.
To draw an object start with left mouse-click on first point and release. The start point is marked.
Move the mouse away and a dashed auxiliary line is drawn from the start point to the current mouse
position (refreshed as long as the mouse is inside the image window).
Go to the second point and make left mouse-click and release. The first dashed auxiliary line is drawn.
Go to the 3rd point and make left mouse-click and release. A second dashed auxiliary line is drawn from
this point to the current mouse position (refreshed as long as the mouse is inside of image window).
Go to the end point (4th) and make left mouse-click again and release.
The dashed auxiliary lines becomes continuous line. The measured
value is written near the angular point (including an auxiliary line).
As long as the measurement object is selected the angle and
measurement value are semi-transparent. A trash button appears
right beside the measured value and the start- and end points are
marked.
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10.8
Object selection:
Every object can be selected as the current one at live and captured images before image is recorded /
saved with measurement overlay. The current object is displayed as semi-transparent and decorated with
the trash icon. Most objects (except freeform object) when selected as the current one, also display grey,
square control points which are used to modify the geometry of the object.
An object can be selected as the current one, by clicking it with the pointer tool - 'arrow' button on the
main window's top bar. But when an active tool is other than the pointer tool (e.g. circle drawing tool),
this requires switching the active tool off and switching the pointer tool on. To make it more
convenient, the pointer tool can be temporarily activated by pressing the “ctrl / cmd” key. As long as the
key is pressed, the pointer tool is active. When “ctrl / cmd” is released, the previously active tool
activates again.
The current object can be moved and reshaped (except freeform object) using the pointer tool. When
the annotation or measurement GUI widget is visible on the tool bar, the properties of the current
object (colour, line width, etc.) are set in the widget.
10.9
Object multi-selection:
By marking a rectangular area with the pointer tool at the image window, one can also select more than
one object. Multi-selected objects are also displayed as semi-transparent, but without their trash
buttons. A multi-selection trash will be displayed in center of the selected objects to delete all. Pressing
“del” key remove all selected objects.
Multi-selection can be also created, extended or modified by clicking on objects with the pointer tool
while “shift“ key is pressed. Objects selected can be moved together as well.
10.10 Deleting objects:
The current object can be deleted by clicking its trash icon, or pressing ”del” key. Objects selected can
be deleted together by pressing ”del” key.
10.11 Changing object properties:
Object's properties can be modified using controls on the tool widget appropriate for the object. I.e.
annotation objects (arrow, text) can have their properties modified using annotation tool widget and
measurement objects - measurement tool widget. The properties of the current object are visible in the
widget and can be modified. When more than one object is selected (multi-selection), some common
properties (like color or line width) for all selected objects can be modified.
Note: If a tool widget is closed, double-click on an object makes the object current and opens the
appropriate tool widget at the toolbar.
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Export Measurements:
With “Report tool” of PROGRES GRYPHAX software you can review measurement values, measurement
statistics or export all measurement data from live or recorded images into file.
The “Measurement data” table of Report Tool contains the following statistics:
Average value, minimum value, maximum value and standard deviation (σ) according measurement type.
To export measurements objects open Report tool
and activate the “Measurement data” table
Press button “Export data” to save all measurements data into *.xml file.
.
Note: All functions and possibilities of Report Tool are described separately on Report Tool section at manual.
Limitations:


Measurement and Annotation are not available during time-lapse, video record, Z-stacking,
Panorama or Fluorescence mode.
No measurement available for images captured without measurement calibration data.
Video tutorial:
Press the link to watch the video tutorial for measurement tool of GRYPHAX software.
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11. Scale bar tool
Scale bar tool of PROGRES GRYPHAX® software
The “Scale bar tool” of PROGRES GRYPHAX® software enables user to place scale bar directly at the live
preview or at loaded images from Gallery.
General description:
The “Scale bar tool” is part of the GRYPHAX software. It is available for calibrated cameras and images
from Gallery. It contains a moveable / adjustable scale bar which can directly overlayed at the live preview
or at loaded image from Gallery. Additionally option to add a status bar with scale fitted to the bottom of
recorded images as well.
Overview:
Scale bar placed by mouse drag & drop to loaded image from Gallery.
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11.1
Preparations general:
To use Scale bar tool you have to calibrate your microscope objectives in advance! Otherwise the Scale
bar tool cannot be activated and used. To create measurement calibration you have to open GRYPHAX
Preferences and navigate to option “Device Configuration”.
To calibrate the microscope you can use the PROGRES GRYPHAX®
calibration slide (order number: 563683).
Press the link to watch the video tutorial how to calibrate the microscope
objectives.
After successfully objective calibration the objectives will be added to the magnification list at the status
bar of GRYPHAX software. And the according scale bar will be displayed with value and unit.
11.2
Select unit type and color:
To select preferred unit type you have to open GRYPHAX
Preferences and navigate to section: “Scale bar”. (Available
as of V1.1.10 or newer)
By dropdown menu you can chose between the two options:
 Metric units
 Imperial / US units
The color of scale bar can be predefined and is connected according the color of “Status Bar”. It can be
changed between: “black” or “white”.
11.3
Activate scale bar:
To activate the scale bar from status bar select the correct “Device” and
previously calibrated “Magnification / Objective” according your current
objective from microscope before place of scale bar to the image.
11.4
Place scale into image:
The “Scale” from status bar can be placed by drag & drop via mouse
into the image on any desired position.
Press the link to watch the video tutorial how to drag & drop scale bar
from status bar.
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Alternatively, the feature: “Add Status bar to captured
images” can be activated. A complete status bar will be
added at the bottom of recorded images.
The following information can be add to status bar of
recorded images:
Text | Device | Magnification | Scale bar
Note: Scale bar is enabled only if live image is activated
before. Or a recorded image which were captured with calibration information is opened from Gallery.
Images captured without measurement calibration cannot display scale bar at the status bar. A warning
will be displayed instead.
11.5
Modify scale bar:
After drag and drop, scale can be modified by length and
position at the image. Measurement unit is fixed and
automatically adapt to the length and cannot change by user.
Fond size and style is fixed.
11.6
Delete scale bar:
Scale can be deleted by clicking its trash icon
11.7
, or by pressing ”Del” key after selection by mouse click.
Save scale to live:
The scale on the preview image will be merged into the recorded image by pressing “REC”
And cannot be modified afterwards.
11.8
button.
Save scale to recorded images:
To save a drag & drop scale at loaded recorded images from Gallery, start live preview
another image from Gallery.
or load
An image copy with merged scale will be always saved automatically by software with a file name
extension: “_copy_1” and increment the image copy number accordingly.
Attention: Take care that the correct
“device” and “magnification / objective”
is selected from Status bar before start
with save of scale. Otherwise, the
displayed scale is not correct!
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Limitations:


Drag & drop scale bar is not available during time-lapse, video record, Z-stacking, Panorama or
Fluorescence mode.
No scale available for images captured without measurement calibration data.
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12. Annotations tool
Annotations Tool of PROGRES GRYPHAX® software
The “Annotations tool” of PROGRES GRYPHAX® software enables user to highlight and comment
important areas at images directly at live preview image or at recorded images from Gallery.
General description:
The “Annotations tool” is part of the GRYPHAX software. It contains arrow and text tool to highlight and
comment important areas at images directly at live preview or at recorded images from Gallery
afterwards.
Text tool and arrows applied on loaded z-stack image from Gallery:
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12.1
Start Annotations tool:
To activate the Annotations tool open the GRYPHAX tool bar by pressing the
arrow on right-hand software site or use keyboard short cut (ctrl / cmd + T)
Click to the Annotations icon
at the tool bar, the Annotations widget will
be displayed and contains the following tools and options:


Arrow tool – to add arrows to highlight important locations at images
Text tool – to add comments directly at images




Font Size – by value 8 – 72px
Font Style – by option Normal, Bolt, Italic or Bold + Italic
Outline Width – by value 1 – 20px
Colour can be selected by colour selector, click on colour rectangle
to open colour selector
Note: Annotations tool is enabled only if record mode “Single shot” is selected and live image is activated
before. Or a recorded image is opened from Gallery. No support for Time-lapse preview or video files.
Reset button
12.2
on a tool sets all properties to their initial values. Initial values are fixed.
Pre-define settings:
When no object is current or selected, the tool widgets show default properties. In this state default
properties can be modified. They are stored in settings/profiles.
To predefine the general settings of Annotations tool e.g. font size, outline width or colour by change of
the according parameter without selecting any Annotations method.
The settings for each drawing object can be individual adjusted after start of drawing or by selecting a
drawn object from image window.
12.3
Start & save drawings:
To start drawing select record mode “Single shot” and start live by pressing the “Live”-button
open a recorded image from Gallery.
or
By pressing “Rec”
button all previously drawn annotations from live preview will be merge into the
image and cannot be modified afterwards.
To save drawn annotations at loaded recorded images from Gallery, start live preview
or load
another image from Gallery. An image copy will be always saved automatically by software with a file
name extension: “_copy_1” and increment the image copy number accordingly.
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12.4
Creation (drawing) of objects:
In general, after clicking one of the annotations method buttons
changes and user can draw an annotation object of the type selected.
(Arrow or Text), the cursor
Additionally, a small floating pane is opened as image window overlay
and shows a detailed view of a selected sector like as the magnifier tool.
Zoom level of magnifier can be adjusted on the used section of "zoom
level" under Preferences / General / Magnifier (valid as of version 1.1.8)
Pressing “Del” or ”Esc” key before finishing an object, aborts the
creation and removes unfinished objects.
For further details, please see annotations button descriptions below.
12.4.1 Draw an arrow:
The arrow tool
is a 2-point drawing object.
To draw an object start with left mouse-click on first point and release. The start point is marked.
Move the mouse away and a colored semi-transparent arrow is drawn
from the start point to the current mouse position (refreshed as long
as the mouse is inside the image window).
Go to the end point and make left mouse-click and release.
As long as the annotation object is selected the arrow is semitransparent. A trash button appears right beside the end point. The
start- and end points are marked.
12.4.2 Draw a text field:
To draw a text
object click with left mouse-click on point of interest and release. The text field is
applied and ready to fill in with text. The size of field will be adapt automatically according the insert text.
As long as the text object is selected the text
are semi-transparent and a rectangle border
is visible. A trash button appears right beside
the start point.
By click again into the image, a new text object will be added to the image.
Note: Text objects can contain letters, numbers and special characters as well.
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12.5
Object selection:
Every object can be selected as the current one at live and captured images before image is recorded /
saved with annotations overlay. The current object is displayed as semi-transparent and decorated with
the trash icon. Most objects (except text object) when selected as the current one, also display grey,
square control points which are used to modify the geometry of the object.
An object can be selected as the current one, by clicking it with the pointer tool - 'arrow' button on the
main window's top bar. But when an active tool is other than the pointer tool (e.g. text tool), this
requires switching the active tool off and switching the pointer tool on. To make it more convenient, the
pointer tool can be temporarily activated by pressing the “ctrl / cmd” key. As long as the key is pressed,
the pointer tool is active. When “ctrl / cmd” is released, the previously active tool activates again.
The current object can be moved and reshaped (text object only moved) using the pointer tool. When
the annotation GUI widget is visible on the tool bar, the properties of the current object (colour, line
width, etc.) are set in the widget. Note: For Arrow object only start point can be modified.
By double-click at text object the content can be modified as long as the object isn’t merged to the image.
12.5.1 Object multi-selection:
By marking a rectangular area with the pointer tool at the image window, one can also select more than
one object. Multi-selected objects are also displayed as semi-transparent, but without their trash
buttons. A multi-selection trash will be displayed in center of the selected objects to delete all. Pressing
“del” key remove all selected objects.
Multi-selection can be also created, extended or modified by clicking on objects with the pointer tool
while “shift“ key is pressed. Objects selected can be moved together as well.
12.6
Deleting objects:
The current object can be deleted by clicking its trash icon, or pressing ”del” key. Objects selected can
be deleted together by pressing ”del” key.
12.7
Changing objects properties:
Object's properties can be modified using controls on the tool widget appropriate for the object. I.e.
annotation objects (arrow, text) can have their properties modified using annotation tool widget. The
properties of the current object are visible in the widget and can be modified. When more than one
object is selected (multi-selection), some common properties (like color or line width) for all selected
objects can be modified.
Note: If a tool widget is closed, double-click on an object makes the object current and opens the
appropriate tool widget at the toolbar.
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Limitations:


Annotations are not available during record of time-lapse, video, Z-stacking, Panorama or
Fluorescence mode.
Annotation tool is enabled only if record mode “Single shot” is selected and live image is
activated before. Or a recorded image is opened from Gallery. No support for Time-lapse
preview or video files.
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13. Report tool
Report tool of PROGRES GRYPHAX® software
The “Report tool” of PROGRES GRYPHAX® software enables user to review and export measurement
data, software settings and image meta-data directly at GRYPHAX software.
General description:
The “Report tool” is part of the GRYPHAX software. It enables user to review and export measurement
data from live and recorded images, current software settings and media files meta-data directly at
GRYPHAX software.
Preparation:
To use all feature of Report tool you have to calibrate your microscope objectives in advance! Otherwise
the Report tool cannot be display measurement data information. To create measurement calibration
you have to open GRYPHAX Preferences and navigate to option “Device Configuration”.
To calibrate the microscope you can use the PROGRES
GRYPHAX® calibration slide (order number: 563683).
Overview:
Report tool contains the data tables and views for:



Measurement data table & statistics
Image meta-data view
Software settings view
Show image information from selected media file
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13.1
Start Report tool:
To activate the Report tool open the GRYPHAX Toolbar by pressing the arrow
site or use keyboard short cut (ctrl / cmd + T)
on right-hand software
Click to the Report icon
at the toolbar, the report widget will be opened and contains the
following tools and information of live preview or from selected media file from Gallery:

Measurement data table – shows measurement data and statistics for each measurement
methods and offers option export as file

Image meta-data view – shows image meta-data information and offers option export as file

Software settings view – shows software settings and offers option export as file






Name: – display media file name of selected media file
Path: – display media file destination folder of selected media file
Date: – display creation date of selected media file
Size: – display media file resolution of selected media file or live preview
Exp. Time: – display used exposure time of selected media file
Gain: – display used Gain value of selected media file
Note: Image size is shown at image information only at report widget, during display of live preview
from camera.
Show destination path:
To display the destination path of saved media
files from Gallery without Tree view tool you
can use the path information of Report widget.
Hold the mouse pointer over path of Report
widget until complete path will be displayed
(hover).
Show name:
To display the complete name of saved media files from Gallery
without Tree view tool you can use the name information of Report
widget. Hold the mouse pointer over name of Report widget until
complete name will be displayed (hover).
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13.2
Software settings view:
All software settings of GRYPHAX software are
stored permanently during software operation.
The current software settings can be reviewed
und exported by report tool.
To view current software settings of GRYPHAX
software, open Report tool
and activate
the Software settings view:
To export software settings, press button:
“Export data” to save all current software
settings into *.txt file.
13.3
Measurement data table:
With Measurement data table of Report tool user can review measurement values, measurement
statistics or export all measurement data from live or recorded images into file.
The Measurement data table contains the following
statistics related to selected measurement type:




Average value
Minimum value
Maximum value
Standard deviation (σ)
To view measurements objects, open Report tool
and activate the Measurement data table:
To export measurements data, press button: “Export
data” to save all measurements data information into
*.xml file.
Note: To open exported *.xml file a 3rd party software
like MS Excel or comparable is needed.
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13.3.1 Select or delete measurements at data table:
As long as the displayed image is not
saved by “REC” button. It is possible to
select, change color or delete
measurements from data table directly.
To select make a left-hand mouse click
at the according measurement. The
measurement will be marked in blue
and displayed semi-transparent at the
image window. Also selected
measurements from image window
will be marked at data table as well.
To change color or delete make a
right-hand mouse click. A submenu will
be opened.
13.4
Image meta-data view:
All files recorded by GRYPHAX software are saved with
meta-data information depending on file format. The
meta-data are saved as XMP meta-data and can be
reviewed and exported by report tool.
To view meta-data of media files, open Report tool
and activate the Image meta-data view:
To export image meta-data, press button: “Export data”
to save all meta-data information into *.xml file.
Note: To open exported *.xml file a 3rd party software
like MS Excel or comparable is needed.
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Example file:
Example for exported measurement data file opened by MS Excel.
Limitations:



Report tool are designed to work with media files created by PROGRES GRYPHAX® software only.
During live preview from camera, image size information is shown at Report widget only.
No measurement meta-data available for images captured without measurement calibration data.
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14. MonoChrome mode
MonoChrome mode of PROGRES GRYPHAX® software
The “MonoChrome mode” of PROGRES GRYPHAX® software enables user to converts a color image preview
to a grayscale or monochrome (black & white) image.
General description:
The “MonoChrome mode” is part of the GRYPHAX software. It is available for all color cameras to
become details more visible. This mode provides manipulation to converts a color image to a grayscale or
monochrome (black & white) image. It is applied for live preview from camera and to recorded images.
MonoChrome mode applied at live preview from NAOS color camera:
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14.1
Start MonoChrome mode:
To activate the MonoChrome mode open the GRYPHAX tool bar by
pressing the arrow on right-hand software site or use keyboard short cut
(ctrl / cmd + T)
Click to the MonoChrome mode icon
at the tool bar, the color image
preview will be converted into grayscale or monochrome (black & white)
image.
Additionally, the Histogram changes to grayscale mode and display image
information as monochrome image.
Note: MonoChrome mode is enabled only if any record mode like “Single
shot” is selected and live image is activated before. It cannot applied to
already recorded images from Gallery afterwards.
Start & save converted images:
To start - select record mode e.g. “Single shot” and start live by pressing the “Live”-button
Press MonoChrome mode icon
.
at the tool bar to activate image converting.
By pressing “Rec”
button concerted color to greyscale image will be saved to pre-selected
destination folder and will be visible at software Gallery. Recorded converted images cannot be modified
back to color images afterwards.
Note: By using MonoChrome mode the images will be saved as single channel greyscale images. The file
size is reduced threefold depending to selected file format.
14.2
Deactivate MonoChrome mode:
To deactivate MonoChrome mode by pressing “MonoChrome” button
again, colored image
preview will be displayed again. Histogram change back to RGB mode to display each color channel
separately.
Limitations:



MonoChrome mode is available for color cameras only.
MonoChrome mode is not available during Fluorescence mode session
MonoChrome mode can be enabled only if record mode “Single shot”, “Time-lapse” or “Video”
is selected and live image preview is activated before.
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15. Fluorescence tool
Multi-Fluorescence tool of PROGRES GRYPHAX®
software
The “Multi-Fluorescence tool” of PROGRES GRYPHAX® software enables user to create single or multi-color
fluorescence images. Also you can merge RGB and colored fluorescence images easily.
Note: The Multi-Fluorescence tool is available for both monochrome and color cameras. Color cameras
will be automatically set to the monochrome mode when this function is activated.
15.1
Preparations general:
Important note: All camera image parameter (e. g. capture resolution and storage format) should be set
in advance before you start with Multi-Fluorescence image tool.
We are recommend to use “16 bit” image format to reach best image results with Fluorescence tool.
In case of scanning camera use, please set the capture resolution to a non-scanning mode under
“Preferences/Device Configuration”. Otherwise the image capturing time can be up to 36 minutes
depending on used Exposure Time and Gain value!
To reach to best results with Fluorescence tool you have to activate the option “Minimize Gain during
Record”.
To enhance the image results of long exposed image, we strongly recommend to create a so called:
“black shading” correction to reduce the noise level before starting fluorescence image capture.
The Multi-Fluorescence tool is designed to save up to 10 different filter
parameter settings sets.




Filter set 1 to 7 for different Fluorescence filter
PH settings for Phase Contrast
DIC settings for Differential Interference Contrast
And RGB settings for colored images from color cameras
Note: All user interaction and changes of filter set parameter will be saved after change accordingly.
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15.2
Overview:
Multi-Fluorescence tool contains two separate
windows:


Filter list with active filter sets
Settings widget which contains all
camera parameter
Fluorescence filter set (1) of 7 different available
set-ups can be changed by filter menu settings.
Dropdown filter menu
to change filter set
number, order and position at Fluorescence tool
filter list.
Checkbox
to activate or deactivate filter set
to combine at “merge” image.
Intensity slider
to adjust
the filter intensity after image record. To correct
brightness on merge image.
Color selector
- by click on the color icon the
“Wavelengths Selector” will be opened and you
can enter the exact emission wavelengths in nm
for the appropriate filter set.
Add new filter - click on empty filter set to add
new filter to Fluorescence tool filter list.
Arrows
Fluorescence filter list.
to scroll manually at
Preview thumbnail of “merged”
depending on selected filter set.
image,
Trash
to delete selected filter set from
Fluorescence filter list. All previously settings will
be saved and reused after add filter set number
again.
Filter specific camera settings are connected to each separate filter set.
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15.3
Status of filter set:
To support different interactions, the filter set thumbnails indicates the following status of filter set.
Red frame rectangle indicates ready for record. All image parameter of
Fluorescence tool are active to adjust all parameter. The
corresponding live image is activated and displayed on the image
main window of GRYPHAX software.
Press “REC” button
to save filter set image into the temporary
image list of Fluorescence tool. The Filter set preview thumbnail will
be updated accordingly.
Blue frame rectangle indicates displaying of already recorded filter set
image. The recorded image is activated and displayed on the image
main window of GRYPHAX software. The image parameter of
Fluorescence tool are deactivated.
Press “REC” button
to save filter set image into the Gallery.
After successful save of filter set image the preview thumbnail will be
marked with a save icon
.
Grey frame rectangle indicates that no interaction can be performed.
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15.4
Start & initial set-up:
After successfully preparation you can open “Multi-Fluorescence tool” by click on Fluorescence icon
at Tool bar.
Note: To start a new Fluorescence session you have to toggle the icon
, all settings of filter set will
be saved and the previously recorded images are removed and cannot be re-loaded.
At the first use of Multi-Fluorescence tool you have to set-up your specific filter set-up of Microscope.
No initial filter set is displayed.
1) Add new filter set with click on empty filter thumbnail a new
filter set will be added to the filter list Fluorescence tool. Add
the number of necessary filter sets according to your number
of fluorescence filter.
2) Change Emission wavelength by click on the Color selector
the “Wavelengths Selector” will be opened and you can enter the exact
emission wavelengths in “nm” for the filter set / fluorochromes.
With “Accept” of the selected emission wavelengths, the wavelengths is
saved to the filter set and the recorded monochrome image will be
colored with this color after press “REC”
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button.
3) Adjust camera specific parameter separately for each created filter
set.
4) Press “REC” button
to start record filter set image into the filter
set. The already recorded filter set images will be displayed at the
merge preview thumbnail and updated after change or add of each
filter set image.
5) Fluorescence tool will jump to the next “empty” filter set
automatically. In order from top to button of the filter list.
6) Repeat the points 2. to 4. to fill-up all filter set.
7) After record of each filter set image the software jump to the merge
preview thumbnail and will display the merge image at the image
window of GRYPHAX software.
8) Press “REC” button
to save merge image to Gallery. The save
icon
will display that the currently displayed “merge” image is
saved to the Gallery.
9) With the intensity slider
of each filter set you
can adjust the filter image brightness afterwards according to your
needs.
10) To save the adjusted “merge” image, press “REC” button
to save new “merge” image into the GRYPHAX Gallery.
15.5
again
Re-record images:
In case that previously captures images are not acceptable, user can
“re-record” filter set images with single click into the filter set
thumbnail at any time. Red frame is displayed to the filters set. The live
image is started with previously used parameters on image windows
again. All image parameter can be adjusted now and will be applied to
the image preview.
Press “REC” button
to start record a new filter set image into the filter set.
The preview thumbnail and the “merge” image will be updated according to the new filter set image.
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15.6
Save to Gallery:
To Save “Merged” image - click onto the “Merged” image thumbnail. The blue frame rectangle will be
visible at “Merged” thumbnail at fluorescence filter list.
Press the “REC”
button to save the composed merge image to the GRYPHAX Gallery, at any time
if you are satisfied with the result image. After successful save of “merge” image the thumbnail will be
marked with an save icon
.
To Save “Filter set” image – double click to the filter set thumbnail at any time during Fluorescence tool
session is active. The blue frame rectangle will be visible at “filter set” thumbnail.
Press the “REC”
button to save the filter set image to the GRYPHAX Gallery. After successful save
of “filter set” image the thumbnail will be marked with an save icon
.
In case of large image size the remaining time to save the images will be
displayed. The image record can be aborted by press “Cancel” button.
Additional hints:
Show emission wavelengths of filter set via mouse over. Hold the
mouse pointer over according color box. The tool tip will display
the currently selected emission wave lengths.
Limitations:



Measurement and Annotation are not allowed during Fluorescence tool.
Preferences and Gallery are not reachable during Fluorescence tool.
The number of images and filter set for Fluorescence tool is limited by approx.: 2 GB of memory.
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16. Panorama tool
Panorama tool of PROGRES GRYPHAX® software
The “Panorama tool” of PROGRES GRYPHAX® software enables user to create large images of specimen
without motorized x/y stages at live!
16.1
Preparations:
To reach to best results with Panorama tool you have to adjust the alignment of camera related to the
stage before you start using Panorama tool.
To adjust the alignment you can use the “Grid” function
(keyboard short cut “g”) and the PROGRES GRYPHAX®
calibration slide (order number: 563683).
Grid overlay on live image to adjust alignment:
Note: To improve the panorama image creating the camera should deliver the highest live frame rate.
Reduction of live resolution and exposure time enables highest live frame rate. Furthermore, deactivate
of FX tools “Sharpen”, “Dynamic” and “Noise reduction” increases live frame rate as well.
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16.2
Start:
After successfully preparation you can open “Panorama tool” by click on Panorama icon:
To define the Panorama start position for image combining, please move the live rectangle via drag &
drop to the start position.
Press “REC” button
to start panorama image record.
The Panorama tool change to ”painting mode” (this is the basic mode). The live rectangle shows a red
frame and the tool is observing the live image for movement of the specimen. Additionally, the “REC”
button change to “STOP”
to save the composed image at any time if you are satisfied with the
panorama image. After save of panorama image the software returns back to Panorama tool to start a
new image creation.
To start image combining please move the microscope stage gently to
compose panorama image by a meander loop like as follows and keep an
overlap of 1/3 of the live rectangle!
During stage movement the Panorama tool detects and tracks the movements and accordingly moves
the live rectangle on the background. The image is painted (stamped) on the background creating
panorama. The stamp happens when the rectangle reaches some distance since the last stamp. Note,
that the image must be stable during stamping to avoid blurring. Therefore user should move the stage
gently, pausing occasionally to allow stable images. If the tracking is lost for any reason, the tool goes to
"recovery mode".
“Panorama tool” at painting mode:
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16.3
Lost matching:
In case of lost matching point the tool change to “recovery mode” the live preview rectangle displays
semi-transparent live image from the camera. The camera button continue shows the "REC" label.
Furthermore, the software tries to find a match between the current live image from the camera and the
surrounding background. When the match is found, the live rectangle jumps to the matched position
automatically and the state changes to “painting mode” again. Now you can continue with panorama
image combining.
Hint: During “recovery mode” you should move the microscope stage to some previous position, so the
image will show an area which has recently been painted into the background. Then the match can be
easily found and painting will continue from the position where tracking had been lost.
If the "REC"
button is pressed during recovery mode, the tool goes back to “painting mode” from
the current position immediately. And you can continue with image combining.
16.4
Manual mode:
At any time, user can drag the live rectangle on the background area using the mouse. After dragging,
the “manual mode” is activated. The live rectangle displays semi-transparent live image from the camera
and the camera button continue shows the "REC" label.
After dropping, the image combining can be continued by pressing the "REC"
button. When user
hover the live rectangle on the background area where the match between the background and the
image can be found, the matched area is marked by a white border rectangle. If during that, user drops
the rectangle, it snaps to the matched area and the painting mode activates automatically!
“Manual mode” can be used to start the panorama composition from an arbitrary position on the
background, or to continue painting from well-defined position in a case, when tracking has completely
lost.
“Panorama tool” at manual mode:
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Limitations:
a) The image size for Panorama images is limited to approx.: 120 MP (mega pixels)
The software will display a warning message as follows:
Please stop painting image and save the panorama image by using “STOP”
button.
b) The movement detection and tracking algorithm is based on well-defined features (details) in the
live image. It cannot work well when those features cannot be found i.e:




When the image shows empty, even areas. Very bright or very dark.
When the image is blurry or out of focus.
When the image shows a repetitive pattern (e.g. grid) so identical features appear
everywhere.
When the framerate is low due to a high camera resolution or high exposure time.
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17. Z-Stacking tool
Z-Stacking tool of PROGRES GRYPHAX® software
The “Z-Stacking tool” of PROGRES GRYPHAX® software enables user to create EDF (extended depth of
focus) images of specimen without motorized z-stages at live! The sharp areas of the specimen are
automatically recognized and combined to one consistently sharp, so called, EDF image.
17.1
Preparations:
All camera image parameter (e. g. colour, white balance) must be set in advance before you start with
Z-Stacking image combining tool.
To reach to best results with Z-Stacking tool you have to adjust the focus level of specimen to the top
focus point and change to “Expert” exposure control before you start using Z-Stacking tool.
To find the best focus start point you can use the focus indicator bar of “Magnifier tool”
(keyboard short cut: space bar)
Magnifier tool with “blue” focus
indicator bar:
Note: The zoom factor of
“Magnifier tool” can be adjusted at
the software preferences.
Note: To improve the Z-Stacking image creating the camera should deliver highest live frame rate.
Reduction of live resolution and exposure time enables highest live frame rate. Furthermore, deactivate
of FX tools: “Sharpen”, “Dynamic” and “Noise reduction” increases live frame rate as well.
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17.2
Start:
After successfully preparation you can open Z-Stacking tool by click on Z-Stacking icon:
Z-Stacking tool delivers two different composition modes:
a) “Fast” – very fast image composition without correction for
parallax shift
b) “Precise” – high quality image composition with parallax shift
correction of e.g. stereo microscopes
Please define the Z-Stacking composition mode for image combining before you start image combining!
Press “REC” button
to start Z-Stacking image combining.
The “Z-Stacking” tool is displaying the split screen. On the left the composed result preview image and
on the right site the live image getting from camera.
EDF preview image
The image counter
Live image from camera
is displaying the current number of used different focus images.
Additionally, the “REC” button change to “STOP”
to save the composed EDF image.
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To start image combining please move the microscope stage at “Z-axis”
gently to compose “Z-Stacking” image starting from top focus to bottom
like as follows in one not changing direction.
During stage movement the Z-Stacking tool detects and tracks the sharp image areas automatically and
accordingly save the different focus images to the internal memory. The result preview image is painted
and shows where the Z-Stacking tool has found different sharp area and combined them to one image.
17.3
Save:
Press the “STOP”
button to save the composed EDF image to the GRYPHAX Gallery, at any time if
you are satisfied with the result image. After save of EDF image the software returns back to Z-Stacking
tool to start a new image creation.
With composition mode “Fast” the EDF image will be saved immediately after press “STOP” to the
Gallery.
With composition mode “Precise” the EDF image will be generated from the current
number of different found focus images. This will be more time-consuming.
The remaining time will be displayed.
The image calculation can be aborted by press “Cancel” button.
Limitations:



Measurement and Annotation are not allowed during “Z-stacking tool”.
Save of separate focus layer images are not available.
The image number for Z-Stacking images is limited by approx.: 2 GB of memory
The software will display a warning message as follows:
Please stop image combining and save the Z-Stacking image by using “STOP”
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button.
18. Grid mode
Grid mode of PROGRES GRYPHAX® software
The “Grid mode” of PROGRES GRYPHAX® software enables user to overlay a Grid or crosshairs over the
live image stream or recorded items from Gallery.
General description:
The Grid mode is part of the GRYPHAX software. It enables user to overlay a grid or crosshairs over the
live image stream or recorded items from Gallery. The displayed grid or crosshairs will be not saved to
the recorded images or video files.
Use the grid to adjust the alignment of specimen to the image stream of camera. See also example
below. Additionally, the crosshairs will display the center of the image.
Grid mode activated:
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18.1
Start Grid mode:
To start the Grid mode use the keyboard key (G) or start
by using the title bar menu at GRYPHAX software under
View / Grid.
Note: This submenu is enabled if any meaningful image/video is shown in the image window.
Change line distance:
To change the line distance of Grid
use the mouse wheel and scroll up
to enlarge the factor or scroll
down to lower the factor.
Additionally, a crosshairs would be
displayed only if the line distance
factor reach the maximum image
width or height. See adjacent
screen shot.
Note: The color of cross is fixed to
black.
Change color:
To change the color of Grid, open Preferences from title bar and change the color of Grid section under
Preferences / Elements of Style / Grid
Leave Grid mode:
To leave the Grid mode use the keyboard key (G) or by using the title bar menu at GRYPHAX software
under View / Grid.
Note: Last used line distance factor and grid colour will be stored at software settings and are reused
after start of Grid mode again.
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Example for Grid mode:
To reach to best results with e.g. Panorama tool you have
to adjust the alignment of camera related to the stage.
To adjust the correct alignment you can use the Grid
function and the PROGRES GRYPHAX® calibration slide
(order number: 563683).
Grid overlay on live image to adjust alignment:
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19. Presentation mode
Presentation mode of PROGRES GRYPHAX® software
The “Presentation mode” of PROGRES GRYPHAX® software enables user to display the live image
stream or recorded items from Gallery on whole screen to see all details of images.
General description:
The Presentation mode is part of the GRYPHAX software. It enables user to change from standard
software view to full screen mode to display the live image stream or recorded items on whole screen.
All software tools will be still active at the background but not visible.
Presentation mode activated:
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19.1
Start Presentation mode:
To start the Presentation mode use the keyboard key
(F11) or start by using the Title bar menu at GRYPHAX
software ...View/ Presentation Mode.
Note: This submenu is enabled if any meaningful image/video is shown in the image window.
Change zoom factor:
To change the zoom factor at Presentation mode
between “1:1” view and scaled “Fit To Screen” view use
the keyboard key (Z). The Default view at GRYPHAX
software is “Fit To Screen” view. At “1:1” view the image
will be display one pixel of the camera/ image as one pixel
on the screen.
Additionally, a small overview window will be displayed over the image
at “1:1” view. The currently displayed part of image is marked as
rectangle. The position (ROI) can be moved by mouse operation. Also
size and position of overview window can be changed by mouse
operation.
Activate magnifier:
To activate the Magnifier tool
during Presentation
mode use the keyboard key (Space bar).
Hold the space bar and a small floating pane is opened as
image window overlay and shows a view of a selected
sector as long as the key is pressed.
Zoom level is depending on the used section of "zoom
level" under Preferences / General / Magnifier
Record images:
To record images during Presentation mode you can use the keyboard key (Enter) or (F2).
All images will be recorded to previously selected destination folder and can be previewed at software
Gallery.
Leave Presentation mode:
To leave the Presentation mode use the keyboard key (F11) or (ESC). Software will change back to the
standard software view with all last opened tools as before.
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20. Video mode
Video mode of PROGRES GRYPHAX® software
The “Video mode” of PROGRES GRYPHAX® software enables user to record video files in high image
quality and video speed.
General description:
The Video mode is part of the GRYPHAX software. It enables user to record video files in high image
quality and video speed. The video resolution is fixed to the used camera type and aspect ratio.
Additionally, user can replay / review recorded video files from gallery.
Video replay mode activated:
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Preparations general:
No additional preparation are necessary, all software settings will be used from current settings.
20.1
Start Video mode:
To activate the Video mode use the video mode icon
on
the tool bar. All camera settings and tools are enabled to
adjust the current camera image by enabling additional tools
as well.
A video widget will be opened and display the recording time and
video record resolution.
Note: Change to record mode is enabled only if live image is
activated before and live stream is shown in the image window.
To start the video record by pressing the “REC”-Button
signal the user, that the recording mode is active.
, which instantly changes to “STOP” to
Note: During the recording mode only a few additional tools can be activated.
Save Video file:
Press the “STOP”
button to save the recorded video file to the GRYPHAX Gallery, at any time if
you are satisfied with the results. After save of video file the software returns back to Video record mode
to start a new video record in case that the function “Show image after capture” is deactivated under
Preferences / Storage Options. Keyboard short cut (ctrl / cmd + O).
If the video file saving will be time consumption, the remaining time to save the file will
be displayed.
The video saving can be aborted by press “Cancel” button.
With the storage option “Auto save” under Preferences / Storage Options all recorded video files will be
saved with an automatic given naming structure to the selected destination folder. The file name
contains the: prefix, date, time and the file extension separated by an underline “_”. This structure can´t
be changed by user. The prefix for video files is “Video” e.g.: Video_date_time.file extension.
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Leave Video mode:
To leave the Video mode use the alternative record modes
“Single shot” or “Time-lapse” icons from the tool bar.
Note: Last set software settings will be still used and saved at
software settings.
20.2
Replay Video files:
To replay / playback video files from gallery, double-click on the according video file thumbnail. The
video file will be displayed on the image main window together with an overlayed function bar.
The video replay function bar contains the following options:
a) Video controls for Back / Play / Pause / Forward.
b) Progress bar to display position of video file or to seek the whole video file by mouse operation.
c) Display the time-stamp and total video time.
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20.3
Change storage format:
To change the storage format for video files, please enter the software Preferences at title bar under
“Storage Options” or use keyboard short cut (ctrl / cmd + O).
Note: The available video storage format of the cameras depends on the operating system.
Limitations:





Measurement and Annotation are not available during video record.
Video resolution is fixed to the used camera type and aspect ratio.
The video file size is limited by approx.: 2 GB of memory.
The max video frame rate is limited up to 25 fps depending on used hardware environment.
The max exposure time is limited to 33 ms. Gain can be used instead.
Video tutorial:
Press the link to watch the video tutorial for video mode of GRYPHAX software.
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21. Time-lapse mode
Time-lapse mode of PROGRES GRYPHAX® software
The “Time-lapse mode” of PROGRES GRYPHAX® software enables user to capture time controlled
image sequences by a specified time duration or within a given time interval.
General description:
Some applications require the time controlled capture of image sequences.
The necessary settings for these applications can be set with the functions at “Time-lapse mode”.
Time interval and number of images can be selected. All time settings in this record mode are set in
seconds. When the software is closed, the current settings are always saved and will be automatically
loaded when the software is restarted.
Overview:
Time-lapse replay mode activated:
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21.1
Start Time-lapse mode:
To activate the Time-lapse mode open the GRYPHAX toolbar by pressing the arrow
software site or use keyboard short cut (ctrl / cmd + T)
on right-hand
Click to the Time-lapse icon
at the toolbar, the Time-lapse widget
will be displayed and contains the following information and options:

"Image count" – total image number you want to record

"Duration time" – specifies the intended time for complete record

"Interval time" – specified the intended time between image record

Optionally, a start “Delay” time can be defined
The first three options depending on each other!
The selected values of option which has the active point "dot" is fixed and
can’t be changed unless the “dot” will be moved to other option.
As example: User can set the "Image count" to a value of total images
e.g. 100 and change afterwards the "dot" to:
"Image count".
So the value of 100 images is fixed and all other changes of values depending on the fixed "Image
count" value! If another value is changed, the respective value will be adjusted automatically to the
settings.
At the bottom of Time-lapse widget the information about running image sequence will be displayed for
leftover: time and image number for sequence. And the currently used image resolution.
Image resolution for sequence image record is connected to the capture resolution from preferences.
To change the resolution open preferences under Device Configuration.
Additionally, we recommend to activate option: “fast capture”* for time-lapse image sequence record.
*(valid as of version 1.1.10 and newer)
Reset button
on a tool sets all properties to their initial values. Initial values are fixed.
Important note: “Time-lapse” mode is enabled only if record mode “Multi-Fluorescence”, “Z-Stacking” or
“Panorama” are deactivated and live image is activated before.
21.2
Change storage format:
To change the storage file format for time-lapse image files, please enter the software Preferences at
title bar under “Storage Options” or use keyboard short cut (ctrl / cmd + O).
Note: The saving time for each time-lapse image depends on the used storage format of the cameras.
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21.3
Start Recording:
To start the image sequence record by pressing the “REC”-Button
“STOP” to signal the user, that the recording mode is active.
, which instantly changes to
During image sequence record, the live preview will be deactivated and each single image will be
displayed on image window.
Additionally, the leftover amount of recording time and image number will
be displayed on the time-lapse widget.
After save of image sequence files a new time-lapse sequence thumbnail will
be created at Gallery. On hard drive a separate folder with date- and timestamp will be created automatically which contains all images from time-lapse
sequence record.
The software returns back to Time-lapse record mode and showing live preview to start a new sequence
record in case that the function “Show image after capture” is deactivated under Preferences / Storage
Options. Keyboard short cut (ctrl / cmd + O).
Time-lapse is using storage option “Auto save” under Preferences / Storage Options. All recorded image
files will be saved with an automatic given naming structure to the selected destination folder. The file
name contains the: image number (beginning with “0”) date, time and the file extension separated by
an underline “_”. This structure can´t be changed by user.
Note: During time-lapse recording mode only a few additional tools can be activated. Option “manual
save” will be ignored for image sequence record if selected.
Abort time-lapse record:
Press the “STOP”
button to abort image sequence record to the GRYPHAX Gallery.
Already recorded time-lapse images will be still saved anyhow.
Leave time-lapse mode:
To leave the Time-lapse mode use the alternative record modes
“Single shot” or “Video” icons from the tool bar.
Note: Last set software settings will be still used and saved at
software settings.
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21.4
Replay Time-lapse sequences:
To replay / playback image sequence files from gallery, double-click on the according time-lapse
thumbnail. The image sequence files will be displayed on the image main window together with an
overlaid function bar.
The time-lapse replay function bar contains the following options:
a) Image controls for Back / Play / Pause / Forward
b) Progress bar to display position of image files or to seek the whole image files by mouse
operation.
c) Display of current image number of total image sequence.
Video tutorial:
Press the link to watch the video tutorial for Graphical User Interface of GRYPHAX software.
Limitations:



Measurement and Annotation are not available during Time-lapse image record.
The max sequence frame rate is limited due to used resolution and capture mode and on used
hardware environment (hard drive).
No afterwards measurement available for images captured without measurement calibration data.
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22. System requirements
System requirements - PROGRES GRYPHAX®
software & USB 3.0 microscope cameras
Beside of the latest software version on the PROGRES GRYPHAX® memory card (included in delivery of
the PROGRES GRYPHAX® cameras), we offer a free download after registration.
PROGRES GRYPHAX® microscope camera software
DOWNLOAD
Note 1: For software and driver installation is temporary administrator permission necessary.
Please contact customers IT department in advance!
Note 2: For software operation it is required permission of „full control” for operating system folders below:
Windows OS
Mac OS
Linux OS
"C:\ProgramData\Jenoptik\..."
"MacHD\Library\Application Support\Jenoptik\..."
"/var/lib/Jenoptik/..."
RECOMMENDED SYSTEM REQUIREMENTS
Personal Computer
Operating System
Data Interface
Graphic Interface
Monitor Resolutions
Intel i7 Quad-Core (min. 3.0 GHz) processor | 8 GB RAM
Win 7 SP1 / Win 8.1 / Win 10 – 64 bit
MacOS El Capitan / Sierra – 64 bit
Linux Ubuntu 16.04 LTS – 64 bit
USB 3.0 integrated over PCI Express V2.0
Graphic card equipped with on-board memory
1920x1200 for SUBRA & KAPELLA & RIGEL
3840x2160 for ARKTUR & NAOS & PROKYON
MINIMUM SYSTEM REQUIREMENTS
Personal Computer
Operating System
Intel i5 (Dual-Core) processor or comparable | 4 GB RAM
Win 7 SP1/ Win 8.1 / Win 10 – 64 bit
MacOS El Capitan / Sierra– 64 bit
Linux Ubuntu 16.04 LTS – 64 bit
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Data Interface
Monitor Resolutions
USB 3.0 over PCI Express V1.1
1280x720 or higher
To re-use existing system environments without USB3.0 interface, we recommend installing USB 3.0
interface cards tested by Jenoptik (listed on the PROGRES GRYPHAX® price list):
-
USB 3.0 PCI-Express card for LAPTOP operation
USB 3.0 PCI-Express card for DESKTOP operation, including two brackets (standard bracket and
low profile bracket for mini-PC).
NOT SUPPORTED:
PROGRES GRYPHAX® cameras on USB2.0 interface! Connection issues/bad images can occur!
PROGRES GRYPHAX® cameras under Windows XP or 32Bit operating systems!
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23. Technical drawings & interfaces
Dimensions and interfaces of PROGRES GRYPHAX®
camera series
Technical drawings and interfaces of PROGRES GRYPHAX® cameras:
| 40*
* only valid for PROGRES GRYPHAX® PROKYON
PROGRES GRYPHAX® cameras are equipped with the following interfaces:
1.
Optical interface via c-mount – to connect the cameras to every microscope
2.
Electrical interface USB 3.0 – SuperSpeed (USB 3.1 Gen 1.) connection socket type micro-B – for data
communication and power supply. No additionall power supply is needed!
3.
Trigger connection socket – to connect an external device (trigger out signal is supported by camera)
4.
“ON / OFF” switch – to turn on or off the camera
5.
Status LED – to inform about the camera status as follows:
5.1. LED is permanently green – camera is connected to the computer and ready for operation
5.2. LED is blinking green – camera is delivering live image to the computer
5.3. LED is off – camera not connected to the computer or no power from USB interface
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24. Keyboard shortcuts
Keyboard Shortcuts of PROGRES GRYPHAX® software
PROGRES GRYPHAX® software is workflow-optimized and very easy to use. Therefore, GRYPHAX
software includes a lot of time-saving keyboard shortcuts.
Keyboard button:
Shortcuts for:
F1
Open help
F2 | ENTER*
Start capturing in “Capture Mode” (single shot, time-lapse, video)
F3
Return to “Live” mode
F5
Activates | deactivates live image freeze
F11
Switch between “Presentation view” and “Standard view”
DEL
Delete selected images from “Gallery” or marked drawings (measurement | annotation)
Z*
Switch zoom between “1:1” mode and “Fit to screen” mode
CTRL** + P
Print selected image(s) from “Gallery”
CTRL** + A
Select all (all thumbnails from “Gallery”)
CTRL** + G
Open | close “Gallery”
CTRL** + O*
Open “Storage Options” to change destination folders for gallery (images, videos)
CTRL** + T
Open | close “Toolbar”
CTRL** + D***
Open | close “Treeview” to change and manage media destination folder fast & easy
G
Open | close “Grid” view
ESC*
Leave “Presentation view” | remove drawings during creation (measurement | annotation)
Arrow up | down
Gallery thumbnail up | down
Page up | down
Gallery page up | down
Home
Jump to “Gallery” first page
End
Jump to “Gallery” last page
SPACE BAR
Activates “Magnifier” or “Hand tool”
*valid as of GRYPHAX version 1.0.14 | **for Mac users: CMD instead | ***valid as of GRYPHAX version 1.1.6
Hint: We all want to boost our productivity, so we recommend printing out or saving this list of keyboard
shortcuts to enhance user’s daily work.
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25. TWAIN / DirectX interface
User Guide for TWAIN / DirectX interface for
GRYPHAX® USB 3.0 cameras
The “TWAIN & DirectX driver” for PROGRES GRYPHAX® USB 3.0 cameras enables user to operate with
TWAIN and DirectX supported 3rd party software solutions. The driver delivers basic functionalities of
PROGRES GRYPHAX® cameras. Drivers are supported under Windows operating systems.
General description:
The GRYPHAX TWAIN & DirectX driver is part of PROGRES GRYPHAX® software installation (as of
version 1.1.10 or newer). The driver will be automatically installed and can be used without separate
configuration out of the box running a TWAIN or DirectX interface supporting application.
The driver consists of two separate windows. The preview window to display the live stream getting
from camera and the settings window, which contains all essential camera parameters.
The settings window is permanently visible, the preview window can be deactivated.
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25.1
Overview of settings window:
1. Toolbar – contains most important tools
2. Select active camera
3.



Format - change image settings for:
Live and Capture shot resolution
Fast capture mode
Option – Minimize gain during capture
4. Image parameter settings
5. Create / delete Shadings
6. Information field for help text & status
Note: All settings are saved (under folder: C:\Users\<user name>\AppData\Roaming\DijSDK\) for each
camera type and will be reused after open of driver again. Settings are not connected to individual
camera serial numbers.
25.2
Toolbar:
The Toolbar of TWAIN driver contains the following functions:

Save camera settings manually to currently used settings file.

Change between color mode and black / white mode for color cameras.

Start / Stop Live image preview at the preview window.

Shot button to start transfer single capture shot image via TWAIN interface.

Magnifier tool to activate / deactivate magnifier glass at preview window.

Save image option to save last displayed live image from preview window to hard drive.

White balance pipette to set manually white balance at preview window.
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Select camera:
To activate or change camera, select the appropriate camera from dropdown list of TWAIN driver.
After activation of camera
from dropdown list, the
according settings to the
camera type will be loaded
from settings file and used.
Note: In case of first camera start, the “Automatic setup” process will be proceed.
Format:
Under format section user can set-up different image resolution for live preview and capture shot.
“Fast capture mode” reduces processing time and transfer time for image transfer via TWAIN.
By activation of “Fast capture mode” the TWAIN driver will use the selected live resolution for single shot
(image transfer) instead of the selected capture shot resolution.
By activation of option “Minimize gain during capture” the gain value will be minimized for capturing
and adjusting the exposure time accordingly. This option is recommended and will reduce noise level
and enhance image quality.
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25.3
Camera image parameter:
Important: All camera image parameter are saved during close TWAIN driver for each camera type and
will be reused after open of TWAIN driver again.
The Automatic Setup option can be used at any time
to set all camera image parameter to default values. Additionally, the automatic white balance and the
color correction to the used light source will be applied and “Auto Exposure control” is enabled.
All camera image parameter can be adjusted by scroll slider or by enter of value.
The Exposure control option contains the following
parameter: Intensity value, Exposure time and Gain value.
Set Intensity level to activate the automatic exposure control function. The selected value corresponds
the mean intensity value in percent. Available range from 0 to 100%
Change exposure time value or gain value to activate manual exposure control.
Ocular View – to adapt the color impression from the eyepieces
(microscope) to the image on screen.
Contrast – to enhance image contrast for live and captured images.
Saturation – to change color saturation for live and captured images.
Gamma – to change gamma value for live and captured images.
Black Point – to enhance noise level in dark image areas.
Reset settings separately:
All camera image parameter can be reset to default value separately by pressing reset icon
Status bar information:
Status bar displays the connected camera, settings location, frame number and frame rate.
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.
Create / delete white shading:
White shading function corrects intensity variation across the image, caused by the microscope or
objectives.
Click on the button “White shading”
to create a white shading
correction. The TWAIN driver will automatically create a white shading correction file for the connected
camera type.
This will take up to some seconds, the progress indicator will
be shown during creation. Press “Esc” will abort process.
After successfully white shading creation the button will change status to “Reset White”
.
The white shading correction is now available and used. To deactivate the white shading press “Reset
White” and the white shading correction file will be deleted and cannot be reloaded again.
After reset / delete of shading file, a new shading file has to be created to activate shading again.
The shading files will be saved on: “C:\Users\<user name>\AppData\Roaming\DijSDK\” and are reused
after driver restart and appropriate camera type is connected.
Note: Shading files are connected to the camera type, not to the individual serial number!
Create / delete black shading:
Black shading function enhance noise level for long time exposed image.
Click on the button “Black shading”
to create a black shading
correction. The TWAIN driver will automatically create a black shading correction file for the connected
camera type.
This will take up to some minutes, the progress indicator will
be shown during creation. Press “Esc” will abort process.
After successfully black shading creation the button will change status to “Reset Black”
.
The black shading correction is now available and used. To deactivate the black shading press “Reset
Black” and the black shading correction file will be deleted and cannot be reloaded again.
After reset / delete of shading file, a new shading file has to be created to activate shading again.
The shading files will be saved on: “C:\Users\<user name>\AppData\Roaming\DijSDK\” and are reused
after driver restart and appropriate camera type is connected.
Note: Shading files are connected to the camera type, not to the individual serial number!
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25.4
Capture / Transfer images:
To transfer images by TWAIN interface press the “Shot” button
on the toolbar. The live preview
image will be stopped and a single image will be transferred by TWAIN interface to the application.
Live image will be restarted automatically at preview windows after transfer of image via TWAIN.
25.5
Start / Stop Live:
To stop or restart live preview at the preview window, press live control button
at the toolbar.
Open / close preview window:
To deactivate or activate the preview window of
driver, navigate to the “camera” menu entry
and choose “Show Preview”.
25.6
Preview window:
The preview window displays the live stream preview from the camera as well as the capture shot images.
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25.7
Preview window tools and options:
Preview window contains the following tools and information at the toolbar:
Image dimensions (X/Y) of current live preview
Display of current selected zoom level from preview window
Zoom slider or scroll wheel to change zoom level. Left position
zoom out, right position zoom in.
Image overview as overlay to display whole image in case of 1:1
view or image zoom over live preview.
Note: To change image section, use the rectangle from overview.
Display Mode drop down menu
to change between different view options:
“Normal view” – view image as color or b/w image without histogram
“Negative view” – invert image values to get negative image view
“Full plot” – to activate RGB and gray channel of histogram as overlay
“Red plot” – to activate red color channel of histogram as overlay
“Green plot” – to activate green color channel of histogram as overlay
“Blue plot” – to activate blue color channel of histogram as overlay
“Gray plot” – to activate gray channel of histogram as overlay
Fit to screen function activated by button or by left-hand mouse double click into
the preview window.
Fit to lens function to change back to 1:1 view and enable zoom slider.
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Display of RGB values and image coordinates of
corresponding mouse pointer position.
Presentation mode – to change between full screen and normal window by middle double click of
mouse on preview window. Whole live preview will be displayed on the screen.
Magnifier glass activated by Magnifier button
times on live preview.
to zoom in 4x
Note: By right-hand mouse click the magnifier changes between
rectangle and circle glass.
Limitations:




Performance and functionalities are limited due to used Microsoft TWAIN & DirectX interfaces.
Only GRYPHAX USB 3.0 cameras are support.
Multi-camera support is not available.
Windows OS (7 SP1 / 8.1 / 10) support only.
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26. USB 3.0 Camera Service Tool
USB 3.0 Camera Service Tool for PROGRES GRYPHAX®
cameras
The “USB 3.0 Camera Service Tool” enables user to check the installed camera firmware version and to
update the firmware version, if necessary. This service tool is part of the GRYPHAX® software installation
1.1.6 or later.
Additionally, the option "Dark blemishes" search for defective/ hot
pixel is available, to eliminate such pixel defects of the camera sensor.
Note: To operate with the USB 3.0 Camera Service Tool, the camera
driver has to be installed previously. Please connect only one camera
to the computer and start the service tool. Otherwise, USB 3.0
Camera Service Tool will be display an error message:
1. To start “USB 3.0 Camera Service Tool”, please navigate to start menu entry under:
Jenoptik/GRYPHAX 1.X.X
and double-click to:
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2.
Firmware update
2.1. After start of USB 3.0 Camera Service Tool the software will check the camera's firmware status and
verifies it with the firmware status from USB 3.0 Camera service tool. There are three different
update options in regard to the camera firmware:
No automatic firmware update
possible or necessary:
In case of accordance of the
firmware version, no automatic
firmware update is possible.
You now may close the USB 3.0
camera service tool by close
button.
Automatic firmware update (by
inbuilt firmware version):
Manual update by
selecting alternative
firmware file:
In case that the camera firmware
is outdated, please start firmware To upgrade or
update process by option "Start downgrade an
firmware update":
alternative camera
firmware version you
may use option
"Select alternative
firmware file…"
from
disk.
The
firmware version of
camera and file will
be
displayed
at
service tool. Now you
can start update by
option
"Start firmware
update"
Note: An alternative
firmware file will be
provided by Jenoptik
only!
2.2. After start of USB 3.0 Camera
Service Tool the software will
check the camera. After start
firmware update process, the
software is displaying the current
update status by progress bar.
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2.3. After a successful firmware update the USB 3.0
camera service tool will restart the camera and
close
software
automatically.
Note: The firmware update procedure can be take several seconds depending on connected
camera type. Please do not interrupt the process. This can be affect the camera.
3. Dark blemish search
In case that the camera is displaying white pixel defects at dark images, please proceed the Dark blemish
search to eliminate such “hot pixels”. The software will detect defective pixels and add these pixels to a
camera-internal list of defective pixels. So the application software will correct this automatically.
3.1. To start a dark blemish search procedure the sensor
must be covered by using the protection cap!
3.2. Start the dark blemish search by pressing “Start”. The
service tool will record several images to detect defective
pixel(s) from camera sensor. This can take several seconds
depending
on
the
camera
type
used.
Note: During dark blemish search, please do not remove
the cap from sensor, otherwise the software cannot
detect the defective pixels and the search process will be
aborted!
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3.3. After successful dark blemish search, the service
tool will add the new detected hot pixels to the
list of defective pixel.
3.4. If error “Image too bright” occurs, please check,
if the camera sensor is completely covered by the
protection cap.
Note: Make sure that no light falls onto the
sensor!
3.5. In case of error, restart the service tool and try to search for dark blemish pixel again.
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27.
Service & Support
1st level support
For questions about PROGRES GRYPHAX® microscope cameras, your local expert dealer from whom
you have purchased the PROGRES GRYPHAX® camera is the first contact partner. He is trained in using
the camera and can also give you information about accessories that are suitable for special microscopy
applications.
Manufacturer service and support
Furthermore, our local technical expert support in Jena can be contacted:
Daniel.Kaiser@jenoptik.com
28.
Return address & software updates
Return address in case of service incidents
Attn.: Mr. Steffen Derbsch
JENOPTIK Optical Systems GmbH
Pruessingstrasse 41
07745 Jena
Germany
In the unlikely case that a camera needs to be returned for inspection or repair, you do not need a RMA number.
The manufacturer data of each product is encoded into the product´s serial number. Contact the
support team to obtain the manufacture date of your specific product.
Camera registration and software updates
Users can register at our website:
https://www.jenoptik.com/en-progres-gryphax-download
Registered users are entitled to access the download area of our PROGRES GRYPHAX® website where
software updates and enhancements are provided regularly and free of charge.
Further information
More information about digital imaging products from JENOPTIK Optical Systems is available on our
website: www.jenoptik.com/progres-gryphax
Technical information
The technical specifications of your PROGRES GRYPHAX® camera are found on the GRYPHAX USB stick
in the folder “Data sheets“. Further technical information such as application reports, data sheets and
Frequently Asked Questions are available on our web site: www.jenoptik.com/progres-gryphax
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29.
Safety & Operating Instruction for PROGRES GRYPHAX Microscope Cameras
Intended Use
PROGRES GRYPHAX cameras are intended for the use on a microscope.
They can be adapted via c-mount adaption to the microscope and via USB3.0-cable to a computer or
laptop.
Take care to use only the USB3.0 cables included with your camera or explicitly recommended by Jenoptik.
Power Supply
For PROGRES GRYPHAX cameras, power supply and data communication are carried out using the
USB3.0 cable. Additional power supply connections are not necessary.
Contents
1. PROGRES GRYPHAX microscope camera
2. PROGRES GRYPHAX software - available: • on USB-memory card – item 5,
• to download at www.jenoptik.com/progres-gryphax
• on request by e-mail at progres@jenoptik.com
3. USB3.0 cable
4. Safety and Operating Instructions & Quick Start Guide
5. USB memory card containing user video tutorials & PROGRES GRYPHAX software
Trigger Operation
Some PROGRES GRYPHAX cameras support trigger operation (connection to an external trigger device,
which delivers the signal (Trigger In) to the camera and which will react, e.g. by capturing an image.
After image capture, the camera delivers a signal to the device (Trigger Out) to signal the completion of
the function. Trigger Out does not require any additional power supply; for Trigger In, power supply
must be set up for the external device. Connect the cable shield with the casing of the external device.
Only use shielded cables.
Conformity to CE / WEEE / ROHS / China RoHS
PROGRES GRYPHAX® microscope cameras comply with:
• CE in accordance with EMC Directive 2014/30/EU
• WEEE
• ROHS
• China RoHS
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Type Label
Note: Please observe the information on the type label when installing the camera.
The following information is printed on the type label: (e.g. PROGRES GRYPHAX SUBRA)
1.
2.
3.
4.
5.
Camera type:
Serial number (written and in code format):
Manufacturer’s website:
CE mark / WEEE mark / Voltage / Power:
Country of origin:
Cleaning and Maintenance
Cleaning the camera casing: If the camera casing is only slightly soiled, clean it with a soft, slightly
moistened piece of cloth. Make sure that no water enters the camera and risks becoming in contact
with any internal components.
Do not use any aggressive substances or solvents to clean your camera.
Cleaning the filter glass: Cleaning the filter glass by yourself is not recommended. If the filter glass is
severely soiled, please contact your expert dealer or the manufacturer for assistance.
Disclaimer
Exemption from warranty: Jenoptik shall be exempt from warranty during the warranty period in the
event that the safety regulations are not observed.
Exemption from statutory liability for accidents: Jenoptik shall be released from statutory liability for
accidents that occur in the event of non-observance of the safety instructions by any operating person.
Safety & Operating Instructions
PROGRES GRYPHAX cameras are intended for the use on a microscope and for operation and control
with PROGRES GRYPHAX microscopic imaging software. The PROGRES GRYPHAX microscope camera is
an optical and fine mechanical device. Please handle it with due care.
PROGRES GRYPHAX cameras should be used in clean and dry locations.
Every PROGRES GRYPHAX camera has been thoroughly tested and has left the factory in perfect
operating condition.
For your own safety and to keep the camera in good operating condition, please follow all
safety and operating instructions in this document and observe all advice and labels on the unit
and on any accessory.
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Expansions and alterations: The camera must be operated in compliance with these safety
instructions. Do not attempt to carry out any expansions, adjustments, alterations, or repairs by
yourself. Repairs and maintenance work may be carried out only by authorized service
personnel.
Electric installations: The electric installations of the room where the system is set up must be in
compliance with the IEC requirements.
Voltage supply: 5 V (USB) / Consumption: Variable according to camera type. Please refer to your
camera’s technical data sheet.
Unplug the USB cable to disconnect the camera from the power supply.
Use only cables included with your camera or explicitly recommended by Jenoptik.
• Make sure that the cables are installed so that they do not obstruct persons and do not cause a
tripping risk.
• Protect cables against mechanical impact or damage.
Note: Observe the information on the type label when installing the camera.
Caution, fire hazard! To prevent a risk of fire, do not operate or store the camera nearby easily
inflammable materials or gases.
Caution, risk of injury! Operating the camera under the following circumstances risks injury:
• The camera is visibly damaged
• The camera has been stored under adverse conditions over a long period of time
• The camera has been transported under adverse conditions
If any of these circumstances apply, switch off the camera and ensure that it cannot be operated
unintentionally. Please contact your expert dealer or the manufacturer’s technical support team for
assistance.
Caution, risk of damage by unsuitable environment conditions! Do not expose the camera to
extreme environment conditions. Avoid extremely high or low temperatures, and keep the
camera away from high humidity, liquids, chemical gases, dust or high electro-magnetic fields.
Caution, risk of injury or damage by water! If water (or other liquids) enters the camera, there is
a risk of electric shock. Your camera can also be damaged or no longer usable. If water has
entered the camera, switch it off and contact your expert dealer or the manufacturer for
assistance.
Caution, risk of damage by static charge! Static charge can damage or destroy the electronic
components of your camera. Before connecting the camera to a computer or a microscope,
make sure that it is free of electrostatic charge. Ground yourself by touching the metallic
housing or the reverse side of your computer or microscope, which both have to be grounded
via a power socket.
Caution, risk of malfunction by insufficient ventilation! Some PROGRES GRYPHAX cameras are
equipped with louvers on the rear side. Ensure that the cameras are sufficiently ventilated and
that the louvers are not covered.
Caution, risk of damage and malfunction by overheating! Avoid leaving your camera in direct
sunlight and do not operate the camera near heat sources (e.g., radiators or stoves).
Overheating can affect the image quality.
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Advice for handling the IR filter glass: Protect the integrated IR filter glass against mechanic
impact such as scratching or shock and against soiling. Avoid fingerprints on the glass and do
not touch the C-mount cover of the camera.
Caution, risk of damage and image errors by mechanic impact! Protect the camera against
impact, especially during operation. Mechanic impact can affect image quality.
Operating temperature:
+10 °C ... +35 °C
Relative humidity:
5 % ... 80 %, non-condensing
Storage and transportation temperature:
-20 °C ... +70 °C
Advice for transportation and storage: Protect the camera against impact. Store and transport the
camera in a dry and cool place, e.g., in its case or the packaging in which it was delivered. Please use
the supplied C-mount cover during transportation and storage.
Disposal
The camera must be disposed of in compliance with the environmental protection guidelines in
force. Contact your expert dealer in case of any questions.
The sign Caution warns against possible health dangers at risk if the advice is not observed.
The sign Attention warns against possible damage to the instrument.
The sign Information highlights important information for the operation of your camera.
This symbol highlights that special guidelines have to be followed when disposing of this product.
Learn more about PROGRES GRYPHAX® software and the easy workflows and tools.
VIDEO TUTORIALS
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Release date: 09/06/2017
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