The MIFETM system
for in-vivo non-invasive ion flux measurements in life science
Annex No. 1 – Technical Specification of Product
MIFE™ Overview
Membrane-transport processes underlie many essential cell biological processes and are central to perception
and signalling in response to virtually every known environmental factor. The MIFE™ system for non-invasive ion
flux measurements is a unique tool enabling in situ characterisation of the activity of major membrane
transporters in a wide range of living systems. See Newman IA (2001) Plant, Cell & Environment 24, 1-14.
The principle of the MIFE™ system is to measure the electrochemical potential gradient in solution near the tissue
specimen (see the figure). This is done by moving an ion-selective micro-electrode probe between two positions
near the specimen, and then converting the measured potential difference into net flux of the ion. The userfriendly interface allows convenient display, storage, and analysis of the recorded data.
The key features of the MIFE™ system are:
•
Non-invasiveness. Net ion fluxes can be
recorded from the specimen for many hours
or even days.
•
High temporal resolution. Net fluxes are
measured every few seconds, making the
MIFE™ an ideal tool to resolve rapid kinetics
of cellular responses to external stimuli.
•
High spatial resolution (several µm) allowing
fine mapping of net flux profiles from small
specimens (e.g. single cells).
•
Ability to measure neutral molecule flux.
Amperometric probes for oxygen, CO2 and
other molecules can be used.
•
Simultaneous measurements of several ions or neutrals. By assessing stoichiometry ratios between various
ions, valuable information can be gained about the membrane transporters involved.
MIFE3 Technical
The MIFE™ system was developed from 1987 and is made in Tasmania, Australia. From 2015, the new MIFE3
system comprises electronic hardware (main amplifier/controller, two 4-channel preamplifiers and Motor Drive),
with stepper motor-driven micromanipulators and suitable mountings for the manipulators. A suitable
microscope can also be incorporated, designed for horizontal measuring chamber (Inverted microscope), or for
vertical measuring chamber (replacing the stage of a microscope lying on its back).
The main amplifier is the lower enclosure in the following picture. Cables from the two preamplifiers are shown
at the front. The white USB cable to the computer is at the back right. Input sockets for low-impedance inputs
are also provided alongside. The motor-drive control electronics is in the upper enclosure.
Low voltage AC power input connects into the rear of the enclosure, but is hidden in the picture. The system
requires 10-12 VAC, 0.5 A, from a mains power pack (user supplied).
IMBROS 1059 Cambridge Road Cambridge Tasmania Australia 7170
Technology for Laboratory and Marine Science
ph (03) 6216 1500
page 1
info@imbros.com.au
The MIFETM system
for in-vivo non-invasive ion flux measurements in life science
Amplifier electrical characteristics
Each of two pre-amplifiers has 4 inputs. The input leads are shielded and insulated. The shields are not grounded
but are driven at the same voltage as the input signal.
Input resistance:
Maximum safe input voltage range:
Signal input voltage range:
Signal input “window” range:
Offset range to adjust the signal “window”:
Preamplifier gain:
Input sensitivity:
Source resistance measurable range:
Main amplifier gain:
>1012 Ω, with physical 1MΩ in series
+/- 5 V
+/- 500 mV
+/- 50 mV
+/- 500 mV
+ 9 (or unity, selected by a jumper)
1.5 μV for one LSB of D/A converter
1 MΩ to 10 GΩ
- 10
Manipulators and their control
Custom-mounted, Narishige-sourced manipulators are provided with the MIFE3 system. These are driven by a
custom-mounted stepper motor that is controlled by the MIFE3 Motor Drive box (on top in the above picture).
For Horizontal Chamber configuration, the manipulators are supplied mounted together, ready for placement in
the experimental cage.
For Vertical Chamber configuration, the manipulators are supplied mounted on the microscope that is also
supplied. Some users may prefer to supply their own microscope.
IMBROS 1059 Cambridge Road Cambridge Tasmania Australia 7170
Technology for Laboratory and Marine Science
ph (03) 6216 1500
page 2
info@imbros.com.au
The MIFETM system
for in-vivo non-invasive ion flux measurements in life science
Motor Drive enclosure
Power source: User supplied mains plug pack, 15-16 VAC or 22-24 VDC, 1.25 A
Power switch and indicator light on front panel
Motor Enable switch on front panel
Output to motor is from a socket on back panel to a connector on the mounted stepper motor. Its black cord is
seen in the picture above.
Control input from computer: USB connector on back panel.
Computer (PC)
The computer may be provided by the user. Minimum requirements are as follows:
Processor:
Operating system:
Memory:
Hard drive:
3 USB ports:
Duo core processor. Minimum: 1.0 GHz. 2.0 GHz or faster recommended
Windows 7 SP1 or above.
Minimum 1GB for MIFE3. 2GB or more recommended
Minimum 12GB free disk space for MIFE3
1 for data acquisition
1 for motor drive
1 for data transfer
MIFE3 Software
The MIFE3 software and its installation instructions are supplied directly to the end user.
The MIFE3 software provides interfaces for easy configuring and controlling the MIFE3 hardware, for data
capture, display and analysis. A MIFE3 User Guide is provided as a file in the program folder after the MIFE3
program is installed.
The MIFE3 system is sold by IMBROS, in arrangement with the University of Tasmania.
Author: Ian Newman; ian.newman@utas.edu.au
Copyright © University of Tasmania, Private Bag 37, GPO Hobart Tasmania 7001, Australia.
IMBROS 1059 Cambridge Road Cambridge Tasmania Australia 7170
Technology for Laboratory and Marine Science
ph (03) 6216 1500
page 3
info@imbros.com.au
MIFE3 Technical Specifications, requirements
October 2016
Introduction
The MIFE system was developed from 1987 and is made within the School of Mathematics and Physics
in the University of Tasmania. It is used for the measurement of net fluxes of ions and neutral molecules
near tissues in solution. From 2015, the new MIFE3 system comprises electronic hardware (main
amplifier/controller, two 4-channel preamplifiers and Motor Drive), with stepper motor-driven
micromanipulators and suitable mountings for the manipulators. The MIFE3 software is used for system
control, data acquisition and analysis. The user provides the microscope of choice, designed for
horizontal measuring chamber (Inverted microscope), or for vertical measuring chamber (replacing the
stage of a microscope lying on its back).
Hardware
Main amplifier physical characteristics and connectors
The metal case (Green terminal on the rear panel) is the system and shielding earth. It should be
connected to a good earth point.
AC power input, 10 to 12 VRMS, 0.5 A, from a mains power pack (user supplied).
Back panel USB connector for the computer.
On front panel: Power switch and indicator light.
Two D15P connectors for inputs from preamplifiers. The left one is for the “A” channels 1 – 4, the right
one for the “B” channels 5 – 8. Total gain for each channel, with preamplifier (9) and main
amplifier (-10) is about - 90.
Two DB9S connectors for direct, low impedance, input to the 8 channels. With gain of - 10, the signals
sum with the signals from the preamplifiers. Pin Connections: Pins 1, 2, 3: Analog ground. Pin 4:
+6V. Pin 5: -6V. Pin 6: Chan 1(5). Pin 7: Ch 2(6). Pin 8: Ch 3(7). Pin 9: Ch 4(8).
Preamplifier (each of two) physical characteristics
4 inputs. The input leads are shielded and insulated; the shields are not grounded but are driven at the
same voltage as the input signal.
Signal reference (analog common), referred to as “ground”, at green terminal and four small sockets on
the back. For safety, when any input lead is not in use, it should be plugged into a small socket.
Cable to main amplifier (channels 1-4 or 5-8).
The case is connected, via the cable shielding, to main amplifier chassis and thence to system earth.
Signal input characteristics and specifications (each of 8 channels)
Input resistance
Maximum safe input voltage range
Signal input voltage range
Signal input “window” range
Offset range to adjust the signal “window”
Preamplifier gain:
MIFE3_SystemSpecs.doc
1
>1012 Ω , with physical 1 M Ω in series.
+/- 5 volt
+/- 500 mV
+/- 50 mV
+/- 500 mV
+ 9 (OR unity, selected by a jumper).
13 December, 2017
Input sensitivity
Source resistance measurable range
Main amplifier gain
Signal bandwidth
Data sampling rate (typical)
Low impedance inputs to the main amplifier
Output
and
1.5 μV for one LSB of D/A converter
1 MΩ to 10 GΩ
- 10
10 Hz (Set by an R-C filter roll-off)
<0.1 Hz to 20 Hz
sum with preamplifier output signals
Digital via USB to the computer
Analog is available internally if requested.
Computer - this is provided by the user. The following are minimum requirements.
Processor: Duo core processor or faster. Minimum: 1.0 GHz. Recommended: 2.0 GHz or faster.
Operating system: Windows 7 SP1 and above.
Memory: Minimum: 1GB for MIFE3. Recommended: 2GB or more
Hard drive: Minimum: 12GB free disk space for MIFE3.
2 USB ports for data acquisition (the main amplifier, and motor drive)
1 USB port for data transfer
Manipulators and their control
Custom-mounted, Narishige-sourced manipulators are provided with the MIFE system.
These are driven by a custom-mounted stepper motor that is driven by the MIFE Motor Drive box.
For Horizontal Chamber configuration: MX-2; MHW-4-1 & SM-17. These are supplied mounted.
It is possible to use MHW-4 (5:1 movement) or the three axis MHW-103 in place of MHW-4-1.
For Vertical Chamber configuration: MMT-5 & MWS-1A. Both these are to be mounted by the user.
The three axis MHW-103 may be chosen in place of the MWS-1A. Both are 1:1 movement.
Motor Drive box electrical characteristics: Power source: User supplied mains plug pack, AC 15-16 V or DC 22-24 V, 1.25 A
Power switch and indicator light on front panel
Motor Enable switch on front panel
Output to motor: D25P socket on back panel
Control input from computer: USB connector on back panel.
Grounding
System and shielding earth
This is the large green terminal at the back of the MIFE3 amplifier, which is connected to the amplifier
and preamplifier cases and to the shielding of the cables to the preamplifiers. This green terminal must
be connected to a good earth point to fulfil the basic grounding and shielding requirements.
The electrometer bench, shielding, motor base and also the computer chassis should all be earthed by
connecting them to the same earth point or to this green terminal.
Analog common
This is the “ground” reference for the analog signals in the analog amplifier circuitry. It is connected to
the green terminal on each preamplifier. The “bath” reference electrode connection should be made to
this terminal and kept isolated from the shielding or cage. This will minimise digital or pickup noise
currents in the analog common line that produce false “signals”. In good locations it may not cause
noise if the analog common is in contact with the chassis or shielding earth, but it is best practice to
keep the signal ground separate from the system earth.
MIFE3_SystemSpecs.doc
2
13 December, 2017
Software
The MIFE3 software and installation instructions are supplied to the purchaser on a DVD.
MIFE3 software provides interfaces for easy configuring and controlling the MIFE3 hardware, for data
capture, display and analysis. A MIFE3 User Guide is provided as a file on the DVD and in the program
folder after the MIFE3 program is installed.
Author: Ian Newman; ian.newman@utas.edu.au
Copyright © University of Tasmania, Private Bag 37, GPO Hobart Tasmania 7001, Australia.
MIFE3_SystemSpecs.doc
3
13 December, 2017
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Optical/Mechanical Data
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manual via quadruple objective nosepiece
Objectives
infinity-corrected objective range with W 0.8 mounting thread
Plan-Achromat:
4x/0.1, 4x/0.1 Ph0, 10x/0.25 Ph1
LD-Plan-Achromat: 20x/0.3 Ph1, 40x/0.5 Ph1, 20x/0.3 Ph2, 40x/0.5 Ph 2
Phase-Slider
Universal Phase slider for the objectives Ph1: convenient and economical
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Eyepieces
with field-of-view number 20
30 mm tube diameter
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Specimen stage
Dimensions (width x depth)
Specimen guide
Verniers with numerical and alphabetic scale
Coaxial drive
fixed
200 x 239 mm
right side
X direction: numerical scale, readable from right to left
Y direction: alphabetic scale, readable in the mirror right side
LD condenser 0.3
for Vobj 4x to 40x, a = 72 mm
LD condenser 0.4
for Vobj 4x to 40x, a = 55 mm
Binocular tube 45°/20
Maximum field-of-view number
Interpupillary distance
Tube angle
Viewing height
Viewing port
20
adjustable from 48 to 75 mm
45°
360 to 397 mm
tube factor 1x
Trinocular (photo)tube 45°/20
Maximum field-of-view number
Interpupillary distance
Tube angle Viewing
height Viewing port
Photo/video port
Fixed beam splitting
Light source
20
adjustable from 48 to 75 mm
45°
360 to 397 mm
tube factor 1x
tube factor 1x, 60 mm C-mount
50 % vis / 50 % doc
HAL: 6 V, 30 W
LED: White light LED
BioSciences | Jena Location
Phone : +49 3641 64 3400
Telefax: +49 3641 64 3144
E-Mail: micro@zeiss.de
www.zeiss.de/micro
60-2-0058/e – published 12.09
Carl Zeiss MicroImaging GmbH
07740 Jena, Germany
Information subject to change.
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