AZURA Pump P 6.1L User Manual EN

Pump P 6.1L
User Manual
V6890
2
Note:
For your own safety, read the manual and always observe the warnings and
safety information on the device and in the manual
Manuel en français:
Si jamais vous préfériez un manuel en français pour ce produit, veuillez vous
contacter le support technique (Technical Support) par email ou par fax
avec le no. de série. Merci beaucoup.
Technical Support:
Publisher:
Version information:
Phone:
+49 30 809727-111 (9-17h, Central European Time)
Fax:
+49 30 8015010
E-Mail:
support@knauer.net
Languages: German, English
KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
D-14163 Berlin
Phone: +49 30 809727-0
Fax:
+49 30 8015010
Internet: www.knauer.net
E-Mail:
info@knauer.net
Article number: V6890
Version number: 2.2
Last update: 2017-02-16
Original edition
The information in this document is subject
to change without prior notice.
For latest version of the manual, check our website:
http://www.knauer.net/en/downloads.html
Copyright:
© KNAUER Wissenschaftliche Geräte GmbH 2017
All rights reserved.
® AZURA is a registered trademark of
KNAUER Wissenschaftliche Geräte GmbH.
3
Table of Contents
Table of Contents
Product Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Operating Ranges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Device Variants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Views . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Front View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Rear View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Performance Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6
6
6
6
8
8
9
Scope of Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Safety for Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Symbols and Signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Location Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Unpacking the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Capillaries and Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Connecting the Solvent Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Pre-Installed Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Integrating the Pump into an HPLC Flow System . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Isocratic pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Connecting the Pump Head to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Binary pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Connecting the Degasser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Connecting the Pump Head to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Connecting the Solvent Selection Valve to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . .
Quaternary Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Connecting the Degasser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Connecting the Pump Head to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Connecting the 4-Channel Degasser to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . .
Connecting the Valve Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Connecting the Piston Backflushing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Connecting the Leak Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Computer Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Configuring the LAN Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Configuring the Router . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Integrating the LAN into a Company Network . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Controlling Several Systems Separately in a LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Setting a Static IP Address . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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14
15
15
15
16
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17
18
18
18
19
19
20
20
21
21
22
23
23
23
24
24
25
25
25
Table of Contents
Pin Header Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Remote Terminal Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Events Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Connecting Cables to the Terminal Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Analog Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
4
26
26
27
29
30
30
Initial Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Bleeding the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Switch-On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
30
31
31
32
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Operating with Chromatography Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Operating with Mobile Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Meaning of the LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Functional Tests IQ and OQ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Possible Problems and Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
System Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Maintenance and Care. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Maintenance Contract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Maintenance Intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Cleaning and Caring for the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Checking the Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Flushing the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Putting the Pump Out of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Disconnecting the Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Preparing the Pump Head for Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Dismounting the Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Removing the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Cleaning the Check Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Installing the Check Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Inline Filter on the Pressure Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Removing the Inline Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Inserting the New Inline Filter Cartridge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Replacing the Mixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Removing a Leak . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
40
40
41
41
41
42
42
43
43
43
45
45
45
45
46
46
47
48
48
Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
P 6.1L General Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Main Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Communication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Table of Contents
Device Variants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
P 6.1L isocratic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
P 6.1L binary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
P 6.1L quaternary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Pump Heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
50
50
51
51
52
Repeat Orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Accessories and Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Legal Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Transport Damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Warranty Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Warranty Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
55
55
55
56
56
HPLC Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
P 6.1L user manual V6890
Product Information
6
Product Information
Note: Only use the device for applications that fall within the range of the
intended use. Otherwise, the protective and safety equipment of the device
could fail.
Description
The pump P 6.1L is suitable for analytical and semi-preparative applications.
Operating Ranges
Operating ranges
The device can be used in the following areas:
 biochemical analysis
 chemical analysis
 food analysis
 pharmaceutical analysis
 environmental analysis
Device Variants
The P 6.1L is available in three different options:
 Isocratic pump
 Binary pump (HDG pump)
 Quaternary Pump (NDG pump)
All variants are available as a standard version made of stainless steel or as a
bio-inert version made of ceramics with PEEK capillary, PEEK connectors,
and PEEK venting screw.
Views
Front View
Isocratic pump
The isocratic pump is equipped with a pressure sensor with an integrated
inline filter and a venting screw..
1
Legend
1 Pressure sensor
2
2 Pump head
Fig. 1
Isocratic pump - front view
P 6.1L user manual V6890
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Product Information
Binary pump
The binary pump consists of two pump drives and a 2-channel degasser
with a Solvent Selection valve. A pressure sensor with an integrated inline filter and a venting screw are also part of the pump.
1
Legend
1 Pressure sensor
2
3
4 5
2 Mixer
3 Pump head
4 Degasser
5 Solvent Selection
valve
Fig. 2
Quarternary pump
Binary pump - front view
The quaternary pump consists of a pump, a valve block and a 4-channeldegasser. A pressure sensor with an integrated inline filter, a venting screw,and
a mixer are also part of the pump.
1 2
Legend
1 Pressure sensor
3
2 mixing chamber
3 Pump head
4 Valve block
5 Degasser
Fig. 3
Binary pump - front view
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5
Product Information
8
Rear View
On the back of pump, the connectors for LAN and power chord are locatedas well as the serial number of the device an the power switch (on/off).
Service Interface
Please note that the service interface is solely used for repair and maintenance tasks performed by service technicians.
1
Legend
1 serial number
2 3
2 service interface
3 Pin header
4 LAN connector
5 Power connection
and power switch
4
Fig. 4
5
Rear view (example)
Performance Features
The pump is used to convey liquids, either in an HPLC system or as a standalone device. Liquids are conveyed either with constant flow or with constant pressure. The flow and pressure, which liquids can be conveyed with,
depend on the pump head.
Pump heads
Pump heads for several areas of application are available for the pump P
6.1L:
Standard
Bio-inert
Size
5 ml, 10 ml and 50 ml
10 ml and 50 ml
material
Pump head with stainless
steel inlays
Pump head with ceramic
inlays
Operating
Range
 HPLC
 Dosing applications
with non-aggressive
media
 Purification/FPLC
 Ion chromatography
Every pump head is equipped with an RFID chip. It is used to monitor and
save all important parameters and settings. The RFID technology offers the
following advantages:
 Software automatically recognizes the parameters of the pump head
 All service-relevant data of the pump head is stored
Mixer
Volume of the mixer is 100 μl. The mixer is exchangeable and can be
replaced by mixers with a volume of 50 μl or 200 μl .
The parameters necessary for the mixer are set via Mobile Control or via
chromatography software.
P 6.1L user manual V6890
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Product Information
Piston backflushing
Parameter
stainless steel
Bio-Inert
Size
50 μl, 100 μl, 200 μl
250 μl
Pressure
1000 bar
400 bar
The piston backflushing function automatically flushes the rear piston area
of the pump head upon switch-on and in continuous mode.
 Upon switch-on: The rear piston area of the pump head is automatically
flushed for 15 seconds.
 In continuous mode: The rear piston area of the pump head is flushed
automatically every 15 minutes, for 15 seconds.
PMax Mode
GLP Data
Optional accessories
This mode protects the column from excessive fluctuations in pressure. If
the maximum pressure is reached, the user can decide if the pump should
be switched off or continue running with constant pressure. You find a
detailed description on chromatography software in the corresponding user
manual.
The Mobile Control and the different software products (ClarityChrom® or
OpenLAB®) can be used to display or read GLP data. You find a detailed
description on how to display or read out GLP data in the respective user
manuals.
The following optional accessories are available:
 Pump head cooler for the pump head
 Mobile Control
Eluents
Even small quantities of other substances, such as additives, modifiers, or
salts can influence the durability of the materials.
If there is any doubt, contact the Technical Support of the manufacturer.
Unsuitable Eluents






halogenated hydrocarbons, e.g. Freon®
concentrated mineral and organic acids
concentrated bases
eluents containing particles
perfluorinated eluents, e.g. Fluorinert® FC-75, FC-40
perfluorinated polyether, e.g. Fomblin®
Less Suitable Eluents








diethylamine (0.1 %) (DEA)
dimethyl sulfoxide (DMSO)
slightly volatile eluents
methylene chloride1
tetrahydrofuran (THF)1
phosphoric dilute acid
triethylamine (0.1 %) (TEA)
trifluoroacetic acid (0.1 %) (TFA)
1. not recommended in combination with PEEK components or PEEK capillaries
P 6.1L user manual V6890
Product Information
Suitable Eluents
 acetate buffer solutions
 acetone at 4 °C-25 °C (39.2 °C-77.0 °F)1








acetonitrile2
benzene
chloroform
ethyl acetate
ethanol
formiate buffer solution
isopropanol
carbon dioxide (liquid 99.999 % CO2)







methanol
phosphate buffer solutions (0.5 M)
toluol
ammoniated dilute solution
acetic acid (0.1-1 %), at 25 °C
sodium hydroxide (1 M)
water
1. valid for the specified temperature range
2. not recommended in combination with PEEK components or PEEK capillaries
Unsuitable Eluents for degassers
















Azides
benzene
Hydro fluoro solvents
halogenated hydrocarbons, e.g. Freon®
heptane
Hexafluoroisopropanol (HFIP)
hexane
Hydrofluoric acid solutions
Carbon dioxide (liquid 99.999 % CO2)
concentrated mineral and organic acids
concentrated bases
eluents containing particles
perfluorinated eluents, e.g. Fluorinert® FC-75, FC-40
perfluorinated polyether, e.g. Fomblin®
Hydrochloric acid
dilute sodium hydroxide (1 M)
P 6.1L user manual V6890
10
11
Scope of Delivery
Scope of Delivery
Note: Only use original parts and accessories made by KNAUER or a company authorized by KNAUER.
 Pump P 6.1L
 Power cable
 AZURA accessories kit
 P 6.1L accessories kit
 User manual DE/EN
 Installation Qualification
Safety for Users
Professional Group
The user manual addresses persons who are qualified as chemical laboratory
technicians or have completed comparable vocational training.
The following knowledge is required:
 Fundamental knowledge of liquid chromatography
 Knowledge regarding substances that are suitable only to a limited
extent for use in liquid chromatography
 Knowledge regarding the health risks of chemicals
 Participation during an installation of a device or a training by the company KNAUER or an authorized company.
If you do not belong to this or a comparable professional group, you may
not perform the work described in this user manual under any circumstances. In this case, please contact your superior.
Safety Equipment
When working with the device, take measures according to lab regulations
and wear protective clothing:
 Safety glasses with side protection
 Protective gloves
 Lab coat
What must be taken into
account?
 All safety instructions in the user manual
 The environmental, installation, and connection specifications in the user
manual
 National and international regulations pertaining to laboratory work
 Original spare parts, tools, and solvents made or recommended by
KNAUER
 Good Laboratory Practice (GLP)
 Accident prevention regulations published by the accident insurance
companies for laboratory work
 Filtration of substances under analysis
 Use of inline filters
 Once they have been used, never re-use capillaries in other areas of the
HPLC system.
 Only use a given PEEK fitting for one specific port and never re-use it for
other ports. Always install new PEEK fittings on each separate port.
 Follow KNAUER or manufacturer’s instructions on caring for the colums.
P 6.1L user manual V6890
Safety for Users
12
More safety-relevant information is listed below:
 flammability: Organic solvents are highly flammable. Since capillaries can
detach from their screw fittings and allow solvent to escape, it is prohibited to have any open flames near the analytical system.
 solvent tray: Risk of electrical shock or short circuit if liquids get into the
device's interior. For this reason, place all bottles in a solvent tray.
 solvent lines: Install capillaries and tubing in such a way that liquids cannot get into the interior in case of a leak.
 leaks: Regularly check if any system components are leaking.
 power cable: Defective power cables are not to be used to connect the
device and the power supply system.
 self-ignition point: Only use eluents that have a self-ignition point higher
than 150 °C under normal ambient conditions.
 power strip: If several devices are connected to one power strip, always
consider the maximum power consumption of each device.
 power supply: Only connect devices to voltage sources, whose voltage
equals the device's voltage.
 toxicity: Organic eluents are toxic above a certain concentration. Ensure
that work areas are always well-ventilated! Wear protective gloves and
safety glasses when working on the device!
Where is use of the device
prohibited?
Never use the system in potentially explosive atmospheres without appropriate protective equipment. For further information, contact the Technical
Support of KNAUER.
Secure decommissioning
At any time, take the device completely out of operation by either switching
off the power switch or by pulling the power plug.
Opening the Device
The device may be opened by the KNAUER Technical Support or any company authorized by KNAUER only.
Signal Words
Possible dangers related to the device are divided into personal and material
damage in this user manual.
Lethal injuries will occur.
Serious or moderate injuries can occur.
Minor injuries can occur.
Device defects can occur.
Decontamination
Contamination of devices with toxic, infectious or radioactive substances
poses a hazard for all persons during operation, repair, sale, and disposal of
a device.
Life-threatening injuries
Health danger if getting in contact with toxic, infectious or radio-active substances.
 Before disposing of the device or sending it away for repair, you are
required to decontaminate the device in a technically correct manner.
All contaminated devices must be properly decontaminated by a specialist
company or the operating company before they can be recommissioned,
repaired, sold, or disposed of. All materials or fluids used for decontamination must be collected separately and disposed of properly.
P 6.1L user manual V6890
13
Symbols and Signs
Decontamination Report
Devices without a completed Decontamination Report will not be repaired.
If you would like to return a device to KNAUER, make sure to enclose a completed Decontamination Report with the device: http://www.knauer.net/
en/downloads/service.html
Symbols and Signs
The following symbols and signs can be found on the device, in the chromatography software or in the user manual:
Symbol
Meaning
Electric shock hazard
Note maximum weight-loading of the leak tray during
transport, installation and operation.
A device or system marked with CE fulfills the product
specific requirements of European directives. This is
confirmed in a Declaration of Conformity.
Testing seals in Canada and the USA at nationally recognized testing centers (NRTL). The certified device or system has successfully passed the quality and security
tests.
Installation
This chapter describes all preparatory steps prior to start-up. If you encounter difficulties during the installation, contact the Technical Support.
contact
Phone
+49 30 809727-111
Fax
+49 30 8015010
Mail
support@knauer.net
Transport
Carefully prepare the device for transport or storage. If you want to return
your device to KNAUER for repairs, enclose the Service Request Form which
can be downloaded from our website.
Device Data
For a secure transport, note the weight and dimensions of the device (see
Technical Data).
Bruising danger
Damage to the device by carrying or lifting it on protruding housing parts.
The device may fall and thus cause injuries.
 Lift the device only centrally on the side of the housing.
Lifting
Clasp the device at its side panels and lift it out of the packaging. Do not
hold onto front cover or leak tray.
P 6.1L user manual V6890
Installation
14
Location Requirements
Only if the requirements for ambient conditions of the operating environment are met, can the intended use be ensured. Details on the operating
conditions can be found in the Technical Data section.
Device defect
The device overheats at exposure to sunlight and insufficient air circulation.
Device failures are very likely.
 Set up the device in such a way that it is protected against exposure to
direct sunlight.
 Keep at least 15 cm clear at the rear and 5–10 cm at each side for air circulation.
Space Requirements




At least 5 cm if another device is set up on one side
At least 10 cm if further devices are set up on both sides
At least 15 cm on the rear panel for the fan.
Leave the power plug on the rear of the device accessible to be able to
disconnect the device from the mains.
General requirements




Position the device on a level and even surface.
Protect the device against direct exposure to sunlight.
Set up the device at a location not exposed to air drafts (A/C systems).
Do not set up the device in the vicinity of other machines that cause floor
vibrations.
Cooling room
It is possible to operate the pump in a cooling compartment when the followingrequirements are fulfilled:
 The pump is switched on.
 The pump is on standby modus.
Device defect
Changes of the environmental temperature cause condensation inside the
device.
 Allow device to acclimate for 3 h, before connecting to power supply and
taking into operation.
Earthquake Areas
If you are located in an earthquake area, use the bore holes 1 in the side
panels to secure the device. The bore holes are located on either right or left
side panel.
1
Fig. 5
bore holes of device
Unpacking the Device
Prerequisite
Check packaging for damage caused during transportation. If necessary, put
forward any claim for damages to the carrier.
P 6.1L user manual V6890
15
Installation
Tools
Utility knife
Bruising danger
Damage to the device by carrying or lifting it on protruding housing parts.
The device may fall and thus cause injuries.
 Lift the device only centrally on the side of the housing.
Process
Next steps
1. Set up the package in such a way that you can read the label. Using the
utility knife, cut the adhesive tape and open the packaging.
2. Remove the foam insert. Take out the accessory kit and the manual.
3. Open the accessory kit and check the scope of delivery. In case any parts
are missing, contact the Technical Support.
4. Clasp the device from below, lift it out of the packaging and place it on
its feet. Do not hold onto the front cover.
5. Check the device for signs of damage that occurred during transport. In
case you notice any damage, contact the Technical Support.
6. Place the device in its site of operation and remove protective foil.
Store packaging and keep the included packing list for repeat orders.
Capillaries and Fittings
All tubing and capillary, which connect the components of the pump, are
pre-installed. Only the solvent bottles have to be connected and the pump
has to be integrated into the flow of the HPLC system.
Connecting the Solvent Bottles
To connect the solvent bottles, tubing with pre-installed solvent filters is
used. The tubing is connected to the device with flangeless fittings.
Note: Do not use any tools to tighten the fittings.
Procedure
Process
Figure
1. Slide the flangeless fitting 1
over the tubing.
2. Slide the lock ring 2 over the
tubing, cap with the sealing ring
3. Note the direction of the
lock ring, otherwise the sealing
ring could be damaged. The
thicker end of the lock ring must
point into the direction of the
fitting.
1
Fig. 6
2 3
Set-up of a flangeless fitting
3. Manually fasten the flangeless
fitting 4 to the device.
4
Fig. 7
Fully assembled flangeless fitting
Pre-Installed Capillaries
Pre-installed stainless steel and PEEK capillaries are color-coded according to
their inner diameter.
P 6.1L user manual V6890
Installation
16
Color
Material
Inner Diameter
red marker
stainless steel
0.1 mm
blue marker
stainless steel
0.25 mm
black marker
stainless steel
0.45 mm
red stripe
PEEK
0.1 mm
blue stripes
PEEK
0.25 mm
orange stripes
PEEK
0.5 mm
Note: PEEK capillaries are not suitable for use with pure acetonitrile. Acetonitrile can cause capillaries to crack or rupture.
Integrating the Pump into an HPLC Flow System
The pump can be integrated into an HPLC flow system by connecting the
pressure sensor (isocratic version) or the mixer (binary or quaternary version) and the HPLC system with capillary.
Note: To integrate the pump into a system, note the ambient conditionsfound in the sections Operating Environment and Technical Data as well
asthe ambient conditions of other devices to be integrated into that system.
Device defect
Damage to the pump head caused by overtightened capillary fittings. Note
the torque of the fittings.
 Use 5 Nm torque for stainless-steel fittings.
 Use 1 Nm torque for PEEK fittings.
Tools
Torque wrench
Isocratic pump
The figure shows the installation plan for capillary and tubing on the isocratic version of the pump. If you own a different version of the pump, see the
corresponding chapter for installation instructions.
The gray lines represent the connection of the solvents to the pump and the
black lines represent the connection of the piston backflushing.
Capillary Layout
Fig. 8
Piston backflushing
Solvent flow path
Installation plan for the isocratic pump
The flushing solution is re-used. Since the flow path is circular, only one bottle is used for the flushing solution.
The pump head takes the liquid in from the bottle and conveys it to the
pressure sensor.
P 6.1L user manual V6890
17
Installation
Connecting the Pump Head to the Solvent
Prerequisites
material
 The device has been switched off.
 The power plug has been pulled.
 The front cover has been removed.
flangeless fitting
Device defect
The pump head can get damaged when the inlet and outlet are blocked.
 Remove the cap fittings from the inlet and outlet of the pump head prior
to use.
Procedure
Process
Figure
1. Slide the flangeless fitting over
the tubing.
2. Insert the tubing into the free
inlet 1 on the bottom of the
pump head.
3. Tighten the fitting by hand.
1
Fig. 9
Next Steps
Solvent line on pump head
Integrate the pump into the HPLC flow system.
Binary pump
The figure shows the installation plan for capillary and tubing on the binary
version of the pump. If you own a different version of the pump, see the
corresponding chapter for installation instructions.
The gray lines represent the connection of the solvents to the pump and the
black lines represent the connection of the piston backflushing.
Capillary Layout
1
3 2
Fig. 10
Piston backflushing
Installation plan for the binary pump
The flushing solution is re-used. Since the flow path is circular, only one bottle is used for the flushing solution.
P 6.1L user manual V6890
Installation
18
Every pump head can operate with two different solvents. Both solvents are
connected with the Solvent Selectionvalve 1. The solvents are conveyed
into one pump head each and combined in the pressure sensor. The pressure sensor is connected to the mixer. The mixer is connected to the HPLC
system.
Connecting the Degasser
The degasser inlet 2 is readily connected with the solvent selectionvalve 1.
The degasser outlet 3 is readily connected with the pump heads (see Fig.
10).
Solvent flow path
Both solvents are connected with the 2 degasser inlet. The solvents are
conveyed from the degasser into the pump head. From the pump head it is
taken via the pressure sensor to the mixer. The mixer is connected to the
HPLC system.
Note: The described flow path is not suitable for Normal Phases.
Connecting the Pump Head to the Solvent
Prerequisites
material
 The device has been switched off.
 The power plug has been pulled.
 The front cover has been removed.
flangeless fitting
Device defect
The pump head can get damaged when the inlet and outlet are blocked.
 Remove the cap fittings from the inlet and outlet of the pump head prior
to use.
Procedure
Process
Figure
1. Slide the flangeless fitting over
the tubing.
2. Insert the tubing into the free
inlet 1 on the bottom of the
pump head.
3. Tighten the fitting by hand.
1
Fig. 11
Next Steps
Solvent line on pump head
Integrate the pump into the HPLC flow system.
Connecting the Solvent Selection Valve to the Solvent
Note: The Solvent Selection valve is readily connected to the 2-channel
degasser.
The Solvent Selection valves allows selecting from two different solvents for
each solvent channel, without having to re-install the tubing. For both solvent channels A and B, one of two solvents can be selected. Solvent A is
connected to inlets A1 and A2, solvent B is connected to inlets B1 and B2.
P 6.1L user manual V6890
19
Installation
Prerequisites
material
Procedure
 The device has been switched off.
 The power plug has been pulled.
 The front cover has been removed.
Flangeless fitting
Process
Figure
1. Connect the tubing from the
four solvent bottles to the inlets
A1, A2 and B1, B2.
2. Seal inlets not in use with cap
fittings.
Fig. 12
Next Steps
Solvent Selection valve with
cap fitting
Integrate the pump into the HPLC flow system.
Quaternary Pump
The figure shows the installation plan for capillary and tubing on the quaternary version of the pump. If you own a different version of the pump, see
the corresponding chapter for installation instructions.
The gray lines represent the connection of the solvents to the pump and the
black lines represent the connection of the piston backflushing.
Capillary Layout
1
Fig. 13
Piston backflushing
2 3
Installation plan for the quaternary pump
The flushing solution is re-used. Since the flow path is circular, only one bottle is used for the flushing solution.
Connecting the Degasser
The degasser outlet 2 is readily connected to the valve block 1 (see fig.
13).
Solvent flow path
The four solvents are connected with the 3 degasser inlet. The solvent mixture is conveyed from the degasser via the valve block into the pump head.
From the pump head it is taken to the mixer. The mixer is connected to the
HPLC system.
Note: The described flow path is not suitable for Normal Phases.
P 6.1L user manual V6890
Installation
20
Connecting the Pump Head to the Solvent
Prerequisites
Material
 The device has been switched off.
 The power plug has been pulled.
 The front cover has been removed.
Flangeless fitting
Device defect
The pump head can get damaged when the inlet and outlet are blocked.
 Remove the cap fittings from the inlet and outlet of the pump head prior
to use.
Procedure
Process
Figure
1. Slide the flangeless fitting over
the tubing.
2. Insert the tubing into the free
inlet 1 on the bottom of the
pump head.
3. Tighten the fitting by hand.
1
Fig. 14
Next Steps
Solvent line on pump head
Integrate the pump into the HPLC flow system.
Connecting the 4-Channel Degasser to the Solvent
The 4-channel degasser contains four degassing chambers. Each degassing
chamber has an inlet and an outlet on the front of the pump.
Device defect
Very high pressures can damage the degasser membrane. The membrane
can withstand a maximum pressure of 7 bar.
 Never connect the degasser to the pump outlet.
Prerequisites
Material
 The device has been switched off.
 The power plug has been pulled.
 The front cover has been removed.
Flangeless fitting
P 6.1L user manual V6890
21
Installation
Procedure
Process
Figure
1. Connect the tubing from the
four solvent bottles to the inlets
A, B, C, and D of the degasser
(see 2).
2. Seal inlets not in use with cap
fittings.
1
Fig. 15
Next Steps
2
4-channel degasser with
valve block
Integrate the pump into the HPLC flow system.
Connecting the Valve Block
Note: Note the instructions on how to install the flangeless fitting (see
“Capillaries and Fittings” on page 15).
The valve block inlets 1 are pre-installed. If the central outlet connection
has to be changed, e.g. you want to install different capillary, note that at
least two of the outer flangeless fittings on the valve block are loosened in
order to be able to install connection in the center by hand.
Connecting the Piston Backflushing
The piston backflushing removes salts and other substances from the area
behind the pump head seals.
To do this, connect a bottle with flushing solution to the flush pump and
the piston backflushing. The silicone tubing between the piston backflushing and the flush pump are pre-installed.
Designation
Inlet and outlet of the flush pump are located on the front of the device. The
flush pump is inside of the device and not visible from the outside.
Fig. 16
Prerequisite
Material
Inlet symbol
Fig. 17
Outlet symbol
 The pump has been set-up at the site of operation.
 The pump has been switched off.
Silicone tube
P 6.1L user manual V6890
Installation
Procedure
Process
22
Figure
1 2
1. Plug one silicone tube 2 onto
the inlet 3 of the flush pump
and connect to the flushing
solution bottle.
2. Plug a second silicone tube onto
a vacant capillary connector1
of the flush pump and connect
to the flushing solution bottle.
3
Connecting the Leak Management
The leak management consists of the leak sensor and the drainage system
(funnels, hoses, nozzles). The drainage system ensures that escaping liquids
flow into a waste bottle. When leaks are registered by the leak sensor, the
LED flashes red. Both the device and the data acquisition via chromatography software are stopped.
Prerequisite
Process
The front cover has been removed.
Process
Figure
1. Carefully push the funnel 1 into
the center opening of the capillary guide 2.
1
Fig. 18
2
Funnel and capillary guide
3
2. Push the long ending of the first
nozzle 4 into the hose 3.
4
Fig. 19
Hose and nozzle
3. Connect the nozzle and the funnel.
4. Push the other end of the hose
onto the nozzle 5 of the leak
tray.
5
Fig. 20
P 6.1L user manual V6890
Hose connected to device
23
Installation
Process
Process
Figure
5. For the bottom device, push the
short end of the nozzle 6 into
the opening in the collection
point of the leak tray.
6. Connect the hose to the nozzle
and lead the second ending to
the waste bottle.
7. Place the waste bottle below the
bottom device.
Next steps
6
Fig. 21
Leak tray with nozzle
Attach the front cover.
Control
The pump can be operated in two ways:
 Via the pin head
 as part of a LAN, via the LAN connector of the router
All connectors for external control are located on the rear side of the pump.
1
Legend
1 Service interface
2 Events connector
3 LAN connector
4 Remote connector
2
3
4
Fig. 22
Connectors on rear side
Computer Control
This section describes how to set up an HPLC system in a local area network
(LAN) and how a network administrator can integrate this LAN into your
company network. The description applies to the operating system Windows and all conventional routers.
Note: To set up a LAN, we recommend to use a router. That means the following steps are required:
Process
1.
2.
3.
4.
5.
On the computer, go to the control panel and check the LAN properties.
Hook up the router to the devices and the computer.
On the computer, configure the router to set up the network.
Install the chromatography software from the data storage device.
Switch on the device and run the chromatography software.
Configuring the LAN Settings
The LAN uses only one server (which is normally the router) from that the
devices automatically receive their IP address.
P 6.1L user manual V6890
Installation
24
Prerequisite
 In Windows, power saving, hibernation, standby, and screen saver must
be deactived.
 In case you use an USB-to-COM box, the option "Allow the computer to
turn off ths device to save power" in the devicemanager must be deactivated for all USB hosts.
 Only for Windows 7: For the network adapter, the option "Allow the
computer to turn off this device to save power" in the Device Manager
must be deactivated.
Process
1. In Windows 7 choose Start  Control Panel  Network and Sharing Center.
2. Double-click on LAN Connection.
3. Click on the button Properties.
4. Select Internet Protocol version 4 (TCP/IPv4).
5. Click on the button Properties.
6. Check the settings in the tab General. The correct settings for the DHCP
client are:
a) Obtain IP address automatically
b) Obtain DNS server address automatically
7. Click on the button OK.
Connecting the Cables
A router 3 has several LAN ports 2 and one WAN port 4 that can be used
to integrate the LAN into a wide area network (WAN), e.g. a company network or the Internet. In contrast, the LAN ports serve to set up a network
from devices 1 and a computer 5. To avoid interference, we recommend
operating the HPLC system separately from the company network.
1
2
3
4
5
You will find patch cables for each device and the router in the accessories
kit. To connect the router to a WAN, an additional patch cable is required,
which is not supplied within the scope of delivery.
Prerequisite
Process
 The computer has been switched off.
 There is a patch cable for each device and the computer.
1. Use the patch cable to connect the router and the computer. Repeat this
step to connect all devices.
2. Use the power supply to connect the router to the mains power system.
Configuring the Router
The router is preset at the factory. You will find a label at the bottom side of
the router, on which IP address, user name, and password are printed.
These information help to open the router configuration.
Process
1. To open the router configuration, start your Internet browser and enter
the IP address (not for all routers).
2. Enter user name and password.
3. Configure the router as DHCP server.
P 6.1L user manual V6890
25
Installation
4. In the router configuration, check the IP address range and make
changes if necessary.
Result
Once the router has assigned IP addresses to all devices, the chromatography software can be used to remotely control the system.
Integrating the LAN into a Company Network
A network administrator can integrate the LAN into your company network.
In this case you use the WAN port of the router.
Prerequisite
Process
There is a patch cable for the connection.
1. Check that the IP address range of the router and of the company network do not overlap.
2. In case of an overlap, change the IP address range of the router.
3. Use the patch cable to connect the router WAN port to the company network.
4. Restart all devices, including the computer.
Controlling Several Systems Separately in a LAN
Devices connected to a LAN communicate through ports, which are part of
the IP address. If more than one HPLC system is connected to the same LAN
and you plan on controlling them separately, you can use different ports to
avoid interference. Therefore, the port number for each device must be
changed and this same number must be entered into the device configuration of the chromatography software. We recommend to use the same port
number for all devices in the same system.
Note: The port is set to 10001 at the factory. You must use the same numbers in the device configuration of the chromatography software as in the
device, otherwise the connection fails.
Process
Result
1. Find out port number and change it on the device.
2. Enter the port number in the chromatography software.
The connection is established.
Setting a Static IP Address
Note: Please inform yourself about the IT safety standards valid for your laboratory, before changing the LAN settings.
The device is factory set to a dynamic IP address (DHCP). To ensure a permanent LAN connection between the chromatography software and the
device, we recommend to set a static IP address for certain applications.
Prerequisites
Procedure
 The device has been switched on.
 Mobile Control has been installed and started.
 The connection between the Mobile Control and the device has been
established.
Note: You find further information on LAN settings in the chapter Device
Settings of the Mobile Control User Manual.
1. In the Mobile Control, choose Settings
.
2. On the General tab, choose the device name.
3. Under Network Settings, choose the setting Static 1.
P 6.1L user manual V6890
Installation
26
1
2
3
4. Enter the IP address into the text box IP Address 2.
5. If necessary, change the subnet mask and the gateway 3.
6. Click
Next steps
in the top right corner.
7. Restart the device.
In case necessary, go back to the original setting with the [Reset] button that
can be found under Settings > General > Network Settings > LAN Settings.
Pin Header Connectors
Remote Terminal Strip
 For receiving start, control, and error signals from external devices
 For sending start, control and error signals to external devices
Legend
1 display
2 Remote terminal strip
1
2
Fig. 23
Remote terminal strip
Signal
Explanation
Analog GND
Reference point of the voltage at the signal inputs.
Analog out
Voltage range 0 – 5 V, scalable
Analog in
Voltage range 0 – 10 V
10 V according to maximum flow rate
Start GND
Reference point of the voltage at the signal inputs.
P 6.1L user manual V6890
27
Installation
Signal
Explanation
Start OUT
TTL Output
Levels:
 passive 5 V
 active 0 V
Start IN
TTL Input
 Low active
Secure switching threshold at least 10 mA
After receiving a signal (short-circuit to ground)
from an external device, the device starts. If controlled with software, an electronic trigger is send
through the LAN.
Error OUT
TTL Output
Levels:
 passive 5 V
 active 0 V
Error IN
TTL Input
 Low active
Secure switching threshold at least 10 mA
After receiving a signal (short-circuit to ground)
from an external device, an error message appears
and the device stops.
Error GND
Reference point of the voltage at the signal inputs.
+24V
Event-controlled switching of 24 V
against GND
Protection: 24 V-200 mA
+5V
Provides a voltage of 5 V with respect to GND.
This makes it possible to supply a consumer that is
switched by an EVENT.
Protection: 5 V–50 mA
GND
Reference point of the voltage at the signal inputs.
Events Connector
For test purposes or in some other cases, it can make sense to manually
enter these signals:
 sending control signals (Events) to external devices
 opening and closing contacts
 Activating 500 ms pulses
P 6.1L user manual V6890
Installation
28
n
Legend
1 Display
2 Events terminal strip
1
2
Fig. 24
Assignment
Events Remote Connector
connection
Function
1TTL
TTL Output
Levels:
 passive 0 V
 active 5 V
Pulse:
 5 V for at least 1000 ms
2TTL
TTL Output
Levels:
 passive 0 V
 active 5 V
Pulse:
 5 V for at least 1000 ms
3OC
TTL-compatible output
Levels:
 passive 5 V
 active 0 V
Pulse:
 5 V for at least 1000 ms
GND
Reference point of the voltage at the signal inputs.
4TTL
TTL Output
Levels:
 passive 0 V
 active 5 V
Pulse:
 5 V for at least 1000 ms
P 6.1L user manual V6890
29
Installation
Assignment
connection
Function
5TTL
TTL Output
Levels:
 passive 0 V
 active 5 V
Pulse:
 5 V for at least 1000 ms
6OC
TTL-compatible output
Levels:
 passive 5 V
 active 0 V
Pulse:
 5 V for at least 1000 ms
GND
Reference point of the voltage at the signal inputs.
Relay Contact
The contact is on a floating basis. Its setting
depends on the settings in the software.
Steady-rate signal:
 passive = open relay contact
 active = closed relay contact
Pulse:
 Closed relay contact for at least 1000 ms
Permissible load of the relay contact: 1 A/
24 V DC
Relay Contact
The contact is on a floating basis. Its setting
depends on the settings in the software.
Steady-rate signal:
 passive = open relay contact
 active = closed relay contact
Pulse:
 Closed relay contact for at least 1000 ms
Permissible load of the relay contact: 1 A/
24 V DC
Connecting Cables to the Terminal Strip
To control one device through another, you use the multi-pin connector. To
use remote control, you have to connect cables to the terminal strip (both
included with delivery). The single ports are used to exchange control signals.
Prerequisite
Tools
 The device has been turned off.
 The power plug has been pulled.
Depressor tool
Electronic defect
Connecting cables to the multi-pin connector of a switched on device
causes a short circuit.
 Turn off the device before connecting cables.
 Pull the power plug.
P 6.1L user manual V6890
Initial Start-Up
30
Electronic defect
Electrostatic discharge can destroy the electronics.
 Wear a protective bracelet against electrostatic discharge and ground.
Process
1. Push the depressor tool 1into an
upper small opening on the front
of the terminal strip 3.
2. Lead the cable into the opening
2 below the inserted depressor
tool.
3. Remove the depressor tool.
1
2
3
Next steps
Check if the cables are firmly attached. Push the terminal strip onto the
multi-pin connector. Finish the installation. Then put the device into operation.
Analog Control
Analog ports serve for exchanging analog control signals. Reference point
for the signals is the connector GND.
 OUT: Device sends signal.
 IN: Device receives signal.
Power Connection
Prerequisites
Note: The nominal capacity of the connected devices must be maximum
50 % of the power supply to account for larger inrush currents when
switching on the modules.
The maximum power input is 100 W.
 The electrical power supply at the installation site must be connected
directly to the nearest main power line.
 The power must be free from ripple, residual current, voltage peaks and
electromagnetic interference.
 The connectors for the mains voltage are grounded accordingly.
 The device receives sufficient power with reserve capacity
The device is intended for use with AC power networks of 100-240 V.
Power Cable
Only the supplied power cable is to be used to connect the device to the
power supply. Replace defective power cables only with accessories from
KNAUER. Only use power cables with a permission for use in your country.
Power Plug
Make sure that the power plug on rear of the device is always accessible, so
that the device can be disconnected from the power supply.
Next Steps
Finish the installation and connect the device to the mains.
Initial Start-Up
Note: Prior to switching on the pump, you should bleed it to remove air
from capillary and tubing.
Start-Up
Use this checklist to check if the detector is ready for initial start-up:
 Device is positioned in the correct location.
 The power plug has been connected.
P 6.1L user manual V6890
31
Initial Start-Up
If the device is part of an HPLC system, you should also note the following:
 The network connection to the router is established
 The chromatography software has been installed by KNAUER or a company authorized by KNAUER.
 The capillaries have been connected.
Pump Head
To bring a newly installed pump head into service securely, it should run in
prior to its first operation. For this purpose, set the following parameters:
Note: If pump heads are used for normal phase applications, only use isopropanol as solvent.
Pump head 5 ml, 10 ml
Pump head 50 ml
H2O (degassed) or ethanol
H2O (degassed) or ethanol
30 MPa (min > 15 MPa)
12 MPa (min > 8 MPa)
Flow rate
2-5 ml/min
5-20 ml/min
Run time
30 min
90 min
Capillary with inner diameter of
0.1 mm
length 2 m
Capillary with inner diameter of
0.1 mm
length 1 m
Eluent
Back pressure
Drag
Back pressures higher than 8 MPa are generated by back pressure regulators.
Bleeding the Pump
Before the pump can be used, it must be bleeded.
Mode “Flow on”
Quick suction process
Prerequisites
Tools
The pump can only be bled when switched on because the syringe can only
take in liquid in "Flow on" mode. Only then are the valves of a binary pump
opened.
For the binary pump, the bleeding process may take a while during initial
start-up because the solvent tubes are filled with liquid for the first time.
 The installation has been completed.
 The capillaries and tubings have been connected.
 The pump has been switched on.
Syringe with Luer lock
Column defect
Damage to the column due to bleeding.
 Open the venting screw.
 Remove the column.
P 6.1L user manual V6890
Operation
Procedure
Process
32
Figure
1. Open the venting screw 1of the
pressure sensor.
2. With the syringe, extract fluid
through the bleed port 2.
1
2
Fig. 25
Venting screw of the pressure
sensor
Next Steps
Start the pump using the software (option: Purge) with a medium flow rate
(50 % of the maximum flow). If the extracted fluid flows continuously, stop
suction and close the venting screw.
Result
After bleeding, excess air is removed from the pump head and the capillary.
Switch-On
Note: Prior to switching on the pump, you should bleed it to remove air
from capillary and tubing.
After switching on the pump, the piston backflushing automatically flushes
for 15 seconds.
Prerequisites
The installation has been completed.
Device defect
Changes of the environmental temperature cause condensation inside the
device.
 Allow device to acclimate for 3 h, before connecting to power supply and
taking into operation.
Component defect
Damage to the pump head in case it runs dry.
 Ensure that liquid runs through pump head and piston backflushing.
Procedure
Next Steps
1.
2.
3.
4.
Connect the power cable of the device to the power supply.
Switch the power supply on.
Wait until the pump has completed the self-test.
Start the pump at a medium flow rate.
Operate the pump.
Operation
There are several options for controlling the device:
 with chromatography software
 with Mobile Control
Note: It is not possible to use 2 control methods simultaneously. If the
device is connected to the software, it cannot be controlled via Mobile Control.
Operating with Chromatography Software
To operate the device with software, you have to establish a connection
between the LAN port and a computer.
P 6.1L user manual V6890
33
Operation
Devices can be controlled with e. g. OpenLAB EZChrom edition version
A.04.05 or higher or ClarityChrom version 5.0.2 or higher. You find a
detailed description on chromatography software in a corresponding user
manual.
Operating with Mobile Control
The Mobile Control is an app which can be installed on your computer or
tablet. To control the device using the Mobile Control, connect the computer or tablet with operating system Windows 8 to a wireless LAN router.
The firmware version of your P 6.1L must be V01.02 or higher. You find a
detailed description on the Mobile Control in its accompanying user manual.
Meaning of the LEDs
On the LED panel there are three LEDs 1 and a standby button 2. The figure shows the LED panel when the device is switched off.
1
2
The LEDs can have different colors depending on the operating conditions.
Left LED
Center LED
Right LED, Power Status
Standby
Color
Operating condition
Operation
red flashing
Error status
Check the system.
Shortly press the
switch to deactivate
the error message.
red
Fatal error
Restart the device.
If the fatal error status
remains, contact Service.
green
Program or sequence
is running/was loaded.
does not light
Not ready
green flashing
Equilibration
green
Ready
green
Power on
blue
Standby
Wait until the device is
ready.
Press the switch to end
the standby.
To activate the standby, keep the switch pressed for 5 seconds.
Note: Malfunctioning system after repeated standby possible. After repeatedly using the standby, switch off the power switch and back on again, to
reset the data storage.
P 6.1L user manual V6890
Functional Tests IQ and OQ
34
Functional Tests IQ and OQ
Installation Qualification
(IQ)
Operation Qualification
(OQ)
Test Intervals
Execution
The customer may request the Installation Qualification, which is free of
charge. In case of a request, the Technical Support of KNAUER or from a
provider authorized by KNAUER performs this functionality test during the
installation.
The Installation Qualification is a standardized document that comes as part
of the delivery and includes the following:
 confirmation of flawless condition at delivery
 check if the delivery is complete
 certification on the functionality of the device
The Operation Qualification includes an extensive functionality test according to KNAUER standard OQ documents. The Operation Qualification is a
standardized document and free of charge. It is not part of the delivery,
please contact the Technical Support in case of request.
The Operation Qualification includes the following:
 definition of customer requirements and acceptance terms
 documentation on device specifications
 device functionality check at installation site
To make sure that the device operates within the specified range, you
should test the device regularly. The test intervals are dependent on the
usage of the device.
The test can be carried out either by the Technical Support of KNAUER or
from a provider authorized by KNAUER (for a fee).
P 6.1L user manual V6890
35
Troubleshooting
Troubleshooting
First Measures
1.
2.
3.
4.
5.
Check all cabling.
Check all screw fittings.
Check whether air has gotten into the supply lines.
Check device for leaks.
Pay attention to system messages.
LAN
Go through the following steps, in case no connection between the computer and the devices can be established. Check after each step if the problem is solved. If the problem cannot be located, call the Technical Support.
1. Check the status of the LAN connection in the Windows task bar:

Connected

Connection not established
If no connection was established, test the following:
 Is the router switched on?
 Is the patch cable connected correctly to the router and the computer?
2. Check the router settings:
 Is the router set to DCHP server?
 Is the IP address range sufficient for all the connected devices?
3. Check all connections:
 Are the patch cable connected to the LAN ports and not the WAN
port?
 Are all cable connections between devices and router correct?
 Are the cables plugged in tightly?
4. If the router is integrated into a company network, pull out the patch cable from
the WAN port.
 Can the devices communicate with the computer, even though the
router is disconnected from the company network?
5. Turn off all devices, router, and computer. Firstly, turn on the router and secondly turn on the devices and the computer.
 Has this been successful?
6. Replace the patch cable to the device with that no connection could be established.
 Has this been successful?
7. Make sure that the IP port of the device matches the port in the chromatography software.
Possible Problems and Solutions
Error
Solution
Device cannot be
switched on
Inspect the power cable to ensure that it is
plugged into the power supply.
When purging, the
pump switches off.
Check if the venting screw on the pressure sensor
is turned up.
P 6.1L user manual V6890
Troubleshooting
36
Pump does not
transport solvent
 Purge the pump head to remove the air bubbles.
 Clean the check valves.
 Exchange the check valves.
 If the pump head seals are defective, solvent
enters the piston backflushing; inform the
technical support.
 Exchange the pump head
Pressure and flow
rate variations
 Purge the pump head to remove the air bubbles.
 Always tighten the inlet screw fittings and outlet screw fittings on the pump head with a
torque wrench (see ’Checking the Fittings’ auf
Seite 41.).
 Clean the check valves.
 Exchange the check valves.
 Exchange the pump head
 Inform technical support.
Pump head leaks
 Inspect the inlet and outlet screw fittings of the
pump head.
 If the seals are defective, eluent enters the piston backflushing; inform the technical support.
 Exchange the pump head
Flow rate is not correct
Check the following options:
 Check the data for the solvent compressibility
 Clean the check valves.
 Exchange the check valves.
System error
Switch off the device to reset the device's data
storage and restart it afterwards.
System Messages
If other system messages are displayed besides those listed below, please
turn the device off and then on. Inform the technical support in case the
system message repeats.
The system messages are in alphabetical order:
System message
A
"A line with this time
already exists: edit
the time please"
Correct the time entry.
C
"Cannot delete
active program/link"
Pause the program/link. Only then can the link
and, subsequently, the program used in the link
be deleted.
"Cannot edit program from the running link"
Pause the link. Afterwards, the program can be
changed.
“Cannot initialize
LAN”
Check cables and connections in local area network.
P 6.1L user manual V6890
37
Troubleshooting
System message
"Cannot operate
with an empty link"
Create a link.
"Cannot purge
during the run"
End method and start purging.
"Cannot read data
from FRAM"
Switch the device off and on. Inform the technical
support in case the system message repeats.
"Cannot start time
table"
Check the data on the device's display or in the
opened program.
"Cannot use nonexisting component"
Check if the channels to be mixed are set correctly
in the Setup section of the device or in the Instrument Setup of the chromatography software.
"Cannot write data
on FRAM"
Switch the device off and on. Inform the Technical
Support in case the system message repeats itself.
"Component settings not compatible
with gradient setup"
Change the setup settings or change the gradient
in the program or in setup.
E
”Error input activated”
Eliminate the short circuit connected to ‘Error In’.
F
"Flow max in the
program is not compatible with the current pump head"
When entering the flow, note the maximum flow
of the pump head and only enter values inside
this range.
G
"GUI communication failed"
Switch the device off and on. Inform the technical
support in case the system message repeats itself.
H
"HPG B component
not present"
"HPG B: Command
timeout”
"HPG B: incompatible pump head
type"
"HPG B: Service
active"
I
”Instrument remote
controlled”
This entry is not executable. Quit software.
"Invalid index in
time table"
Change the entry in the program line.
"Invalid line number"
L
"Invalid link"
Create a link or use an existing link.
"Leak sensor not
present"
Switch the device off and then on.
If the leak sensor cannot be found, contact the
Technical Support.
P 6.1L user manual V6890
Troubleshooting
38
System message
M
“Leak was detected”
Switch off the device. Remove the leak and start
the device afterwards.
"Line in time table is
empty"
Edit the program line.
"Link is running"
Wait until the link has been completed, then
change the link or delete it.
"Maximum pressure:
System stopped"
 Check whether the connected capillary and
connectors are clogged.
 Adjust the maximum pressure limit.
 Restart the system.
"Minimum pressure:
System stopped"
 Increase the pressure or adjust the lower pressure limit.
 Restart the system.
"Motor failure:
max current"
Switch the device off and on. Inform the technical
support in case the system message repeats itself.
"Motor failure:
position error"
"Motor failure:
N
"No link available.
Pls edit link first"
Create a link and edit it.
"No link available"
P
"No valid pump
head type detected;
50 ml pump head is
set."
 Switch the device off and on.
 Check whether a pump head with RFID recognition has been installed.
 Repeat the automatic configuration step in the
chromatography software.
 Remove pump head, clean it and install it
again.
"Non-existing component is set to non0 value"
Switch on the channel or edit the data using the
chromatography software.
"Not enough space
to store link"
 Check the pump
 Check the number of program lines - a maximum of 100 program lines are possible.
"Not enough space
to store program"
 Check the pump
 Check the number of program lines - a maximum of 100 program lines are possible.
"Not in HPG mode"
Select HPG mode.
"Pressure max in the
program is not compatible with the current pump head"
Note the maximum pressure of the pump head.
P 6.1L user manual V6890
39
Troubleshooting
System message
"Program does not
exist, please edit the
program number"
Create and edit a program.
"Program is running"
Quit program or wait until program has been
completed.
"Pump head type:
head data uninitialized"
 Switch the device off and on
 Check whether a pump head with RFID recognition has been installed
 Repeat the automatic configuration step in the
chromatography software
 Remove pump head, clean it and install it again
"Pump head type:
read failed"
 Switch the device off and on
 Repeat the automatic configuration step in the
chromatography software
 Remove pump head, clean it and install it again
 Inform the Technical Support in case the system message repeats
"Pump head type:
RFID hardware not
present or failed"
Pump head without RFID detection: If necessary,
replace pump head.
"Pump head type:
write failed"
 Switch the device off and on
 Repeat the automatic configuration step in the
chromatography software
 Remove pump head, clean it and install it again
 Inform the Technical Support in case the system message repeats
S
"SetPoint in the program is not compatible with the current
PH PMax"
Note the maximum pressure of the pump head.
T
"This link is used in
WAKEUP"
First quit or delete wakeup program (wu = Wake
Up), then edit or delete link.
"This program is
used in a link"
First pause or delete the link, then edit or delete
data by means of the chromatography software.
"This program is
used in WAKEUP"
First quit or delete wakeup program (wu = Wake
Up), then edit or delete data by means of the
chromatography software.
"Too many lines in
program"
Check the number of program lines.
A maximum of 100 program lines are possible.
"Unable to attain
min. flow setpoint"
Confirm, pump continues running
"Unable to attain
pressure setpoint"
The entered pressure cannot be achieved with the
maximum flow set in the Constant Pressure mode.
 Check for leaks.
 Increase the upper flow level.
 Reduce the working pressure.
U
P 6.1L user manual V6890
Maintenance and Care
40
System message
W
"Unknown pump
head type"
 Check the pump head.
 Check whether a pump head with RFID recognition has been installed
 Inform the Technical Support in case the system message repeats
"Wake up time
already passed"
Correct the entry for date or otherwise time.
Maintenance and Care
Organic eluents are toxic above a certain concentration. Ensure that work
areas are always well-ventilated! When performing maintenance tasks on
the device, always wear safety glasses with side protection, protective
gloves, and an overall.
All wetter components of a device, e. g. flow cells of detectors or pump
heads and pressure sensors for pumps, have to be flushed with a suitable
flushing solution before being maintained, disassembled or disposed.
Opening the Module
The device may only be opened by the KNAUER Technical Support or any
company authorized by KNAUER.
Electronic defect
Performing maintenance tasks on a switched on device can cause damage
to the device.
 Switch off the device
 Pull the power plug.
Device defect
Leaks can damage the device.
 If leaks occur after maintenance or assembly, replace the capillary connections with new ones.
Users may perform the following maintenance tasks themselves:
 Replacing the pump head
 Replacing the check valves of the pumps
 Replacing the inline filter of the pressure sensor
 Replacing the mixer
Proper maintenance of your HPLC device will ensure successful analyses and
reproducible results.
Maintenance Contract
The following maintenance work on the device may only be performed by
KNAUER or a company authorized by KNAUER and is covered by a separate
maintenance contract:
 Opening the device or removing housing parts
Maintenance Intervals
Operating Hours
The Control Unit, the Mobile Control and the different software products
(e.g. ClarityChrom® or OpenLAB®) enable you to display or read out the
operating time of the pump. You find a detailed description on how to display or read out GLP data in the respective user manuals.
P 6.1L user manual V6890
41
Maintenance and Care
Operating Hours
Measures
1000
 Check the torque of the screw fittings
 Clean the pistons of the pump
 Check the check valves of the pump head
5000
 Replace all seals
 Clean the check valves of the pump head
10000
 Replace the spare parts in the pump head
 Replace the check valves of the pump head
Cleaning and Caring for the Device
Device defect
Intruding liquids can cause damage to the device.
 Place solvent bottles next to the device or in a solvent tray.
 Moisten the cleaning cloth only slightly.
All smooth surfaces of the device can be cleaned with a mild, commercially
available cleaning solution, or with isopropanol.
Checking the Fittings
Check if all fittings are tight. In case you find fittings that are not tight,
tighten them up. Not the torque applicable for each fitting in order to not
damage any components.
Material & Size
Pump head inlet
Pump head outlet
Stainless steel 5 ml
7.5 Nm
10 Nm
Stainless steel 10 ml,
50 ml
7.5 Nm
7.5 Nm
Inline Filter Fittings
Torque
Stainless steel fittings
7.5 Nm
PEEK fittings
3.5 Nm
Capillary Screw Fittings
Torque
Stainless-steel fittings
5 Nm
PEEK fittings
1 Nm
Flushing the Pump
Generally, the pump and all its components (valves, degasser) should be
flushed after each operation. Also, flush the pump to clean the tubing
before changing the solvent and to remove air bubbles in the capillary and
tubing. If buffer solution has been employed, note that the buffer solution
and the flushing solution are compatible.
 When buffers have been used, flush with water.
 When aggressive solvents have been used, flush with isopropanol.
Note: For applications wirth Normal Phase, only use Isopropanol as flushing
solution.
P 6.1L user manual V6890
Maintenance and Care
42
Device defect
Residuals chemicals can damage the pump head if they are not being
removed before storage.
 For reuse, flush the pump head and fill it with isopropanol.
 Before storage, seal the inlets and outlets with cap fittings.
Auxiliary Material
Procedure
flushing solution, silicone tubing
Process
Figure
1. Immerse the solvent tubing into
the flushing solution.
2. Plug a silicone tube onto the
venting nozzle 2 of the pressure
sensor.
1
2
3. Open the venting screw 1.
4. Start the pump at a medium
flow rate.
Fig. 26
Next Steps
Pressure sensor
Bring the pump into operation again.
Putting the Pump Out of Operation
The pump is designed for the use of different solvents. In case the pump has
not been used for several weeks, solvent residues may cause damage. It is
therefore recommended that all components of the pump be flushed, the
solvent used be removed completely and that all components and tubes of
the pump be filled with isopropanol. All open connectors should be sealed.
Capillary and tubing which connects single components of the pump
should not be removed.
Prerequisites
Tools
Procedure
 The pump has been rinsed.
 The pump has been switched off and disconnected from the power supply.
Open-end wrench, size 10
Process
1. Remove the solvent tubing and seal all open connectors with cap fittings.
2. Remove the pump from the HPLC flow system and seal the open connector of the pressure sensor (isocratic version) or the mixer (binary or
quaternary version) with cap fittings.
Next Steps
Select a storage location according to the requirements, which are listed in
the according chapter of this manual.
Storage
Pay attention that all hoses and capillaries have been emptied or filled with
flushing solution (e. g. isopropanol) before storage. To prevent algae formation, do not use pure water. Close all inputs and outputs with cap fittings.
P 6.1L user manual V6890
43
Maintenance and Care
The device can be stored under the following ambient conditions:
 temperature range
4-40 °C (39.2-104 °F)
 air humidity
below 90 %, non-condensing
Disconnecting the Power Supply
Prerequisites
Procedure
The device has been switched off.
Process
1. Pull the power plug out of the socket and afterwards out of the device.
2. Pack the power cable together with the device.
Next Steps
Disconnect all remaining electrical connections, remove the pump head and
pack the device for transport or storage.
Preparing the Pump Head for Storage
Prerequisites
Auxiliary Material
The power plug has been pulled.
 Syringe
 Flushing solution
Device defect
Residuals chemicals can damage the pump head if they are not being
removed before storage.
 For reuse, flush the pump head and fill it with isopropanol.
 Before storage, seal the inlets and outlets with cap fittings.
Procedure
Process
1. Fill the flushing solution into a syringe and inject into the capillary at
the pump head inlet. Leave it for 5 minutes.
2. Flush with suitable flushing solution.
3. Flush the pump head with isopropanol.
4. Seal the inlets and outles with cap fittings.
Next Steps
Dismount the pump head.
Dismounting the Pump Head
Prerequisites
Tools
 The pump head has been purged.
 The tubes at the inlet and outlet have been removed.
 The pump head's inlet and outlet have been sealed with cap fittings.
 Allen wrench 3 mm
 Open-end wrench, size 1/4"
 Open-end wrench, size 13
Chemical burns
Aggressive or toxic solvent residue can irritate the skin.
 Wear protective gloves.
 Flush the pump head before exchanging it.
P 6.1L user manual V6890
Maintenance and Care
44
Device defect
Residuals chemicals can damage the pump head if they are not being
removed before storage.
 For reuse, flush the pump head and fill it with isopropanol.
 Before storage, seal the inlets and outlets with cap fittings.
Component defect
Damage to the pistons if they tilt.
 Undo or tighten diagonally opposite screws evenly by one turn each at a
time.
Procedure
Process
Figure
1. Loosen the capillary connectors
1 at the outlet. Remove the
capillary.
1. Fixate the outlet fitting 1 with
an open-end wrench (size 13).
2. Loosen the fitting 2 with an
open-end wrench (size 1/4").
1
2
Fig. 27
Outlet screw fitting
1
3. Remove the solvent lines 2
from the solvent inlets.
4. Disconnect the hoses of the piston backflushing 1 from the
flush pump and the pump head.
2
Fig. 28
5. Using the Allen wrench, unscrew
the 4 screws 1 subsequently by
one turn.
6. Fixate the pump head with your
hand and remove the screws.
7. Lift off the pump head.
1
Fig. 29
P 6.1L user manual V6890
Tubings piston backflushing
Screws
45
Maintenance and Care
Next Steps
Maintain the check valves or install a new pump head.
Check Valves
Clogged check valves do not open and close properly. They cause pressure
fluctuations and irregular flow. If it is impossible to clean the check valves,
replace the whole unit.
Procedure
 Removing the Check Valves
 Cleaning the Check valve
 Installing the Check Valve
Removing the Check Valves
The pump head is equipped with two check valves.
Prerequisites
Tools
Procedure
 The pump head has been purged.
 The capillaries and tubings have been removed.
 The pump head has been removed.
 Open-end wrench, size 13
Process
Figure
1
1. Unscrew and remove the capillary connector 1.
2. Loosen the outlet fitting 2 with
the open-end wrench..
3. Remove the first check valve.
2
4. Loosen the inlet fitting 3 with
the open-end wrench..
5. Remove the second check valve.
3
Fig. 30
Next Steps
Check valve in pump head
Clean the check valves.
Cleaning the Check Valve
The check valves are not disassembled for cleaning but they are cleaned as a
unit.
Prerequisites
Procedure
Both check valves have been removed.
Process
1. Place each check valve into a beaker with solvent, e.g. isopropanol.
2. Put the beaker with the check valve in an ultrasonic bath for at least 10
minutes.
3. Let the check valves dry afterwards.
Next Steps
Insert both cleaned check valves.
Installing the Check Valve
Insert the check valves in the direction of the flow. The notch of the check
valve points downward.
P 6.1L user manual V6890
Maintenance and Care
Normal phase
Prerequisites
46
Insert the check valves in the direction of the flow. The arrow on the check
valve points upward.
The check valves have dried.
Component defect
Damaging the threads of components caused by overtightened fittings. Pay
attention to the torque values.
 Use 5 Nm for stainless steel fittings.
 Use 1 Nm for PEEK fittings.
Procedure
Process
Figure
1. Insert the check valves 1 in
such a way that the notch of the
check valve 2 points downward.
2. Manually screw in inlet and outlet fittings and tighten them
with a torque wrench and the
respective torque.
Next Steps
1
2
Fig. 31
Check valve
Re-install the pump head.
Inline Filter on the Pressure Sensor
Clogged inline filters inside of the pressure sensor can cause pressure fluctuations and irregular flow. Inline filters are not cleaned but exchanged as an
assembly.
Procedure
1. Remove the capillary below the inline filter of the pressure sensor.
2. Loosen the fitting of the inline filter and remove manually.
3. Insert a new inline filter cartridge.
Removing the Inline Filter
Below the pressure sensor, you find the fitting of the inline filter.
Prerequisites
Tools
Procedure
The pump has been rinsed.
 Open-end wrench, size 1/4"
 Open-end wrench, size 13
Process
Figure
1. Fixate the outlet fitting 2 with
an open-end wrench (size 13).
1
2. Loosen the fitting 3 below the
inline filter 2 of the pressure
sensor 1 with the open-end
wrench.
2
3
Fig. 32
P 6.1L user manual V6890
Loosen the capillary.
47
Maintenance and Care
Procedure
Process
Figure
3. Loosen the fitting of the inline
filter 1 with the open-end
wrench (size 13) and removemanually.
4. Remove clogged inline filter cartridge.
1
Fig. 33
Next Steps
Loosen the screw fitting of
the inline filter cartridge
Insert the new inline filter cartridge.
Inserting the New Inline Filter Cartridge.
The flow direction is designated on inline filter cartridges. The inline filter
and fitting are inserted into the pressure sensor in such a way that the designating notch always points upwards.
Tools
 Torque wrench
Component defect
Damaging the threads of components caused by overtightened fittings. Pay
attention to the torque values.
 Use 5 Nm for stainless steel fittings.
 Use 1 Nm for PEEK fittings.
Procedure
Process
Figure
1. Insert the inline filter cartridge
with the designating notch
pointing upwards 1 into the
fitting 2 .
2. Manually, screw the fitting with
the inline filter cartridge in the
pressure sensor.
3. Using the torque wrench,
tighten the fittings with the
appropriate torque.
1
2
Fig. 34
Next Steps
Inserting the inline filter cartridge into the fitting
Reinstall the capillary at the pressure sensor below the fitting of the inline filter.
P 6.1L user manual V6890
Maintenance and Care
48
Replacing the Mixer
A clogged mixer can cause pressure fluctuations and irregular flow. The
mixer is replaced completely as an assembly.
Voraussetzung
Werkzeuge
 The mixer has been rinsed with isopropanol.
 The sealing plugs are on site.
 Open-end wrench, size 1/4”
 Allen wrench, size 2 mm
Ablauf
Abbildung
1. Remove capillary 1at the inlet
and outlet of the mixer by hand
or with the open-end wrench.
1. Seal the inlet and the outlet with
the sealing plug.
2. Remove the screws
Allen wrench.
2 with the
3. Detach the mixer and set aside.
4. Install new mixer.
1
2
Fig. 35
HPLC Mixer
1
5. Manually, screw the fittings of
the capillary in the mixer.
6. Tighten with a torque wrench
using the appropriate torque.
2
Fig. 36
Nächste Schritte
Bio Mixer
 Bring the device into operation again.
Removing a Leak
Prerequisites
Auxiliary Material
Procedure
If liquid enters the inside the device, switch off the device. If this is not the
case, it is not necessary to switch off the device.
cloth for drying the leak sensor
Process
1. Remove the leak.
2. Dry the leak tray.
3. Acknowledge the system error via Mobile Control, or the standby button (only in standalone mode).
Next Steps
Afterwards take the device into operation.
P 6.1L user manual V6890
49
Technical Data
Technical Data
P 6.1L General Data
Main Features
Solvent conveyance
Degasser module
Variants
Isocratic HPLC pump
Quaternary low-pressure gradient pump
Binary high-pressure gradient pump
Delivery system
Dual-piston pump
Pulsation compensation
Active pressure and pulsation compensation
Pulsation
< 2 % Amplitude (typically: < 1,3 %) or
< 3 bar (0,3 MPa), whatever is greater, at 1
ml/min ethanol,
at all pressures > 10 bar (1 MPa, 147 psi)
Flow rate range
0,001–10 ml/min
0,02–10 ml/min (recommended)
0,01–50 ml/min
0,1–40 ml/min (recommended)
Flow rate increment
0.001 ml/min
Flow rate accuracy
< 1 % (measured at 5-80 % of flow range,
using ethanol)
Flow rate precision
0.1 % RSD (based on the retention time at
constant room temperature)
Flushing piston seal
Standard
System protection
Soft start, Pmin und Pmax are programmable
Wetted materials
Stainless steel, carbon-fiber-reinforced PTFE,
FKM, PEEK, saphire, aluminum oxide
(Al2O3)
Degasser channels
2 channels, Teflon® AF
Degasser max. flow rate
10 ml/min
Degasser method
Gas permeation using Teflon® AF amorphous fluoropolymer membrane
Degasser efficiency
< 0.5 ppm dissolved O2 at 1 ml/min
Degassing chamber volume
480 μl volume per channel
eluents
Limitations: hydrochloric acid and halogenated hydrocarbons, in particular hexafluoroisopropanol (HFIP)
Wetted materials
PEEK, Tefzel®, Teflon® AF
Vacuum chamber
Polypropylene and stainless steel
Vacuum pump
Low hysteresis
P 6.1L user manual V6890
Technical Data
50
Communication
Interfaces
 LAN
 Pin header connectors (Analog IN, Start
IN, Error IN)
Control
 LAN
 Analog and event control
 Mobile Control
Analog input
0-10 V
Analog control input
Flow rate
Level / event outputs
8 event outputs (TTL, OC, Relais) and 24 V
programming
19 programs, 9 program links, 1 WAKE UP
program
GLP
RFID pump head detection, detailed report
display
3 LEDs
leak sensor
Yes
protection type
IP-20
General
Power supply
Power input: 100-240 V
Output: 50-60 Hz
Maximum power consumption: 100 Watt
dimensions (W × H × D)
361 mm x 208.2 mm x 523 mm
weight
(see ’Device Variants’ auf Seite 50.)
Leak sensor
yes
temperature range
4-40 °C (39.2-104 °F)
air humidity
below 90 %, non-condensing
Device Variants
P 6.1L isocratic
Setup
Weight
Pump type
Isocratic analytical HPLC pump
Pump Head Versions
5 ml/min stainless steel
10 ml/min stainless steel
50 ml/min stainless steel
10 ml/min ceramic
50 ml/min ceramic
Delay volume
60 μl (at 10 ml/min)
weight
11.5 kg
P 6.1L user manual V6890
51
Technical Data
P 6.1L binary
Setup
Weight
Gradient formation
Mixer
Pump type
Binary analytical HPLC pump with degasser
Pump Head Versions
5 ml/min stainless steel
10 ml/min stainless steel
10 ml/min ceramic
10 ml/min for stainless steel
for Normal Phase applications
degasser
2 channels
Solvent selection valve
2 x 2 channels
weight
14.1 kg
Gradient type
High-pressure gradient
Gradient range
0–100 %
5–95 % (recommended)
Minimum increment
0.1 %.
Gradient precision
± 0,3 % (measured at 1 ml/min, 150 bar,
tracer: ethanol/caffeine)
± 1 % (5–95 %, measured at 0.1-10 ml/
min, tracer: water/caffeine)
Gradient repeat accuracy
< 0.1 % RSD (measured at 1 ml/min, 0.3 %
RSD overall, based on retention time at constantroom temperature)
Mixing volume
50, 100, 200 μl;
250 μl (metal-free)
Delay volume
110 μl (dependent of mixer),
410 μl (metal-free)
P 6.1L quaternary
Setup
Weight
Gradient formation
Pump type
Quaternary analytical HPLC pump with
degasser
Pump Head Versions
5 ml/min stainless steel
10 ml/min stainless steel
10 ml/min ceramic
degasser
4 channels, Teflon® AF
Special feature
Automatic adaption of LPG cycle time
weight
12.7 kg
Gradient type
Low-pressure gradient
Gradient range
0–100 %
1-99 % (recommended)
Minimum increment
0.1 %.
P 6.1L user manual V6890
Repeat Orders
Mixer
52
Gradient precision
± 0,3 % (measured at 1 ml/min, 150 bar,
tracer: ethanol/caffeine)
± 2 % (1-99 %, measured at 5-50% of the
flow range, tracer: water/caffeine)
Gradient repeat accuracy
< 0.1 % RSD (measured at 1 ml/min, 0.5 %
RSD overall, based on retention time at constantroom temperature)
Mixing volume
50, 100, 200 μl;
250 μl (metal-free)
Delay volume
210 μl (dependent of mixer),
410 μl (metal-free)
Pump Heads
Flow rate range
Maximum pressure
5 ml pump head
0.001 ml/min-2 ml/min
10 ml pump head
0.001 ml/min-10 ml/min
0.02-10 ml/min (recommended).
50 ml pump head
0.001 ml/min-50 ml/min
0.01-40 ml/min (recommended).
5 ml pump head
 1000 bar (100 MPa, 14504 psi) up to
2 ml/min
 700 bar (70 MPa, 10150 psi)
10 ml pump head
stainless steel
 700 bar (70 MPa, 10150 psi) up to 5 ml/
min
 400 bar (40 MPa, 5800 psi)
Ceramic
 400 bar (40 MPa, 5800 psi)
50 ml pump head
200 bar (20 MPa, 2900 psi)
Repeat Orders
Further Information
This list for repeat orders is valid for the time the document has been published. Deviations afterwards are possible.
Note: For repeat orders of spare parts use the enclosed packing list. Contact
the Technical Support in case there are any questions on spare parts or
accessories.
Further information on spare parts and accessories can be found online:
www.knauer.net
Devices
Name
Order number
P 6.1L isocratic with 10 ml stainless steel pump head
APH30EA
P 6.1L isocratic with 10 ml stainless steel pump head for
Normal Phase
APH30ED
P 6.1L isocratic with 50 ml stainless steel pump head
APH30FA
P 6.1L user manual V6890
53
Repeat Orders
Name
Order number
P 6.1L isocratic with 50 ml stainless steel pump head for
Normal Phase
APH30FD
P 6.1L isocratic with 10 ml ceramic pump head
APH60EB
P 6.1L isocratic with 50 ml ceramic pump head
APH60FB
P 6.1L binary with 10 ml stainless steel pump head,
degasser and mixer (100 μl)
APH35EA
P 6.1L binary with 10 ml stainless steel pump head for
Normal Phase, degasser and mixer (100 μl)
APH35ED
P 6.1L binary with 5 ml stainless steel pump head and
mixer (100 μl)
APH35GA
P 6.1L binary with 10 ml stainless steel pump head and
mixer (100 μl)
APH38EA
P 6.1L binary with 10 ml stainless steel pump head for
Normal Phase and mixer (100 μl)
APH38ED
P 6.1L binary with 50 ml stainless steel pump head and
mixer (200 μl)
APH38FA
P 6.1L binary with 10 ml ceramic pump head, degasser
and mixer (250 μl)
APH65EB
P 6.1L binary with 10 ml ceramic pump head, degasser
and mixer (250 μl)
APH68EB
P 6.1L binary with 50 ml ceramic pump head, degasser
and mixer (250 μl)
APH68FB
P 6.1L quaternary with 10 ml stainless steel pump head,
degasser and mixer (100 μl)
APH34EA
P 6.1L quaternary with 5 ml stainless steel pump head,
degasser and mixer (100 μl)
APH34GA
P 6.1L quaternary with 10 ml stainless steel pump head
and mixer (100 μl)
APH39EA
P 6.1L quaternary with 10 ml ceramic pump head,
degasser and mixer (250 μl)
APH64EB
P 6.1L quaternary with 50 ml ceramic pump head,
degasser and mixer (250 μl)
APH69EB
Accessories and Spare Parts
Pump head
Name
Order number
Pump head, 5 ml, stainless steel
AHA60
Pump head, 10 ml, stainless steel
AHB40
Pump head, 10 ml, ceramic
AHB32
Pump head 10 ml, stainless steel for Normal Phase
AHB40BA
P 6.1L user manual V6890
Repeat Orders
Check valves
Inline filter cartridge
Mixer
Solvent tray
Drainage System
Capillary guide
Mobile Control
Accessories Kit
Tools
Product riser
54
Name
Order number
Pump head, 50 ml, stainless steel
AHC20
Pump head 50 ml, stainless steel for Normal Phase
AHC20BA
Pump head, 50 ml, ceramic
AHC22
Check valve unit for AZURA 5, 10 ml pump head, ruby
sapphire
G0924B
Check valve unit for AZURA 5, 10 ml pump head, normal phase, spring-supported, ruby sapphire
G0563-5
Check valve unit for AZURA 50 ml pump head, ruby
sapphire
G0924C
Filter cartridge, stainless steel, 2 μm (20 μl volume)
A96601
Filter cartridge, titanium, 2 μm (60 μl volume)
A9661
AZURA mixer 50 μl
AZZ00MB
AZURA mixer 100 μl
AZZ00MC
AZURA mixer 200 μl
AZZ00MD
AZURA mixer 250 μl, bio
AZZ10ME
AZURA solvent tray E 2.1L
AZC00
corrugated hose, 16 cm, PE grey
A9846-1
corrugated hose, 150 cm, PE grey
A9846-3
funnel
P6431
exhaust
P6432
capillary guide top
P6424
Capillary guide side
P6425
Mobile Control license with 10” touchscreen
A9607
Mobile Control Chrom license with 10” touchscreen
A9608
Mobile Control license
A9610
Mobile Control Chrom license
A9612
AZURA accessories kit
FZA02
Accessories kit P 6.1L isocratic
FPH30
Accessories kit P 6.1L quaternary
FPH34
Accessories kit P 6.1L binary
FPH35
AZURA tool kit
A1033
Capillary cleaning kit
A0137
Metal capillary cutter
A0681
AZURA product riser (28 mm)
A9860
P 6.1L user manual V6890
55
Legal Information
Power cable
Documents
Name
Order number
USA
M1651
UK
M1278
Switzerland
M1597
Europe
M1642
User Manual Pump P 6.1L
V6890
Installation Qualification Document
VIQ_INST
Operation Qualification Document
VOQ_PUMPS
Legal Information
Transport Damage
The packaging of our devices provides the best possible protection against
transport damage. Check the devices for signs of transport damage. In case
you notice damages, contact the Technical Support and the forwarder company within three workdays.
Warranty Conditions
The factory warranty for the device is stipulated by contract. During the
warranty period, any components with material or design-related defects
will be replaced or repaired by the manufacturer free of charge. Please connect to our website for further information on terms and conditions.
All warranty claims shall expire in the event that any unauthorized changes
are made to the device. This warranty also excludes the following:
 accidental or willful damage
 damage or errors caused by third parties that are not contractually
related to the manufacturer at the time the damage occurs
 wear parts, fuses, glass parts, columns, light sources, cuvettes and other
optical components
 damage caused by negligence or improper operation of the device and
damage caused by clogged capillary
 packaging and transport damage
In the event of device malfunctions, directly contact the manufacturer.
KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
14163 Berlin, Germany
Phone:
+49 30 809727-111
Telefax: +49 30 8015010
e-mail:
info@knauer.net
Internet: www.knauer.net
Declaration of Conformity
The Declaration of Conformity is part of the delivery and accompanies the
product as a separate document.
P 6.1L user manual V6890
HPLC Glossary
56
Warranty Seal
The warranty seal is color-coded. A blue seal is used by the assembly or
technical support of KNAUER for devices to be sold. After repair, service
technicians affix an orange seal in identical position. If unauthorized persons
interfere with the device or the seal is damaged, the warranty claim will forfeit.
Disposal
Hand in old devices or disassembled old components at a certified waste
facility, where they will be disposed of properly.
AVV Marking in Germany
According to the German "Abfallverzeichnisverordnung" (AVV) (January,
2001), old devices manufactured by KNAUER are marked as waste electrical
and electronic equipment: 160214.
WEEE Registration
KNAUER as a company is registered by the WEEE number DE 34642789 in
the German "Elektroaltgeräteregister" (EAR). The number belongs to category 8 and 9, which, among others, comprise laboratory equipment.
All distributors and importers are responsible for the disposal of old devices,
as defined by the WEEE directive. End-users can send their old devices manufactured by KNAUER back to the distributor, the importer, or the company
free of charge, but would be charged for the disposal.
Solvents and Other Operating Materials
All solvents and other operating materials must be collected separately and
disposed of properly.
All wetted components of a device, e. g. flow cells of detectors or pump
heads and pressure sensors for pumps, have to be flushed first with isopropanol and then with water before being maintained, disassembled or disposed.
HPLC Glossary
Here you find definitions for abbreviations and technical terms, which are
used in liquid chromatography.
Term
Definition
Binary pump
Pump configured to generate a gradient from two solvents.
Chromatogram
Record of a detector signal, depending on output volume of mobile
phase and time
Degasser
Degasser module for fluids
Detector
device measuring the composition or the quantity of a substance.
Pressure sensor
Component for measuring the system pressure
Eluent
mobile phase transporting substances to be separated or isolated through
the column
Flangeless fitting
Fitting without a protruding edge, which can be fastened manually.
GLP
Good Laboratory Practice, quality assurance system for laboratories
P 6.1L user manual V6890
57
HPLC Glossary
Term
Definition
Gradient
Time-dependent composition of solvent (mobile phase) on low-pressure
or high-pressure side of system
LPG pump
Pump configured to generate a gradient on the high-pressure side. Usually a binary pump with two separate pump units which each convey one
solvent. The gradient is generated behind the pump.
High-pressure side
Devices and capillary which are under high pressure in an HPLC system
HPLC
High-Pressure Liquid Chromatography (HPLC) High pressure liquid chromatography
Isocratic
Mode of sample separation where the composition of a solvent remains
constant
Capillary
thin metal or PEEK pipe that connects components and devices within the
chromatography system
Check valve
Valve unit built into the pump head which guides the flow of liquid from
the inlet to the outlet.
LAN
Local area network (LAN). Network in which HPLC devices and a computer are connected, in order to control the devices.
LED
Light-emitting diode
Solvent
mobile solvent transporting substances to be separated or isolated
through the column
Ground
Protective measure for electroconductive housing parts
Mixer
Component for the homogenous mixing of solvents for the generation of
a gradient
Mobile Control
Mobile application to control and program the whole HPLC system via
wireless LAN.
NPG pump
Pump configured to generate a gradient on the low-pressure side. Only
one pump unit is used which contains small doses of the different solvents. The solvents are mixed in small mixing cycles by a valve block
which is connected upstream. The gradient is generated behind the
pump.
Low-pressure side
Devices and capillary which are under high pressure in an HPLC system
pH
Measure for the acidic or alkaline character of an aqueous solution.
Pump
Device which conveys solvents in controlled quantities into a system.
Quaternary pump
Pump configured to generate a gradient from four solvents.
Column
The column is filled with a matrix which separates the single components
of a substance spatially by interacting with an analyte transported with
the solvent to different degrees.
Solvent selection valve
Component of the pump that allows selecting from two different solvents
for every solvent channel. With this mechanism the solvent can be
changed without having to manually change the system.
Dead volume
Volume of capillaries and system components between mixing chamber,
injector and column as well as between column and detector.
Valve
device to diversify the eluent flow
P 6.1L user manual V6890
Index
Index
A
H
additives 9
ambient conditions 14, 43
Analog port 30
AVV marking 56
High-pressure side 57
HPLC 8
B
Binary pump 6, 7, 16, 17
C
Cap fitting 17, 18, 20
capillary 57
fitting 15
pre-installed 15
Check valve 45
cleaning 45
installing 45
removing 45
chromatogram 56
clean 41
Computer
control 23
contamination 12
Control 23
D
dead volume 57
decontamination 12
Degasser
2-channel 7
4-channel 7, 20
degassing chamber 20
Eluent list 10
dimensions 50
disposal 12
E
Electrical connections
Remote terminal strip 26
eluents 9
error 33
F
Fitting
flangeless 15, 56
fitting 15
Fittings
checking 41
FPLC 8
front cover 15
G
GLP data 9
I
Inline Filter
Removing 46
Inline filter cartridge
inserting. 47
reorder 54
installation
site 14
IQ 34
Isocratic pump 6, 16
L
label 15
LAN 23
port 25
problems 35
router 24
settings 23
setup 24
troubleshooting 35
leak 12
leak management 22
leak sensor 22
LED 33
Low-pressure side 57
M
maintenance
contract 40
intervals 40
Mixer
bio-inert 9
replacing 48
Mixing chamber
reorder 54
mixing chamber 8
modifiers 9
O
operating environment 14
Operating Hours 40
operating ranges 6
OQ 34
P
packing list 15
pin header 26
Piston backflushing 9, 44
Connecting 21
P 6.1L user manual V6890
58
59
PMax Mode 9
port (LAN) 25
power
disconnect 14
plug 14
power cable 12
power strip 12
power supply 12
connect 30
Pressure sensor 6, 7
professional group 11
Pump
bio-inert 6
bleeding 31
Flushing 41
Integrating into System 16
Pump head
bio-inert 9
connecting 20
dismounting 43
storing 43
pump head
reorder 53
Putting the Pump Out of Operation 42
Q
Quarternary pump 7, 16
Quaternary Pump 19
Quaternary pump 6, 57
R
remote connector
pin header 27
repair 12, 13
RFID chip 8
router (LAN) 24
S
safety equipment 11
salts 9
scope of delivery 15
Index
Screw fittings
torque 41
Service Interface 8
software 8, 22
solvent
flammability 12
line 12
self-ignition point 12
tray 12
Solvent bottles
connecting 15
Solvent selection valve 18
space requirements 14
standby 33
malfunction 33
switch on 32
T
Technical Data
gradient information binary 51
gradient information quaternary 51
technical data
general data 49
pump head 52
solvent conveyance 49
test
Installation Qualification 34
Operation Qualification 34
Torque 46, 47
transport damage 55
Troubleshooting
First Measures 35
troubleshooting
LAN 35
V
Valve block 21
Venting screw 7
W
warranty 55
P 6.1L user manual V6890
Latest manuals online:
www.knauer.net/downloads
www.knauer.net
HPLC · SMB · Osmometry
KNAUER
Wissenschaftliche Geräte GmbH
Hegauer Weg 38
14163 Berlin
Phone:
Fax:
e-Mail:
Internet:
+49 30 809727-0
+49 30 8015010
info@knauer.net
www.knauer.net
© KNAUER 2017