Hanna Instruments | HI96812 | Owner Manual | Hanna Instruments HI96812 Owner Manual

Hanna Instruments HI96812 Owner Manual
Refractometer for Sucrose
in Wine and Grape Products
Measurements
INSTRUCTION MANUAL
HI96812
Dear
Customer,
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using
this instrument.
This manual will provide you with the necessary information
for correct use of this instrument, as well as a precise idea
of its versatility.
If you need additional technical information, do not hesitate
to e‑mail us at tech@hannainst.com or view our worldwide
contact list at www.hannainst.com.
All rights are reserved. Reproduction in whole or in part is prohibited
without the written consent of the copyright owner, Hanna Instruments
Inc., Woonsocket, Rhode Island, 02895, USA.
TABLE OF CONTENTS
Preliminary Examination........................................................ 4
General Description................................................................ 5
Specifications........................................................................ 6
Principle of Operation............................................................ 7
Functional Description............................................................ 8
Display Elements................................................................... 9
Measurement Guidelines........................................................ 10
Calibration Procedure............................................................. 11
Measurement Procedure......................................................... 12
Making a Standard % Brix Solution....................................... 14
To Change Temperature Unit.................................................. 15
Battery Replacement.............................................................. 16
Error Messages...................................................................... 17
Accessories............................................................................ 18
3
PRELIMINARY EXAMINATION
4
Remove the instrument from the packing materials and examine carefully
to ensure no damage has occurred during shipping. If any damage has
occurred, contact your local Hanna Instruments Office.
Each HI96812 instrument is supplied with:
• 9 V battery
• Instruction manual
• Plastic pipette
• Quality Certificate
Note: Save all packing material until you are sure that the instrument
functions correctly. A defective instrument must be returned in its
original packing.
GENERAL DESCRIPTION
The HI96812 Digital Wine Refractometer is rugged portable, water resistant
devices that benefit from Hanna Instruments’ years of experience as a
manufacturer of analytical instruments. Hanna Instruments offers five related
wine refractometers to fill the differing requirements found in the industry.
All are equally valid ways to measure the sugar content of grape or must
samples in the field or winery.
The HI96812 is optical instruments that are based on the measurement of
the refractive index of a solution. The measurement of refractive index is
simple and quick and provides the vintner a standard accepted method for
sugar content analysis. Samples are measured after a simple user calibration
with deionized or distilled water. Within seconds the instrument measures
the refractive index of the grape. These digital refractometers eliminate the
uncertainty associated with mechanical refractometers and are easily por‑
table for measurements in the field. Temperature (in °C or °F) is displayed
simultaneously with the measurement on the large dual level display along
with icons for Low Power and other helpful message codes.
Key features include:
• Dual-level LCD
• Automatic Temperature Compensation (ATC)
• Easy setup and storage
• Battery operation with Low Power indicator (BEPS)
• Automatically turns off after 3 minutes of non-use
• Single-point calibration with distilled or deionized water
• Waterproof models offers IP65 waterproof protection
• Quick, precise results readings are displayed in approximately 1.5 seconds
• Small sample size as small as 2 metric drops.
5
SPECIFICATIONS
6
Range
0.0 to 28.0 °Baumé/ 0.0 to 80.0 °C
(32.0 to 176.0 °F)
Resolution
0.1 °Baumé/ 0.1 °C (0.1 °F)
Accuracy
± 0.1 °Baumé /
± 0.3 °C (±0.5 °F)
Temperature Compensation
Automatic between 10.0 and 40.0 °C
(50.0 - 104.0 °F)
Measurement Time
Approximately 1.5 seconds
Minimum Sample Volume
100 µL (cover prism totally)
Light Source
Yellow LED
Sample Cell
Stainless Steel ring and flint glass prism
Case Material
ABS
Enclosure Rating
IP65
Battery Type / Life
9V / 5000 readings
Auto-Off
After 3 minutes of non-use
Dimensions
Mass
192 x 102 x 69 mm (7.6 x 4.1 x 2.7”)
350 g (12.3 oz)
PRINCIPLE OF OPERATION
The °Baumé determination is made by measuring the refractive index of a
solution. Refractive Index is an optical characteristic of a substance and the
number of dissolved particles in it. Refractive Index is defined as the ratio of
the speed of light in empty space to the speed of light in the substance. A
result of this property is that light will “bend”, or change direction, when it
travels through a substance of different refractive index. This is called refraction.
When passing from a material with a higher to lower refractive index, there
is a critical angle at which an incoming beam of light can no longer refract,
but will instead be reflected off the interface. The critical angle can be used
to easily calculate the refractive index according to the equation:
sin (θcritical) = n2 / n1
Where n2 is the refractive index of the lower-density medium; n1 is the
refractive index of the higher-density medium.
In the HI96812, light from an LED passes through a prism in contact with
the sample. An image sensor determines the critical angle at which the light
is no longer refracted through the sample.
The °Baumé scale is based on density and was originally designed to mea‑
sure the mass of sodium chloride in water. °Baumé is used in wine making
to measure the sugar in must. The HI96812 converts the %Brix reading
to °Baumé based on the table found in the Official Methods of Analysis
of AOAC International, 18th Edition. 1 °Baumé is approximately equal to
1.8 %Brix, and 1 °Baumé is roughly equivalent to 1 % alcohol when the
wine is fully fermented.
7
FUNCTIONAL DESCRIPTION
TOP VIEW
1. Liquid Crystal Display (LCD)
2. Secondary Display
3. Primary Display
4. READ Key (User Measurement)
5. ZERO Key (User Calibration)
6. ON/OFF
7. Stainless Steel Sample Well and Prism
BOTTOM
8. Battery Cover
9. Battery Compartment
8
DISPLAY ELEMENTS
1.
2.
3.
4.
5.
6.
7.
Battery (blinks when low battery condition detected)
Primary Display (displays measurement and error messages)
Measurement in Progress Tag
SETUP: Factory Calibration Tag
CAL: Calibration Tag
Measurement Units
Automatic Temperature Compensation
(blinks when temperature exceeds 10.0-40.0 °C /50.0-104.0 °F range)
8. Temperature Units
9. Secondary Display (displays temperature measurements; when
blinking, temperature has exceeded operation range: 0.0-80.0 °C /
32.0-176.0 °F)
9
MEASUREMENT GUIDELINES
10
• Handle instrument carefully. Do not drop.
• Do not immerse instrument under water.
• Do not spray water to any part of instrument except the “sample well”
located over the prism.
• The instrument is intended to measure Wine and Grape products. Do not
expose instrument or prism to solvents that will damage it. This includes
most organic solvents and extremely hot or cold solutions.
• Particulate matter in a sample may scratch the prism. Absorb sample
with a soft tissue and rinse sample well with deionized or distilled water
between samples.
• Use plastic pipettes to transfer all solutions. Do not use metallic tools
such as needles, spoons or tweezers as these will scratch the prism.
• To reduce the effects of evaporation or absorption of water when taking
readings over a period of time, the prism and sample well can be covered
with plastic wrap.
CALIBRATION PROCEDURE
Calibration should be performed daily, before measurements are made,
when the battery has been replaced, between a long series of measure‑
ments, or if environmental changes have occurred since the last calibration.
1. Press the ON/OFF key, then release. Two instrument test screens will
be displayed briefly; all LCD segments followed by the percentage of
remaining battery life. When LCD displays dashes, the instrument is ready.
2. Using a plastic pipette, fill the sample well with distilled or deionized
water. Make sure the prism is completely covered.
Note: If the ZERO sample is subject to intense light such as sunlight
or another strong source, cover the sample well with your hand or
other shade during the calibration.
3. Press the ZERO key. If no error messages appear, your unit is calibrated.
(For a description of ERROR MESSAGES see page 17).
Note: The 0.0 screen will remain until a sample is measured or the
power is turned off.
11
CALIBRATION PROCEDURE
4. Gently absorb the ZERO water standard with a soft tissue. Use care not
to scratch the prism surface. Dry the surface completely. The instrument
is ready for sample measurement.
Note: If the instrument is turned off the calibration will not be lost.
MEASUREMENT PROCEDURE
Verify the instrument has been calibrated before taking measurements.
1. Wipe off prism surface located at the bottom of the sample well. Make
sure the prism and sample well are completely dry.
12
2. Using a plastic pipette, drip sample onto the prism surface. Fill the well
completely.
Note: If the temperature of the sample differs significantly from the
temperature of the instrument, wait approximately 1 minute to allow
thermal equilibration.
Note: The last measurement value will be displayed until the next
sample is measured or the instrument is turned off. Temperature will be
continuously updated.
The ATC tag blinks and automatic temperature compensation
is disabled if the temperature exceeds the 10.0-40.0 °C/
50.0-104.0 °F range.
MEASUREMENT PROCEDURE
3. Press the READ key. The result is displayed in units of °Baumé.
4. Remove sample from the sample well by absorbing with a soft tissue.
5. Using a plastic pipette, rinse prism and sample well with distilled or
deionized water. Wipe dry. The instrument is ready for the next sample.
13
MAKING A STANDARD % BRIX SOLUTION
14
To make a Brix Solution, follow the procedure below:
• Place container (such as a glass vial or dropper bottle that has a cover)
on an analytical balance.
• Tare the balance.
• To make an X% BRIX solution weigh out X grams of high purity Sucrose
(CAS #: 57-50-1) directly into the container.
• Add distilled or deionized water to the container so the total weight of
the solution is 100 g.
Note: Solutions above 60 % Brix need to be vigorously stirred or shaken
and heated in a water bath. Remove solution from bath when sucrose
has dissolved. The total quantity can be scaled proportionally for smaller
containers but accuracy may be sacrificed.
Example with 25% Brix:
%Brix
g Sucrose
25
25.000
g Water
75.000
g Total
100.000
15 seconds
2. While continuing to hold the ON/OFF key, press the ZERO key. The
temperature unit will change from °C to °F or vice versa.
TO CHANGE TEMPERATURE UNIT
To change the temperature measurement unit from Celsius to Fahrenheit
(or vice versa), follow this procedure.
1. Press and hold the ON/OFF key continuously for approximately
15 seconds. The LCD will display the “all segment” screen followed by
a screen with the model number on the primary display and the version
number on the secondary display. Continue pressing the ON/OFF key.
°C or °F
15
BATTERY REPLACEMENT
To replace the instrument’s battery, follow these steps:
• Make sure the instrument is off.
• Turn instrument upside down and remove the battery cover by turning
it counterclockwise.
• Extract the battery from its location.
• Replace with a new 9V battery making sure to observe polarity.
• Insert the back battery cover and fasten it by turning clockwise to
engage.
16
Description
“Err“
General failure. Cycle power to instrument.
If error persists, contact your local Hanna
Instruments Office.
“LO“ primary
display
Sample exceeds minimum measurement
range.
“HI“ primary
display
Sample exceeds maximum measurement
range.
“LO“ primary
display Cal
segment ON
Wrong solution used to zero instrument.
Use deionized or distilled water. Press
ZERO.
“HI“ primary
display Cal
segment ON
Wrong solution used to zero instrument.
Use deionized or distilled water. Press
ZERO.
“t LO“ primary
display Cal
segment ON
Temperature exceeds ATC low limit
(10.0 °C) during calibration.
“t HI“ primary
display Cal
segment ON
Temperature exceeds ATC high limit
(40.0 °C) during calibration.
“Air“
Prism surface insufficiently covered.
“ELt“
Too much external light for measurement.
Cover sample well with hand.
“nLt“
LED light is not detected.
Contact your local Hanna Instruments
Office.
Battery segment
blinking
<5% of battery life is remaining.
Temperature values
are blinking
“0.0 °C“ or “80.0 °C“
ERROR MESSAGES
Error Code
Temperature measurement
out of range (0.0 to 80.0 °C).
ATC segment
blinking
Outside temperature
compensation range
(10.0 to 40.0 °C).
SETUP segment
blinking
Factory calibration lost.
Contact your local Hanna Instruments
Office.
17
ACCESSORIES
Code
Description
HI4020-11
50% Brix Standard, 10 mL
HI740157P
Plastic refilling pipette (20 pcs.)
HI740029P
9V Battery (10 pcs.)
MAN96812 04/17
18
Warranty
HI96812 is warranted for two years against defects in
workmanship and materials when used for their intended
purpose and maintained according to instructions.
This warranty is limited to repair or replacement free of
charge.
Damage due to accidents, misuse, tampering or lack of
prescribed maintenance is not covered.
If service is required, contact your local Hanna Instruments
Office. If under warranty, report the model number, date
of purchase, serial number and the nature of the problem.
If the repair is not covered by the warranty, you will be
notified of the charges incurred. If the instrument is to be
returned to Hanna Instruments, first obtain a Returned Goods
Authorization number from the Technical Service department
and then send it with shipping costs prepaid. When ship‑
ping any instrument, make sure it is properly packed for
complete protection.
To validate your warranty, fill out and return the enclosed
warranty card within 14 days from the date of purchase.
Hanna Instruments reserves the right to modify the design, construction or
appearance of its products without advance notice.
World
Headquarters
Hanna Instruments Inc.
Highland Industrial Park
584 Park East Drive
Woonsocket, RI 02895 USA
www.hannainst.com
Local Office
Hanna Instruments USA
270 George Washington Highway
Smithfield, RI 02917
Phone: 800.426.6287
Fax: 401.765.7575
e-mail: tech@hannainst.com
MAN96812
Printed in ROMANIA
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