Leica Microsystems | DM750 M | User manual | Leica Microsystems DM750 M Upright Microscopes User manual

Leica Microsystems DM750 M Upright Microscopes User manual
Leica DM750 M
User Manual
Manufacturer Information
Published in October 2019 by:
Leica Microsystems (Schweiz) AG
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
In case of questions, please contact the hotline:
sms.support@leica-microsystems.com
Leica DM750 M User Manual
2
Chapter Overview
Safety Regulations
Assembling the Leica DM750 M
5
16
Use25
Conversion and Use with Transmitted Light
32
Care of the Microscope
46
Leica DM750 M User Manual
3
Contents
Manufacturer Information
Chapter Overview
Safety Regulations
Safety Concept
Symbols Used in this Instruction Manual
Important Notes
Instructions on Use
Health Risks and Dangers of Use
Information for the Person Responsible for the Instrument
Care Instructions
Accessories, Maintenance and Repair
Electrical Data and Ambient Conditions
2
3
6
7
8
9
11
12
13
14
15
Assembling the Leica DM750 M
Assembling the Incident Light Axis
17
Assembling the Viewing Tubes
18
Leica EZ Viewing Tube with Integrated Eyepieces
19
Leica Standard Tube with Separate Eyepieces
21
Eyecups22
Installing Objectives
23
Conversion to Incident Light Polarization
24
Leica DM750 M User Manual
Use
Switching on the Microscope
Modifying the Aperture Diaphragm
Illumination Scenarios
Automatic Shutoff of Illumination
Using Specimen Holders
26
27
28
30
31
Conversion and Use with Transmitted Light
Optional Koehler Illumination
33
Installing the Condenser
34
36
Switching on the Microscope
Complete Condenser Centering 37
Using the Condenser
38
Preparation for Viewing a Specimen Slide
39
Focusing40
Viewing Tube Adjustment
41
Koehler Configuration 44
Care of the Microscope
General Maintenance
47
4
Safety Regulations
Leica DM750 M User Manual
Safety Regulations5
Safety Concept
The individual modules of the Leica DM microscopy series include an interactive CD-ROM with
all relevant user manuals in several languages.
Keep it in a safe place, and readily accessible
to the user. User manuals and updates are also
available for you to download and print from
our website at www.leica-microsystems.com.
This user manual describes the special functions of the individual modules of the Leica
DM microscopy series and contains important
instructions for their operational safety, maintenance, and accessories.
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety requirements of the
supplier.
Before installing, operating or using the instruments, read the user manuals listed above. In
particular, please observe all safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
The "Safety concept" booklet contains additional safety information regarding the service
work, requirements and the handling of microscope, accessories and electrical accessories as
well as general safety instructions.
Leica DM750 M User Manual
Safety Regulations6
Symbols Used in this Instruction Manual
Warning of a danger
This symbol indicates especially important information that is mandatory to
read and observe.
Failure to comply can cause the following:
Hazards to personnel
OO
OO
Functional disturbances
instruments
or
damaged
Warning of hazardous electrical voltage
This symbol indicates especially important information that is mandatory to
read and observe.
Failure to comply can cause the following:
OO Hazards to personnel
OO
Danger due to hot surface
This symbol warns against touching
accessible hot surfaces, e.g. those of
light bulbs.
Important information
This symbol indicates additional information or explanations that intend to
provide clarity.
Explanatory notes
ff This symbol within the text points to additional information and explanations.
Instructions for disposing of the
instrument, accessory components,
and consumables.
Functional disturbances or damaged
instruments
Leica DM750 M User Manual
Safety Regulations7
Important Notes
Description
The Leica DM750 M microscope meets today's
state of the art of technology. Nevertheless,
hazards may still arise during operation. The
potential risks are described below.
Before installing, operating or using the
instrument, it is mandatory to read this
user manual. In particular, please observe all
safety instructions.
User manual
This user manual includes important instructions related to operating safety, maintenance
and accessories.
Your Leica DM750 M microscope comes
with an interactive CD-ROM with all
relevant user manuals. Keep it in a safe place,
and readily accessible to the user. User manuals and updates are also available for you
to download and print from our website at
www.leica-microsystems.com.
Accessories from third-party suppliers
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety requirements of the
supplier.
Original condition
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Legal requirements
Adhere to general and local regulations relating to accident prevention and environmental
protection.
EC Declaration of Conformity
Electrically
operated
accessories
are
constructed based on the state of the art of
technology and are provided with an EC Declaration of Conformity.
Leica DM750 M User Manual
Safety Regulations8
Instructions on Use
The Leica DM750 M microscope may be
used only in closed rooms and must be
placed on a solid substrate.
Always position the Leica DM750 M
microscope so that you can disconnect it from the power supply at any time.
The power cable must remain accessible at all
times, because it is intended as a power disconnect device.
Place of use
Only use the instruments in closed, dust free
rooms and between +10°C and +40°C. Protect
the devices from oil, chemicals and extreme
humidity. If using the devices outdoors, protect
them from dust and moisture. Never use electrical devices outdoors. Install electrical devices at
least 10 cm from the wall and away from flammable substances.
Non-intended use
Never install any other plug or unscrew
any mechanical components unless
expressly instructed to do so in the instructions.
The devices and accessories described in
this instruction manual have been tested
for safety and potential hazards.
Avoid large temperature fluctuations, direct
sunlight and vibrations.
In warm and warm-damp climatic zones,
the individual components require
special care in order to prevent the build-up of
fungus.
Leica DM750 M User Manual
Safety Regulations9
Instructions on Use (Continued)
The responsible Leica affiliate must be
consulted whenever the instrument is
altered, modified or used in conjunction with
non-Leica components that are outside of the
scope of this manual!
Unauthorized alterations to the instrument or noncompliant use shall void all
rights to any warranty claims.
Transport
If at all possible, use the original packaging for
shipping or transporting individual modules.
Disposal
Once the product has reached the end of its
service life, please contact Leica Service or Sales
about disposal.
Please observe and ensure compliance with the
national laws and regulations that implement,
for example, the EC Directive WEEE.
Integration in third-party products
When installing Leica products into third-party
products, the manufacturer of the complete
system or its dealer is responsible for following all applicable safety instructions, laws and
guidelines.
Like all electronic devices, this instrument, its accessory components
and consumables must never be disposed of
with general household waste. Disposal must
comply with locally applicable laws and regulations.
In order to prevent damage from vibrations,
disassemble all moving parts that (according to
the user manual) can be assembled and disassembled by the customer and pack them separately.
Leica DM750 M User Manual
Safety Regulations10
Health Risks and Dangers of Use
Health risks
Workplaces with microscopes facilitate
and improve the viewing task, but they
also impose high demands on the eyes and
holding muscles of the user. Depending on the
duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken:
OO
Optimum workplace layout
OO
Frequent changes of activity
OO
Thorough training of the personnel, giving
consideration to ergonomic and organizational aspects
Leica DM750 M User Manual
The ergonomic design and construction of the
Leica microscopy series are intended to reduce
the exertion of the user to a minimum.
Dangers during use
OO The Leica DM750 M microscope may only
be connected to a grounded socket.
Danger of infection
Direct contact with eyepieces is a
potential transmission method for
bacterial and viral infections of the eye.
OO
The risk can be kept to a minimum by
using personal eyepieces for each individual or detachable eyecups.
The Leica DM750 M microscope may not be
operated unless it is in proper functioning
condition.
The microscope illumination is in the exempt
group (risk group 0) according to EN 62471:2008
when used according to its intended use.
• of the illumination equipment – either
Never look directly into the LED beam
with or without optical instruments – as this
increases the risk class. Failure to observe this
notice poses a risk of eye damage.
Safety Regulations11
Information for the Person Responsible for the Instrument
Information for the person responsible for
the instrument
OO Ensure that the Leica DM750 M microscope
is used only by qualified personnel.
OO
Ensure that this user manual is always available at the Leica DM750 M microscope.
OO
Carry out regular inspections to make
certain that the authorized users are adhering to safety requirements.
OO
OO
OO
Do not use the Leica DM750 M microscope
unless it is in perfect condition.
OO
Only original Leica replacement parts may
be used in servicing the product.
OO
Inform your Leica representative or
Leica Microsystems (Schweiz) AG,
9435 Heerbrugg, Switzerland, immediately
of any product defect that could potentially
cause injury or harm.
OO
After service work or technical modifications, the unit must be reconfigured with
observance to our technical requirements.
OO
If the unit is modified or serviced by unauthorized persons, is improperly maintained
(as long as maintenance was not carried
out by us), or is handled improperly, Leica
will not accept any liability.
OO
The electric installation in the building
must conform to the national standard, e.g.
current-operated ground leakage protection (fault-current protection) is suggested.
OO
When instructing new users, do so thoroughly and explain the meanings of the
warning signs and messages.
Assign individual responsibilities for starting, operating and servicing the instrument and monitor the observance of these
responsibilities.
Leica DM750 M User Manual
OO
If you use accessories made by third-party
manufacturers with the Leica DM750 M
microscope, be sure that each such manufacturer confirms the safety-engineering,
harmless usability of the product and
observe the product's user manual.
Modifications and maintenance of the
Leica DM750 M microscope may only be
performed by professionals expressly
authorized by Leica.
Safety Regulations12
Care Instructions
General instructions
OO Protect the Leica DM750 M microscope
against damp, vapors, acids, alkalis, and
corrosive substances. Do not store chemicals in the vicinity.
OO
Protect the Leica DM750 M microscope
from oil and grease. Never grease or oil
mechanical parts or sliding surfaces.
OO
Follow the instructions of the disinfectant
manufacturer.
OO
It is advisable to enter a service agreement
with Leica Service.
Leica DM750 M User Manual
Cleaning coated parts and plastic parts
Dust and dirt particles should be removed
with a soft brush or lint-free cotton cloth.
OO
OO
Remove coarse debris with a moistened
disposable cloth.
OO
Acetone, xylene or nitro-containing thinners must NOT be used.
OO
Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces,
and specimens could be contaminated by
abraded particles.
Cleaning glass surfaces
Remove dust using a dry and grease-free
brush made from hair, by blowing with
bellows, or by using a vacuum.
OO
OO
Optical surfaces should be cleaned with a
lint-free cloth, lens tissue, or cotton swab
moistened with a commercially available
glass cleaner.
Safety Regulations13
Accessories, Maintenance and Repair
OO
The Leica accessories described in this user
manual.
Maintenance
The Leica DM750 M microscope is basically
maintenance-free. To ensure that it always
operates safely and reliably, we recommend
that you take the precaution of contacting
the responsible service organization.
OO
Other accessories, provided that these have
been expressly approved by Leica as being
technically safe in this context.
You can arrange for periodic inspections
or, if appropriate, conclude a maintenance contract with them.
Accessories
Only the following accessories may be used
with the Leica DM750 M microscope:
Leica DM750 M User Manual
OO
OO
It is advisable to enter a service agreement
with Leica Service.
OO
For maintenance and repair, only OEM
spare parts may be used.
Repairs and service work
OO Only original Leica Microsystems spare
parts may be used.
OO
Before opening the instruments, switch off
the power and unplug the power cable.
OO
Avoid contact with powered electrical
circuits, which can lead to injury.
Service address
In case of problems, please contact us as
follows:
stereo.service@leica-microsystems.com
Safety Regulations14
Electrical Data and Ambient Conditions
Fuse replacement
Unplug the instrument before changing any fuses. The
Leica DM750 M includes two fuses that are located behind
the power cable compartment.
Only use the following fuse types: 5×20 mm, 1 A/250 V,
fast-acting fuse (# 13RFAG30003)
Electrical data
Input: 100–240 V, 50/60 Hz, 5 W (3 W LED)
Environment
Temperature for use
+10 °C … +40 °C
Storage temperature
-20 °C … +52 °C
+50 °F … +104 °F
Manipulation shock
25 mm on 50 mm hard wood
Transport shock (unpacked)
100 g / 6 ms
Transport shock (packed)
800 mm free fall
Transport vibrations (unpacked)
5–200 Hz / 1.5 g
Air pressure during use and storage
500–1,060 mbar
Humidity during use and storage
20–90 %
Installation Category II (Overvoltage Category)
Pollution degree 2
Leica DM750 M User Manual
Safety Regulations15
Assembling the Leica DM750 M
Leica DM750 M User Manual
Assembling the Leica DM750 M16
Assembling the Incident Light Axis
Assembly
1. Place the incident light axis with the heat
sink facing backwards onto the tripod
mount.
3. Fasten the incident light axis into place by
tightening the setscrew.
2. Align the incident light axis according to
the tripod geometry.
Leica DM750 M User Manual
Assembling the Leica DM750 M17
Assembling the Viewing Tubes
There are two types of viewing tube.
Identify which viewing tube you have:
Assembly
1. Loosen the setscrew (on top of the stand)
using the Allen key provided.
2. Set the dovetail into the tripod mount and
tighten the setscrew carefully. This way,
the tube automatically shifts to the correct
position on the optical axis of the microscope.
Type 1: Leica EZ viewing tube with integrated
eyepieces
Type 2: Standard tube with separate eyepieces
Leica DM750 M User Manual
Assembling the Leica DM750 M18
Leica EZ Viewing Tube with Integrated Eyepieces
1. In order to use the wingscrew, remove the
setscrew that was delivered with the stand.
3. Reinstall the Leica EZ viewing tube onto the
stand.
2. Detach the wing screw part completely
before the screw is used on the stand.
4. Tighten the wingscrew using the wrench
included in the delivery package.
Leica EZ viewing tube with integrated eyepieces
Loosen the setscrew at the stand or
replace the setscrew with the optional
wingscrew to rotate the Leica EZ viewing tube.
Leica DM750 M User Manual
Assembling the Leica DM750 M19
Leica EZ Viewing Tube – Integrated Eyepieces (Continued)
Now, you can rotate the Leica EZ viewing
tube by loosening the wingscrew, bringing the tube to the desired position and tightening the wingscrew again.
The eyepieces are integrated into the
Leica EZ Viewing Tube and preset; therefore, there is no need to adjust or install the
eyepieces.
Continue with the "Eyecups" section on
page 22.
Leica DM750 M User Manual
Assembling the Leica DM750 M20
Leica Standard Tube with Separate Eyepieces
1. Insert the eyepieces into the tubes.
2. Secure the eyepieces in the tubes by tightening the silver screws at the bottom.
Standard tubes; tubes do not include eyepieces
yet
The standard tube includes a rotatable
dovetail. Therefore, you can now rotate
the standard viewing tube freely in any orientation.
Leica DM750 M User Manual
The eyepieces can still be rotated, but no
longer detached from the tubes.
Assembling the Leica DM750 M21
Eyecups
If you wear eyeglasses for microscope
viewing, keep the rubber eyeguards
folded down. If you do not wear eyeglasses,
you may find it useful to unfold the rubber
eyeguards in order to help block out ambient
room light.
Leica DM750 M User Manual
If you have purchased a standard microscope configuration, the objectives are
pre-installed on the objective nosepiece and
the specimen stage condenser is installed
onto the stand. In this case, continue with the
"Use" section starting on page 25. If you have
purchased your Leica DM750 M in individual
components and not according to the standard
configuration, continue with the "Installing
Objectives" section on page 23.
Assembling the Leica DM750 M22
Installing Objectives
Installing objectives
When rotating the objective nosepiece,
always use the knurled ring on the
objective nosepiece.
While rotating the objective nosepiece clockwise, screw the objectives into the objective
nosepiece. Start with the lowest magnification.
Leica DM750 M User Manual
Assembling the Leica DM750 M23
Conversion to Incident Light Polarization
1. Insert the polarizer into the polarizing
adapter.
3. Push the analyzer into the opening as far as
it will go.
2. Remove the cover of the analyzer.
4. For reflected light, be sure beam splitter
slider is pushed completely in.
When using transmitted light only, pull
beam splitter slider to the out position.
This will automatically shut off the reflected light to save energy and avoid any false
pleochroism effect that could be caused by
the beam splitter.
Leica DM750 M User Manual
Assembling the Leica DM750 M24
Use
Leica DM750 M User Manual
Use25
Switching on the Microscope
Precautionary measures
The light of the Leica DM750 M can be
very bright. Therefore, only look into
the eyepieces after you have switched on and
reduced the illumination (see item 3)!
Connecting and switching on the microscope
1. Connect the USB cable from the incident light axis to the 5 V/1.5 A USB power
connector on the rear of the Leica DM750 M
Stand. If you have a stand without a USB
power connector, then connect the USB
cable to an external USB power supply.
Leica DM750 M User Manual
2. Switch on the illumination by briefly pressing the power switch.
The brightness can be adjusted in 15
increments.
3. Look through the eyepieces and adjust the
brightness to the desired level.
Use26
Modifying the Aperture Diaphragm
Aperture diaphragm
The aperture diaphragm of the incident
light axis can be opened or closed. An
open aperture diaphragm generates a higher
resolution with a decreased depth of field. A
closed aperture diaphragm, on the other hand,
reduces the quantity of light and resolution
capacity, but the depth of field is increased.
Opening / closing the aperture diaphragm
1. Close the aperture diaphragm by moving
the lever upwards.
2. Open the aperture diaphragm by moving
the lever downwards.
The aperture diaphragm must be
completely open for all oblique light illumination scenarios.
Leica DM750 M User Manual
Use27
Illumination Scenarios
Illumination scenarios
You can adjust the incident light axis
illumination to your needs and requirements using the membrane control panel.
Changing the illumination mode
Press the button in the middle once to switch
between bright-field illumination and oblique
illumination (oblique incident illumination).
Individual light field (oblique light)
Hold a button pressed longer than two seconds
to activate only one of the four light fields for
oblique illumination. Combinations with other
light fields are also possible.
The aperture diaphragm must be
completely open for all oblique light illumination scenarios.
HOLD
Bright field illumination
Oblique illumination
Leica DM750 M User Manual
Individual light field
Use28
Illumination Scenarios (Continued)
Adding light fields
Press a button only briefly to switch an additional light field on or off.
Switching to bright field
Press the middle button briefly to toggle
between bright-field illumination and the
previously selected oblique light configuration.
Automatically calling up
oblique light illumination scenarios
Hold the middle button pressed longer than
two seconds; as long as you do not release the
button, the next light field is activated every
two seconds.
HOLD
Any arrangement
Leica DM750 M User Manual
Switching between bright field illumination
and the most recent setting
Switching automatically
between the light fields
Use29
Automatic Shutoff of Illumination
The illumination of the Leica DM750 M
is shut off automatically if the user does
not make any modifications for two hours. This
setting can be changed.
Deactivating delayed shutoff
Activating delayed shutoff
1. Switch the incident light axis off.
1. Switch the incident light axis off.
2. Hold the
button pressed while switching on the incident light axis.
2. Hold the
button pressed while switching on the incident light axis.
After the delayed shutoff is deactivated,
the LED flashes three times slowly and
then stays steady.
After the delayed shutoff is activated, the
LED flashes three times quickly and then
stays steady.
If the incident light illumination is
disconnected from the power supply and
then reconnected, automatic shutoff is always
activated (factory setting).
When the beam splitter slider is moved
to the out position, this will automatically shut off the reflected light to save energy
and avoid any false pleochroism effect that
could be caused by the beam splitter.
Leica DM750 M User Manual
Use30
Using Specimen Holders
Additional specimen holders
Specimen holders are available in two
different versions, with a diameter of
25 mm and 30 mm:
OO
Order number for 25 mm version:
13 613 167
OO
Order number for 30 mm version:
13 613 168
The maximum height of the specimen is
30 mm.
Leica DM750 M User Manual
Inserting the specimen holder
1. Unscrew the ring from the specimen holder.
4. Set the specimen holder into the stage
aperture and push it to the rear as far as it
will go.
2. Insert the specimen.
3. Screw the ring onto the specimen holder.
5. Position the specimen by moving the stage
and looking through the eyepieces simultaneously.
Use31
Conversion and Use with
Transmitted Light
Leica DM750 M User Manual
Conversion and Use with Transmitted Light32
Optional Koehler Illumination
Transmitted illumination
For the Leica DM750 M, there are two
condensers available for optimum transmitted illumination. Determine which type of
illumination is used for your instrument since
this will be important later.
Leica DM750 M User Manual
Type 1: Standard condenser
Adjustable condenser centering with tool kept
at the ready:
Type 2: Koehler condenser
Adjustable
condenser
centering
with
wingscrews and adjustable Koehler field
diaphragm:
Conversion and Use with Transmitted Light33
Installing the Condenser
Condenser
The Leica DM750 M is equipped with
an open holder for the specimen stage
condenser; in other words, the condenser must
be installed.
The holder of the condenser is open
Leica DM750 M User Manual
1. Move the specimen stage upwards as far as
it will go using the coarse focusing knob.
3. Unscrew the two setscrews (or wingscrews
for Koehler stands) on the condenser
holder.
2. Move the condenser holder into the lowest
position using the condenser focusing
knob.
Conversion and Use with Transmitted Light34
Installing the Condenser (Continued)
4. Push the condenser under the specimen
stage into the holder by aligning the locating pin on the bottom of the condenser
with the slot on the rear side of the fork.
5. Move the condenser into the highest position using the condenser focusing knob.
6. Tighten the two setscrews (Koehler stand:
wingscrews) so that the upper lens of the
condenser is centered under the objective in working position and the specimen
stage condenser is thus roughly centered.
Leica DM750 M User Manual
How to center the condenser more
precisely is described in the section
entitled "Complete Condenser Centering" on
page 37.
Conversion and Use with Transmitted Light35
Switching on the Microscope
Precautionary measures
The light of the Leica DM750 M can be
very bright. Therefore, do not look into
the eyepieces until after you have switched on
the illumination!
USB power connector
The Leica DM750 M has a 5 V/1.5 A USB power
connector in the center of the cord wrap. This
can be used to power some Leica cameras,
incident illuminator, or other devices requiring
5 V/1.5 A.
Leica DM750 M User Manual
Connecting and switching on the microscope
1. Plug the power cable of the microscope
into a corresponding grounded socket.
2. Reduce the brightness to the minimum.
3. Switch on the microscope using the switch
at the bottom right of the microscope
stand.
4. Look through the eyepieces and adjust the
brightness to the desired level.
Conversion and Use with Transmitted Light36
Complete Condenser Centering
Complete condenser centering
1. Open the condenser aperture by rotating
the knurled ring on the condenser to the
right.
2. Write an "X" on a piece of paper in the size
of a business card and place it onto the
light output of the microscope stand in
such a way that the "X" is centered over the
illumination.
Not centered
Make sure that the condenser is in the
highest position.
Leica DM750 M User Manual
3. Look through the eyepieces and center the
"X" in the field of view by tightening the
setscrews. (Leica DM750 M with Koehler
illumination: tighten the knurled screws).
Centered
Conversion and Use with Transmitted Light37
Using the Condenser
Using the condenser
The condenser is furnished with an iris
diaphragm, which can be adjusted to
match the effective numerical aperture of the
objective.
1. To open and close this diaphragm, simply
turn the knurled ring on the condenser to
the right or to the left so that the line on the
ring is aligned with the objective magnification used.
2. Open the iris diaphragm of the condenser
completely by turning the condenser ring
all the way to the right.
The line on the ring is aligned with the objective
magnification used.
Leica DM750 M User Manual
Conversion and Use with Transmitted Light38
Preparation for Viewing a Specimen Slide
1. Position a specimen slide on the specimen
stage by sliding it under the slide grips.
2. Position the specimen slide such that a
part of the specimen is under the objective
used.
Slide grips hold the slide in place.
Slide grips
Leica DM750 M User Manual
Conversion and Use with Transmitted Light39
Focusing
1. Rotate the objective nosepiece in such a way
that the objective with the lowest magnification level is in the working position.
2. Move the specimen stage upwards by turning the coarse focusing knob as far as it will
go to the maximum position.
3. Look into the eyepieces and adjust the illumination intensity to the desired level.
The stand of the Leica DM750 M has been
calibrated at the factory so that the focus
can be found from this position within 1.5 rotations of the fine focus.
4. Bring the specimen into focus using the
fine focusing knob.
Leica DM750 M User Manual
Conversion and Use with Transmitted Light40
Viewing Tube Adjustment
Adjust the eyetubes
1. Adjust the tubes to your interpupillary
distance. Fold or unfold the eyetubes to
decrease or increase the distance between
the eyepieces until you see one illuminated
circle.
Leica EZ viewing tube
OO If you are using a Leica EZ viewing tube,
which has eyepieces that are integrated
into the tubes, no further adjustments
are required. Simply wear your glasses or
contact lenses.
OO
Leica DM750 M User Manual
For Leica DM750 M with Koehler field
diaphragm, continue with the "Koehler
Configuration" section on page 44.
Standard viewing tube with two fixed
eyepieces
OO If you are using a standard tube with two
fixed eyepieces (no focusing eyepieces), no
further adjustments are required. Simply
wear your glasses or contact lenses.
OO
For Leica DM750 M with Koehler field
diaphragm, continue with the "Koehler
Configuration" section on page 44.
Conversion and Use with Transmitted Light41
Viewing Tube Adjustment (Continued)
Standard tube
If you are using a standard viewing tube with
one or two focusing eyepieces, you need to
make some adjustments.
2. Bring the specimen into focus using the fine
focus setting knob while looking through
only one of the eyepieces. Cover your other
eye.
1. Set the focusing eyepieces to "0".
If you are comfortable wearing your
corrective lenses (contact lenses or
eyeglasses) for microscope viewing, leave them
on and your adjustments will be minimal.
Leica DM750 M User Manual
3. Now look with the other eye just through
the other eyepiece (focusing eyepiece).
Bring the specimen into focus using the
focus function of the eyepiece.
When doing so, do not change the
height of the specimen stage.
If you are using one focusable and one
non-focusable eyepiece, look through
the non-focusable eyepiece.
4. Grip the knurled ring on the focusing
eyepiece with one hand and rotate the top
of the eyepiece with the other hand until
the specimen is in focus for this eye and
this focusing eyepiece. This corrects for any
vision differences between your right eye
and left eye.
Conversion and Use with Transmitted Light42
Viewing Tube Adjustment (Continued)
5. Now, switch to an objective with a high
magnification level and bring the microscope into focus while looking through the
eyepieces with both eyes.
The depth of field is lower for higher
magnification levels. Thus, you will
notice that if you switch to low magnification
after bringing the microscope into focus with
great magnification, a minor adjustment of the
fine focus may be required.
OO
If your DM750 M is equipped with a Koehler configuration, continue with the next
section entitled "Koehler Configuration" on
page 44.
Leica DM750 M User Manual
Conversion and Use with Transmitted Light43
Koehler Configuration
If your Leica DM750 M is equipped with a field diaphragm for
Koehler illumination, center the condenser as described below.
2. Focus the illuminated field diaphragm using the condenser focusing
knob on the left side of the stage mount.
1. Adapt the Koehler field diaphragm to the base of the microscope so
that the illuminated field diaphragm is in the field of view when you
look through the eyepieces.
Condenser focusing knob
Close the Koehler field diaphragm
Leica DM750 M User Manual
Illuminated field diaphragm is
focused
Closed Koehler field diaphragm
Conversion and Use with Transmitted Light44
Koehler Configuration (Continued)
3. Turn the condenser centering wingscrews simultaneously to center
the image of the field diaphragm.
Leica DM750 M User Manual
4. Open the field diaphragm until the diaphragm leaves are just outside
of the field of view.
Conversion and Use with Transmitted Light45
Care of the Microscope
Leica DM750 M User Manual
Care of the Microscope46
General Maintenance
General
Always carry the microscope using two
hands. There is a handle on the back of
the microscope and an undercut in the front for
this purpose.
OO
•
Leica DM750 M User Manual
The cord wrap allows you to wrap the cord
in such a way that only the length you need
is extended.
OO
Keep all optical components clean. Cleanliness is important for maintaining good
optical performance.
OO
The microscope should always be covered
with the plastic dust cover (provided with
the instrument) when it is not in use.
OO
If any optical surface becomes coated with
dust or dirt, clean the surface by blowing it
off with a syringe or brushing it off with a
camel hair brush before attempting to wipe
the surface clean.
OO
Optical surfaces should be cleaned with a
lint-free cloth, lens tissue, or cotton swab
moistened with a commercially available
glass cleaner.
OO
It is very important to avoid the excessive
use of solvents, so use them sparingly. The
lint-free cloth, lens tissue or cotton swab
should be moistened with solvent, but
not be wet enough for the solvent to seep
around the lens.
Care of the Microscope47
General Maintenance (Continued)
OO
No part of the microscope is quite so
vulnerable to collecting dirt, dust, and oil
as the front lens of the objective. Whenever you encounter lack of contrast, cloudiness or poor definition, carefully check the
condition of the front lens with a magnifier.
OO
Cleaning 40× and 100× objectives requires
more care. Note: To achieve the high degree
of flatness obtained with higher magnification objectives, the objective has a small
concave front lens of fairly short radius or
curvature. The surface of this front lens can
be readily cleaned with a toothpick covered
with a cotton tip, or with a small cotton
swab. Moisten the cotton with commercially available glass cleaner. Wipe the
front lens lightly without applying undue
force or scrubbing action. Make sure that
the cotton tip contacts the concave lens
surface. Check the objective with a magnifier after cleaning.
Leica DM750 M User Manual
OO
If you need to remove the microscope’s
viewing body, be careful not to accidentally
touch the outer lens surface (located on
the underside of the body). Fingerprints on
this surface will reduce image clarity. This
lens can be cleaned in the same manner as
objectives and eyepieces.
Illumination
OO The Leica DM750 M uses LED illumination.
Therefore, no lamp changing is required for
the life of the microscope.
Care of the Microscope48
10/2019 · 13IDE16060EN_2 · © 2019 by Leica Microsystems GmbH.
Subject to modifications. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.
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