UNICO SQ UV-2800, SQ UV-2802, SQ UV-2802S, SQ UV-2802PC, SQ UV-2802PCS, SQ UV-3802, SQ UV-4802 Spectrophotometer Service Manual

UNICO SQ UV-2800, SQ UV-2802, SQ UV-2802S, SQ UV-2802PC, SQ UV-2802PCS, SQ UV-3802, SQ UV-4802 Spectrophotometer Service Manual

The UNICO SQ series Spectrophotometer is a reliable and versatile instrument for measuring the absorbance and transmittance of various samples. This series includes models with single beam, split beam, and double beam configurations. The instruments have a wide wavelength range, high spectral dispersion, and excellent performance and stability. The SQ series may be suitable for various tasks such as measuring the absorbance of DNA, RNA, and proteins, as well as for chemical analysis.

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UNICO SQ Series Spectrophotometer Service Manual | Manualzz

UNICO SQ Series

MODEL

UV-2800 / UV-2802 /UV-2802PC/UV-2802S/

UV-2802PCS / UV-3802 / UV-4802

SPECTROPHOTOMETER

SERVICE MANUAL

UNICO

182 Ridge Road, Suite E Dayton, NJ 08810 USA

732-274-1155 Fax: 732-274-1151 WEB:

www.unicosci.com

E-mail: [email protected]

C O N T E N T S

1. Introduction

…………………………………………………… 1

2. Specifications

………………………………………………… 3

3. Layout

…………………………………………………………

4

3.1 Layout of UV-2800

……………………………………………………

4

3.2 Layout of UV-2802(S),UV-2802PC(S)

………………………………

9

3.3 Layout of UV-3802

……………………………………………………

15

3.4 Layout of UV-4802

……………………………………………………

20

4. Optical System

…………………………………………………

25

4.1 Incidence of Light

……………………………………………………… 25

4.2 Dispersion of Light

……………………………………………………

25

4.3 Exit of Light

……………………………………………………………

25

4.3.1 Exit of the Single Beam(UV-2800, UV-2802, UV-2802S)

…………………… 25

4.3.2 Exit of the Split Beam(UV-3802)

………………………………………………

25

4.3.3 Exit of the Double Beam(UV-4802)

…………………………………………… 25

5. Electronic System

……………………………………………

29

5.1

Power Supply System

…………………………………………………

34

5.1.1 Power Supply PCB

……………………………………………………………

36

5.1.2 Power Distributing PCB

………………………………………………………… 37

5.2

Control and Drive System

……………………………………………

38

5.2.1 Main CPU PCB

…………………………………………………………………

41

5.2.2 Drive PCB

………………………………………………………………………

44

5.2.3 Transferring PCB

………………………………………………………………

5.3.1 Amplifier PCB

…………………………………………………………………

46

5.3

Sampling and Signal Processing System

……………………………

48

48

5.3.2 Slave Amplifier PCB

……………………………………………………………

51

5.3.3 Slave CPU PCB

………………………………………………………………

53

6. Trouble Shooting

………………………………………………

56

6.1 Tungsten lamp off

……………………………………………………… 56

6.2 Deuterium lamp off

…………………………………………………… 57

6.3 Memory check failed

…………………………………………………

58

6.4 Printer check failed

…………………………………………………… 58

6.5 Filter positioning failed

………………………………………………… 59

6.6 Light source positioning failed

………………………………………

60

6.7 Auto-cell positioning failed

…………………………………………… 61

6.8 Slit positioning failed

…………………………………………………

62

6.9 Search 656.1nm failed

………………………………………………

63

6.10 “Please restart” warning

……………………………………………

64

6.11 “Battery low” warning

………………………………………………… 64

6.12 Save or load file failed

……………………………………………… 64

6.13 Get dark current for a long time

…………………………………… 65

6.14 No backlight on LCD Display

………………………………………

66

6.15 Backlight OK, but nothing display on LCD Display

……………… 67

6.16 “Energy low” warning after blanking

………………………………

68

6.17 “Energy too low” warning

…………………………………………… 69

6.18 No DC +12V on PCB SST8.412.113

……………………………… 70

7. Maintenance

……………………………………………………

71

7.1 Working Environment

………………………………………………… 71

7.2 Replace Tungsten Lamp

……………………………………………… 71

7.3 Replace Deuterium Lamp

…………………………………………… 75

7.4 Replace Memory Battery

……………………………………………

77

7.5 Upgrade Firmware

……………………………………………………

78

7.6 Check Performance

…………………………………………………… 81

7.6.1 Prepare

…………………………………………………………………………… 81

7.6.2 Check Wavelength Accuracy

…………………………………………………

81

7.6.3 Check Stray Light

………………………………………………………………

82

7.6.4 Check Absorbance Accuracy

…………………………………………………

82

7.6.5 Check Dark Current

……………………………………………………………

82

7.6.6 Check Energy Profile

…………………………………………………………… 83

Appendix

……………………………………………………………

84

Order NO. List of UV-2800

………………………………………………… 84

Order NO. List of UV-2802, UV-2802PC

………………………………… 85

Order NO. List of UV-2802S, UV-2802PCS

……………………………… 86

Order NO. List of UV-3802

………………………………………………… 87

Order NO. List of UV-4802

………………………………………………… 88

Introduction

1. Introduction

The SQ series Spectrophotometer are available in five models, the model

UV-2800, UV-2802, UV-2802S, UV-3802 and UV-4802. This series products cover the Ultra Violet and Visible wavelength regions from 190nm to1100nm.

The analytical grating system has 1200 grooves/mm for high spectral dispersion. The models UV-2800, UV-2802,UV-2802PC, UV-2802S and UV-2802PCS are Single Beam, the model UV-3802 is Split Beam and the model UV-4802 is Double

Beam.

The SQ series Spectrophotometer used Double CPU, RTOS, High Precision

A/D and

320

×240 dot matrix LCD Display(320X240)

. All of these made the instruments have excellent performance and good stability.

Figure 1.1 Model UV-2800

- 1 -

Figure 1.2 Model UV-2802, UV-2802S

Introduction

Figure 1.3 Model UV-2802PC, UV-2802PCS

Figure 1.4 Model UV-3802

- 2 -

Figure 1.5 Model UV-4802

Introduction

- 3 -

2. Specifications

Specifications

- 4 -

3. Layout

3.1 Layout of UV-2800

1. Cooling Fan

3. Tungsten Lamp(W Lamp)

5. D2/W Power Supply PCB

7. Transformer

9. Lamp Limit Switch

11. Deuterium Lamp(D2 lamp)

13. Monochromator

15. Movable Nut

17. Sine Bar

19. Motor2(Wavelength)

21. Photo-couple1(Wavelength)

23. Shield Cover

25. Motor3(Filter)

27. Condenser Lens

29. Sample Compartment

31. Lamp Source Room

33. Light Blocking Plate

35. Power Distributing PCB

37. Main CPU PCB

39. LCD Screen(320x240 dots)

41. Sample Compartment Lid

43. Upper case

45. Viewing Hole

47. 232 Serial Port Interface

49. Power Switch

51. 110V/220V Selector

Layout

2. Concave Mirror

4. Heat Radiator

6. Motor1(Lamp Change)

8. Mirror1(Lamp Change)

10. Light Blocking Shelf

12. Entrance Slit

14. Monochromator Cover

16. Screw Thread Pole

18. Drive PCB

20. Synchro-Strap

22. Pull-Push Rod

24. Photo-couple2(Filter)

26. Amplifier PCB

28. 4-cell Sample Holder

30. Slave CPU PCB

32. Auto-cell Changer Connector

34. Rectifier

36. Switch Power Supply

38. Program Switch for Software Update

40. Keypad

42. Transferring PCB(In Upper Case)

44. Viewing Plate

46. Contrast Potentiometer for LCD

48. Printer Interface

50. Power Socket with Fuse with Fuse

- 5 -

17 18

Layout

Figure 3.1 Layout of UV-2800 Inside Top View

- 6 -

Layout

Figure 3.2 Layout of UV-2800 Inside Bottom View

- 7 -

Layout

Figure 3. 3 Layout of UV-2800 Top View

- 8 -

Layout

230V

Figure 3. 4 Layout of UV-2800 Back View

- 9 -

Layout

3.2 Layout of UV-2802(S), UV-2802PC(S)

1. Cooling Fan

3. Tungsten Lamp(W Lamp)

5. D2/W Power Supply PCB

7. Transformer

9. Lamp Limit Switch

11. Deuterium Lamp(D2 lamp)

13. Monochromator

15. Sine Bar

17. Movable Block

19. Gear

21. Photo-coupler1(Wavelength)

23. Auto-cell Changer Connector

25. Photo-coupler2(Filter)

27. Shield Cover

29. Condenser Lens

31. Slit Switch Equipment *

33. Photo-coupler4(Slit) *

35. Lamp Source Room

37. Light Blocking Shelf2

39. Power Distributing PCB

41. Main CPU PCB

43. LCD Display(320X240) **

45. Sample Compartment Lid

47. Upper Case

49. Viewing Hole

51. 232 Serial Interface

53. Power Switch

55. 110V/220V Selector

2. Concave Mirror

4. Heat Radiator

6. Motor1(Lamp Change)

8. Mirror1(Lamp Change)

10. Light Blocking Shelf1

12. Entrance Slit

14. Fortified Board

16. Screw Thread Pole

18. Wavelength Limit switch

20. Motor2(Wavelength)

22. Drive PCB

24. Pull-Push Rod

26. Motor3(Filter)

28. Amplifier PCB

30. Sample Compartment

32. 4-Cell Sample Holder

34. Motor4(Slit) *

36. Slave CPU PCB

38. Rectifier

40. Switch Power Supply

42. Program Switch for Software Update

44. Keypad **

46. Transferring PCB(In Upper Case) **

48. Viewing plate

50. Contrast Potentiometer for LCD

52. Printer Interface

54. Power Socket with Fuse

Note: * Only for UV-2802S, UV-2802PCS

** Only for UV-2802, UV-2802S

- 10 -

Layout

Figure 3.5 Layout of UV-2802, UV-2802PC Inside Top View

- 11 -

Layout

Figure 3.6 Layout of UV-2802S, UV-2802PCS Inside Top View

- 12 -

Layout

Figure 3.7 Layout of UV-2802(S), UV-2802PC(S) Inside Bottom View

- 13 -

Layout

Figure 3.8 Layout of UV-2802(S) Top View

- 14 -

Layout

230V

Figure 3.9 Layout of UV-2802(S) , UV-2802PC(S) Back View

- 15 -

3.3 Layout of UV-3802

1. Cooling Fan

3. Tungsten Lamp(W Lamp)

5. D2/W Power Supply PCB

7. Transformer

9. Lamp Limit Switch

11. Deuterium Lamp(D2 lamp)

13. Monochromator

15. Sine Bar

17. Movable Block

19. Gear

21. Photo-coupler1(Wavelength)

23. Auto-cell Changer Connector

25. Photo-coupler2(Filter)

27. Shield Cover

29. Condenser Lens

31. Light Distribute Room

2. Concave Mirror

4. Heat Radiator

6. Motor1(Lamp Change)

8. Mirror1(Lamp Change)

10. Light Blocking Shelf1

12. Entrance Slit

14. Fortified Board

16. Screw Thread Pole

18. Wavelength Limit switch

20. Motor2(Wavelength)

22. Drive PCB

24. Pull-Push Rod

26. Motor3(Filter)

28. Main Amplifier PCB

30. Sample Compartment

32. 4-Cell Sample Holder

33. Condenser Lens

35. Lamp Source Room

37. Shield

39. Rectifier

34. Slave Amplifier PCB

36. Slave CPU PCB

38. Light Blocking Shelf2

40. Power Distributing PCB

41. Switch Power Supply 42. Main CPU PCB

43. Program Switch for Software Update 44. LCD Display(320X240)

45. Keypad

47. Transferring PCB(In Upper Case)

46. Sample Compartment Lid

48. Upper Case

49. Viewing plate

51. Contrast Potentiometer for LCD

53. Printer Interface

55. Power Socket with Fuse

50. Viewing Hole

52. 232 Serial Interface

54. Power Switch

56. 110V/220V Selector

Layout

- 16 -

Layout

Figure 3.10 Layout of UV-3802 Inside Top View

- 17 -

Layout

Figure 3.11 Layout of UV-3802 Inside Bottom View

- 18 -

Layout

Figure 3.12 Layout of UV-3802 Top View

- 19 -

Layout

230V

Figure 3.13 Layout of UV-3802 Back View

- 20 -

3.4 Layout of UV-4802

1. Cooling Fan

3. Tungsten Lamp(W Lamp)

5. D2/W Power Supply PCB

7. Transformer

9. Lamp Limit Switch

11. Deuterium Lamp(D2 lamp)

13. Monochromator

15. Sine Bar

17. Movable Block

19. Gear

21. Photo-coupler1(Wavelength)

23. Auto-cell Changer Connector

25. Photo-coupler2(Filter)

27. Shield Cover

29. Condenser Lens

31. Light Distribute Room

33. Slave Amplifier PCB

35. Reference Holder

37. Slave CPU PCB

39. Power Distributing PCB

41. Main CPU PCB

43. LCD Display(320X240)

45. Sample Compartment Lid

47. Upper Case

49. Viewing Hole

51. 232 Serial Interface

53. Power Switch

55. 110V/220V Selector

Layout

2. Concave Mirror

4. Heat Radiator

6. Motor1(Lamp Change)

8. Mirror1(Lamp Change)

10. Light Blocking Shelf1

12. Entrance Slit

14. Fortified Board

16. Screw Thread Pole

18. Wavelength Limit switch

20. Motor2(Wavelength)

22. Drive PCB

24. Pull-Push Rod

26. Motor3(Filter)

28. Main Amplifier PCB

30. Sample Compartment

32. Sample Holder

34. Lamp Source Room

36. Light Blocking Shelf2

38. Rectifier

40. Switch Power Supply

42. Program Switch for Software Update

44. Keypad

46. Transferring PCB(In Upper Case)

48. Viewing plate

50. Contrast Potentiometer for LCD

52. Printer Interface

54. Power Socket with Fuse

- 21 -

Layout

- 22 -

Figure 3.14 Layout of UV-4802 Inside Top View

Layout

- 23 -

Figure 3.15 Layout of UV-4802 Inside Bottom View

Layout

- 24 -

Layout

Figure 3.16 Layout of UV-4802 Top View

- 25 -

Layout

230V

Figure 3.17 Layout of UV-4802 Back View

- 26 -

Optical

4. Optical System

The optical system of SQ series Spectrophotometers are adopted littrow type with 1200lines/mm grating, UV-2800, UV-2802,UV-2802PC, UV-2802S and

UV-2802PCS are Single Beam, the optical system schematic diagram are shown in

4.1. UV-3802 and UV-4802 are Split Beam and Double Beam, the optical system schematic diagram are shown in 4.2 and 4.3.

4.1

Incidence of Light

The neutral light coming from the Tungsten lamp or the Deuterium lamp is focused at the entrance slit of the monochrometer by the concave mirror.

4.2

Dispersion of Light

The light passed through the entrance slit , reflected by the collimating mirror to analytical grating. The light is spectrally dispersed by the grating and reflected collimating mirror to the exit slit.

4.3

Exit of Light

4.3.1 Exit of the Single Beam (UV-2800, UV-2802(S), UV-2802PC(S))

The light from the exit slit, through the condenser lens focused on the sample, continue pass through the sample, the light finally to the photodetector. Shown in Figure 4.1.

4.3.2 Exit of the Split Beam (UV-3802)

From the exit slit, the light was separated two path by the mirror, the main light focused on the sample after it passed though the condenser lens, continue pass through the sample, the light finally to the photodetector on the main Amplifier PCB. The slave light passed though the condenser lens to the photodetector on the Slave Amplifier PCB. Shown in Figure 4.2.

4.3.3

Exit of the Double Beam (UV-4802)

From the exit slit, the light was separated two paths by the mirror, the main light focused on the sample after it passed though the condenser lens, continue pass through the sample, the light finally to the photodetector on the main Amplifier PCB. The slave light focused on the 2nd exit slit in the slave light path, then focused on the reference after it passed though the slave condenser lens, continue pass through the reference, the light finally to the photodetector on the slave Amplifier PCB.

Shown in Figure 4.3.

- 27 -

Optical

Figure 4.1 Optical System schematic diagram of UV-2800,

UV-2802, UV-2802S, UV-2802PC, UV-2802PCS

- 28 -

Optical

Figure 4.2 Optical System schematic diagram of UV-3802

- 29 -

Optical

Figure 4.3 Optical System schematic diagram of UV-4802

- 30 -

5. Electronic System

Electronic System

Power Supply Socket

(3EGG1-2)

Red

Blk

Yel/Grn

Switch Power

(T-60B)

Red

Red

Wht

Wht

Yel/Grn

Wht

Org

Blk

Red

Power Switch

(R220-22C-11D)

Red

Blk

GND

115V/230 Selector

(L21-22A1)

PCB

(SST8.411.130A)

PCB

(SST8.412.113)

Brw

Org

PCB

(SST8.413.104A)

Connector

(4508A)

Wht,Brw,Org,Red

Transformor

Yel/Grn

PCB

(SST8.411.128A)

PCB

(SST8.417.100)

PCB

(SST8.412.314A)

LCD

PCB

(SST8.414.103) PCB

(SST8.417.102)

Keypad

Figure 5.1 Connection of UV-2800

- 31 -

Electronic System

Power Supply Socket

(3EGG1-2)

Red

Blk

Yel/Grn

Switch Power

(T-60B)

Red

Red

Wht

Wht

Yel/Grn

Wht

Org

Blk

Red

Power Switch

(R220-22C-11D)

Red

Blk

GND

115V/230 Selector

(L21-22A1)

PCB

(SST8.411.130A)

PCB

(SST8.412.113)

Brw

Org

PCB

(SST8.413.104A)

Connector

(4508A)

Wht,Brw,Org,Red

Transformor

Yel/Grn

PCB

(SST8.411.128A)

PCB

(SST8.417.100)

PCB

(SST8.412.314A)

LCD

PCB

(SST8.414.104)

Keypad

Only for UV-2802

Figure 5.2 Connection of UV-2802, UV-2802PC

- 32 -

Electronic System

Power Supply Socket

(3EGG1-2)

Red

Blk

Yel/Grn

Switch Power

(T-60B)

Red

Red

Wht

Wht

Yel/Grn

Wht

Org

Blk

Red

Power Switch

(R220-22C-11D)

Red

Blk

GND

115V/230 Selector

(L21-22A1)

PCB

(SST8.411.130A)

PCB

(SST8.412.113)

Brw

Org

PCB

(SST5.413.104A)

Connector

(4508A)

Wht,Brw,Org,Red

Transformor

Yel/Grn

PCB

(SST8.411.128A)

PCB

(SST8.417.100)

PCB

(SST8.412.314A)

LCD

PCB

(SST8.414.104)

Keypad

Only for UV-2802S

Figure 5.3 Connection of UV-2802S, UV-2802PCS

- 33 -

Electronic System

Power Supply Socket

(3EGG1-2)

Red

Blk

Yel/Grn

Switch Power

(T-60B)

Red

Red

Wht

Wht

Yel/Grn

Wht

Org

Blk

Red

Power Switch

(R220-22C-11D)

Red

Blk

GND

115V/230 Selector

(L21-22A1)

PCB

(SST8.411.130A)

Brw

Org

PCB

(SST8.413.104A)

PCB

(SST8.412.113)

Connector

(4508A)

Wht,Brw,Org,Red

Transformor

Yel/Grn

PCB

(SST8.413.103A)

PCB

(SST8.411.128A)

PCB

(SST8.417.100)

PCB

(SST8.412.314A)

LCD

PCB

(SST8.414.104)

Keypad

Figure 5.4 Connection of UV-3802

- 34 -

Electronic System

Power Supply Socket

(3EGG1-2)

Red

Blk

Yel/Grn

Switch Power

(T-60B)

Red

Red

Wht

Wht

Yel/Grn

Wht

Org

Blk

Red

Power Switch

(R220-22C-11D)

Red

Blk

GND

115V/230 Selector

(L21-22A1)

PCB

(SST8.411.130A)

Brw

Org

PCB

(SST8.413.104A)

Connector

(4508A)

PCB

(SST8.413.104A)

Wht,Brw,Org,Red

Yel/Grn

PCB

Transformor

(SST8.411.128A)

PCB

(SST8.412.113)

PCB

(SST8.417.100)

PCB

(SST8.412.314A)

LCD

PCB

(SST8.414.104)

Keypad

Figure 5.5 Connection of UV-4802

- 35 -

5.1 Power Supply System

Electronic System

Figure 5.6 Power Supply schematic diagram of UV-2800, UV-2802, UV-2802PC,

UV-2802S, UV-2802PCS

- 36 -

Electronic System

Figure 5.7 Power Supply schematic diagram of UV-3802, UV-4802

The power supply system of the SQ series are made up of two parts, the transformer supplied for the light source as Tungsten lamp and Deuterium lamp, and the Switch Power Supply supplied for the control system as CPU, Driver …,

- 37 -

Electronic System and so on. The schematic diagrams are shown in figure 5.6 and 5.7.

5.1.1 Power Supply PCB(SST8.411.128A)

The layout of the Power Supply PCB is shown in figure 5.8

Figure 5.8 Layout of Power Supply PCB

The sockets description of the Power Supply PCB as follow:

Pin 1: DC +18V

Pin (2, 3): AC 15V

Pin (1, 2): AC 15V

Pin (1, 4): AC 18V

Pin (2, 5): AC 18V

Pin 3, 6: GND

Pin (1, 4): AC 250V

Pin (5, 6): AC 105V

Pin (1, 3): AC 6V

J6---To 2SC2625 of Deuterium Lamp

Pin 1: DC +46V

J7---To Deuterium Lamp

- 38 -

Pin 1: DC +118V(+131V)

Electronic System

Note: When staring the Deuterium lamp, the voltage refer in the ( ).

Pin 1: DC +11V

Pin 2: GND

J9---From Main CPU PCB

Pin 1, 2: DC +5V

Pin 3: DC +3.85V(+5V)

Pin 4: DC +5V(+3.85V)

Note: 1. If the voltage of pin 3 is +3.85V, the Deuterium lamps is on, or it is off.

2. If the voltage of pin 4 is +5V, the Tungsten lamps is on, or it is off.

J10---To 2SC2625 of Tungsten Lamp

Pin 1: DC +18V

J11---To Cooling Fan

Pin 1: DC +12V

5.1.2 Power Distributing PCB(SST8.411.130A)

The layout of the Power Distributing PCB is shown in figure 5.9

Figure 5.9 Layout of Power Distributing PCB

The sockets description of the Power Distributing PCB as follow:

J1---From Switch Power Supply

Pin 1: DC -12V

Pin 2, 6, 7: DC +5V

- 39 -

Pin 3, 4, 5: DC +12V

Pin 8, 9, 10: GND

J2---To (Main) Amplifier PCB

Pin 1: DC +9V

J3---To Transferring PCB(In Upper Case)

Pin (1, 3): AC 1200V

J4---To Main CPU PCB

Pin 1, 2: DC +12V

Pin 3, 4: GND

Pin 5, 6: DC +5V

J5---To Slave Amplifier PCB

Pin 1: DC +9V

Electronic System

5.2 Control and Drive System

The working principle as follow (shown in figure 5.10, 5.11 and 5.12):

1. User setup the “Test Parameters” by operating the Keypad. The main

CPU (on “Main CPU PCB”) command the motor to goto the setup wavelength, select the correct light source and filter;

2. Halogen or deuterium lamp to supply the light, the Monochromator to isolate the wavelength of interest and eliminate the unwanted second order radiation, the sample compartment to accommodate the sample solution;

3. The detector to receive the transmitted light and convert it to an electrical signal, the slave CPU (on “Slave CPU PCB”) control to amplifier the electrical signal and convert it to be digital signal, send it to main CPU;

4. The main CPU gets the digital signal, process and outputs the results

(display, print or send to PC, and so on).

- 40 -

Electronic System

Figure 5.10 Working Principle of UV-2800, UV-2802(S)

- 41 -

Electronic System

Figure 5.11 Working Principle of UV-3802

- 42 -

Electronic System

Figure 5.12 Working Principle of UV-4802

The layout of the main CPU PCB is shown in figure 5.13

- 43 -

Electronic System

Figure 5.13 Layout of Main CPU PCB

- 44 -

Electronic System

The sockets description of the main CPU PCB as follow:

CZ1---From Power Distributing PCB

Pin 1, 2: DC +12V

Pin 3, 4: GND

Pin 5, 6: DC +5V

CZ2---To Slave CPU PCB

Pin 1: Reset W78E52B on Slave CPU PCB

Pin 2: Transfer signal to W78E52B on Slave CPU PCB

Pin 3: Receive signal from W78E52B on Slave CPU PCB

Pin 4: GND

Pin 5: DC +5V

CZ3---To RS-232 Port

Pin 2: Transfer signal

Pin 3: Receive signal

CZ4---To Transferring PCB(In Upper Case)

Pin 1-8: Output to signal Keypad

Pin 9-12: Input signal from Keypad

CZ5---To Printer Port

Pin 1: Pulse for read the data for printer

Pin 2-9: Data bus

Pin 10: Error detect. +5V indicate the printer is OK

Pin 11: Busy detect. +5V indicate the printer is busy

Pin 12: Reset the printer

Pin 15,16: GND

CZ6---To Transferring PCB(In Upper Case)

Pin 1-4, 13: Control signal of LCD

Pin 5: DC +5V- -20.5V, contrast adjust

Pin 6: DC +5V

Pin 7: GND

Pin 8: DC -20.5V

Pin 9-12: Data bus

CZ7---To Power Supply PCB

Pin 1: DC +5V(+3.85V). +5V: Tungsten lamps is on

Pin 2: DC +3.85V(+5V). +3.85V Deuterium lamps is on

Pin 3, 4: DC +5V

Pin 1-4: Drive auto-sample Holder step motor

Pin 4,5,6: DC +5V

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Electronic System

J17---To Program Switch for Software Update

Pin 1: DC +5V (0V). +5V: instrument is working on “Test

Mode”, or it working on “Program Mode”.

Pin 1: DC -20.5V

Pin 2: DC +5V- -20.5V, contrast adjust

Pin 3: DC +5V

CN1---To Drive PCB

Pin 1-4: Control slit step motor

Pin 5: Slit Photo-coupler signal

Pin 6: Wavelength Photo-coupler and limit switch signal

Pin 8: Lamp limit switch signal

CN2---To Drive PCB

Pin 2: DC +5V

Pin 3-6: Control wavelength step motor

Pin 7-10: Control filter step motor

Pin 11-14: Control lamp Change step motor

5.2.2 Drive PCB(SST8.414.103 or SST8.414.104)

The layout of the Drive PCB is shown in figure 5.14, 5.15 and 5.16

Figure 5.14 Layout of UV-2800 Drive PCB

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Electronic System

Figure 5.15 Layout of UV-2802S,UV-2802PCS Drive PCB

Figure 5.16 Layout of UV-2802, UV-2802PC, UV-3802, UV-4802 Drive PCB

The sockets description of the Drive PCB as follow:

CN1---From Main CPU PCB

Pin 1-4: Control slit step motor

Pin 5: Slit Photo-coupler signal

Pin 6: Wavelength Photo-coupler and limit switch signal

Pin 8: Lamp limit switch signal

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Electronic System

CN2--- From Main CPU PCB

Pin 2: DC +5V

Pin 3-6: Control wavelength step motor

Pin 7-10: Control filter step motor

Pin 11-14: Control lamp Change step motor

J4--To lamp Change step motor

Pin 1-4: Drive slit step motor

J5--To filter step motor

Pin 1-4: Drive filter step motor

J6--To wavelength step motor

Pin 1-4: Drive wavelength step motor

J7--To slit step motor

Pin 1-4: Drive slit step motor

J9---To filter Photo-coupler

Pin 2: DC +5V (0V). 0V: only at initiative position

J11---To slit Photo-coupler

Pin 2: DC 0V (+5V). +5V: only at initiative position

J12---To lamp limit switch

Pin 2: DC +5V (0V). 0V: only at initiative position

J14---To wavelength Photo-coupler and limit switch

Pin 2: DC 0V (+5V). +5V: when the mobile block at start or end point

The layout of the Transferring PCB(In Upper Case) is shown in figure

5.17

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Electronic System

Figure 5.17 Layout of Transferring PCB(In Upper Case)

The sockets description of the Transferring PCB(In Upper Case) as follow:

J3---From Power Distributing PCB

Pin (1, 3): AC 1200V

J31---To LCD back light

Pin (1, 2): AC 1200V

CZ4---From Main CPU PCB

Pin 1-8: Output signal to Keypad

Pin 9-12: Input signal from Keypad

CZ41---To keypad

Pin 1-8: Output signal to Keypad

Pin 9-12: Input signal from Keypad

CZ6---

From Main CPU PCB

Pin 1-4, 13: Control signal of LCD

Pin 5: DC +5V- -20.5V, contrast adjust

Pin 6: DC +5V

Pin 7: GND

Pin 8: DC -20.5V

Pin 9-12: Data bus

CZ61---

To LCD Display(320X240)

Pin 1-4, 13: Control signal of LCD

Pin 5: DC +5V- -20.5V, contrast adjust

Pin 6: DC +5V

Pin 7: GND

Pin 8: DC -20.5V

Pin 9-12: Data bus

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Electronic System

5.3 Sampling and Signal Processing System

The working principle as follow:

1. The photo-detector receives the light signal, converts it to be electrical signal

(analog signal);

2. Amplifier the analog signal, the multiple is control by the CPU on the Slave

CPU PCB (W78E52B);

3. The A/D converts analog signal to be digital signal;

4. The Slave CPU send the digital signal to the main CPU.

The layout of the Amplifier PCB of UV-2800, UV-2802, UV-2802PC,

UV-2802S and UV-2802PCS (R17—R20 of UV-2802S and UV-2802PCS are different from others) is shown in figure 5.18, UV-4802 is shown in figure

5.19:

Figure 5.18 Layout of UV-2800, UV-2802, UV-2802S,

UV-2802PC, UV-2802PCS, UV-3802 Amplifier PCB

The sockets description of the Amplifier PCB of UV-2800, UV-2802(S),

UV-3802 as follow:

J1---From Power Distributing PCB

J2---From Slave CPU PCB

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Electronic System

Pin 2: Analog signal from pin 6 of OP07

Pin 3-7: Control amplifier multiple from Slave CPU PCB

The amplifier multiple of UV-2800, UV-2802(S) Amplifier PCB as follow:

Amplifier multiple

Pin 3 Pin 4 Pin 5 Pin 6 Pin 7

0 0 0 0 0 0

1 0 1 0 0 0

2 1 0 0 0 0

3 1 1 0 0 0

4 1 1 0 0 1

5 1 1 0 1 0

6 1 1 0 1 1

7 1 1 1 0 0

8 1 1 1 0 1

9 1 1 1 1 0

10 1 1 1 1 1

Note: 1. 0: Logic low (0V) 1: Logic high (+5V)

The amplifier multiple of UV-3802 Amplifier PCB as follow:

Amplifier multiple

Pin 3 Pin 4 Pin 5 Pin 6 Pin 7

0 1 1 0 0 0

1 1 1 0 0 1

2 1 1 0 1 0

3 1 1 0 1 1

4 1 1 1 0 0

5 1 1 1 0 1

6 1 1 1 1 0

7 1 1 1 1 1

8 1 1 1 1 1

9 1 1 1 1 1

10 1 1 1 1 1

Note: 1. 0: Logic low (0V) 1: Logic high (+5V)

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Electronic System

Figure 5.19 Layout of UV-4802 Amplifier PCB

The sockets description of the Amplifier PCB of UV-4802 as follow:

J1---From Power Distributing PCB

Pin 1: DC +9V

Pin 2: GND

Pin 3: DC -9V

J2---From Slave CPU PCB

Pin 4: Analog signal from pin 6 of OP07

Pin 6,8,10,12,14: Control amplifier multiple from Slave CPU

PCB

The amplifier multiple of UV-4802 Amplifier PCB as follow:

Amplifier multiple

Pin 6 Pin 8 Pin 10 Pin 12 Pin 14

0 1 1 0 0 0

1 1 1 0 0 1

2 1 1 0 1 0

3 1 1 0 1 1

4 1 1 1 0 0

5 1 1 1 0 1

6 1 1 1 1 0

7 1 1 1 1 1

8 1 1 1 1 1

9 1 1 1 1 1

10 1 1 1 1 1

Note: 1. 0: Logic low (0V) 1: Logic high (+5V)

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Electronic System

5.3.2 Slave Amplifier PCB(SST8.413.103A or SST8.413.104A)

Figure 5.20 Layout of UV-3802 Slave Amplifier PCB

The sockets description of the slave Amplifier PCB of UV-3802 as follow:

J1---From Power Distributing PCB

J2---From Slave CPU PCB

Pin 3, 4: Control amplifier multiple from Slave CPU PCB

The amplifier multiple of UV-3802 slave Amplifier PCB as follow:

Amplifier Pin 3 Pin 4

1 0 0

2 0 1

3 1 0

4 1 1

Note: 1. 0: Logic low (0V) 1: Logic high (+5V)

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Electronic System

Figure 5.21 Layout of UV-4802 Slave Amplifier PCB

The sockets description of the slave Amplifier PCB of UV-4802 as follow:

Pin 2: GND

Pin 3: DC -9V

J2---From Slave CPU PCB

Pin 4: Analog signal from pin 6 of OP07

Pin 6,8,10,12,14: Control amplifier multiple from Slave CPU

PCB

The amplifier multiple of UV-4802 slave Amplifier PCB as follow:

Amplifier multiple

Pin 6 Pin 8 Pin 10 Pin 12 Pin 14

0 1 1 0 0 0

1 1 1 0 0 1

2 1 1 0 1 0

3 1 1 0 1 1

4 1 1 1 0 0

5 1 1 1 0 1

6 1 1 1 1 0

7 1 1 1 1 1

8 1 1 1 1 1

9 1 1 1 1 1

10 1 1 1 1 1

Note: 1. 0: Logic low (0V) 1: Logic high (+5V)

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Electronic System

The layout of the slave CPU PCB is shown in figure 5.22, 5.23, 5.24.

Figure 5.22 Layout of UV-2800, UV-2802(S) Slave CPU PCB

Figure 5.23 Layout of UV-3802 Slave CPU PCB

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Electronic System

The sockets description of the slave CPU PCB of UV-2800, UV-2802(S),

UV-3802 as follow:

J1---From Main CPU PCB

Pin 1: Reset W78E52B

Pin 2: Receive signal from W77E58 on Main CPU PCB

Pin 3: Transfer signal to W77E58 on Main CPU PCB

Pin 4: GND

Pin 5: DC +5V

J2---To Slave Amplifier PCB

Pin 2: Analog signal from pin 6 of OP07

Pin 3,4: Control amplifier multiple of Slave Amplifier PCB

J3---To Amplifier PCB

Pin 2: Analog signal from pin 6 of OP07

Pin 3-7: Control amplifier multiple of Amplifier PCB

Figure 5.24 Layout of UV-4802 Slave CPU PCB

The sockets description of the slave CPU PCB of UV-4802 as follow:

J1---From Main CPU PCB

Pin 1: Reset W78E52B

Pin 2: Receive signal from W77E58 on Main CPU PCB

Pin 3: Transfer signal to W77E58 on Main CPU PCB

- 56 -

Pin 4: GND

Pin 5: DC +5V

J2-- To Slave Amplifier PCB

Electronic System

Pin 4: Analog signal from pin 6 of OP07

Control amplifier multiple of Slave

Amplifier PCB

J3--- To Amplifier PCB

Pin 4: Analog signal from pin 6 of OP07

Pin Control amplifier multiple of Slave

Amplifier PCB

- 57 -

6. Trouble Shooting

6.1 Tungsten lamp off

Trouble Shooting

- 58 -

6.2 Deuterium lamp off

Deuterium lamp off

Trouble Shooting

DC +49V on pin 2,3 of J7 on PCB

SST8.411.128A OK?

N

DC +0.7V between pin 2,3(B,E) and

DC +43V between pin 1,3(CE) of J6 on PCB SST8.411.128A OK?

Y

1.Solving the wiring from J7 to deuterium lamp

2.Replace deuterium lamp

Y

N

1.Solving the wiring from J6 to 2SC2625

2.Replace 2SC2625

Y

1. Solving the wiring of J5 on PCB SST8.411.128A

2. Replace transformer.

1. Solving the wiring of J4 on PCB SST8.411.128A

2. Replace transformer.

N

N

DC +3.85V on pin 3 of J9 on PCB

SST8.411.128A OK?

N

DC +3.85V on pin 2 of CZ7 on PCB

SST8.412.113 OK?

N

Replace PCB SST8.412.113

Y

Y

DC +46V on pin 1 of J6 on

PCB SST8.411.128A OK?

N

AC 5.5V between pin 1,2 of

J5 on PCB SST8.411.128A

OK?

Y

AC 250V between pin 1,2 and AC95V between pin 3,5 of J4 on PCB

SST8.411.128A OK?

Y

Replace PCB

SST8.411.128A

Solving the wiring from CZ7 to J9 on PCB

SST8.411.128A

- 59 -

6.3 Memory check failed

6.4 Printer check failed

Trouble Shooting

Note

It is recommended to connect the M501 to printer with

cable after both are powered on!

- 60 -

6.5 Filter check failed

Filter positioning failed

Trouble Shooting

Filter motor works OK?

Y

DC +1.2V on pin 3 of J9 on PCB

SST8.414.103(104) OK?

Y

The voltage level on pin 2 of J9 on PCB SST8.414.103 changes within 0— 3.2V during the filter positioning?

Y

1. Solving the wiring of CN1 on

PCB SST8.414.103(104)

2. Replace PCB

SST8.414.103(104)

3. Replace PCB SST8.412.113

N

N

N

1. Solving the wiring of J9 on

PCB SST8.414.103(104)

2. Replace photo-coupler.

DC +12V on pin 5 of J5 on

PCB SST8.414.103(104)

OK?

Y

1. Solving the wiring of filter motor

2. Solving the wiring of CN2 on PCB SST8.414.103(104)

3. Replace PCB

SST8.414.103(104)

4.Replace filter motor

5.Replace PCB

SST8.412.113

N

DC +12V on pin 1 of CZ1 on

PCB SST8.412.113 OK?

N

Refer 6.18

Y

1. Solving the wiring CN1 on

PCB SST8.414.103(104) to

PCB SST8.412.113

2. Replace PCB

SST8.414.103(104)

1. Solving the wiring of CN2 on PCB SST8.414.103(104)

2. Replace PCB

SST8.414.103(104)

3.Replace PCB

SST8.412.113

- 61 -

6.6 Light source check failed

Trouble Shooting

Light Source positioning failed

Light change motor works OK?

Y

N

DC +5V on pin 3 of J12 on PCB

SST8.414.103(104) OK?

Y

The voltage on pin 2 of J12 on

PCB SST8.414.103(104) is from

0— 5V when the limit switch (refer item 9 of layout on page 3) is pressed down

Y

1. Solving the wiring CN1 on PCB

SST8.414.103(104) to PCB

SST8.412.113

2. Replace PCB

SST8.414.103(104)

3. Replace PCB SST8.412.113

N

N

DC +12V on pin 5 of J4 on

PCB SST8.414.103(104)

OK?

Y

1. Solving the wiring of light change motor

2. Solving the wiring of CN2 on PCB SST8.414.103(104)

3. Replace PCB

SST8.414.103(104)

4.Replace light change motor

5.Replace PCB

SST8.412.113

N

DC +12V on pin 1 of CZ1 on

PCB SST8.412.113 OK?

N

Refer 6.18

Y

1. Solving wiring J12 on PCB

SST8.414.103(104) to lamp change limit switch.

2. Replace lamp change limit switch.

1. Solving the wiring of CN2 on PCB SST8.414.103(104)

2. Replace PCB

SST8.414.103(104)

3.Replace PCB

SST8.412.113

1. Solving the wiring CN1 on

PCB SST8.414.103(104) to

PCB SST8.412.113

2. Replace PCB

SST8.414.103(104)

- 62 -

6.7 Auto-cell check failed

Auto-cell holder positioning failed

Trouble Shooting

Auto-cell holder motor works OK?

Y

The voltage level on pin 2 of J8 on

PCB SST8.412.113 changes within

0— 3.2V during the filter positioning?

N

1. Solving the wiring from auto-cell holder to auto-cell holder connector

2. Solving the wiring from auto-cell holder connector to J8 on PCB

SST8.412.113

3. Replace auto-cell holder photocoupler

Y

N

DC +12V on pin 1 of CZ1 on

PCB SST8.412.113 OK?

Y

1. Solving the wiring from auto-cell holder to auto-cell holder connector

2. Solving the wiring from auto-cell holder connector to

J3 on PCB SST8.412.113

3. Replace auto-cell holder motor

4.Replace PCB

ST8.412.113

N

Refer 6.18

1. Replace IC 8255 of U17 on PCB

SST8.412.113

2. Replace PCB ST8.412.113

- 63 -

Trouble Shooting

6.8 Slit check failed(Only for UV-2802S, UV-2802PCS)

- 64 -

Trouble Shooting

6.9 Search 656.1nm failed

(“D2 Energy too low” display on top side of right hand corner of the screen after finishing “Reset

WL” )

Search 656.1nm failed

Anything block off the beam in the sample compartment?

N

Deuterium lamp on?

Y

Light Source positioning OK?

Y

Filter positioning OK?

Y

Wavelength motor works OK?

Y

1. Solving the wiring of J2 on

PCB SST8.413.104A

2. Replace PCB

SST8.413.104A

3. Replace PCB

SST8.412.314A

Y

N

N

N

N

Take it away

Refer 6.2

Refer 6.6

Refer 6.5

1. Solving the wiring of wavelength motor

2. Replace PCB

SST8.414.103(104)

3. Replace PCB SST8.412.113

4. Replace wavelength motor

- 65 -

6.10 “Please restart” warning

“ Please restart” display on top right corner on LCD screen

Trouble Shooting

The slice on the screw thread pole stay in the photo-coupler?

Y

1. Solving the wiring of J14 on

PCB SST8.414.103(104).

2. Solving the wiring of CN2 on

PCB SST8.414.103(104).

3. Replace photo-coupler.

4. Replace PCB

SST8.411.103(104).

5. Replace IC 8255 of U17 on

PCB SST8.412.113.

6. Replace PCB ST8.412.113.

6.11 “Battery low” warning

N

1. Solving the wiring of limit switch to photo-coupler.

2. Solving the wiring of J14 on

PCB SST8.414.103(104).

3. Replace limit switch.

6.12 Save or load file failed (keypad goes dead after the key “Save” or “Load” is pressed)

Save or load file failed

Restart instrument, press

“ 7” in “ Main Menu” , press

“ F1” and select

“ yes” ,” yes” to format file system

N

Replace U4 on PCB

SST8.412.113

- 66 -

6.13 Get dark current for a long time

Trouble Shooting

- 67 -

6.14 No backlight of LCD Display

Turn on, LCD display have no backlight

Trouble Shooting

AC 110V or 220V Power on?

Y

Power cord connect OK?

Y

Fuse OK?

Y

AC 110V/220V input of Switch

Power OK?

Y

+5V of Switch Power output OK?

Y

+5V of J1 on PCB SST8.411.130A

OK?

Y

AC 1200V of J3 on PCB

SST8.411.130A OK?

Y

AC 1200V of J3 on PCB

SST8.417.100 OK?

Y

1. Solving wiring from LCD display to J3 on SST8.417.100

2. Replace LCD display

N

N

N

N

N

N

N

N

Power on

Connect power cord

Replace fuse

Solving wiring from power socket to the switch power

Replace switch power

Solving wiring from J1 on

PCB SST8.411.130A to switch power

Replace PCB

SST8.411.130A

Solving wiring from J3 on

PCB SST8.411.130A to

SST8.417.100

- 68 -

Trouble Shooting

6.15 Backlight OK, but display nothing on LCD Display

- 69 -

6.16 “Energy low” warning after blanking

Trouble Shooting

Note: If measuring at less than 200nm or more than 1000nm,it is possible to appear “Energy low” warning!

- 70 -

6.17 “Energy too low” warning

Energy too low

Trouble Shooting

Anything block off the beam in the sample compartment?

N

The wavelength at UV area?

N

N

The reference or sample solution have high absorbency against air?

Y

Dilute reference or sample solution

Y

Y

Y

Take it away

Used quartz cuvette?

N

Replace cuvette

The reference or sample solution have high absorbency against air?

N

Deuterium lamp on

N

Refer 6.2

Tungsten lamp on

N

Refer 6.1

Filter positioning OK?

Y

Light source positioning

OK?

Y

Y

Replace tungsten lamp(Vis area) or deuterium lamp(UV area)

N

N

Refer 6.5

Refer 6.6

Y

- 71 -

6.18 No DC +12V on PCB SST8.412.113

Trouble Shooting

- 72 -

7. Maintenance

Maintenance

7.1 Working Environment

Placed the instrument on a clean non-vibrational table, the optimum environment as follow: the temperature is between 5

℃ to 35℃, the humidity is no more than 85%, avoid direct strong light and air flow, the voltage stability is greater than

±10% and the frequency stability is greater than ±1% of the power supply, no strong magnetic field near the instrument. Always allow the instrument to warm up for 20 minutes prior before do any test.

7.2 Replace Tungsten Lamp

CAUTION: HIGH VOLTAGE

1. Turn off and unplug the instrument.

2. Remove the cuvette holder rod by unscrewing the rod counterclockwise.

3. Remove the all screws around the sides of the spectrophotometer. Show in

Figure 7.1.

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Maintenance

Figure 7. 1 Remove the cover screws

4. Very carefully remove the cover of the instrument and place in right side of the instrument. Show in Figure 7.2.

Figure 7. 2 Remove the cover

- 74 -

Maintenance

HINT: If it is necessary to remove the cover from the right side of the instrument, carefully remove 3 connectors (CZ6, CZ4 and J3) on

PCB marked SST8.417.100 . Be sure to reconnect after replacing the lamp! Show in Figure 7.3.

Figure 7.3 Unplug J3, CZ4, CZ6

5. Remove the grey metal protection cover. Using screwdrivers remove the two top screws and the two bottom screws, and then place the protective cover to the side. Shown in Figure 7.4.

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Maintenance

Figure 7.4 Remove the Lamp Cover

6. Remove the lamp from the ceramic base.

7. Insert the new lamp (Figure 7.5), pushing it in as far as it will go.

Figure 7.5 Tungsten Lamp

8. Re-fit the grey metal protection cover, temporarily re-fit the main cover and fix with two screws, one each side.

9. Switch on and remove the grommet from the middle of the rear panel. You

- 76 -

Maintenance

can now look through the Viewing Hole and view the image of the lamp on the slit. Check the lamp alignment shown in Figure 7.6. If the image is not covering the slit, the lamp alignment needs adjustment. This requires running the SQ series without the covers, with high voltages accessible, and so should only be performed by a suitably qualified engineer.

If adjustment is required, remove the cover and grey protection cover and turn on the instrument. Adjust to make the image central on the slit.

Install the grey metal protection cover and instrument cover.

Focus on the slit

Figure 7.6 Adjust Tungsten Lamp

CAUTION: DO NOT HANDLE THE LAMP WITH BARE FINGERS. USE

TISSUE OR CLOTH WHEN HANDLING LAMP. The oil from your fingers can cause the lamp to burn out prematurely.

10. Re-fit all the screws around the sides of the spectrophotometer.

11. Install the gray metal protection cover and cover of instrument.

12. Re-set the Tungsten lamp usage time. Select Utility, lamp and re-set W lamp usage time.

7.3 Replace Deuterium Lamp

- 77 -

1. Do the same step as 1-5 of 7.2 (replace Tungsten Lamp).

Maintenance

2. Disconnecting the connector J7 on the PCB marked SST8.411.128.

Unscrew the screw that holds the lamp bracket to the instrument base. Pull the entire lamp and lamp holder assembly out. Shown in Figure 7.7.

Figure 7.7 Take off the Deuterium Lamp

3. Replace the pre-aligned lamp with a lamp (Figure 7.8) provided by UNICO or an authorized UNICO Service Provider. This comes pre-assembled with lamp socket.

CAUTION: THE LAMP MAY BE HOT! TAKE PRECAUTIONS TO PREVENT

POSSIBLE BURNS.

- 78 -

Maintenance

Figure 7.8 Deuterium Lamp

4. Reconnect the connector J7 to the PCB marked SST8.411.128.

5. Re-fit the grey metal protection cover. Temporarily re-fit the main cover and fix with two screws, one each side.

6. Switch on and remove the grommet from the middle of the rear panel. You can now look through the Viewing Hole and view the image of the lamp on the slit. Check the lamp alignment Figure 7.9. If the image is not covering the slit, the lamp alignment needs adjustment. This requires running the SQ series without the covers, with high voltages accessible, and so should only be performed by a suitably qualified engineer.

If adjustment is required, remove the cover and grey protection cover, put on

UV protection glasses and turn on the instrument. Adjust to make the image central on the slit, Figure 7.9.

CAUTION: Wear UV protection glasses when replacing deuterium lamp.

7. Install the grey metal protection cover and cover of instrument.

8. Re-fit all the screws around the sides of the spectrophotometer.

9. Re-set the lamp usage time. Select Utility, lamp, and re-set D2 usage time.

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Maintenance

Focus on the slit

Figure 7.9 Adjust Deuterium Lamp

7.4 Replace Memory Battery

1. Turn the spectrophotometer upside down onto a padded surface. Remove the all screws around the sides of the spectrophotometer shown in Figure

7.10 and take away the bottom cover.

Figure 7. 10 Remove the bottom cover

- 80 -

Maintenance

2. There is a round lithium battery on the main CPU PCB. Shown in Figure

7.11where the red arrow is pointed. The battery model is CR1220 with 3V voltage from Panasonic.

Figure 7. 11 Battery Position

Note: Since the capacitor will only be able to maintain power for 5-6 minutes, we suggest you follow the procedure.

1) With the power unplugged, remove the bottom cover.

2) Locate the battery to be replaced on the board. DO NOT REMOVE IT

YET.

3) Make sure you have the replacement battery ready.

4) Turn on the spectrophotometer and keep it on for a few minutes.

5) Turn off the spectrophotometer.

6) Immediately after turning off the spectrophotometer, remove the old battery and insert the new one. This MUST be completed within 3 minutes of the spectrophotometer being turned off.

Note: Failure to follow the correct battery replacement procedure may result in saved data being lost and a possible PC software communication problem. It is recommended to transfer the data saved in the memory to computer with PC download software before replace battery.

3. Replace the bottom cover on the spectrophotometer, ensuring that all the star washers are in position.

7.5 Upgrade Firmware

1. Turn round spectrophotometer to find the switch shown in Figure 7.12 on the

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Maintenance

base that controls spectrophotometer to run in normal or upgrading status.

Convert the switch to upgrading status.

Figure 7. 12 Normal/Program Switch for Software Update

2. Connect spectrophotometer to the computer with the correct RS232 cable.

Note: It is recommended that you connect spectrophotometer to Comm1 of your computer.

3. Before updating the software, save or copy the folder “Update” UNICO provided to the disk C:/ of your computer. Usually there are five files in the folder “Update” which are respectively Flash-UNICO.exe, *.H00, *.H01, *.H02 and *.H03 (*.H00, *.H01, *.H02 and *.H03 is different base your instrument model.). After updating, two more files are created automatically, one is

UV2800.bin, another is serial.ini.

Note: The original serial number of spectrophotometer is stored in serial.ini.

4. Open the folder “Update” and double click on the icon “Flash- UNICO”.

5. The “System updating program for UNICO” screen will appear. Shown in

Figure 7.13.

Figure 7. 13 Upgrade Form

- 82 -

Maintenance

6. Click to select instrument model in Model Selection. Click on the small pull-down triangle on the right to select the comm. port (Comm1 default.)

There are 4 comm. ports available. It depends on which comm. port you connected between the computer and spectrophotometer.

7. Click on the ellipse icon “Load”, then only select the file “*.H00” in the folder

“Update”, click on “Open” to load.

8. Turn on spectrophotometer, the screen will display as below (Figure 7.14) and wait for upgrading.

Figure 7. 14 Upgrade State

9. Click on the button “Upload SN” in OEM information, the original serial number of spectrophotometer will appear in line 1 – line 3 and is stored in serial.ini . shown in Figure 7.15. If you do not have the original serial number, please contact UNICO.

Figure 7. 15 Upload SN

10. Click on the ellipse icon “flash” on the left to start.

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Maintenance

Figure 7. 16 Upgrading

Note: Do not click on any icon during the process of upgrading.

11. After beeping three times, the software is upgraded successfully, turn off the spectrophotometer, and then change the switch on the base of spectrophotometer to the normal position.

12. Turn on the spectrophotometer, it will run with the new software.

13. It is highly recommended that, following software upgrading, you select

“calibration system” after the self-testing process, shown in Figure 7.17.

Figure 7. 17 Calibrating

7.6 Check Performance

7.6.1 Prepare

1. Switch on and wait for 15 minutes. Ensure beam is clear.

2. Select “system calibration” yes.

3. When main menu appears at the end of calibration.

7.6.2 Check Wavelength Accuracy

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1. Prepare (Refer 7.6.1).

Maintenance

2. Press 3 to select “WL Scan”, press “F1” to setup, set the parameter as follow, press “Enter” to finished setup.

Scan from: 400

Scan to: 300

Scan Step: 0.1

Scan Speed: HI

3. Ensure beam is clear. Press “0Abs” to scan blank.

4. Insert holmium filter. Press “Start”.

5. When scan complete, press “F3”: Search, Press “▼”, record wavelength of holmium peak (specification: 360.9 ±1nm).

6. Press “ESC”, press “F1” to setup, set the parameter as follow, press

“Enter” to finished setup.

Scan from: 850

Scan to: 750

Scan Step: 0.1

Scan Speed: HI

7. Ensure beam is clear. Press “0Abs” to scan blank.

8. Insert didymium filter. Press “Start”.

9. When scan complete, press “F3”: Search, Press “▼”, record wavelength of didymium peak (specification: 807.1 ±1nm).

7.6.3 Check Stray Light

1. Prepare (Refer 7.6.1).

2. Press

λ”, input 340, press “Enter” to go to 340nm.

3. Ensure beam is clear. Press 1 to “Basic Mode” and automatic blanking.

4. Insert GG375 filter.

5. Record stray light at 340nm (specification >3A, <0.1%T).

7. Press

λ”, input 220, press “Enter” to go to 220nm.

8. Insert WG295 filter.

9. Record stray light at 220nm (specification >3A, <0.1%T).

7.6.4 Check Absorbance Accuracy

1. Prepare (Refer 7.6.1).

2. Fit neutral density filters, blank, 0.5A, 1A, 2A in cell changer and move blank into beam

3. Press

λ”, input 546, press “Enter” to go to 546nm.

4. Push filters into beam and record values. Compare the results with the calibrated absorbance (specification <±1%A, so <±0.005A at 0.5A,

<±0.010A at 1A, <±0.020A at 2A).

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7.6.5 Check Dark Current

1. Prepare (Refer 7.6.1).

2. Press 7 to select Utility.

Maintenance

page has a bar at the top and only the serial number below, instead of the diagnostic menu.

)

4. Press 5 to select “Check Dark Current” (even if “Check Dark Current” is not displayed). A table of Gain, Energy, will be produced (The minimum at Gain 0 should be greater than or equal to 1. The maximum at Gain 10 should be 2000 to 4000).

7.6.6 Check Energy Profile

1. Prepare (Refer 7.6.1).

2. Press 3 to select “WL Scan”, press “F1” to setup, set the parameter as follow, press “Enter” to finished setup.

Scan from: 1100

Scan to: 190

Scan Step: 0.5

Scan Speed: HI

3. Press 6, Press “Enter” to select ADEX: 1.

4. The wavelength will go to 1100nm and the energy profile will be produced.

5. Record the maximum value around 550nm (25% to 60% full scale).

Record the maximum value below 340nm (5% to 10% full scale).

(For 2nm bandwidth, the energy will be approximately half these values)

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Appendix

Order No. List of UV-2800

Appendix

PART NUMBER

SQ5-411-029

DESCRIPTION OR PCB NO.

SST8.411.128

SST8.411.128A

SQ5-411-030

SST8.411.130

SST8.411.130A

SQ5-412-021 SST8.412.113

SQ5-412-022

SST8.412.314

SST8.412.314A

SQ5-413-025 SST8.413.102

SQ5-414-012 SST8.414.103

SQ5-417-006 SST8.417.100

SQ5-415-007 SST8.417.101

SQ5-415-008 SST8.417.102

SQ0-001-001 Rectifier

SQ6-491-308 Motor2(Wavelength)

SQ6-491-309 Motor3(Filter)

SQ6-491-320 Transformer

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Order No. List of UV-2802, UV-2802PC

Appendix

PART NUMBER

SQ5-411-029

DESCRIPTION OR PCB NO.

SST8.411.128

SST8.411.128A

SQ5-411-030

SST8.411.130

SST8.411.130A

SQ5-412-021 SST8.412.113

SQ5-412-022

SST8.412.314

SST8.412.314A

SQ5-413-025 SST8.413.102

SQ5-414-013 SST8.414.104

SQ5-417-006 SST8.417.100

SQ5-415-007 SST8.417.101

SQ5-415-005

SST8.415.001-B

SST8.415.001-D

SQ0-001-001 Rectifier

SQ6-491-308 Motor2(Wavelength)

SQ6-491-309 Motor3(Filter)

SQ6-491-320 Transformer

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Order No. List of UV-2802S, UV-2802PCS

Appendix

PART NUMBER

SQ5-411-029

DESCRIPTION OR PCB NO.

SST8.411.128

SST8.411.128A

SQ5-411-030

SST8.411.130

SST8.411.130A

SQ5-412-021 SST8.412.113

SQ5-412-022

SST8.412.314

SST8.412.314A

SQ5-413-019 SST8.413.102

SQ5-414-014 SST8.414.104

SQ5-417-006 SST8.417.100

SQ5-415-007 SST8.417.101

SQ5-415-005

SST8.415.001-B

SST8.415.001-D

SQ0-001-001 Rectifier

SQ6-491-308 Motor2(Wavelength)

SQ6-491-309 Motor3(Filter)

SQ6-491-338 Motor4(Slit)

SQ6-491-320 Transformer

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Order No. List of UV-3802

Appendix

PART NUMBER

SQ5-411-029

DESCRIPTION OR PCB NO.

SST8.411.128

SST8.411.128A

SQ5-411-030

SST8.411.130

SST8.411.130A

SQ5-412-021 SST8.412.113

SQ5-412-023

SST8.412.314

SST8.412.314A

SQ5-413-025 SST8.413.102

SQ5-413-020

SST8.413.103

SST8.413.103A

SQ5-414-013 SST8.414.104

SQ5-417-006 SST8.417.100

SQ5-415-007 SST8.417.101

SQ5-415-005

SST8.415.001-B

SST8.415.001-D

SQ0-001-001 Rectifier

SQ6-491-308 Motor2(Wavelength)

SQ6-491-309 Motor3(Filter)

SQ6-491-320 Transformer

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Order No. List of UV-4802

Appendix

PART NUMBER

SQ5-411-029

DESCRIPTION OR PCB NO.

SST8.411.128

SST8.411.128A

SQ5-411-030

SST8.411.130

SST8.411.130A

SQ5-412-021 SST8.412.113

SQ5-412-025

SST8.412.314

SST8.412.314A

SQ5-414-013 SST8.414.104

SQ5-417-006 SST8.417.100

SQ5-415-007 SST8.417.101

SQ5-415-005

SST8.415.001-B

SST8.415.001-D

SQ0-001-001 Rectifier

SQ6-491-308 Motor2(Wavelength)

SQ6-491-309 Motor3(Filter)

SQ6-491-320 Transformer

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Key Features

  • Single Beam, Split Beam, Double Beam Configurations
  • Ultra Violet and Visible wavelength regions from 190nm to1100nm
  • High Precision A/D and 320×240 dot matrix LCD Display(320X240)
  • Double CPU
  • RTOS
  • High Stability

Frequently Answers and Questions

What are the different models in this series?
The SQ series includes five models: UV-2800, UV-2802, UV-2802S, UV-3802 and UV-4802.
What is the wavelength range of the SQ series instruments?
The SQ series instruments cover the Ultra Violet and Visible wavelength regions from 190nm to1100nm.
What is the difference between the single beam, split beam, and double beam configurations?
The UV-2800, UV-2802, UV-2802S, UV-2802PC and UV-2802PCS are Single Beam. The UV-3802 is Split Beam. The UV-4802 is Double Beam.
What is the optical system used in the SQ series?
The SQ series Spectrophotometers adopt the Littrow type with 1200 lines/mm grating.
What type of display do the SQ series instruments have?
The SQ series instruments feature a 320×240 dot matrix LCD Display(320X240).
What is the purpose of the RTOS?
The Real-Time Operating System(RTOS) ensures the stability and responsiveness of the instrument's control and data processing systems.

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