3M Petrifilm™ Aqua Yeast and Mold Count Plates Operating instructions

3M Petrifilm™ Aqua Yeast and Mold Count Plates Operating instructions
Interpretation
Guide
The 3M™ Petrifilm™ Aqua Yeast and Mold
Count Plate is a sample-ready culture medium
system, which contains nutrients supplemented
with antibiotics, a cold-water-soluble gelling
agent, and an indicator that facilitates colony
enumeration. 3M Petrifilm Aqua Yeast and Mold
Count Plates are used for the enumeration of
yeast and mold in the bottled water industry.
AQYM
Aqua Yeast and Mold Count Plate
1
Figure 1
Figure 2
Yeast and mold count: 0
Yeast and mold count: 10
3M Petrifilm Aqua Yeast and Mold Count Plate with no colonies.
Mold colonies are large with a dark center and diffuse edge.
Figure 3
Figure 4
Yeast and mold count: 10
Yeast and mold count: 31
Note two small, faint colonies.
Count colonies partially or totally off of the filter.
Aqua Yeast and Mold Count Plate
2
Figure 5
Yeast and mold count: 51
Estimate colony count when molds merge; darker centers
can help enumerate colonies. Count colonies partially or
totally off of the filter.
Reminders for Use
Storage
Hydration Procedure
≤8°C
1
Store unopened pouches at ≤8°C (≤46°F).
Use before expiration date on package. Just
prior to use, allow unopened pouches to
come to room temperature before opening.
2
To seal opened pouches, fold end over and
apply adhesive tape. Do not refrigerate
opened pouches. Use 3M Petrifilm Aqua
Yeast and Mold Count Plates within one
month after opening.
4
Place filter in the center of the plate.
5
With the pipette perpendicular to the
3M Petrifilm Aqua Yeast and Mold
Count Plate, place 1 mL of hydration
diluent onto the center of the filter.
Appropriate sterile diluents include
distilled water, deionized (DI) water and
reverse osmosis (RO) water.
3
6
Following standard procedures for water
analysis, membrane filter water sample
using a 47 mm, 0.45 micron pore size
Mixed Cellulose Ester (MCE) filter.
Carefully roll top film down onto the filter.
3
Incubation
7
10
Lightly apply pressure to the 3M™ Petrifilm™
Yeast and Mold Spreader to ensure uniform
contact of the filter with the gel and to
eliminate any air bubbles.
8
Interpretation
Incubate 3M Petrifilm Aqua Yeast and Mold
Count Plates in a horizontal position, clear
side up, in stacks on no more than 20 plates
at 20–25°C for 3–5 days.
9
3M Petrifilm Aqua Yeast and Mold Count
Plates can be counted on a standard colony
counter or other illuminated magnifier.
Colonies may be isolated for further
identification. Lift top film and pick the
colony from the gel.
3M Food Safety offers a full line of
products to accomplish a variety of
your microbial testing needs. For
more product information, visit us
at 3M.com/foodsafety/Petrifilm or
call 1-800-328-6553.
User’s Responsibilities: User’s Responsibilities: 3M Petrifilm Plate performance has not been
evaluated with all combinations of microbial flora, incubation conditions and food matrices. It is the
user’s responsibility to determine that any test methods are results meet the user’s requirements.
Should re-printing of this Interpretation Guide be necessary, user’s print setting may impact picture
and color quality.
3M Food Safety
3M Center, Building 275-5W-05
St. Paul, MN 55144-1000 USA
1-800-328-6553
3M.com/foodsafety
3M Canada
Post Office Box 5757
London, Ontario N6A 4T1
Canada
1-800-364-3577
Aqua Yeast and Mold Count Plate
For detailed CAUTIONS, DISCLAIMER OF WARRANTIES/LIMITED REMEDY
and LIMITATION OF 3M LIABILITY, STORAGE AND DISPOSAL information
and INSTRUCTIONS FOR USE, see Product’s package insert.
3M and Petrifilm are trademarks of 3M. Used under license in Canada.
Please recycle. Printed in USA. © 3M 2017. All rights reserved.
70-2009-9450-0 (Rev-1217).
4
Was this manual useful for you? yes no
Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Download PDF

advertisement