HUMAN HUMACOUNT PLUS, Hematology Analyzer Service Manual

Cat.-No.: 16400/2

Service Manual

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Hematology Analyzer

Revision 3.0

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1 INTRODUCTION......................................................................................................................................... 5

1.1

Name and serial number ..................................................................................................................... 5

1.2

Intended use ....................................................................................................................................... 5

1.3

Integrated software.............................................................................................................................. 5

2 FUNCTIONAL DESCRIPTION .................................................................................................................... 6

2.1

Main electronic parts of the analyzers.................................................................................................. 6

2.1.1

Cell-counter Probes....................................................................................................................... 7

2.1.2

HGB Head .................................................................................................................................... 7

2.1.3

Volume Opto Board....................................................................................................................... 8

2.1.4

Amplifier Boards............................................................................................................................ 8

2.1.5

Measurement & Pneumatic Interface Card (MPNIF) .................................................................... 10

2.1.6

Safe configuration E

2

PROM board (IDEPROM) ........................................................................... 11

2.1.7

High Voltage Board (HVB)........................................................................................................... 11

2.1.8

Graphic LCD Module................................................................................................................... 12

2.1.9

Keyboard Panel........................................................................................................................... 13

2.1.10

Start key & glowing LED.............................................................................................................. 13

2.1.11

Pressure & Liquid Sensor Board (PCPRO) .................................................................................. 13

2.1.12

Stepper Motor Opto Boards......................................................................................................... 14

2.1.13

Valve Driver Boards .................................................................................................................... 14

2.1.14

Main Board (MB4) ....................................................................................................................... 15

2.1.15

DimmPC Module ......................................................................................................................... 15

2.1.17

Switching Power Supply .............................................................................................................. 16

2.1.18

Floppy Disk Drive ........................................................................................................................ 16

2.1.19

Parallel and USB Printer Ports..................................................................................................... 16

2.1.20

Serial Port ................................................................................................................................... 16

2.2

Main mechanic and fluidic parts of the Analyzer................................................................................. 17

2.2.1

Aspirating tip ............................................................................................................................... 18

2.2.2

Dilutor ......................................................................................................................................... 18

H&V moving unit (XY) ............................................................................................................................... 19

2.2.4

Volume Meter Tube (xVM) – HUMACOUNT PLUS only............................................................... 20

2.2.5

Peristaltic Pump .......................................................................................................................... 20

2.2.6

Chamber(s*)................................................................................................................................ 21

2.2.7

Valves (1-5, 6-10*, 11-15) ........................................................................................................... 21

2.2.8

Washing head ............................................................................................................................. 21

2.2.9

Puffer reservoir ........................................................................................................................... 22

2.2.10

Tubes.......................................................................................................................................... 22

2.3

Assembled Analyzers........................................................................................................................ 23

2.3.1

HUMACOUNT PLUS Electronic Block ......................................................................................... 23

2.3.2

HUMACOUNT PLUS Mechanic and Fluidic Block........................................................................ 24

2.3.3

HUMACOUNT Electronic Block ................................................................................................... 25

2.3.4

HUMACOUNT Mechanical and Fluidic Block............................................................................... 26

3 OPERATION OF THE FLUIDIC SYSTEM ................................................................................................. 27

3.1

Waste Full Checking ......................................................................................................................... 27

3.2

Sampling process.............................................................................................................................. 28

3.3

Aspirating tip washing process........................................................................................................... 29

3.4

Diluting process................................................................................................................................. 30

3.5

Lysing process .................................................................................................................................. 31

3.6

WBC counting process ...................................................................................................................... 32

3.7

RBC counting process....................................................................................................................... 33

3.8

Washing processes ........................................................................................................................... 33

3.9

Cleaning process............................................................................................................................... 34

4 ADJUSTMENT.......................................................................................................................................... 35

4.1

Mechanical settings........................................................................................................................... 35

4.1.1

Opto wheel setting ...................................................................................................................... 35

4.1.2

Aspirating tip setting.................................................................................................................... 36

4.2

Hardware settings ............................................................................................................................. 36

4.2.1

Adjustment of the liquid detector sensitivity.................................................................................. 36

4.2.2

Amplifier offset setting ................................................................................................................. 37

5 CHECKING OF PROPER OPERATION.................................................................................................... 38

5.1

Self test............................................................................................................................................. 38

5.1.1

Self Test Screens........................................................................................................................ 38

5.1.2

Normal range of Self test parameters .......................................................................................... 39

5.1.3

Troubleshooting Guide for Self test.............................................................................................. 39

5.2

Service Menu .................................................................................................................................... 40

5.2.1

Entering to Service Menu ............................................................................................................ 40

5.2.2

Main Service Menu...................................................................................................................... 40

5.2.3

Edit Service Contact.................................................................................................................... 40

5.2.4

Device Information ...................................................................................................................... 40

5.2.5

Service Calibration ...................................................................................................................... 40

5.2.6

Software Settings ........................................................................................................................ 41

5.2.7

Service Testing Menu.................................................................................................................. 42

5.2.8

Valve Test Menu ......................................................................................................................... 42

5.2.9

Display and Keyboard Test.......................................................................................................... 43

5.2.10

Stress mode................................................................................................................................ 43

5.2.11

Miscellaneous settings ................................................................................................................ 43

5.2.12

Rescue Code for Multi-user Mode ............................................................................................... 43

6 SERVICE OPERATIONS .......................................................................................................................... 44

6.1

Opening of the instrument ................................................................................................................. 44

6.2

Removing/Installing DimmPC ............................................................................................................ 45

6.2.1

Removing DimmPC..................................................................................................................... 45

6.2.2

Installing DimmPC....................................................................................................................... 45

6.3

MDA (Monochrome Display Adapter) emulation mode ....................................................................... 45

6.4

Key BIOS settings for correct operation ............................................................................................. 46

6.4.1

BIOS Settings of DimmPC........................................................................................................... 46

6.4.2

Exit Menu.................................................................................................................................... 47

6.5

DOS Functions on the Instrument...................................................................................................... 47

6.6

Error messages................................................................................................................................. 48

6.6.1

Error code list.............................................................................................................................. 48

6.6.2

Message code list ....................................................................................................................... 51

2/77 Service Manual HUMACOUNT & HUMACOUNT PLUS

6.7

Problems related to Warning flags ..................................................................................................... 52

6.8

Possible Causes of Noise.................................................................................................................. 53

6.8.1

Contaminated reagent................................................................................................................. 53

6.8.2

Bad earth grounding.................................................................................................................... 53

6.8.3

External electrical noise............................................................................................................... 53

6.8.4

Internal noise sources ................................................................................................................. 54

7 MAINTENANCE........................................................................................................................................ 55

7.1

Weekly Maintenance by User ............................................................................................................ 55

7.1.1

Cleaning the washing head ......................................................................................................... 55

7.2

Periodic Maintenance by Service....................................................................................................... 55

7.2.1

Check Self test and Device statistics ........................................................................................... 55

7.2.2

Cleaning and Greasing Dilutor Block ........................................................................................... 55

7.2.3

Checking and Lubricating Dilutor Piston Tips............................................................................... 55

7.2.4

Cleaning and Lubricating Needle Moving Mechanics ................................................................... 55

7.2.5

Checking and Replacing Washing Head...................................................................................... 55

7.2.6

Checking and Replacing Peristaltic Pump Tube........................................................................... 55

7.2.7

Checking of the Power Supply..................................................................................................... 55

7.2.8

Bleaching of Fluidic System......................................................................................................... 55

8 SPARE PARTS......................................................................................................................................... 56

9 APPENDICES........................................................................................................................................... 61

9.1

Warning Flags ................................................................................................................................... 61

9.2

Serial Communication Protocol (V2.x) ............................................................................................... 62

9.2.1

General Description..................................................................................................................... 62

9.2.2

Format of Packages Sent by HUMACOUNT PLUS/HUMACOUNT .............................................. 63

9.2.3

Format of Acknowledge of the Receiver ...................................................................................... 63

9.2.4

Detailed Description of Packages ................................................................................................ 63

9.2.5

INIT package............................................................................................................................... 63

9.2.6

DATA package............................................................................................................................ 64

9.2.7

Transmission Example ................................................................................................................ 66

9.3

Recommended kit of tools ................................................................................................................. 67

9.4

HUMACOUNT PLUS tubing schematic.............................................................................................. 68

9.5

HUMACOUNT tubing schematic........................................................................................................ 69

9.6

HUMACOUNT PLUS Cabling Diagram .............................................................................................. 70

9.7

HUMACOUNT Cabling Diagram ........................................................................................................ 71

9.8

HUMACOUNT PLUS Amplifier, High voltage & HGB Boards ............................................................. 72

9.9

HUMACOUNT Amplifier .................................................................................................................... 73

9.10

HUMACOUNT PLUS Amplifier PCB .................................................................................................. 74

9.11

HUMACOUNT Amplifier PCB ............................................................................................................ 75

9.12

H&V, DIL, VM, LD Opto and Valve Boards......................................................................................... 76

9.13

Electronic part PCBs ......................................................................................................................... 77

9.14

Revision History ................................................................................................................................ 77

Service Manual HUMACOUNT & HUMACOUNT PLUS 3/77

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Since !"#$%&"'() */"2 and !"#$%&"'( have so much common characteristics, we cover both instruments in a common Service Manual. Information herein applies for both analyzers unless otherwise noted.

To be well up in the instruments, A+6:-6)B6:5)834-)C:;,:+)D:B67,++E to have the knowledge for servicing the instruments perfectly and avoid extra costs and wasting precious time.

This !"#$%&"'() */"2F!"#$%&"'() 26BG4D6) #:;,:+) contains the functional descriptions of both analyzers, the operation of the fluidic systems, the adjustments and settings and very important information for the Service Personnel about the service operations and possible problems.

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Every instrument has its own serial number, which is printed on the rear panel label and it can be read out from Device Information or from the Self test submenu. This serial number is write protected by HUMAN.

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!"#$%&"'() */"2F!"#$%&"'( hematology analyzers are fully automated cell counters for !"# $!%&' diagnostic use. The compact instruments were developed for small to medium size labs.

!"#$%&"'()*/"2 can process 60, !"#$%&"'( can process 30 samples per hour and they are intended to

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! WBC – LYM# - MID# - GRA# - LYM% - MID% - GRA% (three-part WBC differential)

! HGB – RBC – HCT – MCV – RDW – MCH – MCHC

! PLT – MPV – PCT – PDW

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The integrated software controls the instrument operations, displays, stores, recalls data, and allows the User to perform QC and calibration procedures and modify the user settings. The software version number can be read out from the Device Information or from the Self test submenu.

Instrument software (V1.7 and above) is absolutely ()*+,#-".#)*-/0,#it can detect what type of instrument is used and it will control the analyzer according to the hardware, without any user or service help. Every !"#$%&"'(

*/"2F!"#$%&"'( software version can be updated by the latest release available from HUMAN.


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!"#$%&"'()*/"2F!"#$%&"'( Hematology Analyzers consist of the following electronic parts:

5. Measurement & Pneumatic InterFace Card (MPNIF)

6. Safe configuration E

2

PROM board

7. High Voltage Board

8. Graphic LCD Display Module

10. Start key & glowing LED

11. Pressure & Liquid Sensor Board

12. Stepper Motor Opto Boards

13. Valve Driver Boards

14. Switching Power Supply

15. MB4 Main Board with DimmPC

16. Floppy Disk Drive

17. Parallel Port (optional USB printer port)

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Impedance method is used for determination of volume and number of cells. In this method a known volume of dilution is drawn through a small aperture. Constant current is passed through the aperture from one side to the other. When a cell passes through the aperture, it causes a change in resistance, which generates a voltage pulse.

The amplitude of the voltage pulse is proportional to the ratio of cell volume per aperture volume. This is used to determine the volume of cells. The number of cells can be obtained by counting the pulses.

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Assembled Cellcounter Probe Measuring tubes

Red rings mark measuring tubes:

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U-shaped metal fixing

Measuring tube Ground electrode assembly

The aperture is made of ruby and it is moulded into the measuring tube. The correct sealing around the aperture is very important for measurements.

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Hemoglobin head is placed at the bottom of the WBC chamber in the !"#$%&"'()*/"2R)and at the bottom of the measuring chamber in the !"#$%&"'(. It contains: light source (LED) at 540 nm and Photo Detector

(TSL235). The Photo Detector converts the light to frequency. The HGB concentration is a logarithmic function of this frequency measured by the FPGA circuit of the MPNIF card.

LED

Connection to the amplifier

Light sensor


!"#$%&"'()*/"2 makes four kinds of HGB measurements (diluent measurements are performed on diluent reagent):

! Sample dark measurement

! Sample light measurement

(before WBC counting)

(before RBC counting)

! Diluent light measurement

! Diluent dark measurement

(in WBC washing phase)

(immediately after the previous one)

The HGB result is calculated from these measurements by:

HGB " log [(CNT diluentlight

– CNT diluentdark

) / (CNT samplelight

– CNT sampledark

)]

!"#$%&"'( does not perform any dark measurements (it is always zero), so this equation is simpler:

HGB " log (CNT diluentlight

/ CNT samplelight

)

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It contains three opto switches which make possible to measure a constant volume of the diluted sample with a special float. The volume opto board is connected to the amplifier board through the VM connector (on the other side of this board).

OM1: Top opto

Measurement is made between OM1 and OM3 – no

LED on, (OM2: gate opto is for bubble error prevention).

OM3: Bottom opto

The upper LED is lit, when the float is between the

OM1 sensor.

The lower LED is lit, when the float is between the

OM2 sensor, but OM3 sensor is not

OM2: Gate opto

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!"#$%&"'()*/"2):;5)!"#$%&"'():CA+4746B)9J:B5-):B6)54776B6;8)*%.-, but there are a lot of common functional parts on them.

%JCCJ; functional parts: amplifier board includes its own voltage regulator, the connection interfaces for HGB head, for high voltage board and for MPNIF card.

In this board there is the current generator circuit, which works from 50 V measuring voltage (coming from high voltage board) and the probe (DC) voltage is amplified with a voltage follower (output: ELV). Nominal measuring current is UVW)X$.

The amplifier board includes the main 3-stage amplifier channel, which amplifies the input signal to the 0...5 V range (this is the input range of the A/D converter, which is on the MPNIF card). The offset voltage is set by P1 potentiometer by the manufacturer. $5Y,-8)836)J77-68)GJ+8:M6)J;+E)4;)D:-6)48)4-)J,8)J7)836)ZFP)[CS)B:;M6H

The test circuit makes possible to generate test pulses (with TEST and PLS signals through FETs) for checking the proper operation of the amplifier channel.


!"#$%&"'()*/"2):CA+4746B board includes also the connection (JP2) for volume opto limiter.

In this board there are two reed relays on the input side: IC10 can select between the two channels (RBC, WBC) with RSW signal; IC11 connects high voltage (from high voltage board) to the selected probe with HSW signal.

The DHON signal (from the MPNIF card) switches on the LED in the HGB head via a transistor (Q3), but the

Photo Detector in the HGB head is working continuously.

Connection. to the high voltage board

Reed relays

Connection to the MPNIF card (AMP and DIGIO)

!"#$%&"'():CA+4746B board includes only one input connector for the chamber.

In this board there is no relay in the input stage, but there are two opto switches (U1, U3: TLP627) to connect high voltage (from high voltage board) to the probe with HSW signal and isolate the input of the amplifier.

The RSW signal changes the gain (RBC, WBC) in the feed back of the second amplifier stage with U2 (MAX319) analog switch.

The DHON signal (from the MPNIF card) switches on the LED and also the Photo Detector in the HGB head via a transistor (Q3) that is why there is no dark HGB value in !"#$%&"'(.

Connection to the high voltage board

Connection to the MPNIF card

The other side of the amplifier boards includes special connectors for the chamber(s) and connector for the

HGB head (JP4).


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#*'>1 card incorporates measurement processing, and also the pneumatic, display and keyboard functions in one board.

In addition, this card has an #@$ (monochrome display adapter) emulation mode, which allows the Service

Person to use the LCD like a computer monitor or checking the proper operation of the instrument’s main board.

The controller card is connected to the main board through the ISA bus. After power on the card holds the main board in wait state (with –IOCHRDY signal) until the *>%)(micro-controller) configures the FPGA circuit from the configuration E

2

PROM located on the card, or from the safe E

2

PROM, located on the IDEPROM board. After that the 1*Q$ makes the I/O and memory address decoding and selects the actual part placed on the board:

! Video RAM for MDA (Monochrome Display Adapter) emulation and Graphics

! Card controller PIC

! Motor controller PICs (micro-controllers, controlling stepper motors)

The FPGA circuit also performs the main measurement data sampling and communication tasks. The board’s

A/D chip with its own sample/hold circuit makes the analog to digital conversion. After that the FPGA makes the data processing and stores the results in a temporary FIFO memory. The results are sent to the PC main board by DMA cycles.

With the internal registers of the FPGA the software can control the valves, the digital interface of the amplifier and the controller PIC. The PIC makes displaying (even greeting screen at start-up) and keyboard scanning, after the loading the configuration into FPGA.

CSM0: Connection to Horizontal motor

CSM1: Connection to Vertical motor

CSM2: Connection to Dilutor motor

CSK: Connection to keypad

CSV1: Connection to 1-5 valves



(Valve 16 = PUMP)

CSP: Connection. to the liquid

& pressure sensor board

CSD: Connection to the LCD module

CSA1, CSA2: Connection to the amplifier


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This board contains a 24FC256 serial E

2

PROM, which stores the FPGA’s configuration data, Safe greeting screen and identity information about the instrument. Keeping the hardware identity information (OEM information, model version: HUMACOUNT PLUS/HUMACOUNT) the IDEPROM makes possible to run the correct software (HUMACOUNT PLUS or HUMACOUNT) and store the instrument’s serial number.

The IDEPROM is connected to the MPNIF card and it is write protected.

The data of the normal configuration E

2

PROM on the MPNIF card can be modified by the software, allowing to change the FPGA functions. If there is any problem (e.g. power off) during this overwriting process, the instrument will start in “Safe mode” (this is displayed on the LCD) and the FPGA will be configured from the Safe

E

2

PROM (IDEPROM card).

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High Voltage Board generates the backlight voltage (300Vrms, 180 kHz) for CCFL (cold-cathode fluorescent lamp) of the LCD, the aperture cleaning voltage (150Vdc), and the measuring voltage (50Vdc). The high voltage board is connected to the system through the amplifier board and the MPNIF card.

This unit contains INVC191 inverter, which is a high voltage, high frequency circuit producing suitable voltage for

CCFL of the LCD.

Connection to amplifier

Connection to LCD lamp

The CFSW digital signal (from the MPNIF card) controls the high voltage board, if it is high (1) the inverter is off, if it is low (0) the inverter is on.

The MVON digital signal (from the MPNIF card) switches the measuring voltage (50 Vdc) on/off by an opto switch.

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This module contains 240x128 dots LCD display (HITACHI LMG7400PLFC or SP14N002), the HVB and this is the back panel of the keyboard module. LCD has a high voltage backlight lamp (the high voltage board generates the required voltage).

LCD Connection to MPNIF Connection to backlight lamp Connection to amplifier

High voltage board

Temperature sensor

Keyboard connection Ground connection

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In the !"#$%&"'( the HVB is fixed onto the LCD display holding frame:

Connection to LCD backlight lamp

HVB (Old version)

In the LCD module and on the MPNIF card there is a special temperature compensation circuit, which makes possible to use the LCD module in wide temperature ranges with the adjusted contrast.


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The analyzer has a 24-button foil keyboard including numerical keypad (0...9, .), cursor moving and OK buttons,

Delete and Help (?) keys and 6 function buttons, under the LCD display as it is shown in the figure below

(!"#$%&"'( keyboard panel):

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The Start key is a micro switch, connected to the MPNIF card (through the K&L ribbon cable). The glowing LED indicates the actual status of the analyzer and it has three colors: red, green and amber (See User’s Manual).

The LED has three pins and the actual color depends on the controlled pins. The LED is also connected to the

MPNIF card through the K&L ribbon cable.

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This board contains MPX5100AP calibrated pressure sensor, which can measure the pressure of air. It has two liquid detectors, for sensing diluent/cleaner and lyse reagents.

There are two potentiometers to adjust the sensitivity of both liquid sensors, but in different position for

!"#$%&"'() */"2 and !"#$%&"'( (see the pictures below). The potentiometer on the left is for the

Diluent/Cleaner, the one on the right is for the Lyse detector sensitivity. The PCPRO board is connected to the

MPNIF card through the P ribbon cable.

On the other side of the board there are two liquid detectors with indicator LEDs which show the actual state of the sensors: the corresponding LED is on if no reagent in the tube.

!"#$%&"'()*/"2)\)!"#$%&"'()*%*?&

Potentiometers

Pressure sensor

Connection to P cable

?E%'D"%&&0"%'&%2&#"'3#8%")3%&'1)V5101'WX'R')2*'53'E)&'3E%'&)1%'D#N%"'O#83)F%')&'3E%'#D3#

&%2&#"&'E)O%K'C#'2#3'$#22%$3'E5FE%"'O#83)F%'3E)2'WX'R'3#'3E5&'I#)"*'I%$)0&%'53'$)2'*)1)F%

3E%'D"%&&0"%'&%2&#"K


KH=H=K) 286AA6B)#J8JB)&A8J).J:B5-

There are three stepper motors in the system: Horizontal and Vertical motors, which make the movements of the aspirating tip moving carriage, and Dilutor motor, which moves the three syringes (macro, lyse, micro). The stepper motor opto boards make the connections between the motor controller PICs and motors, and have opto switches for the motor’s end and home positions. The actual status of the stepper motor’s optos is indicated by two LEDs on both stepper motor opto boards.

H&V motor opto board connects to the horizontal and vertical stepper motors and to the MPNIF card (through

HM and VM ribbon cables). On the other side of this board, there are four opto switches of the two stepper motors positions (home and end for both) and its own indicator LEDs.

H&V motor opto board Dilutor motor opto board

Dilutor motor opto board contains the opto switches of the dilutor motor, which moves the syringes in the fluidic system and the connection between the motor and the DM ribbon cable (from the MPNIF card). On the other side of the board there are two opto switches (home and end switches) and their own indicator LEDs.

KH=H=L) S:+G6)@B4G6B).J:B5-

In the !"#$%&"'()*/"2 there are three kinds of different valve driver boards:

! Valve board 1-5

! Valve board 6-10

! Valve board 11-15 & pump

In the !"#$%&"'( there are only two kinds of valve driver boards:

! Valve board 1-5

! Valve board 11-15 & pump

The Valve 11-15 driver board contains the

MO connection to the Pump unit.

The valve driver boards are connected to controller chips located on the MPNIF card through A, B and C ribbon cables. (In !"#$%&"'( there is no B cable). These boards control the valves in 5-valve sets.


KH=H=T) #:4;).J:B5)]#.T^

The analyzer works with a main board called #.T. Required settings of MB4 board and BIOS setup values are detailed in Chapter 6.

Floppy

Connector

DimmPC

ATX Power Supply

Connector

Keyboard

Connector

Li battery

Standby

LEDs

COM1 Printer

Connector

COM2

Power Switch Connector

Speaker

Connector

ISA slot for MPNIF card

#.T)9J:B5 contains the necessary connectors for the different resources, and an ISA slot for the MPNIF board.

There is a 3V Li-battery to feed Real-Time Clock and Calendar (RTCC) chip during power down.

1+JAAE)54-<)5B4G6 is connected to the MB4 with a ribbon cable. The required configuration of the floppy disk drive (boot sequence) can be set in the Boot priority section of the BIOS settings.

S456J) :5:A86B (MDA: Monochrome Display Adapter) and G456J) C6CJBE are placed on the MPNIF card. In

MDA mode the instrument displays the text monitor output on the LCD.

Two LEDs are indicating the state of power on MB4 main board:

1. Green LED is on, if external power is present (stand-by mode),

2. Yellow LED is on, if power is turned on.

>8N)G&'"%1#O%'%V3%"2)8'D#N%"H'#"'30"2'#77'D#N%"'&N53$E'#2'3E%'D#N%"'&0DD8G'57'G#0'"%1#O%

#"'"%D8)$%'$#1D#2%23&'+%KFK'C511!,H'.!:6;H'.(Y'1)52'I#)"*H'%3$K

KH=H=[) @4CC*%)#J5,+6

The MB4 board incorporates a credit-card sized PC, named @4CC*%c. The processor on the DimmPC is a

133MHz Pentium-class core, with 32Mbytes on-board RAM, and 32Mbytes on-board SanDisk. This is the HDD

(hard disk drive) of the analyzer, so instrument software with all user settings, calibration, database, etc. is stored on the DimmPC.

>#7!88):?#!;#%@2#A&-.2#B-&6#'=#C'"%&'"#18<2..2.#B'.+*2;#D8<E


Flash

BIOS

32 Mbytes RAM

Hard Disk

(SanDisk)

SanDisk controller

CMOS

EEPROM

Real-timeclock

On-board

SMPS

Super I/O

Edgeconnector

KH=H=_)

AMD Elan

SC520

CPU

KH=H=V) 2N48D34;M)*JN6B)2,AA+E

The analyzer works with a standard, 200 or 230VA ATX type PC switching power supply. The power supply has its own internal fuse and a built in fan. The most frequent problem is the malfunction of the fan, so check and clean it regularly. The power supply has a temperature sensor, which can detect overheating and shut down the power.

KH=H=U) 1+JAAE)@4-<)@B4G6

The built in Floppy Disk Drive makes possible to save data on floppy disks, and to install (or upgrade) the software.

I normal case (and for SW upgrade), the boot device priority should be Hard Drive then Floppy drive in BIOS

Setup. In this case, the analyzer starts up from SanDisk.

Boot Device Priority submenu – for Normal operation:

+ Hard Drive

+ Removable Devices

If you need to boot from Floppy disk, set up the following boot order. In this case it is possible to boot from an external floppy disk, containing bootable code (e.g. MS-DOS). You can refer to the floppy disk as A:, SanDisk will be C:.

Boot Device Priority submenu – for booting from a bootable floppy disk:

+ Removable Devices

+ Hard Drive

KH=H=`) *:B:++6+):;5)"2.)*B4;86B)*JB8-

The “Printer” port located on the rear panel is for external printers with parallel port.

The USB port is an option. If DimmPC is equipped with USB module, and at least SW V3.0 is installed the analyzer supports USB printer connection.

KH=HKW) 26B4:+)*JB8

The Serial port is a standard RS232 V24 port with the standard pin-out. COM1 – marked “Serial” can be used is useful for communication with a host computer.


KHK) #:4;)C6D3:;4D):;5)7+,454D)A:B8-)J7)836)$;:+EO6B

!"#$%&"'()*/"2F!"#$%&"'( Hematology Analyzers consist of the following mechanic and fluidic parts:

1. Dilutor

2. H&V moving unit

3. Volume meter tube (xVM) – only for !"#$%&"'()*/"2

4. Pump

5. Aspirating tip

6. Chamber(sc)

7. Valves (1-5, 6-10c, 11-15)

8. Washing head

9. Puffer reservoir

10. Tubing

S==)@4+d:-3

Micro

2

S=L)@4+'665+6

1

3

Macro

LDL

Dil M3

LYSE

1

S=T)/E-6d9D

2

3

Lyse

S=[)*,776B$4B

*

Pressure

Meter

LDL

WBC

HGB

S=K)@4+1+J:8

SU)$-Ad9D

2 1

3

2 1

S=W)@4+d9D

SV)1+J:8"A

3

S`)%+6:;6B

1 2

3

SL)1+J:8@JN;

2 1

3

RBC xV

M

M1

Hor

MIX

DILUENT

M2

Ver

CLEANER

Puffer

Reservoir

ST)@B:4;d9D

S[)*,776B SK)@B:4;?9D

S=)@B:4;#4e

S_)@B:4;d:-3

Pump

WASTE

Waste Full Sensor

M1 Stepper Motor LDL Liquid Detector 2-way Valve

Closed = Off

Open = On

1

3

2

<=.>,-=:?'!@=A 4*+!.!3#F3@28-%!3;

3-way

Valve

1-3 = Off

2-3 = On

<=.>,-=:? 4*+!.!3#F3@28-%!3;

S==)@4+d:-3

Micro

2

S=L)@4+'665+6

1

3

1

S=T)/E-6d9D

2

LDL

3

Lyse

Sensor

Macro

Dil M3

LYSE

Lyse

S=K)@4+%3:C96B

Diluent & Cleaner

Sensor

1

LDD

3

2

S=[)@4+$A6B8,B6

S[)%+6:;6B

M1

Hor

M2

Ver

*

Pressure

Meter

Puffer

Reservoir

SL).,99+6

HGB

S=)@B:4;%3:C96B

RBC/WBC

SK)@B:4;$A6B8,B6

DILUENT

CLEANER

Pump

2

3

1

ST)@B:4;*,776B

WASTE

LDx Liquid Detector

M1 Stepper Motor

2-way Valve

Closed = Off

Open = On

1

3

2

3-way

Valve

1-3 = Off

2-3 = On


KHKH=) $-A4B:84;M)84A

The aspirating tip is assembled in the H&V moving unit and it makes the sample aspirations. The right setting of the aspirating tip is necessary and very important (see Chapter Adjustments).

KHKHK) @4+,8JB

This unit is the same for both instruments (!"#$%&"'() */"2 and !"#$%&"'() and includes the dilutor stepper motor, the dilutor motor opto board, three syringes and the piston rods with special transmission. The dilutor motor opto board is on the other side of this unit.

The picture below shows the !"#$%&"'() */"2F!"#$%&"'( dilutor module with the three separated syringes.

The dilutor block is operated by a stepper motor, which is connected to the motor opto board. The motor opto board has home & end position opto switches. The optical

Lyse syringe

Macro syringe home/end position switches are operated by a small tag fixed on the moving part. The principle is the interruption of infra-red light emitted by the diode of the sensor. The control lights (LEDs) are showing the actual state of the sensors: if the light is on, the dilutor moving part reached the corresponding sensor.

Micro syringe

!"#$%&"'() */"2F!"#$%&"'( dilutor module works with separated syringes (glass for Macro and Lyse; plexiglass for Micro).

Macro syringe is for diluent reagent, Lyse syringe is for lyse reagent, and the Micro syringe is for precision sampling during measurement.

Control lights

(LEDs) for chackin the state of opto switches

Opto switches

.)523%2)2$%'&E#08*'I%'D"#O5*%*'3#'3E%'D5&3#2'35D&H'IG')DD8G52F'2%03")8'&585$#2'F"%)&%'3#'3E%

$#FF%*'%2*'#7'3E%'.)$"#')2*'@G&%'D5&3#2&H'I%3N%%2'3E%'&G"52F%')2*'3E%'35D'53&%87K'?E5&'N588

%2&0"%'#D35101'&%)852F')2*'8#2F%"'857%351%'#7'D5&3#2'35D&K

J"%)&52F' #7' 3E%' $#FF%*' 3")2&15&&5#2' D)"3&' +$#FNE%%8' )2*' $#FF%*' I)"/' &E#08*' I%' *#2%

"%F08)"8G'0&52F'1)$E52%'F"%)&%K

63'5&'"%$#11%2*%*'3#'$E%$S')2*'"%D%)3'F"%)&52F'#7'D5&3#2'35D&H')2*'3")2&15&&5#2'F%)"'%O%"G

G%)"H'#"')73%"'ZQQQQ'1%)&0"%1%23&K


KHKHL) !\S)CJG4;M),;48)]fg^

This unit is the same for both instruments (!"#$%&"'() */"2 and !"#$%&"'() and it contains the

Horizontal and Vertical stepper motors, H&V motor opto board, opto wheel and the special mounting.

This moving unit moves the aspirating tip holding carriage and in the assembled analyzer it includes the aspirating tip and the washing head.

Aspirating tip holder Home opto switch H&V motor opto board

Washing head housing Horizontal motor Vertical motor

The Vertical motor works with a special opto wheel for detecting home & end positions. See the adjustment section of this manual to place this wheel to the proper position.

J"%)&52F'#7'3E%'E#"5T#23)8BO%"35$)8'F05*52F'"#*&'&E#08*'I%'*#2%'"%F08)"8G'0&52F'[!E#3#"0I\H

)'!?;]UI)&%*'3E52'80I"5$)23K

63'5&'"%$#11%2*%*'3#'$E%$S')2*'"%D%)3'F"%)&52F'#7'F05*52F'"#*&'%O%"G'G%)"H'#"')73%"'ZQQQQ

1%)&0"%1%23&K


KHKHT) SJ+,C6)#686B)(,96)]eS#^)h)!"#$%&"'()*/"2)J;+E

The volume meter tube (xVM) is mounted on the volume opto board (see photo).

The tube contains a float, which is moved up by diluent flow before counting.

Volume Meter tube

Float

During counting, first it comes down, the measurement is started when the top LED goes off, and the movement of the float is proportional to the volume of WBC dilution measured.

Counting stops when bottom LED goes on.

RBC counting is performed when the float goes upwards (none of the LEDs are on).

?E%')""#N'#2'3E%'30I%'10&3'D#523'0DN)"*&'NE%2

"%D8)$52F'30I%K

C#'2#3'15V'0D'3E%'78#)3'*5"%$35#2'*0"52F'$8%)252F'#7'3E%'30I%H'I%$)0&%'3E%'$E)17%"52F'5&'2#3

&G11%3"5$)8H')2*'3E%'78#)3'1)G'I8#$S'3E%'30I%K'?E%'8#2FH'&1)88')2F8%'$E)17%"'10&3'7)$%'3#

3E%'I#33#1'#7'3E%'30I%K

KHKH[) *6B4-8:+84D)*,CA

The pump generates the regulated vacuum and drains the fluidic system. The pump is connected to the Valve

11-15 driver board (MO connection) and the control chip located on the MPNIF card, which drives this unit. The pump is operated from 12Vdc.

If the tube of the peristaltic pump becomes worn, it can be broken, causing Pressure error.

63'5&'"%$#11%2*%*'3#'$E%$S'3E%'&3)3%'#7'3E%'30I%H')2*'"%D8)$%'53'%O%"G'^'G%)"&H'#"')73%"'^Q'QQQ

1%)&0"%1%23&K

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For servicing the tube of the pump, open the peristaltic pump from its top (see picture) and remove the tube together with the white plastic side wall (see picture):

In case of damaged tubes, it can be replaced by a new one by opening the two metal locks located at the two ends of the tube (see picture).


KHKH_) %3:C96B]-c^

In the !"#$%&"'()*/"2 there are three different chambers made of polycarbon. The MIX chamber is for the first sample dilution. The RBC and the WBC chambers are mounted with the cell counter probes.

The chamber on the left is an !"#$%&"'()*/"2 MIX chamber, it is closed from the front and it is open at the top.

The chamber on the right is an !"#$%&"'() */"2

WBC chamber with the hole in the front for the cellcounter probe. It is closed at the top and it has the special WBC long PTFE draining connector.

Place for cellcounter probe

Long PTFE draining connector

In the !"#$%&"'( there is only one chamber for diluting and measuring the blood cells. This chamber is the same as the WBC chamber in the !"#$%&"'()*/"2 with the long draining connector but it is open at the top

– and it has an extender cap on it.

It is extremely important for both instruments that these chambers should have no damage or crack because it could cause erroneous measurements (noise).

KHKHV) S:+G6-)]=P[R)_P=WcR)==P=[^

There are two kind of pneumatic valves in the system:

Valve

Valves are assembled in a unit of five on the valve blocks.

!"#$%&"'()*/"2 contains 3 of these boards (15 valves), !"#$%&"'( has only 2 valve blocks (10 valves).

KHKHU) d:-34;M)36:5

Washing head is located at the bottom of the H&V moving unit and it is for cleaning the outer surface of the aspirating tip. This washing process is made by diluent reagent and the fluid is drained by the pump. The arrows on the picture show the direction of diluent flow.

Draining by the pump

Diluent reagent

63'5&'"%$#11%2*%*'3#'$E%$S'3E%'&3)3%'#7'3E%'N)&E52F'E%)*')3'"%F08)"'&%"O5$%'O5&53&K

,8%)2'#"'"%D8)$%'53'G%)"8GH'#"')73%"'ZQ'QQQ'1%)&0"%1%23&K


KHKH`) *,776B)B6-6BGJ4B

The puffer reservoir insures the stable vacuum during the measuring process. !"#$%&"'() */"2 and

!"#$%&"'( puffer reservoirs :B6 made by glass but in 54776B6;8 sizes. The figures of these units are shown on the assembled analyzers section of this manual.

KHKH=W) (,96-

The fluidic system contains tubes, which have different diameters and lengths and also made by different materials.

Reagent paths requiring perfect sealing are made of Tygon tubing, the draining circuit is made of silicon.

The Tubing Schematics Section contains the parameters of the tubes (length & diameter).

63'5&'"%$#11%2*%*'3#'$E%$S'3E%'&3)3%'#7'3E%'30I52F'`'%&D%$5)88G'8G&%'D)3E'`'"%F08)"8GH'*0"52F

&%"O5$%'O5&53&K a02')'$#1D8%3%'I8%)$E52F'$G$8%H'$E%$S'#"'"%D8)$%'30I52F')2*'753352F&'G%)"8GH'#"')73%"

ZQ'QQQ'1%)&0"%1%23&K


KHL) $--6C9+65)$;:+EO6B-

This section includes the photos of the assembled analyzers. There are two main parts for both instruments:

! The electronic block located on the left side

! The mechanic and fluidic block located on the right side and it is assembled to a stainless steal assembly plate, which makes also the separation from the electronic block

KHLH=) !"#$%&"'()*/"2)0+6D8BJ;4D).+JD<

This block contains the main electronic parts of !"#$%&"'() */"2, the cables and the main board holder plate.

Valve Block III.

Pressure and Reagent

Sensor Board

Interface connectors

MPNIF Card

IDEPROM Board

KHLHK)

Main Board (MB4)

Floppy disk drive

Power Supply


!"#$%&"'()*/"2)#6D3:;4D):;5)1+,454D).+JD<

This block includes the mechanic and fluidic parts (see 2.2) of !"#$%&"'() */"2 and it is located on the assembly plate. The analyzer has a door (with magnetic lock) on the right side, which makes easy to access the mechanic and fluidic system.

Sampling needle Puffer Reservoir H&V Moving Unit Reagent Sensors

Valve Block III.

Washing head

Measuring

Block

Valve Block II.

Valve Block I.

Dilutor Block Peristaltic Pump Reagent Inputs


KHLHL) !"#$%&"'()0+6D8BJ;4D).+JD<

This block contains the main electronic parts of !"#$%&"'(, the cables, and the main board holder on a rack system plate.

Power Supply LCD

Interface connectors

Main Board (MB4)

MPNIF Card

Power Switch

Speaker IDEPROM Board

Floppy disk drive


KHLHT) !"#$%&"'()#6D3:;4D:+):;5)1+,454D).+JD<

This block includes the mechanic and fluidic parts (see 2.2) of !"#$%&"'( and it is located on the assembly plate. The analyzer has a door (with magnetic lock) on the right side, which makes easy to access the mechanic and fluidic system.

Sampling needle Puffer Reservoir

Washing head

Measuring

Chamber

Reagent

Sensors

H&V Moving

Unit

Valve Block II.

Measuring

Block

Dilutor Block

Valve Block I.

Peristaltic Pump

Reagent

Inputs


L) &*0?$(>&')&1)(!0)1/">@>%)2g2(0#

This section describes the main fluidic steps of !"#$%&"'() */"2 and !"#$%&"'( measurement cycle.

The fluidic systems are totally different in the two instruments but they use the same modules (valves, tubes, chambers, dilutor, etc.) and from the point of the measurements they follow the same steps. For this reason we show the fluidic system processes for both instruments in the same logical blocks.

!"#$%&"'() */"2 and !"#$%&"'( Fluidic Schematics are shown in Section 2.2 of this manual. The following figures show the actual process and help to understand how the fluidic system works.

The following steps are introduced in this section: process

2. Aspirating tip washing process

5. WBC counting process

6. RBC counting process

The active pipe is filled with black color, beside the tube an arrow ( # ) shows the direction of the flow. The moving mechanic parts have a bigger arrow (!) indicating the direction of movement. Only the open (On) valves are mentioned in this section while all the other valves are closed (Off).

LH=) d:-86)1,++)%36D<4;M

There is a special pneumatic process in !"#$%&"'()*/"2, it is called d:-86)1,++)%36D<4;MH

Valve15 switches the measuring vacuum to the waste full sensor tube for a short period of time (10 msec). The pressure meter measures the decrease of the vacuum during this time. From this value the program can decide that the vacuum has aspirated fluid or air so the waste container is full or not.

In !"#$%&"'( there is no pneumatic)Waste Full Checking. It is counting the volume of the reagents used, and gives a message when this volume reaches the preset tank capacity. You can modify the volume of waste tank in the Service menu.

Generally it is recommended to set the volume of waste tank 2 liters less than the real capacity of the tank.


LHK) 2:CA+4;M)ABJD6--

56(7,(E6\(7,(&'(3$"R!&,D#"!(%+!"G(+*(8,++!(41%3,"(&4(143&$1#"!(82(#)"(143&$1#&'.(#&3W(Q)"(C&?$+(42$&'."(%1U"4(#)" aspirating while the M3 Dilutor motor moves down.

!"#$%&"'()*/"2(#)"$"(&4(1'+#)"$(41%3,&'.(3$+?"44(-)"'(56(7,(+*(3$&%1$2(!&,D#&+'(&4(143&$1#"!(82(#)" aspirating tip from the MIX chamber to the RBC chamber for the second (RBC) dilution.

Sample

Micro

2

S=L)@4+'665+6

1

3

LDL

Macro

Dil

M3

LYSE

1

S=T)/E-6d9D

2

3

Lyse

S=[)*,776B$4B

*

Pressure

Meter

S=K)@4+1+J:8

LDL

WBC

HGB

3

2 1

S=W)@4+d9D

SU)$-Ad9D

2 1

3

SV)1+J:8"A

S`)%+6:;6B

1 2

3

SL)1+J:8@JN;

2 1

3

RBC xV

M

Pump

Puffer

Reservoir

ST)@B:4;d9D

S[)*,776B

WASTE

Waste Full Sensor

SK)@B:4;?9D

M1

Hor

MIX

S==)@4+d:-3

S=)@B:4;#4e

S_)@B:4;d:-3

DILUENT

M2

Ver

CLEANER

! In dilution is aspirated by the aspirating tip from the chamber but it is kept in the aspirating tip during the WBC measurement and the cleaning process.

Sample

S==)@4+d:-3

Micro

2

S=L)@4+'665+6

1

3

1

S=T)/E-6d9D

2 LDL

3

Lyse

Sensor

Macro

Dil M3

LYSE

Lyse

S=K)@4+%3:C96B

Diluent & Cleaner

Sensor

LDD

1

3

2

S=[)@4+$A6B8,B6

S[)%+6:;6B

M1

Hor

M2

Ver

*

Pressure

Meter

Puffer

Reservoir

SL).,99+6

HGB

S=)@B:4;%3:C96B

RBC/WBC

SK)@B:4;$A6B8,B6

Pump

2

3

1

ST)@B:4;*,776B

DILUENT

CLEANER

WASTE


LHL) $-A4B:84;M)84A)N:-34;M)ABJD6--

Both instruments clean the aspirating tip with diluent in the washing head after sampling. It is important to clean the outer surface of the aspirating tip to avoid inaccurate sampling.

The Macro syringe doses and the pump drains the diluent from the washing head, while the aspirating tip moves upwards so the total length of it is washed and cleaned. This process is called total aspirating tip washing.

There is another process, which is washing only a part of the aspirating tip, is the same but the tip does not move in the total length, just a part of it. Some procedures perform this kind of aspirating tip washing.

!"#$%&"'()*/"2 the Macro syringe doses the diluent through V13 (Off), V12 (Off), V11 (On). The

Pump aspirates the diluent from the washing head through V6 (On), while the M2 Vertical motor moves the aspirating tip up.

S==)@4+d:-3

Micro

Dil

2

S=L)@4+'665+6

1

3

LDL

1

S=T)/E-6d9D

2

3

Diluent

Macro

M3

LYSE

Lyse

S=[)*,776B$4B

*

Pressure

Meter xVM

S=K)@4+1+J:8

LDL

WBC

HGB

SU)$-Ad9D

2 1

2 1

S=W)@4+d9D

SV)1+J:8"A

3

3

S`)%+6:;6B

1 2

3

SL)1+J:8@JN;

2 1

3

RBC

M1

Hor

MIX

DILUENT

M2

Ver

CLEANER

Pump

Puffer

Reservoir

ST)@B:4;d9D

S[)*,776B

WASTE

Waste Full Sensor

SK)@B:4;?9D

S=)@B:4;#4e

S_)@B:4;d:-3

!"#$%&"'( the Macro syringe pushes the diluent through V13 (Off), V12 (Off), V11 (On). The Pump aspirates the diluent from the washing head through V4 (Off), while the M2 Vertical motor moves the aspirating tip up.

S==)@4+d:-3

2

S=L)@4+'665+6

1

3

Diluent

1

S=T)/E-6d9D

2

LDL

3

Lyse

Sensor

S=K)@4+%3:C96B

Diluent & Cleaner

Sensor

LDD

1

3

2

S=[)@4+$A6B8,B6

S[)%+6:;6B

M1

Hor

DILUENT

CLEANER

Micro Macro Lyse

M2

Ver

Dil

M3

LYSE

*

Pressure

Meter

SL).,99+6

HGB

RBC/WBC

Puffer

Reservoir S=)@B:4;%3:C96B SK)@B:4;$A6B8,B6

Pump

2

3

1

ST)@B:4;*,776B

WASTE


LHT) @4+,84;M)ABJD6--

The parts of the fluidics are rinsed with diluent. MIX and RBC chambers in !"#$%&"'() */"2 and the measuring chamber in !"#$%&"'( are filled up with 1 ml of diluent. This method prevents the chamber(s) from dirt and makes the diluting process faster.

Q)"(41%3,&'.(3$+?"44()14(143&$1#"!(56( 7,(+*(41%3,"Z(-)&?)(&4(&'(#)"(143&$1#&'.(#&3W(O'(#)"(*&$4#(!&,D#&'.(4#"3(#)" sample is dispensed into the MIX chamber in !"#$%&"'() */"2 and into the measuring chamber in

!"#$%&"'( with 3.0 ml of diluent, which comes from the Macro syringe through V13 (On) and Micro syringe, while the M3 Dilutor motor moves upwards. This process makes the 1:160 first dilution in the chamber.

!"#$%&"'() */"2( #)"( 4"?+'!( 41%3,&'.( 3$+?"44( 143&$1#"4( 56( 7,( +*( 3$&%1$2( !&,D#&+'( 1'!( #)"'( &#( &4 added with 5.0 ml of diluent into the RBC chamber. This process makes the 1:32,000 dilution in the RBC chamber.

Sample + diluent

S==)@4+d:-3

Micro

Dil

2

S=L)@4+'665+6

1

3

LDL

Macro

M3

LYSE

1

S=T)/E-6d9D

2

3

Lyse

S=[)*,776B$4B

*

Pressure

Meter

S=K)@4+1+J:8

LDL

WBC

HGB

3

2 1

S=W)@4+d9D

SU)$-Ad9D

2 1

SV)1+J:8"A

3

S`)%+6:;6B

1 2

3

SL)1+J:8@JN;

2 1

3

RBC xVM

MIX

M1

Hor

M2

Ver

DILUENT

CLEANER

Puffer

Reservoir

ST)@B:4;d9D

S[)*,776B SK)@B:4;?9D

S=)@B:4;#4e

S_)@B:4;d:-3

Pump

WASTE

Waste Full Sensor

! In stored in the aspirating tip during the WBC measurement and the cleaning process and the instrument makes the second dilution into the chamber after these processes.

Sample + diluent

S==)@4+d:-3

2

S=L)@4+'665+6

1

3

1

S=T)/E-6d9D

2

LDL

3

Lyse

Sensor

S=K)@4+%3:C96B

Diluent & Cleaner

Sensor

1

LDD

3

2

S=[)@4+$A6B8,B6

S[)%+6:;6B

M1

Hor

DILUENT

CLEANER

Lyse

M2

Ver

Micro Macro

Dil M3

LYSE

*

Pressure

Meter

SL).,99+6

HGB

RBC/WBC

Puffer

Reservoir

S=)@B:4;%3:C96B SK)@B:4;$A6B8,B6

Pump

2

3

1

ST)@B:4;*,776B

WASTE


LH[) /E-4;M)ABJD6--

! In

(On) and V4 (On) into the WBC chamber), because the vacuum – which is generated by the pump – aspirates the fluid through V8 (On), while V3 and V7 are Off. Simultaneously, 0.7 ml of lysing reagent is added through V14 (On), while the Lyse syringe moves upwards. This process makes the 1:190 dilution rate and good mixing with lyse, and at the end the mixing chamber is empty, and the specified RBC and WBC dilutions are in the proper chambers. After this step the system makes a MIX chamber washing cycle.

S==)@4+d:-3

Micro

2

S=L)@4+'665+6

1

3

LDL

1

S=T)/E-6d9D

2

3

Lyse

Macro

Dil M3

LYSE

Lyse

S=[)*,776B$4B

*

Pressure

Meter

S=K)@4+1+J:8

LDL

WBC

HGB

SU)$-Ad9D

2 1

3

3

2 1

S=W)@4+d9D

SV)1+J:8"A

S`)%+6:;6B

1 2

3

SL)1+J:8@JN;

2 1

3

RBC xVM

M1

Hor

MIX

DILUENT

M2

Ver

CLEANER

Pump

Puffer

Reservoir

ST)@B:4;d9D

S[)*,776B

WASTE

Waste Full Sensor

SK)@B:4;?9D

S=)@B:4;#4e

S_)@B:4;d:-3

! In

(On), while the Lyse syringe moves upwards. This process makes the 1:190 (WBC) dilution rate and good mixing with lyse (the syringe also pushes some air bubbles for mixing).

S==)@4+d:-3

Micro

2

S=L)@4+'665+6

1

3

1

S=T)/E-6d9D

2

LDL

3

Lyse

Sensor

S=K)@4+%3:C96B

Lyse

Diluent & Cleaner

Sensor

1

LDD

3

2

S=[)@4+$A6B8,B6

S[)%+6:;6B

M1

Hor

Macro Lyse

Dil M3

LYSE

*

Pressure

Meter

SL).,99+6

HGB

RBC/WBC

Puffer

Reservoir S=)@B:4;%3:C96B SK)@B:4;$A6B8,B6

M2

Ver

DILUENT

CLEANER

Pump

2

3

1

ST)@B:4;*,776B

WASTE


LH_) d.%)DJ,;84;M)ABJD6--

! In diluent reagent coming from the macro syringe. V12 & V7 are On, V10, V9 and V3 are OFF, and pump drains the reagent. By this time the pump has generated the regulated vacuum in the puffer reservoir.

After that, the vacuum aspirates the diluted sample from the WBC and RBC chambers through V3 (ON) and

V5 (ON) valves while all others are OFF (during this the pump is OFF which is equivalent to a closed valve).

Both WBC and RBC samples are flowing through the aperture but the float measures only the WBC sample volume by moving down until the lower opto switch is off. When the float reaches the lower opto, V3 is closed. In this state the HGB measurement is executed.

S==)@4+d:-3

Micro

Dil

2

S=L)@4+'665+6

1

3

LDL

Macro

M3

LYSE

1

S=T)/E-6d9D

2

3

Lyse

S=[)*,776B$4B

*

Pressure

Meter

S=K)@4+1+J:8

LDL

WBC

HGB

3

2 1

S=W)@4+d9D

SU)$-Ad9D

2 1

SV)1+J:8"A

3

S`)%+6:;6B

1 2

3

SL)1+J:8@JN;

2 1

3

RBC xVM

M1

Hor

MIX

M2

Ver

DILUENT

CLEANER

Puffer

Reservoir

ST)@B:4;d9D

S[)*,776B SK)@B:4;?9D

S=)@B:4;#4e

S_)@B:4;d:-3

Pump

WASTE

Waste Full Sensor

!"#$%&"'( the regulated vacuum (it is generated by the pump in the puffer reservoir) aspirates the diluted sample (WBC) from the chamber through V2 (On) valve. There is no volume limiter, so the instrument counts the cells for 8 seconds.

At the end of this process the HGB measurement is executed.

S==)@4+d:-3

2

S=L)@4+'665+6

1

3

1

S=T)/E-6d9D

2

LDL

3

Lyse

Sensor

S=K)@4+%3:C96B

Diluent & Cleaner

Sensor

1

LDD

3

2

S=[)@4+$A6B8,B6

S[)%+6:;6B

M1

Hor

DILUENT

CLEANER

Micro Macro Lyse

M2

Ver

Dil M3

LYSE

*

Pressure

Meter

SL).,99+6

HGB

RBC/WBC

Puffer

Reservoir

S=)@B:4;%3:C96B SK)@B:4;$A6B8,B6

Pump

2

3

1

ST)@B:4;*,776B

WASTE


LHV) ?.%)DJ,;84;M)ABJD6--

! In when the float is at the lower opto sensors. The measuring vacuum (which is in the puffer reservoir) aspirates the RBC sample through V7 (ON) and V5 (ON) valves while the V3 valve is OFF. Both, the WBC and the RBC samples are flowing but the float measures only the RBC sample volume by moving upwards.

[&#)(#)&4(%"#)+!(1(3$"!"*&'"!(41%3,"(N+,D%"(E5\\( 7,G(&4(%"14D$"!Z(8D#()"([<A(1'!(#)"(P<A(%"14D$&'.

times are different because the diameter of the cell-counter apertures are different.

S==)@4+d:-3

Micro

2

S=L)@4+'665+6

1

3

LDL

Macro

Dil M3

LYSE

1

S=T)/E-6d9D

2

3

Lyse

S=[)*,776B$4B

*

Pressure

Meter

S=K)@4+1+J:8

LDL

WBC

HGB

SU)$-Ad9D

2 1

3

2 1

S=W)@4+d9D

SV)1+J:8"A

3 S`)%+6:;6B

1 2

3

SL)1+J:8@JN;

2 1

3

RBC xVM

M1

Hor

MIX

M2

Ver

DILUENT

CLEANER

Pump

Puffer

Reservoir

ST)@B:4;d9D

S[)*,776B

WASTE

Waste Full Sensor

SK)@B:4;?9D

S=)@B:4;#4e

S_)@B:4;d:-3

!"#$%&"'( the RBC counting process is absolutely the same as the WBC from the pneumatical point, but the diluted sample – which is aspirated by the regulated vacuum from the chamber – is the second dilution rate made by the second dilution process. RBC counting time is also 8 seconds.

LHU) d:-34;M)ABJD6--6-

Both instruments make the necessary washing processes such as MIX, WBC and RBC chambers washing processes in !"#$%&"'()*/"2, and chamber washing process in the !"#$%&"'(.

The systems has a two-step washing method, which ensures a very low carry over between measurements. At the first step – called pre-washing – 1 ml of diluent washes the actual chamber and the pump drains it. At the next step – called main washing – the actual chamber is primed with diluent. This diluent volume is higher (+ 0,2 ml) than the chamber’s operating sample volume.

Sometimes (for example the MIX washing in !"#$%&"'() */"2) this second diluent is not drained immediately after the washing process, it stays in the chamber protecting it from contamination. In this case the chamber is drained at the end of the total washing process.

Draining is a very simple fluidic method. The Pump aspirates the fluid from the actual chamber or tube through its draining valve is On while the other valves are Off.

In !"#$%&"'( the washing process also use Cleaner solution to clean the chamber from the lyse reagent between the WBC and RBC measurements and after each measurement cycle.

High-voltage burning pulses are applied to the apertures in each measurement cycle to prevent lipid and protein build-up.


LH`) %+6:;4;M)ABJD6--

! In cleaner reagent. The pump aspirates the cleaner through the V7 (On), V3 (Off) and V9 (On) valves.

After that the instrument cleans the WBC chamber with cleaner reagent (Section 6.4).

The instrument generates vacuum in the WBC chamber (with the pump), and after that this vacuum aspirates the cleaner reagent into the WBC chamber through V9 (On), V3 (Off), V7 (On), V8 (On). This second process is called $,8JD+6:;)(see the figure), which is a part of the normal cleaning cycle.

S==)@4+d:-3

Micro

2

S=L)@4+'665+6

1

3

LDL

Macro

Dil M3

LYSE

1

S=T)/E-6d9D

2

3

Lyse

S=[)*,776B$4B

*

Pressure

Meter

LDL

WBC

HGB

S=K)@4+1+J:8

3

2 1

S=W)@4+d9D

SU)$-Ad9D

2 1

SV)1+J:8"A

3

S`)%+6:;6B

1 2

3

SL)1+J:8@JN;

2 1

3

RBC xV

M

M1

Hor

MIX

Cleaner

DILUENT

M2

Ver

CLEANER

Puffer

Reservoir

ST)@B:4;d9D

S[)*,776B SK)@B:4;?9D

S=)@B:4;#4e

S_)@B:4;d:-3

Pump

WASTE

Waste Full Sensor

!"#$%&"'( the pump aspirates the cleaner through the V5 (On), V15 (On), V2 (On) and V4 (On) valves to clean the aperture. After that the instrument cleans the chamber with cleaner reagent that remained in the tubes between V12, V5 and the liquid detector (Dil. And Cleaner detector): the Macro syringe doses diluent and cleaner reagents (from the tubes) through V13 (Off), V12 (On), V15 (Off) and V3

(On) into the chamber.

S==)@4+d:-3

Micro

2

S=L)@4+'665+6

1

3

1

S=T)/E-6d9D

2 LDL

3

Lyse

Sensor

Macro

Dil M3

LYSE

Lyse

S=K)@4+%3:C96B

Diluent & Cleaner

Sensor

1

LDD

3

2

S=[)@4+$A6B8,B6

S[)%+6:;6B

M1

Hor

M2

Ver

*

Pressure

Meter

Puffer

Reservoir

SL).,99+6

HGB

S=)@B:4;%3:C96B

RBC/WBC

SK)@B:4;$A6B8,B6

Pump

2

3

1

ST)@B:4;*,776B

DILUENT

CLEANER

WASTE


T) $@i"2(#0'(

There are some very important mechanical and hardware settings in both (!"#$%&"'() */"2 and

!"#$%&"'() systems and there are some software settings, which are available only for Service Personnel.

The mechanical and hardware adjustments are described in this section. Special software settings are included in the 5.2 section of this Service Manual.

TH=) #6D3:;4D:+)-6884;M-

There are two very important and necessary mechanical settings in both instruments:

! &A8J)N366+)-6884;M

! $-A4B:84;M)84A)-6884;M

>88')2)8GT%"&')"%')*b0&3%*'IG'3E%'1)207)$30"%"'I%7#"%'&E5D1%23H'I03'3E%"%'5&')'D#&&5I5853G'3#

$E)2F%')2G'D)"3'#7'3E%'1%$E)25$)8'&G&3%1')2*'52'3E5&'$)&%'3E%'A%"O5$%'!%"&#22%8'10&3'*#

3E%&%'51D#"3)23'&%3352F&K'?E%'#15&&5#2'#7'3E%&%'&%3352F&'$)2'$)0&%'1)8702$35#2'#"'*)1)F&

3#'3E%'52&3"01%23K

TH=H=) &A8J)N366+)-6884;M

This setting may be necessary for the vertical motor movements because this adjustment sets the opto detectors of the H&V moving unit. The top of this block is called HV head and it is shown in the figure below.

$ Set the distance to KPL)CC between the two parts of the head.

„A” screws

2-3 mm

$ Loose the „A” screw for free movement on the timing belt.

$ Adjust the opto wheel to the home position while the head stays untouched. Fix the „A” screws.

„B” screws

Home position is reached when the HOME hole is in the HOME opto sensor, and the HOME state LED goes

OFF on the H&V motor opto board (see picture below).

After that check the END position, as well: move the needle to the lowest position (it is indicated by END state

LED). In that state =PK)CC distance must be between the carriage and the bottom of the H&V moving unit.

HOME state LED

END state LED

END opto sensor

HOME opto sensor

OPTO wheel

END hole

HOME hole


TH=HK) $-A4B:84;M)84A)-6884;M

This adjustment sets the aspirating tip to the operational position. Set the distance between the two parts of the

HV head to 5 mm. Loose the „B” screws and adjust the aspirating tip to its operational position.

Bottom plane of washing head

Aspirating tip

Teflon sealing ring

Tubes to/from the washing head

Washing head is located in the bottom of the H&V moving unit, which is shown in the figure above. Use the

Needle setting menu point in Service/Miscellaneous menu. The instrument will move the motors to setting position, and hold the carriage, so that adjustment can be made safely, without unintentional moving of the carriage.

A%3' 3E%' D#523' #7' 3E%' 35D' 3#' 3E%' N)&E52F' E%)*c&' I#33#1' D8)2%H' NE58%' 3E%' $)""5)F%' 5&' E%8*' IG

1#3#"&K''+:%%*8%'&%3352F'1%20/K';5V'3E%'d(\'&$"%N&K

After setting, exit from the Needle setting menu and check the position of the tip and if it is necessary make the adjustment again.

THK) !:B5N:B6)-6884;M-

The most part of the hardware settings are adjusted by the manufacturer and it is not required to reset these settings. There is only one part in both instruments, which claims some adjustment; this is the sensitivity of the liquid detectors.

THKH=) $5Y,-8C6;8)J7)836)+4b,45)5686D8JB)-6;-484G48E

Liquid/reagent detector sensitivity may be necessary to be adjusted.

Follow the procedure below to find the optimal setting:

1. Without any reagents in the sensor tube, set the Diluent/Cleaner and Lyse potentiometers in the PCPRO board to reach the state, when the LED goes off.

2. After that turn the potentiometers counterclockwise to reach the on state (the LED is on) and after that turn 180° more in the same direction.

3. Check that LEDs go off, when priming with reagents.

To check the proper operation of the liquid detectors, use the indicator LEDs:

No reagent

Reagent, with bubbles

Reagent, without bubbles

LED is on

LED sometimes turns on

LED is off


THKHK) $CA+4746B)J77-68)-6884;M

Amplifier offset should be between ±5mV. Run self test to determine whether offset is within this range. If it is out of range, it should be re-set, by the following way.

$ In Maintenance/Service/Password/Miscellaneous menu press Offset adjustment menu.

$ Adjust the potentiometer to reach 0 mV.

Offset adjustment for !"#$%&"'() */"2 is not so easy, the potentiometer can be reached from the internal space only.

!"#$%&"'( offers easier access for the amplifier offset setting element. It is located on the measuring block.


[) %!0%a>'Q)&1)*?&*0?)&*0?$(>&'

Both analyzers have built-in Self test and Service menu.

[H=) 26+7)86-8

[H=H=) 26+7)(6-8)2DB66;-

Self test can be used to check the operation of the instrument.

Self test results

The first panel shows general information about the instrument.

(See menu on the left.)

Date of testing:

Model:

21.05.2004

HUMACOUNT PLUS

Serial No:

Version:

Compiled: 21.05.2004

PCPNIF Firmware version

MPU Firmware version:

2541

2.9

---

2.7

The PCPNIF firmware version will always be empty “- - -“, the

MPU firmware version holds the MPNIF version number.

Self test results

HGB dark:

HGB light

Electr. Voltage:

current:

offset

Ampl. test:

peak:

dev:

275

17723

51.2 V

879 uA

-1.9 mV

19999 pls

1641 mV

53 mV

Self test results

Noise test:

Atm

Vacuum:

drift:

1 pls/5 sec OK

978 mBar

218 mBar

OK

OK

3 mBar/10sec OK

Overall result: Successful

OK

OK

OK

OK

OK

OK

OK

OK

The second and third panel contains tested parameters, as follow:

$ !Q.) 5:B< count (HGB LED is off, this parameter is always zero in the !"#$%&"'(), and +4M38 (LED is on).

$ 0+6D8BJ56) GJ+8:M6, measuring D,BB6;8 and amplifier

J77-68.

$ $CA+4746B by generating 20000 86-8 pulses, including gain related A6:< value, noise related 56Giation.

$ $8CJ-A36B4D)AB6--,B6 stands for outer pressure.

$ S:D,,C reports pump operation (vacuum generated by the pump in a 10-second period of time).

$ @B478 represents pressure loss of vacuum measured in a

10-second period of time.

At the end of each result line a -8:8,-)C6--:M6 is displayed, which means that the actual test result is in the normal range (GC), higher (EHDE), or lower (9GI) than the pre-determined limits, or the result produced an error (1JJGJ).

&G6B:++)86-8 result is displayed, which can be F+332;;=+*#(in case of every test result is GC) or 1&&'&;#(if any of the parameters is EHDEK#9GI or 1JJGJ).


[H=HK) 'JBC:+)B:;M6)J7)26+7)86-8)A:B:C686B-

*:B:C686B

HGB dark

HGB light

Electrode voltage current offset

Amplifier test peak of pulses deviation (noise)

Noise test

Outer pressure

Vacuum

Drift

[H=HL) (BJ,9+6-3JJ84;M)Q,456)7JB)26+7)86-8

";48 count count

V

µA

MV count

MV

MV pls/5sec mBar mBar mBar/10sec

*:B:C686B

HGB dark

HGB light

Electrode voltage

Current

Offset

Amplifier test

Peak of pulses

Dev. (noise)

Noise

Outer pressure

Vacuum

Drift

#:B<

EHDE

9GI

EHDE

9GI or#EHDE

9GI or#EHDE

9GI or#EHDE

9GI

EHDE

9GI or#EHDE

EHDE

EHDE

9GI or#EHDE

9GI

EHDE

/JN6B)9J,;5

0

3000

45

830

-5.0

19990

1300

0

0

600

125

0

"AA6B)9J,;5

3000

50000

55

930

5.0

20050

1700

100

50

1100

250

10

*J--49+6)B6:-J;

Instrument door open

HGB head not connected or

HGB LED out of order

Instrument door open or

HGB LED too bright

Fault on High Voltage or Amplifier board

?6C65E

Close instrument door

Check HGB head connections or check HGB LED operation during measurement

Close door or replace HGB head

Check measuring voltage (50V) on

High voltage and Amplifier boards

Fault on Amplifier board

Check current generator, relays and test generator FET on

Amplifier board

Unadjusted Offset on

Amplifier board

MPNIF and Amplifier Boards are not connected

Instrument not grounded

Fault on Amplifier board



Pressure sensor, cable, amplifier or PIC#0 error

Peristaltic pump failure

Leakage in pneumatics

Adjust the offset potentiometer on

Amplifier board

Check cables and connectors between MPNIF and Amplifier

Check mains ground lead

Check current generator, relays and test generator FET on

Amplifier board



Check pressure sensor on PRO board, cable connections and

MPNIF board pressure amplifier

Check peristaltic pump & tube

Check tubing in pneumatics


[HK) 26BG4D6)#6;,

[HKH=) 0;86B4;M)8J)26BG4D6)#6;,

There is a Service menu for servicing and operation checking purposes. The entry point is in the

Maintenance/Service screen, where Service Information is displayed.

]23%"'3E%'$#*%'3#')$$%&&'A%"O5$%'1%209'eYfY

[HKHK) #:4;)26BG4D6)#6;,

The Main Service menu provides access to submenus and service utilities.

Service menu

1. Edit Service contact

2. Device information

3. Calibration

4. Settings

5. Testing

6. Miscellaneous

[HKHL) 0548)26BG4D6)%J;8:D8

Service information

Your service partner:

Your Distributor

Address:

Phone:

Contact Person:

The information set up here is shown in the Maintenance/Service screen for the user.

You can edit the Information fields by cursor keys, or by an external keyboard.

Press the OK button, if a field is completed.

[HKHT) @6G4D6)>;7JBC:84J;

Device information

Model:

Serial No:

Version:

Compiled:

HUMACOUNT PLUS

1359

2.3

21.12.2001.

In the Device Information menu the model name, the serial number, the software version and compilation date appear.

[HKH[) 26BG4D6)%:+49B:84J;

Calibration menu is provided for Service calibration purposes.

Calibration

1. Calibration factors

2. Apply user factors

In result calculations the service calibration factors are used as the user calibration factors, so they are multiplied for each parameter:

?.%

@4-AH

)j)1:D8

?.%)"-6B

)c)1:D8

?.%)26BGH

)c)?.%

#6:-,B65

If the user factor is near the bound (0.80 – 1.20), by setting the corresponding service factor, the user factor can be adjusted to

1.00.


1:D8

?.%)"-6B

)= 1.19 and)1:D8

?.%)26BG)

= 1.00, result for RBC.

:;5 1:D8

?.%)"-6B

)= 1.00 and)1:D8

?.%)26BG)

= 1.19 gives the same

$AA+E) ,-6B 7:D8JB- function can be used to move the combination of the existing user and service factors, automatically to the Service level. If all service calibration factors were 1.00, after this function all service factors will be set to the product of the previous user and service factors, and all user factors will become 1.00.

[HKH_) 2J78N:B6)26884;M-

Software settings can be customized in this menu.

Settings

Autoclean cycle (0..50):

Standby time (10..60 min):

Reblank time (0..8 hours):

Rinse Time (0..3 hours):

Serial I/O speed (Baud)

Serial Protocol Version

Waste container capacity (1..60 l)

Laboratory header lines:

2 0

15

1

1

Offline

2.20

18

4

$,8JD+6:;) DED+6) ]!"#$%&"'() */"2) J;+E^: after how many measuring cycles HUMACOUNT PLUS makes an autoclean process (default = 20 cycles). Zero means ;6G6B!

Lower this setting if periodic noise appears in the system, i.e. 12-

15 measurements good, and after that 5-8 measurements with high PLT.

28:;9E) 84C6: after how many minutes the instrument goes to standby mode. This is the setting used in Measure mode, if Start has not been pressed, chambers will be primed to avoid clogging of aperture (default = 15).

?69+:;<) 84C6: after how many hours the instrument makes a blank measurement if it was in stanby mode

(default = 1 hour). If it is set to 0, then no ReBlank is performed.

?4;-6)84C6: If this time is set, then the instrument will make an automatic, extended washing procedure (with more diluent) of the chamber(s) to get wash dirt out of the chambers (dust). If it is set to 0, then no Rinse is performed.

26B4:+)>F&)-A665: instrument can send results to a host computer via serial port with the RS232 V24 protocol.

The baud rate of this serial communication must be selected at this point. For details of the serial communication protocol, see Appendix 9.2 in this manual.

This setting must be set to the desired baud rate, otherwise the link to PC is not possible.

26B4:+)*BJ8JDJ+)S6B-4J;: with this option, the used protocol (sending specific fields or not) can be selected. This is useful when a new software (with more stored e.g. patient info) is installed in laboratory system, where an earlier serial protocol has been implemented.

d:-86) DJ;8:4;6B) D:A:D48E) ]!"#$%&"'() J;+E^: to set the correct volume of the waste container for proper usage of the software ”waste full” alert. Set this value 2litres less than the total volume of waste container.

/:9JB:8JBE) !6:56B)/4;6-: this setting controls the available lines to set up the laboratory header on printed reports. The header text can be edited on user level.

Settings

Disable multiuser mode:

Disable 3 part diff:

Disable markers

Stretch histograms

Language

Native keyboard

Maintenance day

Patient data

English

Thursday

Birthdate

No

No

No

No

No

@4-:9+6) C,+84P,-6B) CJ56I by this setting the multi-user mode can be disabled on the user level

@4-:9+6) LPA:B8) 5477HI) for using quick lyse (without 3-part differential) the errors and the bad 3-part parameters can be excluded from the results

@4-:9+6) C:B<6B-I setting this option to Yes will omit the markers from the reports (it is commonly used for Veterinary application)

28B68D3) 34-8JMB:CI setting this option to Yes will modify the

WBC histogram from 0-300 fl instead of 0-400 fl (this is recommended for Veterinary mode)

/:;M,:M6I this setting will adjust the user language. The Service menu always in.

L$-!*-<*2# *-",+-,2;M# L&-<!3K# 1",*!;@K# 4&2"3@K# D2&8-"K# D&226K# E+",-&!-"K# H".'"2;!-"K# H%-*!-"K# )'*!;@K# )'&%+,+2;2K# J'8-"!-"K# J+;;!-"K

FN-"!;@K#A+&6!;@#-".#O!2%"-82;2P#:@!"2;2#*-",+-,2#!;# available #'"*/#='&#;N23!-*#8'.2*;P

':84G6)<6E9J:B5I Setting it to Yes will utilize the external PC keyboard with the language specific layout.

#:4;86;:;D6)5:EI You can select among the days of the week. According to this setting, the instrument will warn the user to do the weekly maintenance when shut down.


*:846;8) 5:8:I There are two options: 5!&%@.-%2 and L,2. According to this setting, in the sample information dialog and in the database, the instrument will prompt for the age or the date of birth. If age is specified, it can be given in years or months.

Settings

*B654+,84J;) B:84JI) can be selected between 1:10 or 1:3. This ratio is used in Pre-dilution mode.

Predilution Ratio 1:3

Reagent code

Extend ProbeVoltages:

Calendar Mode

LCD-light off delay (hrs): 0

9

Yes

Gregorian

?6:M6;8)DJ56I a value)to modify the zero point of HGB function, valid between 0-30, (default =9). For pre-diluted sample, HGB can have a significant offset due to reagent non-linearity.

Set code = 0, and measure HGB in pre-diluted mode, and in normal mode. Adjust the code until the 2 HGB results are the same, then calibrate HGB. Use this reagent code with your reagents.

0e86;5)*BJ96)SJ+8:M6-I This option is ON by default. It makes the instrument accept the physically highest probe voltages: 450 on both channels (it is expressed in A/D resolution, where 1023 shows 55V, so 450 is practically 24.2 V on the electrode).

Probe Voltage – displayed in the measurement results screen are representing the electrical probe potential of the measuring electrode. They are displayed in a range, like

RBC Probe Voltage: RPrV = 223-228,

WBC Probe Voltage: WPrV = 245-249

Probe voltage can change a maximum of ± 4%. If it changes more than 4%, or it is higher than 420 (or 450 if

Extend Probe Voltages in On), the analyzer will display an error, and ‘C’ or ‘c’ flag will show clogging condition on the corresponding channel.

%:+6;5:B) CJ56I You can select between the Gregorian and the Jalaali (Persian) calendar. All dates will be converted accordingly.

/%@) +4M38) J77) 56+:EI The time that elapses without any user activity to turn off high-voltage You can select between the Gregorian and the Jalaali (Persian) calendar. All dates will be converted accordingly.

[HKHV) 26BG4D6)(6-84;M)#6;,

Service Testing menu provides tools for checking operation, fluidics and hardware.

Testing

1. Self test

2. Valve test

3. Display test

4. Stress

5. Cleaning (5 cycles)

From this submenu 26+7)86-8 can be run (see Section 5.1).

[HKHU) S:+G6)(6-8)#6;,

In Valve Test menu valve numbers and their state are displayed.

The number under each valve represents the actual state of the valve. Selected valve number is shown in inverse.

0 is &77 state, 1 is &; state. S:+G6)=_)C6:;-)A6B4-8:+84D)A,CA.

!"#$%&"'( has no valves 6-10.

Use cursor keys for selecting and setting valve state, or press

OK to toggle state.


[HKH`) @4-A+:E):;5)a6E9J:B5)(6-8

Display and keyboard test is provided to check keypad and LCD panel.

Press a key to test and the LCD will show the code of the pressed key, and will be invert the colors at each touch of a key for testing that every LCD dot is functioning properly.

[HKH=W) 28B6--)CJ56

In 28B6--)CJ56R the instrument performs measuring cycles without sample (blank measurements) continuously.

This can be used for burn-in tests, or to check/clean pneumatic system after changing any main fluidic parts.

You can have information about stability, cleanliness, HGB operation, and for HUMACOUNT PLUS the float operation. Results of the last 10 PLT and HGB blank is displayed as well.

You can detect any kind of noise, or bubbles in the system if the PLT is not stable low, or HGB has big variation.

The operation of the volume limiter tube can be monitored by the displayed floating (measuring) time for RBC and WBC measurement, and besides their calculated minimum, maximum and the standard deviation.

To exit from this mode AB6--)2($?()9,88J; (at the end of a normal cycle) until the Stress operation is finished.

[HKH==) #4-D6++:;6J,-)-6884;M-

Miscellaneous

1. Software upgrade

2. Clear device statisctics

3. Clear database

4. Offset adjustment

5. Needle setting

6. Log in as supervisor

In this menu, you can directly perform 2J78N:B6) ,AMB:56 (this will restart the instrument).

%+6:B) @6G4D6) 28:84-84D-:) Device statistics (number of measurements, aperture-clogging and other errors) can be cleared.

%+6:B) @:8:9:-6: The whole Database (measurement results including histograms) can be cleared.

&77-68)$5Y,-8C6;8: Provides an easy way to set the offset value (see Section 4.2.2).

$ Enter this menu to check and/or adjust the offset voltage of the amplifier.

$ Adjust the potentiometer to reach 0 mV.

'665+6)-6884;M: This submenu provides an easy way to set the needle position (see Section 4.1.2).

You can /JM)4;):-)-,A6BG4-JB if the Multi user mode was selected in the User’s Settings submenu. (Number 6.

- if present, shows the availability of this function.)

In supervisor mode, you have the ability to change any user passwords and you have full access over user settings.)$+N:E-)+JM)J,8):786B)834-)-,A6BG4-JB)+JM)4;H

[HKH=K) )?6-D,6)%J56)7JB)#,+84P,-6B)#J56

It the supervisor password has been forgotten, there is a rescue code for service purposes to access the supervisor level. Rescue data are:

User ID:

Name:

Password:

W

26BG4D6R

(zero),

VK`T[_)(This forms a capital „A” on the keypad)


_) 20?S>%0)&*0?$(>&'2

_H=) &A6;4;M)J7)836)4;-8B,C6;8

The left side of both instruments is a side door, which allows to reach the fluidic system easily. Other parts of the analyzers can be serviced by opening the cover.

! !"#$%&"'() */"2: first unscrew the 3 cover fixing (“Z”) screws located on the rear panel. After this, open the left side door. In the front part of the analyzer there is a special zipfastener as it is shown in the figure.

Push this fastener to the direction of the arrow this will unlock the fastener and after that the cover is ready to open. Pull the cover upward to open the instrument.

To lock the cover, pull the fastener to the opposite direction.

rear panel the cover is ready to open. But this instrument has a special sliding rack system for the main board holder plate to provide easier access for cables:

By loosening the main board holder plate fixing screws on the rear panel the Service

Personnel can pull out the entire main board holder plate with the main board from the front of the instrument and access the back of the assembly plate.

Figure on the left shows a dismounted

!"#$%&"'( for easier understanding.


_HK) ?6CJG4;MF>;-8:++4;M)@4CC*%

_HKH=) ?6CJG4;M)@4CC*%

The DIMM-PC can be dismounted in the following way (see Figure below):

1. Discharge all electricity from your body by touching the instrument case before proceeding. DimmPC is very sensitive against electrostatic discharge.

2. Remove the safe lock screw (M2 x 10 mm, hexagonal).

3. Open the two white levers on both sides so that Dimm-PC flips up.

4. Pull out DimmPC module from the socket in a sloping angle.

2. Remove screw

3. Open levers

C5&1#02352F'#7'C511!,g

_HKHK) >;-8:++4;M)@4CC*%

1. Discharge all electricity from your body by touching the instrument case before proceeding. DimmPC is very sensitive against electrostatic discharge.

2. Place the DIMM-PC with edge connector to the socket with Heatsink up, at approx. 30° angle and push it softly into the socket. Do not to force it because the socket can be damaged. Pull apart the white holder on both sizes and snap the module into place by pushing it down.

3. Make sure the levers are back in place keeping the module down.

4. Screw in the safe lock screw with the plastic ring (M2 x 10 mm, hexagonal).

>7!88):#!;#%@2#%&-.2#8-&6#'=#C'"%&'"#18<2..2.#B'.+*2;#D8<EP

_HL) #@$)]#J;JD3BJC6)@4-A+:E)$5:A86B^)6C,+:84J;)CJ56

MDA emulation mode was developed to provide video output to be displayed on the LCD.

This mode is available during the total operation but especially useful for checking the BIOS setup and OS functions. This mode should be handled the same as the service menu, the user does not have to know these options.

At power-on, the controller PIC on MPNIF card is configuring the FPGA chip and displays the greeting screen.

After 6 seconds, it starts to scan the keypad and behaves like an #@$ adapter to the PC, although the displayed screen is still holding the greeting screen.

If you switch to #@$) 6C,+:84J;) CJ56, the system boot events can be displayed, and the CMOS SETUP program can be started, if necessary.

If the main program does not start in 90 seconds, the PIC will automatically switch to MDA displaying mode to give information about the reason of hang-up. This can be caused by some CMOS failure, e.g. there is a nonbootable floppy disk in the drive, and boot order is set to start on floppy disk, and the system is waiting for user confirmation: “Remove disk, and press a key”.

If you need to run BIOS setup, an external keyboard must be connected.

Hold the „k” button, and press and release the „H” button on the foil keypad of the analyzer to activate the #@$ mode. (Use the “?” like a shift button). The display will use the LCD like a primary computer monitor (80 characters by 25 rows). The LCD is smaller than the standard monitor area therefore just about a quarter of the total screen is displayed at once (40 characters by 16 rows).


$ to change the actual quarter displayed, use the cursor keys: `(a(b(c(+'(#)"(U"231!Z

$ to go where the cursor is (cursor follow operation), press the „k” button,

$ to go back to the normal graphic displaying mode press „kl):;5)m)H” again.

Quarter 1 on LCD Quarter 2 on LCD

Quarter 3 on LCD Quarter 4 on LCD

C%752535#2'#7'*5&D8)G%*'@,C'h0)"3%"&'52'.C>'%108)35#2'1#*%

The MDA screen and the Graphical Application Display are existing in the same time. They use different area of the video RAM located on the MPNIF board.

Displaying is controlled by the configuration PIC on the MPNIF board, and hosted by the FPGA. If there is no display in MDA mode (or garbage characters are displayed), check the following points:

1. MPNIF board for correct connection via the ISA slot

2. DimmPC connector, the CPU is not placed correctly in the socket or there is bad contact

3. Replace the DimmPC

_HT) a6E).>&2)-6884;M-)7JB)DJBB6D8)JA6B:84J;

To start the setup utility, keep repeatedly pressing the <F2> key on the external keyboard during startup. The start of the Setup program is indicated by the keyboard status LED’s coming on and off, then only NumLock remains on. Additional beeps (when <F2> is pressed) indicate running of the Setup program.

Now, use the k and H)]5J8^ keys on the instrument keypad to enter MDA mode, to see computer display on LCD.

With MDA mode, you get the normal PC screen displayed on the LCD. As the LCD is smaller than the screen, only a part (one quarter) of the screen is shown. You can move this display window of the screen using the arrows on the keypad. If you press k, the displayed quarter will follow the cursor.

(J)-6+6D8):;)486C, simply use the arrow key to move the cursor to the field you want. Then use the plus and minus keys to select a value for that field. The Save Value commands in the Exit Menu save the values currently displayed in all the menus.

(J)54-A+:E):)-,9)C6;,, use the arrow keys to move the cursor to the sub menu you want. Then press <Enter>.

A pointer marks all sub menus.

_HTH=) .>&2)26884;M-)J7)@4CC*%

Main menu

Legacy Diskette

Primary Master

Primary Slave

Advanced Menu

Reset Configuration Data

Halt On Errors

CPU Speed

ISA bus cycle duration:

I/O Device Configuration submenu:

Local Bus IDE Adapter

Floppy disk controller

Base I/O address

Serial port A

Serial port B

Parallel Port

$"(&

=HTTF=HK[)#.R)L)nl

$"(&

UWW);-

'J

'J

=LL)#!O

0;:9+65

0;:9+65

*B4C:BE

L1U3R)>?oT

K1U3R)>?oL

0;:9+65R)0**R)LVU3


Setup Boot Menu

Floppy Check

Summary Screen

QuickBoot Mode

Dark Boot

Boot Device Priority submenu:

+ Hard Drive

Add-on cards

SanDisk

Primary Slave

+ Removable Devices

Legacy floppy drives

CD ROM

Network Boot

@4-:9+65

@4-:9+65

0;:9+65

@4-:9+65

_HTHK) 0e48)#6;,

Exit Saving Changes

Saves all the selections and exits setup. The next time you boot, the BIOS configures the system according to the Setup selection stored in CMOS.

Exit Discarding Changes

Use this option to exit Setup without storing in CMOS any new selections you may have made. The selections previously in effect remain in effect.

Load Setup Defaults

Select to display the default values for all the Setup menus.

Discard Changes

If, during a Setup session, you change your mind about changes you have made and have not yet saved the values to CMOS, you can restore the values you previously saved to CMOS.

Save Changes

Saves all the selection without exiting Setup. You can return to the other menus if you want to review and change your selection.

Would you make a mistake, or get lost among settings, you can restore defaults anytime, by selecting “Load

Setup Defaults” and starting all over again.

_H[) @&2)1,;D84J;-)J;)836)>;-8B,C6;8

To check the main board as a PC motherboard, or for other service actions, you may wish to run the instrument like a normal PC.

In this case an #2P@&2 6.22 operating system 9JJ8)7+JAAE disk is required.

Follow the steps below to boot from an MS-DOS diskette:

! Connect an external PS/2 keyboard

! After turning on, immediately press repeatedly F2 on the external keyboard, until you see the keyboard lights flashing on (or you hear beeps)

! Switch to MDA mode by mkl)and)mHl, and press `(1'!(a(?D$4+$(U"24(+'(#)"(U"231!(#+(4#18&,&^"(4?$""'

! Use arrows on the keyboard to go to the BOOT section of the menu, and go down to 5''%#.2$!32#N&!'&!%/ menu, press Enter

! The list shows the boot order, move the cursor to the item you wish to change, then press +/- on the keyboard to move it higher/lower in the list. Move J28'$-<*2#.2$!32; first.

! Press Esc key until you get to the Exit menu, and press Save changes, then Enter to confirm

! Put the MS-DOS boot floppy into the floppy disk drive

The instrument will boot from the floppy and after pressing Enter to accept date/time, you can use it as a DOS computer, where floppy is A: or B:, and SanDisk (Hard Disk) is C:


This mode gives facilities to the Service Personnel to run DOS functions (Scandisk) for checking the Hard Disk, the main board, and the floppy disk drive

C#' 2#3' 7#"F%3' 3#' &%3' I)$S' <)"*' C5&S' 75"&3' 02*%"' I##3' #"*%"' &%$35#2H' 52' 3E%' N)G' &D%$575%*

)I#O%H'#3E%"N5&%'A#73N)"%'=DF")*%'N588'2#3'N#"SH')2*'3E%'&G&3%1'N588'E)2F'57')'78#DDG'*5&S

N588'I%'8%73'52'3E%'78#DDG'*"5O%'*0"52F'&3)"3U0DK

_H_) 0BBJB)C6--:M6-

The analyzer checks the operation of several mechanic, fluidic and electronic parts during measurement. The system shows the type of the error on the LCD display if any kind of malfunction is detected.

The electronic parts have a very little chance to fail, only the connections and cables could get disconnected, which can cause malfunction of the electronic system. The mechanic and fluidic system have more chance to go wrong because it has moving parts.

_H_H=) 0BBJB)DJ56)+4-8

%s: filename concerned, %d: error type concerned, %u: error specific string

%J56 0BBJB)86e8 ?6:-J;]-^

=WWW Out of memory in %s

Fatal system or program error!

?6C65E

Check the MPNIF card and DimmPC for proper insertion.

Try to make a software upgrade.

=WW=

=WWK

=WWL

=WWT

==WW

==W=

Error %d on opening file

%s

Cannot create file %s

Data file %s is corrupt, new will be created, all stored measure data will be lost

Error %d on indexing file

%s

Fatal pressure error.

Power off the system!

Waste is full!

Empty waste container!

The system assumes that you will do it before going on!



Stored data file is corrupt or missing!

Disk or software error.


Cannot make the measuring vacuum!!

Waste container is full!

The same as in case of error 1000.


New stored data file will be created automatically (by User confirmation).


Check the pump and the tubes, fittings and valves around the puffer reservoir.

Empty waste container! If it is not full, there is a clogging in the waste sensor line

(maybe Valve15 is not closed completely), or tube is pinched.

==WK

==WL

Check Diluent container!

Check Lyse container!

Diluent reagent container become empty, or the diluent fluid sensor is too sensitive or there are a lot of bubbles in the diluent tubes!

Lyse reagent container become empty, or the lyse fluid sensor is too sensitive or there are lot of bubbles in the lyse tubes!

Replace the Diluent reagent container with a filled one.

If this error still remains with a filled diluent reagent container, adjust the sensitivity of the diluent sensor and check the tubings and the fittings.

Replace the Lyse reagent container with a filled one.

If this error still remains with a filled lyse reagent container, adjust the sensitivity of the lyse sensor and check the tubings and the fittings.


%J56

==WT

==W[

==W_

==WV

0BBJB)86e8

Timeout. Try cleaning!

Vacuum error

%s – Fatal pneumatic error: %s

CMD: %u


Example:

“MVert: Ra error”

Check Cleaner container!

?6:-J;]-^

The float in the volume opto board did not reach the start or the stop position within a specified time!

?6C65E

1. Try cleaning (to remove clog).

2. Check the proper operation of the volume opto board and the float (opto switches, float, float house).

3. Check the fittings and the tubes around the volume opto board.

The measuring vacuum has been dramatically decreasing during the measurement or absolutely lost!

Motor error. One of the stepper motors could not reach a specified position, either it has not reached one of the end-switches, or it reached one without expecting to do so.

Cleaner reagent container became empty, or the dil./clean fluid sensor is too sensitive or there are lot of bubbles in the cleaner tubes!

Leakage in the pneumatic system (check the pump, fittings, tubings, pressure sensor, puffer reservoir and valves).

Check the proper operation of the motor modules (home/end opto switches, ribbon cables and the motor moving).

MDilu = dilutor, MVert = vertical,

MHori = horizontal motor

St = step error, Ra = range error

Possibly error due to lost steps of the corresponding stepper motor.

Replace the Cleaner reagent container with a filled one.

If this error still remains with a filled cleaner reagent container, adjust the sensitivity of the dil./cleaner sensor and check the tubings and the fittings.

=KWW

=KW=

=LWW

=LW=

Fatal “MeasInit” error.


Fatal HGB error.


Cannot write archive data! Check disk! It may be unformatted, full or bad! Do you want to retry?

Cannot read archive data!

Do you want to retry?

Cannot load archive data.

Fatal MPNIF error.

HGB channel did not give a ready signal!

Cannot write archive data because some disk errors!

Archive disk is damaged or not an archive disk!

Disk is not an archive disk or damaged!

Replace the MPNIF board.

Replace the MPNIF board.

Replace the floppy disk.

Change the floppy disk (archive).

=LWK

=TWW Printer error %d Printer error!

=TW=

Printer is out of paper and error %d

Printer is out of paper and there are some other printer errors!

=TWK Printer is out of paper Printer is out of paper!

=TWL

Printer was not set up correctly

The selected printer type does not match to the printer!

=TWT Paper width is too large

Paper width steps over the margin!

Change the floppy disk (archive).

Check the connections between the instrument and the printer and check the printer setup in the Settings/Printer settings sub-menu.

Feed the printer with paper. Check the connections between the instrument and the printer and check the printer setup in the Settings/Printer settings sub-menu.

Feed the printer with paper.

Modify the printer setup in the

Settings/Printer settings sub-menu.

Modify the paper setup in the



%J56

=TW[

0BBJB)86e8

Paper height is too large

=TW_ Paper width is too small

=TWV

=TWU

=TW`

=T=W

=T==

Paper height is too small

Error #%d in printer initialization!

?6:-J;]-^

Paper height steps over the margin!

Paper width steps over the margin!

Paper height steps over the margin!

Left margin is too large Left margin is too large!

Top margin is too large Top margin is too large!

Spacing is too large

The distance between two results is too high!

Printer software error!

?6C65E







Modify the margin setup in the


Modify the margin setup in the


Modify the vertical spacing setup in the

Settings/Printer settings submenu.

Check the connections between the instrument and the printer and check the printer setup in the Settings/Printer settings sub-menu.

Try to make a software upgrade.

=`WW

You have to add at least one user to use multi user mode!

Multi-user problem

Follow the instruction, or change to single user mode in the User settings submenu.


_H_HK) #6--:M6)DJ56)+4-8

%J56 #6--:M6)86e8

[WWW Are you sure to prepare the device to delivery?

[WW= Remove reagent tubing at rear reagent inputs (Diluent, Lyse, and Cleaner).

[WWK Connect min. 100 ml distilled water to reagent inputs using cleaning tube kit.

[WWL Remove cleaning tube kit. Keep reagent inputs free.

[WWT You can power off the system!

[WW[ Apertures are partially clogged. Try cleaning!

[WW_ Please empty waste container!

[=WW %u data record(s) will be copied to the database.

[=W= %u data record(s) is selected.

[=WK Insert data disk!

[=WL Insert data disk #%d of %d!

[=WT %u data record(s) will be saved on %d disk(s).

Insert an empty floppy disk!

[=W[ Insert next empty floppy disk!

[=W_ No such type of data

[KWW Your previous QC data will be lost.

Are you sure?

[LWW Some or all of the calibration factors are out of range!

[`WW This function is not available because there are no accepted blank values.

Try blank measurement!

[`W= Insert software update disk!

The system will restart.

[`WK Are you sure to delete all device statistics?

[`WL This function will delete all measured results.

Are you sure?

[`WT This function will delete all selected records.

Are you sure?

[`W[ Are you sure?


_HV) *BJ9+6C-)B6+:865)8J)d:B;4;M)7+:M-

In this section we give you examples and troubleshooting of common problems.

*BJ9+6C

WBC blank measurement error,

C, B warning flags displayed, but the

WBC histogram is correct (no noise).

There is no backlight in the

LCD Module.

All measured parameters are 0, but HGB result is correct.

Analyzer always exits from the measurement with

“Timeout error”.

WBC noise during measurements

(blank and normal).

Noise in the WBC histogram, S warning flag.

*J--49+6)B6:-J;

The instrument measures the probe voltages for both apertures during the measurements. From these values (and from the measuring times) the analyzer can detect the clogging (C, c warning flags). If the WBC probe voltage is changed, the instrument will give a C warning flag, and the

WBC value will have an E (error) flag.

This fluctuation of the probe voltage can happen if the diluent reagent temperature is changed or there is a passage of the measuring current next to the aperture. It can be caused by bad aperture or the WBC chamber is damaged.

If the LCD is blank, and the Self test shows electrode voltage and current is low, there is no high voltage in the system. The problem is that the inverter circuit that generates this high voltage is out of order, or there is no connection between the MPU-Amplifier-High Voltage boards.

The high voltage board may be damaged.

"

"

?6C65E

Replace WBC measuring tube

(aperture).

Check the WBC chamber and replace it, if it is damaged.

" Check the 34-pin and the

20-pin ribbon cables between the MPNIF –

Amplifier – High Voltage boards. Reconnect if you find something loose.

" Replace the High

Voltage Board (HVB).

" Reconnect the shielded cable at both ends

(MPNIF and Amplifier).

Shielded cable that connects MPNIF board to the

Amplifier board is disconnected. In that case only the measured HGB value is available, because it comes through the ribbon cable.

The volume meter tube is not in lowest point.

1. If there is not a good grounding in the system, the instrument can give such kind of errors.

!"#$%&"'()*/"2F!"#$%&"'( has a shock protecting system that requires earth grounding.

You must not operate the system without grounding.

2. This error can happen also if some other instruments (X-ray, monitor) are working near the instrument by making strong radiation, or from the same electrical outlet by generating fluctuation in the line, which causes noise.

3. Some extreme conditions (other reagent systems); this problem can happen with good grounding as well. In that case the blood samples make a thin fluidic film in the WBC draining tube

(lipids + lysing reagent). This fluidic film conducts the noise to the WBC measuring electrode. To prevent the system from this problem, we recommend to use the built-in Autoclean procedure, which cleans up the fluidic film from the WBC draining tube with the cleaner reagent after every Xth measurement. The default value for the Autoclean cycles is 20 and it can be changed only from the Service menu.

" Move the volume meter tube to the lowest position and check the orientation of the float within the tube.

" Measure quality with a voltage meter by measuring AC voltage between grounding and neutral.

This voltage must be less than 1 Volt. If the mains outlet does not provide a good ground, you can make it by connecting

!"#$%&"'(

*/"2F!"#$%&"'( to a grounded conductor i.e. water tap, or other grounded piping.

" If it is possible, find another place for

HUMACOUNT PLUS /

HUMACOUNT, which is not near the other instruments. Use your analyzer with an individual outlet or use line filters.

" You should lower the

Autoclean setting to 10.


_HU) *J--49+6)%:,-6-)J7)'J4-6

Generally high count of any particle - even if you think it should be low, or near zero - can be caused by NOISE, i.e. something interferes with measurement.

The most important thing in these cases to identify the source of the NOISE, otherwise we cannot protect the system against it.

NOISE can come from has several sources, and the different NOISE sources are added.

Sometimes we have to fight one of them, but sometimes more. Only one of them is enough to make problem.

_HUH=) %J;8:C4;:865)B6:M6;8

The most probable cause: real particles are in the reagent, and therefore the PLT blank is continuously high (e.g.

always 30-40). You can easily sort out this case by replacing diluent by opening a new tank. PLT blank must go down is several blank measurements (below 10).

Another reason - which is HUMACOUNT specific: the PVC tube between the T-fitting – coming from nozzle 2 of

Valve 5 – and nozzle 2 of Valve 12 should have a minimal length of 40 cm.

Please, check the length of this tube, because if a short tube is installed only, and the cleaner is contaminated, the PLT blank could not go down due to the diluent mixing with contaminated cleaner. This reason can be sorted out easily: replace the cleaner reagent with diluent, and PLT blank should go down.

!JN)D:;):)MJJ5)B6:M6;8)96DJC6)9:5)9E)84C6k

$ If the reagent tube was contaminated, and some bacteria begin to grow inside, once you put an infected reagent tube into a new tank, by time it can become infected as well, i.e. the background (PLT blank) becomes high. Wash the reagent tube - which is in connection with the reagent - with 1% of bleach solution, then rinse with clean distilled water or diluent. It can avoid the bacteria to grow inside.

$ If tank is open – and cap is not installed or closed - external dust can make reagent dirty.

_HUHK) .:5)6:B83)MBJ,;54;M

In this case external - ground referenced - noise can get into the system by ground coupling. If system ground is not good enough, ground terminal can become a noise source as well, i.e. external signals will be coupled into the system instead of protecting it.

If no earth ground is available, you can use the 4 mm diameter screw at the rear panel to connect a ground potential to the case, so that noise immunity can be increased.

Measure voltage on ground terminal to make sure earth grounding is correct. AC voltage lower than 1V is accepted in this case.

At some places - as a bad practice - electricians like to connect earth ground terminal to neutral wire. Depending on the resistance of the neutral back wire (where it is really earthed), several volts can appear, and this way any inductive noise will be coupled into the instrument. It is better to create a real earth grounding and connecting it to the rear screw.

_HUHL) 0e86B;:+)6+6D8B4D:+);J4-6

If another instrument near HUMACOUNT can radiate electromagnetic signals in the 1 kHz - 100 kHz frequency region it can be picked up by the system (especially if they are very close to each other, or the grounding is not quite perfect).

You can easily identify this noise source: by relocating the instrument noise (high PLT blank) disappears. In this case you have to identify the possible noise source (switch mode power supplies, computer monitors, since they are not shielded, centrifuges due to high switching noise of rotor contacts, etc.), the power of the electromagnetic source, because if high power is present, maybe relocation does not solve your problems, sometimes the electric power supply makes the coupling, so UPS solves the problem.

Another source of coupling in external noise can be the reagent tanks and tubes. Especially radio transmitters can cause problems of radiating so that even the reagents (diluent) guides in the noise. A metal pack for the diluent tank, then a good earth grounding of this metal box allows the coupling to disappear forever.


_HUHT) >;86B;:+);J4-6)-J,BD6-

The most annoying but real cause is some sort of internal noise. The reason for this phenomenon is that the inside electrode - hot point - of the measuring circuit must be well insulated from the surrounding electronics, otherwise inside noise sources can take their effect.

$H).:5)D3:C96B)4;-,+:84J;I

$ 9:5) -346+54;M) J7) 836) D3:C96B) (floating shield couples signals to the chamber, and does not prevent against them). Check grounding of shield, remove it and clean the surface between the shield and the metal base.

$ 9:5)B676B6;D6)6+6D8BJ56)DJ;;6D84J;)(floating ground reference). Repair is required.

$ 9:5)-6:+4;M)J7):A6B8,B6. Replacement of measuring tube is required.

$ 9BJ<6;)C6:-,B4;M)D3:C96B)starts to conduct through the gaps (ground path). Replacement of chamber is required.

$ DJ;8:C4;:865)5B:4;4;M)8,96)starts to conduct due to protein or lipid build-up. It is very easy to identify this case. After replacing the drain tube of the measuring chamber (mainly WBC), WBC histogram peak, or PLT becomes low soon. Normally a good cleaner is required to dissolve lipid or protein build-up. Sometimes the cleaner is not strong enough to keep this tube clean enough. Periodic washing using 1% hand warm bleach solution helps.

.H).:5)4;-,+:84J;)J7)6+6D8BJ;4D)-4M;:+)A:83-I

In these cases check for any capacitive coupling of electronic signals to the chamber:

$ 4;86B76B6;D6) N483) !Q.) 36:5) (high-frequency signal is coupled to the chamber). HGB head metal parts must be grounded. The ground comes externally, it must be in place, otherwise HGB head does not shield, but couples in noise.

$ 4;86B76B6;D6)N483)4;86B;:+)34M3)GJ+8:M6)4;G6B86B)(high-frequency signal is coupled to the chamber). Repair is required: avoid near contact of HVB cable to chamber or shielded amplifier cable.

$ 4;86B76B6;D6)N483)4;86B;:+)-8:B8)9,88J;)(polling signal to start button may cause noise). Guide start button wires as far from chamber as possible. You may try mix them up on the start micro-switch if applicable.

$ 4;86B76B6;D6)N483)54-A+:E)D:9+6)(high-frequency LCD signal is coupled to the chamber by the ribbon cable).

Keep the ribbon cable far from the chamber.

$ 4;86B76B6;D6) N483) %*") 7:;) JB) J836B) 54M48:+) +JM4D) 8B:D6-) (CPU fan or other digital signal radiates to chamber or to the shielded amplifier cable). Try keeping the ribbon cables far from the chamber and shielded cable.

%H).:5)DJCAJ;6;8-R)JB)DJ;;6D84J;-I

$ 9:5)-J+56B4;MR)-:+8)B6-45,:+-)JB)DJCAJ;6;8)7:4+,B6)J;):CA+4746B)(especially if some reagent could get in the amplifier section). Cleaning of PCB/electrode socket or replacement of amplifier is required. Check for the correct soldering of reference cable and its connector.

$ #*'>1) 9J:B5) 9:5) -J+56B4;M) JB) DJCAJ;6;8) 7:4+,B6. Check the shielded cable connections as well.

Sometimes inside out connection (the hot electrode goes outside as a shield) is the problem: both ends of amplifier signal cable must be reversed.

$ :;:+JM)-4M;:+)B499J;)D:9+6)(it picks up noise). Check the ribbon cable between the MPNIF board and the amplifier. Maybe it is pinched under some screws or components. This may cause trouble and even noise.

@H)*;6,C:84D)7:4+,B6-R)+4b,45)A:83-)83:8)DJ;5,D8);J4-6)4;8J)836)D3:C96BI

$ +4b,45) B6C:4;-) ,;56B) 836) D3:C96B) 4;) 5B:4;) 8,96) (during measurement the conducting liquid remains inside the drain tube making noise to appear there).

# Check chamber draining path for clogging or salt crystals.

# Check the pump operation. Since draining of the chamber goes under pressure control, maybe a bad pressure sensor or connection can cause trouble.

# Clean the draining path. Do not use alcohol, but bleach. Replace chamber if necessary.

$ +4b,45) B6C:4;-) 4;) 836) N:-34;M) 4;+68) :8) 8JA) J7)836) D3:C96B) (during measurement the conducting liquid remains inside the chamber wash tube making noise to appear). The software is not compatible with the mechanics, or related valve is bad/partly clogged, or the tubing is clogged/loose.

$ +E-6)A:83)M,456-)4;);J4-6)(during counting, if the a liquid in the draining tube is touching lyse reagent in Tfitting, noise can appear). Check the lyse path, and the lyse valve as well.


V) #$>'(0'$'%0

VH=) d66<+E)#:4;86;:;D6)9E)"-6B

User should carry out on the first workday, before starting up the analyzer.

VH=H=) %+6:;4;M)836)N:-34;M)36:5

User should clean the lower surface of the aspirating tip washing head using a soft cloth, immersed in warm tap water to remove salt build-up.

VHK) *6B4J54D)#:4;86;:;D6)9E)26BG4D6

The instruments should be checked and maintenance must be carried out in every 6-12 months, or after

10 000 measurement cycles.

VHKH=) %36D<)26+7)86-8):;5)@6G4D6)-8:84-84D-

Run the built-in Self test and check the overall test result. Check the device statistics to find common problems.

VHKHK) %+6:;4;M):;5)QB6:-4;M)@4+,8JB).+JD<

The dilutor block driving wheels and gear bar should be cleaned from dirt and must be greased between the gear bar and the support, and between cogged wheels.

VHKHL) %36D<4;M):;5)/,9B4D:84;M)@4+,8JB)*4-8J;)(4A-

The cogged end of PTFE dilutor pistons should be cleaned and lubricated by neutral silicon grease.

Apply just a thin layer, and move it along the perimeter of the piston, so that some of the material goes into the gaps between the sealing rings.

Repeat this step for lyse and dilutor pistons as well. Check the condition of the micro piston sealing, and replace if necessary.

VHKHT) %+6:;4;M):;5)/,9B4D:84;M)'665+6)#JG4;M)#6D3:;4D-

The H&V moving mechanics sliding bars should be cleaned from dust.

Lubricating of the sliding bars must be made using „*3J8JB,96” oil containing PTFE. QB6:-6)JB) A,B6

+,9B4D:84;M)J4+)4-);J8)-,48:9+6H

VHKH[) %36D<4;M):;5)?6A+:D4;M)d:-34;M)!6:5

Check the state of the washing head, and replace if necessary.

Perform correct adjustment of the needle height (see Section 4.1.2).

VHKH_) %36D<4;M):;5)?6A+:D4;M)*6B4-8:+84D)*,CA)(,96

Replace peristaltic pump tube if needed. You can check it by opening the lock, and removing the tube for inspection.

Check for leakage of the tubing. Reassemble the head.

L)"252FM'(%'$)"%708H'C-':-?'3N5&3'3E%'30I%'NE58%'"%D#&535#2'53'523#'3E%'E%)*H'I%$)0&%'53

N588'$)0&%'1)8702$35#2'52')'O%"G'&E#"3'D%"5#*'#7'351%K

VHKHV) %36D<4;M)J7)836)*JN6B)2,AA+E

Open the instrument for regular cleaning inside. Check the cleanliness and operation of the cooling fan of the power supply. Clean or replace the fan if necessary.

VHKHU) .+6:D34;M)J7)1+,454D)2E-86C

It is recommended to run a bleaching procedure to remove stains from the fluidic system.

1. Connect 2-5%, hand warm, clean bleach solution to all reagent inputs, and perform priming on all reagent inputs.

2. Leave it in the tubing for not more than 2-3 minutes.

3. Remove bleach, and prime all on air.

4. Connect distilled water (100 ml), and perform priming all reagents, again.

Connect reagents, and run priming again.


U) 2*$?0)*$?(2

%&@0 '$#0

A130 Front panel (HUMACOUNT PLUS, complete)

A131 Front panel (HUMACOUNT PLUS, excl. keyboard)

A132 Foil keyboard (HUMACOUNT PLUS)

A133 HVB board with inverter (complete)

A136 LCD display

A137 Lamp for LCD

A140 Amplifier board (HUMACOUNT PLUS, complate)

A154 Floppy drive

A156 Power supply

A157 Mains switch

A159 Loudspeaker

A160 MPNIF board (complete)

A170 IDEEPROM board

A191 MB4 motherboard for DimmPC

A192 DimmPC (CPU)

A193 Heat sink for DimmPC

A194 Connector holding plate

A195 DimmPC safe ring

A196 MB4 motherboard holding plate (HUMACOUNT PLUS)

A300 XY needle movement block (complete)

A301 Timing belt for XY block

A302 Stepper motor

A303 Bearing for XY block

A304 Timing pulley for X (mounted)

A305 Timing pulley for Y (mounted)

A306 Gear for Y opto

A307 H&V motor opto board (complete)

A308 Opto switch I.

A309 Opto switch II.

A310 Head washer

A311 Aspirator tip

A313 Seeger ring (D 19)

A314 Seeger ring (RA5)

A321 Return pulley for XY

A400 Meas. Block excl. Amplifier (HUMACOUNT PLUS)

A401 Cone for chambers

A402 Sealing ring for chamber

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%&@0 '$#0

A403 U-shaped metal fixing for apertures

A404 Cone for WBC chamber

A405 Shield for WBC chamber

A410 MIX chamber

A420 RBC chamber

A421 RBC aperture (80 µm)

A422 Ground electrode for RBC/WBC

A430 WBC chamber

A431 WBC aperture

A433 Upper part for WBC chamber

A434 HGB meas. head ( complete )

A440 Complete VMOP board (A441+A442)

A441 VMOP volume limiter board (only PCB)

A442 Volume limiter tube

A500 Pneumatic block excl. measuring head, DIL, XY

A501 Valve unit I. (HUMACOUNT PLUS,compl. valve 1-5)

A502 Valve unit II.(HUMACOUNT PLUS,compl. valve 6-9)

A503 Valve u. III.(HUMACOUNT PLUS,compl.valve11-15)

A504 2/2 valve

A505 3/2 valve

A506 Valve coil for both valves

A507 2/2 valve head

A508 3/2 valve head

A510 Puffer reservoir HUMACOUNT PLUS

A511 Puffer reservoir holder (pair)

A530 PCPRO pressure board (complete)

A531 Pressure sensor

A532 Reagent sensor tube for PCPRO board

A541 4/2 plastic tube (silicon)

A542 5/3 plastic tube (silicon)

A543 4/1.8 Tygon tube

A544 5/3 reagent tube

A545 3,2 mm T connector

A546 Y connector

A547 2,3/3,2 fitting

A548 O ring for WBC chamber

A549 O ring for apertures


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A550 Diluent cont. connector

A551 Colour locking ring

A552 Colour lock nut (inside)

A553 Colour coding ring (outside)

A554 Luer female

A555 Luer male

A556 2,3 mm T connector

A557 Teflon regent cont. Connector

A558 Reagent tubing set (HUMACOUNT PLUS)

A559 Reagent tubing set (HUMACOUNT)

A560 Peristaltic pump

A561 Tube for peristaltic pump

A563 Cassette for pump

A564 Fixing for pump (pair)

A565 Connector for pump (pair)

A600 Body (HUMACOUNT PLUS, mounted)

A610 Micro switch

A611 Sampling bar (HUMACOUNT PLUS, mounted)

A612 Gloving LED cover (HUMACOUNT PLUS, plastic)

A613 Gloving LED cover board (complete)

A620 Instrument door (HUMACOUNT PLUS)

A623 Magnet for door

A630 Upper body cover (HUMACOUNT PLUS, plastic )

A640 Instrument foot

A650 Screw for loudspeaker

A660 HUMACOUNT PLUS cover with HUMAN logo

A661 HUMACOUNT PLUS cover without logo

A662 HUMACOUNT cover with HUMAN logo

A663 HUMACOUNT cover without logo

A701 Mains cable

A712 X motor cable (HUMACOUNT PLUS)

A713 Y motor cable (HUMACOUNT PLUS)

A714 Dilutor cable (HUMACOUNT PLUS)

A715 Keyboard cable (HUMACOUNT PLUS)

A716 LCD cable (HUMACOUNT PLUS)

A717 Valve I. (1-5) cable (HUMACOUNT PLUS)

A718 Valve II. (6-10) cable (HUMACOUNT PLUS)

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A719 Valve III. (11-15) cable (HUMACOUNT PLUS)

A720 Pressure board cable (HUMACOUNT PLUS)

A721 Amplifier board cable I. (HUMACOUNT PLUS)

A722 Amplifier board cable II. (HUMACOUNT PLUS)

A723 HVB board cable (HUMACOUNT PLUS)

A724 COMS&KEY connector PCB

A725 COMS&KEY ribbon cable

A726 LPT&USB connector PCB

A727 LPT&USB ribbon cable

C130 Front panel (HUMACOUNT, complete )

C131 Front panel (HUMACOUNT, excl. keyboard)

C132 Foil keyboard (HUMACOUNT)

C140 Amplifier board (HUMACOUNT, complete)

C196 MB4 motherboard holding plate (HUMACOUNT)

C400 Meas. Block excl. Amplifier (HUMACOUNT)

C406 Upper part for HUMACOUNT chamber

C500 Pneumatic block excl. measuring head, DIL, XY

C501 Valve unit I. (HUMACOUNT, complete, valve 1-5)

C503 Valve u. III. (HUMACOUNT, complete, valve11-15)

C510 Puffer reservoir HUMACOUNT

C600 Body (HUMACOUNT, mounted)

C611 Sampling bar (HUMACOUNT, mounted)

C612 Gloving LED cover (HUMACOUNT, plastic)

C620 Instrument door (HUMACOUNT)

C621 Joint-hinge for door (HUMACOUNT)

C624 Body cover (HUMACOUNT)

C630 Plastic front body (HUMACOUNT, plastic)

C712 X motor cable (HUMACOUNT)

C713 Y motor cable (HUMACOUNT)

C714 Dilutor cable (HUMACOUNT)

C715 Keyboard cable (HUMACOUNT)

C716 LCD cable (HUMACOUNT)

C717 Valve I. (1-5) cable (HUMACOUNT)

C719 Valve III. (11-15) cable (HUMACOUNT)

C720 Pressure board cable (HUMACOUNT)

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C721 Amplifier board cable I. (HUMACOUNT)

C722 Amplifier board cable II. (HUMACOUNT)

C723 HVB board cable (HUMACOUNT)

S200 Dilutor block (complete)

S203 Micro syringe

S204 Micro piston

S205 Micro piston sealing part

S209 Track

S210 Bearing for dilutor

S211 Seeger ring

S214 Dilutor panel ( mounted )

S221 Macro syringe+piston

S222 Lyse syringe+piston

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`) $**0'@>%02

`H=) d:B;4;M)1+:M-

1+:M #6:;4;M ?6DJCC6;565),-6B):D84J; d

0

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#

@

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WBC three part warning or WBC three part diff. Unsuccessful

No WBC three part

HGB blank is high, or no HGB blank

WBC blank is high, or no WBC blank

WBC/RBC limit warning

Too many RBC cut from WBC

WBC coincidence is too high. Linearity error.

WBC data package errors

WBC time error

WBC clogging

PLT blank is high, or no PLT blank

% Repeat the measurement. Possibly lyse problem.

% Check the discriminators in the WBC histogram. If the discriminators are in the proper place (the populations can be separated by eye) then the results are correct.

% Possibly lyse problem, but in some pathological samples (too high lymphocytes), it can be happened.

% Repeat the blank measurement and accept it.

% Possibly lyse or diluent problem.


% Possibly lyse or diluent or noise problem.

% Check the 1. RBC-LYM discriminator. If it is in the minimum point (or close to it), accept the results. Otherwise repeat the measurement.

% If the retried action makes very similar results and the discriminator is in a wrong place then the MID and GRA results are OK, but the WBC and

LYM results can be higher because of the RBCs.

% Repeat the measurement. Possibly lyse problem.

% If the WBC measuring time is too high (more than 8 sec.) it could be aperture clogging. In that case perform cleaning and repeat the measurement.

% The results are out of the linearity range. Make a dilution with an external dilutor with a pre-defined dilution range. Do not forget to correct the results with the defined factor.

% Perform cleaning and repeat the measurement (aperture clogging).

% If it is a general problem, please call your Service Personnel.

% The same action as in case of the @ warning flag.

% Aperture clogging. The same action as in case of the @ warning flag.


% Diluent problem. Replace diluent, open a new tank.

.

/

RBC blank is high, or no RBC blank

RBC/PLT limit warning


% Diluent problem. Replace diluent, open a new tank.

% The RBC/PLT valley is too high. It is rather a diagnostic flag. If the discriminator is in a wrong place (in the PLT or RBC histogram) then repeat the measurement for a correct PLT result.

a RBC peak warning

# RBC/PLT coincidence is too high. Linearity error.

% Perform cleaning and repeat the measurement (clogging).

% If it is a general problem, change the RBC aperture.

% The same action as in case of the # warning flag.

@ RBC/PLT data package errors

% The same action as in case of the @ warning flag.

2 RBC/PLT time error % The same action as in case of the @ warning flag.

% RBC/PLT clogging % The same action as in case of the % warning flag.


`HK) 26B4:+)%JCC,;4D:84J;)*BJ8JDJ+)]SKHe^

`HKH=) Q6;6B:+)@6-DB4A84J;

The HUMACOUNT PLUS/HUMACOUNT software is able to make serial connection link to a receiver device

(e.g. computer) which is connected to the serial port. If the Baud Rate setting in the Service Menu is set to a valid value (other than Not Connected), the instrument will try to initiate a communication sequence.

The serial communication is always initiated by HUMACOUNT PLUS/HUMACOUNT, and the receiver must respond to this.

The hardware protocol: U)5:8:)948R) =) -8JA) 948R) ;J)A:B48E. If the other side is not responding in 1 second, the instrument will repeat the transmission twice, but if still no response, the other side will be supposed to be not ready to receive data, and thus HUMACOUNT PLUS/HUMACOUNT will not try to communicate any more.

From this state it can wake up by receiving an <ENQ> (ASCII code 5) character from the other side, and the instrument will immediately respond an <ACK> (ASCII code 6). From this point the instrument will send data if it is ready for transmission.

The communication is based on packages. There are 5 package types:

! INIT package: Device identification, software version, current date and time.

! DATA package: Sample and patient information, measured parameters, and markers.

! RBC package: Sample information, RBC histogram.

! WBC package: Sample information, WBC histogram.

! PLT package: Sample information, PLT histogram.

The communication sequence is always started with an INIT package. If the link is successful, the instrument will send DATA package, and the receiver can request RBC, WBC and PLT packages at acknowledge.

Special characters used in the communication:

Character

<SOH>

<STX>

<ETX>

<EOT>

<ENQ>

<ACK>

<HT>

<LF>

<NAK>

<SPACE>

ASCII code

1

2

3

4

10

21

32

5

6

9


`HKHK) 1JBC:8)J7)*:D<:M6-)26;8)9E)!"#$%&"'()*/"2F!"#$%&"'(

The packages sent by HUMACOUNT PLUS/HUMACOUNT are always between a <SOH> and <EOT> character, and they consist of header, data and tail. The header consists of a package identifier and a package type descriptor. The tail includes the checksum. Typical format:

p2&!qMID CMDp2(fqMESSAGEp0(fqCHKSUMp0&(q

MID:

CMD:

Message ID, one capital letter between ’A’ and ’Z’

Command, one capital letter, package type descriptor

! ’I’ for INIT;

! ’D’ for DATA;

! ’R’ for RBC;

! ’W’ WBC;

MESSAGE: the message consists of ASCII characters (between 32..128)

CHKSUM: two hexadecimal digits of a one-byte checksum which contains the lower byte of the sum of characters between the <SOH> and <ETX> (incl. Those, too).

`HKHL) 1JBC:8)J7)$D<;JN+65M6)J7)836)?6D64G6B

The receiver must acknowledge transmission by the following message:

p$%aqCMD MID

CMD:

MID: the type of the next package to send – this makes possible to a request for histograms. If no more packages are required, <SPACE> should be sent.

the identifier of the package that acknowledged by this message.

If receiving of the message was not successful, <NAK> should be sent, and HUMACOUNT PLUS/HUMACOUNT will repeat the last package.

The receiver has approx. 1 second to reply, otherwise HUMACOUNT PLUS/HUMACOUNT will repeat the last transmission, twice automatically. But if still no response after 3 trials, HUMACOUNT PLUS/HUMACOUNT will not start to communicate any more, even if there is data to send (see General Description).

`HKHT) @68:4+65)@6-DB4A84J;)J7)*:D<:M6-

The packages sent by HUMACOUNT PLUS/HUMACOUNT are placed between a header and a tail. There are 3 main types of them: INIT, DATA and histogram packages. The histogram package can be: RBC, WBC, and PLT.

Their format is the same, the type descriptor differs only.

`HKH[) >'>()A:D<:M6

HUMACOUNT PLUS/HUMACOUNT sends the general identifiers by this package during initiation of a communication:

DEVICEp!(qVERSIONp!(qDATEp!(>TIME

DEVICE: device identifier

VERSION: version of the software, e.g. „2.7”

DATE: date in YYYYMMDD format

TIME: time in HHMMSS format


`HKH_) @$($)A:D<:M6

This package contains sample and patient data, the measured parameters and the markers of histograms. One data element consists of two items: name and value, which are separated by a <HT> character, and closed by

<LF>.

61D#"3)23M'A:-H'C>?]H'?6.]H'A6C')2*'!6C'75%8*&'N588')8N)G&'I%'D"%&%23'52'3E5&'#"*%"H'I03'#3E%"'75%8*&'1)G

I%'#1533%*K

SNOp!(q152p/1q

DATEp!(q19980715p/1q

TIMEp!(q114500p/1q

SIDp!(q1AXX435p/1q

PIDp!(qB72D44p/1q

NAMEp!(qJOE SMITHp/1q

BIRTHp!(q19650208p/1q

SEXp!(q0p/1q

DOCp!(qDr John Goldp/1q

OPIDp!(q1172p/1q

MODEp!(q0p/1q

WRNp!(q0p/1q

PM1p!(q12p/1q

PM2p!(q204p/1q

RM1p!(q51p/1q

WM1p!(q23p/1q

WM2p!(q57p/1q

WM3p!(q92p/1q

PARNp!(q22p/1q

P01p!(q 6.6p!(q0p/1q

P02p!(q4.29p!(q0p/1q

...

P22p!(q 8.2p!(q0p/1q internal identifier date of measurement (YYYYMMDD) time of measurement (HHMMSS) sample identifier (alphanumeric) patient identifier (alphanumeric) patient name (alphanumeric) birthdate of patient (YYYYMMDD) patient sex (0-male, 1-female) doctor’s name operator identifier (alphanumeric) patient type warning bits

1

(32-bit hexadecimal)

PLT lower marker

2

PLT upper marker

2

RBC lower marker

2

WBC upper marker

2

WBC LYM-MID marker

2

WBC MID-GRA marker

2 number of parameters to send first parameter ID

3,

value and flag

4 second parameter ID, value and flag last parameter ID, value and flag a%1)"S&9

1

Position and meaning of the warning flags:

.48

#:-< /6886B

0 0x00001 c

1 0x00002

2 0x00004 s d

3 0x00008

4 0x00010

5 0x00020

6 0x00040 m l k b

7 0x00080

8 0x00100

9 0x00200

10 0x00400

11 0x00800

12 0x01000

13 0x02000

14 0x04000

L

B

M

R

S

D p

C

#6:;4;M

RBC/PLT clogging.

RBC/PLT time error.

RBC/PLT data package errors.

RBC/PLT coincidence is too high. Linearity error.

RBC peek warning.

RBC/PLT limit warning.

RBC blank is high, or no RBC blank.

PLT blank is high, or no PLT blank.

WBC clogging.

WBC time error.

WBC data package errors.

WBC coincidence is too high. Linearity error.

Too many RBC cut from WBC.

WBC/RBC limit warning.

WBC blank is high, or no WBC blank.


15 0x08000

16 0x10000

17 0x20000

H

E

W

HGB blank is high, or no HGB blank.

No WBC three part.

WBC three part warning or WBC three part diff. Unsuccessful.

2

3

The markers are given in histogram channel between 0 and 255, where 0 means that the marker could not be found.

The parameter Ids in HUMACOUNT PLUS/HUMACOUNT are: P01: WBC (10

P09: PCT (%); P10: MPV (fl); P11: PDWsd (fl); P12: PDWcv (%); P13: RDWsd (fl); P14: RDWcv (%);

/l); P16: MID (10

9

9

/l); P02: RBC (10

12

P03: HGB (g/l); P04: HCT (%); P05: MCV (fl); P06: MCH (pg); P07: MCHC (g/l); P08: PLT (10

9

/l);

/l);

P15: LYM (10

9

/l); P17: GRA (10

9

/l); P18: LYM% (%); P19: MID% (%); P20: GRA%

(%); P21: RBCtime (sec); P22: WBCtime (sec).

4

The parameter value is always 4 character wide, spaces from left added if necessary. It can be 9999, if the value could not be displayed in 4 digits, or ---- if the value could not be calculated because of an error.

The meaning of the parameter flags:

1+:M

0

1

2

3

4

5

@4-A+:E65

+

$

*

E

#6:;4;M

Value correct.

Value high (more than upper limit)

Value low (less than lower limit)

Value is unreliable.

Value not given because of error. Value is ----.

Value cannot be calculated. There is no value!

J5:K#I5:#-".#)9A#N-36-,2

The format of the histogram packages are the same, they differ in type descriptor only. The package contains the sample data (to identify the package) and the histogram.

SNOp!(q152p/1q

DATEp!(q19980715p/1q

TIMEp!(q114500p/1q

SIDp!(q1AXX435p/1q

PIDp!(qB72D44p/1q

CHNp!(q256p/1q

9p!(q

...

1p!(q value of the last histogram channel internal identifier date of measurement (YYYYMMDD) time of measurement (HHMMSS) sample identifier (alphanumeric) patient identifier (alphanumeric) number of histogram channels (256) value of first histogram channel value of 255 th

histogram channel


`HKHV) (B:;-C4--4J;)0e:CA+6

Let us suppose that the receiver was not ready to receive when HUMACOUNT PLUS/HUMACOUNT was switched on, therefore the instrument is not starting a communication.

Receiver sends that it is ready to receive now, HUMACOUNT PLUS/HUMACOUNT sends acknowledge.

<ENQ>

<ACK>

HUMACOUNT PLUS/HUMACOUNT sends INIT next time to initiate link (there is data to send):

S=HV)-J78N:B6I

<SOH>AI<STX>HUMAN HUMACOUNT PLUS<HT>1.7g<HT>20010405<HT>163902<ETX>88<EOT>

SKHe)-J78N:B6I

<SOH>AI<STX>HUMACOUNT PLUS<HT>2.15a<HT>20010405<HT>160734<ETX>E4<EOT>

Receiver sends acknowledge:

<ACK><SPACE>A

HUMACOUNT PLUS/HUMACOUNT sends DATA package:

<SOH>BD<STX>SNO<HT>1<LF>DATE<HT>20010405<LF>TIME<HT>163800<LF>SID<HT>1<LF>PID<HT><

LF>NAME<HT><LF>BIRTH<HT>00000000<LF>SEX<HT>0<LF>DOC<HT><LF>OPID<HT>0<LF>MODE<HT>

0<LF>WRN<HT>0<LF>PM1<HT>10<LF>PM2<HT>135<LF>RM1<HT>34<LF>WM1<HT>21<LF>WM2<HT>55

<LF>WM3<HT>91<LF>PARN<HT>22<LF>P01<HT>6.4<HT>0<LF>P02<HT>4.36<HT>0<LF>P03<HT>131<HT

>0<LF>P04<HT>39.0<HT>0<LF>P05<HT>90<HT>0<LF>P06<HT>30.0<HT>0<LF>P07<HT>335<HT>0<LF>P

08<HT>262<HT>0<LF>P09<HT>0.24<HT>0<LF>P10<HT>9.2<HT>0<LF>P11<HT>12.0<HT>0<LF>P12<HT>3

6.9<HT>0<LF>P13<HT>52.3<HT>1<LF>P14<HT>16.3<HT>0<LF>P15<HT>2.1<HT>0<LF>P16<HT>0.5<HT>0

<LF>P17<HT>3.7<HT>0<LF>P18<HT>33.4<HT>0<LF>P19<HT>7.8<HT>1<LF>P20<HT>58.8<HT>0<LF>P21

<HT> 8.4<HT>0<LF>P22<HT> 5.0<HT>0<LF><ETX>89<EOT>

Receiver acknowledges DATA transmission, and requests for RBC package:

<ACK>RB

HUMACOUNT PLUS/HUMACOUNT sends RBC package:

<SOH>CR<STX>SNO<HT>1<LF>DATE<HT>20010405<LF>TIME<HT>163800<LF>SID<HT>1<LF>PID<HT>5

7290<LF>CHN<HT>256<LF>4<HT>9<HT>17<HT>27<HT>37<HT>48<HT>58<HT>66<HT>70<HT>72<HT>70

<HT>66<HT>60<HT>54<HT>47<HT>39<HT>33<HT>28<HT>22<HT>18<HT>15<HT>11<HT>9<HT>8<HT>7<

HT>5<HT>4<HT>4<HT>3<HT>3<HT>2<HT>2<HT>1<HT>1<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<H

T>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>1<HT

>1<HT>1<HT>2<HT>2<HT>3<HT>4<HT>5<HT>6<HT>7<HT>8<HT>9<HT>10<HT>11<HT>12<HT>14<HT>16

<HT>18<HT>21<HT>24<HT>28<HT>31<HT>35<HT>38<HT>43<HT>47<HT>53<HT>58<HT>65<HT>71<HT>

78<HT>86<HT>94<HT>103<HT>113<HT>121<HT>130<HT>139<HT>149<HT>160<HT>171<HT>184<HT>19


53<HT>253<HT>253<HT>253<HT>254<HT>255<HT>254<HT>252<HT>248<HT>243<HT>236<HT>229<HT>

221<HT>214<HT>207<HT>199<HT>192<HT>185<HT>178<HT>170<HT>162<HT>154<HT>146<HT>137<HT

>129<HT>121<HT>113<HT>104<HT>96<HT>88<HT>82<HT>75<HT>71<HT>66<HT>62<HT>58<HT>55<HT>

51<HT>47<HT>43<HT>38<HT>35<HT>31<HT>28<HT>25<HT>22<HT>19<HT>18<HT>16<HT>14<HT>12<H

T>11<HT>10<HT>9<HT>9<HT>9<HT>9<HT>8<HT>8<HT>7<HT>7<HT>6<HT>6<HT>5<HT>5<HT>5<HT>4<

HT>4<HT>3<HT>3<HT>3<HT>2<HT>2<HT>2<HT>1<HT>1<HT>1<HT>1<HT>1<HT>1<HT>1<HT>2<HT>2<H

T>2<HT>2<HT>2<HT>2<HT>2<HT>1<HT>1<HT>1<HT>1<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT

>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>

0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0

<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<ETX>60<EOT

>

Receiver acknowledges RBC transmission, and requests for WBC package:

<ACK>WC


HUMACOUNT PLUS/HUMACOUNT sends WBC package:

<SOH>DW<STX>SNO<HT>1<LF>DATE<HT>20010405<LF>TIME<HT>163800<LF>SID<HT>1<LF>PID<HT>

57290<LF>CHN<HT>256<LF>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>0<HT>255<HT>25

5<HT>255<HT>251<HT>209<HT>163<HT>118<HT>82<HT>56<HT>40<HT>32<HT>29<HT>29<HT>30<HT>3

4<HT>40<HT>47<HT>55<HT>64<HT>76<HT>88<HT>103<HT>119<HT>136<HT>155<HT>174<HT>195<HT>

215<HT>233<HT>246<HT>254<HT>255<HT>249<HT>240<HT>227<HT>213<HT>200<HT>187<HT>176<HT

>164<HT>153<HT>141<HT>129<HT>118<HT>108<HT>99<HT>92<HT>86<HT>81<HT>76<HT>71<HT>67<H

T>64<HT>62<HT>61<HT>60<HT>60<HT>60<HT>57<HT>55<HT>51<HT>47<HT>44<HT>43<HT>42<HT>42<

HT>43<HT>44<HT>45<HT>47<HT>47<HT>46<HT>45<HT>43<HT>40<HT>37<HT>34<HT>32<HT>30<HT>3

0<HT>30<HT>30<HT>29<HT>28<HT>26<HT>24<HT>21<HT>19<HT>17<HT>15<HT>14<HT>13<HT>12<HT

>13<HT>13<HT>13<HT>13<HT>13<HT>12<HT>11<HT>11<HT>11<HT>12<HT>12<HT>14<HT>15<HT>16<


3<HT>24<HT>25<HT>25<HT>25<HT>26<HT>29<HT>31<HT>34<HT>35<HT>36<HT>36<HT>35<HT>35<HT

>35<HT>37<HT>38<HT>41<HT>43<HT>46<HT>47<HT>48<HT>50<HT>52<HT>56<HT>60<HT>64<HT>70<

HT>74<HT>79<HT>82<HT>86<HT>89<HT>92<HT>95<HT>99<HT>102<HT>103<HT>104<HT>105<HT>105<

HT>106<HT>108<HT>110<HT>112<HT>115<HT>117<HT>119<HT>120<HT>121<HT>120<HT>120<HT>120

<HT>121<HT>122<HT>125<HT>129<HT>132<HT>136<HT>139<HT>141<HT>142<HT>142<HT>140<HT>13


09<HT>108<HT>108<HT>109<HT>110<HT>110<HT>108<HT>104<HT>99<HT>94<HT>89<HT>85<HT>82<H

T>80<HT>79<HT>78<HT>77<HT>75<HT>72<HT>68<HT>63<HT>58<HT>54<HT>50<HT>47<HT>44<HT>43<


3<HT>22<HT>22<HT>21<HT>21<HT>19<HT>17<HT>13<HT>9<HT>5<ETX>F7<EOT>

Receiver acknowledges WBC transmission, and closes the link. It is possible to request for PLT histogram if the acknowledge contains a “P” instead of <SPACE>, but it is not shown in this example.

<ACK><SPACE>D

`HL) ?6DJCC6;565)<48)J7)8JJ+-

! PC standard keyboard

! Screwdrivers:

Cross Slot Screwdrivers (Philips)

Slot Screwdrivers

Hexagon Screwdrivers (3.5, 2.5, 2.0, 1.5 mm sizes) digital

! Diagonal Cutter (plier)

! Nipper


d5Z\

/dZ\(L

=

5eZ\

Abacus

(

QD8&'.(;?)"%1#&?4

JXP;OKY(5W\(((5\\\W\eW/0W(((L8#D8/\WN4!

J//

5 d

/

(C gZ6 dZ\(L

/ d

5

J/

/// eZ6(L eZ6(L

F

]]Z](L(i(eS5(4&,&?+'(#D8"

]]Z](<(i(fSd(4&,&?+'(#D8"

]]Z](i(eS5(43"?&1,(=JA(#D8" hZ6

/dZ6 e/Z\

6/Z6

5hZ\

/

//Z6

6Z\ d

5

J/\

/ d

5

Jh

/5Z6

/dZ\ d

5

J0

P

0\

[

/\\

/Z6(L

/fZ\ gZ\ /0Z\

/Z6(L

/Z6(L

5

/

J5

/Z6(L

/ d

5

Jd

/Z6(L

/Z6(L

/ d

5

Je

/Z6(L

/Z6 hZ\ dZ6

5

/Z6(L

/

J6 dZ\(L eZ6(L d6Z\(L

5

/Z6(L

/

Jg

5

/Z6(L

/

Jf

/5Z\(<

55Z\ dgZ6

J/5

5 d

6Z\

/

6Z\

J/d

5 d

/

/\Z6 fZ\

C

B&,W

J/e

5 d

/

J/6

/

5

/6Z6

/5Z\

/eZ6 eZ6(L

@

5Z\(<

B

5Z\(<

=D**"$

=D%3 dfZ\ f\Z\

B

[4 dZ6(<

[

@

A

9.5 HUMACOUNT tubing schematic


PLUS Cabling Diagram


9.7 HUMACOUNT Cabling Diagram


PLUS Amplifier, High voltage & HGB Boards


9.9 HUMACOUNT Amplifier


PLUS Amplifier PCB


9.11 HUMACOUNT Amplifier PCB


9.12 H&V, DIL, VM, LD Opto and Valve Boards


9.13 Electronic part PCBs

Rev. No.

3.0

Date Person

2004.06.01.

Hans B. Kern

Related section

All

***


Description

Reviewed for new M/B

77/77

Human

Gesellschaft für Biochemica und Diagnostica mbH

Max-Planck-Ring 21 ! D-65205 Wiesbaden

Germany

Telefon:

Telefax:

+49 6122 9988 0

+49 6122 9988 100 eMail:

Internet: [email protected]

http://www.human.de

01/2004-06

HUMAN HUMACOUNT PLUS, Hematology Analyzer Service Manual

Assistant Bot

Service Manual

&

Hematology Analyzer

!

($./0)&1)%&'(0'(2

Flash

BIOS

32 Mbytes RAM

Hard Disk

(SanDisk)

SanDisk controller

CMOS

EEPROM

Real-timeclock

On-board

SMPS

Super I/O

Edgeconnector

AMD Elan

SC520

CPU

!"#$%&"'(

*/"2

!"#$%&"'(

*/"2

!"#$%&"'(

*/"2

!"#$%&"'(

*/"2

p2&!qMID CMDp2(fqMESSAGEp0(fqCHKSUMp0&(q

p$%aqCMD MID

DEVICEp!(qVERSIONp!(qDATEp!(>TIME

Receiver acknowledges WBC transmission, and closes the link. It is possible to request for PLT histogram if the acknowledge contains a “P” instead of <SPACE>, but it is not shown in this example.

Abacus

9.5 HUMACOUNT tubing schematic

PLUS Cabling Diagram

9.7 HUMACOUNT Cabling Diagram

PLUS Amplifier, High voltage & HGB Boards

9.9 HUMACOUNT Amplifier

PLUS Amplifier PCB

9.11 HUMACOUNT Amplifier PCB

9.12 H&V, DIL, VM, LD Opto and Valve Boards

9.13 Electronic part PCBs

Key Features

Frequently Answers and Questions

What is the intended use of the HUMACOUNT PLUS and HUMACOUNT analyzers?

What type of samples can the analyzers process?

What are the key parameters measured by the analyzers?

What is the throughput of the HUMACOUNT PLUS and HUMACOUNT analyzers?

What is the purpose of the integrated software?

Related manuals

Human

HumaCount 60TS

Wells Manufacturing

WBC-20

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Hemolyzer 3

Diatron

ABACUS

Diatron

Abacus junior

Innovative Control Systems

Tunnel Master wbc

Diamond Diagnostics

Prolyte

Abbott

CELL-DYN 1800

Innovative Control Systems

wbc Tunnel Master wbc

Diatron

ABACUS 4

COULTER

MicroDiff

Table of contents