A Bead Beating Lysis Instrument for use Anywhere in the WORLD!
“Take the Lab to the Field”
Catalog Number: S6022
For Research Use Only
Ver. 1.0.1
ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ www.zymoresearch.com
Page |2
PRECAUTIONARY INSTRUCTIONS
Be sure to read all safety instructions in this manual carefully and thoroughly. Pay attention
to warnings before using the TerraLyzer™. Failure to follow all instructions may lead to
injury.
Warning: Indicates a potential hazard which should be avoided.
Electrical Safety
Plug the Battery Charging Station into the Power Converter if using a 220V power source
Avoid contact with grounded surfaces
Outlets for charging must match the power tool plug – Adapters may be needed
Do not use the TerraLyzer™ in the rain or under wet conditions
Do not misuse the cord
Safety Equipment
 The TerraLyzer™ can be very loud, it is recommended to use ear protection during use.
For further safety information regarding the driver, battery, power converter, and charging
station please see accompanying literature.
It is recommended to keep the original packaging intact should you need to return the
product for any reason to your distributor or to Zymo Research Corporation.
Note: Zymo Research is not responsible for any harm or damage resulting directly/indirectly from the use of the TerraLyzer™ or
related product components, materials, or technologies.
Some reagents in the included trial kits are irritants. Wear protective gloves and eye protection and follow the safety guidelines
and rules enacted by your research institution or facility. Refer to product/reagent specific MSDS information available on our
website.
ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ www.zymoresearch.com
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CONTENTS
Introduction and Highlights
pg. 4
Product Contents
pg. 5
Diagram of the TerraLyzer™
pg. 6
TerraLyzer™ Operating Instructions
Battery Charging
pg. 7
Sample Processing
pgs. 7, 8
Cleaning
pg. 9
Anticipated Results
pg. 9
Warranty and Disclaimer Information
pg. 10
Environmental DNA Purification Technologies and Ordering Information
pg. 10
Popular Environmental DNA/RNA Purification Products from Zymo Research
pg. 11
“Take the Lab to the Field”
ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ www.zymoresearch.com
Page |4
INTRODUCTION
Degradation and contamination of biological samples
have been
obstacles to scientific study, and may be particularly problematic in
highly sensitive molecular-analysis techniques (e.g., PCR of low copy
DNA). Use of cryogenic freezing methods for environmental/forensic
sample preservation may often be too impractical to be employed.
The solution is the TerraLyzer™ and environmental DNA purification
kits from Zymo Research. The TerraLyzer™ is a cordless, hand-held
device developed for vigorous sample disruption (bead beating) that allows the
researcher/investigator to “Take the Lab to the Field” and to the site of sample collection.
The TerraLyzer™ can be used to lyse microbes in soil, sediment, sludge, and fecal samples and can
effectively process tough-to-lyse fungal, algal, plant, and animal tissues. It can be used at any remote
location and in most weather conditions when immediate sample collection, processing, and
preservation are required by the researcher. The device is compatible with most 2.0 ml tubes
containing lysis matrix, though ZR BashingBead™ Tubes should be used to obtain maximum yields of
DNA/RNA/Protein from tough-to-lyse and environmental sample sources.
The TerraLyzer™ consists of a cordless, electric drive unit mounted to a unique, proprietary lysis
chamber. It is designed to operate at full speed for as long as the trigger on the device is actuated
and charge remains in the battery. This allows the researcher to maintain precise control of the time
and extent of sample lysis.
Highlights
 For cordless, portable, and convenient processing of tough-to-lyse biological and
environmental samples.
 Allows the researcher to “Take the Lab to the Field” and to the site of sample collection with
Zymo Research’s state of the art products for environmental DNA and RNA purification.
 Ideal for both field and lab use.
In the field
or…
In the lab
ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ www.zymoresearch.com
P a g e |5
PRODUCT CONTENTS
1)
2)
3)
4)
5)
6)
7)
One (1) TerraLyzer™
One (1) Lithium Ion Rechargeable Battery
One (1) Battery Charging Station
One (1) Universal Power Converter
One (1) Zymo Research Timer
Two sets (4 total) Ear Plugs
Assorted Product Manuals and Literature
Also, to begin isolating DNA from environmental samples…
8) One (1) Trial-Sized (5 prep.) ZymoBIOMICS™ DNA Miniprep Kit w/ 0.1 & 0.5 mm
BashingBeads™
TerraLyzer™
Instrument
Battery Charging
Station
ZR BashingBead™ Lysis
Tubes (2.0 ml)
ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ www.zymoresearch.com
Page |6
DIAGRAM OF THE TERRALYZER™
Chamber Shield
Rotation Lock
Button
Lysis Chamber
Drive Unit
Trigger Switch
Battery
Indicator
Multi-position Head
Battery
ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ www.zymoresearch.com
P a g e |7
TerraLyzer™ Operating Instructions
Battery Charging
Connect the battery charging station to an appropriate power outlet. The charging station is
designed for 120 Volt, 60 Hz AC input.
A power converter has been included with the TerraLyzer™ for use with the charging station
outside of North America. Some regions will require an adapter to use the power converter
and should be provided by the user.
Place the battery in the charging station by aligning the raised ribs of the battery pack with
the slots in the charging station. Lights on the charging station should flash green while the
battery is charging. The battery pack is fully charged when the green lights stop flashing and
remain on.
A flickering red light while charging indicates a defective battery or faulty connection. Check
the connection of the battery with the charging station.
Sample Processing
 Sample Sources – The TerraLyzer™ device can be used to effectively lyse microbes in soil, sediment,
sludge, and fecal samples and can effectively process tough-to-lyse fungal, algal, plant, and animal
tissue. For best results, we recommend using skirted sample tubes like the ones provided with Zymo
Research’s environmental DNA and RNA purification kits. Tubes having conical bases are compatible
with the TerraLyzer™, however these may not hold up to the extreme pulverizing forces generated by
the device.
 Operating Temperatures – (Optimum -20oC to 45oC) Severe cold conditions can drain the battery and
effect the number of samples that can be processed with the TerraLyzer™. Keeping the battery warm
(e.g., in the pocket of a jacket or parka) prior to use can extend battery life in colder climates and
operating conditions.
 Processing Times – (Generally <3 minutes) The extent of sample lysis will vary according to the
processing time (see figure below). Due to the extreme pulverizing forces generated by the
TerraLyzer™, the temperature of samples can increase as a function of lysis time. This may affect the
integrity of DNA/RNA/protein released from biological material. If longer processing times (>3
minutes) are required, it may be necessary to let the sample cool down for a few minutes before
continuing with sample processing.
DNA (ng/µl)
80
60
DNA yield versus lysis time for yeast spore
samples processed with the TerraLyzer™.
Error bars represent one standard deviation
from three replicate measurements.
40
20
0
10
20
30
60
Lysis Time (seconds)
ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ www.zymoresearch.com
Page |8
TerraLyzer™ Operating Instructions
1. Attach the battery into the bottom of the TerraLyzer™ drive unit.
2. Depress the rotation lock button to adjust the TerraLyzer™ lysis chamber to the desired angle
(90°, 135°, or 180°) of operation. Release the lock button to set the angle; an audible snap will
indicate a secure setting. Use 180° for best results in a shorter time.
3. Make sure the screw top of a 2.0 ml lysis tube (e.g., ZR BeadBashing™ Tube) containing a sample
is tight before placing it into the lysis chamber of the TerraLyzer™ as shown below…
 Place the bottom of the lysis tube into the lysis chamber. Push the lysis tube down and
backward to “snap” the lysis tube into the lysis chamber. The tube will be secured by the two
set screws on either side of the chamber.
 Slide the chamber shield over and down to the base of the lysis chamber. The chamber shield
should sit below the stop screw at the top.
Stop Screw
4. Process the sample by depressing the red trigger for the desired time. Begin with a processing time
of 30 seconds [however, tougher tissues (e.g., plant, fibrous tissue) may require longer processing
times].
5. After processing, remove the chamber shield and take out the lysis tube. The lysed material
(lysate) can be used immediately for DNA/RNA isolation, or if processed using a stabilization
reagent (e.g. DNA/RNA Shield) it can be stored/transported for DNA isolation at a later time.
Please refer to specific product instructions for details concerning subsequent isolation of
DNA/RNA/Protein from lysed material.
ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ www.zymoresearch.com
P a g e |9
Cleaning the TerraLyzer™ Drive Unit and Lysis Chamber
Make sure the battery is disconnected from the TerraLyzer™ before cleaning. Wear protective
gloves (latex/nitrile) during all cleaning procedures.
Clean the TerraLyzer™ as necessary. The exterior of the TerraLyzer™ and the chamber shield can
be cleaned with a damp (water, dilute EtOH) paper towel or similar. Clean the lysis chamber by
wiping down the inner walls. Allow all components to air dry prior to use.
Should a lysis tube fracture or break in the sample chamber during processing, stop
processing and immediately clean it as recommended above.
Anticipated Results
M
The TerraLyzer™ effectively processes tough-to-lyse materials for subsequent high
yield DNA isolation. Following sample processing with the TerraLyzer™, DNA was
isolated from plant, soil, mouse, and insect tissue using specific Zymo Research
environmental DNA kits (pg. 11) and then separated in a 0.8% (w/v) TAE/agarose/EtBr
gel. DNA from source material is graphically represented above the gel image and “M”
is a 1 kb molecular weight DNA marker (Zymo Research)
ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ www.zymoresearch.com
P a g e | 10
“Take the Lab to the Field” and to the Site of Sample Collection with
Environmental DNA Purification Technologies from Zymo Research
Environmental DNA Miniprep Kits
ZymoBIOMICS™ DNA Miniprep Kit
Quick-DNA™ Fecal/Soil Microbe Miniprep Kit
Quick-DNA™ Fungal/Bacterial Miniprep Kit
Quick-DNA™ Plant/Seed Miniprep Kit
Quick-DNA™ Tissue/Insect Miniprep Kit
Cat. No. (50 preps.)
D4300
D6010
D6005
D6020
D6016
Stabilization Reagent
DNA/RNA Shield™
Cat. No.
R1100-50 & R1100-250
For Research Use Only
All of our products are intended for research use only. Not for use in diagnostic procedures.
Warranty
This product is guaranteed for one (1) year from the time of receipt. See below for repair/return policy.
Returns
For repairs made under warranty, the Goods must be returned to Zymo Research intact (non-tampered with, nondisassembled) with all components together and in their original packaging. Goods may not be returned for credit
except with Seller's (i.e., Zymo Research Corporation) permission, and then only in strict compliance with Seller's return
shipment instructions. Returned Goods (other than defective products) must be returned freight prepaid by the
Customer and will not be accepted without prior authorization of Zymo Research. The Goods must be returned in their
original packaging and in resaleable condition. Any returned items may be subject to a 20% processing (restocking) fee.
Terms of Purchase
We warrant to you, our direct customer, that our goods shall conform substantially to the description of such goods as
provided in our catalogs and literature accompanying the goods until their respective expiration dates. THIS WARRANTY
IS EXCLUSIVE, AND WE MAKE NO OTHER WARRANTY, EXPRESS OR IMPLIED, INCLUDING ANY IMPLIED WARRANTY OF
MERCHANTABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE. Our warranty shall not be effective if we determine, in
our sole discretion, that you have altered or misused the goods or have failed to use or store them in accordance with
instructions furnished by us. Our sole and exclusive liability and your exclusive remedy with respect to goods proved to
our satisfaction (applying analytical methods reasonably selected by us) to be defective or nonconforming shall be the
replacement of such goods free of charge, upon the return of such goods in accordance with our instructions, although
at our discretion we may provide a credit or refund. IN NO EVENT SHALL WE BE LIABLE UNDER ANY LEGAL THEORY
(INCLUDING BUT NOT LIMITED TO CONTRACT, NEGLIGENCE, STRICT LIABILITY IN TORT OR WARRANTY OF ANY KIND)
FOR ANY INDIRECT, SPECIAL, INCIDENTAL, CONSEQUENTIAL OR EXEMPLARY DAMAGES (INCLUDING BUT NOT LIMITED
TO LOST PROFITS), EVEN IF WE HAD NOTICE OF THE POSSIBILITY OF SUCH DAMAGES. If we manufacture custom goods
for you based on instructions, specifications, or other directions you provide to us, we shall not be liable for the lack of
sufficiency, fitness for purpose or quality of the goods to the extent attributable to such instructions, specifications, or
other directions. We shall not be liable for any loss, damage or penalty as a result of any delay in or failure to
manufacture, deliver or otherwise perform hereunder due to any cause beyond our reasonable control.
Patent/Trademark Disclaimer
Some technologies of the TerraLyzer™ are patent pending. Unless stated otherwise, no license or immunity under any
patents shall be either granted or implied by the sale of Zymo Research Corporation products.
ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ www.zymoresearch.com
P a g e | 11
Popular Environmental DNA/RNA Purification Products from Zymo Research
Product
Format
Kit Size
Cat No.
ZymoBIOMICS™ DNA Kits
MicroPrep. Bead Bashing, Spin Column Format (up to 5 µg/prep.)
MiniPrep. Bead Bashing, Spin Column Format (up to 25 µg/prep.)
96-Well Format. Bead Bashing (up to 5 µg/well)
96-Magnetic Bead Format. Bead Bashing (up to 10 µg/prep.)
50 preps.
50 preps.
2x96 preps.
2x96 preps.
D4301
D4300
D4303
D4302
Quick-DNA™ Fungal/Bacterial Kits
MicroPrep. Bead Bashing, Spin Column Format (up to 5 µg/prep.)
MiniPrep. Bead Bashing, Spin Column Format (up to 25 µg/prep.)
MidiPrep. Bead Bashing, Spin Column Format (up to 125 µg/prep.)
96-Well Format. Bead Bashing (up to 5 µg/well)
50 preps.
50 preps.
25 preps.
2x96 preps.
D6007
D6005
D6105
D6006
Quick-DNA™ Fecal/Soil Microbe Kits
MicroPrep. Bead Bashing, Spin Column Format (up to 5 µg/prep.)
MiniPrep. Bead Bashing, Spin Column Format (up to 25 µg/prep.)
MidiPrep. Bead Bashing, Spin Column Format (up to 125 µg/prep.)
96-Well Format. Bead Bashing (up to 5 µg/well)
50 preps.
50 preps.
25 preps.
2x96 preps.
D6012
D6010
D6110
D6011
Quick-DNA™ Tissue/Insect DNA Kits
MicroPrep. Bead Bashing, Spin Column Format (up to 5 µg/prep.)
MiniPrep. Bead Bashing, Spin Column Format (up to 25 µg/prep.)
96-Well Format. Bead Bashing (up to 5 µg/well)
50 preps.
50 preps.
2x96 preps.
D6015
D6016
D6017
ZR Plant & Seed DNA Kits
MiniPrep. Bead Bashing, Spin Column Format (up to 25 µg/prep.)
96-Well Format. Bead Bashing (up to 5 µg/well)
50 preps.
2x96 preps.
D6020
D6021
Environmental DNA Isolation
Environmental RNA Isolation
ZymoBIOMICS™ RNA Kit
Miniprep. Bead Bashing, Spin Column Format (up to 100 µg/prep.)
50 preps.
R2001
Quick-RNA™ Fungal/Bacterial Kits
MicroPrep. Bead Bashing, Spin Column Format (up to 5 µg/prep.)
MiniPrep. Bead Bashing, Spin Column Format (up to 25 µg/prep.)
50 preps.
50 preps.
R2010
R2014
Quick-RNA™ Tissue/ Insect
MicroPrep™
MicroPrep. Bead Bashing, Spin Column Format (up to 5 µg/prep.)
50 preps.
R2030
Quick-RNA™ Plant MiniPrep™
MiniPrep. Bead Bashing, Spin Column Format (up to 25 µg/prep.)
50 preps.
R2024
(Also, see our website at: www.zymoresearch.com for additional kit sizes and formats)
ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ www.zymoresearch.com
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