Leica Microsystems | LED8 | User manual | Leica Microsystems LED8 Light Microscope Accessories User manual

Leica Microsystems LED8 Light Microscope Accessories User manual
From Eye to Insight
Leica
Leica
Leica
Leica
LED1
LED3
LED5
LED8
External light sources for Leica microscopes
Instructions for use
Leica Microsystems CMS GmbH. Instructions for use, 11934173, Revision 1.0, 2019.05.01
Document Number 57-10022 Revision A
1
Copyright
Copyright
All rights to this documentation are held by
Leica Microsystems CMS GmbH. Reproduction
of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method
(including electronic systems) is not allowed
without express written permission from Leica
Microsystems CMS GmbH.
The instructions contained in the following documentation reflect state-of-the-art technology and
knowledge standards. We have compiled the
texts and illustrations as accurately as possible.
Nevertheless, no liability of any kind shall be accepted for the accuracy of this manual's contents. Still, we are always grateful for comments
and suggestions regarding potential errors within
this documentation.
The information included in this manual may be
changed without prior notice.
Revision 1.0, edited 2019.05.01 by:
Leica Microsystems CMS GmbH
Ernst-Leitz-Strasse 17–37
D-35578 Wetzlar (Germany)
http://www.leica-microsystems.com
Responsible for contents:
Marketing CMS
2
As the official manufacturer of Leica branded
light engine products, Lumencor is the responsible owner of this instructions for use 57-10022,
Revision A.
Leica Microsystems CMS GmbH is the distributor
of the Lumencor illumination products detailed
herein, 11934173, Revision 1.0 2019.05.01 is for
Leica internal tracking purposes only.
Contents
Contents
1.
Important Notes about this Manual..............4
2.
Function of the Compact Light Source.........6
3.
3.1
3.1.1
3.1.2
3.1.3
3.1.4
3.2
3.3
Safety Notes......................................................7
Regulatory safety notes..................................7
Introduction LED1.............................................8
Introduction LED3.............................................8
Introduction LED5/LED8...................................8
Precautions and Warnings ............................9
Electrical safety..............................................12
Disposal...........................................................15
4.
Transport, Storage, Unpacking...................16
5.
Notes on Warranty.........................................17
6.
6.1
6.2
6.3
6.4
6.5
6.6
Overview of the Instrument..........................18
Intended use...................................................18
Identification of the instrument....................18
Special features of the instrument..............18
Overview Light Sources................................19
Connecting LED1............................................23
Connectable light guides (LED3-8)..............23
7.
7.1
7.2
7.3
7.4
7.5
Instrument Setup............................................24
Installation.......................................................24
Connecting the LED1......................................24
LED3/5/8 – connecting the light guide........24
Connecting the Leica POD (optional)..........26
Connecting the power cable........................26
8.
8.1
8.2
8.3
8.3.1
Operation.........................................................27
Operation LED1...............................................27
Operation LED3...............................................27
Operation LED5/8............................................29
Start Up............................................................30
9.
9.1
9.2
Service.............................................................31
Maintenance and cleaning...........................31
Repairs.............................................................31
10.
Troubleshooting.............................................32
11.
EC Declaration of Conformity......................33
3
1. Important Notes about this Manual
1. Important Notes about this Manual
Attention!
This User Manual is an essential component
of the instrument and must be read carefully
before assembly, commissioning or use.
This User Manual contains important instructions and information for the operational safety
and maintenance of the instrument and accessories. Therefore, it must be kept in a safe place.
Text symbols, pictograms and their meanings:
(1.2)
Numbers in parentheses refer to illustrations; for
example, (1.2) refers to Fig. 1, item 2.
→ page 20
Numbers with a pointer arrow (for example →
page 20), point to a certain page of this manual.
WARNING
CAUTION
WARNING indicates a potentially hazardous
situation with a medium degree of risk that, if
not avoided, could result in death or serious
injury.
CAUTION indicates a potentially hazardous situation with a low degree of risk that, if not avoided,
may result in minor or moderate injury.
Attention!
In this manual, additional safety notes are indicated with the triangle symbol shown here,
and have a gray background.
!
4
Attention! The instrument and its accessories
can be damaged when operated incorrectly.
1. Important Notes about this Manual
Warning of hazardous electrical voltage! Danger
of electric shock!
Warning of hot surface.
Warning: Possibly hazardous optical radiation
emitted from this product. Do not look at operating lamp. Eye injury may result.
Warning: UV emitted from this product. Avoid eye
and skin exposure to unshielded product.
UV
Warning: IR emitted from this product. Do not
look at operating lamp.
IR
Warning of electromagnetic field
Instructions for disposing of the instrument,
its accessories and consumables.
Earth (Ground) terminal
Explanatory note.
*
MM/YYYY
Item not contained in all configurations.
Manufacturer's address with manufacturing date
China RoHS 50 years EFUP
(Environmentally friendly use period)
5
2. Function of the Compact Light Source
2. Function of the Compact Light Source
The Leica LED1, LED3, LED5 and LED8 compact
light sources, with which this instructions for use
are associated, are intended for biological routine and research work as well as examinations
in materials science or mineralogy.
Reasonably foreseeable misuse
The following are prohibited:
• Using the light source for any purpose not in
accordance with the Declaration of Conformity
(e.g. use as a medical product in accordance
with EU Directive 93/42/EEC).
• Cleaning the light source in a way other than
specified in the manual.
• Modifying the built-in safety circuits in the instrument.
• Allowing unauthorized personnel to open the
instrument.
• Using cables that Leica has not provided or
permitted.
• Using combinations with non-Leica components that go beyond the scope of the User
Manual.
• Operating the light source while it is not connected to the stand.
6
Attention!
The manufacturer assumes no liability for
damage caused by, or any risks arising from,
using the light source for other purposes than
those for which they are intended or not using them within the specifications of Leica
Microsystems CMS GmbH.
In such cases, the Declaration of Conformity
shall be invalid.
Attention!
This instrument is not intended for use in a
patient environment as defined by DIN VDE
0100-710. It is also not intended to be combined with medical devices as defined by
EN 60601-1. If a microscope is electrically
connected to a medical instrument in accordance with EN 60601-1, the requirements defined in EN 60601-1-1 shall apply.
3. Safety Notes
3. Safety Notes
3.1
Regulatory safety notes
Leica Microsystems utilizes regulatory model
names for all certified and CE marked products.
The regulatory model names are traceable to
all regulatory documentation, third party reports
and certifications.
“Regulatory Model: LED1/LED3/LED5/LED8 is
used as a representative model for all certified
and CE marked LED1/LED3/LED5/LED8 Products.
Emissions
This equipment has been tested and found to
comply with the limits of EMC directive 2014/30/
EU. These limits are designed to provide reasonable protection against harmful interference
when the equipment is operated in a commercial
environment. This equipment generates, uses,
and can radiate radio frequency energy and, if
not installed and used in accordance with the
instruction manual, may cause harmful interference to radio communications.
It also meets the requirements of EN 61326-1,
Electrical equipment for measurement, control
and laboratory use – EMC requirements.
Photobiological Safety
Safety Certifications
TÜV SÜD America, CB Certification
(IEC 61010-1:2010)
TÜV SÜD America, NRTLus Certification
(UL 61010-1:2012)
TÜV SÜD America, cNRTL Certification
(CAN/CSA-C22.2 No. 61010-1:2012)
TÜV SÜD America, EN Certification
(EN 61010-1:2010)
CE Marking
Low Voltage Directive (2014/35/EU)
EMC Directive (2014/30/EU)
RoHS Directive (2011/65/EU) plus (2015/863/EU)
REACH Regulation (EC) No. (1907/2006/EC)
In accordance with the REGULATION (EU) No.
1194/2012 OF THE COMMISSION for the implementation of the Directive 2009/125/EC of the
European Parliament and Council with regard to
the requirements for the environmentally friendly
design of lamps with highly focused light, LED
lamps and associated instruments, the products
described here, Leica LED1, LED3, LED5 and
LED8, are special products because they are not
suitable for general lighting purposes.
The compact light sources have also been tested
in accordance with EN 62471 / IEC 62471 Photobiological safety of lamps and lamp systems and
is classified in risk group 3 (high risk).
7
3. Safety Notes
3.1.1 Introduction LED1
3.1.3 Introduction LED5/LED8
The LED1 light source is a compact solid-state
light source for fluorescence microscopy and
other bioanalytical applications. The light source
incorporates efficient thermal management, requiring no fan and providing quiet and vibrationfree operation. This enables direct coupling to
the fluorescence epi-illumination port of a microscope.
The LED light source consists of 4 (LED5) and 8
(LED8) individually addressable solid-state light
sources with integrated electronic control systems. The constituent light sources may be LEDs
or luminescent light pipes.
The outputs of the constituent light sources are
refined by bandpass filters and merged into a
common optical train directed to the light output
port on the front panel. The light output port has
a built-in adapter for connection to a liquid light
guide (LLG). An onboard photodiode continuously
monitors the light output and generates reference signals that can be applied to the constituent sources in a feedback loop to maintain constant light output over time.
The LED5/8 light sources are controlled by onboard micro-processors that connect to LAS X
imaging software via a RS-232 (PC) serial interface. The user can enable or disable each
source independently by serial commands, as
well as change the intensity of each source independently. Alternatively, the light sources may
be turned on and off by TTL inputs from a trigger
device such as a camera or a real-time controller. The only manual control is the master power
switch on the front panel. Optimal internal operating temperature is maintained by negative
pressure air cooling with the air intake at the
front of the light engine and the exhaust fan at
the the rear.
The light source provides four distinct color
bands of light output that are controlled using
either manual control knobs on the side panel
or the light source control POD accessory. The
COLOR knob located on the side panel is used for
on/off control, selection of color channels and for
enabling external tablet control. The INTENSITY
knob provides four different levels of output attenuation between 100% and 10% of maximum.
3.1.2 Introduction LED3
The LED3 light sources are designed for laboratory use by bioanalytical researchers and/
or developers of life science instrumentation.
The light sources generate white light output by
combining the outputs of multiple solid state light
sources. The light output on/off status is manually controlled via a rocker switch located on the
front panel or a foot pedal toggle switch accessory that plugs into the 3.5 mm connector on the
rear panel.
8
3. Safety Notes
3.1.4 Precautions and Warnings
A few simple practices will ensure trouble-free
operation for the life of the light engine.
Safety Instructions:
Please read and follow all safety instructions
provided BEFORE using the light source. Failure
to comply with the safety instructions may result
in fire, electrical shock, or personal injury and
may damage or impair protection provided by
equipment. Please save all safety instructions.
In order to maintain this condition and to ensure
safe operation, the user must follow the instructions and warnings contained in this User Manual.
Warning: DO NOT look into the output of the
light engine. The brightness of this light source
is higher than most commercial lighting fixtures
and is intended to couple directly into a microscope or other bioanalytjcal instrument.
Warning: DO NOT turn on the light without the
output end of the light guide safely directed
into an enclosed optical path. DO NOT point the
light output directly onto any flammable or burnsusceptible material. This includes all animal or
vegetable tissues, plastics, fabrics, paper and
liquids
RISK GROUP 3
Applicable for LED1/3/5/8
RISK GROUP 3
WARNING Possibly hazardous optical
radiation emitted from this product.
Do not look at operating lamp.
Eye injury may result.
Safety Definitions
Groupe de risque 3
AVERTISSEMENT Ce produit émet un
rayonnement optique potentiellement
dangereux. Ne regardez pas la lampe
lorsqu‘elle est allumée. Des lésions
oculaire peuvent en résulter.
Warning: Statements identify conditions or practices that could result in personal injury
Caution: Statements identify conditions or practices that could result in damage to your equipment.
Safety Items
Warning: ONLY use the power supply provided
by Leica. It is imperative that the DC power supply has output over-current protection, as the
power input of the light sources are not fused.
The DC power supply must have the AC power
cord connected to a receptacle with a protective
safety (earth) ground terminal
Applicable for LED3/5/8
RISK GROUP 3
WARNING IR emitted from this product.
Do not look at operating lamp. Avoid eye
exposure. Use appropriate shielding or
eye protection.
IR
Groupe de risque 3
AVERTISSEMENT Ce produit émet un
rayonnement infrarouge. Ne regardez pas
la lampe lorsqu‘elle est allumée.
Évitez tout exposition aux yeux.
Utilisez un écran ou dispositif de
protection oculaire approprié.
9
3. Safety Notes
Applicable for LED1/3/5/8
RISK GROUP 3
WARNING UV emitted from this product.
Avoid eye and skin exposure to
unshielded product
UV
Groupe de risque 3
AVERTISSEMENT Ce produit émet un
rayonnement ultraviolet. Évitez d‘exposer
les yeux et la peau à un produit sans
protection.
The Leica LED1, LED3, LED5 and LED8 compact light sources generate high-energy light
with invisible UV components and has been
classified in risk group 3 (high risk) in accordance with EN62471-1 while the light guide
is not connected to the microscope/system.
There is a general risk of being dazzled or
blinded by light! Never look directly into the
light guide output of the instrument or into
the output of the light guide connected to the
instrument. Always ensure that the output of
the connected light guide is securely connected to the system to be illuminated before
switching on the compact light source. Furthermore, before switching on the compact
light source, dim the light output to prevent
damage to the connected system. During operation, the light guide must not be removed
from the microscope/system. While connected to the microscope/system, the risk group 1
(low risk) has been classified. Please see the
instructions on this topic in the microscope
instructions for use.
10
Warning: Possibly hazardous optical radiation
emitted from this product. Do not look at operating lamp. Eye injury may result.
Warning: Infrared (IR) emitted from this product.
Do not look at operating lamp.
Warning: UV emitted from this product. Avoid
eye and skin exposure to unshielded product.
Caution: DO NOT open the unit. There are no
serviceable parts inside and opening the light
engine enclosure will void the manufacturer’s
warranty.
Caution: DO NOT set liquids on the light engine.
Spilled liquids may damage your light engine.
Caution: DO NOT drop the light engine. It contains glass optical components that could be
damaged or misaligned by the shock produced
by a drop onto a hard surface.
DISCLAIMER: Leica shall not be liable for injury to
the user or damage to the product resulting from
the light source being used in a way for which it
was not intended and in complete disregard for
all posted safety precautions and warnings.
3. Safety Notes
Attention!
The instruments and accessories described
in this Instructions for use have been tested
for safety and potential hazards.
The responsible Leica representative or the
main plant in Wetzlar must be consulted
whenever the instrument is altered, modified
or used in conjunction with non-Leica components that are outside of the scope of this
manual!
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims!
Attention!
If any safety defects or malfunctions of the
compact light sources are identified, the
instrument must be immediately taken out
of operation, disconnected from the power
system at all poles and secured against further use. For repair, the compact light source
must be sent to the vendor or an authorized
representative of the vendor.
11
3. Safety Notes
3.2
Electrical safety
General specifications
For indoor use only.
Frequency:
50/60 Hz
Ambient temperature:
0 to 35 °C
Relative humidity:10 to 80 %
non-condensing
Overvoltage category:
II
Pollution degree:
2
LED1
Supply voltage:
9 V DC, 4.45 A
Power input:
40 W max.
Fuses:Integrated in external
power supply unit, not
replaceable
LED3
Supply voltage:
24 V DC, 5.00 A
Power input:
120 W max.
Fuses:Integrated in external
power supply unit, not
replaceable
Specifications of the external power supply
One of the power supply units listed below
(LPS-certified) must be used:
Mean Well GST120A24-P1M
Specifications of the external power supply
Shared technical data:
Input: 100-240 V AC
50/60 Hz
Output:
24 V DC
5.00 A max.
120 W max.
One of the power supply units listed below
(LPS-certified) must be used:
LED5
Mean Well GST40A09-P1J
Shared technical data:
Input: 100-240 V AC
50/60 Hz
Output:
9 V DC
4.45 A max.
40 W max.
Supply voltage:
24 V DC, 9.20 A
Power input:
220 W max.
Fuses:Integrated in external
power supply unit, not
replaceable
Specifications of the external power supply
One of the power supply units listed below
(LPS-certified) must be used:
Mean Well GST220A24-LC
Shared technical data:
Input: 100-240 V AC
50/60 Hz
Output:
24 V DC
9.2 A max.
220 W max.
12
3. Safety Notes
LED8
Supply voltage:
24 V DC, 9.20 A
Power input:
220 W
Fuses:Integrated in external
power supply unit, not
replaceable
WARNING
Only use original power cables or alternative
cables with VDE/HAR codes that minimally
fulfill the following conditions: 3 x 0.75 mm2
and 10 A / 250 V.
Specifications of the external power supply
One of the power supply units listed below
(LPS-certified) must be used:
Mean Well GST220A24-LC
Shared technical data:
Input: 100-240 V AC
50/60 Hz
Output:
24 V DC
9.2 A max.
220 W max.
WARNING
By definition, the main circuit breaker of this
instrument is the connection between the
power cable and instrument port. The user
must ensure unobstructed access to the
main circuit breaker at all times.
CAUTION
Disconnect the power plug before cleaning!
Protect electrical components from moisture.
13
3. Safety Notes
WARNING
The light source must be placed at least
15 cm away from the wall and away from
flammable substances.
Attention!
Do not plug in or unplug data lines and control circuits unless the instrument has been
shut down; otherwise, the instrument may be
damaged.
Attention!
This light source must not be used at altitudes over 2,000 m above sea level.
WARNING
The power plug may only be plugged into an
outlet equipped with a grounding contact.
Do not invalidate the grounding function by
using an extension cord without a ground
wire. Any interruption of the ground wire inside or outside of the instrument, or release
of the protective conductor terminal, can
cause the instrument to become hazardous.
Intentional ground interruption is not permitted!
Attention!
The compact light source are designed for
a voltage range of 100 to 240 VAC, 50/60 Hz.
Within this voltage and frequency range, the
instrument always adapts to the connected
power supply. Operating the instrument with
a power supply voltage outside this range
can destroy the instrument and the connected components!
14
Attention!
The instrument is intended exclusively for
operation in dry rooms. Do not use the instrument in rooms with explosion hazard.
Attention!
Do not use this instrument near sources of
high electromagnetic radiation (for example,
unshielded, intentionally operated ultra-high
frequency sources), because these can disrupt proper operation.
We recommend assessing the electromagnetic environment before operation of this
instrument and then giving corresponding
instructions.
3. Safety Notes
WARNING
Light sources pose a potential irradiation risk
(glare, UV radiation, IR radiation). Therefore,
lamps have to be operated in closed housings and in installed condition.
Never look directly into the beam path (blinding hazard).
3.3
Disposal
Once the product has reached the end of its service life, please contact Leica Service or Sales
about disposal.
Please observe and comply with the national and
federal laws and regulations implementing the
WEEE EC directive, for example.
Note!
Like all electronic devices, the microscope,
its accessory components and consumables
must never be disposed of with general
household waste.
15
4. Transport, Storage, Unpacking
4. Transport, Storage, Unpacking
Attention!
Attention!
Transport and storage at -20 °C to +70 °C and
max. 80 % humidity (non-condensing).
For unpacking, open the box on its top. It is
then possible to lift the light source out of the
box complete with the transport protection
made of foam. After that, the foam corners
and the plastic bag must be removed from the
instrument. After unpacking, the light source
should acclimatize for about 1 hour before
starting up. This is done to prevent damage
by condensation water that can form due to
temperature differences in the interior of the
instrument.
Attention!
The environmental conditions regarding
transport and storage specified in this Instructions for use must be complied with. For
safe transport of the external light source,
the original packaging must be used.
If during unpacking of the light source, damage to the instrument is detected, the vendor
or the shipping company contracted by the
vendor must be notified immediately.
16
5. Notes on Warranty
5. Notes on Warranty
The manufacturer of the instrument warrants
that the instrument is free of material or production defects at the time of delivery. Any defects
that have occurred must be reported immediately, and everything must be done to minimize damage. If such a defect is reported, the vendor of
the instrument is obliged to remedy the defect at
the State Laboratory Services Office's discretion
either by repair or by shipment of an instrument
free of defects. There is no warranty for defects
resulting from natural wear (in particular, in wear
parts) and improper handling.
Attention!
The instrument manufacturer and the vendor of the instrument assume no liability for
damage caused by incorrect operation, negligence, impermissible use, intervention on
the instrument or the use of spare parts and
components that are not approved. In these
cases, all warranty claims are voided.
No maintenance or repair tasks except for those
listed in this instructions for use may be carried
out on the instrument. Repairs may be performed
only by the vendor of the instrument or the vendor's specially authorized representatives.
17
6. Overview of the Instrument
6. Overview of the Instrument
6.1
!
Intended use
Attention!
The LED 3,5, and 8 light sources are used to generate light with a very high intensity and couple
it into the light guide, preferably a liquid light
guide with an active diameter of 3 mm. Any other
use of the instrument is considered noncompliant use.
.
6.2
Identification of the instrument
The nameplate of the instrument is on its rear
side. It includes the necessary information for
identifying the instrument.
In order to maintain this condition and to ensure
safe operation, the user must follow the instructions and warnings contained in this Instructions
for use.
6.3
Special features of the instrument
For LED3/LED5/LED8, the external light source
has an electrical light output interlock. This ensures that the light beam output of the instrument
is closed when the light guide is detached. This
prevents the user from being harmed by the intense light beam generated by the instrument.
Depending on the model of light engine, the light
source can be turned on/off through a variety of
methods. Please confirm the correct light source
operating methods are being used for the instrument:
LED1: The light source can be turned on/off with
the manual light on/off switch on the side panel.
LED3: The light source can be turned on/off with
the manual light on/off switch on the front panel
or in combination with the electronic shutter input on the rear panel.
LED5/8: There is no manual light on/off switch on
the light engine. The light source can be turned
on/off with either the LAS X software or with the
Leica Control POD.
WARNING
The power supply must be set up so that the
power voltage input is not obstructed and the
instrument can quickly be removed from the
power supply if necessary.
18
6. Overview of the Instrument
6.4
Overview Light Sources
Fig. 1 LED1
1 Output adapter
2 On/Off and color switch
3 Intensity switch
4 Light output indicator light
Fig. 2 LED1
1 USB A connection for POD
2 Electronic shutter TTL input
3 Power indicator light
4 Receptacle DC power supply
2
1
3
4
2
3
4
1
Table 1 Output power spectrum LED1
19
6. Overview of the Instrument
Fig. 4 LED3
1 Power indicator light
2 On/Off switch
3 Foot switch
4 Receptacle DC power supply
5 Electronic shutter TTL input
Fig. 3 LED3
1 Light indicator
2 Light switch
3 Output adapter
1
1
2
2
3
3
5
Table 2 Output power spectrum LED3
20
4
6. Overview of the Instrument
Fig. 5 LED5 front
1 Status indicator display
2 Light indicator
3 Interlock
4 TTL connection
5 USB B connection
6 RS-232 connection
7 Receptacle DC power supply
8 On/Off switch
9 Key control
10 Output adpater
11 Manual interlock reset
Fig. 6 LED5 rear
1
8
2
3
9
4
5
10
6
7
11
Table 3 Output power spectrum LED5
21
6. Overview of the Instrument
Fig. 7 LED5 front
1 Status indicator display
2 Light indicator
3 Interlock
4 TTL connection
5 USB B connection
6 RS-232 connection
7 Receptacle DC power supply
8 On/Off switch
9 Key control
10 Adpater
11 Manual interlock reset
Fig. 8 LED5 rear
1
8
2
3
9
4
5
10
6
7
11
Table 4 Output power spectrum LED8
22
6. Overview of the Instrument
6.5
Connecting LED1
The LED1 can be directly installed to the lamp
housing receptacle.
6.6
Connectable light guides (LED3-8)
The light source is compatible with liquid light
guides that are compatible with a "Storz long"
light inlet and whose transmission curve fits the
lamp used. Where applicable, refer to the lamp's
data sheet for its spectral light distribution. The
instrument is optimized for light guides with an
active diameter of 3 mm.
Your Leica vendor will be glad to provide information about suitable light guides.
23
7. Instrument Setup
7. Instrument Setup
7.1
!
Installation
Attention!
Set up the external light source its control and
display elements is readily accessible and visible. The instrument must stand upright safely and
so that it does not skid. The ventilation slots on
the sides and back panel of the instrument must
not be covered. Maintain a free room of at least
150 mm on the rear panel of the instrument. The
cables and light guides to be connected to the
light source must not cause any hazard.
7.2
Connecting the LED1
The LED1 has to be connected to the microscope
before it is turned on.
Be aware that although the human visual system
does not register the wavelengths of light from
the violet color channel, exposure can quickly
produce eye damage. When setting up the light
source, be sure that the output adapter is securely attached to the appropriate illumination
port of the microscope.
Fig. 9 LED1
1 Output adapter
Usually this will be the fluorescence epi-illumination port. Be careful to properly support the light
source until it is securely attached to the instrument.
7.3
!
LED3/5/8 – connecting the light guide
Attention!
The red dust caps MUST BE removed before the
light guide is connected to the light source and
the microscope.
Always connect the light guide to be connected
to the instrument to the target system by its output first to prevent a hazard to the user from the
high-energy light emitted by the light source.
The light guide (Storz standard) is inserted into
the provided microscope adapter (Fig. 10.2) or
into the external DMi8 fluorescence axis as far
as it goes and fastened using a clamping screw.
Before that, fasten the adapter on the standard
interchangeable surface of the fluorescence
lamp housing. (Upright microscope – directly on
the stand, inverted microscope – on the lamp
housing mount, stereomicroscope – directly on
the stand). The light source has a safety interlock
Fig. 10 Light guide with adapter
1 Light guide
2 Adapter
1
1
24
2
7. Instrument Setup
for the light guide that prevents light output unless a liquid light guide is fully inserted into the
light guide port. Before operating the unit, make
sure the 3 mm diameter liquid light guide is properly installed in the light guide port.
The set screw should be loosened using a 2 mm
hex wrench so the light guide slides all the way
into the receptacle without obstruction. Once
the light guide is fully inserted, lightly tighten the
set screw to hold it in place and prevent inadvertent disconnection.
The input of the light guide (Storz long) is inserted
into the light output on the front side of the light
source into the light output (Fig. 11.1, Fig. 12.1) of
the instrument. There must be a noticeable click.
!
Attention!
When connecting the light guide to the light
source or the microscope adapter, make sure not
to kink or damage the light guide. Avoid turning
the clamping screw too far. Make sure that the
light guide is securely in place to prevent it from
sliding out of the adapter.
Only use a light guide with a light input of the
"Storz long" type, as otherwise damage to the instrument and hazard to the user can result (risk
of being dazzled).
Fig. 12 LED5/8
1 Output adapter
1
Fig. 13 LED1 Connections
1 USB A connection
Fig. 11 LED3
1 Output adapter
1
1
25
7. Instrument Setup
7.4
Attention!
Connect the light guide on either side (light
source AND adapter) before switching on the
light source!
The escaping light can cause injury to eyes
and skin and damage to furniture.
Never look into the light escaping from the
light guide!
Connecting the Leica POD (optional)
As an alternative the light source can be connected to Leica POD.
LED1
Connect the USB A port of the light source control pod (Fig. 14.1) accessory to the USB A (“external”) port on the LED1 (Fig. 13.1) using the
USB-A to-USB A cable.
LED5/8
CAUTION
Do not switch on the light source until the
light guide is firmly connected to the microscope.
Uncontrolled light output from the light guide
poses a burning hazard at the light guide output in addition to the blinding hazard!
Connect LED5/8 to pod using USB A-to-USB B
cable.
7.5
Connecting the power cable
The power cable is a standard C13 cable included in the standard delivery.
Connect the DC power cable to the DC power input of the light source.
Before connecting the power cable, the power
switch of the light source must be set to zero position (OFF).
Fig. 14 Leica POD
1 Connection to light source
2 Connection to PC
1
26
2
The power cable must first be connected with
the external power supply and then inserted
into a properly functioning grounded socket.
The ground contact connection must not be interrupted (e.g. by unsuitable extension cables).
8. Operation
8. Operation
8.1
first turned on, and then increased as necessary.
A yellow indicator light (Fig. 15.4) below the INTENSITY control knob on the side panel alerts the
user that light output is active.
Operation LED1
Two control knobs are located on the side panel
(Fig. 15.2). The “COLOR” knob allows the user to
select light output from any or all of the four color
channels. The selections are “V” for violet, “C”
for cyan, “G” for green and “R” for the red color
channel. The “W” setting is for white light, when
all four color bands are simultaneously illuminated. The “ext” setting allows the light source
to be controlled using the Pod. There are two
“off” settings at opposite ends of the knob range.
Both of these “off” settings turn the electrical
power to the light engine off. The “INTENSITY”
control knob (Fig. 15.3) sets four different levels
of output attenuation between 100% and 10% of
maximum. This setting is applied to the current
source selection according to the position of the
“COLOR” knob. It is recommended that the intensity be set to the “10%” position when a source is
Fig. 15 LED1
1 Output adapter
2 On/Off and color switch
3 Intensity switch
4 Light indicator
8.2
Operation LED3
The rocker switch in the top left corner of the
rear panel controls electrical power to the unit.
A green LED above the switch indicates that
power to the light engine is ON.
Refer to Fig. 16.4 for the rear panel locations of
the input DC power connection, the foot pedal
connection and the master electrical power
switch. Note that the foot pedal is an on/off
toggle switch. Its on/off status cannot be determined from its position.
Before connecting the foot pedal, make sure the
electrical power switch (Fig. 16.1) is in the OFF
position to avoid unintentional initiation of light
output. After connecting the foot pedal, and with
the light guide output safely directed into an enFig. 16 LED3 rear
1 Power indicator light
2 On/Off switch
3 Foot switch
4 Receptacle DC power supply
5 Shutter
1
2
2
3
4
1
3
5
4
27
8. Operation
closed optical path (e.g. microscope input collimator or a beam dump), turn the power switch
ON to begin operation.
The LED3 light output can be turned on or off using the manual light output switch on the front
panel or using the foot pedal toggle switch accessory. Do not intersperse the operation of
these switches. If the front panel rocker switch is
used to turn the light on, that same switch should
be used to turn it off. If the foot pedal is used to
turn the light on, the foot pedal be used to turn
the light off. An amber indicator LED above the
manual light output switch indicates active light
ouput. There is no warm-up time; the light engine
output stabilizes less than 1 second after the
manual light output switch is moved to the “on”
position.
Fig. 17 LED3 front
1 Light indicator
2 Light switch
3 Output adapter
1
2
3
28
Light output can be switched off during intervals
when it is not required for active viewing or data
collection. After light output is switched off, the
cooling fan will continue to run for 5 minutes,
after which it will automatically stop until light
output is turned back on. Light output is set to a
single maximum intensity level. LED3 do not provide electronically-controlled output attenuation.
In the event that the liquid light guide becomes
disconnected from the unit during operation, the
safety interlock will be opened and light output
will cease immediately. In order to turn the light
output back on, you will need to: 1) turn the electrical power off, 2) fully insert the light guide into
the light guide port, 3) turn the electrical power
back on and then 4) activate light output using
either the front panel rocker switch or the foot
pedal toggle switch.
8. Operation
8.3
Operation LED5/8
The Master Power Switch button on the front
panel (Fig. 18.8) turns the electrical power to the
unit on or off. A green power indicator embedded
in the button is lit when the power supply is connected to the light engine and the power button
is in the on position. Initialization of the onboard
computer takes about 30 seconds after the master power switch is turned on. When initialization is complete, the status indicator display (Fig.
18.1) will activate
Fig. 18 LED5/8 front
1 Status indicator display
2 Light indicator
3 Interlock connection
4 TTL connection
5 USB B connection
6 RS-232 connection
7 Receptacle DC power supply
8 On/Off switch
9 Key control
10 Output adapter
11 Manual interlock reset
The Key Control must be in the on position before
light output can be turned on. The key must be
removed and stored in a secure location when
the product is not in use. ONLY trained individuals should use and have access to the key. The
Master Power Switch button, Key control and
Remote interlock can be used to shut off light
output.
The Source Enabled indicator LED (Fig. 18.2) provides a warning indication that one or more light
sources are active and emitting invisible and/or
visible radiation.
Fig. 19 LED5/8 rear
1
8
2
3
9
4
5
10
6
7
11
29
8. Operation
The Remote Interlock Connector (Fig. 18.3) is provided to allow for connection of a remote interlock. When this interlock is open it will shut off
light output. After the interlock has been opened,
the Manual Interlock Reset (Fig. 18.11) button will
need to be pushed to resume light output.
Note: In the event of ANY normal or abnormal interlock fault condition (including high ESD/EMP/
EFT conditions ~2kV) you MUST clear the latch
fault condition by depressing the manual reset
button.
8.3.1 Start Up
• Insert the external gate jumper in the labeled
socket on the front panel (Fig. 18.3).
• Insert the control key (Fig. 18.9), turn it to the
ON position.
• As soon the DC power supply is energized, the
master power button (Fig. 18.8) will automatically light up. The light engine automatically
starts when the power is connected; there is
no need to push the master power button.
• Wait 30–45 seconds for the initiation sequence
(onboard microprocessor boot-up) to complete. Do not press any buttons or insert any
plugs during this time.
30
• When initiation sequence completes, LEICA
flashes on the front display panel. The display
will then show the MODEL identification (LED5
or LED8), the internal temperature and fan status. . At the same time, the fan will come on at
HI for about 2 seconds and then shut off automatically.
• The light source is now ready for use.
The light source has two serial ports, labeled
POD and PC (Fig. 18.5, Fig. 18.6), which can be set
to receive either LEGACY or STANDARD mode
commands. Connection to the computer requires
a USB-A-to-USB B cable or USB-to-RS-232 cable.
The COM port address assigned by the computer
to the light engine USB serial port must be correctly registered. These selections are typically
found under the “Devices” tab in the Leica LAS
X software.
9. Service
9. Service
9.1
Maintenance and cleaning
The light source has no need of regular maintenance. This does not void the obligation of carrying out legally controlled regular checks with regard to electrical safety and accident prevention.
9.2
Repairs
Repairs may be carried out by the manufacturer
of the instrument or specially authorized representatives only.
Should the necessity arise to send the light
source to the vendor or its authorized representative in case of a defect, the original packaging
of the instrument must be used.
Attention!
Before carrying out cleaning tasks, switch off
the light source.
Use only a dry, lint-free cloth for cleaning. When
cleaning, ensure that no dust or other foreign
bodies enter the instrument through the ventilation slots.
In case of damage (even external damage), immediately stop using the light source and send it
to Service.
If it will not be used for a long time, protect the
light source from dust using a suitable cover
(such as the plastic bag from the original packaging).
31
10. Troubleshooting
10. Troubleshooting
No routine maintenance is required. There are no user-replaceable components or sub-assemblies
in the light source. Opening the light engine enclosure will void the manufacturer’s warranty. If problems are encountered during installation or routine operation, review the troubleshooting procedures
described below. If the problem remains unresolved, please contact your local Leica sales representative for assistance.
Troubleshooting Procedures
Problem
Check the following
No response to TTL trigger Check that TTL trigger inputs are polarity positive, and that serial
commands
ON/OFF controls (PC or Control POD) are set to OFF state.
No response to TTL trigger The light output must be turned ON by either the manual light output
commands (LED1/3)
switch (LED1 and LED3) or by the Leica POD (LED1) BEFORE applying
the TTL control signal to the shutter.
No light output in response There are 3 interlocks that need to be closed, check all of these:
to source ON command (se- •
Liquid light guide must be inserted in light output receptacle.
rial or TTL). (LED5/LED8 only) •
External gate jumper must be inserted in front panel
•
Control key must be inserted in front panel and turned to “on”
position
Unusually weak fluores- Weak fluorescence in all detection channels (DAPI, FITC, TRITC,
cence signals across all de- Cy5 etc) is likely to be due to poor light transmission by the liquid
tection channels
light guide, the collimating adaptor or another distal component of
the microscope optical path and not to abnormally low light output
from the light source.
Unusually weak fluores- •
cence signals in a single detection channel (e.g. DAPI)
Check that the dichroic beamsplitter and emission bandpass
filter in the microscope are compatible with light engine excitation filter specifications shown on the certificate of conformance.
High, spatially uniform fluo- Check that the dichroic beamsplitter and emission bandpass filter
rescence background
in the microscope are compatible with light engine excitation filter
specifications shown on the certificate of conformance (Figure 1).
Green power indicator does Check that the liquid light guide is fully inserted in the front panel
not light up when the DC receptacle and is secured by the set screw.
power supply is connected and the master power
switch (rear panel) is in the
ON position. (LED3 only)
32
11. EC Declaration of Conformity
11. EC Declaration of Conformity
To download the EC Declaration of Conformity, use this link
http://www.leica-microsystems.com/products/light-microscopes/
Select the LED Light Source and go to the "Download" page.
If you have any further questions or require technical support, please contact your country's Leica
branch office or your local contact person directly. The appropriate contacts can be found on the
Internet under:
http://www.leica-microsystems.com
33
www.leica-microsystems.com
Copyright © Leica Microsystems CMS GmbH · Ernst-Leitz-Straße · 35578 Wetzlar · Germany 2019
Phone +49-6441-29-0 · Fax +49-6441-29-2599 LEICA and the Leica logos are registered trademarks of Leica IR GmbH.
Order nos. of the editions in: English/German/French 11934173 · Printed on chlorine-free bleached paper.
Subject to modification
Revision 1.0, 2019-05-01
Document Number 57-10022 Revision A
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