Microscope Components for OEM Integration

Microscope Components
Guide
Microscope Components for OEM Integration
Contents
1. Welcome to UIS2 Optics
1-1
2. System Diagram
BX53M System Diagram
(for Reflected and Combination Reflected/Transmitted Light)
2-1
BXFM System Diagram
2-3
BX63 System Diagram
2-5
2-7
BX53 System Diagram
2-9
BX43 System Diagram
2-11
BX3 Series Basic Motorized System Diagram
BX2 Series BXFM System Diagram
2-12
2-13
BX2 Series Motorized Unit System Diagram
2-13
BXFM-A System Diagram
3. UIS2 Objectives
UIS2 Objectives (for Industrial Microscopes)
3-1
3-2
M Plan Apochromat
MPLAPON series
3-2
M Plan Apochromat Oil
MPLAPON100XO
M Plan Semi Apochromat
MPLFLN series
3-3
Long Working Distance M Plan Semi Apochromat
3-4
LMPLFLN series
3-5
M Plan Achromat
MPLN series
LCD Long Working Distance M Plan Semi Apochromat
LCPLFLN-LCD series
3-6
Super Long Working Distance M Plan Achromat
3-7
SLMPLN series
IR Long Working Distance M Plan Achromat
LMPLN-IR series
3-8
3-9
IR M Plan Achromat
LCPLN-IR series
3-10
M Plan Semi Apochromat BD
MPLFLN-BD series
M Plan Semi Apochromat BDP
MPLFLN-BDP series
3-11
Long Working Distance M Plan Semi Apochromat BD
3-12
LMPLFLN-BD series
3-13
M Plan Achromat BD
MPLN-BD series
White Light Interferometry Objective
WLI100XMRTC
3-14
3-15
UIS2 Objectives (for Life Science Microscope)
Universal Plan Super Apochromat
UPLSAPO series
3-16
3-18
Plan Apochromat
PLAPON series
Universal Plan Semi Apochromat/Plan Semi Apochromat
3-19
UPLFLN, PLFLN series
Plan Achromat
PLN series
3-21
Universal Plan Semi Apochromat for Phase Contrast
3-23
UPLFLN-PH series
3-24
Plan Achromat for Phase Contrast
PLN-PH series
Universal Plan Semi Apochromat for Polarizing
UPLFLN-P series
3-25
3-26
Achromat for Polarizing
PLN-P, ACHN-P series
Plan Achromat (ND)
PLN-CY, PLFLN-CY series 3-27
Long Working Distance Universal Plan Semi Apochromat
3-28
LUCPLFLN series
Long Working Distance Universal Plan Semi Apochromat for Relief
Contrast
CPLFLN-RC, LUCPLFLN-RC series
3-29
Long Working Distance Universal Plan Semi Apochromat for Phase
Contrast
CPLFLN-PH, LUCPLFLN-PH series
3-30
Culture Specimen Objectives for Phase Contrast
3-31
CPLN-PH, LCACHN-PH series
Culture Specimen Objectives for Relief Contrast
CPLN-RC, LCACHN-RC series
3-32
No Cover Water Immersion for Fixed Stage Upright Microscope
3-33
UMPLFLN-W, LUMPLFLN-W series
No Cover Water Immersion for Fixed Stage Upright Microscope
XLUMPLFLN20XW
3-34
3-35
Universal Apochromat
UAPON 340 series
3-36
TIRF Objectives
APON, UAPON series
4. Microscope Frames
BX53M: Upright Transmitted and Reflected Light Microscope Frame
BX53MTRF-S
4-1
BX53M: Upright Reflected Light Microscope Frame
4-2
BX53MRF-S
BX3: Automated Transmitted Light Microscope Frame
BX63F
4-3
BX3: Semi-Motorized Fluorescence Transmitted Light Microscope Frame
4-4
BX53F
BX3: Manual System Transmitted Light Microscope Frame
BX43F
4-5
BX3: Transmitted Ergonomic Microscope Frame
4-6
BX46F
BX2: Upright Motorized Transmitted/Reflected Light Microscope Frame
BX61TRF
4-7
4-8
BXFM Frame
BXFM-F
BXFM System Configuration Example 1
BXFM-F + BXFM-ILH + BXFM-ILHSPU
4-9
BXFM System Configuration Example 2
4-10
BXFM-F + BXFM-ILHS
Stands for BXFM
4-11
5. Illumination Units
5-1
Reflected Light Illuminator for BX53M
5-2
Coded Reflected Light Illuminator for BX53M Frame
Reflected Light Illuminator for BX3 Series
5-3
5-4
Reflected Light Illuminator for BX2 series
Mounting Dimensions of Illuminator
(BX3M-RLA-S, BX3M-RLAS-S, BX3M-URAS-S, BX3M-KMA-S,
BX3-RFAS, BX3-URA)
Mounting Dimensions of Illuminators
5-5
(BX-RLA2 and BX-URA2)
Compact Reflected Light Illuminator for BF
5-6
6. Light Source Units
LED Lamp Housing for BX53M
6-1
6-2
LED Lamp Housing for BX3 Series
6-3
Lamp Housings
Halogen Illumination
6-4
6-5
Halogen Fiber Illumination Accessories
6-6
Lamp Housing Accessory
7. Condenser Units
Universal Condenser
7-1
7-2
Condenser
8. Observation Tubes
8-1
Super Widefield Trinocular Observation Tubes
Widefield Trinocular Observation Tubes
8-2
8-2
Single Port Tube with Lens
8-3
Tilting Binocular Tube
8-4
Binocular Tube
9. Intermediate Tubes Accessories
Intermediate Tubes
9-1
9-3
Dual Port Tube with C Mounts
10. Eyepieces
Eyepieces
10-1
11. Revolving Nosepieces
11-1
Revolving Nosepieces for BF Objectives
Revolving Nosepieces for BF/DF Objectives
11-2
11-3
Coded Sextuple Revolving Nosepiece
12.Video Camera Adaptors
12-1
C-Mount Video Camera Ports
Video Camera Mount Adaptors
12-2
12-2
Video Camera Port
13. Motorized Units
13-1
Control Box for BX53M/BXFM
Motorized Universal Reflected Illuminator for BX2 Series
13-2
13-3
Motorized Units
13-4
Control Box for BX2 Series
13-5
Motorized Units for BX2 Series
Motorized Modular Microscope
13-6
Motorized Units for BX3 Series
13-7
13-8
Control Box for BX3 Series
14-1
14. Optical Terminology
Welcome to UIS2 Optics
UIS2:
Maximize the Advantage of Infinity-Corrected Optics
What are infinity-corrected optics?
or task-specific optical systems. To establish real
flexibility with such a system, it is necessary to
eliminate coma aberration. **
The UIS2 infinity-corrected optical system is
designed so that light passes from the specimen
through the objectives without forming an image
along the way. Instead, light travels in the form of
parallel rays to the tube lens, is focused by the tube
lens, and forms an intermediate image. In finitecorrected optics, the intermediate image is formed
by the objective without a tube lens.
**In UIS2 objectives, the parfocal distance is designed at
45 mm and the focal length of the tube lens is 180 mm.
Figure 2. Advantages of Infinity-corrected
Optical System
Infinity-Corrected Optical System
Finite-Corrected Optical System
Figure 1. Infinity-Corrected and
Finite-Corrected Optical System Principles
Objective
Infinity-Corrected Optical System
Parallel Light Beam
UIS/UIS2
Objective
Tube Lens
Eyepiece
The UIS2 optical system corrects aberration with a
dedicated telan lens and eyepiece; coma aberration
and flatness are not degraded even when the telan
lens’ exit pupil position is modified by changing the
objective and telan distance. This makes it possible
to use a distance of 50 mm to 170 mm from the
objective mounting position to the single port tube
with lens.
Intermediate
Image
Advantages of Infinity-corrected Optics
Infinity-corrected
advantages:
optics
Objective
Basic Dimensions in the UIS2 Optical System
Intermediate
Image
Finite-Corrected Optical System
Objective
Tube Lens
Eyepiece
offer
a
number
of
*See definition in the optical terminology section.
• There is no change in magnification, even when
the distance between the objective and tube lens
is altered.
• Because the total magnification remains constant,
there is no image aberration — even when prisms
or sliders are interposed between the objectives
and the tube lens.
Figure 3. Basic Dimensions of UIS2 Optical System
U-TLU
(Single Port Tube with Lens)
Objective
*40 mm
Image
*84 mm
45 mm Recommended Distance
50–170 mm
The advantages of UIS2 infinity-corrected optics are
important when designing the ideal microscope
optical system. With infinity-corrected optics, users
can freely insert or remove intermediate attachments
in the parallel rays of light between the objectives
and tube lens, enabling the creation of user-specific
57.6 mm
102 mm
*Basic dimensions when our revolving nosepiece and illuminator are
combined. When the position of the illuminator is changed,
the illuminator's performance cannot be maintained.
1-1
Features of UIS2 Objectives
4. Reduced Weight
UIS2 objectives (MPLFLN and LMPLFLN series)
feature an aluminum objective barrel cover,
reducing their weight to approximately 2/3 that of
conventional objectives. This lightens the load on
devices when the objectives are moved up and
down, suppressing vibrations by lowering the
inertia generated when users switch objectives.
UIS2 objective lenses are compatible (in both screw
diameter and optical performance) with the UIS
optical system and offer the following features as
compared to conventional objectives.
1. Wavefront Aberration Control
Olympus UIS2 objectives push the boundaries of
performance with wavefront aberration control,
high numerical apertures (NA), and long working
distances. Our objectives are designed to
provide splendid performance by minimizing the
aberrations that reduce resolution.
5. Lead and Cadmium Free
UIS2 objectives are made from lead- and
cadmium-free eco-glass.
***See definition in the optical terminology section.
2. Objective Lenses with Splendid Image
Parcentricity
Semi Apochromatic UIS2 objectives have
splendid parcentricity. When a user changes
objectives by rotating the nosepiece, the center
of the field of view does not change on the digital
camera (50X magnification or higher in the
MPLFLN and LMPLFLN series objectives).
3. Improved Color Reproducibility
UIS2 objectives realize true color reproduction
without chromatic shifts using specially selected
high-transmittance glass and advanced coating
technology. These features provide high
transmittance that is flat over a wide-band
wavelength. Because the entire optical system,
including the tube lens, is designed to reproduce
a true color users know that they can obtain
realistic images of the specimen even without
using a digital microscope camera.
1-2
System Diagram
BX53M System Diagram (for Reflected and Combination Reflected/Transmitted Light
Digital cameras
Camera adaptors
U-CMAD3 + U-TV1X-2
U-TV1XC
U-TV0.63XC
Tubes and eyepieces
U-TV0.5XC-3
U-TV0.35XC-2
U-TV0.25XC
Widefield
trinocular tube
U-TR30-2
U-ETR-4
U-TTR-2
Intermediate tubes
Super widefield
trinocular tube
U-SWTR-3
U-SWETTR-5
Magnification
changer
U-CA
U-ECA
Eyepoint
adjuster
U-EPA2
Eyepieces
WHN10X
WHN10X-H
CROSS
WHN10X
Binocular
tube
U-BI30-2
U-TBI30-3
Single tube
U-TLU
Eyepieces
SWH10X-H
CROSS
SWH10X
Digital cameras
Trinocular camera adaptor
U-TRU + Camera adaptors
Extension camera adaptor
U-DP + U-DP1XC
Illumination
×2
×2
Reflected
LED light for BF
BX3M-KMA-S
Coded
reflected
LED light
for BF/DF/POL
BX3M-RLAS-S
Reflected light
for BF/DF
BX3M-RLA-S
LED light
source
BX3M-LEDR
Coded universal
reflected light
BX3M-URAS-S
Halogen
light source
U-LH100L-3 +
TH4-100/200
Option:
U-RMT/TH4-HS
Rotatable
analyzer
U-AN360-3
Polarizer slider
for reflected
light
U-PO3
Polarizer
U-POTP3
ND filters
U-25ND50
U-25ND25
U-25ND6
Mirror units
U-FF
U-FDICR
U-FBF U-FWBS
U-FDF U-FWGS
U-FBFL U-FWUS
LED light
source
BX3M-LEDR
Option:
U-RCV
Nosepieces
Analyzer for
reflected light
U-AN-2
Halogen light
source
U-LH100L-3
Option:
U-RMT/
TH4-HS
Mercury light source
U-LH100HGAPO +
U-RFL-T
U-LH100HG + U-RFL-T
Option:
U-RCV
Filters
U-25LBD
U-25LBA
U-25IF550
U-25L42
U-25Y48
U-25FR
U-25
Light guide source
U-LLGAD + U-LLG150
(U-LLG300) + U-HGLGPS
Option:
U-RCV
Objectives and sliders
For BF
U-5RE-2
U-5RES-ESD
For BF with
slider slot
U-D6RE
U-D6RE-ESD-2
U-P6RE
U-D7RE
U-D6RES
U-D7RES
U-D6REMC
For BF/DF
U-5BDRE
Frames
BF
objectives
For BF/DF
with slider slot
U-D5BDRE
U-P5BDRE
U-D6BDRE
U-D5BDREMC
U-D5BDRES-ESD
U-D6BDRES-S
U-D6BDREMC
DIC sliders
U-DICR
U-DICRH
U-DICRHC
BF/DF
objectives
BF
objectives
MIX slider
U-MIXR
DIC sliders
U-DICR
U-DICRH
U-DICRHC
BF/DF
objectives
BD-M-AD
BF
objectives
DIC sliders
U-DICR
U-DICRH
U-DICRHC
Height adaptor
BX3M-ARMAD
Controller for BX3M
BX53M frame for
reflected light
BX53MRF-S
BX53M frame for
reflected/transmitted light
BX53MTRF-S
Control box
BX3M-CB
Transmitted
LED lamp housing
BX3M-LEDT
Hand switch
Stand-alone
connection kit
U-HSEXP
BX3M-HSRE DP2-SAL
BX3M-HS
PC with OLYMPUS
Stream Software
Controller for BX3M
Stages
Rotatable
stage
U-SRG2
U-SRP
Plain stage
U-SP
Mechanical right/left
hand control stage
U-SVRM U-SVLM
Option: U-SHG/U-SHGT
Right/left hand control
large-size stage
U-SIC4R2 U-SIC4L2
Stage plate
U-MSSP4
Mechanical
stage
U-FMP
Condensers
Stage plate
U-MSSP
Specimen
holders
U-HLD-4*
U-HLDT-4*
U-HRD-4*
U-HRDT-4*
Plate
U-WHP2
Stage glass
plate
U-MSSPG*
Stage plate
U-SP64
Long working
distance condenser
U-LWCD*
Condenser
U-AC2* U-SC3*
U-POC-2*
2-1
Wafer holder plate
U-WHP64
Wafer holder plate
BH2-WHR43
BH2-WHR54
BH2-WHR65
Wafer holder plate
BH2-WHR43
Filter (Ø45)
U-POT* 43IF550-W45*
45-IF546* U-FC*
*For transmitted light combination only
Right hand control 150 mm x 100 mm stage
U-SIC64
Option: U-SHG U-SHGT
Stage glass plate
U-SPG64*
Control box and cable connection diagram
Coded nosepiece
U-5RES-ESD
U-D5BDRES-ESD
U-D6RES
U-D7RES
U-D6BDRES-S
Coded Illuminator
BX3M-RLAS-S
BX3M-URAS-S
Reflected LED
lamp housing
BX3M-LEDR
Motorized nosepiece
U-D6REMC
U-D5MDREMC
U-D6BDREMC
MIX slider for
reflected light
observation
U-MIXR
Cable for
motorized nosepiece
BX3M-RMCBL
Cable for
U-MIXR
U-MIXRCBL
PC with OLYMPUS
Stream Software
OR
Control box
BX3M-CB
Frame
BX53MRF-S
BX53MTRF-S
Stand-alone
connection kit
DP2-SAL
Transmitted LED
lamp housing
BX3M-LEDT
Hand switch
BX3M-HS
Hand switch
for motorized
nosepiece
BX3M-HSRE
Hand switch for
exposure
U-HSEXP
Stand-alone light manager configuration
Reflected light or transmitted light
Manual, coded, or motorized nosepiece
(Ergonomic hand switch configuration)
Manual nosepiece
U-P4RE
U-5RE-2
U-D6RE
U-D6RE-ESD-2
U-P6RE
U-D7RE
U-5BDRE
U-D5BDRE
U-P5BDRE
U-D6BDRE
Manual or coded Illuminator
Manual Illuminator
BX3M-KMA-S
OR
OR
BX53M frame for
reflected light
BX53MRF-S
Coded nosepiece
U-5RES-ESD
U-D7RES
U-D5BDRES-ESD U-D6BDRES-S
U-D6RES
OR
BX53M frame for
reflected/transmitted light
BX53MTRF-S
Manual Illuminator
BX3M-RLA-S
OR
OR
Transmitted LED
lamp housing
BX3M-LEDT
Reflected LED lamp housing
BX3M-LEDR
Motorized nosepiece
U-D6REMC
U-D5BDREMC
U-D6BDREMC
Cable for
motorized
nosepiece
BX3M-RMCBL
Hand switch for
motorized nosepice
BX3M-HSRE
Control box
BX3M-CB
Coded Illuminator
BX3M-RLAS-S
OR
Coded Illuminator
BX3M-URAS-S
Reflected LED lamp housing
BX3M-LEDR
MIX observation configuration
Frame, control box, and hand switch
Nosepiece and slider
Control box
BX3M-CB
Objectives
BF/DF with slider slot for
DIC/MIX nosepiece
U-D5BDRE U-D5BDRES-ESD
U-P5BDRE U-D6BDRES-S
U-D6BDRE U-D5BDREMC
U-D6BDREMC
Frame
BX53MRF-S
BX53MTRF-S
Hand switch
BX3M-HS
MIX slider for
reflected light observation
U-MIXR
PC with OLYMPUS
Stream Software
2-2
Cable for U-MIXR
U-MIXRCBL
BF/DF objectives
MPLN BD series
MPLFLN BD series
MPLFLN BDP series
LMPLFLN BD series
System Diagram
BXFM System Diagram
Camera adaptors
Digital cameras
U-CMAD3 + U-TV1X-2
U-TV1XC
U-TV0.63XC
U-TV0.5XC-3
U-TV0.35XC-2
U-TV0.25XC
Tubes and eyepieces
Widefield
trinocular tube
U-TR30-2
U-ETR-4
U-TTR-2
Super widefield
trinocular tube
U-SWTR-3
U-SWETTR-5
Eyepieces
WHN10X
WHN10X-H
CROSS
WHN10X
Binocular
tube
U-BI30-2
U-TBI30-3
Single tube
U-TLU
Eyepieces
SWH10X-H
CROSS
SWH10X
Intermediate tubes
Magnification
changer
U-CA
U-ECA
Digital cameras
Eyepoint
adjuster
U-EPA2
Extension camera adaptor
U-DP + U-DP1XC
Trinocular camera adaptor
U-TRU + Camera adaptors
Illumination
×2
×2
Reflected
LED light
for BF
BX3M-KMA-S
Coded reflected Reflected light
LED light
for BF/DF
for BF/DF/POL BX3M-RLA-S
BX3M-RLAS-S
LED light
source
BX3M-LEDR
Halogen light
source
U-LH100L-3 +
TH4-100/200
Option: U-RMT/TH4-HS
Power supply
for LED
BX3M-PSLED
LED light
source
BX3M-LEDR
Option:
U-RCV
×2
Coded universal
reflected light
BX3M-URAS-S
Reflected light
for BF
U-KMAS
Mirror units
U-FF
U-FDICR
U-FBF U-FWBS
U-FDF U-FWGS
U-FBFL U-FWUS
LED light
source
BX3M-LEDR
Halogen light
source
U-LH100L-3 +
TH4-100/200
Option:
U-RMT/
TH4-HS
Mercury light
source
U-LH100HGAPO +
U-RFL-T
U-LH100HG +
U-RFL-T
Option: U-RCV
Light guide
source
U-LLGAD +
U-LLG150
(U-LLG300) +
U-HGLGPS
Option: U-RCV
Analyzer for
reflected light
U-AN-2
Rotatable
analyzer
U-AN360-3
Polarizer slider
Polarizer
for reflected light U-POTP3
U-PO3
Halogen light
source
U-LH100L-3 +
TH4-100/200
Option:
U-RMT/TH4-HS
ND filters
U-25ND50
U-25ND25
U-25ND6
Power supply
for LED
BX3M-PSLED
Filters
U-25IF550
U-25L42
U-25Y48
U-25FR
U-25
Power supply
for LED
BX3M-PSLED
Illuminator holder for BX3M
BX3M-ILH
Option: BXFM-ILHSPU
lluminator holder BXFM
BXFM-ILHS
Nosepieces
Objectives
and sliders
For BF
U-5RE-2
U-5RES-ESD
For BF with
slider slot
U-D6RE
U-D6RE-ESD-2
U-P6RE
U-D7RE
U-D6RES
U-D7RES
U-D6REMC
For BF/DF
U-5BDRE
BF
objectives
For BF/DF
with slider slot
U-D5BDRE
U-P5BDRE
U-D6BDRE
U-D5BDREMC
U-D5BDRES-ESD
U-D6BDRES-S
U-D6BDREMC
U-DICR U-MIXR
U-DICRH
U-DICRHC
U-DICR
U-DICRH
U-DICRHC
U-DICR
U-DICRH
U-DICRHC
BD-M-AD
BF/DF
objectives
BF
objectives
BF/DF
objectives
BF
objectives
Frame
Controller for BXFM
BXFM frame
BXFM-F
BXFM control box
BX3M-CBFM
Controller for BXFM
Control box for
coded function
U-CBS
Stand
U-ST*
SZ-STL
*U-ST is not available with BX3M-ILH.
2-3
Hand switch
BX3M-HSRE
BX3M-HS
Stand-alone
connection kit
DP2-SAL
PC with OLYMPUS
Stream Software
Hand switch
for exposure
U-HSEXP
Stand-alone
connection kit
DP2-SAL
PC with OLYMPUS
Stream Software
Control box and cable connection diagram
Coded nosepiece
U-5RES-ESD
U-5BDRES-ESD
U-D6RES
U-D7RES
U-D6BDRES-S
Coded Illuminator
BX3M-RLAS-S
BX3M-URAS-S
PC with OLYMPUS
Stream Software
MIX slider for
reflected light
observation
U-MIXR
Extension cable
for motorized
nosepiece
BX3M-RMECBL
Extension cable for
U-MIXR
U-MIXRECBL
BXFM control box
BX3M-CBFM
Control box for
coded function
U-CBS
Hand switch
for exposure
U-HSEXP
Motorized nosepiece
U-D6REMC
U-D5BDREMC
U-D6BDREMC
Stand-alone
connection kit
DP2-SAL
Hand switch
BX3M-HS
Note:
DP2-SAL is available only with either the coded system or
the motorized system, but not with both.
Hand switch
for motorized
nosepiece
BX3M-HSRE
MIX observation configuration
Frame, control box, and hand switch
Nosepiece and slider
Objectives
BF/DF with slider slot for DIC/MIX
U-D5BDRE U-D5BDRES-ESD
U-P5BDRE U-D6BDRES-S
U-D6BDRE U-D5BDREMC
U-D6BDREMC
BXFM control box
BX3M-CBFM
Hand switch
BX3M-HS
MIX slider for
reflected light observation
U-MIXR
Extension cable for
U-MIXR
U-MIXRECBL
Motorized nosepiece configuration
Control box and hand switch
BXFM control box
BX3M-CBFM
Nosepiece and slider
Hand switch
for motorized
nosepiece
BX3M-HSRE
Motorized nosepiece
U-D5BDREMC
U-D6REMC
U-D6BDREMC
2-4
Extension cable
for motorized
nosepiece
BX3M-RMECBL
BF/DF objectives
MPLN BD series
MPLFLN BD series
MPLFLN BDP series
LMPLFLN BD series
System Diagram
BX63 System Diagram
TR-Adaptor
U-FMT
F-mount adaptor
U-SMAD
Sony-mount
adaptor
U-TMAD
T-mount adaptor
U-BMAD
Bayonet-mount
adaptor
WHN10X-H,
CROSS WHN10X
Eyepieces
U-CT30-2
Centering telescope
U-TVZ
Zoom
adaptor
WHN10X,
* WHN10X-H,
CROSS WHN10X
Eyepieces
U-CT30-2
Centering telescope
U-CMDPTS
C-mount adaptor
for U-DPTS
U-TV1X-2
TV adaptor
U-DPCAD
Dual port tube
with C-mounts
U-TR30NIR
Trinocular tube
U-ETR-4*1
Erect image
trinocular tube
U-CMAD3
C-mount adaptor
U-TR30-2
Trinocular tube
U-DPTS
Multi double port
tube
U-TV0.63XB
0.63X
B4-mount
adaptor
U-CMDPTS
C-mount adaptor
for U-DPTS
U-BI30-2
Binocular tube
U-TTR-2
Tilting trinocular
tube
U-TV0.25XC
0.25X
C-mount
adaptor
U-TBI-3*1
Tilting binocular tube
E
U-D7REA
Motorized 7-position nosepiece
U-GAN
Analyzer for
urate crystals
observation
A
A
U-IFCBL50
Interface cable, 50 cm
U-ANT
Analyzer for
transmitted light
A
U-D7RES
Coded 7-position
nosepiece
U-D6RES
Coded 6-position
nosepiece
U-DICT
DIC slider for
transmitted light
OBJECTIVES
U-DICTS
Shift DIC slider for transmitted light
U-DICTHR
High-resolution DIC slider
for transmitted light
U-DICTHC
High-contrast DIC slider
for transmitted light
BX3-ARM
Standard arm
E
A
U-LH100ADP
LH100 adaptor
U-AW
Motorized
attenuator wheel
U-IFCBL15
Interface cable, 15 cm
U-IFCBL200
Interface cable, 200 cm
A
B
B
U-IFCBL200
Interface cable,
200 cm
C
U-MCZ
Controller
BX63F
BX63 frame
U-POT
Polarizer
U-LH100-3
100 W halogen
lamp housing
U-LHLEDC
LED lamp
housing
F
U-RMT
Extension cord
Filter (ø45)
Touch-screen controller
(attached to BX63F)
U-FC
Filter cassette
U-HLD-4,
U-HLDT-4
Specimen holder
U-HRD-4,
U-HRDT-4
Specimen holder
U-HLS-4,
U-HLST-4
Specimen holder
XY-controller
(attached to BX3-SSU)
U-CST
Centering target
U-SHG
U-SHGT
Rubber grips
U-SVLO
Oil rectangular
stage with
left-hand
control
U-SVRO
Oil rectangular
stage with
right-hand
control
U-SVLB-4
Mechanical
stage with
left-hand
control
U-SVRB-4
Mechanical
stage with
right-hand
control
D
Control box
(attached to BX3-SSU)
BX3-SSU
Ultrasonic stage
BX3-STAD
Stage adaptor
*1 Slight vignetting may occur in combination with an additional intermediate attachment or fluorescence illuminator. *2 Requires an additional intermediate attachment or fluorescence illuminator.
*3 Cannot be used with BX3-UCD8A and U-UCD8-2
2-5
CAMERAS
U-ANT
Analyzer for
transmitted light
U-KPA
Intermediate attachment for
simple polarizing observation
U-TV0.35XC-2
0.35X
C-mount
adaptor
U-TV0.63XC
0.63X
C-mount
adaptor
U-TV0.5XC-3
0.5X
C-mount
adaptor
U-CA
Magnification changer
U-TV1XC
C-mount
adaptor 1X
(XY adjustment)
U-EPA2
Eyepoint adjuster
U-EPAL-2
Eyepoint adjuster
U-TBI-3-CLI*1
Tilting binocular
tube
U-ETBI
Ergonomic
erect image
binocular tube
U-TTBI
Ergonomic
binocular tube
U-TTLBI*2
Tilting, telescopic,
lifting binocular tube
U-LLGAD
Liquid light
guide
adaptor
BX3-RFAS
Coded fluorescence illuminator
U-LLG150/
U-LLG300
Liquid light
guide
(1.5 m/3 m)
U-HGLGPS
Light source
U-LH75XEAPO
75 W xenon
apo lamp
housing
A
U-RFL-T
E
U-LHEAD
Extension adaptor
for lamp housing
Mirror units
BX3M
B
C
D
F
A
U-IFCBL15
Interface cable, 15 cm
U-LH100HG
100 W mercury
lamp housing
U-RFL-T
LIFE TIME
U-RFL-T
Power supply unit
for mercury lamp
A
B
C1394A
Interface cable
BX3-CBH
Control box
IX2-GCP
Glass stage
insert plate
U-RX-T
Power supply unit
for xenon lamp
BURNER ON
U-IFCBL15
Interface cable, 15 cm
U-IFCBL200
Interface cable, 200 cm
U-AW
Motorized
attenuator wheel
E BX3-RFAA
Motorized
fluorescence
illuminator
U-LH100HGAPO
100 W mercury
apo lamp
housing
U-DULHA
Double lamp housing
adaptor
U-AN-2
Analyzer slider
LIFE TIME
BURNER ON
PC (software)
U-TLO
Oil top lens
IX-CP50
Insert plate
IX-SCL
Slide clip
U-SC3
Swing-out
condenser
U-AAC
Achromatic/
aplanatic
condenser
U-ULC-2
Ultra-low
condenser
U-DCD
Darkfield
condenser, dry
U-TLD
Dry top lens
U-DCW
Darkfield
condenser, oil
U-UCD8-2
8-position
universal
condenser
IX-SVL2*3
Cross stage with short
left handle
Optical elements
BX3-UCD8A
Motorized
universal
condenser
A
U-IFCBL15
Interface cable, 15 cm
U-CO1.25X
Low magnification
conversion lens for UCD
2-6
System Diagram
BX53 System Diagram
TR-Adaptor
WHN10X-H,
CROSS WHN10X
Eyepieces
U-CT30-2
Centering telescope
U-FMT
F-mount adaptor
U-TMAD
T-mount adaptor
U-CMAD3
C-mount adaptor
U-BMAD
Bayonet-mount
adaptor
U-SMAD
Sony-mount
adaptor
WHN10X-H,
* WHN10X,
CROSS WHN10X
Eyepieces
U-CT30-2
Centering telescope
U-TVZ
Zoom
adaptor
SWH10X-H,
CROSS SWH10X,
MICRO SWH10X
Eyepieces
U-CT30-2
Centering telescope
U-SWETTR-5
Super widefield
erect image
tilting trinocular
observation tube
U-TV1X-2
TV adaptor
U-ETR-4*1
Erect image
trinocular tube
U-SWTR-3
Super widefield
trinocular tube
*
U-GAN
Analyzer for urate crystals
observation
U-ANT
Analyzer for
transmitted light
U-DICT
DIC slider for
transmitted light
U-CMDPTS
C-mount adaptor
for U-DPTS
U-DPCAD
Dual port tube
with C mounts
U-TR30NIR
Trinocular tube
*
U-TTR-2
Tilting trinocular tube
*
A
B
B
U-D7RES
Coded 7-position
nosepiece
U-D6RES
Coded 6-position
nosepiece
C
C
BX53F
BX53 frame
U-D6RE
Sextuple revolving
nosepiece for
DIC/simple POL
A
BX3-RFAA*4
Motorized fluorescence
illuminator
U-AN-2
Analyzer slider
Mirror units
BX3-RFAS*4
Coded fluorescence
illuminator
U-ANT
Analyzer for
transmitted light
C
B
U-AN-2
Analyzer slider
U-LH100-3
100 W halogen
lamp housing
U-POT
Polarizer
U-TAD
Plate adaptor
U-FC
Filter cassette
BX3-URA
Universal reflected illuminator
Filter (ø45)
C
U-AN-2
Analyzer slider
COMPENSATORS
U-HLD-4,
U-HLDT-4
Specimen
holder
U-CST
Centering
target
U-FMP
Mechanical
stage
U-SRG2
Rotatable
graduated
stage
*
U-D7RE
Septuple revolving
nosepiece for
DIC/simple POL
BX3-ARM
Standard arm
U-DICTS
Shift DIC slider for
transmitted light
U-DICTHR
High-resolution DIC
slider for transmitted light
U-DICTHC
High-contrast DIC slider
for transmitted light
U-TBI-3*1
Tilting binocular tube
U-BI30-2
Binocular tube
*
U-D7REA
Motorized
7-position
nosepiece
OBJECTIVES
U-CMDPTS
C-mount adaptor
for U-DPTS
U-DPTS
Multi double port
tube
U-TR30-2
Trinocular tube
U-TV0.63XB
0.63X
B4-mount
adaptor
U-SRP
Precision
rotatable
stage
U-HRD-4,
U-HRDT-4
Specimen
holder
U-HLS-4,
U-HLST-4
Specimen
holder
U-SHG
Rubber grip
U-SHGT
Rubber grip
U-SP
Plain
stage
U-SVRO
Oil rectangular
stage with
right-hand
control
U-SVLO
Oil rectangular
stage with
left-hand
control
U-SVRB-4
Mechanical
stages with
right-hand
control
U-SVLB-4
Mechanical
stages with
left-hand
control
*1 Slight vignetting may occur in combination with an additional intermediate attachment or observation method. *2 Requires an additional intermediate attachment or fluorescence illuminator.
*3 Cannot be used with U-TTLBI. *4 Compatible with FN 22. *5 Cannot be used with BX3-URA. *6 Stand is standard equipment with the U-MDOSV and U-MDO10R3. *7 An auxiliary lens is
equipped.
2-7
BX3-25ND6
ND filter
BX3-25ND25
ND filter
CAMERAS
U-ECA
Magnification changer 2X
U-ANT
Analyzer for
transmitted light
U-KPA
Intermediate attachment for
simple polarizing observation
U-CA
Magnification changer
U-TV0.25XC
0.25X
C-mount
adaptor
U-TV0.35XC-2
0.35X
C-mount
adaptor
U-TV0.5XC-3
0.5X
C-mount
adaptor
U-TV1XC
C-mount
adaptor 1X
(XY adjustment)
U-TV0.63XC
0.63X
C-mount
adaptor
U-DP1XC
Dual port 1X
U-DP*1*3
Dual port
U-APT
Arrow pointer
U-TBI-3-CLI*1
Tilting binocular tube
*
U-EPA2
Eyepoint adjuster
*
*
U-EPAL-2
Eyepoint adjuster
U-TTBI
Ergonomic
binocular tube
U-TTLBI*2
Tilting,
telescopic,
lifting binocular
tube
U-ETBI
Ergonomic erect
image binocular
tube
U-TRU*1*3
Trinocular intermediate unit
U-TRUS*1
C
U-P4RE
Centerable
revolving
nosepiece
U-P6RE
Centerable sextuple
revolving nosepiece
Trinocular intermediate unit
U-CPA
Intermediate attachment for
conoscopic and orthoscopic
observation
U-5RE-2
Quintuple
revolving
nosepiece
U-AN360P-2
Rotatable analyzer
U-OPA
Intermediate attachment for
orthoscopic observation
U-LLGAD
Liquid light
guide
adaptor
U-LHEAD*5
Extension
adaptor for
lamp housing
U-LLG150/
U-LLG300
Liquid light
guide
(1.5 m/3 m)
U-LH75XEAPO
75 W xenon
apo lamp
housing
U-DULHA
Double
lamp house
adaptor
U-LH100HGAPO
100 W mercury
apo lamp
housing
U-DAL10X
Drawing attachment (10X)
U-DA
Drawing attachment
U-HGLGPS
Light source
U-DO3
Dual observation attachment
U-SDO3
Side-by-side observation
attachment
LIFE TIME
BURNER ON
U-RX-T
Power supply unit
for xenon lamp
LIFE TIME
U-MDOB3
Multi-observation
body
BURNER ON
A
U-LH100HG
100 W mercury
lamp housing
U-AW
Motorized
attenuator wheel
U-MDOSV*6
Multi-observation
side viewer
U-RFL-T
Power supply unit
for mercury lamp
U-MDO10B3
Multi-observation body
for 10 persons
U-MDO10R3*6
Multi-observation body for 10 persons
U-AC2
U-LC*7
Abbe
Lowmagnification condenser
condenser
U-SC3
Swing-out
condenser
U-AAC
U-ULC-2
Achromatic/ Ultra-low
aplanatic
condenser
condenser
U-PCD2
U-POC-2
Phase/darkfield Polarizing
condenser
condenser
U-HSEXP
Hand switch for
exposure
Stand*6
U-CBS
Control box for
coded function
Optical devices
U-CO1.25X
Low-magnification
conversion
lens for UCD
A
BX3-UCD8A
Motorized universal
condenser
U-TLO
Oil top lens
U-TLD
Dry top lens
U-HSCBM
Hand switch for CBM
U-DCD
Darkfield
condenser,
dry
U-DCW
Darkfield
condenser,
oil
B
DP2-SAL
Stand-alone Connection Kit
A
U-IFCBL200
Interface cable,
200 cm
U-UCD8-2
8-position
universal condenser
U-CBM
Control box
PC (Software)
2-8
System Diagram
BX43 System Diagram
TR-Adaptor
U-FMT
F-mount
adaptor
WHN10X-H,
CROSS WHN10X
Eyepieces
U-CT30-2
Centering telescope
U-SMAD
Sony-mount
adaptor
U-TMAD
T-mount
adaptor
U-BMAD
Bayonet-mount
adaptor
U-CMAD3
C-mount
adaptor
WHN10X-H,
* WHN10X,
CROSS WHN10X
Eyepieces
U-CT30-2
Centering telescope
U-TVZ
Zoom
adaptor
SWH10X-H,
CROSS SWH10X,
MICRO SWH10X
Eyepieces
U-CT30-2
Centering telescope
U-CMDPTS
C-mount adaptor
for U-DPTS
U-TV1X-2
TV
adaptor
U-DPCAD
Dual port tube
with C-mounts
U-DPTS
Multi double
port tube
U-TV0.63XB
B4-mount
adaptor
U-CMDPTS
C-mount adaptor
for U-DPTS
U-SWETTR-5
Super widefield
erect image
tilting trinocular
observation tube
U-ETR-4*1
Erect image
trinocular tube
U-SWTR-3
Super widefield
trinocular tube
*
A
U-D7RES
Coded
7-position
nosepiece
U-GAN
Analyzer for urate
crystals observation
U-TR30NIR
Trinocular tube
U-TR30-2
Trinocular tube
*
*
U-TTR-2
Tilting trinocular tube
U-BI30-2
Binocular tube
U-TBI-3*1
Tilting binocular tube
*
*
A
U-D7RE
Septuple revolving
nosepiece for
DIC/simple POL
U-D6RES
Coded
6-position
nosepiece
U-P6RE
Centerable
revolving
nosepiece
U-D6RE
Sextuple revolving
nosepiece for
DIC/simple POL
U-P4RE
Centerable
revolving
nosepiece
U-ANT
Analyzer for
transmitted light
U-DICT
DIC slider for
transmitted light
A
OBJECTIVES
BX43F
BX43 frame
B
U-LS30-5
Lamp socket for
30 W halogen
U-DICTS
Shift DIC slider for
transmitted light
U-DICTHR
High-resolution DIC
slider for transmitted light
U-DICTHC
High-contrast DIC slider
for transmitted light
U-LS30ADP
LS30 adaptor
U-LHLEDC
LED lamp housing
TL4
Power supply
unit
45LBDIF
U-ANT
Analyzer for
transmitted light
U-POT
Polarizer
Filter (ø45)
U-TAD
Plate adaptor
U-FC
Filter cassette
COMPENSATORS
U-FMP
Mechanical
stage
U-SRG2
Rotatable
graduated
stage
U-SRP
Precision
rotatable
stage
U-HRD-4,
U-HRDT-4
Specimen
holder
U-HLD-4,
U-HLDT-4
Specimen
holder
U-CST
Centering target
U-HLS-4,
U-HLST-4
Specimen
holder
U-SHG
Rubber grip
U-SHGT
Rubber grip
U-SP
Plain
stage
U-SVRO
Oil rectangular
stage with
right-hand
control
U-SVLO
Oil rectangular
stage with
left-hand
control
U-SVRB-4
Mechanical
stages with
right-hand
control
U-SVLB-4
Mechanical
stages with
left-hand
control
*1 Slight vignetting may occur in combination with an additional intermediate attachment or observation method. *2 Requires an additional intermediate attachment or fluorescence illuminator.
*3 Cannot be used with U-TTLBI. *4 Compatible with FN 22. *5 An auxiliary lens is equipped.
2-9
CAMERAS
U-ECA
Magnification changer 2x
U-CA
Magnification changer
U-TV1XC
C-mount
adaptor 1X
(XY adjustment)
U-TV0.63XC-3
0.63X
C-mount
adaptor
U-TV0.5XC-3
0.5X
C-mount
adaptor
U-TV0.35XC-2
0.35X
C-mount
adaptor
U-TV0.25XC
0.25X
C-mount
adaptor
U-DP*1*3
Dual port
U-DP1XC
Dual port 1x
U-APT
Arrow pointer
U-TBI-3-CLI*1
Tilting binocular tube
U-TRU*1*3
Trinocular intermediate unit
*
*
*
U-TTBI
Ergonomic
binocular tube
U-TTLBI*2
Tilting,
telescopic,
lifting binocular tube
U-ETBI
Ergonomic erect
image binocular
tube
U-TRUS*3
Trinocular intermediate unit
U-ANT
Analyzer for
transmitted light
C
A
U-5RE-2
Quintuple
revolving
nosepiece
BX43-5RES
Coded
5-position
nosepiece
for BX43
U-LLGAD
Liquid light
guide
adaptor
U-KPA
Intermediate attachment for
simple polarizing observation
U-LLG150/
U-LLG300
Liquid light
guide
(1.5 m/3 m)
U-EPAL-2
Eyepoint adjuster
U-LH75XEAPO
75 W xenon
apo lamp
housing
BX3-URA
Universal reflected illuminator
U-PO
Polarizer
Mirror units
C
U-AN-2
Analyzer slider
D
BX3-6ND6
ND filter
BX3-25ND25
ND filter
BX3-RFAS*4
Coded
fluorescence
illuminator
U-RFL-T
LIFE TIME
BURNER ON
U-LH100HGAPO
100 W mercury
apo lamp
housing
C
U-EPA2
Eyepoint adjuster
U-HGLGPS
Light source
U-RX-T
Power supply unit
for xenon lamp
U-RFL-T
U-CPA
Intermediate
attachment for
conoscopic and
orthoscopic
observation
U-AN360P-2
Rotatable
analyzer
LIFE TIME
BURNER ON
U-OPA
Intermediate attachment for
orthoscopic observation
U-LH100HG
100 W mercury
lamp housing
U-RFL-T
Power supply unit
for mercury lamp
U-DAL10X
Drawing attachment 10 x
U-DA
Drawing attachment
U-DULHA
Double
lamp house
adaptor
U-DO3
Dual observation attachment
U-SDO3
Side-by-side observation
attachment
U-AC2
U-LC*5
Abbe
Low
magnification condenser
condenser
U-SC3
Swing-out
condenser
U-AAC
Achromatic/
Aplanatic
condenser
U-ULC-2
Ultra-low
condenser
U-POC-2
U-PCD2
Phase/darkfield Polarizing
condenser
condenser
U-DCD
Darkfield
condenser,
dry
U-TLO
Oil top lens
U-DCW
Darkfield
condenser,
oil
U-RX-T
Power supply unit
for xenon lamp
A
A
Optical devices
U-CO1.25X
Low magnification
conversion
lens for UCD
U-UCD8-2
8-position
universal
condenser
D
U-TLD
Dry top lens
B D
U-IFRES
Interface for
coded nosepiece
2-10
DP2-SAL
Stand-alone connection kit
U-CBS
Control box for
coded function
U-HSEXP
Hand switch for
exposure
PC (Software)
System Diagram
BX3 Series Basic Motorized System Diagram
Full Motorized System Basic Configuration for BX3 series
U-IFCBL15
Interface cable, 15 cm
BX3-RFAA
BX3-RFAS
Motorized fluorescence
illuminator
U-D7REA
U-D7RES
U-D6RES
Motorized 7-position
nosepiece
U-IFCBL50
Interface cable, 50 cm
BX3-CBH
Control box
BX3-UCD8A
Motorized universal condenser
C1394A
Interface cable
PC (software)
BX63F
BX63 frame
U-IFCBL200
Interface cable,
200 cm
U-MCZ
Controller
Touch-panel controller
(attached to BX63F)
Semi-Motorized System Basic Configuration for BX3 series
BX3-RFAA
Motorized fluorescence
illuminator
U-D7REA
Motorized 7-position
nosepiece
U-IFCBL15
Interface cable, 15 cm
U-IFCBL50
Interface cable, 50 cm
BX3-UCD8A
Motorized universal condenser
BX43F
BX43 frame
BX53F
BX53 frame
U-HSCBM
Hand switch for CBM
U-IFCBL200
Interface cable,
200 cm
U-CBM
Control box
Coded System Basic Congratulation for BX3 series
BX3-RFAS
Coded fluorescence
illuminator
U-D7RES
Coded 7-position
nosepiece
BX43F
BX43 frame
BX53F
BX53 frame
*Please refer to "Section 13 Motorized Unit" for each motorized unit in the detail.
**Please consult your nearest Olympus representative for details about motorized system configurations and combinations.
2-11
U-CBS
Control box for
coded function
PC (software)
PC (software)
BX2 Series BXFM System Diagram
Video System
(refer to pages 12-1 – 12-2)
Video camera
Video camera
C-mount
B-mount 2/3"
Video camera
S-mount 2/3"
Video camera
F-mount
U-FMT
U-CMAD3
U-BMAD
U-SMAD
U-TMAD
U-TV0.25XC
U-TV0.35XC-2
U-TV0.5XC-3
SN
U-TV0.63XC
3G00707
TOKYO, JAPAN
U-TV1X-2
U-TV1XC
Observation/Single Tubes and Eyepieces
(refer to pages 8-1 – 8-4)
WHN
Eyepieces
SWH
eyepieces
Widefield trinocular
observation tubes
Super widefield trinocular
observation tubes
U-TLU
U-TLUIR
Illumination Systems and Power Supply
(refer to pages 5-1 – 6/6)
U-AN360-3
U-AN360IR
U-POTP3
U-PO3
U-POIR
U-AN
U-25ND6, U-25ND25,
U-25LBD, U-25IF550,
U-25L42, U-25FR,
U-BP1100IR, U-BP1200IR
Revolving nosepiece
(refer to pages 11-1 – 11-3)
FS
FS
AS
5
AS
6
SHUTTER
ND
BX-RLA2
BX-URA2
U-RCV
U-KMAS
U-LGAD
Focusing Units
(refer to pages
LG-SF
LG-PS2
U-DULHA
4-8 – 4-11)
U-LH100-3
U-LH100L-3
U-LH100IR
BXFM-ILHS
BXFM-ILH
BXFM-ILHSPU
U-LH100HGAPO
U-LH100HG
U-LH75XEAPO
BXFM-F
Stands
(refer to page 4-11)
SZ-STL
U-ST
SZ2-STU2
*Different types may be offered in each area.
2-12
TH4-100
TH4-HS
U-RFL-T
U-RX-T
System Diagram
BX2 Series Motorized Unit System Diagram
(refer to pages 11-1 – 11-8)
Observation tubes
(refer to pages 8-1 – 8-4)
U-AN
(refer to pages 6-1 – 6-6)
U-AN
MX-AFDIC
U-AN360-3
Intermediate tubes
(refer to pages 9-1 – 9-3)
U-PO3
U-AFA2M-VIS
U-LH100HGAPO
U-LH100HG
U-LH75XEAPO
U-RFL-T
U-RX-T
U-POTP3
Mirror Units
U-25ND6
U-25ND25
U-25LBD
U-25IF550
U-25L42
U-25FR
U-FWR
BX-RLAA
BX-RFAA
U-LH100-3
U-LH100L-3
TH4-100
TH4-HS
U-ZPCB
Z-Board
U-DICR
U-D6REMC
(refer to page 11-2)
(refer to pages 3-1 – 3-36)
BX-UCB*
U-DICRH
U-HSTR2
BF Objectives
BD-M-AD
U-D5BDREMC
U-D6BDREMC
U-DICRHC
PC
See manual
BF/DF Objectives
OLYMPUS Stream
Control Software
*BX-REMCB is also available for BX-RLAA + motorized revolving nosepiece control (refer to page 13-3).
**These illuminators can only attach to the MX51 or BX61 frames.
BXFM-A System Diagram
(refer to page 13-1 – 13-8)
(refer to page 6-3)
ø32 Filter
Video system
(refer to pages 12-1 – 12-2)
Auxiliary lens
(provided with the BXFMA-F)
Observation tubes
(refer to pages 8-1 – 8-4)
MX-AFDIC
U-RFL-T
U-RX-T
U-LH100HG
U-LH75XEAPO
U-AN
(refer to page 6-5)
U-AFA2M-VIS
LG-SF
LG-PS2
U-LGAD
(refer to page 6-4)
BXFMA-F
U-FWR
U-LH100-3
U-LH100L-3
U-RMT
BX-UCB
U-ZPCB
Z-Board
See manual
U-D6REMC
U-P5REMC
U-D5BDREMC
U-D6BDREMC
U-P6BDREMC
Objectives
U-HSTR2
U-IFFH
PC
U-FH
2-13
OLYMPUS Stream
Control Software
Objectives (for Industrial Applications)
■ Definition for Objective Abbreviations
M PL(Plan) F L N - 1 0 0
M:
LM:
Metallurgical (no cover)
Long Working Distance
Metallurgical Use
SLM: Super Long Working
Distance Metallurgical Use
LC: Observation Through
Substrate
PL: Plan:
Corrects Field
Curvature on
the Periphery of
the Image Plane
B D
None: Achromat:
Corrects Chromatic Aberration at 2 Wavelengths of Blue
and Red
FL:
Semi Apochromat:
Corrects Chromatic Aberration in the Visible Range
(violet to red)
APO: Apochromat:
Optimally Corrects Chromatic Aberration in the Entire
Visible Band (violet to red)
Number:
Objective
Magnification
None: Brightfield
BD: Brightfield/Darkfield
BDP: Brightfield/Darkfield/
Polarizing
IR:
Infrared
LCD: Obervation Through
LCD Panels
■ Objective Notation
Magnification
Objectives Series Abbreviation (PL: Plan)
Infinity-Corrected Optical System
NA (Numerical Aperture)
Cover Glass Thickness (no cover)
For Brightfield Observation
FN (Field Number)
■ Objective Series List
Series
MPLAPON
MPLAPON O
MPLFLN
LMPLFLN
MPLN
LCPLFLN-LCD
SLMPLN
LMPLN-IR
LCPLN-IR
MPLFLN-BD
MPLFLN-BDP
LMPLFLN-BD
MPLN-BD
WLI100XMRTC
Magnification
50/100
100
1.25/2.5
5/10/20/40*2/50/100
5/10/20/50/100
5/10/20/50/100
20/50/100
20/50/100
5/10
20/50/100
5/10/20/50/100/150
5/10/20/50/100
5/10/20/50/100
5/10/20/50/100
100X
BF
DF
DIC*1
U
POL
FL
U
L
*3
L
U
U
L
*3
*3
FN
26.5
26.5
1.25X: 22/2.5X: 26.5
26.5
26.5
22
26.5
26.5
22
22
26.5
26.5
26.5
22
22
Remarks
We recommend using a polarizer and analyzer
For LCD
For near-IR observation
For near-IR observation
Mirau objective
*1 DIC Prism U-DICR: UM/LM Position, U-DICRHC: LM Position Fixed, U-DICRH: UM Position Fixed. *2 40X: BF Only *3 5–20X: U Excitation Also Possible.
: Responds; : Optimally Responds; BF: Brightfield; DF: Darkfield; DIC: Differential Interference Contrast; POL: Polarized light; FL: Fluorescence
■ Features of Each Objective Series
MPLAPON series: M Plan Apochromat — p. 3-2
This series of Plan Apochromat objectives corrects chromatic aberrations at optimal levels.
Olympus guarantees* the optical performance (correction for wavefront aberration) with a
Strehl ratio** of 95% or better. These objectives can be used with Olympus’ U-AFA2M active
auto focus unit.
LCPLN-IR series: IR Long Working Distance M Plan Achromat — p. 3-9
This series is designed for near-infrared microscopy, which is typically used to view the
internal structure of silicon wafers. These objectives have a correction collar to correct for
aberrations based on the thickness of the silicon or glass substrate.
MPLFLN-BD series: M Plan Semi Apochromat BD — p. 3-10
This series of Plan Semi Apochromat objectives provides high-level correction for chromatic
aberration with a minimum working distance of 1 mm. Since the exit pupil position of the
5X–150X objectives is standardized, the position of the DIC prism does not have to be
switched when changing the magnification.
MPLFLN-BDP series: M Plan Semi Apochromat BDP — p. 3-11
This series of Plan Semi Apochromat objectives provides high-level correction for chromatic
aberrations with a minimum working distance of 1 mm. Since the exit pupil position of the
5X–100X objectives is standardized, the position of the DIC prism does not have to be
switched when changing the magnification. This series is optimized for brightfield, darkfield,
and polarized light observations and can be used with differential interference contrast.
LMPLFLN-BD series: Long Working Distance M Plan Semi Apochromat BD — p. 3-12
This series of long working distance Plan Semi Apochromat objectives provides high-level
correction for chromatic aberration and are suitable for observing samples with height or
varying topography. Since the exit pupil position of the 5X–100X objectives is standardized,
the position of the DIC prism does not have to be switched when changing the magnification.
MPLN-BD series: M Plan Achromat BD — p. 3-13
This series of Plan Achromat objectives provides excellent image flatness up to FN 22.
WLI100XMRTC series: White Light Interferometry Objective — p. 3-14
This objective is designed to be used with Mirau-style white light interferometers and tolerates
high temperatures. The objective has a working distance of 0.7 mm and an optimized NA of
0.8 that provides improved light gathering.
*Measurement guarantee assessed with an Olympus interferometer for transmitted
wavefront measurement under the following conditions: a temperature of 23 °C + 1 °C;
measurements made within the 97% range of the pupil diameter.
**Strehl ratio: Indicates in percent (%) the ratio of the proportion of light that an actual optical
system can concentrate with respect to the proportion of light concentrated in the image
plane (central intensity) by an ideal, aberration-free optical system, with the latter serving
as 100%. A higher percentage indicates a higher quality optical system.
***Specified oil: IMMOIL-F30CC
MPLAPON100XO: M Plan Apochromat — p. 3-2
This Plan Apochromat objective is designed for oil immersion*** and features a numerical
aperture of 1.4. The objective provides excellent chromatic aberration correction and high
resolving power.
MPLFLN series: M Plan Semi Apochromat — p. 3-3
This series of Plan Semi Apochromat objectives delivers high-level correction for chromatic
aberration. The eight objectives in this series offer magnifications ranging from 1.25X to 100X
and a minimum working distance of 1 mm (except 40X). Since the exit pupil position of the
5X–100X objectives is standardized, the position of the DIC prism does not have to be
switched when changing the magnification (40X is not applicable to DIC observation). For very
low magnifications (1.25X, 2.5X), use the objectives with an analyzer, polarizer, and reflected
light illuminator.
LMPLFLN series: Long Working Distance M Plan Semi Apochromat — p. 3-4
This series of long working distance Plan Semi Apochromat objectives delivers high-level
correction for chromatic aberration. Because of the long working distance, these objectives
are suitable for observing larger samples. Since the exit pupil position of the 5X–100X
objectives is standardized, the position of the DIC prism does not have to be switched when
changing the magnification.
MPLN series: M Plan Achromat — p. 3-5
Plan Achromat objectives provide excellent image flatness up to FN 22.
LCPLFLN-LCD series: LCD Long Working Distance M Plan Semi Apochromat — p. 3-6
These objectives are designed for making observations through LCD panels and other
samples that have a glass substrate. The correction collar provides aberration correction that
can be matched to the thickness of the glass.
SLMPLN series: Super Long Working Distance M Plan Achromat — p. 3-7
These are high-magnification Plan Achromat objectives with a super long working distance.
Three magnifications, 20X, 50X, and 100X, are available. For 5X or 10X objectives, select
from the LMPLFLN series.
LMPLN-IR series: IR Long Working Distance M Plan Achromat — p. 3-8
This series is designed for near-infrared microscopy, which is typically used to view the
internal structure of silicon wafers.
3-1
Objectives
M Plan Apochromat
MPLAPON series
This series of Plan Apochromat objectives corrects chromatic aberrations at optimal levels. Olympus guarantees* the optical performance
(correction for wavefront aberration) with a Strehl ratio** of 95% or better. These objectives can be used with Olympus’ U-AFA2M active
auto focus unit.
MPLAPON50X
MPLAPON100X
ø20.32
38.43
42.4
42.78
45
(44.65)
41
37.98
41.62
45
(44.65)
4.5
4.5
ø20.32
ø5.5
W.D.=0.35
W.D.=0.35
ø8.8
ø18.9
ø20.3
ø16
* Measurement guarantee assessed with an Olympus
interferometer for transmitted wavefront
measurement under the following conditions: a
temperature of 23 °C + 1 °C; measurements made
within the 97% range of the pupil diameter.
** Strehl ratio: Indicates in percent (%) the ratio of the
proportion of light that an actual optical system can
concentrate with respect to the proportion of light
concentrated in the image plane (central intensity)
by an ideal, aberration-free optical system, with the
latter serving as 100%. A higher percentage
indicates a higher quality optical system.
ø20.2
ø28.5
ø28
Unit: mm
UIS2 Objectives
Objective
(magnification)
MPLAPON50X
MPLAPON100X
W.D.
(mm)
0.35
0.35
NA
0.95
0.95
Focal Distance
f (mm)
3.6
1.8
Weight
(g)
139
125
Widefield Eyepiece
Super Widefield Eyepiece
WHN10X FN 22
SWH10X FN 26.5
Total
Practical Field Depth of
Total
Practical Field Depth of
Magnification of View (mm) Focus (μm) Magnification of View (mm) Focus (μm)
500
0.44
1
500
0.53
1
1000
0.22
0.67
1000
0.27
0.7
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
M Plan Apochromat
MPLAPON100XO
This Plan Apochromat objective is designed for oil immersion*** and features a numerical aperture of 1.4. The objective provides
excellent chromatic aberration correction and high resolving power.
MPLAPON100XO
36.909
41.676
43.496
45
(44.9)
4.5
ø20.32
*** Specified Oil: IMMOIL-F30CC
W.D.=0.1
ø6.6
ø17.5
ø22
ø30
Unit: mm
UIS2 Objectives
Objective
(magnification)
MPLAPON100XO
NA
1.4
W.D.
(mm)
0.1
Focal Distance
f (mm)
1.8
Weight
(g)
158
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
3-2
Widefield Eyepiece
Super Widefield Eyepiece
WHN10X FN 22
SWH10X FN 26.5
Total
Practical Field Depth of
Total
Practical Field Depth of
Magnification of View (mm) Focus (μm) Magnification of View (mm) Focus (μm)
1000
0.22
0.59
1000
0.27
0.59
Objectives
M Plan Semi Apochromat
MPLFLN series
This series of Plan Semi Apochromat objectives delivers high-level correction for chromatic aberration. The eight objectives in this series
offer magnifications ranging from 1.25X to 100X and a minimum working distance of 1 mm (except 40X). Since the exit pupil position
of the 5X–100X objectives is standardized, the position of the DIC prism does not have to be switched when changing the magnification
(40X is not applicable to DIC observation). For very low magnifications (1.25X, 2.5X), use the objectives with an analyzer, polarizer, and
reflected light illuminator.
MPLFLN2.5X
ø26
MPLFLN50X
MPLFLN100X
ø20.32
ø16
ø25.8
ø15.2
ø17.8
ø26
38.4
42.4
ø7.8
W.D.=1
ø14.4
42.61
45
(44)
36.8
40.8
ø10.7
W.D.=1
ø8.5
41.78
4.8
4.8
45
(44)
35.6
40.5
41.4
45
W.D.=0.63
(44.37)
35.1
ø26
ø20.32
4.5
ø20.32
ø26
28.41
(34)
ø30
ø14.5
W.D.=11
ø29
MPLFLN40X
4.5
(41.9)
W.D.=3.1
45
45
ø20.9
W.D.=20
ø30
ø12.1
ø20.32
4.5
(25)
31.6
ø24.5
W.D.=10.7
ø29
ø20.32
45
ø20.32
45
41.2
(34.3)
4.9
(41.5)
45
W.D.=3.5
ø28.6
MPLFLN20X
MPLFLN10X
4.5
ø28
ø20.32
4.9
ø28
ø20.32
MPLFLN5X
22.55
MPLFLN1.25X
ø15.2
ø17.8
ø26
Unit: mm
UIS2 Objectives
Objective
(magnification)
MPLFLN1.25X
MPLFLN2.5X
MPLFLN5X
MPLFLN10X
MPLFLN20X
MPLFLN40X
MPLFLN50X
MPLFLN100X
NA
0.04
0.08
0.15
0.3
0.45
0.75
0.8
0.9
W.D.
(mm)
3.5
10.7
20
11
3.1
0.63
1
1
Focal Distance
f (mm)
145
72
36
18
9
4.5
3.6
1.8
Weight
(g)
122
106
51.5
68.1
70.4
120
89.9
90.9
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
3-3
Widefield Eyepiece
Super Widefield Eyepiece
WHN10X FN 22
SWH10X FN 26.5
Total
Practical Field Depth of
Total
Practical Field Depth of
Magnification of View (mm) Focus (μm) Magnification of View (mm) Focus (μm)
12.5
17.6
870
—
—
—
25
8.8
220
25
10.6
220
50
4.4
59
50
5.3
59
100
2.2
15
100
2.7
15
200
1.1
5.2
200
1.3
5.1
400
0.55
1.66
400
0.66
1.66
500
0.44
1.3
500
0.53
1.3
1000
0.22
0.73
1000
0.27
0.73
Objectives
Long Working Distance M Plan Semi Apochromat
LMPLFLN series
This series of long working distance Plan Semi Apochromat objectives delivers high-level correction for chromatic aberration. Because
of the long working distance, these objectives are suitable for observing larger samples. Since the exit pupil position of the 5X–100X
objectives is standardized, the position of the DIC prism does not have to be switched when changing the magnification.
LMPLFLN5X
LMPLFLN10X
ø20.32
ø17
LMPLFLN50X
ø22.4
ø26
W.D.=12
ø15.2
W.D.=21
W.D.=22.5
ø25.4
ø26
29.31
45
45
45
(33)
22.1
(24)
22.2
ø20.32
4.8
4.5
ø20.32
4.9
(22.5)
LMPLFLN20X
ø26
LMPLFLN100X
ø20.32
37.26
41.1
45
(41.6)
30.4
ø12.5
ø18.2
W.D.=3.4
W.D.=10.6
45
(34.4)
4.9
4.7
ø20.32
ø26
ø15
ø18.1
ø26
Unit: mm
UIS2 Objectives
Objective
(magnification)
LMPLFLN5X
LMPLFLN10X
LMPLFLN20X
LMPLFLN50X
LMPLFLN100X
NA
0.13
0.25
0.4
0.5
0.8
W.D.
(mm)
22.5
21
12
10.6
3.4
Focal Distance
f (mm)
36
18
9
3.6
1.8
Weight
(g)
50
54
73
77
94
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
3-4
Widefield Eyepiece
Super Widefield Eyepiece
WHN10X FN 22
SWH10X FN 26.5
Total
Practical Field Depth of
Total
Practical Field Depth of
Magnification of View (mm) Focus (μm) Magnification of View (mm) Focus (μm)
50
4.4
70
50
5.3
70
100
2.2
18
100
2.7
18
200
1.1
6.1
200
1.3
6.1
500
0.44
2.5
500
0.53
2.5
1000
0.22
0.87
1000
0.27
0.87
Objectives
M Plan Achromat
MPLN series
Plan Achromat objectives provide excellent image flatness up to FN 22.
MPLN5X
MPLN10X
ø20.32
37.2
41.3
42.4
45
W.D.=1.3
ø24
(43.7)
28.8
33.6
32.71
4.5
W.D.=10.6
W.D.=20
(34.4)
45
23.4
4.5
(25)
45
ø10.5
ø21
ø12
MPLN50X
ø6
ø11.9
ø16
ø15.8
ø24
ø24
MPLN100X
ø20.32
42.8
43.16
45
38.7
ø4.4
W.D.=0.21
ø6
(44.79)
37.2
41.3
42.62
W.D.=0.38
(44.62)
4.5
4.5
ø20.32
45
ø20.32
4.5
ø20.32
MPLN20X
ø11.9
ø15.8
ø11.6
ø15.6
ø24
ø24
Unit: mm
UIS2 Objectives
Objective
(magnification)
MPLN5X
MPLN10X
MPLN20X
MPLN50X
MPLN100X
NA
0.1
0.25
0.4
0.75
0.9
W.D.
(mm)
20
10.6
1.3
0.38
0.21
Focal Distance
f (mm)
36
18
9
3.6
1.8
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
3-5
Weight
(g)
64
80
111
13
116
Total Magnification
50
100
200
500
1000
Widefield Eyepiece
WHN10X FN 22
Practical Field of
View (mm)
4.4
2.2
1.1
0.44
0.22
Depth of Focus
(μm)
98
18
6.1
1.4
0.73
Objectives
LCD Long Working Distance M Plan Semi Apochromat
LCPLFLN-LCD series
These objectives are designed for making observations through LCD panels and other samples that have a glass substrate. The
correction collar provides aberration correction that can be matched to the thickness of the glass.
LCPLFLN20XLCD
LCPLFLN50XLCD
ø20.32
ø20.32
20
27.5
ø25
t=0.7
41.6
43.4
45.238 *
t=0.7
ø25
ø31
ø15.2
W.D.=0.9
ø29.5
ø17.84
(43.638)
25
38.65
40.45
(42.038)
45.238 *
20
4.5
4.8
ø12.77
W.D.=2.5
ø25
t=0.7
24.5
34.75
36.55
(36.738)
20
ø15
W.D.=7.8
45.238 *
4.5
ø20.32
LCPLFLN100XLCD
ø29.5
ø29.5
ø31
ø31
*Typical value when cover glass thickness is 0.7 mm.
Unit: mm
Objective
Corresponding Glass Thickness (mm)
Correction Collar Indication
W.D. (mm)
Correction System
0
8.3
LCPLFLN20XLCD
0–1.2
0.7
7.8
Correction Collar
1.2
7.4
0
3
UIS2 Objectives
Objective
(magnification)
LCPLFLN20XLCD
LCPLFLN50XLCD
LCPLFLN100XLCD
NA
0.45
0.7
0.85
W.D.**
(mm)
7.8
2.5
0.9
Focal Distance
f (mm)
9
3.6
1.8
Weight
(g)
146
170
185
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
**Value when the correction collar indication is 0.7.
3-6
LCPLFLN50XLCD
0–1.2
0.7
2.5
Correction Collar
1.2
2.2
0
1.2
LCPLFLN100XLCD
0–0.7
0.5
0.98
Correction Collar
0.7
0.9
Widefield Eyepiece
Super Widefield Eyepiece
WHN10X FN 22
SWH10X FN 26.5
Total
Practical Field Depth of
Total
Practical Field Depth of
Magnification of View (mm) Focus (μm) Magnification of View (mm) Focus (μm)
200
1.1
5.2
200
1.3
5.2
500
0.44
1.6
500
0.53
1.6
1000
0.22
0.79
1000
0.27
0.79
Objectives
Super Long Working Distance M Plan Achromat
SLMPLN series
These are high-magnification Plan Achromat objectives with a super long working distance. Three magnifications, 20X, 50X, and 100X,
are available. For 5X or 10X objectives, select from the LMPLFLN series.
SLMPLN50X
ø20.32
ø20.32
ø21.4
36.2
37.2
(37.4)
45
ø17.3
W.D.=7.6
45
W.D.=18
ø16.9
ø26
ø20.32
24.1
26.3
4.9
(27)
19.3
19.8
4.9
(20)
45
W.D.=25
ø16.1
ø25
SLMPLN100X
4.9
SLMPLN20X
ø14.7
ø23.8
ø26
ø26
Unit: mm
UIS2 Objectives
Objective
(magnification)
SLMPLN20X
SLMPLN50X
SLMPLN100X
NA
0.25
0.35
0.6
W.D.
(mm)
25
18
7.6
Focal Distance
f (mm)
9
3.6
1.8
Weight
(g)
56
74
100
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
3-7
Widefield Eyepiece
Super Widefield Eyepiece
WHN10X FN 22
SWH10X FN 26.5
Total
Practical Field Depth of
Total
Practical Field Depth of
Magnification of View (mm) Focus (μm) Magnification of View (mm) Focus (μm)
200
1.1
11.4
200
1.3
11.4
500
0.44
4.2
500
0.53
4.2
1000
0.22
1.3
1000
0.27
1.3
Objectives
IR Long Working Distance M Plan Achromat
LMPLN-IR series
This series is designed for near-infrared microscopy, which is typically used to view the internal structure of silicon wafers.
LMPLN5XIR
LMPLN10XIR
ø20.32
20
W.D.=18
W.D.=23
45
45
(27.05)
18
(21.5)
4.5
4.5
ø20.32
ø20.6
ø14.4
ø24
ø22
ø26
Unit: mm
UIS2 Objectives
Objective
(magnification)
LMPLN5XIR
LMPLN10XIR
NA
0.1
0.3
W.D.
(mm)
23
18
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
3-8
Focal Distance
f (mm)
36
18
Weight
(g)
55
78
Objectives
IR M Plan Achromat
LCPLN-IR series
This series is designed for near-infrared microscopy, which is typically used to view the internal structure of silicon wafers. These
objectives have a correction collar to correct for aberrations based on the thickness of the silicon or glass substrate.
LCPLN20XIR
LCPLN50XIR
19.8
43.4
45
43.8
27.5
19.8
25
31.2
29.5
4.5
(40.5)
45
19.88
24.38
34.4
(36.62)
45
ø20.32
4.5
ø20.32
4.5
ø20.32
LCPLN100XIR
ø25
W.D.=1.2
ø15.2
W.D.=4.5
W.D.=8.3
ø14.64
ø14.94
ø17.7
ø25
ø17.82
ø25
ø29.5
ø29.5
ø29.5
ø31
ø31
ø31
Unit: mm
Silicon thickness correction
Objective
Corresponding Silicon Thickness (mm)
Correction Collar Indication
W.D.* (mm)
Correction System
0
8.3
LCPLN20XIR
0–1.2
0.7
8.2
Correction Collar
1.2
8
LCPLN50XIR
0–1.2
0.6
4.3
Correction Collar
0
4.5
1.2
4.1
LCPLN100XIR
0–1.0
0.5
1.1
Correction Collar
0
1.2
1
1
*Using a 1100 nm laser.
Silicon thickness correction
Objective
Corresponding Glass Thickness (mm)
Correction Collar Indication
W.D.* (mm)
Correction System
0
8.3
LCPLN20XLCD
0–1.2
0.7
7.9
Correction Collar
LCPLN50XLCD
0–1.2
1.2
7.6
0
4.5
LCPLN100XLCD
0–0.7
1.2
3.7
Correction Collar
0
1.2
0.7
0.9
Correction Collar
*Using a 1064 nm laser.
UIS2 Objectives
Objective
(magnification)
LCPLN20XIR
LCPLN50XIR
LCPLN100XIR
NA*
0.45
0.65
0.85
W.D.*
(mm)
8.3
4.5
1.2
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Value when the correction collar indication is 0.
3-9
Focal Distance
f (mm)
9
3.6
1.8
Weight
(g)
149
169
184
Objectives
M Plan Semi Apochromat BD
(BD: Brightfield/Darkfield)
MPLFLN-BD series
This series of Plan Semi Apochromat objectives provides high-level correction for chromatic aberration with a minimum working distance
of 1 mm. Since the exit pupil position of the 5X–150X objectives is standardized, the position of the DIC prism does not have to be
switched when changing the magnification.
MPLFLN5XBD
MPLFLN10XBD
ø26
ø29.5
ø22
ø26.2
ø32
MPLFLN50XBD
35.97
39
39.5
ø28.5
ø32
ø32
MPLFLN150XBD
ø32
ø32
41
(44)
45
ø27.2
W.D.=1
45
41
ø20
W.D.=1
(44)
41
ø20
ø27.2
ø26
4.5
4.5
ø26
4.5
45
(44)
ø22
ø27.5
ø27
MPLFLN100XBD
ø26
ø17
W.D.=3
W.D.=6.5
ø16.8
ø22.4
W.D.=1
(42)
45
34.1
37
36.5
4.5
(38.5)
45
31
30.25
31.5
4.5
(33)
45
ø16
W.D.=12
ø26
4.5
ø26
MPLFLN20XBD
ø20
ø27.2
ø32
Unit: mm
UIS2 Objectives
Objective
(magnification)
MPLFLN5XBD
MPLFLN10XBD
MPLFLN20XBD
MPLFLN50XBD
MPLFLN100XBD
MPLFLN150XBD
NA
0.15
0.3
0.45
0.8
0.9
0.9
W.D.
(mm)
12
6.5
3
1
1
1
Focal Distance
f (mm)
36
18
9
3.6
1.8
1.2
Weight
(g)
95.5
82.8
87.7
99.8
98.9
104.8
Screw: W 26 mm × 0.706 mm (1 in. × 0.03 in.)
3-10
Widefield Eyepiece
Super Widefield Eyepiece
WHN10X FN 22
SWH10X FN 26.5
Total
Practical Field Depth of
Total
Practical Field Depth of
Magnification of View (mm) Focus (μm) Magnification of View (mm) Focus (μm)
50
4.4
59
50
5.3
59
100
2.2
15
100
2.7
15
200
1.1
5.2
200
1.3
5.2
500
0.44
1.3
500
0.53
1.3
1000
0.22
0.73
1000
0.27
0.73
1500
0.15
0.6
1500
0.18
0.6
Objectives
M Plan Semi Apochromat BDP
BDP: Brightfield/Darkfield/Polarized
MPLFLN-BDP series
This series of Plan Semi Apochromat objectives provides high-level correction for chromatic aberrations with a minimum working
distance of 1 mm. Since the exit pupil position of the 5X–100X objectives is standardized, the position of the DIC prism does not have
to be switched when changing the magnification. This series is optimized for brightfield, darkfield, and polarized light observations and
can be used with differential interference contrast.
MPLFLN5XBDP
MPLFLN10XBDP
ø26
ø29.3
ø26
ø32
MPLFLN50XBDP
35.97
39
39.5
4.5
ø22
ø17
W.D.=3
W.D.=6.5
ø22.4
ø22
ø27.5
ø27
ø28.5
ø32
ø32
MPLFLN100XBDP
ø26
41
45
(44)
41
(44)
4.5
4.5
ø26
45
(42)
34.1
37
36.5
ø16.8
ø16
W.D.=12
ø26
45
45
(38.5)
31
30.25
31.5
45
(33)
4.5
4.5
ø26
MPLFLN20XBDP
ø20
W.D.=1
W.D.=1
ø20
ø27.5
ø32
ø27.5
ø32
Unit: mm
UIS2 Objectives
Objective
(magnification)
MPLFLN5XBDP
MPLFLN10XBDP
MPLFLN20XBDP
MPLFLN50XBDP
MPLFLN100XBDP
NA
0.15
0.25
0.4
0.75
0.9
W.D.
(mm)
12
6.5
3
1
1
Focal Distance
f (mm)
36
18
9
3.6
1.8
Weight
(g)
95.5
83.3
88.5
100.5
101.5
Screw: W 26 mm × 0.706 mm (1 in. × 0.03 in.)
3-11
Widefield Eyepiece
Super Widefield Eyepiece
WHN10X FN 22
SWH10X FN 26.5
Total
Practical Field Depth of
Total
Practical Field Depth of
Magnification of View (mm) Focus (μm) Magnification of View (mm) Focus (μm)
50
4.4
59
50
5.3
59
100
2.2
18
100
2.7
18
200
1.1
6.1
200
1.3
6.1
500
0.44
1.4
500
0.53
1.4
1000
0.22
0.73
1000
0.27
0.73
Objectives
Long Working Distance M Plan Semi Apochromat BD
(BD: Brightfield/Darkfield)
LMPLFLN-BD series
This series of long working distance Plan Semi Apochromat objectives provides high-level correction for chromatic aberration and are
suitable for observing larger samples. Since the exit pupil position of the 5X–100X objectives is standardized, the position of the DIC
prism does not have to be switched when changing the magnification.
LMPLFLN5XBD
LMPLFLN10XBD
ø26
ø28
ø21.9
ø28.2
W.D.=12
W.D.=10
ø22.4
31.3
32.2
32.5
4.5
(33)
45
45
ø22
ø15.5
ø16.2
W.D.=15
ø26
30.2
32
(35)
32.3
24
26.2
26.6
45
(30)
5
4.5
ø26
LMPLFLN20XBD
ø23
ø28
ø29.5
ø30
ø30
ø32
ø32
ø32
LMPLFLN50XBD
LMPLFLN100XBD
ø26
36.5
37.3
37.7
(41.7)
45
45
31.8
33
32.7
(34.4)
4.8
4.7
ø26
ø21
W.D.=3.3
W.D.=10.6
ø20.7
ø23
ø28
ø29
ø30.3
ø32
ø32
Unit: mm
UIS2 Objectives
Objective
(magnification)
LMPLFLN5XBD
LMPLFLN10XBD
LMPLFLN20XBD
LMPLFLN50XBD
LMPLFLN100XBD
NA
0.13
0.25
0.4
0.5
0.8
W.D.
(mm)
15
10
12
10.6
3.3
Focal Distance
f (mm)
36
18
9
3.6
1.8
Weight
(g)
81
84
86
85
102
Screw: W 26 mm × 0.706 mm (1 in. × 0.03 in.)
3-12
Widefield Eyepiece
Super Widefield Eyepiece
WHN10X FN 22
SWH10X FN 26.5
Total
Practical Field Depth of
Total
Practical Field Depth of
Magnification of View (mm) Focus (μm) Magnification of View (mm) Focus (μm)
50
4.4
70
50
5.3
70
100
2.2
18
100
2.7
18
200
1.1
6.1
200
1.3
6.1
500
0.44
2.5
500
0.53
2.5
1000
0.22
0.87
1000
0.27
0.87
Objectives
M Plan Achromat BD
(BD: Brightfield/Darkfield)
MPLN-BD series
This series of Plan Achromat objectives provides excellent image flatness up to FN 22.
MPLN5XBD
MPLN10XBD
ø26
41
42.5
45
(43.7)
34.01
37
36.5
4.5
45
(38.5)
W.D.=6.5
ø16.8
ø30.5
ø17
W.D.=1.3
31
30.25
31.5
4.5
(33)
45
ø16
ø29.3
W.D.=12
ø26
(4.5)
ø26
MPLN20XBD
ø22
ø26
ø32
ø23.6
ø27
ø29
ø32
ø32
MPLN100XBD
ø26
ø26
41
42.5
43.33
43.71
45
(44.79)
41
42.5
43.68
45
(44.62)
4.5
4.5
MPLN50XBD
ø10
W.D.=0.21
W.D.=0.38
ø10
ø20.7
ø23.1
ø23
ø27
ø29
ø29
ø32
ø32
Unit: mm
UIS2 Objectives
Objective
(magnification)
MPLN5XBD
MPLN10XBD
MPLN20XBD
MPLN50XBD
MPLN100XBD
NA
0.1
0.25
0.4
0.75
0.9
W.D.
(mm)
12
6.5
1.3
0.38
0.21
Focal Distance
f (mm)
36
18
9
3.6
1.8
Screw: W 26 mm × 0.706 mm (1 in. × 0.03 in.)
3-13
Weight
(g)
137
155
162
157
160
Total
Magnifications
50
100
200
500
1000
Widefield Eyepiece
WHN10X FN 22
Practical Field of
View (mm)
4.4
2.2
1.1
0.44
0.22
Depth of Focus
(μm)
98
18
6.1
1.4
0.73
Microscope Objectives
White Light Interferometry Objective
WLI100XMRTC
This objective is designed to be used with Mirau-style white light interferometers and tolerates high temperatures. The objective has a
working distance of 0.7 mm and an optimized NA of 0.8 that provides improved light gathering.
WLI100XMRTC
ø29
26.3
32.6
39.7
44.98
W.D.=0.7
( 44.28 )
4.9
ø20.32
ø8.9
ø25.5
ø29
unit: mm
Objective
(magnification)
WLI100XMRTC
NA
0.8
W.D.
(mm) *
0.7
Cover Glass
Thickness (mm)
0
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
3-14
Immersion
Spring
Fluorescence **
FN
—
—
—
22.0
Objectives (for Life Science Applications)
■ Definition for Objective Abbreviations
U
U: Universal objectives for
fluorescence (U/BG) and DIC
L: Long working distance
C: Tissue culture observations
through bottles and dishes
M: For no cover observation
PL (Plan)
PL (or PLN):
Plan: Corrects
field curvature of
the periphery on
the image plane
None: Non-Plan
*XL: Extra large; parfocal length
exceeds 45 mm
(e.g. XLUMPLFL20XW 75 mm)
FL
N
ACHN: Achromat: Corrects aberration at 2
wavelengths of blue and red
FLN: Semi Apochromat: Corrects
chromatic aberration in the visible
range (violet to red)
APON: Apochromat: Optimally corrects
chromatic aberration in the entire
visible band (violet to red)
SAPO: Super apochromat: Optimally
corrects chromatic aberration from
violet to NIR
60X
OI
Number:
Objective
magnification
PH
None: Dry
O:
Oil immersion
W:
Water immersion
S:
Silicone immersion
OI:
Oil immersion with iris
None: Brightfield
P:
Polarizing
PH: Phase contrast
PHP: Pre-centered phase contrast
RC: Relief contrast
CY: Equiped with ND filter
TIRF: High-contrast TIRF imaging
340 : High-transmission at 340
nm wave length
■ Objective Notation
Magnification
Infinity-corrected
optical system
Cover glass thickness
(no cover)
Objective series abbeviation (PL: Plan)
Magnification
Objective series abbeviation (PL: Plan)
NA (numerical aperture)
NA (numerical aperture)
Infinity-corrected
optical system
For immersion
(dry if blank)
For observation method
0
FN (Field Number)
0.17
1
FN (Field Number)
2
Cover glass thickness
(no cover)
■ Objective series List
Objective series for standard biological samples
Series
Magnification
UPLSAPO
4X/10X/20X/20XO/40X/60XO/60XW/100XO
PLAPON
1.25X/2X/60XO
4X/10X/20X/40X/40XO/60X/60XOI/100XO/100XOI
UPLFLN
PLFLN
100X
PLN
2X/4X/10X/20X/40X/50XOI/100XO
4XPH/10XPH/20XPH/40XPH/60XOIPH/100XOPH
UPLFLN-PH
PLN-PH
10XPH/20XPH/40XPH/100XOPH
UPLFLN-P
4XP/10XP/20XP/40XP/100XOP
PLN-P/ACHN-P 4XP/10XP/20XP/40XP/100XOP
PLN-CY
2XCY/4XCY/10XCY/20XCY/(FLN) 10XCY
BF
DF
10X/20X/20XO
DIC*
(except 4X)
POL
FL
60XO
(except 4X)
60XO 60XO
10X/20X/40X/60XOI/100XOI
10X/20X/40X/50XOI
10X/20X/40X/50XOI
10XPH/20XPH/40XPH/60XOIPH
(excpet 100XOPH)
(except 4XP)
10XP/20XP/40XP
10XP/20XP/40XP
FN
26.5
26.5
26.5
26.5
22
26.5
22
26.5
22
22
Remarks
PLFLN10XCY FN 26.5
*These objectives are suitable for standard biological samples embeded on a glass slide with a 0.17 mm cover slip and are mainly used with upright microscopes.
Objective series for cultured samples
Series
Magnification
BF
DF
DIC
POL
FL
LUCPLFLN
20X/40X/60X
LUCPLFLN-RC/
10XRC/20XRC/40XRC
UCPLFLN-RC
LUCPLFLN-PH/
10XPH/20XPH/40XPH/60XPH
UCPLFLN-PH
CPLN-PH/
10XPH/20XPH/40XPH
LCACHN-PH
CPLN-RC/
10XRC/20XRC/40XRC
LCACHN-RC
These objectives are suitable for cultured tissue/cell observation in a dish, bottle, or micro plate and are mainly used with inverted microscopes.
Objective series for special applications
Series
Magnification
LUMPLFLN/
10XW/20XW/40XW/60XW
UMPLFLN
XLUMPLFLN
20XW
APON 340
20XW/40XO/40XW
TIRF Objectives 60XO/100XHO/100XO/150XO
BF
DF
DIC
POL
FL
FN
22
Remarks
22
22
22
22
FN
10XW/20XW
26.5
20XW/40XW
22
22
22
Remarks
Perocal length 75 mm
■ Features of Each Objective Series (please refer to the following pages for details on each objective.)
UPLSAPO: Universal Plan Super Apochromat — p. 3-16
PLAPON: Plan Apochromat — p. 3-18
UPLFLN: Universal Plan Semi Apochromat/Plan Semi Apochromat — p. 3-19
PLN: Plan Achromat — p. 3-21
UPLFLN-PH UPlanFl-P Universal Plan Semi Apochromat for Phase Contrast — p. 3-23
PLN-PH: Plan Achromat for Phase Contrast — p. 3-24
UPLFLN-P: UPlanFl-P Universal Plan Semi Apochromat for Polarizing. — p. 3-25
PLN/ACHN-P: Achromat for Polarizing. — p. 3-26
PLN-CY: Plan Achromat (ND) — p. 3-27
LUCPLFLN, UCPLFLN: Long Working Distance Universal Plan Semi Apochromat — p. 3-28
LUCPLFLN/UCPLFLN-RC: Long Working Distance Universal Plan Semi Apochromat for Relief Contrast — p. 3-29
LUCPLFLN/UCPLFLN-PH: Long Working Distance Universal Plan Semi Apochromat for Phase Contrast — p. 3-30
CPLN/LCACHN-PH: Culture Specimen Objectives for Phase Contrast — p. 3-31
CPLN/LCACHN-RC: Culture Specimen Objectives for Relief Contrast — p. 3-32
LUMPLFLN/UMPLFLN: No Cover Water Immersion for Fixed Stage Upright Microscope — p. 3-33
XLUMPLFLN: No Cover Water Immersion for Fixed Stage Upright Microscope — p. 3-34
APON 340: Universal Apochromat — p. 3-35
TIRF Objectives — p. 3-36
3-15
Microscope Objectives
Universal Plan Super Apochromat
UPLSAPO series
The UPLSAPO Super Apochromat objectives fully compensate for both spherical and chromatic aberrations from the UV to the nearinfrared region. This enables the acquisition of sharp, clear images without color shift in brightfield, Nomarski DIC, and fluorescence
observations. For quality and performance, this objective series is a splendid solution for numerous digital imaging applications.
UPLSAPO4X
UPLSAPO10X2
ø20.32
34.8
39.6
( 41.78 )
45.06
CG= 0.17
W.D.=3.1
25.3
( 31.89 )
CG= 0.17
W.D.=13
45.06
4.7
4.5
ø20.32
ø15
ø28
ø11.5
ø16.4
ø28
UPLSAPO20X
UPLSAPO20XO
ø20.32
36.9
42.7
( 44.69 )
4.5
45.06
W.D.=0.2
CG= 0.17
41
37.4
( 44.29 )
W.D.=0.6
CG= 0.17
45.06
4.5
ø20.32
ø9
ø18.3
ø7.5
ø15.8
ø28
ø24.5
unit: mm
Objectives
(magnification)
NA
W.D.
(mm) *
Cover Glass
Thickness (mm)
Immersion
Spring
Fluorescence **
FN
UPLSAPO4X
0.16
UPLSAPO10X2
0.40
13.0
—
—
—
U/BG/IR
26.5
3.1
0.17
—
—
U/BG/IR
UPLSAPO20X
26.5
0.75
0.6
0.17
—
Yes
U/BG/IR
UPLSAPO20XO
26.5
0.85
0.2
—
Oil
Yes
U/BG/IR
26.5
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
3-16
Microscope Objectives
Universal Plan Super Apochromat
UPLSAPO series
The UPLSAPO Super Apochromat objectives fully compensate for both spherical and chromatic aberrations from the UV to the nearinfrared region. This enables the acquisition of sharp, clear images without color shift in brightfield, Nomarski DIC, and fluorescence
observations. For quality and performance, this objective series is a splendid solution for numerous digital imaging applications.
UPLSAPO40X2
UPLSAPO60XO
ø28
ø20.32
19.4
ø8.3
CG= 0.17
36.9
41.7
W.D.=0.15
45.06
( 44.74 )
36
41.7
W.D.=0.18
45.06
( 44.71 )
26.1
4.7
4.7
ø28
ø20.32
ø22
ø30
UPLSAPO60XW
ø22
ø30
UPLSAPO100XO
ø28
ø20.32
36.9
41.7
CG= 0.17
W.D.=0.13
45.06
( 44.76 )
19.4
36.3
39.9
42.5
W.D.=0.28
( 44.61 )
24.5
4.7
4.5
ø28
ø20.32
ø7.5
CG= 0.17
45.06
ø8.5
CG= 0.17
ø31
ø15.8
ø6.6
ø22
ø30
ø24.5
ø31.5
unit: mm
Objectives
(magnification)
NA
W.D.
(mm) *
Cover Glass
Thickness (mm)
UPLSAPO40X2
0.95
0.18
0.11–0.23
—
Yes
U/BG/IR
26.5
UPLSAPO60XO
1.35
0.15
0.17
Oil
Yes
U/BG/IR
26.5
UPLSAPO60XW
1.20
0.28
0.13–0.21
Water
Yes
U/BG/IR
26.5
UPLSAPO100XO
1.40
0.13
0.17
Oil
Yes
U/BG/IR
26.5
Immersion
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
3-17
Spring
Fluorescence **
FN
Microscope Objectives
Plan Apochromat
PLAPON series
The PLAPON Apochromat objectives display flat images from violet to the near-infrared region of the spectrum. For quality and
performance, this objective series is a splendid solution for numerous digital imaging applications.
PLAPON1.25X
PLAPON2X
PLAPON60XO
ø20.32
ø20.32
ø28
36.9
41.7
(44.74)
45.06
CG=0.17
ø28
W.D.=0.15
33.8
45.06
(38.69)
4.5
ø18.3
CG=0.17
ø27.2
W.D.=6.2
38.2
(39.89)
4.7
W.D.=5
CG=0.17
45.06
4.7
ø20.32
ø18.5
ø8
ø22
ø30
ø28
unit: mm
Objectives
(magnification)
NA
W.D.
(mm) *
Cover Glass
Thickness (mm)
Immersion
Spring
Fluorescence **
FN
PLAPON1.25X
0.04
5.0
—
—
—
(BG)(/IR)
26.5
PLAPON2X
0.08
6.2
—
—
—
(BG)(/IR)
26.5
PLAPON60XO
1.42
0.15
0.17
Oil
Yes
(U)/BG/IR ***
26.5
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
***IR excitation might cause the objective to go slightly out of focus.
3-18
Microscope Objectives
Universal Plan Semi Apochromat/Plan Semi Apochromat
UPLFLN, PLFLN series
The UPLFLN and PLFLN objectives display flat images from violet up to the near-infrared region of the spectrum. With their high signalto-noise (S/N) ratio, high resolution, and splendid contrast, they are especially effective in brightfield and Nomarski DIC observations.
For quality and performance, this objective series is a splendid solution for numerous digital imaging applications.
UPLFLN4X
UPLFLN10X2
35.2
40.5
45.06
W.D.=2.1
W.D.=10
ø13.6
ø24
UPLFLN40X
ø9.4
CG=0.17
CG=0.17
ø18
CG=0.17
(42.79)
24.8
(34.89)
45.06
24.8
(27.89)
45.06
W.D.=17
4.5
ø20.32
4.5
ø20.32
4.5
ø20.32
UPLFLN20X
ø24
ø15.8
ø26
UPLFLN40XO
ø28
ø20.32
29
39
W.D.=0.2
43
(44.69)
45.06
35.3
40.5
W.D.=0.51
45.06
(44.38)
4.7
4.5
ø20.32
ø7.8
CG=0.17
CG=0.17
ø8.5
ø16
ø26
ø19.3
ø30
unit: mm
Objectives
(magnification)
UPLFLN4X
UPLFLN10X2
UPLFLN20X
UPLFLN40X
UPLFLN40XO
0.13
W.D.
(mm) *
17
Cover Glass
Thickness (mm)
—
0.30
10
—
0.50
2.1
0.17
0.75
0.51
0.17
1.30
0.2
0.17
NA
Immersion
Spring
Fluorescence **
—
—
(U)(/BG)(/IR)
26.5
—
—
U/BG/IR ***
26.5
—
Yes
U/BG/IR ***
26.5
—
Yes
U/BG/IR ***
26.5
Oil
Yes
U/BG/IR ***
26.5
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
***IR excitation might cause the objective to go slightly out of focus.
3-19
FN
Microscope Objectives
Universal Plan Semi Apochromat/Plan Semi Apochromat
UPLFLN, PLFLN series
The UPLFLN and PLFLN objectives display flat images from violet up to the near-infrared region of the spectrum. With their high S/N
ratio, high resolution, and splendid contrast, they are especially effective in brightfield and Nomarski DIC observations. For quality and
performance, this objective series is a splendid solution for numerous digital imaging applications.
UPLFLN60X
UPLFLN60XOI
ø28
ø20.32
20
38.7
W.D.=0.12
41.8
45.06
(44.77)
26.5
19.4
37
42.2
W.D.=0.2
45.06
(44.69)
24.4
4.7
4.7
ø28
ø20.32
CG=0.17
ø17
ø29
ø3.7
ø20.1
ø30.5
ø31
ø31
UPLFLN100XO2
UPLFLN100XOI2
PLFLN100X
ø28
ø20.32
19.9
35.5
45.06
W.D.=0.23
W.D.=0.2
ø7
ø6
ø17.2
ø28
CG=0.17
ø7
CG=0.17
CG=0.17
40.3
24.3
20
37.1
41
(44.69)
45.06
36.2
41.7
W.D.=0.2
45.06
(44.69)
26.5
4.5
4.8
ø26
ø20.32
4.5
ø26
ø20.32
(44.66)
CG=0.17
ø8.5
ø18.2
ø29
ø19.5
ø29
ø31
unit: mm
Objectives
(magnification)
UPLFLN60X
UPLFLN60XOI
0.90
W.D.
(mm) *
0.2
Cover Glass
Thickness (mm)
0.11–0.23
—
Yes
U/BG/IR ***
26.5
1.25–0.65
0.12
0.17
Oil
Yes
U/BG/IR ***
26.5
NA
Immersion
Spring
Fluorescence **
FN
UPLFLN100XO2
1.30
0.2
0.17
Oil
Yes
U/BG/IR ***
26.5
UPLFLN100XOI2
1.3–0.6
0.2
0.17
Oil
Yes
U/BG/IR ***
26.5
0.95
0.2
0.14–0.2
—
Yes
BG
26.5
PLFLN100X
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
***IR excitation might cause the objective to go slightly out of focus.
3-20
Microscope Objectives
Plan Achromat
PLN series
The PLN standard objectives provide excellent field flatness during fluorescence, darkfield, and brightfield observation in transmitted
light. These objectives are well-suited to clinical laboratory and examination work.
PLN2X
PLN4X
ø20.32
22
( 26.39 )
W.D.=18.5
45.06
35.4
ø15.8
CG= 0.17
45.06
( 39.09 )
ø16.8
CG= 0.17
W.D.=5.8
4.5
4.5
ø20.32
ø24
PLN10X
ø24
PLN20X
ø20.32
36.9
41.3
( 43.69 )
45.06
W.D.=1.2
32.7
28.6
ø12
ø6
CG= 0.17
CG= 0.17
W.D.=10.6
45.06
( 34.29 )
4.8
4.5
ø20.32
ø16
ø24
ø15.6
ø24
unit: mm
Objectives
(magnification)
PLN2X
PLN4X
0.06
W.D.
(mm) *
5.8
Cover Glass
Thickness (mm)
—
0.10
18.5
—
PLN10X
0.25
10.6
PLN20X
0.40
1.2
NA
Immersion
Spring
Fluorescence **
FN
—
—
(BG)
22.0
—
—
(BG)
22.0
—
—
—
(BG)
22.0
0.17
—
Yes
(BG)
22.0
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
3-21
Microscope Objectives
Plan Achromat
PLN series
The PLN standard objectives provide excellent field flatness during fluorescence, darkfield, and brightfield observation in transmitted
light. These objectives are well-suited to clinical laboratory and examination work.
PLN40X
PLN50XOI
ø26
ø20.32
20
26.5
37.2
W.D.=0.2
41
( 44.69 )
45.06
36.9
41.3
W.D.=0.6
45.06
( 44.29 )
4.8
4.5
ø20.32
ø6
CG= 0.17
CG= 0.17
ø7.5
ø15.6
ø24
ø18.2
ø29
PLN100XO
CG= 0.17
38.4
42.8
W.D.=0.15
45.06
( 44.74 )
4.5
ø20.32
ø6.6
ø15.6
ø24
unit: mm
Objectives
(magnification)
PLN40X
0.65
W.D.
(mm) *
0.6
—
Yes
(BG)
22.0
PLN50XOI
0.9–0.5
0.2
—
Oil
Yes
(BG)
22.0
PLN100XO
1.25
0.15
—
Oil
Yes
(BG)
22.0
NA
Cover Glass
Thickness (mm)
0.17
Immersion
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
3-22
Spring
Fluorescence **
FN
Microscope Objectives
Universal Plan Semi Apochromat for Phase Contrast
UPLFLN-PH series
The UPLFLN-PH objectives are especially effective in phase contrast observations with their high S/N ratio, high resolution, and splendid
contrast. These objectives display flat images from violet up to the near-infrared region of the spectrum.
UPLFLN4XPH
UPLFLN10X2PH
ø20.32
ø24
35.2
40.5
45.06
W.D.=2.1
ø13.6
ø9.4
CG= 0.17
CG= 0.17
ø18
( 42.79 )
24.8
( 34.89 )
CG= 0.17
W.D.=10
W.D.=17
45.06
45.06
24.8
( 27.89 )
4.5
4.5
ø20.32
4.5
ø20.32
UPLFLN20XPH
ø24
ø15.8
ø26
UPLFLN40XPH
UPLFLN60XOIPH
ø26
ø20.32
4.5
ø28
ø20.32
ø16
ø26
36.2
41.7
45.06
W.D.=0.2
CG= 0.17
CG= 0.17
ø8.5
( 44.69 )
20
26.5
38.7
W.D.=0.12
41.8
( 44.77 )
45.06
35.3
40.5
CG= 0.17
W.D.=0.51
45.06
( 44.38 )
4.7
4.5
ø20.32
UPLFLN100XO2PH
ø3.7
ø20.1
ø7
ø17.2
ø30.5
ø28
ø31
unit: mm
Objectives
(magnification)
UPLFLN4XPH
UPLFLN10X2PH
0.13
W.D.
(mm) *
17
Cover Glass
Thickness (mm)
—
0.30
10
—
UPLFLN20XPH
0.50
2.1
UPLFLN40XPH
0.75
UPLFLN60XOIPH
UPLFLN100XO2PH
NA
Immersion
Spring
Fluorescence **
FN
—
—
U/BG/IR ***
26.5
—
—
U/BG/IR ***
26.5
0.17
—
Yes
U/BG/IR ***
26.5
0.51
0.17
—
Yes
U/BG/IR ***
26.5
1.25–0.65
0.12
0.17
Oil
Yes
U/BG/IR ***
26.5
1.30
0.2
0.17
Oil
Yes
U/BG/IR ***
26.5
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
***IR excitation might cause the objective to go slightly out of focus.
3-23
Microscope Objectives
Plan Achromat for Phase Contrast
PLN-PH series
The PLN-PH standard objectives provide excellent field flatness during phase contrast observation in transmitted light.
PLN10XPH
PLN20XPH
ø20.32
36.9
41.3
W.D.=1.2
45.06
( 43.69 )
28.6
32.7
CG= 0.17
CG= 0.17
W.D.=10.6
45.06
( 34.29 )
4.8
4.5
ø20.32
ø10.5
ø16
ø6
ø15.6
ø24
ø24
PLN40XPH
PLN100XOPH
38.4
42.8
( 44.74 )
45.06
W.D.=0.15
41.3
36.9
CG= 0.17
CG=0.17
W.D.=0.6
45.06
( 44.29 )
4.5
ø20.32
4.5
ø20.32
ø7.5
ø15.6
ø7
ø15.6
ø24
ø24
unit: mm
Objectives
(magnification)
PLN10XPH
PLN20XPH
0.25
W.D.
(mm) *
10.6
Cover Glass
Thickness (mm)
—
0.40
1.2
0.17
PLN40XPH
0.65
0.6
PLN100XOPH
1.25
0.15
NA
Immersion
Spring
Fluorescence **
FN
—
—
(BG)
22.0
—
Yes
(BG)
22.0
0.17
—
Yes
(BG)
22.0
—
Oil
Yes
(BG)
22.0
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
3-24
Microscope Objectives
Universal Plan Semi Apochromat for Polarizing
UPLFLN-P series
The UPLFLN-P universal objectives display flat images from violet up to the near-infrared region of the spectrum. These objectives
reduce internal strain to a minimum and are designed for polarizing, Nomarski DIC, brightfield, and fluorescence microscopy.
UPLFLN4XP
UPLFLN10XP
UPLFLN20XP
ø20.32
ø20.32
4.5
ø24
35.2
40.5
45.06
W.D.=2.1
ø13.6
ø9.4
CG= 0.17
ø18
( 42.79 )
24.8
CG= 0.17
CG= 0.17
W.D.=10
( 34.89 )
45.06
24.8
W.D.=17
45.06
( 27.89 )
4.5
4.5
ø20.32
ø24
ø15.8
ø26
UPLFLN40XP
UPLFLN100XOP
ø26
ø20.32
36.2
W.D.=0.2
41.7
( 44.69 )
45.06
40.5
35.3
ø8.5
CG= 0.17
CG= 0.17
W.D.=0.51
45.06
( 44.38 )
4.5
4.5
ø20.32
ø16
ø26
ø7
ø17.2
ø28
unit: mm
Objectives
(magnification)
UPLFLN4XP
UPLFLN10XP
0.13
W.D.
(mm) *
17
Cover Glass
Thickness (mm)
—
0.30
10
—
UPLFLN20XP
0.50
2.1
UPLFLN40XP
0.75
UPLFLN100XOP
1.30
NA
Immersion
Spring
Fluorescence **
FN
—
—
(U)(/BG)
26.5
—
—
U/BG
26.5
0.17
—
Yes
U/BG
26.5
0.51
0.17
—
Yes
U/BG
26.5
0.2
0.17
Oil
Yes
U/BG
26.5
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. “( )” indicates that fluorescence might be slightly darker.
3-25
Microscope Objectives
Achromat for Polarizing
PLN-P, ACHN-P series
The PLN-P and ACHN-P are cost-efficient objectives designed for transmitted polarized light observations.
PLN4XP
ACHN10XP
ø20.32
ACHN20XP
ø20.32
34.6
45.06
W.D.=3
ø7.1
ø18
ø24
ø9.6
CG= 0.17
ø15.6
( 41.89 )
30.8
34
( 38.89 )
45.06
CG= 0.17
ø7.5
CG=0.17
W.D.=6
36.9
41.3
W.D.=0.6
45.06
( 44.29 )
4.5
4.5
4.5
ø20.32
ø24
ø24
ACHN40XP
ACHN100XOP
ø20.32
38.4
42.8
( 44.76 )
W.D.=0.13
45.06
36.9
ø5.6
ø3.3
CG= 0.17
41.3
( 44.44 )
CG= 0.17
W.D.=0.45
45.06
4.5
4.5
ø20.32
ø15.6
ø15.6
ø24
ø24
unit: mm
Objectives
(magnification)
PLN4XP
ACHN10XP
ACHN20XP
ACHN40XP
ACHN100XOP
0.10
W.D.
(mm) *
18.5
Cover Glass
Thickness (mm)
—
—
—
(BG)
22.0
0.25
6
—
—
—
(BG)
22.0
0.40
3
0.17
—
—
(BG)
22.0
0.65
0.45
0.17
—
Yes
(BG)
22.0
1.25
0.13
—
Oil
Yes
(BG)
22.0
NA
Immersion
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
3-26
Spring
Fluorescence **
FN
Microscope Objectives
Plan Achromat (ND)
PLN-CY, PLFLN-CY series
The PLN-CY and PLFLN-CY standard objectives provide excellent field flatness. These objectives, equipped with neutral density (ND)
filters, provide the same level of brightness, even if the magnification is changed, thereby removing the need for brightness adjustment.
PLN2XCY
PLN4XCY
ø20.32
PLN10XCY
ø20.32
45.06
28.6
32.7
W.D.=10.6
W.D.=18.5
CG= 0.17
ø15.8
ø10.5
CG= 0.17
ø24
( 34.29 )
22
( 26.39 )
45.06
35.4
( 39.09 )
ø16.8
CG= 0.17
W.D.=5.8
45.06
4.5
4.5
4.5
ø20.32
ø24
ø16
ø24
PLN20XCY
PLFLN10XCY
ø20.32
24.8
W.D.=10
( 34.89 )
45.06
41.3
36.9
CG= 0.17
CG= 0.17
W.D.=1.2
45.06
( 43.69 )
4.8
4.5
ø20.32
ø6
ø15.6
ø13.6
ø24
ø24
unit: mm
Objectives
(magnification)
PLN2XCY
PLN4XCY
0.06
W.D.
(mm) *
5.8
Cover Glass
Thickness (mm)
—
0.10
18.5
—
PLN10XCY
0.25
10.6
PLN20XCY
0.40
PLFLN10XCY
0.30
NA
Immersion
Spring
Fluorescence **
FN
—
—
—
22.0
—
—
—
22.0
—
—
—
(BG)
22.0
1.2
0.17
—
—
(BG)
22.0
9.9
—
—
—
BG
26.5
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
3-27
Microscope Objectives
Long Working Distance Universal Plan Semi Apochromat
LUCPLFLN series
The LUCPLFLN long working distance, universal objectives display flat images from violet up to the near-infrared region of the spectrum.
These objectives are dedicated to tissue culture observations through bottles and dishes, offering high contrast and splendid resolution
in brightfield, DIC, and fluorescence observations.
LUCPLFLN20X
LUCPLFLN40X
ø26
ø26
ø20.32
ø20.32
24.3
36
39.9
( 42.64 )
45.34
W.D.=1.7
ø9.5
ø19
ø27
ø9
CG= 1
ø21.8
17.5
4.5
17.5
35.3
39
( 40.94 )
45.34
CG= 1
ø12.1
W.D.=3.4
24.3
17.4
24.4
33.1
35.5
( 36.9 )
W.D.=7.44
4.7
4.5
ø26
ø20.32
CG= 1
45.34
LUCPLFLN60X
ø17.4
ø26
ø26
ø29
ø29
unit: mm
Objectives
(Magnification)
LUCPLFLN20X
LUCPLFLN40X
LUCPLFLN60X
0.45
W.D.
(mm) *
7.8–6.6
Cover Glass
Thickness (mm)
0–2
0.60
4–2.7
0–2
0.70
2.2–1.5
0.1–1.3
NA
Immersion
Spring
Fluorescence **
FN
—
—
U/BG/IR ***
22.0
—
—
U/BG/IR ***
22.0
—
—
U/BG/IR ***
22.0
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV excitation, BG: Visible Excitation, IR : Near IR. "( )" indicates that fluorescence might be slightly darker.
***IR excitation might cause the objective to go slightly out of focus.
3-28
Microscope Objectives
Long Working Distance Universal Plan Semi Apochromat for Relief Contrast
CPLFLN-RC, LUCPLFLN-RC series
The CPLFLN-RC and LUCPLFLN-RC long working distance, universal objectives display flat images from violet up to the near-infrared
region of the spectrum. These objectives are designed for the observation of living cells, including oocytes. Plastic
vessels can be used with these objectives for relief contrast observations.
CPLFLN10XRC
LUCPLFLN20XRC
ø26
ø20.32
ø26
17.5
35.3
39
( 40.71 )
45.37
CG= 1
ø12.1
CG= 1
CG= 1.5
ø13.6
W.D.=3.66
24.3
17.4
24.5
33.2
35.5
( 36.9 )
45.37
W.D.=7.47
29.4
( 35.04 )
4.5
4.5
ø26
ø20.32
W.D.=9
45.54
4.5
ø20.32
LUCPLFLN40XRC
ø21.8
ø9.5
ø19
ø27
ø26
ø29
ø29
unit: mm
Objectives
(magnification)
CPLFLN10XRC
LUCPLFLN20XRC
LUCPLFLN40XRC
0.30
W.D.
(mm) *
9 ***
Cover Glass
Thickness (mm)
—
—
—
BG
22.0
0.45
7.8–6.6
0–2
—
—
U/BG/IR ****
22.0
0.60
4.2–3.0
0–2
—
—
U/BG/IR ****
22.0
NA
Immersion
Spring
Fluorescence **
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
***Defined by 1 mm bottom thickness of a plastic container and 0.5 mm bottom thickness of a glass heat plate (depends on the shape of container).
****IR excitation might cause the objective to go slightly out of focus.
3-29
FN
Microscope Objectives
Long Working Distance Universal Plan Semi Apochromat for Phase Contrast
CPLFLN-PH, LUCPLFLN-PH series
The CPLFLN-PH and LUCPLFLN-PH long working distance, universal objectives display flat images from violet up to the near-infrared
region of the spectrum. These objectives are specifically designed for culture specimens and are optimized to produce splendid phase
contrast images, regardless of the thickness and material of the culture vessel.
CPLFLN10XPH
LUCPLFLN20XPH
ø26
ø20.32
ø12.1
17.5
35.3
39
( 40.71 )
45.37
CG= 1
ø24
ø9.5
CG= 1
CG= 1
ø13.6
W.D.=3.66
24.3
17.4
24.5
33.2
35.5
( 36.9 )
45.37
W.D.=7.47
24.8
( 34.87 )
4.7
4.5
ø26
ø20.32
W.D.=9.5
45.37
4.5
ø20.32
LUCPLFLN40XPH
ø21.8
ø19
ø27
ø26
ø29
ø29
LUCPLFLN60XPH
ø26
17.5
36.1
40.1
( 42.63 )
45.37
CG= 1
W.D.=1.74
24.3
4.5
ø20.32
ø8.5
ø17.4
ø26
ø29
unit: mm
Objectives
(magnification)
CPLFLN10XPH
LUCPLFLN20XPH
0.30
W.D.
(mm) *
9.5 ***
Cover Glass
Thickness (mm)
1
0.45
7.8–6.6
0–2
LUCPLFLN40XPH
0.60
4.2–3.0
LUCPLFLN60XPH
0.70
2.2–1.5
NA
Immersion
Spring
—
—
BG
22.0
—
—
U/BG/IR ****
22.0
0–2
—
—
U/BG/IR ****
22.0
0.1–1.3
—
—
U/BG/IR ****
22.0
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
***Defined by 1 mm bottom thickness of a plastic container.
****IR excitation might cause the objective to go slightly out of focus.
3-30
Fluorescence **
FN
Microscope Objectives
Culture Specimen Objectives for Phase Contrast
CPLN-PH, LCACHN-PH series
The CPLN-PH and LCACHN-PH standard objectives provide excellent field flatness during phase contrast observation in transmitted
light.
CPLN10XPH
LCACHN20XPH
ø8
ø14.2
ø24
34.5
ø6.9
CG= 1
ø16
( 42.17 )
45.37
W.D.=2.2
CG= 1
ø10.5
39.6
4.5
32.9
38
( 41.17 )
W.D.=3.2
45.37
28.5
32.7
( 34.37 )
CG= 1
W.D.=10
45.37
ø20.32
4.5
ø20.32
4.5
ø20.32
LCACHN40XPH
ø14.2
ø24
ø24
unit: mm
Objectives
(magnification)
CPLN10XPH
LCACHN20XPH
LCACHN40XPH
0.25
W.D.
(mm) *
10 ***
Cover Glass
Thickness (mm)
—
—
—
BG
22.0
0.40
3.2 ***
1
—
—
BG
22.0
0.55
2.2 ***
1
—
—
BG
22.0
NA
Immersion
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
***Defined by 1 mm bottom thickness of a plastic container.
3-31
Spring
Fluorescence **
FN
Microscope Objectives
Culture Specimen Objectives for Relief Contrast
CPLN-RC, LCACHN-RC series
The CPLN-RC and LCACHN-RC standard objectives provide excellent field flatness during relief contrast observation in transmitted
light. These objectives are designed for the observation of living cells, including oocytes, in plastic vessels.
LCACHN20XRC
ø24
ø20.32
34.5
45.54
W.D.=1.9
ø6.9
CG= 1.5
ø26
38.8
32.6
( 41.24 )
ø8
CG= 1.5
ø13.6
36.9
4.8
4.8
ø24
ø20.32
W.D.=2.8
45.54
29.4
( 35.04 )
CG= 1.5
W.D.=9
45.54
4.5
ø20.32
LCACHN40XRC
( 42.14 )
CPLN10XRC
ø17
ø17
ø25.6
ø25.6
unit: mm
Objectives
(magnification)
CPLN10XRC
LCACHN20XRC
LCACHN40XRC
0.25
W.D.
(mm) *
9.7 ***
Cover Glass
Thickness (mm)
—
0.40
2.8 ***
1.5
0.55
1.9 ***
1.5
NA
Immersion
Spring
Fluorescence **
FN
—
—
BG
22.0
—
—
BG
22.0
—
—
BG
22.0
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
***Defined by 1 mm bottom thickness of a plastic container and 0.5 mm bottom thickness of a glass heat plate (depends on the shape of container).
3-32
Microscope Objectives
No Cover Water Immersion for a Fixed Stage Upright Microscope
UMPLFLN-W, LUMPLFLN-W series
The UMPLFLN, LUMPLFLN-W, and LUMFLN series of long working distance, water immersion objectives display flat images from high
transmission factors up to the near-infrared region of the spectrum. These objectives achieve splendid DIC and fluorescence from the
visible range to infrared and are ideal for fluorescence imaging of brain tissue, as well as other tissue and specimens.
UMPLFLN10XW
UMPLFLN20XW
ø24
ø24
ø20.32
ø20.32
ø15.7
37.3
4.5
( 41.69 )
45
ø15.7
ø21
ø21
ø7.2
W.D.=3.3
45
( 41.5 )
36.7
ø7
W.D.=3.5
( 41.5 )
36.7
4.5
4.5
ø24
ø20.32
ø7
W.D.=3.5
45
LUMPLFLN40XW
ø15.8
ø21
LUMPLFLN60XW
ø24
37.8
42.7
45
W.D.=2
( 42.95 )
4.5
ø20.32
ø6.6
ø20
ø22
unit: mm
Objectives
(magnification)
UMPLFLN10XW
UMPLFLN20XW
0.30
W.D.
(mm) *
3.50
Cover Glass
Thickness (mm)
0
0.50
3.50
0
LUMPLFLN40XW
0.8
3
LUMPLFLN60XW
1.00
2
NA
Immersion
Spring
Fluorescence **
FN
Water
—
U/BG
26.5
Water
—
U/BG
26.5
0
Water
—
U/BG
26.5
0
Water
—
U/BG
26.5
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
3-33
Microscope Objectives
No Cover Water Immersion for a Fixed Stage Upright Microscope
XLUMPLFLN20XW
The XLUMPLFLN-W high NA, long working distance objectives display flat images from high transmission factors up to the near-infrared
region of the spectrum. These objectives achieve splendid DIC and fluorescence from the visible range to infrared. These objectives
enable the measurement of cell membrane electric potential since the design of the objectives provides easy access to patch clamp
electrodes.
XLUMPLFLN20XW ***
65.6
W.D.=2.02
( 2.03 )
75
( 70.95 )
5.8
ø25
ø10.5
ø23.9
ø30
unit: mm
Objectives
(magnification)
NA
W.D.
(mm) *
Cover Glass
Thickness (mm)
Immersion
Spring
Fluorescence **
FN
XLUMPLFLN20XW ***
1.00
2
0
Water
—
U/BG/IR ****
22.0
Screw: W 25 mm × 0.75 mm (1 in. × 0.03 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
***Special dedicated nosepiece needed (WI-SNPXLU).
****IR excitation might cause the objective to go slightly out of focus.
3-34
Microscope Objectives
Universal Apochromat
UAPON 340 series
The UAPON340 objectives feature high transmission of 340 nm wavelength light, providing maximum performance in fluorescence
microscopes using UV excitation.
UAPON20XW340
UAPON40XO340-2
UAPON40XW340
ø28
ø28.5
ø20.32
ø20.32
ø22
ø7
ø22
ø30
19.7
24.7
29.5
36.5
40.3
ø6
CG= 0.17
ø8.1
( 44.64 )
4.7
CG= 0.17
W.D.=0.1
W.D.=0.25
45.06
36.9
41.7
( 44.79 )
45.06
36.9
41.7
CG= 0.17
W.D.=0.35
45.06
( 44.54 )
4.5
4.5
ø28
ø20.32
ø22.5
ø30
ø30
ø31
unit: mm
Objectives
(magnification)
UAPON20XW340
UAPON40XO340-2
UAPON40XW340
0.70
W.D.
(mm) *
0.35
Cover Glass
Thickness (mm)
0.17
Water
Yes
U/BG
22.0
1.35
0.1
0.17
Oil
Yes
U/BG
22.0
1.15
0.25
0.13-0.25
Water
Yes
U/BG
22.0
NA
Immersion
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
3-35
Spring
Fluorescence **
FN
Microscope Objectives
TIRF Objectives
APON, UAPON series
These objectives feature the highest NA to create an evanescent wave field for high-contrast TIRF images.
APON60XOTIRF
APON100XHOTIRF ***
UAPON100XOTIRF
ø28.5
ø28
ø20.32
ø20.32
26.9
40.7
42.2
43.2
( 44.8 )
45.06
W.D.=0.09
ø7.8
CG= 0.17
ø18.5
22.4
4.7
19.2
40.7
42.2
45.07
W.D.=0.08
43.2
ø7.5
CG= 0.15
CG= 0.17
ø7.8
( 44.84 )
25.2
22.4
40.7
43
41.3
W.D.=0.11
45.06
( 44.78 )
26.9
4.7
4.7
ø28
ø20.32
ø22.6
ø22.6
ø23.7
ø23.7
ø30.5
ø30.5
ø30.5
ø31.5
ø31.5
ø31.5
ø23.7
UAPON150XOTIRF
ø28
22.4
40.6
41.5
42.6
W.D.=0.08
45.06
( 44.81 )
26.9
4.7
ø20.32
CG= 0.17
ø5.6
ø18.1
ø23.3
ø30.5
ø31.5
unit: mm
Objectives
(magnification)
APON60XOTIRF
APON100XHOTIRF ***
1.49
W.D.
(mm) *
0.1
Cover Glass
Thickness (mm)
0.13-0.19
1.70
0.08
0.15
UAPON100XOTIRF
1.49
0.1
0.13-0.19
UAPON150XOTIRF
1.45
0.08
0.13-0.19
NA
Immersion
Spring
Fluorescence **
FN
Oil
—
(U)/BG
22.0
Special Oil
—
BG
22.0
Oil
—
U/BG
22.0
Oil
—
U/BG
22.0
Screw: W 20.32 mm × 0.706 mm (0.8 in. × 1/36 in.)
*Defined with cover glass thickness shown as “CG” in the above drawings.
**U: UV Excitation, BG: Visible Excitation, IR: Near IR. "( )" indicates that fluorescence might be slightly darker.
***Special dedicated cover glass and immersion oil needed.
3-36
Microscope Frame
BX53M: Upright Transmitted and Reflected Light Microscope Frame
BX53MTRF-S
Designed with modularity in mind, the BX3M series provide versatility for a wide variety of material science and industrial applications.
The frames are outfitted with electro-static discharge (ESD) capability to help protect electronic samples.
81.5
192
290.5
BX53MTRF-S
90
91.4
274.4
362.2
Weight: 7.6 kg
4-1
Unit: mm
Microscope Frame
BX53M: Upright Reflected Light Microscope Frame
BX53MRF-S
Designed with modularity in mind, the BX3M series provide versatility for a wide variety of material science and industrial applications.
The frames are outfitted with ESD capability to help protect electronic samples.
81.5
192
290.5
BX53MRF-S
90
91.4
274.4
362.2
Weight: 7.4 kg
4-2
Unit: mm
Microscope Frame
BX3: Automated Transmitted Light Microscope Frame
BX63F
This fully motorized system enables automation of complex multidimensional experiments and features a precise motorized Z-drive and
splendid stability thanks to the fixed stage design.
90
359
BX63F
110
152.5
294.4
429.2
Weight: 14.1 kg
4-3
Unit: mm
Microscope Frame
BX3: Semi-Motorized Fluorescence Transmitted Light Microscope Frame
BX53F
The entire optical path of the BX53 is designed for optimal fluorescence imaging and uses UIS2 optical components that set a new
standard in precision and clarity. The modular concept enables motorization of individual components.
97
319
BX53F
90
91.4
274.4
362.2
Weight: 8.3 kg
4-4
Unit: mm
Microscope Frame
BX3: Manual System Transmitted Light Microscope Frame
BX43F
The BX43 microscope offers a wide range of features, splendid optical performance, and is the ideal platform for digital imaging. This
flexible microscope offers various contrast methods and leading-edge optics combined with true-color LED illumination for true-to-life
color rendering.
81.5
192
319
BX43F
90
91.4
274.4
362.2
Weight: 9.1 kg
4-5
Unit: mm
Microscope Frame
BX3: Transmitted Light Ergonomic Microscope Frame
BX46F
The BX46 features an ergonomic design with a low-position fixed stage and nosepiece focus that helps keep users comfortable while
they're working.
81.5
319.8
112
64
143.8
BX46F
90
91.4
274.4
362.2
Weight: 9.8 kg
4-6
Unit: mm
Microscope Frame
BX2: Upright Motorized Transmitted/Reflected Frame
BX61TRF
The BX61 frame is designed to incorporate a laser-based autofocus unit for active focus tracking, making inspections faster and highly
reproducible.
84
319
BX61TRF
90
70
317.5
341.2
Weight: 11.4 kg
4-7
Unit: mm
Microscope System BXFM
BXFM Frame
BXFM-F
This popular microscope frame can be used with fiber illumination, a motorized nosepiece, and a telan lens unit. The frame can easily
be integrated into other equipment, which can be attached by a rear bolt mounting screw or pillar mounting hole.
84
36
82
4–M4 Depth 9
(13)
Stroke
35
0.5
0.5
4–M4 Depth 7
66.2
16
17
Pillar Mount Hole Center
(13)
17
80
55
124
100
17
34
23
4-M8 Depth 8
Bolt Mount Screw
7
58.5
36
2A00002
ø3
2H
Pil 8
lar
Mo
un
98
tH
ole
110
Weight: 1.9kg
4-8
Unit: mm
Microscope System BXFM
BXFM System Configuration Example 1
BXFM-F + BXFM-ILH + BXFM-ILHSPU
180
This example accommodates reflected light brightfield/darkfield and fluorescence illuminators.
Light Axis
165
Pillar Axis
130
Holder Mounting Position
83
3.5
Light Axis
Objective Mounting Position
11
45
72
40
Revolving Nosepiece Mounting Position
Specimen Position
Weight: 3.2kg
Unit: mm
BXFM Combination Sample
BXFM-F + BXFM-ILH + BXFM-ILHSPU + TR30-2 + BX-RLA2 + U-LH100L-3
165
130
87.5
83
17-47 Stroke
124
45
11
40
3.5
249
ø 32
220
Specimen
Surface
180
587
Weight: 8.2kg (excludes objectives)
*For installation dimensions, refer to the BXFM-F diagram.
4-9
Unit: mm
Microscope System BXFM
BXFM System Configuration Example 2
BXFM-F + BXFM-ILHS
84
This system example contains a compact focusing unit that is suitable for being integrated into existing equipment.
141
106
Holder Mounting Position
Light Axis
23
Revolving Nosepiece
Mounting Position
40
20
7
59
Stroke
Pillar Axis
45
Objective
Mounting Position
Specimen Position
Weight: 2.4kg
Unit: mm
BXFM-S Combination Sample
BXFM-F + BXFM-ILHS + TR30-2 + U-KMAS + U-LH100L-3
290
187
92.5
106
19-49
Stroke
169
20
45
40
124
ø32
Specimen Surface
84
208
Weight: 5.5 kg (excludes objectives)
*For installation dimensions, refer to the BXFM-F diagram.
4-10
Unit: mm
Microscope System BXFM
Stands for the BXFM
A wide variety of stands are available to suit different applications and purposes.
SZ2-STU2
Universal Stand Type 2
Major Specifications
Item
Specifications
250
300
Diameter of Focusing Arm or
1
Fixing Section of Tube
300
350
622
2 Vertical Pole Diameter
ø40 mm
3 Diameter of Horizontal Poles
ø25 mm
(both upper and lower poles)
4 Stroke
Horizontal: 234 mm
Vertical: 205 mm
5 Movement Range
Horizontal: 541 (435 + 106) mm max.
(vertical pole — BXFM-S optical
axis)
6 Maximum Specimen Weight
Forward: 10 kg
(within 90-degree area)
Transverse Direction: 6 kg
Backward Direction: 7 kg
(at maximum stroke)
7 Weight
30 kg
º
90
ø25
180º
214~435
30
503
Ø32
ø32 mm
ø40
50.5
130
*The rotation angle of the horizontal arm can be restricted to 90 degrees with a stopper.
SZ-STL
Large Stand
320
133
U-ST
Compact Stand
88
160
ø110
35
106
73
143
400
ø32
267
17.5
45
46
20
49
133
ø32
Weight: 1.8 kg
Weight: 5 kg
Unit: mm
4-11
Illumination Units
Reflected Light Illuminators for the BX53M Microscope
Manual illuminators for brightfield, darkfield, and fluorescence applications.
BX3M-RLA-S
Reflected Light Illuminator for BF/DF for the BX53M Microscope
Accessories
Weight (g)
Green Filter
20
U-25L42
UV-Cut Filter
20
U-25Y48
Yellow Filter
20
U-25FR
Frost Filter
20
U-25
Empty Filter (for use with user's
ø25 mm filters)
<20
U-25ND50
ND Filter
20
U-25ND25
ND Filter
20
U-25ND6
ND Filter
20
ø75
88
20
U-25IF550
29.4
38.7
45
3.5
Revolving
Nosepiece
Mounting
Position
56.8
41
Halogen Color Filter
84
20
U-25LBA
127
(108)
Description
Daylight Color Filter
54
Unit Name
U-25LBD
Illuminator
Mounting
Position
265
327.5
338.1
Weight: 3.0 kg
BX3M-KMA-S
BF Reflected LED Light Illuminator for the BX53M Microscope
Accessories
Fixed Polarizer
71
U-POTP3
Fixed Polarizer (use with U-DICRH)
71
U-25LBD
Daylight Color Filter
20
U-25LBA
Halogen Color Filter
20
U-25IF550
Green Filter
20
U-25L42
UV-Cut Filter
20
U-25Y48
Yellow Filter
20
U-25FR
Frost Filter
20
U-25
Empty Filter (for use with user's
ø25 mm filters)
<20
U-25ND50
ND Filter
20
U-25ND25
ND Filter
20
U-25ND6
ND Filter
20
88
U-PO3
54
79
16
Revolving
Nosepiece
Mounting
Position
41
Rotatable Analyzer
108
50
U-AN360-3
ø75
Weight (g)
84
Description
Fixed Analyzer
3.5
Unit Name
U-AN-2
Illuminator
Mounting
Position
236
298.5
Weight: 2.4 kg
Unit: mm
5-1
Illumination Units
Coded Reflected Light Illuminator for the BX53M Frame
Coded illuminators require users to physically change the device's settings, but the microscope's software automatically recognizes
these changes.
BX3M-RLAS-S
Coded Reflected Light Illuminator for BF/DF for the BX53M Frame
Accessories
U-PO3
Fixed Polarizer
71
U-POTP3
Fixed Polarizer (use with U-DICRH)
71
U-25LBD
Daylight Color Filter
20
U-25LBA
Halogen Color Filter
20
U-25IF550
Green Filter
20
U-25L42
UV-Cut Filter
20
U-25Y48
Yellow Filter
20
U-25FR
Frost Filter
20
U-25
Empty Filter (for use with user's
ø25 mm filters)
<20
U-25ND50
ND Filter
20
U-25ND25
ND Filter
20
U-25ND6
ND Filter
20
161.4
(150)
79
75
Rotatable Analyzer
89.1
Revolving
Nosepiece
Mounting
Position
37.4
38
50
U-AN360-3
ø78.8
88
Weight (g)
84
Description
Fixed Analyzer
3.5
Unit Name
U-AN-2
Illuminator
Mounting
Position
64
285
394.7
Weight: 3.6 kg
BX3M-URAS-S
Coded Universal Reflected Illuminator for the BX53M Frame
Accessories
Fixed Polarizer
71
U-POTP3
Fixed Polarizer (use with U-DICRH)
71
U-25LBD
Daylight Color Filter
20
U-25LBA
Halogen Color Filter
20
U-25IF550
Green Filter
20
U-25L42
UV-Cut Filter
20
U-25Y48
Yellow Filter
20
U-25FR
Frost Filter
20
U-25
Empty Filter (for use with user's
ø25 mm filters)
<20
U-25ND50
ND Filter
20
U-25ND25
ND Filter
20
U-25ND6
ND Filter
20
88
U-PO3
ø84
79
75
Rotatable Analyzer
87.1
Revolving
Nosepiece
Mounting
Position
26.8
38
50
U-AN360-3
151.2
(150)
Weight (g)
84
Description
Fixed Analyzer
3.5
Unit Name
U-AN-2
Illuminator
Mounting
Position
40
261
371
Weight: 3.2 kg
Unit: mm
5-2
Illumination Units
Reflected Illuminator for the BX3 Series
Manual illuminators for brightfield, darkfield, and fluorescence applications.
BX3-URA
Universal Reflected Illuminator for the BX3 Series
A total of eight fluorescence mirror units can be attached for multi color fluorescence observations.
Accessories
Fixed polarizer
71
U-POTP3
Fixed polarizer (use with U-DICRH)
71
U-25LBD
Daylight color filter
20
U-25LBA
Halogen color filter
20
U-25IF550
Green filter
20
U-25L42
UV-cut filter
20
U-25Y48
Yellow filter
20
U-25FR
Frost filter
20
U-25
Empty filter, for use with user's ø25
mm filters
<20
U-25ND50
ND filter
20
U-25ND25
ND filter
20
U-25ND6
ND filter
20
88
U-PO3
ø84
79
90
Rotatable analyzer
86.2
Revolving
Nosepiece
Mounting
Position
28.9
3.5
50
U-AN360-3
(180)
Weight (g)
38
Description
Fixed analyzer
84
Unit Name
U-AN-2
40
Illuminator
Mounting
Position
261
402.5
Weight: 3.8 kg
BX3-RFAS
Coded Fluorescence Illuminator for the BX3 Series
The eight fluorescence mirror units feature coded functionality.
Accessories
Fixed polarizer
71
U-POTP3
Fixed polarizer (use with U-DICRH)
71
U-25LBD
Daylight color filter
20
U-25LBA
Halogen color filter
20
U-25IF550
Green filter
20
U-25L42
UV-cut filter
20
U-25Y48
Yellow filter
20
U-25FR
Frost filter
20
U-25
Empty filter, for use with user's ø25
mm filters
<20
U-25ND50
ND filter
20
U-25ND25
ND filter
20
U-25ND6
ND filter
20
88
U-PO3
ø84
79
90
Rotatable analyzer
86.2
Revolving
Nosepiece
Mounting
Position
42.8
38
50
U-AN360-3
(180)
Weight (g)
84
Description
Fixed analyzer
3.5
Unit Name
U-AN-2
40
Illuminator
Mounting
Position
261
402.5
Weight: 3.9 kg
Unit: mm
5-3
Illumination Units
Reflected Light Illuminator for the BX2 Series
BX-RLA2
Reflected Light Illuminator for BF/DF
Neutral density (ND) filters are linked when exchanging between brightfield and darkfield.
144
Weight (g)
71
U-POTP3
Fixed Polarizer (use with U-DICRH)
71
U-AN360-3
Rotatable Analyzer
79
U-AN
Analyzer Slider for Reflected Light
50
U-DICR
DIC Slider for Reflected Light
130
U-DICRH
DIC Slider for Reflected Light
(Resolution Type)
130
U-DICRHC
DIC Slider for Reflected Light
(Contrast Type)
130
107.5
Fixed Polarizer
35
20
U-PO3
17.5
UV-Cut Filter
ø75
88
20
U-25L42
7.5
16.2
20
Frost Filter
29.4
Revolving
Nosepiece
Mounting
Position
Illuminator
Mounting
Position
56.8
13
ND Filter
U-25FR
12
U-25ND25
11.8
20
17
ND Filter
26
20
U-25ND6
46
20
108
Green Filter
84
U-25IF550
38.7
41
Daylight Color Filter
30
Description
U-25LBD
3.5
Unit Name
31.5
30
Accessories
45
265
335
Weight: 3.4 kg
BX-URA2
Universal Reflected Light Illuminator
This illuminator is optimized for observations ranging from brightfield to fluorescence.
Six mirror units can be simultaneously attached to this reflected light illuminator.
Accessories
Weight (g)
20
20
U-25L42
UV-Cut Filter
20
U-PO3
Fixed Polarizer
71
U-POTP3
Fixed Polarizer (use with U-DICRH)
71
U-AN360-3
Rotatable Analyzer
79
U-AN
Analyzer Slider for Reflected Light
50
U-DICR
130
Mirror Unit for Reflected Darkfield
U-MDIC3
Mirror Unit for Reflected DIC
Mirror Unit for Reflected Brightfield,
for a High Intensity Light Source
Fluorescence Mirror Unit for
Reflected (U Excitation)
Fluorescence Mirror Unit for
Reflected (B Excitation)
Fluorescence Mirror Unit for
Reflected (G Excitation)
U-DICRHC
U-MBFL3
U-MWUS3
U-MWBS3
U-MWGS3
130
84
U-MDF3*
U-DICRH
130
80
80
80
Revolving
Nosepiece
Mounting
Position
86.8
3.5
U-MBF3
DIC Slider for Reflected Light
DIC Slider for Reflected Light
(Resolution Type)
DIC Slider for Reflected Light
(Contrast Type)
Mirror Unit for Reflected Brightfield
ø84
88
20
Frost Filter
12
ND Filter
U-25FR
Illuminator
Mounting
Position
11.6
U-25ND25
17
20
11.6
ND Filter
27.2
41
20
U-25ND6
(152)
Green filter
76
U-25IF550
.9)
Description
Daylight Color Filter
(17
Unit Name
U-25LBD
5
41
13
261
367
80
Weight: 3.8 kg
80
80
80
Unit: mm
5-4
Illumination Units
Mounting Dimensions of Illuminator (BX3M-RLA-S, BX3M-RLAS-S, BX3M-URAS-S, BX3M-KMA-S, BX3-RFAS, BX3-URA)
174 Distance to the Light Axis
144
100±0.1
32±0.1
45°
Location
Face
R6
0
62±0.1
凸3.5
37± 0
45 Location Face
2
R6
48
Revolving Nosepiece Relief Dimension
4-M5 Depth 12 or more
47.3
(6)
Location Face
89.2
Revolving Nosepiece Relief Dimension
Fix illuminator using four M5 screws and projection for fastening.
Unit: mm
Mounting Dimensions of Illuminators (BX-RLA2 and BX-URA2)
100 (0.1)
21
36 (0.1)
170
Distance to the Light Axis
2
5.5
4-M5 Depth 12 or more
(6)
(11)
Location Face
37 0.1
45 Location Face
45 (1
0')
2.5
62 (0.1)
5
52
Revolving Nosepiece Relief Dimension
3.5
Location Face
ss
Le
an
th
82
Revolving Nosepiece Relief Dimension
R6
Convex Section (2-4) for Positioning
Unit: mm
Fix illuminator using four M5 screws and projection for fastening.
5-5
Illumination Units
Compact Reflected Light Illuminator for BF
U-KMAS
Very compact reflected light illuminator with reduced depth.
U-KMAS
Accessories
Weight (g)
ND Filter
20
U-25ND25
ND Filter
20
U-25FR
Frost Filter
20
U-25L42
UV-Cut Filter
20
U-PO3
Fixed Polarizer
71
U-POTP3
Fixed Polarizer (use with U-DICRH)
71
U-AN360-3
Rotatable Analyzer
79
U-AN
Analyzer Slider for Reflected Light
50
U-DICR
DIC Slider for Reflected Light
DIC Slider for Reflected Light
(Resolution Type)
DIC Slider for Reflected Light
(Contrast Type)
130
U-DICRHC
ø75
20
U-25ND6
U-DICRH
20
88
Green Filter
64
U-25IF550
21
Description
Daylight Color Filter
6
Unit Name
U-25LBD
130
155
21
198
130
Weight: 1.2 kg
Unit: mm
5-6
Light Source Units
LED Lamp Housing for the BX53M Microscope
The BX3M microscope utilizes a high-intensity white LED light source for both reflected and transmitted light. High-intensity light
support various observation modes such as BF, DF, DIC, and polarizing.
BX3M-LEDR
ø59
ø79
LED Lamp Housing for BX3M Reflected
52
63.5
Weight: 0.5 kg
BX3M-LEDT
ø59
ø68
LED Lamp Housing for BX3M Transmitted
65.5
77
Weight: 0.5 kg
BX3M-PSLED
82
85
Power Supply for LED Lamp House (required with the BXFM)
42.6
111
124.2
Weight: 0.36 kg
Unit: mm
6-1
Light Source Units
LED Lamp Housing for the BX3 Series
The BX3M microscope utilizes a high-intensity white LED light source for both reflected and transmitted light. High-intensity light
support various observation modes such as BF, DF, DIC, and polarizing.
U-LHLEDC
LED Lamp Housing for BX3M Transmitted
8
57
ø48
ø54
ø6
16.5
41.5
86.5
Weight: 0.5 kg
U-LEDPS
External Power Supply for U-LHLEDC
The BX3 utilizes LED illumination that provides true color reproduction and an optimized
color temperature, enabling consistent color reproduction for brightfield stains.
45
48
185
120
129.5
Weight: 0.78 kg
Unit: mm
6-2
Light Source Units
Lamp Housings
A variety of different lamp housings are available for use with different light sources, enabling users to choose the most appropriate
housing for their application.
For customers who use these units in a production line, please consult your nearest Olympus representative to discuss your specific needs.
U-LH75XEAPO
U-LH100HGAPO
75 W Xenon Apo Lamp Housing
100 W Mercury Apo Lamp Housing
U-LH100HG
100 W Mercury Lamp Housing
(148.5)
(148.5)
30.2
83.5
65
65
65
93
(30.2)
83.5
65
93
8
8
130
115
75
75
55
6 20
20
40.8
40.8
115
30
25
30
25
130
169 (depth dimension for installation)
70
169 (depth dimension for installation)
18.5
180.5
180.5
*Power supply unit (U-RX-T) and power cable (UYCP) are required for the
75 W xenon lamp housing. These items are sold separately.
Cable Length : 2,000 mm
Accepted Lamp: USH-103OL
*Power supply unit (U-RFL-T) and power cable (UYCP) are required for the
100 W mercury lamp housings. These items are sold separately.
Weight: 2.7 kg
Cable Length : 2,000 mm
Accepted Lamp: USH-103OL
Weight: 2.7 kg
U-RFL-T
Power Supply Unit for Mercury Lamp
U-RX-T
Power Supply Unit for Xenon Lamp
90 1
58.2
31.2
115
31
186±1
180±1
53
150
122
65.7
31
2701
Weight: Approximately 3 kg
Unit: mm
6-3
Light Source Units
Halogen Illumination
The external power supply for the 100 W halogen lamp (TH4-100/200) features an intensity adjustment knob and an on/off switch, both
located close to the operator’s hand for splendid comfort.
For customers who use these units in a production line, please consult your nearest Olympus representative to discuss your specific needs.
Illumination devices for microscopes have suggested lifetimes. Periodic inspections are required. Please visit our website for details.
U-LH100-3/U-LH100IR/U-LH100L-3
85.5
107
100 W Halogen Lamp Housings
Cable Length U-LH100-3: 290 mm
U-LH100IR: 290 mm
U-LH100L-3: 800 mm
37
Accepted Lamp: 12V100WHAL (high-intensity lamp)
12V100WHAL-L (long-life lamp)
*External power supply (TH4-100 or TH4-200) and
power cable (UYCP) are required for 100 W halogen
lamp housings. These items are sold separately.
135
Depth Dimension for Installation
146.5
Weight: 880 g
TH4-100/200
TH4-HS
External Power Supply
Hand Switch
Cable Length:
2,000 mm
77
120
125
18.5
75
14.5
200
42
Weight: 2.2 kg
38
Weight: 140 g
U-RMT
Extension Cord
1,700
Weight: 200 g
Unit: mm
6-4
Light Source Units
Halogen Fiber Illumination Accessories
All Olympus reflected light illuminators can be used with fiber illumination.
For customers who use these units in a production line, please consult your nearest Olympus representative to discuss your specific needs.
Illumination devices for microscopes have suggested lifetimes. Periodic inspections are required. Please visit our website for details.
37.5
ø32
ø59
ø59
ø75
ø67
U-LGAD
Fiber Adaptor for Reflected Light Observation
43
U-RCV
DF Converter for BX-URA2
21
51
31.5
Tube to exclude darkfield light
(built into the BX-URA2).
(42.5)
Dimensions
for installation
62.5
Weight: 315 g
Dimension
for installation
Light Guide Mount Hole ø12;
Weight: 390 g
LG-PS2*
Light Source
ø15
Light Guide Mounting Position
8
235
10
86
130
76
10
126
251
*The types of models in use varies by country.
Weight: 1.6 kg
LG-SF
Light Guide
Groove: Width 3, Depth 1
61
ø13
ø25
30
31
ø10.1
ø12
ø15
10
25
20
1,000
Weight: 210 g
Unit: mm
6-5
Light Source Units
Lamp Housing Accessory
Two lamp housings can be attached simultaneously.
For customers who use these units in a production line, please consult your nearest Olympus representative to discuss your specific needs.
U-DULHA
Double Lamp House Adaptor
82
88
202
ø14
171
0
Weight: 1.2 kg
Unit: mm
6-6
Condenser Units
Universal Condenser
U-UCD8-2
The universal condenser contains 8 optical element slots.
*Optical elements are required. Please consult your local Olympus representative for information about different optical element combinations.
128.5
131
44.1
67.6
U-UCD8-2
112
149.8
Weight: 700 g
Unit: mm
7-1
Condenser Units
Condenser
Condensers for different applications: brightfield, darkfield, and long working distance.
17.2
79.8
51.8
59.8
42.8
50.3
25.3
41.8
ø36
ø16
43.3
ø62
ø38
34.3
ø62
4.3
U-LWCD
Long Working Distance Condenser
(0.119)
U-AC2-7
Abbe Condenser
ø47
ø62
ø68
ø47
ø62
ø68
Weight: 174 g
Weight: 380 g
WI-OBCD
Long Working Distance Oblique Condenser
ø66
47.5
ø8
56
(56.2)
26
48.1
34
41.4
34
(0.2)
28
ø24
10.5
ø45
45
16
30
ø47
ø66
Weight: 530 g
CH3-CD
Brightfield Condenser
ø46
ø41
44.3
26.3
41.8
5.4
25
ø28
13.8
(0.3)
ø36
6.8
ø36.8
5
40
ø44
ø61.5
Weight: 60 g
Unit: mm
7-2
Observation Tubes
Super Widefield Trinocular Observation Tubes
Trinocular observation tubes with a super-wide field of view. These tubes are compatible with objectives up to FN 26.5.
U-SWTR-3
14
55.6
79.6
98.8
82.3*
63.1
180.8
Super Widefield Trinocular Tube
173.8
220.8
68.6
49.9
MX-SWETTR
139.400
61.981
117.223
Super Widefield Erect Image Tilting Trinocular Tube
88.000
95.929
101.929
149.081
70.330
2.837
20 0' 0"
42 0
' 0"
318.527
328.338
337.718
72.200
Unit: mm
Name
U-SWTR-3
MX-SWETTR
FN
26.5
26.5
Inclination Angle
(degrees)
24
0–42
Interpupillary Distance Light Path Selector
(mm)
(eyepiece/video port)
50–76
100/0, 20/80, 0/100
50–76
100/0, 0/100
Observation Image
*Length marked with an asterisk (*) may vary according to interpupillary distance. The distance for the figure shown is 62 mm.
8-1
Inverted
Erect
Weight
(g)
2300
4200
Observation Tubes
Widefield Trinocular Observation Tubes
Trinocular observation tubes with wide field of view. These tubes are compatible with objectives up to FN 26.5.
U-TR30-2
U-ETR-4
Widefield Trinocular Tube
Widefield Erect Image Trinocular Tube
U-TR30IR
60.6
62.5
103.9
104.9
92
47.9
62.5 (IR: 64.5)
150.5
186.6 (IR: 188.9)
95.8
18
92.5* (IR: 93.9)
59.65
33.5
16
175
120
Widefield Trinocular Tube for IR
43.5
163.1
199.9
51.6
Unit: mm
Name
U-TR30-2
U-TR30IR
U-ETR-4
FN
22
22
22
Inclination Angle
(degrees)
30
30
30
Interpupillary Distance Light Path Selector
(mm)
(eyepiece/video port)
50–76
100/0, 20/80, 0/100
50–76
100/0, 0/100
50–76
100/0, 0/100
Observation Image
Inverted
Inverted
Erect
Weight
(g)
1600
1600
1900
*Length marked with an asterisk (*) may vary according to interpupillary distance. The distance for the figure shown is 62 mm.
Single Port Tube with Lens
When visual observation is not needed and only video observation is required, a single port tube with a built-in telan lens can be
attached directly to the video port.
U-TLU
Single Port Tube with Lens
U-TLUIR
Single Port Tube with Lens for IR
57.6
ø60
• For attachable video camera adaptors, refer to the video camera adaptors system diagram (pages 2-12 and 2-13).
8-2
Weight: 350 g
Unit: mm
Observation Tubes
U-TTR-2
Tilting Trinocular Tube
220
62
118.3
236
130
U-TTBI
Telescopic Tilting Binocular Tube
62
110.8
Tilting Binocular Tube
45
149.5
66
167.7
40.8
178.6
201.2
203
219.5
45
160
142.5
82
5º
104.4
180
178
35º
234.5
25º
19
317.2
218
U-TBI-3
136.2
40.1
16
22
5°–35°
62
111
Tilting Binocular Tube
Unit: mm
Name
U-TTBI
U-TTR-2
U-TBI-3
FN
22
22
22
Inclination Angle
(degrees)
0–25
5–35
5–35
Interpupillary Distance Light Path Selecter
(mm)
(eyepiece/video port)
50–76
NA
50–76
100/0, 0/100, 50/50
50–76
NA
*When combined with the CX-RFA-2, the field number is reduced to 18.
8-3
Observation Image
Inverted
Inverted
Inverted
Weight
(g)
3800
3200
1300
Observation Tubes
Binocular Tube
U-CBI30-2
49.5
92.5
93.3
93.9
Binocular Tube
68.1
43.8
144.9
181.4
U-CTR30-2
49.5
51.3
92.5
106.2
94
Trinocular Tube
68.4
44
144.9
181.4
Unit: mm
Name
U-CBI30-2
U-CTR30-2
FN
20, 18(*)
20, 18(*)
Inclination Angle
(degrees)
30
30
Interpupillary Distance Light Path Selector
(mm)
(eyepiece/video port)
48–75
NA
48–75
NA
*When combined with the CX-RFA-2, the field number is reduced to 18.
8-4
Observation Image
Inverted
Inverted
Weight
(g)
800
900
Intermediate Tubes and Accessories
Intermediate Tubes
Olympus makes various accessories to satisfy a wide variety of observation needs.
U-CA
U-ECA
Magnification Changer
Magnification Changer 2C
Provides 1X, 1.2X, 1.6X, and 2X intermediate magnifications.
Provides 1X and 2X intermediate magnifications.
U-ECA1.6X
Magnification Changer 1.6X
Provides 1X and 1.6X intermediate magnifications.
150
ø138
ø140
88
42
37
96
52
45
42
ø75
ø70
Weight: 1.3 kg
Weight: 1.3 kg
U-TRU
Trinocular Intermediate Attachment
The intermediate attachment divides the light path,
enabling users to attach both digital and video cameras.
106.9
58.2
52
37
150
ø140
183.9
BI:PT=100:0/20:80
Weight: 1.3 kg
Unit: mm
9-1
Intermediate Tubes and Accessories
Intermediate Tubes
Olympus makes various accessories to satisfy a wide variety of observation needs.
U-DP
U-DP1XC
Dual Port
Dual Port 1X
Use this intermediate tube to divide the light path.
Combine this component with the U-DP to obtain a 1X image.
ø140
88
ø44
ø30
151
ø44
ø25
17.53
1-32UN
4.5
170.5
Mount Face
182
Weight: 500 g
57
38
51
Mount Face
Weight: 1 kg
Light path selector via mirror unit
Transmitted Side Port: Side Port = 100:0
Transmitted Side Port: Side Port = 70:30 (with use of U-MBF3)
U-EPA2
U-APT
Eyepoint Adjuster
Arrow Pointer
115
(8)
89
This component rases the eyepoint by 30 mm.
15V0.2A
0.92
Tolerance from Light Axis
120
Weight: Approximately 500 g
(45)
45
30
21.2
88
45.3
Weight: 1.2 kg
Unit: mm
9-2
Intermediate Tubes and Accessories
Dual port tube with C-mounts
U-DPCAD
The dual camera port enables the user to attach two cameras.
U-DPCAD
n45
n34
35
n
25.4
50
ø45
43
23
(94.5)
52.5
31.5
41.5
ø25.4
ø42.3
41.47
ø54
116.47
119
111
79
Weight: 0.9 kg
Dual port tube with C mounts
9-3
Unit: mm
Eyepieces
Eyepieces
Eyepieces for the UIS2 optical system.
WHN10X
WHN10X-H
CROSSWHN10X
Widefield Eyepiece
WH15X
Widefield Eyepiece
Widefield Eyepieces
ø41
ø41
ø41
ø38.5
ø38.5
ø36.5
36.8
18.7
29.6
48.6
27.8
39.4
(25)
43.7
(23.2)
(23.1)
62.6
51.2
28
39.6
48.6
EP
ø30
ø30
ø30
SWH10X-H
MICROSWH10X
CROSSSWH10X
Super Widefield Eyepieces
ø46.2
ø43.2
ø39
69.9
(28.5)
41.4
53.2
60.2
EP
ø30
unit: mm
Name
WHN10X
WHN10X-H
CROSSWHN10X
WH15X
SWH10X-H
MICROSWH10X
CROSSSWH10X
FN
22
22
22
14
26.5
26.5
26.5
Diopter Adjustment Range Micrometer Diameter
(1/m)
(mm)
—
-8–+5
-8–+5
—
-8–+2
-8–+2
-8–+2
24
24
—
24
—
—
—
*EP=eyepoint
10-1
Weight
(g)
90
170
170
90
210
210
210
Remarks
With adjustable diopter
With cross lines and adjustable diopter
With adjustable diopter
With micrometer and adjustable diopter
With cross lines and adjustable diopter
Revolving Nosepieces
Revolving Nosepieces for BF Objectives
Users can choose from the 6 revolving nosepieces for BF objectives shown below. For motorized nosepieces, please refer to the
motorized unit page.
U-D6RE-ESD
ESD-Resistant Sextuple Revolving
Nosepiece with Slider Slot for DIC
104
(87.4)
(114.1)
(114.4)
48.2
48.2
.4
ø102
ø84
Weight: 520 g
76.1
ø102
Weight: 800 g
Weight: 0.7 kg
U-P6RE
Centerable Sextuple Revolving
Nosepiece with Slider Slot for DIC
104
116.5
U-P4RE
Centerable Quadruple Revolving
Nosepiece with Slider Slot for DIC
116.5
U-D7RE
Sextuple Revolving Nosepiece
with Slider Slot for DIC
(125.6)
(114.4)
(125.6)
76.4
47.2
38
40
48.2
38
.4
ø102
ø116.5
Weight: 980 g
87.6
40
87.6
40
38
47.2
38
76.4
40
38
40
60.9
40
40.8
26.5
104
U-D6RE
Septuple Revolving Nosepiece
with Slider Slot for DIC
83
U-5RE-2
Quintuple Revolving Nosepiece
.5
ø116
Weight: 1 kg
Weight: 1 kg
Insert the DIC dummy when not using the DIC slider.
11-1
unit: mm
Revolving Nosepieces
Revolving Nosepieces for BF/DF Objectives
Users can choose from the following 3 types of revolving nosepieces for BF/DF objectives. To attach brightfield objectives, users must
use the BF adaptor (BD-M-AD). For motorized nosepieces, please refer to the motorized unit page.
U-5BDRE
U-D5BDRE
U-P5BDRE
Quintuple Revolving Nosepiece for BF/DF
Quintuple Revolving Nosepiece for
BF/DF with Slider Slot for DIC
Centerable Quintuple Revolving
Nosepiece with Slider Slot for DIC
U-D6BDRE
116.5
104
2A00002
JAPAN
U-D6BDRE
104
Sextuple Revolving Nosepiece for
BF/DF with Slider Slot for DIC
N
PA
JA
(125.6)
(114.4)
47.2
48.2
38
87.6
76.4
40
38
.4
40
(111.2)
76.4
40
.4
ø102
ø102
Weight: 800 g
.5
Ø116
Weight: 800 g
Weight: 1 kg
Insert the DIC dummy when not using the DIC slider.
BD-M-AD
Adaptor to Mount BF Objectives
W 26 mm × 0.706 mm (1 in. × 0.03 in.)
ø28.2
+0.2
0
W 20.32 mm × 0.706 mm (0.8 in. × 0.03 in.)
ø30
48.2
34.8
(4)
4
8
Weight: 10 kg
unit: mm
11-2
Revolving Nosepieces
Coded Sextuple Revolving Nosepiece
Coded nosepieces for BF, DF, and DIC applications.
U-D6BDRES-S
U-D5BDRES-ESD
Coded Sextuple BF/DF Revolving
Nosepiece with Slider Slot for DIC
ESD-Resistant Coded Quintuple BF/DF
Revolving Nosepiece with Slider Slot for
DIC
ø104
ø104
116.4
U-D6RES
Coded Sextuple Revolving Nosepiece
with Slider Slot for DIC
34
34
34
(134.5)
(126.1)
88.1
87.5
38
38
48.5
47.4
86.4
Weight: 0.7 kg
Weight: 0.9 kg
76.1
40
76
40
Weight: 0.75 kg
U-D7RES
116.4
Coded Septuple Nosepiece with Slider Slot for DIC
(134.5)
96.5
87.6
40
38
47.2
48.2
38.1
(126.1)
96.5
88
ø116.4
unit: mm
11-3
Video Camera Adaptors
C-Mount Video Camera Ports
Enables users to directly attach a C-mount video camera. Five types are provided: 1X, 0.63X, 0.5X, 0.35X and 0.25X. All models feature
a focus adjustment function.
U-TV0.25XC
U-TV0.35XC-2
U-TV0.5XC-3
C-Mount Video Port with 0.25X Lens
C-Mount Video Port with 0.35X Lens
C-Mount Video Port with 0.5X Lens
3.5
1–32 UN
147.3
ø36
156.84
17.53
Image Plane
1–32 UN
1–32 UN
3.5
ø30
ø60
ø60
Weight: 1.2 kg
Weight: 100 g
U-TV1XC
C-Mount Video Port with 1X Lens
Image Plane
84.5
3.5
68.75
78.25
SN
94.5
17.5
Image Plane
17.53
Weight: 200 g
4
U-TV0.63XC
C-Mount Video Port with 0.63X Lens
1–32 UN
32.6
ø60
ø64
ø30
42.1
12.4
22.4
30.1
4
17.53
Image Plane
17.53
Image Plane
3G00707
TOKYO, JAPAN
2 A0 0 0 0 1
ø66.5
ø60
Weight: 430 g
Weight: 300 g
Field of View (FN)
2/3" CCD
1/2" CCD
Video Camera Adaptor
Projection
(projection lens)
Magnification
Projection Area (FN)
2/3" CCD
1/2" CCD
1/3" CCD
U-TV1X-2
1X
11
8
6
U-TV1XC
1X
11
8
6
U-TV0.63XC
0.63X
17.5
12.7
9.5
U-TV0.5XC-3
0.5X
22
16
12
U-TV0.35XC-2
0.35X
—
22
17.1
U-TV0.25XC
0.25X
—
—
24
Practical Field of View (mm) =
Projection Area (FN)
Objective Magnification
Projection Area
Adjust the focus on the video camera adaptor to help keep the eyepiece image in focus when switching magnification.
Typically, the video camera adaptor is focused by switching to a low magnification after focusing at a high magnification.
Unit: mm
12-1
Video Camera Adaptors
Video Camera Mount Adaptors
These video camera mount adaptors enable users to attach cameras with C, bayonet, Sony, and F-mounts. Use these adaptors with
the U-TV1X-2. Focus by amount of screwing into U-TV1X-2.
U-CMAD3
U-BMAD
U-SMAD
C-Mount Adaptor
Bayonet Mount Adaptor
Sony Mount Adaptor
Image Plane
Image Plane
ø44.5
ø30
1-32UN
M56X2
ø64.4
ø48
40
60
3
30
M56X2
49
60.5
80.5
20
48.7
ø42
4
48
38
4
17.53
Image
Plane
M56X2
ø64
ø64
Weight: 165 g
Weight: 80 g
U-TMAD
U-FMT
T-Mount Adaptor
F/T-Mount Adaptor *
Weight: 90 g
Image Plane
46.5
55
Image Plane
M42X0.75
ø45.7
23
43
13
4
ø42
ø54.7
M56X2
*This adaptor must be combined with U-TMAD.
ø64
Weight: 165 g
Weight: 80 g
Video Camera Port
This port can be attached directly to the trinocular observation tube as well as to the single port tube with lens.
U-TV1X-2
Video Port 1X
28
22
ø64
ø60
Weight: 80 g
Unit: mm
12-2
Motorized Units
Control Box for the BX53M/BXFM Microscope
BX3M-CB
Control Box for the BXFM Microscope
The control box can be connected to a PC using an RS232 or USB2.0 cable (attached to the microscope frame).
105.8
*Please consult your local Olympus representative for detailed system configuration and combination information.
115
55.8
66
Weight: 0.49 kg
BX3M-CBFM
Control Box for the BX53M Frame
The control box can be connected to a PC using an RS232 or USB2.0 cable (attached to the microscope frame).
109.2
*Please consult your local Olympus representative for detailed system configuration and combination information.
116
58.1
Weight: 0.67 kg
Unit: mm
13-1
Motorized Units
Motorized Universal Reflected Illminator for the BX2 Series
These various motorized units are used to help automate equipment.
BX-RLAA + U-D6REMC + U-LH100-3
Motorized BF/DF Reflected Light Illuminator + Motorized Nomarski DIC Sextuple Revolving Nosepiece + 100 W Halogen Lamp Housing
86.2
11.8
12
115
108
107
80
These components motorize the exchange of objectives, the selection of brightfield and darkfield observation, and the opening and closing of the
aperture diaphragm. The BX-UCB control unit has an RS232C connector, enabling control via a PC. For instructions on how to attach an illuminator,
please refer to the mounting directions for the BX-RLA2.
(487)
400
64.8
45
81.5
40
(169)
41
211.5
84
87
Illuminator Cable Length: 1,800 mm
Weight: 5.5 kg (excludes objectives)
BX-RFAA
Motorized Universal Reflected Light Illuminator
88
11.6
87.5
27.2
41
Revolving
Nosepiece
Mounting
Position
126
109.5
86.8
11.6
17
12
76
ø84
14
92
15
This reflected light fluorescence illuminator enables users to simultaneously attach six mirror units and incorporates a motorized mirror unit changer
and shutter.
Illuminator
Mounting
Position
135
41
261
371
Illuminator Cable Length: 1.800 mm
Weight: 4.3 kg
Unit: mm
13-2
Motorized Units
Motorized Units
These various motorized units are used to help automate equipment.
U-P5BDREMC
Motorized Centerable Quintuple BD Revolving Nosepiece with Slider Slot for DIC
U-D6BDREMC
Motorized Sextuple BD Revolving Nosepiece with Slider Slot for DIC
115.5
46
50
14
34
83.5
103.5
135
40
69.8
51.1
40.5
25.5
150
Weight: 80 g
U-D5BDREMC
Motorized Quintuple BD Revolving Nosepiece with Slider Slot for DIC
U-D6REMC
Motorized Sextuple Revolving Nosepiece with Slider Slot for DIC
U-P5REMC
Motorized Centerable Quintuple Revolving Nosepiece with Slider Slot for DIC
115
46
14
105
134
40
50.3
40.5
25.4
150
68.2
50
34
74
75
Unit: mm
13-3
Motorized Units
Control Box for the BX2 Series
These various motorized units are used to help automate equipment.
BX-UCB
U-HSTR2
Control Unit
Hand Switch
Motorized units, including the motorized illuminator and
auto focus unit, can be totally controlled from the BX-UCB.
105
212
216
108
32
7
146
125
310
332
Depth
*Extension cord U-RMT (1,700 mm) should be used to connect the lamp housing
(U-LH100-3) to the BX-UCB.
Weight: 1.0 kg
Cable Length 2,000 mm
Weight: 370 g
BX-REMCB
U-ACAD4515
Control Box for Motorized Nosepiece and BF/DF Illuminator
AC Adaptor for BX-REMCB
The BX-RLAA and U-D5BDREMC/U-D6REMC/U-P5REMC can be
controlled from the U-HSTR2 or directly from the computer
keyboard via an RS232C connector.
*BX-RFAA and U-D5BDREM/U-D6REM combination is not applicable.
2000 +100
0
39.8
34
711
144
190.4
351
129.51
Weight:
kg
Weight:
kg
Unit: mm
13-4
Motorized Units
Motorized Units for the BX2 Series
These various motorized units are used to help automate equipment.
U-AFA2M-VIS
Active Auto Focus Unit
This unit features a 785 nm auto focus laser light source. The multiple-spot sensor
enables fast, stable focusing on specimens with variable height differences.
*Class 1 laser product
108
313
45
51
62.5
*Consult your local Olympus dealer about the motorized focus.
Weight: 2.6 kg
Cable
AFA2M-CBL2M Weight: 0.23 kg Length: 2 m
AFA2M-CBL3M Weight: 0.36 kg Length: 3 m
U-FWR
Motorized Reflected Filter Wheel
With this filter wheel, quickly switch between 6 different filters.
180.5
24.5
58.5
30.5
42
130
147.9
2000
Weight: 1.0 kg
Unit: mm
13-5
Motorized Units
Motorized Modular Microscope
With these components, users can integrate motorized microscope units into their equipment. Motorized operations, such as moving
the nosepiece up and down, changing objectives, opening and closing the aperture diaphragm, and changing between brightfield and
darkfield observation modes, are made possible using these components. Several of these operations can be totally controlled from an
external unit by adding an auto focus (AF) unit.
BXFMA-F
Motorized Illumination with Power Focus
133
45
56
310.5
206
77
This configuration combines the active auto focus unit (U-AFA2M-VIS), a single port tube with lens (U-TLU), a lamp housing, a
motorized nosepiece, and objectives.
36
169
32.5
2
51
7
77
334
342.1
92
Weight: 13 kg (BXFMA-F frame 7.6 kg)
*Please consult your local Olympus representative for information about the mounting dimensions.
U-FH
U-IFFH
Focus Adjustment Knob Unit
Focus Adjustment Knob Interface
70
3
50
100
104
91.5
33.6
70
75.5
4
210
54
82.3
214
Weight: 760 g
Weight: 1450 g
Unit: mm
13-6
Motorized Units
Motorized Units for the BX3 Series
The flexibility of the motorized fluorescence illuminator accommodates multi color stained specimens. The 8-position mirror unit permits
quick changeover of fluorescence colors.
BX3-RFAA
88
90
ø84
(180)
Motorized Fluorescence Illuminator
86.2
42.8
40
261
402.5
Weight: 4.2 kg
BX3-UCD8A
U-D7REA
Motorized Universal Condenser
Motorized Septuple Revolving Nosepiece with Slider Slot for DIC
The motorized universal condenser integrates a variety of optical
elements to accommodate transmitted light techniques including
brightfield, DIC, and phase contrast observation.
*Optical elements are required. Please consult your local Olympus
representative about optical elements combinations.
ø121
47
50.8
155.6
34
84.4
96
40
44.4
42
72
57
91.5
142.1
174.4
178
Weight: 1.6 kg
Weight: 1.5 kg
Unit: mm
13-7
Motorized Units
Control Box for the BX3 Series
BX3-CBH
Control Box for Fully-Motorized Function
210
214
The control box can be connected to a PC via the IEEE1394 cable.
100
280
288.8
Weight: 4.2 kg
U-CBM
Control Box M for Motorized Function
The control box can be connected to a PC via a RS232 cable.
45
185
120
125
Weight: 0.82 kg
U-CBS
Control Box for Coded Function
The control box can be connected to a PC via a RS232 cable (attached onto the microscope frame).
163
*Please consult your local Olympus representative for a detailed system chart.
117.5
48
*Please consult your local Olympus representative for a detailed system chart.
30.5
16.5
37
Weight: 0.5 kg
Unit: mm
13-8
Optical Terminology
1. FN and Practical Field of View
5. Total Magnification
The field number (FN) is the size (in mm) of the eyepiece
diaphragm, which defines the viewable area of a specimen.
The diameter on the sample plane that can actually be viewed
through the eyepiece is known as the practical field of view
(FOV) and is determined by the following formula:
5.1 Observation Through Eyepiece (binocular observation)
FOV =
M(bino)=M(ob)×M(oc)
M(bino): Total magnification for binocular observation
M(ob): Objective magnification
M(oc): Eyepiece magnification
Eyepiece FN
(mm)
Objective Magnification
5.2 Video Monitor Observation
Total Magnification for Video Monitor
M(video monitor)=M(ob)×M(video camera adaptor)×Monitor Magnification*
2. Working Distance
M(video monitor): Total Magnification on the Video Monitor
M(ob): Objective Magnification
M(video camera adaptor): Projected magnification for video camera
The working distance (W.D.) is the distance between the front
edge of the objective and the specimen surface (or the
surface of the cover glass when using a cover glass objective)
when the specimen is in focus.
adaptor including photo eyepiece
(refer to Figure 1)
*Refer to Figure 3 for "monitor magnification"
3. Parfocal Distance
Practical Field of View for Video Monitor Observation
The parfocal distance is the distance between the objective
mounting plane and the specimen. In UIS2/UIS objectives, the
parfocal distance is designed to be 45 mm.
Working Distance and Parfocal Distance
Practical Field of View for
=
Video Monitor Observation
Image Device Size *
M(ob)×M(video camera adaptor)
M(ob): Objective Magnification
M(video camera adaptor): Projected magnification for video camera
Objective
Mounting Position
adaptor including photo eyepiece
(refer to Figure 1 for projected magnifications)
*Refer to Figure 2 for image device size
Figure 1 Video Camera Adaptor and Projection Magnifications
Video Camera Adaptor (projection lens) Projection Magnification
U-TV1X-1 +
1X
Video Camera Mount Adaptors
U-TV1XC
1X
U-TV0.63XC
0.63X
U-TV0.5XC-3
0.5X
U-TV0.35XC-2
0.35X
U-TV0.25XC
0.25X
Parfocal Distance
W.D.
Focal Plane
For the parfocal distance of the LCPLFLN-LCD series
objectives, refer to the appropriate objective page.
Figure 2 Imaging Device Size
Camera Format
Diagonal
1/3"
6.0 mm
1/2"
8.0 mm
2/3"
11.0 mm
Horizontal
4.8 mm
6.4 mm
8.8 mm
Vertical
3.6 mm
4.8 mm
6.6 mm
The above table is for standard image device sizes.
Check your device size for precise calculation.
4. Relationship Between the Objective's Focal Length
and Magnification
Figure 3 Imaging Device Size and Monitor Magnifications
Monitor Size (diagonal)
Camera Format
10"
15"
17"
19"
42.3X
63.5X
72.0X
80.4X
1/3"
31.8X
47.6X
54.0X
60.3X
1/2"
23.1X
34.6X
39.3X
43.9X
2/3"
The magnification indicated for a UIS2/UIS objective is the
value when the focal length of the tube lens is 180 mm.
M(ob)= Focal Length of Tube Lens
21"
88.9X
66.7X
48.5X
Example
What is the total magnifications for a video monitor when a
objective is 50X, a video camera adaptor U-TV0.5XC, a 2/3"
video camera, and a 21" monitor are used ?
f
M(ob): Objective magnification
f: Objective's focal length
14-1
Optical Terminology
•Total magnification on the video monitor:
m(ob)=50X, M(video camera adaptor) is 0.5X from Figure 1, and the
monitor magnification is 48.5× from Figure 3.
M(monitor observation)=M(ob)×M(video camera adaptor)×monitor magnification
=50×0.5×48.5=1213X
Resolving Power Formula
The following formula is generally used for determing resolution.
λ
(Reyleigh formula)
NA
λ: Wavelength or radiation in use
(λ=0.55 μm is used for visible light.)
NA: Objective NA
ε =0.61×
•Practical filed of view for video observation (horizontal side):
M(ob)=50X, M(video camera adaptor) is 0.5X (from Figure 1), and
the horizontal side of a 2/3" imaging device is 8.8 mm (from
Figure 2)
Practical Field of View
for Video Observation
=
=
Example
MPLFLN100X (NA=0.90), λ=0.55 μm
Image Device Size
M(ob)×M(video camera adaptor)
ε = 0.61 ×
λ = 0.3355 = 0.3355 = 0.37 μm
NA
NA
0.90
8.8 (mm)
=52 μm
50 × 0.5
8. Focal Depth of Microscope
6. NA
The focal depth refers to the depth of the specimen layer that
is in sharp focus at the same time, even if the distance
between the objective and the specimen plane is changed
when observing and imaging the specimen plane using the
microscope. Because human eyes are individually different in
the ability to adjust their focus, each person's perception of
the focal depth varies.
At present, the Berek formula is generally used because it
gives a focal depth value that often coincides with the focal
depth obtained through experiments.
The numerical aperture is a key factor to the performance of
an objective (resolving power, focal depth, and brightness).
The NA is determined by the following formula:
NA= n × sinθ
n=The refraction rate of the medium between the specimen
and objective. (Air: n=1, oil: n=1.515)
θ: The angle made by the optical axis and refraction of the
light farthest from the center of the lens.
Focal Depth Formula
Visual Observation (Berek formula)
The visual field brightness (B) of the microscope is determined
by the following formula in relation to the objective
magnification (M). The larger the NA and the lower the
objective magnification, the brightness will increase by a factor
of the second power.
B∝
λ
± DOF= ω × 250,000 +
(μm)
NA × M
2 (NA) 2
DOF: Depth of Focus
ω: Resolving Power of Eyes 0.0014
(when the optical angle is 0.5 degrees)
M: Total Magnification
(objective magnification x eyepiece magnification)
NA2
M2
NA
➔ ± DOF =
Objective
n=1
(air)
350
0.275
+
(λ=0.55 μm)
NA × M
NA2
θ
This indicates that the focal depth becomes smaller as the
numerical aperture becomes larger.
Sample
Surface
Example
With MPLFLN100X (NA=0.90), WHN10X:
± DOF =
7. Resolving Power
The resolving power of an objective is measured by its ability
to differentiate two lines or points in an object. The greater the
resolving power, the smaller the minimum distance between
two lines or points that can still be distinguished. The larger
the NA, the higher the resolving power.
0.275
350
= 0.39 + 0.34 = 0.73 μm
+
0.90 × 1,000
0.81
Video Camera
In the case of a video camera, the focal depth will vary
according to the number of pixels of the CCD, optical
magnification, and numerical aperture. The above-mentioned
formula is used as a rough guide only.
14-2
Type (5) corresponds to “deformation” that goes against
requirement (iii) in Figure 9-1.
Types (6) and (7) correspond to the “color blur” of images
caused by characteristics of glass materials used for the
optical system. “Expansion of a point image” can also be
expressed by “wavefront aberration” that regards the light as
“waves” and takes into account the phase to include the
influence of diffraction.
9. Aberrations
A difference between an ideal image and the actual image that
passes through an optical system is called an “aberration.”
9.1 Requirements for Ideal Image Formation
The following three requirements must be satisfied to form an
image with no aberrations, or an ideal image.
(i) All the light rays coming from a single point and passing
through an image formation optical system converge on a
single point.
(ii) Image points, which correspond to object points on the
same plane perpendicular to the optical axis, are present
on the same plane.
(iii) The planar shape of an object and the planar shape of
an image that are on the same plane perpendicular to
the optical axis have a similar relation.
Figure 9-1
(1) Spherical Aberration
When light rays coming out of an axial object point enter a
lens, the light rays with a larger numerical aperture (NA) are
subjected to stronger refraction power and cross the optical
axis in positions further away from the ideal image formation
position. The aberration caused by different image forming
positions due to the differences in NA of axial light rays is
called “spherical aberration.” (“Spherical aberration” is
proportional to NA to the third power.)
Requirements for Ideal Image Formation
Figure 9-3
(i)
Object
Image Plane
(ii)
Spherical Aberration
(iii)
Aplanatic
Tube Lens
Specimen
Objective with
Spherical Aberration
In an actual optical system, however, it is very difficult to
strictly meet the requirements for ideal image formation, and
this causes “aberrations” that interfere with image-forming
performance.
Outer Light Ray
(with a larger NA)
Inner Light Ray
(with a smaller NA)
9.2 Classification of Aberrations
Aberrations that interfere with image-forming performance are
classified as shown below in Figure 9-2.
Seidel’s Aberration = “Expansion of a Point Image” +
“Curvature of the Image Plane” + “Deformation”
Figure 9-2
Inner Light Ray
Focal Position
Outer Light Ray
Focal Position
Typically, objectives with a larger NA have better resolution but
worse spherical aberration. Our advanced design and
manufacturing techniques have realized good optical
performance even with a large numerical aperture.
Classification of Aberrations
(2) Coma Aberration
Even though spherical aberration is compensated to be very
small, there are cases where light rays coming out of an offaxis object point are not condensed to a single point on the
image plane but, instead, generate asymmetric blur that looks
like a comet leaving traces. This is called coma aberration.
(1) Spherical Aberration
(2) Coma Aberration
Seidel's
Aberration
Image Plane
(3) Astigmatism
(4) Field Curvature
(5) Distortion
Aberration
Figure 9-4
Chromatic
Aberration
Coma Aberration and Spot Shape
on the Image Plane
(6) Longitudinal (axial)
Chromatic Aberration
(7) Chromatic Aberration
of Magnification
Specimen
Types (1) to (3) correspond to “expansion of a point image”
that goes against requirement (i) for ideal image formation in
Figure 9-1. Type (4) corresponds to “curvature of image plane”
that goes against requirement (ii) in Figure 9-1.
Objective with
Coma Aberration
14-3
Aplanatic
Tube Lens
Image Plane
Optical Terminology
The microscope optical system contains some distortion.
When distortion is present, it can bring erroneous results of
shape measurements. When a microscope is used for
precision measurements, pay close attention to this
aberration, for example, by providing it with an aberration
compensation function.
(3) Astigmatism
Even though a lens is compensated for spherical aberration
and coma aberration, there are cases where an image of an
off-axis object point is not focused to a single point but
separated to a concentric line image and a radial line image.
This is called “astigmatism.” When astigmatism is present, a
point image blurs vertically and horizontally, before and after
the focus position.
Figure 9-5
(6) Chromatic Aberration
Glasses used for optical systems have different refractive
indexes depending on the wavelength. This causes
differences in focal length between wavelengths and
generates displacement of image forming position. This
phenomenon is called “chromatic aberration,” which is
sometimes subdivided into axial displacement on the optical
axis, called “axial chromatic aberration” (or lateral chromatic
aberration) and displacement on the image plane, called
“chromatic aberration of magnitude.”
Many special glass materials are used, e.g., for apochromats,
to eliminate chromatic aberration in a wide range from violet
light (g-rays with wavelength of 435 nm) to red light (c-rays
with wavelength of 656 nm).
Astigmatism and Change in Spot
Shape in Different Focus Positions
(a) (b) (c)
(a)
(b)
(c)
(4) Field Curvature
An image plane of an object on a plane perpendicular to an
optical axis does not always become a plane perpendicular to
the optical axis, but it generally becomes a curved plane. This
symptom is called “field curvature.”
When field curvature is present, the image is more displaced
as it becomes closer to the periphery of the visual field.
Therefore, when the center of an image is brought into focus,
blur occurs in the peripheral areas of the image. To bring the
entire image, including the periphery, into clear focus, it is
necessary to adequately compensate for this type of
aberration.
(5) Distortion
When there is no similar relation between a planar shape on
an object and a shape on the image plane, this is called
“distortion.” When distortion is present, a square image
appears in a shape of a barrel or pin-cushion as shown in
Figure 9-6.
Figure 9-6
Distortion
(a) Barrel Shape
Type
(a) Pin-cushion
Type
14-4
9.3 Wavefront Aberration
For a long time, aberrations have been used in “geometric
optics,” which considers light as “light rays.” Microscope
optical systems are often used to observe very small
specimens at the wavelength level and sometimes adopt
“wave optics,” which regards light as “waves” and handles the
phase information, accounting for the influence of diffraction.
In such a case, “wavefront aberration” is used for evaluation.
As shown below, when requirements for ideal imaging are
satisfied in a microscope optical system, the spherical
wavefront (spherical waves) coming from a single point on an
object (specimen) is converted to plane waves through an
ideal objective. The plane waves are converted to spherical
waves through an ideal tube lens and condensed to a single
point. The wavefront of these waves is called the “ideal
wavefront.”
Figure 9-7
Figure 9-9
Appearance of Condensed Light in
the Image Plane (point spread)
Ideal Microscope Optical System
Specimen Ideal
Objective
Spherical
Wave
9.4 Strehl Ratio
When a point light source is observed with an aberration-free
optical system and an aberrated optical system, the former
concentrates the focal point to a point at the image formation
position. In contrast, the latter fails to produce a focal point,
instead causing a spread in the intensity distribution of the
point image (this is known as “point spread”). The specific
appearance of such a point image (i.e. point spread) is shown
in Figure 9-9.
Ideal
Tube Lens
Plane
Wave
Image Plane
Aberration-Free Optical System
Spherical
Wave
Based on the figure indicated for (1) spherical aberration, the
behavior of the wavefront in an optical system that has an
aberration is described below.
With the proportion of light concentrated in the image plane
(intensity of light concentrated in the Airy disk) by an
aberration-free optical system serving as 100%, the
proportion of light concentrated by an aberrated optical
system is known as the Strehl ratio (SR). When graphed, the
Strehl ratio reveals peaks in intensity as shown in Figure 9-10.
The higher the SR, the closer an optical system is to being
aberration-free.
Figure 9-10
Figure 9-8
Illustration of Wavefront Aberration
Strehl Ratio (SR)
Intensity
Actual
Wavefront
Specimen
Aberrated Optical System
Aberration-Free
Optical System
Ideal
Wavefront
100%
Aberrated
Optical System
Objective with
Spherical Aberration
A difference (a degree of disagreement) between the ideal
wavefront and the actual wavefront shown above is called
“wavefront aberration.”
SR
Image Plane
A Strehl ratio of 80% is typically called the diffraction limit, and
lenses with a lower ratio lack the performance required to
serve as an objective. A ratio of over 95% means that the
lens’ performance in general observations is comparable to
that of an aplanatic lens (which is corrected for spherical
aberrations and coma).
Note: A laser interferometer is used to assess optical performance,
so assessment is done at a single wavelength. Unless otherwise
noted, Strehl ratio measurements are at the e-line (544 nm).
14-5
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