sympHony Dissolved Oxygen Probes

DOC022.53.80317
sympHony™ Dissolved Oxygen Probes
07/2012, Edition 1
User Manual
Table of contents
Safety information on page 3
Calibration on page 5
Specifications on page 3
Sample requirements on page 7
Product overview on page 3
Sample measurement on page 8
Meter compatibility on page 4
Maintenance on page 9
Preparation for use on page 4
Troubleshooting on page 11
Polarize the probe on page 4
Safety information
Precautionary labels
Read all labels and tags attached to the instrument. Personal injury or damage to the instrument
could occur if not observed. A symbol on the instrument is referenced in the manual with a
precautionary statement.
Electrical equipment marked with this symbol may not be disposed of in European public disposal
systems after 12 August of 2005. In conformity with European local and national regulations (EU
Directive 2002/96/EC), European electrical equipment users must now return old or end-of-life
equipment to the Producer for disposal at no charge to the user.
Note: For return for recycling, please contact the equipment producer or supplier for instructions on how to return endof-life equipment, producer-supplied electrical accessories, and all auxiliary items for proper disposal.
Specifications
Specifications are subject to change without notice.
Specification
89231-624
Measurement range 0.03 mg/L - saturation
Temperature range
89231-626
0.03 mg/L - saturation
0.3% to saturation
0.3% to saturation
0 to 50 °C (32 to 122 °F)
0 to 50 °C (32 to 122 °F)
Temperature sensor Yes
Yes
Electrolyte
Potassium-chloride based
Potassium-chloride based
Sensor
Platinum cathode and silver anode with a
replaceable membrane module
Platinum cathode and silver anode with a
replaceable membrane module
Body material
ABS
ABS
Length
120 mm
120 mm
Diameter
12 mm
12 mm
Cable
1m
1m
Connector
BNC and banana
MP51
1
For use with sympHony handheld meters only
Product overview
The dissolved oxygen probes measure the dissolved oxygen concentration in general aqueous
samples. Refer to Figure 1. Refer to Sample requirements on page 7.
The dissolved oxygen probes are used with sympHony meters. Refer to Meter compatibility
on page 4.
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Figure 1 Probe overview
1 Probe storage tube
3 Cathode
2 Anode
4 Membrane module
Meter compatibility
The dissolved oxygen probes with BNC and banana connectors are compatible with the sympHony
benchtop meter model(s): B40PCID.
The dissolved oxygen probes with MP5 connectors are compatible with the sympHony handheld
meter model(s): H10D and H30PCD.
Preparation for use
To prepare the probe for calibration or sample measurement:
1.
2.
3.
4.
5.
Turn the probe storage tube and remove it from the probe.
Rinse the storage tube with deionized water.
Invert and shake the storage tube to remove any water droplets.
Save the tube for storage.
After storage for more than 3 months, clean the probe before use. Refer to Clean the probe
on page 9.
6. Make sure that the membrane module is approximately 2/3 full with filling solution. Refer to Fill the
membrane module on page 10.
Polarize the probe
Before calibration or measurement of dissolved oxygen, polarize the probe.
Polarize the probe every time the probe is disconnected from the meter or the batteries are removed
from the meter. The probe is continuously polarized while it is connected to the meter.
1. Fill the probe storage tube with deionized water to the mark.
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2.
3.
4.
5.
6.
7.
8.
Put the probe in the storage tube and turn to tighten.
Connect the probe to the meter. Turn the meter on.
Wait for the probe to polarize. Refer to Table 1 for the amount of time.
Enter the atmospheric pressure and salinity on the meter.
Turn the probe storage tube and remove it from the probe.
Rinse the storage tube with deionized water.
Invert and shake the storage tube to remove any water droplets.
Table 1 Polarization times
Disconnect time
Polarization time
Less than 5 minutes
10 minutes
5–15 minutes
45 minutes
More than 15 minutes
6 hours
Calibration
Before calibration:
Prepare the probe for use. Refer to Preparation for use on page 4.
Prepare the meter. Refer to the meter manual.
Calibration notes
• For maximum accuracy, calibrate the probe at least once a day.
• For the best results, use the water-saturated air (100%) calibration procedure. Refer to Calibration
procedure on page 5.
• If a calibration error occurs, refer to Troubleshooting on page 11.
Calibration procedure
1. Turn the meter on. If
the probe is not
polarized, polarize the
probe.
2. Push Calibrate and
select the probe if
applicable.
3. Rinse the probe with
deionized water. Blot
dry with a lint-free cloth.
4. Add approximately
¼ inch (6.4 mm) of
deionized water to the
probe storage tube or a
narrow-neck bottle,
such as a BOD bottle.
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5. Invert and shake the
probe storage tube to
remove all water
droplets. If a BOD bottle
is used, put a stopper in
the bottle and shake the
bottle vigorously for
approximately
30 seconds to saturate
the entrapped air with
water. Then, allow up to
30 minutes for the
contents to equilibrate
to room temperature.
6. Immediately put the
probe in the storage
tube and turn to tighten.
If a BOD bottle is used,
remove the stopper and
put the probe in the
bottle.
7. Push Read. The
display shows "Stable"
when the reading is
stable. The calibration
is complete when the
reading is stable.
Calibration to a specific value
When TO A SPECIFIC VALUE is selected as the type of calibration, the probe is calibrated with a
single dissolved oxygen standard. Select the dissolved oxygen standard nearest to the expected
sample value.
1. Turn the meter on. If
the probe is not
polarized, polarize the
probe.
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2. Push Calibrate and
select the probe if
applicable.
3. Prepare the
dissolved oxygen
standard in a beaker or
an applicable container.
4. Rinse the probe with
deionized water. Blot
dry with a lint-free cloth.
5. Add approximately
¼ inch (6.4 mm) of the
dissolved oxygen
standard to the probe
storage tube or a
narrow-neck bottle,
such as a BOD bottle.
6. Invert and shake the
probe storage tube to
remove all water
droplets. If a BOD bottle
is used, put a stopper in
the bottle and shake the
bottle vigorously for
approximately
30 seconds to saturate
the entrapped air with
water. Then, allow up to
30 minutes for the
contents to equilibrate
to room temperature.
7. Immediately put the
probe in the storage
tube and turn to tighten.
If a BOD bottle is used,
remove the stopper and
put the probe in the
bottle.
8. Push Read. The
display shows "Stable"
when the reading is
stable. The calibration
is complete when the
reading is stable.
9. When the reading is
stable, use the arrow
keys to enter the
dissolved oxygen
standard value, then
push OK.
Sample requirements
Some probes are not compatible with specific sample types. Probe damage can occur.
• Samples should be aqueous. Measurements may be made in partially aqueous or some watermiscible solvents. The results must be interpreted with caution as the full pH scale is shifted when
the solvent system changes.
• Proteins can collect on the membrane. Make sure the probe stays clean when these types of
samples are measured.
• Do not use probes in solutions that are outside the temperature range of the probe.
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Sample measurement
WARNING
Chemical exposure hazard. Obey laboratory safety procedures and wear all of the personal protective
equipment appropriate to the chemicals that are handled. Refer to the current material safety data
sheets (MSDS) for safety protocols.
Before measurement:
Prepare the probe for use. Refer to Preparation for use on page 4.
Calibrate the probe. Refer to Calibration on page 5. The manufacturer recommends that the probe is calibrated
at least once a day for the best measurement accuracy.
Measurement notes
• For accurate measurements, stir all samples at a constant rate.
• When the probe is submerged, make sure that there are no air bubbles under the probe tip. Gently
shake the probe from side to side to remove any air bubbles.
• Do not put the probe on the bottom or sides of the container.
• If stabilization is slow, shake the probe from side to side in the solution.
• If a measurement error occurs, refer to Troubleshooting on page 11.
Measurement procedure
1. Turn the meter on. If
the probe is not
polarized, polarize the
probe and then
calibrate the probe.
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2. Rinse the probe with
deionized water. Blot
dry with a lint-free cloth.
3. Put the probe in the
sample and stir
vigorously with the
probe or a stir stand
and stir bar.
4. To measure bodies
of water, move the
probe up and down
approximately 3–
6 inches to make
motion around the
probe tip.
5. Push Read. Select
the channel for the
parameter to be
measured if applicable.
6. Stir vigorously. The
display shows "Stable"
when the reading is
stable.
7. If the folder icon is
shown, push the folder
icon to save the data.
8. Do steps 2–7 again
for additional
measurements.
Note: Data is
automatically saved if
the folder icon is not
shown.
9. When
measurements are
done, prepare the
probe for storage. Refer
to Storage
on page 10.
BOD measurement
The dissolved oxygen probes can be adapted for BOD measurement with the BOD Accessory Kit.
The funnel in the kit has a stir bar incorporated into the end. When the funnel is used with a stir
stand, the correct stirring motion necessary for BOD measurement is supplied. The spacer in the kit
positions the dissolved oxygen probe correctly in the funnel. The funnel allows for sample
displacement with insertion of the dissolved oxygen probe and limits exposure to atmospheric
oxygen.
Maintenance
Clean the probe
NOTICE
Use only deionized water and/or the polishing cloth to clean the anode and cathode. Refer to Figure 1 on page 4.
1. Remove the membrane module from the probe:
a. Hold the probe so it is vertical with the tip down.
b. Gently turn the membrane module to remove it from the probe.
2. Soak the membrane module in a mild soap solution.
3. Rinse the membrane module fully with deionized water.
4. Invert the membrane module and shake vigorously to remove any water.
5. Rinse the probe with deionized water. Blot dry with a lint-free cloth.
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6. Rub the anode with the polishing cloth supplied. The anode is the outer metallic stem of the probe
that is visible when the membrane module is removed. The polishing cloth removes deposits that
can decrease the probe performance.
7. Fill the membrane module 2/3 full with filling solution and assemble the probe. Refer to Fill the
membrane module on page 10.
Replace the membrane module
NOTICE
Carefully handle the membrane modules to prevent damage.
It is necessary to replace the membrane module periodically. Replace the membrane module when it
is damaged, the probe reading drifts or the probe has a slow response.
1. Remove the membrane module from the probe:
2.
3.
4.
5.
a. Hold the probe so it is vertical with the tip down.
b. Gently turn the membrane module to remove it from the probe.
Discard the used membrane module.
Rinse the probe with deionized water. Blot dry with a lint-free cloth.
Rub the anode with the polishing cloth supplied. The anode is the outer metallic stem of the probe
that is visible when the membrane module is removed. The polishing cloth removes deposits that
can decrease the probe performance.
Fill the new membrane module 2/3 full with filling solution and assemble the probe. Refer to Fill
the membrane module on page 10.
Fill the membrane module
Add filling solution to the membrane module if the level of filling solution in the membrane module is
less than 2/3 full.
1. Remove the membrane module from the probe:
a. Hold the probe so it is vertical with the tip down.
b. Gently turn the membrane module to remove it from the probe.
2. Add filling solution to the membrane module until it is approximately 2/3 full.
3. Install the membrane module on the probe:
a. Carefully tilt the membrane module to remove the air bubbles in the filling solution.
b. Hold the probe with the tip down and slightly tilted.
c. Slowly put the membrane module on the probe. Some filling solution should come out of the
threads of the membrane module. Remove any filling solution on the surface of the probe.
4. If no filling solution comes out of the threads, air may be inside the membrane module cap. Add
more filling solution to the membrane module and do step 4 again.
5. If the probe will be used immedately, polarize the probe. Refer to Polarize the probe on page 4.
6. If the probe will not be used immediately, put the probe in storage. Refer to Storage
on page 10.
Storage
Short-term (less than 2 hours) storage
Put the probe in a sample for a maximum of 2 hours if the sample pH is not too high and does not
contain corrosive agents.
Standard (less than 6 months) storage
1. Rinse the probe with deionized water. Blot dry with a lint-free cloth.
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2. Fill the probe storage tube with deionized water to the mark.
3. Put the probe in the storage tube and turn to tighten.
Long-term (more than 6 months) storage
Remove the filling solution from the membrane module for long-term storage.
1. Remove the membrane module from the probe:
2.
3.
4.
5.
a. Hold the probe so it is vertical with the tip down.
b. Gently turn the membrane module to remove it from the probe.
Rinse the anode and cathode with deionized water. Blot dry with a lint-free cloth.
Rinse the membrane module fully with deionized water to remove the filling solution.
Invert the membrane module and shake it vigorously to remove any water.
Install the membrane module on the probe:
a. Hold the probe so it is vertical with the tip down.
b. Gently install the membrane module on the tip.
6. Put the probe in the storage tube and turn to tighten.
Troubleshooting
For the best performance, make sure to:
• Prepare the probe for use before calibration or measurement.
• Obey the calibration and measurement notes.
• Obey the calibration and measurement procedures.
Troubleshooting notes:
• Magnetic stirrers may supply sufficient heat to change solution temperature. Put a piece of
insulating material between the stirrer and beaker.
• Protect the sensing element from direct light during measurement.
Refer to Table 2 for calibration warnings and errors. Refer to Table 3 for measurement warnings and
errors.
Table 2 Calibration warnings and errors
Error/Warning
Calibration out of range
Solution
The slope is out of range.
Calibrate the probe. Refer to Calibration on page 5.
Connect a new probe.
Standard or electrode in
poor condition
Calibrate the probe. Refer to Calibration on page 5.
Outside slope limits
Examine the dissolved oxygen standard solution. Make sure that the dissolved
oxygen standard used is the dissolved oxygen standard selected in the calibration
setup. Identify the temperature specification in the calibration setup. Use a new
dissolved oxygen standard.
Standard not recognized
Unstable reading Time t >
120 s
Examine the probe. Refer to Examine the probe on page 12.
Calibrate the probe. Refer to Calibration on page 5.
Examine the probe. Refer to Examine the probe on page 12.
Make sure that the probe is correctly immersed in the sample.
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Table 2 Calibration warnings and errors (continued)
Error/Warning
Electrode in poor condition
Solution
Examine the probe. Refer to Examine the probe on page 12.
Check the electrode
SAME BUFFERS
Calibrate the probe. Refer to Calibration on page 5.
Examine the probe. Refer to Examine the probe on page 12.
Examine the dissolved oxygen standard. Use a new dissolved oxygen standard.
Table 3 Measurement warnings and errors
Error/Warning
Measurement out of range
Solution
Examine the probe. Refer to Examine the probe on page 12.
("_ _ _ _ _ _")
Unstable reading
Examine the probe. Refer to Examine the probe on page 12.
Make sure that the probe is correctly immersed in the sample.
The temperature is out of °C
range.
Examine the temperature sensor.
Time > 120 s
Make sure that the probe is correctly immersed in the sample.
Time > 240 s
Measure the sample temperature.
Time > 300 s
Connect a different probe to identify if the problem is with the probe or the
meter.
Examine the probe. Refer to Examine the probe on page 12.
Examine the probe
Note: The lower the sample temperature or the larger the temperature difference between the samples, the longer
the stabilization time will be.
1. Clean the probe. Replace the membrane module if necessary.
2. Turn off the meter. Disconnect and then connect the probe again.
3. Connect a different probe to identify if the problem is with the probe or the meter.
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