CELL-DYN 3200 Service Manual

• 1 - Introduction
• 2 Troubleshooting
• 3 - Parts Lists
• 4 - Removal & Replacement
• 5 - Verification Procedures
CELL-DYN ® 3200 SYSTEM
SERVICE MANUAL
Part Number 9140300
4#0#,QWURGXFWLRQ • Proprietary Information
4#0#,QWURGXFWLRQ
Table of Contents
1.1 Proprietary Information . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 Revision Log. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.3 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Service Manual Section Descriptions . . . . . . . . . . . . . 3
Section 1: Introduction & General Data . . . . . . . . . . . . 3
Section 2: Troubleshooting . . . . . . . . . . . . . . . . . . . . . . 3
Section 3: Parts Lists . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Section 4: Removal & Replacement . . . . . . . . . . . . . . . 3
Section 5: Verification Procedures . . . . . . . . . . . . . . . . 3
Section 6: Preventive Maintenance & Total Call. . . . . . 3
1.4 CELL-DYN 3200 Operator’s Manual . . . . . . . . . . . . . . 3
1.5 Warranty Information. . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.6 System Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
The CELL-DYN 3200 Hematology System . . . . . . . . . . 4
System External Components . . . . . . . . . . . . . . . . . . . 4
System Internal Components . . . . . . . . . . . . . . . . . . . . 5
1.7 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Signal Words and Graphic Warning Conventions . . . 5
Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Graphic Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Biosafety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.8 CELL-DYN 3200 Hematology Parameters . . . . . . . . . . 9
1.9 System Specifications . . . . . . . . . . . . . . . . . . . . . . . . . 10
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
1.1 Proprietary Information
This CELL- DYN 3200 System Service Manual was developed
solely for use with Abbott Laboratories equipment as specified
in the operating instructions. The Manual is copyrighted by
Abbott Laboratories. No part of this document may be
reproduced, stored, or transmitted in any form or by any means
electronic, mechanical, photocopied, recorded, or otherwise
without the prior written permission of Abbott Laboratories.
This CELL- DYN 3200 System Service Manual is published by
Abbott Diagnostics, a Division of Abbott Laboratories, Abbott
Park, IL 60064-3500, U. S. A. Please direct all inquiries
concerning information in this manual to the foregoing address.
CELL- DYN is a registered trademark of Abbott Laboratories.
Vacutainer is a registered trademark of Becton-Dickinson and
Company.
This service manual was developed to be used by trained
Abbott Laboratories Field Service Personnel. The revision
status of this manual is the responsibility of the manual holder.
In no event shall Abbott Laboratories or its subsidiaries be
liable for any damages incurred in connection with or arising
from the use of this manual by persons not fully trained by
Abbott Laboratories.
 1998 by Abbott Laboratories. All rights reserved.
Part Number 9140300 − August 1998
1-1
4#0#,QWURGXFWLRQ • 1.2 Revision Log
1.2 Revision Log
The Revision Status of this manual is indicated below. Be sure
that the manual contains the latest revision number of all
pages.
Table 1-1
Revision Log
Revision
Date
Pages Revised/Added
TSBs Incorporated
ISAs Incorporated
A
August
Complete Revision
109 - 001A, 003
109 - 003, 004, 005, 006
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
1-2
4#0#,QWURGXFWLRQ • 1.3 About This Manual
1.3 About This Manual
Section 4: Removal & Replacement
Introduction
This section contains the procedures to be used by the FSR for
the removal and replacement of subassemblies and
components. The procedures are presented in a paragraph
format and are accompanied by illustrations for added clarity.
This Service Manual contains the information necessary to
align, troubleshoot, repair, and maintain the CELL-DYN 3200
Automated Hematology System.
Service Manual Section Descriptions
The manual is composed of five sections. The following are
descriptions of the contents of each of the sections.
Section 1: Introduction
This section contains an introduction to the system, information
on the use of the manual, warranty information, safety
considerations, hematology parameters, and system
specifications.
Section 5: Verification Procedures
This section contains procedures that enable the FSR to verify
the integrity of various instrument functions. These procedures
may be used to facilitate troubleshooting, or be dictated by
Troubleshooting and Removal and Replacement.
This section also contains procedures such as adjustments,
alignments, and calibrations used to ensure proper instrument
performance. These procedures may be performed on a routine
maintenance basis, or be dictated by Removal and
Replacement.
1.4 CELL-DYN 3200 Operator’s Manual
Section 2: Troubleshooting
This section contains procedures to be used by the FSR in
identifying and isolating problems. The procedures are
presented in a flowchart format and are accompanied by
illustrations for added clarity. A detailed explanation of the use
of the flowcharts is given at the beginning of the section.
Operating instructions and routine maintenance procedures for
the CELL-DYN 3200 are included in the Operator’s Manual
provided with each system. It is essential that the service
engineer read and understand the Operator’s Manual, and be
able to correctly perform all routine operating functions before
attempting to troubleshoot and repair the system.
Section 3: Parts Lists
This section contains parts lists and graphics showing the
locations of FRUs. This section is used by the FSR in
conjunction with Troubleshooting and Removal & Replacement.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
1-3
4#0#,QWURGXFWLRQ • 1.5 Warranty Information
1.5 Warranty Information
System External Components
Components comprising the CELL-DYN 3200 System have
been designed by Abbott Laboratories for optimal performance.
Substitution of reagents, accessories, or system components
may adversely affect performance and may invalidate any
warranty agreements.
The following is a list of the CELL-DYN 3200 external
components and conditions:
•
Operator Technique (Maintenance)
•
System Reagents
•
– Isotonic Diluent
– Diluent/Sheath
– Hemoglobin Lyse
– WBC Lyse
System Controls & Calibrators
•
– CELL-DYN 3200 Whole Blood Control
– OPTI-CAL
– Latex Beads
Whole Blood Specimens
•
System Environmental Integrity (Temperature, RFI, ESD)
•
Power Line Integrity
Abbott Laboratories cannot accept responsibility for any clinical
results produced by use of reagents, calibrators, controls,
equipment, or supplies either not manufactured by or not
approved by Abbott Laboratories.
1.6 System Introduction
Each CELL-DYN 3200 System may be comprised of three or
four major hardware components: Analyzer, Video Display,
Keyboard, and optional Sample Loader.
The CELL-DYN 3200 Hematology System
When approaching a hematology system from a service
perspective, the word “system” must be emphasized because
there are both “internal components” (system hardware and
software) and “external components” (reagents, controls, etc.)
that comprise the complete system. The Service
Representative must realize that both of these types of
components can adversely affect overall system performance.
Therefore, it follows that all external components and
conditions such as reagents, environment, integrity of samples
and controls be checked and verified as correct before
performing service on the internal components. In the
investigation of any non-specific or data related complaint, the
system hardware and software should be the last components
of the system to be suspected as the cause of the problem.
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
1-4
4#0#,QWURGXFWLRQ • 1.7 Safety
System Internal Components
The following is a list of the CELL-DYN 3200 internal
components:
•
CELL-DYN 3200 System Components
–
–
–
–
–
–
–
–
–
Flow Panel
Optics Bench
Power Supplies
Measurement Electronics
Computer Electronics
A/D & D/A Electronics
Air Supply
Personal Computer Module
Tube Manipulation Hardware
Signal Words and Graphic Warning
Conventions
Signal Words
'$1*(5=##Denotes an immediate hazard which, if not
avoided, could result in serious injury or death. This signal word
represents the highest level of any hazardous situation.
:$51,1*=##Denotes a hazard which, if not avoided, could
result in moderate to serious injury.
&$87,21=##Denotes potential hazards that could result in
minor injury. Also, used for conditions or activities that could
threaten equipment performance.
Graphic Conventions
1.7 Safety
Operation, maintenance, and servicing of hematology systems
may expose individuals to potential safety and health hazards.
All work must be performed in accordance with procedures
described in the Abbott Operator’s and Service Manuals, or as
directed by an Abbott Technical Support Representative.
Warnings are inserted in this manual to alert personnel to
potential hazards. The standard warning conventions including
signal words (e.g. Caution) and symbols are described below.
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
The general hazard icon identifies an activity or area
that may present a hazard to personnel or equipment.
The electrical hazard icon alerts personnel to the
possibility of electrical shock if procedural or
engineering controls are not observed.
Part Number 9140300 − August 1998
1-5
4#0#,QWURGXFWLRQ • 1.7 Safety
The biohazard icon identifies an activity or area where
personnel may be exposed to infectious substances.
The laser hazard icon identifies an activity or area
where personnel will be exposed to an eye hazard if
procedural or engineering controls are not observed.
Clean-up spills of potentially infectious materials in accordance
with established biosafety practices. A generally accepted
procedure for cleaning such spills is to absorb the spill with
toweling or other absorbent material, wipe the area with a
detergent solution, and then wipe the area with an appropriate
tuberculocidal disinfectant such as 10% chlorine bleach
solution.
Prior to service or maintenance, the instrument should be
decontaminated in accordance with the following:
a. Remove and dispose of contaminated disposables in a
regulated medical waste container.
The electrostatic discharge icon identifies an activity or
area where personnel must wear a ground strap while
servicing the system.
Biosafety
:$51,1*=#327(17,$/#%,2+$=$5' Consider
all clinical specimens, reagent controls, surfaces, or
components that contain or have contacted human
blood or serum as potentially infectious. Wear gloves,
lab coats and safety glasses, and follow other
biosafety practices as specified in the OSHA
Bloodborne Pathogen Rule (29CFR Part 1910.1030)
or other equivalent biosafety procedures.
b. Clean and decontaminate the exterior of the
instrument using a detergent solution followed by a
10% chlorine bleach solution or other tuberculocidal
disinfectant. Flush the fluid pathway with chlorine
bleach solution as specified in the Operator’s Manual.
Dispose of waste in accordance with local, state and federal
regulations. Probes, needles, broken glass, slides, and other
sharps that are contaminated with potentially infectious
substances should be collected in a “sharps” container for
disposal as regulated medical waste. Contaminated gloves,
wipes, and other disposables should be placed in a standard
medical waste container.
:$51,1*=#327(17,$/#%,2+$=$5'#The
aspiration needle/probe is sharp and potentially
contaminated with infectious materials. Avoid contact
with the tip of the needle/probe.
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
1-6
4#0#,QWURGXFWLRQ • 1.7 Safety
Electrical Safety
:$51,1*= (/(&75,&$/#+$=$5' When the
instrument is powered and protective covers are
removed, there are exposed electrical systems that
could startle or seriously injure personnel upon
contact. Use appropriate safety precautions to prevent
body and/or tool contact with live electrical
components, especially power supplies.
Laser Safety
DANGER: LASER LIGHT Avoid direct exposure to
beam. Do not look directly into the laser beam or any
reflection of the beam from a mirror-like surface.
When the access door, instrument top cover, or other
inner protective cover is removed, (Helium-Neon)
laser power up to 10 mW continuous wave at 632.8
nm in a beam with 1 mR divergence could be
accessible in the interior of the optics bench. This
amount of energy, with insignificant attenuation with
distance, is sufficient to cause eye damage.
The Class 1 Laser Product Label (Abbott PN 9230702A) is
shown below (Figure 1-1). The label consists of black lettering
against a white background. The label is located on the
backside of the instrument and positioned at a clearly visible
location.
The inner protective cover laser warning labels must not be
removed and are to remain legible. The protective housing
label (Abbott PN 9230701D) is shown in Figure 1-2. The label
consists of black lettering against a yellow background and
appears on the protective cover within the optics bench and on
the flow panel (see Figure 1-3 and Figure 1-4).
32109
Figure 1-1
Laser Product Label (PN 9230702A)
&$87,21: Use of controls or adjustments or
performance of procedures other than those specified
herein may result in hazardous laser light exposure. If
the instrument is used or modified in a manner not
specified by the manufacturer, the protection provided
by the instrument may be impaired.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
1-7
4#0#,QWURGXFWLRQ • 1.7 Safety
.CUGT
9CTPKPI
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Figure 1-2
Laser Warning Label (PN9230702D)
Figure 1-3
Top Laser Warning Label Location
For beam alignment and other open beam configurations,
follow instructions provided by the Service Manual, Advisories,
and Bulletins regarding the requirement for using laser safety
eyewear. If required, assure laser eyewear are not damaged
and have an optical density of 1- 2 at a wavelength of 632.8
nm. During open beam configurations, assure that the beam is
confined to the laser bench area and only personnel with proper
eye protection are present.
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
1-8
4#0#,QWURGXFWLRQ • 1.8 CELL-DYN 3200 Hematology Parameters
.CUGT
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1.8 CELL-DYN 3200 Hematology
Parameters
The following tables list the CELL-DYN 3200 Hematology
Parameters and their abbreviations.
Table 1-2
Red Cell Parameters
Full Name
Red Blood Cell Concentration
RBC
Hemoglobin Concentration
HGB
Hematocrit
HCT
Mean Cell Volume
MCV
Mean Cell Hemoglobin
MCH
Mean Cell Hemoglobin Concentration
Red Cell Distribution Width
Table 1-3
Figure 1-4
Front Laser Warning Label Location
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Abbreviation
MCHC
RDW
White Cell Parameters
Full Name
Abbreviation
White Blood Cell Concentration
WBC
Neutrophil Concentration
NEU
Neutrophil Percentage
%N
Lymphocyte Concentration
LYM
Lymphocyte Percentage
%L
Monocyte Concentration
MONO
Monocyte Percentage
%M
Eosinophil Concentration
EOS
Part Number 9140300 − August 1998
1-9
4#0#,QWURGXFWLRQ • 1.9 System Specifications
Table 1-3
1.9 System Specifications
White Cell Parameters (Continued)
Full Name
Abbreviation
Eosinophil Percentage
%E
Basophil Concentration
BASO
Basophil Percentage
Table 1-4
Abbreviation
Platelet Concentration
PLT
Mean Platelet Volume
MPV
Platelet-crit
PCT*
Platelet Distribution Width
PDW*
* Clinical significance has not been established for these
parameters. Therefore they are not reportable in the US.
They are provided for laboratory use only.
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Table 1-5
Physical Specifications (Unpackaged)
%B
Platelet Parameters
Full Name
The following tables contain the specification for the CELL-DYN
3200 System.
CS Model
SL Model
Graphics
Printer
Height
19.0” (48.2 cm)
19.0” (48.2 cm)
7.2” (18.3 cm)
Width
32.8” (83.3 cm)
32.8” (83.3 cm)
16.1” (40.9 cm)
Depth
23.3” (59.2 cm)
29.3” (74.4 cm)
9.9” (25.1 cm)
Weight
214 lb (97.3 kg) 232 lb (105.5 kg)
Table 1-6
9.9 lb (4.5 kg)
Physical Specifications (Packaged)
CS Model
SL Model
Graphics
Printer
Height
38” (96.5 cm)
38” (96.5 cm)
12” (30.5 cm)
Width
34” (86.3 cm)
34” (86.3 cm)
19.5“ (49.5 cm)
Depth
31” (78.7 cm)
31” (78.7 cm)
14.8” (37.6 cm)
Weight
255 lb (116 kg)
273 lb (124 kg)
16.4 lb (7.4 kg)
Part Number 9140300 − August 1998
1 - 10
4#0#,QWURGXFWLRQ • 1.9 System Specifications
Table 1-7
Module
Analyzer
Analyzer &
Sample
Loader
Monitor
Printer
Analyzer Input Requirements
Table 1-8
Voltage
Frequency
Max
Current
110 VAC + 10 %
50/60 ± 3 Hz
6 amps
120 VAC + 10 %
50/60 ± 3 Hz
6 amps
220 VAC + 10 %
50/60 ± 3 Hz
3 amps
240 VAC + 10 %
50/60 ± 3 Hz
3 amps
110 VAC + 10 %
50/60 ± 3 Hz
120 VAC + 10 %
Performance Specifications
Background Precision Correlation
Linear
Range
WOC
≤ 0.10K/µL
≤ 2.7%
≥ 0.99
0.0-250 K/µL
NOC
≤0.10 K/µL
2.7%
≥ 0.99
0.0-250 K/µL
RBC
≤ 0.20 M/µL
≤ 1.5%
≥ 0.98
0.0-8.0 M/µL
6 amps
HGB
≤ 0.1g/dl
≤ 1.0%
≥ 0.98
0.0-25.0g/dL
50/60 ± 3 Hz
6 amps
MCV
N/A
≤ 1.0%
≥ 0.98
35-180 fL
220 VAC + 10 %
50/60 ± 3 Hz
3 amps
50/60 ± 3 Hz
RDW
N/A
≤ 5.0%
≥ 0.91
N/A
240 VAC + 10 %
3 amps
100 - 240 VAC
50/60
1.3 amps
PLT
≤5.0K/µL
≤ 4.0%
≥ 0.98
0.0 - 1750
K/µL
50/60
0.5 amps
MPV
N/A
≤ 5.0%
≥ 0.72
2.0-18.0 fL
120 VAC ± 10%
220 - 260 VAC
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
1 - 11
4#0#,QWURGXFWLRQ • 1.9 System Specifications
Table 1-9
Operational Specifications
Operating Environment
Specimen Tube Dimensions
Diameter
13 mm
75 mm
Temperature
15o - 30o C (59o - 86o F)
Length
Relative Humidity
20% to 80%, RHNC
Minimum Specimen Volumes
Closed and Sample Loader Mode
Complete Cycle Times
Startup (From Power Off)
Startup (From Standby)
Count Cycle, Patient
Count Cycle + FrgWBC
Count Cycle + ResRBC
Shutdown (To Standby)
Shutdown (To Power Off)
~ 7 minutes
~ 5 minutes
~ 52 seconds
~ 100 seconds
~ 100 seconds
~ 130 seconds
~ 130 seconds
Open Mode
≥ 1 mL
500 µL
Batch Size
1 to 50 tubes per batch
Bar Code Formats
Interleaved 2-of-5 code
Codabar
Code 39
Throughput
Code 128 (check digit only)
CS Model
Bar Code Specifications
Open
80 Samples/Hour
•
Printed on good quality label stock
Closed
68 Samples/Hour
•
0.25-inch quiet zone on each end
•
0.01-inch minimum narrow bar width
SL Model
Open
80 Samples/Hour
•
0.5-inch minimum bar length
Closed
68 Samples/Hour
•
2-inch maximum label length
•
1.25-inch maximum label width
Whole Blood Aspiration Volume
Open Mode
125 µL
Closed Mode
250 µL
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
1 - 12
5#7URXEOHVKRRWLQJ • Table of Contents
TOC
5##7URXEOHVKRRWLQJ
Table of Contents
2.1
2.2
2.3
Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Troubleshooting Approach . . . . . . . . . . . . . 2-2
Error Messages . . . . . . . . . . . . . . . . . . . . . . . 2-3
IP-1
IP-2
IP-3
IP-4
IP-5
IP-6
IP-7
IP-8
IP-9
IP-10
IP-11
IP-12
IP-13
IP-14
IP-15
IP-16
IP-17
IP-18
IP-19
IP-20
IP-21
IP-22
IP-23
IP-24
Fluid Leaks, Open Mode Probe or Wash Block . . . 2-4
Fluid Leaks, Closed Mode Needle or Wash Block . 2-6
Incomplete Aspiration . . . . . . . . . . . . . . . . . . . . . . . 2-8
Flow Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
RBC/HGB Diluent Overpressure . . . . . . . . . . . . . 2-13
Optical Parameter Count Problems . . . . . . . . . . . 2-15
Reagent Empty . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-26
Optical Backgrounds Too High . . . . . . . . . . . . . . . 2-31
Histogram Problems . . . . . . . . . . . . . . . . . . . . . . . 2-32
Controls Out . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-33
Vacuum Accumulator #1 Wet . . . . . . . . . . . . . . . . 2-35
Vacuum Accumulator #2 Wet . . . . . . . . . . . . . . . . 2-37
HGB Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-39
X-B Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-41
Optical Parameters Imprecision . . . . . . . . . . . . . . 2-44
HGB Imprecision . . . . . . . . . . . . . . . . . . . . . . . . . 2-47
Primary Vacuum/Pressure Isolation, . . . . . . . . . . 2-48
Pressure Leak Isolation . . . . . . . . . . . . . . . . . . . . 2-57
Vacuum Leak Isolation . . . . . . . . . . . . . . . . . . . . . 2-64
Shear Valve Position Fault . . . . . . . . . . . . . . . . . . 2-70
Flags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-72
External Waste Full . . . . . . . . . . . . . . . . . . . . . . . 2-74
Blank Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-76
HSSL Failures/Message Reception Time-Out . . . 2-77
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Hard Drive Errors . . . . . . . . . . . . . . . . . . . . . . . . . 2-78
Printer Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-79
Sample Loader & Aspiration Tower
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . 2-81
Appendix 2A -Cable Connection Diagram . . . . . . . . . . . . 2A-1
Appendix 2B -System Block Diagrams . . . . . . . . . . . . . . 2B-1
Appendix 2C -System Flow Diagrams . . . . . . . . . . . . . . . 2C-1
IP-25
IP-26
IP-27
Part Number 9140300-August 1998
2-1
5#0#7URXEOHVKRRWLQJ • 2.1 Summary
2.1 Summary
c. To refer to the appropriate IP or VP, click on the red
link.
This chapter contains the following information for CELL-DYN
3200:
d. Begin at the START box and follow the directions in the
IP.
•
Error Messages
•
Isolation Procedures (IPs)
•
Block Diagrams
•
Normal Scatter/Histograms
•
Service Commands
e. If a part is adjusted or replaced, go to Section 5.2,
Verification Matrix.
f.
Find the part in left column.
g. Perform VPs in order given (i.e., 1, 2, 3, 4 etc.)
2. Perform Total Call.
2.2 Troubleshooting Approach
Troubleshooting is required whenever an Observed Error or a
Displayed Error occurs. Displayed Errors can be viewed on the
Bulletin Line or status box of the Data Station screen. (The
Bulletin Line is down about 3/4 way on the screen.) Detailed
information can be found in the fault report screen of the
diagnostics submenu.
When a malfunction occurs, follow these recommended
procedures:
1. Use this Troubleshooting Guide as follows:
a. If possible, begin by verifying the reported error.
Perform the exact procedure that produced the error
condition.
b. When an error occurs, either observed or displayed,
look up the error in:
„
„
the Error Messages table or
IP 27, Sample Loader & Aspiration Tower Error
Messages table
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 - August 1998
2-2
5#0#7URXEOHVKRRWLQJ • 2.3 Error Messages
2.3 Error Messages
Error
Error
Corrective Action
Corrective Action
Invalid Tube Height
IP-27
Leaks, Pressure
IP-18
Leaks, Vacuum
IP-19
Background, HGB Too High
IP-13
Background, RBC/ PLT Too High
IP-8
Background, WBC Too High
IP-8
Load Zone Empty Detector
Malfunction
IP-27
Bar Code Reader Failure
IP-27
MCH Errors
IP-14
C:\ Hard Drive Errors
IP-25
MCHC Errors
IP-14
Controls Out
IP-10
MCV Errors
IP-14
Diluent/Sheath Empty
IP-7
Message Reception Timeout
IP-24
Erratic Results, HGB
IP-13
Mix Head Not At Top Position
IP-27
Mix Head Stuck At Top Position
IP-27
Mix Head Stuck In Angle Position
IP-27
Erratic Results, Optical
IP-6 Ensure Blood Sam
ple is Fresh. Differential
count is not reliable
before 30 minutes or after
12 hours after sample is
drawn.
Mix Head Stuck In Vertical
Position
IP-27
Mix Zone Rack Position Error
IP-27
External Waste Full
IP-22
No Tube Present
IP-27
Flags
IP-21
Overpressure, Diluent Syringe
IP-5
Flow Errors
IP-4
Precision Problems
HGB IP-16, OPT IP-15
Fluid Leaks, Closed Mode Probe
IP-2
Pressure Errors
IP-17
Fluid Leaks, Open Mode Probe
IP-1
Printer Errors
IP-26
HCT Errors
IP-14
Probe Stuck At Home Position
IP-27
HGB Errors
IP-13
IP-27
Histogram Errors
IP-9
Probe Unable To Reach Home
Position
HSSL Com Failures
IP-24
Rack Bar Code Read Failure
IP-27
Incomplete Aspiration
IP-3
Shear Valve Position Fault
IP-20
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 - August 1998
2-3
5#0#7URXEOHVKRRWLQJ • 2.3 Error Messages
Error
Corrective Action
Tube Dropped During Mixing
IP-27
Tube Lost Moving From Position
3 To 4
IP-27
Tube Position Bar Code Read
Failure
IP-27
Tube Stuck In Position 3
IP-27
Tube Stuck In Position 4
IP-27
Unexpected Tube In Position 4
After Rack Advance
IP-27
Unload Area Hardware
Malfunction
IP-27
Unload Zone Full Detector
Malfunction
IP-27
Unload Zone Nearly Full Detector
Malfunction
IP-27
Vacuum Accumulator 1 Wet
IP-11
Vacuum Accumulator 2 Wet
IP-12
Vacuum Errors
IP-17
WBC Imprecision
IP-15 Ensure Blood Sample is Fresh. Differential
count is not reliable
before 30 minutes or after
12 hours after sample is
drawn.
WBC/HGB Lyse Empty
IP-7
X-B Errors
IP-14
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 - August 1998
2 - 3A
5#7URXEOHVKRRWLQJ • IP-1 Fluid Leaks, Open Mode Probe or Wash Block
IP-1 Fluid Leaks, Open Mode Probe or Wash Block (Page 1 of 2)
Start
YES
- Clamp tubing at the top of
Valve 11
- Clamp tubing between
Ultrasonic sensor and
Shear valve
NO
Probe Leaking
In Ready Mode
Leaking
Stops
?
?
Leaking
Stops
YES
NO
?
YES
Wash Block Leaking
In Ready Mode
- Verify connections on
Ultrasonic Sensor
- Verify and tighten fittings
on Y-Valve
- Verify connection on top
of Probe
- Replace Y-Valve (PL-8)
NO
?
YES
1
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
2
- Verify pinch tubing in
Valve 11
- Verify and replace
Valve 11 (PL-4.1)
NO
- Verify Shear valve
fittings
- Clean Shear Valve
(VP-48)
- Replace Shear Valve
(PL-9.1)
Part Number 9140300-August 1998
2-4
5#7URXEOHVKRRWLQJ • IP-1 Fluid Leaks, Open Mode Probe or Wash Block
IP-1 Fluid Leaks, Open Mode Probe or Wash Block (Page 2 of 2)
1
2
- Verify pinch tubing in
Valve 15
- Verify and replace Valve
15 (PL-4.1)
- Run a several Count
Cycles while observing the
bottom of the Wash Block
YES
- Ensure fittings on Wash
block are not leaking
NO
Liquid
Leaking
?
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
- Ensure Vacuum #1 and Pressure #3
are within specifications (VP-16)
- Ensure bottom fitting on Wash Block
is not restricted
- Ensure tubing between Wash Block
and Valve 14 is not restricted
- Verify operation of Valve 14 and
replace if necessary (VP-10), (PL-4.1)
- Replace pinch tubing in Valve 14
(TM-1)
- Ensure tubing between Valve 14 and
WC #4 is not restricted
- Verify operation of right Vent Trap
Check Valve and replace if necessary
(TM-1)
- Ensure tubng between WC #4 and
Valve 31 is not restricted
- Verify operation of Valve 31 and
replace if necessary (VP-10), (PL-4.1)
- Replace pinch tubing in Valve 31
(TM-1)
Part Number 9140300 − August 1998
2-5
5#7URXEOHVKRRWLQJ • IP-2 Fluid Leaks, Closed Mode Needle or Wash Block
IP-2 Fluid Leaks, Closed Mode Needle or Wash Block (Page 1 of 2)
Start
- Clamp tubing at the top of
Valve 11
- Clamp tubing between
Ultrasonic sensor and
Shear valve
Leave tubing connected, and
remove Needle from Wash Block
NO
YES
Leaking
Stops
Probe Leaking
In Ready Mode
?
Leaking
Stops
?
?
YES
NO
YES
Wash Block Leaking
In Ready Mode
- Verify connections on
Ultrasonic Sensor
- Verify and tighten fittings
on Y-Valve
- Verify connection on top
of Probe
- Replace Y-Valve (PL-8)
NO
?
YES
1
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
- Verify pinch tubing in
Valve 11
- Verify and replace
Valve 11 (PL-4.1)
NO
- Verify Shear valve
fittings
- Clean Shear Valve
(VP-48)
- Replace Shear Valve
(PL-9.1)
2
Part Number 9140300 − August 1998
2-6
5#7URXEOHVKRRWLQJ • IP-2 Fluid Leaks, Closed Mode Needle or Wash Block
IP-2 Fluid Leaks, Closed Mode Needle or Wash Block (Page 2 of 2)
1
2
- Verify pinch tubing in
Valve 34
- Verify and replace Valve
34 (PL-4.1)
- Run a several Count
Cycles while observing the
bottom of the Wash Block
YES
- Ensure fittings on Wash
block are not leaking
NO
Liquid
Leaking
?
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
- Ensure Vacuum #1 and Pressure #3 are
within specifications (VP-16)
- Ensure bottom fitting on Wash Block is not
restricted
- Ensure tubing between Wash Block and
Valve 37 is not restricted
- Verify operation of Valve 37 and replace if
necessary (VP-10), (PL-4.1)
- Replace pinch tubing in Valve 37 (TM-1)
- Ensure tubing between Valve 37 and WC #4
is not restricted
- Verify operation of right Vent Trap Check
Valve and replace if necessary (TM-1)
- Ensure tubng between WC #4 and Valve 31 is
not restricted
- Verify operation of Valve 31 and replace if
necessary (VP-10), (PL-4.1)
- Replace pinch tubing in Valve 31 (TM-1)
Part Number 9140300 − August 1998
2-7
5#7URXEOHVKRRWLQJ • IP-3 Incomplete Aspiration
IP-3 Incomplete Aspiration (Page 1 of 3)
Start
NO
- Verify Y-Valve is rotating
properly (VP-7) .
- Aspirate Normal
Control in both open
and closed modes.
YES
- Clean Shear Valve (VP-48) .
Rotating
Properly
Problem
Corrected
?
YES
Mode(s)
Affected
?
Open Only
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Both
NO
Closed Only
- Replace Y-Valve Assy (PL-8) .
- Replace MDM (Pl-9.2)
Total Call
?
1
2
4
- Replace SDM/CDM (PL-15) .
Part Number 9140300 − August 1998
2-8
5#7URXEOHVKRRWLQJ • IP-3 Incomplete Aspiration
IP-3 Incomplete Aspiration (Page 2 of 3)
2
1
- Run an open cycle and
time the blood movement
to the Ultrasonic Sensor.
Start when Valve 12
opens and stop when
blood reaches sensor.
YES
< 3.5 Seconds
?
- Run a closed cycle and
time the blood
movement to the
Ultrasonic Sensor. Start
when Valve 12 opens
and stop when blood
reaches sensor.
- Place a blood sample
under Open Probe and
manually open Valve 12
until the blood is just
past the Y-Valve.
- Verify an air gap of
approximately 1 inch
between the diluent and
blood columns.
- Manually place a blood
sample under Closed
Probe.
- From Main Menu, press
„ DIAGNOSTICS
YES
„ MORE
„ TOWER TEST
„ NEEDLE DOWN
- If no air gap is present,
check the Open Probe
plumbing for air leaks.
- Manually open Valve 12
until the blood is just
past the Y-Valve.
< 7.0 Seconds
- Verify an air gap of
approximately 3 inches
between the diluent and
blood columns.
?
NO
NO
- Clean Closed Probe (VP-51).
- Clean Open Probe (VP-50).
- Ensure there are no
restrictions between Open
Probe and Y-Valve.
- Replace Open Probe (PL-8).
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
- Ensure there are no
restrictions between Closed
Probe and Y-Valve.
NO
- Replace Closed Probe (PL-8).
- Verify Valve 38 (VP-10) and
ensure Vent Trap plumbing is
free of restrictions.
Air Gap
Present
?
- Check the Closed
Probe plumbing for air
leaks.
Part Number 9140300 − August 1998
YES
3
2-9
5#7URXEOHVKRRWLQJ • IP-3 Incomplete Aspiration
IP-3 Incomplete Aspiration (Page 3 of 3)
3
4
- INITIALIZE and PRIME
instrument.
- Run a closed cycle and
verify that the blood
column stops just past
Sample Sensor #2.
- Run an open cycle and
time the blood
movement to the
Ultrasonic Sensor. Start
when Valve 12 opens
and stop when blood
reaches sensor.
- Run an open cycle and
verify that blood column
stops just past
Ultrasonic Sensor. I
blood does not stop:
- If blood does not stop:
- Re-seat Ultrasonic
Sensor Cable.
- Perform Sample Sensor
adjustment (VP-12).
- Replace Ultrasonic
Sensor (PL-8).
- Re-seat sensor cable.
- Replace Ultrasonic
Sensor Board (PL-9.2).
- Replace Sample
Sensor (PL-9.1).
- Replace Sample
Sensor Board (PL-9.2).
YES
< 3.5 Seconds
?
NO
- Verify Vac 2 level (VP-16). Troubleshoot as necessary.
- Ensure there are no restrictions between Y-Valve and
Valve 12
- Verify Valve 12 and tubing (VP-10). Replace as necessary
(PL-4.1), (TM-1).
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 10
5#7URXEOHVKRRWLQJ • IP-4 Flow Errors
IP-4 Flow Errors (Page 1 of 2)
Sample
Staging
Pump
Start
From Main Menu:
YES
- Run a Count Cycle and observe
the Delivery Syringe and WC1.
Verify that when the syringe is
moving up, liquid is dripping into
WC1 at a fast and constant rate
(See Figures).
„ DIAGNOSTICS
„ MORE
„ MOTOR OPERATION
„ EXERCISE MOTORS
WC1
- Type A and press Enter.
- Observe the Delivery Syringe
and verify smooth upward
movement (See Figures).
68
Delivery Syringe
Dripping
OK
?
NO
1
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Smooth
Movement
?
YES
NO
3
2
Part Number 9140300 − August 1998
2 - 11
5#7URXEOHVKRRWLQJ • IP-4 Flow Errors
IP-4 Flow Errors (Page 2 of 2)
1
- Verify level of 9 psi (VP-16)
2
3
- Check Laser for stable
output (VP-46).
- Verify tubing and operation of
Valves 64, 65, 91, and 56 and
replace as necessary (TM-1),
(PL-4.1).
- Remove and clean Delivery
Syringe.
- Replace Delivery Syringe
(PL-7).
- Replace Syringe Driver
Assembly (PL-7).
- Replace MPM (PL-11).
- Perform Optical Flow Cell
Cleaning Procedure (VP-44).
- Replace In-Line Sheath Filter
(PL-4.2).
Output
Stable
YES
?
NO
- Remove, inspect, and clean
Flow Cell Nozzle.
- Replace Flow Cell Assy. (PL-10).
- Replace Laser (PL-10).
- Replace Laser Power Supply
(PL-2.2).
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 12
5#7URXEOHVKRRWLQJ • IP-5 RBC/HGB Diluent Overpressure
IP-5 RBC/HGB Diluent Overpressure (Page 1 of 2)
Start
- Check resistance of
Overpressure Sensor
- Remove, inspect, and clean
Shear Valve (VP-48)
NO
Sensor
Shorted
?
- Verify Shear Valve rotation time
is <1.1 sec
From Main Menu:
„ DIAGNOSTICS
- Ensure diluent paths from
syringe to RBC and HGB
Mixing Chambers are free of
restrictions
- Verify operation of Valves 27
and 26 (VP-10) and replace if
necessary (PL-4.1)
„ MORE
„ MOTOR OPERATION
„ SHEAR VAL DISPENSE
NO
„ SHEAR VAL TIME
„ SHEAR VAL ASPIRATE
„ SHEAR VAL TIME
YES
- Replace Sensor (PL-4.2)
Problem
Corrected
Times
OK
YES
?
Total Call
?
NO
YES
1
2
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 13
5#7URXEOHVKRRWLQJ • IP-5 RBC/HGB Diluent Overpressure
IP-5 RBC/HGB Diluent Overpressure (Page 2 of 2)
2
1
In Ready Mode, try to manually
rotate the front and back of the
Shear Valve.
Shear Valve Sections
Should Not Move
- Lubricate Shear Valve Driver
(VP-42)
If problem persists:
- Replace Shear Valve Driver
(PL-9.1)
If there is movement:
- Replace defective section:
Front (PL-9.1)
Rear (PL-9.1)
If problem persists:
- Replace Shear Valve Driver
(PL-9.1)
Rim Notch
Faces Right
Total Call
32002
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 14
5#7URXEOHVKRRWLQJ • IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 1 of 11)
Note: This procedure assumes that HGB Results are OK in Open and Closed Modes, and there are no hardware or software Error Messages.
It is designed to find and correct gross WBC, NOC, RBC, and PLT problems. For Optical Precision Problems see IP-15.
Start
- Verify proper Reagents and Expiration
Date.
- Perform Background Count Verification
(VP-56).
Backgrounds
OK
NO
Go to (IP-8),
correct and
return here.
?
All ≈ "0"
YES
RBC Only ≈ "0"
3
?
?
NO
NO
RBC & PLT
Only ≈ "0"
YES
PLT Only ≈ "0"
4
YES
YES
6
7
?
?
YES
NO
NO
NO
From Main Menu, press
„ RUN
„ SPECIMEN TYPE
„ FRAGILE WBC
- Run a Normal Control five times and
record the optical values.
WBC Only ≈ "0"
YES
5
NOC Only ≈ "0"
YES
8
?
?
NO
- Compare the results to the Data Sheet.
1
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 15
5#7URXEOHVKRRWLQJ • IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 2 of 11)
1
NOC Only
Low
All Low
YES
9
?
YES
WBC Only
High
12
?
?
NO
NO
YES
15
NO
All High
RBC & PLT
Only Low
YES
YES
NOC Only
High
13
?
NO
16
?
?
10
YES
NO
NO
2
NO
RBC & PLT
Only High
WBC Only
Low
YES
11
YES
14
?
?
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 16
5#7URXEOHVKRRWLQJ • IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 3 of 11)
2
All Erratic
YES
WBC Only
Erratic
17
?
?
NO
NO
RBC & PLT
Only Erratic
?
YES
18
NO
NOC Only
Erratic
YES
YES
19
20
?
NO
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 17
5#7URXEOHVKRRWLQJ • IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 4 of 11)
3
- Verify beam alignment
(VP-20), (VP-21).
- Verify laser power (VP-46).
- Verify samples are being staged to
Optical Flow Cell by Peri-pump.
Troubleshoot as necessary.
YES
- Verify Sheath Reservoir is filling
properly.
- Verify Pressure 2 (VP-16) and
proper Sheath flow to Optical Flow
Cell. Troubleshoot as necessary.
- Replace In-Line Filter (PL-4.2).
- Verify operation of Sample
Injection Syringe (VP-6), (VP-7).
Troubleshoot as necessary.
NO
Power OK
- If not able to get data on
the Extended Count
Screen, replace in order
the Optical Flow Cell,
MAM, SPM, and CPU/
D C M (PL-10), (PL-12),
(PL-13).
?
NO
NO
- Connect new Laser Head to
Laser Power Supply and
verify power (see Warning).
If good, replace Laser Head
(PL-10). If not, replace Laser
Power Supply (PL-2).
Problem
Corrected
?
YES
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RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#WKH#ODVHU#FRQQHFWRU#
ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#PDOH#SLQV1#6KRUW#PDOH#SLQV#
WR#&KDVVLV1
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 18
5#7URXEOHVKRRWLQJ • IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 5 of 11)
4
5
6
- Verify RBC/PLT Sample is being
staged to Optical Flow Cell.
- Verify WBC Sample is being
staged to Optical Flow Cell.
- Verify operation of Valve 54 (VP10) and tubing. Replace as
necessary (PL-4.1), (TM-1).
- Verify operation of Valve 53
and 55 (VP-10) and tubing.
Replace as necessary (PL4.1), (TM-1).
- From Main Menu, press
„ DIAGNOSTICS
„ F12
„ F1
„ SET POINT ENTRY
- Verify RBC/PLT Threshold is
550.
- Reseat Preamp, MAM, SPM,
CPU/DCM Cables.
- Replace MAM (PL-12).
- Replace 10 o Preamp (PL-10).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).
7
- Replace MAM (PL-12).
- Replace MAM (PL-12).
- Replace SPM (PL-10).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).
- Replace CPU/DCM (PL-13).
8
- Verify operation of WBC
Syringe (VP-6), (VP-7).
- Verify NOC Sample is
being staged to Optical
Flow Cell.
- From Main Menu, press
„ DIAGNOSTICS
„ F12
- Verify operation of Valve
41 (VP-10) and tubing.
Replace as necessary
(PL-4.1), (TM-1).
„ F1
„ SET POINT ENTRY
- Verify WBC Threshold is
360.
- From Main Menu, press
- Reseat Preamp, MAM, SPM,
CPU/DCM Cables.
„ DIAGNOSTICS
- Replace MAM (PL-12).
„ F1
o
„ F12
- Replace 0 Preamp (PL-10).
„ SET POINT ENTRY
- Replace SPM (PL-10).
- Verify WBC Threshold is
400.
- Replace CPU/DCM (PL-13).
- Perform NOC Gain
Adjustment (VP-25).
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 19
5#7URXEOHVKRRWLQJ • IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 6 of 11)
9
- Verify laser power (VP-46).
- Clean Laser Bench (VP-43).
-- Verify Sheath Reservoir is filling
properly.
YES
- Verify Pressure 2 (VP-16) and
proper Sheath flow to Optical Flow
Cell. Troubleshoot as necessary.
Power OK
- Replace In-Line Filter (PL-4.2).
- Verify operation of Sample
Injection Syringe (VP-6), (VP-7).
Troubleshoot as necessary.
- Replace Vent Accumulator Filter
(PL-4.2).
NO
Problem
Corrected
?
YES
?
NO
- Clean, debubble, and wet
Optical Flow Cell (VP-44),
(VP-45).
- Verify beam alignment (VP20), (VP-21).
- Replace Optical Flow Cell (PL10).
- Replace MAM (PL-12).
NO
- Replace CPU/DCM (PL-13).
- Connect new Laser Head to
Laser Power Supply and
verify power (See Warning).
If good, replace Laser Head
(PL-10). If not, replace
Laser Power Supply (PL-2).
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'DQJHU
LV#RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#WKH#ODVHU#
FRQQHFWRU#ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#PDOH#SLQV1#
6KRUW#PDOH#SLQV#WR#&KDVVLV1
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 20
5#7URXEOHVKRRWLQJ • IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 7 of 11)
11
10
- Verify RBC/PLT Sample is
being staged to Optical
Flow Cell.
- Verify operation of Valve 54
(VP-10) and tubing.
Replace as necessary (PL4.1), (TM-1).
- Clean Shear Valve (VP-48).
Problem
Corrected
?
- From Main Menu, press
„ DIAGNOSTICS
„ F12
„ F1
„ SET POINT ENTRY
N O - Verify RBC/PLT Threshold
is 550.
- From Main Menu, press
- Verify WBC Sample is
being staged to Optical
Flow Cell.
- Verify operation of Valve 53
and 55 (VP-10) and tubing.
Replace as necessary (PL4.1), (TM-1).
- Verify 0 o and 10 o Log
Amplifiers (VP-28).
- Verify operation of WBC
Syringe (VP-6), (VP-7).
- Verify 0 o and 10 o Gain (VP26), (VP-27).
- Clean Shear Valve (VP-48).
„ DIAGNOSTICS
„ F12
„ F1
„ SET POINT ENTRY
NO
- Verify WBC Threshold is
360.
- Verify WBC Gain (VP-23).
- Replace MAM (PL-12).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).
- Replace MAM (PL-12).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).
YES
Problem
Corrected
?
Total Call
YES
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 21
5#7URXEOHVKRRWLQJ • IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 8 of 11)
12
- Verify NOC Sample is being
staged to Optical Flow Cell.
- Verify operation of Valve 41
(VP-10) and tubing. Replace as
necessary (PL-4.1), (TM-1).
- From Main Menu, press
„ DIAGNOSTICS
„ F12
13
14
- Clean Laser Bench (VP-43).
- From Main Menu, press
- Clean, debubble, and wet
Optical Flow Cell (VP-44),
(VP-45).
„ DIAGNOSTICS
- Verify beam alignment (VP20), (VP-21).
„ SET POINT ENTRY
- Replace Optical Flow Cell
(PL-10).
„ F1
- Replace MAM (PL-12).
„ SET POINT ENTRY
- Replace CPU/DCM (PL-13).
- Verify NOC Threshold is 400.
- Perform NOC Gain Adjustment
(VP-25).
„ F12
„ F1
- Verify RBC/PLT Threshold is
550.
- Verify 0 o and 10 o Log Amplifiers
(VP-28).
- Verify 0 o and 10 o Gain (VP-26),
(VP-27).
- Replace MAM (PL-12).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 22
5#7URXEOHVKRRWLQJ • IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 9 of 11)
15
16
- From Main Menu, press
- Verify operation of WBC
Syringe (VP-6), (VP-7).
„ DIAGNOSTICS
„ F12
- Clean Shear Valve (VP-48).
„ SET POINT ENTRY
- Verify WBC Threshold is
360.
- Verify WBC Gain (VP-23).
Problem
Corrected
?
„ DIAGNOSTICS
„ F12
„ F1
NO
- From Main Menu, press
- Replace MAM (PL-12).
„ F1
„ SET POINT ENTRY
- Verify NOC Threshold is
400.
- Perform NOC Gain
Adjustment (VP-25).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).
YES
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 23
5#7URXEOHVKRRWLQJ • IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 10 of 11)
17
- Verify stable laser power
(VP-46).
- Verify samples are being staged to
Optical Flow Cell by Peri-pump.
Troubleshoot as necessary.
YES
- Verify Sheath Reservoir is filling
properly.
- Verify Pressure 2 (VP-16) and
proper Sheath flow to Optical Flow
Cell. Troubleshoot as necessary.
NO
Power Stable
- Replace MAM (PL-12).
?
- Replace SPM (PL-12).
- Replace CPU/DCM (PL-13).
- Replace Vent Accumulator Filter
(PL-4.2).
NO
- Verify operation of Sample
Injection Syringe (VP-6), (VP-7).
Troubleshoot as necessary.
- Connect new Laser Head to
Laser Power Supply and
verify power (See Warning).
If good, replace Laser Head
(PL-10). If not, replace Laser
Power Supply (PL-2).
Problem
Corrected
?
- Clean, debubble, and wet
Optical Flow Cell (VP-44),
(VP-45).
- Replace Optical Flow Cell
(PL-10).
- Replace In-Line Filter (PL-4.2).
NO
- Clean Laser Bench (VP-43).
'DQJHU=#+LJK#9ROWDJH#00#$VVXUH#WKH#SRZHU#WR#WKH#
'DQJHU
LQVWUXPHQW#LV#RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#
WKH#ODVHU#FRQQHFWRU#ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#
PDOH#SLQV1#6KRUW#PDOH#SLQV#WR#&KDVVLV1
YES
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 24
5#7URXEOHVKRRWLQJ • IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 11 of 11)
18
- From Main Menu,
press
- From Main Menu, press
„ DIAGNOSTICS
„ DIAGNOSTICS
- Verify operation of Valve 54 (VP-10)
and tubing . Replace as necessary
(PL-4.1) , (TM-1) .
- Clean Shear Valve (VP-48) .
„ F12
„ F12
- Verify RBC/PLT Sample is being
staged to Optical Flow Cell.
„ F1
„ F1
NO
„ SET POINT ENTRY
„ SET POINT ENTRY
- Verify RBC/PLT
Threshold is 550.
- Verify WBC Threshold is
360.
- Replace MAM (PL-12) .
- Replace SPM (PL-10) .
19
NO
- Verify operation of WBC
Syringe (VP-6), (VP-7).
- Replace CPU/DCM (PL-13) .
20
- Clean Shear Valve (VP-48) .
Problem
Corrected
- Verify NOC Sample is being staged
to Optical Flow Cell.
?
YES
Total Call
Problem
Corrected
?
YES
- Verify operation of Valve 41 (VP-10)
and tubing. Replace as necessary
(PL-4.1) , (TM-1) .
- From Main Menu, press
„ DIAGNOSTICS
„ F12
„ F1
„ SET POINT ENTRY
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
- Verify NOC Threshold is 400.
Part Number 9140300 − August 1998
2 - 25
5#7URXEOHVKRRWLQJ • IP-7 Reagent Empty
IP-7 Reagent Empty (Page 1 of 5)
Start
- Verify reagents, type and
expiration.
- Ensure reagent
containers are connected
to the correct tubing and
input ports.
- Ensure sinkers are on
lines and lines are at
bottom of containers.
- Ensure reagent lines are
not crimped or clogged.
HGB Lyse
Empty
?
NO
YES
YES
- Open Right Front Door
and observe HGB/
WBC Syringe.
1
WBC Lyse
Empty
?
NO
- INITIALIZE and PRIME
instrument.
Diluent/Sheath
Empty
YES
- Open Left Front Door
and observe Diluent and
Sheath Reservoirs.
?
NO
2
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 26
5#7URXEOHVKRRWLQJ • IP-7 Reagent Empty
IP-7 Reagent Empty (Page 2 of 5)
1
From Main Menu,
press
YES
Syringe
Filling
Properly
?
„ RUN
„ CLEAR FAULT
Syringe
Moving
Properly
?
NO
- Verify operation and tubing
in valves 28 for HGB, 23
for WBC. (VP-10) See
Figure. Replace as
necessary (PL-4.1), (TM-1).
YES
- Remove Top Cover and
observe Reagent Sensor
Board. See Figure
- Inspect and seat
connectors on J1 of
Reagent Sensor Board
and J26 of FCM.
- Replace FCM (PL-14.1).
- Replace SDM2 (PL-15)
Floss Tubing
Up and Down
Ensure Tubing
is Seated in Slot
NO
- Clean/replace syringe (PL-7).
- Verify Syringe Driver
operation (VP-6), (VP-7).
- Replace Syringe Driver
Assembly (PL-7), (RR-11).
- Verify operation and tubing in
valves 24 for HGB, 25 for WBC.
(VP-10) See Figure. Replace as
necessary (PL-4.1), (TM-1).
- Replace SDM2 (PL-15).
32098
- Replace MPM (PL-11).
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 27
5#7URXEOHVKRRWLQJ • IP-7 Reagent Empty
IP-7 Reagent Empty (Page 3 of 5)
2
Diluent
Not Filling
Properly
?
From Main Menu, press
„ RUN
„ CLEAR FAULT
- Observe the Diluent and Sheath
Reservoirs and verify that both fill to
the Sensor Electrodes and
immediately stop. See Figure
5
YES
6
NO
NO
Both
Filling
Properly
?
YES
Note: The "Diluent/Sheath Empty"
message can be generated by the In-line
Diluent/Sheath Sensor, the Diluent
Reservoir Level Sensor, or the Sheath
Reservoir Level Sensor.
Sheath
Not Filling
Properly
?
NO
YES
3
NO
Neither
Filling
Properly
?
YES
Diluent Filling
Past Electrode
?
YES
7
YES
NO
Fill
To
H e re
Sheath Filling
Past Electrode
32099
?
4
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 28
5#7URXEOHVKRRWLQJ • IP-7 Reagent Empty
IP-7 Reagent Empty (Page 4 of 5)
3
- Remove Top Cover and
observe Reagent Sensor
Board. See Figure
- Inspect and seat
connectors on J1 of
Reagent Sensor Board
and J26 of FCM.
- Replace FCM (PL-14.1).
4
5
- Ensure Diluent/Sheath plumbing
is free of restrictions.
- Ensure Vac 1 line to reservoir
is free of restrictions.
- Ensure there is no saline bridge at
reservoir connectors.
- Ensure there is no saline
bridge at reservoir connector.
- Verify the Vac 1 level (VP-16)
and verify Vac 1 retention (VP15).
- Verify operation of Valves 62
and 63 (VP-10). Replace as
necessary (PL-4.1)
- Ensure Vac 1 lines to reservoirs is
free of restrictions.
- Verify operation of Check
Valve Replace as necessary
(TM-1).
- Verify operation of Valves 61, 62,
63, and 64 (VP-10). Replace as
necessary (PL-4.1).
- Verify operation of Check Valves.
Replace as necessary (TM-1).
- Verify Reservoir is not
cracked or leaking Replace
as necessary (PL-4.2).
- Replace FCM (PL-14.1).
6
- Ensure Vac 1 line to
reservoir is free of
restrictions.
- Ensure there is no saline
bridge at reservoir
connector.
- Verify operation of Valves 61
and 64 (VP-10). Replace as
necessary (PL-4.1).
- Verify operation of Check
Valve. Replace as
necessary (TM-1).
- Verify Reservoir is not
cracked or leaking. Replace
as necessary (PL-4.2).
- Replace FCM (PL-14.1).
- Verify Reservoirs are not cracked
or leaking. Replace as necessary
(PL-4.2).
- Replace FCM (PL-14.1).
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 29
5#7URXEOHVKRRWLQJ • IP-7 Reagent Empty
IP-7 Reagent Empty (Page 5 of 5)
7
- Clean sensor contacts
(Electrodes and
connector) at top of
Reservoir.
- Remove pins 1 and 4
and clean and crimp
metal contacts so they
are touching. See Figure
- If pins 1 and 4 are
damaged, replace with
pins 2 and 3.
32100
- Reseat Sensor Cables
(CDM 2 J8 for Diluent, or
CDM 2 J10 for Sheath.
- Replace FCM (PL-14.1).
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 30
5#7URXEOHVKRRWLQJ • IP-8 Optical Backgrounds Too High (Page 1 of 1)
IP-8 Optical Backgrounds Too High (Page 1 of 1)
Start
- Perform the following in order and verify
backgrounds after each action taken.
- From Main Menu, press
- Verify Background Counts
(VP-56).
„ DIAGNOSTICS
NO
- Perform Extended Autoclean.
„ F12
- Clean Shear Valve (VP-48).
„ F1
- Clean and debubble Optical Flow Cell
(VP-44).
„ SET POINT ENTRY
WBC Only
Too High
?
- Verify RBC/PLT Threshold is 550.
- From Main Menu, press
- Clean/replace Diluent and Sample
Injection Syringes (PL-7).
„ SPECIAL PROTOCOLS
- Replace In-line Sheath Filter (PL-4.2).
„ MORE
YES
NO
„ REAGENT RESERVOIR
- Follow on-screen instructions and press
- Ensure RBC/PLT Mixing Chamber is
being rinsed and drained properly.
- Clean Laser Bench (VP-43).
„ EMPTY DIL/SHEATH
- From Main Menu, press
- Replace Diluent Container and press
„ DIAGNOSTICS
„ FILL DIL/SHEATH
„ F12
- Run multiple backgrounds and verify.
„ F1
„ SET POINT ENTRY
- Verify WBC Threshold is 360.
- Replace WBC Lyse Container.
- Clean/replace WBC Syringe (PL-7).
Backgrounds
OK
- Ensure WBC Mixing Chamber is
being rinsed and drained properly.
?
- INITIALIZE and PRIME Instrument.
YES
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 31
5#7URXEOHVKRRWLQJ • IP-9 Histogram Problems (Page 1 of 1)
IP-9 Histogram Problems (Page 1 of 1)
Start
- Perform the following in order
and verify histograms after
each action taken.
- Verify reagents; type and
expiration date.
- Ensure reagents are at
room temperature.
- Verify Backgrounds (VP-56).
WBC Only
- Ensure RBC/PLT and/or
WBC Mixing Chambers are
being rinsed and drained
properly.
?
NO
- Perform Extended Autoclean.
- Clean Shear Valve (VP-48).
Backgrounds
OK
NO
Go to
(IP-8) N O
?
- Clean, debubble, and wet
Optical Flow Cell (VP-44),
(VP-45).
NO
- Fine align Optical Flow Cell
(VP-20).
YES
YES
- Verify WBC Gains
(VP-23).
- Verify 0 o and 10 o Log
Amplifiers (VP-28).
- Verify 0 o and 10 o Gains
(VP-26), (VP-27).
- Clean Laser Bench (VP-43).
- Replace WBC Lyse for WBC
problem or Diluent for RBC/
PLT problem.
- Clean/replace Diluent, WBC
Lyse, and Sample Injection
Syringes (PL-7).
- Replace In-line Sheath Filter
(PL-4.2).
Problem
Corrected
?
YES
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Problem
Corrected
?
YES
Total Call
Part Number 9140300 − August 1998
2 - 32
5#7URXEOHVKRRWLQJ • IP-10 Controls Out
IP-10 Controls Out (Page 1 of 2)
Start
NO
- Verify reagents; type and
expiration date.
- Ensure reagents are at room
temperature and have not
been previously frozen.
- Ensure Controls and
Calibrators have not been
frozen or heat stressed.
Q.C. OK
YES
?
Total Call
- Perform Autoclean.
- From Main Menu, press
„ SPECIAL PROTOCOLS
- Open fresh Commercial
Control, and run Q.C.
„ MORE
„ AUTOCLEAN
- Verify Backgrounds (VP-56).
- Check Control Lot #
- Use current Control Insert
Sheet to verify Q.C means/
limits are correct if customer
has not established their own
means.
- From Main Menu, press
„ SETUP
„ Q.C. SETUP MENU
„ Q.C. LIMITS
- Edit as necessary.
- Run Q.C.
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
YES
All
Backgrounds
OK
?
NO
Results
Acceptable
NO
1
?
YES
Total Call
Go to (IP-8).
Part Number 9140300 − August 1998
2 - 33
5#7URXEOHVKRRWLQJ • IP-10 Controls Out
IP-10 Controls Out (Page 2 of 2)
1
NO
- Clean Shear Valve (VP-48).
Precision
In Spec
?
- Verify System Precision
- From Main Menu, press
„ RUN
„ SPECIMEN TYPE
YES
- Recalibrate out-ofrange parameter(s)
(VP-58).
- Determine which
parameter(s) are out-ofrange, and go to (IP-15)
or (IP-16) for HGB and
correct.
- For indices (MCH or
MCHC), determine which
parameter (HGB, MCV,
or RBC is imprecise and
troubleshoot accordingly.
MCH = HGB X10
RBC
- Select empty Q.C. File
„ Q.C. SPECIMEN
- Review CV% (VP-57).
MCHC = HGB X 100
MCV X RBC
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 34
5#7URXEOHVKRRWLQJ • IP-11 Vacuum Accumulator #1 Wet
IP-11 Vacuum Accumulator #1 Wet (Page 1 of 2)
Start
- Reinitialize Analyzer
From Main Menu:
Liquid Exiting
Analyzer
YES
?
„ DIAGNOSTICS
„ MORE
„ INITIALIZATION
NO
- Verify that Waste Chamber 2 is
not overflowing. If overflowing,
verify Pressure Valve 18 (VP-10).
Replace as necessary (PL-4.1).
Check output Check Valve.
Replace as necessary (TM-1).
- Ensure operation of Waste
Chamber 2 Trap and Trap Output
Check Valve. Replace as
necessary (PL-4.2) and (TM-1).
- Perform Vacuum
Accumulator #2 Rinsing
Procedure (VP-60)
Press:
„ MORE
- Observe the Waste Line into
External Waste Container and
press:
„ DRAIN ACCUMULATORS
Problem
Corrected
NO
?
YES
1
- Perform Vacuum
Accumulator #2 Rinsing
Procedure (VP-60)
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 35
5#7URXEOHVKRRWLQJ • IP-11 Vacuum Accumulator #1 Wet
IP-11 Vacuum Accumulator #1 Wet (Page 2 of 2)
1
- Check Cable and replace
if necessary (PL-5)
- Remove connector from
J11 on VPM and check
resistance between
connector pins. Must be
greater than 20
Megohms. See Figure
- Replace Accumulator
(PL-5)
NO
J10
Vac 1 Acc
J11
Vac 2 Acc
VPM
BD
> 20 Megohms
?
YES
32003
- Replace VPM (PL-5)
- Replace FCM (PL-14.1)
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 36
5#7URXEOHVKRRWLQJ • IP-12 Vacuum Accumulator #2 Wet
IP-12 Vacuum Accumulator #2 Wet (Page 1 of 2)
Start
- Reinitialize Analyzer
From Main Menu:
Liquid Exiting
Analyzer
YES
?
„ DIAGNOSTICS
„ MORE
„ INITIALIZATION
NO
- Verify that Waste Chamber 2 is
not overflowing. If overflowing,
verify Pressure Valve 18 (VP-10).
Replace as necessary (PL-4.1).
Check output Check Valve.
Replace as necessary (TM-1).
- Ensure operation of Waste
Chamber 2 Trap and Trap Output
Check Valve. Replace as
necessary (PL-4.2) and (TM-1).
- Perform Vacuum
Accumulator #2 Rinsing
Procedure (VP-60)
Press:
„ MORE
- Observe the Waste Line into
External Waste Container and
press:
„ DRAIN ACCUMULATORS
Problem
Corrected
NO
?
YES
1
- Perform Vacuum
Accumulator #2 Rinsing
Procedure (VP-60)
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 37
5#7URXEOHVKRRWLQJ • IP-12 Vacuum Accumulator #2 Wet
IP-12 Vacuum Accumulator #2 Wet (Page 2 of 2)
1
- Check Cable and replace
if necessary (PL-5)
- Remove connector from
J11 on VPM and check
resistance between
connector pins. Must be
greater than 20
Megohms. See Figure
- Replace Accumulator
(PL-5)
NO
J10
Vac 1 Acc
J11
Vac 2 Acc
VPM
BD
> 20 Megohms
?
YES
32003
- Replace VPM (PL-5)
- Replace FCM (PL-14.1)
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 38
5#7URXEOHVKRRWLQJ • IP-13 HGB Problems
IP-13 HGB Problems (Page 1 of 2)
Start
"0", Low, High, or
Erratic
- Verify proper Reagents and
Expiration Date.
HGB Only
?
From Main Menu, Press:
„ RUN
„ SPECIMEN TYPE
„ FRAGILE WBC
Both
- Run a Normal Control five
times and record the NOC
and HGB values.
- Compare the NOC and HGB
results to the Data Sheet.
- Perform HGB Cleaning
Procedure (VP-49).
- Remove, inspect, and clean Shear
Valve.
- Perform Vacuum & Pressure Level
Verification/Adjustment (VP-16).
- Verify tubing and operation of Valves
51, 28, 24, 33, 93, and 26 (VP-10)
and replace as necessary (PL-4.1).
From Main Menu, Press:
„ DIAGNOSTICS
„ RAW DATA SUMMARY
- Run five background counts
and observe the HGB Sample
and Reference Readings.
- Verify operation of HGB Lyse and
RBC Diluent Syringes (VP-6), (VP-7)
and replace as necessary (PL-7).
1
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 39
5#7URXEOHVKRRWLQJ • IP-13 HGB Problems
IP-13 HGB Problems (Page 2 of 2)
2
1
5
Total Call
Select path from Chart below.
Reference
Sample
2
1950-2050
1950-2050
< 1950
< 1950
3
> 2050
>2050
3
Erratic
1950-2050
4
≈ 1600
1950-2050
4
Erratic
Erratic
5
≈0
≈0
- Verify connector at J18 of FCM.
3
- Replace HGB Flow Cell (PL-4.2).
- Replace FCM (PL-14.1).
- Replace CPU/DCM (PL-13).
- Perform HGB Adjustment
(VP-5)
4
5
- Verify tubing, operation (VP-10),
and flow through Valve 94 and
replace as necessary (TM-1),
(PL-4.1).
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 40
5#7URXEOHVKRRWLQJ • IP-14 X-B Errors
IP-14 X-B Errors (Page 1 of 3)
Effect Of RBC, MCV, & HGB On X-B Parameters
Start
- Clean Fan Filters and verify all Fans are running
properly.
- Ensure there is at least 6" of clearance behind and
on the left side of Instrument.
X-B Parameter
RBC
RBC
MCV
NA
NA
MCH
NA
- Review patient result in Datalog, and look for groups
( 3 or more) of abnormal MCV, RBC, or HGB.
-If MCV shifts, ask Customer for possible causes,
such as Oncology or Geriatric Patients.
If MCH or MCHC errors:
- See table to determine which parameter (HGB,
RBC, MCV) is bad.
Note: HCT = RBC X MCV/10
MCV
HGB
HGB
NA
NA
NA
MCHC
- Ensure Instrument is not subject to drastic
temperature variations (direct sunlight, heating/air
conditioning ducts).
- If Fan Filters were clogged and then cleaned, allow a
15 minute temperature stabilization period.
MCV
Controls at or
Near 2 SD
of mean
?
NO
- Review Customer Maintenance Log.
- Review Maintenance Procedures and
Dates.
- Review X-B Results.
- Determine if Maintenance was
performed just before X-B Shift.
YES
Go to (IP-10)
Controls Out.
1
MCH = HGB X 10/RBC
MCHC = (HGB X 100)/(RBC X MCV)
- Review Q.C. Logs for all 3 Control Levels.
- Verify RBC, HGB, and MCV Control Levels are not
at the high or low end of range.
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 41
5#7URXEOHVKRRWLQJ • IP-14 X-B Errors
IP-14 X-B Errors (Page 2 of 3)
1
- Verify Reagent, Type and
Expiration Date.
Shift
After
Maintenance
?
Reagent
Change
Required
?
YES
- Repeat Maintenance
Procedures
performed before X-B
Shift.
- Monitor X-B for 5 Data
Points (100 Samples).
NO
X-B In
Range
YES
- Monitor X-B for 5
Data Points (100
Samples).
2
?
YES
X-B In
Range
Total Call
NO
?
YES
2
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 42
5#7URXEOHVKRRWLQJ • IP-14 X-B Errors
IP-14 X-B Errors (Page 3 of 3)
2
- Verify System Precision
- From Main Menu, press
„ RUN
„ SPECIMEN TYPE
- Select empty Q.C. File
„ Q.C. SPECIMEN
- Run one Patient 10
times.
- Review CV% (VP-57) .
All
Parameters
OK
?
NO
- Determine which
parameter(s) are out-ofrange, and go to (IP-15) or
(IP-16) for HGB and correct.
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
YES
- Verify Calibrator Insert
Sheet matches Current
Calibrators.
- Perform System
Calibration (VP-58) .
Part Number 9140300 − August 1998
2 - 43
5#7URXEOHVKRRWLQJ • IP-15 Optical Parameters Imprecision
IP-15 Optical Parameters Imprecision (Page 1 of 3)
Start
- Verify Background Counts
(VP-56).
- Clean Fan Filters and verify all Fans are
running properly.
- Ensure there is at least 6" of clearance
behind and on the left side of Instrument.
- Ensure Instrument is not subject to drastic
temperature variations (direct sunlight,
heating/air conditioning ducts).
- If Fan Filters were clogged and then
cleaned, allow a 15 minute temperature
stabilization period.
- Perform Autoclean
From Main Menu, press
- Verify System Precision.
- From Main Menu, press
„ SPECIMEN TYPE
- Select empty Q.C. File
- Run one Patient 10 times.
- Review CV% (VP-57).
- Manually clean, debubble, and wet Optical
Flow Cell (VP-44), (VP45).
- Clean Optics Bench (VP-43).
Go to (IP-8).
YES
2
YES
3
All
Imprecise
NO
Problem
Corrected
?
RBC/PLT
Only
?
YES
- Replace In-line Sheath Filter (PL-4.2).
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
NO
YES
?
„ MORE
„ AUTOCLEAN
?
NO
„ Q.C. SPECIMEN
„ SPECIAL PROTOCOLS
- Clean Shear Valve (VP-48).
Backgrounds
OK
„ RUN
NO
1
Part Number 9140300 − August 1998
2 - 44
5#7URXEOHVKRRWLQJ • IP-15 Optical Parameters Imprecision
IP-15 Optical Parameters Imprecision (Page 2 of 3)
1
2
- Replace WBC Lyse Container.
YES
„ F12
„ F1
„ SET POINT ENTRY
WBC
Only
?
- Verify WBC Threshold is 360.
- Ensure WBC Mixing Chamber is
being rinsed and drained
properly.
- Verify operation of WBC Lyse
Syringe (VP-6) , (VP-7) .
Troubleshoot as necessary.
- Verify/adjust WBC Gain (VP-23) .
NO
NOC
Only
?
- Replace Shear Valve Sections
(PL-9.1) .
YES
- Ensure HGB Mixing
Chamber is being rinsed
and drained properly.
- From Main Menu, press
„ DIAGNOSTICS
NO
- Replace MAM (PL-12) .
- Replace SPM (PL-10) .
- Verify samples are being staged to
Optical Flow Cell by Peri-pump.
Troubleshoot as necessary.
- Verify Sheath Reservoir is filling
properly.
- Verify Pressure 2 (VP-16) and
proper Sheath flow to Optical Flow
Cell. Troubleshoot as necessary.
- Verify operation of Sample
Injection Syringe (VP-6) , (VP-7 ).
Troubleshoot as necessary.
- Replace Optical Flow Cell (PL-10) .
- Replace Shear Valve Sections
(PL-9.1) .
- Replace MAM (PL-12) .
- Replace SPM (PL-10) .
- Replace CPU/DCM (PL-13) .
- Replace CPU/DCM (PL-13) .
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 45
5#7URXEOHVKRRWLQJ • IP-15 Optical Parameters Imprecision
IP-15 Optical Parameters Imprecision (Page 3 of 3)
3
- Replace Diluent Container.
- From Main Menu, press
„ DIAGNOSTICS
„ F12
„ F1
„ SET POINT ENTRY
- Verify RBC/PLT Threshold is 550.
- Ensure RBC/PLT Mixing Chamber is
being rinsed and drained properly.
- Verify 0 o and 10 o Log Amplifiers (VP-28).
- Verify 0 o and 10 o Gain (VP-26), (VP-27).
- Replace Shear Valve Sections (PL-9.1).
- Replace MAM (PL-12).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 46
5#7URXEOHVKRRWLQJ • IP-16 HGB Imprecision (Page 1 of 1)
IP-16 HGB Imprecision (Page 1 of 1)
Start
- Clean Fan Filters and verify all Fans are
running properly.
- Ensure there is at least 6" of clearance
behind and on the left side of
Instrument.
- Ensure Instrument is not subject to
drastic temperature variations (direct
sunlight, heating/air conditioning ducts).
- If Fan Filters were clogged and then
cleaned, allow a 15 minute temperature
stabilization period.
HGB CVs
OK
- Ensure HGB Flow Cell is being
rinsed, drained, and vented
properly. Verify Valves 51, 94, 93,
(VP-10) and tubing . Replace as
necessary (PL-4), (TM-1).
?
YES
NO
- Clean HGB Lyse Syringe.
Total Call
NO
- Replace HGB Lyse
Container>
- Verify HGB Lyse Syringe (VP-6),
(VP-7). Replace as necessary
(PL-7).
- Inspect and re-seat Cable at J18
of FCM (PL-4.2).
- Clean Shear Valve (VP-48).
- Replace HGB Flow Cell (PL-4.2).
- Manually clean HGB Flow Cell (VP-49).
- Replace FCM (PL-14.1).
- Verify System Precision.
NO
- Replace CPU/DCM (PL-13).
- From Main Menu, press
„ RUN
„ SPECIMEN TYPE
- Select empty Q.C. File
„ Q.C. SPECIMEN
- Run one Patient 10 times.
HGB CVs
OK
?
YES
- Review HGB CV% (VP-57).
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 47
5#7URXEOHVKRRWLQJ • IP-17 Primary Vacuum/Pressure Isolation,
5
4
YES
3
?
2
NO
Vac
Constantly
Running
NO
From Main Menu:
„ DIAGNOSTICS
„ MORE
„ INITIALIZATION
1
- Reinitialize Analyzer
9P SI
4
YES
12P SI
- Remove Left Side Cover and
extend Air Supply (RR-7) to
observe pumps. (Vac-Front,
Pres-Rear)
TO
FLO W PA N E L
To S L
Pres
Constantly
Running
6
To S L
7
Start
5P SI 12"H g 8"Hg 12P SI 12"H g
IP-17 Primary Vacuum/Pressure Isolation, (Page 1 of 9)
3 2 00 4
?
NO
Both
Constantly
Running
5
- Disconnect tubing from
positions 6 and 7 on Air
Supply Manifold, and
observe Pumps and VPM
Pump LEDs. See Figures
?
YES
32005
- Observe LEDs. See
Figure
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
1
6
Part Number 9140300 − August 1998
2 - 48
5#7URXEOHVKRRWLQJ • IP-17 Primary Vacuum/Pressure Isolation,
IP-17 Primary Vacuum/Pressure Isolation, (Page 2 of 9)
1
All Valves
Close Then
Open
?
Both LEDs
ON
NO
2
?
+12 VNR
Fuse (Top)
YES
NO
- Replace Pump Relay
Board (PL-5)
32006
- Check +12V UR and Fuse.
See Figure
YES
- Replace if necessary (PL-1)
Check fuse again
NO
- Reinitialize Analyzer
From Main Menu:
„ DIAGNOSTICS
„ MORE
Problem
Corrected
Fuse
Blown
?
?
„ INITIALIZATION
- Observe Flow Panel
valves
NO
8
YES
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
YES
- Replace Power Supply
(PL-1)
Part Number 9140300 − August 1998
2 - 49
5#7URXEOHVKRRWLQJ • IP-17 Primary Vacuum/Pressure Isolation,
IP-17 Primary Vacuum/Pressure Isolation, (Page 3 of 9)
2
Note: If the +24 UR is approximately
the same as the +12 UR, the +24V
Fuse is blown or the +24V Power
Supply is defective.
- Check +24V UR (28.9V37.7V) and Fuse
(Bottom Fuse)
- While observing VPM
Solenoid LEDs (See
Figure) press:
„ INITIALIZATION
- Replace Fuse if
necessary (PL-1)
NO
NO
- Check Fuse again
Problem
Corrected
LEDs Bright
Then Dim When
Activated
?
YES
?
Fuse
Blown
YES
?
Total Call
YES
NO
3
- Replace Power Supply
(PL-1)
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
8
32007
2 - 50
5#7URXEOHVKRRWLQJ • IP-17 Primary Vacuum/Pressure Isolation,
TO
FL O W PA N E L
3
P inch
1
Pumps
Stop
?
NO
12P SI
9P SI
YES
- Pinch tubing at positions
6 and 7 that supply 12
psi and 12" Hg to Sample
Loader
2
- Pinch tubing as shown in
Figure
4
- Unpinch tubing
5P SI 12"H g 8"H g 12P SI 12"H g
5
To S L
6
To S L
3
7
IP-17 Primary Vacuum/Pressure Isolation, (Page 4 of 9)
32008
Pumps
Stop
NO
?
- Inspect 12 psi and 12" Hg
plumbing and
Accumulators. Replace
as necessary (PL-5),
(TM-2)
- Inspect Vent Valves 1 and
4. Replace as necessary
(PL-5)
- Replace VPM (PL-5)
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
YES
- Inspect Sample Loader
plumbing and 3-Way
Valves. Replace as
necessary (PL-18.3),
(TM-3)
- Ensure Valves 31 and 32,
97 and 98, 17 and 18 are
not open at the same
time.
- Inspect Flow Panel 12 psi
and 12" Hg plumbing for
leaks.
Part Number 9140300 − August 1998
2 - 51
5#7URXEOHVKRRWLQJ • IP-17 Primary Vacuum/Pressure Isolation,
IP-17 Primary Vacuum/Pressure Isolation, (Page 5 of 9)
4
Pump
Stops
YES
- Unpinch tubing and pinch
at position 6 on Manifold.
?
- Observe Pressure Pump
LED. See Figure Page 1
NO
Pump
Stops
?
YES
- Replace VPM (PL-5)
To S L
To S L
9P S I
2
P inch
1
- Pinch tubing See Figure
this page
- Inspect Sample Loader
plumbing and 3-Way
Valves. Replace as
necessary (PL-18.3),
(TM-3)
3
4
5
- Replace Pump Relay
Board (PL-5)
TO
FL O W PA N E L
NO
YES
- Inspect Flow Panel 12 psi
plumbing for leaks.
5P S I 12"H g 8"H g 12P S I 12"H g
- Inspect Vent Valve 1.
Replace as necessary
(PL-5)
12P S I
?
7
LED
ON
NO
6
- Inspect 12 psi plumbing
and Accumulator.
Replace as necessary
(PL-5), (TM-2)
32 0 09
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 52
5#7URXEOHVKRRWLQJ • IP-17 Primary Vacuum/Pressure Isolation,
IP-17 Primary Vacuum/Pressure Isolation, (Page 6 of 9)
5
Pump
Stops
YES
- Unpinch tubing and pinch
at position 7 on Manifold.
?
- Observe Vacuum Pump
LED. See Figure Page 1
NO
Pump
Stops
?
- Inspect 12" Hg plumbing
and Accumulator.
Replace as necessary
(PL-5), (TM-2)
- Replace VPM (PL-5)
To S L
To S L
9P SI
2
Pinch
1
- Pinch tubing. See Figure
- Inspect Sample Loader
plumbing and 3-Way
Valves. Replace as
necessary (PL-18.3),
(TM-3)
3
4
5
- Replace Pump Relay
Board (PL-5)
TO
FLO W PAN E L
YES
YES
5P SI 12"H g 8"H g 12P SI 12"H g
NO
- Inspect Flow Panel 12"
Hg plumbing for leaks.
12P SI
- Inspect Vent Valve 4.
Replace as necessary
(PL-5)
7
?
6
LED
ON
NO
32010
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 53
5#7URXEOHVKRRWLQJ • IP-17 Primary Vacuum/Pressure Isolation,
IP-17 Primary Vacuum/Pressure Isolation, (Page 7 of 9)
6
- Replace VPM
(PL-5)
NO
- Reseat connectors VPM J18, PRM
J1, J2, J3, J4
Neither
Pump
Runs
YES
LEDs
ON
- Replace VPM
(PL-5)
NO
NO
YES
NO
Pres
Doesn't
Run
VAC LED
ON
NO
- Replace PRM (PL-xx)
- Reseat connectors VPM J18, PRM
J1, J3
YES
?
?
- Check Fuse on PRM. Replace if
necessary (PL-5)
- Verify 115VAC to PRM
?
?
VAC
Doesn't
Run
YES
- Check 115VAC across Pins 1 and 2
on PRM P3. IF present, replace Vac
Pump. If not, replace PRM (PL-5)
- Replace VPM
(PL-5)
- Reseat connectors VPM J18,
PRM J1, J2
YES
Pres LED
ON
?
?
YES
- Check 115VAC across Pins 1 and
2 on PRM P2. IF present, replace
Pres Pump. If not, replace PRM
(PL-5)
7
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 54
5#7URXEOHVKRRWLQJ • IP-17 Primary Vacuum/Pressure Isolation,
IP-17 Primary Vacuum/Pressure Isolation, (Page 8 of 9)
7
NO
Both
OK
- Re-connect tubing to
positions 6 and 7 (6-Red,
7-Green)
- Replace Pump Head
- If problem persists,
replace Pump Assembly
?
Pump Head:
Pressure (PL-5)
YES
Vacuum
(PL-5)
Pump Assy:
- Perform Vacuum and
Pressure Level
Verification/Adjustment
(VP-16)
- Observe Vacuum and
Pressure Pumps and
time the duration between
run cycles.
- Perform Vacuum and
Pressure Recovery
Verification (VP-17)
Pres
Cycles
< 2 min
YES
Pressure (PL-5)
Vacuum
NO
Vac
Cycles
< 2 min
?
(PL-5)
NO
?
Total Call
See Pressure Leaks (IP-18)
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
See Vacuum Leaks (IP-19)
YES
Part Number 9140300 − August 1998
2 - 55
5#7URXEOHVKRRWLQJ • IP-17 Primary Vacuum/Pressure Isolation,
IP-17 Primary Vacuum/Pressure Isolation, (Page 9 of 9)
8
To
C D M 2-J3
FC M -J5
- If the Fuse continues to blow,
assemblies on the +12V UR or
+24V UR must be disconnected
one at a time until the shorted
assembly is found and
replaced. (See Figures)
- Power-down instrument and
connect Meter between TP2+12V or TP1-+24V and TP4 on
PSM and remove connectors
until short is found
- Replace defective assembly and
verify proper operation
To
S D M /C D M -J3
J1
UR RET
1
3
4 +1 2 V U R
5 +2 4 V U R
J2
UR RET
1
3
4 +1 2 V U R
+2 4 V U R
5
B a ck
C D M 2 & S D M /C D M P o w er C on n ecto rs
J1
J3
+2 4 V U R
5
1
Fro m
+1 2 V U R
To
4
2
P.S .
SDM A
UR RET
J1/J2 3
3
1
PSM
J2
1
2
3
J3
To
S h ea r
Valve
D rive r-J3
+2 4 V U R
1
2 RET
N o te: J4 an d J5 a re no t o n
S D M /C D M
To
S D M 9 -J1 0
J4
+2 4 V U R
1
+1 2 V U R
2
3 UR RET
J4
1
2
To
SDM C
3
FC M
J5
1
2
3 2 0 11
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
To
SDM B
Part Number 9140300 − August 1998
To
SDM D
3
2 - 56
5#7URXEOHVKRRWLQJ • IP-18 Pressure Leak Isolation
IP-18 Pressure Leak Isolation (Page 1 of 7)
Note: This procedure is designed to isolate leaks in the pressure plumbing. Refer to IP-17 Primary Vacuum/
Pressure Isolation for troubleshooting gross pressure failures.
Start
- Remove Left Side Cover and
extend Air Supply (RR-7) to
observe Pres Pump. (VacFront, Pres-Rear)
- Reinitialize Analyzer
From Main Menu:
„ DIAGNOSTICS
„ MORE
- Observe Pressure Pump
and time the duration
between run cycles.
Pump
Cycles
< 2 min
YES
?
„ INITIALIZATION
NO
1
Total Call
- Adjust Pressure Levels if
necessary (VP-16)
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 57
5#7URXEOHVKRRWLQJ • IP-18 Pressure Leak Isolation
IP-18 Pressure Leak Isolation (Page 2 of 7)
1
- Check +24V UR (28.9V37.7V) and Fuse
(Bottom Fuse)
- While observing VPM
Solenoid LEDs (See
Figure) press:
„ INITIALIZATION
- Replace Fuse if
necessary (PL-1)
NO
Note: If the +24 UR is approximately
the same as the +12 UR, the +24V
Fuse is blown or the +24V Power
Supply is defective.
NO
- Check Fuse again
Problem
Corrected
LEDs Bright
Then Dim When
Activated
?
YES
?
Fuse
Blown
YES
?
Total Call
YES
NO
2
- Replace Power Supply
(PL-1)
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
7
3 2 0 07
2 - 58
5#7URXEOHVKRRWLQJ • IP-18 Pressure Leak Isolation
IP-18 Pressure Leak Isolation (Page 3 of 7)
2
- Select 3 pressure levels in
real-time press:
„ MORE
„ VOLTAGE READINGS
Move cursor to each level
and press:
„ SELECT
When finished press:
„ FINISH SELECT
- Observe the 3 levels and
verify that none decrease
more than 0.2 psi in 1
minute.
3
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 59
5#7URXEOHVKRRWLQJ • IP-18 Pressure Leak Isolation
IP-18 Pressure Leak Isolation (Page 4 of 7)
3
Pres
3
OK
- Inspect tubing between
Air Supply and Flow
Panel.
YES
?
?
NO
NO
To S L
5
4
YES
3
Pres
1
OK
?
4
- Inspect 5 psi Accumulator
and plumbing on Air
Supply. Replace as
necessary (PL-5), (TM-2)
1
9P SI
2
NO
5
12P SI
?
To S L
TO
FL O W PA N E L
Pres
2
OK
- Inspect Diluent Reservoir
for leaks. Replace as
necessary (PL-4.2).
5P SI 12"H g 8"H g 12P SI 12"H g
Pres
3
OK
7
YES
- Inspect plumbing through
Valve 63 to Diluent
Reservoir.
6
NO
- Pinch tubing at position 3
of Air Supply Manifold.
See Figure
32004
YES
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Total Call
Part Number 9140300 − August 1998
2 - 60
5#7URXEOHVKRRWLQJ • IP-18 Pressure Leak Isolation
IP-18 Pressure Leak Isolation (Page 5 of 7)
4
5
- Inspect tubing between
Air Supply and Flow
Panel.
- Pinch tubing at position 2
of Air Supply Manifold.
See Figure Page 2.
YES
- Pinch tubing See Figure
- Inspect plumbing through
Valve 64 to Sheath
Reservoir.
- Inspect Sheath Reservoir
for leaks. Replace as
necessary (PL-4.2).
YES
1
P inch
NO
- Inspect Valve 1. Replace
as necessary (PL-5).
- Inspect 12 psi plumbing
and Accumulator.
Replace as necessary
(PL-5), (TM-2).
12P SI
9P SI
2
3
4
NO
6
TO
FL O W PA N E L
To S L
5P SI 12"H g 8"H g 12P SI 12"H g
7
?
5
To S L
6
?
Pres
2
OK
- Inspect 9 psi Accumulator
and plumbing on Air
Supply. Replace as
necessary (PL-5), (TM-2)
Pres
1
OK
32009
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 61
5#7URXEOHVKRRWLQJ • IP-18 Pressure Leak Isolation
IP-18 Pressure Leak Isolation (Page 6 of 7)
6
- Inspect plumbing to
Valves 32, 98, and 18.
- Unpinch tubing and pinch
at position 6 on Manifold.
- Press in on Valves 32, 98,
and 18 one at a time, if
leaking stops replace as
NO
necessary (PL-4.1)
Pres
1
OK
?
YES
- Inspect Sample Loader
plumbing and 3-Way
Valves. Replace as
necessary (PL-18.3),
(TM-3)
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 62
5#7URXEOHVKRRWLQJ • IP-18 Pressure Leak Isolation
IP-18 Pressure Leak Isolation (Page 7 of 7)
7
To
C D M 2-J3
FC M -J5
- If the Fuse continues to blow,
assemblies on the +12V UR or
+24V UR must be disconnected
one at a time until the shorted
assembly is found and replaced.
(See Figures)
- Power-down instrument and
connect Meter between TP2+12V or TP1-+24V and TP4 on
PSM and remove connectors
until short is found
- Replace defective assembly and
verify proper operation
To
S D M /C D M -J3
J1
UR RET
1
3
4 +1 2 V U R
5 +2 4 V U R
J2
UR RET
1
3
4 +1 2 V U R
+2 4 V U R
5
B a ck
C D M 2 & S D M /C D M P o w er C on n ecto rs
J1
J3
+2 4 V U R
1
5
Fro m
+1 2 V U R
To
4
2
P.S .
SDM A
UR RET
J1/J2 3
3
1
PSM
J2
1
2
3
J3
To
S h ea r
Valve
D rive r-J3
+2 4 V U R
1
2 RET
N o te: J4 an d J5 a re no t o n
S D M /C D M
To
S D M 9 -J1 0
J4
+2 4 V U R
1
+1 2 V U R
2
3 UR RET
J4
1
2
To
SDM C
3
FC M
J5
1
2
3 2 0 11
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
To
SDM B
Part Number 9140300 − August 1998
To
SDM D
3
2 - 63
5#7URXEOHVKRRWLQJ • IP-19 Vacuum Leak Isolation
IP-19 Vacuum Leak Isolation (Page 1 of 6)
Note: This procedure is designed to isolate leaks in the vacuum plumbing. Refer to IP-17 Primary Vacuum/
Pressure Isolation for troubleshooting gross vacuum failures.
Start
- Remove Left Side Cover and
extend Air Supply (RR-7) to
observe Vac Pump. (VacFront, Pres-Rear)
- Reinitialize Analyzer
From Main Menu:
„ DIAGNOSTICS
„ MORE
- Observe Vacuum Pump
and time the duration
between run cycles.
Pump
Cycles
< 2 min
YES
?
„ INITIALIZATION
NO
1
Total Call
- Adjust Vacuum Levels if
necessary (VP-16)
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 64
5#7URXEOHVKRRWLQJ • IP-19 Vacuum Leak Isolation
IP-19 Vacuum Leak Isolation (Page 2 of 6)
1
- Check +24V UR (28.9V37.7V) and Fuse
(Bottom Fuse)
- While observing VPM
Solenoid LEDs (See
Figure) press:
„ INITIALIZATION
- Replace Fuse if
necessary (PL-1)
NO
Note: If the +24 UR is approximately
the same as the +12 UR, the +24V
Fuse is blown or the +24V Power
Supply is defective.
NO
- Check Fuse again
Problem
Corrected
LEDs Bright
Then Dim When
Activated
?
YES
?
Fuse
Blown
YES
?
Total Call
YES
NO
5
3 2 0 07
2
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
- Replace Power Supply
(PL-1)
Part Number 9140300 − August 1998
2 - 65
5#7URXEOHVKRRWLQJ • IP-19 Vacuum Leak Isolation
IP-19 Vacuum Leak Isolation (Page 3 of 6)
2
- Select 2 vacuum levels in
real-time press:
„ MORE
„ VOLTAGE READINGS
Move cursor to each level
and press:
„ SELECT
When finished press:
„ FINISH SELECT
- Observe the 2 levels and
verify that none decrease
more than 0.2 psi in 1
minute.
3
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 66
5#7URXEOHVKRRWLQJ • IP-19 Vacuum Leak Isolation
IP-19 Vacuum Leak Isolation (Page 4 of 6)
3
NO
- Pinch tubing at position 5
of Air Supply Manifold.
See Figure
Vac
2
OK
- Inspect tubing between Air Supply and
Flow Panel.
- Inspect plumbing through Valve 17 to
WC-2.
YES
?
- Inspect WC-2 for leaks. Replace as
necessary (PL-4.2).
- Inspect plumbing to Valve 12.
- Press in on Valve 12. Replace as
necessary (PL-4.1).
Vac
2
OK
YES
- Inspect Vac 2
Accumulator and
plumbing on Air Supply.
Replace as necessary
(PL-5), (TM-2)
5
4
3
12P SI
1
9P SI
Total Call
2
YES
TO
FL O W PA N E L
4
To S L
?
5P SI 12"H g 8"H g 12P SI 12"H g
NO
6
To S L
NO
7
?
Vac
1
OK
32004
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 67
5#7URXEOHVKRRWLQJ • IP-19 Vacuum Leak Isolation
IP-19 Vacuum Leak Isolation (Page 5 of 6)
4
- Unpinch tubing and
pinch at position 7 on
Manifold.
- Inspect plumbing to
Flow Panel.
- Pinch tubing See Figure
YES
NO
- Inspect WC-3 and WC4 for leaks. Replace as
necessary (PL-4.2)
Vac
1
OK
Vac
1
OK
- Press in on Valves 13,
14, 21, 36, and 37 one
at a time. Replace as
necessary (PL-4.1).
?
9P SI
12P SI
1
- Inspect Vent Valve 4.
Replace as necessary
(PL-5)
YES
TO
FL O W PA N E L
5P SI 12"H g 8"H g 12P SI 12"H g
3
P inch
2
- Inspect 12" Hg
plumbing and
Accumulator. Replace
as necessary (PL-5),
(TM-2)
4
5
To S L
6
NO
7
?
To S L
- Inspect plumbing to
Valves 31 and 97.
- Inspect Sample Loader
plumbing and 3-Way
Valves. Replace as
necessary (PL-18.3),
(TM-2)
32010
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 68
5#7URXEOHVKRRWLQJ • IP-19 Vacuum Leak Isolation
IP-19 Vacuum Leak Isolation (Page 6 of 6)
5
To
C D M 2-J3
FC M -J5
- If the Fuse continues to blow,
assemblies on the +12V UR or +24V
UR must be disconnected one at a
time until the shorted assembly is
found and replaced. (See Figures)
- Power-down instrument and connect
Meter between TP2-+12V or TP1+24V and TP4 on PSM and remove
connectors until short is found
- Replace defective assembly and
verify proper operation
To
S D M /C D M -J3
To
S h ea r
Valve
D rive r-J3
J1
UR RET
1
3
4 +1 2 V U R
5 +2 4 V U R
J2
UR RET
1
3
4 +1 2 V U R
+2 4 V U R
5
B a ck
C D M 2 & S D M /C D M P o w er C on n ecto rs
J1
J3
+2 4 V U R
5
1
Fro m
To
+1 2 V U R
4
2
P.S .
SDM A
UR RET
J1/J2 3
3
1
PSM
J2
1
2
3
J3
+2 4 V U R
1
2 RET
N o te: J4 an d J5 a re no t o n
S D M /C D M
To
S D M 9 -J1 0
J4
+2 4 V U R
1
+1 2 V U R
2
3 UR RET
J4
1
2
To
SDM C
3
FC M
J5
1
2
3 2 0 11
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
To
SDM B
Part Number 9140300 − August 1998
To
SDM D
3
2 - 69
5#7URXEOHVKRRWLQJ • IP-20 Shear Valve Position Fault
IP-20 Shear Valve Position Fault (Page 1 of 2)
Start
- Check +24V
- Verify +24V UR (28.9V37.7V) (VP-1).
Fuse (Bottom
Fuse).
- Remove, inspect, and clean
Shear Valve (VP-48).
NO
- Replace Fuse if
necessary (PL-1).
Note: If the +24 UR
is approximately
the same as the
+12 UR, the +24V
Fuse is blown or
the +24V Power
Supply is defective.
28.9V-37.7V
?
From Main Menu, press
28.9V-37.7V
NO
„ DIAGNOSTICS
„ MORE
„ INITIALIZATION
?
YES
YES
- Verify +24V between Pins 1
and 2 of Shear Valve Driver
Board. If not present re-seat
cable.
- Re-seat cable from FCM-J8
to SVDM-J2.
?
- Lubricate Shear Valve Driver
(VP-42).
YES
- Replace Shear Valve Driver
Assy. (PL-9.1).
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
- Check Fuse again.
- Verify proper operation.
- Replace cable. (PL-1).
Problem
Corrected
NO
Fuse
Blown
Total Call
- Replace FCM (PL-14.1).
YES
?
NO
1
- Replace Power Supply
(PL-1).
Part Number 9140300 − August 1998
2 - 70
5#7URXEOHVKRRWLQJ • IP-20 Shear Valve Position Fault
IP-20 Shear Valve Position Fault (Page 2 of 2)
IP-20 Shear Valve Position Fault, 2 of 2
1
- If the Fuse continues to blow,
assemblies on the +12V UR or
+24V UR must be
disconnected one at a time
until the shorted assembly is
found and replaced. (See
Figures)
- Power-down instrument and
connect Meter between TP2+12V or TP1-+24V and TP4 on
PSM and remove connectors
until short is found.
- Replace defective assembly and
verify proper operation.
To
C D M 2-J3
FC M -J5
To
S D M /C D M -J3
J1
UR RET
1
3
4 +1 2 V U R
5 +2 4 V U R
J2
UR RET
1
3
4 +1 2 V U R
+2 4 V U R
5
B a ck
C D M 2 & S DM /C D M P o w er C on n ecto rs
J1
J3
+2 4 V U R
5
1
Fro m
+1 2 V U R
To
2
P.S . 4
SDM A
UR RET
J1/J2 3
3
1
PSM
J2
1
2
3
J3
To
S h ea r
Valve
D rive r-J3
1
2
+2 4 V U R
RET
N o te: J4 and J5 a re no t o n
S D M /C D M
To
S D M 9 -J1 0
J4
+2 4 V U R
1
+1 2 V U R
2
3 UR RET
J4
1
2
To
SDM C
3
FC M
J5
1
2
3 2 0 11
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
To
SDM B
Part Number 9140300 − August 1998
To
SDM D
3
2 - 71
5#7URXEOHVKRRWLQJ • IP-21 Flags
IP-21 Flags (Page 1 of 2)
Start
Problem
Corrected
- Ensure Customer has verified
an Instrument problem and
not a Specimen Specific
problem with multiple Manual
Differential Counts.
YES
?
Total Call
NO
- Clean Fan Filters and verify
all Fans are running properly.
- Ensure there is at least 6" of
clearance behind and on the
left side of Instrument.
- Perform Autoclean
- Ensure Instrument is not
subject to drastic temperature
variations (direct sunlight,
heating/air conditioning
ducts).
„ MORE
- Initialize and Prime
Instrument
- If Fan Filters were clogged
and then cleaned, allow a 15
minute temperature
stabilization period.
- Replace RBC Diluent (RBC/
PLT Flags) or WBC Lyse
(WBC Flags) Container.
- Verify Background
Counts (VP-56).
From Main Menu, press
„ SPECIAL PROTOCOLS
YES
„ AUTOCLEAN
- Clean Shear Valve (VP-48).
- Manually clean, debubble, and wet
Optical Flow Cell (VP-44), (VP45).
NO
Backgrounds
OK
- Run a Normal Patient
Sample exhibiting flags 5
times and print the
results.
- Observe the results and
refer the the System
Flags Table.
?
- Clean Optics Bench (VP-43).
1
- Replace In-line Sheath Filter (PL-4.2).
NO
Go to (IP-8).
YES
Problem
Corrected
?
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 72
5#7URXEOHVKRRWLQJ • IP-21 Flags
IP-21 Flags (Page 2 of 2)
1
Flagging
Parameters
WBC Flags Only
All Flagging
?
RBC and/or PLT Flags Only
- Verify Pressure 2 (VP-16) and
proper Sheath flow to Optical
Flow Cell. Troubleshoot as
necessary.
- From Main Menu, press
- From Main Menu, press
„ DIAGNOSTICS
„ DIAGNOSTICS
„ F12
„ F12
- Replace Optical Flow Cell
(PL-10).
„ F1
„ F1
- Replace MAM (PL-12).
„ SET POINT ENTRY
„ SET POINT ENTRY
- Replace SPM (PL-10).
- Verify WBC Threshold is 360.
- Verify RBC/PLT Threshold is 550.
- Replace CPU/DCM (PL-13).
- Ensure WBC Mixing Chamber is
being rinsed and drained properly.
- Ensure RBC/PLT Mixing Chamber is
being rinsed and drained properly.
- Verify/adjust WBC Gain (VP-23).
- Verify 0 o and 10 o Log Amplifiers (VP-28).
- Perform WBC OPTI-CAL
Verification/Adjustment (VP-24).
- Verify 0 o and 10 o Gain (VP-26), (VP-27).
- Replace MAM (PL-12).
Var Lym
LRI
- Replace MAM (PL-12).
- Replace SPM (PL-10).
Band
URI
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).
IG
LURI
- Replace CPU/DCM (PL-13).
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
System Flags Table
WBC Flags PLT Flags
RBC Flags
RBC Morph
Blast
Part Number 9140300 − August 1998
2 - 73
5#7URXEOHVKRRWLQJ • IP-22 External Waste Full
IP-22 External Waste Full (Page 1 of 2)
Start
System Uses
Waste Sensor Cable
NO
- Ensure Dummy Plug is
installed
- Reseat Dummy Plug
3 2 01 2
?
Problem
Corrected
- Empty Waste Container
YES
Total Call
?
- Reseat Waste Sensor Plug
- Clear Fault
NO
- Insert Insulated Tool into
Sensor Jack to open
contacts. See Figure
NO
Problem
Corrected
?
Total Call
1
YES
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 74
5#7URXEOHVKRRWLQJ • IP-22 External Waste Full
IP-22 External Waste Full (Page 2 of 2)
1
Problem
Corrected
YES
?
NO
- Disconnect Sensor Cable from Waste
Container and Sensor Jack and verify
infinite resistance between Fluid
Contacts. Replace as necessary (PL-3.1).
Problem
Corrected
YES
Total Call
?
Perform in the following order until problem is
corrected:
- Reseat CDM2, J7 and FCM, J2
- Replace Waste Sensor Jack (PL-3.2)
- Replace CDM2 (PL-16)
- Replace FCM (PL-14.1)
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
NO
Part Number 9140300 − August 1998
2 - 75
5#7URXEOHVKRRWLQJ • IP-23 Blank Display (Page 1 of 1)
IP-23 Blank Display (Page 1 of 1)
Start
- Connect Monitor to
Laptop. See Figure
- Turn Brightness and Contrast
controls fully clockwise.
- Open Computer Door to
observe 7-segment LED on
CPU/DCM.
- Verify Laptop
information displays on
Monitor.
- Verify Monitor Power and Power
Cord.
- With Power off, check Video
Cable, re-seat all Computer
Connectors, re-seat VGA Board.
- Cycle Analyzer Power and
verify the following:
- Stepper Motors all home.
NO
- Valves activate.
- 7-segment LED increments
until "E" and "1" are alternately
displayed.
Information
Displays
YES
- Analyzer "beeps" after
Initialization.
?
Problem
Corrected
YES
?
Total Call
YES
Data Station CRT
- Replace VGA Board (PL17.2).
NO
Initialization
OK
?
- Replace Monitor (PL17.3).
NO
- Troubleshoot
"dead" Analyzer
problem.
Attach Video Cable
From CRT to Laptop
External Monitor Port
Laptop
Computer
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
32101
Part Number 9140300 − August 1998
2 - 76
5#7URXEOHVKRRWLQJ • IP-24 HSSL Failures/Message Reception Time-Out (Page 1 of 1)
IP-24 HSSL Failures/Message Reception Time-Out (Page 1 of 1)
Start
- In order replace the
following.
- With Power OFF, check
and re-seat connectors on
the CPU/DCM, and Main
Computer.
- HSSL Cable (PL-17.3)
- CPU/DCM (PL-13)
- Serial Link/Keyboard
Controller (PL-17.2)
- Re-seat the HSSL Board.
- Check and re-connect the
HSSL Cable.
NO
- Reload HSSL Com (VP-67)
Problem
Corrected
?
YES
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 77
5#7URXEOHVKRRWLQJ • IP-25 Hard Drive Errors (Page 1 of 1)
IP-25 Hard Drive Errors (Page 1 of 1)
Start
- Format the Hard Drive (VP-61).
- Install Software (VP-53).
- Load System Setup (VP-65), (VP63).
- With Power OFF, re-seat
cable between Hard Drive
and Motherboard.
- Re-seat Power Cable on
Hard Drive and CDM.
NO
Problem
Corrected
NO
?
Problem
Corrected
YES
?
Total Call
- Replace Hard Drive (PL-17.1).
YES
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 78
5#7URXEOHVKRRWLQJ • IP-26 Printer Errors
IP-26 Printer Errors (Page 1 of 2)
Start
- Check power and power
cable.
- Ensure Printer Power is
ON, and Printer is Online.
- Ensure paper is loaded
properly.
Is Printer
Working
- Press On-line Button.
YES
NO
?
Clear Printer buffer.
NO
- From Main Menu, press
YES
Printer
On-Line
Printer
On-Line
?
?
NO
YES
- Replace Printer.
„ SETUP
„ CUSTOMIZE
REPORT
Total Call
„ STOP PRINTING
„ CONFIRM STOP
Clear Printer buffer.
- From Main Menu, press
„ SETUP
„ CUSTOMIZE
REPORT
Printer
Reset &
Print
?
NO
1
„ STOP PRINTING
„ CONFIRM STOP
YES
Total Call
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 79
5#7URXEOHVKRRWLQJ • IP-26 Printer Errors
IP-26 Printer Errors (Page 2 of 2)
1
- Connect Laptop Printer to
Printer Connector to
verify problem.
- Try printing with alternate
Printer Cable.
- Replace I/O Board (PL17.2).
NO
NO
Prints
OK
- Replace Motherboard
(PL-17.2).
Laptop
Printer
Prints
?
?
YES
YES
- Replace Printer.
- Replace Printer Cable
(PL-17.3).
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2 - 80
5#7URXEOHVKRRWLQJ • IP-27 Sample Loader & Aspiration Tower Error Messages
IP-27 Sample Loader & Aspiration Tower Error Messages
Error Message
Associated VP(s)
Associated Components
Parts List(s)
Mix Head stuck in angle position
VP-36
Stepper Motor, Home Sensor, SHM 2
PL-18.2, PL-18.1
Mix Head stuck in vertical position
VP-36
Stepper Motor, Home Sensor, SHM 2
PL-18.2, PL-18.1
VP-35
Up/Down Air Cylinder, Sensor, Valve 3,
SHM 2
PL-18.1, PL-18.2,
PL-18.3
VP-35
Up/Down Air Cylinder, Sensor, Valve 3,
SHM 2
PL-18.1, PL-18.2,
PL-18.3
Load zone empty detector malfunction
VP-.39
Sensor, SHM 2
PL-18.2, PL-18.3
Unload zone full detector malfunction
VP-39
Sensor, SHM 2
PL-18.2, PL-18.3
Unload zone nearly full detector
malfunction
VP-39
Mix Head not at top position
Mix Head stuck at top position
Tube stuck in position 3
Probe stuck at home position
Probe unable to reach home position
Invalid tube height
Unload area hardware malfunction
Tube dropped during mixing
PL-18.2, PL-18.3
VP-38
Tube Sensor Board, Rack Advance
Cylinder, Valve 1, SHM 2
PL-18.2, PL-18.3
VP-31
Aspiration Tower, Home Sensor, Needle
SHM 1
PL-6.1, PL-8
VP-31
Aspiration Tower, Home Sensor, Needle
SHM 1
PL-6.1, PL-8
VP-34
Aspiration Tower, Sensors S1/S2, SHM 1
PL-6.1, PL-8
Unload Side Sensors, SHM 2
PL-18.2, PL-18.3
Mixer Head, Valve 4, Regulator, SHM 2
PL-18.1, PL-18.2,
PL-18.3
Rack Index Cylinder, Valve 1, SHM 2
PL-18.1, PL-18.3
VP-38, VP-39
VP-37
Mix Zone Rack position error
VP-38
Unexpected tube in position 4 after
Rack advance
VP-38
Tube lost moving from position 3 to
position 4
VP-38
Rack barcode read failure
VP-40
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Sensor, SHM 2
Tube Sensor Board, SHM 2
Tube Sensor Board, SHM 2
PL-18.1, PL-18.2
PL-18.1, PL-18.2
Rack Barcode Label
Part Number 9140300 − August 1998
2 - 81
5#7URXEOHVKRRWLQJ • IP-27 Sample Loader & Aspiration Tower Error Messages
IP-27 Sample Loader & Aspiration Tower Error Messages
Error Message
Tube position barcode read failure
Barcode reader failure
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Associated VP(s)
VP-40
VP-33, VP-11
Associated Components
Parts List(s)
Rack Position Barcode Labels
Barcode Reader, Aspiration Tower, SHM 2
Part Number 9140300 − August 1998
PL-18.2, PL-6.1, PL-18.1
2 - 82
$SSHQGL[#5$#0#&DEOH#&RQQHFWLRQ#'LDJUDP • Table of Contents
$SSHQGL[#5$#0#&DEOH#&RQQHFWLRQ#'LDJUDP
Table of Contents
Figure 2A-1
Cable Connection Diagram, SL . . . . . . . . . . . . 2
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2A - 1
$SSHQGL[#5$#0#&DEOH#&RQQHFWLRQ#'LDJUDP
Figure 2A-1 Cable Connection Diagram, SL
9601320
FCM
SOL
1
2
3
4
5
(SOL 11-18)
SDM 1
SOL
9600930
NC
J1
J6
7
J2
J7
J3
J8
8
J4
J9
9520830
J5
J10
1
2
3
4
5
9520830
4
J56
J57
J58
J3
J1
SPEAKER &
STATUS BD
J2
SP
8
P/O
SDM/CDM
(SOL 41)
J61
J68
J67
1
J2
3
4
J3
J4
J15
J17
5
J5
J12
12"VAC OVFL
9520528
8"VAC OVFL
9520833
TEST CONN.
9520879
PRES PUMP
VAC PUMP
1
2
3
4
5
SDM 5
9600930
J6
J1
6
J2
J7
7
J3
J8
NC
J4
J9
9520859
J5
J10
9520860
NC
NC
9520851
J23
J24
9520861
9520866
9520849
PERIPHERAL BUS
J2
J5
J11
J10
(SOL 61-68)
1
2
3
4
5
SDM 6
9600930
J6
J1
6
J2
J7
7
J3
J8
8
J4
J9
J5
J10 9320860
1
2
3
4
5
J18
J8
9520385
HGB
J2
J3
NC
LASER
9520869
LASER P/S
9520896
12V, 24V UNREG.
J4
J15
NC
NC
J5
J3
9520853
J10
NC
NC
J7
J6
J12
J6
J7
9520001
FAN
J3
J8
9520620
FAN
J4
J13
9520829
J8
J1
J7
6
J13
J14
9520002
PWR SW
7
J9
8
J4
9520861
9520897
9520898
J6
9520789
J6
J7
J2
9520782
9520865
9520543
9520845
9520844
J2
MOTOR
A CORE SYR.
J1
J2
MOTOR
E PROBE
J1
J2
MOTOR
G SAMPLE PUMP
J1
9520155
9520842
J2
J10
NC
9520894
J3
SENSOR & CHOPPER DRIVER
3X 9600420
J12
NC
J13
NC
NC
J2
J13
J5
J14
9520892
1
SOLENOID
CHAIN CABLE
CHAIN CABLE
FLOW PANEL (X2)
PCS PANEL
LASER PANEL
J1
J2
MOTOR
C WBC LYSE SYR.
J1
J2
MOTOR
D RBC/HGB DIL SYR.
CHOPPER DRIVER
3X 9600420
J1
TO SL VAL-1
J2
TO SL VAL-2
J3
TO SL VAL-3
J4
TO SL VAL-4
J5
TO SL VAL-5
J10
TO SL MIXER SENS.
J12
9520883
9520882
9520780
TO TOWER MOTOR
J13
TO TOWER DC-MOTOR
J15
TO COVER SENSOR
J9
J11
J16
ASPIRATION
TOWER
CONTROLER
Y-VALVE ASSY
BOTTOM VIEW
MOTOR
B HGB LYSE SYR.
9601910
SHM(2)
J12
TO PC MOTHERBOARD
J2
NC
9601910
SHM(1)
9520884
J1
TO TOWER SOL.
9520872 TO TOWER SENS.
J10
9520894
J1
9520154
9520563
J14
9520895
9520612
PMT (CH4)
MPM SERIAL LINK
9520847
9520895
1
SDM1-4.....FLOW PANEL
SDM5,6.....REAGENT PANEL
J1
J2
J3
PMT PREAMP
9601010
9520856
J9
J11
+/-15V
32054
*S/L=SERIAL LINK
9520855
J8
NC
J9
9520871
9601000
PREAMP 10 DEG (CH2)
PMT PREAMP
9601010
J1
PMT (CH3)
J2
J3
J11
J12
2
J1
9520543
9601000
PREAMP 0 DEG (CH1)
J1
J10
J13
MOTOR
J4
J5
J3
J4
J1
MPM
9600920
J501
9520896
SPARE
9520782
9520854
J11
NC
J2
J1
J5
J7
J4
9520506
MAM
9601950
J2
BACK
9520869
9520866
9520828
12V, 24V UNREG.
J6
J3
NC
ADC2
J3
9520866
9520871
MUX DAC CTRL.
J1
J1
J5
9520841
TO REAGENT
RESERVOIRS
MDM 9601940
J2
9520789
9520841
TOP
9212064
}
FLOPPY
9520795
9520848
NC
9340008
LINEAR P/S
9212057
NC
HARD DISK
9520796
9520846
J5
J7
J6
J2
J1
PDM
9601980
(SOL 91-98)
SDM 9
9600930
J1
J6
J2
J7
J3
J8
J4
J9
J5
J10
NC
9520893
J4
J1
9520865
9520878
DIL SENS
J8
NC 9520835
J9
SHEATH SENS
J10
9520836
J3
J9
SPM
9601830
9520865
J3
9520892
DILUENT
9520839
P OVER PRESS
SENSOR
SHEAR VALVE DR.
9600900
EX WASTE
9520857
ADC3
J4
9520878
CDM 2
9601080
J7
J4
DAC3
J13
J16
DATA STATION *S/L
9520850
J17
J3
9520841
J19
9520864
9520865
9520500
J3
9520846
J11
9520505
J10
J12
J1
J2
J6 J18
J1
J2 PUMP
J4
J3 RELAY
9601200
J8
J7
NC
J20
J5
9520862
J1
DAC1
ADC1
9520852
ULTRA SONIC
SAMPLE DET
J4
J11
J16
J6
J14
J21
9520831
9520832
J10
9520870
J11
9520829
9520831
J13
9520832
J14
J16
J5
9600910 IN-LINE
REAGENT
SENSOR BD
J14
J1
9601161
2nd SAMPLE DET.
9520841
9520849
9520841
9520849
9520829
J6
J15
9520862
9520826
9520859
J22
J2
9520820
J1
2
9520879
(SOL 51-58)
J7
J25
VAC/PRES
9601220
START SW
J13
J26
9520838
9520834
1
J1
J12
9520826
NC
SOLENOID
6
7
9520828
J9
CPU
9601820
J6
9520825
NC
9520825
9520847
9520783
+5V. +/-15V
EXT. CPU BUS
2
3
J51
J52
J53
J54
J6
P/O
SDM/CDM
(SOL 31-38)
1
J3
NC
J9
9520858
(SOL 21-28)
SDM 2
9600930
J1
J6
6
J2
J7
7
J3
J8
8
J4
J9
9520858
J5
J10
9520837
SDM DATA, +5V
9520893
9520890
9520888
9520887
9212106
TO SL MIXER MOTOR
TO BAR CODE READER
TO SL RACK SENSORS
TO SL TUBE SENS ASSY
J16
SAMPLE LOADER
CONTROLLER
4PLCS
9520255 ASY3A
CONNECT THE DOOR AND PLATE TO CHASSIS GND.
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2A - 2
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Table of Contents
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV
Table of Contents
Figure 2B-1
Figure 2B-2
Figure 2B-3
Figure 2B-4
Figure 2B-5
Figure 2B-6
Figure 2B-7
Figure 2B-8
Figure 2B-9
Figure 2B-10
Figure 2B-11
Figure 2B-12
Figure 2B-13
Figure 2B-14
Figure 2B-15
Figure 2B-16
Figure 2B-17
Figure 2B-18
Figure 2B-19
Figure 2B-20
ADC Block Diagram . . . . . . . . . . . . . . . . . . . . . 2
ADC Block Diagram . . . . . . . . . . . . . . . . . . . . . 3
DAC Block Diagram . . . . . . . . . . . . . . . . . . . . . 4
Measurement & Subsystem Block Diagram . . 5
Solenoid Driver Modules 1, 2 & SDM/CDM . . . 6
Solenoid Driver Modules 5, 6, 9. . . . . . . . . . . . 7
Motor Driver Block Diagram. . . . . . . . . . . . . . . 8
CS Sensor Interface Block Diagram . . . . . . . . 9
S/L Sensor Interface Block Diagram . . . . . . . 10
Vacuum/Pressure Block Diagram . . . . . . . . . 11
Power Distribution Block Diagram . . . . . . . . . 12
Computer Block Diagram. . . . . . . . . . . . . . . . 13
Measurement Block Diagram. . . . . . . . . . . . . 14
Solenoid Control Block Diagram . . . . . . . . . . 15
Motor Control Block Diagram. . . . . . . . . . . . . 16
Sensor Block Diagram . . . . . . . . . . . . . . . . . . 17
Power Distribution Block Diagram . . . . . . . . . 18
Normal 10, 90 and 0, 90D Scattergrams . . . . 19
Normal 10, 90 and 0, 90D Scattergrams . . . . 20
Normal PLT and RBC Histograms . . . . . . . . . 21
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 1
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-1 ADC Block Diagram
Figure 2B-1 Figure 2B-1ADC Block Diagram
Display
PC Module
VPM
High Speed
Serial Link
Tag Bus
SPM
High
Speed
External
CPU
Bus
FCM
MAM
CPU/DCM
MUX DAC Control Bus
MPM
Serial
Bus
MPM
Peripheral Bus
Serial RS-485 Bus To SHM1
(Aspiration Tower Control)
32043
Serial RS-485 Bus To SHM2
(Sample Loader Control)
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 2
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-2 ADC Block Diagram
Figure 2B-2 ADC Block Diagram
Display
PC Module
High Speed
Serial Link
ADC1
A/D
MUX
ADC3
ADC2
Tag Bus
CPU/DCM
ADC4
MAM
SPM
FCM
MUX DAC Control
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
VPM
32044
2B - 3
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-3 DAC Block Diagram
Figure 2B-3 DAC Block Diagram
Display
PC Module
High Speed
Serial Link
DAC1
D/A
DAC2
DAC3
CPU/DCM
DAC4
MAM
SPM
FCM
MUX DAC Control
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
VPM
32045
2B - 4
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-4 Measurement & Subsystem
Figure 2B-4 Measurement & Subsystem Block Diagram
Display
PD
0 o PAM
0 o Lin
10 o Lin
PD
10 o PAM
90 o Lin
90 o D Lin
PMT
90 o PAM
MAM
PC Module
0 o Log
10 o Log
High Speed
Serial Link
SPM
Analog Out
VDYN
Tag Bus
PMT
90 o D PAM
CPU/DCM
VDYN
External CPU Bus
HGB
Flow
Cell
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Peripheral Bus
LED Current
HGB Signal
HGB Voltage
FCM
32046
Part Number 9140300 − August 1998
2B - 5
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-5 Solen Dr Mod 1, 2 & SDM/CDM
Figure 2B-5 Solenoid Driver Modules 1, 2 & SDM/CDM
Solenoid Data
Bus
3-1 WC2 Vacuum Supply (5" Hg)
3-2 WC2 Pressure (12 psi)
3-3 RBC Diluent Syringe Supply
3-4 CS Probe Diluent Flush (NC)
CLK
3-6 CS Vent Trap Drain (WC3)
SDM/CDM
3-7 CS Probe Drain (WC4)
(CDM1)
3-8 CS Vent Trap Vent
4-1 NOC Sample Staging
4-7 ON/OFF
4-8 CW/CCW
Motor
Drive
Module
DC
Y-Valve
Motor
1-1 Shear Valve Diluent
Flush
1-2 Sample Aspiration Vacuum
FCM
1-3 Shear Valve Drain (WC4)
CLK
1-4 Open Sample Probe Drain (WC4)
S D M
1
1-5 Open Sample Probe Diluent Flush (NC)
1-6 Spare
1-7 WC4 Vacuum Supply (12" Hg)
1-8 WC4 Pressure Supply (12 psi)
Peripheral Bus
2-1 RBC Mixing Chamber Drain
2-2 Sample Injection Syringe Sheath Supply
CPU/
DCM
2-3 Main WBC Lyse Supply (NC)
2-4 HGB Lyse Syringe Output
SDM 2
CLK
2-5 WBC Lyse Syringe Output
2-6 Diluent Syringe HGB Dilution
2-7 Diluent Syringe RBC Dilution
2-8 Main HGB Lyse Supply (NC)
1 12 psi Accumulator Vent
2 9 psi Pressure Supply
VPM
3 5 psi Pressure Supply
4 12" Hg Accumulator Vent
5 Vacuum #2 Vacuum Supply
32047
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 6
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-6 Solenoid Dr Modules 5, 6, 9
Figure 2B-6 Solenoid Driver Modules 5, 6, 9
Solenoid Data Bus
FCM
CLK
SDM 5
5-1
5-2
5-3
5-4
5-5
5-6
5-7
5-8
HGB Flow Cell Vent
Staging Pump Input
WBC Cup Vent
RBC/PLT Sample Staging
WBC Sample Staging
Optical Flow Cell Output
Optical Flow Cell Drain
Spare
SDM 6
6-1
6-2
6-3
6-4
6-5
6-6
6-7
6-8
Sheath Reservoir Vacuum (12" Hg)
Diluent Reservoir Vacuum (12" Hg)
Diluent Reservoir Pressure (5 psi)
Sheath Reservoir Pressure (9 psi)
Sheath Reservoir Output (NC)
Diluent Reservoir Output (NC)
WC1 Pressure (12 psi)
WC1 Vent
SDM 9
9-1
9-2
9-3
9-4
9-5
9-6
9-7
9-8
Optical Flow Cell Sheath Flow
WBC Cup Drain (WC3)
HGB Flow Cell Drain (WC3)
HGB Flow Cell Diluent Flush
RBC Mixing Chamber Diluent Flush
WBC Mixing Chamber Diluent Flush
WC4 Vacuum (12" Hg)
WC4 Pressure (12 psi)
CDM 2
Peripheral
Bus
CPU/
DCM
CLK
CLK
32048
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 7
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-7 Motor Driver Block Diagram
Figure 2B-7 Motor Driver Block Diagram
Aspiration Probe Up/Down
A
SHM1
DC Barcode Spin
RS-485
Serial
Bus
MPM
Serial
Bus
MPM
CPU/DCM
Driver
Motor
A
A
Sample Delivery Syringe
B
B
HGB Lyse Syringe
C
C
WBC Lyse Syringe
D
D
RBC Diluent Syringe
E
E
Open Probe Up/Down
G
G
Sample Staging Pump
AC Vacuum Pump
Peripheral Bus
VPM
AC Pressure Pump
Limit Switch (CW)
Shear
Valve
Driver
FCM
4-7
SCM/CDM
4-8
DC Shear Valve Motor
Limit Switch (CCW)
ON/OFF
CW/CCW
Motor
Drive
Module
DC
Y-Valve
Motor
32049
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 8
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-8 CS Sensor Interface Block
Figure 2B-8 CS Sensor Interface Block Diagram
Display
Vacuum Accumulator #1 Wet
VPM
Vacuum Accumulator #2 Wet
PC Module
High Speed
Serial Link
Diluent Empty
WBC Lyse Empty
HGB Lyse Empty
CPU/DCM
Peripheral Bus
External Waste Full
CDM2
RS-485
Serial
Bus
Diluent Reservior Full
Sheath Reservoir Full
FCM
Tube Height (S1)
Tube Height (S2)
SHM1
Shear
Valve
Driver
GS1 Home
Door Closed
Sample Cover
Interface
Board
Limit Switch (CCW)
Limit Switch (CW)
Ultrasonic Short Sample
Y-Valve Closed Mode Sensor
Short Sample
Interface
Board
MDM
Y-Valve Open Mode Sensor
32050
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 9
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-9 S/L Sensor Interface Block
Figure 2B-9 S/L Sensor Interface Block Diagram
Third Tube Sensor
Typical Tube Sensor
Fourth Tube Sensor
J11
25 KHZ LED Drive
RS-485 Bus
To CPU/DCM
Power
From PDM
Tube
J16
SHM2
J12
Load Side Empty Sensor
J9
U. S. Fourth Rack Sensor
U. S. Fifth Rack Sensor
Mixer Home Sensor (Up)
J10
Mixer Head Home Sensor
32105
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 10
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-10 Vacuum/Pressure Block
Figure 2B-10 Vacuum/Pressure Block Diagram
Vacuum/Pressure Module
32051
ADC4
Analog Multiplexer
Multiplexer
&
Sample and Hold Circuitry
Threshold Setpoints
DAC4
P1
SPP1
Pres #1
Regulator
12 psi
P2
SPP2
P1 P2 P3 V1 V2
P3
V1
SPP3
Pres #2
Regulator
9 psi
V2
SPV1
Pres #3
Regulator
5 psi
SPV2
Vac #1
Regulator
12" Hg
V3
Vac #2
Regulator
(Variable)
V5
Relay PCB
Relay PCB
V2
Pressure
Pump
Pres #1
Accumulator
12 psi
Pres #2
Accumulator
9 psi
Pres #3
Accumulator
5 psi
Vacuum
Pump
V1
V4
Vent
Vent
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Vac #1
Accumulator
12" Hg
Part Number 9140300 − August 1998
Vac #2
Accumulator
5" Hg
(Varies)
2B - 11
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-11 Power Distribution Block
Figure 2B-11 Power Distribution Block Diagram
AC Line
Pow er Sw itch
MAIN POWER SUPPLY
PDM J2
J3
J13
+ 5, GND-PC MB
+ 12, -12, GND, PF-PC MB
+ 5, -15, GND-MAM, SPM, CPU
J4
+ 5, + 24, GND-MDM
+ 5V
J5
Filter
(CORCOM)
J5
+ 12V
-12V
+ 24V
GND
+ 5, + 12, -12, + 24-MPM
J1
J6
J7
Spare
+ 5, + 15, -15, GND-VPM, FCM
J8
Fuse
J4
(Rear)
+ 15V
-15V
J10
J9
GND
AC Voltage
Selector
J11
J12
J13
+ 5. + 12, GND-Hard Drive
+ 5. + 12, GND-Floppy Drive
+ 5, + 15, -15, GND-SHM1
+ 5, + 15, -15, GND-SHM2
Transformer
J4
(Top)
115VA C
LASER P/S
+ 2400VDC
LASER
115VAC, Rear Fan
J7
115VAC, Side Fan
J8
115VAC, PRM
J2
32052
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 12
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-12 Computer Block Diagram
Figure 2B-12 Computer Block Diagram
H ar d D i s k
Flo p p y D is k
D r iv e
D ri v e
C PU / D C M
K e y b o ard
HSSL
M o t h er b o a rd
Pa r a lle l/
S e ria l Po rt s
H SS L
C o n t r o ller
Pa r a lle l/
S e ria l Po rt s
Bu s
V id e o D is p la y
C o n t r o ller
32053
M e m b r a n e K e y b o a rd
Po w er
V id e o D is p la y
S u p p ly
M o d u le
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
C RT
Part Number 9140300 − August 1998
2B - 13
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-13 Measurement Block Diagram
Figure 2B-13 Measurement Block Diagram
TP1
TP3
J10
6
Amplifiers
CH 1 Lin
CH 2 Lin
CH 3 Lin
CH 4 Lin
CH 1 Log
CH 2 Log
Lin Amplifiers
4
TP2
0 Photodiode Preamp
SPM
MAM
J1
TP8
2
Log Amplifiers
J15
J6
1
3
5
7
9
11
1
3
5
7
9
11
CH 1 Lin
CH 2 Lin
CH 3 Lin
CH 4 Lin
CH 1 Log
CH 2 Log
J7
Peak
Hold
Circuits
Amplifier
MUX
1
ADC3
2 To CPU/DCM
3
AGND
AGND
TP1
Amplifiers
AGND
J1
TP4
J12
6
Lin Amplifiers
4
FCM
TP2
10 Photodiode Preamp
TP9
2
J18
HGB Voltage
TP7 TP1
Log Amplifiers
HGB
Flow
Cell
AGND
AGND
LED Current
Amplifier
1
2
3
4
5
J25
MUX
Amplifier
1
ADC1
2 To CPU/DCM
3
AGND
TP2
Amplifiers
J2
TP5
J13
4
4
AGND
AGND
J24
Lin Amplifiers
1
TP3
PMT
2
VDYN/-100
AGND
Amplifier
8
ADC4
From
VPM
8
TP1
90 PMT Preamp
2
32055
AGND
TP2
Amplifiers
TP6
J14
4
4
8
8
Lin Amplifiers
TP3
PMT
VDYN/-100
AGND
J2
TP2
TP1
90 D PMT Preamp
2
+10V Reference
AGND
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 14
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-14 Solenoid Control Block
Figure 2B-14 Solenoid Control Block Diagram
CLK
J1
CLK
CLK
J2
WC4 Vacuum Supply (12" Hg)
WC4 Pressure (12 psi)
RBC Diluent Syringe Supply
CS Probe Diluent Flush (NC)
CS Vent Trap Drain (WC3)
CS Probe Drain (WC4)
CS Vent Trap Vent
NOC Sample Staging
CLK
CLK
Peripheral Bus
J2
J1
ON/OFF
CW/CCW
J6
Y
Valve
Motor
J2
J1
J9 J2
J3
J4
J5
J6
J10 J7
J8
CDM2
J1
J2
1-1
1-2
1-3
1-4
1-5
1-6
1-7
1-8
Shear Valve Diluent Flush
Sample Aspiration Vacuum
Shear Valve Drain (WC4)
Open Sample Probe Drain (WC4)
Open Sample Probe Diluent Flush (NC)
Spare
WC2 Vacuum Supply (4" Hg)
WC2 Pressure Supply (12 psi)
2-1
2-2
2-3
2-4
2-5
2-6
2-7
2-8
RBC Mixing Chamber Drain
Sample Injection Syringe Sheath Supply
Main WBC Lyse Supply (NC)
HGB Lyse Syringe Output
WBC Lyse Syringe Output
Diluent Syringe HGB Dilution
Diluent Syringe RBC Dilution
Main HGB Lyse Supply (NC)
5-1
5-2
5-3
5-4
5-5
5-6
5-7
5-8
HGB Flow Cell Vent
Staging Pump Input
WBC Cup Vent
RBC/PLT Sample Staging
WBC Sample Staging
Optical Flow Cell Output
Optical Flow Cell Drain
Spare
J2
1
2
To
3
5 SDMB
6
7
J4
1
2
3
5
6
7
SDM6
J1
J9 J2
J3
J4
J5
J6
J10 J7
J8
J6
SDM1
SDM2
J1
J9 J2
J3
J4
J5
J6
J10 J7
J8
On CLK A
1
Hi CLK A
2
On CLK B
3
Hi CLK B
From 4
On CLK C
FCM 5
Hi CLK C
6 On CLK D
7
Hi CLK D
8
Reset
9
SDM5
DC
1
2
To
3
5 SDMA
6
7
J6
J1
J1
J9 J2
J3
J4
J5
J6
J10 J7
J8
J16
CPU/DCM
J17 J1
+24V UR
5
From 4 +12V UR
P. S. 3 UR RET
1
MDM
J68
J67
J4
J19
3-1
3-2
3-3
3-4
3-6
3-7
3-8
4-1
J6
FCM
J1
J3
J51
J52
J53
J54
J56
J9 J57
J58
J61
J3
CDM Connectors
SDM/CDM
Solenoid Data/+5V/5V Ret.
6-1
6-2
6-3
6-4
6-5
6-6
6-7
6-8
Sheath Reservoir Vacuum (12" Hg)
Diluent Reservoir Vacuum (12" Hg)
Diluent Reservoir Pressure (5 psi)
Sheath Reservoir Pressure (9 psi)
Sheath Reservoir Output (NC)
Diluent Reservoir Output (NC)
WC1 Pressure (12 psi)
WC1 Vent
9-1
9-2
9-3
9-4
9-5
9-6
9-7
9-8
Optical Flow Cell Sheath Flow
WBC Mixing Chamber Drain (WC3)
HGB Flow Cell Drain (WC3)
HGB Flow Cell Diluent Flush
RBC Mixing Chamber Diluent Flush
WBC Mixing Chamber Diluent Flush
WC3 Vacuum (12" Hg)
WC3 Pressure (12 psi)
To
SDMC
J5
1
2
To
3
5 SDMD
6
7
SDM9
J1
J9 J2
J3
J4
J5
J6
J10 J7
J8
32056
VPM
J17
RS-485 Bus
J1
J2
J3
J4
J5
SHM1
Aspiration Tower
J16 J1
J2
J3
J4
J5
J6
J7
J8
GS2 Stop
Door Lock
Spare
Spare
Spare
Spare
Spare
Spare
RS-485 Bus
J16
J1
J2
J3
J4
J5
J6
J7
J8
J1
SHM Solenoid Connector
+24V
J1-J8
On CLK 1
Hi CLK 1
On CLK 2
Hi CLK 2
On CLK 3
Hi CLK 3
On CLK 4
Hi CLK 4
Reset
1
J3
1
2
3
4
To
5
CDM1
6
7
8
9
15
PD0
PD1
PD2
PD3
PD4
PD5
PD6
PD7
+5V
1
2
3
4
To
5
SDMs
6
7
8
9
10
SDM Connectors & Driver Outputs
J9
PD0
1
PD1
2
PD2
3
PD3
4
PD4
5
PD5
6
PD6
7
PD7
8
+5V
9
10
+24VUR
Hi/Lo
+12VUR
Driver
TP1
2
AGND
On/Off
SHM2
FCM Connectors
1 12 psi Accumulator Vent
2 9 psi Pressure Supply
3 5 psi Pressure Supply
4 12" Hg Accumulator Vent
5 Vacuum #2 Vacuum Supply
Sample Loader
V1 Rack Advance
V2 Load Side Cross Transfer
V3 Mixer Lift
V4 Mixer Bladders
V5 Unload Side Cross Transfer
Spare
Spare
Spare
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Driver
On CLK 5
Hi CLK 5
On CLK 6
Hi CLK 6
Reset
J4
1
2
3
To
4 CDM2
9
15
+24VUR
+12VUR
UR RET
On CLK 9
Hi CLK 9
Reset
J1-J8
DGND
DGND
J2
1
2
To
3
5 SDM9
6
7
J10
+24VUR
1 +12VUR
2 UR RET
3
On CLK
5 Hi CLK
6
Reset
7
On/Off
TP2
Driver
Solenoid Drive
1
2
AGND
Part Number 9140300 − August 1998
2B - 15
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-15 Motor Control Block Diagram
Figure 2B-15 Motor Control Block Diagram
MPM Stepper Motor Control
Sample Loader Motor Control
Aspiration Tower Motor Control
J6
MPM Serial Link
J3
JI
JI
CDB
A
J2
Delivery Syringe
Mixer Stepper Motor
GS1 Stepper Motor
CPU/DCM
J4
JI
CDB
J2
B
HGB Lyse Syringe
PH0
J5
JI
CDB
J2
C
WBC Lyse Syringe
PH0
J6
JI
CDB
J2
D
RBC/HGB Diluent Syringe
1
PH0 1
Motor
MPM
JI
J7
CDB
J2
E
Open Probe
JI
CDB
J2
MPM Motor Control Block Diagram (Motor 0)
G
Sample Staging Pump
J3
Ramping
A/D
Converter
8
FB-
9
+5V
M0 PH0
M0 PH1
7
M0 PH1
J3
J3
TO
CDB
M0
Current
Control
Latch
DC
Shear Valve Motor Control
3
4
TO
CDB
5
6
Barcode Spin Motor
+24V 1
J8
Driver 2
2
M0 I1
M0 PH0
5
1
+24V
Digital Control Bus
M0
Phase
Control
Latch
PH1 4
PH1 5
J3-J14
TO
CDB
M0 I0
J1
MPM
Serial
Link
4
Typical MPM Motor Control Output Connector
FB+
Discharge
SHM2 J13
PH1
PH1
J9
Discharge
Complete
PH0 2
J13
SHM1
J8
Microprocessor
&
Digital Control
Motor
2
M0 I0
M0 I1
4
TO
CDB
5
M1-M11
Control
Latches
J4-J14
6
FCM
J8
7
1
+5V
1
3
DGND
3
4
5
FB+
J2
On/Off
4
CW/CCW
5
Shear
Valve
J4
Driver
Module
1
3
+ Drive
- Drive
DC
8
FB-
Shear Valve Motor
9
10
Y-Valve Motor Control
CDB Block Diagram
Chopper Driver Board
J1
PH0
J2
6
4
5
TO
MPM
7
2
Stepper
Motor
Phase 0
Driver
I0
I1
MA
PH0 1
MB
PH0 2
Motor
PH1
Stepper
Motor
Phase 1
Driver
+24V
3 +5V
MA
MB
PH1
J67 2
8
9
FB+
FB-
CW/CCW
FB-
2
DC
2 J1
4
5
Pump Relay Board
GND
Winding
Voltage
Feedback
(Diagnostics)
Y-Valve Motor
1
MDM J14
3+
FB+
2 J2
Vacuum & Pressure Pump Control
PH1
TP1
1
On/Off
J68 2
SDM/CDM
1
VPM
J18 3
4
+5V
Vac. Pump On
Pres. Pump On
4-
AC In 1
K1
AC Out 2
F1
Line
1
Neutral J4
2
115VAC In
Vacuum Pump
1
J1
1
J3
3
2
AC
4
3+
AC In 1
K2
4-
AC Out 2
32057
1
J2
2
AC
Pressure Pump
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 16
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-16 Sensor Block Diagram
Figure 2B-16 Sensor Block Diagram
Typical FCM & VPM Fluid Sensor Input
Reagent Empty In-Line Sensors
Aspiration Tower Sensors
IFSB
Electrode +
FLUID
LINE
Electrode -
E1
Diluent Empty
E2
WBC Lyse Empty
E4
HGB Lyse Empty
J1
E5
GS1 Home Sensor
1
1
2
2
4
4
+5V
Tube Height Sensor (S2)
E6
J26
E8
GND
6
Typical SHM Optical Sensor Input
3
Tube Height Sensor (S1)
J9-10
6 J10
9
Cover Sensor
3
Door Sensor
6
6
SHM1
Typical SHM Magnetic Sensor Input
J9
CDM2
Reagent & Waste Level Sensors
1
2
3
4
5
6
7
8
9
10
11
12
In
In
In
In
Sample Loader Sensors
External Waste Full
1 J7
10
10
Diluent Reservoir Level
1 J8
11
11
13
13
J2
J6
Sheath Reservoir Level
Load Side Empty Sensor
1 J10
GND
15
DGND
3
U. S. Fifth Rack Sensor
6 J9
U. S. Fouth Rack Sensor
9
Mixer Head Home Sensor
3
Typical SHM Tube Sensor Input
+5V
J11
SHM2
15
J10
Mixer Lifter Up Sensor
6
Second Sample Sensor Board
FCM
+5V
Fourth Tube Sensor
4
Third Tube Sensor
8
J11
TP4
TP2
1
+5V
1
2 J14
2
Ref
J1 3
AGND
3
Vacuum Accumulator Sensors
In
25KHZ LED Drive
In
AGND
Vacuum #1 Wet
1 J1
Vacuum #2 Wet
VPM
1 J2
AGND
25KHZ LED Drive
1
2
3
4
5
6
7
8
9
10
11
12
Y-Valve Sensors
Closed Mode Sensor
2 J4
Open Mode Sensor
2 J5
Ultrasonic Short Sample Sensor Board
MDM
1
+5V
1
J1 2
3
2 J11
AGND
Typical MDM Optical Sensor Input
3
+5V
Shear Valve Driver Module
Limit Sensor Baord
2
J1
3
4
+5V
Valve CCW
Valve CW
2
3
4
1
J1
TP1
3
J2
6
DGND
6
6
7
J4-J5
+5V
1
2
3
1
DGND
3
Valve CW
In
J8
6
Valve CCW
AGND
7
RBC Overpressure Sensor
NO
+5V
GND
2
J10
3
32058
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 17
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-17 Power Distribution Block
Figure 2B-17 Power Distribution Block Diagram
Main Power Supply Power Connectors
Power Distribution Module Power Connectors
J1
BACK
J501
PWR Fail
PWR Fail
1
2
-12V 3
+12V 4
+24V 5
GND 1 6
GND 1 7
+5V 8
+5V 9
J8
To
FCM-J21
VPM-J12
1
2
3
4
5
6
+5V
GND 2
TP8
+15V
GND 2
-15V
+15V
TP2
-12V
J9
Input
From
P.S.
J4
1
2
3
4
-15V
GND 2
KEY
+15V
TP7
+24V
PWR Fail
+5V
+12V
-12V
GND 1
GND 1
J10
To
PC
Hard
Disk
+12V
1 GND 2
2 GND 2
3 +5V
4
TP1
TP5
J11
To
PC
Floppy
Disk
1
2
3
4
+12V
GND 2
GND 2
+5V
GND 1
GND 2
GND 1
-15V
+24V
1
2 +5V
3
4 +15V
5 -15V
6 GND 2
7 GND 2
8
To
SHM 2-J12
To
PDM-J1
To
CDM2-J3
FCM-J5
J3
1
2
3
4
5
6
1
2
3
4
5
6
7
8
J5
+24V 1
+5V
2
GND 1 3
To PC
Motherboard
To
MAM-J3
SPM-J3
CPU/DCM-J14
To
SDM/CDM-J3
To
Shear
Valve
Driver-J3
To
PDM-J9
+5V
+24V
+5V
+15V
-15V
GND 2
TP3
J6
-12V
+12V
+24V
GND 1
GND 1
GND 1
+5V
1
2
3
4
5
6
7
8
From
P. S.
J1/J2
-12V
+12V
+24V
GND 1
GND 1
+5V
+5V
J2
J1
UR RET
1
3
4 +12V UR
5 +24V UR
1
To
2 SDMB
3
J2
UR RET
1
3
4 +12V UR
5 +24V UR
J4
1
2
3
J5
J4
1 -15V
2 GND 2
3 +15V
1
To
2 SDMD
3
FCM Power Connectors
To
PRM-J4
To
MPM-J2
To
Laser
Power
Supply
J7
TP4
+12V
Typical MPM Motor Control Output Connector
J3-J14
-12V 1
+12V 2
3
GND 1 4
GND 1 5
GND 1 6
7
+5V 8
J2
115VAC
1
2 Neutral
TOP
J4
115VAC
1 Neutral
2
To
Rear
Fan
J7
115VAC
1
2 Neutral
Spare
To
Side
Fan
J8
115VAC
1 Neutral
2
1
+24V
+5V
M0 I0
M0 I1
M0 PH0
M0 PH1
FB+
FB-
To
SDMC
J3
+24V UR
1
2 RET
-15V
GND 2
CDM 1 & 2 Power Connectors
J1
+24V UR
1
5
To
+12V UR
4
2 SDMA
UR RET
3
3
1
J3
To
MDM-J3
TP6
J13
1
2
3
4
5
6
7
8
To PC
Motherboard
J4
+5V
+5V
GND 1
GND 1
J12
To
SHM 1-J12
J2
GND 1
1
GND 1 2
+5V 3
4
+5V 5
+5V 6
Input
From
P.S.
J501
1
2
3
4
5
6
7
8
9
2
J3
To
SDMs-J9
To
SDM9-J10
To
Shear
Valve
Driver-J2
To
Ultrasonic
Sample
Sensor-J3
To
Second
Sample
Sensor-J1
9 +5V
10 GND
J4
+24V UR
1
2 +12V UR
3 UR RET
J8
1 +5V
3 GND
J11
1 +5V
3 GND
J14
1 +5V
3 GND
3
4
5
6
TO
CDB
Note: Pin configurations on the assemblies that are not
shown are the same as those shown.
32059
7
8
9
10
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 18
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-18 Normal 10,90 & 0,90D S'grams
Figure 2B-18 Normal 10, 90 and 0, 90D Scattergrams
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 19
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-19 Normal 10,90 & 0,90D S'grams
Figure 2B-19 Normal 10, 90 and 0, 90D Scattergrams
4 th Cut
3 rd Cut
N
M
E
E
L
0° Size
90° Dep
N
B
M
0°
B - Basophil
E - Eosinophil
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
B
L
10° Complexity
L - Lymphocyte
M - Monocyte
32103
N - Neutrophil
Part Number 9140300 − August 1998
2B - 20
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-20 Norm PLT & RBC Histograms
Figure 2B-20 Normal PLT and RBC Histograms
RBC/PLT
PLT
3
2
1
PLT
1. Curve Starts to the Right of the Lower Threshold.
RBC
2. Single Peak
32104
3. Good Distance From Lower Threshold at Peak
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2B - 21
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV • Table of Contents
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV
Table of Contents
Figure 2C-1 Flow Diagram, Complete . . . . . . . . . . . . . . . . . 2
Figure 2C-2 Sample Loader Flow Diagram . . . . . . . . . . . . . 3
Figure 2C-3 Vacuum/Pressure Flow Diagram . . . . . . . . . . . 4
Figure 2C-4 Reagent Flow Diagram . . . . . . . . . . . . . . . . . . 5
Figure 2C-5 Sample Aspiration Flow Diagram. . . . . . . . . . . 6
Figure 2C-6 Dilution and Mixing Flow Diagram . . . . . . . . . . 7
Figure 2C-7 Sample Staging Flow Diagram . . . . . . . . . . . . 8
Figure 2C-8 Sample Delivery Flow Diagram . . . . . . . . . . . . 9
Figure 2C-9 Waste Flow Diagram . . . . . . . . . . . . . . . . . . . 10
Figure 2C-10 Vent Flow Diagram . . . . . . . . . . . . . . . . . . . . 11
Service Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2C - 1
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV • Figure 2C-1 Flow Diagram, Complete
Figure 2C-1
Flow Diagram, Complete
REAGENT & WASTE PANEL
MUFFLER
BLOOD/IN
EMPTY
SENSORS
PRES
PUMP
62
63
61
64
HGB DIL/IN
FILTER
WBC LYSE
FRONT VALVE
1
DILUENT
RESERVOIR
REAR VALVE
HGB LYSE
SHEATH
RESERVOIR
28
23
PS1
TOP
HGB LYSE (BLU)
SENSOR
RBC DIL/IN, FARSIDE
RBC/OUT
TO AIR
TO AIR
WBC DIL/IN, FARSIDE
HGB/OUT
DILUENT
WBC LYSE
WASTE
TO AIR
BLOOD/OUT, FARSIDE
WBC/OUT
HGB LYSE
ACC 1
PRES 1
FILTER (SMALL)
WBC LYSE (VIO)
66
TO AIR
(12 psi)
DILUENT (RED)
FILTER
(LARGE)
2
WBC
FLOW
CELL
91
65
96
94
DILUENT (RED)
1 2 3 4 5
56
53
WBC OUT
PS2
ACC 2
PRES 2
(9 psi)
95
3
RBC
MIXING
CHAMBER
52
HGB
12 uL
RBC
1.67 uL
WBC
M. C.
51
HGB OUT
WBC
20 uL
MOTOR-X
BLOOD
SAMPLE
VALVE
VENT
ACC
22
MOTOR-G
RBC DIL
RBC OUT
11
12
41
MOTOR-A
VENT
Y-VALVE
47,48
TO SAMPLE LOADER
(SL MODEL ONLY)
21
VAC
14
15
PUMP
92
TO AIR
26
25
38
MOTOR-E
24
33
10 mL
RBC
DIL
SYRINGE
SY4
34
WC-4
TO AIR
VENT
TRAP
WC-3
SENSOR
37
ACC 4
DRAIN
31
32
5
PS5
97
CLOSED
MODE
PROBE
98
MOTOR-C
WC-2
2.5 mL
HGB
LYSE
SY2
2.5 mL
WBC
LYSE
SYRINGE
SY3
MOTOR-D
4
VAC 1
(12" Hg)
68
17
27
OPEN
SAMPLE
PROBE
67
93
PRESSURE
SWITCH
VAC 1 (GRN)
PS4
500 uL
SAMPLE
INJECTION
SY1
TRAP
TRAP
MUFFLER
TRAP
HGB
FLOW
CELL
ULTRASONIC
SENSOR
13
SENSOR
TO AIR
PERISTALTIC
PUMP
55
HGB DIL
PS3
ACC 3
PRES 3
(5 psi)
57
WBC LYSE
SAMPLE
SENSOR 2
SENSOR
54
MOTOR-B
WC-1
18
36
VAC 1
(GRN)
VAC 2 (BRN)
SENSOR
PRESS 1 (ORN)
ACC 5
VAC 2
(VAR.)
2 WAY PINCH VALVE NORMALLY OPEN
WASTE (BLK)
2 WAY PINCH VALVE NORMALLY CLOSED
DRAIN
VAC 2 (BRN)
AIR FLOW RESTRICTOR
32060
CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2C - 2
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV • Figure 2C-2 Sample Loader Flow Diagram
Figure 2C-2
Sample Loader Flow Diagram
12 psi
12" Hg.
5um
Filter
3-Way
Valve
NC
Out
.010" Orifice
Rack Advance
NO
V1
3-Way
Valve
Load Side Cross Transfer
NC
Out
.016" Orifice
NO
V2
3-Way
Valve
NC
Unload Side Cross Transfer
Out
.016" Orifice
NO
V5
3-Way
Valve
Mixer Head Up/Down
To
Air
NC
Out
.016" Orifice
NO
V3
3-Way
Valve
5 psi
NC
Out
Mixer Bladders
NO
V4
Regulator
32061
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2C - 3
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV • Figure 2C-3 Vacuum/Pressure Flow Diagram
Figure 2C-3
Vacuum/Pressure Flow Diagram
REAGENT & WASTE PANEL
TO AIR
MUFFLER
WASTE
62
PRES
PUMP
63
61
64
SENSORS
TO AIR
TO AIR
FILTER
1
DILUENT
RESERVOIR
SHEATH
RESERVOIR
PS1
SENSOR
ACC 1
PRES 1
(12 psi)
12 psi
2 WAY PINCH VALVE NORMALLY OPEN
9 psi
2
2 WAY PINCH VALVE NORMALLY CLOSED
PS2
5 psi
12" Hg
AIR FLOW RESTRICTOR
VAC 2 (Varies)
SENSOR
CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW
ACC 2
PRES 2
(9 psi)
3
TRAP
PS3
SENSOR
ACC 3
PRES 3
(5 psi)
TRAP
TRAP
67
17
TO SAMPLE LOADER
(SL MODEL ONLY)
TO AIR
MUFFLER
VAC
PUMP
VAC 1 (GRN)
PS4
WC-2
WC-4
TO AIR
WC-1
WC-3
SENSOR
ACC 4
18
4
VAC 1
(12" Hg)
DRAIN
5
PS5
31
32
97
98
VAC 1
(GRN)
VAC 2 (BRN)
SENSOR
PRESS 1 (ORN)
ACC 5
VAC 2
(VAR.)
32062
DRAIN
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
VAC 2 (BRN)
Part Number 9140300 − August 1998
2C - 4
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV • Figure 2C-4 Reagent Flow Diagram
Figure 2C-4
Reagent Flow Diagram
REAGENT & WASTE PANEL
BLOOD/OUT, FARSIDE
WBC/OUT
HGB LYSE
DILUENT
WBC LYSE
WBC DIL/IN, FARSIDE
HGB/OUT
BLOOD/IN
EMPTY
SENSORS
HGB DIL/IN
RBC DIL/IN, FARSIDE
RBC/OUT
WBC LYSE
FRONT VALVE
DILUENT
RESERVOIR
REAR VALVE
HGB LYSE
SHEATH
RESERVOIR
28
23
TOP
HGB LYSE (BLU)
FILTER (SMALL)
WBC LYSE (VIO)
66
WBC
FLOW
CELL
91
65
DILUENT (RED)
96
94
DILUENT (RED)
12345
WBC LYSE
95
RBC
MIXING
CHAMBER
MOTOR-X
BLOOD
SAMPLE
VALVE
WBC
20 uL
HGB
12 uL
RBC
1.67 uL
WBC
M. C.
HGB OUT
HGB DIL
22
MOTOR-G
RBC DIL
RBC OUT
HGB
FLOW
CELL
11
500 uL
SAMPLE
INJECTION
SY1
DILUENT/SHEATH
MOTOR-A
SHEATH
HGB LYSE
PRESSURE
SWITCH
WBC LYSE
2 WAY PINCH VALVE NORMALLY OPEN
15
27
OPEN
SAMPLE
PROBE
25
10 mL
RBC
DIL
SYRINGE
SY4
34
AIR FLOW RESTRICTOR
CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
24
33
MOTOR-E
2 WAY PINCH VALVE NORMALLY CLOSED
26
2.5 mL
HGB
LYSE
SY2
2.5 mL
WBC
LYSE
SYRINGE
SY3
MOTOR-D
MOTOR-C
32063
MOTOR-B
CLOSED
MODE
PROBE
Part Number 9140300 − August 1998
2C - 5
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV • Figure 2C-5 Sample Aspiration Flow
Figure 2C-5
Sample Aspiration Flow Diagram
2 WAY PINCH VALVE NORMALLY OPEN
SAMPLE
SENSOR 2
2 WAY PINCH VALVE NORMALLY CLOSED
WBC
20 uL
MOTOR-X
BLOOD
SAMPLE
VALVE
HGB
12 uL
RBC
1.67 uL
AIR FLOW RESTRICTOR
CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW
ULTRASONIC
SENSOR
12
Y-VALVE
47,48
TO AIR
TRAP
17
OPEN
SAMPLE
PROBE
38
WC-2
MOTOR-E
VENT
TRAP
CLOSED
MODE
32064
PS5
VAC 2 (BRN)
SENSOR
OPEN MODE SAMPLE
ACC 5
VAC 2
(VAR.)
CLOSED MODE SAMPLE
OPEN/CLOSED MODE SAMPLE
VAC 2 (BRN)
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
VACUUM #2
Part Number 9140300 − August 1998
2C - 6
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV • Figure 2C-6 Dilution and Mixing Flow
Figure 2C-6
Dilution and Mixing Flow Diagram
(1) RBC/PLT
WBC
FLOW
CELL
TO AIR
FILTER
(LARGE)
1 2345
54
53
52
(2) WBC
WBC
M. C.
51
(3) NOC
55
VENT
ACC
PERISTALTIC
PUMP
MOTOR-G
RBC
MIXING
CHAMBER
HGB
FLOW
CELL
41
32066
2 WAY PINCH VALVE NORMALLY OPEN
RBC/PLT STAGING
WBC STAGING
2 WAY PINCH VALVE NORMALLY CLOSED
AIR FLOW RESTRICTOR
NOC STAGING
STAGING PUMP LINES
WC-1
MIXING CHAMBER VENTS
CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2C - 7
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV • Figure 2C-7 Sample Staging Flow Diagram
Figure 2C-7
Sample Staging Flow Diagram
(1) RBC/PLT
WBC
FLOW
CELL
TO AIR
FILTER
(LARGE)
1 2345
54
53
52
(2) WBC
WBC
M. C.
51
(3) NOC
55
VENT
ACC
PERISTALTIC
PUMP
MOTOR-G
RBC
MIXING
CHAMBER
HGB
FLOW
CELL
41
32066
2 WAY PINCH VALVE NORMALLY OPEN
RBC/PLT STAGING
WBC STAGING
2 WAY PINCH VALVE NORMALLY CLOSED
AIR FLOW RESTRICTOR
NOC STAGING
STAGING PUMP LINES
WC-1
MIXING CHAMBER VENTS
CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2C - 8
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV • Figure 2C-8 Sample Delivery Flow Diagram
Figure 2C-8
Sample Delivery Flow Diagram
SHEATH
RESERVOIR
TOP
TO AIR
FILTER (SMALL)
WBC
FLOW
CELL
91
65
DILUENT (RED)
FILTER
(LARGE)
12345
56
(1) RBC
(2) WBC
(3) NOC
VENT
ACC
500 uL
SAMPLE
INJECTION
SY1
MOTOR-A
68
2 WAY PINCH VALVE NORMALLY OPEN
SAMPLE DELIVERY
FLOW CELL OUT
2 WAY PINCH VALVE NORMALLY CLOSED
WASTE CHAMBER #1 VENT
AIR FLOW RESTRICTOR
SHEATH FLOW
32067
WC-1
CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2C - 9
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV • Figure 2C-9 Waste Flow Diagram
Figure 2C-9
Waste Flow Diagram
BLOOD/OUT, FARSIDE
WBC/OUT
WBC DIL/IN, FARSIDE
HGB/OUT
BLOOD/IN
WASTE
HGB DIL/IN
RBC DIL/IN, FARSIDE
RBC/OUT
FRONT VALVE
REAR VALVE
WBC
FLOW
CELL
12345
56
57
52
WBC
M. C.
WBC
20 uL HGB
12 uL
RBC
1.67 uL
MOTOR-G
RBC
MIXING
CHAMBER
TRAP
HGB
FLOW
CELL
12
92
13
32068
PERISTALTIC
PUMP
TRAP
TRAP
93
Y-VALVE
47,48
21
OPEN
SAMPLE
PROBE
14
WC-2
MOTOR-E
WC-4
WC-1
VENT
TRAP
WC-3
37
ACC 4
VAC 1
(12" Hg)
CLOSED
MODE
PROBE
DRAIN
36
2 WAY PINCH VALVE NORMALLY OPEN
ACC 5
VAC 2
(VAR.)
WASTE (BLK)
WASTE CHAMBER #1 WASTE
WASTE CHAMBER #2 WASTE
2 WAY PINCH VALVE NORMALLY CLOSED
DRAIN
AIR FLOW RESTRICTOR
WASTE CHAMBER #3 WASTE
WASTE CHAMBER #4 WASTE
MAIN WASTE OUT
CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
2C - 10
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV • Figure 2C-10 Vent Flow Diagram
Figure 2C-10 Vent Flow Diagram
TO AIR
2 WAY PINCH VALVE NORMALLY OPEN
MUFFLER
2 WAY PINCH VALVE NORMALLY CLOSED
PRES
PUMP
AIR FLOW RESTRICTOR
TO AIR
TO AIR
FILTER
1
CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW
TO AIR
VENT TO AIR
FILTER
(LARGE)
ACC 1
PRES 1
53
(12 psi)
51
WBC
M. C.
VENT
ACC
RBC
MIXING
CHAMBER
HGB
FLOW
CELL
TO AIR
MUFFLER
68
VAC
TO AIR
PUMP
TO AIR
38
32069
WC-1
ACC 4
VAC 1
(12" Hg)
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
4
VENT
TRAP
Part Number 9140300 − August 1998
2C - 11
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV • Service Commands
Service Commands
DIAGNOSTICS
3200 Hidden Screens
SET POINT ENTRY
From the Menu listed, press the function keys indicated to
access the desired Service Screen.
LATEX/BLOOD COMPARE
TOGGLE CS/SL
Example: To access the Set Point Entry Screen, enter the
DIAGNOSTICS Menu then press F12/F1.
From Main Menu:
Pressing F12/F1 accesses the following
Commands.
DIAG FUNCTIONS
ERROR LOG
F12/F8 to go to C:\CD3200 prompt
STORE RAW ON/OFF
DEBUG ON/OFF
From C:\CD3200
prompt:
Pressing DIAG FUNCTIONS accesses
the following commands.
Type rl to reboot to Initialized
Type rl /nopri to reboot to Ready
PEEK COMMAND
(Use only if Analyzer was in the Ready
Mode before entering DOS.)
POKE COMMAND
MOTOR CONTROL
CALIBRATION
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
F12/F1 accesses Dilution Factors
Part Number 9140300 − August 1998
2C - 12
6#0#3DUWV#/LVWV •#Table of Contents
6#0#3DUWV#/LVWV
Table of Contents
How to Use This Chapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Reagents and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Alphabetical Parts List Cross Reference . . . . . . . . . . . . . . . . . 4
PL-1 Power Supply Module . . . . . . . . . . . . . . . . . . . . . . 11
PL-2 Power Distribution Module & Laser Supply. . . . . . 13
PL-3 Reagent Lines & Fans . . . . . . . . . . . . . . . . . . . . . 16
PL-4 Flow Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
PL-5 Vacuum/Pressure . . . . . . . . . . . . . . . . . . . . . . . . . 22
PL-6 Aspiration Tower & Open Probe Assemblies). . . . 24
PL-7 Syringe Drivers (AB, CD) . . . . . . . . . . . . . . . . . . . 27
PL-8 Peri-Pump, Sample Handler Module #1,
& Open Probe Assembly. . . . . . . . . . . . . . . . . . . . 29
PL-9 Shear Valve Driver, Motor Driver Module,
& Sample Sensor Boards . . . . . . . . . . . . . . . . . . . 31
PL-10 Laser Bench . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
PL-11 Motor Processor Module. . . . . . . . . . . . . . . . . . . . 36
PL-12 Main Amplifier & Signal Processor Modules . . . . . 38
PL-13 Central Processing Unit/Device Control Module. . 40
PL-14 Flow Control Module, View #1 . . . . . . . . . . . . . . . 42
PL-15 Solenoid Driver Modules 1, 2, & Solenoid
Driver Module/Cable Distribution Module . . . . . . . 45
PL-16 Solenoid Driver Modules 5,6,9 & Cable
Distribution Module #2 . . . . . . . . . . . . . . . . . . . . . 48
PL-17 Main Computer Boards & Computer Assemblies . 51
PL-18 Sample Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
PL-19 Covers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Flow Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Pneumatic Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Sample Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Tubing Designator/Catalog Number Table . . . . . . . . . . . . 66
How to Use This Chapter
This chapter contains Parts Identification information for the
CELL-DYN 3200 System. It includes:
•
Alphabetical Listing of Parts and corresponding PL
numbers;
•
Illustrated Parts Lists, showing part location, catalog
number and description.
•
System Tubing Maps with table of tubing designators,
catalog numbers and descriptions.
•
Table of frequently used supplies and tools.
Part Number 9140300 – August 1998
3-1
6#0#3DUWV#/LVWV • Table of Contents
To find information about a part:
•
From the Alphabetical Listing:
–
•
Click on the red PL number to jump to the appropriate
Parts Description/Catalog Number table.
From an illustrated Parts List:
–
•
Find the part in the drawing, note its item number, and
click anywhere on the drawing to jump to the
appropriate Parts Description table.
From a Tubing Map:
–
Find the Tubing Designator on the tubing, e.g., small
silicon tubing is designator S1. Click anywhere on the
Tubing Map to jump to the Tubing Designator/Catalog
Number table.
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3-2
3 - Parts Lists • Reagents and Supplies
Reagents and Supplies
Item
Part Number
Description
1
99120-01
Calibrator, CELL-DYN 3000
2
93111-01
Ctrl, CELL-DYN 3000 (2.5 mL)
3
99129-01
Ctrl, CELL-DYN 3000 (3.0 mL)
4
03H78-01
Reagent, WBC Lyse (960 mL)
5
03H79-01
Reagent, DIL/STH (20 L)
6
03H80-01
Reagent, HGB/NOC (960 mL)
7
99644-01
Enzymatic Cleaner
8
1360-02
ISE Conditioning Solutions
9
8160580701
Versalube
10
8160600301
5.0µ Standard Reference Particles
11
8160600401
7.0µ Standard Reference Particles
12
8160600501
3.0µ Standard Reference Particles
13
06H63-01
Rack, Sample Loader (1 ea)
14
04H87-01
Racks, Sample Loader (5 ea)
15
92532-01
Connector, Ser Loopback
16
06H71-01
Specifications, Interface
17
8938080601
Disks, Installation (1 & 2)
18
8938070001
Disk, Service
19
06H61-01
Disk, Setup
20
06H90-01
Kit, Software Upgrade Dom.
21
06H91-01
Kit, Software Upgrade Int.
22
8945020201
Fixture, Voltage Divider
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3-3
6#0#3DUWV#/LVWV • Alphabetical Parts List Cross Reference
Alphabetical Parts List Cross Reference
Description
List #
Description
List #
Bd, Sensor, Sample Tube
PL-18
Bd, SER Link/KBd Intfc
PL-17
Bd, Shear Valve Driver, SVDM
PL-9
Bd, Signal Processor Module, SPM
PL-12
3.0µ Standard Reference Particles
Rea/Sup
5.0µ Standard Reference Particles
Rea/Sup
7.0µ Standard Reference Particles
Rea/Sup
Bd, Solenoid Driver Module, SDM
PL-15 PL-16
Accumulator, Vac/Pres (Lg)
PL-5
Bd, Status Alert
PL-19
Accumulator, Vac/Pres (SM)
PL-5
Bd, Ultrasonic Sensor
PL-9
Barcode Reader
PL-18
Bd, Video, VGA W/1MB RAM
PL-17
Bd, Cable Dist Module, CDM
PL-16
Bd, VPM (Vac/Pres Module)
PL-5
Bd, Chopper Driver Module, CDB
PL-6
Bd. Sample Handler Module, SHM
PL-8
Bd, CPU/DCM
PL-13
Belt, Timing
PL-7
Bd, DC Motor Driver Module
PL-9
Belt, Timing Spinner Assy
PL-6
Bd, Flow Control Module, FCM
PL-14
Belt, Timing, GS1 Assy
PL-6
Bd, HV/Preamp
PL-10
Belt, Timing, GS1 Motor
PL-6
Bd, I/O Serial/Parallel
PL-17
Bench, Optics (Complete)
PL-10
Bd, In-line Sensor
PL-3
Block, Wash, Closed Sample
PL-6
Bd, Main Amplifier Module
PL-12
Block, Wash, Open Sample
PL-6
Bd, w/RAM & CPU Mother
PL-17
Bottle, Vent Accumulator
PL-4
Bd, Motor Processor Module, MPM
PL-11
Bottle, Waste (large)
PL-4
Bd, PDM (Power Dist. Module)
PL-2
Bottle, Waste (small)
PL-4
Bd, Photodiode Preamp
PL-10
Cable Kit, Internal I/O
PL-17
Bd, PRM (Pump Relay Module)
PL-5
PL-18
Cable, 900 PRE-J2 to MAM-J12
PL-10 PL-12
Bd, Sample Handler Module
Bd, Sample Sensor#2
PL-9
Cable, 900 D PRE-J2 to MAM-J14
PL-10 PL-12
Bd, SDM/CDM
PL-15
Cable, CDM-J6 to FCM-J2
PL-16
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
PL-7
Part Number 9140300 – August 1998
3-4
6#0#3DUWV#/LVWV • Alphabetical Parts List Cross Reference
Description
List #
Description
List #
Cable, CDM2-J10 to SH Sensor
PL-16
Cable, FCM-J26 to ILSB-J1
PL-14 PL-3
Cable, CDM2-J4 to VPM-J6
PL-16
Cable, FCM-J4 to SDM9-J10
PL-14
Cable, CDM2-J7 to Ext Waste
PL-3
Cable, FCM-J5 to PSM(B)-J1
PL-14
Cable, CDM2-J8 to DIL Sensor
PL-16
Cable, FCM-J7 to SAB-J1
PL-14
Cable, CPU-J1 to SHM1-J16
PL-13
Cable, FCM-J8 to SVDM-J2
PL-14
Cable, CPU-J10 to SPM-J5
PL-12
Cable, FCM-J9 to Start SW
PL-14
Cable, CPU-J12 to SPM-J7
PL-13
Cable, Floppy Drive INTFC
PL-17
Cable, CPU-J17 to SHM2-J16
PL-13
Cable, Hard Drive INTFC
PL-17
Cable, CPU-J3 to SPM-J4
PL-13
Cable, HGB Flow Cell to FCM
PL-4
Cable, CPU-J6 to MPM-J1
PL-11 PL-13
Cable, HSSL (Internal)
PL-13
Cable, CPU-J7 to FCM-J25
PL-13
Cable, HSSL to Monitor
PL-17
Cable, CPU-J8 to FCM-J22
PL-13
Cable, Interface, HSSL
PL-17
Cable, DCM2-J3 to PSM(B)-J1
PL-16
Cable, MAM-J10 to PD PRE
PL-12
Cable, External CPU Bus
PL-12
Cable, MAM-J12 to PD PRE
PL-12
Cable, FCM-J-8 to HGB F. Cell
PL-14
Cable, MAM-J15 to SPM-J6
PL-12
Cable, FCM-J1 to CDM1-J6
PL-14
Cable, MAM-J3 to PDM-J4
PL-12
Cable, MUX DAC Ctrl Bus
PL-5
Cable, MAM-J4 to CPU-J9
PL-12
Cable, FCM-J10 to DIL OP Sen
PL-14
Cable, MDM-J1 to SDM4-J8
PL-9
Cable, FCM-J18 to HGB Cell
PL-4
Cable, MDM-J2 to SDM4-J7
PL-9
Cable, FCM-J2 to CDM2-J6
PL-14
Cable, MPM-J3 to CDB A-J1
PL-7 PL-11
Cable, FCM-J21 to PDM-J8
PL-14
Cable, MPM-J4 to CDB B-J1
PL-7 PL-11
Cable, FCM-J22 to CPU-J8
PL-14
Cable, MPM-J5 to CDB C-J1
PL-7
Cable, FCM-J23 to VPM-J13
PL-14
Cable, MPM-J6 to CDB D-J1
PL-7 PL-11
Cable, FCM-J24 to VPM-J14
PL-14
Cable, MPM-J7 to CDB E-J1
PL-11
Cable, FCM-J25 to CPU-J7
PL-14
Cable, MPM-J7 to CDM-J1
PL-6
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
PL-16
PL-13
PL-13
Part Number 9140300 – August 1998
PL-19
PL-13
PL-11
3-5
6#0#3DUWV#/LVWV • Alphabetical Parts List Cross Reference
Description
List #
Cable, MPM-J9 to CDB G-J1
PL-8 PL-11
Cable, MUX DAC CTRL Bus
Description
List #
Cable, SDM Data/+5V Bus
PL-12 PL-13
PL-14
PL-14
PL-16
Cable, SDM5-J10 to CDM2-J1
PL-16
Cable, Nose Cover Sensor
PL-8 PL-19
Cable, SDM5-J10 to CDM2-J2
PL-16
Cable, P/O Ultrasonic Sen
PL-14
Cable, SDM9-J10 to FCM-J4
PL-16
Cable, PDM-J10 to Hard Drive
PL-2 PL-17
Cable, Serial Periph Bus
Cable, PDM-J11 to Floppy Drive
PL-2 PL-17
PL-5 PL-13
PL-14
Cable, PDM-J12 to SHM1-J12
PL-2
Cable, SHM1-J1 to GS2 Solenoid
PL-8
Cable, PDM-J13 to SHM2-J12
PL-2
Cable, SHM1-J12 to PDM-J12
PL-8
Cable, PDM-J2/J3 to Mother Bd.
PL-2 PL-17
Cable, SHM1-J13 to GS1 Motor
PL-8
Cable, PDM-J4 to MAM-J3
PL-2 PL-13
Cable, SHM1-J16 to CPU-J1
PL-8
Cable, PDM-J5 to MDM-J3
PL-2 PL-9
Cable, SHM1-J8 to Spin Motor
PL-8
Cable, PDM-J6 to MPM-J2
PL-2 PL-11
Cable, SHM1-J9 to Sensors
PL-8
Cable, PDM-J8 to FCM-J21
PL-2 PL-5
Cable, SHM2-J10 to MIX Sens
PL-18
Cable, PMT Socket
PL-10
Cable, SHM2-J11 to Tube Sens
PL-18
Cable, Printer, Parallel
PL-17
Cable, SHM2-J12 to PDM-J13
PL-18
Cable, PSM (B)-J1 to CDM2-J3
PL-1
Cable, SHM2-J13 to MIX MTR
PL-18
Cable, PSM (B)-J3 to SVD-J3
PL-1
Cable, SHM2-J15 to BCD RDR
PL-18
Cable, PSM (B)-J4 to PDM-J9
PL-1 PL-2
Cable, SHM2-J16 to CPU-J17
PL-18
Cable, PSM (Back)-J2 to CDM1-J3
PL-1 PL-15
Cable, SHM2-J9 to RACK Sens
PL-18
Cable, PSM (T)-J13 to PWR SW
PL-1
Cable, SPM-J4 to CPU-J3
PL-12
Cable, PSM (T)-J2 to PRM-J4
PL-1
Cable, SS2-J1 to FCM-J14
PL-9 PL-14
Cable, PSM (T)-J501 to PDM-J1
PL-1 PL-2
Cable, SVDM J3 to PSM (B)-J3
PL-9
Cable, PSM (T)-J7 to XXXFAN
PL-1
Cable, SVDM J4 to DC Motor
PL-9
Cable, PSM (T)-J8 to XXXFAN
PL-1
Cable, SVDM-J1 to Sensors
PL-9
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
PL-15
3-6
6#0#3DUWV#/LVWV • Alphabetical Parts List Cross Reference
Description
List #
Description
List #
Cable, SVDM-J2 to FCM-J8
PL-9
Cover, Right Side
PL-19
Cable, Vac1 Wet Sensor
PL-5
Cover, SL Bottom
PL-19
Cable, Vac2 Wet Sensor
PL-5
Cover, SL Left Side Front
PL-19
Cable, VPM-J13 to FCM-J23
PL-5
Cover, SL Right Side Front
PL-19
Cable, VPM-J14 to FCM-J24
PL-5
Cover, SL Top Skirt
PL-19
Cable, VPM-J18 to PRM-J1
PL-5
Cover, Top
PL-19
Cable, VPM-J6 to CDM2-J4
PL-5
Cylinder, Air, Mixer Lift
PL-18
Cable, Waste Full Sensor
PL-3
Cylinder, Air, X & Y Index
PL-18
Cable, Y-Valve Sensor
PL-8 PL-9
Disk Drive, Floppy, 1.44MB
PL-17
Calibrator, CELL-DYN 3000
Rea/Sup
Disk Drive, Hard, 540MB
PL-17
Cap Assy, Diluent/Sheath
PL-3
Disk, Service
Rea/Sup
Cap Assy, HGB/NOC Lyse
PL-3
Disk, Setup
Rea/Sup
Cap Assy, WBC, Lyse
PL-3
Disks, Installation (1 & 2)
Rea/Sup
Cbl, SDM/CDM-J1 to SDM1-J10
PL-15
Driver Assy, Y-Valve, Complete
PL-8
Cbl, SDM/CDM-J2 to SDM2-J10
PL-15
Enzymatic Cleaner
Rea/Sup
Chamber, Mixing, RBC/WBC
PL-4
Fan Guards & Filter, 3 Pcs
PL-3
Clamp, Tubing 1/4 O.D.
TM
Fan, 115VAC, 4.69” SQ
PL-3
Computer Assy, CD3200
PL-17
Filter, 5 Micron, In-line
TM
Connector, Ser Loopback
Rea/Sup
Filter, In-line, Sheath
PL-4
Cord, Power 125Vac 10A
PL-1
Filter, Pressure Pump
PL-5
Cover, Front Nose Section
PL-19
Filter, Vent Accumulator
PL-4
Cover, Keyboard
PL-17
Fitting, Luer Lock
PL-7
Cover, Left Front Door
PL-19
Fitting, Reagent Inlet
PL-3
Cover, Left Side
PL-19
Fitting, Waste Outlet
PL-3
Cover, Right Front Door
PL-19
Fixture, Voltage Divider
Rea/Sup
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3-7
6#0#3DUWV#/LVWV • Alphabetical Parts List Cross Reference
Description
Description
List #
Flow Cell Assy, HGB
PL-4
Flow Cell, Optical
PL-10
Fitting (T4)
TM
Fitting 0.062” X 0.062” X 0.062”
List #
Keyboard, PCAT
PL-17
Kit, Hardware, 3200
PL-4
PL-19
TM
Kit, Reagent Line (Complete)
PL-3
Fitting Barb Conn 0.062" X 0.062"
TM
Kit, Software Upgrade Dom.
Rea/Sup
Fitting Barb RDCR 0.093" X 0.062"
TM
Kit, Software Upgrade Int.
Rea/Sup
Fitting Barb RDCR 0.125" X 0.062"
TM
Knob, Sample Valve
PL-9
Fitting Barb RDCR 0.125" X 0.093"
TM
Laser, HeNe, 5mw
PL-10
Fitting Conn 0.093 X 0.093
TM
Mirror Perforated
PL-10
Fitting Elb 0.062" X 0.062" X 0.062"
TM
Mixer Head, Complete
PL-18
Fitting Elb 0.093 X 0.093
TM
Monitor, 14” VGA Color
PL-17
Fitting, L, 1/8 in x 1/8 in
TM
Motor, Spinner Assy
PL-6
Fitting, Reducer, 3/16 in to 1/8 in
TM
Motor, Stepper
Fitting RSTR .010” X 1/16”
TM
PL-6
PL- 8
Fitting RSTR .016” X 1/16”
TM
Motor, Stepper, GS1
PL-6
Fitting T 0.062" X 0.062" X 0.062"
TM
Motor, Stepper, Mixer
PL-18
Fitting T 0.062" X 0.125" X 0.062"
TM
Motor, Y-Valve
PL-8
Fitting T 0.093 X 0.093 X 0.093
TM
Needle, Closed Sample
PL-6
Fitting T 0.125" X 0.125" X 0.125"
TM
Plug
TM
Fuse, 4A 220/240 (≥200VAC In)
PL-1
Power Supply Laser
PL-2
Fuse, 5A 220/240 Vac
PL-5
Power Supply, AC/DC Main
PL-1
Fuse, 8A 220/240 (<200VAC In)
PL-1
Probe Driver Assy, Open
PL-6
Fuse, Fast 1A 250V 3 AG
PL-2
Probe, Open Sample
PL-6
Fuse, SB 15A 220/240VAC
PL-1
Pump Assy, Peristaltic
PL-8
ISE Cleaning Solutions
Rea/Sup
Pump Head, Vac/Pres
PL-5
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
PL-5
PL-7
3-8
6#0#3DUWV#/LVWV • Alphabetical Parts List Cross Reference
Description
List #
Description
List #
Pump Roller Assy
PL-8
Switch, Reed, w/Cable
PL-18
Pump, Pressure (Complete)
PL-5
Switch, Touch Bar
PL-19
Pump, Vacuum (Complete)
PL-5
Syringe Driver Assy, (AB)
PL-7
Rack, Sample Loader (1 ea)
Rea/Sup
Syringe, Diluent (10 mL)
PL-7
Racks, Sample Loader (5 ea)
Rea/Sup
Syringe, Driver Assy, (CD)
PL-7
Reagent, DIL/STH (20 L)
Rea/Sup
Syringe, Sample Inject, 500 uL
PL-7
Reagent, HGB/NOC (960 mL)
Rea/Sup
Syringe, WBC/HGB (2.5 mL)
PL-7
Reagent, WBC Lyse (960 mL)
Rea/Sup
Tubing Silicon (S2)
TM
Regulator, Pressure
PL-18
Tubing Silicon (SY)
TM
Reservoir, Diluent/Sheath
PL-4
Tubing Silicon 1/32 ID x 5/32 OD
TM
Sample Loader, Complete
PL-18
Tubing Tyg 0.062" ID 0.125" OD Pur
TM
Sensor, Overpressure
PL-4
Tubing Tyg 0.062" ID 0.125" OD Red
TM
Sensor, Photodiode & LED
PL-6 PL-8
PL-18 PL-19
Tubing Tyg 0.062”
TM
Tubing Tyg 0.125 ID 0.250 OD Grn
TM
Sensor, Sample Detector
PL-9
Tubing Tyg 0.125 “ID 0.250” OD Yel
TM
Sensor, Sample, Ultrasonic
PL-8
Tubing Tyg 0.125" ID 0.250" 0D Pur
TM
Shear Valve Driver Assy
PL-9
Tubing Tyg 0.125" ID 0.250" OD Blk
TM
Shield, SHM #1, 2
PL-18
Tubing Tyg 0.125" ID 0.250" OD Grn
TM
Smpl Valve Ceramic, Center
PL-9
Tubing Tyg 0.125" ID 0.250" OD Or
TM
Smpl Valve Ceramic, Front
PL-9
Tubing Tyg 0.125" ID 0.250" OD Red
TM
Smpl Valve Ceramic, Rear
PL-9
Tubing Tyg 0.125” ID 0.250 OD Blu
TM
Solenoid, GS2 Stop
PL-6
Tubing Tyg 1/32” ID 3/32 OD
TM
Speaker Assy
PL-17
Tubing Tyg 3/16” ID 5/16” OD Clr
TM
Specifications, Interface
Rea/Sup
Tubing Tyg 3/32”
TM
Switch, AC Input, 16A
PL-17
Tubing Tyg O.125” ID 0.250” OD Gry
TM
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3-9
6#0#3DUWV#/LVWV •#Alphabetical Parts List Cross Reference
Description
List #
Tubing Tygon, 1/16” ID
TM
Tubing Tygon, 1/8” ID
TM
Tower Assy, Aspiration
PL-6
Trap Assy, Waste Bottle
PL-4
Tube Assy, Waste Outlet
PL-3
Tube, Photomult, 900
PL-10
Tube, Photomult, 900 DEP
PL-10
Tubing, Peristaltic Pump
PL-8
Valve Assy, Solenoid, 3.9KG
PL-4
Valve Assy, Solenoid, 3.9KG
PL-5
Valve Assy, Solenoid, 5Kg
PL-5
Valve Assy, Solenoid, NC
PL-4
Valve, Check, Duckbill
TM
Valve, Rotary, 3-Port
PL-8
Valve, Solenoid, 3-Way
PL-18
Versalube™
Rea/Sup
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 10
6#0#3DUWV#/LVWV •#PL-1 Power Supply Module (1 of 2)
PL-1 Power Supply Module (1 of 2)
8
7
9
10
11
6
13/14
5
4
3
2
CELL-DYN 3200 SYSTEM SERVICE MANUAL
1
12
Part Number 9140300 – August 1998
3 - 11
6#0#3DUWV#/LVWV •#PL-1 Power Supply Module (2 of 2)
PL-1 Power Supply Module (2 of 2)
Item
Part Number
Description
1
2
3
4
5
6
7
8
9
10
11
12
13
8934000801
8951078201
8952086901
8952082801
8952086601
8510016601
8952087801
8952062001
8952000101
8952000201
8952089601
8240051601
50065-01
Power Supply, AC/DC Main
Cable, PSM (B)-J4 to PDM-J9
Cable, PSM (B)-J3 to SVD-J3
Cable, PSM (Back)-J2 to CDM1-J3
Cable, PSM (B)-J1 to CDM2-J3
Fuse, SB 15A 220/240VAC
Cable, PSM (T)-J2 to PRM-J4
Cable, PSM (T)-J8 to XXXFAN
Cable, PSM (T)-J7 to XXXFAN
Cable, PSM (T)-J13 to PWR SW
Cable, PSM (T)-J501 to PDM-J1
Cord, Power 125Vac 10A
Fuse, 8A 220/240 (<200VAC In)
14
61164-01
Fuse, 4A 220/240 (≥200VAC In)
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 12
6#0#3DUWV#/LVWV •#PL-2 Power Distribution Module & Laser Supply (1 of 3)
PL-2 Power Distribution Module & Laser Supply (1 of 3)
5
6
4
7
8
9
10
11
3
2
1
CELL-DYN 3200 SYSTEM SERVICE MANUAL
12
Part Number 9140300 – August 1998
3 - 13
6#0#3DUWV#/LVWV •#PL-2 Power Distribution Module & Laser Supply (2 of 3)
PL-2 Power Distribution Module & Laser Supply (2 of 3)
13
14
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 14
6#0#3DUWV#/LVWV •#PL-2 Power Distribution Module & Laser Supply (3 of 3)
PL-2 Power Distribution Module & Laser Supply (3 of 3)
Item
1
2
3
4
5
6
7
8
9
10
11
12
13
14
Part Number
8952078201
8952082901
8952054301
8952089601
8952089701
8952089801
8952061201
8952086501
8952078901
8952089401
8952089501
8960198001
8510019201
8921207601
Description
Cable, PSM (B)-J4 to PDM-J9
Cable, PDM-J8 to FCM-J21
Cable, PDM-J6 to MPM-J2
Cable, PSM (T)-J501 to PDM-J1
Cable, PDM-J10 to Hard Drive
Cable, PDM-J11 to Floppy Drive
Cable, PDM-J3 to Mother Bd.
Cable, PDM-J4 to MAM-J3
Cable, PDM-J5 to MDM-J3
Cable, PDM-J12 to SHM1-J12
Cable, PDM-J13 to SHM2-J12
Bd, PDM (Power Dist. Module)
Fuse, Fast 1A 250V 3 AG
Power Supply Laser
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 15
6#0#3DUWV#/LVWV •#PL-3 Reagent Lines & Fans (1 of 3)
PL-3 Reagent Lines & Fans (1 of 3)
1
4
3
6
CELL-DYN 3200 SYSTEM SERVICE MANUAL
2
5
Part Number 9140300 – August 1998
3 - 16
6#0#3DUWV#/LVWV •#PL-3 Reagent Lines & Fans (2 of 3)
PL-3 Reagent Lines & Fans (2 of 3)
10
9
9
7
11
8
13
32017
CELL-DYN 3200 SYSTEM SERVICE MANUAL
12
Part Number 9140300 – August 1998
3 - 17
6#0#3DUWV#/LVWV •#PL-3 Reagent Lines & Fans (3 of 3)
PL-3 Reagent Lines & Fans (3 of 3)
Item
1
2
3
4
5
6
7
8
9
10
11
12
13
Part Number
06H65-01
8921210101
8921205901
8921211501
92161-02
8925089901
8960091001
8952087001
03484-01
8260402701
8952085701
8930038301
8930067601
Description
Kit, Reagent Line (Complete)
Cap Assy, WBC, Lyse
Cap Assy, Diluent/Sheath
Cap Assy, HGB/NOC Lyse
Tube Assy, Waste Outlet
Cable, Waste Full Sensor
Bd, In-line Sensor
Cable, FCM-J26 to ILSB-J1
Fan Guards & Filter, 3 Pcs
Fan, 115VAC, 4.69” SQ
Cable, CDM2-J7 to Ext Waste
Fitting, Reagent Inlet
Fitting, Waste Outlet
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 18
6#0#3DUWV#/LVWV •#PL-4 Flow Panel (1 of 3)
PL-4 Flow Panel (1 of 3)
3
1
1
2
3
2
2
2
2
2
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 19
6#0#3DUWV#/LVWV •#PL-4 Flow Panel (2 of 3)
PL-4 Flow Panel (2 of 3)
7
9
8
14
6
5
4
13
15
11
9
CELL-DYN 3200 SYSTEM SERVICE MANUAL
4
10
12
32019
Part Number 9140300 – August 1998
3 - 20
6#0#3DUWV#/LVWV •#PL-4 Flow Panel (3 of 3)
PL-4 Flow Panel (3 of 3)
Item
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Part Number
8921122501
8921016301
8913007601
8921174702
8270721901
8921176502
8921174902
8921206702
8921150901
8510615001
8921174802
8270721901
8921210801
8952077801
8921217201
Description
Valve Assy, Solenoid, 3.9KG
Valve Assy, Solenoid, NC
Kit, Hardware, 3200
Bottle, Waste (small)
Filter, Vent Accumulator
Bottle, Vent Accumulator
Reservoir, Diluent/Sheath
Flow Cell Assy, HGB
Trap Assy, Waste Bottle
Sensor, Overpressure
Bottle, Waste (large)
Filter, In-line, Sheath
Chamber, Mixing, RBC/WBC
Cable, HGB Flow Cell to FCM
Bottle, Waste (small w/tubing)
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 21
6#0#3DUWV#/LVWV •#PL-5 Vacuum/Pressure (1 of 2)
PL-5 Vacuum/Pressure (1 of 2)
9
10
11
13
8
12
3
22
V1
3
3
7
V4
V2
V3
V5
2
4
6
4
15
16
1
1
5
14
17
2
19
1
18
21
20
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 22
6#0#3DUWV#/LVWV •#PL 5 Vacuum/Pressure (2 of 2)
PL 5 Vacuum/Pressure (2 of 2)
Item
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
Part #
8921122001
8913007601
8921173201
8921122101
8921153401
8921153001
8410301801
60160-01
8960120001
8952083401
8921173401
8952087501
8952052801
8952082901
8952083101
8952083201
8952084101
8952084901
8952087901
8952083401
8960122001
8270040301
Description
Valve Assy, Solenoid, 5Kg
Kit, Hardware, 3200
Accumulator, Vac/Pres (SM)
Valve Assy, Solenoid, 3.9KG
Pump, Vacuum (Complete)
Pump, Pressure (Complete)
Pump Head, Vac
Fuse, 5A 220/240 Vac
Bd, PRM (Pump Relay Module)
Cable, VPM-J18 to PRM-J1
Accumulator, Vac/Pres (Lg)
Cable, Vac2 Wet Sensor
Cable, Vac1 Wet Sensor
Cable, PDM-J8 to FCM-J21/VPM-J12
Cable, VPM-J13 to FCM-J23
Cable, VPM-J14 to FCM-J24
Cable, MUX DAC CTRL Bus
Cable, Serial Periph Bus
Cable, VPM-J6 to CDM2-J4
Cable, VPM-J18 to PRM-J1
Bd, VPM (Vac/Pres Module)
Filter, Pressure Pump
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 23
6#0#3DUWV#/LVWV •#PL-6 Aspiration Tower & Open Probe Assemblies (1 of 3)
PL-6 Aspiration Tower & Open Probe Assemblies (1 of 3)
1
7
2
10
3
5
8
4
9
2
CELL-DYN 3200 SYSTEM SERVICE MANUAL
6
Part Number 9140300 – August 1998
3 - 24
6#0#3DUWV#/LVWV •#PL-6 Aspiration Tower & Open Probe Assemblies (2 of 3)
PL-6 Aspiration Tower & Open Probe Assemblies (2 of 3)
16
11
12
15
13
14
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 25
6#0#3DUWV#/LVWV •#PL-6 Aspiration Tower & Open Probe Assemblies (3 of 3)
PL-6 Aspiration Tower & Open Probe Assemblies (3 of 3)
Item
Part #
Description
1
8921208502
Tower Assy, Aspiration
2
8370830101
Sensor, Photodiode & LED
3
8250426601
Belt, Timing, GS1 Assy
4
8250426501
Belt, Timing, GS1 Motor
5
8921209202
Motor, Spinner Assy
6
8250426801
Belt, Timing Spinner Assy
7
03H99-01
Needle, Closed Sample
8
8933040501
Block, Wash, Closed Smple
9
8934005801
Solenoid, GS2 Stop
10
8350602701
Motor, Stepper, GS1
11
8921204601
Probe Driver Assy, Open
12
9960042001
Bd, Chopper Driver Module, CDB
13
8952084201
Cable, MPM-J7 to CDM-J1
14
8921076101
Motor, Stepper
15
8931201401
Block, Wash, Open Sample
16
8930094601
Probe, Open Sample
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 26
6#0#3DUWV#/LVWV •#PL-7 Syringe Drivers (AB, CD) (1 of 2)
PL-7 Syringe Drivers (AB, CD) (1 of 2)
8
5
5
4
4
7
6
9
9
4
2
4
10
9
6
12
13
9
3
1
2
CELL-DYN 3200 SYSTEM SERVICE MANUAL
6
5
11
Part Number 9140300 – August 1998
6
5
3 - 27
6#0#3DUWV#/LVWV •#PL-7 Syringe Drivers (AB, CD) (2 of 2)
PL-7 Syringe Drivers (AB, CD) (2 of 2)
Item
Part #
Description
1
8921217301
Syringe Driver Assy, (AB)
2
28561-01
Syringe, WBC/HGB (2.5 mL)
3
28560-01
Syringe, Sample Inj (500 uL)
4
04H02-01
Fitting, Luer Lock
5
8250425701
Belt, Timing
6
8921076101
Motor, Stepper
7
8952084401
Cable, MPM-J4 to CDB-J1
8
8952015401
Cable, MPM-J6 to CDB D-J1
9
8960042001
Bd, Chopper Driver Module, CDB
10
8952015501
Cable, MPM-J5 to CDB C-J1
11
8921217401
Syringe, Driver Assy, (CD)
12
02H82-01
Syringe, Diluent (10 mL)
13
8952084501
Cable, MPM-J3 to CDB A-J1
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 28
6#0#3DUWV#/LVWV •#PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (1 of 2)
PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (1 of 2)
11
12
13
10
9
14
8
7
6
15
1
2
16
16
3
5
CELL-DYN 3200 SYSTEM SERVICE MANUAL
4
17
Part Number 9140300 – August 1998
19
18
17
3 - 29
6#0#3DUWV#/LVWV •#PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (2 of 2)
PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (2 of 2)
Item
Part #
Description
1
8921137801
Pump Assy, Peristaltic
2
8952056301
Cable, MPM-J9 to CDB G-J1
3
8921076101
Motor, Stepper
4
91485-01
Tubing, Peristaltic Pump
5
8921071001
Pump Roller Assy
6
8960191001
Bd. Sample Handler Module, SHM
7
8952089201
Cable, SHM1-J16 to CPU-J1
8
8952088301
Cable, SHM1-J13 to GS1 MOT
9
8952089401
Cable, SHM1-J12 to PDM-J12
10
8952088401
Cable, SHM1-J1 to GS2 SOL
11
8952088201
Cable, SHM1-J8 to Spin Motor
12
8952078001
Cable, Nose Cover Sensor
13
8952087201
Cable, SHM1-J9 to Sensors
14
8921206101
Driver Assy, Y-Valve, Complete
15
8921207701
Motor, Y-Valve
16
8370830101
Sensor, Photodiode & LED
17
8952087101
Cable, Y-Valve Sensor
18
8250900301
Valve, Rotary, 3-Port
19
8370181801
Sensor, Sample, Ultrasonic
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 30
6#0#3DUWV#/LVWV •#PL-9 Shear Valve Driver, Motor Driver Mod, & Sample Sensor Bds (1 of 3)
PL-9 Shear Valve Driver, Motor Driver Module, & Sample Sensor Boards (1 of 3)
8
9
10
11
12
1
4
7
5
6
CELL-DYN 3200 SYSTEM SERVICE MANUAL
3
Part Number 9140300 – August 1998
2
3 - 31
6#0#3DUWV#/LVWV •#PL-9 Shear Valve Driver, Motor Driver Mod, & Sample Sensor Bds (2 of 3)
PL-9 Shear Valve Driver, Motor Driver Module, & Sample Sensor Boards (2 of 3)
18
19
P/O Bd.
20
13
17
16
15
CELL-DYN 3200 SYSTEM SERVICE MANUAL
14
Part Number 9140300 – August 1998
3 - 32
6#0#3DUWV#/LVWV •#PL-9 Shear Valve Driver, Motor Driver Mod, & Sample Sensor Bds (3 of 3)
PL-9 Shear Valve Driver, Motor Driver Module, & Sample Sensor Boards (3 of 3)
Item
Part #
Description
1
8921204901
Shear Valve Driver Assy
2
8960116701
Sensor, Sample Detector
3
N/A
Smpl Valve Ceramics, 3 PCS
4
8932102801
Smpl Valve Ceramic, Rear
5
06H64-01
Smpl Valve Ceramic, Center
6
8932103101
Smpl Valve Ceramic, Front
7
8921167901
Knob, Sample Valve
8
8960222001
Bd, Shear Valve Driver, SVDM
9
8952086201
Cable, SVDM-J2 to FCM-J8
10
8952078501
Cable, SVDM-J1 to Sensors
11
8952086901
Cable, SVDM J3 to PSM (B)-J3
12
8952087401
Cable, SVDM J4 to DC Motor
13
8960194001
Bd, DC Motor Driver Module
14
8952078901
Cable, PDM-J5 to MDM-J3
15
8952079601
Cable, MDM-J2 to SDM4-J7
16
8952079501
Cable, MDM-J1 to SDM4-J8
17
8952087101
Cable, Y-Valve Sensor
18
8370181801
Bd, Ultrasonic Sensor
19
8960116701
Bd, Sample Sensor#2
20
8952082001
Cable, SS2-J1 to FCM-J14
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 33
6#0#3DUWV#/LVWV •#PL-10 Laser Bench (1 of 2)
PL-10 Laser Bench (1 of 2)
3
2
4
5
1
10
6
5
8
7
7
9
11
7
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 34
6#0#3DUWV#/LVWV •#PL-10 Laser Bench (2 of 2)
PL-10 Laser Bench (2 of 2)
Item
Part #
Description
1
8921206401
Bench, Optics (Complete)
2
8010131201
Tube, Photomult, 900
3
8921137501
Laser, HeNe, 5mw
4
8570000101
Tube, Photomult, 900 DEP
5
8960100001
Bd, Photodiode Preamp
6
8960101001
Bd, HV/Preamp
7
8921142901
Cable, PMT Socket
8
8952085501
Cable, 900 PRE-J2 to MAM-J12
9
8952085601
Cable, 900D PRE-J2 to MAM-J14
10
8921145201
Flow Cell, Optical
11
8921181501
Mirror Perforated
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 35
6#0#3DUWV#/LVWV •#PL-11 Motor Processor Module (1 of 2)
PL-11 Motor Processor Module (1 of 2)
2
1
3
4
5
6
7
8
9
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 36
6#0#3DUWV#/LVWV •#PL-11 Motor Processor Module (2 of 2))
PL-11 Motor Processor Module (2 of 2))
Item
Part #
Description
1
8960092001
Bd, Motor Processor, MPM
2
8952084701
Cable, CPU-J6 to MPM-J1
3
8952054301
Cable, PDM-J6 to MPM-J2
4
8952084501
Cable, MPM-J3 to CDB A-J1
5
8952084401
Cable, MPM-J4 to CDB B-J1
6
8952015501
Cable, MPM-J5 to CDB C-J1
7
8952015401
Cable, MPM-J6 to CDB D-J1
8
8952084201
Cable, MPM-J7 to CDB E-J1
9
8952056301
Cable, MPM-J9 to CDB G-J1
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 37
6#0#3DUWV#/LVWV •#PL-12 Main Amplifier & Signal Processor Modules (1 of 2)
PL-12 Main Amplifier & Signal Processor Modules (1 of 2)
1
2
3
13
8
12
11
10
6
9
8
7
6
CELL-DYN 3200 SYSTEM SERVICE MANUAL
5
Part Number 9140300 – August 1998
4
3 - 38
6#0#3DUWV#/LVWV •#PL-12 Main Amplifier & Signal Processor Modules (2 of 2)
PL-12 Main Amplifier & Signal Processor Modules (2 of 2)
Item
Part #
Description
1
8960195001
Bd, Main Amplifier, MAM
2
8952085501
Cable, 900 PRE-J2 to MAM-J12
3
8952085601
Cable, 900 D PRE-J2 to MAM-J14
4
8960183001
Bd, Signal Processor, SPM
5
8952086501
Cable, MAM-J3 to PDM-J4
6
8952084801
Cable, MAM-J15 to SPM-J6
7
8952050001
Cable, SPM-J4 to CPU-J3
8
8952084101
Cable, MUX DAC CTRL Bus
9
8952050501
Cable, CPU-J10 to SPM-J5
10
8952084601
Cable, External CPU Bus
11
8952085401
Cable, MAM-J12 to PD PRE
12
8952085301
Cable, MAM-J10 to PD PRE
13
8952050601
Cable, MAM-J4 to CPU-J9
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 39
6#0#3DUWV#/LVWV •#PL-13 Central Processing Unit/Device Control Module (1 of 2)
PL-13 Central Processing Unit/Device Control Module (1 of 2)
1
2
6
4
3
5
9
7
11
12
13
15
10
14
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 40
6#0#3DUWV#/LVWV •#PL-13 Central Processing Unit/Device Control Module (2 of 2)
PL-13 Central Processing Unit/Device Control Module (2 of 2)
Item
Part #
Description
1
8960182001
Bd, CPU/DCM
2
8952084901
Cable, Serial Periph Bus
3
8952086401
Cable, HSSL (Internal)
4
8952084701
Cable, CPU-J6 to MPM-J1
5
8952084601
Cable, External CPU Bus
6
8952089301
Cable, CPU-J17 to SHM2-J16
7
8952089201
Cable, CPU-J1 to SHM1-J16
8
8952084101
Cable, MUX DAC CTRL Bus
9
8952050001
Cable, CPU-J3 to SPM-J4
10
8952086501
Cable, PDM-J4 to MAM-J3
11
8952085001
Cable, CPU-J12 to SPM-J7
12
8952050601
Cable, MAM-J4 to CPU-J9
13
8952085201
Cable, CPU-J7 to FCM-J25
14
8952050501
Cable, CPU-J10 to SPM-J5
15
8952085101
Cable, CPU-J8 to FCM-J22
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 41
6#0#3DUWV#/LVWV •#PL-14 Flow Control Module, View #1 (1 of 3)
PL-14 Flow Control Module, View #1 (1 of 3)
1
10
9
8
7
5
6
4
2
3
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 42
6#0#3DUWV#/LVWV •#PL-14 Flow Control Module, View #1 (2 of 3)
PL-14 Flow Control Module, View #1 (2 of 3)
21
20
19
18
16
17
15
14
13
CELL-DYN 3200 SYSTEM SERVICE MANUAL
12
11
Part Number 9140300 – August 1998
3 - 43
6#0#3DUWV#/LVWV •#PL-14 Flow Control Module, View #1 (3 of 3)
PL-14 Flow Control Module, View #1 (3 of 3)
Item
Part #
Description
1
8960132001
Bd, Flow Control Module, FCM
2
8952084101
Cable, MUX DAC CTRL Bus
3
8952084901
Cable, Serial Periph Bus
4
8952082501
Cable, FCM-J1 to CDM1-J6
5
8952082601
Cable, FCM-J2 to CDM2-J6
6
8952087001
Cable, FCM-J26 to ILSB-J1
7
8952083701
Cable, SDM Data/+5V Bus
8
8952086101
Cable, FCM-J4 to SDM9-J10
9
8952086601
Cable, FCM-J5 to PSM(B)-J1
10
8952083801
Cable, FCM-J7 to SAB-J1
11
8952082901
Cable, FCM-J21 to PDM-J8
12
8952085101
Cable, FCM-J22 to CPU-J8
13
8952083101
Cable, FCM-J23 to VPM-J13
14
8952083201
Cable, FCM-J24 to VPM-J14
15
8952085201
Cable, FCM-J25 to CPU-J7
16
8952038501
Cable, FCM-J-8 to HGB F. Cell
17
8952082001
Cable, SS2-J1 to FCM-J14
18
N/A
Cable, P/O Ultrasonic Sen
19
8952083901
Cable, FCM-J10 to DIL OP Sen
20
8952078301
Cable, FCM-J9 to Start SW
21
8952086201
Cable, FCM-J8 to SVDM-J2
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 44
6#0#3DUWV#/LVWV •#PL-15 Solenoid Dr Mod 1, 2, & Solenoid Dr Mod/Cable Distrib Module (1 of 3)
PL-15 Solenoid Driver Modules 1, 2, & Solenoid Driver Module/Cable
Distribution Module (1 of 3)
MDM-J1
MDM-J2
2
1
6
V41
V38
V32
V36
5
V34
V33
4
V32 V31
3
6
6
3
CELL-DYN 3200 SYSTEM SERVICE MANUAL
5
6
Part Number 9140300 – August 1998
4
3 - 45
6#0#3DUWV#/LVWV •#PL-15 Solenoid Dr Mod 1, 2, & Solenoid Dr Mod/Cable Distrib Module (2 of 3)
PL-15 Solenoid Driver Modules 1, 2, & Solenoid Driver Module/Cable
Distribution Module (2 of 3)
V18
V21
V17
V22
V23
V15
V24
V25
V14
V13
V11 V12
CELL-DYN 3200 SYSTEM SERVICE MANUAL
V26
Part Number 9140300 – August 1998
V27 V28
3 - 46
6#0#3DUWV#/LVWV •#PL-15 Solenoid Dr Mod 1, 2, & Solenoid Dr Mod/Cable Distrib Module (3 of 3)
PL-15 Solenoid Driver Modules 1, 2, & Solenoid Driver Module/Cable
Distribution Module (3 of 3)
Item
Part #
Description
1
8960205001
Bd, SDM/CDM
2
8952082801
Cable, PSM (Back)-J2 to CDM1-J3
3
8952083001
Cbl, SDM/CDM-J1 to SDM1-J10
4
8952085801
Cbl, SDM/CDM-J2 to SDM2-J10
5
8960093001
Bd, Solenoid Driver Module, SDM
6
8952083701
Cable, SDM Data/+5V Bus
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 47
6#0#3DUWV#/LVWV •#PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (1 of 3)
PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (1 of 3)
11
12
1
1
2
1
11
8
10
9
2
8
CELL-DYN 3200 SYSTEM SERVICE MANUAL
7
6
5
Part Number 9140300 – August 1998
3
4
3 - 48
6#0#3DUWV#/LVWV •#PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (2 of 3)
PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (2 of 3)
V98
V97
V96
V95
V94
V93
V92 V91
V68
V67
V57
V56
V66
V55
V65
V54
V64
V63
V62
V61
CELL-DYN 3200 SYSTEM SERVICE MANUAL
V53
V52
Part Number 9140300 – August 1998
V51
3 - 49
6#0#3DUWV#/LVWV •#PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (3 of 3)
PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (3 of 3)
Item
Part #
Description
1
8960093001
Bd, Solenoid Driver Module, SDM
2
8952085901
Cable, SDM5-J10 to CDM2-J1
3
8960108001
Bd, Cable Dist Module, CDM
4
8952085701
Cable, CDM2-J7 to EXT Waste
5
8952083501
Cable, CDM2-J8 to DIL Sensor
6
8952083601
Cable, CDM2-J10 to SH Sensor
7
8952082601
Cable, CDM-J6 to FCM-J2
8
8952086001
Cable, SDM5-J10 to CDM2-J2
9
8952086601
Cable, DCM2-J3 to PSM(B)-J1
10
8952087901
Cable, CDM2-J4 to VPM-J6
11
8952083701
Cable, SDM Data/+5V Bus
12
8952086101
Cable, SDM9-J10 to FCM-J4
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 50
6#0#3DUWV#/LVWV •#PL-17 Main Computer Boards & Computer Assemblies (1 of 4)
PL-17 Main Computer Boards & Computer Assemblies (1 of 4)
1
4
3
5
2
7
CELL-DYN 3200 SYSTEM SERVICE MANUAL
6
Part Number 9140300 – August 1998
3 - 51
6#0#3DUWV#/LVWV •#PL-17 Main Computer Boards & Computer Assemblies (2 of 4)
PL-17 Main Computer Boards & Computer Assemblies (2 of 4)
15
15
14
11
10
16
8
3
9
13
12
CELL-DYN 3200 SYSTEM SERVICE MANUAL
6
Part Number 9140300 – August 1998
3 - 52
6#0#3DUWV#/LVWV •#PL-17 Main Computer Boards & Computer Assemblies (3 of 4)
PL-17 Main Computer Boards & Computer Assemblies (3 of 4)
19
20
18
17
20
22
16
21
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
21
3 - 53
6#0#3DUWV#/LVWV •#PL-17 Main Computer Boards & Computer Assemblies (4 of 4)
PL-17 Main Computer Boards & Computer Assemblies (4 of 4)
Item
Part #
Description
1
8200570201
Disk Drive, Floppy, 1.44MB
2
8921150601
Speaker Assy
3
8952056201
Cable, Floppy Drive INTFC
4
8952089801
Cable, PDM-J11 to Floppy Drive
5
8200570701
Disk Drive, Hard, 540MB
6
8952064801
Cable, Hard Drive INTFC
7
8952089701
Cable, PDM-J10 to Hard Drive
8
8921205401
Computer Assy, CD3200
9
8921211901
Bd Mother, w/RAM & CPU
10
8952061201
Cable, PDM-J2/J3 to Mother Bd
11
8170001301
Bd, Video, VGA w/1MB RAM
12
8960109001
Bd, SER Link/KBd INTFC
13
8952082401
Cable, HSSL to Monitor
14
8170000701
Bd, I/O Serial/Parallel
15
8600028501
Cable Kit, Internal I/O
16
06H74-01
Keyboard, PCAT
17
06H75-01
Cover, Keyboard
18
04H44-01
Monitor, 14” VGA Color
19
8510751801
Switch, AC Input, 16A
20
8952087017
Cable, Interface, HSSL
21
20005-01
Cable, Printer, Parallel
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 54
6#0#3DUWV#/LVWV •#PL-18 Sample Loader (1 of 5)
PL-18 Sample Loader (1 of 5)
10
32070
9
#
7
13
#
11
12
16
15
14
2
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 55
6#0#3DUWV#/LVWV •#PL-18 Sample Loader (2 of 5)
PL-18 Sample Loader (2 of 5)
19
18
#
7
11
17
19
32071
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 56
6#0#3DUWV#/LVWV •#PL-18 Sample Loader (3 of 5)
PL-18 Sample Loader (3 of 5)
7
19
7
11
32072
11
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 57
6#0#3DUWV#/LVWV •#PL-18 Sample Loader (4 of 5)
PL-18 Sample Loader (4 of 5)
2
3
1
4
5
8
7
6
V5
V4
32073
V3
V2
V1
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 58
6#0#3DUWV#/LVWV • PL-18 Sample Loader (5 of 5)
PL-18 Sample Loader (5 of 5)
Item
Part #
Description
1
8960191001
Bd, Sample Handler Module
2
8952058801
Cable, SHM2-J11 to Tube Sens
3
8952058601
Cable, SHM2-J15 to BCD RDR
4
8952089301
Cable, SHM2-J16 to CPU-J17
5
8952089001
Cable, SHM2-J13 to MIX MTR
6
8952089501
Cable, SHM2-J12 to PDM-J13
7
8952088701
Cable, SHM2-J9 to RACK Sens
8
Part of Sensor
Cable, SHM2-J10 to MIX Sens
9
8921211601
Sample Loader, Complete
10
8350602501
Motor, Stepper, Mixer
11
8370830101
Sensor, Photodiode & LED
12
8934001701
Barcode Reader
13
8921208301
Mixer Head, Complete
14
8960211001
Bd, Sensor, Sample Tube
15
2880860801
Cylinder, Air, Mixer Lift
16
8510510101
Switch, Reed, w/Cable
17
8450876601
Valve, Solenoid, 3-Way
18
8310842201
Regulator, Pressure
19
8280861301
Cylinder, Air, X & Y Index
20
8932074001
Shield, SHM #1, 2
CELL-DYN® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 59
6#0#3DUWV#/LVWV •#PL-19 Covers (1 of 3)
PL-19 Covers (1 of 3)
6
7
8
9
4
5
3
6
2
17
8
13
12
8
1
CELL-DYN 3200 SYSTEM SERVICE MANUAL
8
32074
Part Number 9140300 – August 1998
11
10
3 - 60
6#0#3DUWV#/LVWV •#PL-19 Covers (2 of 3)
PL-19 Covers (2 of 3)
11
20
19
18
32075
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 61
6#0#3DUWV#/LVWV •#PL-19 Covers (3 of 3)
PL-19 Covers (3 of 3)
Item
Part #
Description
1
8931213001
Cover, SL Bottom
2
8932063701
Cover, SL Top Skirt
3
8932073701
Cover, SL Right Side Front
4
8932073801
Cover, SL Left Side Front
5
8921217001
Cover, Front Nose Section
6
8913007601
Kit, Hardware, 3200
7
8931204801
Cover, Left Side
8
8913007601
Kit, Hardware, 3200
9
8931204601
Cover, Top
10
8931204701
Cover, Right Side
11
8932062201
Cover, Right Front Door
12
8370830101
Sensor, Photodiode & LED
13
8952078001
Cable, Nose Cover Sensor
17
8932062101
Cover, Left Front Door
18
8510810901
Switch, Touch Bar
19
8952083801
Cable, FCM-J7 to SAB-J1
20
8960192001
Bd, Status Alert
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 62
6#0#3DUWV#/LVWV •#Flow Panel
Flow Panel
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 63
6#0#3DUWV#/LVWV •#Pneumatic Unit
Pneumatic Unit
L
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 64
6#0#3DUWV#/LVWV •#Sample Loader
Sample Loader
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 65
6#0#3DUWV#/LVWV •#Tubing Designator/Catalog Number Table
Tubing Designator/Catalog Number Table
Item
Part #
Description
A1
8310817501
Fitting RSTR .016” X 1/16”
A2
8310817401
Fitting RSTR .010” X 1/16”
C
8310808401
Fitting Conn 0.093 X 0.093
C1
8310802001
Fitting Barb Conn 0.062" X 0.062"
CLP2
8210050201
Clamp, Tubing 1/4 O.D.
CV
8310810201
Valve, Check, Duckbill
EL
8310820001
Fitting Elb 0.093 X 0.093
F1
8270722201
Filter, 5 Micron, In-line
L1
8310807901
Fitting Elb 0.062" X 0.062" X 0.062"
L7
8310805801
Fitting, L, 1/8 in x 1/8 in
P1
03H85-01
Plug
R1
8310805901
Fitting Barb RDCR 0.093" X 0.062"
R2
8310800101
Fitting Barb RDCR 0.125" X 0.062"
R3
8310806201
Fitting Barb RDCR 0.125" X 0.093"
R4
8310807401
Fitting, Reducer, 3/16 in to 1/8 in
S1
9130542
Tubing, Silicon 1/32 ID x 5/32 OD
S2
9130543
Tubing Silicon (S2)
SY
9130544
Tubing Silicon (SY)
T1
8310801001
Fitting T 0.062" X 0.062" X 0.062"
T3
8310800901
Fitting T 0.125" X 0.125" X 0.125"
T4
31082-01
Fitting (T4)
T5
8310809501
Fitting T 0.062" X 0.125" X 0.062"
T8
8310800701
Fitting T 0.093 X 0.093 X 0.093
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 66
6#0#3DUWV#/LVWV •#Tubing Designator/Catalog Number Table
Item
Part #
Description
Y1
9130548
Tubing Tygon, 1/16” ID
Y2
9130549
Tubing Tygon, 1/8” ID
Y3
8310654201
Tubing Tygon 3/32”
Y4
9130555
Tubing Tygon 1/32” ID 3/32 OD
Y5
8310654001
Tubing Tygon 3/16” ID 5/16” OD Clr
Y622
8310662201
Tubing Tygon 0.062" ID 0.125" OD Red
Y626
8310662601
Tubing Tygon 0.062”
Y627
8310662701
Tubing Tygon 0.062" ID 0.125" OD Pur
Y630
8310663001
Tubing Tygon 0.125" ID 0.250" OD Blk
Y631
8310663101
Tubing Tygon 0.125 ID 0.250 OD Grn
Y632
8310663201
Tubing Tygon 0.125" ID 0.250" OD Red
Y633
8310663301
Tubing Tygon 0.125" ID 0.250" OD Or
Y634
8310663401
Tubing Tygon 0.125 “ID 0.250” OD Yel
Y635
8310663501
Tubing Tygon 0.125" ID 0.250" OD Grn
Y636
8310663601
Tubing Tygon 0.125” ID 0.250 OD Blu
Y637
8310663701
Tubing Tygon 0.125" ID 0.250" 0D Pur
Y638
8310663801
Tubing Tygon O.125” ID 0.250” OD Gry
Y7
8310653501
Tubing Tygon 3/32”
Y FIT
8310810801
Fitting 0.062” X 0.062” X 0.062”
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 – August 1998
3 - 67
7#0#5HPRYDO#)#5HSODFHPHQW
Table of Contents
Introduction
Shear Valve & Shear Valve Driver (RR-1). . . . . . . . . . . . . . . . .2
Y-Valve (RR-2) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Ultrasonic Sensor (RR-3) . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
Open Probe Drive Assembly (RR-4) . . . . . . . . . . . . . . . . . . . .22
Optics Bench (RR-5) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
Power Supply (RR-6). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
Vacuum/Pressure Assembly (RR-7) . . . . . . . . . . . . . . . . . . . .39
Tower Assembly (RR-8) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
HGB Flow Cell (RR-9) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52
Sample Loader (RR-10) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
Syringe Driver Assembly (RR-11) . . . . . . . . . . . . . . . . . . . . . .69
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Step-by-step subassembly removal procedures for field
replaceable components are described on the following illustrated
pages. Most subassemblies are replaced in reverse order of
disassembly, and reassembly is obvious and straightforward.
Part Number 9140300 -- August 1998
4-1
7#5HPRYDO#)#5HSODFHPHQW#• Shear Valve & Shear Valve Driver (RR-1)
Shear Valve & Shear Valve
Driver (RR-1)
Figure 4-1:
Front Access Doors
Removal Procedure
1. Ensure that the shear valve is in the
cleaning position using the special
protocols function.
2. With analyzer power off, open the left
and right access doors and remove
the safety cover.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4-2
7#5HPRYDO#)#5HSODFHPHQW#• Shear Valve & Shear Valve Driver (RR-1)
3. Remove the shear valve retaining
screw from the center of the shear
valve.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-2:
Shear Valve Retaining Screw
Part Number 9140300 − August 1998
4-3
7#5HPRYDO#)#5HSODFHPHQW#• Shear Valve & Shear Valve Driver (RR-1)
4. Carefully remove the three ceramic
pieces from the shear valve. Set the
fragile center piece aside. Do not
disconnect tubing from the other two
pieces.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-3:
Shear Valve Ceramics.
Part Number 9140300 − August 1998
4-4
7#5HPRYDO#)#5HSODFHPHQW#• Shear Valve & Shear Valve Driver (RR-1)
5. Remove the top cover (4 screws) and
the optics cover (two 6-32 screws and
two 8-32 screws.)
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-4:
Removing Top Cover and Optics Cover
Part Number 9140300 − August 1998
4-5
7#5HPRYDO#)#5HSODFHPHQW#• Shear Valve & Shear Valve Driver (RR-1)
6. Remove the 3 screws that hold the
assembly in place.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-5:
Shear Valve Driver MountingScrews
Part Number 9140300 − August 1998
4-6
7#5HPRYDO#)#5HSODFHPHQW#• Shear Valve & Shear Valve Driver (RR-1)
7. Move the preamplifier cables out of
the way and disconnect cables at J2
and J3 from the Driver Bd.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-6:
Shear Valve Cables J2 and J3.
Part Number 9140300 − August 1998
4-7
7#5HPRYDO#)#5HSODFHPHQW#• Shear Valve & Shear Valve Driver (RR-1)
8. Angle the assembly and remove from
the back
Figure 4-7:
Shear Valve & Shear Valve Driver Removed.
Replacement Procedure
Replace by following the steps above in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4-8
7#5HPRYDO#)#5HSODFHPHQW#• Y-Valve (RR-2)
Y-Valve (RR-2)
Figure 4-8:
Front Access Doors
Removal Procedure
1. With analyzer power off, open the left
and right access doors and remove
the front cover.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4-9
7#5HPRYDO#)#5HSODFHPHQW#• Y-Valve (RR-2)
2. Locate the Y-valve assembly and cut
the tie wrap on the lower left-hand
corner.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-9:
Y-Valve Tie Wrap
Part Number 9140300 − August 1998
4 - 10
7#5HPRYDO#)#5HSODFHPHQW#• Y-Valve (RR-2)
3. Disconnect the tubing from the three
ports on the valve block
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-10: Y-Valve Block Tubing
Part Number 9140300 − August 1998
4 - 11
7#5HPRYDO#)#5HSODFHPHQW#• Y-Valve (RR-2)
4. Remove the two screws at the top
near the motor and cut the tie wrap to
the left of the wash block.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-11: Y-Valve Mounting Screws
Part Number 9140300 − August 1998
4 - 12
7#5HPRYDO#)#5HSODFHPHQW#• Y-Valve (RR-2)
5. Disconnect the two sensor
connections from the base of the
assembly.
Figure 4-12: Y-Valve Sensor Connections.
NOTE: For replacement purposes,
note which socket each comes from
as they are not identified.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 13
7#5HPRYDO#)#5HSODFHPHQW#• Y-Valve (RR-2)
6. Remove the top cover (4 screws).
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-13: Top Cover Removal
Part Number 9140300 − August 1998
4 - 14
7#5HPRYDO#)#5HSODFHPHQW#• Y-Valve (RR-2)
7. Cut the two tie wraps from around the
cables above solenoids and the tie
wrap beneath solenoid 13. Disconnect
cable J6 from the Motor Driver Bd.
(located to the left of solenoid 11 in
photo.)
Figure 4-14: Y-Valve Cable J6
Feed cable through front panel and
remove assembly.
Replacement Procedure
Follow the above steps in reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 15
7#5HPRYDO#)#5HSODFHPHQW#• Ultrasonic Sensor (RR-3)
Ultrasonic Sensor (RR-3)
Figure 4-15: Front Access Doors
Removal Procedure
1. With analyzer power off, open the left
and right access doors and remove
the front cover.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 16
7#5HPRYDO#)#5HSODFHPHQW#• Ultrasonic Sensor (RR-3)
2. Remove the top cover (4 screws.)
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-16: Top Cover Removal
Part Number 9140300 − August 1998
4 - 17
7#5HPRYDO#)#5HSODFHPHQW#• Ultrasonic Sensor (RR-3)
3. Locate the ultrasonic sensor and cut
the tie wrap on the Y-valve
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-17: Y-Valve Tie Wrap
Part Number 9140300 − August 1998
4 - 18
7#5HPRYDO#)#5HSODFHPHQW#• Ultrasonic Sensor (RR-3)
4. Remove the two fluid connections
from the left and right of the ultrasonic
sensor.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-18: Ultrasonic Sensor Fluid Connections
Part Number 9140300 − August 1998
4 - 19
7#5HPRYDO#)#5HSODFHPHQW#• Ultrasonic Sensor (RR-3)
5. Remove the two screws on the front
panel which hold the Sensor Bd.
bracket.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-19: Ultrasonic Sensor Holding Screws
Part Number 9140300 − August 1998
4 - 20
7#5HPRYDO#)#5HSODFHPHQW#• Ultrasonic Sensor (RR-3)
6. Disconnect the gray wires from J1 on
the Sensor Bd and feed connector
through front panel.
Figure 4-20: Ultrasonic Sensor Board Connectors
Replacement Procedure
Follow the above steps in reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 21
7#5HPRYDO#)#5HSODFHPHQW#• Open Probe Drive Assembly (RR-4)
Open Probe Drive Assembly
Figure 4-21: Front Access Doors
(RR-4)
Removal Procedure
Warning Potential Biohazard:
The aspiration needle/probe is sharp
and potentially contaminated with
infectious materials. Avoid contact
with the tips of the needle/probe.
1. With analyzer power off, open the left
and right access doors and remove
the front cover.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 22
7#5HPRYDO#)#5HSODFHPHQW#• Open Probe Drive Assembly (RR-4)
2. Locate and remove the two screws
holding the wash block to the open
probe assembly and let the wash
block dangle.
Figure 4-22: Wash Block Holding Screws
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 23
7#5HPRYDO#)#5HSODFHPHQW#• Open Probe Drive Assembly (RR-4)
3. Remove the 4 screws holding the
assembly to the front panel.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-23: Open Probe Assembly Mounting Screws
Part Number 9140300 − August 1998
4 - 24
7#5HPRYDO#)#5HSODFHPHQW#• Open Probe Drive Assembly (RR-4)
4. Remove the top cover (4 screws).
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-24: Top Cover Removal
Part Number 9140300 − August 1998
4 - 25
7#5HPRYDO#)#5HSODFHPHQW#• Open Probe Drive Assembly (RR-4)
5. Remove the right side cover
(4 screws).
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-25: Right Side Cover Removal
Part Number 9140300 − August 1998
4 - 26
7#5HPRYDO#)#5HSODFHPHQW#• Open Probe Drive Assembly (RR-4)
6. Loosen the upper and lower
thumbscrews and open the CPU door
assembly to gain access to the back of
the aspiration assembly.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-26: CPU Door Access
Part Number 9140300 − August 1998
4 - 27
7#5HPRYDO#)#5HSODFHPHQW#• Open Probe Drive Assembly (RR-4)
7. Carefully pull aside the white solenoid
wires to remove the drive assembly,
twisting it to the right to remove.
Disconnect the ribbon cable J1 from
the Chopper Driver Bd. and remove
the Probe Assembly.
Figure 4-27: Probe Assembly Removal
Replacement Procedure
Replace by following the above steps in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 28
7#5HPRYDO#)#5HSODFHPHQW#• Optics Bench (RR-5)
Optics Bench (RR-5)
Figure 4-28: Top Cover & Optics Cover Removal
Removal Procedure
'$1*(5=
High Voltage: Assure the
power to the instrument is
off. A high voltage charge
may remain on the laser
connector with the power
off. Do not touch the male
pins. Short male pins to
Chassis.
1. With the analyzer power off, remove
the top cover (4 screws) and the optics
cover (4 screws).
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 29
7#5HPRYDO#)#5HSODFHPHQW#• Optics Bench (RR-5)
2. Remove the five optics mounting
screws. Remove screws from the
aluminum plate, not the black base of
the Optics Bench.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-29: Optics Bench Mounting Screws
Part Number 9140300 − August 1998
4 - 30
7#5HPRYDO#)#5HSODFHPHQW#• Optics Bench (RR-5)
3. Remove the 4 signal cables from the
optics bench. Lay the bundle away
from the optics.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-30: Optics Signal Cables
Part Number 9140300 − August 1998
4 - 31
7#5HPRYDO#)#5HSODFHPHQW#• Optics Bench (RR-5)
4. Remove the two mixing chambers. Do
not disconnect the tubing. Loosen the
two flow cell panel thumbscrews and
open the panel.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-31: Flow Cell Panel
Part Number 9140300 − August 1998
4 - 32
7#5HPRYDO#)#5HSODFHPHQW#• Optics Bench (RR-5)
5. Disconnect the flow cell tubing (1 - 5)
from the manifold.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-32: Flow Cell Tubing
Part Number 9140300 − August 1998
4 - 33
7#5HPRYDO#)#5HSODFHPHQW#• Optics Bench (RR-5)
6. Being careful not to stress any wires
that may be connected to the main
power supply, lift the optics bench out
and place on top of back left corner of
the chassis. Disconnect the brown and
yellow wire from top of the main power
supply and remove optics bench.
Figure 4-33: Optics Bench Removal
Replacement Procedure
Replace by following the above steps in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 34
7#5HPRYDO#)#5HSODFHPHQW#• Power Supply (RR-6)
Power Supply (RR-6)
Figure 4-34: Power Supply Connections
Removal Procedure
1. With analyzer power off, remove
optics bench (RR-5).
2. Disconnect the nine connectors
shown on the power supply.
Note 1: Five-pin connections J1, J2,
and J3 are the same.
Note 2: Three-pin connection is
directly above an empty two-pin
connection.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 35
7#5HPRYDO#)#5HSODFHPHQW#• Power Supply (RR-6)
3. Remove power supply mounting
screws (6 places).
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-35: Power Supply Mounting Screws
Part Number 9140300 − August 1998
4 - 36
7#5HPRYDO#)#5HSODFHPHQW#• Power Supply (RR-6)
4. Lift power supply up and out.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-36: Power Supply Removal
Part Number 9140300 − August 1998
4 - 37
7#5HPRYDO#)#5HSODFHPHQW#• Power Supply (RR-6)
Replacement Procedure
Figure 4-37: Power Supply Line Frequency & Line Voltage Select Switches
1. Ensure Line Frequency and Line
Voltage Select switches are set
correctly, according to the photo.
2. Ensure fuse is present and OK.
3. To reinstall, follow the removal steps in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 38
7#5HPRYDO#)#5HSODFHPHQW#• Vacuum/Pressure Assembly (RR-7)
Vacuum/Pressure Assembly
(RR-7)
Figure 4-38: Left Side Panel Removal
Removal Procedure
NOTE: It is no longer necessary to
remove the Vacuum/Pressure
Assembly when servicing the VPM,
pumps, etc. The Assembly is mounted
on movable slides in 3200 analyzers
built after May, 1998. To access the
Assembly:
1. With the analyzer power off, remove
the left side panel (4 screws).
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 39
7#5HPRYDO#)#5HSODFHPHQW#• Vacuum/Pressure Assembly (RR-7)
2. Disconnect the fan power cord and
remove fan assembly (4 screws).
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-39: Fan Assembly Removal
Part Number 9140300 − August 1998
4 - 40
7#5HPRYDO#)#5HSODFHPHQW#• Vacuum/Pressure Assembly (RR-7)
3. Remove the locking screw and pull
vacuum/pressure assembly out until
unit locks in place.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-40: Vacuum/Pressure Assembly Removal
Part Number 9140300 − August 1998
4 - 41
7#5HPRYDO#)#5HSODFHPHQW#• Vacuum/Pressure Assembly (RR-7)
Replacement Procedure
Figure 4-41: Vacuum/Pressure Assembly Return
1. To return assembly to normal position,
push up on clip and push assembly
back in place.
Verification Procedure
See Verification Matrix, Section 5.2.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 42
7#5HPRYDO#)#5HSODFHPHQW#• Tower Assembly (RR-8)
Tower Assembly (RR-8)
Figure 4-42: Front Access Doors
Removal Procedure
Warning Potential Biohazard:
The aspiration needle/probe is sharp
and potentially contaminated with
infectious materials. Avoid contact
with the tips of the needle/probe.
1. With the analyzer power off, open the
left and right access doors and
remove the safety cover.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 43
7#5HPRYDO#)#5HSODFHPHQW#• Tower Assembly (RR-8)
1. Remove the left and right panels from
the rear rail of the sample loader.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-43: Sample Loader Panels
Part Number 9140300 − August 1998
4 - 44
7#5HPRYDO#)#5HSODFHPHQW#• Tower Assembly (RR-8)
2. Remove the screw at the top of the
tower assembly.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-44: Tower Assembly Screw
Part Number 9140300 − August 1998
4 - 45
7#5HPRYDO#)#5HSODFHPHQW#• Tower Assembly (RR-8)
3. Loosen the two screws at each end of
the sample loader just one turn.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-45: Sample Loader End Screws
Part Number 9140300 − August 1998
4 - 46
7#5HPRYDO#)#5HSODFHPHQW#• Tower Assembly (RR-8)
4. Lift sample loader and slide forward
until it stops (about 2”).
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-46: Slide Sample Loader Forward
Part Number 9140300 − August 1998
4 - 47
7#5HPRYDO#)#5HSODFHPHQW#• Tower Assembly (RR-8)
5. Cut the two tie wraps around cable
bundle in front of flow cell panel.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-47: Tower Cable Tie Wraps
Part Number 9140300 − August 1998
4 - 48
7#5HPRYDO#)#5HSODFHPHQW#• Tower Assembly (RR-8)
6. Disconnect J1, J8, J10 and J13 from
the SHM Bd mounted to the flow cell
panel.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-48: Tower Assembly Cables
Part Number 9140300 − August 1998
4 - 49
7#5HPRYDO#)#5HSODFHPHQW#• Tower Assembly (RR-8)
7. Perform the following steps:
a. Disconnect the tubing at aspiration
probe and wash block
Figure 4-49: Tower Assembly Tubing
b. Remove the glass bubble trap (2
screws). Do not disconnect tubing.
c. Lift the tubing from under the clip.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 50
7#5HPRYDO#)#5HSODFHPHQW#• Tower Assembly (RR-8)
8. Remove the three hex head (7/64”
allen) screws from the base of the
Tower Assembly and lift the assembly
out.
Figure 4-50: Tower Assembly Removal
NOTE: Long screw at base.
Replacement Procedure
Replace by following the above steps in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 51
7#5HPRYDO#)#5HSODFHPHQW#• HGB Flow Cell (RR-9)
HGB Flow Cell (RR-9)
Figure 4-51: Front Access Doors
Removal Procedure
1. With analyzer power off, open left and
right access doors and remove front
cover.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 52
7#5HPRYDO#)#5HSODFHPHQW#• HGB Flow Cell (RR-9)
2. Remove tubing from the flow cell: two
in front, one on top and one at the
base.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-52: HGB Flow Cell Tubing
Part Number 9140300 − August 1998
4 - 53
7#5HPRYDO#)#5HSODFHPHQW#• HGB Flow Cell (RR-9)
3. Disconnect the inline electrical
connection.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-53: HGB Flow Cell Electrical Connection
Part Number 9140300 − August 1998
4 - 54
7#5HPRYDO#)#5HSODFHPHQW#• HGB Flow Cell (RR-9)
4. Remove the two screws on top of the
HGB flow cell to remove the flow cell
cover.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-54: HGB Flow Cell Cover Screws
Part Number 9140300 − August 1998
4 - 55
7#5HPRYDO#)#5HSODFHPHQW#• HGB Flow Cell (RR-9)
5. Remove the two screws at back of the
flow cell to remove it.
Figure 4-55: HGB Flow Cell Removal
Replacement Procedure
Replace by following the above steps in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 56
7#5HPRYDO#)#5HSODFHPHQW#• Sample Loader (RR-10)
Sample Loader (RR-10)
Figure 4-56: Front Access Doors
Removal Procedure
Cell-Dyn 3200 Analyzers built after May
1998 do not require sample loader removal
to access front panel.
Follow Part A to access front panel. Follow
Parts A and B to remove and replace the
loader.
Part A
1. With analyzer power off, open the left
and right access doors and remove
the safety cover.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 57
7#5HPRYDO#)#5HSODFHPHQW#• Sample Loader (RR-10)
2. Remove the left and right panels from
the rear rail of the sample loader.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-57: Sample Loader Panels
Part Number 9140300 − August 1998
4 - 58
7#5HPRYDO#)#5HSODFHPHQW#• Sample Loader (RR-10)
3. Remove the screw at the top of the
tower assembly.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-58: Tower Assembly Screw
Part Number 9140300 − August 1998
4 - 59
7#5HPRYDO#)#5HSODFHPHQW#• Sample Loader (RR-10)
4. Loosen the two screws at each end of
the sample loader just one turn.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-59: Sample Loader End Screws
Part Number 9140300 − August 1998
4 - 60
7#5HPRYDO#)#5HSODFHPHQW#• Sample Loader (RR-10)
5. Lift sample loader and slide forward
until it stops (about 2”).
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-60: Slide Sample Loader Forward
Part Number 9140300 − August 1998
4 - 61
7#5HPRYDO#)#5HSODFHPHQW#• Sample Loader (RR-10)
Part B
Figure 4-61: Tower Assembly Base Screws
1. Remove the three hex head screws
(7/64” allen) from the base of the
tower assembly.
NOTE: Larger screw at base.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 62
7#5HPRYDO#)#5HSODFHPHQW#• Sample Loader (RR-10)
2. Release the clip around the tower
assembly tubing. Disconnect the two
tubes as shown in the inset. Lay the
assembly on top of the instrument.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-62: Tower Assembly Tubing
Part Number 9140300 − August 1998
4 - 63
7#5HPRYDO#)#5HSODFHPHQW#• Sample Loader (RR-10)
3. Disconnect the vacuum and pressure
connections by pressing on the metal
clip.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-63: Vacuum & Pressure Connections
Part Number 9140300 − August 1998
4 - 64
7#5HPRYDO#)#5HSODFHPHQW#• Sample Loader (RR-10)
4. Remove plastic cover from SHM.
Disconnect all cables from SHM.
Remove cable clamp (1 screw).
Figure 4-64: SHM Cables
NOTE: Cables are marked for proper
reassembly.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 65
7#5HPRYDO#)#5HSODFHPHQW#• Sample Loader (RR-10)
5. Remove cable clamps. Set aside
ribbon cable (J16) and multicolored
cable (J12).
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-65: Remove Cable Clamps
Part Number 9140300 − August 1998
4 - 66
7#5HPRYDO#)#5HSODFHPHQW#• Sample Loader (RR-10)
6. Remove loader surround cover by
loosening front three screws and
removing left and right end screws (2
at each end).
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-66: Sample Loader Cover Removal
Part Number 9140300 − August 1998
4 - 67
7#5HPRYDO#)#5HSODFHPHQW#• Sample Loader (RR-10)
7. Remove loader mounting screws (two
at each end). Lift loader from brackets.
Figure 4-67: Sample Loader Mounting Screws
Replacement Procedure
Replace by following the above steps in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 68
7#5HPRYDO#)#5HSODFHPHQW#• Syringe Driver Assembly (RR-11)
Syringe Driver Assembly
(RR-11)
Figure 4-68: Front Access Doors
Removal Procedure
1. With analyzer power off, open the left
and right access doors and remove
the safety cover.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 69
7#5HPRYDO#)#5HSODFHPHQW#• Syringe Driver Assembly (RR-11)
2. Remove the right panel from the rear
rail of the sample loader.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-69: Sample Loader Right Panel
Part Number 9140300 − August 1998
4 - 70
7#5HPRYDO#)#5HSODFHPHQW#• Syringe Driver Assembly (RR-11)
3. Remove the screw at the top of the
tower assembly.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-70: Tower Assembly Screw
Part Number 9140300 − August 1998
4 - 71
7#5HPRYDO#)#5HSODFHPHQW#• Syringe Driver Assembly (RR-11)
4. Loosen the two screws at each end of
the sample loader just one turn.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-71: Sample Loader End Screws
Part Number 9140300 − August 1998
4 - 72
7#5HPRYDO#)#5HSODFHPHQW#• Syringe Driver Assembly (RR-11)
5. Lift sample loader and slide forward
until it stops (about 2”).
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-72: Slide Sample Loader Forward
Part Number 9140300 − August 1998
4 - 73
7#5HPRYDO#)#5HSODFHPHQW#• Syringe Driver Assembly (RR-11)
6. Unscrew the luer fittings from top of
each syringe.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-73: Luer Fittings
Part Number 9140300 − August 1998
4 - 74
7#5HPRYDO#)#5HSODFHPHQW#• Syringe Driver Assembly (RR-11)
7. Remove the four mounting screws for
whichever of the two syringe driver
assemblies needs replacing. (Right
side mounting screws shown here.)
Then remove the spacer bar (two
screws) between the two syringe
driver assemblies.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Figure 4-74: Syringe Driver & Spacer Bar
Part Number 9140300 − August 1998
4 - 75
7#5HPRYDO#)#5HSODFHPHQW#• Syringe Driver Assembly (RR-11)
8. Pull out the drive assembly and
disconnect the ribbon cables on each
side.
Figure 4-75: Chopper Driver Ribbon Cables
Replacement Procedure
Replace by following the above steps in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
CELL-DYN ® 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
4 - 76
8#0#9HULILFDWLRQ#3URFHGXUHV • Table of Contents
8#0#9HULILFDWLRQ#3URFHGXUHV
Table of Contents
5.1
5.2
5.3
How to Use This Chapter . . . . . . . . . . . . . . . . . . . . . 2
Verification Matrix . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Verification Procedures . . . . . . . . . . . . . . . . . . . . . . 5
VP-1 System Voltage Verification/Adjustment. . . . . . . . 5
VP-2 A/D Verification/10 Volt Reference
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
VP-3 MAM Reference Voltage Verification/
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
VP-4 VPM Reference Voltage Verification/
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
VP-5 HGB Current Verification/Adjustment . . . . . . . . . . 8
VP-6 Motor Power Verification . . . . . . . . . . . . . . . . . . . 8
VP-7 Motor Operation Verification. . . . . . . . . . . . . . . . . 9
VP-8 Motor Homing Verification . . . . . . . . . . . . . . . . . . 9
VP-9 Solenoid Bank Verification . . . . . . . . . . . . . . . . . 10
VP-10 Individual Solenoid Verification. . . . . . . . . . . . . . 10
VP-11 Bar Code Reader Verification/Alignment . . . . . . 10
VP-12 Specimen Sensor #2 Verification/Adjustment . . 11
VP-13 LSI Loopback Verification. . . . . . . . . . . . . . . . . . 11
VP-14 MAM & SPM Signal Generator Verification . . . . 12
VP-15 Vacuum & Pressure Retention Verification . . . . 12
VP-16 Vacuum & Pressure Level
Verification/Adjustment. . . . . . . . . . . . . . . . . . . . 13
VP-17 Vacuum & Pressure Recovery Verification. . . . . 13
VP-18 Laser Replacement/Alignment Procedure . . . . . 14
VP-19 Front/Rear Mirror Replacement/Alignment
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
VP-20 Optical Flow Cell Verification/Fine Alignment. . . 17
VP-21 Optical Flow Cell Coarse Alignment . . . . . . . . . . 21
CELL-DYN 3200 SYSTEM SERVICE MANUAL
VP-22
VP-23
VP-24
VP-25
VP-26
VP-27
VP-28
VP-29
VP-30
VP-31
VP-32
VP-33
VP-34
VP-35
VP-36
VP-37
VP-38
VP-39
VP-40
VP-41
VP-42
VP-43
VP-44
VP-45
VP-46
VP-47
VP-48
VP-49
PMT Dynode Voltage Verification/Adjustment . . 22
WBC Gain Verification/Adjustment . . . . . . . . . . . 23
WBC OPTI-CAL Verification/Adjustment. . . . . . . 24
NOC Gain Adjustment. . . . . . . . . . . . . . . . . . . . . 26
RBC/PLT 0° Gain Verification/Adjustment . . . . . 27
RBC/PLT 10° Gain Verification/Adjustment . . . . 28
MAM 0° & 10° Log Amplifier Verification . . . . . . . 29
0° MAM Log Amplifier Verification/Adjustmen. . . 32
10° MAM Log Amplifier Verification/
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Aspirate/Vent Needle Verification . . . . . . . . . . . . 34
GS2 Stop Solenoid Verification . . . . . . . . . . . . . . 35
Bar Code Spin Assembly Verification . . . . . . . . . 35
Tube Height Sensors (S1/S2) Verification. . . . . . 35
Mixer Up/Down Verification . . . . . . . . . . . . . . . . . 36
Mixer Head Rotation Verification . . . . . . . . . . . . . 36
Mixer Bladders Verification . . . . . . . . . . . . . . . . . 37
Rack Advance & Tube Sensors Verification . . . . 37
Cross Transfer Arms & Rack Sensors
Verification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Rack Bar Code Labels Verification . . . . . . . . . . . 38
Mixer Bladders Pressure Verification/
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Shear Valve Driver Lubrication Procedure . . . . . 39
Optics Bench Cleaning Procedure . . . . . . . . . . . 40
Optical Flow Cell Cleaning & De-Bubble
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Optical Flow Cell Wetting Procedure. . . . . . . . . . 45
Laser Power Output Verification . . . . . . . . . . . . . 46
Optical Offsets Verification . . . . . . . . . . . . . . . . . 47
Shear Valve Cleaning Procedure . . . . . . . . . . . . 47
HGB Flow Cell Cleaning Procedure . . . . . . . . . . 48
Part Number 9140300 − August 1998
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8#0#9HULILFDWLRQ#3URFHGXUHV • 5.1 How to Use This Chapter
VP-50 Open Mode Aspiration Path Cleaning
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
VP-51 Closed Mode Aspiration Path Cleaning
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
VP-52 Power-Up CPU/DCM 7-Segment LED
Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
VP-53 Software Installation Procedure . . . . . . . . . . . . .
VP-54 Hard Drive Verification . . . . . . . . . . . . . . . . . . . .
VP-55 Floppy Drive Verification. . . . . . . . . . . . . . . . . . .
VP-56 Background Counts Verification . . . . . . . . . . . . .
VP-57 System CV Verification. . . . . . . . . . . . . . . . . . . .
VP-58 System Calibration Verification/Adjustment . . . .
VP-59 Vacuum Accumulator #1 Rinsing Procedure . . .
VP-60 Vacuum Accumulator #2 Rinsing Procedure . . .
VP-61 Hard Drive Formatting . . . . . . . . . . . . . . . . . . . .
VP-62 DOS Installation . . . . . . . . . . . . . . . . . . . . . . . . .
VP-63 CS/SL SETUP . . . . . . . . . . . . . . . . . . . . . . . . . .
VP-64 Backup Procedure . . . . . . . . . . . . . . . . . . . . . . .
VP-65 Restore Setup. . . . . . . . . . . . . . . . . . . . . . . . . . .
VP-66 List Mode Data Collection. . . . . . . . . . . . . . . . . .
VP-67 Download HSSL Com. . . . . . . . . . . . . . . . . . . . .
User and System Conventions
49
50
Menu selections are identified by square bullets. For example:
51
51
51
52
53
53
54
54
55
56
57
57
58
58
58
59
5.1 How to Use This Chapter
This chapter contains:
•
Verification Matrix containing all VPs required to ensure
System integrity after a part is replaced
•
Step-by-step illustrated procedures to verify, align, calibrate,
and maintain components, assemblies, and functions of the
CD3200 Analyzer and Autoloader
–
–
Menu Selections
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ TOWER TEST
or:
a. Press
„
ACCEPT
to enter the new factors for the parameters that need
calibration.
User Entries
User-typed entries are shown in bold, italicized type. For
example:
a. At C:\ prompt type CD\RAWFILE, and press
„
ENTER
b. Type DIR, and Press
„
ENTER
A list of conditions indicating the need to perform a
particular procedure
Required tools and materials for each procedure
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
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8#0#9HULILFDWLRQ#3URFHGXUHV • 5.1 How to Use This Chapter
5.2 Verification Matrix
REPLACED ASSEMBLY
ASSOCIATED VPs
(Perform In Order Listed)
VP-35, VP-36, VP-37, VP-38,
VP-39, VP-40, VP-41
REPLACED ASSEMBLY
ASSOCIATED VPs
(Perform In Order Listed)
Sample Handler Module #2
0 Degree Preamp
VP-23, VP-19, VP-26, VP-56,
VP-57, VP-58
HGB Flow Cell
10 Degree Preamp
VP-23, VP-19, VP-27, VP-56,
VP-57, VP-58
Motor Driver Module
VP-7
Sample Sensor #2
VP-12
90 Degree PMT Preamp
VP-22, VP-23, VP-56, VP-57,
VP-58
Pump Relay Module
VP-17, VP-16
VP-22, VP-23, VP-56, VP-57,
VP-58
Barcode Reader
VP-10, VP-40
90 Degree PMT Preamp
Aspiration Tower
VP-31, VP-32, VP-33, VP-34
Main Amplifier Module
VP-3, VP-28, VP-23, VP-26,
VP-27, VP-56, VP-57, VP-58
Sample Loader
VP-35, VP-36, VP-37, VP-38,
VP-39, VP-40, VP-41
Signal Processor Module
VP-23,VP-26, VP-27, VP-56,
VP-57, VP-58
Optics Bench
CPU/DCM
VP-67, VP-52, VP-2, VP-56,
VP-57, VP-58
VP-45, VP-22, VP-20, VP-23,
VP-24, VP-25, VP-26, VP-27,
VP-56, VP-57, VP-58
Laser Head
VP-18,VP-20, VP-23, VP-24,
VP-25, VP-26, VP-27, VP-56,
VP-57, VP-58
Optics Bench Mirror
VP-19, VP-20, VP-23, VP-24,
VP-25, VP-26, VP-27, VP-56,
VP-57, VP-58
VP-19, VP-20, VP-23, VP-24,
VP-25, VP-26, VP-27
Flow Control Module
Vacuum/Pressure Module
Motor Processor Module
Power Supply Module
VP-5, VP-9
VP-4, VP-16, VP-56, VP-57,
VP-58
VP-6, VP-7
Shear Valve Driver Module
VP-1, VP-56, VP-57, VP-58
VP-5, VP-56, VP-57, VP-58
VP-7, VP-57
Laser Power Supply
VP-46
Optical Flow Cell
Solenoid Driver Module
SDM/CDM
VP-9
Syringe Driver Assembly
VP-6, VP-7, VP-56, VP-57,
VP-58
Peri-Pump Assembly
VP-6, VP-8, VP-7, VP-56,
VP-57, VP-58
Sample Handler Module #1
VP-31, VP-32, VP-33, VP-34
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5-3
8#0#9HULILFDWLRQ#3URFHGXUHV • 5.1 How to Use This Chapter
REPLACED ASSEMBLY
ASSOCIATED VPs
(Perform In Order Listed)
S. Valve Center Ceramic
VP-1, VP-56, VP-57, VP-58
Hard Disk Drive
Floppy Disk Drive
VP-53, VP-54
VP-55
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
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8#0#9HULILFDWLRQ#3URFHGXUHV • VP-1 System Voltage Verification/Adjustment
5.3 Verification Procedures
VP-1 System Voltage Verification/Adjustment
Required Tools & Materials:
Table 5-1
Voltage Specifications
Test Point
Function
Range
Ripple
PSM-TP4
Analog Ground
N/A
N/A
PSM-TP2
+12 VDC UREG
15.0V-19.6V
< 3.0V PP
•
# 2 Phillips Screwdriver
PSM-TP1
+24 VDC UREG
28.9V-37.7V
< 7.0V PP
•
Digital Voltmeter
PSM-TP7
N/A
N/A
N/A
•
Clip Leads
PSM-TP6
N/A
N/A
N/A
•
Potentiometer Adjustment Tool
PSM-TP3
+15 VDC
14.6V-15.4V
< 75MV PP
PSM-TP5
-15 VDC
14.6V-15.4V
< 75MV PP
N/A
-12 VDC
11.50-12.50V
<75MV PP
N/A
+12 VDC
11.50-12.50V
<75MV PP
N/A
+5 VDC
5.00V-5.40V
<75MV PP
N/A
+24 VDC REG
24.0-25.0
<75MV PP
Verification Procedure:
1. Open right side door to gain access to power supply.
2. Refer to Figure 5-1. Connect voltmeter ground lead to TP4
on power supply module.
3. Verify the voltages according to Table 5-1.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
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8#0#9HULILFDWLRQ#3URFHGXUHV • VP-2 A/D Verification/10 Volt Reference Adjustment
Figure 5-1
Power Supply Voltage Testpoints
REAR
TO P
15 A S B (+ 1 2V )
F1
ALARM
T P 1 + 24 V
F2
-12V
T P 2 + 12 V
15 A S B (+ 2 4V )
PSM
+ 12 V
T P 3 + 15 V
TP4
GND
TP5
-15V
+ 24 V
GND
+ 5V
10 0V
TP6 RET
+ 10 0V
TP7
VP-2 A/D Verification/10 Volt Reference
Adjustment
The CELL-DYN 3200 utilizes an A/D converter to convert all
critical voltages levels to a 12-bit digital code. This code is read
by the computer and these voltages are then displayed on the
“VOLTAGE READINGS” screen in “DIAGNOSTICS.” The
following procedure verifies the accuracy of this A/D Converter
by using the A/D to read the 9.901 volt reference generated on
the CPU/DCM and is also used to set the level of the 10 volt
reference.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
3 2 0 76
Required Tools & Materials:
•
# 2 Phillips Screwdriver
•
Digital Voltmeter
•
Clip Leads
•
Potentiometer Adjustment Tool
Verification Procedure:
1. Open right side door to gain access to the CPU/DCM.
Part Number 9140300 − August 1998
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8#0#9HULILFDWLRQ#3URFHGXUHV • VP-3 MAM Reference Voltage Verification/Adjustment
2. Locate the CPU/DCM on the right side door and connect
the positive lead of the DVM to TP1 and the negative lead
to TPAGND.
3. Adjust R4 for a reading of 10.00 volts +/- 0.01V.
4. Move the positive lead to TP3 (9.901V reference) and
verify that the meter reading is 9.90 volts +/- 0.03V.
5. Move the positive lead to TP2 (99mV reference) and verify
that the meter reading is 0.099 volts +/- 0.003V.
6. From Main Menu, press
Verification Procedure:
1. Connect positive lead of DVM to TP2 and negative to TP7.
2. If necessary, adjust R86 for a reading of 10.00 volts +/0.01V.
VP-4 VPM Reference Voltage
Verification/Adjustment
Required Tools & Materials:
„ DIAGNOSTICS
•
# 2 Phillips Screwdriver
„ MORE
•
Digital Voltmeter
„ VOLTAGE READINGS
•
Clip Leads
•
Potentiometer Adjustment Tool
7. Press [VOLTAGE READINGS] and observe the “9.901V
Reference” on the SPM. Verify by repeatedly pressing the
key that the voltage displayed is 9.90 volts +/- 0.05V.
Verification Procedure:
VP-3 MAM Reference Voltage
Verification/Adjustment
1. Open flow panel door to gain access to the VPM.
Required Tools & Materials:
3. If necessary, adjust R16 for a reading of 10.00 volts +/0.01V.
•
# 2 Phillips Screwdriver
•
Digital Voltmeter
•
Clip Leads
•
Potentiometer Adjustment Tool or Small Slotted
Screwdriver
CELL-DYN 3200 SYSTEM SERVICE MANUAL
2. Connect positive lead of DVM to TP2 (VREF+) and
negative to TP1 (VREF-).
Part Number 9140300 − August 1998
5-7
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-5 HGB Current Verification/Adjustment
VP-5 HGB Current Verification/Adjustment
This procedure adjusts the HGB LED current to yield a HGB
output voltage of 5.00 volts with the HGB Flow Cell filled with
Reference Solution.
6. Move the cursor to “HGB CURRENT”; enter new setting,
and press [ENTER]. A change of 50 will yield a voltage
change of approximately 0.3 volts.
7. Press
„ SET ANALYZER
Required Tools & Materials:
•
„ RETURN
None
Verification Procedure:
„ RETURN
8. Press
„ VOLTAGE READINGS
1. From Main Menu, press
and repeat steps 2 through 8 until the voltage is within
specifications.
„ DIAGNOSTICS
„ MORE
„ VOLTAGE READINGS
2. Move the cursor to HGB output, and press
„ SELECT
„ FINISH SELECT
3. Run one background count to insure that the HGB mixing
chamber is full of liquid.
4. Verify that the “HGB OUTPUT” voltage is 5.00 volts
+0.2/-0.00 volts. If the voltage is within range, skip steps 5
through 8.
5. Press
„ MORE
to the first diagnostic screen, and press
„ F12
„ F1
VP-6 Motor Power Verification
This procedure verifies the winding current of the system
stepper motors.
Required Tools & Materials:
•
None
Verification Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ MOTOR OPERATION
2. Press
„ MOTOR PWR CHECKING
and wait for the display.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
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8#0#9HULILFDWLRQ#3URFHGXUHV • VP-7 Motor Operation Verification
3. Verify each motor meets the specifications shown in Table
5-2.
Table 5-2
2. Press
„ EXERCISE MOTORS
type the letter of the suspect assembly, and press
Motor Power Specifications
„ ENTER
Phase 0
Phase 0
Phase 0
Phase 3
Motor
Low
Medium
High
High
A
1.5-2.1
3.4-4.2
4.9-5.9
4.9-5.9
B
.6-1.2
2.5-3.1
4.2-5.0
4.2-5.0
C
.6-1.2
2.5-3.1
4.2-5.0
4.2-5.0
VP-8 Motor Homing Verification
D
.6-1.2
2.5-3.1
4.2-5.0
4.2-5.0
E
1.5-2.1
3.4-4.2
4.9-5.9
4.9-5.9
This procedure allows visual verification of the homing
operation of the system motors by running a homing routine.
G
1.5-2.1
3.4-4.2
4.9-5.9
4.9-5.9
3. Verify that the assembly moves smoothly without stopping
or jerking.
4. Use the [SHEAR VALVE] and [Y VALVE] keys to exercise
the shear valve and Y valve.
Required Tools & Materials:
•
VP-7 Motor Operation Verification
This procedure allows visual verification of the operation of the
system motors by running an exercise routine.
None
Verification Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
Required Tools & Materials:
•
None
Verification Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ MOTOR OPERATION
CELL-DYN 3200 SYSTEM SERVICE MANUAL
„ MORE
„ MOTOR OPERATION
2. Press
„ HOME MOTORS
type the letter of the suspect assembly, and press
„ ENTER
3. Verify that the assembly moves smoothly without stopping
or jerking.
Part Number 9140300 − August 1998
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8#0#9HULILFDWLRQ#3URFHGXUHV • VP-9 Solenoid Bank Verification
VP-9 Solenoid Bank Verification
This procedure allows visual verification of the operation of a
bank of eight solenoids by running an exercise routine.
Verification Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
Required Tools & Materials:
•
None
„ SOLENOID OPERATION
2. Press
„ STEP SOLENOID
Verification Procedure:
type the number of the starting solenoid, and press
1. From Main Menu, press
„ ENTER
„ DIAGNOSTICS
„ MORE
„ SOLENOID OPERATION
2. Press
„ CYCLE BANK
type the number of the bank, and press
„ ENTER
3. Use the “UP/DOWN ARROW KEYS”, and verify that the
solenoids are opened when commanded.
VP-11 Bar Code Reader Verification/Alignment
Required Tools & Materials:
•
Specimen Tube With Bar Code Label
3. Verify that the solenoids are opened in ascending order.
Verification Procedure:
VP-10 Individual Solenoid Verification
This procedure allows visual verification of the operation of a
bank of eight solenoids by running an exercise routine.
Required Tools & Materials:
•
None
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ BAR CODE ALIGNMENT
2. Record the tube bar code number, and place tube at the
read station.
3. Press
„ BAR CODE ALIGNMENT
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 10
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-12 Specimen Sensor #2 Verification/Adjustment
4. Verify that the number displayed on the screen matches
the recorded number, and the read rate is constant.
5. If necessary, loosen the locking screws, and align the
position of the reader until the read rate is constant.
6. Tighten the locking screws and re-verify.
VP-12 Specimen Sensor #2
Verification/Adjustment
Required Tools & Materials:
•
CELL-DYN 3200 Diluent/Sheath
•
Low Abnormal Control
•
DVM with Clip Leads
•
Potentiometer Adjustment Tool
Verification Procedure:
1. Remove top and top right covers.
2. Run controls and insure that results are within
specifications.
3. Create a specimen dilution using LOW ABNORMAL
CONTROL material and CELL-DYN 3200 Diluent/Sheath
that gives an RBC value of 0.8MEG/ul, +/- .03.
4. Connect the positive lead of the DVM to TP1 and the
negative lead to TP3 on the specimen sensor board.
NOTE: The voltage at TP4 should be <0.50V when air or
liquid is passing through the sensor, and >3.00V with the
diluted control.
7. Run a background count, and verify a reading of (0.50V.
8. While observing the tubing through specimen sensor #2,
place the diluted low control under the open probe, and
manually open valve #12 until the dilution is aspirated
approximately 2 inches past specimen sensor #2.
9. Verify a reading of >3.00V.
10. Manually open solenoid 13 to remove blood from lines,
and run a background count.
11. Run another background count, and verify that the LED on
the sensor board does not flicker.
12. Disconnect the DVM from the specimen sensor board.
VP-13 LSI Loopback Verification
Required Tools & Materials:
•
Loopback Connector PN 9211532
Verification Procedure:
1. Turn power off, install loopback connector at COM1 (top
serial connector) on PC module, and power-up and
initialize instrument.
5. Adjust R1 for a reading of 2.00 +/-0.01 volts.
6. Move positive lead to TP4.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
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8#0#9HULILFDWLRQ#3URFHGXUHV • VP-14 MAM & SPM Signal Generator Verification
2. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ SERIAL TEST
3. Press
„ TRANSMIT MESSAGE
and verify that the message received matches the
message sent: “CELL-DYN serial interface test.”
4. Press
„ STOP TRANSMISS
„ RETURN
„ RETURN
5. Turn power off, and remove loopback connector.
VP-15 Vacuum & Pressure Retention
Verification
This procedure tests the ability of the system to retain the
appropriate vacuum and pressure levels. A failure indicates a
leak in the system.
Required Tools & Materials:
•
None
Verification Procedure:
1. Ensure that the instrument is in the “READY” mode.
2. Verify that off-time of the vacuum pump is 2 minutes or
greater between automatic recovery cycles.
3. Verify that off-time of the pressure pump is 2 minutes or
greater between automatic recovery cycles.
VP-14 MAM & SPM Signal Generator
Verification
Required Tools & Materials:
•
None
Verification Procedure:
•
Not Available At This Time
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 12
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-16 Vacuum & Pressure Level Verification/Adjustment
VP-16 Vacuum & Pressure Level
Verification/Adjustment
to the first diagnostic screen and press
„ F12
„ F1
Required Tools & Materials:
•
Use the following equation to calculate the new setting(s).
None
New Setting = Target Level X Current Setting
Actual Level
Verification Procedure:
1. Ensure that the instrument is in the “Open Sampler” mode.
6. Move the cursor to the appropriate vacuum(s) or
pressure(s), enter new setting(s) and press
„ ENTER
2. From Main Menu, press
„ DIAGNOSTICS
7. Press
„ MORE
„ SET ANALYZER
„ VOLTAGE READINGS
„ RETURN
3. Observe the vacuum and pressure levels and compare
with Table 5-3.
Table 5-3
Vacuum & Pressure Specifications
Vacuum/Pressure
Specification
Pressure #1
12.5-13.5 psi
Pressure #2
8.5-9.5 psi
Pressure #3
4.0-4.5 psi
Vacuum #1
12.5-13.5” Hg
Vacuum #2
3.1-3.5” Hg
4. If all the vacuum and pressure levels are within
specifications, do not perform steps 5 through 8.
„ RETURN
NOTE: If the level is reduced the vacuum or pressure
must be bled off to the new level before performing step 8.
A count cycle may be used for bleeding.
8. Go to “VOLTAGE READINGS”, and repeat steps 3 through
8 until the level(s) are within specifications.
VP-17 Vacuum & Pressure Recovery
Verification
This procedure tests the ability of the vacuum and pressure
pumps to return from atmosphere to the appropriate levels. A
failure indicates that the pump under test is defective or there is
a gross leak in the system.
5. Press
„ MORE
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 13
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-18 Laser Replacement/Alignment Procedure
Required Tools & Materials:
•
None
Verification Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
to VACUUM TEST and PRESSURE TEST.
2. Press
„ VACUUM TEST
and verify that the elapsed time is 4.5 seconds or less.
3. Press
„ PRESSURE TEST
and verify that the elapsed time is 3.5 seconds or less.
VP-18 Laser Replacement/Alignment
Procedure
'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.
Required Tools & Materials:
•
# 2 Phillips Screwdriver
•
.050 Allen Wrench
•
Optics Alignment Kit Cat. #8921183801
•
DVM with Clip Leads
•
Laser Power Meter
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Verification Procedure:
1. Ensure instrument power is off. Remove top cover, both
optics covers, and open flow cell access door.
'$1*(5=#+,*+#92/7$*(#Assure the power
to the instrument is off. A high voltage charge may
remain on the laser connector with the power off.
Do not touch the male pins. Disconnect the laser
connector and short both male pins to the
instrument chassis.
2. Disconnect laser connector and short both male pins to
instrument chassis.
3. Remove Laser Clamps and replace Laser.
4. Replace clamps and tighten until snug but Laser can still
be moved.
5. Move laser body until 3/4 inch extends beyond front laser
clamp and rotate laser body until front label is on top.
6. Connect laser connector, ensure laser shutter is closed,
turn on power, and prime instrument. Allow a 15-minute
laser warm-up period.
7. Refer to Figure 5-4. Mount Power Meter in beam path.
Open Laser Shutter and verify a reading of no less
than 5 mW.
8. If you have a polarizing filter, skip step 9.
9. Scribe optics bench on right and front sides of beam
splitter/polarizer assembly, and remove assembly from
90o path.
Part Number 9140300 − August 1998
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8#0#9HULILFDWLRQ#3URFHGXUHV • VP-18 Laser Replacement/Alignment Procedure
10. Place polarizing filter or beam splitter/polarizer in beam
path between laser and power meter with beam splitter
facing laser.
11. While observing power reading, rotate the laser body
clockwise. As power level decreases, change scales as
needed.
12. Rotate laser body until power is at minimum on the 20 uW
scale.
13. Tighten laser clamps and verify that power level does not
change more than +/- 3 uw and remove beam
splitter/polarizer from beam path. Laser is now aligned for
true vertical polarization.
repeated several times to attain proper alignment. Record
power level through slit and, if necessary, replace beam
splitter/polarizer in normal position.
19. Connect the negative lead of the DVM to TP2 and the
positive lead to TP1 on the 0o photodiode PCB.
20. Refer to Figure 5-2. Manually open solenoid 56 and
remove tubing from port #1 on the flow cell distribution
manifold.
Figure 5-2
Flow Cell Plumbing.
5
14. Place alignment post (#9420071) in front tooling hole and
center beam on hole using X and Y alignment screws on
rear mirror. if alignment post is not available, center beam
on cylindrical lens. Placing scotch tape on rear of
cylindrical lens will aid in centering beam.
56
WBC
FLO W
C EL L
91
3
FLO W C E LL M AN IFO LD
15. Remove alignment post and place power meter behind
forward slit.
16. Use X alignment (.050" allen wrench) screw on rear mirror
to obtain maximum power through forward slit. Maximum
power through slit should be approximately 1/5 of laser
power.
17. Use X alignment screw on front mirror to move beam
toward alignment hole in center of obscuration bar.
18. Repeat steps 16 through 18 until maximum power is being
transmitted through slit and beam is centered horizontally
on hole in center of obscuration bar. This may have to be
CELL-DYN 3200 SYSTEM SERVICE MANUAL
4
54
1
57
2
52
32 077
1
2
3
4
5
21. Observe the 90o slit. When two red images of the flow cell
walls appear on the slit, close solenoid 56 and reconnect
tubing to port #1.
22. Adjust the Y alignment screw on the front mirror for a
minimum reading on the DVM. This reading should be less
than 0.300 volts.
23. Adjust the Y alignment screw on the rear mirror until the
red images are in the center 1/3 of the 90o slit.
Part Number 9140300 − August 1998
5 - 15
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-19 Front/Rear Mirror Replacement/Alignment Procedure
24. Repeat steps 22 and 23 until the red images are in the
center 1/3 of the slit and the DVM voltage is at minimum.
The minimum voltage should be less than 0.200 volts.
25. Re-check power transmitted through forward slit. If it has
changed, a slight X alignment of the rear mirror is
necessary.
26. Replace both optics covers.
2. Remove, replace, and secure defective mirror (front
or rear).
3. Place alignment post (#9420071) in front tooling hole and
center beam on hole using X and Y alignment screws on
rear mirror.
4. Remove alignment post and place power meter behind
forward slit.
27. Perform Optical Flow Cell Verification/Fine Alignment
VP-20 and, if necessary, perform Optical Flow Cell Coarse
Alignment VP-21, and repeat VP-20.
5. Use X alignment (.050" allen wrench) screw on rear mirror
to obtain maximum power through forward slit. Maximum
power through slit should be approximately 1/5 of laser
power.
VP-19 Front/Rear Mirror
Replacement/Alignment Procedure
6. Use X alignment screw on front mirror to move beam
toward alignment hole in center of obscuration bar.
'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.
Required Tools & Materials:
•
# 2 Phillips Screwdriver
•
.050 Allen Wrench
•
Optics Alignment Kit Cat. #8921183801
•
DVM with Clip Leads
•
Laser Power Meter
7. Repeat steps 4 through 6 until maximum power is being
transmitted through slit and beam is centered horizontally
on hole in center of obscuration bar. This may have to be
repeated several times to attain proper alignment. Record
power level through slit and, if necessary, replace beam
splitter/polarizer in normal position.
8. Connect the negative lead of the DVM to TP2 and the
positive lead to TP1 on the 0o photodiode PCB.
9. Refer to Figure 5-3. Manually open solenoid 56 and
remove tubing from port #1 on the flow cell distribution
manifold.
Verification Procedure:
1. Remove top cover, both optics covers, and open flow cell
access door.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 16
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-20 Optical Flow Cell Verification/Fine Alignment
5
VP-20 Optical Flow Cell Verification/Fine
Alignment
56
'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.
WBC
FL O W
CELL
91
3
FL O W C E LL M A NIFO L D
4
57
54
1
2
52
32077
Figure 5-3
1
2
3
4
5
Flow Cell Plumbing
10. Observe the 90o slit. When two red images of the flow cell
walls appear on the slit, close solenoid 56 and reconnect
tubing to port #1.
11. Adjust the Y alignment screw on the front mirror for a
minimum reading on the DVM. This reading should be less
than 0.300 volts.
12. Adjust the Y alignment screw on the rear mirror until the
red images are in the center 1/3 of the 90o slit.
13. Repeat steps 11 and 12 until the red images are in the
center 1/3 of the slit and the DVM voltage is at minimum.
The minimum voltage should be less than 0.200 volts.
14. Re-check power transmitted through forward slit. If it has
changed, a slight X alignment of the rear mirror is
necessary.
The alignment of the optical flow cell is one of the most critical
alignments performed on the CELL-DYN 3200. It must be
optimized so that the instrument can properly perform the WBC
5-Part Differential measurements. However, if this alignment is
not really needed, it is best that it not be performed. Therefore,
if the instrument is within the prescribed specifications, do not
attempt to align the optical flow cell.
Required Tools & Materials:
•
# 2 Phillips Screwdriver
•
7.00 µ Diameter Beads, PN 1606004
•
CELL-DYN 3200 Diluent/Sheath
•
Clean Container
Verification Procedure:
1. Prepare the WBC Beads solution.
a. Place 2mL of diluent in a clean container.
b. Add 15 drops of 7.00 µ beads and mix well.
2. From Main Menu, press
15. Replace both optics covers.
„ DIAGNOSTICS
16. Perform Optical Flow Cell Verification/Fine Alignment
VP-20 and, if necessary, perform Optical Flow Cell Coarse
Alignment VP-21, and repeat VP-20.
„ MORE
CELL-DYN 3200 SYSTEM SERVICE MANUAL
„ WBC DATA
Part Number 9140300 − August 1998
5 - 17
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-20 Optical Flow Cell Verification/Fine Alignment
7. Loosen the locking screw on the lower (y-axis)
micrometer, and cover the opening with black cloth to
block ambient light.
3. Press
„ CALC CV
4. Place Beads solution under probe and press “TOUCH
PLATE” to aspirate Beads and run a count cycle.
5. Refer to Table 5-4 and compare the CVs to those
displayed on the screen. If the displayed CVs are less than
those from the table, the flow cell does not need
alignment.
NOTE: If any or all of the CVs are out-of-specification, the
flow cell must be completely aligned. The 0o and 10o
channels must be aligned first by adjusting the Y-axis
alignment (lower) micrometer and then the 90o and 90o
depolarized must be aligned by adjusting the X-axis
alignment (right) micrometer.
Table 5-4
WBC Mean Channel & CV Specification
Channel
Mean
Range
CV
0o
35
32-38
<3.5%
10o
65
60-70
<3.5%
90o
* Derived From Opti-Cal +/- 5 Channels <12.5%
o
90 Dep. * Derived From Opti-Cal +/- 5 Channels <12.5%
NOTE: * These values must be obtained from the
Beads auto-gain default table, or by performing the
Opti-Cal procedure.
6. Open the flow cell access door, located at the top left of
the Analyzer.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
8. Place the 7.00 µ beads solution under the probe, and
press
„ EXTENDED WBC COUNT
„ START
to aspirate the Beads and start the cycle.
9. Observe the 0o and 10o display on the left side of the
screen. When dots appear on the screen, adjust the lower
micrometer until the tightest possible grouping of dots and
lowest possible CVs are attained. If the cycle stops before
this is accomplished, repeat step 8 and try again.
10. Place Beads solution under probe and “START SWITCH”
to aspirate Beads and run a count cycle.
11. Press
„ CALC CV
when the cycle is completed. If the 0o and 10o CVs are
within specifications, continue with the procedure. If not,
repeat steps 8 through 10.
NOTE: If repeated alignment does not bring the CVs
within specifications, further troubleshooting is required to
isolate the problem. The first considerations are a
contaminated or defective flow cell or a defective laser.
The last consideration should be that of a misaligned
optical bench, since once properly aligned and secured,
none of the assemblies should move under normal
conditions.
Part Number 9140300 − August 1998
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8#0#9HULILFDWLRQ#3URFHGXUHV • VP-20 Optical Flow Cell Verification/Fine Alignment
12. Loosen the locking screw on the right (x-axis) micrometer,
and cover the opening with black cloth to block ambient
light. Then press the “START” key to aspirate the Beads
and start the cycle.
13. Observe the 90o and 90o depolarized display on the right
side of the screen. When dots appear on the screen,
adjust the micrometer to attain the greatest deflection to
the right and up. CVs are not as important as in the 0o and
10o channels.
14. Place Beads solution under probe and press “START
SWITCH” to aspirate Beads and run a count cycle.
15. Press the key when the cycle is completed. If the 90o and
90o depolarized CVs are within specifications, the
procedure is completed. If not, repeat steps 12 through 15.
16. Tighten the locking screws and record the setting for future
reference.
17. Perform PMT Dynode Voltage Verification/Adjustment
VP-22.
18. Perform WBC Gain Verification/Adjustment VP-23.
19. Perform WBC OPTI-CAL Verification/Adjustment (VP-24).
20. Perform NOC Gain Adjustment VP-25.
21. Perform RBC/PLT 0o Gain Verification/Adjustment VP-26.
22. Perform RBC/PLT 10o Gain Verification/Adjustment
VP-27.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 19
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-20 Optical Flow Cell Verification/Fine Alignment
Figure 5-4
CELL-DYN 3200 Optics Bench
R ear M irror
X A xis
Laser S trap
Laser S trap
5 M illiw att H E N E Laser
Vertical P olarization
632.8 N anom eters
90 oD epolarized P M T
.75"
P lace B e am splitter/
P olarizer here during
Laser po larization.
90 oP M T
B eam sp litter/P olarizer
Y
X
Y
P lace P o w er M eter
H ead he re during
Laser po larization.
A
x
i
s
90 oS lit/F ield Lens
M irror R e ar View
F ixture H oles
90 oO bjective Lens
10 o D etector
C ylinderc al Lens
P erforate d M irror M ount
Tooling
H ole
F orw ard S lit
Im aging Lens
0 oD etector
F low C ell
Y -A xis A lignm ent M ic.
F ront M irror
CELL-DYN 3200 SYSTEM SERVICE MANUAL
O bscura tion B ar
X -A xis A lignm ent M ic.
Part Number 9140300 − August 1998
F orw ard S catter A ssy.
3 2 078
5 - 20
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-21 Optical Flow Cell Coarse Alignment
VP-21 Optical Flow Cell Coarse Alignment
'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.
Required Tools & Materials:
•
7.00 µ Diameter Beads
•
CELL-DYN 3200 Diluent/Sheath
•
Clean Container
5. Continue rotating CCW until the pattern of the front wall is
observed, and record the micrometer setting (Figure
5-5-C).
Figure 5-5
Beam Images
"B "
R ear W all
"A "
C lear Q u artz
S catter P attern
O bscuration B ar
"C "
F ront W all
"D "
F low C ell C entered
Verification Procedure:
1. Prepare the Bead solution.
320 79
B rightest Im age
a. Place 2mL of diluent in a clean container.
b. Add 15 drops of 7.00 µ beads and mix well.
2. Remove top cover and flow cell access cover.
3. Loosen the locking screw on the lower (y-axis)
micrometer, and back off (CW viewed from top) on the
micrometer (Figure 5-5) until the beam is striking the flow
cell at a point to the rear of the flow cell channel. The
scatter pattern on the obscuration bar should resemble
that in Figure 5-5-A. Placing a piece of white paper in front
of the obscuration bar will aid in viewing the scatter
pattern.
4. While observing the scatter pattern, rotate the micrometer
CCW until the pattern of the rear wall of the flow cell
channel is observed, and record the micrometer setting
(Figure 5-5-B).
CELL-DYN 3200 SYSTEM SERVICE MANUAL
6. Rotate the micrometer CW to a setting half-way between
those recorded in steps 3 and 4. This should coarsely
center the flow cell channel on the beam; the pattern will
resemble that of Figure 5-5-D and will be at the brightest
level.
7. Cover the opening with black cloth to block ambient light.
8. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ WBC DATA
9. Press
„ CALC CV
Part Number 9140300 − August 1998
5 - 21
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-22 PMT Dynode Voltage Verification/Adjustment
10. Place Beads solution under probe and press “START
SWITCH” to aspirate Beads and run a count cycle.
11. If there is data in both displays, perform the fine alignment
procedure VP-20. If there is no data in either display,
Repeat steps 5 through 9.
2. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ WBC DATA
3. Press
VP-22 PMT Dynode Voltage
Verification/Adjustment
'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.
Required Tools & Materials:
•
# 2 Phillips Screwdriver
•
3/16” Allen Wrench
•
7.00 µ Diameter Beads, PN 1606004
•
CELL-DYN 3200 Diluent/Sheath
•
Clean Container
Verification Procedure:
1. Prepare the WBC Beads solution:
„ CALC CV
4. Place Beads solution under probe and press “START
SWITCH” to aspirate Beads and run a count cycle.
5. Observe the mean channel values for the 90o and 90o
depolarized channels, and refer to Table 5-4. If the
displayed mean channel values are within specifications
and the recorded gain settings are less than 2500, the
dynode voltages need not be adjusted and the procedure
is completed. If either of the above are out-of-range, the
dynode voltage(s) for the channel(s) must be adjusted.
6. Remove the top cover from the analyzer, and locate the
PMT preamplifiers on the right side of the optical bench.
The board on the left is for the 90o channel and the board
on the right is for the 90o depolarized channel. R11 adjusts
the dynode voltage on both boards.
7. Press
„ MORE
a. Place 2 mL of diluent in a clean container.
to first Diagnostics Screen, and press
b. Add 15 drops of 7.00 µ beads and mix well.
„ F12
„ F1
„ SET POINT ENTRY
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 22
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-23 WBC Gain Verification/Adjustment
8. Move cursor to “WBC 90D”, enter 2000, and press
“ENTER.” Move cursor to “WBC 90DP”, enter 2000, and
press “ENTER.”
14. Press
„ DIAGNOSTICS
„ MORE
9. Press
„ SET ANALYZER
„ RETURN
„ RETURN
„ MORE TO
„ WBC DATA
10. Cover optical bench with black cloth to block ambient light.
11. Place the 7.00 µ beads solution under the probe, and
press
„ EXTENDED WBC COUNT
„ START
to aspirate the Beads and start the cycle.
o
NOTE: Both 90 channels are extremely sensitive to
ambient light. The black cloth must remain in place when
adjusting the dynode voltages.
12. Observe mean channel value on right display and adjust
R11 on 90o preamplifier (left PCB) until the displayed
values approximate the specifications shown in Table 5-1.
(Note that this is a coarse preliminary manual adjustment
that will be more finely tuned with the auto gain circuitry.)
13. Repeat steps 11 and 12 for the 90o depolarized
preamplifier (right PCB).
CELL-DYN 3200 SYSTEM SERVICE MANUAL
„ VOLTAGE READINGS
15. Verify that both dynode voltages are less than 9.00 (900
volts). Dynode voltages greater that 900 volts indicate a
problem, and the problem must be isolated and corrected
before continuing.
VP-23 WBC Gain Verification/Adjustment
The following procedure verifies, and if necessary, adjusts the
gain of the WBC channels using 7.00 µ beads as a reference
material.
There are two modes available for adjusting the WOC gain “ENTER SETTING” and “AUTO GAIN ADJUSTMENT.”
In the “ENTER SETTING” mode the proper gain setting is
calculated manually and then entered. Use the following
equation when using this mode.
New Gain Setting =
Desired Mean Channel
Present Mean Channel
X Present Setting
In the “AUTO GAIN ADJUSTMENT” mode Beads is run on the
analyzer and the new gain setting is calculated and entered
automatically. We will use this mode, if necessary, in the
following procedure.
Required Tools & Materials:
•
7.00 µ Diameter Beads
•
CELL-DYN 3200 Diluent/Sheath
Part Number 9140300 − August 1998
5 - 23
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-24 WBC OPTI-CAL Verification/Adjustment
•
Clean Container
Verification Procedure:
1. Prepare the WBC Beads solution:
a. Place 2mL of diluent in a clean container.
b. Add 15 drops of 7.00 µ beads and mix well.
2. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ GAIN ADJUSTMNTS
3. Press
„ VERIFY GAINS
„ WBC GAINS
4. If necessary, change the Mean Channel Numbers to the
desired values (Table 5-1) and press
„ ENTER
5. Place Beads solution under probe, press
„ START
and press the “START SWITCH” to aspirate Beads and
run a count cycle.
6. If the Mean Channel Numbers are within specifications,
skip steps 7 through 12.
7. Press
„ RETURN
to access “AUTO GAIN ADJUSTMNT
CELL-DYN 3200 SYSTEM SERVICE MANUAL
8. Press
„ AUTO GAIN ADJUSTMNT
„ WBC GAINS
9. If necessary, change the Mean Channel Numbers to the
desired values (Table 5-1) and press “ENTER.”
NOTE: You must run Beads twice in order to set gains.
The first run is needed to calculate new settings, and the
second is to verify the settings.
10. Place Beads solution under probe, press
„ START
and press the “START SWITCH” to aspirate Beads and
run a count cycle.
11. Place Beads solution under probe and press “START
SWITCH” to aspirate Beads and run a count cycle to
verify.
12. Press
„ Y
„ ENTER
to save new gain settings.
VP-24 WBC OPTI-CAL Verification/Adjustment
The following procedure verifies, and if necessary, adjusts the
gain of the WBC 90o and 90oD channels using OPTI-CAL as a
reference material. It also determines and stores the Beads
reference channels to be used for reference after an OPTI-CAL
gain adjustment.
Part Number 9140300 − August 1998
5 - 24
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-24 WBC OPTI-CAL Verification/Adjustment
Required Tools & Materials:
9. Press
•
7.00 µ Diameter Beads, PN 1606004
•
CELL-DYN 3200 Diluent/Sheath
•
Clean Container
10. Change window settings to conform to the OPTI-CAL
assay sheet.
•
OPTI-CAL
11. Press
„ CELL REGION
„ RETURN”
Verification/Adjustment Procedure:
12. Toggle the “BLOOD/BEADS” key to blood.
1. Insure Channels 1 through 4 offset voltages are less than
2 volts.
13. Run the OPTI-CAL 3 times and record the mean channel
values for GRANs on 90o and 90oD on each run.
2. Insure Laser power is greater than 5mw and less than
7mw.
14. Compute the average mean channel for 90o and 90oD for
the three runs.
3. Insure Channel WOC gain settings are less than 2500.
15. Compare the average to the Gran mean channel location
given in the OPTI-CAL assay sheet. If it is within
specifications, skip steps 16 through 20.
4. Insure Dynode voltages are less than 900 volts.
5. Insure the CVs on all 4 channels are: 0o < 3.5%, 10o <
3.5%, 90o < 12.5%, 90oD < 12.5%.
6. Insure the Auto Gain function has been run and the gains
have been set to the following:
0o = 35 +/- 3, 10o = 65 +/- 5.
7. From Main Menu, press
16. Calculate the new gain values for 90o and 90oD, use the
following equation:
X (Y/Z)
Where:
X = Current Gain Value.
„ DIAGNOSTICS
Y = Mean Channel Value from Assay Sheet.
„ F12
Z = Current Mean Channel from Step 14.
„ F1
8. Press
„ BEADS/BLOOD COMPARE
CELL-DYN 3200 SYSTEM SERVICE MANUAL
17. Press
„ RETURN
„ SET POINT ENTRY
Part Number 9140300 − August 1998
5 - 25
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-25 NOC Gain Adjustment
18. Enter the new value for 90o gain and press
26. Press
„ MORE
„ ENTER
„ GAIN ADJUSTMNTS
19. Enter the new value for 90oD gain and press
„ ENTER
27. Press
„ SET ANALYZER
„ AUTO GAIN ADJUSTMNT
„ RETURN
„ WBC GAINS
20. Press
„ BEADS/BLOOD COMPARE
and repeat steps 12 through 15.
21. Toggle “BLOOD/BEADS” key to BEADS.
22. Prepare the Bead solution.
a. Place 2mL of diluent in a clean container.
b. Add 15 drops of 7.00 µ beads and mix well.
23. Run the Beads three times and record the Mean Channel
for 90o and 90oD on each run. Verify that 0o and 10o Mean
Channels are 35+/-3 and 65+/-5 respectively and all CVs
are within specification on each run.
24. Compute the average Mean Beads Channel for 90o and
90oD for the three runs.
NOTE: These Beads Channel locations are now specific
to this analyzer and must be entered into the AUTO GAIN
table.
28. Enter the Beads Channel numbers for 90o and 90oD.
29. Run an Auto Gain using Beads to enter these channel
locations into the default table.
30. Reboot system on a Setup Disk. Perform a “GET” to store
these values.
VP-25 NOC Gain Adjustment
The NOC gains are not calibrated with beads because the 0o
and 10o channels are used for both WOC and NOC counting.
However, the NOC gains must be higher than those used for
WOC by a factor of 1.5. In this procedure, we simply multiply
the WOC 0o and 10o gains by 1.5 and change the NOC 0o and
10o setpoints to the new values.
Required Tools & Materials:
•
None
25. Press
„ RETURN
„ RETURN
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 26
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-26 RBC/PLT 0° Gain Verification/Adjustment
Verification/Adjustment Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
„ F12
„ F1
2. Press
„ SET POINT ENTRY
VP-26 RBC/PLT 0° Gain
Verification/Adjustment
The following procedure verifies and, if necessary, adjusts the
gain of the RBC/PLT 0o channel using Beads spheres as a
reference material.
Required Tools & Materials
•
7.00 µ Diameter Beads, PN 1606004
3. Move the cursor to the NOC 0o setpoint.
•
CELL-DYN 3200 Diluent/Sheath
4. Multiply the displayed WOC 0o setpoint by 1.5, change the
NOC 0o setpoint to the new calculated value, and press
•
Red-Top Vacutainer
•
Transfer Pipette
„ ENTER
5. Move the cursor to the NOC 10o setpoint.
6. Multiply the displayed WOC 10o setpoint by 1.5, change
the NOC 10o setpoint to the new calculated value, and
press
„ ENTER
7. If necessary, move the cursor to the NOC Lo threshold,
change it to 400, and press
„ ENTER
8. Press
„ SET ANALYZER
to store the new values.
9. Press
„ RETURN
Verification/Adjustment Procedure:
1. Prepare the 7.00 µ beads solution:
a. Mix the 7.00 µ beads by shaking vigorously.
b. Add 15 drops of 7.00 µ beads to a vacutainer.
c. Add 2 ml of 3200 Diluent/Sheath reagent.
2. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ GAIN ADJUSTMNTS
3. Press
„ VERIFY GAINS
„ RBC/PLT 0O GAIN
„ RETURN
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 27
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-27 RBC/PLT 10° Gain Verification/Adjustment
4. If necessary, change the Mean Channel Number to the
desired value and press
„ ENTER
5. Place Beads solution under probe, press
11. Place Beads solution under probe and press “START
SWITCH” to aspirate Beads and run a count cycle to
verify.
12. Press
„ START
„ Y
and press the “START SWITCH” to aspirate Beads and
run a count cycle.
to save new gain setting.
6. If the Mean Channel Number is within specifications (180
+/- 1), skip steps 7 through 12.
7. Press
„ RETURN
to access “AUTO GAIN ADJUSTMNT.”
„ ENTER
VP-27 RBC/PLT 10° Gain
Verification/Adjustment
The following procedure verifies and, if necessary, adjusts the
gain of the RBC/PLT 10o channel using Beads spheres as a
reference material.
8. Press
„ AUTO GAIN ADJUSTMNT
„ RBC/PLT 0O GAIN
9. If necessary, change the Mean Channel Number to the
desired value and press
„ ENTER
NOTE: You must run Beads twice in order to set gain. The
first run is needed to calculate new setting, and the second
is to verify the setting.
10. Place Beads solution under probe, press
„ START
and press the “START SWITCH” to aspirate Beads and
run a count cycle.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Required Tools & Materials:
•
3.29 or 3.31 Beads, PN 1606005
•
CELL-DYN 3200 Diluent/Sheath
•
Red-Top Vacutainer
•
Transfer Pipette
Verification/Adjustment Procedure:
1. Prepare the 3.29 or 3.31 Beads solution.
a. Mix the Beads by shaking vigorously.
b. Add 10 drops of Beads and 20 drops of diluent to a
vacutainer and mix well.
Part Number 9140300 − August 1998
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8#0#9HULILFDWLRQ#3URFHGXUHV • VP-28 MAM 0° & 10° Log Amplifier Verification
2. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ GAIN ADJUSTMNTS
3. Press
„ VERIFY GAINS
„ RBC/PLT 10O GAIN
4. If necessary, change the Mean Channel Number to the
desired value and press
„ ENTER
5. Place Beads solution under probe, press
NOTE: You must run Beads twice in order to set gain. The
first run is needed to calculate new setting, and the second
is to verify the setting.
10. Place Beads solution under probe, press
„ START
and press the “START SWITCH” to aspirate Beads and
run a count cycle.
11. Place Beads solution under probe and press “START
SWITCH” to aspirate Beads and run a count cycle to
verify.
12. Press
„ Y
„ START
„ ENTER
and press the “START SWITCH” to aspirate Beads and
run a count cycle.
to save new gain setting.
6. If the Mean Channel Number is within specifications
channel (120 +/- 1 for 3.29 or 121+/- 1 for 3.31), skip steps
7 through 12.
7. Press
„ RETURN
to access “AUTO GAIN ADJUSTMNT.”
8. Press
„ AUTO GAIN ADJUSTMNT
„ RBC/PLT 10O GAIN.”
9. If necessary, change the Mean Channel Number to the
desired value and press
VP-28 MAM 0° & 10° Log Amplifier Verification
The following procedure verifies the dynamic range of the 0o
and 10o log amplifiers on the MAM by running the 3 Beads and
calculating the ratio of the peak channels of the three sizes.
The CELL-DYN 3200 uses the 0o channel for and MCV, and the
10o channel for RBCs, PLTs, and MPV, with the log amplifiers
used for both outputs. The 0o channel gain is set with 7.00 µ
beads by placing the peak channel in 180 +/- 1, and the lower
range is verified with a ratio to a 4.99 RBC 0o Bead peak
channel number. The 100 gain is set with 3.31 or 3.29 Beads by
placing the 3.29 peak channel in 120 +/- 1 or the 3.31 peak
channel in 121 +/- 1, and the upper range is verified with a ratio
to a 7.00 µ beads RBC 10o peak channel number.
„ ENTER
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 29
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-28 MAM 0° & 10° Log Amplifier Verification
The following table and equations are an example of a typical
RBC and PLT verification/adjustment.
Table 5-5
•
Transfer Pipette
Verification/Adjustment Procedure:
0o & 10o Log Amplifier Verification
Beads
RBC 0o
P.C.
RBC 10o
P.C.
PLT 0o P.C.
PLT 10o P.C.
3.29
N/A
N/A
N/A
Set at 120
4.99
Falls at
129
N/A
N/A
N/A
7.00
Set at
180
Falls at 200
N/A
N/A
1. Prepare the 7.00 µ beads solution:
a. Mix the 7.00 µ beads by shaking vigorously.
b. Add 15 drops of 7.00 µ beads to a vacutainer.
c. Add 2 ml of 3200 Diluent/Sheath reagent.
2. In the “RUN” menu, press
„ SPECIMEN TYPE
„ BEADS
„ RETURN.”
0o Equation:
7.00 RBC 0o P.C.
4.99 RBC 0o P.C.
3. From Main Menu, press
= 1.40±0.04
180 = 1.39 (within spec)
129
„ DIAGNOSTICS
„ MORE
„ RBC DATA
o
10 Equation:
7.00 RBC 10o P.C. = 1.67±0.04
3.xx PLT 10o P.C.
200 = 1.66 (within spec)
120
Required Tools & Materials:
•
7.00 µ Diameter Beads, PN 1606004
•
3.29 or 3.31 µ Beads, PN 1606005
•
4.99 µ Beads, PN 1606003
•
CELL-DYN 3200 Diluent/Sheath
•
Red-Top Vacutainer
CELL-DYN 3200 SYSTEM SERVICE MANUAL
4. Turn smoothing off, place Beads solution under probe,
and press the “START SWITCH” to aspirate Beads and
run a count cycle.
5. When cycle is completed, view “RBC 0 DATA” and “RBC 0
HISTOGRAM”, and verify the peak is in channel 180 +/- 1
channel. If the RBC 0o peak channel number is within
specifications, record the RBC 0o and RBC 10o peak
channel numbers, and skip step 6.
6. Perform RBC/PLT 0o Gain Verification/Adjustment
(VP-26), and repeat steps 3 through 5.
7. Prepare the 4.99 µ beads solution:
a. Mix the 4.99 µ beads by shaking vigorously.
Part Number 9140300 − August 1998
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8#0#9HULILFDWLRQ#3URFHGXUHV • VP-28 MAM 0° & 10° Log Amplifier Verification
b. Add 15 drops of 4.99 µ beads to a vacutainer.
8. Place the Beads under probe, and press the “START
SWITCH” to aspirate Beads and run a count cycle.
9. When cycle is completed, view “RBC 0 DATA” and “RBC 0
HISTOGRAM” and record the RBC 0o peak channel
number
10. Prepare the 3.29 or 3.31 Beads solution:
a. Mix the Beads by shaking vigorously.
b. Add 10 drops of Beads and 20 drops of diluent to a
vacutainer and mix well.
11. Press
„ DIAGNOSTICS
„ PLT DATA
„ PLT 10 DATA.”
12. Turn smoothing off, place Beads solution under probe,
and press the “START SWITCH” to aspirate Beads and
run a count cycle.
15. Plug the appropriate 0o peak channel numbers into the
following equation, and calculate the 0o log amplifier ratio.
If the ratio is out-of-specifications, the 0o log amplifiers
must be adjusted (VP-29) or the MAM must be replaced.:
0o Equation:
7.00 RBC 0o P.C. = 1.40 ± 0.04
4.99 RBC 0o P.C.
16. Plug the appropriate 10o peak channel numbers into the
following equation, and calculate the 10o log amplifier
ratio. If the ratio is out-of-specifications, the 10o log
amplifiers must be adjusted (VP-30) or the MAM must be
replaced.
10o Equation:
7.00 RBC10o P.C. = 1.67 ± 0.04
3.xx PLT10o P.C.
13. When cycle is completed, view “PLT 10 DATA” and “PLT
10 HISTOGRAM”, and verify the peak is in channel (120
+/- 1 for 3.29 or 121+/- 1 for 3.31). If the PLT 10o peak
channel number is within specifications, record the PLT 10
peak channel number, and skip step 14.
14. Perform RBC/PLT 10o Gain Verification/Adjustment
(VP-27), and repeat steps 12 and 13.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
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8#0#9HULILFDWLRQ#3URFHGXUHV • VP-29 0° MAM Log Amplifier Verification/Adjustment
VP-29 0° MAM Log Amplifier
Verification/Adjustment
The following procedure verifies and, if necessary, adjusts the
0o log amplifier gains and offsets on the MAM. Refer to Figure
5-6. A voltage-divider is used to inject a 10mv reference voltage
into the 0o log amplifier, and gains and offsets are adjusted to
provide the initial setup of the 0o log amplifier chain.
Figure 5-6
Voltage-Divider Fixture
R ed (+1 5V )
5K O H M S
100 O H M S
3 2 0 80
Required Tools & Materials:
•
Voltage-Divider Fixture, PN 9450202
•
Digital Voltmeter
•
Potentiometer Adjustment Tool or Small Slotted
Screwdriver
Verification/Adjustment Procedure:
1. Refer to Figure 5-6. Install the fixture on the MAM by
attaching the red lead to the top of L2, black lead to TP7,
and the yellow lead to TP8.
2. Attach the positive lead of the DVM to TP8 and the
negative lead to TP7.
Yellow (+10m v)
3. Adjust the pot on the fixture for a reading of +10.0mv +/0.2mv.
B lack (G N D )
4. Move the positive lead to TP11, and adjust R102 for
-23.0mv +/- 0.2mv
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 32
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-29 0° MAM Log Amplifier Verification/Adjustment
Figure 5-7
MAM Test Points & Potentiometers
TP 2
R84
3 2 0 81
R102
TP 11
R106
TP 9
TP 7
R137
TP 8
TP 1 7
R132
TP 1 5
L2
TP 1 8
R138
TP 2 1
R131
TP 1 6
5. Move the positive lead to TP15, and adjust R132 for
-1.000V +/- 0.010V.
6. Move the positive lead to TP16, and adjust R137 for 3.68V
+/- 0.010V.
7. Leave meter as is, and adjust R132 for a reading of 1.72V
+/- 0.010V.
NOTE: After performing this procedure the 0o gain must
be verified/adjusted with Beads (VP-26).
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 33
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-30 10° MAM Log Amplifier Verification/Adjustment
VP-30 10° MAM Log Amplifier
Verification/Adjustment
The following procedure verifies and, if necessary, adjusts the
10o log amplifier gains and offsets on the MAM. Refer to Figure
5-8. A voltage-divider is used to inject a 10mv reference voltage
into the 0o log amplifier, and gains and offsets are adjusted to
provide the initial setup of the 10o log amplifier chain.
Figure 5-8
Voltage-Divider Fixture
R ed (+1 5V )
5K O H M S
100 O H M S
3 2 0 80
3. Adjust the pot on the fixture for a reading of +10.0mv +/0.2mv.
4. Move the positive lead to TP18, and adjust R106 for
-10.0mv +/- 0.2mv
5. Move the positive lead to TP21, and adjust R131 for
-1.000V +/- 0.010V.
6. Move the positive lead to TP9, and adjust R138 for 4.20V
+/- 0.010V.
7. Leave meter as is, and adjust R131 for a reading of
0.750V +/- 0.010V.
NOTE: After performing this procedure the 10o gain must
be verified/adjusted with Beads (VP-27).
Yellow (+10m v)
B lack (G N D )
Required Tools & Materials:
•
Voltage-Divider Fixture, PN 9450202
•
Digital Voltmeter
•
Potentiometer Adjustment Tool or Small Slotted
Screwdriver
Verification/Adjustment Procedure:
1. Refer to Figure 5-6. Install the fixture on the MAM by
attaching the red lead to the top of L2, black lead to TP7,
and the yellow lead to TP17
2. Attach the positive lead of the DVM to TP17 and the
negative lead to TP7.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
VP-31 Aspirate/Vent Needle Verification
Required Tools & Materials:
•
None
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ TOWER TEST
2. Press
„ NEEDLE DOWN
3. Verify that the needle moves down smoothly without any
stopping or jerking. Also verify that the GS1 sensor status
displays the following:
GS1 UP:SENSOR STATUS
Part Number 9140300 − August 1998
5 - 34
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-32 GS2 Stop Solenoid Verification
GS1=1
VP-33 Bar Code Spin Assembly Verification
GS1 DOWN:SENSOR STATUS
GS1=0
4. Press
„ NEEDLE UP
5. Verify that the needle moves up smoothly without any
stopping or jerking.
VP-32 GS2 Stop Solenoid Verification
Required Tools & Materials:
•
None
Verification/Adjustment Procedure:
1. From Main Menu, press
Required Tools & Materials:
•
None
Verification/Adjustment Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ TOWER TEST
2. Place a tube at the read station, and press
„ SPIN MOTOR
3. Verify that the tube is spun smoothly without any stopping
or jerking.
4. Press
„ DIAGNOSTICS
„ STOP MOTOR
„ MORE
to return to “DIAGNOSTICS.”
„ TOWER TEST
2. Alternately press “Retract Solenoid” and “Extend
Solenoid.”
VP-34 Tube Height Sensors (S1/S2)
Verification
3. Verify that the GS1 solenoid retracts and extends
smoothly and fully with each command. Also verify that
LED DS1 on SHM1 is on with the retract command and off
with the extend command.
This diagnostic routine first moves GS1 and GS2 down and
then up in the same manner as a count cycle. It then displays
the status of sensors GS1, S1, and S2 in both the up and down
positions (1=sensor blocked, 0=sensor unblocked). It also
displays the type of tube sensed at the aspirate position: BD,
SST, or No Tube.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 35
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-35 Mixer Up/Down Verification
Required Tools & Materials:
•
BD Specimen Tube
•
Sarstedt Specimen Tube
Verification/Adjustment Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
GS1=0 S1=1 S2=1SST
No Tube Example:
GS1 UP: Sensor Status
GS1=1 S1=0 S2=0
GS1 Down: Sensor Status Tube Type
GS1=0 S1=0 S2=1 No Tube
„ MORE
„ TOWER TEST
2. Place the appropriate tube at the vent/aspirate position.
3. Press
„ SENSOR TEST
„ GS1 DOWN
4. Verify the following sensor status and tube type for each
tube condition.
VP-35 Mixer Up/Down Verification
Required Tools & Materials:
•
Verification/Adjustment Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
BD Tube Example:
„ MORE
GS1 UP: Sensor Status
GS1=1 S1=0 S2=0
GS1 Down: Sensor Status Tube Type
GS1=0 S1=1 S2=0BD
Sarstedt Tube Example:
None
„ LOADER TEST
2. Alternately press “MIXER DOWN” and “MIXER UP” and
verify that the assembly moves up and down smoothly
without any stopping or jerking. Also verify that the home
sensor is “1” when up and “0” when down.
VP-36 Mixer Head Rotation Verification
GS1 UP: Sensor Status
GS1=1 S1=0 S2=0
GS1 Down: Sensor Status Tube Type
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Required Tools & Materials:
•
None
Part Number 9140300 − August 1998
5 - 36
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-37 Mixer Bladders Verification
Verification/Adjustment Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ LOADER TEST
2. Alternately press “ROTATE MIX UP” and “ROTATE MIX
DOWN” and verify that the mixer head rotates up and
down smoothly without any stopping or jerking. Also verify
that the home sensor is “0” when up and “1” when down.
VP-37 Mixer Bladders Verification
Required Tools & Materials:
•
None
Verification/Adjustment Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ LOADER TEST
2. Press
„ CYCLE BLADDERS
VP-38 Rack Advance & Tube Sensors
Verification
Required Tools & Materials:
•
Specimen Tubes
•
Specimen Loader Rack
Verification/Adjustment Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ LOADER TEST
2. Place a rack with alternating tubes at the load side rear
wall.
3. Press
„ START RACK ADVANCE
4. Verify that the rack indexes smoothly through the
processing station without any stopping or jerking during
index movements.
5. Verify that when a tube is present at a sensor position a “1”
is indicated, and when a tube is not present a “0” is
indicated.
3. Alternately press “GRIP TUBE” and “RELEASE TUBE”
and verify that the mixer bladders inflate and deflate
completely, and that there are no pressure or vacuum
leaks.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 37
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-39 Cross Transfer Arms & Rack Sensors Verification
VP-39 Cross Transfer Arms & Rack Sensors
Verification
5. Remove rack from the load side, place five racks in the
unload side, and press
„ EXTEND ARMS
Verify the following sensor status, and press “RETRACT
ARMS.”
Required Tools & Materials:
•
5 Racks
SENSOR STATUS
Verification/Adjustment Procedure:
EXTEND ARMS:LOAD SENSOR = 1
1. From Main Menu, press
UNLOAD FULL
„ DIAGNOSTICS
UNLOAD WARN = 1
„ MORE
„ LOADER TEST
2. Leave the load side and unload side empty, and
alternately press “EXTEND ARMS” and “RETRACT
ARMS.”
3. Verify that the arms extend and retract smoothly without
any stopping or jerking.
4. Place one rack in the load side, leave unload side empty,
and press
„ EXTEND ARMS
Verify the following sensor status, and press
„ RETRACT ARMS
SENSOR STATUS
EXTEND ARMS:LOAD SENSOR = 0
UNLOAD FULL
=0
=1
VP-40 Rack Bar Code Labels Verification
Required Tools & Materials:
•
5 Racks
Verification/Adjustment Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ VER RACK BAR CODE
2. Manually move a rack through the processing station,
positioning the rack and tube position bar code labels at
the slot in the processing station.
3. Verify that the bar code number on the screen matches the
number on the rack.
UNLOAD WARN = 0
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 38
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-41 Mixer Bladders Pressure Verification/Adjustment
VP-41 Mixer Bladders Pressure
Verification/Adjustment
7. Adjust pressure regulator (just to right of load side empty
sensor) for 6.5 psi +/- 0.2 psi.
8. Reconnect tubing to vacuum/pressure port.
Required Tools & Materials:
•
Pressure Gauge
•
Slotted Screwdriver
Verification/Adjustment Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ VOLTAGE READINGS
2. Verify “Press 1 psi” is 12.5-13.5 psi. If out-of-range,
perform Vacuum & Pressure Level Verification/Adjustment
(VP-16).
3. Press
„ MORE
VP-42 Shear Valve Driver Lubrication
Procedure
Required Tools & Materials:
•
Oil PN 14237-015 (Dallas/Europe), 1605806 (Santa Clara)
Verification Procedure:
1. Power off instrument using the proper shutdown
procedure.
2. Remove the shear valve driver from the Analyzer.
3. Lubricate the assembly as shown in Figure 5-9.
4. Re-install the shear valve driver and verify instrument
performance.
„ LOADER TEST
4. Press
„ CYCLE BLADDERS
5. Remove tubing from vacuum/pressure port on mixer head,
and connect tubing to pressure gauge.
6. Press
„ GRIP TUBE
to apply pressure.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 39
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-43 Optics Bench Cleaning Procedure
Figure 5-9
Shear Valve Driver Lubrication Points
2-3 D rop s O f O il 3 P lace s
F ront
3 2 0 82
2-3 D rop s O f O il 2 P lace s
VP-43 Optics Bench Cleaning Procedure
'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.
Required Tools & Materials:
•
Cotton Swabs
•
High Grade Lens Paper (Kodak recommended - DO NOT
USE Baxter S/P, as it may scratch the lens.)
•
Canned Air - Optics Grade
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Verification Procedure:
1. Clean the left side and rear fan filters.
2. Remove Analyzer top cover and plastic optics covers.
3. Close Laser Shutter.
4. Using pressurized air, remove dust and dirt from optics
bench and surrounding area.
Part Number 9140300 − August 1998
5 - 40
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-43 Optics Bench Cleaning Procedure
5. Using Kodak lens paper on the end of a cotton swab,
clean rear mirror, cylindrical lens, front mirror, imaging
lens, and flow cell.
&$87,21=##Do not use lens cleaning solution on
mirrors. If cleaning fluid is needed, use reagent grade
methanol only.
9. Check for a red glow or halo around the forward slit. If
laser output lens dirty, a halo will appear around football
shaped laser beam. Refer to Figure 5-11.
Figure 5-11
Forward Slit Halo
F orw ard
S lit
F ootball
S haped
B eam
6. Repeat cleaning process to ensure all residual particles
are removed.
7. Open Laser Shutter.
8. With laser on, check for a red glow or halo around opening
where laser beam exits the laser tube. Refer to Figure
5-10.
Figure 5-10
Laser Output Halo
N O R ed
H alo
3 2 0 84
Laser Tube
R ear
M irror
N O R ed
H alo
3 2 0 83
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 41
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-43 Optics Bench Cleaning Procedure
10. If halo exists in either location, clean laser with dry cotton
swab, or use lens paper. Refer to Figure 5-12.
Figure 5-12
Figure 5-13
Mark Mirror Positions
M ark
Location
Laser Tube Cleaning
Laser Tube
R ear
M irror
3 2 0 87
Insert C otton
S w ab H ere
b. Remove screws and mirror. Refer to Figure 5-14.
3 2 0 85
Figure 5-14
Mirror Removal
11. Close Laser Shutter.
Tape
12. Clean 10o mirror.
a. Mark position of mirror and housing with pencil. Refer
to Figure 5-13.
R em ove
M irror
3 2 0 88
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 42
5 - Verification Procedures • VP-44 Optical Flow Cell Cleaning & De-Bubble Procedure
c. Clean mirror using reagent grade methanol or
microscope lens cleaning solution (green) and high
grade lens paper. (Kodak recommended - DO NOT
USE Baxter S/P). Ensure surface is clean; use
compressed air to remove any remaining lint.
d. Replace mirror and align marks. DO NOT tighten
screws.
e. Open Laser Shutter.
16. Perform RBC/PLT 0o Gain Verification/Adjustment
(VP-26).
17. Perform RBC/PLT 10o Gain Verification/Adjustment
(VP-27).
VP-44 Optical Flow Cell Cleaning & De-Bubble
Procedure
DANGER: LASER LIGHT Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.
13. Use the following procedure to adjust the mirror for
maximum 10o mean.
a. Prepare the WBC Beads solution by placing 2 mL of
diluent in a clean container, adding 15 drops of 7.00 µ
beads, and mixing well.
b. From Main Menu, press
n
n
n
DIAGNOSTICS
MORE
WBC DATA
c. Place the 7.00 µ beads solution under the probe, and
press
n
n
n
EXTENDED WBC
COUNT
START
to aspirate the Beads and start the cycle.
d. Observe the 10o mean, and rotate the mirror for the
highest 10o mean reading.
e. Tighten mirror screws ensuring maximum reading is
maintained, and erase pencil marks.
Required Tools & Materials:
•
20% Bleach or Abbott, ISE Conditioning Solution
LN1360-02
•
10 mL Syringe
•
Needle Nosed Pliers
Verification Procedure:
1. Fill syringe with 20% bleach solution or ISE Cleaning
Solution.
2. From Main Menu, press
n SPECIAL PROTOCOLS
n EMPTY/FILL FLOW CELL
n EMPTY FLOW CELL
14. Perform WBC Gain Verification/Adjustment (VP-23).
15. Perform NOC Gain Adjustment (VP-25).
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 43
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-44 Optical Flow Cell Cleaning & De-Bubble Procedure
3. From Main Menu, press
Figure 5-15
Flow Cell Cleaning Plumbing
„ DIAGNOSTICS
„ MORE
„ SOLENOID OPERATION
Flo w C ell D e-Bu b b le
P ro ce s s
4. To open solenoid 56, press
„ STEP SOLENOID
type 56, and press
„ ENTER
5. Refer to Figure 5-15. Remove elbow from right side of
solenoid 57, and connect elbow to syringe.
6. Inject bleach or cleaning solution in short strokes to create
turbulent motion in flow cell.
7. Let solution remain is flow cell for three to five minutes.
&$87,21=##Do not allow ISE cleaning solution to remain in
flow cell for over five minutes.
56
57
In je c t 20 %
B le a ch o r
IS E C le an in g
S o lu tio n
& Air a t
Th is P o in t
91
3 2 0 89
8. Disconnect syringe, and connect reconnect elbow to
solenoid 57.
9. Initialize and prime instrument, and run three background
counts.
10. Verify that background counts are within specifications:
WBC ≤ 0.10 K/µL, RBC ≤ 0.02 M/µL, and PLT ≤ 5 K/µL.
11. Refer to Figure 5-15. Verify that there are no large bubbles
in the flow cell. If large bubbles are present, perform
Optical Flow Cell Wetting Procedure (VP-43).
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 44
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-45 Optical Flow Cell Wetting Procedure
VP-45 Optical Flow Cell Wetting Procedure
'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.
Required Tools & Materials:
•
CN Free HGB/NOC Lyse
•
10 mL Syringe
6. Inject HGB lyse reagent in short strokes to create turbulent
motion in flow cell.
7. Let lyse remain is flow cell for three to five minutes.
8. Disconnect syringe, and connect reconnect elbow to
solenoid 57.
9. Initialize and prime instrument, and run three background
counts.
10. Verify that background counts are within specifications:
WBC ≤ 0.10 K/µL, RBC ≤ 0.02 M/µL, and PLT ≤ 5 K/µL.
1. Fill syringe with HGB lyse solution.
11. Refer to Figure 5-16. Verify that there are no large bubbles
in the flow cell. If large bubbles are present, repeat this
procedure.
2. From Main Menu, press
Figure 5-16
Verification Procedure:
Flow Cell Bubbles
„ SPECIAL PROTOCOLS
„ EMPTY/FILL FLOW CELL
„ EMPTY FLOW CELL
3. From Main Menu, press
„ DIAGNOSTICS
To W a ste /
Va cu u m
C e ll
S tre a m
N O L a rg e
A ir B u b b le s
Fe w S m a ll A ir
B ub b le s a re O K
„ MORE
„ SOLENOID OPERATION
4. To open solenoid 56, press
„ STEP SOLENOID
type 56, and press
„ ENTER
5. Refer to Figure 5-15. Remove elbow from right side of
solenoid 57, and connect elbow to syringe.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
S he a th P rim e
S he a th In
3 2 0 90
S am p le In
Part Number 9140300 − August 1998
5 - 45
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-46 Laser Power Output Verification
VP-46 Laser Power Output Verification
'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.
Figure 5-17
Laser Output Power Check
Power
M e te r
5 MW
M o u n t C e n te re d
in B e a m Pa th
Required Tools & Materials:
•
Laser Power Meter
•
Optical Alignment Tool Kit, PN 8921183801
32091
Verification Procedure:
1. Ensure laser has been on for at least 15 minutes.
2. Perform Optics Bench Cleaning Procedure (VP-43).
3. Refer to Figure 5-17. Check laser power after first mirror:
a. Mount power meter photodiode centered in beam path.
b. Verify that power is 5 mW or greater.
c. If power is less than 5 mW, replace laser tube.
4. Refer to Figure 5-18. Check laser power after forward slit:
a. Mount power meter photodiode centered in beam path
between forward slit and imaging lens.
b. Power meter should read at least 1/5 of that obtained
in step 3.
5. Perform Optical Offsets Verification (VP-47).
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 46
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-47 Optical Offsets Verification
Figure 5-18
Laser Power At Forward Slit
3. Verify that CH 1 through CH4 Offsets are less than 1.00
volt.
4. If any are greater than 1.00 volts, perform Optics Bench
Cleaning Procedure (VP-43).
VP-48 Shear Valve Cleaning Procedure
Required Tools & Materials:
P ow e r
M e ter
1 /5 o f P o w e r
o f First M irro r
•
DI Water
•
Clean Container
•
Lint Free Wipes
3 2 0 92
Verification Procedure:
1. From Main Menu, press
„ SPECIAL PROTOCOLS
VP-47 Optical Offsets Verification
Required Tools & Materials:
•
Optical Alignment Tool Kit, PN 8921183801
„ CLEAN/RES SHEAR VAL
„ CLEAN SHEAR VAL
2. Refer to Figure 5-19. Unscrew shear valve knob.
&$87,21=##Do not drop center ceramic section.
Verification Procedure:
1. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ VOLTAGE READINGS
2. Ensure top cover is in place, or cover optics bench with
black cloth.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
3. Remove three ceramic sections from shear valve driver
leaving tubing in place, and place center ceramic piece in
a container of DI water.
4. Use damp lint free wipes to clean front and rear surfaces
of front and rear ceramic sections.
Part Number 9140300 − August 1998
5 - 47
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-49 HGB Flow Cell Cleaning Procedure
5. Clean center mounting holes in front and rear ceramic
sections.
Figure 5-19
Shear Valve Disassembly
VP-49 HGB Flow Cell Cleaning Procedure
This procedure first uses the Auto Clean routine to clean the
flow cell, and if that is unsuccessful a manual procedure is
used.
Loop
Rear
S he a r
Va lv e
B lo ck
C e n te r
Fron t
Loop
M o un tin g G u id e
K no b
R im N o tch
L o ck N otc h
Required Tools & Materials:
•
CELL-DYN Enzymatic Cleaner
•
20% Bleach Solution
•
20 mL Syringe
3 2 0 93
Verification Procedure:
1. From Main Menu, press
6. Clean mounting guide and lock notches in each ceramic
section.
„ SPECIAL PROTOCOLS
7. Using a flashlight, inspect ports on front and rear ceramic
sections, and ensure no foreign material blocks ports.
„ AUTO CLEAN
8. Remove center section from DI water, and clean both
sides to remove any blood or foreign material.
9. Wet all surfaces with DI water and assemble as shown in
Figure 5-19. Ensure wide notches are down and rim notch
on center section is to the right.
„ MORE
Follow the on-screen instructions to perform the
procedure.
2. Run three background counts to purge enzyme cleaner.
3. From Main Menu, press
„ DIAGNOSTICS
10. Tighten shear valve knob until it stops.
„ MORE
11. Press
„ VOLTAGE READINGS
„ RESTORE SHEAR VAL
12. Run background counts until all are within specifications:
WBC ≤ 0.10 K/µL, RBC ≤ 0.02 M/µL, and PLT ≤ 5 K/µL.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
4. Verify that the “HGB OUTPUT” voltage is 5.00 volts
+0.2/-0.00 volts. If the voltage is within range, skip steps 5
through 13.
5. Manually open solenoid 93 to drain liquid from flow cell.
Part Number 9140300 − August 1998
5 - 48
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-50 Open Mode Aspiration Path Cleaning Procedure
6. Prepare and aspirate 20% bleach solution into syringe.
7. Refer to Figure 5-20. Remove tubing from port and
connect syringe to port.
8. Inject bleach solution until it can be seen exiting top port,
and let remain in flow cell for five to ten minutes.
13. Verify that the “HGB OUTPUT” voltage is 5.00 volts
+0.2/-0.00 volts. If voltage is slightly out-of-range, perform
HGB Current Verification/Adjustment (VP-5).
VP-50 Open Mode Aspiration Path Cleaning
Procedure
9. Manually open solenoid 93 to drain bleach from flow cell.
:$51,1*=#327(17,$/#%,2+$=$5' The
aspiration needle/probe are sharp and potentially
contaminated with infectious materials. Avoid
contact with the tips of the needle/probe.
10. Remove syringe, and connect tubing to port.
11. Run three background counts to purge bleach.
Figure 5-20
HGB Flow Cell Plumbing
Required Tools & Materials:
Inje ct B lea ch
To Here
R em ove Tu bing
& Co nnect Syringe
H GB Flow Ce ll
•
CELL-DYN Enzymatic Cleaner
•
Specimen Tubes
•
Wire Stylet, Gauge #23
•
Lint Free Tissues
•
Diluent
Verification Procedure:
3 2 0 94
12. From Main Menu, press
1. In the “RUN” mode use “CHANGE SAMPLER” to select
“OPEN SAMPLER.”
2. Manually open solenoid 12 to purge liquid from plumbing.
„ DIAGNOSTICS
3. Remove tubing from top of open probe.
„ MORE
4. Place a tissue under probe, run stylet into the top of probe
until it extends out the bottom, and wipe any material from
stylet. Run up and down several times and remove.
„ VOLTAGE READINGS
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 49
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-51 Closed Mode Aspiration Path Cleaning Procedure
5. Clean outside of probe several times with tissue
dampened with Enzymatic Cleaner, and reconnect tubing
to top.
6. Place a tube containing 4 mL of Enzymatic Cleaner under
probe, manually open solenoid 12, and close when
Enzymatic Cleaner passes through solenoid 12.
7. Let Enzymatic Cleaner remain for five minutes, and
manually open 12 until plumbing is emptied.
8. Place a tube filled with diluent under probe, and open 12
until tube is emptied to flush plumbing.
9. Run background counts until all are within specifications:
WBC ≤ 0.10 K/µ L, RBC ≤ 0.02 M/µ L, and PLT ≤ 5 K/µL.
VP-51 Closed Mode Aspiration Path Cleaning
Procedure
3. Manually open solenoid 12 to purge liquid from plumbing.
4. Place a tube filled with enzyme cleaner at the aspirate
position, and press
„ NEEDLE DOWN
5. Manually open solenoid 12, and close when enzyme
cleaner passes through solenoid 12.
6. Manually hold solenoid 38 closed, and momentarily open
solenoid 36 to aspirate enzyme cleaner into vent needle.
7. Let enzyme cleaner remain for five minutes, press
„ NEEDLE UP
and manually open 12 until plumbing is emptied.
8. “Initialize” and “Prime” instrument.
9. Run background counts until all are within specifications:
WBC ≤ 0.10 K/µL, RBC ≤ 0.02 M/µL, and PLT ≤ 5 K/µL.
Required Tools & Materials:
•
CELL-DYN Enzymatic Cleaner
•
Specimen Tubes
Verification Procedure:
1. In the “RUN” mode use “CHANGE SAMPLER” to select
“CLOSED SAMPLER.”
2. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ TOWER TEST
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 50
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-52 Power-Up CPU/DCM 7-Segment LED Verification
VP-52 Power-Up CPU/DCM 7-Segment LED
Verification
VP-53 Software Installation Procedure
Required Tools & Materials:
Required Tools & Materials:
•
•
CELL-DYN System Installation Disks 1 & 2 P.N. 9380590
None
Verification Procedure:
Verification Procedure:
1. Power-up the system and observe the 7-segment LED on
the CPU/DCM. Refer to the following table for status
conditions.
NOTE: After all the tests pass and solenoids energize, “1”
and “E” will alternately flash in the display. This is a normal
condition and does not indicate a problem.
Table 5-6
CPU/DCM 7-Segment LED Status Conditions
1. Turn system power off.
2. Insert disk #1 in the floppy drive.
3. Turn system power on.
4. Follow the on-screen instructions to install software.
5. At the C:\CD3200> prompt type A:INSTALL and press
„ ENTER
6. When prompted, install disk #2 and follow the on-screen
directions.
“0”
Indicates system power-up.
“1”
Indicates that the CPU/DCM flash memory failed.
“2”
Indicates that the CPU/DCM first 524K of DRAM
failed.
“3”
Indicates that the CPU/DCM second 524K of
DRAM failed.
VP-54 Hard Drive Verification
“4”
Indicates that the CPU/DCM third 524K of DRAM
failed.
Required Tools & Materials:
“5”
Indicates that the CPU/DCM fourth 524K of DRAM
failed.
“6”
Indicates that all CPU/DCM memory passed.
7. When installation is complete, remove disk #2 and
power-down and re-boot instrument.
•
None
Verification Procedure:
1. In the “MAIN” menu press
„ F12
„ F8
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 51
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-55 Floppy Drive Verification
2. At the C:\CD3200 prompt, type CHKDSK/F and press
VP-55 Floppy Drive Verification
„ ENTER
Required Tools & Materials:
3. At the selection prompt type Y and press
•
„ ENTER
to run CHKDSK/F.
.
Verification Procedure:
1. In the “MAIN” menu, press
1 ,2 7 9 ,6 8 8 ,7 0 4 b y te s to ta l d is k sp a c e
1 3 1,0 7 2 b y te s in 2 h id d e n file s
„ F12
3 ,1 7 8 ,4 9 6 b yte s in 9 7 d ire cto rie s
7 5 0 ,22 3 ,3 6 0 b y te s in 2 ,2 1 5 u s e r file s
5 2 6 ,1 5 5,7 7 6 b y te s a v a ila b le o n d is k
„ F8
2. At the C:\CD3200 prompt, type A: and press
3 2 , 7 6 8 b y te s in e a c h a llo c a tio n u n it
3 8 ,0 53 to ta l a llo ca tio n u n its o n d is k
16 ,0 5 7 a v a ila b le a llo ca tio n u n its o n d is k
6 5 5 ,3 6 0 to ta l b yte s m e m ory
6 1 8 ,5 6 0 b yte s fre e
Figure 5-21
None
„ ENTER
3. At the A:\> prompt, type CHKDSK/F and press
„ ENTER
4. At the selection prompt type Y and press
3 2 0 95
CHKDSK Results, Hard Disk
4. If lost allocation units are reported, type N to delete.
5. The display should closely resemble Figure 5-22. If a line
is added saying “xxxx bytes in bad sectors” verify that
the number of bytes is less than 1% of the total disk space.
If the number exceeds 1%, the hard drive is suspect and
should probably be replaced.
„ ENTER
to run CHKDSK/F.The display should closely resemble
Figure 5-22.
5. If there are no indications of a defect, type C: and press
„ ENTER
6. Then type rll and press
„ ENTER
to return to the “MAIN” menu.
6. If there are no indications of a defect, type rll and press
“ENTER” to return to the “MAIN” menu.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 52
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-56 Background Counts Verification
Table 5-7
1 ,4 5 7 ,6 6 4 bytes total disk space
1 9 5 ,0 7 2 bytes in 4 user files
1 ,2 6 2 ,5 9 2 bytes available on disk
5 1 2 bytes in each allocation unit
2 ,8 4 7 total allocation units on disk
2 ,4 6 6 available allocation units on disk
6 5 5 ,3 6 0 total bytes memory
6 1 8 ,5 6 0 bytes free
Figure 5-22
Floppy Drive CHKDSK Results
Background Count Specification
WBC
(WOC)
NOC
RBC
HGB
≤ 0.10 k/µ L ≤ 0.10 k/µL ≤ 0.02 m/µL ≤ 0.1g/dL
PLT
≤ 5.0 k/µL
VP-57 System CV Verification
Required Tools & Materials:
•
2.5 mL Fresh Normal Blood
Verification Procedure:
1. From Main Menu, press
VP-56 Background Counts Verification
Required Tools & Materials:
•
None
Verification Procedure:
1. From Main Menu, press
„ RUN
„ SPECIMEN TYPE
„ BACKGROUND
2. Press the “TOUCH PLATE” to run a background count.
3. Verify that the background counts are within specifications
according to the following table.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
„ RUN
„ SPECIMEN TYPE
2. Move the cursor to an empty file, and press
„ QC SPECIMEN
3. Mix the fresh blood thoroughly, and run 10 times in the
open mode and 10 times in the closed mode.
4. Press
„ MAIN
„ QUALITY CONTROL
„ VIEW QC LOG
5. Verify the CVs are within specification according to the
following table.
Part Number 9140300 − August 1998
5 - 53
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-58 System Calibration Verification/Adjustment
Table 5-8
b. From MAIN MENU press
CV Specifications
WBC
NOC
(WOC)
RBC
HGB
MCV
PLT
„
„
MPV
and toggle “F2” to “CALIBRATOR.”
≤ 2.7% ≤ 2.7% ≤ 1.5% ≤ 1.0% ≤ 1.0% ≤ 4.0% ≤ 5.0%
c. Enter the reference values from the assay sheet,
pressing [ENTER] after each entry.
VP-58 System Calibration
Verification/Adjustment
d. Press
„
e. Prepare the calibrator for processing according to the
assay sheet.
CELL-DYN Calibrator
f.
Verification Procedure:
„
a. Verify reagent type, expiration date, and volumes are
at least 1/2 full.
e. Verify system precision is within specifications (VP-57).
2. Perform Open Mode Auto Calibration using commercial
calibrator only.
NOTE: If any problems are encountered, refer to
“Auto-Cal Procedure-Open Mode” in Chapter 6 of the
CELL-DYN 3200 Operations Manual for a complete
description of the calibration procedure.
ACCEPT
to enter the new factors for the parameters that need
calibration.
b. Verify calibrators are not expired.
d. Ensure WOC offsets for channels 1 through 4 are less
than 1.00 volt.
Run the calibrator 5 times, mixing well between runs.
g. Press
1. Pre-calibration checks:
c. Ensure background counts are within specifications
(VP-56).
START AUTO-CAL
to ready the instrument for calibration.
Required Tools & Materials:
•
CALIBRATION
AUTO-CALIBRATE
VP-59 Vacuum Accumulator #1 Rinsing
Procedure
Required Tools & Materials:
•
Clean >250 mL Container
•
DI Water
•
0.125"ID 0.250"OD Tubing, 1 Foot
•
#2 Phillips Screwdriver
a. Ensure Analyzer in Open Mode.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 54
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-60 Vacuum Accumulator #2 Rinsing Procedure
Verification Procedure
1. Initialize Analyzer, and remove Left Side Cover.
5. Place tubing connected to position 4 into container, and
allow all DI water to be aspirated into Vacuum
Accumulator #1.
2. Extend Air Supply out of Analyzer (Vacuum/Pressure
Assembly (RR-7)).
6. Remove tubing from position 4, connect original tubing,
and unpinch position 7.
3. Measure 250 mL (1 cup) of DI water into a clean container.
7. Wait at least 5 minutes to remove any saline bridges
between electrodes.
&$87,21=##Do not place more than stated amount of
DI water in container.
8. From Main Menu, press
„ DIAGNOSTICS
TO
FL O W PA N E L
9P SI
5P SI 12"H g 8"H g 12P SI 12"H g
„ DRAIN ACCUMULATORS
9. Initialize Analyzer and repeat Step 8.
Required Tools & Materials:
32004
Figure 5-23
„ MORE
VP-60 Vacuum Accumulator #2 Rinsing
Procedure
12P SI
1
2
3
4
5
To S L
6
To S L
7
4. Refer to Figure 5-23. Pinch tubing at position 7 of
manifold, remove tubing from position 4, and attach
0.125"ID 0.250"OD tubing to fitting at position 4.
Vacuum/Pressure Manifold
CELL-DYN 3200 SYSTEM SERVICE MANUAL
•
Clean >250 mL Container
•
DI Water
•
0.125"ID 0.250"OD Tubing, 1 Foot
•
#2 Phillips Screwdriver
Verification Procedure
1. Initialize Analyzer, and remove Left Side Cover.
2. Extend Air Supply out of Analyzer (Vacuum/Pressure
Assembly (RR-7)).
Part Number 9140300 − August 1998
5 - 55
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-61 Hard Drive Formatting
1
6. Remove tubing from position 5, and connect original
tubing.
7. Wait at least 5 minutes to remove any saline bridges
between electrodes.
8. From Main Menu, press
5P SI 12"H g 8"H g 12P SI 12"H g
TO
FL O W PA N E L
2
5. Place tubing connected to position 5 into container, and
allow all DI water to be aspirated into Vacuum
Accumulator #2.
9P SI
3
4. Refer to Figure 5-24. Remove tubing from position 5 of
manifold, and attach 0.125"ID 0.250"OD tubing to fitting at
position 5.
12P SI
4
5
To S L
6
To S L
&$87,21=##Do not place more than stated amount of
DI water in container.
7
3. Measure 250 mL (1 cup) of DI water into a clean container.
32004
Figure 5-24
Vacuum/Pressure Manifold
VP-61 Hard Drive Formatting
„ DIAGNOSTICS
Required Tools & Materials:
„ MORE
CELL-DYN 3200 System Service Disk PN 9380700
„ IDRAIN ACCUMULATORS
9. Initialize Analyzer and repeat Step 8.
Verification Procedure
1. Perform (VP-64) “Backup Procedure” .
2. Turn System OFF.
3. Insert Service Disk into Floppy Drive.
4. Turn System ON.
5. At A:\ prompt type CHKDSK/F, then press
„ ENTER
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 56
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-62 DOS Installation
6. If there is any indication that the disk is defective, it should
be formatted.
7. Format the hard drive:
5. Remove Service Disk.
ENTER
b. The display will indicate when the format is complete
8. Perform (VP-65) Restore Setup
1 ,2 7 9 ,6 8 8 ,7 0 4 b y te s to ta l d is k sp a c e
1 3 1,0 7 2 b y te s in 2 h id d e n file s
Required Tools & Materials:
3 ,1 7 8 ,4 9 6 b yte s in 9 7 d ire cto rie s
7 5 0 ,22 3 ,3 6 0 b y te s in 2 ,2 1 5 u s e r file s
5 2 6 ,1 5 5,7 7 6 b y te s a v a ila b le o n d is k
None
Verification Procedure
3 2 , 7 6 8 b y te s in e a c h a llo c a tio n u n it
3 8 ,0 53 to ta l a llo ca tio n u n its o n d is k
16 ,0 5 7 a v a ila b le a llo ca tio n u n its o n d is k
Figure 5-25
CHKDSK/F Results, Hard Disk
6. Turn System OFF, wait 5 seconds, then turn System ON.
VP-63 CS/SL SETUP
.
6 5 5 ,3 6 0 to ta l b yte s m e m ory
6 1 8 ,5 6 0 b yte s fre e
4. At A:\ prompt type INSTALL, then press
„ ENTER
a. Type FORMAT C:/S and press
„
3. Turn System ON.
3 2 0 95
1. Enter Diagnostics Hidden Screen
a. From MAIN MENU, press
„
DIAGNOSTICS
b. Press
„
„
F12
F1
TOGGLE CS/SL to appropriate instrument type.
VP-62 DOS Installation
Required Tools & Materials:
Service Disk PN 9380700
NOTE: Instrument type will display on screen.
c. Press
„
RETURN
Verification Procedure
1. Turn System OFF.
2. Insert Service Disk into Floppy Drive.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 57
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-64 Backup Procedure
VP-64 Backup Procedure
4. At A:\ prompt, type R to RESTORE, then press
„ ENTER
Required Tools & Materials:
CELL-DYN 3200 System Setup Disk PN 9380595
This copies Old Version Setup from Setup Disk to Hard
Drive.
5. Remove Setup Disk.
Verification Procedure
6. Turn System OFF, wait 5 seconds, then turn System ON.
1. Turn System OFF.
2. Insert Setup Disk.
3. Turn System ON.
4. At A:\ prompt, type S to SAVE, then press
„ ENTER.
This copies Old Version Setup from Hard Drive to Setup
Disk.
5. Remove Setup Disk.
6. Turn System OFF, wait 5 seconds, then turn System ON.
VP-65 Restore Setup
Required Tools & Materials:
CELL-DYN 3200 System Setup Disk PN 9380595
Verification Procedure
1. Turn System OFF.
2. Insert Setup Disk.
VP-66 List Mode Data Collection
Required Tools & Materials:
None
Verification Procedure
1. Collect Data with List Mode ON.
a. From MAIN MENU, Press
„
DIAGNOSTICS
b. Press,
„
„
„
F12
F1
STORE RAW ON/OFF (To ON)
c. Press,
„
RETURN
d. From MAIN Menu, press
„
RUN
e. Run specimens to collect list mode data.
3. Turn System ON.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 58
8#0#9HULILFDWLRQ#3URFHGXUHV • VP-67 Download HSSL Com
f.
Press
„
„
MAIN
DIAGNOSTICS
g. Press,
„
„
F12
F1
h. DO NOT run analyzer with List Mode ON for an
extended period. Each file = approximately 100kb.
When Hard Drive is filled with Data Lists Fatal Fault
condition will result. Then files must be deleted.
2. Download List Mode Data to Diskette.
a. From MAIN MENU, press
„
„
F12
F8
b. Type CD\RAWFILE\(yymmdd-the DATE OF
COLLECTION).
c. Press
„
ENTER
NOTE: Date format is YYMMDD (Year Month Day).
Example: 960321 = 21 March 1996.
d. Place Formatted 1.44mb diskette in Floppy Disk Drive.
e. Type
COPY C:\RAWFILE\960321\(SEQUENCE#).RAW B:.
This will copy one file of LIST MODE DATA from Data
Station to Diskette in Floppy Drive.
NOTE: Sequence # = Specimen Sequence number in
Data Log.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Example: “COPY C:\RAWFILE\960921\8411.RAW B:”.
If multiple files need to be downloaded, use wild cards.
Such as sequence # 841?.raw. This will download
8410, 8411, 8412, 8413, etc.
3. If Needed - Delete Data Files from Hard Drive.
a. At C:\ prompt type CD\RAWFILE, and press
„
ENTER
b. Type DIR, and Press
„
ENTER
to get listing of all files in RAWFILE directory.
c. Select files to be deleted.
d. Type DEL C:\RAWFILE\(FILE NAME) for each file to
be deleted.
4. Press
„ RETURN
VP-67 Download HSSL Com
Required Tools & Materials
CELL-DYN 3200 System Installation Disks 1 & 2 P.N. 9380590
Verification Procedure
1. Turn system power OFF.
2. Insert disk #1 in the floppy drive.
3. Turn system power ON.
4. When instructed, insert disk #2.
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5. Follow on-screen instructions to download HSSL Com and
setup CS or SL.
a. Go to the Main Menu and press F12, F8 to get to the
C:\CD3200> prompt.
b. Type HSSL
c. If analyzer is an SL, type 0KL <enter>. SKL should be
displayed in the left column. Type 0K <enter>. 0K
should be displayed in the right column.
d. If analyzer is a CS, type 0KS <enter>. 0KS should be
displayed in the left column. Type 0K <enter>. 0K
should be displayed in the right column.
NOTE: 0 = zero
a. Press F8 to exit to the C:\CD3200> prompt.
b. Turn off analyzer, wait 5 sec, turn on analyzer to
reboot.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Part Number 9140300 − August 1998
5 - 60
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