t Confocal Fluorescence Microscopy Systems (Page 1 of 4)

Imaging
t CHAPTERS
Laser Scanning
Microscopy
Microscopy
Components
OCT Imaging
Systems
Confocal Fluorescence Microscopy Systems (Page 1 of 4)
Features
Confocal Imaging Module for Inverted
and Upright Microscopes
n Complete Image Acquisition Software
Package Included
n Video-Rate Image Acquisition
(512 x 512 Pixels @ 30 fps)
n Capture Resolution
•2048x2048Pixels(Bi-Directional)
•4096x4096Pixels(Uni-Directional)
n Two- and Four-Channel Options
n Choice of Standard Multialkali PMTs
or High-Sensitivity GaAsP PMTs
n Optimizedfor400–750nm
n
OCT Components
Adaptive Optics
t SECTIONS
Tutorial
Multiphoton
Systems
Multiphoton
Accessories
Multiphoton
Essentials Kit
Confocal Systems
CLS-UVSS Two-Channel Fluorescence Confocal
System and MLS203 Stage Mounted on an Olympus
IX51 Microscope (Stage and Microscope Not Included)
Laser Scanning
Essentials Kit
Thorlabs’ Confocal Laser Scanning (CLS) Microscopy Systems
are comprised of compact, purpose built, imaging modules for
infinity-corrected, compound microscopes. They add the ability
to acquire high-resolution optical sections within a thick sample
or to reduce background fluorescence from a thin culture. The
CLS systems offer turnkey integration to almost any upright
or inverted microscope with access to an intermediate image
plane (e.g., a camera port) via a C-Mount thread. The included
software has an intuitive graphical interface that allows data to
be quickly recorded and reviewed while providing sophisticated
peripheral controls for image acquisition. All CLS systems are
user installable; however, on site installation is available.
All hardware components are directly controlled through the
ThorImageLSTM software, including automated Z stepping
control for optical sectioning (via piezo or stepper motor)
and automatic calculation of Airy disk units based on
objective magnification and pinhole size combination. Our
intuitive interface allows novice
and experienced users alike to
obtain high-resolution microscopic
images quickly and easily.
C. elegans motor neurons and muscle arms.
The figure shows the C. elegans strain, trIs30,
expressing YFP in body wall muscles (green)
andDsRED2intheventralnervecordand
motorneurons(red)CourtesyofDr.William
Ryu, University of Toronto.
1680
www.thorlabs.com
All CLS complete systems include
a multi-channel fiber-coupled laser
source, control electronics, Scan
Head, pinhole wheel, detectors,
and all fibers and cables needed
to interconnect the system.
Additionally, each system includes
a Windows® computer with a
24"monitor,dataacquisition,
and control boards as well as
ThorImageLSTM software. A
comprehensive installation and
operation manual is also included
with basic preventative maintenance
instructions to ensure that your
system performs optimally for years to come. Also available
are complete systems that combine the Thorlabs Confocal
package with third-party upright and inverted microscopes. For
further details on this convenient option, please contact us at
ImagingSales@thorlabs.com.
Scanner
At the heart of our systems is an efficiently designed Scan Head
that incorporates a resonant scanner and a galvanometer for fast
image acquisition. This allows for high imaging speeds up to
100framespersecond(at128x128pixelresolution)
orhighspatialresolutionimages(4096x4096
pixel resolution at 2 fps). At either extreme,
or anywhere in between, the control and
acquisition system creates high-quality, jitterfree images (see inset at left).
Scan Head
Located within the Scan Head is our
new, kinematic fluorescence filter
cube(DFMT1)forquickand
repeatable exchange of the primary
dichroic mirror. Our complete
systems come standard with a
primary dichroic that reflects four
laserlines(405,488,532,and642
nm). Other primary dichroics for use
with other wavelengths are available
upon request.
Optics
The scan lens assembly has been designed for superior imaging
performanceandiscolorcorrectedfrom400–750nm.This
broad range adds to the functionality of the system, enabling
theuseoflasersourcesdownto400nmwhilecolorcorrecting
fluorescence emissions from even the deepest of red-emitting
fluorophores. Coupled with ultra-sensitive, low-noise detectors and
control electronics, we are able to provide systems that redefine
the boundaries of contrast, resolution, and imaging speed at an
affordable cost.
Imaging
CHAPTERS
Confocal Fluorescence Microscopy Systems (Page 2 of 4)
Emission
Pinhole: Anautomated16-aperturepinholeassemblywithaperturesrangingfromØ25µmtoØ2mm,enablestheultimate
balance between resolution and signal (for further details, see the Laser Scanning Microscopy Tutorial on page XXX). The pinhole
isconvenientlypoweredandcontrolledthroughUSB.Additionally,themotorized,encodedcontrolofthepinholeensuresperfect
alignment and vibration-free movement. The emission light is focused on the pinhole and then collected by a large-core multimode
fiber for transmission to the PMT detector system.
Detector: Our systems provide two different detector options. The standard sensitivity multi-alkali PMTs provide a low-noise, highdynamic-range image that is appropriate for most life-science and industrial applications. If needed for weak or highly photosensitive
samples, we also offer an option with
DETECTORS
STANDARD SENSITIVITY
HIGH-SENSITIVITY
high-sensitivity,TEC-cooledGaAsP
Photocathode
Multi-Alkali PMTs
Gallium Arsenide Phosphide (GaAsP) PMTs
PMTs. With either choice, the gain of
Sensitivity
105 mA/W
176mA/W
the detector as well as the dynamic range
DetectionWavelengthRange
185–900nm
300–720nm
of the digitizer is controlled within the
ThorImage software.
Confocal LSM Images of Bovine Pulmonary Artery
Endothelial Cells
Bovinepulmonaryarteryendothelialcells
visualizedwithBODIPY®FL goat anti-mouse
IgG.ThenucleiwerecounterstainedwithDAPI.
ScannedAreaSize:600µmx600µm.
LaserSource:405nmand488nm.
ExpandablePMTModulesaredesignedformulti-channellaser
scanning microscopy applications.
Excitation
One of the great challenges in laser scanning microscopy
has been keeping the lasers in a multi-channel source
aligned and therefore at peak power. We have overcome this
problem by creating a four-channel laser source based on
four service-free fiber-pigtailed laser sources. Three of the
four wavelengths are combined into a single fiber using an
advanced, fully integrated fiber optic device. This solid state
coupling method provides lifetime, adjustment-free service
from our laser source.
The combined visible output is contained in a single mode
fiberwithanFC/PCconnector.Theoptional405nmlaser
output, which is delivered on its own single mode fiber,
is combined after the beam expander in the Scan Head
module. This allows the UV light to be coupled into the
lightpathwitha4mmbeamdiameter,therebyincreases
stability and maintaining the color correction of the system.
532 nm
642 nm
CH 2
ENABLE
CH 3
ENABLE
CH 4
ENABLE
Resonant
Scanner
Filter
Block
Galvo
Scanner
Scan Lens
Tutorial
Multiphoton
Systems
Multiphoton
Accessories
Multiphoton
Essentials Kit
Confocal Systems
Laser Scanning
Essentials Kit
Multimode
Fiber
PMT Module
Pinhole Wheel
Intermediate
Image Plane
Microscope
Tube Lens
Fiber Collimator
Emission Filter
Infinity-Corrected
Objective
SOURCE
SELECT
SECTIONS t
Scan Head
Pigtailed Lasers
SYSTEM
ENABLE
Adaptive Optics
Laser Input
Single Mode Fiber
Fiber Coupler
CH 1
ENABLE
OCT Components
Schematic Diagram of Confocal Laser Scanning Microscope
Schematic Diagram of Four-Channel CLS Laser Source
488 nm
Laser Scanning
Microscopy
Microscopy
Components
OCT Imaging
Systems
Bovinepulmonaryarteryendothelialcells
stained with a combination of fluorescent dyes.
Mitochondria were labeled with red-fluorescent
MitoTracker® Red CMXPos, F-actin was stained
using green-fluorescent Alexa Fluor® 488phalloidin.
ScannedAreaSize:600µmx600µm.
SingleLaserSource:488nm.
We offer four standard wavelengths in our laser source
(405,488,532,and642nm);othersareavailableupon
request.TheentirelasersourceiscontrolledbyasingleUSB
connection, which allows the user to turn each laser on and
off as well as to control its intensity.
405 nm
t
Primary Beamsplitter
Emission Dichroic
WAVELENGTH
Sample
POWER
Z Stage
www.thorlabs.com
1681
Imaging
t CHAPTERS
Laser Scanning
Microscopy
Microscopy
Components
OCT Imaging
Systems
Confocal Fluorescence Microscopy Systems (Page 3 of 4)
ITEM#
CLS-2SS
CLS-2HS
CLS-4SS
CLS-4HS
CLS-UV
3
3
3
3
3
3
3
3
3
3
3
3
3
3
Excitation
405nm
488nm
Laser Wavelengths - Additional
Wavelengths Available Upon Request
OCT Components
532 nm
642nm
Adaptive Optics
PrimaryDichroicMirror
3
QuadBandDichroicBeamsplitter
Scanning
t SECTIONS
Scanning Speed @ Resolution
100fps@128x128pixels(Max);2fps@4096x4096pixels(Min)
Scanner
Tutorial
X:7.8kHzResonantScanner;Y:GalvanometerScanMirror
Scan Zoom
Multiphoton
Systems
1X-8X(Approximately)
Capture Resolution
Upto2048x2048Bi-DirectionalAcquisition;Upto4096x4096Uni-DirectionalAcquisition
Diffraction-LimitedFieldofView(FOV)
Multiphoton
Accessories
FN25*=442µmx442µmFOV@40X;FN23*=407µmx407µmFOV@40X
Emission
Multiphoton
Essentials Kit
Standard Sensitivity
Number of
PMTs Included
Confocal Systems
Laser Scanning
Essentials Kit
2
High Sensitivity
2
4
447nm/60nmBandpass
3
3
514nm/30nmBandpass
3
3
3
3
3
3
3
3
3
3
3
3
3
3
525nm/50nmBandpass
EmissionFilters-CWL/FWHM
4
2
3
3
559nm/34nmBandpass
645nm/Longpass
3
3
495nm
562nm
SecondaryDichroic-CWL
3
605nm
3
3
3
3
*Field Number (FN) is the diameter of the image, formed at the intermediate image plane: FN = FOV * Magnification
Scan Head and Optics Assembly (CLS-UV)
UV Laser In
4.48"
(113.9 mm)
1.82"
(46.1 mm)
5.07"
(128.8 mm)
Z-Axis Options for Recording Optical Sections
Analog Control:AllofourCLSscancontrolmodulesincludea0–10Voltanalog
output that can be controlled digitally from within the ThorImageLSTM software. The
graphic interface allows the scaling of the output to be calibrated to the step size of any
externally controlled focus device such as a piezo objective mover.
Z-Focus Motor: We have designed a universal focus motor (MFC1) that mounts to the
fine focus knob of a commercially available microscope. Please see the specifications on the
next page for more information.
Piezo Z-Stage:TheMZS500-E500µmpiezoz-stagecanalsobecontrolledfromwithin
the ThorImageLSTM application to provide high-resolution Z sectioning of samples. The
MZS500-Eoffers500µmoftravelwithaminimumstepsizeof25nm.Pleaseseepage
1690formoreinformation.
Top View
9.93"
(252.2 mm)
VIS Laser In
Scan
Head
CONFOCAL LASER SCANNER
To
Detectors
12.89"
(327.3 mm)
11.38"
(289.0 mm)
Scan Lens
Front View
1682
www.thorlabs.com
Motorized
Pinhole
Wheel
Right Side View
We offer a variety of standard system configurations to address the
application-specific needs and budgetary constraints of our customers.
Aside from the standard
configurations outlined above,
TheDFMSeriesof
filter cubes is compatible
we are also able to utilize our
with Thorlabs’ Laser
broad resources and breadth
Scanning Microscopy
products as well as
of knowledge to provide fully
SM1 and 30 mm cage
customized systems that address
systems.
your specific requirements. Our
strength lies in the fact that
we are a vertically integrated
organization, able to leverage
the knowledge and technologies
of other Thorlabs divisions to
provide a fully integrated system
at an unparalleled price.
Updated
LF
3/28/12
Imaging
CHAPTERS
Confocal Fluorescence Microscopy Systems (Page 4 of 4)
Laser Scanning
Microscopy
Microscopy
Components
OCT Imaging
Systems
Thorlabs’ CLS Systems on Assorted Microscopes
Inverted Microscope
Configuration
Upright Microscope
Configuration
t
Thorlabs’
T-Scope
Configuration
OCT Components
Adaptive Optics
CLS-UV
CLS-FS
CLS-UV
SECTIONS t
Tutorial
Multiphoton
Systems
Multiphoton
Accessories
Multiphoton
Essentials Kit
Confocal Systems
Laser Scanning
Essentials Kit
All Thorlabs CLS Confocal Laser Scanning Systems can be mounted on inverted and upright commercial microscopes on
a standard C-mount camera port. For applications that do not require a commercial microscope, these systems are also
compatible with Thorlabs’ T-Scopes. Thorlabs is able to offer a complete confocal imaging solution with motorized control and
synchronization for Z stack image reconstruction with the use of the Motorized T-Scope. See page XXX for details.
ITEM #
CLS-2SS
$
CALL
CLS-2HS
CLS-4HS
CLS-4SS
CLS-UV
£
CALL
€
CALL
RMB
CALL
DESCRIPTION
Two-Channel Fluorescence Confocal System with Standard Sensitivity PMTs
CALL
CALL
CALL
CALL
Two-Channel Fluorescence Confocal System with High-Sensitivity PMTs
CALL
CALL
CALL
CALL
Four-Channel Fluorescence Confocal System with High-Sensitivity PMTs
CALL
CALL
CALL
CALL
Four-Channel Fluorescence Confocal System with Standard Sensitivity PMTs
CALL
CALL
CALL
CALL
Two-Channel UV Confocal System with Standard Sensitivity PMTs
Motorized Microscope Focus Controller
The MFC1 Motorized Microscope Focus Controller is a compact module
enabling motorized focus control of commercial optical microscopes. An
encoded stepper motor drive ensures repeatable positioning through the fine
focus drive of the microscope and provides positional information, even if the
fineadjustmentisdonemanually.TheunitiscontrolledviaUSBwithour
ThorImageLSTM software, instantly improving the functionality
of your equipment. Please
see page XXX to order a
threaded breadboard to fit
your application.
MFC1
Posts and Post
Holders Included
Features
Incremental Step: 100 nm (Minimum)
USBControlled
n EncodedStepperMotorDrive
n Controlled Through ThorImageLSTM Software
n
n
ITEM #
MFC1
$
$ 1,850.00
£
£ 1,332.00
€
€
1.609,50
MFC1 Mounted on an
Inverted Microscope
RMB
¥ 14,744.50
DESCRIPTION
MotorizedMicroscopeFocusController
www.thorlabs.com
1683
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