DiaSorin N-tact PTH SP Parathyroid Hormone Assay Kit Instruction Manual

Below you will find brief information for Parathyroid Hormone Assay Kit N-tact PTH SP. This kit is used for the quantitative determination of biologically active intact human parathyroid hormone (hPTH 1-84) in serum or plasma by immunoradiometric assay. The assay utilizes 2 different polyclonal antibodies that are specific for 2 different regions of the PTH molecule. The first antibody, specific for PTH 39-84, is bound to a solid phase (polystyrene beads). The second antibody is specific for PTH 1-34 and is labeled with iodine-125. Samples are incubated simultaneously with both antibodies. Intact PTH 1-84 contains both the 1-34 and the 39-84 amino acid sequences and is the only form of PTH that will be bound by both the antibody on the bead and the antibody labeled with iodine-125.

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Parathyroid Hormone Assay Kit N-tact PTH SP Instruction Manual | Manualzz

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Key features

  • Quantitative determination of hPTH 1-84
  • Immunoradiometric assay (IRMA)
  • Utilizes 2 different polyclonal antibodies
  • Specific for 2 regions of the PTH molecule
  • Solid phase (polystyrene beads)
  • Iodine-125 labeled antibody
  • Simultaneous incubation with both antibodies

Frequently asked questions

This assay is used to quantitatively determine the concentration of biologically active intact human parathyroid hormone (hPTH 1-84) in serum or plasma.

The assay utilizes 2 different polyclonal antibodies that are specific for 2 different regions of the PTH molecule.

The first antibody, specific for PTH 39-84, is bound to a solid phase (polystyrene beads). The second antibody is specific for PTH 1-34 and is labeled with iodine-125. Samples are incubated simultaneously with both antibodies.
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