National Optical & Scientific Instruments 420 430PHF-10, 420 AHF-10, 420 1105-10, 420 1107-10, 420T 430PHF-10, 420AHF-10, 1105-10, 1107-10 stereoscopic microscope User manual

National Optical & Scientific Instruments Inc.
6508 Tri-County Parkway
Schertz, Texas 78154
Phone (210) 590-9010 Fax (210) 590-1104
INSTRUCTIONS FOR
STEREOSCOPIC ZOOM MICROSCOPE MODELS
420-430PHF-10 (Binocular Head)
420-420AHF-10 (Binocular Head)
420-1105-10 (Binocular Head)
420-1107-10 (Binocular Head)
420T-430PHF-10 (Trinocular Head)
420T-420AHF-10 (Trinocular Head)
420T-1105-10 (Trinocular Head)
420T-1107-10 (Trinocular Head)
Notice: The first three digits of the model number, for example 420- or 420T-, represent the optical
portion of your microscope. Thus, all models starting with 420 have the same binocular head, and all
models starting with 420T have the same trinocular head. Procedures for use of the head are the same,
regardless of the stand supplied with your specific model.
The digits following the first – (dash) represent the stand portion of your microscope. The different
stands available have features and functions unique to each.
The digits following the second – (dash) represent the power factor of the eyepieces supplied with your
microscope. For example, -10 would indicate that your microscope is supplied with WF10x eyepieces.
You made your selection of stands when you originally purchased your microscope. The above
explanation is provided so that when ordering and installing replacement bulbs you use the correct bulb
for your particular model number.
Copyright © 1/2/01
National Optical & Scientific Instrument Inc.
Illustrated: Model 420
Illustrated: Model 420-430PHF-10
Rubber Eyeshields
Eyepiece Locking Set Screw
Eyepieces
Knurled Diopter Adjustment
(on both eyepieces)
Head
Eyepiece Tube Housing
Power Cord for Top Light
(incidental illumination)
Focusing Assembly
Locking Knob
Head Locking Screw
(head retaining screw located
on opposite side)
Focusing Knob
(both sides)
Tension Adjustment Collar
Knurled Cover Ring
(behind this focusing knob)
Light Shade
Focusing Assembly Locking
Support Collar
Top Light Beam Adjustment Screw
Post
Main Power Switch
Incidental (top)
Light Switch
Stage Clips
Stage Plate
Transmitted (bottom)
Light Switch
(frosted illustrated)
Fuse
Stage Plate Locking Set Screw
Base
Intensity Control for Top Light
Model 420-420AHF-10
Model 420-1105-10
Model 420-1107-10
Stereoscopic microscopes are used for viewing 3-dimensional objects, inspection or assembly of small parts, and for dissection of
biological specimen. They provide an upright, unreversed image which permits easy manipulation of the object being viewed while
looking through the microscope. They are designed for viewing solid objects at low magnification, but they will also permit viewing of
some transparent specimen slides.
For optimum viewing satisfaction, follow these simple procedures. Nomenclature used to describe components and controls can be
identified by referring to the diagram at left.
UNPACKING
1.
Carefully remove microscope stand and head assembly from cartons. Remove rubber eyeshields, blue filter, frosted stage plate,
dustcover, and “L” hex key wrench (used for changing stage plates). The black/white contrast plate (80mm diameter) is already
mounted in the microscope base. (Models with –1105 or –1107 do not include blue filter, frosted stage plate, “L” hex key wrench.
–1107 does have contrast plate.)
2.
Make certain not to touch any of the lens surfaces while handling the microscope. Dust, dirt, fingerprints can damage the delicate
lens surfaces or adversely affect image quality.
3.
Examine packing material before you discard it. Retain the styrofoam container in case you need to transport, store, or
return the microscope for service. If it becomes necessary to ship the microscope for any reason, pack it in the styrofoam
container, and then pack the styrofoam in another corrugated shipping container for optimum protection. Use of the styrofoam
alone will not provide adequate protection in transit, and will void your warranty.
ASSEMBLY
1.
2.
Mounting the stereo zoom head to stand (models with top and bottom illumination):
A.
Loosen head locking screw and head retaining screw.
B.
Insert head into the stand (do not force), positioning head to face either forward or backward, whichever suits your preference
or needs.
C.
Tighten head retaining screw and head locking screw.
D.
Instructions for –1105 and –1107 stands are covered by separate sheet.
Install rubber eyepiece shields over top of eyepieces with the flared portion of the shields positioned at the outside edge
of eyepieces.
OPERATION
1.
ILLUMINATION (only for models with illuminators)
A.
Before operating microscope, adjust intensity control located on side of base to the minimum position. This should be done
prior to each time light is turned on or off. Failure to do so will significantly shorten bulb life.
B.
Make certain that the main voltage of your microscope corresponds to the voltage of your power outlet, either 120v or 220v.
Insert microscope plug into matching voltage outlet.
C.
The microscope is furnished with two stage plates. The frosted glass plate is used when viewing transparent specimen slides
or for viewing some specimen thin enough through which light can pass (insect wings, etc.) The plastic black/white contrast
plate can be used when viewing opaque objects or for dissecting. Choose side of plate providing best contrast with
specimen.
D.
There are three rocker type light controls located on top surface of microscope base.
MAIN
“I”
“T”
= Turns power on and off
= Turns incidental light on (top illumination)
= Turns transmitted light on (substage illumination)
NOTE: USE TRANSMITTED ILLUMINATION ONLY WITH FROSTED GLASS STAGE PLATE AND BLUE FILTER IN
PLACE. HEAT GENERATED IN BASE FROM BOTTOM LIGHT WILL WARP OR DAMAGE THE PLASTIC BLACK/WHITE
PLATE. SUCH DAMAGE WILL NOT BE COVERED BY WARRANTY.
Remove black/white stage plate by loosening locking set screw located on front of base with supplied “L” wrench. Insert
daylight blue filter into machined groove provided in center of base. Install frosted glass stage plate. Tighten locking set
screw.
3
Incidental illumination can be used with either frosted glass plate or black/white plastic stage plate. The top light has an
intensity control located on side of base. When using incidental light, always set intensity control at its lowest level before
turning lamp on or off. This measure will extend bulb life. The top light can also be centered on specimen by using the top
light beam adjustment screw. This allows user to select the best spot illumination required for specimen being viewed.
Transmitted and incidental illumination combined can provide extra illumination for certain objects where additional top
illumination will enhance the object being viewed.
2.
INTERPUPILLARY ADJUSTMENT
This permits each user to adjust spacing between eyepieces in order to accommodate distance between their eyes. While
looking through the microscope eyepieces with both eyes, grasp eyepiece tube housings with both hands and rotate them on
their axis, moving eyepieces apart or together until a full field of view is observed and images blend into one. Interpupillary
distance is now corrected for your own inter-ocular distance and does not require further adjustment later unless another user
changes this adjustment.
3.
FOCUSING
A.
Adjust zoom control knobs (located on both sides of head) so that the lowest magnification number “1” is positioned at the
black index dot on head. Lower magnifications have larger fields of view, making it easier to position and locate area to be
viewed.
B.
Place a flat object or specimen slide (cover glass up), on stage plate.
C.
Position focusing knobs in the center of focusing range.
D.
On post mounted models, the height of viewing head can be adjusted up or down on the post in order to focus on difference
sized objects. Loosen the locking knob located on the locking support collar, allowing the support collar to slide down to
bottom of post. While firmly holding viewing head with one hand, loosen locking knob located on back of focusing assembly
so that head can move freely up or down on post.
While looking through microscope, move viewing head up or down on post until object can be seen in approximate focus.
Tighten focusing assembly locking knob. Position the support collar under the focusing block and tighten locking knob on
support collar. It is not necessary to make this adjustment every time you change objects to be viewed, so long as the
different objects are of similar thickness or height.
E.
On models having the “fixed arm” type stand, the up/down movement of viewing head is limited to the traverse permitted by
the regular focusing knobs. This limits the size object that can be viewed on the stage to about 45mm thickness. Also note
that optional #705-420 0.5x objective lens cannot be used on any 420 series model that is supplied with the “fixed arm” type
stand –420AHF. The 0.5x objective increases the working distance more than the upward traverse permitted by the fixed arm
mount.
F.
Both eyepieces have knurled diopter adjustment rings. Rotate both left and right diopters in a clockwise direction to the
lowest position.
G. Adjust zoom control to the highest magnification by aligning the number “4” on knob to the black index dot on head.
H.
While looking through right eyepiece with one eye, rotate focusing control knob until specimen comes into sharp focus
through right eyepiece.
I.
Adjust zoom control knob to the lowest magnification.
J.
Adjust the right diopter until the image is sharp. Do not change the focusing knob position.
K.
Without changing the position of the focusing knob, adjust the left eyepiece diopter until you obtain a sharp image in left
eyepiece. the image should now be sharp throughout the zoom power range.
4
Specification Chart
Eyepieces
Zoom
Objective
Position
WF5X
Field No.22
(Optional)
(No Reticle Holder)
Interpupillary
Distance
59~83
WF10X
Field No. 20
(Supplied)
Accepts Reticle
23mm O.D.
Interpupillary
Distance
54~78
WF15X
Field No. 13
(optional)
(No Reticle Holder)
Interpupillary
Distance
50.5~74.5
WF20X
Field No. 10
(optional)
(No Reticle Holder)
Interpupillary
Distance
52~76
1X
Stand
-430PHF
-420AHF
-1105
-1107
Standard Objective
1X
(supplied)
Working Distance –
79mm
Total
Field
Size
Magnification
5X
22mm
Auxiliary Objective
0.5X
(Optional)
Working Distance 137mm
Total
Field
Size
Magnification
2.5X
44mm
Auxiliary Objective
0.75X
(Optional)
Working Distance 90mm
Total
Field
Size
Magnification
3.75X
29.3mm
Auxiliary Objective
1.5X
(Optional)
Working Distance 34mm
Total
Field
Size
Magnification
7.5X
14.7mm
2X
10X
11mm
5X
22mm
7.5X
14.6mm
15X
7.3mm
3X
15X
7.3mm
7.5X
14.7mm
11.25X
9.8mm
22.5X
4.9mm
4x
20X
5.5mm
10X
11mm
15X
7.3mm
30X
3.7mm
1X
10X
20mm
5X
40mm
7.5X
26.7mm
15X
13.3mm
2X
20X
10mm
10X
20mm
15X
13.3mm
30X
6.7mm
3X
30X
6.7mm
15X
13.3mm
22.5X
8.9mm
45X
4.4mm
4x
40X
5mm
20X
10mm
30X
6.7mm
60X
3.3mm
1X
15X
13mm
7.5X
26mm
11.25X
17.3mm
22.5X
8.7mm
2X
30X
6.5mm
15X
13mm
22.5X
8.7mm
45X
4.3mm
3X
45X
4.3mm
22.5X
8.7mm
33.75X
5.8mm
67.5X
2.9mm
4x
60X
3.25mm
30X
6.5mm
45X
4.3mm
90X
2.2mm
1X
20X
10mm
10X
20mm
15X
13.3mm
30X
6.7mm
2X
40X
5mm
20X
10mm
30X
6.7mm
60X
3.3mm
3X
60X
3.3mm
30X
6.7mm
45X
4.4mm
90X
2.2mm
4x
80X
2.5mm
40X
5mm
60X
3.3mm
120X
1.7mm
Specimen Height
Minimum
Maximum
0.0mm
93mm
0.0mm
45mm
0.0mm
437mm
0.0mm
97mm
Specimen Height
Minimum
Maximum
0.0mm
19mm
Inoperative
Inoperative
0.0mm
363mm
0.0mm
23mm
5
Specimen Height
Minimum
Maximum
0.0mm
66mm
0.0mm
18mm
0.0mm
410mm
0.0mm
70mm
Specimen Height
Minimum
Maximum
0.0mm
120mm
27mm
72mm
00mm
464mm
0.0mm
124mm
4.
ADAPTING SLR OR C-MOUNT CAMERA (to trinocular model only)
A.
Trinocular model #420T is equipped with a port (a.) on top of binocular head. By
using optional accessory adaptors, either SLR or C-mount cameras can be mounted
onto the microscope.
These models are also equipped with a sliding rod (b.) located on the back side of
the head. When this rod is pushed completely into the head, the microscope image
is directed 100% into both eyepieces of the microscope. When this rod is pulled as
far as possible away from the head, the microscope image is directed into the
trinocular port. You will still be able to observe image through left eyepiece, but no
image will be visible through right eyepiece.
B.
T-mount
SLR
adaptor
(a.)
To mount SLR camera, the accessory #930-420 SLR adaptor (included) is required,
along with a T-mount available from any camera store. This accessory has a 2.0x
photo lens incorporated within the adaptor.
(c.)
(c.)
(b.)
Remove front lens of SLR camera. Attach appropriate T-mount in place of front
camera lens. Screw threaded end of T-mount onto threaded end of SLR adaptor.
Locate two knurled screws (c.) located on side of trinocular port on microscope. Turn both screws counter-clockwise to
permit removal of black plastic disk covering trinocular port.
Insert SLR adaptor tube, with camera already mounted to adaptor, into trinocular port. If adaptor does not insert easily,
further loosen knurled screws at side of port until adaptor tube drops into port and is firmly seated. Retighten knurled screws
to secure adaptor and camera in place. Pull sliding rod (b.) until fully extended, to direct microscope image to trinocular port.
Proceed with operation of camera according to manufacturers directions.
C.
To mount C-mount ready camera, the accessory #930-421 video adaptor (included) is required. This adaptor has a 0.4x lens
which assures image parfocality when viewed through a video monitor.
Observe that C-mount adaptor has two black knurled rings. If your camera has a 1/2 inch chip, leave both knurled rings in
place, thereby creating a “CS” type mount. If your camera has a 1/3 inch chip, remove only the top black knurled ring from
C-mount by turning counter-clockwise. The remaining black knurled ring is a “C” type adaptor. You may also purchase the
optional 0.5x lens, accessory #930-422, designed specifically for 1/2 inch chips. It is designed with an adjustable focusing
lens for parfocality.
Thread the front of the camera onto threads of C-mount adapter.
Locate two knurled screws (c.) located on side of trinocular port on microscope. Turn both
screws counter-clockwise to permit removal of black plastic disk covering trinocular port.
(c.)
(b.)
Insert C-mount adapter into trinocular port. If adaptor does not insert easily, further loosen
knurled screws (c.) at side of port until adaptor tube drops into port and is firmly seated.
Retighten knurled screws to secure adaptor and camera in place. Pull sliding rod (b.) until
fully extended, to direct microscope image to trinocular port.
6
MAINTENANCE
WARNING: For your own safety, turn switch off and remove plug from power source before maintaining your microscope. If
the power cord is worn, cut or damaged in any way, have it replaced immediately to avoid shock or fire hazard.
1. OPTICAL MAINTENANCE
2.
A.
Do not attempt to disassemble any lens components. Consult a microscope service technician when any repairs not covered
by instructions are needed.
B.
Prior to cleaning any lens surface, brush dust or lint off lens surface using a camel hair brush. You can also use an ear
syringe or canned compressed air, such as that sold by most computer stores.
C.
To clean eyepiece lenses, do not remove from eyepiece tube. Clean only the outer lens surface. Breath on lens to dampen
surface, then wipe with lens paper or tissue or use a cotton swab moistened with distilled water. Wipe lenses with a circular
motion, applying as little pressure as possible. Avoid wiping dry lens surface as lenses are scratched easily. If excessive dirt
or grease gets on lens surfaces, a small amount of Windex can be used on a cotton swab or lens tissue. To clean objective
lenses, do not remove objectives from microscope. Clean front lens element only, following same procedure.
MECHANICAL MAINTENANCE
The only mechanical adjustment the microscope might require is the tension of the focusing mechanism. This has been
adjusted at the factory, but over the course of time it may loosen and cause the head of the microscope to slip downward on
the focusing block.
The tension adjustment collar is located between arm and focus knob on left side of microscope. With a jewelers type
screwdriver, loosen slotted set screw located on knurled surface of the tension adjustment collar. Turn collar clockwise to
tighten tension, counter-clockwise to loosen tension. After adjusting, tighten the set screw to lock collar in place.
NOTE: It is recommended that you leave the tension as loose as possible for ease of focusing, yet not so loose that it
permits the head of microscope to drift downward from its own weight and cause the microscope to “drift” out of focus.
3.
ELECTRICAL MAINTENANCE (only for models with illumination)
The extent of electrical maintenance, by other than a qualified technician, should be bulb replacement. BE CERTAIN TO TURN
SWITCHES OFF AND REMOVE PLUG FROM POWER SOURCE OUTLET BEFORE CHANGING BULBS.
A.
To replace top bulb (National bulb #800-422) .... remove light shade by rotating in a counter-clockwise direction. Remove
light housing by rotating in a counter-clockwise direction. Remove light bulb by firmly grasping and pulling straight out from bipin socket. Note that this socket holds bulb securely, so you might have to pull rather firmly. Using a cloth, hold new bulb
and gently push new bulb into bi-pin socket. Replace light housing and light shade.
B.
To replace bottom bulb in stand –430PHF & -420AHF (National bulb #800-138) .... remove cover plate located on bottom of
base by removing four rubber feet that secure cover to base. Holding fluorescent lamp with a cloth, gently pull lamp straight
out from socket. Push new lamp into place in same manner and replace cover plate.
C.
Replacement of fuse.
The fuse is located at right rear side of microscope base. To remove fuse from holder, insert a 6mm screwdriver blade into
slot located in rear of fuse holder cap. Slightly depress and rotate screwdriver ¼ turn in direction of arrow, release pressure
on screwdriver to release the fuse. Pull cap and fuse out of fuse holder. Insert proper fuse into fuse cap. Insert fuse cap into
fuse holder. Using screwdriver, rotate fuse cap assembly in opposite direction of arrow until guide slot engages, depress fuse
cap and rotate ¼ turn to lock into fuse holder.
7
TROUBLESHOOTING
PROBLEM
Light fails to operate.
Image does not remain in focus
Poor resolution
(image not sharp)
REASON FOR PROBLEM
Outlet inoperative.
SOLUTION
Have qualified service technician
repair outlet.
AC power cord not connected.
Plug into outlet.
Lamp burned out.
Replace lamp.
Fuse blown.
Head of microscope drops from its
own weight.
Objective lenses dirty.
Replace fuse.
Adjust tension control.
Clean objective lenses.
Eyepiece lens dirty.
Clean eyepiece lenses.
Spots in field of view.
Eyepiece lens dirty.
Clean eyepiece lenses. ***
***Spots in field of view can also result from dirt on inside of eyepiece. It is recommended that you have service
technician clean inside of lens.
OPTIONAL ACCESSORIES AND PARTS:
#600-400
#605-400
#615-400
#620-400
#705-420
#775-420
#715-420
#800-422
#800-138
#800-907
#801-050
#801-100
#908-LED
#930-420
#930-421
#930-422
#931-409
#931-420
#965-400-05
#965-400-10
#975-001
Eyepiece shields, rubber, pair
WF5X Eyepieces
WF15X Eyepieces
WF20X Eyepieces
Auxiliary 0.5x objective lens (Do not use with stands –420AHF)
Auxiliary 0.75x objective lens (Allows only limited maximum specimen height with stands –420AHF)
Auxiliary 1.5x objective lens (Minimum specimen height – 27mm on stands –420AHF)
Replacement bulb, top light, 12v 15 watt halogen bi-pin
Replacement bulb, bottom light, 115v 5 watt fluorescent
Replacement bulb for 907 fluorescent ring light
Replacement fuse for 220v version, 0.5 amp
Replacement fuse for 117v version, 1.0 amp
LED ring light illuminator, requires #931-420 ring light adaptor
SLR adaptor with 2.0x photo lens
Video “C” mount with .04x lens
Video “C” mount adapter with 0.5x lens (for ½” chip sensor)
Blue filter, frosted 35.6mm O.D.
Ring light adapter, O.D. 54.5mm (permits mounting auxiliary ring light on objective pod)
Eyepiece reticle, 5mm/100 divisions, O.D. 23mm (for use only with WF10x eyepieces)
Eyepiece reticle, 10mm/100 divisions, O.D. 23mm (for use only with WF10x eyepieces)
Carrying case, anodized aluminum, fabric lining, accessory pockets, Velcro straps, keyed lock. Note: Fits all 420
series except 420-1105 & 420-1107. No case available for these models.
LIMITED LIFETIME WARRANTY
Please see our website, www.nationaloptical.com, for complete warranty details and exclusions.
www.microscopeworld.com
[email protected]
800-942-0528
8
(Revised 2/11/14)