Esco Labculture® Plus Class II Type A2 Biosafety Cabinet

Esco Labculture® Plus Class II Type A2 Biosafety Cabinet
ESCO
®
A Guide To Biosafety &
Biosafety Cabinets
A Guide To Biosafety And Biosafety Cabinets
Foreword
This booklet was developed as a guide to biosafety cabinets that provides basic
knowledge of biosafety and biosafety cabinets. The information presented
is unbiased and generic in nature compiled with help from experienced
microbiologists, engineers and safety enclosure users.
I. Biohazards and Biosafety Levels
The word biohazard is a contraction of the words biological and hazard,
and defined as: “an infectious agent, or part thereof, presenting a real or
potential risk to the well-being of man, animals and / or plants, directly
through infection or indirectly through disruption of the environment.”
Biosafety Levels 1 through 4 were established by the Centers for Disease
Control (CDC) and the National Institutes of Health (NIH) and are combinations
of laboratory practices and techniques, safety equipment and facilities. All of
these levels are appropriate for the biohazard posed by the agents used and
for the laboratory activity.
Biosafety Level 1
Practices, safety equipment and facilities appropriate for work with defined
and characterized strains of viable micro organisms not known to cause
disease in healthy adult humans. The laboratory is not necessarily separated
from the general traffic patterns in the building. Work is generally conducted
on open bench tops using standard microbiological practices. Special
containment equipment or facility design is neither required nor generally
used. Laboratory personnel have specific training in the procedures conducted
in the laboratory and are supervised by a scientist with general training in
microbiology or a related science. A biohazard safety cabinet is generally not
required for work involving these agents.
Biosafety Level 2
Practices, safety equipment and facilities appropriate for work done with
a broad spectrum of indigenous moderate-risk agents present in the
community and associated with human disease in varying severity. It differs
from biosafety level 1 in that:
a) laboratory personnel have specific training in handling pathogenic agents
and are directed by competent scientists;
b) access to the laboratory is limited when work is being conducted;
c) extreme precautions are taken with contaminated sharp items; and
d) certain procedures in which infectious aerosols or splashes may be
created are conducted in biological safety cabinets or other physical
containment equipment. A Class I or Class II biohazard safety cabinet is
highly recommended for work involving these agents.
Biosafety Level 3
Practices, safety equipment and facilities appropriate for work done with
indigenous or exotic agents with a potential for respiratory transmission
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A Guide To Biosafety And Biosafety Cabinets
which may cause serious and potentially lethal infection. More emphasis
is placed on primary and secondary barriers to protect personnel in the
contagious area, the community, and the environment from exposure to
potentially infectious aerosols. A Class I or Class II biohazard safety cabinet
is required for work involving these agents.
Biosafety Level 4
Practices, safety equipment and facilities appropriate for work done with
dangerous and exotic agents which pose a high risk of life threatening
disease. May be transmitted via the aerosol route, and for which there is no
available vaccine or therapy. Members of the laboratory staff have specific
and thorough training in handling extremely hazardous infectious agents
and they understand the primary and secondary containment functions
of the standard and special practices, the containment equipment, and
the laboratory design characteristics. They are supervised by competent
scientists who are trained and experienced in working with these agents.
Access to the laboratory is strictly controlled by the laboratory director. The
facility is either in a separate building or in a controlled area within a building,
which is completely isolated from all other areas of the building. A specific
facility operations manual is prepared or adopted. A Class III biohazard
safety cabinet is required for work involving these agents.
From: Biosafety in Microbiology and Biomedical Laboratories, U.S. Department of
Health and Human Services, HHS publication (CDC) 99-8395, 4th ed. April 1999. p 11-14.
3
4
4
Level 3 plus:
• Clothing change before entry
into lab
• Shower on exit
• All material decontaminated
on exit from lab
Level 2 plus:
• Controlled access
• Decontamination of waste
• Decontamination of lab
clothing
• Baseline serum samples of
lab personnel
Level 1 plus:
• Limited access
• Biohazard warning signs
• “Sharps” precautions
• Biosafety manual
defining any needed waste
decontamination or medical
surveillance policies
• Respiratory protection as
required
Standard
Microbiological
practices
Practices and Techniques
Primary barriers:
All procedures conducted in Class III
or I or II BSCs in combination with
full-body, air-supplied, positivepressure personnel suit
Personal protective equipment:
lab clothing, gloves, respiratory
protection as needed
Primary barriers:
Class I or II BSCs or other physical
containment devices used for all open
manipulations of agents.
Personal protective equipment:
lab coats,gloves, face protection as
needed
Primary barriers:
Class I or II BSCs or other physical
containment devices used for all
manipulations of agents that cause
splashes or aerosols of infectious
materials.
None Required
Safety Equipment
(Primary Barriers)
Level 3 plus:
• Separate building or
isolated zone
• Dedicated supply/
exhaust vacuum,
and decontamination
systems
• Additional
requirements as
outlined in Biosafety
in Microbiological and
Biomedical Laboratories
Level 2 plus:
• Physical separation
from access corridors
• Self-closing, double
door access
• Exhaust air not
recirculated
• Negative airflow into
laboratory
Level 1 plus:
• Autoclave available
Open benchtop
sink required.
Facilities
(Secondary Barriers)
Source: Biosafety in Microbiology and Biomedical Laboratories,U.S. Department of Health and Human Services, HHS publication (CDC) 99-8395, 4th ed. p 52-53.
Ebola zaire, Sin
Nombre Virus, Rift
Valley Fever
Dangerous / exotic
agents that pose
a high risk of life
threatening disease.
Aerosol-transmitted
lab infections or
related agents with
uknown risk of
transmission
3
2
Measles virus,
salmonellae,
Toxoplasma spp,
Hepatitis B Virus.
M. Tuberculosis,
St. louis
encephalitis virus,
Coxiella Burnetii,
Bacillus anthracis
(production level)
Associated with
human disease.
Primary hazards are
percutaneous injury,
ingestion, mucous
membrane exposure
Bacillus subtilis,
Naegleria gruberi,
Infectious canine,
hepatitis virus,
E.coli
Examples
Table 1. Summary of Biosafety Levels and Infections Agents
Indigenous or
exotic agents with
potential for aerosol
transmission,
disease may have
serious or lethal
consequences
Not known to
consistently cause
disease in healthy
adults
Infectious Agents
1
Biosafety
Level
A Guide To Biosafety And Biosafety Cabinets
A Guide To Biosafety And Biosafety Cabinets
II. Biological Safety Cabinets
The terms biological safety cabinet and biosafety cabinet have been widely
used to describe a variety of containment devices equipped with HEPA filter(s),
designed to provide personnel or both personnel and product protection from
biohazardous materials. The terms should only be applied to those devices
that meet the requirements of Class I, II, or III specifications, based on their
construction, airflow velocities and patterns, and their exhaust systems.
Major International Standards for Biosafety Cabinets :

American Standard NSF 49:2002

European Standard EN 12469:2000

Australian Standard AS 2252

Japanese Standard JIS K 3800:2000
Table 2. Biohazard Safety Cabinet Classes
Class
Inflow
V (m/s)
Recycle
Air (%)
Exhaust
Air (%)
Control Plenum
Surrounded by
Exhaust
Alternatives
Biosafety
Level
I
US:0.38
EN:0.70
0
100
Outside Air
Inside room /
Hard duct
1, 2 & 3
II A1
US:0.38
EN:0.40
70
30
Outside Air
Inside room /
Thimble duct
1, 2 & 3
II A2
US:0.50
EN:0.40
70
30
Negative Plenum
Inside room /
Thimble duct
1, 2 & 3
II B1
US:0.50
EN:0.40
30
70
Negative Plenum
Hard duct
only
1, 2 & 3
II B2
US:0.50
EN:NA
0
100
Negative Plenum
Hard duct
only
1, 2 & 3
III
Closed:
>50”WC
0
100
Negative Plenum
Inside room /
Hard duct
1, 2, 3 & 4
Source: Laboratory Biosafety Manual. Second Edition (revised). Interim guidelines. World Health Organization. p32.
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A Guide To Biosafety And Biosafety Cabinets
Class I Biohazard Safety Cabinet
The Class I cabinet has the most basic and rudimentary design of all biohazard
safety cabinetry available today. A stream of inward air moving into the
cabinet contains aerosols generated during microbiological manipulations.
It then passes through a filtration system that traps all airborne particles
and contaminants. Finally, clean, decontaminated air is exhausted from the
cabinet. The filtration system usually consists of a pre-filter and a HEPA
(High Efficiency Particulate Air) filter.
Although the Class I cabinet protects the operator and the environment from
exposure to biohazards, it does not prevent samples being handled in the
cabinet from coming into contact with airborne contaminants that may be
present in room air. Naturally, there is a possibility of cross-contamination that
may affect experimental consistency. Consequently the scope and application
of Class I cabinets is limited and it is largely considered obsolete.
All Class I biohazard safety cabinets are suitable for work with microbiological
agents assigned to biological safety levels 1, 2 and 3.
Class II Biohazard Safety Cabinet
Like Class I safety cabinets, Class II cabinets have a stream of inward air
moving into the cabinet. This is known as the inflow and it prevents the
aerosol generated during microbiological manipulations to escape through
the front opening. However, unlike Class I cabinets, the inflow on Class II
cabinets flows through the front inlet grille, near the operator. None of the
unfiltered inflow air enters the work zone of the cabinet, so the product
inside the work zone is not contaminated by the outside air.
A feature unique to Class II cabinets is a vertical laminar (unidirectional) HEPAfiltered air stream that descends downward from the interior of the cabinet.
This continuously flushes the cabinet interior of airborne contaminants and
protects samples being handled within the cabinet from contamination and
is known as the downflow.
The differences between the various Class II cabinets available lie primarily
with the percentage of air exhausted to that of air re-circulated from the
common air plenum. In addition, different Class II cabinets have different
means of cabinet exhaust. Some cabinets may exhaust air directly back to
the laboratory, while others may exhaust air through a dedicated ductwork
system to the external environment.
Despite these differences, all Class II cabinets, like Class I cabinets, protect
both the operator and environment from exposure to biohazards. In addition,
Class II cabinets also protect product samples from contamination during
microbiological manipulations within the cabinet interior and are all suitable
for work with agents assigned to biological safety levels 1, 2 and 3.
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A Guide To Biosafety And Biosafety Cabinets
Class II Type A (A1/A2) Biohazard Safety Cabinet
The Class II Type A biohazard safety cabinet is the most common Class II
cabinet. It is also the most common safety cabinet of all the different types
available. It has a common plenum from which 30% of air is exhausted, and
70% re-circulated to the work area as the downflow.
Type A cabinets exhaust air directly back to the laboratory, and they may
contain positive pressure contaminated plenums. When toxic chemicals must
be employed as an adjunct to microbiological processes, these cabinets
should not be used. Exhaust HEPA filtration only removes airborne aerosols
including biohazards, and not chemical fumes.
The Class II Type A1 has the positively-pressurized contaminated plenum
bordering the ambient environment, and therefore is less safe than the
Class II Type A2 that has a negative pressure surrounding the positively
pressurized contaminated plenum. In case there is a leakage on the positive
plenum, the leaking aerosol will be pulled by the negative pressure back to
the positive plenum, and it wont leak outside. Because of the safety issue,
the Type A1 design is now considered obsolete. In the A2 cabinet, about
70% of air from the positive plenum is re-circulated as downflow, and the
remaining 30% is discharged to the lab through the exhaust filter.
Class II Type B Biohazard Safety Cabinets
The main difference between Type A and Type B cabinet is: Type B cabinets
must be operated with an external blower and it exhausts air to the external
environment via a dedicated ductwork system. Without the external blower,
the cabinets internal blower will blow the air (and microbiological agents)
inside the work zone through the front operator, towards the operators face,
creating a dangerous situation. This cabinet is not self-balancing in the sense
that its own blower can only create downflow and the cabinet relies on the
external blower to create inflow.
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A Guide To Biosafety And Biosafety Cabinets
On all Type B cabinets, environmental protection may be enhanced by
installing a scrubbing system between the exhaust of the biohazard and
the final exhaust point outside the building to neutralize the chemical fumes
present in exhaust air.
Although Type B cabinets are commonly used when chemicals are involved in
your work processes, they theoretically provide an increased level of safety
as compared to other Type A cabinets. By exhausting air directly to the
external environment, they provide an additional “fail-safe” in the event that
the regular exhaust HEPA filtration ceases to function.
Class II Type B1 Biohazard Safety Cabinet
The Class II Type B1 biohazard safety cabinet was originally specified by the
American National Cancer Institute. It has a common plenum from which
70% of air is exhausted, and 30% re-circulated to the work area as the
downflow.
Type B1 cabinets also have a dedicated exhaust feature that eliminates
re-circulation when work is performed towards the back within the interior
of the cabinet. Toxic chemicals employed as an adjunct to microbiological
processes should only be employed if they do not interfere with work when
re-circulated in the downflow.
Class II Type B2 Biohazard Safety Cabinet
In the Class II Type B2 cabinet all inflow and downflow air is exhausted after
HEPA filtration to the external environment without recirculation within the
cabinet. Type B2 cabinets are suitable for work with toxic chemicals employed
as an adjunct to microbiological processes under all circumstances since no
re-circulation occurs. In theory, Type B2 cabinets may be considered to be
the safest of all Class II biohazard safety cabinets since the total exhaust
feature acts as a fail-safe in the event that the downflow and / or exhaust
HEPA filtration systems cease to function normally.
Fig 3. Class II Type B2
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A Guide To Biosafety And Biosafety Cabinets
Class III Biohazard Safety Cabinet
The Class III biohazard safety cabinet provides an absolute level of safety,
which cannot be attained with Class I and Class II cabinets. All Class III
cabinets are usually of welded metal construction and are designed to
be gastight. Work is performed through glove ports in the front of the
cabinet. During routine operation, negative pressure relative to the ambient
environment is maintained within the cabinet. This provides an additional
fail-safe mechanism in case physical containment is compromised.
On all Class III cabinets, a supply of HEPA filtered air provides product
protection and prevents cross contamination of samples. Exhaust air is
usually HEPA filtered and incinerated. Alternatively, double HEPA filtration
with two filters in series may be utilized. Materials are transferred into the
cabinet using a pass-through unit installed at the side of the work area. Class
III cabinets usually exhaust air back to the laboratory; however, air may also
be exhausted via a dedicated ductwork system to the external environment.
When a dedicated ductwork system is employed, they are also suitable for
work employing toxic chemicals as an adjunct to microbiological processes.
All Class III biohazard safety cabinets are suitable for work with microbiological
agents assigned to biological safety levels 1, 2, 3 and 4. They are frequently
specified for work involving the most lethal biological hazards.
Fig 4. Class III BSC
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A Guide To Biosafety And Biosafety Cabinets
HEPA & ULPA Filter
The HEPA filter is the heart of the biosafety cabinet. The HEPA filter is a
disposable dry-type filter, constructed of boron silicate microfibers cast into
a thin sheet, much like a piece of paper


HEPA: High Efficiency Particulate Air: Modern “American-convention”
HEPA: 99.99% at 0.3 microns
ULPA: Ultra Low Penetration Air: Modern “American-convention” ULPA:
99.999% at 0.12 microns
HEPA / ULPA filter construction:
Fig 5. Pack is glued into a frame - Frame is gasketed to form final assembly
Fig 6. Separator Type
Fig 7. Separatorless Type
HEPA/ULPA filters are designed to remove a broad range of airborne
contaminants, including:
 Fine dust
 Smoke
 Bacteria (typical size: 500 to 0.3 micron)
 Soot
 Pollen
 Radioactive particles
 Impurity ion -> can affect Integrated Circuit speed
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A Guide To Biosafety And Biosafety Cabinets
III. Proper Selection, Installation & Safe Use Of BSCs
A. Selection of A Biological Safety Cabinet
A BSC should be selected primarily in accordance with the type of protection
needed: product protection; personnel protection against Risk Group 1–4
microorganisms; personnel protection against exposure to radionuclides and
volatile toxic chemicals; or a combination of these. Table 3 shows which
BSCs are recommended for each type of protection.
Table 3. Biohazard Safety Cabinet Selection
Type of protection
BSC Selection
Personnel Protection, microorganisms in Risk
Groups 1-3
Class I, Class II, Class III
Personnel Protection, microorganisms in Risk
Groups 4, glove box laboratory
Class III
Personnel Protection, microorganisms in Risk
Groups 4, suit laboratory
Class I, Class II
Product Protection
Class II, Class III only if laminar
flow included
Volatile radionuclide / chemical protection,
minute amounts
Class IIB1, Class IIA2 vented to the
outside
Volatile radionuclide / chemical protection
Class I, Class IIB2, Class III
Source: Laboratory Biosafety Manual. Second Edition (revised). Interim guidelines.
World Health Organization. p29.
Volatile or toxic chemicals should not be used in BSCs that recirculate exhaust
air to the room, i.e. Class I that are not ducted to building exhaust systems,
or Class IIA1 or Class IIA2 cabinets. Class II B1 cabinets are acceptable
for work with minute amounts of volatile chemicals and radionuclides. A
Class IIB2 cabinet, also called a total exhaust cabinet, is necessary when
significant amounts of radionuclides and volatile chemicals are expected to
be used.
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A Guide To Biosafety And Biosafety Cabinets
B. Using Biological Safety Cabinets in The Laboratory
1. Location
The velocity of air flowing through the front opening into a BSC is about 0.45
m/s. At this velocity the integrity of the directional air inflow is fragile and
can be easily disrupted by air currents generated by people walking close
to the BSC, open windows, air supply registers, and opening and shutting
doors.
Ideally, BSCs should be situated in a location remote from traffic and
potentially disturbing air currents. Whenever possible a 30 cm clearance
should be provided behind and on each side of the cabinet to allow easy
access for maintenance. A clearance of 30–35 cm above the cabinet may
be required to provide for accurate air velocity measurement across the
exhaust filter and for exhaust filter changes.
2. Operators
If BSCs are not used properly, their protective benefits may be greatly
diminished. Operators need to be careful to maintain the integrity of the front
opening air inflow when moving their arms into and out of cabinets. Arms
should be moved in and out slowly, perpendicular to the front opening.
Manipulations of materials within BSCs should be delayed for about 1 min
after placing hands and arms inside to allow the cabinet to adjust and to
“air sweep” the surface of the hands and arms. The number of movements
across the front opening should also be minimized by placing all necessary
items into the cabinet before beginning manipulations.
BSC

BSC
Biological Safety Cabinet
Return Air Grille
Personnel Traffic
Barrier Airflow
BSC
BSC

BSC


BSC
Air Inlet

Fig 8. A typical Class II Cabinet Installation
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
A Guide To Biosafety And Biosafety Cabinets
3. Material Placement
The front intake grill of Class II BSCs must not be blocked with paper,
equipment or other items. Materials to be placed inside the cabinet should
be surface-decontaminated with 70% alcohol. Work may be performed on
disinfectant-soaked absorbent towels to capture splatters and splashes.
All materials should be placed as far back in the cabinet, towards the rear
edge of the work surface, as practical without blocking the rear grill. Aerosolgenerating equipment (e.g. mixers, centrifuges, etc.) should be placed
towards the rear of the cabinet. Bulky items, such as biohazard bags, discard
pipette trays and suction collection flasks should be placed to one side of the
interior of the cabinet. Active work should flow from clean to contaminated
areas across the work surface.
The autoclavable biohazard collection bag and pipette collection tray should
not be placed outside the cabinet. The frequent in-and-out movement
needed to use these containers is disruptive to the integrity of the cabinet’s
air barrier, and can compromise both personnel and product protection.
4. Operation and Maintenance
Most BSCs are designed to permit operation 24 h/day, and investigators find
that continuous operation helps to control the levels of dust and particulate
materials in the laboratory. Class IIA1 and IIA2 BSCs exhausting to the
room or connected by thimble connections to dedicated exhaust ducts can
be turned off when not in use. Other types such as IIB1 and IIB2 BSCs,
which have hard-duct installations, must have air flow through them at all
times to help maintain room air balance.
Cabinets should be turned on at least 5 min before beginning work and after
completion of work to allow the cabinet to “purge”, i.e. to allow time for
contaminated air to be removed from the cabinet environment.
All repairs made on BSCs should be made by a qualified technician. Any
malfunction in the operation of the BSC should be reported and repaired
before the BSC is used again.
5. Ultraviolet Lights
Ultraviolet lights are not required in BSCs. If they are used, they must be
cleaned weekly to remove any dust and dirt that may block the germicidal
effectiveness of the light. Ultraviolet light intensity should be checked
when the cabinet is recertified to ensure that light emission is appropriate.
Ultraviolet lights must be turned off while the room is occupied, to protect
eyes and skin from inadvertent exposure.
6. Open Flames
Open flames should be avoided in the near microbe-free environment created
inside the BSC. They disrupt the air flow patterns and can be dangerous when
volatile, flammable substances are also used. To sterilize bacteriological
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A Guide To Biosafety And Biosafety Cabinets
loops, micro-burners or electric “furnaces” are available and are preferable
to open flames.
7. Spills
A copy of the laboratory’s protocol for handling spills should be posted,
read and understood by everyone who uses the laboratory. When a spill of
biohazardous material occurs within a BSC, cleanup should begin immediately,
while the cabinet continues to operate. An effective disinfectant should be
used and applied in a manner that minimizes the generation of aerosols. All
materials that come into contact with the spilled agent should be disinfected
and / or autoclaved.
8. Annual Certification
The functional operation and integrity of each BSC should be certified to
national or international performance standards at the time of installation and
regularly thereafter by qualified technicians, according to the manufacturer’s
instructions.
Evaluation of the effectiveness of cabinet containment should include tests
for cabinet integrity, HEPA filter leaks, downflow velocity profile, face velocity,
negative pressure/ventilation rate, air flow smoke pattern, and alarms and
interlocks.
Optional tests for electrical leaks, lighting intensity, ultraviolet light intensity,
noise level and vibration may also be conducted. Special training, skills and
equipment are required to perform these tests and it is highly recommended
that they are undertaken by a qualified professional.
9. Cleaning and Disinfection
All items within BSCs, including equipment, should be surface-decontaminated
and removed from the cabinet when work is completed, since residual
culture media may provide an opportunity for microbial growth. The interior
surfaces of BSCs should be decontaminated before and after each use. The
work surfaces and interior walls should be wiped with a disinfectant that will
kill any microorganisms that might be found inside the cabinet. At the end of
the work day, the final surface decontamination should include a wipe-down
of the work surface, the sides, back and interior of the glass. A solution of
bleach or 70% alcohol should be used where effective for target organisms.
A second wiping with sterile water is needed when a corrosive disinfectant,
such as bleach, is used. It is recommended that the cabinet be left running.
If not, it should be run for 5 min in order to purge the atmosphere inside
before it is switched off.
10. Decontamination
BSCs must be decontaminated before filter changes and before being
moved. The most common decontamination method is by fumigation with
formaldehyde gas. BSC decontamination should be performed by a qualified
professional.
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A Guide To Biosafety And Biosafety Cabinets
11. Personal protective equipment
Personal protective clothing should be worn whenever using a BSC. Laboratory
coats are acceptable for work being performed at Biosafety Levels 1 and 2.
A solid front, back-closing laboratory gown provides better protection and
should be used at Biosafety Levels 3 and 4 (except for suit laboratories).
Gloves should be pulled over the wrists of the gown rather than worn inside.
Elasticized sleeves can be worn to protect the investigator’s wrists. Masks
and safety glasses may be required for some procedures.
12. Alarms
BSCs can be equipped with one of two kinds of alarm. Sash alarms are found
only on cabinets with sliding sashes. The alarm signifies that the operator
has moved the sash to an improper position. Corrective action for this type
of alarm is returning the sash to the proper position. Air-flow alarms indicate
a disruption in the cabinet’s normal air-flow pattern. This represents an
immediate danger to the operator or product. When an air-flow alarm sounds,
work should cease immediately and the laboratory supervisor should be
notified. Manufacturers’ instruction manuals should provide further details.
Training in the use of BSCs should cover this aspect.
13. Supplementary Information
Selecting the correct type of BSC, installing it, using it properly and annually
certifying its operation are complex processes. It is highly recommended
that they proceed under the supervision of a well-trained and experienced
biosafety professional. The professional should be highly familiar with the
relevant literature listed in the References section, and should have been
trained on all aspects of BSCs. Operators should receive formal training in
the operation and use of BSCs.
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A Guide To Biosafety And Biosafety Cabinets
C. Safe Use of Biosafety Cabinets
1. Using BSCs: Startup
 Wear gloves for hand protection
 Load the cabinet with all items required prior to starting work and surface
decontaminate
 Surface decontaminate the work surface, side walls and inner back walls
 Allow the work zone air to purge for a few minutes before commencing
work
 Do not overcrowd the work zone
 Close the drain valve before operation
2. Using BSCs: Operation
 Do not obstruct the front or back air grilles
 Work as far in to the cabinet as possible
 Minimize arm movement; make slow movements to avoid disrupting
cabinet airflow
 When removing arms from cabinet be sure to surface decontaminate first,
and move arms out slowly of the cabinet (in direction perpendicular to
plane of work zone opening)
 Minimize external airflow disturbances
 Work from “clean to dirty”
 Biohazard collection bags should be placed inside the cabinet instead of
outside
 Use absorbent pads on the work surface where appropriate to minimize
splatter and aerosol generation in case of a spillage
 Surface decontaminate before removing potentially contaminated items
from the interior
 Place aerosol-generating instruments as far in to the interior of the cabinet
as possible
 Clean materials should be at least 150mm away from aerosol generating
objects to minimize the chance for cross contamination
 Hold lids / covers above dishes / sample plates in order to prevent
impingement of downward air
 Do not use a gas flame whenever possible as it interferes with airflow
 Put air turbulence generating equipment such as centrifuge, blender, or
sonicator in the back 1/3 of the cabinet
3. Bunsen Burner
Burners can cause an outflow of air from inside the cabinet that can endanger
the operator. On Class II cabinets, protection against cross contamination
may also be compromised. There have been incidents where the sash of a
recirculating Class II safety cabinet was closed while the burner was still on,
causing heat build up in the cabinet that damaged the filters.
Bunsen Burner Safety:
1. An electronic burner which automatically reduces the gas supply to
maintain a smaller flame during periods of non-use
2. An emergency shut off valve should be easily accessible near to the
cabinet in case of an emergency.
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A Guide To Biosafety And Biosafety Cabinets
3. The burner should be placed as far into the cabinet working space as
possible to minimize its effect on cabinet performance.
4. A solenoid valve can be interlocked with the gas supply to automatically
shut off the burner
4. Using BSCs: Shutdown
Note: operate the cabinet continuously whenever possible to maximize
protection- Surface decontaminate all items
 Seal biohazard bags if used
 Surface decontaminate the cabinet innerside walls, back wall, work surface,
drain pan and the inner side of the sliding sash / hinged window
 Allow work zone air to purge
 When available: install front closure or close sash and activate UV lamp
(if desired)
5. User maintenance
 Pre-filter replacements once every 3 months
 UV lamp replacement once a year
 Operational verification if appropriate (e.g. Alarm checks, basic airflow
measurements etc.)
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A Guide To Biosafety And Biosafety Cabinets
IV. Biohazard Spills & Cleanup Procedures
A. Inside the BSC:
 Wait at least five minutes to allow the BSC to contain aerosols.
 Wear lab coat, safety glasses and gloves during cleanup.
 Allow cabinet to run during cleanup.
 Apply disinfectant and allow a minimum of 20 minutes contact time.
 Wipe up spillage with disposable disinfectant-soaked paper towel.
 Wipe the walls, work surface and any equipment in the cabinet with a
disinfectant-soaked paper towel.
 Discard contaminated disposable materials using appropriate biohazardous
waste disposal procedures (e.g. autoclave or BFI).
 Place contaminated reusable items in biohazard bags, autoclavable pans
with lids or wrap in newspaper before autoclaving and cleanup.
 Expose non-autoclavable materials to disinfectant (20 minute contact
time) before removal from the BSC.
 Remove protective clothing used during cleanup and place in a biohazard
bag for autoclaving.
 Run cabinet 10 minutes after cleanup before resuming work or turning
cabinet off.
B. Inside the lab, outside the BSC:
 Call the biosafety office if the material is BSL-2 or greater containment.
 Clear area of all personnel. Wait at least 15 minutes for aerosol to settle
before entering spill area.
 Remove any contaminated clothing and place in biohazard bag to be
autoclaved.
 Put on a disposable gown, safety glasses and gloves.
Initiate cleanup with disinfectant as follows:
1. Place dry paper towel on spill (to absorb liquids); then layer a second set
of disinfectant soaked paper towels over the spill.
2. Encircle the spill with additional disinfectant being careful to minimize
aerosolization while assuring adequate contact.
3. Decontaminate all items within spill the area.
4. Allow 20 minutes contact time to ensure germicidal action of
disinfectant.
5. Wipe equipment with appropriate disinfectant.
6. Discard contaminated disposable materials using appropriate biohazardous
waste disposal procedures (e.g., autoclave or BFI).
7. Disinfect reusable items
C. Inside The Centrifuge
 Clear area of all personnel.
 Wait 30 minutes for aerosol to settle before attempting to clean up spill.
 Wear a lab coat, safety glasses and gloves during cleanup.
 Remove rotors and buckets to nearest biological safety cabinet for
cleanup.
 Thoroughly disinfect inside of centrifuge.
 Discard contaminated disposable materials using appropriate biohazardous
waste disposal procedures (e.g., autoclave or BFI).
18
A Guide To Biosafety And Biosafety Cabinets
D. Outside The Lab, In Transit
 Transport labelled biohazardous material in an unbreakable, well-sealed
primary container placed inside of a second unbreakable, lidded container
(cooler, plastic pan or pail) labelled with the biohazard symbol.
 Should a spill occur in a public area, do not attempt to clean it up without
appropriate personal protective equipment.
 Secure the area, keeping all people well clear of the spill.
 Call professional help to assist in cleanup.
 Stand by during spill response and cleanup activity and provide assistance
only as requested or as necessary.
From: UCSD Biosafety handbook, UCSD EH&S Biosafety Team 1996.
THE UNIVERSITY OF CALIFORNIA, USA. p95-97.
V. References:
1.
2.
3.
4.
5.
User & Service Manual. Labculture® Class II Type A2 Biohazard
Safety Cabinet: ESCO Micro Pte Ltd.
Biosafety in Microbiological and Biomedical Laboratories
(BMBL). 4th Edition. Office of Health and Safety (OHS), Centers for
Disease Control and Prevention (CDC). US Department of Health.
Laboratory Biosafety Manual. Second Edition (revised). Interim
guidelines. World Health Organization.
UCSD Biosafety handbook, UCSD EH&S Research Community for
Biosafety. 1996.
UCSD Biosafety handbook, UCSD EH&S Biosafety Team 1996. THE
UNIVERSITY OF CALIFORNIA.
19
PRODUCTS AVAILABLE FROM ESCO BIOTECH:
Vertical Laminar Flow Cabinets
Horizontal Laminar Flow Cabinets
PCR Vertical Laminar Flow Cabinets
Class II Biosafety Cabinets
Class III Biosafety Cabinets
Cytotoxic Cabinets
IVF Cabinets
Weighing Cabinets
Animal Handling Workstations
Custom-Made Clean-Air / Containment Workstations
Your local distributor:
Esco Biotechnology Equipment Division
21 Changi South Street 1
Singapore 486777
tel: +65 6542 0833 fax: +65 6542 6920
email: [email protected]
website: biotech.escoglobal.com
©2005 Esco Micro Pte. Ltd.
Airstream Class II, Biological Safety Cabinet (E-series),
Model AC2-4E_
Class II, Biological Safety Cabinets
The Safety Solution for Life Science Laboratories
Main Features
Unique Esco Dynamic Chamber™ plenum design delivers quiet, uniform airflow.
Negative pressure plenum surrounds contaminated
positive pressure plenum; no fabric bags are used.
Dual, long-life ULPA filters for supply and exhaust airflow.
Esco Sentinel™ microprocessor supervises all cabinet
functions.
Ergonomically angled front improves reach and
comfort.
Actual work opening is 25.4 mm (1") larger than tested
sash opening to provide additional work space.
Frameless, shatterproof sash is easier to clean, offers larger, unobstructed viewing area.
Removable one-piece work surface simplifies cleaning
(applicable to AC2-S series and AC2-D series).
E-Series cabinets include multi-piece tray components
which lift and remove to provide easy access encourage
surface decontamination and can be sterilized inside
autoclave.
Raised airflow grille maintains safety by preventing
blockage.
Airstream E-Series Class II Biological
Safety Cabinet features glass sides to
enhance visibility inside the work area.
Model AC2-4E_.
Airstream AC2 Series Model Number, Side Wall Configuration
Glass, E-Series
Stainless Steel
S-Series
Stainless Steel,
DUO, D-Series
0.6 meters (2')
AC2-2E_
NIL
NIL
0.9 meters (3')
AC2-3E_
AC2-3S_
NIL
1.2 meters (4')
AC2-4E_
AC2-4S_
AC2-4D_
1.5 meters (5')
AC2-5E_
AC2-5S_
NIL
1.8 meters (6')
AC2-6E_
AC2-6S_
AC2-6D_
Cabinet Size
Airstream S-Series Class II
Biological Safety Cabinet
with stainless steel side,
Model AC2-4S_.
Note: The last digit of the Model Number _ indicates desired
electrical service (60Hz, 50Hz). See the Specification Chart for
details. Specify voltage/Hz when ordering.
Biological Safety Cabinet • Class II Microbiological Safety Cabinets (S-Series, E-Series and Airstream Duo)
Improved lighting is brighter, more
uniform, reduces glare.
Optional UV lamp is located behind
control panel away from line of sight;
lamp operates on programmable timer.
Esco
antimicrobial surface
on all painted surfaces minimizes
contamination.
Integrated RFI and electrical noise filters
eliminate interference with and from
adjacent equipment.
E-Series with Tempered Glass Side Walls
• Multi-piece work surface removal simplifies cleaning.
• Glass sides increase visibility and prevent the operator from being "boxed-in."
S-Series with Stainless Steel Side Models
• Enhanced side-capture zones and negative pressure side walls optimize containment.
• Interior work area formed from a single piece of stainless steel with large radius corners to simplify cleaning.
D-Series Duo, with Stainless Steel Sides, Dual Blower Motors, Dual Exhaust Filters
Airstream Duo, Class II Biological Safety
Cabinet with stainless steel side, dual
blower motors and dual exhaust filter,
Model AC2-4D_.
• Dual fan design for redundant operation.
• Dual exhaust filters for added safety.
• If one fan fails, the second fan provides minimum containment.
• ULPA filters (per IEST-RP-CC001.3),
are tested to a typical efficiency of
>99.999% for 0.1 to 0.3 micron
particles; these provide better filtration
capability than conventional H13 HEPA
filters that have a typical efficiency of
> 99.99% for 0.3 micron particles.
Containment and Protection
• A combination of a supply ULPA filter
and an exhaust ULPA filter create a fully
integrated performance envelope for
product, operator and environmental
protection.
• Inflow of room air enters the front air
grille to establish operator protection;
room air does not enter the work zone,
preventing product contamination.
• Modern separatorless mini-pleat filter
construction maximizes the filter surface
area to extend filter life and eliminate
possible filter media damage by thin
and sharp aluminum separators used in
conventional HEPA filter construction.
• Raised armrest prevents the likelihood
of inflow grille blocking by operator’s
arms.
• Auto-purge holes located at the front
side walls (on AC2-S Series) eliminate
eddy currents and dead air pockets
in the critical area behind the sash
window.
• The inflow velocity, downflow velocity,
air flow path and intake geometry
are precision tuned and tested to
create an optimum air curtain on the
front aperture; this curtain maintains
personal and product protection
even in the unlikely event of a severe
inflow or downflow imbalance that
would compromise protection in a
conventional cabinet.
Integrated Filtration System
Independent supply and exhaust filters
provide 99.999% typical efficiency for
• The filter assembly is constructed in
accordance with EN1822 requirements.
Operator, Product and Environmental Protection
Esco Airstream Class II Biological Safety Cabinets offer a
premium level of operator, product and environmental
protection with advanced technology at an economical
price. Intelligent, ergonomic design enhances productivity,
operator comfort, maintenance and utility value. With
an extensive track record of safety, reliability and
performance, Airstream cabinets make ideal investments
for a wide range of general laboratory applications.
Airstream biological safety cabinets provide protection
against Biosafety Levels 1, 2 and 3,and can be used for
handling Biosafety Level 4, provided that the operator
wears a positive pressure suit.
particle sizes of 0.1 to 0.3 microns.
Airstream filters meet the IEST-RPCC001.3 recommended practice for ULPA
performance (USA), and EN 1822 for H14
performance (EU).
• The supply filter provides ISO Class 3
(per ISO14644.1) clean air to the work
surface in a gentle vertical laminar flow
for product protection.
• The exhaust filter traps biohazard
particles acquired from the work surface
before air is exhausted to the room,
offering personal and environmental
protection.
• The Airstream Duo includes two exhaust
filters to double exhaust protection.
• The exhaust filter media is protected
from mechanical damage by an
integrated metal screen guard, which is
absent from conventional HEPA filters.
Mini-pleat Separatorless Filter (left) vs.
Conventional Aluminum Separator Filter (right)
drop down on the drain pan, preventing
them from being pulled into the column
by fan suction.
Dual Blower System, Airstream
D-Series Duo Models
The Airstream Duo includes a dual blower
system designed for redundancy.
Esco cabinets use Swedish Camfil Farr® mini-pleat
filters without aluminum separators to increase filter
efficiency, minimize the chance of leakage, and to
prolong filter life. Filters include a lightweight
aluminum frame for structural stability and
elimination of swelling common to conventional
wood frames.
Single Blower System, Airstream S-Series and E-Series Models
The AC2 blower system is designed for high
performance operation, maximum energy
efficiency and minimal maintenance.
• Industry exclusive backward curved,
motorized impeller design replaces
conventional blowers.
• Improved energy efficiency lowers
operating costs.
• Reduced noise and vibration levels
over conventional blowers provide a
comfortable working environment.
• An integral blower hour meter tracks
operating life and aids in predictive
maintenance planning.
• Built-in RFI and electrical noise filters
eliminate interference with adjacent
instrumentation.
• The external rotor motor design allows
for optimum cooling of the motor
during extended operations and
extends the motor bearing life.
• Speed can be adjusted electronically
without the need for mechanical
adjustment.
• To prevent fan damage, a paper-catch
grille traps papers or towels that may
• Dual permanently lubricated directdrive external rotor motor/blowers
assure cabinet safety in the event of a
motor failure.
• A true airflow velocity sensor provide
independent measurement of inflow
and downflow velocities despite room
temperature fluctuation.
• All electronic parts are contained inside
a plug-and-play module that permits
easy exchange if required.
• Microprocessor software updates are
available from Esco for download via
the Internet.
• The external rotor motor design allows
for optimum cooling of the motor
during extended operations.
Consult your Esco Operating Manual or
contact Esco or your Sales Representative
for information on user-preference
programming capabilities built into the
Sentinel microprocessor platform.
• The double blower system assures inherently safe operation if one blower fails.
Cabinet Construction
Front Sash Assembly
The front sash is counterbalanced for
convenient one-hand operation.
• The frameless sash eliminates operator’s
line of sight blockage.
• A generous sash opening allows for
easier access into the work zone,
provides ample room for transferring of
small equipment; see Specifications.
• The sliding window can be fully opened
to insert and remove larger instrumentation and equipment.
SentinelTM Microprocessor Control,
Alarm, Monitoring System
The Esco Sentinel™ microprocessor-based
control system supervises operation of all
cabinet functions.
• The control panel is located on the
center of the cabinet, and angled down
for easy access by the operator
• Continuous monitoring of cabinet
airflow is displayed on a bright, easyto-read LCD panel. The large display
monitors operational parameters.
Robust construction and enhanced safety
features qualify the cabinet for the most
demanding laboratory applications. The
cabinet is fully assembled and ready to
install and operate when shipped.
• The cabinet work zone has no welded
joints to collect contaminants or rust.
• All stainless steel work surfaces are
accessible for cleaning.
• S Series cabinets (stainless steel sides)
include interior sides and back wall
formed from a single piece of stainlesssteel with large radius corners to simplify
interior cleaning.
• E Series cabinets (tempered glass sides)
include multi-piece tray components
which lift and remove to provide
easy access and to encourage surface
decontamination.
• A recessed central area and drain pan
channels spills and prevent liquids from
entering the lower filtration and blower
systems.
• The drain pan is flush with the side walls
to eliminate concealed or hard-to-clean
spaces.
• There are no screws on the front or sides
to trap contaminants or complicate
cleaning.
Esco ULPA Filter Efficiency
Independent supply and exhaust
filters provide 99.999% typical
efficiency for particle sizes of 0.1
to 0.3 microns. Airstream Series
filters meet the IEST-RP-CC001.3
recommended practice for ULPA
performance (USA), and EN 1822
for HEPA performance (EU).
Typical Penetration
• External surfaces are coated with Esco
Isocide ™ antimicrobial coating to
protect against surface contamination
and inhibit bacterial growth. Isocide
eliminates 99.9% of surface bacteria
within 24 hours of exposure.
Service Fitting Access
The cabinet is prepared for easy installation
of optional gas and vacuum fittings; see
Accessories.
Biological Safety Cabinet • Class II Microbiological Safety Cabinets (E-Series, S-Series and D-Series)
Touchpad data entry buttons permit
control settings and access to diagnostics,
default settings and hierarchical menus.
Color coded indicator lamps display green
for primary function (fan operation); blue
for secondary function (fluorescent lights
and electrical outlet); and orange for
caution (UV lamp ON).
Sentinel Microprocessor Control System, Programmable
• Optional service fittings openings are
offset for easier access.
Comfortable Ergonomic Design
Programmable automatic UV light
timer simplifies operation, enhances
contamination control, extends UV
lamp life and saves energy.
A graphical interface indicates cabinet performance.
Digital read-out with alpha-numeric display indicates
all input, status and alarm functions.
All functions can be user activated through touch-pad
programming access; see Operations Manual.
When programmed ON • the start-up sequence confirms status with Air Safe and local time display.
• the Personal Identification Number (PIN) access restricts unauthorized adjustments.
• an airflow alarm warns of deviations from normal velocities.
Electrical Safety and Certification
All components meet or exceed applicable
safety requirements.
Support Stands
• Fixed height, available 737 mm (29")
or 838 mm (33"), ±38.1 mm (1.5")
- With leveling feet
- With casters
The cabinet is engineered for comfort,
utility value and safety.
• Each cabinet is individually factory
tested for electrical safety.
• The angled viewing window and narrow
profile front grille improves reach into
the work area.
• Documentation specific to each cabinet
serial number is maintained on file.
• Adjustable height, hydraulic range
737 mm (29") to 838 mm (33")
- With casters
• Certified to major world standards
for microbiological safety cabinets,
including the EN12469 (refer to technical
specifications for the full list).
• Telescoping height stand, nominal
range 737 mm (29") or 838 mm (33")
- Adjustable in 25.4 mm (1") increments
• The instant-start 5000k fluorescent
lamp operates on an electronic ballast
to reduce heat, improve comfort and
conserve energy.
• The lamp delivers uniform lighting to
the work surface for greater comfort,
reduced glare and improved productivity;
see Specifications.
• The front armrest is raised above the
work zone to improve comfort and to
minimize blockage of forward airflow
perforations.
• The optional adjustable support stand
provides work surface height control.
• The frameless sash eliminates operator’s
line of sight blockage.
• A generous sash opening allows for
easier access into the work zone, provides ample room for transferring of
small equipment; see Specifications.
• The sliding window can be fully
opened to insert and remove larger
instrumentation and equipment.
• Contact Esco or your Sales Representative
for site preparation information; see
Electrical Specifications.
Warranty
Airstream AC2 Series cabinets are warranted for 3 years excluding consumable
parts and accessories.
• Each cabinet is shipped with a comprehensive User’s Manual complete
with a report documenting all test
procedures.
• Additional IQ/OQ/PQ documentation
is available upon request.
• Contact your local Sales Representative
for specific warranty details or documentation requests.
Accessories and Options
Esco offers a variety of options and
accessories to meet local applications.
Contact Esco or your local Sales Representative for ordering information.
• Infinitely adjustable cradle stand, with casters
- Elevates to seating or standing work surface height - When lowered permits movement through standard doorway
Note: Increases exterior dimensions
Electrical Outlets and Utility Fixtures
• Electrical outlet, ground fault,
North America
• Electrical outlet, Europe / Worldwide
• Petcock (air, gas, vacuum)
-North America (American) style
-Europe / Worldwide style DIN 12898,
DIN 12919, DIN 3537
Cabinet Accessories
• Germicidal UV lamp
-Controlled by automatic UV lamp
TM
timer through Sentinel micro-
processor control panel
-Emission of 253.7 nanometers for
most efficient decontamination
Cabinet Filtration System
Stainless Steel Side Walls/ Tempered Glass
Side capture zones (applicable for AC2-S Series only)
Dynamic air barrier, inflow and forward-directed downflow air converge
Ambient air is pulled through the
perforations located towards the work
zone front to prevent contamination of
the work surface and work product. The
inflow does not mix with the clean air
within the cabinet work zone. Inflow air
travels through a return path toward the
common air plenum (blower plenum) at
the top of the cabinet.
A combination of inflow and downflow
air streams forms an air barrier that
prevents contaminated room air from
entering the work zone, and prevents
work surface emissions from escaping
the work zone.
Air returns to the common air plenum
where the 35% exhaust and 65%
recirculation process is continued.
The uniform, non-turbulent air stream
protects against cross contamination
within and throughout the work area.
ULPA-filtered air
Unfiltered / potentially contaminated air
Room air / Inflow air
Near the work surface, the downflow
air stream splits with a portion moving
toward the front air grille, and the
remainder moving to the rear air grille.
A small portion of the ULPA filtered
downflow enters the intake perforations
at the side capture zones at a higher
velocity (small blue arrows).
Airstream Model AC2 (E-Series) Biological Safety Cabinet Technical Specifications,
Stainless Steel Side Walls
1
2
1360 mm (52.9")
8
3
11
12
9
4
10
5
6
7
560 mm (22")
1035 / 1340 / 1645 / 1950 mm
(40.7" / 52.7" / 64.7" / 76.7")
1. Exhaust ULPA filter
5. Plugged service fixture provisions
(2 on each side)
740 mm (29.1")
3. Downflow ULPA filter
6. Stainless steel work zone
7. Electrical outlet Retrofit Kit provision 10. IV bar Retrofit Kit provision
(2 & 3 ft model - only 1 single outlet 11. Esco Sentinel microprocessor in work zone left side wall)
control system
4. Standard UV light Retrofit Kit Provision
8. Electrical panel
2. Blower
12. Sash window
9. Fluorescent lamp
Esco Centrifugal Fan with External Rotor Motor (left) vs.
Conventional Fan with Standard Motor (right)
Esco Airstream cabinets use a combination of high performance scroll blowers (supply) and German made
ebm-papst® permanently lubricated, centrifugal motor/blowers with external rotor designs (exhaust).
Selected for energy efficiency, compact design, and flat profile, the completely integrated exhaust blower
assembly optimizes motor cooling, with unified rotating parts and overall component balance for smooth,
quiet, vibration-free operation.
Weight is equally distributed to all bearings to extend bearing life, transfer heat and maximize speed control.
Biological Safety Cabinet • Class II Microbiological Safety Cabinets (E-Series, S-Series and D-Series)
Airstream Model AC2 (S-Series) Biological Safety Cabinet Technical Specifications,
Stainless Steel Side Walls
1
2
1360 mm (52.9")
8
3
11
9
12
10
4
5
6
7
560 mm (22")
1035 / 1340 / 1645 / 1950 mm
(40.7" / 52.7" / 64.7" / 76.7")
740 mm (29.1")
5. Plugged service fixture provisions
(2 on each side)
1. Exhaust ULPA filter
3. Downflow ULPA filter
6. Stainless steel work zone
7. Electrical outlet Retrofit Kit provision 10. IV bar Retrofit Kit provision
(2 & 3 ft model - only 1 single outlet 11. Esco Sentinel microprocessor in work zone left side wall)
control system
4. Standard UV light Retrofit Kit Provision
8. Electrical panel
2. Blower
12. Sash window
9. Fluorescent lamp
(cont.)
- Lamp is positioned away from operator line of sight for safety and
proper exposure to interior surfaces.
• PVC armrest
- Chemically treated, improves operator
comfort, easy to clean. 712 mm (28") standard size.
• Ergonomic lab chair
- Laboratory grade construction,
meets Class 100 cleanliness; alcohol
resistant PVC materials
- Adjustable 395-490 mm (15.6"-19.3")
• Ergonomic foot rest
- Angled, helps maintain proper
posture
- Adjustable height
- Anti-skid coating, chemical resistant finish
• IV bar, with hooks
- Stainless steel construction
- Available for all standard Esco cabinets
• Microscope viewing device
- Mounting and viewing pouch integrated into sash. Factory installed; specify when ordering.
Microbiological Testing
Esco performs testing in accordance
with more than 10 of the world’s most
recognized standards for local, regional
and international criteria.
Testing in our microbiology laboratory
is conducted according to NSF/ANSI 49:
2004, EN12469:2000, and JIS K3800:2005.
An NSF-accredited biohazard cabinet
field certifier is available in-house fulltime to supervise all testing work, using
harmless Bacillus atrophaeus (formerly
Bacillus Subtilis) bacteria that is used to
challenge the cabinet, then incubated
Bacterial Population (10 fold)
Antimicrobial Powder-Coating
All exterior painted surfaces are powder-coated
with Esco Isocide, an antimicrobial inhibitor to
minimize contamination. Isocide is integrated into
the coating substrate and cannot wash out or
diminish by repeated cleaning. Performance results
are available upon request. Contact Esco or your
Esco Sales Representative for details.
8
6
4
2
0
4 hours
8 hours
12 hours
16 hours
Contact Time in Hours
20 hours
24 hours
Cabinet Filtration System (Duo, Stainless Steel Side Walls)
Side capture zones
Dynamic air barrier, inflow and forward-directed downflow air converge
Ambient air is pulled through the
perforations located towards the work
zone front to prevent contamination of
the work surface and work product. The
inflow does not mix with the clean air
within the cabinet work zone. Inflow air
travels through a return path toward the
common air plenum (blower plenum) at
the top of the cabinet.
Dual blowers and dual exhaust filters
provide an added measure of protection.
If the primary blower fails, the secondary
blower still pushes the air across the
exhaust filters to maintain inflow and
containment.
ULPA-filtered air
Unfiltered / potentially contaminated air
Room air / Inflow air
Approximately 35% of the air in the
common plenum is exhausted through
the ULPA filter to the room. The
remaining 65% of the air is passed
through the downflow ULPA filter and
into the work area as a vertical laminar
flow air stream bathing the work surface
in clean air.
The uniform, non-turbulent air stream
protects against cross contamination
within and throughout the work area.
Near the work surface, the downflow
air stream splits with a portion moving
toward the front air grille, and the
remainder moving to the rear air grille.
A small portion of the ULPA filtered
downflow enters the intake perforations
at the side capture zones at a higher
velocity (small blue arrows).
A combination of inflow and downflow
air streams forms an air barrier that
prevents contaminated room air from
entering the work zone, and prevents
work surface emissions from escaping
the work zone.
Air returns to the common air plenum
where the 35% exhaust and 65%
recirculation process is continued.
Airstream Duo Model AC2 (D-Series) Biological Safety Cabinet Technical Specifications, Stainless Steel Side Walls, Dual Blowers
1
9
1400 mm (55.1")
3
11
10
4
5
670 mm (26.4")
1475 mm (58")
2
12
6
7
8
965 / 1270 / 1575 / 1880 mm
(40.7" / 52.7" / 64.7" / 76.7")
560 mm (22")
732 mm (28.8")
1035 / 1340 / 1645 / 1950 mm
(40.7" / 52.7" / 64.7" / 76.7")
1. Exhaust ULPA filter
2. Blower
3. Downflow ULPA filter
4. Standard UV light Retrofit Kit Provision
5. IV bar Retrofit Kit provision 6. Plugged service fixture provisions
(2 on each side)
7. Stainless steel work zone
8. Electrical outlet Retrofit Kit provision 10. Fluorescent lamp
(2 & 3 ft model - only 1 single outlet 11. Esco Sentinel microprocessor in work zone left side wall)
control system
9. Electrical panel
12. Tempered glass sash window
Biological Safety Cabinet • Class II Microbiological Safety Cabinets (E-Series, S-Series and D-Series)
General Specifications, Airstream Class II, Biological Safety Cabinets (E-Series)
Note to customer: Insert electrical voltage number into last model number digits _ when ordering
Model
AC2-2E_
Nominal Size
AC2-3E_
AC2-4E__
AC2-5E__
AC2-6E__
0.6 meters (2')
0.9 meters (3')
1.2 meters (4')
1.5 meters (5')
1.8 meters (6')
External Dimensions
(W x D x H)
730 x 740 x 1435 mm
28.7 " x 29.1" x 56.5"
1035 x 740 x 1360 mm
40.7" x 29.1" x 52.9"
1340 x 740 x 1360 mm
52.7" x 29.1" x 52.9"
1645 x 740 x 1360 mm
64.7" x 29.1" x 52.9"
1950 x 740 x 1360 mm
76.7" x 29.1" x 52.9"
Internal Work Area, Dimensions
(W x D x H)
660 x 560 x 670 mm
26.0" x 22.0" x 26.4"
965 x 560 x 670 mm
38.0" x 22.0" x 26.4"
1270 x 560 x 670 mm
50.0" x 22.0" x 26.4"
1575 x 560 x 670 mm
62.0" x 22.0" x 26.4"
1880 x 560 x 670 mm
74.0" x 22.0" x 26.4"
0.29 m2 (3.1 sq.ft)
0.43 m2 (4.6 sq.ft)
0.58 m2 (6.2 sq.ft)
0.73 m2 (7.7 sq.ft)
0.87 m2 (9.3 sq.ft)
Internal Work Area, Space
Average
Airflow
Velocity
Airflow
Volume
Inflow
0.45 m/s (90 fpm) at initial setpoint, audible/visual alarm will activate at 0.40 m/s (80 fpm)
Downflow
0.30 m/s (60 fpm) at initial setpoint with uniformity of better than +/- 20%
Inflow
185 m3 /h (109 cfm)
270 m3 /h (159 cfm)
356 m3 /h ( 209 cfm)
441 m3 /h (260 cfm)
527 m3 /h (310 cfm)
Downflow
423 m /h (249 cfm)
563 m /h (331 cfm)
741 m /h (436 cfm)
919 m /h (591 cfm)
1096 m3 /h (645 cfm)
Exhaust
185 m3 /h (109 cfm)
270 m3 /h (159 cfm)
356 m3 /h (209 cfm)
441 m3 /h (260 cfm)
527 m3 /h (310 cfm)
3
3
3
3
ULPA Filter
Typical
Efficiency
Downflow
Sound
Emission
(Typical)
NSF 49
<65 dBA
<64 dBA
<65 dBA
<65 dBA
<66 dBA
EN 12469
<62 dBA
<61 dBA
<62 dBA
<62 dBA
<63 dBA
>900 Lux
(> 84 foot candles)
>1130 Lux
(>105 foot candles)
>1280 Lux
(>119 foot candles)
>1050 Lux
(>97 foot candles)
>1220 Lux
(>113 foot candles)
Fluorescent Light Intensity At Zero
Ambient
Cabinet
Construction
>99.999% at 0.1 to 0.3 microns as per IEST-RP-CC001.3 USA
>99.995% at MPPS as per EN 1822 (H-14) EU
Exhaust
Main Body
1.2 mm (0.06") 16 gauge electrogalvanized steel with white oven-baked epoxy Isocide
antimicrobial powder coated finish
Work Zone
1.5 mm (0.06") 16 gauge stainless steel, type 304, with 4B finish
Side Walls
Electrical*
UV absorbing tempered glass, 5 mm (0.2"), colorless and transparent
220-240V, AC, 50Hz, 1ø
AC2-2E1
AC2-3E1
AC2-4E1
AC2-5E1
AC2-6E1
110-130V, AC, 60Hz, 1ø
AC2-2E2
AC2-3E2
AC2-4E2
AC2-5E2
AC2-6E2
220-240V, AC, 60Hz, 1ø
AC2-2E3
AC2-3E3
AC2-4E3
AC2-5E3
AC2-6E3
160 kg (353 lbs)
177 kg (390 lbs)
277 kg (611 lbs)
270 kg (595 lbs)
300 kg (661 lbs)
Net Weight**
Shipping Weight**
187 kg (412 lbs)
230 kg (507 lbs)
322 kg (710 lbs)
326 kg (718 lbs)
354 kg (780 lbs)
Shipping Dimensions,
Maximum (W x D x H)**
850 x 860 x 1750 mm
33.5" x 33.9" x 68.9"
1140 x 890 x 1715 mm
44.9" x 35" x 67.5"
1440 x 860 x 1650 mm
56.7" x 33.9" x 65"
1750 x 860 x 1650 mm
68.9" x 33.9" x 65"
2060 x 860 x 1680 mm
81.1" x 33.9" x 66.1"
Shipping Volume, Maximum**
1.28 m3 (45.6 cu.ft.)
1.6 m3 (57 cu.ft.)
2.04 m3 (72 cu.ft.)
2.48 m3 (88 cu.ft.)
2.98 m3 (105 cu.ft.)
* Additional voltages may be available; contact Esco for ordering information.
** Cabinet only; excludes optional stand
for 48 hours and the Colony Forming
Units (CFU) are counted to determine the
testing results. Increased microbiological
challenge tests with objects inside the
cabinet work zone, Bunsen burner,
external airflow disturbance, and HumanAs-Mannequin test adapted from Fume
Hood development were performed to
simulate real-world conditions.
Personnel Protection Test
The test objective is to evaluate the safety
of the cabinet for the personnel operating
on potentially hazardous samples in the
cabinet work zone.
• A nebulizer containing 55 mL of 5 to
8 x 108 spores/mL B.atrophaeus spores
is placed inside the work zone, 10 cm
(4") behind the front opening sash.
• Target slit air samplers and impingers
are placed outside the work zone to
capture possibly escaping B.atrophaeus
spores, then the sample is incubated.
• Acceptance: The number of Bacillus
atrophaeus CFU recovered from the
agar plates shall not exceed 10 CFU
per test.
Product Protection Test
The test objective is to determine cabinet
protection to the product/samples inside
the cabinet work zone from environmental
contaminants.
• A nebulizer containing 55 mL of 5 to 8
x 106 spores/mL B.atrophaeus is placed
at 10 cm (4") in front of sash window.
• Target agar plates are placed throughout the entire work surface.
• Acceptance: The number of Bacillus
atrophaeus CFU recovered from the
agar plates shall not exceed 5 CFU per
test.
Cross Contamination Test
The test objective is to evaluate cabinet
protection from cross contamination of
samples placed simultaneously inside the
work zone.
• A nebulizer containing 55 mL of spores
(5 to 8 x 104/mL) is placed against one
of the work zone sidewalls.
• Target agar plates are placed 360 mm
(14") away from the same side wall
General Specifications, Airstream Class II, Biological Safety Cabinets (S-Series)
Note to customer: Insert electrical voltage number into last model number digits _ when ordering
Model
AC2-3S_
Nominal Size
AC2-5S_
AC2-6S_
0.9 meters (3')
1.2 meters (4')
1.5 meters (5')
1.8 meters (6')
External Dimensions
(W x D x H)
1035 x 740 x 1360 mm
40.7" x 29.1" x 52.9"
1340 x 740 x 1360 mm
52.7" x 29.1" x 52.9"
1645 x 740 x 1360 mm
64.7" x 29.1" x 52.9"
1950 x 740 x 1360 mm
76.7" x 29.1" x 52.9"
Internal Work Area, Dimensions
(W x D x H)
965 x 560 x 670 mm
38.0" x 22.0" x 26.4"
1270 x 560 x 670 mm
50.0" x 22.0" x 26.4"
1575 x 560 x 670 mm
62.0" x 22.0" x 26.4"
1880 x 560 x 670 mm
74.0" x 22.0" x 26.4"
0.43 m2 (4.7 sq.ft)
0.58 m2 (6.2 sq.ft)
0.73 m2 (7.7 sq.ft)
0.87 m2 (9.3 sq.ft)
Internal Work Area, Space
Average Airflow
Velocity
Airflow Volume
Inflow
0.45 m/s (90 fpm) at initial setpoint, audible/visual alarm will activate at 0.40 m/s (80 fpm)
Downflow
0.30 m/s (60 fpm) at initial setpoint with uniformity of better than +/- 20%
Inflow
270 m3 /h (159 cfm)
356 m3 /h ( 209 cfm)
441 m3 /h (260 cfm)
527 m3 /h (310 cfm)
Downflow
563 m3 /h (331 cfm)
741 m3 /h (436 cfm)
919 m3 /h (591 cfm)
1096 m3 /h (645 cfm)
Exhaust
270 m /h (159 cfm)
356 m /h (209 cfm)
441 m /h (260 cfm)
527 m3 /h (310 cfm)
3
3
3
ULPA Filter
Typical
Efficiency
Downflow
Sound Emission
(Typical)
NSF / ANSI 49
<64 dBA
<65 dBA
<65 dBA
<66 dBA
EN 12469
<61 dBA
<62 dBA
<62 dBA
<63 dBA
>1040 Lux
(>97 foot candles)
>1190 Lux
(>111 foot candles)
>920 Lux
(>85 foot candles)
>1020 Lux
(>95 foot candles)
>99.999% at 0.1 to 0.3 microns as per IEST-RP-CC001.3 USA
>99.995% at MPPS as per EN 1822 (H-14) EU
Exhaust
Fluorescent Light Intensity At Zero
Ambient
Cabinet
Construction
10
AC2-4S_
Main Body
1.2 mm (0.06") 16 gauge electrogalvanized steel with white oven-baked epoxy Isocide
antimicrobial powder coated finish
Work Zone
1.5 mm (0.06") 16 gauge stainless steel, type 304, with 4B finish
Side Walls
1.2 mm (0.05") 18 gauge stainless steel, type 304
220-240V, AC, 50Hz, 1ø
AC2-3S1
AC2-4S1
AC2-5S1
AC2-6S1
110-130V, AC, 60Hz, 1ø
AC2-3S2
AC2-4S2
AC2-5S2
AC2-6S2
220-240V, AC, 60Hz, 1ø
AC2-3S3
AC2-4S3
AC2-5S3
AC2-6S3
Net Weight**
180 kg (397 lbs)
218 kg (481 lbs)
295 kg (650 lbs)
305 kg (672 lbs)
Shipping Weight**
230 kg (507 lbs)
292 kg (644 lbs)
426 kg (939 lbs)
354 kg (780 lbs)
1130 x 860 x 1650 mm
44.5" x 33.9" x 65.0"
1440 x 860 x 1650 mm
56.7" x 33.9" x 65"
1750 x 860 x 1650 mm
68.9" x 33.9" x 65"
2100 x 950 x 1880 mm
82.7" x 37.4" x 74.0"
1.6 m3 (57 cu.ft.)
2.04 m3 (72 cu.ft.)
2.48 m3 (88 cu.ft.)
3.75 m3 (132 cu.ft.)
Electrical*
Shipping Dimensions,
Maximum (W x D x H)**
Shipping Volume, Maximum**
* Additional voltages may be available; contact Esco for ordering information.
** Cabinet only; excludes optional stand.
• Acceptance: The number of Bacillus
atrophaeus CFU recovered on agar plates
shall not exceed 2 CFU per test.
KI Discus Containment Test
According to EN 12469:2000
(Potassium Iodide)
Esco is currently one of the few companies
in the world equipped to perform the KI
Discus test for our customers. The KI
Discus test is defined in the European
Standard for microbiological safety
cabinets, EN12469:2000, as a test method
for validating the operator/personnel
protection capabilities of the cabinet.
• The KI-Discus takes only 45 minutes as
opposed to 2 days for microbiological
testing.
• The KI Discus test shows excellent
correlation with the microbiological
test method for operator protection,
• Thus, each Esco Airstream FC2 model
is type tested using the KI Discus
method for operator safety.
For Microbiological
Safety Cabinets*
Standards
Compliance
Type-tested to
EN 12469:2000, Europe
For Air Quality
ISO 14644.1 Class 3, Worldwide
IEST-G-CC1001, USA
IEST-G-CC1002, USA
and is useful for validating the actual
containment performance of the
cabinet on-site.
For Filtration
For Electrical Safety
IEST-RP-CC034.1, Worldwide
IEC 61010-1, Worldwide
IEST-RP-CC007.1, Worldwide
IEST-RP-CC001.3, Worldwide
EN 1822, Europe
* For details on most recent certifications contact Esco or your Esco Sales Representative.
Biological Safety Cabinet • Class II Microbiological Safety Cabinets (E-Series, S-Series and D-Series)
EN 61010-1, Europe
UL 61010A-1, USA
CAN/ CSA C22.2 No. 61010-1,
Canada
General Specifications, Airstream Class II, Biological Safety Cabinets (D-Series)
Note to customer: Insert electrical voltage number into last model number digits _ when ordering
Model
AC2-4D_
Nominal Size
AC2-6D_
1.2 meters (4')
1.8 meters (6')
External Dimensions
(W x D x H)
1340 x 740 x 1475 mm
52.8" x 29.1" x 58"
1950 x 740 x 1360 mm
76.7" x 29.1" x 52.9"
Internal Work Area, Dimensions
(W x D x H)
1270 x 560 x 670 mm
50" x 22.0" x 26.4"
1880 x 560 x 670 mm
74.0" x 22.0" x 26.4"
0.58 m2 (sq.ft)
0.87 m2 (9.3 sq.ft)
Internal Work Area, Space
Average Airflow
Velocity
Airflow Volume
Inflow
0.45 m/s (90 fpm) at initial setpoint, audible/visual alarm will activate at 0.40 m/s (80 fpm)
Downflow
0.30 m/s (60 fpm) at initial setpoint with uniformity of better than +/- 20%
Inflow
270 m3 /h (159 cfm)
527 m3 /h (310 cfm)
Downflow
563 m3 /h (331 cfm)
1096 m3 /h (645 cfm)
Exhaust
270 m /h (159 cfm)
527 m3 /h (310 cfm)
3
ULPA Filter
Typical
Efficiency
Downflow
Sound Emission
(Typical)
NSF / ANSI 49
<65.5 dBA
<66 dBA
EN 12469
<62.5 dBA
<63 dBA
>1200 Lux (>111.5 foot candles)
>1020 Lux (>95 foot candles)
Fluorescent Light Intensity At Zero
Ambient
Cabinet
Construction
>99.999% at 0.1 to 0.3 microns as per IEST-RP-CC001.3 USA
>99.995% at MPPS as per EN 1822 (H-14) EU
Exhaust
Main Body
1.5 mm (0.06") 16 gauge electrogalvanized steel with white oven-baked epoxy Isocide
antimicrobial powder coated finish
Work Zone
1.2 mm (0.06") 16 gauge stainless steel, type 304, with 4B finish
Side Walls
1.2 mm (0.05") 18 gauge stainless steel, type 304
220-240V, AC, 50Hz, 1ø
AC2-4D1
AC2-6D1
110-130V, AC, 60Hz, 1ø
AC2-3D2
AC2-6D2
220-240V, AC, 60Hz, 1ø
AC2-3D3
AC2-6D3
Net Weight**
223.5 kg (492.7 lbs)
305 kg (672 lbs)
Shipping Weight**
245.5 kg (541.2 lbs)
354 kg (780 lbs)
1500 x 950 x 1880 mm
59" x 37.4" x 74"
2100 x 950 x 1880 mm
82.7" x 37.4" x 74"
2.68 m3 (96.4 cu.ft.)
3.75 m3 (132 cu.ft.)
Electrical*
Shipping Dimensions,
Maximum (W x D x H)**
Shipping Volume, Maximum**
* Additional voltages may be available; contact Esco for ordering information.
** Cabinet only; excludes optional stand
Comprehensive Performance Testing At Esco
Every Airstream model manufactured by Esco is
individually tested, documented by serial number and
validated with the following test methods.
Inflow / downflow velocity
PAO aerosol challenge for filter integrity
Light, noise and vibration
Airflow pattern visualization
Electrical safety to IEC61010-1
Additional microbiological testing is performed
on statistical sampling basis.
11
ESCO Airstream® E-Series Class II Biosafety Cabinet - 2 feet version
ze For All
Compact Si
es!
Laboratori
4” / 73cm
2’
:
th
id
W
l
Tota
th: 2’ / 66cm
Internal Wid
Independently type-tested by Health Protection Agency
(Porton Down, UK) to the EN 12469, European Standard for
Class II biosafety cabinets, Esco Airstream® Class II biosafety
cabinets are now available in compact 2ft widths, designed for
laboratories with space constraints.
This model features many key innovations for which Esco
is recognized for: mini-pleat separatorless ULPA filter
technology, the best product protection in the world,
microprocessor-based control system, external rotor motors,
superior filter mechanical construction.
Esco Airstream® Class II biosafety cabinets are equipped
with ULPA filters (typical efficiency of 99.9998% at 0.3
microns) that offer a significantly higher efficiency than
conventional HEPA filters (99.99% at 0.3 microns) used by most
other manufacturers.
MAIN FILTRATION AGENTS
 ISO Class 3 air cleanliness within
workzone as per ISO 14644.1 (equivalent
to Class 1 as per the US Federal Standard
209E, 100 times cleaner than the usual
Class 100 classification on cabinets offered
by the competition).
 Minipleat separatorless ULPA filter
technology, operating at the typical
efficiency of 99.9999% at MPPS, 0.3 and
0.12 microns provides better operator,
product and environmental protection than
conventional HEPA filters. This cabinet
employs 2 ULPA filters, 1 for downflow and
1 for exhaust airflow filtration.
CABINET CONTROL SYSTEM
 Esco Sentinel™ Microprocessor
Cabinet Control System allows the user
to easily access all cabinet functions. The
control system monitors and displays
cabinet airflow constantly on the control
LCD screen, prompting the user with
audible and visual alarms in case of any
unsafe condition.
 Fail-safe system ensures that in case of
airflow failure, the cabinet automatically
shuts down to ensure safety to the user
and the environment.
 True airflow velocity (both for downflow
and inflow velocity) sensing technology,
with temperature compensation for improved sensor accuracy.
 Audible and visual alarms for low airflow,
unsafe sash positions.
 Automatic warm-up cycle is enabled
upon turning on the cabinet, ensuring all
contaminants are purged from the cabinet
workzone before the operator can use the
cabinet. All menu functions are inactive
during this period. Automatic post-purge
cycle is also enabled at shutdown, ensuring
all residue contaminants are purged out of
the workzone before deactivation.
 An admin PIN can be set to restrict
unauthorized access to all menu functions.
ERGONOMIC FEATURES
 Ergonomic sloped front design allows
for maximum visibility into the workzone.
operating costs; extremely low noise and
vibration levels (less than 60dBA at working
position) due to proprietary construction
and mounting technology.
 The cabinet’s airflow is selfregulating: the motor / blower system
is able to compensate automatically to
maintain airflow as the filter is loaded with
particulates.
 The frameless tempered glass
sliding front sash and tempered glass
sidewalls provide a greater visibility into
the workzone.
 Built-in solid state variable speed
controller(s) (infinitely adjustable from zero
to the maximum setting) with built-in RFI
and noise filters is superior to conventional
“step” controllers.
 Built-in 5000k fluorescent lighting
offers excellent illumination throughout
the workzone. Electronically-ballasted
lighting system is instant-start, nonflickering and energy-efficient.
 Superior electrical safety for the
operator; all electrical components are
UL listed or UL recognized; all cabinets are
factory tested for electrical safety after
production at manufacturing site.
 Cabinet armrest is raised above the
workzone to ensure that the operator’s
arms do not block the front inflow
perforations. (Optional PVC soft armrestpadding available upon request. Refer to
our Accessories catalogue.)*
 Designed to meet the general safety
requirements of the IEC 61010-1 / EN
61010-1 / UL 61010A-1 / CSA C22.2 No.
1010.1-92.
CABINET BODY CONSTRUCTION
 All components designed for maximum
chemical resistance for long service life
and durability. All cabinet components are
cleanroom compatible.
 Industrial-grade main body constructed
of electrogalvanised steel: with an abrasionresistant white oven-baked powder-coated
finish. Attractive two-piece stainless steel
work trays are easy to remove for purposes
of cleaning and auto-claving.
 Permanently lubricated direct drive
centrifugal blower(s); energy efficient
external rotor motor type design reduces
OPTIONAL ACCESSORIES
Support Stand with
castor wheels or
levelling feet.Cabinet
construction allows for
the cabinet to be lifted
directly on to our optional
support stand (available
with castor wheels or
levelling feet).
Exhaust collar for nonairtight thimble ducting
of the cabinet.
For other optional and
ergonomic accessories,
check our catalogue:
http://biotech.escoglobal.com/PDF/optErgAcc.pdf
Esco Catalogue Revision No: AC2.2ft.v1.12.2005
Specifications subject to change without notice.
©2005 Esco Micro Pte. Ltd.
173 mm / 6.8”
Technical Specifications and Engineering Diagram
15
1
2
1435 mm / 56.5”
7
3
12
8
4
5
13
9
14
6
670 mm / 26.4”
Work zone heignt
10
11
660 mm / 26.0”
732 mm / 28.8”
730 mm / 28.7”
1. Exhaust ULPA Filter 2. Blower 3. Downflow ULPA Filter 4. UV Light Retrofit Kit™ Provision 5. IV Bar Retrofit Kit™ Provision
6. Service Fixture Retrofit Kit™ Provisions (2 One Each Side) 7. Electrical & Electronics Panel 8. Fluorescent Lamps
9. Tempered Glass Sidewalls 10. Stainless Steel Divided Work Tray (2pcs) 11. Stainless Steel Armrest
12. Esco Sentinel™ Microprocessor Control System 13. Tempered Glass Sliding Sash
14. Electrical Outlet Retrofit Kit™ Provision 15. Optional Exhaust Collar
General Specifications
AC2-2EX
External Dimensions (L x W x H)
730 x 732 x 1435 mm / 28.7” x 28.8” x 56.5”
Internal Work Zone (L x W x H)
660 x 560 x 670 mm / 26.0” x 22.0” x 26.4”
Standards Compliance
EN 12469:2000 type-tested (exceeds requirements for Class II microbiological safety cabinets)
Air cleanliness: ISO 14644.1 Class 3, IEST-G-CC1001, IEST-G-CC1002 and other equivalent requirements
Filter performance: IEST-RP-CC034.1, IEST-RP-CC007.1, IEST-RP-CC001.3 and EN1822
Electrical safety: IEC 61010-1 / EN 61010-1 / UL 3101-1 / CSA C22.2 No. 1010.1-92
Inflow
Average Airflow
Velocities
Downflow
Airflow Volumes
At Initial Airflow
Velocity Setpoints
Initial setpoint: 0.45 m/s or 90 fpm (audible / visual alarm will activate at 0.4m/s or 80fpm)
Initial setpoint: 0.31 m/s or 61 fpm (uniformity is +/-20%)
Inflow
159 cmh / 94 cfm
Downflow (70%)
384 cmh / 226 cfm
Exhaust (30%)
Cleanliness Within Working Area
Downflow and Exhaust Filter Type
Filter Efficiency Ratings
Noise Level
Light Intensity
ULPA filter with integral metal guards and filter frame gaskets; fully compliant with EN 1822 and IEST-RPCC001.3 requirements (each cabinet has individual downflow and exhaust filters)
Minimum: 99.9995% at 0.3µm / 99.9994% at 0.12µm / 99.9991% at MPPS
Typical: 99.9999% at 0.3µm / 99.9999% at 0.12µm / 99.9998% at MPPS
Typically <60 dBA at initial blower speed setting, according to EN 12469:2000
(subject to acoustic properties of test environment)
>750 Lux / >70 foot candles, (measured at work surface level (zero background) as per NSF49 test grid)
Main Body Construction
1.5mmt / 0.06” / 16 gauge electro-galvanized steel with white oven-baked epoxy powder-coated finish
Side Wall Construction
Colourless and transparent UV-absorbing 5mm / 0.2” tempered glass
Work Surface Construction
Maximum
Power
Consumption
/ Current
159 cmh / 94 cfm
ISO 14644.1 Class 3, US Federal Standard 209E Class 1 / M1.5, AS 1386 Class 1.5, JIS B9920 Class
3, BS5295 Class C, Class M10,000 as per KS 27030.1 and equivalent classes of VDI 2083 and AFNOR
X44101
1.2 mmt / 0.05” / 18 gauge stainless steel grade 304 with BA finish
220-240VAC /
50Hz 1Ph
Cabinet Power / Current
Outlet Power / Current
1000VA / 5A
110-130VAC /
60Hz 1Ph
Cabinet Power / Current
385W / 3.5A
Outlet Power / Current
Net Weight (Approximate)
300W / 1.5A
500VA / 5A
160 kgs / 352 lbs
Max Shipping Weight
240 kgs / 530 lbs
Max Shipping Dimensions (L x W x H)
885 x 917 x 1690 mm / 34.8” x 36.1” x 66.5”
Max Shipping Volume
1.37 cbm / 48.4 cbf
ESCO
®
Esco Biotechnology Equipment Division
Your local distributor:
Esco Biotech is a highly focused manufacturer of laminar flow, biohazard
safety and other HEPA-filtered cabinets for the laboratory with a history
of quality cabinets since 1978. We are predominantly oriented towards the
international marketplace, with sales in more than 70 countries and 90% of
turnover exported. Our products have been independently tested to standards such as AS1807.5 and EN12469. Products are manufactured under
an ISO 9001 registered quality system.
21 Changi South Street 1 · Singapore 486 777 · Tel: +65 6542 0833 · Fax: +65 6542 6920 · Email: [email protected] · biotech.escoglobal.com
NSF 49, UL 61010, JIS K3800, SFDA YY-0569, EN 12469, SANS 12469
Labculture Class II, Type A2
Biosafety Cabinet, Model LA2-4A_.
Class II, Type A2 Biological Safety Cabinets
The Safety Solution for Life Science Laboratories
Main Features
Unique Esco Dynamic Chamber™ plenum.
Negative pressure plenum surrounds contaminated
positive pressure plenum; no fabric bags are used.
Dual, long-life ULPA filters for supply and exhaust
(per IEST-RP-CC001.3) with 99.999% efficiency for
particle size between 0.1 to 0.3 microns.
Angled supply filter matches cabinet profile to
achieve best downflow uniformity.
Esco Sentinel™ microprocessor.
Frameless, shatterproof sash is easier to clean.
Ergonomically angled front improves reach and comfort.
Actual work access height is 45 mm (1.8") higher
than tested sash opening.
Removable one-piece work surface simplifies cleaning.
Raised airflow grille maintains safety by preventing
blockage.
Optional UV lamp is located behind control
panel away from line of sight. (Does not
apply to 1.8 m / 6' models.)
Esco
antimicrobial coating on all
painted surfaces minimizes contamination.
Enhanced side-capture zones optimize
containment.
Work area on Esco 0.9 meter (3') cabinet is
equivalent to work area offered on larger 1.2 meter (4') conventional cabinet.
Each cabinet is KI-Discus tested for performance integrity.
Available in 0.9, 1.2, 1.5 and 1.8 meter models (3', 4', 5' and 6').
Shown with optional telescoping stand.
NSF 49, UL 61010, JIS K3800, SFDA YY-0569, EN 12469, SANS 12469
Esco Labculture has passed more performance
tests in more languages, for more certifications
throughout more countries than any other
biological safety cabinet in the world.
Biological Safety Cabinet • Class II, Type A2 Biological Safety Cabinets
• The ULPA downflow (supply) filter is
tilted proportional to the cabinet front
angle to direct more air forward to the
front air grille.
• The inflow velocity, downflow velocity,
air flow path, and intake geometry are
precision tuned and tested to create an
optimum air curtain on the front aperture;
this curtain maintains personnel and
product protection even in the unlikely
event of a severe inflow or downflow
imbalance that would compromise
protection in a conventional cabinet.
Integrated Filtration System
A combination of a supply ULPA filter and
an exhaust ULPA filter give the Labculture
cabinet a fully integrated performance
envelope for product, operator and
environmental protection.
Operator, Product and Environmental Protection
The Esco Labculture Class II, Type A2 Biosafety Cabinet
provides operator, product and environmental protection
against Biosafety Levels 1, 2 and 3. This cabinet can be
used for handling Biohazard Level 4, provided that the
operator wears positive pressure suit.
Containment and Protection
The airflow ratio of 63% recirculation to
37% exhaust increases operator protection
beyond the 70%/30% ratio of conventional
biological safety cabinets.
• ULPA filters (per IEST-RP-CC001.3),
are tested to a typical efficiency of
>99.999% for 0.1 to 0.3 micron particles;
these provide better filtration capability
than conventional HEPA filters that
have a typical efficiency of >99.99% for
0.3 micron particles.
• The front grille has proportionally larger
perforations on the extreme left and
right side. Combined with the side air
gap between the work tray and the side
wall, the stronger side capture zones
increase protection in this critical area
where contaminants tend to bleed out
in conventional cabinets.
• The supply ULPA filter provides ISO Class
3 (per ISO14644.1) clean air to the work
surface in a gentle vertical laminar flow
for product protection.
• The exhaust ULPA filter traps biohazard
particles acquired from the work surface
before air is exhausted to the room,
offering personnel and environmental
protection.
• The exhaust filter media is protected
from mechanical damage by an
integrated metal screen guard, which is
absent from conventional HEPA filters.
• The top cabinet surface is slanted to
discourage users from placing objects
which could block the exhaust filter and
reduce inflow velocity.
• Integrated sash proximity contacts
sense proper sash position, serve as an
interlock for the UV lamp, and activate
an alarm if the sash is improperly
positioned.
• The magnetic switch eliminates the
chance of wear and tear typical of a
mechanical switch.
Esco cabinets use Swedish Camfil Farr® mini-pleat
filters without aluminum separators to increase filter
efficiency, minimize the chance of leakage, and to
prolong filter life. Filters include a lightweight
aluminum frame for structural stability and
elimination of swelling common to conventional
wood frames.
• The back of the sash can be easily
cleaned by removing the sash track
cover and swinging up the sash glass.
• The sash is counterbalanced for smooth,
lightweight operation.
Esco ULPA Filter Efficiency
• The convex inflow grille maintains
operator protection by discouraging
users from placing objects on it.
• Auto-purge holes located at the front
side walls eliminate eddy currents and
dead air pockets in the critical area
behind the sash window. Per NSF/ANSI
49 requirement, these side perforations
do not extend completely to the work
surface to prevent accidental liquid
spills in the work zone from entering
the side air column.
• The filter frame and media is constructed
in accordance with EN1822 requirements
for fire retardant properties.
Front Sash Assembly
Mini-pleat Separatorless Filter (left) vs.
Conventional Aluminium Separator Filter (right)
• The inflow and downflow balance is
precisely established by an external
exhaust damper and is adjustable
without decontaminating the cabinet.
• Inflow of room air enters the front air
grille to establish operator protection;
room air does not enter the work zone,
preventing product contamination.
• Modern separator-less mini-pleat filter
construction maximizes the filter surface
area to extend filter life and eliminate
possible filter media damage by thin
and sharp aluminum separators used in
conventional HEPA filter construction.
Esco cabinets use ULPA filters
(per IEST-RP-CC001.3) instead
of conventional HEPA filters
commonly found in biological
safety cabinets. While HEPA filters
offer 99.99% typical efficiency
at 0.3 micron level, ULPA filters
provide 99.999% typical efficiency
for particle sizes of 0.1 to 0.3
micron level.
Typical Penetration
Cabinet Filtration System
Side capture zones
Dynamic air barrier, inflow and forward-directed downflow air converge
Ambient air is pulled through the
perforations located towards the work
zone front to prevent contamination of
the work surface and work product. The
inflow does not mix with the clean air
within the cabinet work zone. Inflow air
travels through a return path toward the
common air plenum (blower plenum) at
the top of the cabinet.
ULPA-filtered air
Unfiltered / potentially contaminated air
Room air / Inflow air
Approximately 35% of the air in the
common plenum is exhausted through
the ULPA filter to the room. The
remaining 65% of the air is passed
through the downflow ULPA filter and
into the work area as a vertical laminar
flow air stream bathing the work surface
in clean air.
• The blower/motor can maintain airflow
for a 150% increase in pressure loss
over clean filters without manual
speed control adjustment, or 210%
increase in pressure drop across the filter
with manual speed control adjustment.
(Applies to 1.2 meter / 4 ft. model; contact
Esco for information on other models.)
• The safety glass maintains containment
if the sash is accidentally broken during
cabinet operation.
• The permanently lubricated direct-drive
external rotor motor/blower reduces
operating costs.
The Labculture blower system is designed
for high performance operation,
maximum energy efficiency and minimal
maintenance.
• The external rotor motor design allows
for optimum cooling of the motor
during extended operations and extends
the motor bearing life.
A combination of inflow and downflow
air streams forms an air barrier that
prevents contaminated room air from
entering the work zone, and prevents
work surface emissions from escaping
the work zone.
Air returns to the common air plenum
where the 35% exhaust and 65%
recirculation process is continued.
The uniform, non-turbulent air stream
protects against cross contamination
within and throughout the work area.
• The counterbalance locking mechanism
is inherently safe; it locks the counterbalance in place if either of the 2 cables
is detached. The sash cable and cable
clip have rated strength of more than
6 times the weight of the sash window
of the largest Labculture Class II cabinet
(1.8 meter / 6 ft. model).
Blower Efficiency
Near the work surface, the downflow
air stream splits with a portion moving
toward the front air grille, and the
remainder moving to the rear air grille.
A small portion of the ULPA filtered
downflow enters the intake perforations
at the side capture zones at a higher
velocity (small blue arrows).
• Built-in RFI and electrical noise filters
eliminate interference with adjacent
instrumentation.
• An integral blower hour meter tracks
operating life for predictive maintenance
planning.
• To prevent fan damage, a paper-catch
grille traps papers or towels that may
drop down on the drain pan, preventing
them from being pulled into the column
by fan suction.
Esco Centrifugal Fan with External Rotor Motor (left) vs.
Conventional Fan with Standard Motor (right)
Esco cabinets use German made ebm-papst®
permanently lubricated, centrifugalmotor/
blowers with external rotor designs.
Integrated blades narrow the profile and
eliminate need for a motor shaft.
Motors are selected for energy efficiency,
compact design, and flat profile. The completely
integrated assembly optimizes motor cooling.
All rotating parts are unitized and balanced for smooth, quiet, vibration-free operation.
Biological Safety Cabinet • Class II, Type A2 Biological Safety Cabinets
Sentinel Microprocessor Control,
Alarm, Monitoring System
The Esco Sentinel™ microprocessor-based
control system supervises operation of all
cabinet functions.
• The control panel is located on the
center of the cabinet, and angled down
for easy access by the operator
• Continuous monitoring of cabinet
airflow is displayed on a bright, easy-toread LCD panel.
• An integrated, temperature-compensated, true airflow velocity sensor
provides an accurate airflow reading
despite room temperature fluctuation.
• All electronic parts are contained inside
a plug-and-play module that permits
easy exchange if required.
• Microprocessor software updates are
available from Esco for download via
the Internet.
Sentinel functions are factory set to default
to ON or OFF, depending on worldwide
destination and local preferences. Default
settings can be user activated through the
touchpad data entry access.
• Automatic start-up sequence will
prepare the cabinet for normal operation
and advise when safe conditions are
established.
• An administrator controlled PIN
(Personal Identification Number) can be
set to restrict access to main menu.
Touchpad data entry buttons permit
control settings and access to diagnostics,
default settings and hierarchical menus.
Color coded indicator lamps display green
for primary function (fan operation); blue
for secondary function (fluorescent lights
and electrical outlet); and orange for
caution (UV lamp ON).
Sentinel Microprocessor Control System, Programmable
Programmable automatic UV light
timer simplifies operation, enhances
contamination control, extends UV
lamp life and saves energy.
A graphical interface indicates cabinet performance.
Digital read-out with alpha-numeric display indicates
all input, status and alarm functions.
All functions can be user activated through touch-pad
programming access; see Operations Manual.
When programmed ON • the start-up sequence confirms status with Air Safe and local time display.
• the Personal Identification Number (PIN) access restricts unauthorized adjustments.
• an airflow alarm warns of deviations from normal velocities.
• The airflow alarm can be activated
or deactivated depending on user
preference and nature of the work.
• The interior work area is formed from a
single piece of stainless-steel with large
radius corners to simplify cleaning.
Consult your Esco Operating Manual
or contact your Sales Representative
for information on user-preference
programming capabilities built into the
Sentinel microprocessor platform.
• The cabinet work zone has no welded
joints to collect contaminants or rust.
• There are no screws in on the front or
sides to trap contaminants or complicate
cleaning.
• All stainless steel work surfaces are
accessible for cleaning.
• Optional service fittings are offset for
easier access.
• Tray components lift and remove to
provide easy access and encourage
surface decontamination.
• External plumbing is concealed behind
trim panels to maintain aesthetics.
Cabinet Construction
Robust construction and enhanced safety
features qualify the cabinet for the most
demanding laboratory applications. The
cabinet is fully assembled and ready to install
and operate when shipped.
• A recessed central area and stainless
steel drain pan channels spills and
prevent liquids from entering the lower
filtration and blower systems.
• The drain pan is flush with the side
walls to eliminate concealed or hardto-clean spaces.
• External surfaces are coated with Esco
Isocide™ antimicrobial coating to protect
against surface contamination and
inhibit bacterial growth. Isocide
eliminates 99.9% of surface bacteria
within 24 hours of exposure.
Robust Cabinet Construction and Enhanced Safety Features
Service fixtures are offset for easier reach. Standard cabinets include two fixture provisions on each sidewall (one provision on each sidewall for 0.9 meter/3 ft. cabinet). Electrical outlets are mounted below service fixtures to minimize obstructions.
Helpful for certifiers, the hinged maintenance assembly opens to a fixed position on integrated, gas spring struts providing front service access.
All key components, with the exception of the blower/motor assembly, are mounted outside the air stream and away from contaminated air to permit service without decontamination. These include fluorescent lamps, UV lamp, electrical harnesses, electronic boards and microprocessor control.
Panels enclosing potentially hazardous areas or components such as microbiological contamination or electrical shock are color-coded red to warn service technician.
The telescoping Dynamic Chamber™ plenum minimizes physical lifting and accelerates filter change when required.
Work area containment is maintained even when removable components are lifted out for cleaning.
The one piece stainless steel work tray edges are radiused and easy to clean without crevices or joints.
The lower drain trough is a single-piece fabrication with wide open angles and a channel to direct spills to the drain.
The closed sidewall contains no perforations, air return slots or other hidden areas where contaminants can accumulate.
Standards
Compliance
Biosafety Cabinets
Air Quality
Filtration
Electrical Safety
NSF / ANSI 49, USA*
EN 12469, Europe
JIS K 3800, Japan
SFDA YY-0569, China
ISO 14644.1, Class 3, Worldwide
JIS B9920, Class 3, Japan
JIS BS5295, Class 3, Japan
US Fed Std 209E, Class 1 USA
EN-1822 (H14), Europe
IEST-RP-CC001.3, USA
IEST-RP-CC007, USA
IEST-RP-CC034.1, USA
UL-C-61010A-1, USA
CSA22.2, No.1010-192, Canada
EN-61010-1, Europe
IEC61010-1, Worldwide
* Please refer to the specifications table on page 8 for the model listing.
• The front armrest is raised above the
workzone to improve comfort and to
ensure that the operator’s arms do not
block the forward airflow perforations.
Dynamic Chamber™ Plenum Design
• The optional adjustable support stand
provides work surface height control.
• The frameless sash eliminates operator's
line of sight blockage
• A generous sash opening allows for
easier access into the work zone,
provides ample room for transferring of
small equipment.
Negative pressure
Positive pressure
The Esco triple-wall design creates a Dynamic Chamber
plenum which surrounds contaminated areas with
negative pressure, preventing the possibility of
contamination from leaks in filter seal, gasket or
cabinet structure. The third wall conceals utilities.
Comfortable Ergonomic Design
• The 10° angled viewing window and
narrow profile front grille improves
reach into the work area.
• The instant-start 5000k fluorescent
lamp operates on an electronic ballast
to reduce heat, improve comfort and
conserve energy.
• The lamp delivers uniform lighting to
the work surface for greater comfort,
reduced glare and improved productivity;
see Technical Specifications.
Electrical Safety and Certification
All components meet or exceed applicable
safety requirements.
• Each cabinet is individually tested for
electrical safety at factory.
• Documentation specific to each cabinet
serial number is maintained on file.
• UL Listed for USA and Canada.
• Certified to all major world standards
for biological safety cabinets, including
the NSF / ANSI 49 and EN 12469 (refer
to Standards Compliance table on this
page for the full list).
• Contact Esco or your Sales Representative
for site preparation information; see
Electrical Specifications.
The Labculture cabinet is warranted for
3 years excluding consumable parts and
accessories.
• Each cabinet is shipped with a comprehensive user’s manual complete with a
report documenting all test procedures.
• Additional IQ/OQ/PQ documentation
is available upon request.
• Contact your local Sales Representative
for specific warranty details or documentation requests.
Accessories and Options
Esco offers a variety of options and
accessories to meet local applications.
Contact Esco or your local Sales Representative for ordering information.
Support Stands
• Fixed height, available 711 mm (28")
or 864 mm (34"), ±38.1 mm (1.5")
-With leveling feet
-With casters
•
Adjustable height, hydraulic range
711 mm to 864 mm (28" to 34")
-With leveling feet
-With casters
• Telescoping height stand for leveling feet, nominal range 660 mm or 960 mm (26" or 37.8")
•
Telescoping height stand for casters, nominal range 660 mm or 880 mm (26" or 34.6")
-Adjustable in 25.4 mm (1") increments
Antimicrobial Powder-Coating
Bacterial Population (10 folds)
The LA2 cabinet is engineered for comfort,
utility value and safety.
• The sliding window can be fully
opened to insert and remove larger
instrumentation and equipment.
Warranty
All exterior painted surfaces are powder-coated
with Esco Isocide, an antimicrobial inhibitor to
minimize contamination. Isocide is integrated into
the coating substrate and cannot wash out or
diminish by repeated cleaning. Performance results
are available upon request. Contact Esco or your
Esco Sales Representative for details.
8
6
4
2
0
4 hours
8 hours
12 hours
16 hours
Contact Time in Hours
Biological Safety Cabinet • Class II, Type A2 Biological Safety Cabinets
20 hours
24 hours
16
955 mm / 1260 mm / 1565 mm / 1870 mm
(37.6"/ 49.6"/ 61.6"/ 73.6")
3
462 mm (1
8.
MAX. OPE 2")
NING
4a9
4b9
5
9
109
670 mm (26.4")
87
27
(Internal height for 110-130V, 60Hz
models of 1.2 m and1.8 m/4'
and 6' is 715 mm/28.1")
15
76
11
12
180 mm
(7.1")
1115 mm / 1420 mm / 1725 mm / 2030 mm
(43.9"/ 55.9"/ 67.9"/ 79.9")
139 mm
(5.5")
6
45 mm
(1.8")
14
16 1
ACC S
OPENES
ING
1540 mm (60.6")
13
(Exterior height for 110-130 V, 60 Hz models of 1.2 m and 1.8 m / 4' and 6' is 1640 mm / 64.6")
Model LA2 Biological Safety Cabinet Technical Specifications
815 mm (32.1")
0.9 meter (3')
1.2 meter (4')
1.5 meter (5')
1.8 meter (6')
Testing Opening Height
228.6 mm (9")
228.6 mm (9")
228.6 mm (9")
203.2 mm (8")
Working Access Height
274 mm (10.8")
274 mm (10.8")
274 mm (10.8")
218 mm (8.6")
*Cabinet Size
6. Electrical outlet Retrofit Kit provision (0.9 meter /3' model - one single outlet in workzone) (1.2, 1.5 and Downflow ULPA filter, angled
1.8 meter / 4',5' & 6' models - two UV light Retrofit Kit provision for 0.9, single outlets in workzone)
1.2 and 1.5 meter (3',4' & 5') models.
7. Electrical / Electronics panel
UV light Retrofit Kit provision for 1.8 8. Fluorescent lamp
meter (6') models.
9. Plugged service fixture provisions
IV-Bar Retrofit Kit provision
(2 on each side)
1. Exhaust ULPA filter
10. Stainless steel single-piece work tray
14. Safety glass sliding sash window
2. Blower
11. Stainless steel armrest
3.
12. Drain valve Retrofit Kit provision
15. Single-piece stainless steel back wall and side walls
4a.
4b.
5.
3. Esco Sentinel microprocessor control 16. Removable side panel for plumbing 1
access
system
Optional Exhaust Collar Positions for Thimble-Ducting for LA2 Models
610 mm
(24")
269 mm
(10.6")
129 mm
5.1"
249 mm
(ø9.8")
269 mm
(10.6")
333 mm
(13.1")
269 mm
(10.6")
Top View - LA2-3A_
Top View - LA2-5A_
691 mm
(27.2")
269 mm
(10.6")
269 mm
(10.6")
350 mm
(13.8")
Side View - All Sizes
Top View - LA2-4A_
Top View - LA2-6A_
General Specifications
North America Models
LA2-3A2
LA2-4A2
LA2-5A2
LA2-6A2
0.9 meters ( 3')
1.2 meters ( 4')
1.5 meters ( 5')
1.8 meters ( 6')
External Dimensions
(W x D x H)
1115 x 810 x 1540 mm
43.9" x 31.9" x 60.6"
1420 x 815 x 1640 mm
55.9" x 32.1" x 64.6"
1725 x 815 x 1540 mm
67.9" x 32.1" x 60.6"
2030 x 815 x 1640 mm
79.9" x 32.1" x 64.6"
Gross Internal Dimensions
(W x D x H)
955 x 623 x 670 mm
37.6" x 24.5" x 26.4"
1260 x 623 x 715 mm
49.6" x 24.5" x 28.1"
1565 x 623 x 670 mm
61.6" x 24.5" x 26.4"
1870 x 623 x 715 mm
73.6" x 24.5" x 28.1"
0.45 m2 (4.8 sq.ft.)
0.6 m2 (6.5 sq.ft.)
0.75 m2 (8.1 sq.ft.)
0.9 m2 (9.7 sq.ft.)
228.6 mm (9")
228.6 mm (9")
228.6 mm (9")
203.2 mm (8")
274 mm (10.8")
274 mm (10.8")
274 mm (10.8")
248 mm (9.8")
(USA, Canada, Mexico / 115 V, 60 Hz)
Nominal Size
Usable Work Area
Tested Opening
Working Opening
Average Airflow
Velocity
Airflow Volume
Inflow
.53 m/s (105 fpm)
Downflow
0.35 m/s (70 fpm)
0.35 m/s (70 fpm)
0.35 m/s (70 fpm)
0.33 m/s (65 fpm)
Inflow
419 m / h (246 cfm)
552 m / h (325 cfm)
685 m / h (403 cfm)
742 m3 / h (437 cfm)
Downflow, 63%
702 m3 / h (413 cfm)
924 m3 / h (544 cfm)
1147 m3 / h (675 cfm)
1289 m3 / h (758 cfm)
Exhaust, 37%
419 m / h (246 cfm)
552 m / h (325 cfm)
685 m / h (403 cfm)
742 m3 / h (437 cfm)
Required Exhaust
With Optional
Thimble Exhaust
Collar
707 m3 / h (416 cfm)
901 m3 / h (530 cfm)
1114 m3 / h (656 cfm)
1256 m3 / h (739 cfm)
3
3
ULPA Filter Typical Efficiency
Sound Emission
3
<66 dBA
EN 12469
<64.5 dBA
<66 dBA
<67 dBA
<63 dBA
<61.5 dBA
<63 dBA
<64 dBA
> 1400 Lux
(> 130 foot-candles)
> 1070 Lux
(> 100 foot-candles)
> 1225 Lux
(> 114 foot-candles)
Pending
Yes
Yes
Yes
Electrogalvanized steel with Isocide oven-baked epoxy-polyester powder coating
1.2 mm (0.05") /18 gauge
1.5 mm (0.06") /16 gauge
243 kg / 536 lbs
336 kg / 741 lbs
Electrical *
1.2 mm (0.05") /18 gauge
1.5 mm (0.06") /16 gauge
110-130V, AC, 60Hz, 1 ø
Net Weight **
Shipping Weight **
Shipping Dimensions,
Maximum (W x D x H) **
3
> 1230 Lux
(> 114 foot-candles)
NSF / ANSI 49 Certified
Cabinet Construction
3
>99.999% for particle size between 0.1 to 0.3 microns per IEST-RP-CC001.3
NSF / ANSI 49
Fluorescent Lamp Intensity
3
317 kg / 698 lbs
434 kg / 957 lbs
292 kg / 644 lbs
400 kg / 880 lbs
410 kg / 904 lbs
523 kg / 1153 lbs
1230 x 940 x 1900 mm
48.4" x 37" x 74.8"
1530 x 940 x 1900 mm
60.2" x 37" x 74.8"
1910 x 940 x 1900 mm
75.2" x 37" x 74.8"
2150 x 940 x 1980 mm
84.6" x 37" x 77.9"
2.20 m3 (78 cu.ft.)
2.73 m3 (97 cu.ft.)
3.41 m3 (121 cu.ft.)
4.00 m3 (141 cu.ft.)
Shipping Volume, Maximum **
* Additional voltages may be available; contact Esco for ordering information.
** Cabinet only, excludes optional stand.
Accessories and Options (Cont.)
• Cradle stand, electrical hydraulic,
infinitely adjustable, with casters
- Elevates to seating or standing
work surface height.
- When lowered permits movement
through standard doorway.
Note: Increases exterior dimensions.
Electrical Outlets and Utility Fixtures
• Electrical outlet, ground fault,
North America
• Electrical outlet, Europe / Worldwide
• Petcock (air, gas, vacuum)
- North America (American) style
- Europe / Worldwide style DIN 12898,
DIN 12919, DIN 3537
Cabinet Accessories
• Germicidal UV lamp
- Controlled by automatic UV lamp
timer through Sentinel micro-
processor control panel.
- Emission of 253.7 nanometers for
most efficient decontamination.
- Lamp is positioned away from
operator line-of-sight for safety and
proper exposure to interior surfaces.
Note: UV lamp intensity reduces over
time and its effectiveness is subject to
factors such as relative humidity in the
cabinet, ambient air temperature and
microbial species in the work zone.
• PVC armrest
- Chemically treated, improves operator comfort, easy-to-clean. 712 mm
(28") standard size.
Biological Safety Cabinet • Class II, Type A2 Biological Safety Cabinets
• Ergonomic lab chair
- Laboratory grade construction,
meets Class 100 cleanliness; alcohol
resistant PVC materials
- Adjustable 395-490 mm (15.6"-19.3")
• Ergonomic foot rest
- Angled, helps maintain proper
posture.
- Adjustable height
- Anti-skid coating, chemical resistant finish.
• IV bar, with hooks
- Stainless steel construction
- Available for all standard Esco cabinets.
•
-
Microscope viewing device
Mounting and viewing pouch integrated into sash. Factory installed; specify when ordering.
General Specifications
International Models
(Europe, Asia-Pacific, Africa,
Latin America / 230 V, 50 & 60 Hz)
Nominal Size
External Dimensions
(W x D x H)
Gross Internal Dimensions
(W x D x H)
Usable Work Area
Tested Opening
Working Opening
Average Airflow
Velocity
Airflow Volume
LA2-3A1
LA2-3A3
LA2-3J5
LA2-4A1
LA2-4A3
LA2-4J5
LA2-5A1
LA2-5A3
LA2-5J5
LA2-6A1
LA2-6A3
LA2-6J5
0.9 meters ( 3')
1.2 meters ( 4')
1.5 meters ( 5')
1.8 meters ( 6')
1115 x 810 x 1540 mm
43.9" x 31.9" x 60.6"
1420 x 815 x 1540 mm
55.9" x 32.1" x 60.6"
1725 x 815 x 1540 mm
67.9" x 32.1" x 60.6"
2030 x 815 x 1540 mm
79.9" x 32.1" x 60.6"
955 x 623 x 670 mm
37.6" x 24.5" x 26.4"
1260 x 623 x 670 mm
49.6" x 24.5" x 26.4"
1565 x 623 x 670 mm
61.6" x 24.5" x 26.4"
1870 x 623 x 670 mm
73.6" x 24.5" x 26.4"
0.45 m2 (4.8 sq.ft.)
0.6 m2 (6.5 sq.ft.)
0.75 m2 (8.1 sq.ft.)
0.9 m2 (9.7 sq.ft.)
228.6 mm (9")
228.6 mm (9")
228.6 mm (9")
203.2 mm (8")
274 mm (10.8")
274 mm (10.8")
274 mm (10.8")
248 mm (9.8")
Inflow
Downflow
.53 m/s (105 fpm)
0.35 m/s (70 fpm)
0.35 m/s (70 fpm)
0.35 m/s (70 fpm)
0.33 m/s (65 fpm)
Inflow
419 m / h (246 cfm)
552 m / h (325 cfm)
685 m / h (403 cfm)
742 m3 / h (437 cfm)
Downflow, 63%
702 m3 / h (413 cfm)
924 m3 / h (544 cfm)
1147 m3 / h (675 cfm)
1289 m3 / h (758 cfm)
Exhaust, 37%
419 m / h (246 cfm)
552 m / h (325 cfm)
685 m / h (403 cfm)
742 m3 / h (437 cfm)
Required Exhaust
With Optional
Thimble Exhaust
Collar
707 m3 / h (416 cfm)
901 m3 / h (530 cfm)
1114 m3 / h (656 cfm)
1256 m3 / h (739 cfm)
3
3
ULPA Filter Typical Efficiency
Sound Emission
3
3
3
3
>99.999% at particle size between 0.1 to 0.3 microns
NSF / ANSI 49
<66 dBA
<63.5 dBA
<66 dBA
<67 dBA
EN 12469
<63 dBA
<60.5 dBA
<63 dBA
<64 dBA
> 1230 Lux
(> 114 foot-candles)
> 1400 Lux
(> 130 foot-candles)
> 1070 Lux
(> 100 foot-candles)
> 1225 Lux
(> 114 foot-candles)
Fluorescent Lamp Intensity
Electrogalvanized steel with Isocide oven-baked epoxy-polyester powder coating
Cabinet Construction
1.2 mm (0.05") / 18 gauge
220-240V, AC,
50Hz, 1ø
LA2-3A1
LA2-4A1
LA2-5A1
LA2-6A1
220-240V, AC,
60Hz, 1ø
LA2-3A3
LA2-4A3
LA2-5A3
LA2-6A3
100V, AC,
50/ 60Hz, 1ø
LA2-3J5
LA2-4J5
LA2-5J5
LA2-6J5
Net Weight **
243 kg / 536 lbs
283 kg / 624 lbs
317 kg / 698 lbs
350 kg / 772 lbs
Shipping Weight **
292 kg / 644 lbs
345 kg / 761 lbs
410 kg / 904 lbs
486 kg / 1072 lbs
1230 x 940 x 1900 mm
48.4" x 37" x 74.8"
1530 x 940 x 1900 mm
60.2" x 37" x 74.8"
1910 x 940 x 1900 mm
75.2" x 37" x 74.8"
2150 x 940 x 1980 mm
84.6" x 37" x 77.9"
2.20 m3 (78 cu.ft.)
2.73 m3 (97 cu.ft.)
3.41 m3 (121 cu.ft.)
4.00 m3 (141 cu.ft.)
Electrical *
Shipping Dimensions,
Maximum (W x D x H) **
Shipping Volume, Maximum **
* Additional voltages may be available; contact Esco for ordering information.
** Cabinet only, excludes optional stand.
Comprehensive Performance Testing At Esco
Every Labculture LA2 model manufactured by Esco is
individually tested, documented by serial number and
validated with the following test methods.
Inflow / downflow velocity
PAO aerosol challenge for filter integrity
Light, noise and vibration
Airflow pattern visualization
Electrical safety to IEC61010-1
KI-Discus containment
Additional microbiological testing is performed
on statistical sampling basis.
Microbiological Testing
possibly escaping B.atrophaeus spores,
then the sample is incubated
Esco performs testing in accordance
with more than 10 of the world’s most
recognized standards for local, regional
and international criteria. Testing in our
microbiology laboratory is conducted
according to NSF / ANSI 49, EN 12469, and
JIS K3800. An NSF-accredited biohazard
cabinet field certifier is available in-house
full-time to supervise all testing work.
• Acceptance: The number of Bacillus
atrophaeus CFU recovered from the agar
plates shall not exceed 10 CFU per test.
Product Protection Test
The test objective is to determine cabinet
protection to the product/samples inside
the cabinet workzone from environmental
contaminants.
Harmless Bacillus atrophaeus (formerly
Bacillus Subtilis) bacteria is used to
challenge the cabinet, then incubated for
48 hours and the Colony Forming Units
(CFU) are counted to determine the testing
results. Increased microbiological challenge
tests with objects inside the cabinet work
zone, Bunsen burner, external airflow
disturbance, and Human-As-Mannequin
test adapted from Fume Hood development
were performed to simulate real-world
conditions.
• A nebulizer containing 55 mL of 5 to 8
x 106 spores/mL B.atrophaeus is placed
at 10 cm (4 inches) in front of sash
window.
• Target agar plates are placed throughout
the entire work surface.
• Acceptance: The number of Bacillus
atrophaeus CFU recovered from the
agar plates shall not exceed 5 CFU
per test.
Cross Contamination Test
Personnel Protection Test
The test objective is to evaluate cabinet
protection from cross contamination of
samples placed simultaneously inside the
workzone.
The test objective is to evaluate the safety
of the cabinet for the personnel operating
on potentially hazardous samples in the
cabinet workzone.
• A nebulizer containing 55 mL of spores
(5 to 8 x 104/mL) is placed against one
of the workzone sidewalls.
• A nebulizer containing 55 mL of 5 to 8
x 108 spores/mL B.atrophaeus spores is
placed inside the workzone, 10 cm (4
inches) behind the front opening sash.
• Target agar plates are placed 36 cm
(14”) away from the same side wall
• Target slit air samplers and impingers are
placed outside the workzone to capture
• Acceptance: The number of Bacillus
atrophaeus CFU recovered on agar
plates shall not exceed 2 CFU per test.
The Performance Envelope Concept
0.65
Esco cabinets are designed to operate
within a performance envelope to maintain
protection for personnel, product and the
environment.
m/s
High Inflow,
High Downflow
(0.61, 0.43)
0.60
High Inflow,
Low Downflow
(0.58, 0.27)
Airflow parameters used to frame the
performance envelope include both inflow
velocity and downflow velocity.
0.55
The graph illustrates the boundaries of
the performance envelope (based on a
1.2 meter / 4 ft. cabinet), as well as the
nominal performance point at which tests
are conducted.
Nominal
(0.53, 0.35)
Inflow Velocity
10
0.50
The range between high and low Inflow,
and high and low downflow, together
with the fluid dynamics achieved through
sophisticated cabinet design, proportionally
size capture slots, and uniform laminar
airflow, combine to deliver a complete
containment and safety solution expected
of a professional biological safety cabinet.
0.45
0.40
Low Inflow,
Low Downflow
(0.325, 0.25)
0.35
0.20
0.25
Low Inflow,
High Downflow
(0.32, 0.45)
0.30
0.35
0.40
Downflow Velocity
0.45
For details on the Esco performance envelope
contact Esco or your Esco Sales Representative.
0.50
m/s
Biological Safety Cabinet • Class II, Type A2 Biological Safety Cabinets
HPV Test Compliant:
Safer Hydrogen Peroxide
Decontamination Compatibility
Esco biological safety cabinets are
Hydrogen Peroxide Vapor (HPV) compliant
and decontaminatable cabinets approved
by BIOQUELL for this patented process.
HPV is a safer and more efficient alternative
to conventional decontamination using
formaldehyde (CH20):
• HPV is non-carcinogenic and odorless,
while formaldehyde is carcinogenic,
toxic and has pungent smell.
• If there is a gap on the cabinet sealing,
escaping HPV to the lab will decompose
to become oxygen and water. Escaping
formaldehyde, however, is harmful
to people in the lab. Therefore HPV
decontamination can be performed
while people still working inside the lab,
while formaldehyde decontamination
must be performed with no one present
in the lab. The HPV method improves
safety, productivity, and reduces the
time to seal the cabinet.
• HPV biological efficacy is independent
of environmental variables, whereas
formaldehyde efficacy is dependent on
such variables.
• HPV has a better penetration capacity,
resulting in a full decontamination of
the cabinet. The formaldehyde method
is known to result in incomplete
decontamination.
• HPV is more effective and rapid against
biological organisms compared to
formaldehyde.
• HPV requires approximately 4-7 hours for
set-up, decontamination, and tear-down,
compared to a total of 12-15 hours
needed to complete a formaldehyde
decontamination process.
• HPV decontamination effectiveness
is independent of temperature and
humidity. Formaldehyde requires
temperature above 20°C and relative
humidity above 65%.
• For information on the BIOQUELL HPV
methodology contact Esco or your Sales
Representative.
KI Discus Containment
Test According to EN 12469
(Potassium Iodide)
Esco is currently one of the few companies
in the world equipped to perform the KI
Discus test for our customers. The KI Discus
test is defined in the European Standard for
microbiological safety cabinets, EN 12469,
as a test method for validating the operator/
personnel protection capabilities of the
cabinet.
• The KI Discus test shows excellent
correlation with the microbiological
test method for operator protection,
and is useful for validating the actual
containment performance of the
cabinet on-site.
• The KI-Discus takes only 45 minutes as
opposed to 2 days for microbiological
testing.
• Thus, each Esco Labculture LA2 model
is factory tested using the KI Discus
method for operator safety.
Filtration System
6. The cabinet shall have one supply downflow filter
and one exhaust filter. Both filters shall be ULPAtype per IEST-RP-CC001.3 and meet EN1822
(H14) requirements.
7. The filters shall be within an aluminum frame with
mini-pleat design without aluminum separators;
no wood or fiberboard shall be used in the filter
assembly.
8. Typical filter efficiency shall be 99.9997% at
MPPS and 99.999% for 0.1 to 0.3 microns.
9. An integral filter guard shall be affixed to prevent
damage to the filter media.
10.The filters shall be (a) individually scan tested by
the manufacturer, (b) individually scan tested
after assembly, and (b) easily accessible for
scan testing in situ by means of a dedicated
upstream sampling port accessible from within
the cabinet.
11.The supply filter shall be angled and oriented
to the 10° cabinet front angle to maximize
downflow uniformity over the work surface.
12.A removable, perforated metal diffuser shall
be installed below the supply filter to optimize
airflow uniformity and to protect from damage.
Blower System
13.The cabinet shall have a direct drive, permanently
lubricated centrifugal blower/motor dynamically
balanced in two planes compliant to ISO2710 for
low noise, low vibration and long filter life.
14.The blower/motor shall have an external rotor
design and include an automatic thermal cut-out
to disable the motor in case of overheating.
Purchase Specifications
LA2 Series Class II, Type A2 Biological
Safety Cabinet
General Performance and Certifications
1. The biological safety cabinet shall comply with
one or more of the following international
standards, and the manufacturer shall provide a
certified copy of containment and performance
tests equivalent to or greater than specified in the
following independent international standards for
biological safety, electrical and other functional
characteristics: Class II, Type A2 per NSF / ANSI
49 (USA); Class II per EN 12469, JIS K3800, SFDA
YY-0569, SANS12469.
2. The cabinet shall protect (a) the operator and
laboratory environment from particulates
generated within the work zone; (b) the product
and process within the work zone from airborne
contamination from ambient air; (c) and the
product and process within the work zone from
cross contamination.
3. Before shipment each cabinet shall be individually
tested by the KI-Discus test (European Standard
EN12469:2000) to validate operator/personnel
protection. The retention efficiency for the
front aperture shall be not less than 99.999%.
Microbiological testing for cabinet performance
shall be performed on a statistical sampling basis.
4. Each cabinet shall be listed by Underwriters’
Laboratories (UL, CUL) or CE for electrical safety.
5. Original documentation specific to each cabinet
serial number shall be provided with the cabinet
and maintained in the manufacturers’ records.
Test data verifying all performance criteria shall be
available upon request to include: (a) inflow velocity
through direct inflow measurement method; (b)
downflow velocity and uniformity; (c) filter leak
scan with aerosol challenge for both filters; (d)
light, noise, vibration; (e) and electrical safety.
15.The blower/motor shall have an automatic speed
control to compensate for filter loading.
16.The blower/motor system shall be enclosed
within a dynamic chamber shaped steel plenum
and integrated with the removable supply filter
assembly to simplify filter changing.
17.The integral damper shall be externally adjustable.
Cabinet Design, Construction, Cleaning
18.The cabinet shall be of triple wall design whereby
all positive pressure plenums capable of handling
contaminated air shall be surrounded by negative
pressure. No positive pressure areas shall be
accessible external to the cabinet. The third wall
shall conceal utilities.
27.Magnetic, not mechanical, proximity sensors shall
work in conjunction with the control system to
indicate proper sash position for containment.
28.Fluorescent lamps shall be mounted behind the
control panel module out of the work zone.
Electronic ballasts shall be used to eliminate flicker,
extend lamp life and reduce heat output.
29.The UV lamp, if installed, shall be mounted behind
the control panel and away from the user’s line of
site for protection. (Note: Does not apply to 1.8
meter/ 6 ft. model.)
30.The UV lamp shall operate via an automatic
timer with automatic shut-off managed
by the microprocessor controller and shall
be interlocked with the blower/motor and
fluorescent lights for safety.
31. The cabinet shall be designed with a 10° angled
front to optimize user comfort, reduce glare and
maximize reach into the work area.
32.The front grille shall be raised to prevent airflow
blockage and improve comfort.
33.Penetrations for petcocks and service fittings
shall be provided; penetrations shall be offset to
improve user access.
34.The cabinet shall accommodate an optional
mounting stand for fixed-height or adjustableheight configurations.
Control and Alarm System
35.All cabinet functions shall be managed by a
programmable microprocessor control system
capable of software updates via Internet
downloads.
36.The microprocessor controller shall be mounted
on the main control panel facing down toward
the user.
37.The controller shall include soft-touch keypad
controls and backlit LCD displays to permit
operation of the blower/motor, light, UV lamp,
electrical outlet(s) and menu.
38.The controller shall be user programmable in
situ to enable or disable functions such as PIN
(personal identification number) access restriction,
cabinet start-up protocol, airflow alarm and other
microprocessor controlled operations outlined in
the user manual.
39.When programmed ON, the start-up protocol
shall perform an automatic pre-purge and postpurge cycle to ensure proper cabinet operation.
19.The cabinet shall maintain containment
performance even when removable work area
components are removed for cleaning.
40.The controller shall include a blower/motor hours
meter to display aggregate motor running time
to assist in predictive maintenance.
20.The work tray shall be one-piece, removable,
stainless steel with radius corners without crevices
or joints.
41.Audible and visual alarms shall be provided for
unsafe conditions such as improper airflow or
sash position.
21.The cabinet shall have a stainless steel, onepiece fabricated drain trough with open angles
to channel spills to a common drain.
21.Airflow shall be monitored by a temperature
compensating, thermistor-based, true air velocity
sensor mounted in the cabinet.
22.The closed sidewall shall be sealed without
perforations, return air slots or concealed areas
which can contain contaminants.
43.The airflow display and alarm system shall be
individually calibrated before shipment.
23.The cabinet shall be free of sharp edges, nonfunctional protrusions, bolts, screws or hardware,
and all metal edges shall be deburred.
24. The cabinet exterior top shall be slanted to
discourage placement of foreign objects and to
maintain proper exhaust airflow.
Ergonomics and Convenience
25.The front sash shall be frameless to maximize
visibility, and accessible for cleaning front and
back. Sash glass shall be safety glass.
26.The sash counterbalance shall be suspended on
two high-strength cables, and the sash shall lock
into position in the event one cable becomes
detached.
44.The main control panel shall exhibit continuous
display of air velocity and a 24-hour clock display.
Certification, Service and Decontamination
45.The cabinet shall be approved for both hydrogen
peroxide vapor (HPV) and formaldehyde
decontamination protocol.
46.All panels leading to potentially contaminated
and/or hazardous areas shall be color coded red.
47.All components with the exception of blower/
motor and ULPA filters shall be located outside
of contaminated air spaces to facilitate servicing
without the need to decontaminate the cabinet.
48.All exterior surfaces shall be painted with a
permanent antimicrobial inhibitor coating to
minimize contamination.
11
Esco Labculture® Plus
Class II Type A2
Biosafety Cabinet
www.escoglobal.com
Key Features
The Esco Labculture® Plus Class II Type A2
biosafety cabinet is an enhanced version of our
internationally certified Labculture® Class II Type
A2 biosafety cabinet model. Providing operator,
product and environment protection from hazardous
aerosols through superior minipleat ULPA filters , the
product combines the best product performance
features with Esco’s ergonomic design.
The cabinet features Esco Isocide™ antimicrobial
coated external body structure and a reduced
overall height for low-ceiling laboratories.
 Pending LNE/NF certification to EN 12469
 Product / Operator / Environment
Protection
 ULPA Filters with typical efficiency at
>99.999% at 0.12μm provide ISO Class 3
cleanliness within work zone.
 Easy-to-Clean: Single sheet internal walls
 Isocide™ antimicrobial coated structure
eliminates 99.9% of bacterial presence on
external surfaces within 24 hours.
 Ergonomic sloped front eliminates glare
off the front window, and ensures operator
comfort during extended usage
 Microprocessor-based control and alarm
system
 Esco Accuflow™ microprocessor-based motor
speed controller
 Reduced height for low ceiling laboratories
 Extremely low noise level for quiet working
environment
 Laminated shatter-proof sliding sash for
extra protection
Technical Specifications and Engineering Diagram
Esco Catalogue Revision LP2.v1.6.2006
Specifications subject to change without notice
©2006 Esco Micro Pte Ltd
1
2
12
3
4
8
5
670 mm / 26.4”
1436 mm / 56.5”
7
9
13
/ 8.0”
10
203m m
14
6
11
15
555 mm / 21.9”
1204 mm / 47.4”
795 mm / 31.3”
1330 mm / 52.4”
Engineering Details
1. Exhaust ULPA Filter 2. Blower 3. Downflow ULPA filter 4. Standard UV Light Retrofit™ Kit Provision 5. IV Bar Retrofit Kit™ Provision
6. Electrical Outlet Retrofit Kit™ Provision (2 single outlets) 7. Electrical / Electronics Panel 8. Fluorescent Light
9. Plugged Service Fixture Provisions (2 on each side wall) for Gas/Vacuum/Water. (Solenoid Gas Valve Option Available)
10. Stainless Steel Multi-piece Work tray 11. Stainless Steel Armrest
12. Esco Sentinel™ Microprocessor Control System 13. Laminated Shatter-Proof Sliding Sash Window
14. Single-piece Stainless Steel Back Wall and Side Wall 15. Removable Side Panel for Plumbing Access
General Specifications
External Dimensions (L x W x H)
Internal Work Zone (L x W x H)
Standards Compliance
Inflow
Average Airflow
Velocities
Downflow
Airflow Volumes
At Initial Airflow
Velocity Setpoints
Inflow
Downflow (65%)
Exhaust (35%)
Cleanliness Within Working Area
Downflow and Exhaust Filter Type
Filter Efficiency Ratings
LP2-4AX
1330 x 795 x 1486 mm / 52.4” x 31.3” x 56.5”
1204 x 555 x 670 mm / 47.4” x 21.9” x 26.4”
Pending LNE certification to EN12469
Air cleanliness: ISO 14644.1 Class 3, IEST-G-CC1001, IEST-G-CC1002 and other equivalent requirements
Filter performance: IEST-RP-CC034.1, IEST-RP-CC007.1, IEST-RP-CC001.3 and EN1822
Electrical safety: IEC 61010-1 / EN 61010-1 / UL 61010A-1 / CSA C22.2 No. 1010.1-92
Initial setpoint: 0.45 m/s or 90 fpm
Initial setpoint: 0.33 m/s or 65 fpm
Uniformity is better than +/-15%
396 cmh / 233 cfm
772 cmh / 454 cfm
396 cmh / 233 cfm
ISO14644.1 Class 3, US Federal Standard 209E Class 1 / M1.5, AS1386 Class 1.5, JIS B9920 Class 3,
BS5295 Class C, Class M10,000 as per KS27030.1 and equivalent classes of VDI2083 and AFNOR X44101
ULPA filter with integral metal guards and filter frame gaskets; fully compliant with EN 1822 and IEST-RPCC001.3 requirements (each cabinet has individual downflow and exhaust filters)
Typical: 99.9998% at 0.3µm / 99.9998% at 0.12µm / 99.9997% at MPPS
<56.5 dBA
Noise Level
Light Intensity
Main Body Construction
220-240VAC / Cabinet
50Hz 1Ph
Outlet
110-130VAC / Cabinet
60Hz 1Ph
Outlet
Net Weight (Approximate)
Max Shipping Dimensions (L x W x H)
Maximum Power
Consumption /
Current
Max Shipping Volume
The above measurement was taken at initial blower speed setting on a standard 4ft model, as per EN12469
specifications. Figures are subject to acoustic properties of test environment
>1200 Lux; measured at work surface level (zero background) as per NSF49 test grid
1.5mmt / 0.06” / 16 gauge electro-galvanised steel with white oven-baked epoxy anti-microbial Isocide™
antimicrobial powder-coated finish
575W / 4A
1000VA / 5A
765W / 11.5A
500VA / 5A
275 kgs / 606 lbs
1520 x 950 x 1800 mm / 60” x 37.4” x 70.9”
2.6 cbm / 92 cbf
Your Local Distributor:
Esco Biotechnology Equipment Division
21 Changi South Street 1, Singapore 486777
Tel: +65 6543 0833 • Fax: +65 6542 6920
Email: [email protected] • Website: biotech.escoglobal.com
Philadelphia, PA, USA ~ Houston, TX, USA ~ Leiden, The Netherlands ~ Yokohama, Japan ~ Beijing, China ~ Shanghai, China ~ Kuala Lumpur, Malaysia ~ Mumbai, India ~ Dubai, U.A.E.
Esco Labculture® Lead-Shielded
Class II Type A2
Biosafety Cabinet
www.escoglobal.com
Main Features
 Class II Product / Operator / Environment
Protection
 Extra Safety: Cabinet air is filtered via 2 exhaust
filters. This feature allows for filter-changing
without prior decontamination of the cabinet.
 Lead-Shielded Protection: 3mm lead shielding
on cabinet sides, work zone bottom
 Radiation proof glass with lead equivalence of
3mm.
 Horizontal sliding lead glass piece for
Esco Labculture Lead-Shielded Class II
with the standard horizontal sliding lead glass
The Esco Labculture® Lead-Shielded
Class II biosafety cabinet is designed for
radiopharmaceutical industry usage and protects
the operator during work involving radioisotopes.
Superior protection for
radioisotope laboratories
The operator is protected from radiation by the
lead-shielded sides, lead shielded work zone
bottom and front sliding sash. Horizontal sliding
lead glass piece provides additional protection to
the operator during work involving radioisotopes.
The air enters the cabinet through the front air
grille, and travels through the KI-impregnated
carbon filter on which radioactive iodine is
adsorbed. Air then travels to the blower plenum
where it is filtered by high-efficiency ULPA filters
before being exhausted.
additional operator protection.
 KI (potassium iodide) impregnated carbon
filter for iodine adsorption
 ULPA Filters with typical efficiency at >99.999%
at 0.12μm provide ISO Class 3 cleanliness within
work zone.
 Isocide™ antimicrobial coated structure
eliminates 99.9% of bacterial presence on
external surfaces within 24 hours.
 Ergonomic sloped front eliminates glare off
the front window, and ensures operator comfort
during extended usage
 Microprocessor-based control and alarm
system
 Extremely low noise level for quiet working
environment
 Additional options:
 Lead-shielded back panel
 Increased thickness of lead shielding
(standard is 3mm)
Technical Specifications and Engineering Diagram
Esco Catalogue Revision LS2.v2.7.2006
Specifications subject to change without notice
©2006 Esco Micro Pte Ltd
1
7
2
8
4
14
670 mm /26.43”
9
17
16
11
6
760 mm / 30.0”
ACCESS
OPENING
15
248 mm
/9.8”
10
5
2190 mm / 86.2”
13
MAX. OPEN
ING
475 mm
/18.7”
3
12
KNEE SPACE DEPTH
245 mm / 9.6”
750 mm / 29.5”
1420/2030 mm
780 mm / 30.7”
60”/ 80”
1. Exhaust ULPA Filter 2. Blower 3. Downflow ULPA Filter 4. Standard UV Light Retrofit™ Kit Provision
5. Standard IV-Bar Retrofit™ Kit Provision 6. Electrical Outlet Retrofit™ Kit Provision (Two Single Outlets in Workzone)
7. Electrical and Electronics Panel 8. Fluorescent Light 9. Plugged Service Fixture Provisions (2 on each side)
10. Stainless Steel Multi-piece Work Tray 11. Stainless Steel Armrest 12. KI-Impregnated Carbon Filter
13. Esco Sentinel™ Microprocessor Control System 14. Lead-Shielded Glass Sliding Sash Window
15. Stainless Steel Back Wall 16. Lead-Shielded Side Walls 17. Horizontal Lead-Glass Sliding Door
General Specifications
LS2-4AX
LS2-6AX
External Dimensions (L x W x H)
1420 x 780 x 2190 mm / 55.9” x 30.7” x 86.2”
2030 x 780 x 2190 mm / 79.9” x 30.7” x 86.2”
1260 x 603 x 670 mm / 49.6” x 21.3” x 26.4”
1870 x 603 x 670 mm / 73.6” x 21.3” x 26.4”
Internal Work Zone (L x W x H)
Standards Compliance
Average Airflow
Velocities
Airflow Volumes
At Initial
Airflow Velocity
Setpoints
Inflow
Downflow
Designed to comply and exceed the requirements of:
DIN12980: Laboratory Equipment Cytostatic Workbenches: Requirements, Testing
EN12469: Performance Criteria for Microbiological Safety Cabinets
Air cleanliness: ISO 14644.1 Class 3, IEST-G-CC1001, IEST-G-CC1002 and other equivalent requirements
Filter performance: IEST-RP-CC034.1, IEST-RP-CC007.1, IEST-RP-CC001.3 and EN1822
Electrical safety: IEC 61010-1 / EN 61010-1 / UL 61010A-1 / CSA C22.2 No. 1010.1-92
Initial setpoint: 0.45 m/s or 90 fpm (audible / visual alarm will activate at 0.40 m/s or 80fpm)
Initial setpoint range: 0.30 m/s or 60 fpm
Inflow
460 cmh / 270 cfm
680 cmh / 400 cfm
Downflow (65%)
780 - 910 cmh / 460 - 535 cfm
1160 - 1350 cmh / 680 - 794 cfm
Exhaust (35%)
Cleanliness Within Working Area
Exhaust Carbon Filter
460 cmh / 270 cfm
680 cmh / 400 cfm
ISO14644.1 Class 3, US Federal Standard 209E Class 1 / M1.5, AS1386 Class 1.5, JIS B9920 Class
3, BS5295 Class C, Class M10,000 as per KS27030.1 and equivalent classes of VDI2083 and AFNOR
X44101
KI (potassium iodide) impregnated carbon filter (installed directly below the work surface).
ULPA filter with integral metal guards and filter frame gaskets; fully compliant with EN 1822 and IEST-RPCC001.3 requirements. Typical efficiency: 99.9997% at MPPS; 99.9998% at 0.3 and 0.12µm
ULPA filter with integral metal guards and filter frame gaskets; fully compliant with EN 1822 and IEST-RPExhaust ULPA Filter Type
CC001.3 requirements. Typical efficiency: 99.9997% at MPPS; 99.9998% at 0.3 and 0.12µm
According to EN12469
<61 dBA
<65 dBA
Noise Level
The above measurements were taken at initial blower speed setting (figures subject to acoustic properties of test environment)
Light Intensity
>1000 Lux / >93 foot candles (measured at work surface level (zero background) as per NSF49 test grid)
Maximum Usable Workzone Depth
510 mm / 20” (does not include the 110 mm / 4.3” front air grille)
Maximum Usable Work Area (excludes front air
0.64 sqm / 6.89 sqf
0.95 sqm / 10.22 sqf
grille)
Knee Space Depth
245 mm / 9.6”
1.5mmt / 0.06” / 16 gauge electro-galvanised steel with white oven-baked epoxy anti-microbial Isocide™
Main Body Construction
powder-coated finish. 3mm / 0.12” lead shielding on cabinet side walls and work zone bottom
Maximum
220-240VAC / Cabinet
675W / 4A
765W / 4A
Outlet
1000VA / 5A
1000VA / 5A
Power
50Hz 1Ph
835W / 11.5A
1045W / 11.5A
Consumption / 110-130VAC / Cabinet
Outlet
500VA / 5A
500VA / 5A
60Hz 1Ph
Current
Downflow ULPA Filter Type
Your Local Distributor:
Esco Biotechnology Equipment Division
21 Changi South Street 1, Singapore 486777
Tel: +65 6543 0833 • Fax: +65 6542 6920
Email: [email protected] • Website: biotech.escoglobal.com
Philadelphia, PA, USA ~ Houston, TX, USA ~ Leiden, The Netherlands ~ Yokohama, Japan ~ Beijing, China ~ Shanghai, China ~ Kuala Lumpur, Malaysia ~ Mumbai, India ~ Dubai, U.A.E.
Esco Infinity tested to EN 12469 and TUV-GS certified for safety and
performance. Model FC2-4A_, with optional support stand.
Class II Microbiological Safety Cabinet
The Industry's Premier Biological Safety Solution from Esco
Main Features
Unique Dynamic Chamber™ plenum with
angled filter delivers superb airflow uniformity
with deviation no greater than 5% to 6%
of average downflow, exceeding European
Standard EN 12469 by more than three times.
Negative pressure plenum surrounds
contaminated positive pressure plenum;
no fabric bags are used.
Dual fan design for guaranteed safety.
If one fan fails, minimal protection is still
maintained with only one fan running.
Esco next-generation Sentinel™ microprocessor
supervises all cabinet functions.
A large easy-to-read digital display and
ergonomically sized touchpad controls improve user interface.
Fully closable, motorized sash provides
an airtight seal for better safety when
cabinet is inoperative overnight.
Frameless, shatterproof sash is easier to
clean, offers larger, unobstructed viewing area.
Ergonomically angled front improves
reach and comfort.
Multi-piece work surface removal
simplifies cleaning.
Raised armrest maintains safety by
preventing blockage.
Esco
antimicrobial surface on
all painted surfaces minimizes contamination.
Downflow air sensor mounted in interior.
Each cabinet is KI-Discus tested for performance integrity.
Available in 0.9, 1.2, 1.5 and 1.8 meter models (3', 4', 5' and 6').
Shown with optional telescoping stand.
..
Tested to EN 12469 and TUV-GS certified
for safety and performance.
Biological Safety Cabinets • Class II Microbiological Safety Cabinet
Accuflow™ microprocessor speed controller
maintains safe cabinet airflow despite
supply voltage fluctuations.
Independent exhaust sensor mounted
exterior to work area.
Single piece side and back wall
construction.
Integrated RFI and electrical noise filters
eliminate interference with and from
adjacent equipment.
Improved lighting is brighter, more
uniform and reduces glare.
Low noise level <58dBA (1.2 meter (4’)
cabinet) is significantly quieter than conventional cabinets.
Optional UV lamp operates on
programmable timer.
Dual, long-life ULPA (per IEST-RP-CC001.3)
filters for supply and exhaust airflow.
HPV-compliant and approved for safe
decontamination using BIOQUELL
technology.
Enhanced side-capture zones optimize
containment.
The front sash is motorized for convenient one-hand operation. The sash control
is mounted on the front control panel.
Integrated sash proximity contacts sense
proper sash position, serve as an interlock
for the UV lamp, and activate an alarm
if the sash is improperly positioned.
When fully lowered the sash seals automatically against a closed-cell peripheral
gasket to isolate the interior and
prevent escape of contaminants
during decontamination.
Motorized Front Sash Assembly and Sash Seal
The magnetic switch eliminates the chance of mechanical wear and tear typical of a mechanical switch.
The laminated glass maintains containment if the sash is accidentally broken during cabinet operation.
The back of the sash can be easily cleaned by removing the sash track cover to swing up the sash glass.
A graphical interface indicates cabinet performance.
Display language can be user-programmed for English,
German, French, Italian or Spanish.
Enlarged, multi-line digital read-out with alpha-numeric
display indicates all input, status and alarm functions.
Indicators for filter life and
UV life are reverse scaled from
100% to 0% and displayed
on the LCD panel.
Operator, Product and Environmental Protection
Infinity Class II Model FC2-4A_ delivers three times better
downflow uniformity than required by European Standard
EN 12469, and provides protection from airborne contaminants to the operator, product and the environment.
Model FC2-4A_ shown with optional height adjustable
stand with casters and optional adjustable footrest.
When programmed ON • the start-up sequence confirms status with Air Safe and local time display.
• the Personal Identification Number (PIN) access restricts unauthorized adjustments.
• an airflow alarm warns of deviations from normal velocities.
Advanced Engineering
Containment and Protection
The Esco Infinity microbiological safety
cabinet, includes a number of design and
performance features not found on our
popular Labculture® series cabinets. These
include:
The Esco Infinity Class II Microbiological
safety cabinet (FC2) provides operator,
product and environmental protection
against Biosafety Levels 1, 2 and 3. This
cabinet can be used for handling Biosafety
Level 4, provided that the operator wears
positive pressure suit.
• An aerosol tight window for additional
safety while the cabinet is inoperative.
• Double fan design guarantees safety in
the event of the failure of one fan.
• Motorized front sash for one-hand
operation.
• Larger LCD display for easy monitoring
of operational parameters.
• Reduced height permits cabinet installation for seated operation in standard
2.4 meter (nominal, <8') laboratory ceiling height. Cabinet shown on optional
mounting stand with casters, seated
height work surface 711 mm (28").
• The airflow ratio of 65% recirculation to
35% exhaust increases operator protection beyond the 70% / 30% ratio
of conventional microbiological safety
cabinets.
• Inflow of room air enters the front air
grille to establish operator protection;
room air does not enter the work zone,
preventing product contamination.
• Raised armrest prevents the likelihood
of inflow grille blocking by operator's
arms.
Esco Filter Efficiency
Independent supply and exhaust
filters provide 99.999% typical efficiency
for particle sizes of 0.1 to 0.3 microns.
Infinity Series filters meet the IEST-RPCC001.3 recommended practice for
ULPA performance (USA), and EN 1822
for HEPA performance (EU).
Typical Penetration
Biological Safety Cabinets • Class II Microbiological Safety Cabinet
• Auto-purge holes located at the front
side walls eliminate eddy currents and
dead air pockets in the critical area
behind the sash window.
• The downflow (supply) filter is tilted
proportional to the cabinet front angle
to direct more air forward to the front
air grille.
• The inflow velocity, downflow velocity,
and airflow path, and intake geometry
are precision tuned and tested to create
an optimum air curtain on the front
aperture; this curtain maintains personal and product protection even in
the unlikely event of a severe inflow
or downflow imbalance that would
compromise protection in a conventional cabinet.
Integrated Filtration System
Independent supply and exhaust filters
provide 99.999% typical efficiency for
particle sizes of 0.1 to 0.3 microns. Infinity
Series filters meet the IEST-RP-CC001.3
recommended practice for ULPA performance (USA), and EN 1822 for H14
performance (EU).
• ULPA filters (per IEST-RP-CC001.3),
are tested to a typical efficiency of
>99.999% for 0.1 to 0.3 micron particles; these provide better filtration
capability than conventional H13 HEPA
filters that have a typical efficiency of
> 99.99% for 0.3 micron particles.
Color coded indicator lamps display
green for fan operation; blue for fluorescent lights and electrical outlet; and
orange for UV lamp ON caution.
Programmable automatic UV light timer simplifies
operation, enhances contamination control, extends
UV lamp life and saves energy.
Esco Next-Generation Sentinel
Microprocessor Control System
The Sentinel microprocessor-based
control and alarm system supervises all
cabinet functions.
Setpoints and other applications are user
activated through touch-pad programming
access detailed in the Operations Manual.
A key switch located on the cabinet
prevents unauthorized use of the cabinet.
The motorized sash is controlled by an
up/down button.
A data output for remote monitoring and
information management to meet
FDA21CFR and other criteria is available;
contact Esco or your Sales Representative
for details.
Enlarged touchpad data entry buttons with tactile feedback permit control settings and access
to diagnostics, default settings and hierarchical menus.
Mini-pleat Separatorless Filter (left) vs.
Conventional Aluminium Separator Filter (right)
Sentinel Microprocessor Control,
Alarm, Monitoring System
The Esco Sentinel microprocessor-based
control system supervises operation of all
cabinet functions.
• Continuous monitoring of cabinet airflow is displayed on a bright, easy-to-read
LCD panel. The large display monitors
operational parameters.
• Filter assembly is constructed in accordance with EN1822 requirements.
• The supply filter provides ISO Class 3
(per ISO14644.1) clean air to the work
surface in a gentle vertical laminar flow
for product protection.
• Modern separatorless mini-pleat filter
construction maximizes the filter surface
area to extend filter life and eliminate
possible filter media damage by thin
and sharp aluminum separators used in
conventional HEPA filter construction.
• The exhaust filter traps biohazard particles acquired from the work surface
before air is exhausted to the room,
offering personal and environmental
protection.
• The exhaust filter media is protected
from mechanical damage by an integrated metal screen guard, which is
absent from conventional HEPA filters.
Input Voltage
• The control panel is located on the center
of the cabinet, and angled down for easy
access by the operator.
• A back-up battery maintains alarm
function for airflow, power and pressure
alarm in the event of a power failure.
• Two integrated, temperature-compensated true airflow velocity sensors
provide independent measurement of
inflow and downflow velocities despite
room temperature fluctuation.
• A one-touch half-speed setting can be
activated at the end of the work day to
reduce energy consumption, prolong
filter life and maintain interior work
zone cleanliness for the next day's
work.
• All electronic parts are contained inside
a plug-and-play module that permits
easy exchange if required.
• Microprocessor software updates are
available from Esco for download via
the Internet.
• Sentinel functions are factory set to
default to ON or OFF, depending on
worldwide destination and local preferences. Default settings can be user
activated through the touchpad data
entry access.
Esco Accuflow Microprocessor Speed Controller
Input Voltage
Esco cabinets use Swedish Camfil Farr® mini-pleat
filters without aluminum separators to increase filter
efficiency, minimize the chance of leakage, and to
prolong filter life. Filters include a lightweight aluminum frame for structural stability and elimination of
swelling common to conventional wood frames.
• A pressure sensor monitors pressure
drop across the filter plenum and computes estimated remaining filter life.
Blower Speed
The Esco Accuflow™ microprocessor speed controller maintains steady motor/blower speed despite
building voltage fluctuations, thereby assuring constant face velocity and downflow for optimum safety,
containment and protection.
The Esco Accuflow™ microprocessor speed controller maintains
steady blower motor speed despite building voltage fluctuations,
thereby assuring constant face velocity and downflowfor optimum
safety, containment and protection.
Blower S
Cabinet Filtration System
Side capture zones
Dynamic air barrier, inflow and
forward-directed downflow air converge
Ambient air is pulled through the perforations located towards the work zone front
to prevent contamination of the work surface and work product. The inflow does
not mix with the clean air within the cabinet work zone. Inflow air travels through
a return path toward the common air
plenum (blower plenum) at the top of the
cabinet.
ULPA-filtered air
Unfiltered / potentially contaminated air
Room air / Inflow air
• Automatic start-up sequence will prepare the cabinet for normal operation
and advise when safe conditions are
established.
Approximately 35% of the air in the
common plenum is exhausted through
the ULPA filter to the room. The remaining 65% of the air is passed through the
downflow ULPA filter and into the work
area as a vertical laminar flow air stream
bathing the work surface in clean air.
The uniform, non-turbulent air stream
protects against cross contamination
within and throughout the work area.
• Dual permanently lubricated direct-drive
external rotor motor/blowers safety in
the event of a motor failure.
• An administrator controlled PIN (Personal Identification Number) can be set
to restrict access to main menu.
• The external rotor motor design allows
for optimum cooling of the motor
during extended operations and extends the motor bearing life.
• The airflow alarm can be activated or
deactivated depending on user preference and nature of the work.
• The inflow and downflow balance is
precisely established by two independent Accuflow fan speed controls.
Consult your Esco Operating Manual or
contact Esco or your Sales Representative
for information on user-preference programming capabilities built into the Sentinel
microprocessor platform.
• The Accuflow microprocessor based
speed controller maintains constant,
stable airflow despite building supply
voltage fluctuations.
Redundant Blower System
The Infinity blower system is designed for
high performance operation, redundancy,
maximum energy efficiency and minimal
maintenance.
• Speed can be adjusted electronically
without mechanical adjustment.
• Built-in RFI and electrical noise filters
eliminate interference with adjacent
instrumentation.
• An integral blower hour meter tracks
Esco Centrifugal Fan with External Rotor Motor (left) vs.
Conventional Fan with Standard Motor (right)
Esco Infinity cabinets use a combination of high
performance scroll blowers (supply) and German
made ebm-papst® permanently lubricated, centrifugal
motor/blowers with external rotor designs (exhaust).
Selected for energy efficiency, compact design,
and flat profile, the completely integrated exhaust
blower assembly optimizes motor cooling, with
unified rotating parts and overall component balance
for smooth, quiet, vibration-free operation. Weight
is equally distributed to all bearings to extend bearing
life, transfer heat and maximize speed control.
Biological Safety Cabinets • Class II Microbiological Safety Cabinet
Near the work surface, the downflow
air stream splits with a portion moving
toward the front air grille, and the remainder moving to the rear air grille. A small
portion of the ULPA filtered downflow
enters the intake perforations at the side
capture zones at a higher velocity (small
blue arrows).
A combination of inflow and downflow
air streams forms an air barrier that prevents contaminated room air from entering the work zone, and prevents work
surface emissions from escaping the work
zone.
Air returns to the common air plenum
where the 35% exhaust and 65% recirculation process is continued.
operating life and aids in predictive maintenance planning.
• To prevent fan damage, a paper-catch
grille traps papers or towels that may
drop down on the drain pan, preventing
them from being pulled into the column
by fan suction.
Cabinet Construction
Robust construction and enhanced safety
features qualify the cabinet for the most
demanding laboratory applications. The
cabinet is fully assembled and ready to
install and operate when shipped.
• The interior sides and back wall are
formed from a single piece of stainlesssteel with large radius corners to simplify interior cleaning.
• The cabinet work zone has no welded
joints to collect contaminants or rust.
• All stainless steel work surfaces are
accessible for cleaning.
• Multi-piece tray components are easily
lifted and removed to encourage surface decontamination.
• A recessed central area and stainless
steel drain pan channels spills and
prevent liquids from entering the lower
filtration and blower systems.
Double Blower System
Provides the maximum possible level of safety by enabling safe cabinet shut down in the event of a single blower failure.
AIRAIR
SAFE
AIR
SAFE
SAFE
SUPPLY
FAIL
SUPPLY
SUPPLY
FAILFAIL
EXHAUST
FAIL
EXHAUST
EXHAUST
FAILFAIL
Downflow
Fail
Downflow
Downflow
FailFail
1A: Under normal operation with both blowers operating (1a) the supply blower creates a negative
pressure surrounding the contaminated positive pressure plenum and pushes air across the supply and exhaust filters. The exhaust blower boosts the air pressure through the exhaust filter to create better inflow and operator protection. Supply and exhaust blowers automatically operating at
reduced speeds extend blower life.
Dynamic Chamber™ Plenum Design
1B: If the supply blower fails (1b), downflow to
the work area is suspended. The exhaust blower
ramps up to maximum power to provide inflow
to the cabinet to help maintain containment. The
control panel warns of downflow failure. With the supply blower offline, the cabinet still provides protection by maintaining inflow above 0.40 m/s as required by EN 12469.
1C: If the exhaust blower fails (1c), the supply
blower continues to provide inflow to the cabinet
and downflow to the work area. The control panel warns of inflow failure. With the exhaust
blower offline, the cabinet maintains protection by maintaining inflow above 0.40 m/s as required by EN 12469.
• The External plumbing is concealed
behind trim panels to preserve cabinet
aesthetics.
• The sliding window can be fully opened
to insert and remove larger instrumentation and equipment.
• A normally closed gas solenoid valve
automatically shuts off gas flow in
the event of a cabinet alarm or unsafe
condition.
Comfortable Ergonomic Design
The cabinet is engineered for comfort,
utility value and safety.
Negative pressure
Positive pressure
The Esco triple-wall design creates a Dynamic Chamber plenum which surrounds contaminated areas
with negative pressure, preventing the possibility
of contamination from leaks in filter seal, gasket or
cabinet structure. The third wall conceals utilities.
• External surfaces are coated with Esco
Isocide antimicrobial coating to protect
against surface contamination and inhibit bacterial growth. Isocide eliminates
99.9% of surface bacteria within 24
hours of exposure.
• There are no screws in the front or sides
to trap contaminants or complicate
cleaning.
Service Fitting Access
The cabinet is pre-plumbed for easy installation optional gas and vacuum fittings;
see Accessories.
• The Service fitting openings are offset for
easier access.
Inflow
Fail
Inflow
Inflow
FailFail
• The angled viewing window and narrow
profile front grille improves reach into
the work area.
• The instant-start 5000k fluorescent
lamp operates on an electronic ballast
to reduce heat, improve comfort and
conserve energy.
• The lamp delivers uniform lighting to
the work surface for greater comfort,
reduced glare and improved productivity;
see Specifications.
• The front armrest is raised above the
work zone to improve comfort and to
minimize blockage of forward airflow
perforations.
• The optional adjustable support stand
provides work surface height control.
• The frameless sash eliminates operator's
line of sight blockage.
• A generous sash opening allows for
easier access into the work zone, provides
ample room for transferring of small equipment; see Specifications.
Electrical Safety and Certification
All components meet or exceed applicable
safety requirements.
• Each cabinet is individually factory
tested for electrical safety.
• Documentation specific to each cabinet
serial number is maintained on file.
• Certified to the renowned world standard for microbiological safety cabinets;
the TUV GS mark for compliance, to
EN 12469 (May, 2007).
• Contact Esco or your Sales Representative
for site preparation information; see
Electrical Specifications.
Warranty
The Infinity is warranted for 3 years excluding consumable parts and accessories.
• Each cabinet is shipped with a comprehensive User's Manual complete with a
report documenting all test procedures.
• Additional IQ/OQ/PQ documentation is
available upon request.
• Contact your local Sales Representative
for specific warranty details or documentation requests.
Standards
Compliance
Microbiological
Safety Cabinets*
Air Quality
Filtration
Certified, TUV-GS
to EN 12469:2000
(May, 2007)
ISO 14644.1 Class 3, Worldwide
AS 1386 Class 1.5, Australia
JIS B9920 Class 3, Japan
BS 5295 Class C, UK
KS27030.1, Class M10,000, Korea
IEST-G-CC1001, USA
IEST-G-CC1002, USA
IEST-RP-CC034.1, USA
IEST-RP-CC007.1, USA
IEST-RP-CC001.3, USA
EN 1822, Europe
Electrical Safety
IEC 61010-1, Worldwide
EN 61010-1, Europe
UL 61010A-1, USA
CSA C22.2 No. 1010.1-92, Canada
*Models FC2-4A1 and FC2-6A1. Certification for additional models is pending or in process.
For details on most recent certifications contact Esco or your Sales Representative.
Accessories and Options
Esco offers a variety of options and accessories to meet local applications. Contact
Esco or your local Sales Representative for
ordering information.
Perforated Work Tray
Available for applications where a perforated work surface is preferred. Must
be specified when ordering.
Electrical Outlets and Utility Fittings
• Electrical outlet, ground fault,
North America
• Electrical outlet, Euro/Worldwide
•
Petcock (air, gas, vacuum)
- North America (American) style
- Euro/Worldwide style DIN 12898,
DIN 12919, DIN 3537
Support Stands
• Fixed height, available 737 mm (29")
or 838 mm (33"),
±38.1 mm (1.5")
- With leveling feet
- With casters
•
Adjustable height, hydraulic range
from 737 mm (29") to 838 mm (33")
- Manual or electrical lift
- With casters
•
Telescoping height, nominal range
from 737 mm (29") to 838 mm (33")
- Adjustable in 25.4 mm
(1") increments
•
Infinitely adjustable cradle stand,
with casters
- Elevates to seating or standing
work surface height
- When lowered permits movement
through standard doorway
Note: Increases exterior dimensions.
Cabinet Accessories
• PVC armrest
- Chemically treated, improves
operator comfort, easy-to-clean,
712 mm (28") standard size
•
Ergonomic lab chair
- Laboratory grade construction,
meets Class 100 cleanliness; alcohol
resistant PVC materials
- Adjustable 395-490 mm (15.6"-19.3")
•
Germicidal UV lamp
- Controlled by automatic UV lamp
timer through Sentinel microprocessor
control panel
- Emission of 253.7 nanometers for
most efficient decontamination
- Lamp is positioned away from
operator line-of-sight for safety and
proper exposure to interior surfaces
Note: UV lamp intensity reduces over time
and its effectiveness is subject to factors such
as relative humidity in the cabinet, ambient
air temperature and microbial species in
the work zone.
•
Ergonomic foot rest
- Angled, helps maintain
proper posture
- Adjustable height
- Anti-skid coating, chemical
resistant finish
• IV Bar, with hooks
- Stainless steel construction
- Available for all standard cabinets
•
Microscope viewing device
- Mounting and viewing pouch
integrated into sash
- Factory installed; specify when
ordering
Robust Cabinet Construction and Enhanced Safety Features
Service fixtures are offset for easier reach. Standard cabinets include two fixture provisions on each
sidewall (one provision on each sidewall for .9 meter/3’ cabinet). Electrical outlets are mounted below
service fixtures to minimize obstructions.
The one piece stainless steel side wall is radiused on the corner and easy to clean without crevices or joints.
Helpful for certifiers, the hinged maintenance assembly opens to a fixed position on integrated,
gas spring struts providing front service access.
All key components with the exception of the motor/blower assembly are mounted outside the air stream and
away from contaminated air to permit service without decontamination. These include fluorescent lamps, UV
lamp, electrical harnesses, electronic boards and microprocessor control.
Panels enclosing potentially hazardous areas or components such as microbiological contamination
or electrical shock are color-coded red to warn service technician.
The telescoping Dynamic Chamber™ plenum minimizes physical lifting and accelerates filter change when
required.
Work area containment is maintained even when removable components are lifted out for cleaning.
The lower drain trough is a single-piece fabrication with wide open angles and a channel
to direct spills to the drain.
Biological Safety Cabinets • Class II Microbiological Safety Cabinet
Model FC2 Biological Safety Cabinet Technical Specifications
19
1
76
1
87
6
27
3
49
109
11
16
ACC S
OPENES
ING
12
17
18
5
13
955 mm / 1260 mm / 1565 mm / 1870 mm
(37.6"/ 49.6"/ 61.6"/ 73.6")
1. Exhaust filter
7. Exhaust fan
2. Blower
8. Electrical / Electronics panel
3. Downflow filter
9. Fluorescent light
4. Standard UV light Retrofit
Kit™ provision
10.Gas service fixture with
solenoid valve
5. Standard IV-Bar Retrofit Kit
provision
11.Vacuum service fixture
6. Universal electrical outlet
(0.9 meter / 3' model - one
single outlet in work zone)
(1.2, 1.5 and 1.8 meter /
4', 5' & 6' models - two
single outlets in work zone)
12.Stainless steel single-piece
work tray
13.Stainless steel armrest
14.Key switch
805 mm (31.7")
15.Esco Sentinel microprocessor
control system
16.Motorized sliding sash
(aerosol tight)
17.Single piece stainless steel
back wall and side wall
18.Side removable panel for
plumbing access
*Access Opening Height
All Model Sizes
Testing Opening Height
200 mm (7.9")
Working Area Height
210 mm (8.3")
*The combination of the Esco raised armrest and recessed
work surface creates additional space within the working area
than typically specified.
19.Thimble exhaust collar
(optional)
Optional Exhaust Collar Positions for Thimble-Ducting for FC2 Models
460 mm
(18.1")
190 mm
(7.5")
262 mm
(10.3")
250 mm
(9.8")
Top View - FC2-4A_
262 mm
(10.3")
1015 mm
(40.0")
Side View - All Sizes
6
610 mm (24")
1115 mm / 1420 mm / 1725 mm / 2030 mm
(43.9"/ 55.9"/ 67.9"/ 79.9")
670 mm (26.4")
14
1540 mm (60.6")
15
9
Top View - FC2-6A_
General Specifications
FC2-3A_
FC2-4A_
FC2-5A_
FC2-6A_
0.9 meters ( 3')
1.2 meters ( 4')
1.5 meters ( 5')
1.8 meters ( 6')
External Dimensions
(W x D x H)
1115 x 805 x 1535 mm
43.9" x 31.7" x 60.4"
1420 x 805 x 1535 mm
56.0" x 31.7" x 60.4"
1725 x 805 x 1535 mm
67.9" x 31.7" x 60.4"
2030 x 805 x 1535 mm
80.0" x 31.7" x 60.4"
Internal Work Area Dimensions
(W x D x H)
955 x 610 x 670 mm
37.6" x 24.0" x 26.4"
1260 x 610 x 670 mm
49.6" x 24.0" x 26.4"
1565 x 610 x 670 mm
61.6" x 24.0" x 26.4"
1870 x 610 x 670 mm
73.6" x 24.0" x 26.4"
0.47 m2 (5.06 sq.ft.)
0.62 m2 (6.67 sq.ft.)
0.77 m2 (8.29 sq.ft.)
0.92 m2 (9.90 sq.ft.)
Tested Opening
200 mm (7.9")
200 mm (7.9")
200 mm (7.9")
200 mm (7.9")
Working Opening
210 mm (8.3")
210 mm (8.3")
210 mm (8.3")
210 mm (8.3")
Nominal Size
Internal Work Space Area
Power Rating
Code1
Average Airflow
Velocity
1, 3
0.48 m/s (95 fpm) at initial setpoint
2
0.53m/s (105 fpm) at initial setpoint
1, 2, 3
0.35m/s (70 fpm) at initial setpoint
Inflow
Downflow
1, 3
332 m3 / h (195 cfm)
437 m3 / h (257 cfm)
593 m3 / h (319 cfm)
646 m3 / h (380 cfm)
2
336 m3 / h (216 cfm)
483 m3 / h (284 cfm)
599 m3 / h (353 cfm)
714 m3 / h (420 cfm)
1, 2, 3
702 m3 / h (413 cfm)
929 m3 / h (544 cfm)
1147 m3 / h (675 cfm)
1367 m3 / h (804 cfm)
1, 3
332 m3 / h (195 cfm)
437 m3 / h (257 cfm)
543 m3 / h (319 cfm)
646 m3 / h (380 cfm)
2
336 m3 / h (216 cfm)
483 m3 / h (284 cfm)
599 m3 / h (353 cfm)
714 m3 / h (420 cfm)
1, 3
<60 dBA
<61 dBA
<63 dBA
<64 dBA
2
<61 dBA
<62 dBA
<64 dBA
<65 dBA
1, 3
<57 dBA
<58 dBA
<60 dBA
<61 dBA
2
<58 dBA
<59 dBA
<61 dBA
<62 dBA
Inflow
Airflow Volume
Downflow
Exhaust
NSF 49
10
Sound Emission
(Typical)
EN 12469
ULPA Filter
Typical Efficiency
Downflow
>99.999% at 0.1 to 0.3 microns as per IEST-RP-CC001.3 USA
>99.995% at MPPS as per EN 1822 (H-14) EU
Exhaust
Fluorescent Lamp Intensity
Cabinet
Construction
> 1200 Lux (111 foot-candles)
Main Body
1.2 mm (0.06") 16 gauge electrogalvanized steel with white oven-baked epoxy
Isocide antimicrobial powder coated finish
Work Zone
Stainless steel grade 304; Work tray: 1.2 mm (0.04"); Walls and drain pan: 0.9 mm (0.03")
Side Walls
Stainless steel grade 304
220-240V, AC, 50Hz, 1ø
A1
FC2-3A1
FC2-4A1
FC2-5A1
FC2-6A1
110-130V, AC, 60Hz, 1ø
A2
FC2-3A2
FC2-4A2
FC2-5A2
FC2-6A2
220-240V, AC, 60Hz, 1ø
A3
FC2-3A3
FC2-4A3
FC2-5A3
FC2-6A3
Net Weight **
228 kg / 503 lbs
298 kg / 657 lbs
372 kg / 820 lbs
447 kg / 985 lbs
Shipping Weight **
280 kg / 617 lbs
354 kg / 780 lbs
443 kg / 977 lbs
522 kg / 1150 lbs
1200 x 900 x 1870 mm
47.2" x 35.4" x 73.6"
1530 x 900 x 1870 mm
60.2" x 35.4" x 73.6"
1910 x 900 x 1870 mm
75.2" x 35.4" x 73.6"
2150 x 900 x 1870 mm
84.6" x 35.4" x 73.6"
2.02 m3 (71 cu.ft.)
2.58 m3 (91 cu.ft.)
3.22 m3 (114 cu.ft.)
3.62 m3 (128 cu.ft.)
Electrical *
Shipping Dimensions,
Maximum (W x D x H) **
Shipping Volume, Maximum **
* Additional voltages may be available; contact Esco for ordering information.
** Cabinet only, excludes optional stand.
1
AC power specifications are to be specified in the last digit of the model number when ordering: 1, 220 240V, 50Hz; 2, 110-130V 60Hz; 3, 220-240V, 60Hz.
Note: 60 Hz models meet or exceed NSF 49 standards for safety and performance. For information contact Esco or your Sales Representative.
Biological Safety Cabinets • Class II Microbiological Safety Cabinet
Microbiological Testing
Esco performs testing in accordance with more
than 10 of the world's most recognized
standards for local, regional and international criteria. Testing in our micro-biology
laboratory is conducted according to NSF49,
EN12469, and JIS K3800. An NSF-accredited
biohazard cabinet field certifier is available
in-house full-time to supervise all testing
work, using harmless Bacillus atrophaeus (formerly Bacillus Subtilis) bacteria that is used
to challenge the cabinet, then incubated
for 48 hours and the Colony Forming Units
(CFU) are counted to determine the testing results. Increased microbiological challenge tests with objects inside the cabinet
work zone, Bunsen burner, external airflow
disturbance, and Human-As-Mannequin test
adapted from Fume Hood development were
performed to simulate real-world conditions.
Personnel Protection Test
The test objective is to evaluate the safety of
the cabinet for the personnel operating on
potentially hazardous samples in the cabinet
work zone.
• A nebulizer containing 55 mL of 5 to 8 x 10
spores/mL B.atrophaeus spores is placed
inside the work zone, 10 cm (4") behind the
front opening sash.
8
• Target slit air samplers and impingers are
placed outside the work zone to capture
possibly escaping B.atrophaeus spores, then
the sample is incubated.
• Acceptance: The number of Bacillus atrophaeus CFU recovered from the agar plates
shall not exceed 10 CFU per test.
Product Protection Test
The test objective is to determine cabinet protection to the product/samples inside the cabinet
work zone from environmental contaminants.
Cross Contamination Test
The test objective is to evaluate cabinet protection from cross contamination of samples
placed simultaneously inside the work zone.
• A nebulizer containing 55 mL of 5 to 8 x 104
spores/mL is placed against one of the work
zone sidewalls.
• HPV requires approximately 4-7 hours for
set-up, decontamination, and tear-down,
compared to a total of 12-15 hours needed
to complete a formaldehyde decontamination process.
• Target agar plates are placed 360 mm (14")
away from the same side wall.
• HPV decontamination effectiveness is independent of temperature and humidity.
Formaldehyde requires temperature above
20°C and relative humidity above 65%.
• Acceptance: The number of Bacillus atrophaeus CFU recovered on agar plates shall
not exceed 2 CFU per test.
• For information on the BIOQUELL HPV
methodology contact Esco or your Sales
Representative.
HPV Test Compliant:
Safer Hydrogen Peroxide
Decontamination Compatibility
KI-Discus Containment Test
According to EN 12469:2000
(Operator Protection)
Esco biological safety cabinets are Hydrogen
Peroxide Vapor (HPV) compliant and decontaminatable cabinets approved by BIOQUELL
for this patented process. HPV is a safer and
more efficient alternative to conventional
decontamination using formaldehyde (CH20):
Esco is currently one of the few companies in
the world equipped to perform the KI-Discus
test for our customers. The KI-Discus test is defined in the European Standard for microbiological safety cabinets, EN12469:2000, as a test
method for validating the operator/personnel
protection capabilities of the cabinet.
• HPV is non-carcinogenic and odorless, while
formaldehyde is carcinogenic, toxic and has
pungent smell.
• If there is a gap on the cabinet sealing,
escaping HPV to the lab will decompose to
become oxygen and water. Escaping formaldehyde, however, is harmful to people in
the lab. Therefore HPV decontamination
can be performed while people are working inside the lab, while formaldehyde
decontamination must be performed with
no one present in the lab. The HPV method
improves safety, productivity, and reduces
the time to seal the cabinet.
• The KI-Discus test shows excellent correlation with the microbiological test method
for operator protection, and is useful for
validating the actual containment performance of the cabinet on-site.
• The KI-Discus takes only 45 minutes as
opposed to 2 days for microbiological
testing.
• Thus, each Esco Infinity FC2 model is factory tested using the KI-Discus method for
operator safety.
• HPV biological efficacy is independent of
environmental variables, whereas formaldehyde efficacy is dependent on such
variables.
• A nebulizer containing 55 mL of 5 to 8 x 106
spores/mL B.atrophaeus is placed at 10 cm
(4") in front of sash window.
• HPV has a better penetration capacity,
resulting in a full decontamination of the
cabinet. The formaldehyde method is known
to result in incomplete decontamination.
• Target agar plates are placed throughout
the entire work surface.
• HPV is more effective and rapid against biological organisms compared to formaldehyde.
• Acceptance: The number of Bacillus atrophaeus CFU recovered from the agar plates
shall not exceed 5 CFU per test.
Esco builds quality from the inside out.
Comprehensive Performance Testing At Esco
Every Infinity model manufactured by Esco is individually
tested, documented by serial number and validated with
the following test methods.
Inflow/downflow velocity
PAO Aerosol challenge for filter integrity
Light, noise and vibration
Airflow pattern visualization
Electrical safety to IEC61010-1
KI-Discus containment
Additional microbiological testing is performed
on statistical sampling basis.
11
Labculture Class II, Type B2
Biosafety Cabinet, Model LB2-4B_.
Class II, Type B2 (Total Exhaust) Biological Safety Cabinets
The Safety Solution for Life Science Laboratories
Main Features
Unique Esco Dynamic Chamber™ plenum.
Negative pressure plenum surrounds supply positive
pressure plenum; no fabric bags are used.
Long-life supply ULPA filter (per IEST-RP-CC001.3)
with >99.999% efficiency for particle size between
0.1 to 0.3 microns.
Angled supply filter matches cabinet profile to
achieve best downflow uniformity.
Esco Sentinel™ microprocessor.
Frameless, shatterproof sash is easier to clean.
Ergonomically angled front improves reach and comfort.
Actual work access height is 45 mm (1.8”) higher than
tested sash opening to provide additional work space.
Removable one-piece work surface simplifies cleaning.
Raised airflow grille maintains safety by preventing
blockage.
Optional UV lamp is located behind control
panel away from line of sight.
(Does not apply to 1.8 m / 6' models.)
Esco
antimicrobial coating on all
painted surfaces minimizes contamination.
Enhanced side-capture zones optimize
containment.
Work area on Esco 0.9 meter (3') cabinet is
equivalent to work area offered on larger 1.2 meter (4') conventional cabinet.
Each cabinet is KI-Discus tested for performance integrity.
Available in 0.9, 1.2, 1.5 and 1.8 meter models (3', 4', 5' and 6').
Shown with optional telescoping stand.
Esco Labculture has passed more performance
tests in more languages, for more certifications
throughout more countries than any other
biological safety cabinet in the world.
Biological Safety Cabinet • Class II, Type B2 (Total Exhaust) Biological Safety Cabinets
spills in the work zone from entering
the side air column.
• The ULPA downflow (supply) filter is
tilted proportional to the cabinet front
angle to direct more air forward to the
front air grille.
• The inflow velocity, downflow velocity,
and air flow path, and intake geometry
are precision tuned and tested to
create an optimum air curtain on the
front aperture; this curtain maintains
personal and product protection
even in the unlikely event of a severe
inflow or downflow imbalance that
would compromise protection in a
conventional cabinet.
Integrated Filtration System
Operator, Product and Environmental Protection
The Esco Labculture Class II, Type B2 Biosafety Cabinet
provides operator, product and environmental protection
against Biosafety Levels 1, 2 and 3. This cabinet can be
used for handling Biohazard Level 4, provided that the
operator wears positive pressure suit.
A combination of a supply ULPA filter and
an exhaust HEPA filter give the Labculture
cabinet a fully integrated performance
envelope for product, operator and
environmental protection.
• The total exhaust design of the LB2 makes
the cabinet suitable for microbiological
work involving trace amounts of toxic
chemicals.
• ULPA supply filter (per IEST-RPCC001.3), is tested to a typical efficiency
of >99.999% for 0.1 to 0.3 micron
particles; providing better filtration
capability than conventional HEPA
filters that have a typical efficiency of
> 99.99% for 0.3 micron particles.
• The non-recirculating design guarantees
safety in cases where exhaust HEPAfiltration fails to ensure containment.
• HEPA exhaust filter (per IEST-RPCC001.3), is tested to a typical efficiency
of >99.99% for 0.3 micron particles.
Containment and Protection
• Inflow of room air enters the front air
grille to establish operator protection;
room air does not enter the work zone,
preventing product contamination.
• The front grille has proportionally larger
perforation on the extreme left and
right side. Combined with the side air
gap between the work tray and the side
wall, the stronger side capture zones
increase protection in this critical area
where contaminants tend to bleed out
in conventional cabinet.
• The inflow grille has a convex shape
to maintain operator protection by
discouraging users from placing objects
on it.
• Raised armrest prevents the likelihood of
inflow grille blocking by operator’s arms.
• Auto-purge holes located at the front
side walls eliminate eddy currents and
dead air pockets in the critical area
behind the sash window. Per NSF / ANSI
49 requirement, these side perforations
do not extend completely to the work
surface to prevent accidental liquid
Mini-pleat Separatorless Filter (left) vs.
Conventional Aluminium Separator Filter (right)
Esco cabinets use Swedish Camfil Farr® mini-pleat
filters without aluminum separators to increase filter
efficiency, minimize the chance of leakage, and to
prolong filter life. Filters include a lightweight
aluminum frame for structural stability and
elimination of swelling common to conventional
wood frames.
• Modern separator-less mini-pleat filter
construction maximizes the filter surface
area to extend filter life and eliminate
possible filter media damage by thin
and sharp aluminum separators used in
conventional HEPA filter construction.
• The filter frame and media is constructed
in accordance with EN1822 requirements
for fire retardant properties
• The supply ULPA filter provides ISO Class
3 (per ISO14644.1) clean air to the work
surface in a gentle vertical laminar flow
for product protection.
• The exhaust HEPA filter traps biohazard
particles acquired from the work surface
before air is exhausted via the ducting
system to the external environment. The
Model LB2 is provided with an integral
exhaust collar for connecting the cabinet
exhaust to an airtight ducting system.
NB: Ductworks are not provided with
standard product.
Front Sash Assembly
• Integrated sash proximity contacts sense
sash position, serve as an interlock for
the UV lamp, and activate an alarm if
the sash is improperly positioned.
• The magnetic switch eliminates the
chance of mechanical wear and tear
typical of a mechanical switch.
• The back of the sash can be easily
cleaned by removing the sash track
cover and swing up the sash glass.
• The sash is counterbalanced for smooth
and light sash moving operation.
• The counterbalance locking mechanism
is inherently safe; it locks the counterbalance in place if either of the 2 cables
is detached. The sash cable and cable
clip have rated strength of more than
6x the weight of the sash window of
the largest Labculture Class II cabinet
(1.8 meter/6 ft model)
• The safety glass maintains containment
if the sash is accidentally broken during
cabinet operation.
Esco ULPA Filter Efficiency
Typical Penetration
Esco cabinets use ULPA filters (per
IEST-RP-CC001.3) instead of conventional
HEPA filters commonly found in
biological safety cabinets. While HEPA
filters offer 99.99% typical efficiency
at 0.3 micron level, ULPA filters provide
99.999% typical efficiency for particle
sizes of 0.1 to 0.3 micron level.
Cabinet Filtration System
Side capture zones
Dynamic air barrier, inflow and
forward-directed downflow air converge
Ambient air is pulled through the front
grille to prevent contamination of the
work surface and work product. The
inflow does not mix with the clean air
within the cabinet work zone. Inflow air
travels through a return path toward the
common air plenum (blower plenum) at
the top of the cabinet.
Ambient air is taken in through a prefilter at the top of the cabinet, and
passes through the downflow ULPA filter,
entering the work zone as laminar flow.
The uniform, non-turbulent air stream
protects against cross contamination
within and throughout the work area.
ULPA-filtered air
Unfiltered / potentially contaminated air
Room air / Inflow air
Blower Efficiency
The Labculture blower system is designed
for high performance operation,
maximum energy efficiency and minimal
maintenance.
• The blower/motor can maintain airflow
velocity within 10% of original setpoint
for a 50% increase in pressure loss over
clean filters without manual speed
control adjustment.
• The permanently lubricated direct-drive
external rotor motor/blower reduces
operating costs.
• The external rotor motor design allows
for optimum cooling of the motor
during extended operations and extends
the motor bearing life.
Near the work surface, the downflow air
stream splits with a portion moving toward
the front air grille, and the remainder
moving to the rear air grille. A small
portion of the ULPA filtered downflow
enters the intake perforations at the
side capture zones (small blue arrows).
The uniform, non-turbulent air stream
protects against cross contamination
within and throughout the work area.
Near the work surface, the downflow
air stream splits with a portion moving
toward the front air grille, and the
remainder moving to the rear air grille.
A small portion of the ULPA filtered
downflow enters the intake perforations
at the side capture zones at a higher
velocity (small blue arrows).
A combination of inflow and downflow air
streams forms an air barrier that prevents
contaminated room air from entering the
work zone, and prevents work surface
emissions from escaping the work zone.
The downflow combined with the inflow
air enters the common air plenum.
All air in the common plenum is HEPA-filtered
and exhausted via a dedicated ducting
system to the external environment.
• Built-in RFI and electrical noise filters
eliminate interference with adjacent
instrumentation.
• The control panel is located on the
center of the cabinet, and angled down
for easy access by the operator.
• An integral blower hour meter tracks
operating life and aids in predictive
maintenance planning.
• Continuous monitoring of cabinet
airflow is displayed on a bright, easyto-read LCD panel.
• To prevent fan damage, a paper-catch
grille traps papers or towels that may
drop down on the drain pan, preventing
them from being pulled into the column
by fan suction.
• An integrated, temperature-compensated true airflow velocity sensor
provides an accurate airflow reading
despite room temperature fluctuation.
Sentinel Microprocessor Control,
Alarm, Monitoring System
The Esco Sentinel microprocessor-based
control system supervises operation of all
cabinet functions.
Esco Centrifugal Fan with External Rotor Motor (left) vs.
Conventional Fan with Standard Motor (right)
Esco cabinets use German made ebm-papst®
permanently lubricated, centrifugal motor/
blowers with external rotor designs.
Integrated blades norrow the profile and
eliminate the need for a motor shaft.
Motors are selected for energy efficiency,
compact design, and flat profile. The completely
integrated assembly optimizes motor cooling.
All rotating parts are unitized and balanced for
smooth, quiet, vibration-free operation.
Biological Safety Cabinet • Class II, Type B2 (Total Exhaust) Biological Safety Cabinets
• All electronic parts are contained inside
a plug-and-play module that permits
easy exchange if required.
• Microprocessor software updates are
available from Esco for download via
the Internet.
Sentinel functions are factory set to default
to ON or OFF, depending on worldwide
destination and local preferences. Default
settings can be user activated through the
touchpad data entry access.
• Automatic start-up sequence will
prepare the cabinet for normal operation
and advise when safe conditions are
established.
• An administrator controlled PIN
(Personal Identification Number) can
be set to restrict access to main menu.
• The airflow alarm can be activated
or deactivated depending on user
preference and nature of the work.
Touchpad data entry buttons permit control
settings and access to diagnostics, default
settings and hierarchical menus.
Color coded indicator lamps display green
for primary function (fan operation); blue
for secondary function (fluorescent lights
and electrical outlet); and orange for
caution (UV lamp ON).
Sentinel Microprocessor Control System, Programmable
Consult your Esco Operating Manual
or contact your Sales Representative
for information on user-preference
programming capabilities built into the
Sentinel microprocessor platform.
Cabinet Construction
Robust construction and enhanced safety
features qualify the cabinet for the most
demanding laboratory applications. The
cabinet is fully assembled and ready to install
and operate when shipped.
• The interior work area is formed from a
single piece of stainless-steel with large
radius corners to simplify cleaning.
Programmable automatic UV light
timer simplifies operation, enhances
contamination control, extends UV
lamp life and saves energy.
A graphical interface indicates cabinet performance.
Digital read-out with alpha-numeric display indicates all
input, status and alarm functions.
All functions can be user activated through touch-pad
programming access; see Operations Manual.
When programmed ON • the start-up sequence confirms status with Air Safe and local time display.
• the Personal Identification Number (PIN) access restricts unauthorized adjustments.
• an airflow alarm warns of deviations from normal velocities.
• The cabinet work zone has no welded
joints to collect contaminants or rust.
• All stainless steel work surfaces are
accessible for cleaning.
• Tray components lift and remove to
provide easy access and encourage
surface decontamination.
• A recessed central area and stainless
steel drain pan channels spills and
prevent liquids from entering the lower
filtration and blower systems.
• The drain pan is flush with the side walls
to eliminate concealed or hard-to-clean
spaces.
• There are no screws in on the front or
sides to trap contaminants or complicate
cleaning.
• Optional service fittings are offset for
easier access.
• External plumbing is concealed behind
trim panels to maintain aesthetics.
• External surfaces are coated with
Esco Isocide antimicrobial coating to
protect against surface contamination
and inhibit bacterial growth. Isocide
eliminates 99.9% of surface bacteria
within 24 hours of exposure.
Robust Cabinet Construction and Enhanced Safety Features
Service fixtures are offset for easier reach. Standard cabinets include two fixture provisions on each sidewall (one provision on each sidewall for 0.9 meter/3 ft. cabinet). Electrical outlets are mounted below service fixtures to minimize obstructions.
Helpful for certifiers, the hinged maintenance assembly opens to a fixed position on integrated, gas spring struts providing front service access.
All key components, with the exception of the blower/motor assembly, are mounted outside the air stream and away from contaminated air to permit service without decontamination. These include fluorescent lamps,
UV lamp, electrical harnesses, electronic boards and microprocessor control.
Panels enclosing potentially hazardous areas or components such as microbiological contamination or electrical
shock are color-coded red to warn service technician.
The Dynamic Chamber™ plenum minimizes physical lifting and accelerates filter change when required.
Work area containment is maintained even when removable components are lifted out for cleaning.
The one piece stainless steel work tray edges are radiused and easy to clean without crevices or joints.
The lower drain trough is a single-piece fabrication with wide open angles and a channel to direct spills to the drain.
The closed sidewall contains no perforations, air return slots or other hidden areas where contaminants can
accumulate.
Standards
Compliance
Biosafety Cabinets
Air Quality
Filtration
Electrical Safety
NSF / ANSI 49, USA
(LB2-4B2, LB2-6B2)
SFDA YY-0569, China
ISO 14644.1, Class 3, Worldwide
JIS B9920, Class 3, Japan
JIS BS5295, Class 3, Japan
US Fed Std 209E, Class 1 USA
EN-1822 (H14 & H13), Europe
IEST-RP-CC001.3, USA
IEST-RP-CC007, USA
IEST-RP-CC034.1, USA
UL-C-61010A-1, USA
CSA22.2, No.1010-192, Canada
EN 61010-1, Europe
IEC61010-1, Worldwide
Dynamic Chamber™ Plenum Design
• The front armrest is raised above the
workzone to improve comfort and
to ensure that the operator’s arms
do not block the forward airflow
perforations.
• The optional adjustable support stand
provides work surface height control.
• The frameless sash eliminates operator’s
line of sight blockage
• A generous sash opening allows for
easier access into the work zone,
provides ample room for transferring of
small equipment (See Specifications.)
High negative pressure
Negative pressure
Positive pressure
The Esco triple-wall design creates a Dynamic Chamber
plenum which surrounds contaminated areas with
negative pressure, preventing the possibility of
contamination from leaks in filter seal, gasket or
cabinet structure. The third wall conceals utilities.
Comfortable Ergonomic Design
• The 10° angled viewing window and
narrow profile front grille improves
reach into the work area.
• The instant-start 5000k fluorescent
lamp operates on an electronic ballast
to reduce heat, improve comfort and
conserve energy.
• The lamp delivers uniform lighting to
the work surface for greater comfort,
reduced glare and improved productivity;
see Technical Specifications.
Electrical Safety and Certification
All components meet or exceed applicable
safety requirements.
• Each cabinet is individually factory
tested for electrical safety.
• Documentation specific to each cabinet
serial number is maintained on file.
• UL Listed for USA and Canada.
• Certified to the US standard NSF / ANSI
49 for Class II biohazard cabinetry (refer
to Standard Compliance table at the top
of this page for the full list).
• Contact Esco or your Sales Representative
for site preparation information; see
Electrical Specifications.
The Labculture cabinet is warranted for
3 years excluding consumable parts and
accessories.
• Each cabinet is shipped with a
comprehensive user’s manual complete
with a report documenting all test
procedures.
• Additional IQ/OQ/PQ documentation is
available upon request.
• Contact your local Sales Representative
for specific warranty details or documentation requests.
Accessories and Options
Esco offers a variety of options and
accessories to meet local applications.
Contact Esco or your local Sales Representative for ordering information.
Support Stands
• Fixed height, available 711 mm (28")
or 864 mm (34"), ±38.1 mm (1.5")
-With leveling feet
-With casters
• Telescoping height stand for leveling feet, nominal range 660 mm or 960 mm (26" or 37.8")
•
Telescoping height stand for casters, nominal range 660 mm or 880 mm (26" or 34.6")
-Adjustable in 25.4 mm (1") increments
Antimicrobial Powder-Coating
Bacterial Population (10 folds)
The LB2 cabinet is engineered for comfort,
utility value and safety.
• The sliding window can be fully opened
to insert and remove larger instrumentation and equipment.
Warranty
All exterior painted surfaces are powder-coated
with Esco Isocide, an antimicrobial inhibitor to
minimize contamination. Isocide is integrated into
the coating substrate and cannot wash out or
diminish by repeated cleaning. Performance
results are available upon request. Contact Esco or your Esco Sales Representative for details.
8
6
4
2
0
4 hours
8 hours
12 hours
16 hours
20 hours
Contact Time in Hours
Biological Safety Cabinet • Class II, Type B2 (Total Exhaust) Biological Safety Cabinets
24 hours
Model LB2 Biological Safety Cabinet Technical Specifications
271 mm / 10.7" (LB2-3)
430 mm / 16.9" (LB2-4)
666 mm / 26.2" (LB2-5)
820 mm / 32.3" (LB2-6)
50 mm
(2.0")
250 mm (9.84")
250 mm (9.84")
17
1
2
8
4
715 mm (28.1")
13
3
450 mm
(17.5")
MAX.
OPENING
1568 mm (61.7")
7
9
14
15
16
180 mm
(7.1")
12
0.9 meter (3')
1.2 meter (4')
1.5 meter (5')
203 mm (8")
203 mm (8")
203 mm (8")
203 mm (8")
Working Access Height
248 mm (9.8")
248 mm (9.8")
248 mm (9.8")
248 mm (9.8")
4.UV light Retrofit Kit™ provision
6. Electrical outlet Retrofit Kit provision
(0.9 meter / 3' model - one single outlet in workzone) (1.2, 1.5 and 1.8 meter / 4',5' & 6' models - two single outlets in workzone)
5. IV-Bar Retrofit Kit provision
7. Electrical / Electronics panel
2.Blower
3.Downflow ULPA filter, angled
8. Fluorescent lamp
Accessories and Options (Cont.)
• Cradle stand, electrical hydraulic,
infinitely adjustable, with casters
- Elevates to seating or standing
work surface height
- When lowered permits movement through standard doorway
Note: Increases exterior dimensions.
Electrical Outlets and Utility Fixtures
• Electrical outlet, ground fault,
North America
• Electrical outlet, Europe / Worldwide
• Petcock (air, gas, vacuum)
-North America (American) style
-Europe / Worldwide style DIN 12898,
DIN 12919, DIN 3537
Cabinet Accessories
• Germicidal UV lamp
-Controlled by automatic UV lamp
5.5"
6
815 mm (32.1")
Testing Opening Height
1.Exhaust ULPA filter
140 mm
45 mm
1.8"
11
955 mm / 1260 mm / 1565 mm / 1870 mm
(37.6" / 49.6" / 61.6" / 73.6")
1115 mm / 1420 mm / 1725 mm / 2030 mm
(43.9" / 56.0" / 67.9" / 80.0")
*Cabinet Size
*
ACCESS
OPENING
203mm (8
")
10
5
1.8 meter (6')
9. Plugged service fixture provisions (2 on each side)
10.Stainless steel single-piece work tray
11.Stainless steel armrest
12.Drain valve Retrofit Kit provision
13.Esco Sentinel microprocessor control system
timer through Sentinel micro processor control panel
-Emission of 253.7 nanometers for
most efficient decontamination.
-Lamp is positioned away from
operator line-of-sight for safety and
proper exposure to interior surfaces.
Note: UV lamp intensity reduces over time
and its effectiveness is subject to factors such
as relative humidity in the cabinet, ambient
air temperature and microbial species in the
work zone.
• PVC armrest
-Chemically treated, improves operator comfort, easy-to-clean. 712 mm (28") standard size
• Ergonomic lab chair
-Laboratory grade construction,
meets Class 100 cleanliness; alcohol
resistant PVC materials
14.Safety glass sliding sash window
15.Single-piece stainless steel back wall and side walls
16.Removable side panel for plumbing access
17.Integral exhaust collar
-Adjustable 395-490 mm (15.6"-19.3")
• Ergonomic foot rest
-Angled, helps maintain proper
posture
-Adjustable height
-Anti-skid coating, chemical
resistant finish
• IV bar, with hooks
-Stainless steel construction
-Available for all standard Esco cabinets
• Microscope viewing device
-Mounting and viewing pouch
integrated into sash. Factory installed;
specify when ordering
General Specifications
LB2-3B_
LB2-4B_
LB2-5B_
LB2-6B_
0.9 meters ( 3')
1.2 meters ( 4')
1.5 meters ( 5')
1.8 meters ( 6')
External Dimensions
(W x D x H)
1115 x 815 x 1568 mm
40.7" x 33.3" x 61.7"
1420 x 815 x 1568 mm
52.7" x 33.3" x 61.7"
1725 x 815 x 1568 mm
64.7" x 33.3" x 61.7"
2030 x 815 x 1568 mm
80.0" x 33.3" x 61.7"
Gross Internal Dimesnsions
(W x D x H)
955 x 603 x 715 mm
37.6" x 21.3" x 28.1"
1260 x 623 x 715 mm
49.6" x 24.5" x 28.1"
1565 x 603 x 715 mm
61.6" x 21.3" x 28.1"
1870 x 603 x 715 mm
73.6" x 21.3" x 28.1"
0.44 m2 (4.7 sq.ft.)
0.59 m2 (6.4 sq.ft.)
0.74 m2 (7.9 sq.ft.)
0.89 m2 (9.6 sq.ft.)
203 mm (8")
203 mm (8")
203 mm (8")
203 mm (8")
248 mm (9.8")
248 mm (9.8")
248 mm (9.8")
248 mm (9.8")
Nominal Size
Usable Work Area
Tested Opening
Working Opening
Average Airflow
Velocity
Airflow Volume
Inflow
0.53 m/s (105 fpm)
Downflow
0.35 m/s (70 fpm)
Inflow
368 m /h (217 cfm)
489 m / h (288 cfm)
608 m3 / h (358 cfm)
725 m3 / h (427 cfm)
Downflow
702 m3/h (413 cfm)
924 m3/h (544 cfm)
1147 m3/h (675 cfm)
1369 m3/h (806 cfm)
Exhaust
1074 m /h (632 cfm)
1415 m /h (833 cfm)
1755 m /h (1033 cfm)
2097 m3/h (1234 cfm)
+50%
820 Pa / 3.29 in H20
690 Pa / 2.77 in H20
640 Pa / 2.57 in H20
620 Pa / 2.49 in H20
+100%
1060 Pa / 4.26 in H20
880 Pa / 3.53 in H20
810 Pa / 3.25 in H20
770 Pa / 3.09 in H20
Pressure Capacity
Requirements for
Exhaust Ducting*
3
3
3
3
Supply ULPA Filter Typical Efficiency
>99.999% for particle size between 0.1 to 0.3 microns.
Exhaust HEPA Filter Typical Efficiency
>99.99% at 0.3 microns.
Sound Emission
NSF / ANSI 49
<62.5 dBA
<62.5 dBA
<64 dBA
<64.5 dBA
EN 12469
<59.5 dBA
<59.5 dBA
<61 dBA
<61.5 dBA
> 1335 Lux
(> 124 foot-candles)
> 1440 Lux
(> 134 foot-candles)
> 1200 Lux
(> 112 foot-candles)
> 1230 Lux
(> 114 foot-candles)
Fluorescent Lamp Intensity At Zero Ambient
3
Cabinet
Construction
Electrical **
LB2-4B2, LB2-6B2
1.5 mm (0.06") 16 gauge electro galvanized steel with Isocide white oven-baked epoxy polyester powder-coating
All other variants
1.2 mm (0.05") 18 gauge electro galvanized steel with Isocide white oven-baked epoxy polyester powder-coating
220-240V, AC, 50Hz, 1Ø
LB2-3B1
LB2-4B1
LB2-5B1
LB2-6B1
110-130V, AC, 60Hz, 1Ø
LB2-3B2
LB2-4B2
LB2-5B2
LB2-6B2
LB2-3B3
LB2-4B3
LB2-5B3
LB2-6B3
Net Weight ***
220-240V, AC, 60Hz, 1Ø
288 kg / 634 lbs
335 kg / 739 lbs
386 kg / 851 lbs
438 kg / 906 lbs
Shipping Weight ***
336 kg / 741 lbs
391 kg / 862 lbs
450 kg / 991 lbs
508 kg / 1120 lbs
1230 x 940 x 1900 mm
49.8" x 35.4" x 73.4"
1530 x 940 x 1900 mm
59.1" x 35.4" x 73.4"
1910 x 940 x 1900 mm
74.8" x 35.4" x 74.8"
2150 x 940 x 1980 mm
82.7" x 35.4" x 74.8"
2.20 m3 (71.3 cu.ft.)
2.73 m3 (89.2 cu.ft.)
3.41 m3 (114.7 cu.ft.)
4.00 m3 (126.8 cu.ft.)
Shipping Dimensions,
Maximum (W x D x H) ***
Shipping Volume, Maximum ***
*
These figures are the required exhaust fan pressure capacity left at the cabinet's duct connection, plus 50% and 100% extra safety margin, but not including the
presure capacity required to overcome the pressure drop from bends, elbows, valves, etc in the duct work. These required pressure capacity has been claculated to
overcome the increased exhaust HEPA filter pressure drop by +50% and +100% due to particle loading. The minimum recommended pressure capcity is +50%,
but to prolong the "life" of exhaust filter, +100% is a safer choice.
** Additional voltages may be available; contact Esco for ordering information.
*** Cabinet only, excludes optional stand.
Comprehensive Performance Testing At Esco
Every Labculture LB2 model manufactured by Esco is
individually tested, documented by serial number and
validated with the following test methods.
Inflow / downflow velocity
PAO aerosol challenge for filter integrity
Light, noise and vibration
Airflow pattern visualization
Electrical safety to IEC61010-1
KI-Discus containment
Biological Safety Cabinet • Class II, Type B2 (Total Exhaust) Biological Safety Cabinets
Additional microbiological testing is performed on statistical sampling basis.
Microbiological Testing
Cross Contamination Test
Esco performs testing in accordance
with more than 10 of the world’s most
recognized standards for local, regional
and international criteria. Testing in our
microbiology laboratory is conducted
according to NSF / ANSI 49, EN 12469, and
JIS K3800. An NSF-accredited biohazard
cabinet field certifier is available in-house
full-time to supervise all tests.
The test objective is to evaluate cabinet
protection from cross contamination of
samples placed simultaneously inside the
workzone.
Harmless Bacillus atrophaeus (formerly
Bacillus Subtilis) bacteria is used to
challenge the cabinet, then incubated for
48 hours and the Colony Forming Units
(CFU) are counted to determine the testing
results. Increased microbiological challenge
tests with objects inside the cabinet work
zone, Bunsen burner, external airflow
disturbance, and Human-As-Mannequin
test adapted from Fume Hood development
were performed to simulate real-world
conditions.
Personnel Protection Test
The test objective is to evaluate the safety
of the cabinet for the personnel operating
on potentially hazardous samples in the
cabinet workzone.
• A nebulizer containing 55 mL of 5 to 8
x 108 spores/mL B.atrophaeus spores is
placed inside the workzone, 10 cm (4
inches) behind the front opening sash.
• A nebulizer containing 55 mL of spores
(5 to 8 x 104/mL) is placed against one
of the workzone sidewalls.
• Target agar plates are placed 36 cm
(14”) away from the same side wall as
the nebulizer.
• Acceptance: The number of Bacillus
atrophaeus CFU recovered on agar
plates shall not exceed 2 CFU per test.
HPV Test Compliant:
Safer Hydrogen Peroxide
Decontamination Compatibility
Esco biological safety cabinets are Hydrogen
Peroxide Vapor (HPV) compliant and
decontaminatable cabinets approved by
BIOQUELL for this patented process. HPV
is a safer and more efficient alternative
to conventional decontamination using
formaldehyde (CH20):
• HPV is non-carcinogenic and odorless,
while formaldehyde is carcinogenic,
toxic and has pungent smell.
• HPV biological efficacy is independent
of environmental variables, whereas
formaldehyde efficacy is dependent on
such variables.
• Target slit air samplers and impingers
are placed outside the workzone to
capture B.atrophaeus spores that may
escape, then the sample is incubated
• Acceptance: The number of B.
atrophaeus CFU recovered from the
agar plates shall not exceed 10 CFU
per test.
0.65
m/s
• Target agar plates are placed throughout
the entire work surface.
• Acceptance: The number of Bacillus
atrophaeus CFU recovered from the
agar plates shall not exceed 5 CFU
per test.
0.60
• HPV is more effective and rapid against
biological organisms compared to
formaldehyde.
• HPV requires approximately 4-7
hours for set-up, decontamination,
and tear-down, compared to a total
of 12-15 hours needed to complete
a formaldehyde decontamination
process.
• HPV decontamination effectiveness
is independent of temperature and
humidity. Formaldehyde requires
temperature above 20°C and relative
humidity above 65%
• For information on the BIOQUELL HPV
methodology contact Esco or your Sales
Representative.
High Inflow
High Downflow
(0.61, 0.43)
High Inflow
Low Downflow
(0.58, 0.30)
The graph illustrates the boundaries of
the performance envelope, as well as the
nominal performance point at which tests
are conducted.
0.50
0.45
Esco cabinets are designed to operate within a
performance envelope to maintain protection
for personnel, product and the environment.
Airflow parameters used to frame the
performance envelope include both inflow
velocity and downflow velocity.
Nominal Inflow
Nominal Downflow
(0.53, 0.35)
0.55
Inflow Velocity
• A nebulizer containing 55 mL of 5 to 8
x 106 spores/mL B.atrophaeus is placed
at 10 cm (4 inches) in front of sash
window.
• HPV has a better penetration capacity,
resulting in a full decontamination of
the cabinet. The formaldehyde method
is known to result in incomplete
decontamination,
The Performance Envelope Concept
Product Protection Test
The test objective is to determine cabinet
protection to the product/samples inside
the cabinet workzone from environmental
contaminants.
• If there is a gap on the cabinet sealing,
escaping HPV to the lab will decompose
to become oxygen and water. Escaping
formaldehyde, however, is harmful
to people in the lab. Therefore HPV
decontamination can be performed
while people still working inside the lab,
while formaldehyde decontamination
must be performed with no one present
in the lab. The HPV method improves
safety, productivity, and reduces the
time to seal the cabinet.
Low Inflow
High Downflow
(0.48, 0.40)
Low Inflow
Low Downflow
(0.48, 0.30)
0.40
The range between high and low Inflow,
and high and low downflow, together
with the fluid dynamics achieved through
sophisticated cabinet design, proportionally
size capture slots, and uniform laminar
airflow, combine to deliver a complete
containment and safety solution expected of a professional biological safety cabinet.
For details on the Esco performance envelope
0.25
0.30
0.35
0.40
Downflow velocity
0.45
0.50 contact Esco or your Esco Sales Representative.
m/s
KI Discus Containment
Test According to EN 12469
(Potassium Iodide)
Esco is currently one of the few companies
in the world equipped to perform the KI
Discus test for our customers. The KI Discus
test is defined in the European Standard
for microbiological safety cabinets, EN
12469, as a test method for validating the
operator/personnel protection capabilities
of the cabinet.
• The KI Discus test shows excellent
correlation with the microbiological
test method for operator protection,
and is useful for validating the actual
containment performance of the cabinet
on-site.
• The KI-Discus takes only 45 minutes as
opposed to 2 days for microbiological
testing.
• Thus, each Esco Labculture LB2 model
is factory tested using the KI Discus
method for operator safety.
Purchase Specifications
10
LB2 Series Class II, Type B2 (Total Exhaust)
Biological Safety Cabinet
General Performance and Certifications
1. The biological safety cabinet shall comply with
one or more of the following international
standards, and the manufacturer shall provide a
certified copy of containment and performance
tests equivalent to or greater than specified
in the following independent international
standards for biological safety, electrical and
other functional characteristics: Class II, Type
B2 per NSF / ANSI 49 (USA).
2. The cabinet shall protect (a) the operator
and laboratory environment from particulates
generated within the work zone; (b) the product
and process within the work zone from airborne
contamination from ambient air; (c) and the
product and process within the work zone from
cross contamination.
3. Before shipment each cabinet shall be individually
tested by the KI-Discus test (European Standard EN
12469) to validate operator/personnel protection.
The retention efficiency for the front aperture shall
be not less than 99.999%. Microbiological testing
for cabinet performance shall be performed on a
statistical sampling basis.
4. Each cabinet shall be listed by Underwriters’
Laboratories (UL, CUL) or CE for electrical safety.
5. Original documentation specific to each cabinet
serial number shall be provided with the cabinet
and maintained in the manufacturers’ records.
Test data verifying all performance criteria shall
be available upon request to include: (a) inflow
velocity through direct inflow measurement
method; (b) downflow velocity and uniformity;
(c) filter leak scan with aerosol challenge for
both filters; (d) light, noise, vibration; (e) and
electrical safety.
Filtration System
26.Magnetic, not mechanical, proximity sensors shall
work in conjunction with the control system to
indicate proper sash position for containment.
6. The cabinet shall have one supply downflow
filter and one exhaust filter. Supply filter shall
be ULPA-type per IEST-RP-CC001.3 and meet
EN1822 (H14) requirements. Exhaust filter shall
be HEPA-type per IEST-RP-CC001.3 and meet EN
1822 (H13) requirements.
27.Fluorescent lamps shall be mounted behind the
control panel module out of the work zone.
Electronic ballasts shall be used to eliminate
flicker, extend lamp life and reduce heat output.
7. The filters shall be within an aluminum frame
with mini-pleat design without aluminum
separators; no wood or fiberboard shall be used
in the filter assembly.
28.The UV lamp, if installed, shall be mounted
behind the control panel and away from the
user’s line of site for protection. (Note: Does not
apply to 1.8 meter/ 6 ft. model.)
8. Typical ULPA filter efficiency shall be >99.9997% at
MPPS and 99.999% for 0.1 to 0.3 microns. Typical
HEPA Filter shall be >99.99% at 0.3 microns.
9. An integral filter guard shall be affixed to
prevent damage to the filter media.
29.The UV lamp shall operate via an automatic
timer with automatic shut-off managed by the
microprocessor controller and shall be interlocked
with the blower/motor and fluorescent lights for
safety.
10. The filters shall be (a) individually scan tested by
the manufacturer, (b) individually scan tested after
assembly, and (b) easily accessible for scan testing
in situ by means of a dedicated upstream sampling
port accessible from within the cabinet.
30.The cabinet shall be designed with a 10° angled
front to optimize user comfort, reduce glare and
maximize reach into the work area.
31.The front grille shall be raised to prevent airflow
blockage and improve comfort.
11.The supply filter shall be angled and oriented
to the 10° cabinet front angle to maximize
downflow uniformity over the work surface.
32.Penetrations for petcocks and service fittings
shall be provided; penetrations shall be offset to
improve user access.
12.A removable, perforated metal diffuser shall
be installed below the supply filter to optimize
airflow uniformity and to protect from damage.
33.The cabinet shall accommodate an optional
mounting stand for fixed-height.
Blower System
13.The cabinet shall have a direct drive, permanently
lubricated centrifugal blower/motor dynamically
balanced in two planes compliant to ISO 2710
for low noise, low vibration and long filter life.
14.The blower/motor shall have an external rotor
design and include an automatic thermal cut-out
to disable the motor in case of overheating.
15.The blower/motor shall have an automatic speed
control to compensate for filter loading.
16.The blower/motor system shall be enclosed
within a dynamic chamber shaped steel plenum
and integrated with the removable supply filter
assembly to simplify filter changing.
17.The cabinet shall be equipped with an integral
exhaust collar for connection to the laboratory
ducting system.
Cabinet Design, Construction, Cleaning
18.The cabinet shall be of triple wall design whereby
all positive pressure plenums capable of handling
contaminated air shall be surrounded by negative
pressure. No positive pressure areas shall be
accessible external to the cabinet. The third wall
shall conceal utilities.
19.The cabinet shall maintain containment
performance even when removable work area
components are removed for cleaning.
20.The work tray shall be one-piece, removable,
stainless steel with radius corners without crevices
or joints.
21.The cabinet shall have a stainless steel, onepiece fabricated drain trough with open angles
to channel spills to a common drain.
22.The closed sidewall shall be sealed without
perforations, return air slots or concealed areas
which can contain contaminants.
23.The cabinet shall be free of sharp edges, nonfunctional protrusions, bolts, screws or hardware,
and all metal edges shall be deburred.
Ergonomics and Convenience
24.The front sash shall be frameless to maximize
visibility, and accessible for cleaning front and
back. Sash glass shall be safety glass.
25.The sash counterbalance shall be suspended on
two high-strength cables, and the sash shall lock
into position in the event one cable becomes
detached.
Biological Safety Cabinet • Class II, Type B2 (Total Exhaust) Biological Safety Cabinets
Control and Alarm System
34.All cabinet functions shall be managed by a
programmable microprocessor control system
capable of software updates via Internet
downloads.
35.The microprocessor controller shall be mounted
on the main control panel facing down toward
the user.
36.The controller shall include soft-touch keypad
controls and backlit LCD displays to permit
operation of the blower/motor, light, UV lamp,
electrical outlet(s) and menu.
37.The controller shall be user programmable
in situ to enable or disable functions such as
PIN (personal identification number) access
restriction, cabinet start-up protocol, airflow
alarm and other microprocessor controlled
operations outlined in the user manual.
38.When programmed ON, the start-up protocol
shall perform an automatic pre-purge and postpurge cycle to ensure proper cabinet operation.
39.The controller shall include a blower/motor hours
meter to display aggregate motor running time
to assist in predictive maintenance.
40.Audible and visual alarms shall be provided for
unsafe conditions such as improper airflow or
sash position.
41.Airflow shall be monitored by a temperature
compensating, thermistor-based, true air velocity
sensor mounted in the cabinet.
42.The airflow display and alarm system shall be
individually calibrated before shipment.
43. The main control panel shall exhibit continuous
display of air velocity and a 24-hour clock display.
Certification, Service and Decontamination
44. The cabinet shall be approved for both hydrogen
peroxide vapor (HPV) and formaldehyde
decontamination protocol.
45. All panels leading to potentially contaminated and/
or hazardous areas shall be color coded red.
46. All components with the exception of blower/
motor and ULPA filters shall be located outside
of contaminated air spaces to facilitate servicing
without the need to decontaminate the cabinet.
47. All exterior surfaces shall be painted with a
permanent antimicrobial inhibitor coating to
minimize contamination.
ESCO Airstream® Class II Type B2 Biosafety Cabinets
Class II Type B2 biohazard safety cabinets provide product,
operator and environmental protection and are suitable
for general microbiological work with agents assigned to
biological safety levels I, II or III. In a Class II Type B2
cabinet, all inflow and downflow air is exhausted after
HEPA/ULPA filtration to the external environment without
recirculation within the cabinet.
These cabinets are supplied with an integral exhaust
collar that allows the cabinet exhaust to be ducted to the
external environment via a dedicated exhaust system.
As they incorporate a non-recirculating total exhaust
design, Class II Type B2 cabinets are suitable for work with
toxic chemicals when these are present in trace amounts.
Main Filtration Agents
 ISO Class 3 air cleanliness within
workzone as per ISO 14644.1 (equivalent
to Class 1 as per the US Federal Standard
209E, 100 times cleaner than the usual
Class 100 classification on cabinets offered
by the competition).
 Minipleat separatorless H13 HEPA /
U15 ULPA filter technology, operating
at the typical efficiency of 99.999% at
MPPS, 0.3 and 0.12 microns provides
better operator, product and environmental
protection than conventional HEPA filters.
CABINET CONTROL SYSTEM
 Esco Sentinel™ Microprocessor
Cabinet Control System allows the user
to easily access all cabinet functions. The
control system monitors and displays
cabinet airflow constantly on the control
LCD screen, prompting the user with
audible and visual alarms in case of any
unsafe condition.
 True airflow velocity sensing technology, with temperature compensation for
improved sensor accuracy.
 Audible and visual alarms for low airflow,
unsafe sash positions.
 Automatic warm-up cycle is enabled
upon turning on the cabinet, ensuring all
contaminants are purged from the cabinet
workzone before the operator can use the
cabinet. All menu functions are inactive
during this period. Automatic post-purge
cycle can be configured at shutdown,
ensuring all residue contaminants are
purged out of the workzone before
deactivation.
 Fail-safe system ensures that in case
of exhaust failure, the cabinet’s main fan
automatically shuts down to ensure safety
to the user and the environment.
 An admin PIN can be set to restrict
unauthorized access to all menu functions.
ERGONOMIC FEATURES
 Attractive stainless steel internal
side walls and back wall are made
from a single sheet of stainless steel,
which eliminates welded joints where
contaminants may accummulate. The
single-piece inner liner enhances the
cleanability of the workzone and is nonglaring for operator comfort.
 Additional UV-interlock ensures UV lamp
feature is deactivated when the sash is not
fully closed.
 Built-in 5000k fluorescent lighting
offers excellent illumination throughout
the workzone. Electronically-ballasted
lighting system is instant-start, nonflickering and energy-efficient.
 Sloped front design and frameless
front sliding sash allow for maximum
visibility into the workzone.
 Cabinet armrest is raised above the
workzone to ensure that the operator’s arms
do not block the front inflow perforations.
CABINET BODY CONSTRUCTION
 All components designed for maximum
chemical resistance for long service life
and durability. All cabinet components are
cleanroom compatible.
 Industrial-grade main body constructed
of electrogalvanised steel: with an abrasionresistant white oven-baked powder-coated
finish. Attractive single-piece stainless steel
work surface is easy to remove.
 Inherently safe design maintains
containment for protection even with work
tray removed, thus ensuring safety for the
operator during cleaning.
 Permanently lubricated direct drive
centrifugal blower(s); energy efficient
external rotor motor type design reduces
operating costs; extremely low noise and
vibration levels (less than 64dBA at working
position) due to proprietary construction
and mounting technology.
 Built-in solid state variable speed
controller(s) (infinitely adjustable from zero
to the maximum setting) with built-in RFI
and noise filters is superior to conventional
“step” controllers.
 Superior electrical safety for the
operator; all electrical components are
UL listed or UL recognized; all cabinets are
factory tested for electrical safety after
production at manufacturing site.
 Designed to meet the general safety
requirements of the IEC 61010-1 / EN 610101 / UL 61010A-1 / CSA C22.2 No. 1010.1-92.
 Cabinet is shipped fully-assembled;
simply plug the unit into a power source
for operation - no local installation is
required; unit is supplied with a 3-pin plug;
10 international plug types are available
(specify order code when ordering).
Esco Catalogue Revision No. AB2.v2.03.2005
Specifications subject to change without notice.
© 2005 Esco Micro Pte Ltd.
Technical Specifications and Engineering Diagram
100 mm
Ø12.0”
3.9”
Ø305 mm
1
9
3
ESCO
10
4
6
5
7
11
173 mm
6.8”
12
8
670 mm / 26.4”
Air Flow 0.3 m/s
1460 mm / 57.5”
1360 mm / 53.5”
2
13
1270 / 1880 mm
50.0” / 74.0”
710 mm / 28.0”
1340 / 1950 mm
52.8” / 76.8”
14
1. Exhaust HEPA Filter 2. Blower 3. Downflow ULPA Filter
4. Fluorescent Lamp 5. IV Bar Retrofit Kit™ Provision
6. UV Light Retrofit Kit™ Provision
7. Service Fixture Retrofit Kit™ Provisions (2 on each sidewall)
8. Internal Single-Piece Stainless Steel Work Zone
9. Electrical/Electronics Module
10. Esco Sentinel™ Microprocessor Control
11. Tempered Glass Sash Window
12. Electrical Outlet Retrofit Kit™ Provisions (2 no’s)
13. Single-Piece Stainless Steel Work Tray 14. Pre-Filter
General Specifications
External Dimensions (L x W x H)
Internal Work Zone (L x W x H)
AB2-4SX*
AB2-6SX*
1340 x 710 x 1460 mm / 52.8” x 28.0” x 57.5”
1950 x 710 x 1460 mm / 76.8” x 28.0” x 57.5”
1270 x 550 x 670 mm / 50.0” x 21.7” x 26.4”
1880 x 550 x 670 mm / 74.0” x 21.7” x 26.4”
Designed to meet and exceed the requirements of: EN 12469:2000 and ANSI / NSF49 Standards
Air cleanliness: ISO 14644.1 Class 3, IEST-G-CC1001, IEST-G-CC1002 and other equivalent requirements
Standards Compliance
Filter performance: IEST-RP-CC034.1, IEST-RP-CC007.1, IEST-RP-CC001.3 and EN1822
Electrical safety: IEC 61010-1 / EN 61010-1 / UL 61010A-1 / CSA C22.2 No. 1010.1-92
Average Airflow
Inflow
Initial setpoint: 0.53 m/s or 105 fpm
Velocities
Downflow
Initial setpoint: 0.31 m/s or 61 fpm (uniformity is +/-20%)
Inflow
419 cmh / 246 cfm
621 cmh / 365 cfm
Airflow Volumes
Downflow
897 cmh / 528 cfm
1327 cmh / 781 cfm
At Initial Airflow
1166 cmh / 686 cfm
1714 cmh / 1008 cfm
Velocity Setpoints Total Exhaust
Audible/visual alarm will activate when exhaust airflow volume drops below 12% of the initial volume.
Pressure Drop Requirements for
440 Pa / 1.77” WG
365 Pa / 1.47” WG
Exhaust Ducting
ISO 14644.1 Class 3, US Federal Standard 209E Class 1 / M1.5, AS 1386 Class 1.5, JIS B9920 Class 3, BS5295 Class C,
Cleanliness Within Working Area
Class M10,000 as per KS 27030.1 and equivalent classes of VDI 2083 and AFNOR X44101
ULPA filter with integral metal guards and filter frame gaskets;
Downflow Filter Type
Typical efficiency 99.999% at 0.3µm, 0.12µm and MPPS; fully compliant with EN 1822 and IEST-RP-CC001.3 requirements
HEPA filter with integral metal guards and filter frame gaskets;
Exhaust Filter Type
Typical efficiency 99.99% at 0.3µm; fully compliant with EN 1822 and IEST-RP-CC001.3 requirements
*Choose from the following options and specify option number when ordering, (e.g. AB2-4S1 for 220-240VAC 50HZ)
Power Supply Options
1. 220-240VAC 50HZ, 1 phase 2. 110-130VAC 60HZ, 1 phase 3. 220-240VAC 60HZ, 1 phase
4. 110-130VAC 50HZ, 1 phase 5. 100-110VAC 50HZ / 60HZ
ESCO
®
Esco Biotechnology Equipment Division
PT Esco Bintan Indonesia
Your local distributor:
Esco Biotech is a highly focused manufacturer of laminar flow, biohazard
safety and other HEPA-filtered cabinets for the laboratory with a history
of quality cabinets since 1978. We are predominantly oriented towards the
international marketplace, with sales in more than 70 countries and 95% of
turnover exported. Our products have been independently tested to standards such as AS1807.5 and EN12469. Products are manufactured under
an ISO 9001 registered quality system.
21 Changi South Street 1 · Singapore 486 777 · Tel: +65 6542 0833 · Fax: +65 6542 6920 · Email: [email protected] · biotech.escoglobal.com
Global Offices: Philadelphia, USA ~ Leiden, Netherlands ~ Santiago, Chile ~ Shanghai, China ~ Kuala Lumpur, Malaysia ~ Mumbai, India ~ Dubai, U.A.E.
ESCO Airstream® Class III Biosafety Cabinet
Esco Airstream® Class III biohazard safety
cabinets offer the highest level of product,
operator and environmental protection from
infectious/biohazardous aerosols and are suitable
for microbiological work with agents assigned to
biological safety levels 1, 2, 3, or 4. Designed for an
absolute level of containment, they are frequently
used for work involving the deadliest biohazards,
bacteria, viruses and microorganisms.
Manufactured to meet and exceed the latest Class
III biohazard safety cabinet requirements of the
EN 12469:2000.
MAIN FILTRATION AGENTS
ISO Class 3 air cleanliness within work
zone as per ISO 14644.1 (equivalent to
Class 1 as per US Federal Standard 209E,
100 times “cleaner” than the usual Class
100 classification on cabinets offered by
the competition). All materials used in our
products are cleanroom compatible.
Mini-pleat separatorless ULPA filter
technology reduces energy consumption and
delivers increased laminar airflow uniformity
for better product and cross contamination
protection. ULPA filters are double scantested, at the time of manufacturing, and
after installation.
Exclusive vertical laminar flow design
ensures that air within the chamber is
decontaminated in a consistent fashion,
thus preventing the accumulation of
particulate contamination in “dead air
corners”. Product protection is guaranteed
by the ULPA-filtered vertical laminar air flow
within the cabinet workzone.
Exhaust air is double-filtered through
high-quality ULPA filters with a rated
efficiency of 99.999% at 0.12 microns,
thus ensuring complete product, operator
and environmental protection from airborne
biological hazards; a built-in pre-filter also
extends main filter life.
CONTROL FEATURES
Microprocessor-based Esco Sentinel™
control system provides visual / audible
alarms for airflow. Easy-to-clean membrane
touch control panel is supplied with an LCD
display.
Magnehelic pressure gauge* is mounted
in the backwall of the workzone for monitoring
pressure in the workzone during usage.
*Registered trademark of Dwyer
Instruments, Inc
CONSTRUCTION FEATURES
State-of-the-art Neoprene™ synthetic
arm-length gloves; flame and abrasionresistant, cleaner, and more resistant to
chemicals and flames than regular latex
gloves; each glove is individually tested
for airholes. Glove ports are designed to
make glove-changing easy and most
importantly, absolutely safe. Gloves
available in sizes 6 and 7.
Integrated pass-through with
interlocking doors at side of main chamber
allows materials to be transferred into the
cabinet without the risk of environmental
contamination.
A sloped cabinet front ensures an
ergonomic working posture for increased
operator productivity and reduces glare
from the glass surface.
Durable and easy to clean stainless
steel work surface will never rust or cause
contamination.
Built-in warm white, electronically
ballasted lighting is comfortable to the eyes
and offers excellent illumination throughout
the work zone in order to reduce operator
fatigue. Light tubes are mounted out of the
air stream for better airflow uniformity.
UV-resistant tempered glass window
with built-in stainless steel glove ports
(for the number of gloves ports, refer to
engineering diagrams provided) is designed
to be 100% leak-free.
Industrial-grade support frame constructed
of electro-galvanised steel with an abrasionresistant oven-baked powder-coated
finish.
Cabinet airflow is regulated by double fans
(refer to diagrams on page 3), thus ensuring
a fail-safe system that guarantees absolute
safety.
Permanently lubricated direct drive
centrifugal blower(s); energy efficient
external rotor motor type design reduces
operating costs.
Built-in solid state variable speed controller
(infinitely adjustable from zero to the
maximum setting) with built-in RFI and
noise filters is superior to conventional
“step” controllers.
Complete welded construction ensures an
air-tight cabinet carcass for absolute
containment and operator/environmental
safety.
Exclusive Dynamic Chamber™ design
surrounds all contaminated air plenums with
negative pressure plenums for additional
safety. Unique airflow technology maintains
cabinet negative pressure at -274Pa
/ 1.1inWater within the chamber under
all circumstances for maximum safety and
containment.
Designed to meet the safety
requirements of IEC 61010-1 / EN
61010-1 / UL 3101-1 / CSA C22.2 No.
1010.1-92. Components are UL listed
/ recognised.
Extended warranty period of 3
years excluding consumable parts and
accessories.
General Features Cont. / Class III Cabinet Airflow Profile
INDIVIDUAL PERFORMANCE TESTING
Your safety means the world to us: the following tests are
performed individually at our in-house laboratory on every cabinet
produced:
a. Airflow velocities
b. Cabinet leak test
c. Aerosol challenge test for filter integrity
d. Light intensity / noise / vibration levels
e. Airflow pattern visualisation test
f. Electrical safety tests
A detailed report is included with every cabinet detailing the tests
performed and results obtained.
Extensive testing is continuously performed at our research and
development laboratories to improve cabinet containment and
performance.
Recommended re-certification is 12 months from initial date of
operation; in addition, we recommend that where possible, onsite testing after delivery and installation should be conducted in
accordance with the following standard: EN12469:2000 (Section
6 Table 5).
Exhaust Air is
ULPA-filtered
Esco Airstream® Class III Biohazard Safety Cabinet: Airflow Profile
• Air taken in from above the cabinet
passes through a pre-filter and an ULPA
filter. Moving into the main chamber of
the cabinet in a vertical air stream, it
provides product protection from airborne
particulate matter and cross contamination.
This vertical flow air stream is also known
as the downflow.
Intake Air

Pre-filter
Removes
Larger
Particles
• Close to the work surface, the downflow
air stream “splits” with a portion entering
grilles towards the front or back of the
cabinet, providing a uniform downflow over
the entire working area of the chamber.

• Esco’s unique airflow design ensures that air
within the cabinet is replaced consistently,
eliminating excessive air turbulence and the
accumulation of particulate contamination
in “dead air corners”.
Positive Pressure Plenum
Vertical Airflow

• Air is moved within the cabinet below
the work table, where is passes through
two ULPA filters; one mounted below
the work table, and the other above the
main chamber. This provides a fail-safe
mechanism in case any one of the ULPA
filters fails to maintain containment.
• Esco’s unique blower system maintains
negative pressure within the main work
chamber under all circumstances. Unlike
the competition, our Class III biohazard
safety cabinets do not utilize supply
blowers, which, when improperly balanced,
may result in the working area being
positively pressurized.
Downflow / Supply ULPA Filter
First Exhaust ULPA Filter
Second Exhaust ULPA Filter
• All exhaust air is ULPA-filtered and
exhausted directly back to the laboratory
without re-circulation. Optionally, an
exhaust collar can be fitted to allow air to
be ducted via a dedicated ducting system
to the external environment.
• Air velocity through an open glove port will
be a minimum of 0.7m/s (140fpm) when a
single port is open.
Class III Biohazard Safety Cabinets are
commonly specified for use with the
world’s deadliest microbes. Drawing on the
resources and expertise of our cleanroom
engineering division, Esco is able to
provide a complete turn-key service for
the construction of critical environments to
complement applications in which Class III
cabinets may be used.
Although a Class III safety cabinet provides
an unparalleled degree of operator and
environmental protection, safety can
still be increased by the construction of
negative pressure laboratories and the
use of isolation suits. When a Class III
safety cabinet is operated within a negative
pressure laboratory, any failure in the
Class III cabinet will still be isolated within
the laboratory. Assuming the cabinet
fails, contamination from the cabinet will
“spill-over” into the laboratory but will not
contaminate the general environment,
since all air within the laboratory is filtered
before being exhausted to the external
environment.
Engineering Diagrams for Available Sizes
GLOVE SIZES
To find out your glove size, measure (in inches) around your hand with a tape measure at the
place indicated on the diagram. This measurement, in inches, is the closest to your glove size,
i.e. 8” is equal to a glove size of 8.
You should use your dominant hand, the right if you are right-handed, and the left if you are lefthanded.
AC3-4 (Standard Model)
External Width: 1.34m / 4ft
AC3-5
External Width: 1.645m / 5ft
15
15
1
12
1
12
Air Flow 0.3 m/s
ESCO
13
Air Flow 0.3 m/s
ESCO
13
2
14
2
15
400 mm
400 mm
15.7”
15.7”
400 mm
15.7”
450 mm
17.7”
1340 mm / 52.8”
330 mm
400 mm
13.0”
15.7”
1645 mm / 64.8”
1665 mm / 65.6”
1970 mm / 77.6”
AC3-6
External Width: 1.95m / 6ft
AC3 Side Diagram for All Sizes
15
9.8”
250 mm
250 mm
9.8”
3
10
11
4
13
290 mm / 11.4”
2
7
14
650 mm / 25.6”
ESCO
2250 mm / 88.6”
5
6
Air Flow 0.3 m/s
340 mm / 13.4”
1
12
400 mm
15.7”
400 mm / 15.7”
8
900 mm / 35.4”
450 mm / 17.7”
450 mm / 17.7”
9
1950 mm / 76.8”
850 mm / 33.5”
2275 mm / 89.6”
Engineering Details
1. Esco Sentinel™ Microprocessor Control 2. Pass Box 3. Pre-filter 4. Electrical / Electronics Panel
5. ULPA Filter (Downflow) 6. Fluorescent Light 7. Glove Ports (Gloves available in sizes 7,8 & 9)
8. First Exhaust ULPA Filter 9. First Exhaust Blower 10. Second Exhaust ULPA Filter 11. Second Exhaust Blower
12. Optional UV Lamps 13. Pressure Gauge
14. Work Zone Back Wall: Optional Electrical Outlet Retrofit Kit™ Provisions: 2 outlets in work zone one on each side)
15. Optional Exhaust Collar
Esco Catalogue Revision No: AC3.v3.6.2005
Specifications subject to change without notice.
©2005 Esco Micro Pte. Ltd.
Technical Specifications
Custom-made Cabinets
Optional Retrofit Kits™ and Accessories
Esco Airstream® Class
III biosafety cabinets are
available in full stainless
steel on a customized
basis, suitable for the
pharmaceutical industry.
The following optional Retrofit Kits™ are available from Esco for
Airstream Class III biohazard safety cabinets. Esco’s Retrofit
Kit™ system allows the user to field-upgrade their cabinets with
optional accessories post-purchase. NOTE: Service fixtures are
not allowed in Class III biosafety cabinets, as Class III biosafety
cabinet construction is required to be airtight.
Contact Esco for more
information.
-Germicidal UV lamp Retrofit Kit™
Left: Custom-made 4ft
fully stainless steel Esco
Airstream® Class III cabinet
(AC3-4SX)
-Standard electrical socket outlet Retrofit Kits™ (total 6A
rating for all outlets in the work zone). GFCI
(ground fault circuit interrupter) electrical
socket outlets (115VAC units only).
-Hard (air-tight) duct exhaust collar
-Formalin vaporiser (see image). For
biosafety cabinet decontamination.
General Specifications
External Dimensions
(Width x Depth x Height)
Internal Work Zone
(Width x Depth x Height)
Number of Glove Ports
Glove Type & Sizes Available
Air Volume (At Initial Velocity)
Cabinet negative pressure
Standard
Size
AC3-4BX
AC3-5BX
AC3-6BX
1340 x 850 x 2250 mm
1645 x 850 x 2250 mm
1950 x 850 x 2250 mm
52.8” x 33.5” x 88.6”
64.8” x 33.5” x 88.6”
76.8” x 33.5” x 88.6”
1240 x 650 x 650 mm
1545 x 650 x 650 mm
1850 x 650 x 650 mm
48.8” x 25.6” x 25.6”
60.8” x 25.6” x 25.6”
72.8” x 25.6” x 25.6”
2 ports (refer to drawing on page 3)
4 ports (refer to drawing on page 3)
4 ports (refer to drawing on page 3)
Neoprene® polychloroprene synthetic rubber gloves: Available in sizes 7, 8 (standard size) and 9.
603 cmh / 355 cfm
756 cmh / 445 cfm
902 cmh / 531 cfm
274Pa / 1.1inH2O (exceeds EN12469:2000 requirement of 199.3Pa / 0.8inH2O and NSF49 requirement of 124.5Pa / 0.5inH2O
Individually performance tested and certified at factory under controlled conditions for:
General requirements: IEST-RP-CC002.2 and AS1386.5
Standards Compliance
Air cleanliness: ISO 14644.1 Class 3, IEST-G-CC1001, IEST-G-CC1002 and other equivalent air cleanliness requirements
Filter performance: IEST-RP-CC034.1, IEST-RP-CC007.1, IEST-RP-CC001.3 and EN1822
Electrical safety: IEC 61010-1 / EN 61010-1 / UL 61010A-1 / CSA C22.2 No. 1010.1-92
Air Cleanliness Within
ISO 14644.1 Class 3, US Federal Standard 209E Class 1 / M1.5, AS 1386 Class 1.5, JIS B9920 Class 3, BS5295 Class C,
Working Area
Class M10,000 as per KS 27030.1 and other equivalent cleanliness classifications of the VDI 2083 and AFNOR X44101
Downflow
ULPA filter with integral metal guards and filter frame gaskets; fully compliant with EN1822 and IEST-RP-CC001.3 requirements
Main Filter
Type
Exhaust (x2) ULPA filters with integral metal guards and filter frame gaskets; fully compliant with EN1822 and IEST-RP-CC001.3 requirements
Minimum: 99.997% at 0.3µm / 99.988% at 0.12µm / 99.987% at MPPS
Downflow
Typical: 99.99927% at 0.3µm / 99.9977% at 0.12µm / 99.9972% at MPPS
Main Filter
Minimum: 99.99962% at 0.3µm / 99.99965% at 0.12µm / 99.99946% at MPPS
Efficiency
1st Exhaust
Typical: 99.999928% at 0.3µm / 99.99996% at 012µm / 99.999924% at MPPS
Ratings
Minimum: 99.99916% at 0.3µm / 99.9985% at 0.12µm / 99.9982% at MPPS
2nd Exhaust
Typical: 99.99983% at 0.3µm / 99.99979% at 012µm / 99.99971% at MPPS
Pre-Filter
Disposable and non-washable polyester fibers with 85% arrestance / EU3 rated
Typically <60 dBA at initial blower speed setting measured as per EN 12469:2000, based on 4 feet cabinet, subject to acoustic
Noise Level
properties of test environment
Light Intensity
>1700 lux / >158 foot candles, measured at work surface level (zero background) as per IEST-RP-CC002.2
Main Body Construction
1.5mmt / 0.06” / 16 gauge electro-galvanised steel with white oven-baked epoxy powder-coated finish
Front Window Construction
Colourless and transparent UV-absorbing 8mm / 0.31” tempered glass
Work Surface Construction
1.2mmt / 0.05” / 18 gauge stainless steel grade 304
220-240VAC
/ 50Hz 1Ph
110-130VAC
/ 60Hz 1Ph
Net Weight (Approximate)
Max Shipping Weight
Max Shipping Dimensions
(W x D x H)
Max Shipping Volume
Maximum
Power
Consumption
/Current
ESCO
®
1144W / 4.97A
1164W / 5.06A
1180W / 5.13A
1632W / 13.6A
1652W / 13.77A
1668W / 13.9A
351.5 kgs / 775 lbs
601 kgs / 1325 lbs
1995 x 1300 x 2750 mm
78.5” x 51.2” x 108.3”
7.13 cbm / 251.8 cbf
439.4 kgs / 969 lbs
751.2 kgs / 1656 lbs
2245 x 1300 x 2750 mm
88.4” x 51.2” x 108.3”
8.03 cbm / 283.6 cbf
527.3 kgs / 1163 lbs
901.5 kgs / 1988 lbs
2550 x 1300 x 2750 mm
100.4” x 51.2” x 108.3”
9.12 cbm / 322.07 cbf
Esco Biotechnology Equipment Division
Your local distributor:
Esco Biotech is a highly focused manufacturer of laminar flow, biohazard
safety and other HEPA-filtered cabinets for the laboratory with a history
of quality cabinets since 1978. We are predominantly oriented towards the
international marketplace, with sales in more than 70 countries and 90% of
turnover exported. Our products have been independently tested to standards such as AS1807.5 and EN12469. Products are manufactured under
an ISO 9001 registered quality system.
21 Changi South Street 1 · Singapore 486 777 · Tel: +65 6542 0833 · Fax: +65 6542 6920 · Email: [email protected] · biotech.escoglobal.com
ESCO Formalin Vaporizer for Decontamination
The Esco Formalin Vaporizer (FVP-00X) is a
microprocessor-controlled unit that simplifies and
automates the decontamination process for all sizes
and makes of biohazard safety cabinets. This also
increases safety for the decontamination operator and
laboratory personnel since no manual intervention is
required during the entire process.
The unit enables decontamination to be accomplished
by the standard protocol as recommended in EN12469
(European standard for microbiological safety cabinets)
with formalin and ammonia solutions. ANSI/NSF49
(American standard for Class II cabinets) recommends
decontamination using paraformaldehyde and
ammonium bicarbonate which is a similar protocol
but with agents in the solid (instead of liquid phase),
and as such this vapouriser can also be said to be
in compliance with the ANSI/NSF49 recommended
decontamination protocol.
ssorproce
o
r
c
i
M
olled
contr
Decontamination Cycle
 Operator starts process by pressing a
button on the touch pad
 Pre-delay timer allows decontamination
operator to seal up the cabinet before
vaporization of ammonia commences. Delay
period can be set from 1 to 59 minutes.
 Vaporization of formalin solution
commences to release the formaldehyde
gas to decontaminate the cabinet
 LCD display will show reminder for
operator to cycle the cabinet fans to ensure
even distribution of the gas
 Heater automatically cuts off when
formalin solution has completely boiled
off
 Contact time timer starts to regulate the
period for which the formaldehyde gas is
exposed to the cabinet internal surfaces for
the decontamination to take effect
 Neutralisation process begins
automatically to vaporize the ammonia
solution to neutralise the formaldehyde
gas
 LCD display will show reminder for
operator to cycle the cabinet fans to ensure
even distribution of the gas
 Neutralisation timer starts to regulate
the period for which the neutralisation
reaction takes place
General Construction Features
 Durable, heavy-duty full stainless steel
construction
 Electrical and electronic components
are isolated from the heating chamber to
prevent exposure to chemical fumes and
heat which can reduce component life
 Side mounted handles for easy
carrying
 Generously-sized formalin and ammonia
chambers (800mL maximum capacity in
total) which allows even the largest of
safety cabinets (up to 6 foot console units)
to be decontaminated with the maximum
agent volume recommended in EN12469
 Thermostats for the formalin and ammonia
chambers to prevent overheating
 Backup thermostat to ensure the
temperature of the external casing does
not rise above a safe level
 Increased diameter liquid chamber inlets
to allow the formalin and ammonia solutions
to be poured into the chambers more easily
without spillage
 Internal heat insulation to prevent cross
heating of chambers and damage to the
electrical and electronics components
 Manual shut-off isolating switch and
safety fuse
 Universal IEC power inlet design
(appropriate cordset must be specified on
order depending on country of use)
Control System Features
 The first and only microprocessor
controlled vaporizer unit in the world
specifically designed for safety cabinet
decontamination
 Automatic control of the entire
decontamination cycle .
 No manual intervention required for the
entire process
 In case of power failure, the vaporizer
will recover automatically once the power
resumes without any intervention
 Microprocessor unit has a built-in
watchdog and will self-reset in case of any
processor failure
 Pre-delay, contact time, neutralisation
timers are adjustable using the touch
control pad on the front of the unit
 Large, backlit, LCD display
 Audible and visual alarms for in case of
overheating during a cycle
 Password control to prevent
decontamination timers from being adjusted
by unauthorised personnel
 Easy to clean touch control panel without
any switches or manual relays to corrode
Ordering
 Esco Formalin Vaporizer is available
with the following electrical configurations.
Please ensure that the ordering code
corresponds to your electrical configuration
requirements when ordering.
Power Supply
Options
220-240VAC,
50HZ / 60HZ, 1 phase
100-130VAC,
50HZ / 60HZ, 1 phase
Ordering Codes
FV-001
FV-002
Esco Catalogue Revision No: FV.v1.10.2004 ~ Specifications subject to change without notice. ~ ©2004 Esco Micro Pte. Ltd.
ESCO
®
Esco Biotechnology Equipment Division
Your local distributor:
Esco Biotech is a highly focused manufacturer of laminar flow, biohazard
safety and other HEPA-filtered cabinets for the laboratory with a history
of quality cabinets since 1978. We are predominantly oriented towards the
international marketplace, with sales in more than 60 countries and 90% of
turnover exported. Our products have been independently tested to standards such as AS1807.5 and EN12469. Products are manufactured under
an ISO 9001 registered quality system.
21 Changi South Street 1 · Singapore 486 777 · Tel: +65 6542 0833 · Fax: +65 6542 6920 · Email: [email protected] · biotech.escoglobal.com
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