Instruction Manual - NBT New Bio Technology Ltd

VC-8 Manual, Rev. 050817
1
Warner Instruments
Perfusion Valve Controller
Model VC-8
Warner Instruments
1125 Dixwell Avenue, Hamden, CT 06514
(800) 599-4203 / (203) 776-0664
(203) 776-1278 - fax
VC-8 Manual, Rev. 050817
2
Table of Contents
SETUP.......................................................................................................................................................... 4
Valve System ........................................................................................................................................... 4
Overflow sensor ...................................................................................................................................... 5
VALVE CONTROLLER CONTROL DESCRIPTION ......................................................................... 6
Front panel .............................................................................................................................................. 6
TTL Inputs ............................................................................................................................................ 6
Command input toggle ......................................................................................................................... 7
Overflow ............................................................................................................................................... 7
Rear Panel ............................................................................................................................................... 7
D Connector ......................................................................................................................................... 7
Overflow In........................................................................................................................................... 7
Standby In............................................................................................................................................. 7
Ext Analog In........................................................................................................................................ 7
Event Out.............................................................................................................................................. 8
Power input module ............................................................................................................................. 8
INSTRUCTIONS FOR USE ...................................................................................................................... 8
Flow Adjustment .................................................................................................................................... 8
APPENDIX................................................................................................................................................ 10
Maintenance.......................................................................................................................................... 10
Cleaning ............................................................................................................................................. 10
Warranty ............................................................................................................................................... 10
Specifications......................................................................................................................................... 11
Certifications......................................................................................................................................... 12
Warner Instruments
A Harvard Apparatus Company
VC-8 Manual, Rev. 050817
3
The VC-8 Valve Control System lies at the heart of a multi-valve perfusion system designed to
automate and control the delivery of solutions to Warner Instruments imaging and recording
chambers. However, its flexible design allows the system to be used in many applications not
using Warner equipment.
Features Include:
9
9
Choice of 3 valve types
8 channel system
9
9
Spill sensor
Manual and computer control
The complete system includes the valve controller, a valve bracket with valves, connecting
cable, C-Flex® tubing, an MP Series manifold, a ring stand, eight 60 cc syringes, 25 feet of
Tygon tubing and an assortment of tubing connectors.
A new and exciting feature to the VC-8 system is the Spill Sensor Probe. This probe is used to
detect the presence of an overflow condition and, when activated, it will automatically shut off
all valves protecting your microscope optics.
The controller can independently regulate the function of up to eight valves. Individual valves
can be controlled via a manual switch, an external analog signal or an external digital (TTL)
signal. An event marker pulse, generated each time a valve is switched on, is provided at the
rear of the instrument for recording into your acquisition system. Valve transitions (opened or
closed) occur at full power to insure rapid response times and are then held in place at less
than half power to prevent heat transfer to solutions.
Valve options are pinch, Teflon, and Lee miniature types. Pinch valves are supplied as standard
equipment. They are the simplest from a maintenance standpoint since solutions do not come
into contact with the valve body and the tubing is easily exchanged. Pinch valves are dual
acting with both normally open and normally closed sides. If the valve input line is split using a
Y-connector, solution can flow to waste while the valve is in the off position.
Pinch valves are designed to work with silicone, C-Flex®, or other similar type tubing. All
systems are supplied with a small quantity of C-Flex® tubing. Response times for these valves
varies with solution viscosity and tubing. However, typical times are 20-50 ms.
NOTE: Minimizing the length of C-Flex® or silicone tubing used will reduce the gas permeability
of the system.
Warner Instruments
A Harvard Apparatus Company
VC-8 Manual, Rev. 050817
4
SETUP
Before beginning setup take inventory of the plastic fittings supplied with the complete
system. These fittings include six (8) each of 1/16” ID tube-to-tube connectors, 1/16” ID tubeto-tube Y connectors, 1/16” ID Luer-to-female barb connectors, and stopcock-with-Luer
connector. Connectors are shown, in order, below. Plastic tubing can be attached to these
connectors with little effort.
Valve System
Figures 1a and 1b below illustrate two configurations wherein the perfusion system
operates in either a stopped flow or continuous flow-to-waste mode. In general, the shortest
response time of the system to selection of a solution will be achieved by keeping the tubing
length between the MANIFOLD and sample as short as possible.
Warner Instruments
A Harvard Apparatus Company
VC-8 Manual, Rev. 050817
The VC-8 Valve Controller is initially assembled by first attaching the
STAND as shown in Figure 2 (below).
5
VALVE BRACKET
to the
RING
This is followed by insertion of C-Flex® tubing into the pinch valves. Depending on the flow
arrangement you’re using, cut eight (8) or sixteen (16) pieces of C-Flex® tubing to length. Attach
a tube-to-tube connector to each end if using the system shown in Figure 1a. Attach a tube-totube connector to only the lower end if using the system shown in Figure 1b. Mount the tubing
to the valves as shown in Figure 1.
NOTES: (1) A slight stretch of the tubing while mounting will facilitate insertion into the pinch
valve. Keep the length of C-Flex® tubing as short as is feasible. (2) The solenoid (pinch) valves
will not transition between open and closed states prior to insertion of the C-Flex® tubing.
Next attach the SYRINGE HOLDER to the RING STAND. Affix a stopcock-with-Luer connector to
each syringe and mount into the SYRINGE HOLDER. Now cut six (8) pieces of Tygon® tubing just
long enough to run from the stopcock to the C-Flex® tubing. Attach a Luer-to-female barb
connector to the top end and a tube-to-tube connector to the valve end and make attachments
to the syringe and for each channel.
Finally, attach a tube-to tube connector to the C-Flex® tubing on the outboard end of the
valve and attach a short piece of Tygon® tubing. Insert a short length of PE-160 tubing into the
Tygon® tubing and make a connection to the manifold.
Overflow sensor
An overflow or spill sensor is has been provided to assist you in providing a level of
protection to your microscope while using the VC-8 Valve Control System. The overflow sensor
itself is a Ag/AgCl probe which grounds through the chamber bath. A resistance of 10 MΩ or
less between the probe and bath will trigger the overflow circuitry.
Place the probe adjacent your perfusion chamber such that the maximum solution height
(say, just prior to an actual overflow) is achieved when the solution contacts the tip of the
Warner Instruments
A Harvard Apparatus Company
VC-8 Manual, Rev. 050817
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probe. Contact between the probe and your solution will activate the overflow circuitry and
close all the valves. The OVERFLOW SENSOR plugs into the OVERFLOW IN BNC on the rear of the
controller.
VALVE CONTROLLER CONTROL DESCRIPTION
Front panel
The front panel contains TTL inputs for each valve, a 3-position toggle switch for manually
setting the state of each valve and an LED displaying the active status for each valve. There is
also a 2-position toggle switch for selecting the command input mode, a SPILL SENSOR indicator,
and a power switch with power on LED.
TTL Inputs
TTL inputs (front panel BNC’s and ON/OFF TOGGLE SWITCHES) are provided for all 8 channels
of the VC-8 controller. Operation of the front panel TTL inputs is selected by placing the 2
position COMMAND INPUT TOGGLE into external digital mode. Use of these inputs can allow the
opening of more than one valve at the same time from a digital source such as your computer.
Placing the ON/OFF TOGGLE SWITCH into the on position places the associated valve into its
open state. A lit LED indicates the open state of the valve. This setting is overridden by the
overflow sensor.
Placing the ON/OFF TOGGLE SWITCH into the off position places the valve into its closed state.
An unlit LED indicates the closed state of the valve.
Placing the ON/OFF TOGGLE SWITCH into the ext position activates the associated BNC
inputs. A logic level low (0 V) applied to the BNC places the associated valve into the closed
state. Correspondingly, a logic level high (3-5 V) places the associated valve into the open state.
A lit and an unlit LED indicates the open and closed states of the valve, respectively. These
settings can be overridden by the overflow sensor.
Warner Instruments
A Harvard Apparatus Company
VC-8 Manual, Rev. 050817
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Command input toggle
The COMMAND INPUT TOGGLE is used to select between the front panel external digital
controls and the rear panel external analog controls.
Overflow
The OVERFLOW LED indicates an overflow condition sensed by the OVERFLOW SENSOR.
Activation of this circuit by an overflow condition immediately overrides all other settings and
closes all valves.
Rear Panel
The rear panel contains the power input module with fuse, a 15 pin D-connector for the VALVE
SET, and 4 BNC connections for OVERFLOW IN, STANDBY IN, EXTERNAL ANALOG IN and EVENT OUT.
D Connector
A 15 pin "D" type female connector is used to connect the
CONTROLLER.
VALVE BRACKET
(and valves) to the
Overflow In
This BNC is used to connect the
position and plug it into this BNC.
OVERFLOW SENSOR
to the controller. Place the sensor into
Standby In
This BNC is provided to allow the placing of the VALVE CONTROLLER into standby mode.
Standby mode disables EXT ANALOG IN inputs and closes all valves.
Activation of this input is selected by placing the FRONT PANEL COMMAND input toggle into
external analog mode and a TTL level high (3-5 V) places the instrument in standby.
Ext Analog In
This BNC is used to operate valves using analog voltage settings. The voltage applied to
this input selects the valve to open. All other vales are closed. Switching voltages closes the
Warner Instruments
A Harvard Apparatus Company
VC-8 Manual, Rev. 050817
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open valve and open the other. Valve selections are overridden by the
the STANDBY BNC.
OVERFLOW SENSOR
and
The table below indicates the selection scheme.
Applied voltage (V)
Valve selection
0
standby
1
1
2
2
3
3
4
4
5
5
6
6
7
7
8
8
Event Out
The Event Marker output produces a 500 ms logic-level output (+5 V) each time a valve is
turned on. At all other times the Event Marker output is low (0 V).
Power input module
A polarized, 3-conductor, IEC320/CEE-22 connector is used for line (mains) power input
to the instrument. A removable cordset, terminated with a NEMA 5-15P connector, is standard.
A fuse holder contains a protective fuse in series with the high side (brown or black wire) of the
mains. The holder accepts 5 x 20 mm fuses of the type indicated.
INSTRUCTIONS FOR USE
Flow Adjustment
Flow rates can adjusted by raising or lowering the reservoir holder, as well as by adjusting
the height of each reservoir within the holder.
The table on the next page lists the approximate flow rates for a reservoir at the specified
height with the supplied Tygon® tubing.
Warner Instruments
A Harvard Apparatus Company
VC-8 Manual, Rev. 050817
9
Reservoir height
Approximate flow rate
61 cm (24 in.)
14 ml/min
30 cm (12 in.)
9 ml/min
20 cm (8 in.)
5 ml/min
Flow rates as a function of reservoir height.
Warner Instruments
A Harvard Apparatus Company
VC-8 Manual, Rev. 050817
10
APPENDIX
Maintenance
Cleaning
Do not use alcohol, aromatic hydrocarbons or chlorinated solvents for cleaning. They may
adversely react with the plastic materials used to manufacture the system. If salt solution spills
on the valve assembly it should be cleaned as soon as possible with a soft cloth dampened with
a mild solution of detergent and water.
NOTE: Teflon and Lee Mini Valves must be completely flushed with distilled water after each
use. Permanent damage will result if saline solution is allowed to crystallize inside the valve.
The exterior of the CONTROLLER may be cleaned periodically to remove dust, grease and
other contamination. It is not necessary to clean the inside. Use a soft cloth dampened with a
mild solution of detergent and water. Avoid abrasive cleaners.
Warranty
The VC-8 is warranted to be free from defects in materials and workmanship for a period of
two years from the date of shipment. If a failure occurs within this period, we will repair or
replace the faulty component(s) at our discretion. This warranty does not cover failure or
damage caused by physical abuse or electrical stress (e.g., exceeding specified input limits).
Shipping charges to the factory are the customer's responsibility. Return shipping of the
repaired unit will be paid by Warner Instruments.
Warner Instruments
A Harvard Apparatus Company
VC-8 Manual, Rev. 050817
11
Specifications
Valves
12 VDC /0.25 A to maintain pinch
Tubing
1/32 ID x 3/32 OD tubing
Valve Bracket
Delrin. Mounts on 3/8” or 1/2” ring stand
Connection Cable
2.4 meter (8 ft.) connecting cable terminated with quick disconnects on
valve end and 15 pin male "D" type connector on controller end.
Reservoirs
Reservoir Holder
60 cc capacity syringes
Delrin. Holds eight syringes with thumb screws for each reservoir.
Valve Controller
(front panel)
Power
Two position on/off with power-on LED
Input Select
Two position selecting analog or digital inputs
Switch Selection
On (manual), Off or External
External Input
+5 V TTL-compatible (BNC Connector)
Overflow
LED indicating overflow condition
Valve Controller
(rear panel)
Overflow In
BNC connection to OVERFLOW SENSOR. Triggered when sensed
resistance falls below 10 MΩ.
Standby In
BNC connection to activate standby mode. TTL high closes all valves.
Ext Analog In
BNC connection for valve selection. Active valve opened by analog
voltage.
Event Marker
Logic level pulse 500 ms nominal (BNC connector)
Manifold
2/1, 4/1 or 6/1 nominal dead space
Power
110-130 or 200-250 VAC, single-phase, 50/60 Hz, 20 watt
Operating Temperature
10-40° C (50-104° F)
Dimensions (H x W x D)
89 x 203 x 305 mm (3.5 x 8.0 x 12 in.)
Weight / Shipping Weight
3.7 kg (8 lb.) / 4.6 kg (10 lb.)
Operating conditions
Equipment is intended to be operated in a
controlled laboratory environment.
Temperature: 0-40 °C
Altitude: sea level to 2000 m
Relative humidity: 0-95%
Warner Instruments
A Harvard Apparatus Company
VC-8 Manual, Rev. 050817
12
Certifications
Declaration of Conformity
CE MARKING (EMC)
Application of Council Directive: 89/336/EEC
Standards To Which Conformity
Is Declared:
EN55022 Class A
EN61000-3-2
EN61000-3-3
EN50082-1:1992
EN61000-4-2
EN61000-4-3
ENV50204
EN610000-4-4
EN610000-4-8
EN610000-4-11
Manufacturer’s Name:
Warner Instruments, LLC
Manufacturer’s Address:
1125 Dixwell Avenue
Hamden, CT 06514
Tel: (203) 776-0664
Equipment Description:
Valve Controller
Equipment Class:
ITE-Class A
Model Numbers:
VC-8 Controller
I the undersigned, hereby declare that the equipment specified
above, conforms to the above Directive(s) and Standard(s).
Place: Hamden, Connecticut USA
Signature:
Full Name: Burton J. Warner
Position: President
Warner Instruments
A Harvard Apparatus Company
VC-8 Manual, Rev. 050817
13
Declaration of Conformity
CE MARKING (LVD)
Application of Council Directive: 73/23/EEC
Standards To Which Conformity Is
Declared:
EN61010-1:1993
Manufacturer’s Name:
Warner Instruments, LLC
Manufacturer’s Address:
1125 Dixwell Avenue
Hamden, CT 06514
Tel: (203) 776-0664
Equipment Description:
Equipment Class:
Valve Controller
Safety requirements for electrical
equipment for measurement and
laboratory use
Class I
Model Numbers:
VC-8 Controller
I the undersigned, hereby declare that the equipment specified
above, conforms to the above Directive(s) and Standard(s).
Place: Hamden, Connecticut USA
Signature:
Full Name: Burton J. Warner
Position: President
Warner Instruments
A Harvard Apparatus Company
VC-8 Manual, Rev. 050817
14
Warner Instruments
A Harvard Apparatus Company