DiaSorin Kit 1,25-Dihydroxyvitamin D RIA Instruction manual

Below you will find brief information for 1,25-Dihydroxyvitamin D RIA Kit. This kit is intended for the quantitative determination of 1,25-dihydroxyvitamin D (1,25-(OH)2-D) in human serum or EDTA plasma to be used to assess 1,25-(OH)2-D deficiency associated with renal disease.

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1,25-Dihydroxyvitamin D RIA Kit Instruction Manual | Manualzz

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Key features

  • Quantitative determination of 1,25-dihydroxyvitamin D
  • Assay performed in human serum or EDTA plasma
  • Assessment of 1,25-(OH)2-D deficiency associated with renal disease
  • Two-step procedure involving extraction and RIA
  • Uses a polyclonal antibody specific for 1,25-(OH)2 D2 and 1,25-(OH)2D3

Frequently asked questions

The 1,25-Dihydroxyvitamin D 125I RIA is intended for the quantitative determination of 1,25-dihydroxyvitamin D (1,25-(OH)2-D) in human serum or EDTA plasma to be used to assess 1,25-(OH)2-D deficiency associated with renal disease.

The assay involves a preliminary extraction and subsequent purification of vitamin D metabolites from serum or EDTA plasma using C18OH cartridges. Following extraction, the treated sample is then assayed using a competitive RIA procedure.

Counting times should be sufficient to prevent the introduction of error due to counter inefficiency. Assay results should be used in conjunction with other clinical and laboratory data to assist the clinician in making individualized patient management decisions. The performance characteristics of this assay have not been established in a pediatric population.
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