For research use only
LE220-plus
USER MANUAL
Instrument and software developed for
high performance sample preparation
using adaptive focused acoustic energy
Product Name:
E/LE220-Series
Model Numbers:
LE220-plus
Software:
Document No. 010398
SonoLab 8
Rev A
Page 1
UNIVERSAL PRECAUTIONS
Universal Precautions should be followed on all specimen samples, regardless of whether a sample is known to contain
an infectious agent. Laboratories handling specimen samples are advised to comply with applicable parts of the following
governmental and clinical standards, or their equivalent in the country of use:
•
Centers for Disease Control (CDC), Universal Precautions for Prevention of Transmission of HIV and Other
Bloodborne Infections, published 1987, updated 1996
•
Clinical and Laboratory Standards Institute (CLSI), GP17-A2 Clinical Laboratory Safety; Approved Guideline Second Edition, published 2004, ISBN 1-56238-530-5
•
Clinical and Laboratory Standards Institute (CLSI), M29-A3 Protection of Laboratory Workers from
Occupationally Acquired Infections; Approved Guideline, Third Edition, published 2005, ISBN 1-56238-5674
•
Occupational Safety and Health Administration (OSHA), 29 CFR 1910.1030 Bloodborne Pathogens
•
International Standards Organization (ISO) 15190:2003, Medical Laboratories – Requirements for Safety
Trademarks
Patented or patent pending and registered or registration-pending trademarks of Covaris are protected.
Registered names and trademarks used in this document, even when not specifically marked as such, are not to be
considered unprotected by law.
WCS and AFA are either registered trademarks or trademarks of Covaris, Inc. in the United States and/or other
countries.
Clorox is a registered trademark of The Clorox Company.
Teflon is a registered trademark of The DuPont Company.
Information subject to change without notice
For Research Use Only
Not for use in diagnostic procedures
Publication P/N 010398
Revision B, October 2017
Product covered by Patent No. US6, 719,449, US6, 948,843, and other pending applications
Copyright © 2017 Covaris, all rights reserved.
Covaris, Inc.
14 Gill St, Unit H
Woburn, Massachusetts
01801-1721 USA
LE220-plus User Manual
Document No. 010398
Printed in U.S.A.
Rev B
Page 2
Warnings
For safety of operating personnel:
Make sure that the equipment is properly grounded. DO NOT operate if it is not properly grounded.
The unit is equipped with a power plug appropriate for the destination country. DO NOT, under any circumstances,
remove the grounding prong from the power cord.
Do not run an acoustic treatment with the Acoustic Assembly and sample cover in the UP position or without a water
bath - the acoustic system will not work.
If there is any indication that the Safety System is not functioning properly, DO NOT operate the equipment and
contact Covaris immediately.
If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment
may be impaired.
To prevent damage to the equipment:
The instruments are designed to operate in ambient laboratory conditions e.g., 19°C to 25°C (66°F to 77°C). DO
NOT operate the instrument in a cold room environment; the system is designed to operate with a water bath and
re-circulating heater/chiller apparatus to control sample temperature.
NEVER run a method without a water bath; this could damage the transducer. The instrument is equipped with a
water level sensor to protect the transducer and degassing pump. The system will not allow the degassing pump to
operate or the acoustic treatment to start unless an adequate volume of water is detected.
Distilled or deionized water should be used to fill the water bath.
Unless a Water Conditioning System 2.0 (WCS) is employed, empty the water bath and wipe it dry EVERY day with
a lint-free cloth. DO NOT leave water in the tank for an extended time as there is no water filtration or water
cleaning system within the apparatus (unless WCS is employed).
Do not employ isopropyl alcohol, ammonia-based or abrasive cleaners on the water tank, as these will damage the
acrylic surfaces.
Establish a standard of operation and periodically test equipment, as described in Maintenance (see section 6.0) of
this manual.
Loading third party software onto the computer may interfere with system operation. Please consult with Covaris.
LE220-plus User Manual
Document No. 010398
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Warranty
When used in accordance with written instruction and under normal operating conditions, the Covaris instruments
are guaranteed to be free of defects in MATERIAL and WORKMANSHIP for one (1) year from the date of original
delivery by an authorized representative. Any component which proves defective during the stated period will be
repaired free of charge or replaced at the sole discretion of Covaris, F.O.B., Woburn, Massachusetts, U.S.A.
provided the defective component is returned properly packaged with all transportation charges prepaid. The
customer is expected to perform basic diagnostics and component replacement with telephone support from Covaris
personnel. If Covaris personnel are required to perform on-site repair, all travel related costs are paid by the
customer. A limited warranty as specified may apply to certain components of the equipment.
Warranty Exceptions
This warranty is void if failure of the software or hardware has resulted from accidents, abuse, improper
maintenance, or repair, or misapplication by the customer. It is also void if damage is caused by any unauthorized
attachments or if modifications are made to the equipment. Removing or tampering with the Safety Enclosure will
void the warranty, and the customer will assume all liabilities.
This warranty is limited to the original purchaser and is not transferable.
The software will perform according to the accompanying written materials and the medium on which the software is
delivered is free of defects in materials under normal use and service. The warranty is void if damage has resulted
from third party software not intended for use with the system.
The high power focused transducer is designed to give maximal mechanical energy output in water. Permanent
damage to the transducer and electronic circuits could result if the transducer is operated without water. Operation
of the system without water in the water bath voids the warranty.
CONTACT COVARIS, INC. SHOULD YOU HAVE ANY QUESTIONS CONCERNING EQUIPMENT
Warranty Services
The purchased equipment is covered by a twelve (12) month warranty which includes all the service and support
necessary so that the customer can operate the equipment successfully. Extended warranties are available at the
end of the original 12 month warranty period.
Services included with the original purchase of the system are:
Technical Support – On-going assistance with the operation or application of the equipment and/or troubleshooting
is provided via:
•
•
Telephone
o
United States: Tel: +1 781 932 3959 during the hours of 9:00am to 5:00pm,
Monday through Friday, Eastern Standard Time (EST), Greenwich Mean Time
(GMT-05:00)
o
Europe: Tel: 44 (0) 845 872 0100, during the hours of 9:00am to 5:00pm,
Monday through Friday, Greenwich Mean Time
E-mail queries to techsupport@covaris.com or applicationsupport@covaris.com
Parts Replacement – Replacement of parts (excluding consumables) from normal operation of equipment are
provided on a priority basis. All labor and shipping charges are included. Failure due to accident, abuse, or
improper operation is not covered.
An Operator’s Manual is provided with the equipment. This manual includes sections on the operating
instructions, maintenance guidelines, and troubleshooting tips.
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Contents
1.0
INTRODUCTION ................................................................................................................................ 6
2.0
INSTALLATION AND SETUP ........................................................................................................... 7
2.1
Assembly ...................................................................................................................................... 7
2.2
Installation .................................................................................................................................... 8
3.0
GETTING STARTED ....................................................................................................................... 12
3.2
Start the System ......................................................................................................................... 12
3.3
Preparing the LE220-plus for Use ............................................................................................ 14
3.3.1 Instrument Status & Basic Controls ............................................................................................ 14
3.4
Running a Method ...................................................................................................................... 22
3.5
Daily System Shutdown ............................................................................................................ 23
4.0
CREATE AND EDIT METHODS ..................................................................................................... 23
5.0
SonoLab™ APPLICATION SOFTWARE ....................................................................................... 29
5.1
Home Screen .............................................................................................................................. 30
5.2
History Tab.................................................................................................................................. 30
5.3
About Tab.................................................................................................................................... 32
5.4
ADMIN Tab .................................................................................................................................. 33
6.0
SYSTEM SPECIFICATIONS ........................................................................................................... 37
7.0
MAINTENANCE ............................................................................................................................... 38
8.0
TROUBLESHOOTING..................................................................................................................... 41
Appendix A: LE220-plus Series Site or Transport Preparation ........................................................... 43
Appendix B: Installing SonoLab Software, Firmware and Instrument Drivers ................................... 45
Appendix C: Humidity and Water Management in the Covaris LE220-plus ......................................... 50
Appendix D: LE220R-plus......................................................................................................................... 51
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1.0
INTRODUCTION
1.1
Overview of the User Manual
This manual contains operation and maintenance instructions for the Covaris LE220-plus Focusedultrasonicators. This document contains information essential to the proper use and care of the
equipment.
Should any unforeseen problems occur with the normal operation of the equipment, contact Covaris
Technical Support immediately.
The following definitions apply in this manual:
NOTE:
Inconvenience if disregarded.
CAUTION:
Equipment damage may occur.
WARNING:
Personal injury may occur.
There is also a PDF version of this manual, typically available on the computer desktop. Open the
manual by double clicking the user manual icon.
1.2
The LE220-plus Instrument and the Covaris Process
The LE220-plus Focused-ultrasonicators are based on Covaris Adaptive Focused Acoustics (AFA)
technology. These instruments are designed to provide researchers an integrated stand-alone tool to
be used in a laboratory environment. The instrument is ideal for those who work with tissue
homogenization, cell lysis, DNA or chromatin shearing, and other sample preparation steps.
The system is comprised of the Treatment System which delivers the acoustic energy to the sample,
the Safety System which protects users from inadvertently contacting the high intensity acoustic
energy, the Computer and Application Software which provides users an interface to control the
device, and the Water Management System consisting of a cooler/heater and a UV treatment
chamber with particulate filter to maintain water quality in the instrument bath.
The Covaris Process uses adaptive focused acoustic energy to precisely control cavitation and
acoustic streaming within the sample treatment vessel in a non-contact, isothermal fashion. Focused
acoustic energy is generated and delivered to individual samples. The mechanical energy imparted
on the sample results in a controlled series of compression and rarefaction events.
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2.0
INSTALLATION AND SETUP
2.1
Assembly
The following items should be found in the crate containing the instrument:
•
•
•
•
•
Main Unit
User Manual
Notebook Computer with AC Adapter
Cables
o USB cable
o Instrument power cord
o Computer AC Adaptor power cord
Accessories
o AFA ultraCUBE, consisting of:
 Water Conditioning System 2.0
 CH05 Chiller
o Chiller Hose Kit
o Sample Tubes & Racks (varies by application)
o Water Tank (installed in main unit)
E/LE Series Focused-ultrasonicator
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2.2
Installation
2.2.1
Unpack the instrument, the Water Conditioning System 2.0 (WCS), and the CH05
chiller.
Refer to the WCS User Manual to install the quartz tube and UV lamp into the WCS.
The quartz tube and lamp are shipped separately to prevent breakage during shipment.
Arrange the instrument, WCS, and chiller on a bench as shown below. The chiller and
WCS may be installed on either side of the LE220-plus. The CH05 and WCS may also
be placed on the floor, if they can be located such that the hoses reach the front of the
LE220-plus water bath.
CAUTION: When positioning the system, please keep the fan and rear panel vents
free of obstructions that may block the flow of air.
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Connect the hoses between the WCS and the CH05 as shown above.
Connect the remaining two hoses between the two ports on the LE220-plus water bath
and the remaining two ports on the opposite side of the WCS. It doesn’t matter which
bath port connects to which WCS port for these two hoses.
The WCS contains a particulate filter and UV lamp to prevent biological growth. This
allows the water bath to be changed just once per month. Refer to the User
Maintenance section of this manual for details.
2.2.2
Plug the instrument into a power source using the enclosed line cord. The instrument is
a “universal input” device and automatically employs any worldwide line voltage or
frequency (100 to 240 volts AC, 50 or 60 Hz). Leave the instrument power and
notebook power switched OFF at this time.
2.2.3
Plug one end of the USB cable into the instrument USB Port, and the other end into any
USB port on the notebook computer.
2.2.4
Connect the temperature control cable between the D-connector at the rear of the
LE220-plus and the D-connector on the CH05 chiller.
2.2.5
Connect the CH05 to a power source. The CH05 is a universal input device. Do not
turn on the CH05 at this time.
2.2.6
Locate the WCS power supply and line cord. Connect the WCS power supply to the
port provided on the WCS and connect the line cord to a power source. The power
supply is a universal input device. Leave the WCS turned off at this time.
2.2.7
Connect the computer to a power source. Connect the mouse or other accessories to
the computer.
2.2.8
Refer to the rear of the instrument’s right side panel and identify the following features
as necessary:
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USB Port
Product Safety Label
AC Line (Mains) Input
and Power Switch
Serial Tag,
Regulatory and
Line Power Label
The Rear Right Panel of the System
2.2.9
Refer to the figure below and identify the following items:
Enclosure Door
Safety Lock
Enclosure Door
Handle
Door Lock Release
Button
Stop Button
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2.2.10 Power on the instrument using the power switch at the right rear, at the power entry
module.
2.2.11 Press and hold the green ‘Door’ button while pulling the door handle to open the safety
enclosure front door. Slide out the water tank and remove it from its plastic shipping
bag. Remove and discard the desiccant pack.
Safety Enclosure
Water Tank
Safety Enclosure and Water Tank
2.2.12 The instrument is now ready to be started.
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3.0
GETTING STARTED
3.1
Fill the Water Bath
The instrument’s transducer must be immersed in a water bath to transmit
high-frequency, focused acoustic energy to the sample tube. For best
performance, employ only clean, distilled or deionized water in the bath.
Open the front door, remove the tank and the first time the LE220-plus is
operated, initially fill the bath about half full. The first time the WCS and
chiller operate, a substantial amount of air is forced from the WCS into the
bath, which may cause a full bath to overflow. This only occurs the first
time an empty WCS is used.
Once the WCS is filled with water, the bath should be filled to a level of
15.. If the transducer is in the raised position, out of the bath, fill to 15 on
the “Fill” side of the gauge. If the transducer is submerged, fill to 15 on the “Run” side of the gauge.
3.2
Start the System
Turn the instrument power ON using the power switch located on the rear, right
side of the instrument at the power entry module. When power is ON, the green
door-lock release switch will illuminate and the ventilation fans in the rear panels
will run.
Power on the WCS and CH05 chiller. SonoLab software will perform all temperature control; it is not
necessary to adjust temperature set points from the CH05 control panel.
The first time the chiller and WCS are operated, the WCS filter and UV treatment chamber are
empty, and this air is purged as described above. This is normal, and only occurs the first time the
chiller/WCS assembly are operated.
Start the system computer. The computer employs a Windows® 10 (or later) Operating System.
SonoLab is an application used to control a Covaris Focused-ultrasonicator for processing a
biological or chemical sample. It is designed to create, edit, and run a processing program called a
method. It has provisions to run, pause or stop a method and view execution histories which contain
a record of key instrument parameter values during a run. It also contains editors for customizing
system settings and performing maintenance functions.
SonoLab is installed on the computer purchased with the instrument. No further installation or
configuration is required.
Find the SonoLab icon on the desktop:
If the icon is not visible, or if SonoLab is not installed, please refer to Appendix C or contact Covaris
for installation instructions.
Double-click on the SonoLab icon to start SonoLab; the splash screen will appear:
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Following the splash screen, the SonoLab Home Screen will appear with the login dialog box in front
of it..
SonoLab is preconfigured with a single administrative user named “Administrator” whose password
is “admin”. User Authentication and creating new users and their permissions are described in
Section 5.4, the ADMIN Tab.
Enter the user name and password and click ‘LOGIN’
Following login, the motion system homing dialog appears:
Click ‘Yes’. The transducer and sample holder mechanical assemblies will move into position and
the SonoLab ‘HOME’ screen will display.
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If SonoLab does not connect to the instrument, the following screen appears allowing SonoLab to be
run in emulation mode, without an instrument.
Click “Yes”, and the normal SonoLab login and Homing screens are presented, allowing the user to
operate SonoLab as if an instrument is present. This is useful for training purposes or for creating
methods off-line.
3.3
Preparing the LE220-plus for Use
3.3.1
Instrument Status & Basic Controls
To run an acoustic treatment, a number of conditions must be met, including:
a)
b)
c)
d)
e)
f)
g)
The motion system must be initialized.
There must be an acoustic method selected in the Method box.
Water temperature must be within acceptable Min and Max limits.
The bath water must be degassed.
Water should be approximately at level 15.
The proper plate and consumables must be in place on the LE220 sample tray.
The safety enclosure door must be closed.
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The Run button will remain gray and inactive until a method is selected and all necessary
conditions are met. The LE220-plus automatically sets the temperature when a method
is selected.
To achieve an acceptable status on the other conditions:
a) The LE220-plus motion system normally initializes when SonoLab starts, unless
the user purposely skipped this step. In this case, the motion system can be
homed from the Motion Control pane.
Click on the Motion Control button to display the Motion Control pane.
Click on “Home” to initialize the motion system. See additional information for the Motion
Control pane in this section of the manual under ‘Other Controls on the HOME Screen’.
b) To select and load an acoustic method, click on the panel displaying the currently
loaded method, as shown below, and a list of existing method names will be
displayed in a drop-down list. Navigate through the list with the vertical scroll bar
and select a method.
Currently loaded method
Drop-down list of available methods
The method’s run time (minutes and seconds) and temperature set point will be
displayed in the lower right corner of the main screen as shown.
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c) An important principal of the Covaris Focused-ultrasonicator is isothermal
temperature control. The LE220-plus automatically adjusts the water
temperature, as necessary, to match the method temperature set point once a
method is selected. Water Temperature status will turn green when temperature
is within the Min and Max limits (by default 5 degrees above or below the set
point). The default set point (in the absence of a selected method) is 20ºC.
Bath cool down from room temperature to below 10 degrees takes about 30
minutes. Once a method starts, if the bath temperature rises beyond the user
specified limit, the LE220-plus finishes the current row being treated, pauses
treatment, and automatically resumes when the temperature is back within limits,
with no operator intervention required.
d) Properly degassed water is important for efficient transfer of acoustic energy from the
instrument’s transducer to the sample being treated. If water is detected in the bath, the
degassing pump is automatically turned on when SonoLab is started.
The pump should be allowed to run until the Degas indicator on the Status Screen turns
green (60 minutes) prior to an acoustic treatment.
The degas pump can be manually turned on and off from the Water Works pane.
See additional information below in section Other Controls on the HOME Screen.
e) When the “Run” button is clicked to start the method, SonoLab checks that the
bath water is at an appropriate level for the consumables employed in the
method. If the water level is incorrect, the method is paused and a message to
adjust the water level is presented.
The remaining conditions, along with their related status, are displayed in the Instrument
Status panel. Click the “System Status” button to display the Instrument Status panel.
An acceptable condition is indicated by a green check mark ( ) in the Status box. If a
condition is not acceptable, the status will show (X).
If any of the items other than “Water Level” and “Temperature within Limits” in the Status
list display (X), a red X will also be displayed in the System Status button, as shown
below. Water level and temperature will be adjusted as necessary once a specific
method is started.
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A red X in any items in the Status list…
… results in a red X in
the System Status button
A checkbox is provided at the bottom of the status screen to override the degas timer and
temperature limits to allow the LE220-plus to run a method without waiting for these
conditions to be met. With the temperature and degas override boxes checked, the
display changes to yellow caution indicators as shown.
Once a method has been loaded and all conditions are acceptable, the ‘Run’ button will
turn green and the selected acoustic Method can be started.
To modify an existing method, see Section 5 Create and Edit Methods.
3.3.2
•
Other Controls on the HOME Screen
Lightbulb: This is both a control and indicator. The
enclosure light is off when SonoLab starts. Clicking on
the light bulb image will turn the light ON, clicking again
will turn it OFF.
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•
Water Works Button
Water Works allows the user to control the chiller and degas pump either manually or by
automatic scheduling. For example, the schedule can be set to begin the degassing and
chilling early in the morning so that the LE220-plus will be ready to use when personnel
arrive.
Click on the “Water Works” button at the lower right of the Home screen to display the Water
Works pane.
The Water Works pane contains the following:
o
ON/OFF switches for chiller and degas pump: these are both switches and
indicators. The current status is highlighted in blue, as shown above. These
switches are disabled when operating in Auto (scheduled) Mode.
o
Current chiller set point, current LE220-plus bath temperature, and current LE220plus bath water level.
o
Enable/disable switch for automatic temperature and degas pump control: this is
both a switch and indicator. It toggles between Auto Mode , as shown above, and
“Click for Auto” when automatic control is not active. In Auto Mode, the chiller,
chiller temperature set point, and degas pump are controlled by the user defined
schedule.
o
Enable/disable Standby Mode button: this is both a switch and indicator. It toggles
between “Click for Standby Mode” and Standby Mode . In Standby Mode, the
chiller cooler/heater element is disabled and the LE220-plus transducer is raised out
of the water bath. Scheduled temperature control and degassing are disabled when
the instrument is in Standby Mode.
Automatic Chiller/Degas Operation
An automatic schedule for degassing and chilling the LE220-plus water bath is a convenient
way to have the instrument ready to use at the beginning of the day. Under software control,
the LE220-plus water bath will automatically activate degassing and adjust to the user
specified temperature at the beginning of the schedule period and turn off the degas pump
and place the chiller in standby mode at the end of the scheduled period. In standby mode,
the chiller’s heating/cooling circuit is disabled while the pump continues to operate,
circulating bath water through the WCS filter. See the ultraCUBE User Manual for details of
chiller and WCS operation.
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If a treatment method requires a different temperature from that set by the schedule, the
temperature specified in the method will override the scheduled temperature. Once the
method is finished, the bath temperature will remain at this new temperature until another
method changes it, or the next scheduled temperature change occurs.
To configure a schedule, perform the following steps:
o
Double click anywhere in table shown in the image above to display the
Automations Settings window. Note: to configure a schedule for just one specific
day, Sunday through Monday, double-click on that day in the table.
o
Click in the check boxes for the functions to be scheduled. Normally, all four boxes
will be checked.
o
Click on the clock icon for Degas on, circled above. This displays a clock.
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o
Click on either AM or PM, then on the hour the degas pump is to start. The clock
changes to display the hour selected (7 o’clock in this example) and the minutes
part of the time highlighted.
o
Click on the number of minutes after the hour. The clock display closes and the
user is returned to the Automation Settings screen.
o
Repeat the above steps for Degas off, Chiller on, and Chiller off.
o
Note: the scheduler forces the chiller start time to be at least 30 minutes after the
degas start time. Cold bath water does not degas efficiently. The 30 minute delay
permits the water to be sufficiently degassed before it is too cold. Once degassed,
the system will maintain a sufficiently degassed condition regardless of water
temperature.
o
Enter the desired chiller set point, in whole degrees, in the Default Temp ºC field.
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o
Click on either All, Weekdays, of the single day, in this example Sunday, button at
the bottom of the Automation Settings screen. The Automation Settings screen
closes and the Water Works pane is displayed.
In this example, the Weekdays button was clicked.
o
Click Auto Is Off. The button changes to a red Auto Mode button, and chilling and
degas will start and stop as scheduled on weekdays.
In Auto Mode, the chiller and degas buttons at the top of the Water Works pane are
disabled.
Manual Chiller and Degas Control
To manually control degassing and chiller operation, click the red Auto Mode button. The
button will change to Auto Is Off, indicating that manual control is enabled.
In Manual Mode, the chiller and degas pump may be toggled on and off by clicking the
buttons at the top of the window.
Clicking the “Click for Standby Mode” button places the LE220-plus and ultraCUBE in
standby. The transducer and sample tray move to the service position, the degas pump
turns off, and the chiller heating/cooling unit turns off. The chiller pump will continue to
operate, circulating the bath water through the WCS unit.
Note: If the LE220-plus is left in Standby mode, ie, the transducer is up and out of the
water bath, automatically scheduled events will not occur.
•
Motion Control Button
Clicking the Motion Control Button displays the Motion Control Pane:
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3.4
o
Home: Moves all motion axes to their initialized position, as occurs when the
software starts.
o
Load Position: Moves the tray to the front of the safety enclosure to facilitate
loading samples.
o
Start Position: Takes the sample tray to the treatment position of the first row of the
currently selected method. This position may be used to verify that the water level
in the bath is correct.
o
Service Position: Raises the sample tray and the transducer assembly allowing the
water bath to be removed from the instrument.
o
Stop: Stops any motion currently in progress.
Running a Method
When the “Run” button is green, the selected method may be started.
Click the “Run” button to start. The instrument will verify that water level
is appropriate for the specified for the method, and begin the treatment.
Once a method starts, if the bath temperature rises beyond the user specified limit,
the LE220-plus finishes the acoustic treatment of the current row of the plate, then
pauses, and automatically resumes
treating the remaining rows when the
temperature is within limits, with no
operator intervention required.
As the method runs, the graphic for the
row under treatment flashes green, and
the actual average incident power and
temperature data are displayed, as
shown.
The user may, at any time while running a method, click “Pause” to
interrupt a running method.
When the method is paused, the user may continue
the method by clicking the Resume button, or
terminate the method by clicking the Abort button.
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When the method has finished, the message “Method Complete” is displayed. Click
“OK”, then press the green door lock button and open the safety enclosure door.
Remove the treated sample plate.
For methods with high wattage (> 125 watts) and low bath temperature, it is
recommended to allow 5 to 10 minutes between plates to allow bath to cool.
3.5
Daily System Shutdown
If the instrument will be idle for an extended period of time (e.g. longer than two weeks), refer to the
system shutdown procedure in the User Maintenance section of this manual.
When the LE220-plus is finished being used for the day, leave the transducer submerged in the bath
if automatic scheduling is active. At the end of the scheduled period, the scheduler will turn off the
degas pump and disable cooling in the chiller, then automatically restart both as scheduled for the
next day. SonoLab software must be left running and the LE220-plus and ultraCUBE powered on
for the scheduled events to occur.
If automatic scheduling is not in use, click on the ‘Water Works’ button and then the ‘Click for
Standby’ button. The transducer will rise out of the bath and the ultraCUBE chiller’s heater/cooler
will turn off, but the chiller’s pump will continue to operate, circulating bath water through the
ultraCUBE water conditioning system. Note: no scheduled events will occur when the instrument is
in “Standby” mode, regardless of whether or not SonoLab is in “Automatic” (scheduled) mode.
CAUTION: Do not turn off either the WCS or CH05 chiller. These must remain
powered on to treat the bath water against bio-growth.
Leaving water in the water bath for an extended time without the WCS operating may
promote algal growth in the tank and lines, This growth can interfere with water quality
and sample processing.
4.0
CREATE AND EDIT METHODS
The SonoLab Method Editor supports the creation of new methods and editing of existing
ones.
A method consists of one or more Processes. A Process contains at least one
Treatment. Most methods will contain a single process, containing a single treatment
step.
If more complex methods are required, they are easily constructed
4.1
To create a new method, click the “New” button on the main screen (see Figure 5.1).
Figure 4.1 – New Method Button on Main Screen
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The Method Editor window will appear (See Figure 5.2).
Method Name
Plate Definition
Drop-down List
Add Process
Figure 4.2 - Method Editor Window
1) Enter the name of the new method in “Name” box as shown.
2) Select the appropriate plate for the consumables used in this method
from the “Plate Definition” drop-down list.
4.1.1
Create or Add a Step in the Method
Click the
New button.
The process bar is displayed.
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Click the
4.1.2
button inside the process bar to display the step type menu.
Choosing Treatment allows editing of the four acoustic parameters: Duration,
Peak Incident Power, Duty Factor, and Cycles per Burst.
Figure 4.3 - Add a Treatment
Treatment parameters are chosen by directly editing the Value box. The treatment
parameters are:
Peak Incident Power (PIP)
This parameter measures the peak electrical power used to drive the ultrasound
transducer. In the LE220-plus, the adjustable range of Peak Incident Power is 0.1 to
500 Watts.
Duty Factor
This parameter defines the ratio between the on-time of the transducer and total
operation time, and is expressed as a percentage. For a given Peak Incident Power,
a larger Duty Factor results in more acoustic energy generated from the transducer.
Users can define the Duty Factor from 0.1% to 50% in the LE220-plus.
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The product of the Peak Power and Duty Factor gives the approximate Average
Incident Power (AIP) generated from transducer. For example, if Peak Incident
Power is set to 10 Watts and Duty Factor is set to 15%, the Average Incident Power
will be approximately 1.5 Watts. However, the real average incident power from the
transducer may vary due to factors such as sample tube type, water level and water
temperature.
Cycles per Burst
This parameter indicates the number of electrical signals used in each acoustic
“burst” of the transducer.
One may define Cycles per Burst (cycles/burst) from 50 to 1000 in the LE220-plus for
different applications. Advanced users may use this parameter to fine tune their
experiments.
Duration
This parameter defines the amount of time for which the sample is under a treatment.
The measurement of the Duration is in seconds. The maximum time for any
treatment is 3600 seconds. Should a longer treatment be necessary, “Duplicate
Steps” can be used to extend the treatment.
SonoLab displays the Average Incident Power, calculated from the Peak Incident
Power and Duty Factor, adjacent to the treatment parameters in the treatment step
bar. In the LE220-plus, the maximum Average Incident Power is 250 Watts (500
watts Peak Incident Power and 50% Duty Factor).
Dithering
The LE220 motion system provides dithering motion capability in the X axis (fore-&aft), Y axis (left-to-right), and Z axis (up-and-down). If a treatment specifies dithering,
click on the “Pen-&-Paper” icon on the right end of the Process bar to display the
Dithering Parameters pane.
Figure 4.4 – Dither Parameter Pane
Enter the specified dithering parameters and click “OK”.
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Temperature Set Point and Range
Enter the temperature set point for the protocol and the allowable range into the text
entry fields. SonoLab allows a minimum range of plus/minus 3 degrees.
Enter Temperature
Set Point and Range
.
Figure 4.5 – Entering Temperature Set Point and Range from lower to upper
limits
4.1.3
Once the treatment parameters have been defined, click in the process bar
to highlight it, then click in the treatment well graphic to define which plate
positions will be treated. The LE220-plus treats an entire row at a time.
Therefore, clicking anywhere in a row causes the row to be selected. Any
number of rows may be selected for treatment; rows selected for treatment
turn from white to yellow. Clicking on a row a second time removes it from
the list to be treated. When the method is run, the graphic for the position
being treated flashes green, indicating which position is under treatment.
Figure 4.6 - Rows 1, 3, 6, & 9 Selected for Treatment
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4.1.4
Choosing a Delay step introduces a fixed time delay into a method, allowing
the sample to settle between two different treatment steps, for example. The
delay is specified in seconds. See Figure 4.7.
Enter Delay in Seconds
Figure 4.7 - Add a Delay Step
4.1.5
Choosing a Prompt step displays a specified message on the screen and
pauses execution of the method until the operator clicks OK.
Define the prompt message by clicking on the “pen & paper” button of the
prompt bar to display the text entry field for the prompt. Enter the text of the
prompt, and click OK. See Figure 4.8.
Click to create or edit
text of the prompt.
Figure 4.8 Add a Prompt Step
When the prompt step is reached during the treatment, the method pauses,
and the prompt message will be displayed as shown in Figure 4.9.
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Figure 4.9 – Example of a Prompt Message Display
4.2
4.1.6
The “Creation Notes” section is a text entry box at the right side of the
Method Editor screen in which the user may enter, for example, descriptive
text relating to the method being created. This field may be left blank if the
user so chooses.
4.1.7
When all method parameters and steps have been defined, click on
to save changes.
To modify an existing method, select the method as described above, then click the
“Edit” button. The method editor window will be displayed. Make desired changes
and click ‘Save’. The user is then prompted to confirm that they wish to overwrite the
existing method.
… then Click “Edit”
Select a Method to Edit
Here…
Figure 4.10 – Selecting a Method to Edit
5.0
SonoLab™ APPLICATION SOFTWARE
The main components of SonoLab software include:
HOME – compose or select methods to run
HISTORY – view system usage history
ADMIN – Add and configure SonoLab user accounts (only available to admin users)
ABOUT – information on system configuration
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5.1
Home Screen
The Home Screen is the principal user screen for the system. See Section 3.3 for details
on operating the instrument through the Home Screen.
5.2
History Tab
The History tab (Figure 6.2) provides a scrolling list containing the history of every
method performed on the instrument. This list can be sorted by method name, date the
method was run, the operator who ran the method, or the run status (complete, aborted,
failed).
Figure 5.1 – The History Tab
Double-click on a line to display its details. The left side of the screen will display textual
information concerning the method. A pane on the right side contains graphs of the
temperature and power levels generated for each sample treated by the method. This
pane scrolls as necessary to display the graphs of all samples treated by the method.
See Figure 5.2.
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Figure 5.2 – Details of a Method History
Double-click on one of the graphics on the right side. The graphic will be outlined
in orange, indicating it has been selected, and additional information pertaining to
the part of the method which generated the specific graphic is displayed at the
lower left. See Figure 5.3.
Figure 5.3 - Additional History Details
Note: Occasionally, a history file may be corrupted. If this occurs, clicking on the
“History” tab will result in an error message similar Figure 5.4:
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Figure 5.4 – Message Indicating a Problematic History File
If this occurs, click the “OK” button to continue to the “History” screen. However, the
error will be presented each time the “History” tab is selected, until the offending file is
removed.
To remove the bad file, navigate to Documents\Covaris\SonoLab\History, then locate the
offending file, in this example, “fddf_04_14_2016-09-10-38.xhst”. Drag this file to the
Recycle Bin.
5.3
About Tab
The About Tab provides user basic information about the instrument such as serial
number, software version, board serial numbers etc.
Figure 5.5 - About Tab
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5.4
ADMIN Tab
User Authentication
The User Authentication feature allows only authorized operators to log onto the LE220-plus
system. User Authentication defines two levels of operators, Administrators and Users.
Permission Levels
An Administrator:
•
Can add or edit other Administrator level users.
•
Can add or edit User level users.
•
Can assign access to specific methods to Administrators and Users.
•
Can run and edit any methods installed on the system.
•
Can run and edit methods assigned to them by an administrator.
•
Can create methods.
•
Can edit and run methods the user created.
A User:
Additionally, a User can review the history screens and system configuration information.
Managing Users
Figure 5.6 – Admin Tab
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5.4.1
Click to select a user. Then click the ‘View’ button to display a user’s details.
A dialog box with the user’s ‘User Name’, first name, last name, and ‘Role’
(Administrator or User) is displayed above a scrolling list of methods assigned to the
user.
Figure 5.7 – View User Screen
5.4.2
Clicking on ‘Edit’ produces ‘Edit User’ window.
Figure 5.8– Edit User Screen
From this window, the user’s name and role may be edited and methods may be added
or removed from the methods available for the user to use. When a method in the list
on the right is selected, the button between the available methods becomes a left
pointing arrow, indicating the selected method will be copied to the ‘Assigned Methods’
list. If a method is selected in the ‘Assigned Methods’ list, the arrow points to the right,
indicating the method will be deleted from the user’s method list.
A user can only run methods in their “Assigned Methods” list, or methods they create.
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5.4.3
Click the ‘Save’ button to save changes, the ‘Cancel’ button to exit without
changes.
5.4.4
Selecting a user and clicking the ‘Delete’ button presents a confirmation
dialog box. Click ‘Yes’ to confirm that you are deleting the user; click ‘No’ to
exit without deleting the user. Once a user is deleted, the user cannot be
reactivated, nor can that user name be reused.
Figure 5.9 – Deleting a User
5.4.5
Clicking the ‘Add’ button displays the ‘Add/Edit User’ window.
Figure 5.10 – Add User Screen
•
Select the Role from the drop-down list.
Figure 5.11 – Select User’s Role
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•
Fill in the User, First, and Last Name fields, and select methods to be
assigned to the new user.
Figure 5.12 – User ID and Method Selection
•
Click the left-pointing arrow to add the methods to the user’s assigned
method list.
Figure 5.13 – Methods Assigned to User
•
Click ‘Save’ to add the user to the data base.
When a new user is created, the user will be prompted to create a password the first time the
user tries to log in.
The user enters their new password, and then must enter it again to verify correct spelling.
Clicking “OK” returns the user to the login screen, where the user may now enter the new
password and log in.
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6.0
SYSTEM SPECIFICATIONS
Treatment Power:
Dimensions:
Weight:
Bench-top; high intensity acoustic transducer, temperature monitoring
device, circulation pump, water bath with safety enclosure
500 Watts Peak Incident Power, 250 Watts Average Power
23” W x 30” D x 19” H (59cm x 76cm x 48cm)
approximately 110 lbs. (50 kg)
Power Requirements:
100-240 VAC 500 VA maximum, 50-60Hz
Ambient Temp. Range:
19°C to 25°C (66°F to 77°C)
Ambient Humidity Range:
30% to 70%
Regulatory Labeling:
Water Bath:
Temperature Alarms:
Computer:
CE, ETL Mark (for Product Safety), WEEE
Complies with Low Voltage Directive 2006/95/EC. Certified to
IEC/EN/ANSI/UL 61010-1:2004 and CAN/CSA C22.2 No. 61010-1:2004,
2nd Edition “Safety Requirements for Electrical Equipment for Measurement,
Control and Laboratory Use, Part 1: General Requirements”
Complies with Class A Industrial/Scientific/Medical (ISM) equipment under
EN 61326-1:2005, EN 61000-3-2:2004 and EN 61000-3-3:1995 for EU EMC
Directive 2004/108/EC. Also FCC Part 15 Class A radio emissions
requirements for the USA and ICES-003 Class A for Industry Canada.
Distilled or deionized water only
Can be programmed from +0.5°C to +55.0°C
Notebook computer typical
Operating System:
Microsoft Windows 10 or later
Application Software:
Covaris SonoLab
Data Input:
Keyboard, mouse
Treatment System:
Safety:
EMC:
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7.0
MAINTENANCE
7.1
Cooling Air Intake
Periodically, unplug the unit and remove any obstruction to the fans and air vents on the rear panel
to ensure that objects are not restricting the flow of air.
7.2
Water Bath
7.2.1
Recommendations for Daily Maintenance
Only distilled or deionized water should be used to fill the water bath. To avoid algae growth, the
water bath should be emptied, the degas lines purged, and the tank dried prior to longer term
(more than a week) idle time. See ‘Shutdown for Long Term Storage below.
7.2.2
Recommendations for Monthly Maintenance
Periodically, the water bath and degassing lines should be rinsed with a 10% solution of Clorox
bleach. The resultant sodium hypochlorite solution is ~ 0.5% NaOCl. Perform the following
bleaching protocol:
1) Turn off the WCS and CH05 chiller, and disconnect the hoses from the front of the water
tank.
2) Start SonoLab and open the ‘Motion Control’ pane.
3) Fill the tank with approximately 450 ml of household Clorox and the rest with DI water.
4) Click the ‘Home’ button. The transducer will lower into the bath and the degas pump starts.
5) Operate the degas pump for approximately 5 minutes.
6) Click the ‘Service’ button. The transducer rises out of the bath and the degas pump stops.
7) Empty the bleach/water solution.
8) Place the empty water tank back into the instrument and click the ‘Home’ button. The
transducer lowers into the bath and the degas pump comes on purging the degas lines.
9) Click ‘Service’. The transducer rises out of the bath.
10) Empty the tank, rinse, and fill with fresh DI water.
11) Place the water tank back into the LE220.
12) Click ‘Home’. The transducer lowers into the bath and the degas pump starts. Allow pump
to operate for 3 or 4 minutes.
13) Click ‘Service’ to raise transducer out of bath and turn off pump.
14) Repeat steps 10 through 12 twice more.
15) Fill the tank with clean DI water, reconnect and power on the WCS and CH05 chiller. Allow
the water to degas for an hour prior to treating samples.
7.3
Transducer
Use care when handling the transducer. Permanent damage could result if anything is dropped onto
the face of the transducer or if the face of the transducer is damaged.
If drying or cleaning the transducer face, use a soft lint-free cloth. If broken glass must be removed
from the transducer surface, carefully pick any shards off the surface with tweezers. Do not rub
them away, as this may scratch the transducer surface.
7.4
Safety System
Test the system periodically. Verify that the acoustic treatments are disabled when the ‘Stop’ button
is pressed, or if the water level in the tank is too low. Verify that the door cannot be opened during
an acoustic treatment.
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WARNING: Any failure of the Safety System must be reported immediately to
Covaris. DO NOT attempt operation if the Safety System is malfunctioning.
7.5
Degassing System
The degassing pump should not be run without a water bath. Extended running without water will
cause the pump head to wear out. If water is low, the pump will shut off within 10 seconds.
When the pump is running, verify that there are small bubbles coming from the end of the outlet
nozzle. If you suspect that the system has become clogged, please contact Covaris for assistance.
There is a small inlet tube that can be removed and cleaned.
Degassing the instrument is best performed on room temperature water, as water below about 15
degrees C does not degas as efficiently. SonoLab prevents the chiller from coming on until the bath
has been degassing for 30 minutes. If being operated manually, the operator should, likewise, begin
degassing approximately 30 minutes prior to powering on the chiller. If it is necessary to have the
instrument available first thing in the morning, use SonoLab’s built in chiller and degas scheduler in
the Water Works section.
For long-term storage, water should be removed from the degassing pump lines. Follow the System
Shutdown procedure in Section 3.
7.6
Cleaning the System
Clean the external surfaces of the equipment as necessary with a damp lint-free cloth. Rinse the
water tank with clean water and wipe dry with a lint-free cloth.
CAUTION: Do not employ isopropyl alcohol, ammonia-based or abrasive cleaners on
the water tank, as these will damage the acrylic surfaces.
7.7
System Shutdown for Long Term (more than 2 weeks) Storage
7.7.1
Empty the water tank and purge the degas pump
Turn off the WCS and the CH05 Chiller.
Click on the Motion button to display the Motion Pane.
Click on ‘Service’. The degas pump will turn off and the transducer assembly will rise out of
the bath.
Empty the water bath and place it back in the instrument empty.
Click ‘Home’. The transducer will lower into the bath and the degas pump will come on,
purging the lines to the degas pump.
Click ‘Service’ when no additional water is coming from the lines, or when the pump shuts
off. The transducer will raise out of the bath.
Turn on the CH05 chiller, leaving it in stand-by mode. Allow it to run until no more water is
pumped into the bath from the WCS.
Empty the bath.
Rinse it with clean DI water and dry it with a soft, lint free cloth.
If instrument is being placed in indefinite storage, remove the particulate filter from the WCS.
Refer to instructions in the WCS manual as necessary. Note: Since the particulate filter will
remain wet internally even after draining, over a period of a couple weeks or more, some
biological growth may take place, potentially leading to filter blockage and reducing filter life.
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7.7.2
Close SonoLab
Exit SonoLab by clicking on the
displayed.
in the upper right corner. The following message is
Click “No” to cancel this request, or take no action and SonoLab will close following a 20 second
countdown. Click “Yes” to close the application immediately. A message asking if the
transducer should be moved to the Service Position is displayed. Clicking “Yes” causes the
transducer to be raised out of the water bath; clicking, “No” leaves the transducer where it is,
followed by the SonoLab software closing.
7.7.3
Turn System OFF
To shut down the laptop, select Shutdown from the START menu on the Windows screen and
select the option SHUTDOWN. Then the computer will power down.
Power down the instrument using the switch located near the power entry module of the
instrument, at the rear of the right side.
CAUTION: Leaving water in the water bath or degassing lines for an extended time
may promote algal growth in the tank and lines, This growth can interfere with water
quality and sample processing.
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8.0
TROUBLESHOOTING
This section provides a list of symptoms and recommended actions that may be taken to correct
simple problems. Please contact Covaris or your local representative for assistance with any
problem.
•
Instrument will not connect to SonoLab:
o Verify that the instrument is ON.
o Verify that the USB cable is connected at both ends and undamaged.
o Close and restart the SonoLab application.
o Go to Windows Device Manager (right click on Computer, then Properties) and verify
that Windows is recognizing the instrument, and that device drivers are properly
installed. Under Ports (COM & LPT), there should be four devices listed:
 Covaris RF Controller (COM3)
 Covaris RF Controller (COM4)
 Covaris RF Controller(Port3&4) (COM5)
 Covaris RF Controller(Port3&4) (COM6)
The COM assignments may be different from those listed above (e.g. COM 7, 8, 9. 10).
Under Universal Serial Bus Controllers, there should also be four devices listed:
 Covaris RF Controller Board
 Covaris RS-485 Port
 Covaris Serial Port 2
 Covaris Serial Port 3
Each of these devices should be listed. If any appear with a yellow “!” symbol, right-click
and try to re-install the device driver.
•
Safety Interlock or Sensor Failures (Water Level, Water Tank Present, Door Closed, Water
Temperature). These are generally failures that require the attention of a Service technician, but
please verify the following items before contacting Service:
o Water Level: Verify that the water level in the tank is at or above the “0” mark on the
WATER LEVEL label. Verify that the Acoustic Assembly is fully lowered into the tank.
o Tank Present: Verify that the Acoustic Assembly is fully lowered into the tank. Verify
that the tank is slid fully into the enclosure, so that the magnet embedded at the rear of
the tank can be sensed. Verify that the magnet is embedded in the rear of the tank.
o Door Closure not recognized: Verify that the door interlock is not bypassed.
o Water Temperature limits being reached, or inaccurate temperature readings: If the
water is running hot, verify that the chiller is operating and that it is circulating water
through the LE220-plus water bath. Program the set point of the chiller to a lower
temperature (select a method with a lower set point specified) and verify that the LE220plus bath get cools. Use a separate thermometer to verify the true temperature of the
bath.
•
Biological or chemical results (e.g. DNA shearing) not as expected:
o Verify that the average power values reported by SonoLab are approximately correct.
The typical average power is a few percent below the value “Peak Incident Power”
multiplied by “Duty Factor”. For example, if PIP=200 Watts and Duty Factor is 10%,
then an expected power reading would be approximately 15 to 17 Watts.
o Verify that the water temperature is correct and that the water is clear and free of algae
or other particulates. Perform a 10% solution bleaching protocol if this has not recently
been done.
o Verify that the degassing pump is operating (observe the pump outlet on the left side of
the tank – when the pump is running there should be occasional bubbles exiting the
pump outlet tube). Also examine the inlet “dip tube” visible from the right side of the
tank. The inlet holes (very small drilled holes in the side of the tube) should be free of
obstruction.
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o
•
•
If possible, please independently verify your analysis equipment. Covaris has DNA
shearing verification kits available. Please contact Covaris applications support or your
local representative for assistance.
SonoLab reported Power Level is lower or higher than expected:
o Verify that the average power values reported by SonoLab are approximately correct.
The typical average power is a few percent below the value “Peak Incident Power”
multiplied by “Duty Factor”. For example, if PIP=200 Watts and Duty Factor is 10%,
then an expected power reading would be approximately 15 to 17 Watts.
o Contact Covaris or your local representative for assistance.
Degas Pump problems:
o Verify that the water is clear and free of algae or other particulates. Perform a 10%
solution bleaching protocol if this has not recently been done.
o Verify that the degassing pump is operating (observe the pump outlet on the left side of
the tank – when the pump is running there should be occasional bubbles exiting the
pump outlet tube). Also examine the inlet “dip tube” visible from the right side of the
tank. The inlet holes (very small drilled holes in the side of the tube) should be free of
obstruction.
o If the degas pump makes a very loud noise when operating, it may need to be replaced.
•
Particulates in bath water
o Change the bath water with fresh DI water
o Perform a 10% solution bleaching protocol if this has not recently been done.
•
Tube Breakage
o Verify that the acoustic parameters being employed are correct.
o Verify that average power levels are approximately correct (see Power Level).
•
RF Board Limits Exceeded Error:
o If Peak Incident Power is set to values above 400 Watts, intense acoustic reflections
may cause an overload in the electronic circuits driving the transducer. To protect the
drive circuits and transducer, the system may shut down and post a message, “RF
Board Limits Exceeded RF Error is Present”. Should this error appear, please retry your
application with the following modifications:
 Fill the sample tube to eliminate splashing and large bubble formation in the
sample
 Replace the existing bath water with new, clean bath water
 Lower the PIP (Peak Incident Power) – this is often more effective than applying
very high power.
Repeat the method to verify that overload problems have been mitigated. If not, please
contact Covaris or your local representative.
Technical Support – On-going assistance with the operation or application of the equipment and/or
troubleshooting is provided via:
•
•
Telephone
o
United States: Tel: +1 781 932 3959 during the hours of 9:00am to
5:00pm, Monday through Friday, Eastern Standard Time (EST),
Greenwich Mean Time (GMT-05:00)
o
Europe: Tel: 44 (0) 845 872 0100, during the hours of 9:00am to
5:00pm, Monday through Friday, Greenwich Mean Time
E-mail queries to techsupport@covaris.com or applicationsupport@covaris.com.
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Appendix A: LE220-plus Series Site or Transport Preparation
Placement of Equipment
The area required for the instrument (see drawing below) is approximately 41” wide by 30” deep by
21” high (104cm x 76cm x 53cm).
Leave sufficient space beside the instrument and behind the instrument for cooling fan exhaust. Do
not block rear panel vents or fans.
The ultraCUBE may be placed under the bench.
Power Requirements
Provide at least four standard outlets, one for the laptop computer, one for the LE220-plus, one for
the CH05, and one for the WCS 2.0.
The LE220-plus system draws a maximum of 12.5A. All components are configured with universal
power supplies. Note: to avoid derating the CH05 cooling capacity, the CH05 may be used with a
step-up transformer if operated at less than 115 VAC.
WARNING: To prevent the possibility of electrical shock, always plug the
system into a grounded power source.
Water Requirements
The instrument’s water bath requires about 5.0 liters of either deionized or distilled water, which
should be topped up daily.
The water should be changed and bleaching protocol performed at least once a month.
Relocating of the System
When moving the instrument within a laboratory, or between nearby laboratories, please employ the
following guidelines:
•
Perform the System Shutdown process described earlier in this manual.
•
Close SonoLab and shut down the computer.
•
Disconnect power cords and USB cable. Disconnect the ultraCUBE.
•
Drain the water tank and reinstall in the instrument. Please contact Covaris or your local
representative for assistance.
•
Upon arrival at the new location, follow the Setup and Installation instructions described
earlier in this manual.
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Appendix B: Installing SonoLab Software, Firmware and Instrument Drivers
The E/LE220 system includes a laptop PC with SonoLab software and device drivers installed. If,
however, it is necessary to replace the computer and re-install SonoLab or device drivers, please refer to
the following instructions.
New copies of SonoLab, instrument drivers, user manuals, or protocols may be obtained from the
Covaris website www.covarisinc.com/resources/registered-users-login/E-Series/.
Please also contact Covaris technical support or your local representative for downloads of software,
drivers, manuals, or protocols.
Installing SonoLab
1. Begin with the computer ON and Windows running.
2. Download the appropriate SonoLab version from Covaris and extract the installer files to the
desktop.
3. Double-click “setup.exe” in the SonoLab 7 Installer folder to launch the software setup process.
4. If Windows asks if this is a trusted application, or if you wish to make changes, answer Yes. Accept
defaults when presented. When installation is complete, close the installer. A “Sonolab 7” shortcut
should appear on the computer desktop.
5. With the instrument powered ON, double click on the Sonolab 7 shortcut. SonoLab will open its
splash screen, as described in Section 3 of this manual.
Installing Well Plates (210062)
1. To install the standard set of plate definitions, open the installer and double-click the Setup
application. The Windows installer wizard performs the installation. Click the ‘Close’ button when
the ‘Successfully Installed’ message displays.
Installing Microsoft SQL Server Management Studio (210063)
1. To install the SQL Server suite, open the installer and double-click the Setup application. An
installer wizard will perform the installation. Accept defaults as prompted. Click the ‘Close’ button
when the ‘Successfully Installed’ message displays.
Install Windows Instrument Driver
When an instrument (with power ON) is connected by USB (Universal Serial Bus) for the first time,
Windows will identify the instrument by its Vendor and Product ID numbers and install specific device
drivers (software files required for communications with a USB device).
Windows will attempt to install these drivers in the following order:
If any E/LE220 has ever been connected to the computer, then a copy of the appropriate drivers will
be available to Windows locally, and Windows will automatically install these.
If Windows does not find a local copy of the necessary drivers, it will automatically search Windows
Update (assuming internet access is available via wireless link or Ethernet cable and Windows is
configured for automatic updates).
In either case, upon locating them, Windows will automatically install the drivers and pop up the
following message onto its desktop:
Drivers for several devices within the instrument will be installed sequentially. This may take several
minutes. If you click on the pop-up message, the status of these installations may be viewed.
Please allow all of these drivers to install and for Windows to confirm successful installation of all
drivers with the following message:
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Drivers are now installed and SonoLab will automatically connect with the instrument once started.
Manual driver installation:
If the automatic installation fails to occur, Windows will post the following message:
If restoring internet connection or automatic Windows Update fails to start the automated installation
process, then the following manual process will be required.
Click on the Windows Start button. Either via Control Panel, or by right-clicking on Computer and
choosing Properties, locate Device Manager. Click on this link to open the Device Manager
window. In the list of devices, locate Other devices and entries for RF Controller Board:
Under Other devices, right click first device RF Controller Board and select Update Driver
Software:
When the driver software update dialog opens, select the option to Browse my computer for driver
software:
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Point the file open dialog to “C:\Program Files\Covaris\Driver” and verify that “Include subfolders” is
checked:
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Click next. You may be notified that “Windows can’t verify the publisher of this driver software”. Click
on “Install the driver software anyway”:
Windows will install the driver and post the following dialog box with a message, “Windows has
successfully updated your driver software”.
Click the Close button. The Device Manager screen will refresh.
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Return to Other devices. Note that there is now a ‘USB Serial Port’ listed under the three ‘RF
Controller’ lines. Right-click on the ‘USB Serial Port’ and select ‘Update Driver Software…’, as
shown in the image below. Repeat the installation steps shown above.
Return to Other devices and repeat this process in order (top to bottom), for the 3 remaining
entries, until all Covaris device entries have been assigned a COM number under Ports (COM &
LPT). The ‘Device Manage’ window should now show:
Note that the ‘COM’ numbers will vary from computer to computer, but should occupy four
consecutive ports.
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Appendix C: Humidity and Water Management in the Covaris LE220-plus
The Covaris LE220-plus employs a six-liter tank of distilled or deionized water located inside the instrument
housing. For the system to operate correctly, the water must be filled to an appropriate level, must be
clean, free of algae growth, and degassed to retard bubble formation in the acoustic field outside of the
sample vessel. Additionally, bath temperature is controlled by the Covaris ultraCUBE connected to the
LE220-plus water bath. Managing this water and associated humidity is important for the correct operation
and reliability of your Covaris LE220-plus system. The following suggestions will help:
•
•
•
•
When the system is powered on, the interior is vented by fans located in the rear panel to maintain
an appropriate air temperature and humidity within the instrument enclosure. It is recommended,
when the acoustic tank is full, that the instrument be left with power on, so that these fans run and
retard water condensation on interior surfaces. If it is not practical or desirable to leave these fans
running when the instrument is not in use, please leave the front access door ajar so that interior air
may exchange with ambient air. The risk of condensate forming within the instrument is increased
when operating with a Water Conditioning System 2.0 (WCS), because the WCS will add a small
amount of heat to the bath water.
The WCS 2.0 precludes the need for a daily change of bath water. It is, however, important that
water which evaporates each day be made up with fresh distilled or deionized water. It is also
important that a monthly bleaching protocol continue to be followed, as the WCS 2.0 will retard but
cannot completely prevent algae from taking hold in hoses and interior tank surfaces. Detach the
WCS water flow from system when bleaching.
When the system is being operated at a low temperature, you may notice condensate water forming
on the black manifold block, on hoses leading to the degas pump, and around the fittings and
chassis of the WCS 2.0. Particularly in summer, when relative humidity is higher, condensate may
drip onto interior surfaces and small amounts may leak onto the laboratory bench. This
condensation is expected and no harm is being done to the system. Excess condensate dripping
onto a laboratory bench may be captured by placing an absorbent pad (e.g. VWR 82020-845)
beneath the system and/or WCS.
If the instrument is going to be idle for more than 2 weeks, it may be beneficial to turn all
components off to prevent any damage to the instrument. Please follow the recommended
sequence:
1. Turn off chiller
2. Move instrument to service position
3. Turn off the ultraCUBE and disconnect hoses
4. Empty the water tank
5. Replace the water tank and move instrument to load position
6. Purge the degas pump by turning on for 10 seconds. Purge the ultraCUBE lines by
connecting the hoses and powering on ultraCUBE until flow subsides
7. Turn the WCS off and disconnect hoses. Raise instrument to service position.
8. Empty the water tank and dry
9. Gently dry transducer and surfaces of instrument
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Appendix D: LE220R-plus
The LE220R-plus is designed to be integrated with a sample handling robot in an automated work cell. The
front door of the safety enclosure contains a cutout providing the robot arm access to the inside of the
LE220R-plus cabinet. The LE220R-plus presents the sample tray at the opening, and the robot arm loads or
unloads the sample through the opening. The LE220R-plus is controlled by custom software built using the
Covaris Integration Toolkit.
A light curtain has been added as an additional safety interlock. The light curtain is wired into a safety rated
relay controller the output of which is connected to the Covaris controller board. If an operator reaches
through the robot access opening while the system is running, motor power and acoustics are immediately
disabled.
The light curtain consists of invisible, infrared light and an infrared detector. Beams of light on 14mm
spacing are transmitted between the source and detector. Blocking one or more beams of light trigger the
safety interlock system. Acoustics and motion will remain paused until beams are unobstructed.
Light Curtain Mounted to the LE220R-plus
Robot Arm Accessing
LE220R-plus Sample Tray
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