Hanna Instruments | HI83306-01 | Owner Manual | Hanna Instruments HI83306-01 Owner Manual

Hanna Instruments HI83306-01 Owner Manual
Environmental Analysis Photometer
INSTRUCTION MANUAL
HI83306
Dear
Customer,
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the instrument.
This manual will provide you with the necessary information for correct use of the
instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail us at
tech@hannainst.com or view our worldwide contact list at www.hannainst.com.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of
the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
TABLE OF CONTENTS
1. PRELIMINARY EXAMINATION............................................................................................. 5
2. SAFETY MEASURES........................................................................................................... 5
3. SPECIFICATIONS............................................................................................................... 6
4. DESCRIPTION................................................................................................................... 7
4.1. GENERAL DESCRIPTION............................................................................................. 7
4.2. PRECISION AND ACCURACY........................................................................................ 7
4.3. FUNCTIONAL DESCRIPTION........................................................................................ 8
4.4. PRINCIPLE OF OPERATION......................................................................................... 9
4.5. OPTICAL SYSTEM...................................................................................................... 10
5. GENERAL OPERATIONS...................................................................................................... 11
5.1. POWER CONNECTION AND BATTERY MANAGEMENT...................................................... 11
5.2. GENERAL SETUP........................................................................................................ 11
5.3. USING HANNA DIGITAL ELECTRODES........................................................................... 14
5.4. MODE SELECTION..................................................................................................... 14
5.5. LOGGING DATA......................................................................................................... 15
5.6. ADDING SAMPLE / USER NAMES TO LOG DATA............................................................ 15
5.7. DATA MANAGEMENT.................................................................................................. 16
5.8. CONTEXTUAL HELP.................................................................................................... 17
6. PHOTOMETER MODE......................................................................................................... 17
6.1. METHOD SELECTION................................................................................................. 17
6.2. COLLECTING AND MEASURING SAMPLES AND REAGENTS.............................................. 18
6.2.1. PROPER USE OF SYRINGE................................................................................... 18
6.2.2. PROPER USE OF DROPPER................................................................................... 18
6.2.3. PROPER USE OF POWDER PACKET......................................................................... 18
6.3. CUVETTE PREPARATION............................................................................................. 19
6.4. TIMERS AND MEASUREMENT FUNCTIONS................................................................... 20
6.5. CHEMICAL FORMULA / UNIT CONVERSIONS................................................................ 21
6.6. METER VALIDATION / CAL CHECK................................................................................ 21
6.7. ABSORBANCE MEASUREMENTS.................................................................................. 22
7. PROBE MODE................................................................................................................... 23
7.1. pH CALIBRATION...................................................................................................... 23
7.2. pH CALIBRATION MESSAGES...................................................................................... 25
7.3. pH MEASUREMENT................................................................................................... 25
7.4. pH MEASUREMENT MESSAGES / WARNINGS............................................................... 27
7.5. pH GLP..................................................................................................................... 28
7.6. pH ELECTRODE CONDITIONING AND MAINTENANCE..................................................... 29
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TABLE OF CONTENTS
4
8. METHOD PROCEDURES................................................................................................. 31
8.1. AMMONIA LOW RANGE.............................................................................................. 31
8.2. AMMONIA MEDIUM RANGE........................................................................................ 34
8.3. AMMONIA HIGH RANGE............................................................................................. 37
8.4. CHLORINE, FREE....................................................................................................... 40
8.5. CHLORINE, TOTAL..................................................................................................... 43
8.6. CHROMIUM (VI) LOW RANGE..................................................................................... 46
8.7. CHROMIUM (VI) HIGH RANGE.................................................................................... 48
8.8. COLOR OF WATER..................................................................................................... 50
8.9. COPPER LOW RANGE................................................................................................. 52
8.10. COPPER HIGH RANGE.............................................................................................. 54
8.11. CYANURIC ACID...................................................................................................... 56
8.12. MOLYBDENUM....................................................................................................... 58
8.13. NICKEL LOW RANGE................................................................................................ 61
8.14. NICKEL HIGH RANGE............................................................................................... 64
8.15. NITRATE................................................................................................................ 66
8.16. NITRITE HIGH RANGE.............................................................................................. 69
8.17. OXYGEN, DISSOLVED............................................................................................... 71
8.18. pH........................................................................................................................ 74
8.19. PHOSPHATE LOW RANGE......................................................................................... 76
8.20. PHOSPHATE HIGH RANGE........................................................................................ 78
8.21. SILICA LOW RANGE................................................................................................. 81
8.22. SILVER.................................................................................................................. 84
8.23. ZINC...................................................................................................................... 88
9. WARNINGS & ERRORS...................................................................................................... 91
10. STANDARD METHODS..................................................................................................... 94
11. ACCESSORIES................................................................................................................ 95
11.1. REAGENT SETS....................................................................................................... 95
11.2. pH ELECTRODES..................................................................................................... 97
11.3. pH SOLUTIONS....................................................................................................... 98
11.4. OTHER ACCESSORIES.............................................................................................. 99
12. ABBREVIATIONS............................................................................................................ 101
CERTIFICATION.................................................................................................................... 102
RECOMMENDATIONS FOR USERS.......................................................................................... 103
WARRANTY......................................................................................................................... 103
PRELIMINARY EXAMINATION
1. PRELIMINARY EXAMINATION
Remove the instrument and accessories from the packaging and examine it carefully to make sure
that no damage has occurred during shipping. Notify your nearest Hanna Customer Service Center if
damage is observed.
Each HI83306 is supplied with:
• Sample Cuvette and Cap (4 pcs.)
• Cloth for Wiping Cuvettes
• Scissors
• USB Cable
• 5 Vdc Power Adapter
• Instruction Manual
• DO bottle (glass stopper bottle)
• Quality Certificate
Note: Save all packing material until you are sure that the instrument works correctly.
Any damaged or defective item must be returned in its original packing material with the
supplied accessories.
SAFETY MEASURES
2. SAFETY MEASURES
•The chemicals contained in the reagent kits may be hazardous if improperly
handled.
•Read the Safety Data Sheets (SDS) before performing tests.
• Safety equipment: Wear suitable eye protection and clothing when required, and follow
instructions carefully.
• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty of water. If
reagent contacts skin, rinse the affected area thoroughly with water. Avoid breathing released
vapors.
• Waste disposal: for proper disposal of reagent kits and reacted samples, contact a licensed
waste disposal provider.
5
SPECIFICATIONS
3. SPECIFICATIONS
Measurement Channels
Absorbance
Range
Resolution
Accuracy
Light Source
Bandpass Filter Bandwidth
4 x optical channels
1 x digital electrode channel (pH measurement)
0.000 to 4.000 Abs
0.001 Abs
±0.003 Abs (at 1.000 Abs)
light emitting diode
8 nm
Bandpass Filter Wavelength Accuracy ±1.0 nm
pH
Light Detector
Cuvette Types
Number of Methods
Range
Resolution
Accuracy
Temperature Compensation
Calibration
Temperature
Additional
Specifications
Electrode
Range
Resolution
Accuracy
Logging
Display
USB-A (Host) Functions
USB-B (Device) Functions
Battery Life
Power Supply
Environment
Dimensions
Weight
silicon photocell
round, 24.6 mm diameter
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-2.00 to 16.00 pH (± 1000.0 mV)*
0.01 pH (0.1 mV)
±0.01 pH (±0.2 mV) (@ 25 °C / 77 °F)
ATC (-5.0 to 100.0 °C; 23.0 to 212.0 °F)*
2 points, eligible from 5 available buffers (4.01,
6.86, 7.01, 9.18, 10.01 pH)
Intelligent pH / temperature electrode
-20.0 to 120.0°C (-4.0 to 248.0 °F)
0.1 °C (0.1 °F)
±0.5 °C (±0.9 °F) (@ 25 °C / 77 °F)
1000 readings (mixed photometer and electrode)
128 x 64 pixel B/W LCD with backlight
mass-storage host
power input, mass-storage device
> 500 photometer measurements, or 50 hours of
continuous pH measurement
5 Vdc USB 2.0 power adapter/type micro-B connector
3.7 Vdc Li-polymer rechargeable battery,
non-serviceable
0 to 50 °C (32 to 122 °F);
0 to 95% RH, non-serviceable
206 x 177 x 97 mm (8.1 x 7.0 x 3.8")
1.0 kg (2.2 lbs.)
*Limits will be reduced to actual probe/sensor limits.
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DESCRIPTION
4. DESCRIPTION
4.1.GENERAL DESCRIPTION
HI83306 multiparameter photometer is compact and versatile meter with two measurement
modes: Absorbance and pH/ mV. Absorbance mode include CAL Check feature and 27 different
methods that cover a wide variety of applications, making it ideal for both benchtop and portable
operation.
• Digital electrode input for pH measurements
• Certified CAL Check cuvettes to confirm meter functionality
• Dual purpose micro-USB flash drive
• Li-polymer rechargeable battery
• Auto-off
• Absorbance mode
• User and sample name entry
• GLP features
4.2.PRECISION AND ACCURACY
Precision is how closely repeated measurements
are to one another. Precision is usually expressed
as standard deviation (SD).
Accuracy is defined as the closeness of a test
result to the true value.
Although good precision suggests good accuracy,
precise results can be inaccurate. The figure
explains these definitions.
For each method, the accuracy is expressed in the
related measurement section.
7
DESCRIPTION
4.3. FUNCTIONAL DESCRIPTION
1)
2)
3)
4)
5)
6)
7)
8)
9)
10)
8
Splash-proof keypad
Liquid Crystal Display (LCD)
Indexing mark
Protective port covers
Light-blocking cover panel
Cuvette holder
ON/OFF power button
3.5 mm TRRS (jack) input for digital electrodes
Standard USB host connector for data transfer to a USB flash drive
Micro-USB device connector for power or PC interface
Press the functional keys to perform the function displayed above them on the LCD.
Press to access the list of photometer methods.
Press to move up in a menu or a help screen, to increment a set value, or to access
second level functions.
DESCRIPTION
Keypad Description
The keypad contains 12 direct keys and 3 functional keys with the following functions:
Press to toggle between photometer and pH (electrode) mode.
Press to move left in a menu or to decrement a set value.
Press to move down in a menu or a help screen, to decrement a set value, or to access
second level functions.
Press to move right in a menu or to increment a set value.
Press to access the setup screen.
Press to log the current reading.
Press to review saved logs.
Press to exit the current screen.
Press to display the help screen.
ON/OFF power button.
4.4. PRINCIPLE OF OPERATION
Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms,
molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the physical-chemical characteristics of the substance according to the
Lambert-Beer Law:
-log I/Io = el c d
or
A = el c d
9
DESCRIPTION
= intensity of incident light beam
Io
I
= intensity of light beam after absorption
el
= molar extinction coefficient at wavelength λ
c
= molar concentration of the substance
d
= optical path through the substance
Therefore, the concentration "c" can be calculated from the absorbance of the substance as the
other factors are constant.
Photometric chemical analysis is based on specific chemical reactions between a sample and
reagent to produce a light-absorbing compound.
4.5. OPTICAL SYSTEM
Instrument Block Diagram
The internal reference system (reference detector) of the HI83306 photometer compensates for any
drifts due to power fluctuations or ambient temperature changes, providing a stable source of light
for your blank (zero) measurement and sample measurement.
LED light sources offer superior performance compared to tungsten lamps. LEDs have a much higher
luminous efficiency, providing more light while using less power. They also produce little heat,
which could otherwise affect electronic stability. LEDs are available in a wide array of wavelengths,
whereas tungsten lamps have poor blue/violet light output.
Improved optical filters ensure greater wavelength accuracy and allow a brighter, stronger signal to
be received. The end result is higher measurement stability and less wavelength error.
A focusing lens collects all of the light that exits the cuvette, eliminating errors from cuvette
imperfections and scratches, eliminating the need to index the cuvette.
10
DESCRIPTION
5. GENERAL OPERATIONS
5.1. POWER CONNECTION AND BATTERY MANAGEMENT
The meter can be powered from an AC/DC adapter (included) or from the built-in rechargeable battery.
The meter will perform an auto-diagnostic test when it is first powered on. During this test, the
HANNA® logo will appear on the LCD. After 5 seconds, if the test was successful, the last method
used will appear on the display. The battery icon on the LCD will indicate the battery status:
- battery is charging from external adapter- battery fully charged (meter connected to AC/DC adapter)
- battery capacity (no external adapter)- battery near 0% (no external adapter)
- battery exhausted (no external adapter)
To conserve battery, the meter will turn off automatically after 15 minutes of inactivity (30 minutes
before a READ measurement). If a photometer measurement is on the screen, an auto-log is
created before shutdown.
5.2. GENERAL SETUP
Press SETUP key to enter in Setup menu, highlight desired option using
and press Select.
CAL Check (Photometer Only)
Press Select to enter the CAL Check screen. The date, time and
values for the last CAL Check are displayed on the screen.
To start a new CAL Check press Check key and follow the prompts
on the screen.
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GENERAL OPERATIONS
Temperature Unit (pH Only)
Option: °C or °F
Press the functional key to select the desired temperature unit.
Backlight
Values: 0 to 8
Press the Modify key to access the backlight intensity.
Use the functional keys or the   keys to increase or decrease
the value.
Press the Accept key to confirm or ESC to return to the Setup
menu without saving the new value.
Contrast
Values: 0 to 20
Press the Modify key to change the display’s contrast.
Use the functional keys or the   keys to increase or decrease
the value.
Press the Accept key to confirm the value or ESC to return to the
Setup menu without saving the new value.
Date / Time
Press the Modify key to change the date/time.
Press the functional keys or the   keys to highlight the value
to be modified (year, month, day, hour, minute or second).
Use the   keys to change the value.
Press the Accept key to confirm or ESC to return to the Setup
without saving the new date or time.
Time Format
Option: AM/PM or 24-hour
Press the functional key to select the desired time format.
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Decimal Separator
Option: Comma ( , ) or Period ( . )
Press the functional key to select the desired decimal separator.
The decimal separator is used on the measurement screen and
CSV files.
GENERAL OPERATIONS
Date Format
Press the Modify key to change the Date Format.
Use the   keys to select the desired format.
Press the Select key to confirm or ESC to return to the Setup menu
without saving the new format.
Language
Press the Modify key to change the Language. Use the  
keys to select the desired language.
Press Select to choose one of the 7 languages installed.
Beeper
Option: Enable or Disable
When enabled, a short beep is heard every time a key is pressed.
A long beep alert sounds when the pressed key is not active or an
error is detected. Press the functional key to enable/disable the
beeper.
Instrument ID
Option: 0 to 999999
This option is used to set the instrument’s ID (identification
number). Press the Modify key to access the instrument ID screen.
Use the functional keys or the  keys to highlight the digit
to be modified. Press the   keys in order to set the desired
value. Press the Accept key to confirm the value or ESC to return
to the Setup menu without saving the new value.
13
GENERAL OPERATIONS
Meter Information
Press the Select key to view the model, serial number, firmware
version and selected language. Press ESC to return to the Setup
menu.
Probe Information (pH mode only)
Press the Select key to view model number, serial number and
firmware version for the connected probe.
Press ESC to return to the Setup menu.
5.3. USING HANNA DIGITAL ELECTRODES
The HI83306 can be used to perform direct pH measurements by connecting a HANNA® digital
pH electrode with a 3.5 mm TRRS connector. To begin taking probe measurements, connect the
electrode to the 3.5 mm port marked with “EXT PROBE” located at the rear of the meter. If the
meter is in “Photometer Mode”, set the meter to “Probe Mode” by pressing the MODE key.
5.4. MODE SELECTION
The HI83306 has two operational modes: Photometer Mode and Probe Mode.
Photometer Mode enables on-demand measurement of a cuvette using the integrated optical
system. Photometric-related functions, such as Method selection, Zero, Read, and Timers are
available in this mode.
Probe Mode enables continuous measurement using a Hanna Digital Electrode connected to the
3.5 mm port. Probe-related functions, such as calibration and GLP, are available in this mode.
To switch between Photometer Mode and Probe Mode, use the
button.
Note: The active mode cannot be switched while in menus, such as Setup, Recall, Method, etc.
14
GENERAL OPERATIONS
5.5. LOGGING DATA
The instrument features a data log function to help you keep track of all your analysis. The data log
can hold 1000 individual measurements. Storing, viewing and deleting the data is possible using
the LOG and RECALL keys.
Storing data: You can store only a valid measurement. Press LOG and the last valid
measurement will be stored with date and time stamp.
5.6. ADDING SAMPLE / USER NAMES TO LOG DATA
A sample ID and user ID can be added to the saved log. Use the  keys to highlight the
Sample ID or User ID then press Modify.
Text Entry
Sample ID and User ID care entered using the alphanumeric multi-tapping keypad.
Enter one character at a time by pressing the key with the assigned character repeatedly until the
desired character is highlighted. For reference, a list of the characters available for the current key
will be shown under the text box.
The character will be entered after a two-second delay or after another key is pressed.
Once all characters have been entered, press Accept to use the displayed text.
15
GENERAL OPERATIONS
The following functions are available during Text Entry:
• Accept: Press to accept the current displayed text.
• Arrow: Press to delete the last character.
• Clear: Press to delete all characters.
Press to discard all changes and return to the previous screen.
5.7. DATA MANAGEMENT
Viewing and deleting: You can view, export and delete the data by pressing the RECALL key. Use
the  keys to scroll through the saved logs. Press Info to view additional information about
the selected log.
Data Export:
Log data can be exported to a USB flash drive or to a PC. To access Data Export functions, press
Recall then Export.
Use the   keys to select the desired export location.
For export to USB Flash Drive, insert the USB Flash Drive into the dedicated port at the back of the
meter labeled HOST USB, then follow the on-screen prompts.
For export to PC, connect the meter to a PC using the supplied micro-USB cable. Insert the cable
into the port at the back of the meter labeled PC PWR. Follow the on-screen prompts. When the
meter says PC connected, use a file manager (such as Windows Explorer or Mac Finder) to move the
file from the meter to the PC. The meter will appear as a removable disk.
16
5.8. CONTEXTUAL HELP
HI83306 offers an interactive contextual help mode that assists the user at any time.
To access the help screen press HELP.
The instrument will display additional information related to the
current screen. To read all the available information, scroll the text
using the   keys.
To exit help mode press ESC key and the meter will return to the
previous screen.
PHOTOMETER MODE
6. PHOTOMETER MODE
6.1. METHOD SELECTION
In order to select the desired method press the METHOD key and a screen with the available
methods will appear.
Press the  keys to highlight the desired method. Press Select.
GENERAL OPERATIONS
Log data is exported as a single file containing all logged photometer and probe data. The file
name is: “HI83306.csv”. The CSV file (Comma-Separated Values) may be opened with a text
editor or spreadsheet application.
After the desired method is selected, follow the procedure described in the related section.
Before performing a method read all the instructions carefully.
17
PHOTOMETER MODE
6.2. COLLECTING AND MEASURING SAMPLES AND REAGENTS
6.2.1. PROPER USE OF SYRINGE
(a) Push the plunger completely into the syringe and insert the tip
into the solution.
(b) Pull the plunger up until the lower edge of the seal is exactly on
the mark for the desired volume.
(c) Take out the syringe and clean the outside of the syringe tip, be
sure that no drops are hanging on the tip of the syringe. Then,
keeping the syringe in vertical position above the cuvette, push
the plunger down into the syringe, the desired volume has been
delivered into the cuvette.
6.2.2. PROPER USE OF DROPPER
(a) For reproducible results, tap the dropper on the table several times and wipe the outside of the
tip with a cloth.
(b) Always keep the dropper bottle in a vertical position while dosing the reagent.
6.2.3. PROPER USE OF POWDER PACKET
(a) Use scissors to open the powder packet
(b) Push the edges of the packet to form a spout
(c) Pour out the content of the packet.
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PHOTOMETER MODE
6.3. CUVETTE PREPARATION
Proper mixing is very important for reproducibility of the measurements. The proper mixing
technique for each method is listed in the method procedure.
(a) Invert the cuvette a couple of times or for a specified time: hold the cuvette in the vertical
position. Turn the cuvette upside-down and wait for all of the solution to flow to the cap end,
then return the cuvette to the upright vertical position and wait for all of the solution to flow to
the cuvette bottom. This is one inversion. The correct speed for this mixing technique is
10-15 complete inversions in 30 seconds.
This mixing technique is indicated with “invert to mix” and the following icon:
(b) Shaking the cuvette, moving the cuvette up and down. The movement may be gentle or
vigorous. This mixing method is indicated with “shake gently” or “shake vigorously”, and one
of the following icons:
shake gently
shake vigorously
In order to avoid reagent leaking and to obtain more accurate measurements,
and then the
close the cuvette first with the supplied HDPE plastic stopper
black cap.
Whenever the cuvette is placed into the measurement holder, it must be dry
outside and free of fingerprints, oil or dirt. Wipe it thoroughly with HI731318 or
a lint-free cloth prior to insertion.
Shaking the cuvette can generate bubbles in the sample, causing higher
readings. To obtain accurate measurements, remove such bubbles by swirling or
by gently tapping the cuvette.
19
PHOTOMETER MODE
Do not let the reacted sample stand too long after reagent is added. For best accuracy, respect the
timings described in each specific method.
It is possible to take multiple readings in a row, but it is recommended
to take a new zero reading for each sample and to use the same cuvette
for zeroing and measurement when possible.
Discard the sample immediately after the reading is taken, or the glass
might become permanently stained.
All the reaction times reported in this manual are at 25 °C (77 °F). In
general, the reaction time should be increased for temperatures lower
than 20 °C (68 °F), and decreased for temperatures higher than 25 °C
(77 °F).
Interference
In the method measurement section the most common interferences that may be present in a
typical water sample have been reported. It is possible that a particular application could introduce
other compounds that will also interfere.
6.4. TIMERS AND MEASUREMENT FUNCTIONS
Each method requires a different preparation procedure, reaction times, sample preparations, etc. If
a timer or timers are necessary for proper sample preparation, the Timer key will be available.
To use a reaction timer, press the Timer key.
The default timer will start immediately. To stop and reset the timer, press Stop.
If the selected method requires more than one timer, the meter will automatically select each timer
in the appropriate order. To bypass the default order, you may press the desired key to activate a
different timer (only while the current timer is stopped). Press Continue to start the active timer.
For some methods, the timer is only necessary after a Zero measurement has been performed. In
this case, the timer key will only be available after the Zero measurement has been performed.
If the method requires a Zero or Read measurement after a timer has expired, the meter will
automatically perform the appropriate action. Follow the instructions in the Method Procedure.
To perform a Zero or Read measurement, insert the appropriate prepared cuvette, then press the
Zero or Read key. A Zero measurement must be conducted before Read measurements.
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PHOTOMETER MODE
6.5. CHEMICAL FORMULA / UNIT CONVERSION
Chemical formula/unit conversion factors are pre-programmed into the instrument and are method
specific. In order to view the displayed result in the desired chemical formula press  keys to
access the second level function and then press the Chem Frm key to toggle between the available
chemical formulas for the selected method.
6.6. METER VALIDATION / CAL CHECK
WARNING: Do not validate the meter with standard solutions other than the HANNA® CAL Check
Standards. For accurate validation results, please perform tests at room temperature (18 to 25 °C;
64.5 to 77.0 °F).
Validation of the HI83306 involves absorbance measurements of certified HANNA® CAL Check
Standards (see “Accessories”). The “CAL Check” screen guides the user through the measurement of
each CAL Check Standard and applies the factory calibration corrections to each measurement. The
HI83306 stores the results of the most recent CAL Check measurements which may be viewed on
the “CAL Check” screen. Compare these results with the values printed on the Certificate provided
with each HANNA® CAL Check Standards kit.
To perform a validation:
1. Press Setup button.
2. Highlight CAL Check, then press Select.
3. Follow the prompts on the screen. The meter will prompt to
measure each cuvette provided in the HANNA® CAL Check
Standards kit. To abort the process at any time, press ESC
button.
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PHOTOMETER MODE
4. Press ESC to return in Setup menu.
6.7. ABSORBANCE MEASUREMENTS
Raw absorbance measurements may be performed on the HI83306 for personal or diagnostic
purposes. For example, you may monitor the stability of a reagent blank by occasionally measuring
its absorbance versus deionized water.
To measure the raw absorbance of a prepared sample:
1. Enable “Photometer Mode” if necessary by pressing the MODE key.
2. Press the METHOD key.
3. Highlight the appropriate Absorbance method (according to the wavelength to be used), then
press Select. Absorbance methods are located at the bottom of the method list.
4. Prepare the sample cuvette according to the method.
5. Insert a cuvette filled with deionized water, then press Zero.
6. Insert the prepared sample cuvette, then press Read.
WARNING: Never use Absorbance methods for validation using HANNA® CAL Check cuvettes. The
factory calibration corrections for CAL Check cuvettes are applied while in CAL Check mode only!
22
Press Calibrate to access electrode calibration functions.
PHOTOMETER MODE
7. PROBE MODE
7.1. pH CALIBRATION
Press MODE to enter in pH/ mV measurement mode.
Calibration Mode
While in pH Calibration Mode, the display will show the current pH reading, the current
temperature reading, the current selected buffer, and the buffer number (“Buffer: 1” for the
1st buffer, “Buffer: 2” for the 2nd buffer).
The following functions are available in pH Calibration Mode:
• Clear: Press to clear the current calibration from the probe.
• Confirm: Press to accept the current calibration point. Only
available if the measurement is stable and within the limits
for the selected buffer.
Press to cycle through the list of available buffers: 4.01, 6.86, 7.01, 9.18, 10.01 pH.
Press to exit calibration and return to pH Measurement Mode.
23
PROBE MODE
Preparation
Pour small quantities of the buffer solutions into clean beakers. If possible, use plastic beakers to
minimize any EMC interferences. For accurate calibration and to minimize cross-contamination, use
two beakers for each buffer solution: one for rinsing the electrode and one for calibration. If you are
measuring in the acidic range, use pH 7.01 or 6.86 as the first buffer and pH 4.01 as the second
buffer. If you are measuring in the alkaline range, use pH 7.01 or 6.86 as the first buffer and
pH 10.01 or 9.18 as the second buffer.
Procedure
Calibration can be performed using one or two calibration buffers. For more accurate
measurements, a two-point calibration is recommended.
Submerse the pH electrode approximately 3 cm (1¼”) into a buffer solution and stir gently. From
the Probe Measurement screen, press the Calibrate key to begin the calibration process.
When the reading is stable and close to the selected buffer, the Confirm key will become available.
Press Confirm to accept and store the calibration point.
The meter will now prompt for the second buffer (“Buffer: 2”). To use only a one-point calibration,
to exit calibration mode at this time. The meter will store the calibration information
press
to the probe and return to Measurement mode. To continue calibrating with a second buffer, rinse
and submerse the pH electrode approximately 3 cm (1¼”) into the second buffer solution and stir
gently. If necessary, press
keys to select a different buffer value.
When the reading is stable and close to the selected buffer, the Confirm key will become available.
Press Confirm to accept and store the second calibration point.
The meter will store the two-point calibration information to the probe and return to Measurement
mode. The list of calibrated buffers will appear at the bottom of the screen.
24
Clean Probe:
The "Clean Probe" message indicates poor electrode
performance (offset out of accepted window, or slope under the
accepted lower limit). Often, cleaning the probe will improve
the pH electrode's response. See pH Electrode Conditioning and
Maintenance for details. Repeat calibration after cleaning.
PROBE MODE
7.2. pH CALIBRATION MESSAGES
Check Probe & Buffer:
The “Check Probe & Buffer” message appears when there
is a large difference between the pH measurement and the
selected buffer value, or the electrode slope is outside of the
accepted slope limit. You should check your probe and confirm
the correct buffer selection. Cleaning may also improve this
response.
Wrong Temperature:
The buffer temperature is too extreme for the selected buffer value.
7.3. pH MEASUREMENT
The HI83306 can be used to perform direct pH measurements by connecting a HANNA® digital
pH electrode with a 3.5 mm TRRS connector. To begin taking probe measurements, connect the
electrode to the 3.5 mm port marked with EXT PROBE located at the rear of the meter. If the meter
is in “Photometer Mode”, set the meter to “Probe Mode” by pressing the MODE key.
While taking pH probe measurements, the following functions are available:
• Calibrate: Press to access electrode calibration functions.
• GLP: Press to review the last calibration information, including date/time, buffers used, slope,
and offset.
• Range: Press to switch between “pH” units and “mV” units.
25
PROBE MODE
Press to switch to Photometer mode.
Press to access the meter’s Setup menu.
Press to log the current measurement.
Press to review the meter’s log history.
Press to view contextual help information.
For high accuracy it is recommended to calibrate your electrode often. pH electrodes should be
recalibrated at least once per week, but daily calibration is recommended. Always recalibrate after
cleaning an electrode. See page 23 for more information on pH calibration.
To take pH measurements:
• Remove the protective cap and rinse the electrode with water.
• Collect some sample in a clean, dry beaker.
• Preferably, rinse the electrode with a small amount of sample. Discard the rinse.
• Submerse the electrode tip approximately 3 cm (1¼”) into the sample to be tested and stir the
sample gently. Make sure the electrode junction is completely submersed.
symbol disappears, your
• Allow time for the electrode to stabilize in the sample. When the
reading is stable.
If measurements are taken successively in different samples, it is recommended to rinse the
electrodes thoroughly with deionized or distilled water and then with some of the next sample to
prevent cross-contamination.
pH measurements are affected by temperature. HANNA® Digital pH electrodes include a built-in
temperature sensor and automatically calculate corrected pH values. The measured temperature is
displayed on the screen with the pH measurements.
26
No Probe:
No probe is connected or the probe is broken.
Connecting:
The meter has detected a probe and is reading the probe
configuration and calibration information.
PROBE MODE
7.4. pH MEASUREMENT MESSAGES / WARNINGS
Incompatible Probe:
The connected probe is not compatible with this device.
Incompatible Calibration:
The probe’s current calibration is not compatible with this
meter. The calibration must be cleared to use this probe.
Exceeded Probe Range:
The pH and/or temperature measurement exceed the
specifications of the probe. The affected measurement value(s)
will be flashing.
Broken Temperature Sensor:
The temperature sensor inside the probe is broken.
Temperature compensation will revert to a fixed value of
25 °C (77 °C).
Cal Due:
The probe has no calibration. See section Probe Calibration.
27
PROBE MODE
7.5. pH GLP
Good Laboratory Practice (GLP) refers to a quality control function used to ensure uniformity and
consistency of sensor calibrations and measurements. To view the GLP information, press the GLP
key from the Probe Measurement screen.
The pH GLP screen displays the following information about the last pH calibration:
• Date and time of the last calibration
• List of buffers used in the last calibration
• Calculated slope and offset
• Press ESC to return in measurement mode.
28
PROBE MODE
7.6. pH ELECTRODE CONDITIONING AND MAINTENANCE
Remove the protective cap of the pH electrode.
DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT.
This is normal with electrodes. They will disappear when rinsed with water.
During transport, tiny bubbles of air may form inside the glass bulb affecting proper functioning of
the electrode. These bubbles can be removed by “shaking down” the electrode as you would do with
a glass thermometer. If the bulb and/or junction is dry, soak the electrode in HI70300 or HI80300
storage solution for at least one hour.
For refillable electrodes:
If the filling solution (electrolyte) is more than 2½ cm (1”) below the fill hole, add HI7082 or
HI8082 3.5M KCl Electrolyte Solution for double junction electrodes.
Unscrew the fill hole cover during measurements so the liquid reference junction maintains
an outward flow of electrolyte.
Measurement
Rinse the electrode tip with distilled water. Submerse the tip 3 cm (1¼”) in the sample and stir
gently for a few seconds. For a faster response and to avoid cross-contamination of the samples, rinse
the electrode tip with a few drops of the solution to be tested, before taking measurements.
29
PROBE MODE
Storage Procedure
To minimize clogging and ensure a quick response time, the glass bulb and the junction should be
kept moist and not allowed to dry out.
Replace the solution in the protective cap with a few drops of HI70300 or HI80300 Storage
Solution or, in its absence, Filling Solution (HI7082 or HI8082 for double junction electrodes).
Follow the preparation procedure before taking measurements.
Note: NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.
Periodic Maintenance
Inspect the electrode and the cable. The cable used for connection to the instrument must be intact
and there must be no points of broken insulation on the cable or cracks on the electrode stem or
bulb. Connectors must be perfectly clean and dry. If any scratches or cracks are present, replace the
electrode. Rinse off any salt deposits with water.
For refillable electrodes: Refill the reference chamber with fresh electrolyte (HI7082 or HI8082 for
double junction electrodes). Allow the electrode to stand upright for 1 hour.
Follow the Storage Procedure above.
Cleaning Procedure
Use diagnostic messages to aid pH electrode troubleshooting. Several cleaning solutions are
available:
• General – Soak in Hanna HI7061 or HI8061 General Cleaning Solution for
approximately ½ hour.
• Protein –Soak in Hanna HI7073 or HI8073 Protein Cleaning Solution for 15 minutes.
• Inorganic – Soak in Hanna HI7074 Inorganic Cleaning Solution for 15 minutes.
• Oil/grease – Rinse with Hanna HI7077 or HI8077 Oil and Fat Cleaning Solution.
Note: After performing any of the cleaning procedures, rinse the electrode thoroughly with
distilled water, refill the reference chamber with fresh electrolyte (not necessary for gel- filled
electrodes) and soak the electrode in HI70300 or HI80300 Storage Solution for at least
1 hour before taking measurements.
Temperature Correlation For pH Sensitive Glass
Verify the temperature range by reading the limits on electrodes cap. The pH electrode’s life also
depends on the temperature that is used. If constantly cycled between two temperatures, the life of
the electrode is drastically reduced.
30
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 3.00 mg/L (as NH3-N)
0.01 mg/L
±0.04 mg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 420 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D1426 Nessler method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93700A-0
Ammonia Low Range Reagent A
4 drops
HI93700B-0
Ammonia Low Range Reagent B
4 drops
AMMONIA LOW RANGE
8. METHOD PROCEDURES
8.1. AMMONIA LOW RANGE
REAGENT SETS
HI93700-01
Reagents for 100 tests
HI93700-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Ammonia LR method using the procedure described in the
Method Selection section (see page 17).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
31
AMMONIA LOW RANGE
• Add 4 drops of HI93700A-0 Ammonia Low Range Reagent A.
Replace the cap and mix the solution.
• Add 4 drops of HI93700B-0 Ammonia Low Range Reagent B.
Replace the cap and mix the solution.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L of ammonia nitrogen (NH3-N).
32
• Press  or  to return to the measurement screen.
AMMONIA LOW RANGE
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of ammonia (NH3) and ammonium
(NH4+).
INTERFERENCE
Interference may be caused by:
Acetone
Alcohols
Aldehydes
Glycine
Hardness above 1 g/L
Iron
Organic chloramines
Sulfide
Various aliphatic and aromatic amines
33
AMMONIA MEDIUM RANGE
8.2. AMMONIA MEDIUM RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 10.00 mg/L (as NH3-N)
0.01 mg/L
±0.05 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 420 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D1426, Nessler method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93715A-0
Ammonia Medium Range Reagent A
4 drops
HI93715B-0
Ammonia Medium Range Reagent B
4 drops
REAGENT SETS
HI93715-01
Reagents for 100 tests
HI93715-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Ammonia MR method using the procedure described in the
Method Selection section (see page 17).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
34
• Add 4 drops of HI93715A-0 Ammonia Medium Range
Reagent A. Replace the cap and mix the solution.
• Add 4 drops of HI93715B-0 Ammonia Medium Range
Reagent B. Replace the cap and mix the solution.
AMMONIA MEDIUM RANGE
• Remove the cuvette.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results to mg/L of ammonia nitrogen (NH3-N).
• Press or to access the second level functions.
35
AMMONIA MEDIUM RANGE
36
• Press the Chem Frm key to convert the result in mg/L of ammonia (NH3) and ammonium
(NH4+).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Acetone
Alcohols
Aldehydes
Glycine
Hardness above 1 g/L
Iron
Organic chloramines
Sulfide
Various aliphatic and aromatic amines
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 100.0 mg/L (as NH3-N)
0.1 mg/L
±0.5 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 420 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D1426, Nessler method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93733A-0
Ammonia High Range Reagent A
4 drops
HI93733B-0
Ammonia High Range Reagent B
9 mL
AMMONIA HIGH RANGE
8.3. AMMONIA HIGH RANGE
REAGENT SETS
HI93733-01
Reagents for 100 tests
HI93733-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Ammonia HR method using the procedure described in the
Method Selection section (see page 17).
1 mL
• Add 1mL of unreacted sample to the cuvette using 1mL
syringe.
• Use the pipette to fill the cuvette up to the 10 mL mark
with HI93733B-0 Ammonia High Range Reagent B.
Replace the cap and mix the solution.
• Place the cuvette into the holder and close the lid.
37
AMMONIA HIGH RANGE
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Add 4 drops of HI93733A-0 Ammonia High Range Reagent A.
Replace the cap and swirl the solution.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L of ammonia nitrogen (NH3-N).
• Press  or  to access the second level functions.
38
•Press  or  to return to the measurement screen.
AMMONIA HIGH RANGE
•Press the Chem Frm key to convert the result to mg/L of ammonia (NH3) and ammonium
(NH4+).
INTERFERENCES
Interference may be caused by:
Acetone
Alcohols
Aldehydes
Glycine
Hardness above 1 g/L
Iron
Organic chloramines
Sulfide
Various aliphatic and aromatic amines
39
CHLORINE, FREE
8.4. CHLORINE, FREE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 5.00 mg/L (as Cl2)
0.01 mg/L
±0.03 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the EPA DPD method 330.5.
REQUIRED REAGENTS
POWDER:
CodeDescriptionQuantity
HI93701-0
Free Chlorine Reagent
1 packet
LIQUID:
CodeDescriptionQuantity
HI93701A-F
Free Chlorine Reagent A
3 drops
HI93701B-F
Free Chlorine Reagent B
3 drops
REAGENT SETS
HI93701-F
Reagents for 300 tests (liquid)
HI93701-01
Reagents for 100 tests (powder)
HI93701-03
Reagents for 300 tests (powder)
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Chlorine (Free) method using the procedure described in
the Method Selection section (see page 17).
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
40
10 mL
CHLORINE, FREE
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
POWDER REAGENT PROCEDURE
• Add the content of one packet of HI93701-0 Free Chlorine
Reagent. Replace the cap and shake gently for 20 seconds.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or alternatively,
wait for 1 minute and press Read. When the timer ends the meter will perform the reading. The
instrument displays the results in mg/L of chlorine (Cl2).
LIQUID REAGENT PROCEDURE
• To an empty cuvette add 3 drops of HI93701A-F Free
Chlorine Reagent A and 3 drops of HI93701B-F Free
Chlorine Reagent B.
41
CHLORINE, FREE
• Swirl gently to mix.
• Add 10 mL of unreacted sample (up to the mark).
Replace the cap and shake gently.
10 mL
• Insert the cuvette into the instrument and close the lid.
• Press Read to start the reading. The instrument displays the results in mg/L of chlorine (Cl2).
Note: Free and Total Chlorine have to be measured separately with fresh sample following
the related procedure if both values are desired.
INTERFERENCES
Interference may be caused by: Bromine, Iodine, Ozone, Oxidized forms of Chromium and
Manganese. In case of water with hardness greater than 500 mg/L CaCO3, shake the sample for
approximately 2 minutes after adding the powder reagent.
If the water used for this procedure has an alkalinity value greater than 250 mg/L CaCO3 or acidity
value greater than 150 mg/L CaCO3, the color of the sample may develop only partially, or may
rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH.
42
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
CHLORINE, TOTAL
8.5. CHLORINE, TOTAL
0.00 to 5.00 mg/L (as Cl2)
0.01 mg/L
±0.03 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the EPA DPD method 330.5.
REQUIRED REAGENTS
POWDER:
CodeDescriptionQuantity
HI93711-0
Total Chlorine Reagent
1 packet
LIQUID:
CodeDescriptionQuantity
HI93701A-T
Total Chlorine Reagent A
3 drops
HI93701B-T
Total Chlorine Reagent B
3 drops
HI93701C-T
Total Chlorine Reagent C
1 drop
REAGENT SETS
HI93701-T
Reagents for 300 tests (liquid)
HI93711-01
Reagents for 100 total tests (powder)
HI93711-03
Reagents for 300 total tests (powder)
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Chlorine (Total) method using the procedure described in
the Method Selection section (see page 17).
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
43
CHLORINE, TOTAL
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
POWDER REAGENT PROCEDURE
• Add 1 packet of HI93711-0 Total Chlorine Reagent. Replace the
cap and shake gently for 20 seconds.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L of chlorine (Cl2).
LIQUID REAGENT PROCEDURE
• To an empty cuvette add 3 drops of HI93701A-T
Total Chlorine Reagent A, 3 drops of HI93701B-T
Total Chlorine Reagent B, and 1 drop of
HI93701C-T Total Chlorine Reagent C. Swirl gently
to mix.
44
10 mL
CHLORINE, TOTAL
• Add 10 mL of unreacted sample (up to the mark).
Replace the cap and shake gently.
• Insert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L of chlorine (Cl2).
Note: Free and Total Chlorine have to be measured separately with fresh unreacted samples
following the related procedure if both values are desired.
INTERFERENCES
Interference may be caused by: Bromine, Iodine, Ozone, Oxidized forms of Chromium and
Manganese. In case of water with hardness greater than 500 mg/L CaCO3 shake the sample for
approximately 2 minutes after adding the powder reagent.
If the water used for this procedure has an alkalinity value greater than 250 mg/L CaCO3 or acidity
value greater than 150 mg/L CaCO3, the color of the sample may develop only partially, or may
rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH.
45
CHROMIUM (VI) LOW RANGE
8.6. CHROMIUM (VI) LOW RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 300 µg/L (as Cr (VI))
1 µg/L
±10 µg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D1687 Diphenylcarbohydrazide method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93749-0
Chromium (VI) Low Range Reagent
1 packet
REAGENT SETS
HI93749-01
Reagents for 100 tests
HI93749-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Chromium (VI) LR method using the procedure described
in the Method Selection section (see page 17).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Add one packet of HI93749-0 Chromium(VI) Low Range
Reagent. Replace the cap and shake vigorously for about
10 seconds.
46
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 6 minutes and press Read. When the timer ends the meter will perform the reading. The
instrument displays concentration in µg/L of chromium (Cr6+).
CHROMIUM (VI) LOW RANGE
• Reinsert the cuvette into the instrument and close the lid.
• Press or  to access the second level functions.
• Press the Chem Frm key to convert the result to µg/L of chromate (CrO42¯) and dichromate
(Cr2O72¯).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by: Vanadium above 1 ppm. However, waiting 10 minutes before
reading removes the interference. Iron above 1 ppm. Mercurous and mercuric ions cause slight
inhibition of the reaction.
47
CHROMIUM (VI) HIGH RANGE
8.7. CHROMIUM (VI) HIGH RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 1000 µg/L (as Cr(VI))
1 µg/L
±5 µg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D1687, Diphenylcarbohydrazide method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93723-0
Chromium(VI) High Range Reagent
1 packet
REAGENT SETS
HI93723-01
Reagents for 100 tests
HI93723-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Chromium (VI) HR method using the procedure described
in the Method Selection section (see page 17).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Add one packet of HI93723-0 Chromium(VI) High Range
Reagent. Replace the cap and shake vigorously for about
10 seconds.
48
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 6 minutes and press Read. When the timer ends the meter will perform the reading. The
instrument displays concentration in µg/L of chromium (Cr6+).
CHROMIUM (VI) HIGH RANGE
• Reinsert the cuvette into the instrument and close the lid.
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to µg/L of chromate (CrO42¯) and dichromate
(Cr2O72¯).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Vanadium above 1 ppm. However, waiting 10 minutes before reading removes the interference.
Iron above 1 ppm.
Mercurous and mercuric ions cause slight inhibition of the reaction.
49
COLOR OF WATER
8.8. COLOR OF WATER
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 500 PCU (Platinum Cobalt Units)
1 PCU
±10 PCU ±5% of reading at 25 °C
LED with narrow band interference filter @ 420 nm
Adaptation of the Standard Methods for the Examination of Water and
Wastewater, 18th edition, Colorimetric Platinum Cobalt method.
REQUIRED ACCESSORIES
0.45 µm membrane for true color measurement.
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Color of Water method using the procedure described in
the Method Selection section (see page 17).
10 mL
• Fill the first cuvette (#1) up to the mark with deionized water and
replace the cap.
#1
#1
• Place the blank (#1) into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette
50
10 mL
#2
• Filter 10 mL of sample through a filter with a 0.45 µm
membrane into the third cuvette (#3), up to the 10 mL
mark and replace the cap. This is the true color.
• Insert the apparent color cuvette (#2) into the instrument
and close the lid.
#3
COLOR OF WATER
• Fill the second cuvette (#2) up to the mark with unfiltered
sample and replace the cap. This is the apparent color.
#2
• Press Read to start the reading. The meter displays the value of apparent color in Platinum
Cobalt Units (PCU).
• Remove the apparent color cuvette(#2) from the
instrument, insert the true color cuvette (#3) into the
instrument and close the lid.
#3
•Press Read to start the reading. The meter displays the true color in Platinum Cobalt Units
(PCU).
51
COPPER LOW RANGE
8.9. COPPER LOW RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.000 to 1.500 mg/L (as Cu)
0.001 mg/L
±0.010 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the EPA method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI95747-0
Copper Low Range Reagent
1 packet
REAGENT SETS
HI95747-01
Reagents for 100 tests
HI95747-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Copper LR method using the procedure described in the
Method Selection section (see page 17).
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
52
• Add one packet of HI95747-0 Copper Low Range Reagent.
Replace the cap and shake gently for about 15 seconds.
• Reinsert the cuvette into the instrument and close the lid.
COPPER LOW RANGE
• Remove the cuvette.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 45 seconds and press Read. When the timer ends the meter will perform the reading.
The instrument displays the results in mg/L of copper (Cu).
INTERFERENCES
Interference may be caused by:
Silver, Cyanide.
For samples overcoming buffering capacity of reagent (around pH 6.8), pH should be adjusted
between 6 and 8.
53
COPPER HIGH RANGE
8.10. COPPER HIGH RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 5.00 mg/L (as Cu)
0.01 mg/L
±0.02 mg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the EPA method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93702-0
Copper High Range Reagent
1 packet
REAGENT SETS
HI93702-01
Reagents for 100 tests
HI93702-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Copper HR method using the procedure described in the
Method Selection section (see page 17).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
54
• Add one packet of HI93702-0 Copper High Range Reagent.
Replace the cap and shake gently for about 15 seconds.
• Reinsert the cuvette into the instrument and close the lid.
COPPER HIGH RANGE
• Remove the cuvette.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 45 seconds and press Read. When the timer ends the meter will perform the reading.
The instrument displays the results in mg/L of copper (Cu).
INTERFERENCES
Interference may be caused by:
Silver, Cyanide.
For samples overcoming buffering capacity of reagent (around pH 6.8), pH should be adjusted
between 6 and 8.
55
CYANURIC ACID
8.11. CYANURIC ACID
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 80 mg/L (as CYA)
1 mg/L
±1 mg/L ±15% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the turbidimetric method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93722-0
Cyanuric Acid Reagent
1 packet
REAGENT SETS
HI93722-01
Reagents for 100 tests
HI93722-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Cyanuric Acid method using the procedure described in the
Method Selection section (see page 17).
• Fill the first cuvette with 10 mL of unreacted
sample (up to the mark) and replace the cap.
#1
10 mL
#1
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
56
CYANURIC ACID
• Fill a beaker with 25 mL sample (up to the mark).
• Add one packet of HI93722-0 Cyanuric Acid Reagent and
mix to dissolve.
#2
• Fill a second cuvette with 10 mL of the reacted sample,
up to the mark, and replace the cap.
• Reinsert the cuvette into the instrument and close the lid.
10 mL
#2
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 45 seconds and press Read. When the timer ends the meter will perform the reading.
The instrument displays the concentration in mg/L of cyanuric acid.
57
MOLYBDENUM
8.12. MOLYBDENUM
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 40.0 mg/L (as Mo6+)
0.1 mg/L
±0.3 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 420 nm
Adaptation of the mercaptoacetic acid method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93730A-0
Molybdenum Reagent A
1 packet
HI93730B-0
Molybdenum Reagent B
1 packet
HI93730C-0
Molybdenum Reagent C
1 packet
REAGENT SETS
HI93730-01
Reagents for 100 tests
HI93730-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Molybdenum method using the procedure described in the
Method Selection section (see page 17).
• Fill the cuvette with 10 mL of unreacted sample (up to the
mark) and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
58
25 mL
MOLYBDENUM
• Fill one graduated mixing cylinder up to the 25 mL mark
with the sample.
• Add one packet of HI93730A-0 Molybdenum Reagent A,
close the cylinder and invert several times until completely
dissolved.
• Add one packet of HI93730B-0 Molybdenum Reagent B to
the cylinder, close and invert several times until completely
dissolved.
• Add one packet of HI93730C-0 Molybdenum Reagent C to
the cylinder, close and shake vigorously.
• Fill an empty cuvette with 10 mL of reacted sample (up to
the mark) and replace the cap.
10 mL
• Insert the cuvette into the instrument and close the lid.
59
MOLYBDENUM
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 5 minutes and press Read. When the timer ends the meter will perform the reading. The
instrument displays concentration in mg/L of molybdenum (Mo6+).
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of molybdate (MoO42-) and sodium
molybdate (Na2MoO4).
• Press or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Aluminum above 50 mg/L
Chromium above 1000 mg/L
Copper above 10 mg/L
Iron above 50 mg/L
Nickel above 50 mg/L
Nitrite, as NO2¯
Sulfate above 200 mg/L
Highly buffered samples or with extreme pH may exceed the buffering capacity of the reagents
60
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.000 to 1.000 mg/L (as Ni)
0.001 mg/L
±0.010 mg/L ±7% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the PAN method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93740A-0
Nickel Low Range Reagent A
2 packets
HI93740B-0
Nickel Low Range Reagent B
2 mL
HI93740C-0
Nickel Low Range Reagent C
2 packets
HI93703-51
Dispersing Agent (optional reagent)
4-6 drops
NICKEL LOW RANGE
8.13. NICKEL LOW RANGE
REAGENT SETS
HI93740-01
Reagents for 50 tests
HI93740-03
Reagents for 150 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Nickel LR method using the procedure described in the
Method Selection section (see page 17).
Note: For best results samples should be between 20 and 24 °C.
• Fill one graduated beaker with 25 mL of deionized water (blank)
and another one with 25 mL of sample.
• Add one packet of HI93740A-0 Nickel Low Range Reagent A to each
beaker. Cap and swirl gently until the reagent is dissolved.
Note: If sample contains iron (Fe3+), it is important that all
powder is dissolved before continuing.
• Add 1 mL of HI93740B-0 Nickel Low Range Reagent B to each
beaker, and swirl to mix.
61
NICKEL LOW RANGE
• Press Timer and the display will show a countdown or alternatively, wait for 15 minutes.
•Add one packet of HI93740C-0 Nickel Low Range Reagent C
to each beaker, cap and swirl to mix until completely dissolved.
10 mL
•Fill one cuvette (#1) with 10 mL of the blank (up to the mark).
• Place the cuvette into the holder and close the lid.
#1
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
10 mL
• Fill a second cuvette (#2) with 10 mL of the reacted sample
(up to the mark).
10 mL
#2
• Insert the second cuvette into the instrument and close the lid.
62
Note: A temperature above 30 °C may cause turbidity. In this case add 2-3 drops of
HI93703-51 Dispersing Agent to each cuvette and swirl until turbidity is removed before
zeroing the meter and reading the sample.
NICKEL LOW RANGE
• Press Read to start the reading. The instrument displays the results in mg/L of nickel (Ni).
INTERFERENCES
Interference may be caused by:
Co2+ must not be present
Fe2+ must not be present
Al3+ above 32 mg/L
Ca2+ above 1000 mg/L (as CaCO3)
Cd2+ above 20 mg/L
Cl¯ above 8000 mg/L
Cr3+ above 20 mg/L
Cr6+ above 40 mg/L
Cu2+ above 15 mg/L
F¯ above 20 mg/L
Fe3+ above 10 mg/L
K+ above 500 mg/L
Mg2+ above 400 mg/L
Mn2+ above 25 mg/L
Mo6+ above 60 mg/L
Na+ above 5000 mg/L
Pb2+ above 20 mg/L
Zn2+ above 30 mg/L
63
NICKEL HIGH RANGE
8.14. NICKEL HIGH RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 7.00 g/L (as Ni)
0.01 g/L
±0.07g/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the photometric method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93726-0
Nickel High Range Reagent
1 packet
REAGENT SETS
HI93726-01
Reagents for 100 tests
HI93726-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Nickel HR method using the procedure described in the
Method Selection section (see page 17).
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
64
Remove the cuvette and add one packet of HI93726-0
Nickel High Range Reagent. Replace the cap and shake
gently until completely dissolved.
• Reinsert the cuvette into the instrument and close the lid.
NICKEL HIGH RANGE
•
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 1 minute and press Read. When the timer ends the meter will perform the reading. The
instrument displays the concentration in g/L of nickel (Ni).
INTERFERENCES
Interference may be caused by copper.
65
NITRATE
8.15. NITRATE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 30.0 mg/L (as NO3-- N)
0.1 mg/L
±0.5 mg/L ±10% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the cadmium reduction method.
REQUIRED REAGENTS
Code
Description
HI93728-0
Nitrate Reagent
Quantity
1 packet
REAGENT SETS
HI93728-01
Reagents for 100 tests
HI93728-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Nitrate method using the procedure described in
the Method Selection section (see page 17).
• Fill the cuvette with 10 mL of sample, (up to the mark),
and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
66
NITRATE
• Remove the cuvette and add one packet of HI93728-0 Nitrate Reagent.
• Replace the cap and shake vigorously up and down for exactly
10 seconds. Continue to mix by inverting the cuvette gently for
50 seconds, while taking care not to induce air bubbles. Powder
will not completely dissolve. Time and method of shaking could
sensitively affect the measurement.
Note: The method is technique-sensitive. See procedure
on page 19 Cuvette Preparation for proper mixing
technique.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 4 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L of nitrate-nitrogen (NO3-N).
• Press  or  to access the second level functions.
67
NITRATE
• Press the Chem Frm key to convert the result to mg/L of nitrate (NO3¯).
•Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Ammonia and amines, as urea and primary aliphatic amines
Chloride above 100 ppm
Chlorine above 2 ppm
Copper
Iron(III)
Strong oxidizing and reducing substances
Sulfide must be absent
68
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 150 mg/L (as NO2-)
1 mg/L
±4 mg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the Ferrous Sulfate method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93708-0
Nitrite High Range Reagent
1 packet
NITRITE HIGH RANGE
8.16. NITRITE HIGH RANGE
REAGENT SETS
HI93708-01
Reagents for 100 tests
HI93708-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Nitrite HR method using the procedure described
in the Method Selection section (see page 17).
10 mL
• Fill the cuvette up to the mark with 10 mL of unreacted
sample (up to the mark) and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
69
NITRITE HIGH RANGE
• Add one packet of HI93708-0 Nitrite High Range Reagent.
Replace the cap and shake gently until completely
dissolved.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 10 minutes and press Read. When the timer ends the meter will perform the reading.
The instrument displays concentration in mg/L of nitrite (NO2-).
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of nitrite-nitrogen (NO2--N) and
sodium nitrite (NaNO2).
• Press  or  to return to the measurement screen.
70
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 10.0 mg/L (as O2)
0.1 mg/L
±0.4 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 420 nm
Adaptation of the Standard Methods for the Examination of Water and
Wastewater, 18th edition, Azide modified Winkler method.
OXYGEN, DISSOLVED
8.17. OXYGEN, DISSOLVED
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93732A-0
Dissolved Oxygen Reagent A
5 drops
HI93732B-0
Dissolved Oxygen Reagent B
5 drops
HI93732C-0
Dissolved Oxygen Reagent C
10 drops
REAGENT SET
HI93732-01
Reagents for 100 tests
HI93732-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Oxygen (dissolved) method using the procedure
described in the Method Selection section (see page 17).
• Fill one 60 mL glass bottle completely with the unreacted
sample.
• Replace the cap and ensure that a small part of the sample
spills over.
• Remove the cap and add 5 drops of HI93732A-0 and 5 drops of
HI93732B-0.
• Add more sample, to fill the bottle completely. Replace the cap
and ensure that a part of the sample spills over.
Note: This ensures no air bubbles have been trapped inside
the bottle. Trapped air bubbles could alter readings.
• Invert the bottle several times until the sample turns orangeyellow and a flocculating agent appears.
71
OXYGEN, DISSOLVED
• Let the sample stand for approximately 2 minutes to allow flocculating
agent to settle.
• When the upper half of the bottle is clear, add 10 drops of
HI93732C-0 Dissolved Oxygen Reagent C.
• Replace the cap and invert the bottle until the settled flocculating
agent dissolves completely. The sample is ready for measurement
when it is yellow and completely clear.
• Fill the first cuvette (#1) with 10 mL of the unreacted sample
(up to the mark), and replace the cap.
• Place the cuvette into the holder and close the lid.
10 mL
#1
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Fill second cuvette (#2) with 10 mL of the reacted sample
(up to the mark) and replace the cap.
72
• Insert the cuvette into the holder and close the lid.
• Press Read to start the reading. The instrument will display the results in mg/L of oxygen (O2).
OXYGEN, DISSOLVED
#2
INTERFERENCES
Interferences may be caused by reducing and oxidizing materials.
73
pH
8.18. pH
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
6.5 to 8.5 pH
0.1 pH
±0.1 pH at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the Phenol Red method.
REQUIRED REAGENTS
Code
Description
HI 93710-0
pH Reagent
Quantity
5 drops
REAGENT SETS
HI 93710-01
Reagents for 100 pH tests
HI 93710-03
Reagents for 300 pH tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the pH method using the procedure described in
the Method Selection section (see page 17).
• Fill the cuvette with 10 mL of unreacted sample (up to
the mark) and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
74
pH
• Remove the cuvette and add 5 drops of HI93710-0
pH Reagent Indicator. Replace the cap and mix the
solution.
• Reinsert the cuvette into the instrument and close the lid.
• Press the Read key to start the reading. The instrument displays the result in pH.
75
PHOSPHATE LOW RANGE
8.19. PHOSPHATE LOW RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 2.50 mg/L (as PO43-)
0.01 mg/L
±0.04 mg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 610 nm
Adaptation of the Ascorbic Acid method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93713-0
Phosphate Low Range Reagent 1 packet
REAGENT SETS
HI93713-01
Reagents for 100 tests
HI93713-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
10 mL
• Select the Phosphate LR method using the procedure
described in the Method Selection section (see page 17).
• Rinse, cap and shake the cuvette several times with
unreacted sample.
• Fill the cuvette with 10 mL of sample (up to the mark) and
replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette and add the content of one packet
of HI93713-0 Phosphate Low Range Reagent. Replace
the cap and shake gently (for about 2 minutes) until
the powder is completely dissolved.
76
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 3 minutes and press Read. When the timer ends the meter will perform the reading. The
instrument displays concentration in mg/L of phosphate (PO43¯).
PHOSPHATE LOW RANGE
• Reinsert the cuvette into the instrument and close the lid.
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of phosphorus (P) and phosphorus
pentoxide (P2O5).
• Press or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Iron above 50 mg/L
Silica above 50 mg/L
Silicate above 10 mg/L
Copper above 10 mg/L
Hydrogen sulfide, arsenate, turbid sample and highly buffered samples also interfere.
77
PHOSPHATE HIGH RANGE
8.20. PHOSPHATE HIGH RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 30.0 mg/L (as PO43-)
0.1 mg/L
±1.0 mg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the Standard Methods for the Examination of Water and
Wastewater, 18th edition, Amino Acid method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93717A-0
Phosphate High Range Reagent A 10 drops
HI93717B-0
Phosphate High Range Reagent B
1 packet
REAGENT SETS
HI93717-01
Reagents for 100 tests
HI93717-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Phosphate HR method using the
procedure described in the Method Selection
section (see page 17).
• Fill the cuvette with 10 mL of unreacted sample
(up to the mark) and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.
• Add 10 drops of HI93717A-0 Phosphate High Range Reagent A.
78
• Reinsert the cuvette into the instrument and close the lid.
PHOSPHATE HIGH RANGE
• Add one packet of HI93717B-0 Phosphate HR Reagent
B to the cuvette. Replace the cap and shake gently
until completely dissolved.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 5 minutes and press Read. When the timer ends the meter will perform the reading. The
instrument displays the results in mg/L of phosphate (PO43¯).
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of phosphorus (P) and phosphorus
pentoxide (P2O5).
• Press  or  to return to the measurement screen.
79
PHOSPHATE HIGH RANGE
80
INTERFERENCES
Sulfide
Chloride above 150000 mg/L
Calcium above 10000 mg/L as CaCO3
Magnesium above 40000 mg/L as CaCO3
Ferrous iron above 100 mg/L
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 2.00 mg/L (as SiO2)
0.01 mg/L
±0.03 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 610 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D859, Heteropoly Molybdenum Blue method.
SILICA LOW RANGE
8.21. SILICA LOW RANGE
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93705A-0
Silica Low Range Reagent A
6 drops
HI93705B-0
Silica Low Range Reagent B
1 packet
HI93705C-0
Silica Low Range Reagent C
1 packet
REAGENT SETS
HI93705-01
Reagents for 100 tests
HI93705-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Silica LR method using the procedure described
in the Method Selection section (see page 17).
• Fill the cuvette with 10 mL of unreacted sample
(up to the mark).
• Add 6 drops of HI93705A-0 Silica Low Range
Reagent A. Replace the cap and swirl the solution.
10 mL
• Press Timer and the display will show the
countdown prior to adding HI93705B-0 Silica Low
Range Reagent B, or alternatively wait 4 minutes.
• Add one packet of HI93705B-0 Silica Low Range
Reagent B and shake until it is completely dissolved.
• Press Continue and the display will show the countdown,
or alternatively wait 1 minute.
81
SILICA LOW RANGE
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Add one packet of HI93705C-0 Silica Low Range Reagent C
and shake until it is completely dissolved.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement, or alternatively,
wait 3 minutes and press Read. When the timer ends the meter will perform the reading. The
instrument displays result in mg/L of silica (SiO2).
•Press  or  to access the second level functions.
82
• Press  or  to return to the measurement screen.
SILICA LOW RANGE
• Press the Chem Frm key to convert the result to mg/L of silicon (Si).
INTERFERENCES
Interference may be caused by:
Phosphate above 60 mg/L (causes a 2% reduction in reading)
Phosphate above 75 mg/L (causes an 11% reduction in reading)
Sulfide and high concentration of iron
Eliminate color and turbidity interferences by zeroing the meter with the original water sample.
83
SILVER
8.22. SILVER
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.000 to 1.000 mg/L (as Ag)
0.001 mg/L
±0.020 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 575 nm.
Adaptation of the PAN method.
REQUIRED REAGENTS
Code
Description
HI93737A-0
Silver Reagent A
HI93737B-0
Silver Reagent B
HI93737C-0
Silver Reagent C
HI93737D-0
Silver Reagent D
HI93703-51
Dispersing Agent
Quantity
1 mL
1 mL
2 mL
2 mL
6 drops
REAGENT SETS
HI93737-01
Reagents for 50 tests
HI93737-03
Reagents for 150 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Silver method using the procedure described in the Method
Selection section (see page 17).
Note: For best results perform your tests between 20-24 °C.
• Fill two graduated beakers with 25 mL of sample.
• Add 1 mL of HI93737A-0 Silver Reagent A to beaker #1 (the
blank) and swirl gently to mix.
84
SILVER
• Add 1mL of HI93737B-0 Silver Reagent B to beaker #2
(the sample) and swirl gently to mix.
• Press Timer and the display will show the countdown prior to adding HI93737C-0 Silver Reagent
C or alternatively, wait for 2 minutes.
• Add 1 mL of HI93737C-0 Silver Reagent C to each beaker
and swirl.
•Press Continue and the display will show the countdown prior to adding HI93737D-0 Silver
Reagent D, or alternatively wait for 2 minutes.
• Add 1 mL of HI93737D-0 Silver Reagent D to each beaker
and swirl.
85
SILVER
• Press Continue and the display will show the countdown or alternatively wait for 2 minutes.
#1
• Fill cuvette (#1) up with 10 mL of the blank (up to the mark).
10 mL
• Add 3 drops of HI93703-51 Dispersing Agent, replace the
cap and invert gently for 10 seconds.
#1
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Fill a second cuvette (#2) up with 10 mL of the reacted sample
(up to the mark).
86
10 mL
#2
SILVER
• Add 3 drops of HI93703-51 Dispersing Agent, replace
the cap and invert gently for 10 seconds.
• Insert the second cuvette (#2) into the instrument.
#2
• Press Read to start the reading. The instrument displays the results in mg/L of silver (Ag).
INTERFERENCES
Interference may be caused by:
Al3+ above 30 mg/L
Ca2+ above 1000 mg/L (as CaCO3)
Cd2+ above 20 mg/L
Cl¯ above 8000 mg/L
Co2+ above 1.5 mg/L
Cr3+ above 20 mg/L
Cr6+ above 40 mg/L
Cu2+ above 15 mg/L
F¯ above 20 mg/L
Fe2+ above 1.5 mg/L
Fe3+ above 10 mg/L
K+ above 500 mg/L
Mn2+ above 25 mg/L
Mg2+ above 1000 mg/L (as CaCO3)
Na+ above 5000 mg/L
Ni2+ above 1.5 mg/L
Pb2+ above 20 mg/L
Zn2+ above 30 mg/L
87
ZINC
8.23. ZINC
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 3.00 mg/L (as Zn)
0.01 mg/L
±0.03 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the Standard Methods for the Examination of Water and
Wastewater, 18th edition, Zincon method.
REQUIRED REAGENT
Code
Description
HI93731A-0
Zinc Reagent A
HI93731B-0
Zinc Reagent B
Quantity
1 packet
0.5 mL
REAGENT SETS
HI93731-01
Reagents for 100 tests
HI93731-03
Reagents for 300 tests
For other accessories see page 97.
MEASUREMENT PROCEDURE
• Select the Zinc method using the procedure described in the Method
Selection section (see page 17).
• Fill the graduated glass vial up to the 20 mL mark with the sample.
20 mL
• Add one packet of HI93731A-0 Zinc Reagent A, close the
cylinder, and invert several times to mix until completely
dissolved.
• Fill a cuvette with 10 mL of the reacted sample (up to the
mark) and close the cap.
10 mL
88
ZINC
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready
for measurement.
• Add 0.5 mL of HI93731B-0 Zinc Reagent B to the cuvette,
close the cuvette with the supplied HDPE plastic stopper to
prevent contamination.
• Replace the cap and mix for 15 seconds.
• Insert the sample into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or,
alternatively, wait for 3 minutes and 30 seconds and press Read. When the timer ends the
meter will perform the reading. The instrument displays the results in mg/L of zinc (Zn).
89
ZINC
INTERFERENCES
Interference may be caused by:
Aluminum above 6 mg/L Iron above 7 mg/L
Cadmium above 0.5 mg/L Manganese above 5 mg/L
Copper above 5 mg/L Nickel above 5 mg/L
90
Explanation: There is an excess amount of ambient light reaching the
detector.
Recommended action: Make sure the lid is closed before performing
any measurements. If the issue persists, please contact Hanna
Instruments technical support.
WARNINGS & ERRORS
9. WARNINGS & ERRORS
The instrument shows clear warning messages when erroneous conditions appear and when measured
values are outside the expected range. The information below provides an explanation of the errors
and warnings, and recommended action to be taken.
Explanation: The sample and the Zero cuvettes are inverted.
Recommended action: Swap the cuvettes and repeat the measurement.
Explanation: There is either too much light or the instrument can not
adjust the light level.
Recommended action: Please check the preparation of the Zero
cuvette and that the sample does not contain any debris.
Explanation: The meter is either overheating or its temperature has
dropped too low to operate within published accuracy specifications.
Recommended action: Allow the meter to reach normal environmental
temperature before performing any measurements.
Explanation: Meter temperature has changed significantly since the
zero measurement has been performed.
Recommended action: The zero measurement must be performed
again.
Explanation: The measured value is outside the limits of the method.
Recommended action: If possible, change the method range. Verify
that the sample does not contain any debris. Check the sample
preparation and the measurement preparation.
91
WARNINGS & ERRORS
Explanation: The measured value cannot be calculated.
Recommended action: Please check sample preparation and
measurement procedure.
Explanation: Stored results of the CAL Check measurements have been
lost.
Recommended action: Please redo the CAL Check measurements to
ensure accurate results.
Explanation: User settings have been lost.
Recommended action: Please reset the values. If the issue persists,
please contact Hanna Instruments technical support.
Explanation: Flash drive is not recognized or it might be damaged.
Recommended action: Please insert a new USB flash drive.
Explanation: Data log is full.
Recommended action: Please review logged data and delete
unnecessary logs.
Explanation: Date and time settings have been lost.
Recommended action: Please reset the values. If the issue persists,
please contact Hanna Instruments technical support.
Explanation: Battery level is too low to ensure normal functioning and
the meter will turn off.
Recommended action: Connect the USB adapter to charge the battery.
92
Explanation: English is the only available language. Help function is
not available.
Explanation: Real time clock it's not accurate.
WARNINGS & ERRORS
The instrument shows warning messages when some of the features become unavailable. To recover
them follow the Recommended action: Restart the meter. If the issue persists, please contact Hanna
Instruments technical support.
Explanation: The device serial number inside the memory can not be
identified.
Explanation: Logged data are no longer accessible.
Explanation: Battery charge level is not accurate.
If a critical error appears, below message is displayed.
Explanation: A critical error has occured.
Recommended action: Restart the meter. If the issue persists, please
contact Hanna Instruments technical support.
93
STANDARD METHODS
94
10. STANDARD METHODS
Description
Range
Method
Ammonia LR
Ammonia MR
Ammonia HR
Chlorine, Free LR
Chlorine, Total LR
Chromium (VI) LR
Chromium (VI) HR
Color of Water
Copper LR
Copper HR
Cyanuric Acid Molybdenum
Nickel LR
Nickel HR
Nitrate Nitrite HR
Oxygen, Dissolved pH
Phosphate LR
Phosphate HR
Silica LR
Silver Zinc
0.00 to 3.00 mg/L
0.00 to 10.00 mg/L
0.0 to 100.0 mg/L
0.00 to 5.00 mg/L
0.00 to 5.00 mg/L
0 to 300 µg/L
0 to 1000 µg/L
0 to 500 PCU
0.000 to 1.500 mg/L
0.00 to 5.00 mg/L
0 to 80 mg/L
0.0 to 40.0 mg/L
0.000 to 1.000 mg/L
0.00 to 7.00 g/L
0.0 to 30.0 mg/L
0 to 150 mg/L
0.0 to 10.0 mg/L
6.5 to 8.5 pH
0.00 to 2.50 mg/L
0.0 to 30.0 mg/L
0.00 to 2.00 mg/L
0.000 to 1.000 mg/L
0.00 to 3.00 mg/L
Nessler
Nessler
Nessler
DPD
DPD
Diphenylcarbohydrazide
Diphenylcarbohydrazide
Colorimetric Platinum Cobalt
Bicinchoninate
Bicinchoninate
Turbidimetric
Mercaptoacetic Acid
PAN
Colorimetric
Cadmium reduction
Ferrous Sulfate
Winkler
Phenol Red
Ascorbic Acid
Amino Acid
Heteropoly Blue
PAN
Zincon
Description
100 ammonia LR tests
300 ammonia LR tests
100 chlorine free tests (powder)
300 chlorine free tests (powder)
300 chlorine free tests (liquid)
300 chlorine total tests (liquid)
100 copper HR tests
300 copper HR tests
100 silica LR tests
300 silica LR tests
100 nitrite HR tests
300 nitrite HR tests
100 pH tests
300 pH tests
100 chlorine total tests (powder)
300 chlorine total tests (powder)
100 phosphate LR tests
300 phosphate LR tests
100 ammonia MR tests
300 ammonia MR tests
100 phosphate HR tests
300 phosphate HR tests
100 cyanuric acid tests
300 cyanuric acid tests
100 chromium (VI) HR tests
300 chromium (VI) HR tests
100 nickel HR tests
ACCESSORIES
11. ACCESSORIES
11.1. REAGENT SETS
Code
HI93700-01
HI93700-03
HI93701-01
HI93701-03
HI93701-F
HI93701-T
HI93702-01
HI93702-03
HI93705-01
HI93705-03
HI93708-01
HI93708-03
HI93710-01
HI93710-03
HI93711-01
HI93711-03
HI93713-01
HI93713-03
HI93715-01
HI93715-03
HI93717-01
HI93717-03
HI93722-01
HI93722-03
HI93723-01
HI93723-03
HI93726-01
95
ACCESSORIES
96
Code
HI93726-03
HI93728-01
HI93728-03
HI93730-01
HI93730-03
HI93732-01
HI93732-03
HI93731-01
HI93731-03
HI93733-01
HI93733-03
HI93737-01
HI93737-03
HI93740-01
HI93740-03
HI93749-01
HI93749-03
HI95747-01
HI95747-03
Description
300 nickel HR tests
100 nitrate tests
300 nitrate tests
100 molybdenum tests
300 molybdenum tests
100 dissolved oxygen tests
300 dissolved oxygen tests
100 zinc tests
300 zinc tests
100 ammonia HR tests
300 ammonia HR tests
50 silver tests
150 silver tests
50 nickel LR tests
150 nickel LR tests
100 chromium (VI) LR tests
300 chromium (VI) LR tests
100 copper LR tests
300 copper LR tests
Description
Triple ceramic, double junction, low temperature glass, refillable pH
electrode with conical tip and temperature sensor
Triple ceramic, double junction, high temperature glass, refillable pH
electrode with temperature sensor
Glass body, double junction, refillable pH/temperature electrode
Glass body, double junction, refillable pH/temperature electrode with
enhanced diagnostics
Plastic body, double junction, gel filled, non refillable pH/temperature
electrode
Plastic body, double junction, gel filled, non refillable pH/temperature
electrode with enhanced diagnostics
Glass body, double junction with temperature sensor for wine analysis
Double junction, open reference, non refillable, electrolyte viscolene, PVDF
body with conical tip, pH/temperature electrode
Double junction, open reference, non refillable, electrolyte viscolene, glass
body with conical tip, pH/temperature electrode
Double junction, open reference, non refillable, electrolyte viscolene, PVDF
body with conical tip, pH/temperature electrode
ACCESSORIES
11.2 pH ELECTRODES
Code
HI10530
HI10430
HI11310
HI11311
HI12300
HI12301
HI10480
FC2320
FC2100
FC2020
Note: The enhanced diagnostics information are not displayed by meter.
97
ACCESSORIES
98
11.3 pH SOLUTIONS
Code
Description
BUFFER SOLUTIONS
HI70004P
pH 4.01 Buffer Sachets, 20 mL (25 pcs.)
HI70007P
pH 7.01 Buffer Sachets, 20 mL (25 pcs.)
HI70010P
pH 10.01 Buffer Sachets, 20 mL (25 pcs.)
HI7001L
pH 1.68 Buffer Solution, 500 mL
HI7004L
pH 4.01 Buffer Solution, 500 mL
HI7006L
pH 6.86 Buffer Solution, 500 mL
HI7007L
pH 7.01 Buffer Solution, 500 mL
HI7009L
pH 9.18 Buffer Solution, 500 mL
HI7010L
pH 10.01 Buffer Solution, 500 mL
HI8004L
pH 4.01 Buffer Solution in FDA approved bottle, 500 mL
HI8006L
pH 6.86 Buffer Solution in FDA approved bottle, 500 mL
HI8007L
pH 7.01 Buffer Solution in FDA approved bottle, 500 mL
HI8009L
pH 9.18 Buffer Solution in FDA approved bottle, 500 mL
HI8010L
pH 10.01 Buffer Solution in FDA approved bottle, 500 mL
ELECTRODE STORAGE SOLUTIONS
HI70300L
Storage Solution, 500 mL
HI80300L
Storage Solution in FDA approved bottle, 500 mL
ELECTRODE CLEANING SOLUTIONS
HI70000P
Electrode Rinse Sachets, 20 mL (25 pcs.)
HI7061L
General Cleaning Solution, 500 mL
HI7073L
Protein Cleaning Solution, 500 mL
HI7074L
Inorganic Cleaning Solution, 500 mL
HI7077L
Oil & Fat Cleaning Solution, 500 mL
HI8061L
General Cleaning Solution in FDA approved bottle, 500 mL
HI8073L
Protein Cleaning Solution in FDA approved bottle, 500 mL
ELECTRODE CLEANING SOLUTIONS
HI8077L
Oil & Fat Cleaning Solution in FDA approved bottle, 500 mL
HI7082
3.5M KCl Electrolyte, 4x30 mL, for double junction electrodes
HI8082
3.5M KCl Electrolyte in FDA approved bottle, 4x30 mL, for double junction
electrodes.
ACCESSORIES
11.4. OTHER ACCESSORIES
Code
Description
HI72083300
carrying case
HI731318
cloth for wiping cuvettes (4 pcs.)
HI731331
glass cuvettes (4 pcs.)
HI731335N
cap for cuvette (4 pcs.)
HI731340
200 µL automatic pipette
HI731341
1000 µL automatic pipette
HI731342
2000 µL automatic pipette
HI740034P
cap for 100 mL beaker (10 pcs.)
HI740036P
100 mL plastic beaker (10 pcs.)
HI740038
60 mL glass bottle and stopper
HI740142P
1 mL graduated syringe (10 pcs)
HI740143
1 mL graduated syringe (6 pcs.)
HI740144
pipette tip (6 pcs.)
HI740157P
plastic refilling pipette (20 pcs.)
HI740220
25 mL graduated glass vial (2 pcs.)
HI740223
170 mL plastic beaker
HI740224
170 mL plastic beaker (12 pcs.)
HI740225
60 mL graduated syringe
HI740226
5 mL graduated syringe
HI740227
filter assembly
HI740228
filter discs (25 pcs.)
HI 740229
100 mL graduated cylinder
DEMI-02
demineralizer
99
Code
HI75110/220E
HI75110/220U
HI76404A
HI83306-11
HI83300-100
HI920015
HI93703-50
HI93703-55
100
Description
USB power adapter, European plug
USB power adapter, USA plug
electrode holder
CAL Check cuvette kit for HI83306
Sample preparation kit consisting of activated carbon for 50 tests, demineralizer bottle for 10 L of water, 100 mL graduated beaker with
cap, 170 mL graduated beaker with cap, 3 mL pipette, 60 mL syringe,
5 mL syringe, graduated cylinder, spoon, funnel, filter paper (25 pcs.).
USB to micro USB cable connector
cuvette cleaning solution (230 mL)
activated carbon (50 pcs.)
ABBREVIATIONS
12. ABBREVIATIONS
EPA:
US Environmental Protection Agency
°C:
degree Celsius
°F:
degree Fahrenheit
µg/L: micrograms per liter (ppb)
mg/L: milligrams per liter (ppm)
g/L:
grams per liter (ppt)
mL:
milliliter
GLP
good laboratory practice
UHR
ultra high range
ULR
ultra low range
HR:
high range
MR:
medium range
LR:
low range
PAN:
1-(2-pyridylazo)-2-naphtol
TPTZ: 2,4,6-tri-(2-pyridyl)-1,3,5-triazine
101
CERTIFICATION
All Hanna Instruments conform to the CE European Directives.
RoHS
compliant
Disposal of Electrical & Electronic Equipment. The product should not be treated as household
waste. Instead hand it over to the appropriate collection point for the recycling of electrical and
electronic equipment which will conserve natural resources.
Disposal of waste batteries. This product contains batteries, do not dispose of them with other
household waste. Hand them over to the appropriate collection point for recycling.
Ensuring proper product and battery disposal prevents potential negative consequences for the
environment and human health. For more information, contact your city, your local household
waste disposal service, the place of purchase or go to www.hannainst.com.
MAN83306 05/19
102
Recommendations
for Users
Warranty
Before using this product, make sure it is entirely suitable for your
specific application and for the environment in which it is used.
Any variation introduced by the user to the supplied equipment
may degrade the meters’ performance. For yours and the meter’s
safety do not use or store the meter in hazardous environments.
The HI83306 is warranted for two years against defects in workmanship and materials
when used for their intended purpose and maintained according to instructions.
Damage due to accidents, misuse, tampering or lack of prescribed maintenance is
not covered.
If service is required, contact your local Hanna Instruments Office. If under warranty,
report the model number, date of purchase, serial number and the nature of the
problem. If the repair is not covered by the warranty, you will be notified of the
charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain
a Returned Goods Authorization (RGA) number from the Technical Service department
and then send it with shipping costs prepaid. When shipping any instrument, make
sure it is properly packed for complete protection.
Hanna Instruments reserves the right to modify the design, construction or appearance of its products
without advance notice.
World
Headquarters
Hanna Instruments Inc.
Highland Industrial Park
584 Park East Drive
Woonsocket, RI 02895 USA
www.hannainst.com
MAN83306
Printed in ROMANIA
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