NISSUI Product Catalogue (日水產品目錄)

NISSUI Product Catalogue (日水產品目錄)
Apr. 2010's Edition of The “Nissui” Product Catalogue
No.1 in media market share of Japan.
( p.24, Market of Food Inspection 2009, Fuji Keizai Co., Ltd. )
Cat. Koh SAI
Index
Dehydrate Media
1
Salmonella
05006
05002
05009
05132
05130
05131
1
SBG Broth Base “Nissui” .......................................................
EEM Broth “Nissui” ................................................................
Selenite Cystine Broth Base “Nissui” ..................................
Tetrathionate Broth (TT) “Nissui” .........................................
Rappaport-Vassiliadis Broth (RV) “Nissui” .........................
Buffered Peptone Water (BPW) “Nissui” ............................
Enterobacteria Separation
05025
05024
05032
05033
05021
05020
05040
05042
05037
05103
05140
05041
1-1
1-2
1-3
1-4
1-5
1-6
2
SSB Agar “Nissui”
SSB Agar “Nissui” ..................................................................
SS Agar with Sucrose “Nissui”
SS Agar with Sucrose “Nissui” .............................................
SS Agar “Nissui”
SS Agar “Nissui” .....................................................................
DHL Agar “Nissui” ..................................................................
BTB Lactose Agar “Nissui” ...................................................
MacConkey Agar “Nissui” .....................................................
TSI Agar “Nissui” ....................................................................
X-SAL Agar “Nissui” ...............................................................
MLCB Agar “Nissui” ...............................................................
2-1
2-2
2-3
2-4
2-5
2-6
2-7
2-8
2-9
Enterobacteria Confirmation, Differentation and Identification3
05104
05106
05148
05111
05107
05182
05180
06626
06615
06616
06628
LIM Agar “Nissui” ...................................................................
SIM Agar “Nissui” ...................................................................
DNA Agar “Nissui” ..................................................................
SC Agar “Nissui” .....................................................................
VP-MR Medium “Nissui” ........................................................
ONPG Disk “Nissui” ...............................................................
Cytochrome Oxidase Test Strip “Nissui” .............................
ID Test・EB-20 “Nissui”
ID Test・EB-20 “Nissui” EB Plate
ID Test・EB-20 “Nissui” EB Broth
ID Test・EB-20 “Nissui” EB Reagent ..................................
3-1
3-2
3-3
3-4
3-5
3-6
3-7
3-8
i
Index-i-20100119 Koh SAI
Index
Dehydrate Media
1
Enterobacteria Confirmation, Differentation and Identification1
06673
06671
06608
08720
08721
ID Test・EB-9 “Nissui”
ID Test・EB-9 “Nissui” EB9 Reagent ................................. 3-9
Sterilized Oil Paraffin “Nissui” ......................................... 3-10
Quick ID GN “Nissui”
Quick ID GN “Nissui” Reagent .......................................... 3-11
Vibrio parahaemolyticus, cholera
05215
05206
05201
05208
05204
05135
Salt Polymyxin Broth “Nissui” ..............................................
Alkaline Peptone Water “Nissui” .........................................
Vibrio Agar “Nissui” ...............................................................
PMT Agar Base “Nissui” ........................................................
Thiosulfate citrate bile Saccharose Agar “Nissui” ...........
X-VP Agar “Nissui” .................................................................
Non-fermentative Gram-negative rod
05220
05221
06629
06617
06618
06631
4-1
4-2
4-3
4-4
4-5
4-6
5
NAC Agar “Nissui” .................................................................. 5-1
Cetrimide Agar “Nissui” ........................................................ 5-2
ID Test・NF-18 “Nissui”
ID Test・NF-18 “Nissui” NF Plate
ID Test・NF-18 “Nissui” NF Broth
ID Test・NF-18 “Nissui” NF Reagent .................................... 5-3
Staphylococci
05234
05236
05246
05238
06637
06613
06614
06638
4
6
Staphylococcus Medium No. 110 “Nissui” ........................ 6-1
Mannitol Salt Agar “Nissui” .................................................. 6-2
PEA Agar “Nissui” .................................................................. 6-3
Salt Egg Yolk Agar Base “Nissui” ........................................ 6-4
N-ID Test・SP-18 “Nissui”
N-ID Test・SP-18 “Nissui” SP Plate
N-ID Test・SP-18 “Nissui” SP Broth
N-ID Test・SP-18 “Nissui” SP Reagent .............................. 6-5
Haemophilus / Neisseria
7
06635 ID Test・HN-20 Rapid “Nissui”
06640 ID Test・HN-20 Rapid “Nissui” HN Reagent ..................... 7-1
ii
Index-ii-20100119 Koh SAI
Index
Dehydrate Media
1
Anaerobes
05420
05426
05433
05422
05460
05424
05404
05405
05440
05441
05450
05430
05409
8
GAM Agar “Nissui” ................................................................. 8-1
GAM Agar, Modified “Nissui” ............................................... 8-2
GAM Broth, Modified “Nissui” .............................................. 8-3
GAM Broth “Nissui” ................................................................ 8-4
GAM Semisolid without Dextrose “Nissui” ......................... 8-5
GAM Semisolid “Nissui” ........................................................ 8-6
CW Agar Base without KM “Nissui” .................................... 8-7
CW Agar Base with KM “Nissui” .......................................... 8-8
Bacteroides Agar “Nissui” .................................................... 8-9
FM Agar, Modified “Nissui” ............................................... 8-10
GAM Agar with GM “Nissui” .............................................. 8-11
BL Agar “Nissui” ................................................................. 8-12
Clostridia Count Agar “Nissui” ......................................... 8-13
Bacillus cereus
9
05282 NGKG Agar Base “Nissui” ..................................................... 9-1
Viable bacteria
05530
05533
05534
05503
05505
05507
05509
05514
05511
05516
05630
05528
05522
05527
05601
10
Sensitivity Disk Agar-N “Nissui” ....................................... 10-1
Mueller-Hinton Agar-N “Nissui” ........................................ 10-2
Sensitivity Test Broth “Nissui” .......................................... 10-3
Heart Infusion Agar “Nissui” ............................................ 10-4
Heart Infusion Broth “Nissui” ........................................... 10-5
Brain Heart Infusion Agar “Nissui” .................................. 10-6
Brain Heart Infusion Broth “Nissui” ................................. 10-7
Nutrient Agar “Nissui” ....................................................... 10-8
Nutrient Broth “Nissui” ...................................................... 10-9
Trypto-Soya Agar (SCD Agar) “Nissui” ......................... 10-10
Trypto-Soya Broth (SCD Broth) “Nissui” ....................... 10-11
Buffered Sodium Chloride Peptone Solution (pH7.0) “Nissui” 10-12
Dorset Egg Medium “Nissui” ........................................ 10-13
CLED Agar “Nissui” ......................................................... 10-14
TGC Medium, Fluid “Nissui” .......................................... 10-15
iii
Index-iii-20100119 Koh SAI
Index
Dehydrate Media
1
Viable bacteria
05610
05629
05602
05618
05622
05625
1
TGC Medium without Indicator, Liquid “Nissui” .........
TGC Medium without Indicator, Fluid “Nissui” ...........
Dextrose Peptone Broth “Nissui” .................................
Standard Method Agar “Nissui” ...................................
Plate Count Agar with BCP “Nissui” ............................
CVT Agar “Nissui” ...........................................................
E.coli and Coliform
05634
05638
05648
05636
05644
05631
05632
05607
05591
05593
05613
06574
06516
05617
06517
05643
05679
05649
11
Lactose Broth “Nissui” ....................................................... 11-1
BGLB Broth “Nissui” ........................................................... 11-2
EC Broth “Nissui” ................................................................ 11-3
Desoxycholate Agar “Nissui” ............................................ 11-4
EMB Agar “Nissui” .............................................................. 11-5
X-GAL Agar “Nissui” ........................................................... 11-6
XM-G Agar “Nissui” ............................................................ 11-7
Blue Light Broth “Nissui” ................................................... 11-8
EC-Blue 100P “Nissui”
EC-Blue 100 “Nissui”
EC-Blue 10 “Nissui” ............................................................ 11-9
EC-Blue 100 Bottle Holder “Nissui” .............................. 11-10
EC-Blue 10 “Nissui” Comparator
EC-Blue 100 “Nissui” Comparator ................................ 11-11
EC-Blue 100 “Nissui” MPN Plate .................................. 11-12
MacConkey Sorbitol Agar “Nissui” ............................... 11-13
EF Agar Base “Nissui” .................................................... 11-14
mEC Broth “Nissui” ........................................................ 11-15
Fungus
05701
05702
05680
05703
05705
10-16
10-17
10-18
10-19
10-20
10-21
12
Sabouraud Agar “Nissui” ..................................................
Corn Meal Agar “Nissui” ....................................................
AC Broth Base “Nissui” ......................................................
Candida GE Agar “Nissui” .................................................
Czapek Dox Agar “Nissui” .................................................
12-1
12-2
12-3
12-4
12-5
iv
Index-iv-20100119 Koh SAI
Index
Dehydrate Media
1
Fungus
12
05706 Malt Agar “Nissui” .............................................................. 12-6
05709 Potato Dextrose Agar “Nissui” ......................................... 12-7
Vitamin
13
05800
05801
05802
05803
05819
05815
05816
05814
05817
05818
05835
Lactobacilli Culture Agar “Nissui” .................................... 13-1
Lactobacilli Inoculum Broth “Nissui” ............................... 13-2
B12 Culture Agar “Nissui” .................................................. 13-3
B12 Inoculum Broth “Nissui” ............................................. 13-4
B12 Assay Medium (Set) “Nissui” ..................................... 13-5
Pyridoxine Assay Medium “Nissui” .................................. 13-6
Niacin Assay Medium “Nissui” ......................................... 13-7
Folic Acid Assay Medium “Nissui” ................................... 13-8
Pantothenate Assay Medium “Nissui” ............................ 13-9
Biotin Assay Medium “Nissui” ...................................... 13-10
Powdered Agar “Nissui” ................................................ 13-11
Prepared Media
2
Tube Media
14
05169 Nissui Tube Triple Sugar Iron Agar ................................. 14-1
05170 Nissui Tube Sulfide Indole Motility Medium .................. 14-2
05171 Nissui Tube Lysine Indole Motility Medium ................... 14-3
Food Stamp
06053
06052
06764
06763
06051
06050
06776
06775
06055
15
Food Stamp “Nissui” Desoxycholate Agar (DESO)
Food Stamp “Nissui” Desoxycholate Agar (DESO) ........
Food Stamp “Nissui” X-GAL Agar (XGAL)
Food Stamp “Nissui” X-GAL Agar (XGAL) ........................
Food Stamp “Nissui” Standard Method Agar (SMA)
Food Stamp “Nissui” Standard Method Agar (SMA) .....
Food Stamp “Nissui” XM-G Agar (XM-G)
Food Stamp “Nissui” XM-G Agar (XM-G) .........................
Food Stamp “Nissui” TCBS Agar (TCBS) .........................
15-1
15-2
15-3
15-4
15-5
v
Index-v-20100119 Koh SAI
Index
Prepared Media
1
Food Stamp
15
06054
06057
06056
06751
06750
06753
06752
06757
06756
06064
06063
06755
Food Stamp “Nissui” TCBS Agar (TCBS) ......................... 15-5
Food Stamp “Nissui” TGSE Agar (TGSE)
Food Stamp “Nissui” TGSE Agar (TGSE) ......................... 15-6
Food Stamp “Nissui” MLCB Agar (MLCB)
Food Stamp “Nissui” MLCB Agar (MLCB) ....................... 15-7
Food Stamp “Nissui” Cereus Agar (CERE)
Food Stamp “Nissui” Cereus Agar (CERE) ...................... 15-8
Food Stamp “Nissui” X-SA Agar (X-SA)
Food Stamp “Nissui” X-SA Agar (X-SA) ........................... 15-9
Food Stamp “Nissui” Sabouraud Agar (SABO)
Food Stamp “Nissui” Sabouraud Agar (SABO) .......... 15-10
Food Stamp “Nissui”
Potato dextrose Agar with Chloramphenicol (PDA)
06754 Food Stamp “Nissui”
Potato dextrose Agar with Chloramphenicol (PDA) . 15-11
06231 Food Plate X “Nissui” ..................................................... 15-12
Compact Dry (Link to Product's Info (English, Traditional or Simplified Chinese))
16
06740 Compact Dry “Nissui” TC
16-1
06741 Compact Dry “Nissui” TC ................................................... 16-1
06744 Compact Dry “Nissui” CF
06745 Compact Dry “Nissui” CF ................................................... 16-2
06742 Compact Dry “Nissui” EC
06743 Compact Dry “Nissui” EC ................................................... 16-3
06746 Compact Dry “Nissui” YM
06747 Compact Dry “Nissui” YM .................................................. 16-4
06748 Compact Dry “Nissui” VP
06749 Compact Dry “Nissui” VP ................................................... 16-5
06729 Compact Dry “Nissui” X-SA
06730 Compact Dry “Nissui” X-SA ............................................... 16-6
06732 Compact Dry “Nissui” SL
06733 Compact Dry “Nissui” SL .................................................. 16-7
vi
Index-vi-20100119 Koh SAI
Index
Prepared Media
1
Compact Dry
16
06727
06728
06738
01553
01551
06737
06598
06472
Compact Dry “Nissui” X-BC
8-1
Compact Dry “Nissui” X-BC ............................................... 16-8
Easy Wiping Kit “Nissui” ................................................... 16-9
Sterilized Diluting Solution “Nissui” 9 mL × 4
Sterilized Diluting Solution “Nissui” 90 mL ................ 16-10
Opener with Sterilized Diluting Solution “Nissui” ...... 16-11
Wiping Frame “Nissui” 100 .......................................... 16-12
Sterilized Spoid “Nissui” ................................................ 16-13
Clean Stamp
17
06781
06780
06785
06784
06787
Clean Stamp “Nissui” MSO Agar (MSO)
Clean Stamp “Nissui” MSO Agar (MSO) ......................... 17-1
Clean Stamp “Nissui” SCD Agar (SCD)
Clean Stamp “Nissui” SCD Agar (SCD) ........................... 17-2
Clean Stamp “Nissui”
SCD Agar with Lecithin, polysorbate 80 (SCDLP)
06786 Clean Stamp “Nissui”
SCD Agar with Lecithin, polysorbate 80 (SCDLP) ........ 17-3
06783 Clean Stamp “Nissui” Mannitol Salt Agar with Egg Yolk (MSEY)
06782 Clean Stamp “Nissui” Mannitol Salt Agar with Egg Yolk (MSEY) ... 17-4
06791 Clean Stamp 25 “Nissui” SCD Agar (SCD)
06790 Clean Stamp 25 “Nissui” SCD Agar (SCD) ..................... 17-5
06789 Clean Stamp “Nissui” Cetrimide Kanamycin Sulfate
Nalidixic Acid Agar (PSEU) .............................................. 17-6
06793 Clean Stamp 25 “Nissui” SCDLP Agar (SCDLP)
06792 Clean Stamp 25 “Nissui” SCDLP Agar (SCDLP) ............ 17-7
06795 Clean Stamp 25 “Nissui”
Sabouraud Agar with Chloramphenicol (CPSB)
06794 Clean Stamp 25 “Nissui”
Sabouraud Agar with Chloramphenicol (CPSB) ........... 17-8
vii
Index-vii-20100119 Koh SAI
Index
Food Allergen Detection Kit
08630
08631
08632
08633
08634
08615
08616
08617
08618
08619
08606
08621
08622
08623
FASTKIT SLIM EGG “Nissui”
FASTKIT SLIM MILK “Nissui”
FASTKIT SLIM WHEAT “Nissui”
FASTKIT SLIM BUCK WHEAT “Nissui”
FASTKIT SLIM PEANUT “Nissui” .......................................
FASTKIT ELISA ver.II EGG “Nissui”
FASTKIT ELISA ver.II MILK “Nissui”
FASTKIT ELISA ver.II WHEAT “Nissui”
FASTKIT ELISA ver.II BUCK WHEAT “Nissui”
FASTKIT ELISA ver.II PEANUT “Nissui”
FASTKIT ELISA ver.II SOYBEAN “Nissui” ..........................
FA test EIA-Crustacea “Nissui”
FA test Extraction Solution “Nissui” .............................
FA test Immunochromato-Crustacea “Nissui” ...........
18
18-1
18-2
18-3
18-4
Tissue Culture Medium
05900
08160
05901
05902
05904
05913
08190
08192
05908
05915
05919
05963
05911
05918
05971
Eagle’s MEM “Nissui” ①
Eagle’s MEM “Nissui” ① ................................................... 19-1
Eagle’s MEM “Nissui” ② ................................................... 19-2
Eagle’s MEM “Nissui” ③ ................................................... 19-3
Eagle’s MEM Amino Acids and Vitamins Medium “Nissui” 19-4
Dulbecco’s PBS (-) “Nissui”
Dulbecco’s PBS (-) “Nissui”
Dulbecco’s PBS (-) “Nissui” ............................................... 19-5
Glutamine “Nissui” ............................................................ 19-6
Dulbecco’s Modified Eagle Medium “Nissui” ① ........... 19-7
Dulbecco’s Modified Eagle Medium “Nissui” ② ........... 19-8
SFM-101 “Nissui” ............................................................... 19-9
RPMI 1640 Medium “Nissui” ① .................................. 19-10
RPMI 1640 Medium “Nissui” ② ................................. 19-11
ES Medium “Nissui” ....................................................... 19-12
viii
Index-viii-20100119 Koh SAI
Index
Tissue Culture Medium
05909
05910
05905
05906
Medium 199 “Nissui” ....................................................
Ham’s F12 Medium “Nissui” ........................................
Hank’s solution “Nissui” ①
Hank’s solution “Nissui” ② ..........................................
18
19-13
19-14
19-15
Company Information
Established :
April 6, 1935
Head Office :
3-23-9, Ueno, Taito-ku, Tokyo 110-8736
Capital :
4,449,844,000 yen
Employees :
334 (2010.3.31)
Associated Company : Nippon Suisan Kaisha, Ltd.
Distribution :
Culture Media and Diagnostics
Pharmaceuticals and Health Food Supplements
Export To … :
Germany, Thailand, Korea, Taiwan, Hong
Kong, China, United States, Switzerland, ...
URL :
http://www.nissui-pharm.co.jp/
Contact
DIAGNOSTIC AGENT MARKETING DEPARTMENT
INTERNATIONAL SALES GROUP
TEL: +81 - 3 - 5846 - 5701 FAX: +81 - 3 - 5846 - 5629
In English: Mikiko NISHIZAWA (E-mail: m-nishizawa@nissui-pharm.jp)
In Chinese: Koh SAI (E-mail: k-sai@nissui-pharm.jp)
ix
Index-viii-20100119 Koh SAI
SBG Broth Base “Nissui”
(Selenite Brilliant Green Broth Base, For isolation of Salmonella)
Uninoculated
Salmonella
Code
Form
Package
Storage
Shelf Life
05006
Powder
100 g
Dry, Cool
2 years
in the medium.
SBG Sulfa Medium is more suitable than
this medium for the isolation of Salmonella
from eggs and egg products.
Directions
Suspend 19.7 g of the dehydrated medium
and 4.0 g of sodium selenite in 1,000 mL of
distilled water, mix well to dissolve the
medium. Avoiding excessive heating, keep
at 100°C for 10 minutes. Do not
autoclave. Distribute aseptically about 10
mL amounts in to sterilized test tubes.
Inoculate about 1 g of the specimen such
as feces. Mix well and incubate at 37°C for
18 – 24 hours. Take a loopful of the culture
and then inoculate on a plate of the
selective medium.
Use sodium selenite of high quality.
Storage
Keep dry and cool place to prevent powder
product and prepared solution form color
fading.
Formula
Components
Yeast Extract
Peptone
Mannitol
Sodium Taurocholate
Dipotassium Phosphate
Potassium Dihydrogen
Phosphate
Brilliant Green
Remarks
The medium inhibits the growth of
Alcaligenes, Pseudomonas, Proteus and
other bacteria that grow on the selenite
medium, and permits the growth of
Salmonella.
S. typhi and S. paratyphi A do not grow well
In 19.7 g/L
5.0 g
5.0 g
5.0 g
1.0 g
2.65 g
1.02 g
0.005 g
pH 7.2 ±
Back to the Index
1-1
05006-20100120 Koh SAI
EEM Broth “Nissui”
(Enterobacteriaceae Enrichment Mannitol Broth for preliminary culture of Salmonella)
Uninoculated
Salmonella
Code
Form
Package
Storage
Shelf Life
05002
Powder
100 g
Dry, RT
3 years
Directions
Formula
Suspend 43.5 g of the dehydrated medium
in 1,000 mL of distilled water, mix well to
dissolve the medium. Distribute adequate
amounts into sterilized test tubes or
sterilized flask and then heat them at
100°C for 30 minutes. Avoid excessive
heating, do not autoclave.
Add the specimen of about one-tenth of the
medium and incubate at 37°C for 3 hours.
Stir them well after the first incubation and
continue the incubation for 20 – 24 hours
or more. Finally, take a loopful of the
culture and subculture on an adequate
selective medium.
Components
In 43.5 g/L
Peptone
10.0 g
Mannitol
5.0 g
Disodium Phosphate
Potassium Dihydrogen
Phosphate
Bile Powder
6.45 g
2.0 g
20.0 g
Brilliant Green
0.0135 g
pH 7.2 ±
Back to the Index
Remarks
The medium is quite suitable for preliminary
culture of Salmonella in food and feed.
1-2
05002-20100120 Koh SAI
Selenite Cystine Broth Base “Nissui”
(Selective Enrichment Medium for Salmonella)
Code
Form
Package
Storage
Shelf Life
05009
Powder
100 g
Dry, RT
3 years
Directions
Formula
Components
Suspend 19.0 g of the dehydrated medium
and 4.0 g of Sodium Selenite in 1,000 mL
of distilled water, mix well to dissolve the
medium. Heat the mixture to dissolve, if
necessary. Do not autoclave, nor heat too
long.
The medium can be used for selective
growth culture of Salmonella in not only
clinical specimen but also foodstuffs and
water.
In 19.0 g/L
Peptone
5.0 g
Lactose
4.0 g
Sodium Phosphate
10.0 g
L-Cystine
0.01 g
pH 7.0 ±
Back to the Index
Remarks
The medium is ready to use simply after
mixing to dissolve.
If not dissolved well, heat up.
If it is heated too long, a red turbidity is
produced.
1-3
05009-20100122 Koh SAI
Tetrathionate Broth (TT) “Nissui”
Code
Form
Package
Storage
Shelf Life
05132
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 46.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Cool to below
45°C. Add 20 mL iodine solution (6 g iodine
crystals and 5 g potassium iodide dissolved
in 20 mL of distilled water) and mix well.
Distribute adequate amounts into sterilized
test tubes. Do not heat after addition of
iodine solution.
Prepared medium should be used in a day
of preparation. Incubate at 42°C for 20 24 hours.
Components
In 46.0 g/L
Casein Peptone
2.5 g
Meat Peptone
2.5 g
Bile Salt
1.0 g
Calcium Carbonate
10.0 g
Sodium Thiosulfate
30.0 g
Back to the Index
Remarks
The medium is suitable for enrichment of
Salmonella in food.
1-4
05132-20100122 Koh SAI
Rappaport-Vassiliadis (RV) Broth “Nissui”
(Selective isolation medium for Salmonella)
Uninoculated
Salmonella
Code
Form
Package
Storage
Shelf Life
05130
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 26.8 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute
adequate amounts into sterilized test tubes
or sterilized flask and then autoclave at
115°C for 15 minutes.
Incubate at 30-35°C for 18–24 hours.
Components
In 26.8 g/L
Soy Bean Peptone
4.5 g
Sodium Chloride
7.2 g
Magnesium Chloride
Remarks
The medium is suitable for enrichment of
Salmonella in food.
13.58 g
Dipotassium Phosphate
0.18 g
Potassium Dihydrogen
Phosphate
1.26 g
Malachite green
0.036 g
pH 5.2 ± 0.2
Back to the Index
1-5
05130-20100126 Koh SAI
Buffered Peptone Water (BPW) “Nissui”
(Buffered Peptone Water for Pre-culture of Salmonella)
Code
Form
Package
Storage
Shelf Life
05131
Powder
300 g
Dry, RT
3 years
Salmonella in food.
Directions
Suspend 20.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute
adequate amounts into sterilized test tubes
or sterilized flask and then autoclave at
121°C for 15 minutes.
Add 1/10 volume of the homogenized
specimen (i.e., 1 mL homogenized
specimen put into 10 mL broth) into the
broth and stir them well. Incubate at 37°C
for 20 – 24 hours. Finally, take one loop of
the culture and subculture on an adequate
selective medium (ex. Compact Dry SL, SS
agar, MLCB Agar).
For liquid Egg sample, add 0.2 g L-cystein or
64 mg FeSO4・7H2O in 1 liter of Broth.
Formula
Components
In 20.0 g/L
Peptone
10.0 g
Sodium Chloride
5.0 g
Monopotassium
Phosphate
3.5 g
Potassium Dihydrogen
Phosphate
1.5 g
pH 7.2 ±
Back to the Index
Remarks
The medium is suitable for pre-culture of
1-6
05131-20100126 Koh SAI
SSB Agar “Nissui”
(Salmonella Shigella Brom Cresol Purple Agar)
Code
05025
05024
Form
Package
Storage
Shelf Life
Dry, RT
3 years
300 g
Granule
60 g × 20
turbid colonies.
The medium strongly supports the growth of Salmonella, and
even S. pullorum and S. enteritidis, which hardly grow on SS agar,
grow well on this medium.
There is no need to inoculate the specimen heavily as in case of
SS Agar.
Directions
Suspend 60.0 g of the dehydrated medium in 1,000 mL of
distilled water, mix well and heat to dissolve the medium.
Distribute into Petri dishes without sterilizing and dry the surface
of the plate sufficiently before use. Incubate at 37°C for 16 – 18
hours.
Formula
Determinations
Components
Meat Extract
Peptone
Sodium Citrate
Sodium Thiosulfate
Ferric Ammonium Citrate
Lactose
Sucrose
Bile Salts
Neutral Red
Brom Cresol Purple
Agar
Organisms non-fermenting lactose and saccharose such as
Salmonella and Shigella form light blue, semitransparent
colonies.
On the other hand, organisms fermenting lactose and saccharose
form purple or pink, turbid colonies, which makes differentiation
between two types of colonies easy. Organisms such as Proteus
fermenting saccharose form light purple colonies, while the
colonies of organisms producing hydrogen sulfide turn black more
easily than those formed on SS Agar.
Remarks
The medium contains the combination of two dyes, i.e. neutral red
and brom cresol purple, so that the plate assumes a reddish
purple color. Thus the medium is distinctive in its usage from SS
Agar, DCLS Agar and MacConkey Agar.
Salmonella and Shigella form light blue, semitransparent colonies
on the medium larger than those on SS agar in a short time of
incubation (16 – 18 hours). On the other hand, organisms
fermenting lactose and saccharose form vividly reddish purple,
2-1
In 60.0 g/L
3.0 g
8.0 g
5.0 g
2.0 g
1.0 g
10.0 g
10.0 g
5.0 g
0.02 g
0.01 g
16.0 g
pH 7.4 ±
Back to the Index
05025-05024-20100125 Koh SAI
SS Agar with Sucrose “Nissui”
(Selective isolation and differential medium for Salmonella and Shigella)
Code
05032
05033
Form
Package
Storage
Shelf Life
Dry, RT
3 years
280 g
Granule
70 g × 20
media.
●Note
The medium requires inoculation of a large amount of
feces than in case of other selective media.
Directions
Suspend 70.0 g of the dehydrated medium in 1,000 mL of
distilled water, mix well and heat to dissolve the medium.
Distribute about 20 mL amounts to Petri dishes. Dry the
surface of the plate until moisture disappears from the
surface by leaving the lids of the dishes partly open.
Do not autoclave.
Formula
Components
Meat Extract
Bile Salts
Peptone
Lactose
Sucrose
Sodium Citrate
Sodium Thiosulfate
Ferric Citrate
Neutral Red
Brilliant Green
Agar
Determinations
Inoculate the specimens heavily and incubate at 37°C for
20 – 24 hours.
Pathogenic bacteria form semitransparent colonies
Lactose fermenters form pink or red colonies. Also
Sucrose fermented Citrobacter does not form black
colonies and it can be identify easily.
Remarks
Features
The medium inhibits the growth of E. coli and
miscellaneous bacteria.
The medium does not require any sterilizing procedures.
The medium enables to detect pathogenic bacteria easily
and its detection rate is far more excellent than other
●
In 70.0 g/L
5.0 g
8.5 g
8.0 g
10.0 g
10.0 g
8.5 g
5.5 g
1.0 g
0.025 g
0.33 mg
13.5 g
pH 7.2 ±
Back to the Index
2-2
05032-05033-20100125 Koh SAI
SS Agar “Nissui”
(Selective isolation and differential medium for Salmonella and Shigella)
Code
05021
05020
Form
Package
Storage
Shelf Life
Dry, RT
3 years
240 g
Granule
60 g × 20
and its detection rate is far more excellent than other
media.
●Note
The medium requires inoculation of a large amount of
feces than in case of other selective media.
Directions
Suspend 60.0 g of the dehydrated medium in 1,000 mL of
distilled water, mix well and heat to dissolve the medium.
Distribute about 20 mL amounts to Petri dishes. Dry the
surface of the plate until moisture disappears from the
surface by leaving the lids of the dishes partly open.
Do not autoclave.
Formula
Components
Meat Extract
Bile Salts
Peptone
Lactose
Sodium Citrate
Sodium Thiosulfate
Ferric Citrate
Neutral Red
Brilliant Green
Agar
Determinations
Inoculate the specimens heavily and incubate at 37°C for
20 – 24 hours.
Pathogenic bacteria form semitransparent colonies
Lactose fermenters form pink or red colonies. Some
strains of Proteus produce hydrogen sulfide and turn
black. Proteus grown on the medium forms colonies
similar to those of pathogenic bacteria.
Remarks
Features
The medium inhibits the growth of E. coli and
miscellaneous bacteria.
The medium does not require any sterilizing procedures.
The medium enables to detect pathogenic bacteria easily
●
In 60.0 g/L
5.0 g
9.0 g
7.5 g
10.0 g
8.5 g
5.5 g
1.0 g
0.025 g
0.33 mg
13.5 g
pH 7.3 ±
Back to the Index
2-3
05021-05020-20100125 Koh SAI
DHL Agar “Nissui”
(Desoxycholate Hydrogen Sulfide Lactose Agar)
Code
Form
Package
Storage
Shelf Life
05040
Granule
300 g
Dry, RT
3 years
Directions
Remarks
Suspend 63.3 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute
about 20 mL amounts to the Petri dishes
and dry the surface of the medium before
use.
Though this medium has little selectivity, it
supports excellent growth of Shigella and
Salmonella that do not grow well on SS
Agar, and is used for the isolation of
pathogenic coli.
Formula
Determinations
Components
Meat Extract
Peptone
Lactose
Sucrose
Sodium Desoxycholate
Sodium Thiosulfate
Sodium Citrate
Ferric Ammonium Citrate
Neutral Red
Agar
Incubate at 37°C for about 20 hours. Avoid
heavy inoculation of the specimen as on SS
Agar. The amount of specimen applied to in
case of MacConkey Agar is desirable.
Lactose, saccharose non-fermenting
organisms form colorless and transparent
colonies relatively larger than those on SS
Agar. On the other hand, lactose,
saccharose fermenting organisms form red
and opaque colonies. Thus differentiation
between the two organisms is easy.
Organisms producing hydrogen sulfide are
liable to form black colonies.
In 63.3 g/L
3.0 g
20.0 g
10.0 g
10.0 g
1.0 g
2.3 g
1.0 g
1.0 g
0.03 g
15.0 g
pH 7.4 ±
Back to the Index
2-4
05040-20100126 Koh SAI
BTB Lactose Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05042
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 40.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute
about 20 mL amounts into the Petri Dish
after being sterilized by autoclaving at
121°C for 15 minutes.
Incubate at 37°C for about 18-24 hours.
Since the medium has a very weak
selectivity, small colonies of Staphylococcus
and/or gram positive Cocci may grow.
Components
In 40.0 g/L
Meat Extract
5.0 g
Peptone
10.0 g
Lactose
10.0 g
Brom Thymol Blue
0.08 g
Agar
15.0 g
pH 7.2 ±
Remarks
Back to the Index
Organisms non-fermenting lactose such as
Salmonella and Shigella form colorless
semitransparent colonies, while organisms
fermenting lactose such as coliform form
yellow colonies.
2-5
05042-20100126 Koh SAI
MacConkey Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05037
Granule
300 g
Dry, RT
3 years
is easier than in case of Endo’s or
Drigalski’s medium.Even some organisms
that cannot grow on SS Agar grow on the
medium.
●Note:
●Aseptic procedures should be taken for this
medium contrary to those of SS Agar.
Directions
Suspend 50.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes, and
distribute about 20 mL into Petri dishes
aseptically, and dry the surface of the
medium before use.
Formula
Determinations
Components
Peptone
Bile Salts
Lactose
Sodium Chloride
Neutral Red
Crystal Violet
Agar
Incubate at 30-35°C for 18–72 hours.
Pathogenic bacteria form semitransparent
colonies.
Colonies of E. coli assume an intensely redbrick
color and produce light pink or redbrick
sediments around.
Remarks
Features:
Differentiation between pathogenic bacteria
and nonpathogenic bacteria on the medium
●
In 50.0 g/L
20.0 g
2.0 g
10.0 g
5.0 g
0.03 g
0.001 g
13.0 g
pH 7.1 ± 0.2
Back to the Index
2-6
05037-20100204 Koh SAI
TSI Agar “Nissui”
(Triple Sugar Iron Agar)
Code
Form
Package
Storage
Shelf Life
05103
Powder
100 g
Dry, RT
3 years
indicates the colonies are those of V. cholerae).
Directions
Remarks
Suspend 61.4 g of the dehydrated medium in 1,000
mL of distilled water, mix well and heat to dissolve
the medium. Distribute about 4 mL into small test
tubes, sterilize by autoclaving at 121°C for 15
minutes. Solidify in a slanted position with a
generous butt. (Add 3 – 4% NaCl for vibrios)
The medium is a composite medium for the
differentiation of Enterobacteriaceae and vibrios,
and permits simultaneous observation of
fermentation of dextrose, lactose and saccharose,
gas production and the hydrogen sulfide production.
Determinations
Formula
Take the bacteria to be tested with a straight wire,
stab into the butt and smear on the slant. Incubate
at 37°C for 18-24 hours before interpreting the
reaction.
●Saccharolytic ability
Dextrose fermenting organisms show the following
changes; the butt turns yellow, while the slant shows
no change of color. Organisms fermenting both or
either of lactose and saccharose change the color of
both the butt and slant into yellow.
●Gas production
If gas is produced, bubbles or cracks will be made in
the butt, and the production of hydrogen sulfide
changes the butt into black.
Addition of cytochrome oxidase test reagent by
dropping it onto the colonies causes them to turn
blue within a minute or two (the positive reaction
Components
Meat Extract
Sodium Chloride
Peptone
Lactose
Sucrose
Dextrose
Ferric Citrate
Sodium Thiosulfate
Phenol Red
Agar
In 61.4 g/L
5.0 g
5.0 g
15.0 g
10.0 g
10.0 g
1.0 g
0.2 g
0.2 g
0.02 g
15.0 g
pH 7.3 ±
Back to the Index
2-7
05103-20100127 Koh SAI
X-SAL Agar “Nissui”
(For isolation of Salmonella causing food poisoning)
Code
Form
Package
Storage
Shelf Life
05140
Granule
300 g
Dry, RT
3 years
Note
The medium requires inoculation of a large amount
of feces than in case of other selective media.
Directions
●
Suspend 68.2 g of the dehydrated medium in 1,000
mL of distilled water, mix well and heat to dissolve
the medium. Distribute about 20 mL amounts to
Petri dishes, and dry the surface of the medium
before use. Do not autoclave.
Formula
Components
Peptone
Meat Extract
Yeast Extract
Lactose
Saccharose
L-Lysine Hydrochlorude
Sodium Thiosulfate
Ammonium Iron Citrate
Sodium Citrate
Bile Salt
Neutral Red
Colorimetric Substrate by
Enzyme
Agar
Determinations
Inoculate the specimens heavily and incubate at 3337°C for 18-24 hours.
Pathogenic bacteria form semitransparent colonies
Lactose fermenters form pink or red colonies. Some
strains of Proteus produce hydrogen sulfide and turn
black. Proteus grown on the medium forms colonies
similar to those of pathogenic bacteria.
Remarks
Features
The medium inhibits the growth of E. coli and
miscellaneous bacteria.
The medium does not require any sterilizing
procedures.
The medium enables to detect pathogenic bacteria
easily and its detection rate is far more excellent
than other media.
●
2-8
In 68.2 g/L
18.5 g
2.5 g
1.0 g
10.0 g
10.0 g
5.0 g
2.0 g
1.0 g
1.0 g
2.0 g
0.03 g
0.2 g
15.0g
pH 7.0 ±
Back to the Index
05140-20100127 Koh SAI
MLCB Agar® “Nissui”
(For isolation of Salmonella causing food poisoning)
Code
Form
Package
Storage
Shelf Life
05041
Powder
300 g
Dry, RT
3 years
Directions
Remarks
Suspend 49.0 g of the dehydrated medium in
1,000 mL of distilled water, mix well and heat
to dissolve the medium. Distribute about 20
mL into Petri dishes without sterilizing and
dry the surface of the medium sufficiently
before use.
The medium is not suitable for an isolation of
Salmonella that causes typhoid fever because
it was originally designed for the purpose of
isolation of Salmonella that causes food
poisoning.
Formula
Determinations
Components
Yeast Extract
Peptone
Heart Extract Powder
Sodium Chloride
Mannitol
L-Lysine Hydrochloride
Sodium Thiosulfate
Ferric Ammonium Citrate
Brilliant Green
Crystal Violet
Agar
Inoculate the specimens directly or after the
enrichment, and incubate at 37°C for 18 –
24 hours.
Salmonella produces hydrogen sulfide and
forms black colonies, while the growths of
some strains of S. typhi and S. paratyphi A is
strongly inhibited, so the medium is
unsuitable for isolation of them.
Furthermore, the growth of bacteria except
Salmonella is strongly inhibited, and even
though they grow on the medium, their
colonies never turn black. Therefore
differentiation is easy.
2-9
In 49.0 g/L
5.0 g
10.0 g
2.0 g
4.0 g
3.0 g
5.0 g
4.0 g
1.0 g
0.0125 g
0.01 g
15.0 g
pH 6.8 ±
Back to the Index
05041-20100127 Koh SAI
LIM Medium “Nissui”
(Lysine Indole Motility Medium)
Code
Form
Package
Storage
Shelf Life
05104
Powder
60 g
Dry, RT
3 years
medium or only around the inoculum line, while
non-motile ones grow only along the inoculum
line.
Directions
Suspend 30.0 g of the dehydrated medium in
1,000 mL of distilled water, mix well and heat to
dissolve the medium. Distribute about 5 mL into
small test tubes. Sterilize by autoclaving at 121°C
for 15 minutes. Then cool immediately and use as
a semisolid butt.
Remarks
The medium has been found to be helpful in the
identification of Enterobacteriaceae, and it also
permits simultaneous examination of lysine
decarboxylation, indole production and motility of
bacteria. The combination with TSI Agar makes it
easy to perform the screening test of the
pathogenic bacteria, especially the differentiation
of Shigella, Salmonella and the like.
Determinations
Take the bacteria with a straight wire and stab into
about one third of the depth of the medium.
Incubate at 37°C for 18-24 hours.
●Determination of Lysine decarboxylation:
The positive reaction is indicated by violet color
(except light or faded violet) from the surface
down to the bottom. While in case of the negative
reaction, the deep or part changes into yellow and
only the surface remains violet.
●Determination of Indole production:
Add Kovac’s reagent into the tube. The color
change of the reagent into red constitutes the
positive indole reaction and no color change
constitutes the negative one.
●Determination of Motility:
Motile bacteria cause the turbidity of the whole
Formula
Components
Peptone
Yeast Extract
Dextrose
L-Lysine Hydrochloride
L-Tryptophan
Brom Cresol Purple
Agar
3-1
In 30.0 g/L
12.8 g
3.0 g
1.0 g
10.0 g
0.5 g
0.02 g
2.7 g
pH 6.8 ±
Back to the Index
05104-20100127 Koh SAI
SIM Medium “Nissui”
(Confirmation of Enteric bacteria)
Code
Form
Package
Storage
Shelf Life
05106
Powder
100 g
Dry, RT
3 years
Observation of hydrogen sulfide:
Positive bacteria change the color of
medium into black.
●Observation of motility:
Positive bacteria grow to get turbid whole
deep agar. Negative bacteria grow only
along with the stab portion.
Directions
●
Suspend 35.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute
about 5 mL into small test tubes, sterilize by
autoclaving at 121°C for 15 minutes and
cool immediately to a deep agar. Take
bacteria to be tested with a straight wire
and stab into the butt of the medium.
Incubate at 37°C for 24 hours.
Formula
Components
In 35.0 g/L
Remarks
Meat Extract
3.0 g
The medium, as a differentiation medium,
permits the tests of motility as well as
productions of indole and hydrogen sulfide
of the bacteria.
●Observation of indole production:
When an indole reagent is laid over, positive
bacteria change the color of reagent into
red, though negative one does not.
Peptone
28.0 g
Sodium Thiosulfate
0.025 g
Ferric Ammonium Citrate
1.0 g
Agar
3.0 g
pH 7.2 ±
Back to the Index
3-2
05106-20100128 Koh SAI
DNA Agar “Nissui”
(Deoxyribonucleic Acid Agar for detection of DNase)
Code
Form
Package
Storage
Shelf Life
05148
Powder
100 g
Dry, RT
3 years
determination of the DNase production.
The medium makes it possible to identify rapidly and
accurately the thermo-stable DNase production that is
useful for the detection of pathogenic Staphylococcus.
It can be used for the isolation of Serratia that is indicated
by the distinctive change of positive DNase production.
Directions
Suspend 42.1 g of the dehydrated medium in 1,000 mL of
distilled water, mix well and heat to dissolve the medium.
Sterilize by autoclaving at 121°C for 15 minutes.
Distribute about 20 mL of the medium into Petri dishes,
and dry out the surface of the medium before use.
Precautions for use
Determinations
The medium is not suitable for isolation of Staphylococcus.
By adding 1ml of cephalothin solution (100 mg/mL) to
100 mL of the medium, it can be used as the selective
medium for Serratia.
For the detection of DNase production, inoculate the
freshly cultured bacteria by streaking on the plate and
incubate at 37°C for 24 hours.
For the detection of thermo-stable DNase production, run
the test according to the simplified Menzie’s method.
For the isolation of Serratia, inoculate a small amount of
the specimen on the plate and incubate at 37°C for 20 24 hours.
Positive reaction of DNase production (+): The color of the
medium around the colonies turns red or reddish violet.
Negative reaction of DNase production (-): The color of the
medium around the colonies remains unchanged.
The detection of production of thermo-stable DNase
should also be interpreted in accordance with the abovementioned criteria.
Isolation of Serratia is indicated by the distinctive change
of positive DNase production.
Formula
Components
Peptone
Soya Peptone
Sodium Chloride
Deoxyribonucleic Acid
Toluidine Blue O
Agar
Remarks: (Features)
In 42.1 g/L
15.0 g
5.0 g
5.0 g
2.0 g
0.1 g
15.0 g
pH 7.2 ±
Back to the Index
As the medium contains toluidine blue O, there is no need
to add hydrochloric acid onto the colonies for the
3-3
05148-20100128 Koh SAI
SC Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05111
Powder
100 g
Dry, RT
3 years
Directions
Formula
Suspend 24.2 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute
about 3 mL of the medium into small test
tube and sterilize by autoclaving at 121°C
for 15 minutes. Solidify in a full slanted
position.
Take the bacteria to be tested with a
straight wire and smear on the slant.
Incubate at 37°C for 24 hours.
Components
In 24.2 g/L
Sodium Chloride
5.0 g
Magnesium Sulfate
0.2 g
Monoammonium
Phosphate
1.0 g
Dipotassium Phosphate
1.0 g
Sodium Citrate
2.0 g
Brom Thymol Blue
Remarks
Agar
The medium is used for an identification
test for Enteric bacteria providing a
confirmation of consumption of citrate.
Positive bacteria change the color of
medium into an intense blue with a
formation of colonies.
0.024 g
15.0 g
pH 6.8 ±
Back to the Index
3-4
05111-20100201 Koh SAI
VP-MR Medium “Nissui”
(Dextrose Phosphates Peptone Water, Voges-Proskauer Methyl Red Medium)
Code
Form
Package
Storage
Shelf Life
05107
Powder
100 g
Dry, RT
3 years
MR Test:
Add 2 to 3 drops of Methyl Red solution. A
red color develops if positive, while a yellow
color develops if negative.
Directions
Suspend 17.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute
about 2 mL of the medium into small test
tube and sterilize by autoclaving at 121°C
for 15 minutes. Incubate at 25-30°C for 2
days.
The medium is used for a confirmation test
of Enteric bacteria by VP and MR tests.
Formula
Components
Remarks
VP Test:
Add 1 ml of 6% α-Naphtol solution and 0.2
ml of 40% Potassium hydroxide, and shake
vigorously. A red color develops after several
minutes after it is left on slant position, if
positive. Negative is confirmed if no red
color developed even after one hour.
In 17.0 g/L
Peptone
7.0 g
Dextrose
5.0 g
Dipotassium Phosphate
5.0 g
pH 6.8 ±
Back to the Index
3-5
05107-20100201 Koh SAI
ONPG Disk “Nissui”
(For β-galactosidase test)
Code
Form
Package
Storage
Shelf Life
05182
Disk
50 Disks
Dark, Cool
1 year
galactosidase tsdt for examination of
properties of bacteria.
Directions
Put a disk in a small test tube and add 1.0
mL of sterilized distilled water. Inoculate the
organisms which have been cultured on the
agar medium, and mix well. Incubate at 37
°C for 18-24 hours and examine the
reaction. (An early decision in 1-2 hours is
possible.)
Formula
This disk contains ONPG (o-nitrophenyl-β-Dgalactopyranoside), peptone, and sodium
phosphate.
Back to the Index
Determination
Positive:
Decide as positive, if the solution turns
yellow. (The yellow color is due to onitrophenyl produced from ONPG by βgalactosidase.)
●Negative:
Decide as negative, if the solution remain
unchanged.
●
Remarks
The disk enables a simple and rapid β3-6
05182-201000204 Koh SAI
Cytochrome Oxidase Test Strip “Nissui”
Code
Form
Package
Storage
Shelf Life
05180
Piece
15 Pieces
Dark, Cool
-
Directions
Remarks
Put a piece into a Petri dish, add several
drops of distilled water to moisten the piece
wholly, and immediately smear organisms
to be tested (colony or slant culture) on the
strip using a platinum loop or a glass rod.
Note:
When a nichrome wire loop is used, use as
fresh a one as possible covering it with
absorbent cotton.
As the water should not contain iron ion,
use distilled water (or pure water passed
through ion-exchange resin).
This test strip was originally devised to
examine the presence of cytochrome
oxidase bacteria simply and accurately.
Accordingly, the strip is very suitable for the
examination of biological property for
Aeromonas, Pseudomonas, Vibrio, etc. and
for the differentiation of these bacteria from
Enterobacteriaceae.
Storage
Store in a dark and cool place. If not stored
properly, the strip will turn blue before use.
Such a strip should not be used.
Determinations
Cytochrome oxidase positive bacteria turn
the smeared area into deep blue within one
minute, but the area remains unchanged in
case of negative bacteria.
Formula
This product is a simplified Kovács oxidase
test.
Back to the Index
3-7
05180-20100204 Koh SAI
ID Test•EB-20 “Nissui”
(Identification Test Kit for Glucose fermentative Gram Negative bacilli)
Code
Package
06626
25 tests
06615
EB Plate
06616
EB Broth
06628
EB Reagent
Storage
RT
100 tests
1 year
Cool
solution and 40% Potassium Hydroxide Test solution (VP) into
certain wells.
After addition of specific reagents, compare the color
developed with the attached color chart and determine as
positive or negative results. Simultaneously, an oxidase test
shall be performed and record in the result form.
Features of ID Test•EB-20
Identification of bacteria is very complicated and there are so
many biochemical test need to be done. ID Test EB-20 is based on
the theory of the numerical classification method, which could
receive the combination with 20 kinds of biochemical reactions.
Only small amount of sample (0.1 mL) is needed for each test
item. Also the kit is stable for 1 year at room temperature.
Interpretations
Purpose of ID Test•EB-20
Refer “ID Test EB-20 ANALYTICAL PROFILE” for identification.
Formula
Identification of Glucose fermentative Gram Negative bacilli.
Directions for operation of the
kit
Streak samples onto blood agar or other isolation medium.
After incubation of the medium, confirm colonies will be
tested are Gram Negative bacilli and Glucose fermentative.
The test target bacteria shall be proliferated on enrichment
medium.
Fish colonies from enrichment medium for adjustment the
concentration (suspension) that corresponds to No.1
McFarland turbidity standard.
Inoculate 0.1 mL of above suspension into each well (20
wells). Layer 3-5 drops of sterilized liquid paraffin on 8 certain
wells and incubate (place the cover on the plate and incubate
at 37°C for 18 – 20 hours.
After incubation, add 1 drop of 10% Ferric Chloride Test
solution (PPA), Kovacs’ Reagent (IND), 6% α-naphthol Test
Shelf Life
Kit component (06626)
ID Test EB Plate
Sterilized Liquid Paraffin
Result Form
ID Test EB Broth
Color Chart
25 plates x 20 wells
20 mL x 1 vial
13 sheets x 2 sets
2.5 mL x 25 tubes
1 sheet
Kit component (06628)
10% Ferric Chloride Test
solution
Kovacs’ Reagent
6% α-naphthol Test solution
40% Potassium Hydroxide
Test solution
3-8
6 mL x 1 vial
6 mL x 1 vial
6 mL x 1 vial
6 mL x 1 vial
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06626-20100203 Koh SAI
ID Test•EB-9 “Nissui”
(Identification Test Kit for intestinal bacilli)
Code
Package
Storage
06673
50 tests
RT
100 tests
Cool
06671
EB Reagent
Features of ID Test•EB-9
1 year
Place the cover on the plate and incubate at 35°C-37°C for 18-24
hours.
In the following two items, after incubation, add the following
reagents and evaluate:
IND: Add 1 drop of Kovác's reagent.
VP: Add 1 drop of 6% α-Naphtol Reagent and also 1 drop of 40%
Potassium Hydroxide.
Compare the results with the appended color chart and determine
whether they are positive or negative.
Determine the species of bacteria by checking the code book
provided.
Identification of bacteria is very complicated and there are so
many biochemical test need to be done. ID Test EB-9 is based on
the theory of the numerical identification and microtest method,
and is suitable for identification of gram negative, oxidase
negative and glucose/lactose fermenting bacilli according to their
9 biochemical properties.
Two specimens will be simultaneously identified with one plate.
Major members of Enterrobacteriaceae will be identified with 18
hours incubation.
Package is so designed that many specimens will be
economically tested.
Formula
Kit component (06673)
EB-9 Plate
Sterilized Liquid Paraffin
Purpose of ID Test•EB-9
Identification of major intestinal bacilli.
Directions for operation of the kit
Streak the test specimen onto BTB Lactose Agar (Nissui Plate) or
MacConkey Agar (Nissui Plate) or DHL Agar (Nissui Plate), over
and culture for isolation of single colonies.
Confirm that the test bacteria on the siolation culture medium are
gram-negative and also oxidase-negative rods and that they are
glucose/lactose fermentative. These can be confirmed with
Cytochrome oxidase test and TSI agar. Exclude other bacteria.
Culture the test bacterium on nutrient agar.
●Preparation of Bacterial Suspension :
Collect the test bacteria multiplied by culture on a loop and
suspend them in one vial of ID Test. Adjust the concentration of
bacteria to a level No.1 of McFarland.
●Inoculation of Bacterial Suspension:
Draw up the prepared bacterial solution suing a sterilized capillary
piptte and inoculate into each well of the ID test Plate up to the
step (0.1 mL).
After inoculation, add 3-5 drops of sterile liquid paraffin on top of
the following 5 items (ARA, INO, LDC, ODC and URE) to form a
stratified layer in order to isolate them from the air.
Shelf Life
Result Form
EB-9 Broth
Kit component (06671)
Kovác's Reagent
6% α-naphthol Test
solution
40% Potassium Hydroxide
Test solution
2 test x 25 plates
20 mL x 2 tubes
2 tests x 26
sheets
1.5 mL x 50 tubes
6 mL x 1 tube
6 mL x 1 tube
6 mL x 1 tube
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3-9
06673-20100205 Koh SAI
Sterilized Oil Paraffin “Nissui”
(for ID Test)
Code
Form
Package
Storage
Shelf Life
06608
Liquid
50 mL
RT
-
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3-10
06608-20100205 Koh SAI
Quick ID GN “Nissui”
(Identification Test Kit for intestinal bacilli)
Code
Package
Storage
08720
25 tests
RT
100 tests
Cool
08721
Reagent
Shelf Life
1 year
from said Agar late.
Suspended a colony in the ID GN Broth device.
Recap and shake the device for several times to suspend well (the
conc. of McF.#0.5 is preferable) .
●Pour entire contents of broth tube into the center well of plate.
Put on the lid and incubate for 4 hours at 35°C-37°C.
Reading
After 4 hours incubation, remove the plates from the incubator.
Refer to the Color Chart for an interpretation (Positive or Negative)
of the reaction except for the item of ORO, PYR, IND and NIT.
These 4 items require the following additional procedure at room
temperature to judge:
PRO and PYR: Add a drop of Cinnamaldehyde reagent and allow it
to stand still for 5 minutes.
IND: Add a drop of Koác's reagent into the well of GC/I.
NIT: Add a drop of Nitrate reduction reagent into the well GL/N
and allow it to stand still for 5 minutes.
Features
●
The QUICK ID GN identification kit is a quick identification system
based upon the theories of the microtest and the numerical
classification methods. It is intended for the identification of
clinically significant aerobic gram-negative bacilli of human origin
with 4 hours according to the positive-negative color reactions for
14 biochemical tests includes 12 chromogenic substrate
reactions.
The bacteria suspension is prepared by mixing a small amount of
bacteria (one colony) .
The dispensing of bacteria suspension to the plate can be done
with one step.
Only 4 hours aerobic incubation at 35°C-37°C is required.
Wells on the plate are arranged by a group of three tests for
easier coding.
Directions for operation of the
kit
Formula
Kit component (08720)
Quick ID GN Plate
Quick ID GN Broth
Result Form
The identification is not for use directly with clinical specimens.
Use isolates from an agar plate such as said Agar plates.
The usefulness of the identification kit or any other diagnostic
procedure performed on clinical specimens is directly influenced
by the quality of the specimens themselves. It is strongly
recommended that laboratories employ methods discussed in the
Manual of Clinical Microbiology for specimen collection, transport
and placement isolation media.
Procedure
Remove plate from pouch and discard a desiccant.
Take a GN Broth device. Using aseptic technique, pick one well
isolated colony with the tip of a sterile applicator of the device
Kit component (08721)
Kovác's Reagent
Cinnamaldehyde Reagent
Nitrate Reduction
Reagent
3-11
25 plates
1 mL x 25 tubes
26 sheets
6 mL x 1 tube
6 mL x 2 tubes
6 mL x 1 tube
Back to the Index
08720-20100205 Koh SAI
Salt Polymyxin Broth “Nissui”
(For enrichment of Vibrio parahaemolyticus)
Code
Form
Package
Storage
Shelf Life
05215
Powder
100 g
Dry, RT
3 years
cultivation of the materials that seem to be
deficient in the number of Vibrio
parahaemolyticus.
Directions
Suspend 33.0 g of the dehydrated medium in
1,000 mL of distilled water, mix well and heat
to dissolve the medium. Distribute adequate
amounts into test tubes or flasks and then
sterilize by autoclaving at 121°C for 15
minutes. The amount of specimen is about
one tenth of the medium. Incubate at 37°C
overnight, then take a loopful of the culture
and inoculate on a plate of the selective
medium such as TCBS Agar.
Features
The preservation of the medium after
preparation is easy because the medium can
be autoclaved.
Since Polymyxin B, a selective antibiotic, is
unlikely to undergo its inactivation by
metabolities of bacteria, the medium can be
incubated overnight (up to 24 hours), and is
also convenient for routine examination.
Note
Formula
Incubation for 8 hours may be enough in an
urgent case.
Do not incubate for more than 24 hours to
prevent the growth of other bacteria.
Components
Peptone
Yeast Extract
Sodium Chloride
Polymyxin B
Remarks
The medium is suitable for the enrichment
culture of Vibrio parahaemolyticus from
foods, especially from fish and fish-products,
and for counting the number of bacteria (MPN
values). Furthermore, it is suitable for
In 33.0 g/L
10.0 g
3.0 g
20.0 g
250,000 unit
pH 7.4 ±
Back to the Index
4-1
05215-20100205 Koh SAI
Alkaline Peptone Water “Nissui”
(Enrichment of V. cholerae and V. parahaemolyticus)
Code
Form
Package
Storage
Shelf Life
05206
Powder
100 g
Dry, RT
3 years
Directions
Formula
Suspend 20.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute
about 10 mL of the medium into middle
size of test tube and sterilize by autoclaving
at 121°C for 15 minutes.
The medium is used for an enrichment
medium of Vibrio cholerae as well as Vibrio
parahaemolyticus. They grow mostly on the
surface of the medium.
Components
In 20.0 g/L
Peptone
10.0 g
Sodium Chloride
10.0 g
pH 8.8 ±
Back to the Index
Remarks
Since the medium has a weak selectivity, an
adequate incubation is at 37°C for 8 hours.
4-2
05206-20100208 Koh SAI
Vibrio Agar “Nissui”
(Isolation of V. cholerae and V. parahaemolyticus)
Code
Form
Package
Storage
Shelf Life
05201
Powder
300 g
Dry, RT
3 years
vibrios, while the addition of the indicators such
as cresol red and water blue makes the color
change of the colonies clear due to the
fermentation of saccharose.
Directions
Suspend 80.0 g of the dehydrated medium in
1,000 mL of distilled water, mix well and heat
to dissolve the medium. Distribute about 20
mL of the medium into Petri dishes without
sterilization by autoclaving.
Formula
Components
Yeast Extract
Peptone
Sucrose
Sodium Taurocholate
Sodium Lauryl Sulfate
Sodium Citrate
Sodium Thiosulfate
Disodium Phosphate
Ferric Citrate
Sodium Chloride
Agar
Water Blue
Cresol Red
Determinations
After drying the surface of the plate, inoculate a
larger amount of specimen than in case of
other selective media, and incubate at 37°C for
18 - 20 hours.
Saccharose fermentative bacteria such as
Vibrio cholerae and Vibrio alginolyticus form
blue colonies due to the color development of
water blue. Vibrio parahaemolyticus forms
slightly reddish and translucent colonies.
Remarks
The medium is a modification of DEC agar
medium and is useful for the isolation of both
V. cholerae and V. parahaemolyticus. The
composition of taurocholate and sodium lauryl
sulfate permits the growth of the pathogenic
In 80.0 g/L
5.0 g
5.0 g
12.5 g
5.0 g
0.2 g
8.0 g
8.5 g
7.5 g
3.0 g
10.0 g
15.0 g
0.2 g
0.02 g
pH 9.0 ±
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4-3
05201-20100208 Koh SAI
Polymyxin Mannose Tellurite Agar Base “Nissui”
(Isolation of El Tor cholera vibrio)
Code
Form
Package
Storage
Shelf Life
05208
Powder
240 g
Dry, RT
3 years
Alkaline Peptone Water at 35°C for 6 hours. Next, the
culture is inoculated in Monsur Peptone Water, and
incubate at 35°C for one night. Then the enrichment
culture is placed onto PMT Agar.
El Tor cholera vibrio grown on the medium can clearly
agglutinate the antiserum of V. cholerae.
When the medium is used for the clinical specimens such
as diarrheal feces, it is desirable to use it in combination
either with Vibrio Agar or with TCBS Agar simultaneously.
Directions
Suspend 60.0 g of the dehydrated medium in 1,000 mL of
distilled water, mix well and heat to dissolve the medium.
Cool the medium to about 60°C, add 1 mL of 0.1%
aqueous solution of potassium tellurite and mix well.
Distribute about 20 mL of the medium into Petri dishes.
No sterilization such as autoclaving is required. Dry the
surface of the medium before use.
After addition of 0.1% aqueous solution of potassium
tellurite, do not reheat.
Formula
Determinations
Components
Peptone
Meat Extract
Sodium Chloride
D-Mannose
Sodium Lauryl Sulfate
Polymyxin B
Brom Thymol Blue
Cresol Red
Agar
Inoculate the specimens directly or after enrichment
culture, and incubate at 37°C for 18 - 24 hours.
El Tor cholera vibrio forms yellow colonies with a brown
center due to the fermentation of mannose. Other vibrios
are mostly inhibited on the medium, but some organisms
may grow on it. However, their colonies are smaller than
those of El Tor cholera vibrio and have blue color. Thus,
the differentiation of El Tor cholera vibrio is easy. As a few
strains of NAG (non-agglutinable) vibrios form yellow
colonies, careful observation is needed.
Remarks
The medium is designed to detect El Tor cholera vibrio
from the specimens that are presumed to be
contaminated with a relatively small number of the
causative organisms. The first step of isolation for the
specimen such as river water is the pre-enrichment in
In 60.0 g/L
10.0 g
5.0 g
10.0 g
20.0 g
0.2 g
180,000 units
0.04 g
0.04 g
14.7 g
pH 8.4 ±
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4-4
05208-20100208 Koh SAI
TCBS Agar “Nissui”
(Isolation of V. cholerae and V. parahaemolyticus)
Code
Form
Package
Storage
Shelf Life
05204
Powder
300 g
Dry, RT
3 years
The procedure is very easy because sterilization is
not required.
Directions
Suspend 86.0 g of the dehydrated medium in
1,000 mL of distilled water, mix well and heat to
dissolve the medium. Distribute immediately
about 20 mL of the medium into Petri dishes. Do
not autoclave. Dry the surface of the medium
before use.
Note
It may be better to inoculate a larger amount of
specimen than in case of the other selective
media.
Formula
Determinations
Components
Yeast Extract
Peptone
Sucrose
Sodium Thiosulfate
Sodium Citrate
Sodium Cholate
Ferric Citrate
Sodium Chloride
Oxgall
Brom Thymol Blue
Thymol Blue
Agar
Inoculate the specimens directly or after
enrichment culture, and incubate at 37°C for 20 24 hours.
Saccharose fermentative bacteria such as Vibrio
cholerae and Vibrio alginolyticus form turbid,
yellow colonies, while saccharose nonfermentative bacteria such as Vibrio
parahaemolyticus form colonies with a greenish
blue center. Fecal streptococcus and Proteus may
sometimes grow, but their colonies are very small,
and the differentiation between them is easy.
Remarks
As the medium has high selectivity, the growths of
gram-positive bacteria and gram-negative, nonpathogenic bacteria in feces are inhibited.
4-5
In 86.0 g/L
5.0 g
10.0 g
17.0 g
10.0 g
10.0 g
3.0 g
1.0 g
10.0 g
5.0 g
0.04 g
0.04 g
15.0 g
pH 8.8 ±
Back to the Index
05204-20100208 Koh SAI
X-VP Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05135
Powder
300 g
Dry, RT
3 years
are inhibited.
Directions
Suspend 102.4 g of the dehydrated
medium in 1,000 mL of distilled water, mix
well and heat to dissolve the medium.
Distribute immediately about 20 mL of the
medium into Petri dishes. Do not autoclave.
Dry the surface of the medium before use.
Formula
Components
Peptone
Yeast Extract
Sucrose
Sodium Thiosulfate
Sodium Citrate
Sodium Chloride
Sodium Pyruvate
Bile Salt
Selective Agents
Colorimetric Substrate by
Enzyme
Agar
Determinations
Inoculate the specimens directly or after
enrichment culture, and incubate at 3537°C for 18-24 hours.
Vibrio parahaemolyticus form blue or blue
green colonies, Vibrio cholerae and Vibrio
vulnificus form purple colonies, Vibrio
alginolyticus form milky colonise.
In 102.4 g/L
Remarks
10.0 g
5.0 g
30.0 g
6.4 g
10.0 g
20.0 g
5.0 g
3.0 g
0.27 g
0.25 g
12.5 g
pH 8.8 ±
Back to the Index
As the medium has high selectivity, the
growths of bacteria except Vibrio in feces
4-6
05135-20100208 Koh SAI
NAC Agar “Nissui”
(For non-fermentative gram-negative rod)
Code
Form
Package
Storage
Shelf Life
05220
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 35.7 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. An
autoclaving is prohibited. Incubate at 35°C
for 24-48 hours.
Components
In 35.7 g/L
Peptone
Remarks
The medium is developed to isolate
Pseudomonas aeruginosa. The medium
enrich the production of fluorescent
pigment (pyocyanin and fluorescein) of
Pseudomonas aeruginosa, which makes a
differentiation easy.
Pseudomonas aeruginosa develops a colony
with yellow-greenish fluorescent on the
medium. Both gram positive and gram
negative bacteria other than Pseudomonas
aeruginosa are almost all inhibited on the
medium.
20.0 g
Dipotassium Phosphate
0.3 g
Magnesium Sulfate
0.2 g
Cetrimide
0.2 g
Nalidixic acid
Agar
0.015 g
15.0 g
pH 7.4 ±
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5-1
05220-20100208 Koh SAI
Cetrimide Agar “Nissui”
(For non-fermentative gram-negative rod)
Code
Form
Package
Storage
Shelf Life
05221
Powder
300 g
Dry, RT
3 years
negative bacteria other than Pseudomonas
aeruginosa are almost all inhibited on the
medium.
Directions
Suspend 46.9 g of the dehydrated medium
in 1,000 mL of distilled water, add 10 mL of
glycerin, mix well and heat to dissolve the
medium. Sterilize by autoclaving at 121°C
for 15 minutes. Distribute about 20 mL of
the medium into Petri dishes.Dry the
surface of the medium before use. Incubate
at 30-35°C for 18-72 hours.
Formula
Components
In 46.9 g/L
Peptone
20.0 g
Magnesium Chloride
Remarks
Potassium Sulfate
The medium is developed by Brown and
Lowbury to isolate Pseudomonas
aeruginosa. The medium enrich the
production of fluorescent pigment
(pyocyanin and fluorescein) of
Pseudomonas aeruginosa, which makes a
differentiation easy.
Pseudomonas aeruginosa develops a colony
with yellow-greenish fluorescent on the
medium. Both gram positive and gram
Cetrimide
3.0 g
10.0 g
0.3 g
Agar
13.6 g
pH 7.2 ± 0.2
Back to the Index
5-2
05221-20100208 Koh SAI
ID Test•NF-18 “Nissui”
(Identification Test Kit for Glucose Non-fermentative Gram Negative bacilli)
Code
Package
06629
25 tests
06617
NF Plate
06618
NF Broth
06631
NF Reagent
Storage
RT
100 tests
Shelf Life
1 year
Cool
Detection Test Solution (I and II) (NIT) into certain wells.
After addition of specific reagents, compare the color developed
with the attached color chart and determine as positive or
negative results. Simultaneously, an oxidase test shall be
performed and record in the result form.
Features of ID Test NF-18
Identification of bacteria is very complicated and there are so
many biochemical test need to be done. ID Test NF-18 is based on
the theory of the numerical classification method, which could
receive the combination with 18 kinds of biochemical reactions.
Only small amount of sample (0.1 mL) is needed for each test
item. Also the kit is stable for 1 year at room temperature.
Interpretations
Refer “ID Test NF-18 ANALYTICAL PROFILE” for identification.
Purpose of ID Test NF-18
Formula
Identification of Glucose Non-fermentative Gram Negative bacilli
Kit component (06629)
ID Test NF Plate
Sterilized Liquid Paraffin
Result Form
ID Test NF Broth
Color Chart
Directions for operation of the
kit
Streak samples onto blood agar or other isolation medium. After
incubation of the medium, confirm colonies will be tested are
Gram Negative bacilli and Glucose fermentative.
The test target bacteria shall be proliferated on enrichment
medium.
Fish colonies from enrichment medium for adjustment the
concentration (suspension) that corresponds to No.0.5 McFarland
turbidity standard.
Inoculate 0.1 mL of above suspension into each well (18 wells).
Layer 3-5 drops of sterilized liquid paraffin on 9 certain wells and
incubate (place the cover on the plate and incubate at 30 or 37°C
for 22 – 24 hours.
After incubation, add 1 drop of Kovacs’ Reagent (IND), Nitrate
25 plates x 18 wells
20 mL x 2 vial
13 sheets x 2 sets
2.5 mL x 25 tubes
1 sheet
Kit component (06631)
Nitrite Detection Test
Solution I
Kovács’ Reagent
Nitrite Detection Test
Solution II
Zinc Powder
5-3
6 mL x 1 vial
6 mL x 1 vial
6 mL x 1 vial
6 mL x 1 vial
Back to the Index
06629-20100208 Koh SAI
Staphylococcus Medium No. 110 “Nissui”
(Selective isolation medium for Staphylococci)
Code
Form
Package
Storage
Shelf Life
05234
Granule
300 g
Dry, RT
3 years
inhibited to some extent.
Pathogenic staphylococci generally form
yellowish or lemon-colored colonies on the
medium, while non-pathogenic strains form
white ones.
The ability for producing pigment, utilizing
mannitol and liquefying gelatin can be
directly examined on the medium.
Directions
Suspend 150.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes.
Distribute about 20 mL of the medium into
Petri dishes. Dry the surface of the plate
before use.
Formula
Determinations
Components
Peptone
Yeast Extract
Gelatin
Mannitol
Sodium Chloride
Lactose
Dipotassium Phosphate
Agar
Incubate at 30°C for 48 hours or 37°C for 43
hours.
Pathogenic staphylococci form yellowish or
lemon-colored colonies on the medium and
have abilities to utilize mannitol, to liquefy
gelatin (positive to Stone reaction), to
coagulate plasma and to hemolyze rabbit
blood.
Remarks
Most of bacteria except staphylococci are
inhibited on the medium, and some of the
non-pathogenic staphylococci are also
In 150.0 g/L
10.0 g
2.5 g
30.0 g
10.0 g
75.0 g
2.0 g
5.0 g
15.0 g
pH 7.5 ±
Back to the Index
6-1
05234-20100208 Koh SAI
Mannitol Salt Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05236
Granule
300 g
Dry, RT
3 years
pathogenic staphylococci is performed by
checking the ability to utilize mannitol.
For differential medium of coagulase positive
staphylococci, add egg yolk solution to the
medium. To prepare the 10% salt egg yolk
agar medium, suspend 111.0 g of the
dehydrated medium in 900 ml of distilled
water, sterilize by autoclaving and cool down to
50°C, to which add 100 ml of egg yolk solution
(mixture of about 20 g of egg yolk and 80 ml
sterilized physiological saline solution).
Directions
Suspend 111.0 g of the dehydrated medium in
1,000 mL of distilled water, mix well and heat
to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes. Distribute
about 20 mL of the medium into Petri dishes.
Dry the surface of the plate before use.
Determinations
Incubate at 30-35°C for 18-72 hours.
When staphylococci, which utilize mannitol,
grow on the medium, they form yellow colonies
and turn the medium around the colonies
yellow, and Staphylococcus aureus can easily
be differentiated from non–pathogenic
staphylococci and other bacteria that do not
utilize mannitol and form red colonies.
Formula
Components
Peptone
Meat Extract
Sodium Chloride
Mannitol
Phenol Red
Agar
Remarks
As the medium contains salt of high
concentration, non-halophilic bacteria are
inhibited to grow, and staphylococci are
selectively isolated. The screening test of
In 111.0 g/L
10.1 g
6.5 g
71.3 g
9.2 g
0.023 g
13.8 g
pH 7.4 ± 0.2
6-2
Back to the Index
05236-20100209 Koh SAI
Phenylethyl Alcohol Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05246
Powder
100 g
Dry, RT
1 year
inhibited on the medium. Even if they grow
on it, they form only small colonies. Blood
agar prepared by adding blood to the
medium in a ration of 5% is especially
useful as the selective medium for the types
of streptococci and anaerobic bacteria
including obligate anaerobes and some of
clostridia.
Directions
Suspend 42.5 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes.
Distribute 20 mL of the medium into Petri
dishes.
Use the medium within a day of its
preparation, and do not preserve it. The
medium, once solidified, should not be
dissolved again.
For isolation of anaerobic bacteria, the
incubation time should usually be 48 hours
and 72 hours at the longest.
Formula
Components
Peptone
Soya Peptone
Sodium Chloride
Phenylethyl Alcohol
Agar
Remarks
The medium can be used for isolation of
gram-positive cocci such as streptococci
and staphylococci from the materials in
which gram-negative bacteria such as
Escherichia coli and Proteus coexist.
The gram-negative bacteria are mainly
In 42.5 g/L
15.0 g
5.0 g
5.0 g
2.5 g
15.0 g
pH 7.3 ±
Back to the Index
6-3
05246-20100209 Koh SAI
Salt Egg Yolk Agar Base “Nissui”
Code
Form
Package
Storage
Shelf Life
05238
Granule
300 g
Dry, RT
3 years
medium in ring shapes from the lower part of
the colonies. In 18 – 24 hours of incubation
pearly-lustrous rings like those made by oil
drops falling upon the water surface are formed
around the colonies on the medium. Therefore,
even the pearly-lustrous rings alone can serve
as a means of differentiation of coagulasepositive staphylococci. Colonies of coagulasenegative staphylococci are rather opaque and
do not form pearly-lustrous rings or opacify the
medium.
Directions
Suspend 115.0 g of the dehydrated medium in
1,000 mL of distilled water, mix well and heat
to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes. Cool
down to approximately 50-60°C. Add an egg
yolk suspension in a ratio of 10% with thorough
mixing. Distribute about 20 mL of the medium
into Petri dishes. Dry the surface of the plate
before use.
For preparation of an egg yolk suspension,
aseptically add one egg yolk to 30 ml of 10%
sterilized sodium chloride solution, and make
homogeneous egg yolk suspension by thorough
mixing. It is advisable to take the egg yolk from
an egg that weights more than 50 g.
Formula
Components
Heart Extract Powder
Peptone
Sodium Chloride
Lithium Chloride
Mannitol
Agar
Determinations
Inoculate the specimens on the plate and
incubate at 37°C for 24–40 hours.
Coagulase-positive staphylococci form clear
yellow or lemon-colored colonies. In 20 hours
of incubation in a short-time case or in 40
hours at the longest, the colonies opacify the
In 115.0 g/L
5.0 g
10.0 g
70.0 g
5.0 g
10.0 g
15.0 g
pH 7.3 ±
Back to the Index
6-4
05238-20100209 Koh SAI
N-ID Test•SP-18 “Nissui”
(Identification Test Kit for Staphylococci)
Code
Package
06637
25 tests
06613
SP Plate
06614
SP Broth
06638
SP Reagent
Storage
RT
100 tests
Shelf Life
1 year
Cool
naphthol and 40% Potassium Hydroxide Test solution (VP) into
certain wells.
After addition of specific reagents, compare the color developed
with the attached color chart and determine as positive or
negative results.
Features of N-ID Test SP-18
Identification of bacteria is very complicated and there are so
many biochemical test need to be done. N-ID Test SP-18 is based
on the theory of the numerical classification method, which could
receive the combination with 18 kinds of biochemical reactions.
Only small amount of sample (0.1 mL) is needed for each test
item. Also the kit is stable for 1 year at room temperature.
Interpretations
Refer “N-ID Test SP-18 ANALYTICAL PROFILE” for identification.
Purpose of ID Test NF-18
Formula
For identification of various species of Staphylococci.
Kit component (06637)
N-ID Test SP Plate
Sterilized Liquid Paraffin
Result Form
N-ID Test SP Broth
Color Chart
Directions for operation of the
kit
Streak samples onto blood agar or other isolation medium. After
incubation of the medium, confirm colonies will be tested are
Gram Negative bacilli and Glucose fermentative.
The test target bacteria shall be proliferated on enrichment
medium.
Fish colonies from enrichment medium for adjustment the
concentration (suspension) that corresponds to No.3 McFarland
turbidity standard.
Inoculate 0.1 mL of above suspension into each well (18 wells).
Layer 3-5 drops of sterilized liquid paraffin on 2 certain wells and
incubate (place the cover on the plate and incubate at 30 or 37°C
for 22 – 24 hours. (Wells 1 and 2 are empty wells and inoculation
of bacterial sample solution is not necessary.
After incubation, add 1 drop of Kovacs’ Reagent (IND), 6% α-
25 plates x 18 wells
20 mL x 1 vial
13 sheets x 2 sets
2.5 mL x 25 tubes
1 sheet
Kit component (06638)
6% α-naphthol Test solution
40% Potassium Hydroxide
Test solution
Nitrite Detection Test
Solution I
Nitrite Detection Test
Solution II
6-5
6 mL x 1 vial
6 mL x 1 vial
6 mL x 1 vial
6 mL x 1 vial
Back to the Index
06637-20100209 Koh SAI
ID Test• HN-20 Rapid “Nissui”
(For Identification of Haemophilus and Neisseria)
Code
Package
Storage
06635
25 tests
RT
100 tests
Cool
06640
Reagent
Features
Shelf Life
1 year
Formula
ID TEST•NH-20 Rapid “Nissui” has been
developed based on the theory of the
microtest method and numerical
classification. With this method,
Haemophilus and Neisseria are identified
rapidly in 4-hour incubation from positive or
negative patterns of 20 different
biochemical properties.
In this system, results can be obtained
which those of conventional methods with
respect to the gram-negative bacteria of
Neisseria, Pasteurella, including
Haemophilus, or the bacteria of extremely
weak growth such as Gardnerella or
Eikenella.
Moreover, identification includes
simultaneous confirmation of β-lactamase
production by the β-lactamase test.
Kit component (06635)
ID Test HN Plate
ID Test HN Broth
Result Form
β-lactamase Detection
Reagent
Sterilized Liquid Paraffin
25 plates x 20 wells
2.5 mL x 25 tubes20
mL x 1 vial
13 sheets x 2 sets
3 mL x 1 vial
20 mL x 1 vial
Kit component (06640)
Kovács’ Reagent
Nitrite Detection Test
Solution I
Nitrite Detection Test
Solution II
Zinc Powder
6 mL x 1 vial
6 mL x 1 vial
6 mL x 1 vial
2.5 g x 1 vial
Back to the Index
7-1
06640-20100209 Koh SAI
GAM Agar “Nissui”
(Gifu Anaerobic Medium Agar, For common culture and susceptibility test)
Code
Form
Package
Storage
Shelf Life
05420
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 74.0 g of the dehydrated medium
in 1,000 mL of distilled water, and heat to
dissolve the medium. Sterilize by
autoclaving at 115°C for 15 minutes.
Distribute 20 mL amounts of the medium
into Petri dishes.
Prepared medium should be used in a day
of preparation, or keep in anaerobic
condition.
Components
Peptone
Soya Peptone
Proteose Peptone
Digested Serum
Yeast Extract
Meat Extract
Liver Extract
Dextrose
Potassium Dihydrogen
Phosphate
Sodium Chloride
Soluble Starch
L-Cysteine Hydrochloride
Sodium Thioglycollate
Agar
Remark
The medium is developed by Medical School
of Gifu University, Japan to isolate and
culture anaerobic bacteria from clinical
specimens. The medium is also used for
susceptibility tests other than sulfa drugs.
In 74.0 g/L
10.0 g
3.0 g
10.0 g
13.5 g
5.0 g
2.2 g
1.2 g
3.0 g
2.5 g
3.0 g
5.0 g
0.3 g
0.3 g
15.0 g
pH 7.1 ±
Back to the Index
8-1
05420-20100210 Koh SAI
GAM Agar, Modified “Nissui”
(Gifu Anaerobic Medium Agar, Modified, For common culture and susceptibility test)
Code
Form
Package
Storage
Shelf Life
05426
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 56.7 g of the dehydrated medium
in 1,000 mL of distilled water, and heat to
dissolve the medium. Sterilize by
autoclaving at 115°C for 15 minutes.
Distribute 20 mL amounts of the medium
into Petri dishes.
Prepared medium should be used in a day
of preparation, or keep in anaerobic
condition.
Components
Peptone
Soya Peptone
Proteose Peptone
Digested Serum
Yeast Extract
Meat Extract
Liver Extract
Dextrose
Soluble Starch
L-Tryptophan
L-Cysteine Hydrochloride
Sodium Thioglycollate
L-Arginine
Vitamin K1
Hemin
Remark
The medium is a modified medium of GAM
Agar that is developed by Medical School of
Gifu University, Japan to isolate and culture
anaerobic bacteria from clinical specimens.
The medium is also used for susceptibility
tests other than sulfa drugs.
Color of the medium is more pale and
better transparency.
Potassium Dihydrogen Phosphate
Sodium Chloride
Agar
8-2
In 56.7 g/L
5.0 g
3.0 g
5.0 g
10.0 g
2.5 g
2.2 g
1.2 g
0.5 g
5.0 g
0.2 g
0.3 g
0.3 g
1.0 g
5 mg
10 mg
2.5 g
3.0 g
15.0 g
pH 7.3 ±
Back to the Index
05426-20100210 Koh SAI
GAM Broth, Modified “Nissui”
(Gifu Anaerobic Medium Broth, Modified, For common culture and susceptibility test)
Code
Form
Package
Storage
Shelf Life
05433
Powder
100 g
Dry, RT
3 years
Directions
Formula
Components
Peptone
Soya Peptone
Proteose Peptone
Digested Serum
Yeast Extract
Meat Extract
Liver Extract
Dextrose
Soluble Starch
L-Tryptophan
L-Cysteine Hydrochloride
Sodium Thioglycollate
L-Arginine
Vitamin K1
Hemin
Potassium Dihydrogen
Phosphate
Sodium Chloride
Suspend 41.7 g of the dehydrated medium
in 1,000 mL of distilled water, and heat to
dissolve the medium. Distribute the
medium into appropriate containers.
Sterilize by autoclaving at 115°C for 15
minutes, and cool down quickly (Do not
shake the medium!).
Remark
The composition of the medium is exactly
same as GAM Agar, Modified excluding
agar. The medium is liquid and used for
isolation and cultivation of anaerobic
bacteria from clinical specimens. The
medium is also used for susceptibility tests
other than sulfa drugs, in particular for the
micro liquid dilution method of anaerobic
bacteria.
8-3
In 41.7 g/L
5.0 g
3.0 g
5.0 g
10.0 g
2.5 g
2.2 g
1.2 g
0.5 g
5.0 g
0.2 g
0.3 g
0.3 g
1.0 g
5 mg
10 mg
2.5 g
3.0 g
pH 7.3 ±
Back to the Index
05433-20100210 Koh SAI
GAM Broth “Nissui”
(Gifu Anaerobic Medium Broth, For common culture and susceptibility test)
Code
Form
Package
Storage
Shelf Life
05422
Powder
300 g
Dry, RT
3 years
pneumococci and meningococci.
The medium is also suitable for blood culture.
Directions
Suspend 59.0 g of the dehydrated medium in
1,000 mL of distilled water, and heat to
dissolve the medium. Distribute the medium
into test tubes or flasks and sterilize by
autoclaving at 115°C for 15 minutes.
For cultivation of anaerobic bacteria, cool the
medium immediately after the sterilization in
running water without shaking or moving,
inoculate the organisms at once with a
sterilized capillary or a Pasteur pipette, and
incubate under the anaerobic condition.
Formula
Components
Peptone
Soya Peptone
Proteose Peptone
Digested Serum
Yeast Extract
Meat Extract
Liver Extract
Dextrose
Potassium Dihydrogen
Phosphate
Sodium Chloride
Soluble Starch
L-Cysteine Hydrochloride
Sodium Thioglycollate
Remark
This is a liquid medium for anaerobic
bacteria. Agar is removed from GAM Agar
“Nissui”. As the medium contains the
digested serum that has hemin, it is
successfully used for cultivation of anaerobic
bacteria. It has a broad application to other
microorganisms such as streptococci,
8-4
In 59.0 g/L
10.0 g
3.0 g
10.0 g
13.5 g
5.0 g
2.2 g
1.2 g
3.0 g
2.5 g
3.0 g
5.0 g
0.3 g
0.3 g
pH 7.1 ±
Back to the Index
05422-20100210 Koh SAI
GAM Semisolid without Dextrose “Nissui”
(Gifu Anaerobic Medium Semisolid without Dextrose, For identification and strain preservation )
Code
Form
Package
Storage
Shelf Life
05460
Powder
100 g
Dry, RT
3 years
Directions
Formula
Suspend 52.5 g of the dehydrated medium
in 1,000 mL of distilled water, and heat to
dissolve the medium. Distribute the
medium into test tubes (1.5 × 15 cm or 0.9
× 15 cm is recommended) up to a level
slightly higher than the middle (about 16
mL). Sterilize by autoclaving at 115°C for
15 minutes, and immediately cool down to
a stab culture agar.
Store at room temperature in a dark place.
Do not store in refrigerator.
Components
Peptone
Soya Peptone
Proteose Peptone
Digested Serum
Yeast Extract
Meat Extract
Liver Extract
Potassium Dihydrogen
Phosphate
Sodium Chloride
L-Cysteine Hydrochloride
Sodium Thioglycollate
Agar
Remark
The medium is devised as a basal medium
for testing the carbohydrate utilization by
anaerobes. It is suitable for tests of various
biochemical properties, motility and
preservation of anaerobes.
In 52.5 g/L
10.0 g
3.0 g
10.0 g
13.5 g
5.0 g
2.2 g
1.2 g
2.5 g
3.0 g
0.3 g
0.3 g
1.5 g
pH 7.1 ±
Back to the Index
8-5
05460-20100212 Koh SAI
GAM Semisolid “Nissui”
(Gifu Anaerobic Medium, For cultivation of clinical specimens and preservation of organisms)
Code
Form
Package
Storage
Shelf Life
05424
Powder
100 g
Dry, RT
3 years
Directions
Formula
Suspend 60.5 g of the dehydrated medium in 1,000 mL
of distilled water, and heat to dissolve the medium.
Distribute the medium into test tubes .
Sterilize by autoclaving at 115°C for 15 minutes. Store
them at room temperature in a dark place. Do not
store in the refrigerator.
Components
Peptone
Soya Peptone
Proteose Peptone
Digested Serum
Yeast Extract
Meat Extract
Liver Extract
Determinations
Obligate anaerobes dislike the part 5 – 10 mm below
the surface of the medium and grow in the lower part.
Obligate aerobes grow in the upper part and facultative
anaerobes grow throughout the medium. However,
judgement is difficult in case of gas-producing bacteria.
In 60.5 g/L
10.0 g
3.0 g
10.0 g
13.5 g
5.0 g
2.2 g
1.2 g
Dextrose
Remark
3.0 g
Potassium Dihydrogen
Phosphate
Sodium Chloride
Soluble Starch
L-Cysteine Hydrochloride
Sodium Thioglycollate
Agar
The medium is a semisolid medium devised by
reducing the agar content of GAM Agar. As the
medium permits rapid growth of aerobes as well as
anaerobes, it is suitable for culture of blood, puncture
fluid and other various materials, and for cultivation
and preservation of anaerobes.
The major character of the medium is to allow active
proliferation of anaerobes in the air without using a
special apparatus for the anaerobic condition.
2.5 g
3.0 g
5.0 g
0.3 g
0.3 g
1.5 g
pH 7.1 ±
Back to the Index
8-6
05424-20100212 Koh SAI
CW Agar Base without Kanamycin “Nissui”
(Clostridium welchii Agar base without Kanamycin, For examination of heated materials)
Code
Form
Package
Storage
Shelf Life
05404
Powder
100 g
Dry, RT
3 years
On the medium added with defibrinated
blood instead of egg yolk, Clostridium
welchii (perfringens) forms colonies of the
same type with a glistering surface and a
hemolytic ring. When the colonies are
exposed to the air in an incubator, they will
turn green.
Directions
Suspend 60.0 g of the dehydrated medium
in 900 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes.
Maintain the sterilized medium at about
50°C, add 100 mL of the egg yolk saline
suspension or defibrinated blood. Mix well
and distribute about 20 ml into Petri dishes.
To prepare a 100 mL of egg yolk solution,
mix about 20 g of egg yolk with 80 mL
sterilized physiological saline solution.
Formula
Components
Heart Extract
Proteose Peptone
Peptone
Sodium Chloride
Lactose
Phenol Red
Agar
Determinations
After drying the surface of the plate, smear
the heated material, and incubate for 15 –
20 hours under anaerobic condition.
Clostridium welchii (perfringens) forms
yellowish white, round, convex colonies with
a glistering surface and surrounded by a
pearly ray zone (opacity of the medium).
In 60.0 g/L
5.0 g
10.0 g
10.0 g
5.0 g
10.0 g
0.05 g
20.0 g
pH 7.2 ±
Back to the Index
8-7
05404-20100212 Koh SAI
CW Agar Base without Kanamycin “Nissui”
(Clostridium welchii Agar base with Kanamycin, For examination of unheated materials)
Code
Form
Package
Storage
Shelf Life
05405
Powder
100 g
Dry, RT
3 years
On the medium added with defibrinated blood
instead of egg yolk, Clostridium welchii
(perfringens) forms colonies of the same type
with a glistering surface and a hemolytic ring.
When the colonies are exposed to the air in an
incubator, they will turn green.
Directions
Suspend 60.0 g of the dehydrated medium in
900 mL of distilled water, mix well and heat to
dissolve the medium. The heating should be
done for less than 90 minutes, Maintain the
medium at about 50°C, add 100 mL of the egg
yolk saline suspension. Mix well and distribute
about 20 mL into Petri dishes. When a
C.perfringens Differentiation Strip is used,
place the stick into the center of medium
before it solidifies.
To prepare a 100 mL of egg yolk solution, mix
about 20 g of egg yolk with 80 mL sterilized
physiological saline solution.
Formula
Components
Heart Extract
Proteose Peptone
Peptone
Sodium Chloride
Lactose
Phenol Red
Kanamycin Sulfate
Agar
Determinations
After drying the surface of the plate, smear the
unheated material, and incubate for 15 – 20
hours under anaerobic condition. Clostridium
welchii (perfringens) forms yellowish white,
round, convex colonies with a glistering surface
and surrounded by a pearly ray zone (opacity of
the medium).
In 60.0 g/L
5.0 g
10.0 g
10.0 g
5.0 g
10.0 g
0.05 g
0.2 g
20.0 g
pH 7.2 ±
Back to the Index
8-8
05405-20100212 Koh SAI
Bacteroides Agar “Nissui”
(For differentiation and isolation of Bacteroides)
Code
Form
Package
Storage
Shelf Life
05440
Powder
100 g
Dry, RT
3 years
Directions
medium also permits the selective growth of Bacteroides from
the clinical specimens.
Suspend 74.0 g of the dehydrated medium in 1,000 mL of
distilled water, mix well and heat to dissolve the medium.
Sterilize by autoclaving at 121°C for 15 minutes, and distribute
the medium into Petri dishes. Dry the surface sufficiently before
use. It is desirable to use the plate within 3 – 5 hours after
preparation.
Formula
Components
Peptone
Soya Peptone
Digested Serum
Liver Extract
Meat Extract
Yeast Extract
Hemin
Dextrose
Sodium Chloride
Determinations
Differentiation: The medium is able to identify Bacteroides from
the organisms that are isolated from clinical specimens and
confirmed to be asporogenic gram-negative bacilli of obligate
anaerobes. Inoculate the bacilli into GAM Semisolid “Nissui” and
incubate for 24 – 48 hours. Take the organisms, streak the
surface of the plate, and incubate at 37°C for 24 – 48 hours
under an anaerobic condition. The organisms are identified as
Bacteroides, when they grow well on this plate.
Selective isolation: When clinical specimens are considered to be
lightly contaminated with other organisms, Bacteroides can be
isolated selectively by smearing them directly. (Fusobacterium
does not grow on this medium, but some aerobic cocci may grow
on it).
For the quantitative culture, dilute a sample with the following
solution (prepared by 1/15 M phosphate-buffered solution, pH
7.2, containing 1 g of polysorbate 80.1 g of L-cysteine
hydrochloride and 1 g of agar per liter by autoclaving at 110°C for
15 minutes).
Remarks
The medium was devised for the differentiation of Bacteroides.
Colistin, neomycin and brilliant green have no influence on the
growth of Bacteroides but inhibit Fusobacterium and other
bacteria. Asporogenic gram-negative bacilli taken from the
clinical specimens are mainly Bacteroides or Fusobacterium. The
Potassium Dihydrogen Phosphate
Soluble Starch
L-Cysteine Hydrochloride
Sodium Thioglycollate
Colistin
Neomycin
Brilliant Green
Agar
8-9
In 74.0 g/L
20.0 g
1.5 g
6.75 g
0.6 g
6.15 g
10.0 g
0.003 g
3.0 g
3.0 g
2.5 g
5.0 g
0.3 g
0.3 g
1,000,000 units
0.2 g
0.001 g
14.7 g
pH 7.1 ±
Back to the Index
05440-20100212 Koh SAI
FM Agar, Modified “Nissui”
(For differentiation and isolation of Fusobacterium)
Code
Form
Package
Storage
Shelf Life
05441
Powder
100 g
Dry, RT
3 years
Directions
Formula
Suspend 74.0 g of the dehydrated medium in
1,000 mL of distilled water, and heat to dissolve
the medium. Do not sterilize by autoclaving.
Distribute the medium into Petri dishes.
Components
Peptone
Soya Peptone
Digested Serum
Liver Extract
Meat Extract
Yeast Extract
Dextrose
Potassium Dihydrogen
Phosphate
Sodium Chloride
Soluble Starch
L-Cysteine Hydrochloride
Sodium Thioglycollate
Neomycin
Crystal Violet
Agar
Determination
The medium is designed to differentiate
Fusobacterium. Neomycin and crystal violet have
no influence on the growth of Fusobacterium, but
inhibit Bacteroides and other bacteria.
Since it is well known fact that almost all gramnegative rods, which do not form spore, are
Fusobacterium and Bacteroides., the medium is
used for the isolation of Fusobacterium. Among of
non-spore forming, gram-negative anaerobic rods,
the bacteria grown on the medium is to be
identified as Fusobacterium.
Remarks
To prepare the medium, heating should be
minimum, and use the medium in 3 – 5 hours
after preparation.
Incubate the medium avoiding excess disclosure
to the air.
In 74.0 g/L
20.0 g
1.5 g
6.75 g
0.6 g
6.15 g
10.0 g
3.0 g
2.5 g
3.0 g
5.0 g
0.3 g
0.3 g
0.2 g
0.01 g
14.7 g
pH 7.1 ±
Back to the Index
8-10
05441-20100212 Koh SAI
GAM Agar with Gentamicin “Nissui”
(For selective isolation of anaerobes)
Code
Form
Package
Storage
Shelf Life
05450
Powder
300 g
Dry, RT
3 years
The selectivity of the medium is based on the fact
that anaerobes are resistant to gentamicin but
aerobes are not.
Directions
Suspend 74.0 g of the dehydrated medium in 1,000
mL of distilled water, and heat to dissolve the
medium. Sterilize by autoclaving at 115°C for 15
minutes. Distribute about 20 mL of medium into
Petri dishes.
Formula
Components
Peptone
Soya Peptone
Proteose Peptone
Digested Serum
Yeast Extract
Meat Extract
Liver Extract
Dextrose
Determination
Smear the specimens and incubate under the
anaerobic condition.
Most of organisms that grow anaerobically on this
medium may be considered as anaerobes. However,
when the specimens are contaminated with
gentamicin-resistant facultative anaerobes, they
may grow on this medium. Thus, it is necessary to
confirm if the anaerobes do not grow on this
medium under the aerobic condition.
Potassium Dihydrogen Phosphate
Sodium Chloride
Soluble Starch
L-Cysteine Hydrochloride
Sodium Thioglycollate
Gentamicin
Agar
Remarks
The medium was designed for the separation of
anaerobes from the specimens that are suspected
to be contaminated with aerobes. On the medium,
almost all anaerobes including Bacteroides,
Fusobacterium, Actinomyces, Peptococcus,
Peptostreptococcus, Veillonella and Clostridium
grow well, while many aerobes do not.
8-11
In 74.0 g/L
10.0 g
3.0 g
10.0 g
13.5 g
5.0 g
2.2 g
1.2 g
3.0 g
2.5 g
3.0 g
5.0 g
0.3 g
0.3 g
25 mg
15.0 g
pH 7.1 ±
Back to the Index
05450-20100212 Koh SAI
BL Agar “Nissui”
(For isolation of anaerobes and differentiation of Bifidobacterium)
Code
Form
Package
Storage
Shelf Life
05430
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 58.0 g of the dehydrated medium in
1,000 mL of distilled water, mix well and heat
to dissolve the medium. Sterilized by
autoclaving at 115°C for 20 minutes. After
cooling to 50°C, add defibrinated horse blood
in the ratio of 5 percent and mix well.
Distribute into Petri dishes, and solidify. Dry
the surface before use.
Components
Meat Extract
Proteose Peptone
Peptone
Soya Peptone
Yeast Extract
Liver Extract
Dextrose
Soluble Starch
Determinations
Bifidobacterium generally forms smooth and
brown or dark brown colonies. Some enteric
bacteria form red colonies. For the details,
see the “World of Intestinal Bacteria” written
by Dr. Tomotari Mitsuoka.
Monopotassium Phosphate
Potassium Dihydrogen Phosphate
Magnesium Sulfate
Ferrous Sulfate
Sodium Chloride
Manganese Sulfate
Remarks
The medium permits the growth of anaerobic
bacteria, especially lactobacilli and enables to
differentiate various bacteria easily because
they will each form characteristic colonies.
Bubble Absorbing Agent (Silicon)
Polysorbate 80
L-Cysteine Hydrochloride
Agar
8-12
In 58.0 g/L
2.4 g
10.0 g
5.0 g
3.0 g
5.0 g
3.2 g
10.0 g
0.5 g
1.0 g
1.0 g
0.2 g
0.01 g
0.01 g
0.007 g
0.2 g
1.0 g
0.5 g
15.0 g
pH 7.2 ±
Back to the Index
05430-20100215 Koh SAI
Clostridia Count Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05409
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 70.3 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes, and
keep the medium at about 55°C. Distribute
15 mL of medium into the anaerobic
culture pouch in which 10 mL of sample
has distributed. Mix well, and weld to seal
the neck of the pouch after removing air
bubbles. Incubate at 37±1°C for 24±2
hours.
Components
In 70.3 g/L
Mixed Peptone
Remarks
The medium is used for the colony counting
of Clostridia (Spore forming, sulfurous acid
reducing anaerobic bacterium) in foodstuff.
Clostridia develop colonies with brown to
black color by reducing sulfurous acid, while
others develop light white colonies.
15.0 g
Soya Peptone
7.5 g
Yeast Extract
7.5 g
Beef Extract
7.5 g
Ferric Ammonium Citrate
1.0 g
Sodium Hydrogen Sulfite
1.0 g
L-Cysteine Chloride
0.75 g
Agar
30.0 g
pH 7.6 ±
Back to the Index
8-13
05409-20100215 Koh SAI
NGKG Agar Base “Nissui”
Code
Form
Package
Storage
Shelf Life
05282
Granule
300 g
Dry, RT
3 years
do not show the lecithinase reaction.
Directions
Remarks
Suspend 26.5 g of the dehydrated medium in 900
mL of distilled water, mix well and heat to dissolve
the medium. Sterilize by autoclaving at 121°C for
15 minutes. Maintain the medium at about 50°C,
add 100 mL of 20% egg-yolk suspension, mix well
and distribute about 20 mL amounts into Petri
dishes.
For the preparation of egg-yolk suspension, add
20 mL of egg-yolk to 80 mL of sterilized saline
solution aseptically, and mix well.
The medium was designed for the selection and
detection of B. cereus from the contaminated
foods.
On the medium, the spores of B. cereus are
formed well. After incubation at 30°C for 18
hours, the sporulation can be determined by
observing them microscopically.
Formula
Components
Peptone
Yeast Extract
Sodium Chloride
Glycine
Polymyxin B Sulfate
Phenol Red
Agar
Determinations
After drying the surface of the plate, smear the
specimens and incubate at 30°C for 18 – 24
hours.
B. cereus forms white and slightly thick colonies
with an irregular margin on the medium and
shows the lecithinase reaction. In case of the
lecithinase reaction, their colonies form a zone of
opacity, and the medium around the colonies
presents a red color. The growth of miscellaneous
bacteria except B. cereus may be inhibited. Even
if some bacteria grow, their colonies are small and
In 26.5 g/L
1.0 g
0.5 g
4.0 g
3.0 g
50,000 units
0.025 g
18.0 g
pH 6.8 ±
Back to the Index
9-1
05282-20100215 Koh SAI
Sensitivity Disk Agar-N “Nissui”
(For sensitivity test)
Code
Form
Package
Storage
Shelf Life
05530
Powder
300 g
Dry, RT
3 years
concentration in the medium is exactly
maintained at 0.8%.
The medium is also used for an agar plate
method.
Directions
Suspend 38.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 115°C for 15 minutes.
Distribute 20 mL into Petri dishes to attain
a thickness of 4 mm. (A round Petri dish
with diameter of 9 cm gives 4 mm
thickness with 20 mL medium).
Formula
Components
Casamino Acid
Heart Extract Powder
Soluble Starch
Dextrose
L-Tryptophan
L-Cystine
Biotin
Agar
Remarks
The medium is a modification of Mueller
Hinton medium, and does not inactivate
sulfa drugs and any other antibiotics.
The medium has a fixed quantity of calcium
ion and magnesium ion based on the
recommendation of WHO, and provides a
correct inhibitory zone against
aminoglycosides and tetracyclines.
Since the salt concentration affects the
diffusion of the antibiotics, the salt
In 38.0 g/L
16.5 g
3.0 g
1.5 g
2.0 g
0.05 g
0.05 g
5 μg
15.0 g
pH 7.3 ±
Back to the Index
10-1
05530-20100215 Koh SAI
Mueller-Hinton Agar-N “Nissui”
(For isolation of general bacteria)
Code
Form
Package
Storage
Shelf Life
05533
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 38.0 g of the dehydrated medium
in 1,000 mL of distilled water; mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes. Use
for plates and thickness of medium shall be
4 mm (for 9 cm plate, pour 25 mL of
autoclaved medium).
Components
In 38.0 g/L
Beef extract
2.0 g
Casamino acid
17.5 g
Soluble starch
1.5 g
Agar
17.0 g
pH 7.3 ±
Remarks
This medium is also fit for Anti-susceptibility
testing which applicable for K-B method
(following to NCCLS document M2-A6).
Back to the Index
10-2
05533-20100215 Koh SAI
Sensitivity Test Broth “Nissui”
(For MIC tests)
Code
Form
Package
Storage
Shelf Life
05534
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 23.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute an
adequate amount into test tubes or flasks,
and sterilize by autoclaving at 115°C for 15
minutes.
Components
In 23.0 g/L
Casamino Acid
Remarks
The medium is a modification of Mueller
Hinton medium, and does not inactivate
sulfa drugs and any other antibiotics. The
medium is used when fluid medium is
preferable.
16.5 g
Heart Extract Powder
3.0 g
Soluble Starch
1.5 g
Dextrose
2.0 g
L-Tryptophan
0.05 g
L-Cystine
0.05 g
Biotin
5 μg
pH 7.4 ±
Storage
Back to the Index
The medium is extremely hygroscopic, and
stored in the desiccator closed tightly.
10-3
0553420100215 Koh SAI
Heart Infusion Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05503
Granule
300 g
Dry, RT
3 years
Directions
Formula
Suspend 40.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes.
Distribute into Petri dishes or test tubes
according to the purpose.
Components
In 40.0 g/L
Heart Extract Powder
10.0 g
Peptone
10.0 g
Sodium Chloride
5.0 g
Agar
Remarks
15.0 g
pH 7.4 ± 0.2
Since the medium has an excellent ability to
support the growth of bacteria, it is suitable
for the cultivation of many fastidious
pathogenic bacteria.
Since E. coli, Salmonella and Shigella,
which frequently mutate, are stable during
the preservation on this medium, it is useful
for the test of their antigenicity and
virulence.
Back to the Index
10-4
05503-20100215 Koh SAI
Heart Infusion Broth “Nissui”
Code
Form
Package
Storage
Shelf Life
05505
Powder
100 g
Dry, RT
3 years
Directions
Formula
Suspend 25.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute into
adequate containers according to the
purpose, and sterilize by autoclaving at
121°C for 15 minutes.
Components
In 25.0 g/L
Heart Extract Powder
10.0 g
Peptone
10.0 g
Sodium Chloride
5.0 g
pH 7.2 ± 0.1
Remarks
Since the medium has an excellent ability to
support the growth of many kinds of
bacteria, it is suitable for the cultivation and
preservation of many fastidious bacteria.
The medium does not affect the biological
nature of bacteria, and it is also suitable for
precise biological tests.
The medium may be employed in the
routine cultivation and for various other
purposes, because the medium does not
contain any carbohydrate such as dextrose.
Back to the Index
10-5
05507-20100215 Koh SAI
Brain Heart Infusion Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05507
Powder
100 g
Dry, RT
3 years
Directions
Formula
Suspend 50.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes. Use
as plates or slants.
Components
Remarks
Since the medium has an excellent ability to
support the growth of many kinds of
bacteria, it is suitable for the cultivation and
preservation of many nutritionally fastidious
bacteria.
The medium does not affect the biological
nature of bacteria, and it is suitable for
precise biological tests.
In 50.0 g/L
Porcine Brain Extract
Powder
4.0 g
Porcine Heart Extract
Powder
4.0 g
Peptone
17.5 g
Dextrose
2.0 g
Sodium Chloride
5.0 g
Disodium Phosphate
2.5 g
Agar
15.0 g
pH 7.2 ±
Back to the Index
10-6
05507-20100215 Koh SAI
Brain Heart Infusion Broth “Nissui”
Code
Form
Package
Storage
Shelf Life
05509
Powder
100 g
Dry, RT
3 years
Directions
Formula
Suspend 35.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes.
Components
Remarks
Since the medium has an excellent ability to
support the growth of many kinds of
bacteria, it is suitable for the cultivation and
preservation of many nutritionally fastidious
bacteria. The medium is also used for the
cultivation of blood specimens.
The medium does not affect the biological
nature of bacteria, and it is suitable for
precise biological tests.
In 35.0 g/L
Porcine Brain Extract
Powder
4.0 g
Porcine Heart Extract
Powder
4.0 g
Peptone
17.5 g
Dextrose
2.0 g
Sodium Chloride
5.0 g
Disodium Phosphate
2.5 g
pH 7.2 ±
Back to the Index
10-7
05509-20100215 Koh SAI
Nutrient Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05514
Granule
300 g
Dry, RT
3 years
Directions
Formula
Suspend 35.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes.
The medium are used either slants or plates
for routine culture and preservation of
organisms that are not nutritionally
fastidious.
Components
In 35.0 g/L
Meat Extract
5.0 g
Peptone
10.0 g
Sodium Chloride
Agar
5.0 g
15.0 g
pH 7.0 ±
Back to the Index
10-8
05514-20100215 Koh SAI
Nutrient Broth “Nissui”
Code
Form
Package
Storage
Shelf Life
05511
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 30.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute the
medium into adequate containers, and
sterilize by autoclaving at 121°C for 15
minutes.
Components
In 30.0 g/L
Meat Extract
5.0 g
Peptone
Remarks
15.0 g
Sodium Chloride
5.0 g
Dipotassium Phosphate
5.0 g
pH 7.0 ±
The medium aims especially at its
transparency, and it can be widely used as a
broth medium for the cultivation of general
bacteria.
Back to the Index
10-9
05511-20100215 Koh SAI
Trypto-Soya Agar (SCD Agar) “Nissui”
(Soybean-Casein-Digest Agar)
Code
Form
Package
Storage
Shelf Life
05516
Granule
300 g
Dry, RT
3 years
Directions
Formula
Suspend 40.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes. Use
as plates or slants.
Components
In 40.0 g/L
Peptone
Remarks
15.0 g
Soya Peptone
5.0 g
Sodium Chloride
5.0 g
Agar
The medium is fit for various purposes
because it permits the growth of fastidious
bacteria that do grow on Nutrient Agar or
Heart Infusion Agar, and is markedly
superior to conventional media as a basal
medium of blood agar.
15.0 g
pH 7.3 ± 0.2
Back to the Index
10-10
05516-20100217 Koh SAI
Trypto-Soya Broth (SCD Broth) “Nissui”
(Soybean-Casein-Digest Broth)
Code
Form
Package
Storage
Shelf Life
05630
Granule
300 g
Dry, RT
3 years
It is possible to isolate the bacteria from
bacteremia and septicemia.
Directions
Suspend 30.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute into
appropriate containers, and sterilize by
autoclaving at 121°C for 15 minutes.
Formula
Components
In 30.0 g/L
Peptone
Remarks
The medium is commonly used for the
cultivation of fastidious bacteria such as
Neisseria, Brucella, streptococci and
pneumococci.
As the bacteria grow rapidly on the medium,
they tend to die out soon. So the medium is
not suitable for use a stock culture medium.
It is not desirable to subculture
pneumococci, streptococci and Neisseria
repeatedly on this medium.
For blood culture, inoculate about 5 ml of
blood specimen into 50 ml of the medium.
20.0 g
Sodium Chloride
5.0 g
Dextrose
2.5 g
Dipotassium Phosphate
2.5 g
pH 7.3 ± 0.2
Back to the Index
10-11
05630-20100215 Koh SAI
Buffered Sodium Chloride Peptone Solution (pH7.0) “Nissui”
(For preparation of sample solution)
Code
Form
Package
Storage
Shelf Life
05528
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 16.1 g of the dehydrated medium
in 1,000 mL of distilled water.
Heat to dissolve the medium, and dispense
into appropriate apparatus according to its
purpose. Sterilize by autoclaving at 121°C
for 20 minutes for use. If necessary to
dissolve sample, 1-10 g Polysorbate 20 or
Polysorbate 80 may be added to 1,000 mL
of this Buffer Solution. The Buffer Solution
is used for dissolution or dilution of sample
to prepare sample solution.
Components
In 16.1 g/L
Peptone
1.0 g
Sodium Chloride
4.3 g
Potassium Dihydrogen
Phosphate
3.56 g
Disodium Hydrogen
Phosphate
7.23 g
pH 7.0 ± 0.2
Back to the Index
10-12
05528-20100218 Koh SAI
Dorset Egg Medium “Nissui”
(For maintenance of organisms)
Code
Form
Package
Storage
Shelf Life
05522
Solid
100 tubes
Dark, Cool
5 years
Directions
Remarks
Smear on the slant and incubate at 37°C with the
rubber stopper loosened (In case of Mycobacterium
species,replace the rubber stopper with a cotton plug,
which is again replaced with a rubber stopper after
several days.) After growth, put the rubber stopper
tightly and keep at an adequate temperature.
This medium is the modification of the egg medium
described by Dorset for isolation and cultivation of
Mycobacterium species. The modification is performed
by replacing broth with physiological saline solution.
Use for the following purposes:
Maintenance of organisms. (By using the medium
strains can be preserved with the least variations of
properties such as S-R variations, colicinogenicity,
colicin tolerance and drug sensitivity.)
Pure culture of Mycobacterium species.
Determination of proteolytic activity of prganisms.
Determinations
A method of maintenance of organisms:
The temperature and term for maintenance of
organisms on this medium vary with strains of bacteria.
Maintenance of strains should be conducted in the
following technique.
Mycobacterium: Incubate at 37°C and store at room
temperature (15-25°C). Subculture every 6 months.
Nocardia: Apply in the same method as in case of
Mycobacterium, except for subcultivation of every 3
months.
Meningococci: Incubate at 37°C and store at 4°C.
Enterobacteriaceae: Subculture Salmonella once every
year, and Shigella or Escherichia coli every 6 months.
Notes:
This medium is not suitable for the preservation of
organisms which produce protease (for example, Vibrio
and Pseudomonas).
Formula
Components
Fresh Egg Mixture
(yolks and whites)
0.9% Sodium Chloride
Solution
In 1 L
800 mL
200 mL
pH 7.5 ± 0.3
This medium is prepared by distributing 3 mL amounts
into narrow test tubes, solidifying into a slant after
sterilization.
Back to the Index
10-13
05522-20100218 Koh SAI
CLED Agar “Nissui”
(Cystine-Lactose-Electrolyte Deficient Aagar, Isolation and quantitative culture of urinary organisms )
Code
Form
Package
Storage
Shelf Life
05527
Powder
300 g
Dry, RT
3 years
as it is deficient in electrolyte.
MacConkey Agar or DHL Agar was often used
for the examination of urinary bacteria, and
these media inhibit the growth of cocci. For
that reason, blood agar and nutrient agar
should be used concurrently.
The medium, however, permits an accurate
count of viable urinary organisms without
inhibiting the growth of gram-positive bacteria,
as it contains only 0.002% of brom thymol
blue.
Directions
Suspend 36.2 g of the dehydrated medium in
1,000 mL of distilled water, mix well and heat
to dissolve the medium. Sterilize by autoclaving
at 121°C for 15 minutes. After sterilization,
mix the medium well and distribute about 20
mL of the medium into Petri dishes.
Determinations
After drying the surface of the plate, smear
urinary specimens quantitatively and incubate
at 37°C for 18 – 24 hours. Calculate viable
organisms in the specimen by the number of
growing colonies on the plate.
While lactose fermenters such as E. coli,
Klebsiella and Enterobacter form yellow
colonies, lactose non-fermenters such as
Proteus and Salmonella form blue colonies.
Enterococci and staphylococci form small,
yellow colonies. Pseudomonas aeruginosa
forms green colonies with a rough periphery.
Formula
Components
Peptone
Meat Extract
Tryptone
Lactose
L-Cystine
Brom Thymol Blue
Agar
Remarks
In 36.2 g/L
4.0 g
3.0 g
4.0 g
10.0 g
0.128 g
0.02 g
15.0 g
PH 7.3 ±
The medium prevents the swarming of Proteus,
10-14
Back to the Index
05527-20100218 Koh SAI
TGC Medium, Fluid “Nissui”
Code
Form
Package
Storage
Shelf Life
05601
Granule
300 g
Dry, RT
3 years
testing of injections.
Directions
Suspend 29.3 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute
necessary amounts of medium into test
tube, sterilize by autoclaving at 121°C for
20 minutes, and immediately cool down for
use.
The prepared medium should not be used if
more than one thirds of the upper part of
the medium turned carmine.
Add the test sample, mix thoroughly with
the medium and incubate at 30 – 35°C at
least for 14 days. If it is difficult to
determine the result, transfer a part of the
culture into newly prepared medium and
sub culture. Incubate at 30 – 35°C at least
for 7 days before a final determination.
Formula
Components
In 29.3 g/L
L-Cystine
0.5 g
Sodium Chloride
2.5 g
Dextrose
5.0 g
Yeast Extract
5.0 g
Peptone
15.0 g
Sodium Thioglycollate
Resazurin
0.5 g
0.001 g
Agar
0.75 g
pH 7.1 ±
Remarks
Back to the Index
The medium is most suitable for sterility
10-15
0560120100218 Koh SAI
TGC Medium without Indicator, Liquid “Nissui”
Code
Form
Package
Storage
Shelf Life
05610
Powder
300 g
Dry, RT
3 years
difficult to use TGC Medium due to turbidity or
high viscosity of the test samples. As it does
Directions
Suspend 28.5 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute the
medium into appropriate containers.
Sterilize by autoclaving at 121°C for 20
minutes, and immediately cool down for
use. When the medium is kept for stored,
re-heat the prepared medium in boiling
water to degas before use.
Add the test sample, mix thoroughly with
the medium and incubate anaerobically at
30 – 35°C at least for 14 days. If it is
difficult to determine the result, transfer a
part of the culture into newly prepared
medium and sub culture. Incubate at 30 –
35°C at least for 7 days before a
determination.
not contain agar, it is easy to mix the
samples with the medium.
Formula
Components
In 28.5 g/L
L-Cystine
0.5 g
Sodium Chloride
2.5 g
Peptone
15.0 g
Yeast Extract
5.0 g
Dextrose
5.0 g
Sodium Thioglycollate
0.5 g
pH 7.1 ±
Back to the Index
Remarks
The medium is used for the cases in which it is
10-16
0561020100218 Koh SAI
TGC Medium without Indicator, Fluid “Nissui”
Code
Form
Package
Storage
Shelf Life
05629
Granule
300 g
Dry, RT
3 years
It is also possible to culture Trichomonas
vaginalis by adding serum 10% of the
medium.
Directions
Suspend 30.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute
about 18 mL of the medium into middle
size of tubes. Sterilize by autoclaving at
121°C for 15 minutes, and immediately
cool down for use.
When the medium is kept for stored, re-heat
the prepared medium in boiling water to
degas and rapidly cool down before use.
Formula
Components
Peptone
Remarks
The medium is modified by Vera for clinical
use, and capable to grow wide range of
bacteria from aerobic to anaerobic
microorganism, and also used for blood
culture. Do not leave any positive specimen
in the medium, because they may be killed
by a group of dextrose fermentation
bacteria that grow utilizing dextrose in the
medium.
In 30.0 g/L
17.0 g
Soya Peptone
3.0 g
Dextrose
6.0 g
Sodium Chloride
2.5 g
Agar
0.7 g
Sodium Thioglycollate
0.5 g
L-Cystine
0.25 g
Sodium sulfite
0.1 g
pH 7.1 ± 0.2
10-17
Back to the Index
05629-20100218 Koh SAI
Dextrose Peptone Broth “Nissui”
(Aseptic Test for Fungi (Mold and Yeast))
Code
Form
Package
Storage
Shelf Life
05602
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 28.5 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute into
appropriate containers, and sterilize by
autoclaving at 121°C for 20 minutes.
Incubate at 20 – 25°C for at least 10 days.
Components
In 28.5 g/L
Dextrose
Remarks
If the growth of fungi were not distinctive,
subculture into the fresh medium for
continued incubation at 20 – 25°C for more
than 10 days for final evaluation.
20.0 g
Yeast Extract
2.0 g
Magnesium Sulfate
0.5 g
Peptone
5.0 g
Potassium Dihydrogen
Phosphate
1.0 g
pH 5.7 ±
Back to the Index
10-18
05602-20100219 Koh SAI
Standard Method Agar “Nissui”
(SPC: Standard Plate Count (For viable count))
Code
Form
Package
Storage
Shelf Life
05618
Granule
300 g
Dry, RT
3 years
Directions
Formula
Suspend 23.5 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes.
Maintain the medium at about 50°C.
Distribute about 20 mL into Petri dishes
contained test samples in advance, and mix
well. Incubate at 35±1°C for 48±3 hours.
Components
In 23.5 g/L
Yeast Extract
2.5 g
Peptone
5.0 g
Dextrose
1.0 g
Agar
15.0 g
pH 7.0 ±
Remarks
Back to the Index
The medium is applied for the following
purposes; enumeration of bacteria in milk,
dairy products, soft drink powder, chipped
ice, ice cream, oysters, frozen fishes and
seafood products and others. Also for
enumeration of thermo-resistant bacteria
(spores) in sugar, starch and spices to be
used for the products of fish and meat.
10-19
05618-20100219 Koh SAI
Plate Count Agar with BCP “Nissui”
(Plate Count Agar with Brom Cresol Purple, For enumeration of the numbers of lactobacilli)
Code
Form
Package
Storage
Shelf Life
05622
Powder
300 g
Dry, RT
2 years
Color of the medium may be changed to
purple when incubation is prolonged, as the
medium becomes alkaline due to the
growth of bacteria. Bifidobacterium does
not grow on the medium, and it is
recommended to incubate it anaerobically
with BL Agar medium.
Directions
Suspend 24.7 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes.
Maintain the medium at about 50°C.
Distribute about 20 mL into Petri dishes
contained the test sample in advance, mix
well, and incubate at 35 – 37°C for 72±3
hours.
Formula
Components
Yeast Extract
Peptone
Dextrose
Polysorbate 80
L-Cysteine
Brom Cresol Purple
Agar
Remarks
Lactobacilli grown on the medium form
colonies with yellowish peripheries in the
depth and on the surface of the medium.
As polysorbate 80 and L-cysteine are being
added to the composition, the ability of the
medium with supports the growth of
lactobacilli has been improved. As
Lactobacillus bulgaricus invariably forms
yellowish colonies, its detection and
determination are easy.
In 24.7 g/L
2.5 g
5.0 g
1.0 g
1.0 g
0.1 g
0.06 g
15.0 g
PH 7.0 ±
Back to the Index
10-20
05622-20100223 Koh SAI
CVT Agar “Nissui”
(Viable counting for psychrophilic microorganism)
Code
Form
Package
Storage
Shelf Life
05625
Powder
100 g
Dry, RT
3 years
is dissolved.
②Whole medium may change to red when
the sample contains certain products that
reduce TTC.
Directions
Suspend 23.5 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Do not
autoclave.
Formula
Remarks
The medium is developed by Olson to
measure the viable count of psychrophilic
microorganism in milk and dairy products,
mainly non-fermentative gram negative rod
such as Pseudomonas.
After incubation at 20 – 25°C for 48 – 72
hours, gram negative rods such as
Pseudomonas, Acinetobacter,
Flavobacterium, Alcaligenes and Coliforms
develop red colonies by reducing TTC.
Growths of gram-positive bacteria are
inhibited.
①The least heating is must to keep quality.
Stop heating immediately after the medium
Components
In 23.5 g/L
Yeast Extract
2.5 g
Peptone
5.0 g
Dextrose
1.0 g
Crystal Violet
0.002 g
Triphenyl-TetrazoliumChloride (TTC)
0.05 g
Agar
15.0 g
pH 7.0 ±
Back to the Index
10-21
05625-20100223 Koh SAI
Lactose Broth “Nissui”
Uninoculated
Code
Form
Package
Storage
Shelf Life
05634
Granule
300 g
Dry, RT
3 years
coliform test is negative. In case of positive
gas evolution (color of medium changes to
yellow), further confirmation test with BGLB
Broth and/or EMB Agar should be
performed.
Directions
Suspend 18.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute the
medium into middle size of test tubes
including a fermentation tube or a
Durham’s tube. Sterilize by autoclaving at
121°C for 15 minutes, and cool down
rapidly for use. Do not use any tubes that
contain bubbles in a fermentation tube or a
Durham’s tube.
To inoculate the test sample of more than
10 mL, use twice or three times thicker
medium.
Formula
Components
In 18.0 g/L
Meat Extract
3.0 g
Peptone
10.0 g
Lactose
5.0 g
Brom Thymol Blue
0.024 g
pH 7.0 ±
Remarks
The medium is used for testing the
presence of coliform in food and water, and
is also used for the determination of lactose
fermentative bacteria.
When no gas was evolved after incubation,
Back to the Index
11-1
05634-20100223 Koh SAI
BGLB Broth “Nissui”
Uninoculated
Code
Form
Package
Storage
Shelf Life
05638
Granule
300 g
Dry, RT
3 years
fermentative bacteria.
When gas was evolved after incubation,
coliform test is positive.
In case of the examination of water,
incubate in Lactose Broth first, and when
the production of gas is observed,
transplant the culture to BGLB Broth.For the
test of food and drinks, add the specimen
directly to the medium and observer the
production of gas.
The medium inhibits the growth of grampositive bacteria, while it permits the
proliferation of E. coli.
Directions
Suspend 40.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute the
medium into middle size of test tubes
containing a fermentation tube or a
Durham’s tube. Sterilize by autoclaving at
121°C for 15 minutes, and cool down
rapidly for use. Do not use any tubes that
contain bubbles in a fermentation tube or a
Durham’s tube.
To inoculate the test sample of more than
10 mL, use double concentration medium.
Keep strictly a sterilization time, and ensure
a rapid cooling before use.
Slight color discrepancy by lot to lot may be
observed.
Formula
Components
Calf Bile Powder
Lactose
Peptone
Brilliant Green
Remarks
The medium is used for testing the
presence of coliform in food and water, and
is also used for the determination of lactose
11-2
In 40.0 g/L
20.0 g
10.0 g
10.0 g
0.0133 g
pH 7.0 ±
Back to the Index
05638-20100223 Koh SAI
EC Broth “Nissui”
Uninoculated
Code
Form
Package
Storage
Shelf Life
05648
Powder
100 g
Dry, RT
3 years
37°C for 48 hours, determine coliform to be
positive, and determine Escherichia coli to
be positive in case of evolution of gas after
incubation at 44.5°C.
The medium inhibits the growth of
Enterococcus.
Directions
In case of 1 mL or less of the specimen, use
the prescribed amount (37.0 g) of the
medium, and in case of 10 mL or more of
the specimen, use twice the amount (74.0
g) of the medium.
Suspend the dehydrated medium in 1,000
mL of distilled water, mix well and heat to
dissolve the medium. Distribute 10 mL of
medium into middle size of test tubes
containing a fermentation tube or a
Durham’s tube. Sterilize by autoclaving at
121°C for 15 minutes, and cool down
rapidly for use. Do not use any tubes that
contain bubbles in a fermentation tube or a
Durham’s tube.
Formula
Components
Remarks
The medium is used for enumeration of
coliform bacteria in water, milk and seafood
products.
When gas is evolved after incubation at
In 37.0 g/L
Peptone
20.0 g
Lactose
5.0 g
Bile Salts
1.5 g
Dipotassium Phosphate
4.0 g
Potassium Dihydrogen
Phosphate
1.5 g
Sodium Chloride
5.0 g
pH 6.9 ±
11-3
Back to the Index
05648-20100224 Koh SAI
Desoxycholate Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05636
Granule
300 g
Dry, RT
3 years
Directions
Remarks
Suspend 45.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute
about 20 mL of medium into Petri dishes. In
case of the liquid specimen, it is
recommended to use the pour plate method
to facilitate counting.
Do not autoclave.
The medium is widely used for the detection
of coliform bacilli in water, drinks and foods.
The medium does not require any
sterilization.
Formula
Components
Sodium Desoxycholate
Peptone
Ferric Ammonium Citrate
Sodium Chloride
Dipotassium Phosphate
Lactose
Neutral Red
Agar
Determination
Coliform bacilli form red colonies after
incubation at 35±1°C for 18 – 22 hours.
The medium around colonies has light
peach or brick colored precipitates.
Other bacteria are invisible or form
extremely small and colorless colonies in
general.
Proteus forms generally large colorless or
brown colonies.
The growth of gram-positive coccus is
inhibited.
In 45.0 g/L
1.0 g
10.0 g
2.0 g
5.0 g
2.0 g
10.0 g
0.033 g
15.0 g
pH 7.2 ± 0.2
Back to the Index
11-4
05636-20100224 Koh SAI
EMB Agar “Nissui”
(Eosin Methylene Blue Agar)
Code
Form
Package
Storage
Shelf Life
05644
Powder
300 g
Dry, RT
3 years
The growth of gram-positive organisms is
inhibited by the dyes.
Directions
Suspend 37.5 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes.
Distribute about 20 mL of the medium into
Petri dishes.
Formula
Components
Determinations
Incubate at 35±1°C for 22-26 hours.
E. coli, one of coliform organisms, forms
colonies of metallic luster with a black
center, while Enterobacter aerogenes forms
larger colonies with a dark center.
Salmonella and Shigella form colorless,
transparent and small colonies.
In 37.5 g/L
Peptone
10.0 g
Lactose
10.0 g
Dipotassium Phosphate
2.0 g
Eosin Y
0.4 g
Methylene Blue
0.065 g
Agar
15.0 g
pH 6.8 ± 0.2
Back to the Index
Remarks
The medium is used for the differentiation
of coliform organisms in foods and drinks.
11-5
05644-20100224 Koh SAI
X-GAL Agar “Nissui”
(For the test of Coliform group)
Code
Form
Package
Storage
Shelf Life
05631
Granule
300 g
Dry, RT
3 years
blue/blue-green color by β-galactosidase
produced by coliform group.
Directions
Suspend 44.3 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes.
Distribute 20 mL amounts of medium into
Petri dish.
For the mixing and dilution culture of liquid
specimen, the medium may be used in
“pour plate” technique.
Formula
Components
Peptone
Yeast Extract
Sodium Pyruvate
Sodium Chloride
Disodium Phosphate
Potassium nitrate
Sodium Lauryl Sulfate
Remarks
In 44.3 g/L
15.0 g
5.0 g
1.0 g
5.0 g
2.0 g
1.0 g
0.15 g
5-bromo-4-chloro-3-indolyl-βD-galactopyranoside (X-GAL)
The medium is used for the test for coliform
group in foods and drinks. Coliform group
develop a blue to blue-green colony after
incubation at 35 – 37°C for 20 ± 2 hours.
Growths of almost all other bacteria are
inhibited, and they develop only white
colony even if they grow.
X-GAL (colorimetric enzyme substrate) in
the medium is decomposed to bring out
Agar
0.15 g
15.0 g
pH 7.1 ±
Back to the Index
11-6
05631-20100224 Koh SAI
XM-G Agar “Nissui”
(Test for Coliform group and E. coli)
Code
Form
Package
Storage
Shelf Life
05632
Granule
300 g
Dry, RT
3 years
may be observed if the sample contains
lactobacilli that has also a β-galactosidase.
Directions
Suspend 39.3 g of the dehydrated medium in
1,000 mL of distilled water, mix well and heat
to dissolve the medium. Sterilize by autoclaving
at 121°C for 15 minutes. Distribute necessary
amounts of medium into Petri dish.
Formula
Components
Peptone
Sodium Pyruvate
L-Tryptophan
D-Sorbitol
Sodium Chloride
Remarks
The medium is used for the screening test for E.
coli and coliform group. E. coli has a βglucuronidase that decomposes the substrate
of X-GLUC to produce blue dye. For the
meanwhile Coliform group have aβgalactosidase that decomposes the substrate
of MAGENTA-GAL to produce red dye. E.coli has
both enzymes, and may produce a blue to blue
purple color.
Since E. coli O157 does not have a βglucuronidase, they are identified as a Coliform
group.
Incubate at 35-37°C for 20±2 hours. Over-time
incubation may foster growth of microorganism
other than E. coli and Coliform group. Red color
Sodium dihydrogen Phosphate
Disodium Phosphate
Potassium nitrate
Sodium Lauryl Sulfate
In 39.3 g/L
10.0 g
1.0 g
1.0 g
1.0 g
5.0 g
2.2 g
2.7 g
1.0 g
0.2 g
5-bromo-4-chloro-3-indolyl-βD-glucuronide (X-GLUC)
5-bromo-6-chloro-3-indolyl-β-Dgalactopyranoside (MAGENTA-GAL)
Agar
0.1 g
0.1 g
15.0 g
pH 7.0 ± 0.2
Back to the Index
11-7
05632-20100224 Koh SAI
Blue Light Broth “Nissui”
(Rapid test for Coliform group and E. coli)
Code
Form
Package
Storage
Shelf Life
05607
Granule
300 g
Dry, RT
3 years
isolate a fluorescence substance of 4-MethylUmbelliferone.
Directions
Suspend 17.4 g of the dehydrated medium in
1,000 mL of distilled water, mix well and heat
to dissolve the medium. Distribute necessary
amounts of medium into test tube. Sterilize by
autoclaving at 121°C for 15 minutes.
Formula
Remarks
The medium is used for the test for coliform
group and E. coli in foodstuffs or water.
Coliform group change the medium blue to
blue-green after incubation at 35 – 37°C for 24
hours. Fluorescent is observed when the
positive medium is exposed to UV light (365
nm), if E. coli was present.
X-GAL (colorimetric enzyme substrate) in the
medium is decomposed to bring out blue/bluegreen color by β-galactosidase produced by
coliform group.
MUG in the medium is decomposed byβGlucuronidase to be produced by E. coli to
Components
Peptone
Sodium Chloride
Sodium Pyruvate
Potassium dihydrogen
phosphate
In 17.4 g/L
5.0 g
5.0 g
1.0 g
Dipotassium Phosphate
4.0 g
1.0 g
0.1 g
Potassium nitrate
Sodium Lauryl Sulfate
1.0 g
Isopropyl-β-D(-)Thiogalactopyranoside (IPTG)
0.1 g
5-bromo-4-chloro-3-indolyl-βD-galactopyranoside (X-GAL)
0.1 g
4-Methyl-Umbelliferyl-βD-Glucuronide (MUG)
0.1 g
pH 7.1 ±
Back to the Index
11-8
05607-20100225 Koh SAI
EC Blue “Nissui”
(Test for Coliform group and E. coli in water)
EC Blue 100P “Nissui”
EC Blue 100 “Nissui”
Comparator
Distilled
water
Uninoculated
E. coli
Coliform
Uninoculated
Coliform
Coliform
EC Blue 10 “Nissui”
Comparator
Code
Distilled
water
Package
05591
100P (For 100 mL)
100 Packs
05593
100 (For 100 mL)
80 Bottles
05613
10 (For 10 mL)
150 Bottles
Storage
Shelf Life
Light
shielding,
2 years
RT
1 year
MUG in the medium is decomposed by βGlucuronidase to be produced by E. coli to
isolate a fluorescence substance of 4-MethylUmbelliferone.
Directions
A special pouch of EC Blue 100P contains an
exact amount of medium for the preparation of
100 mL medium. Simple and easy use neither
weight measuring nor autoclaving.
A disposable bottle of EC Blue 100 and EC Blue
10 contains an exact amount of medium for
the preparation of 100 mL and 10 mL medium
respectively. Simple and easy use neither
weight measuring, autoclaving nor incubation
tube.
Formula
Remarks
Coliform group change the medium blue to
blue-green after incubation at 35 – 37°C for 24
hours. Fluorescent is observed when the
positive medium is exposed to UV light (365
nm), if E. coli is present. X-GAL (colorimetric
enzyme substrate) in the medium is
decomposed to bring out blue/blue-green color
by β-galactosidase produced by coliform group.
Components
Peptone
Sodium Pyruvate
Dipotassium Phosphate
Potassium nitrate
In 17.4 g/L
5.0 g
1.0 g
4.0 g
1.0 g
Isopropyl-β-D(-)Thiogalactopyranoside (IPTG)
0.1 g
5-bromo-4-chloro-3-indolyl-βD-galactopyranoside (X-GAL)
0.1 g
4-Methyl-Umbelliferyl-βD-Glucuronide (MUG)
Sodium Chloride
Sodium Lauryl Sulfate
Monopotassium Phosphate
11-9
0.1 g
5.0 g
0.1 g
1.0 g
pH 7.1 ± 0.2
Back to the Index
05591-20100225 Koh SAI
EC-Blue 100 “Nissui” Bottle Holder
Code
Form
Package
Storage
Shelf Life
06574
For 10 Bottles
1
-
-
Back to the Index
11-10
06574-20100225 Koh SAI
EC Blue 10; 100 “Nissui” Comparator
Code
Package
Storage
Shelf Life
Light
shielding,
1 year
06516
10
10 mL × 1
05617
100
100 mL × 1
Cool
Formula
Components
Indigo Carmine
o-nitrophenol
2 mg
4.8 mg
4-Methylumbelliferone
sodium salt
1 mg
Potassium Dihydrogen
Phosphate
1.0 g
Dipotassium Phosphate
Distilled Water
4g
1000 mL
Back to the Index
11-11
05617-20100225 Koh SAI
EC-Blue 100 “Nissui” MPN Plate
Code
Form
Package
Storage
Shelf Life
06517
-
18
-
-
Back to the Index
11-12
06517-20100225 Koh SAI
MacConkey Sorbitol Agar “Nissui”
(Isolation medium for E. coli O157)
Code
Form
Package
Storage
Shelf Life
05643
Granule
300 g
Dry, RT
3 years
Directions
Formula
Suspend 50.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes.
Distribute necessary amounts of medium
into Petri dish.
Components
In 50.0 g/L
Bile Salt
1.0 g
Peptone
19.0 g
D-Sorbitol
10.0 g
Sodium Chloride
Remarks
The medium is used for an isolation of E.
coli O157 based on the nature that O157
does not ferment sorbitol while other E. coli
do. E. coli O157 develops a semitransparent
colony after incubation at 37°C for 18 – 20
hours. For the meanwhile other E. coli
develop a redbrick color
colony with light peach or redbrick zone of
desoxycholic acid precipitated.
Growths of gram positive coccus are
inhibited.
5.0 g
Crystal Violet
0.001 g
Neutral Red
0.03 g
Agar
15.0 g
pH 7.2 ± 0.2
Back to the Index
11-13
05643-20100225 Koh SAI
EF Agar Base “Nissui”
(Appended reagent: TTC, For Isolation of Enterococcus)
Code
Form
Package
Storage
Shelf Life
05679
Powder
100 g
Dry, RT
3 years
Since Enterococcus is an indicator of fecal
pollution as in case of Coliform, the
detection of it is important for the survey of
fecal pollution.
Directions
Suspend 54.5 g of the dehydrated medium
together with 0.25 g of Sodium azide in
1,000 mL of distilled water, mix well and
heat to dissolve the medium. Keep at
around 60°C, and then add 10 mL of 1.5%
TTC solution. Mix well and distribute the
medium into Petri dishes.
1.5% TTC solution:
Dissolve 0.15 g of TTC (appended to the
medium) in 10 mL of distilled water, and
store in brown bottle in dark and cool place.
Formula
Components
Remarks
The medium is used for an isolation of
Enterococcus in faces, water, milk, dairy
products and processed meat.
Incubate at 37°C for 48 hours. E. faecalis
forms pink or red brown colonies 0.5 – 2
mm in diameter, while E. faecium forms
yellow colonies. Growths of gram-negative
bacilli are inhibited.
In 54.5 g/L
Calf Brain Extract Powder
8.5 g
Heart Extract Powder
8.5 g
Peptone
10.0 g
Dextrose
10.0 g
Dipotassium Phosphate
Brom Thymol Blue
2.5 g
0.032 g
2,3,5-Triphenyltetrazolium Chloride
(TTC) (Appended reagent)
0.15 g
Agar
15.0 g
pH 7.2 ±
11-14
Back to the Index
05679-20100226 Koh SAI
mEC Broth “Nissui”
(Enrichment medium for E. coli O157)
Code
Form
Package
Storage
Shelf Life
05649
Powder
300 g
Dry, RT
3 years
Directions
Formula
Suspend 36.6 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute
necessary amounts of medium into
appropriate container. Sterilize by
autoclaving at 121°C for 15 minutes, and
then add 20 mg of Novobiocin.
Incubate at 42°C for 18 – 24 hours.
Components
Remarks
The medium is used for an enrichment
culture of E. coli O157 in foodstuffs.
In 36.6 g/L
Peptone
20.0 g
Lactose
5.0 g
Bile Salt
1.12 g
Dipotassium Phosphate
4.0 g
Potassium Dihydrogen
Phosphate
1.5 g
Sodium Chloride
5.0 g
pH 6.9 ±
Back to the Index
11-15
05649-20100226 Koh SAI
Sabouraud Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05701
Granule
300 g
Dry, RT
3 years
contaminant microbes, it is desirable to use
the selective media such as Mycobiotic Agar
and Candida GE agar simultaneously for the
isolation.
Directions
Suspend 65.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes, and
use as a plate or a slant.
Formula
Components
Determinations
Incubate at 37°C or at 25°C. For the
isolation of Candida, incubate for 48 – 72
hours and in case of other fungi, incubation
for 5 days may sometimes be necessary.
Candida forms colonies with white, opaque,
and wet swelling, which gradually change to
light brown.
In 65.0 g/L
Peptone
10.5 g
Dextrose
40.0 g
Agar
14.5 g
pH 5.6 ± 0.2
Back to the Index
Remarks
Growth of Candida on the medium is so well
that it can survive for a long period without
morphological variations.
As the medium cannot inhibit the
12-1
05701-20100226 Koh SAI
Corn Meal Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05702
Powder
100 g
Dry, RT
3 years
Directions
Remarks
Suspend 17.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes.
Distribute the medium into Petri dishes, and
dry the surface before use.
As the medium has an effective facilitation
ability to form pseudohypha, it is used for
identification of fungi such as Candida
albicans that have the ability to form
chlamydospores and pseudohypha.
Formula
Determinations
Components
It is recommended to incubate at 25°C and
37°C. It is also recommended to add
Polysorbate 80 at the concentration of 1%
for the purpose to observe chlamydospores.
After incubation, examine whether
chlamydospores or pseudohypha are
present therein. The tip of hypha of
chlamydospores generally shows an oval
form of deep dark color, and pseudohypha
also shows specific forms respectively.
In 17.0 g/L
Corn Meal Percolated
Powder
Agar
2.0 g
15.0 g
pH 6.0 ±
Back to the Index
12-2
05702-20100226 Koh SAI
AC Broth Base “Nissui”
(For the test of Enterococcus)
Uninoculated
Code
Form
Package
Storage
Shelf Life
05680
Powder
100 g
Dry, RT
3 years
suspected colony on the isolation culture,
and inoculate to incubate at 45°C for 48
hours.
On the broth all gram negative and positive
bacteria except Enterococcus are inhibited.
Consequently, Enterococcus (+) is confirmed
when any growth (turbidity) is visually
observed.
Directions
Suspend 50.5 g of the dehydrated medium
together with 0.25 g of Sodium azide in
1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute 10
mL of medium into the middle size of test
tubes. Sterilize by autoclaving at 121°C for
15 minutes.
In case of sample volume is bigger than 10
mL, two or three fold concentration broth is
prepared according to the volume of
sample.
For preliminary confirmation test of
Enterococcus in water or foodstuffs,
incubate at 37°C for 48 hours. For
confirmation test, incubate at 45°C for 48
hours.(for assessment of MPN).
Formula
Components
Peptone
Yeast Extract
Dextrose
Sodium Citrate
Sodium Chloride
Disodium Phosphate
Potassium Dihydrogen
Phosphate
Remarks
For an identification of Enterococcus
obtained from clinical samples, fish a
In 50.5 g/L
20.0 g
5.0 g
5.0 g
10.0 g
5.0 g
4.0 g
1.5 g
Back to the Index
12-3
05680-20100226 Koh SAI
Candida GE Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05703
Powder
100 g
Dry, RT
3 years
Directions
Remarks
Suspend 62.0 g of the dehydrated medium in
1,000 mL of distilled water, mix well and heat
to dissolve the medium. Distribute about 20
mL amounts into Petri dishes. Do not
autoclave.
Features:
1. The medium inhibits the growth of
miscellaneous bacteria and permits the growth
of Candida specifically.
2. Nitrofuran derivative markedly or completely
inhibits gram-negative bacilli. Therefore the
medium facilitates the detection of Candida.
● Note:
It is necessary to smear the specimens heavily
on the medium.
●
●
Determinations
After drying the surface, inoculate the slime in
oral or vaginal secreta with a sterilized swab
and spread on the surface of the medium.
Incubate at 35 – 37°C for 2 – 3 days.
Candida forms specific colonies that are round
or oval in shape with a diameter of 3 – 5 mm
and are wet and opaque, and have specific odor
and luster.
C. albicans forms colonies of creamy or offwhite color after 48 – 72 hours incubation,
which gradually turn pale brownish.
C. krusei forms flat and irregularly shaped
colonies with no luster, while the other Candida
form brown or pale brown colonies.
Formula
Components
Yeast Extract
Peptone
Dextrose
Nitrofuran derivative
Agar
In 62.0 g/L
10.0 g
8.5 g
30.0 g
0.5 g
13.0 g
pH 6.0 ±
Back to the Index
12-4
05709-20100114 Koh SAI
Czapek Dox Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05705
Powder
100 g
Dry, RT
3 years
and cultivation of fungi, and is
recommended especially for the cultivation
of Aspergillus.
Directions
Suspend 53.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes, and
cool down about 50°C. Distribute about 20
mL of the medium into Petri dishes
contained the test specimen in advance,
mix and incubate at 30°C or 22°C for
about 7 days.
Formula
Components
In 53.0 g/L
Dextrose
Determinations
The fungi grown in the deep layer possess
flat, folded waves and gradually produce
color. Those grown on the surface possess
folded waves on the surface of the colonies
and produce the specific color of the fungi
with the incubation.
36.0 g
Sodium Nitrate
2.0 g
Dipotassium Phosphate
1.0 g
Magnesium Sulfate
0.5 g
Potassium Chloride
0.5 g
Ferrous Sulfate
0.01 g
Agar
13.0 g
pH 6.0 ±
Remarks
Back to the Index
The medium is used for the determination
12-5
05705-20100226 Koh SAI
Malt Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05706
Powder
100 g
Dry, RT
3 years
Directions
Formula
Suspend 45.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute into
the test tubes, sterilize by autoclaving at
121°C for 15 minutes, and use after
solidifying in a slanted position or in the
butt.
Components
In 45.0 g/L
Malt Extract
25.0 g
Agar
20.0 g
pH 5.0 ±
Back to the Index
Remarks
The medium is used for the preservation of
Saccharomyces carlsbergensis that is used
for analysis of Vitamin B6. It is also used
widely for the cultivation of common
lactobacilli, yeast and fungi.
12-6
05706-20100226 Koh SAI
Potato Dextrose Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05709
Granule
300 g
Dry, RT
2 years
chloramphenicol (100 mg/L).
Directions
Suspend 39.0 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Sterilize by
autoclaving at 121°C for 15 minutes. Use
as the plate or the pour plate method.
Incubate at about 25°C is desirable. For
mixing and dilution method, distribute the
medium into Petri dishes contained the test
specimen in advance.
Formula
Components
In 39.0 g/L
Potato Extract
3.9 g
Dextrose
21.0 g
Agar
14.1 g
pH 5.6 ± 0.2
Remarks
Back to the Index
The medium is used for the isolation of
yeast and fungi from powdered milk or
processed milk products. To inhibit the
growth of other bacteria for the purpose of
enumerating the numbers of yeast and
fungi, adjust the above pH (6.0 ±) to pH 3.5
± 0.1 (add sterilized 10% aqueous solution
of tartrate to the sterilized medium), or add
12-7
05709-20100114 Koh SAI
Lactobacilli Culture Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05800
Powder
100 g
Dry, RT
3 years
medium. This makes differentiation of
Lactobacilli easy.
Directions
Suspend 59.6 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute into
the test tube, sterilized by autoclaving at
121°C for 10 minutes, and use after
solidifying in the butt position. Stab the
medium for culture at 37°C for 24 – 48
hours. White turbidity (positive growth) will
be observed around the stab areas, which
can be preserved for 6 months in
refrigerator. More than 1-year preservation
is possible with GDO medium.
Formula
Components
Yeast Extract
Peptone
Dextrose
Potassium Dihydrogen
Phosphate
Dipotassium Phosphate
Sodium acetate
Magnesium Sulfate
Manganese Sulfate
Ferrous Sulfate
Agar
Remarks
The medium is also used for the
preservation of L. arabinosus, S. faecalis
and other Lactobacilli.
Lactobacilli develop the colony with clear
portion around the stab area when 5 – 10
g/L of calcium carbonate is added to the
In 59.6 g/L
5.5 g
12.5 g
11.0 g
0.25 g
0.25 g
10.0 g
0.1 g
5.0 mg
5.0 mg
20.0 g
pH 6.8 ±
Back to the Index
13-1
05800-20100301 Koh SAI
Lactobacilli Inoculum Broth “Nissui”
Code
Form
Package
Storage
Shelf Life
05801
Powder
100 g
Dry, RT
3 years
Directions
Formula
Suspend 39.6 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute into
the test tube, sterilized by autoclaving at
121°C for 10 minutes.
Remarks
The medium is used for the preparation of
inoculation for Lactobacilli.
Components
In 39.6 g/L
Yeast Extract
5.5 g
Peptone
12.5 g
Dextrose
11.0 g
Potassium Dihydrogen
Phosphate
0.25 g
Dipotassium Phosphate
0.25 g
Sodium acetate
10.0 g
Magnesium Sulfate
0.1 g
Manganese Sulfate
5.0 mg
Ferrous Sulfate
5.0 mg
pH 6.8 ±
Back to the Index
13-2
05801-20100301 Koh SAI
B12 Culture Agar “Nissui”
Code
Form
Package
Storage
Shelf Life
05802
Powder
100 g
Dry, RT
3 years
Directions
Formula
Suspend 49.7 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute into
the test tube, sterilized by autoclaving at
121°C for 10 minutes, and use after
solidifying in the butt position. Stab the
medium for culture at 37°C for 18-24
hours, and preserve in cool place.
Subculture 2 – 3 times a week.
Remarks
The medium is used for the preservation of
L. leichmannii for B12 quantitative analysis.
The medium becomes clouded just after
autoclaving due to high temperature, and
becomes clear when cool down.
Components
In 49.7 g/L
Yeast Extract
8.5 g
Peptone
8.5 g
Dextrose
11.0 g
Potassium Dihydrogen
Phosphate
2.0 g
Tomato Juice Powder
3.7 g
Polysorbate 80
1.0 g
Agar
15.0 g
pH 6.9 ±
Back to the Index
13-3
05802-20100301 Koh SAI
B Inoculum Broth “Nissui”
12
Code
Form
Package
Storage
Shelf Life
05803
Powder
100 g
Dry, RT
3 years
Directions
Formula
Suspend 34.7 g of the dehydrated medium
in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute into
the test tube, sterilized by autoclaving at
121°C for 10 minutes.
Remarks
The medium is used for the preparation of
inoculation for L. leichmannii for B12
quantitative analysis.
Components
In 34.7 g/L
Yeast Extract
8.5 g
Peptone
8.5 g
Dextrose
11.0 g
Potassium Dihydrogen
Phosphate
2.0 g
Tomato Juice Powder
3.7 g
Polysorbate 80
1.0 g
pH 6.9 ±
Back to the Index
13-4
05803-20100301 Koh SAI
B Assay Medium (set) “Nissui”
12
Code
Form
Package
Storage
Shelf Life
05819
6 x 2mL 10%
Polysorbate 80
50 g
Cool
3 years
Range of response:
0.01 ng~0.2 ng/tube (5 mL)
Incubation temperature should be constant
(within ±0.5°C).
Pay at most attention to preserve the
strains.
Directions
Suspend 8.3 g of the dehydrated medium in
about 80-90 mL of distilled water, mix well
and heat to dissolve the medium, and cool
down the solution. After adjusting the pH to
6.2±0.1, add 2.0 mL of Polysorbate 80, and
add distilled water to make total volume
100 mL.
Distribute into the test tube, and double the
total volume with the distilled water after
adding the sample. Sterilize by autoclaving
at 121°C for 5 minutes. Inoculate the strain
for culture at 37°C for 16 – 24 hours.
Incubate 72 hours for the purpose of
acidimetry.
Storage
Cap tightly and keep at cool place (2 –
10°C).
Formula
As directed in U.S.P.XIX (1975)
pH 6.2±0.1
Remarks
Back to the Index
Strain to be used is Lactobacillus
leichmannii ATCC7830 (available also from
Nissui)
13-5
05819-20100301 Koh SAI
Pyridoxine Assay Medium “Nissui”
Code
Form
Package
Storage
Shelf Life
05815
Powder
50 g
Cool
3 years
In case of incubating in the test tube, all
test tubes should be similarly slanted more
than 25 degree at the angle.
Directions
Suspend 13.0 g of the dehydrated medium
in about 80-90 mL of distilled water, mix
well and heat to dissolve the medium, and
cool down the solution. After adjusting the
pH to 5.2±, add distilled water to make total
volume 100 mL.
Distribute into the flask or test tube, and
double the total volume with the distilled
water after adding the sample. Sterilize by
boiling at 100°C for 10 - 20 minutes.
Inoculate the strain for culture at 30°C for
16 – 24 hours.
Storage
Cap tightly and keep at cool place (2 –
10°C).
Formula
Improved formula of Atkin et al. method
pH 5.2±0.1
Back to the Index
Remarks
Strain to be used is Saccharomyces uvarum
(carlsbergensis) strain 4228 ATCC 9080
(available also from Nissui)
Range of response:
2.5 ng~25 ng/tube (10 mL)
13-6
05815-20100301 Koh SAI
Niacin Assay Medium “Nissui”
Code
Form
Package
Storage
Shelf Life
05816
Powder
50 g
Cool
3 years
0.01 μg~0.1 μg/tube (5 mL)
0.01 μg~0.1 μg/tube (2 mL)
Directions
Suspend 7.7 g of the dehydrated medium in
about 80-90 mL of distilled water, mix well
and heat to dissolve the medium, and cool
down the solution. After adjusting the pH to
7.1±, add distilled water to make the total
volume 100 mL.
Distribute into the test tube, and double the
total volume with the distilled water after
adding the sample. Sterilize by autoclaving
at 121°C for 5 minutes. Inoculate the strain
for culture at 37°C for 16 – 24 hours.
Incubate 72 hours for the purpose of
acidimetry.
Storage
Cap tightly and keep at cool place (2 –
10°C).
Formula
As directed in N.F.XIV (1975)
pH 7.1±0.1
Back to the Index
Remarks
Strain to be used is Lactobacillus
arabinosus strain 17-5 ATCC 8014
(available also from Nissui)
Range of response:
0.02 μg~0.2 μg/tube (10 mL)
13-7
05816-20100301 Koh SAI
Folic Acid Assay Medium “Nissui”
Code
Form
Package
Storage
Shelf Life
05814
Powder
50 g
Cool
3 years
0.2 ng~2 ng/tube (5 mL)
0.05 ng~0.5 ng/tube (2 mL)
Directions
Suspend 11.4 g of the dehydrated medium
in about 50 mL of distilled water, mix well
and heat to dissolve the medium, and cool
down the solution. After adjusting the pH to
7.1±0.1, add distilled water to make the
total volume 100 mL.
Distribute into the test tube, and double the
total volume with the distilled water after
adding the sample. Sterilize by autoclaving
at 121°C for 5 minutes. Inoculate the strain
for culture at 30°C for 16 – 24 hours.
Incubate 72 hours for the purpose of
acidimetry.
Storage
Cap tightly and keep at cool place (2 –
10°C).
Formula
As directed in AOAC 12th Ed. (1975)
pH 7.1±0.1
Back to the Index
Remarks
Strain to be used is Streptococcus faecalis
“R” ATCC 8043 (available also from Nissui)
Range of response:
0.5 ng~5 ng/tube (10 mL)
13-8
05814-20100301 Koh SAI
Pantothenate Assay Medium “Nissui”
Code
Form
Package
Storage
Shelf Life
05817
Powder
50 g
Cool
3 years
0.01 μg~0.1 μg/tube (5 mL)
0.01 μg~0.05 μg/tube (2 mL)
Directions
Suspend 7.7 g of the dehydrated medium in
about 80-90 mL of distilled water, mix well
and heat to dissolve the medium, and cool
down the solution. After adjusting the pH to
7.1±, add distilled water to make the total
volume 100 mL.
Distribute into the test tube, and double the
total volume with the distilled water after
adding the sample. Sterilize by autoclaving
at 121°C for 5 minutes. Inoculate the strain
for culture at 37°C for 16 – 24 hours.
Incubate 72 hours for the purpose of
acidimetry.
Storage
Cap tightly and keep at cool place (2 –
10°C).
Formula
As directed in U.S.P. XV.
pH 7.1±0.1
Back to the Index
Remarks
Strain to be used is Lactobacillus
arabinosus strain 17-5 ATCC 8014
(available also from Nissui)
Range of response:
0.02 μg~0.2 μg/tube (10 mL)
13-9
05817-20100301 Koh SAI
Biotin Assay Medium “Nissui”
Code
Form
Package
Storage
Shelf Life
05818
Powder
50 g
Cool
3 years
0.1 ng~1 ng/tube (5 mL)
0.05 ng~0.5 ng/tube (2 mL)
Directions
Suspend 7.7 g of the dehydrated medium in
about 80-90 mL of distilled water, mix well
and heat to dissolve the medium, and cool
down the solution. After adjusting the pH to
7.1±, add distilled water to make the total
volume 100 mL.
Distribute into the test tube, and double the
total volume with the distilled water after
adding the sample. Sterilize by autoclaving
at 121°C for 5 minutes. Inoculate the strain
for culture at 37°C for 16 – 24 hours.
Incubate 72 hours for the purpose of
acidimetry.
Storage
Cap tightly and keep at cool place (2 –
10°C).
Formula
Removed biotin from the formula of
Pantothenate Assay Medium, and added
400 μg of pantothenate instead. All other
ingredients remain unchanged.
pH 7.1±0.1
Back to the Index
Remarks
Strain to be used is Lactobacillus
arabinosus strain 17-5 ATCC 8014
(available also from Nissui)
Range of response:
0.2 ng~2 ng/tube (10 mL)
13-10
05818-20100301 Koh SAI
Powdered Agar “Nissui”
(For microbiological usage)
Code
Form
Package
Storage
Shelf Life
05835
Powder
500 g
Dry, RT
5 years
Directions
Product is purified agar for microbiological
usage. It rarely contains ingredient that
inhibits growth of bacteria, and then it fits
general usage for microbiological testing.
And high jelly strength make it economical.
Remarks
Product is purified agar for microbiological
testing so that it supports bacteria growth.
Back to the Index
13-11
05835-20100301 Koh SAI
Nissui Tube Triple Sugar Iron Agar
Code
Form
Package
Storage
Shelf Life
05169
Solid
(In Tubes)
4 mL × 50
Tubes
Cool
6 Months
Formula
Components
Meat Extract
Sodium Chloride
Peptone
Lactose
Sucrose
Dextrose
Ferric Citrate
Sodium Thiosulfate
Phenol Red
Agar
In 61.4 g/L
5.0 g
5.0 g
15.0 g
10.0 g
10.0 g
1.0 g
0.2 g
0.2 g
0.02 g
15.0 g
pH 7.3 ±
Back to the Index
14-1
05169-20100301 Koh SAI
Nissui Tube Sulfide Indole Motility Medium
Code
Form
Package
Storage
Shelf Life
05170
Solid
(In Tubes)
5 mL × 50
Tubes
Cool
4 Months
Formula
Components
In 35.0 g/L
Meat Extract
3.0 g
Peptone
28.0 g
Sodium Thiosulfate
0.025 g
Ferric Ammonium Citrate
1.0 g
Agar
3.0 g
pH 7.2 ±
Back to the Index
14-2
05170-20100301 Koh SAI
Nissui Tube Lysine Indole Motility Medium
Code
Form
Package
Storage
Shelf Life
05171
Solid
(In Tubes)
5 mL × 50
Tubes
Cool
6 Months
Formula
Components
Peptone
Yeast Extract
Dextrose
L-Lysine Hydrochloride
L-Tryptophan
Brom Cresol Purple
Agar
In 30.0 g/L
12.8 g
3.0 g
1.0 g
10.0 g
0.5 g
0.02 g
2.7 g
pH 6.8 ±
Back to the Index
14-3
05171-20100301 Koh SAI
Food Stamp “Nissui”
Desoxycholate Agar (DESO)
(Simple and Easy Stamp Medium for Food Hygiene test: Coliform)
Code
06053
06052
Form
Package
Storage
Shelf Life
Cool
6 Months
30 Plates
Solid
100 Plates
Directions
Formula
Food Stamp is a prepared agar medium for
Stamp method, on which agar stands up
slightly above the rim of special Petri dish of
10 cm2. Take off the cap of Food Stamp and
gently press the medium against the
surface of specimen. The surface of agar is
elastic enough to be pressed firmly against
the specimen. Press against the different
parts of the specimen when several kinds of
Food Stamps are tested simultaneously. Put
the cap again immediately after pressing.
Incubate at 35-37°C for 24 hours.
Components
Sodium Desoxycholate
Peptone
Ferric Ammonium Citrate
Sodium Chloride
Dipotassium Phosphate
Lactose
Neutral Red
Agar
In 45.0 g/L
1.0 g
10.0 g
2.0 g
5.0 g
2.0 g
10.0 g
0.033 g
15.0 g
pH 7.2 ± 0.2
Back to the Index
Interpretations
Count all colonies on the surface.
Storage
Keep dry at 4 – 10°C. Do not freeze.
15-1
06053-20100301 Koh SAI
Food Stamp “Nissui”
X-GAL Agar (XGAL)
(Simple and Easy Stamp Medium for Food Hygiene test: Coliform)
Code
06764
06763
Form
Package
Storage
Shelf Life
Cool
5 Months
30 Plates
Solid
100 Plates
Directions
Interpretations
Food Stamp is a prepared agar medium for Stamp
method, on which agar stands up slightly above
the rim of special Petri dish of 10 cm2 . Take off
the cap of Food Stamp and gently press the
medium against the surface of specimen. The
surface of agar is elastic enough to be pressed
firmly against the specimen. Press against the
different parts of the specimen when several
kinds of Food Stamps are tested simultaneously.
Put the cap again immediately after pressing.
Incubate at 35-37°C for 24 hours.
Coliform group decompose X-GAL (colorimetric
enzyme substrate) in the medium to bring out blue
/ blue-green color by β-galactosidase produced by
Coliforms.
Growth of all other bacteria will be inhibited, and
they develop only white colonies even are they
grow.
Count all blue / blue-green color colonies grown
on the surface.
Storage
Keep dry at 4 – 10°C. Do not freeze.
Formula
Components
Peptone
Yeast Extract
Sodium Pyruvate
Sodium Chloride
Disodium Phosphate
Potassium nitrate
Sodium Lauryl Sulfate
In 44.3 g/L
15.0 g
5.0 g
1.0 g
5.0 g
2.0 g
1.0 g
0.15 g
5-bromo-4-chloro-3-indolyl-βD-galactopyranoside (X-GAL)
Agar
15-2
0.15 g
15.0 g
pH 7.1 ±
Back to the Index
06764-20100302 Koh SAI
Food Stamp “Nissui”
Standard Method Agar (SMA)
(Simple and Easy Stamp Medium for Food Hygiene test: Total Viable Bacterial Count)
Code
06051
06050
Form
Package
Storage
Shelf Life
Cool
6 Months
30 Plates
Solid
100 Plates
Directions
Interpretations
Food Stamp is a prepared agar medium for
Stamp method, on which agar stands up
slightly above the rim of special Petri dish of
10 cm2. Take off the cap of Food Stamp and
gently press the medium against the
surface of specimen. The surface of agar is
elastic enough to be pressed firmly against
the specimen. Press against the different
parts of the specimen when several kinds of
Food Stamps are tested simultaneously.
Put the cap again immediately after
pressing.
This Food Stamp is designed to measure
and detect the degree of contamination of
the specimen.
Incubate at 35-37°C for 24 - 48 hours.
Count all colonies grown on the surface.
Storage
Keep dry at 4 – 10°C. Do not freeze.
Formula
Components
In 23.5 g/L
Yeast Extract
2.5 g
Peptone
5.0 g
Dextrose
1.0 g
Agar
15.0 g
pH 7.0 ±
Back to the Index
15-3
06051-20100302 Koh SAI
Food Stamp “Nissui”
XM-G Agar (XM-G)
(Simple and Easy Stamp Medium for Food Hygiene test: Escherichia coli and Coliforms)
Code
06776
06775
Form
Package
Storage
Shelf Life
Cool
5 Months
30 Plates
Solid
100 Plates
Directions
Formula
Food Stamp is a prepared agar medium for Stamp method, on which
agar stands up slightly above the rim of special Petri dish of 10 cm 2.
Take off the cap of Food Stamp and gently press the medium against
the surface of specimen. The surface of agar is elastic enough to be
pressed firmly against the specimen. Press against the different parts
of the specimen when several kinds of Food Stamps are tested
simultaneously. Put the cap again immediately after pressing.
Incubate at 35°C for 20±2 hours.
Coliform group decompose X-GAL (colorimetric enzyme substrate) in
the medium to bring out blue / blue-green color byβ-galactosidase
produced by Coliforms.
Growth of all other bacteria will be inhibited, and they develop only
white colonies even are they grow.
Components
Peptone
Sodium Pyruvate
L-Tryptophan
D-Sorbitol
Sodium Chloride
Sodium dihydrogen Phosphate
Disodium Phosphate
Potassium nitrate
Sodium Lauryl Sulfate
Interpretations
Count all pink / red-purple color colonies grown on the surface as
Coliforms.
Count all blue / blue-purple color colonies grown on the surface as E.
coli.
E. coli O-157 does not have a β-glucuronidase, and then it will be
identified as a Coliform.
Overtime incubation may foster growth of microorganisms other than
E. coli and Coliforms.
Red color may be observed if the sample contains lactobacilli that has
also a β-galactosidase.
In 39.3 g/L
10.0 g
1.0 g
1.0 g
1.0 g
5.0 g
2.2 g
2.7 g
1.0 g
0.2 g
5-bromo-4-chloro-3-indolyl-βD-glucuronide (X-GLUC)
5-bromo-6-chloro-3-indolyl-β-Dgalactopyranoside (MAGENTA-GAL)
Agar
Storage
0.1 g
0.1 g
15.0 g
pH 7.0 ± 0.2
Back to the Index
Keep dry at 4 – 10°C. Do not freeze.
15-4
06776-20100302 Koh SAI
Food Stamp “Nissui”
TCBS Agar (TCBS)
(Simple and Easy Stamp Medium for Food Hygiene test: Vibrio parahaemolyticus)
Code
06055
06054
Form
Package
Storage
Shelf Life
Cool
6 Months
30 Plates
Solid
100 Plates
alginolyticus) are observed.
Directions
Storage
Food Stamp is a prepared agar medium for
Stamp method, on which agar stands up
slightly above the rim of special Petri dish of 10
cm2 . Take off the cap of Food Stamp and gently
press the medium against the surface of
specimen. The surface of agar is elastic enough
to be pressed firmly against the specimen.
Press against the different parts of the
specimen when several kinds of Food Stamps
are tested simultaneously. Put the cap again
immediately after pressing.
Incubate at 35-37°C for 24 hours.
Keep dry at 4 – 10°C. Do not freeze.
Formula
Components
Yeast Extract
Peptone
Saccharose
Sodium Thiosulfate
Sodium Citrate
Sodium Cholate
Ferric Citrate
Sodium Chloride
Oxgall
Brom Thymol Blue
Thymol Blue
Agar
Interpretations
Green (V. parahaemolyticus) and Yellow (V.
alginolyticus) colonies are observed on the
surface.
Possible contamination of V. parahaemolyticus
is suspected when many yellow colonies (V.
15-5
In 86.0 g/L
5.0 g
10.0 g
17.0 g
10.0 g
10.0 g
3.0 g
1.0 g
10.0 g
5.0 g
0.04 g
0.04 g
15.0 g
pH 8.8 ±
Back to the Index
06055-20100302 Koh SAI
Food Stamp “Nissui”
TGSE Agar (TGSE)
(Simple and Easy Stamp Medium for Food Hygiene test: Staphylococcus aureus)
Code
06057
06056
Form
Package
Storage
Shelf Life
Cool
6 Months
30 Plates
Solid
100 Plates
not interpreted as S. aureus.
Directions
Storage
Food Stamp is a prepared agar medium for
Stamp method, on which agar stands up
slightly above the rim of special Petri dish of 10
cm2 . Take off the cap of Food Stamp and gently
press the medium against the surface of
specimen. The surface of agar is elastic enough
to be pressed firmly against the specimen.
Press against the different parts of the
specimen when several kinds of Food Stamps
are tested simultaneously. Put the cap again
immediately after pressing.
Incubate at 35-37°C for 48 hours.
Keep dry at 4 – 10°C. Do not freeze.
Formula
Components
Meat Extract
Peptone
Soya Peptone
Sodium Chloride
Lithium Chloride
Calcium Chloride
Mannitol
Glycine
Potassium Tellurite
Egg yolk
Agar
Interpretations
Staphylococcus aureus forms black colonies
with milky surroundings around the colonies.
S. aureus shows a positive egg yolk reaction.
Black colony with negative egg yolk reaction is
15-6
In 1 L
3.0 g
15.0 g
2.0 g
65.0 g
5.0 g
1.0 g
5.0 g
5.0 g
0.1 g
5%
15.0 g
pH 7.0 ± 0.2
Back to the Index
06057-20100302 Koh SAI
Food Stamp “Nissui”
MLCB Agar (MLCB)
(Simple and Easy Stamp Medium for Food Hygiene test: Salmonella)
Code
06751
06750
Form
Package
Storage
Shelf Life
Cool
4 Months
30 Plates
Solid
100 Plates
Purple colonies are not Salmonella.
Directions
Storage
Food Stamp is a prepared agar medium for
Stamp method, on which agar stands up
slightly above the rim of special Petri dish of 10
cm2 . Take off the cap of Food Stamp and gently
press the medium against the surface of
specimen. The surface of agar is elastic enough
to be pressed firmly against the specimen.
Press against the different parts of the
specimen when several kinds of Food Stamps
are tested simultaneously. Put the cap again
immediately after pressing.
Incubate at 35-37°C for 24 hours.
Keep dry at 4 – 10°C. Do not freeze.
Formula
Components
Yeast Extract
Peptone
Heart Extract Powder
Sodium Chloride
Mannitol
L-Lysine Hydrochloride
Sodium Thiosulfate
Ferric Ammonium Citrate
Brilliant Green
Crystal Violet
Agar
Interpretations
Salmonella produces hydrogen sulfide and
forms black colonies or colonies with black
center.
Citrobacter may develop black colonies just like
Salmonella.
15-7
In 49.0 g/L
5.0 g
10.0 g
2.0 g
4.0 g
3.0 g
5.0 g
4.0 g
1.0 g
0.0125 g
0.01 g
15.0 g
pH 6.8 ±
Back to the Index
06751-20100302 Koh SAI
Food Stamp “Nissui”
Cereus Agar (CERE)
(Simple and Easy Stamp Medium for Food Hygiene test: Bacillus cereus)
Code
06753
06752
Form
Package
Storage
Shelf Life
Cool
6 Months
30 Plates
Solid
100 Plates
Small colonies with negative egg yolk
reaction are not B. cereus.
Directions
Food Stamp is a prepared agar medium for
Stamp method, on which agar stands up
slightly above the rim of special Petri dish of
10 cm2 . Take off the cap of Food Stamp and
gently press the medium against the surface
of specimen. The surface of agar is elastic
enough to be pressed firmly against the
specimen. Press against the different parts of
the specimen when several kinds of Food
Stamps are tested simultaneously. Put the
cap again immediately after pressing.
Incubate at 35-37°C for 24 hours.
Storage
Keep dry at 4 – 10°C. Do not freeze.
Formula
Components
Heart Extract Powder
Proteose Peptone
Peptone
Sodium Chloride
Lactose
Glycine
Phenol Red
Polymyxin B
Egg Yolk Suspension
Agar
Interpretations
Bacillus cereus forms white colonies with an
irregular rim, which develop opaque zone
(positive egg yolk reaction) around the
colonies and change the color of medium to
Red.
15-8
In 1 L
5.0 g
10.0 g
10.0 g
5.0 g
10.0 g
10.0 g
0.05 g
50,000 units
10%
20.0 g
pH 7.1 ± 0.3
Back to the Index
06753-20100303 Koh SAI
Food Stamp “Nissui”
X-SA Agar (X-SA)
(Simple and Easy Stamp Medium for Food Hygiene test: Staphylococcus aureus)
Code
06757
06756
Form
Package
Storage
Shelf Life
Cool
4 Months
30 Plates
Solid
100 Plates
forms small white or light blue colonies.
Small light blue matte flat colonies are not
Staphylococcus aureus.
Directions
Food Stamp is a prepared agar medium for
Stamp method, on which agar stands up
slightly above the rim of special Petri dish of
10 cm2. Take off the cap of Food Stamp and
gently press the medium against the
surface of specimen. The surface of agar is
elastic enough to be pressed firmly against
the specimen. Press against the different
parts of the specimen when several kinds of
Food Stamps are tested simultaneously.
Put the cap again immediately after
pressing.
Incubate at 35 - 37°C for 22 - 24 hours.
Storage
Keep dry at 4 – 10°C. Do not freeze.
Formula
Components
Peptone
Meat Extract
Lithium Chloride
Mannitol
Agar
Selective Agent
Chromogenic Substrate
Interpretations
Staphylococcus aureus forms blue (light
blue) lenticular wet grazed colonies, and
Coagulase-negative staphylococcus (CNS)
In 1 L
14.0 g
3.0 g
5.0 g
10.0 g
14.0 g
0.7 g
0.2 g
pH 7.4 ± 0.1
Back to the Index
15-9
06757-20100303 Koh SAI
Food Stamp “Nissui”
Sabouraud Agar (SABO)
(Simple and Easy Stamp Medium for Food Hygiene test: Bacillus cereus)
Code
06064
06063
Form
Package
Storage
Shelf Life
Cool
6 Months
30 Plates
Solid
100 Plates
All characteristic colonies should be
counted for evaluation.
Directions
Food Stamp is a prepared agar medium for
Stamp method, on which agar stands up
slightly above the rim of special Petri dish of
10 cm2 . Take off the cap of Food Stamp
and gently press the medium against the
surface of specimen. The surface of agar is
elastic enough to be pressed firmly against
the specimen. Press against the different
parts of the specimen when several kinds of
Food Stamps are tested simultaneously.
Put the cap again immediately after
pressing.
Incubate at 20-25°C for 2-5 days.
Storage
Keep dry at 4 – 10°C. Do not freeze.
Formula
Components
In 65.0 g/L
Peptone
10.5 g
Dextrose
40.0 g
Agar
14.5 g
pH 5.6 ± 0.2
Interpretations
Back to the Index
Fungi develop characteristic fluffy colonies
on the surface.
15-10
06064-20100303 Koh SAI
Food Stamp “Nissui”
Potato dextrose Agar with Chloramphenicol (PDA)
(Simple and Easy Stamp Medium for Food Hygiene test: Food poisoning Fungi)
Code
06755
06754
Form
Package
Storage
Shelf Life
Cool
5 Months
30 Plates
Solid
100 Plates
colonies can be counted for Fungi.
Directions
Food Stamp is a prepared agar medium for
Stamp method, on which agar stands up
slightly above the rim of special Petri dish of
10 cm2 . Take off the cap of Food Stamp
and gently press the medium against the
surface of specimen. The surface of agar is
elastic enough to be pressed firmly against
the specimen. Press against the different
parts of the specimen when several kinds of
Food Stamps are tested simultaneously.
Put the cap again immediately after
pressing.
Incubate at 20-25°C for 2-5 days.
Storage
Keep dry at 4 – 10°C. Do not freeze.
Formula
Components
In 39.1 g/L
Potato Extract
3.9 g
Dextrose
21.0 g
Chloramphenicol
100 mg
Agar
14.1 g
pH 5.6 ± 0.2
Interpretations
Since Chloramphenicol in the medium
inhibits the growths of other bacteria, all
Back to the Index
15-11
06755-20100303 Koh SAI
Food Plate X “Nissui”
(Quick Microbiological Test for Liquid Foods)
Code
Form
Package
Storage
Shelf Life
06231
Solid
10 Plates
Cool
5 Months
Directions
Take out a plate from the container, and dip
the plate into liquid specimen at length.
Take up the plate from the specimen, and
bring back to the container after excess
specimen is eliminated.
Incubate at 37°C for 24 hours.
1.Viable Bacterial count: Estimate a viable
bacterial count/ml by comparing its density
of all colonies grown on the surface of
modified BHI medium with a control chart.
2.Coliform group: Estimate Coliform
group/ml by comparing its density of blue
colonies grown on the surface of modified
X-GAL medium with a control chart.
Back to the Index
15-12
06231-20100304 Koh SAI
Compact Dry “Nissui” TC
(Simple and Easy Dry Medium for Microbial Count: Total Viable Bacterial Count)
Link to User's Manual
English
Traditional Chinese
Simplified Chinese
Code
06740
06741
Form
Package
Storage
Shelf Life
RT
18 Months
40 Plates
Dried
240 Plates
colonies eventually are appeared on the
plate but all plate sheets becomes
seemingly colored.
Put the cap again immediately after
inoculation.
Incubate at 35±1°C for 48±3 hours.
Directions
Compact Dry is ready to use and portable
plate containing dried sheet medium.
Detach necessary number of plate from a
set of four by bending up and down while
pressing the lid. It is available for solid food
staff (homogenize with buffer solution),
water or liquid sample and swab test. Drop
1 mL of specimen in the middle of dry sheet
of Compact Dry (except Compact Dry SL).
Inoculated sample diffuses automatically
and evenly into the plate (dried sheet
medium is being gel form).
Specimen should be diluted by buffer
solution to the level of concentration of less
than 300 cfu/plate. If bacteria more than
104 cfu were inoculated on a plate, no
colonies are formed, and no colored
Interpretations
The medium consists of non-selective
medium and redox indicator of 2,3,5Tripenyl Tetrazolium Chloride (TTC). Colonies
grown on Compact Dry TC are almost all red
colored.
Since some microorganism may not reduce
TTC to develop Red / Pink color, Compact
Dry TC may develop colonies that are not
necessarily clear red color.
16-1
Back to the Index
06740-20100304 Koh SAI
Compact Dry “Nissui” CF
(Simple and Easy Dry Medium for Microbial Count: Coliforms)
Link to User's Manual
English
Traditional Chinese
Simplified Chinese
Code
06744
06745
Form
Package
Storage
Shelf Life
RT
18 Months
40 Plates
Dried
240 Plates
colonies eventually are appeared on the
plate but all plate sheets becomes
seemingly colored.
Put the cap again immediately after
inoculation.
Incubate at 35±1°C for 24±3 hours.
Directions
Compact Dry is ready to use and portable
plate containing dried sheet medium.
Detach necessary number of plate from a
set of four by bending up and down while
pressing the lid. It is available for solid food
staff (homogenize with buffer solution),
water or liquid sample and swab test. Drop
1 mL of specimen in the middle of dry sheet
of Compact Dry (except Compact Dry SL).
Inoculated sample diffuses automatically
and evenly into the plate (dried sheet
medium is transforming to gel form).
Specimen should be diluted by buffer
solution to the level of concentration of less
than 300 cfu/plate. If bacteria more than
104 cfu were inoculated on a plate, no
colonies are formed, and no colored
Interpretations
Coliforms grow to develop Blue / Blue green
colonies as the medium contains
chromogenic enzyme substrate X-GAL.
Bacteria other than Coliforms are inhibited
to grow, and they do not form any colored
colonies even if they grow.
Back to the Index
16-2
06744-20100304 Koh SAI
Compact Dry “Nissui” EC
(Simple and Easy Dry Medium for Microbial Count: E. coli and Coliforms)
Link to User's Manual
English
Traditional Chinese
Simplified Chinese
Code
06742
06743
Form
Package
Storage
Shelf Life
RT
18 Months
40 Plates
Dried
240 Plates
colonies eventually are appeared on the
plate but all plate sheets become seemingly
colored.
Put the cap again immediately after
inoculation.
Incubate at 35±1°C for 24±3 hours.
Directions
Compact Dry is ready to use and portable
plate containing dried sheet medium.
Detach necessary number of plate from a
set of four by bending up and down while
pressing the lid. It is available for solid food
staff (homogenize with buffer solution),
water or liquid sample and swab test. Drop
1 mL of specimen in the middle of dry sheet
of Compact Dry (except Compact Dry SL).
Inoculated sample diffuses automatically
and evenly into the plate (dried sheet
medium is transforming to gel form).
Specimen should be diluted by buffer
solution to the level of concentration of less
than 300 cfu/plate. If bacteria more than
104 cfu were inoculated on a plate, no
colonies are formed, and no colored
Interpretations
Medium contains two kinds of chromogenic
enzyme substrate, Magenta-GAL and XGLUC.
Red / Pink color for Coliforms except for E.
coli while Blue / Blue purple color for E. coli
are observed respectively. Combined total
umber of both colonies of Red and Blue is
the total number of Coliform group.
Back to the Index
16-3
06742-20100304 Koh SAI
Compact Dry “Nissui” YM
(Simple and Easy Dry Medium for Microbial Count: Yeast and Mold)
Link to User's Manual
English
Traditional Chinese
Simplified Chinese
Code
06746
06747
Form
Package
Storage
Shelf Life
RT
18 Months
40 Plates
Dried
240 Plates
colonies eventually are appeared on the
plate but all plate sheets becomes
seemingly colored.
Put the cap again immediately after
inoculation.
Incubate at 20 - 25°C for 3 – 7 days.
Directions
Compact Dry is ready to use and portable
plate containing dried sheet medium.
Detach necessary number of plate from a
set of four by bending up and down while
pressing the lid. It is available for solid food
staff (homogenize with buffer solution),
water or liquid sample and swab test. Drop
1 mL of specimen in the middle of dry sheet
of Compact Dry (except Compact Dry SL).
Inoculated sample diffuses automatically
and evenly into the plate (dried sheet
medium is transforming to gel form).
Specimen should be diluted by buffer
solution to the level of concentration of less
than 300 cfu/plate. If bacteria more than
104 cfu were inoculated on a plate, no
colonies are formed, and no colored
Interpretations
The medium contains chromogenic enzyme
substrate X-Phos that develops Blue color
for many yeast, and antibiotics that inhibit
the growth of bacteria. Mold forms the
cottony colonies with characteristic color.
Some kinds of yeast do not develop Blue
color on Compact Dry YM.
Back to the Index
16-4
06746-20100304 Koh SAI
Compact Dry “Nissui” VP
(Simple and Easy Dry Medium for Microbial Count: Vibrio parahaemolyticus)
Link to User's Manual
English
Traditional Chinese
Simplified Chinese
Code
06746
06747
Form
Package
Storage
Shelf Life
RT
18 Months
40 Plates
Dried
240 Plates
colonies eventually are appeared on the
plate but all plate sheets becomes
seemingly colored.
Put the cap again immediately after
inoculation.
Incubate at 35±2°C for 18-20 hours.
Directions
Compact Dry is ready to use and portable
plate containing dried sheet medium.
Detach necessary number of plate from a
set of four by bending up and down while
pressing the lid. It is available for solid food
staff (homogenize with buffer solution),
water or liquid sample and swab test. Drop
1 mL of specimen in the middle of dry sheet
of Compact Dry (except Compact Dry SL).
Inoculated sample diffuses automatically
and evenly into the plate (dried sheet
medium is transforming to gel form).
Specimen should be diluted by buffer
solution to the level of concentration of less
than 300 cfu/plate. If bacteria more than
104 cfu were inoculated on a plate, no
colonies are formed, and no colored
Interpretations
The medium contains chromogenic enzyme
substrate X-Phos that develops Blue color
for many yeast, and antibiotics that inhibit
the growth of bacteria. Mold forms the
cottony colonies with characteristic color.
Some of yeast does not develop Blue color
on Compact Dry YM.
Back to the Index
16-5
06748-20100304 Koh SAI
Compact Dry “Nissui” X-SA
(Simple and Easy Dry Medium for Microbial Count: Staphylococcus aureus)
Link to User's Manual
English
Traditional Chinese
Simplified Chinese
Code
06729
06730
Form
Package
Storage
Shelf Life
RT
18 Months
40 Plates
Dried
240 Plates
colonies eventually are appeared on the
plate but all plate sheets becomes
seemingly colored.
Put the cap again immediately after
inoculation.
Incubate at 35±2°C for 24±2 hours.
Directions
Compact Dry is ready to use and portable
plate containing dried sheet medium.
Detach necessary number of plate from a
set of four by bending up and down while
pressing the lid. It is available for solid food
staff (homogenize with buffer solution),
water or liquid sample and swab test. Drop
1 mL of specimen in the middle of dry sheet
of Compact Dry (except Compact Dry SL).
Inoculated sample diffuses automatically
and evenly into the plate (dried sheet
medium is transforming to gel form).
Specimen should be diluted by buffer
solution to the level of concentration of less
than 300 cfu/plate. If bacteria more than
104 cfu were inoculated on a plate, no
colonies are formed, and no colored
Interpretations
Staphylococcus aureus generates light
blue/blue colonies. Some bacteria other
than Staphylococcus aureus may grow and
generate white colonies.
Back to the Index
16-6
06729-20100304 Koh SAI
Compact Dry “Nissui” SL
(Simple and Easy Dry Medium for Microbial Count: Salmonella)
Code
06732
06733
Form
Package
Storage
Shelf Life
RT
18 Months
40 Plates
Dried
240 Plates
Put the cap again immediately after inoculation.
Incubate at 41 - 43℃ for 20 - 24 hours.
Directions
Compact Dry is ready to use and portable plate
containing dried sheet medium. Detach necessary
number of plate from a set of four by bending up and
down while pressing the lid. It is available for solid
food staff (homogenize with buffer solution), water
or liquid sample and swab test. Drop 0.1mL of
enriched specimen at approx. 1 cm from the edge of
plate gently. After inoculating the specimen, drop
1mL of sterilized water at the opposite point where
specimen dropped. Water shall diffuse
automatically.
Inoculated sample diffuses automatically and evenly
into the plate (dried sheet medium is transforming to
gel form).
Specimen should be diluted by buffer solution to the
level of concentration of less than 300 cfu/plate. If
bacteria more than 104 cfu were inoculated on a
plate, no colonies are formed, and no colored
colonies eventually are appeared on the plate but all
plate sheets becomes seemingly colored.
Interpretations
<Salmonella Positive>
Black to Green isolated or fused colonies are
observed, and sheet around the colonies is changed
to Yellow If a large quantity of Salmonella is
inoculated on a plate, no isolated colonies are
formed (there may be several spots with fused Black
or Green colonies), but whole plate sheets become
seemingly Yellow.
<Salmonella Negative>
There is no color change occurred on the sheet. If it
were occurred, the sheet color would be changed to
Red or Reddish purple. No Black or Green colonies
are observed.
The sheet color might be changed to Yellow caused
by Pseudomonas or Proteus. But Yellow portion is
small and limited because of their less motility.
Back to the Index
16-7
06732-20100305 Koh SAI
Compact Dry “Nissui” X-BC
(Simple and Easy Dry Medium for Microbial Count: Staphylococcus aureus)
Link to User's Manual
English
Traditional Chinese
Simplified Chinese
Code
06727
06728
Form
Package
Storage
Shelf Life
RT
12 Months
40 Plates
Dried
240 Plates
colonies eventually are appeared on the
plate but all plate sheets becomes
seemingly colored.
Put the cap again immediately after
inoculation.
Incubate at 35±2°C for 24±2 hours.
Directions
Compact Dry is ready to use and portable
plate containing dried sheet medium.
Detach necessary number of plate from a
set of four by bending up and down while
pressing the lid. It is available for solid food
staff (homogenize with buffer solution),
water or liquid sample and swab test. Drop
1 mL of specimen in the middle of dry sheet
of Compact Dry (except Compact Dry SL).
Inoculated sample diffuses automatically
and evenly into the plate (dried sheet
medium is transforming to gel form).
Specimen should be diluted by buffer
solution to the level of concentration of less
than 300 cfu/plate. If bacteria more than
104 cfu were inoculated on a plate, no
colonies are formed, and no colored
Interpretations
Bacillus cereus generates green/blue
colonies. .
Back to the Index
16-8
06727-20100305 Koh SAI
Easy Wiping Kit “Nissui”
(Easy and convenient swab kit designed for Compact Dry)
Code
Form
Package
Storage
Shelf Life
06738
1 mL/tube
200 Tubes
RT
2 Years
solution may be inoculated direct in Compact
Dry without any dilution if number of bacteria
were suspected less than 103 cfu.
About 100 times specimen is easily prepared
by adding 2 drops (0.1 mL) of specimen in
Swab kit tube is dropped into 9 mL sterilized
diluting solution.
Direction for use
Unscrew the cap of tube, and pull out the
cotton swab, with which wipe a specific area
of surface of specimen material.
Drop back the cotton swab into the tube, and
screw tightly. Shake or upside-down the tube
well to mix for original specimen solution.
Unscrew the top-cap of the tube. By pressing
the middle of tube, squeeze all original
specimen solution (1 mL) out of the tube into
9 mL of sterilized diluting solution, which is
used as a 10 times diluted specimen for
inoculation.
If necessary, prepare further series of diluted
specimen with the sterilized diluting solution,
and inoculate 1 mL of specimen in the middle
of Compact Dry “Nissui”.
Formula
Tube contains sterilized Buffered Sodium
Chloride Peptone Solution (pH 7.0) ”Nissui”
Tube is so prepared that total volume
dropped is 1mL when all solution is squeezed
out.
Components
In 1 L
Peptone
1.0 g
Sodium Chloride
4.3 g
Potassium Dihydrogen
3.56 g
Phosphate
Disodium Phosphate
7.23 g
(anhydrous)
Warning
Do not touch the cotton swab with finger.
Since Compact Dry “Nissui” detects up to 103
cfu colonies in a plate, original specimen
16-9
Back to the Index
06738-20100308 Koh SAI
Sterilized Diluting Solution “Nissui”
Code
Package
01553
9mL × 4
01551
90mL
Storage
Shelf Life
RT
1 Year
4×8×4
20×2
Formula
In 1 L of
Distilled Water
Components
Sodium Chloride
Disodium Phosphate
Potassium Dihydrogen
Phosphate
9.0 g
0.0785 g
0.0127 g
Back to the Index
16-10
01553-20100308 Koh SAI
Opener with Sterilized Diluting Solution “Nissui”
Code
Form
Package
Storage
Shelf Life
06737
-
1
-
-
Back to the Index
16-11
06737-20100308 Koh SAI
Wiping Frame “Nissui”
Code
Form
Package
Storage
Shelf Life
06598
-
250
-
-
Back to the Index
16-12
06598-20100308 Koh SAI
Sterilized Spoid “Nissui”
Code
Form
Package
Storage
Shelf Life
06472
-
1000
-
-
Back to the Index
16-13
06472-20100308 Koh SAI
Clean Stamp “Nissui”
MSO Agar (MSO)
(Microbiological Test for Medical Ambience: MRSA)
Code
06781
06780
Form
Package
Storage
Shelf Life
Cool
2 Months
30 Plates
Solidified
100 Plates
Directions
Storage
Clean Stamp is a prepared agar medium for Stamp
method, on which agar stands up slightly above the rim of
special Petri dish of 10 cm2 . Take off the cap of Clean
Stamp and gently press the medium against the surface of
specimen. The surface of agar is elastic enough to be
pressed firmly against the specimen. Press against the
different parts of the specimen when several kinds of
Clean Stamps are tested simultaneously. Put the cap
again immediately after pressing.
Incubate at 35°C for 24 - 48 hours.
Do keep at 4 – 10°C. Do not freeze.
Formula
Components
Meat Extract
Peptone
Sodium Chloride
Mannitol
Sodium Pyruvate
Lithium Chloride
Oxacillin
Colistin
Amphotericin B
Aztreonam
Phenol Red
Egg Yolk
Agar
Interpretations
MRSA: MRSA forms round yellow/cream color colonies
with yellow surroundings around the colonies. Due to
positive Egg Yolk reaction, white turbidity is developed
inside the medium around the colonies while the surface
has pearly luster.
In case of faint Egg Yolk reaction is observed, extend
incubation to 48 hours, and read the reaction after
colonies are removed.
Non MRSA: Mannitol non-fermenting (no yellow color
change) and negative Egg Yolk reaction bacteria are
not MRSA.
17-1
In 1 L
1.0 g
10.0 g
40.0 g
10.0 g
2.0 g
5.0 g
6.0 mg
10.0 mg
2.0 mg
5.0 mg
25.0 mg
30.0 ml
15.0 g
pH 7.4 ± 0.1
Back to the Index
06781-20100308 Koh SAI
Clean Stamp “Nissui”
SCD Agar (SCD)
(Microbiological Test for Environment: Viable Bacterial Count Soybean Casein Digest Agar)
Code
06785
06784
Form
Package
Storage
Shelf Life
Cool
5 Months
30 Plates
Solidified
100 Plates
Directions
Storage
Clean Stamp is a prepared agar medium for
Stamp method, on which agar stands up
slightly above the rim of special Petri dish of
10 cm2 . Take off the cap of Clean Stamp
and gently press the medium against the
surface of specimen. The surface of agar is
elastic enough to be pressed firmly against
the specimen. Press against the different
parts of the specimen when several kinds of
Clean Stamps are tested simultaneously.
Put the cap again immediately after
pressing.
Incubate at 35°C for 24 - 48 hours.
Do keep at 4 – 10°C. Do not freeze.
Formula
Components
1L
Peptone
15.0 g
Soya Peptone
5.0 g
Sodium Chloride
5.0 g
Agar
15.0 g
pH 7.2 ± 0.1
Back to the Index
Interpretations
Count all colonies grown on the surface.
17-2
06785-20100308 Koh SAI
Clean Stamp “Nissui”
SCD Agar with Lecithin, Polysorbate 80 (SCDLP)
(Microbiological Test for Environment: Viable Bacterial Count Including inactivate agents)
Code
06787
06786
Form
Package
Storage
Shelf Life
Cool
5 Months
30 Plates
Solidified
100 Plates
into medium, more reliable results can be
obtained.
Incubate at 35°C for 24 - 48 hours.
Directions
Clean Stamp is a prepared agar medium for
Stamp method, on which agar stands up
slightly above the rim of special Petri dish of
10 cm2 . Take off the cap of Clean Stamp
and gently press the medium against the
surface of specimen. The surface of agar is
elastic enough to be pressed firmly against
the specimen. Press against the different
parts of the specimen when several kinds of
Clean Stamps are tested simultaneously.
Put the cap again immediately after
pressing.
This Clean Stamp is designed to measure
and detect the degree of pollution of the
specimen which surface has been
processed with chemicals or disinfectants.
As lecithin and polysorbate 80 inactivate
those chemicals and disinfectants absorbed
Interpretations
Count all colonies grown on the surface.
Storage
Do keep at 4 – 10°C. Do not freeze.
Formula
Components
Peptone
Soya Peptone
Sodium Chloride
Lecithin
Polysorbate 80
Agar
In 1 L
15.0 g
5.0 g
5.0 g
1.0 g
7.0 g
15.0 g
pH 7.2 ± 0.1
Back to the Index
17-3
06787-20100308 Koh SAI
Clean Stamp “Nissui”
Mannitol Salt Agar with Egg Yolk (MSEY)
(Microbiological Test for Medical Ambience: Staphylococcus)
Code
06783
06782
Form
Package
Storage
Shelf Life
Cool
5 Months
30 Plates
Solidified
100 Plates
reaction after colonies are removed.
Other Staphylococcus: Other Staphylococcus
forms small round white colonies with no color
changes or red surroundings around the
colonies, and do not show any positive Egg Yolk
reaction.
Directions
Clean Stamp is a prepared agar medium for
Stamp method, on which agar stands up slightly
above the rim of special Petri dish of 10 cm2 . Take
off the cap of Clean Stamp and gently press the
medium against the surface of specimen. The
surface of agar is elastic enough to be pressed
firmly against the specimen. Press against the
different parts of the specimen when several
kinds of Clean Stamps are tested simultaneously.
Put the cap again immediately after pressing.
Incubate at 35°C for 24 - 48 hours.
Storage
Do keep at 4 – 10°C. Do not freeze.
Formula
Components
Beef Extract
Peptone
Sodium Chloride
Mannitol
Phenol Red
Egg Yolk
Agar
Interpretations
Staphylococcus aureus: Staphylococcus aureus
forms round yellow/cream color colonies with
yellow surroundings around the colonies. Due to
positive Egg Yolk reaction, white turbidity is
developed inside the medium around the colonies
while the surface has pearly luster.
In case of faint Egg Yolk reaction is observed,
extend incubation to 48 hours, and read the
17-4
In 1 L
1.0 g
10.0 g
75.0 g
10.0 g
25.0 mg
30.0 ml
15.0 g
pH 7.4 ± 0.1
Back to the Index
06783-20100308 Koh SAI
Clean Stamp 25 “Nissui”
SCD Agar (SCD)
(Microbiological Test for Environment: Viable Bacterial Count, Soybean Casein Digest Agar)
Code
06791
06790
Form
Package
Storage
Shelf Life
Cool
5 Months
30 Plates
Solidified
150 Plates
of Code 05516 Trypto-Soya Agar “Nissui”
according to its directions, and aseptically
distributed and solidified in the special Petri
dishes having an inside diameter of 58 mm,
outside diameter of 73 mm and height of
18 mm. One clean film bag contains 3
Clean Stamp 25 s connected in series.
Directions
Clean Stamp 25 is a prepared agar medium
for Stamp method, on which agar stands up
slightly above the rim of special Petri dish of
25 cm2 . Take off the cap of Clean Stamp
25 and gently press the medium against
the surface of specimen. The surface of
agar is elastic enough to be pressed firmly
against the specimen. Press against the
different parts of the specimen when
several kinds of Clean Stamps are tested
simultaneously. Put the cap again
immediately after pressing.
Incubate at 30 - 35°C for 2 – 5 days.
Storage
Do keep at 4 – 10°C. Do not freeze.
Formula
Components
Peptone
Soya Peptone
Sodium Chloride
Agar
Interpretations
Count all colonies grown on the surface.
Remarks
In 40.0 g/L
15.0 g
5.0 g
5.0 g
15.0 g
pH 7.3 ± 0.2
Back to the Index
Clean Stamp 25 SCD is made and prepared
17-5
06791-20100309 Koh SAI
Clean Stamp “Nissui”
PSEU Agar (PSEU)
(Cetrimide Kanamycin sulfate Nalidixic Acid Agar,
Microbiological Test for Environment: Pseudomonas)
Code
Form
Package
Storage
Shelf Life
06789
Solidified
30 Plates
Cool
5 Months
Directions
Storage
Clean Stamp is a prepared agar medium for
Stamp method, on which agar stands up
slightly above the rim of special Petri dish of
10 cm2 . Take off the cap of Clean Stamp
and gently press the medium against the
surface of specimen. The surface of agar is
elastic enough to be pressed firmly against
the specimen. Press against the different
parts of the specimen when several kinds of
Clean Stamps are tested simultaneously.
Put the cap again immediately after
pressing.
Incubate at 35°C for 24 - 48 hours.
Do keep at 4 – 10°C. Do not freeze.
Formula
Components
Peptone
Magnesium Chloride
Potassium Sulfate
Cetrimide
Kanamycin Sulfate
Nalidixic acid
Glycerin
Agar
Interpretations
In 1 L
20.0 g
3.0 g
10.0 g
0.3 g
50.0 mg
5.0 mg
10.0 ml
15.0 g
pH 7.0 ± 0.1
Back to the Index
Pseudomonas develops round dome shape
or compressed colonies with yellowgreenish fluorescent on the medium.
17-6
06789-20100309 Koh SAI
Clean Stamp 25 “Nissui”
SCDLP Agar (SCDLP)
(Microbiological Test for Environment: Viable Bacterial Count Including inactivate agents)
Code
06793
06792
Form
Package
Storage
Shelf Life
Cool
5 Months
30 Plates
Solidified
150 Plates
05516 Trypto-Soya Agar “Nissui” plus Lecithin
and Polysorbate 80 according to its directions, and
aseptically distributed and solidified in the special Petri
dishes having an inside diameter of 58 mm, outside
diameter of 73 mm and height of 18 mm. One clean
film bag contains 3 Clean Stamp 25 s connected in
series.
Directions
Clean Stamp 25 SCDLP is a prepared agar medium for
Stamp method, on which agar stands up slightly above
the rim of special Petri dish of 25 cm2 . Take off the cap
of Clean Stamp 25 SCDLP and gently press the
medium against the surface of specimen. The surface
of agar is elastic enough to be pressed firmly against
the specimen. Press against the different parts of the
specimen when several kinds of Clean Stamps are
tested simultaneously. Put the cap again immediately
after pressing.
This Clean Stamp 25 SCDLP is designed to measure
and detect the degree of pollution of the specimen
which surface has been processed with chemicals or
disinfectants. As lecithin and polysorbate 80 inactivate
those chemicals and disinfectants absorbed into
medium, more reliable results can be obtained.
Incubate at 30 - 35°C for 2 – 5 days.
Storage
Do keep at 4 – 10°C. Do not freeze.
Formula
Components
Peptone
Soya Peptone
Sodium Chloride
Lecithin
Polysorbate 80
Agar
Interpretations
Count all colonies grown on the surface.
Remarks
In 1 L
15.0 g
5.0 g
5.0 g
1.0 g
7.0 g
15.0 g
pH 7.2 ± 0.1
Back to the Index
Clean Stamp 25 SCDLP is made and prepared of Code
17-7
06793-20100309 Koh SAI
Clean Stamp 25 “Nissui”
Sabouraud Agar with Chloramphenicol (CPSB)
(Microbiological Test for Environment: Fungus)
Code
06795
06794
Form
Package
Storage
Shelf Life
Cool
5 Months
30 Plates
Solidified
150 Plates
“Nissui” plus Chloramphenicol according to
its directions, and aseptically distributed
and solidified in the special Petri dishes
having an inside diameter of 58 mm,
outside diameter of 73 mm and height of
18 mm. One clean film bag contains 3
Clean Stamp 25 s connected in series.
Directions
Clean Stamp 25 CPSB is a prepared agar
medium for Stamp method, on which agar
stands up slightly above the rim of special
Petri dish of 25 cm2 . Take off the cap of
Clean Stamp 25 CPSB and gently press the
medium against the surface of specimen.
The surface of agar is elastic enough to be
pressed firmly against the specimen. Press
against the different parts of the specimen
when several kinds of Clean Stamps are
tested simultaneously. Put the cap again
immediately after pressing.
Incubate at 20 - 25°C for 2 – 5 days.
Storage
Do keep at 4 – 10°C. Do not freeze.
Formula
Components
Peptone
Dextrose
Chloramphenicol
Agar
Interpretations
Count all colonies grown on the surface.
Remarks
Clean Stamp 25 CPSB is made and
prepared of Code 05701 Sabouraud Agar
In 1 L
10.0 g
40.0 g
50 mg
15.0 g
pH 5.9 ± 0.2
Back to the Index
17-8
06795-20100309 Koh SAI
FASTKIT SLIM “Nissui”
B
C
A
Sample solution
drop section
Detecting section
Test
Control
(–)
(+)
Code
08630
08631
08632
08633
08634
EGG
MILK
WHEAT
BUCK
WHEAT
PEANUT
Package
Storage
Shelf Life
20 Tests
Cool
1 Year
Introduction
Operating Procedures for
Testing
The Food Sanitation Law obliges that 7 specified allergenic
ingredients (egg, milk, wheat, buckwheat, peanut, shrimp
and crab), which have a high risk of inducing food allergy,
should be listed on food labels. The Food Sanitation Law
also recommends that 18 ingredients, including soybeans,
should be listed on the label in a way similar to the
specified allergenic ingredients.
“Providing accurate information by listing of ingredients on
food labels” and “preventing contamination with the
ingredients which are not listed on the labels” are required
to prevent food allergy. Moreover, as a measure to prevent
contamination, routine management at the manufacturing
sites, including verification of the process of washing the
machines and devices used for production, is important.
(1) Leave the test strip in the aluminum pouch at room
temperature and remove from the pouch immediately
before use.
(2) With an oil-based marker pen, write the name of the
test sample or the number of the subject under test on the
absorbent pad of the test strip removed from the pouch.
(3) Place the test strip carefully on the flat stand and drop
a 100 μL-portion of the sample solution onto the sample
solution drop section. Otherwise, dispense a 150 μL
portion of the sample solution into a test tube and attach
the test strip to the test tube so that the sample solution
drop section of the test strip is immersed in the sample
solution.
Product Features
Formula
1) The simple one-step operation enables easy
assessment, and anybody can perform the test easily.
2) Because there is no need for special test equipment
and the test gives rapid results (in 15 minutes), it is best
for routine management at the production sites.
Contents
A: Test Strip
B: Dilution Buffer
C: Extraction Buffer
(1/10 Concentration)
18-1
In 1 Kit
2 Tests × 10 Packs
50 mL × 1 Bottle
100 mL × 1 Bottle
Back to the Index
08630-20100421 Koh SAI
FASTKIT ELISA ver. II “Nissui”
B
C
H
K
E D
J
A
I
Code
08615
08616
08617
08618
08619
08606
EGG
MILK
WHEAT
BUCK
WHEAT
PEANUT
SOYBEAN
F
G
Package
Storage
Shelf Life
96 Tests
Cool
6 Months
The FASTKIT ELISA Version II can simultaneously identify
the presence of multiple proteins.
The FASTKIT ELISA Version II are widely applicable to
samples ranging from raw ingredients to processed foods.
Introduction
Amendments to Food Sanitation Law in Japan have
rendered it mandatory to label 5 specific raw food
materials, (Eggs, Milk, Wheat, Buckwheat and Peanuts),
identified to have a high possibility of triggering food
allergies when foods contain more than a predetermined
amount of µg/mL or µg/g of the specified raw ingredients,
as of April 2002. In addition to clearly labeling the fore
mentioned food products, screening of such products is
becoming increasingly important in order to confirm the
amount of proteins into contained in the raw ingredient or
confirm the existence by a result of contamination due to
the mixing of these raw materials with other foodstuffs
and contamination on production lines.
FASTKIT ELISA Version II are the test kits for detecting
proteins in foodstuffs by using enzyme-linked
immunosorbent assay (ELISA).
Formula
Contents
In 1 Kit
A: Antibody immobilized plate
B: Standard solution (50 ng/mL)
C: Dilution buffer
1.8 mL × 1
100 mL × 1
D: Biotin-labeled antibody
E: Enzyme (peroxidase)-labeled
streptavidin
F: Chromogenic substrate (TMB)
G: Stop solution
Product Features
96 Wells × 1
150 μL × 1
150 μL × 1
12 mL × 1
12 mL × 1
H: Concentrated washing solution
100 mL × 1
I: Extraction reagent ①
50 mL × 1
100 mL × 1
50 mL × 1
J: Extraction reagent for soybean ②
K: Extraction reagent ③
The FASTKIT ELISA Version II are able to accurately
measure the amount of proteins in foods.
18-2
Back to the Index
08615-20100422 Koh SAI
FA test
EIA-Crustacea “Nissui”
4
3
2
5
FA test
Extraction Solution “Nissui”
6
A
7
B
C
2
1
Code
08621
08622
Form
Package
Storage
96 Tests
-
Shelf Life
1 Year
Cool
-
6 Months
FA test EIA-Crustacea “Nissui” is a high specific assay
system due to a high specific monoclonal antibody.
FA test EIA-Crustacea “Nissui” can cover a wide
measurement range of 0.3 to 20 ppm.
FA test EIA-Crustacea “Nissui” is widely applicable to
samples ranging from raw ingredients to processed foods.
Introduction
Amendments to Food Sanitation Law in Japan have
rendered it mandatory to label 5 specific raw food
materials, (Eggs, Milk, Wheat, Buckwheat and Peanuts),
identified to have a high possibility of triggering food
allergies when foods contain more than a predetermined
amount of µg/mL or µg/g of the specified raw ingredients,
as of April 2002. Similarly, shrimps, prawns (except
lobsters and crawfishes) and crabs is one of 20 raw food
materials recommended to label, conforming to Food
Sanitation Law. In addition to clearly labeling the abovementioned food products, screening of such products is
increasingly becoming important in order to confirm the
amount of proteins contained in the raw ingredient, or in
order to confirm the content due to the mixing of these
raw materials with other foodstuffs and contamination on
production lines.
FA test EIA-Crustacea “Nissui” is a test kit to determine
crustacean-derived proteins in foodstuffs precisely, based
on enzyme-linked immunosorbent assay (EIA) using a
crustacean tropomyosin-specific monoclonal antibody.
Formula
In 1 Kit
EIA-Crustacea Contents (08621)
1: Antibody fixed micro titer plate
2: Standard solution (50 ng/mL)
3: Dilution buffer
1 Plate (96 wells)
1.2 mL × 2 Vials
100 mL × 1 Bottle
4: Enzyme-labeled antibody
13 mL × 1 Vial
5: Enzyme-substrate solution
13 mL × 1 Vial
6: Stopping solution (1N H 2SO4)
13 mL × 1 Vial
7: Washing Solution (×10)
100 mL × 1 Bottle
In 1 Kit
Extraction Solution Components (08622)
Product Features
A: Extraction solution A (×20)
55 mL × 1 Bottle
B: Extraction solution B (×20)
55 mL × 1 Bottle
C: Extraction solution C (×20)
50 mL × 1 Bottle
Back to the Index
FA test EIA-Crustacea “Nissui” can determine crustaceanderived proteins in foodstuffs precisely.
18-3
08621-20100423 Koh SAI
FA test Immunochromato-Crustacea “Nissui”
1
2
3
Interpretation area
(Control Test)
Sample dropper area
(–)
(+)
Code
Form
Package
Storage
Shelf Life
08623
-
20 Tests
Cool
1 Year
simple operation and results obtainable in a
short period of time.
Introduction
Amendments to Food Sanitation Law in Japan
have rendered it mandatory to label 5 specific
raw food materials, (Eggs, Milk, Wheat,
Buckwheat and Peanuts), identified to have a
high possibility of triggering food allergies when
foods contain more than a predetermined
amount of µg/mL or µg/g of the specified raw
ingredients, as of April 2002. Similarly, as of
June 2008, shrimps, prawns (except lobsters
and crawfishes) and crabs are added to Food
Sanitation Law. In addition to clearly labeling
the above-mentioned food products, screening
of such products is increasingly becoming
important in order to confirm the amount of
proteins contained in the raw ingredient, or in
order to confirm the content due to the mixing
of these raw materials with other foodstuffs
and contamination on production lines.
FA test Immunochromato-Crustacea is a
detection kit for crustacean-derived protein in
foodstuffs, based on a lateral flow system, with
Product Features
FA test can detect crustacean-derived
tropomyosin in foodstuffs.
FA test is a high specific assay system due to a
high specific monoclonal antibody.
FA test can detect crustacean-derived
tropomyosin in foodstuffs with simple and
rapid operation.
FA test is widely applicable to samples ranging
from raw ingredients to processed foods.
Formula
Contents
1: Test device
2: Condensed extraction buffer
(10-fold condensate)
3: Sample dilution buffer
In 1 Kit
20 Tests
100 mL × 1 Bottle
100 mL × 1 Bottle
Back to the Index
18-13
08623-20100413 Koh SAI
Eagle’s MEM “Nissui” ①
(With Kanamycin, Phenol Red, Without L-Glutamine and Sodium Bicarbonate, Autoclavable)
Code
05900
08160
Form
Package
Storage
Shelf Life
100 g
Powder
1 Year
Cool
9.4 g × 10 Packs
Directions
2 Years
Formula
Dissolve 9.4 g of the medium in distilled water and
adjust the volume to 1,000 mL. Sterilize by
autoclaving at 121 centigrade for 15 minutes. Cool
the sterilized medium to room temperature and add
a proper amount of sterile 10% sodium bicarbonate
solution. (pH will be 7.1 – 7.4 at 37 centigrade in an
atmosphere of 5% CO2 in air by adding 12.5 – 22.0
mL of sodium bicarbonate solution) This mixture
should be stopper tightly and stored in a cool, dark
place. At the time of use, aseptically add 0.292 g of
L-glutamine (sterilized) and the desired amount of
serum.
Components
Sodium Chloride
Potassium Chloride
Calcium Chloride
Magnesium Sulfate
Sodium Dihydrogen Phosphate
Glucose
L-Arginine Hydrochloride
L-Cystine Dihydrochloride, H 2O
L-Tyrosine
Summary
L-Histidine Hydrochloride, H2O
L-Isoleucine
L-Leucine
L-Lysine Hydrochloride
L-Methionine
L-Phenylalanine
L-Threonine
L-Tryptophan
L-Valine
Succinic Acid
Eagle’s MEM “Nissui” 1 is a powdered medium
prepared according to prescription published by
Harry Eagle in 1959. Up to now, such media were
not autoclavable because of decomposition or
deterioration of the constituents, so that sterilization
was performed by filtration using a membrane filter.
Nissui Pharmaceutical Co., Ltd. has succeeded in
preparing an autoclavable powder medium. This
medium is specially manufactured so that
constituents of the medium and growth of cells with
this medium are not impaired. This medium has a
growth-promoting effect for cells such as HeLa or L
and other cell lines.
Sodium Succinate, 6H2O
Choline Bitartrate
Folic Acid
Inositol
Nicotinamide
Calcium Pantothenate
Pyridoxal Hydrochloride
Riboflavin
Thiamin Hydrochloride
Biotin
Kanamycin
Phenol Red
Storage and Expiration
Close tightly and store at 2-5°C in a dark and dry
place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date. Quality
of the product is not warranted after its shelf life.
When if medium or reagent touched eyes or mouth,
immediately wash with plenty of water, and consult
a physician.
Any medium, reagent and materials must be
sterilized by autoclaving or boiling water after use,
and then disposed as industrial waste according to
the law or regulations related to dispose.
In 9.4 g/L
6,800.0 mg
400.0 mg
200.0 mg
93.5 mg
115.0 mg
1,000.0 mg
126.0 mg
31.4 mg
36.0 mg
42.0 mg
52.0 mg
52.0 mg
73.0 mg
15.0 mg
32.0 mg
48.0 mg
10.0 mg
46.0 mg
75.0 mg
100.0 mg
1.8 mg
1.0 mg
2.0 mg
1.0 mg
1.0 mg
1.0 mg
0.1 mg
1.0 mg
0.02 mg
60.0 mg (Titer)
6.0 mg
Back to the Index
19-1
05900-20100310 Koh SAI
Eagle’s MEM “Nissui” ②
(With Kanamycin, Without Phenol Red, L-Glutamine and Sodium Bicarbonate, Autoclavable)
Code
Form
Package
Storage
Shelf Life
05901
Powder
100 g
Cool
1 Year
Directions
Formula
Dissolve 9.4 g of the medium in distilled water
and adjust the volume to 1,000 mL. Sterilize by
autoclaving at 121 centigrade for 15 minutes.
Cool the sterilized medium to room temperature
and add a proper amount of sterile 10% sodium
bicarbonate solution. (pH will be 7.1 – 7.4 at 37
centigrade in an atmosphere of 5% CO2 in air by
adding 12.5 – 22.0 mL of sodium bicarbonate
solution) This mixture should be stopper tightly
and stored in a cool, dark place. At the time of
use, aseptically add 0.292 g of L-glutamine
(sterilized) and the desired amount of serum.
Components
Sodium Chloride
Potassium Chloride
Calcium Chloride
Magnesium Sulfate
Sodium Dihydrogen
Phosphate
Glucose
L-Arginine Hydrochloride
In 9.4 g/L
6,800.0 mg
400.0 mg
200.0 mg
93.5 mg
115.0 mg
1,000.0 mg
126.0 mg
L-Cystine
Dihydrochloride, H2O
L-Tyrosine
L-Histidine
Hydrochloride, H2O
L-Isoleucine
L-Leucine
L-Lysine Hydrochloride
L-Methionine
L-Phenylalanine
L-Threonine
L-Tryptophan
L-Valine
Succinic Acid
Summary
Eagle’s MEM “Nissui” 2 is a powdered medium
prepared according to prescription published by
Harry Eagle in 1959. Up to now, such media were
not autoclavable because of decomposition or
deterioration of the constituents, so that
sterilization was performed by filtration using a
membrane filter.
Nissui Pharmaceutical Co., Ltd. has succeeded in
preparing an autoclavable-powdered medium. This
medium is specially manufactured so that
constituents of the medium and growth of cells
with this medium are not impaired. This medium
has a growth-promoting effect for cells such as
HeLa or L and other cell lines
31.4 mg
36.0 mg
42.0 mg
52.0 mg
52.0 mg
73.0 mg
15.0 mg
32.0 mg
48.0 mg
10.0 mg
46.0 mg
75.0 mg
100.0 mg
1.8 mg
1.0 mg
2.0 mg
1.0 mg
1.0 mg
1.0 mg
0.1 mg
1.0 mg
0.02 mg
Sodium Succinate, 6H2O
Storage and Expiration
Choline Bitartrate
Folic Acid
Inositol
Nicotinamide
Calcium Pantothenate
Pyridoxal Hydrochloride
Riboflavin
Thiamin Hydrochloride
Biotin
Kanamycin
Close tightly and store at 2-5°C in a dark and dry
place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after its
shelf life.
When if medium or reagent touched eyes or
mouth, immediately wash with plenty of water,
and consult a physician.
Any medium, reagent and materials must be
sterilized by autoclaving or boiling water after use,
and then disposed as industrial waste according to
the law or regulations related to dispose.
60.0 mg (Titer)
Back to the Index
19-2
05901-20100310 Koh SAI
Eagle’s MEM “Nissui” ③
(Without Kanamycin, Phenol Red, L-Glutamine and Sodium Bicarbonate, Autoclavable)
Code
Form
Package
Storage
Shelf Life
05902
Powder
100 g
Cool
1 Year
Directions
Formula
Dissolve 9.4 g of the medium in distilled water
and adjust the volume to 1,000 mL. Sterilize by
autoclaving at 121 centigrade for 15 minutes.
Cool the sterilized medium to room temperature
and add a proper amount of sterile 10% sodium
bicarbonate solution. (pH will be 7.1 – 7.4 at 37
centigrade in an atmosphere of 5% CO2 in air by
adding 12.5 – 22.0 mL of sodium bicarbonate
solution) This mixture should be stopper tightly
and stored in a cool, dark place. At the time of
use, aseptically add 0.292 g of L-glutamine
(sterilized) and the desired amount of serum.
Components
Sodium Chloride
Potassium Chloride
Calcium Chloride
Magnesium Sulfate
Sodium Dihydrogen
Phosphate
Glucose
L-Arginine Hydrochloride
In 9.4 g/L
6,800.0 mg
400.0 mg
200.0 mg
93.5 mg
115.0 mg
1,000.0 mg
126.0 mg
L-Cystine
Dihydrochloride, H2O
L-Tyrosine
L-Histidine
Hydrochloride, H2O
L-Isoleucine
L-Leucine
L-Lysine Hydrochloride
L-Methionine
L-Phenylalanine
L-Threonine
L-Tryptophan
L-Valine
Succinic Acid
Summary
Eagle’s MEM “Nissui” 3 is a powdered medium
prepared according to prescription published by
Harry Eagle in 1959. Up to now, such media were
not autoclavable because of decomposition or
deterioration of the constituents, so that
sterilization was performed by filtration using a
membrane filter.
Nissui Pharmaceutical Co., Ltd. has succeeded in
preparing an autoclavable-powdered medium. This
medium is specially manufactured so that
constituents of the medium and growth of cells
with this medium are not impaired. This medium
has a growth-promoting effect for cells such as
HeLa or L and other cell lines
Sodium Succinate, 6H2O
Storage and Expiration
Choline Bitartrate
Folic Acid
Inositol
Nicotinamide
Calcium Pantothenate
Pyridoxal Hydrochloride
Riboflavin
Thiamin Hydrochloride
Biotin
Close tightly and store at 2-5°C in a dark and dry
place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after its
shelf life.
When if medium or reagent touched eyes or
mouth, immediately wash with plenty of water,
and consult a physician.
Any medium, reagent and materials must be
sterilized by autoclaving or boiling water after use,
and then disposed as industrial waste according to
the law or regulations related to dispose.
31.4 mg
36.0 mg
42.0 mg
52.0 mg
52.0 mg
73.0 mg
15.0 mg
32.0 mg
48.0 mg
10.0 mg
46.0 mg
75.0 mg
100.0 mg
1.8 mg
1.0 mg
2.0 mg
1.0 mg
1.0 mg
1.0 mg
0.1 mg
1.0 mg
0.02 mg
Back to the Index
19-3
05902-20100311 Koh SAI
Eagle’s MEM Amino Acids and Vitamins Medium “Nissui”
Code
Form
Package
Storage
Shelf Life
05904
Powder
20 g
Cool
1 Year
Directions
Formula
Dissolve 0.88 g of the medium in distilled
water and adjust the volume to 1,000 mL.
When preparing 2 – 5 fold concentrated
solution, heat to 37°C and stir to dissolve.
After dissolving completely, the medium is
sterilized by filtration.
Components
L-Arginine
Hydrochloride
L-Cystine
Dihydrochloride
L-Cystine Hydrochloride,
H2 O
L-Histidine
Hydrochloride, H2O
L-Isoleucine
L-Leucine
L-Lysine Hydrochloride
L-Methionine
L-Phenylalanine
L-Threonine
L-Tryptophan
L-Tyrosine, Disodium
Salt
L-Valine
L-Glutamine
Thiamin Hydrochloride
Riboflavin
Pyridoxal Hydrochloride
Nicotinamide
Calcium Pantothenate
Folic Acid
Inositol
Choline Bitartrate
Summary
Eagle’s MEM Amino Acids and Vitamins
Medium “Nissui” is a powdered medium
excluding inorganic salts and glucose
prepared according to the minimum
essential medium (MEM) published by Harry
Eagle in 1959. This medium is used as a 25 fold concentrated solution for use with
small number cell cultures, suspension
cultures, cell colony formation by semisolid
agar, plaque formation and others.
Storage and Expiration
Close tightly and store at 2-5°C in a dark
and dry place. May be stored up to one (1)
year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after
its shelf life.
When if medium or reagent touched eyes or
mouth, immediately wash with plenty of
water, and consult a physician.
Any medium, reagent and materials must
be sterilized by autoclaving or boiling water
after use, and then disposed as
industrial/medical waste according to the
law or regulations related to dispose.
In 0.88 g/L
126.0 mg
15.66 mg
17.56 mg
42.0 mg
52.0 mg
52.0 mg
73.0 mg
15.0 mg
32.0 mg
48.0 mg
10.0 mg
44.75 mg
46.0 mg
292.0 mg
1.0 mg
0.1 mg
1.0 mg
1.0 mg
1.0 mg
1.0 mg
2.0 mg
1.8 mg
Back to the Index
19-4
05904-20100311 Koh SAI
Dulbecco’s PBS (-) “Nissui”
Code
Form
Package
05913
08190
Storage
Shelf Life
Cool
2 Years
100 g
Powder
08192
9.6 g × 10 Packs
96 g × 1 Pack
Directions
Formula
Dissolve 9.6 g of the medium in distilled
water and adjust the volume to 1,000 mL.
It can be sterilized by autoclaving (121°C,
15 minutes) or by membrane filtration.
Components
In 9.6 g/L
Sodium Chloride
8,000.0 mg
Potassium Chloride
Storage and Expiration
Disodium Phosphate
Close tightly and store at 2-5°C in a dark
and dry place. May be stored up to two (2)
year.
Monopotassium
Phosphate
200.0 mg
1,150.0 mg
200.0 mg
Back to the Index
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after
its shelf life.
When if medium or reagent touched eyes or
mouth, immediately wash with plenty of
water, and consult a physician.
Any medium, reagent and materials must
be sterilized by autoclaving or boiling water
after use, and then disposed as industrial
waste according to the law or regulations
related to dispose.
19-5
05913-20100311 Koh SAI
Glutamine “Nissui”
Code
Form
Package
Storage
Shelf Life
05908
Powder
0.3 g
Cool
2 Years
Directions
Add 10 mL of sterile distilled water and
dissolve completely. This solution is used as
a supplement to L-glutamine-free
autoclavable media, for example, Eagle’s
MEM “Nissui” 1.
Storage and Expiration
Store at 2-5°C in a dark and dry place. May
be stored up to two (2) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after
its shelf life.
When if medium or reagent touched eyes or
mouth, immediately wash with plenty of
water, and consult a physician.
Any medium, reagent and materials must
be sterilized by autoclaving or boiling water
after use, and then disposed as industrial
waste according to the law or regulations
related to dispose.
Formula (in 9.6 g for 1 liter)
1 vial contains 0.3 g sterile and lyophilized
L-glutamine.
Back to the Index
19-6
05908-20100311 Koh SAI
Dulbecco’s Modified Eagle Medium “Nissui” ①
(With L-Glutamine, Without Sodium Bicarbonate)
Code
Form
Package
Storage
Shelf Life
05915
Powder
100 g
Cool
1 Year
Directions
Formula
Dissolve 10.0 g of the medium in distilled water
and adjust the volume to 1,000 mL. Add a proper
amount of sodium bicarbonate. (pH will be 7.1 –
7.4 at 37, in an atmosphere of 5% CO2 in air by
adding 1.0 – 1.8 g sodium bicarbonate.) Dissolve
completely and immediately sterilize by filtration.
To avoid excessively raising the pH level of the
medium, it is advantageous to reduce the pH of
the medium by gassing with CO2 before filtration.
This mixture should be stopped tightly and stored
in a cool, dark place. At the time of use,
aseptically add the desired amount of serum.
Components
Sodium Chloride
Potassium Chloride
Calcium Chloride
Magnesium Sulfate
Sodium Dihydrogen
Phosphate, 2H2O
Ferric Nitrate, 9 H2O
Glucose
Sodium Pyruvate
L-Arginine Hydrochloride
L-Cystine Dihydrochloride
Summary
Dulbecco’s Modified Eagle Medium “Nissui” is a
powdered medium prepared on the basis of
research by R. Dulbecco et al. and is specially
manufactured. Compared with Eagle’s MEM, this
medium contains about twice the concentration of
amino acids and about four times the
concentration of vitamins. It contains Glycine and
L-serine, which are nonessential amino acids;
Pyruvate, which is important for glycolysis; and a
very small amount of ferric ion. It is nutritious for
the growth of cultured cells and is widely used for
research on viruses, primary cell cultures, and
single cell cultures.
Glycine
L-Histidine
Hydrochloride, H2O
L-Isoleucine
L-Leucine
L-Lysine Hydrochloride
L-Methionine
L-Phenylalanine
L-Serine
L-Threonine
L-Tryptophan
In 10.0 g/L
6,400.0 mg
400.0 mg
200.0 mg
97.7 mg
125.0 mg
0.1 mg
1,000.0 mg
110.0 mg
84.0 mg
62.6 mg
30.0 mg
L-Tyrosine, Disodium Salt
Storage and Expiration
L-Valine
Choline Bitartrate
Folic Acid
Nicotinamide
Calcium Pantothenate
Pyridoxal Hydrochloride
Riboflavin
Thiamin Hydrochloride
Inositol
Phenol Red
L-Glutamine
Close tightly and store at 2-5°C in a dark and dry
place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after its
shelf life.
When if medium or reagent touched eyes or
mouth, immediately wash with plenty of water,
and consult a physician.
Any medium, reagent and materials must be
sterilized by autoclaving or boiling water after use,
and then disposed as industrial waste according to
the law or regulations related to dispose.
42.0 mg
104.8 mg
104.8 mg
146.2 mg
30.0 mg
66.0 mg
42.0 mg
95.2 mg
16.0 mg
89.5 mg
93.6 mg
7.2 mg
4.0 mg
4.0 mg
4.0 mg
4.0 mg
0.4 mg
4.0 mg
7.2 mg
5.0 mg
584.0 mg
Back to the Index
19-7
05915-20100310 Koh SAI
Dulbecco’s Modified Eagle Medium “Nissui” ②
(Without L-Glutamine and Sodium Bicarbonate, Autoclavable)
Code
Form
Package
Storage
Shelf Life
05919
Powder
100 g
Cool
1 Year
Directions
Formula
Dissolve 10.0 g of the medium in distilled water and
adjust the volume to 1,000 mL. After stirring on a
magnetic stirrer for about 30 minutes, sterilize by
autoclaving at 121°C for 15 minutes. Cool the sterilized
medium to room temperature and add a roper amount
of sterile 10% sodium bicarbonate solution. (pH will be
7.1 – 7.4 at 37°C in an atmosphere of 5% CO2 in air by
adding 12-20 mL of sodium bicarbonate solution.) This
mixture should be stopped tightly and stored in a cool,
dark place. At the time of use, aseptically add 0.584 g
L-glutamine (sterilized) and the desired amount of
serum.
Components
Sodium Chloride
Potassium Chloride
Calcium Chloride
Magnesium Sulfate
Sodium
SodiumDihydrogen
Dihydrogen
Phosphate
Phosphate, 2H2O
Ferric Nitrate, 9 H2O
Glucose
Sodium Pyruvate
Succinic Acid
L-Arginine
Hydrochloride
Summary
Dulbecco’s Modified Eagle Medium “Nissui”② is a
powdered medium prepared according to research by
R. Dulbecco et al. Up to now, such media were not
autoclavable because of decomposition or deterioration
of the constituents, so that sterilization was
accomplished by filtration using a membrane filter.
Nissui Pharmaceutical Co., Ltd. Succeeded in preparing
an autoclavable powdered medium. This medium is
specially manufactured so that constituents of the
medium and growth of cells with this medium are not
impaired. Compared with Eagle’s MEM, this medium
contains about twice the concentration of amino acids
and about four times the concentration of vitamins. It
contains Glycine and L-serine, which are nonessential
amino acids; Pyruvate, which is important for glycolysis;
and a very small amount of ferric ion. It is nutritious for
the growth of cultured cells and is widely used for
research on viruses, primary cell cultures, and single
cell cultures.
Sodium
6 H 2O
L-CystineSuccinate,
Dihydrochloride
L-Arginine Hydrochloride
Glycine
L-Cystine Dihydrochloride
L-Histidine
Glycine
Hydrochloride, H2O
L-Histidine
L-Isoleucine
Hydrochloride,
H 2O
L-Leucine
L-Isoleucine
L-Lysine Hydrochloride
L-Leucine
L-Methionine
L-Lysine
Hydrochloride
L-Phenylalanine
L-Methionine
L-Serine
L-Phenylalanine
L-Threonine
L-Serine
L-Tryptophan
L-Threonine
L-Tyrosine, Disodium Salt
L-Tryptophan
L-Valine
L-Tyrosine,
Disodium Salt
Choline Bitartrate
L-Valine
Folic Acid
Choline
Bitartrate
Nicotinamide
Folic
AcidPantothenate
Calcium
Nicotinamide
Pyridoxal Hydrochloride
Calcium
Pantothenate
Riboflavin
Pyridoxal
Hydrochloride
Thiamin Hydrochloride
Riboflavin
Inositol
Thiamin
Hydrochloride
Phenol Red
Inositol
L-Glutamine
Phenol Red
Storage and Expiration
Close tightly and store at 2-5°C in a dark and dry place.
May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date. Quality of
the product is not warranted after its shelf life.
When if medium or reagent touched eyes or mouth,
immediately wash with plenty of water, and consult a
physician.
Any medium, reagent and materials must be sterilized
by autoclaving or boiling water after use, and then
disposed as industrial waste according to the law or
regulations related to dispose.
19-8
InIn10.0
9.5 g/L
6,400.0 mg
400.0 mg
200.0 mg
97.7 mg
108.0 mg
125.0
0.1 mg
1,000.0 mg
110.0 mg
106.0
84.0 mg
27.0 mg
62.6
84.0
30.0 mg
70.3 mg
42.0 mg
30.0 mg
104.8 mg
42.0 mg
104.8 mg
104.8 mg
146.2
104.8
30.0 mg
146.2
66.0 mg
30.0
42.0 mg
66.0
95.2 mg
42.0
16.0 mg
95.2
89.5 mg
16.0 mg
93.6
89.5
7.2 mg
93.6
4.0 mg
7.2 mg
4.0
4.0 mg
4.0 mg
4.0
0.4 mg
4.0 mg
0.4
7.2 mg
4.0
5.0 mg
7.2 mg
584.0
5.0 mg
Back to the Index
05919-20100310 Koh SAI
SFM-101 “Nissui”
Code
Package
Storage
Shelf Life
05963
Basal medium 12.5 g
Supplement A 10 mL (Lyophilized)
Supplement B 5 mL
Cool
1 Year
Directions
Formula
Basal Medium Components
Sodium Chloride
Potassium Chloride
Sodium Dihydrogen Phosphate, 2H 2O
Disodium Phosphate, 2H 2O
Calcium Chloride
Calcium Nitrate, 4H2O
Magnesium Sulfate
Sodium Selenite
L-Arginine
L-Arginine Hydrochloride
L-Asparagine, H2O
L-Asparatic Acid
L-Cysteine Hydrochloride, H 2O
L-Cysteine Dihydrochloride
L-Glutamic Acid
L-Glutamine
Glycine
L-Histidine
L-Histidine Hydrochloride, H2O
L-Hydroxyproline
L-Isoleucine
L-Leucine
L-Lysine Hydrochloride
L-Methionine
L-Phenylalanine
L-Proline
L-Serine
L-Threonine
L-Tyrosine
L-Tryptophan
L-Valine
p-Aminobenzonic Acid
Biotin
Choline Bitartrate
Choline Chloride
Folic Acid
Inositol
Nicotinamide
Calcium Pantothenate
Pyridoxal Hydrochloride
Pyridoxine Hydrochloride
Riboflavin
Thiamin Hydrochloride
Cyanocobalamin
Glucose
Sodium Pyruvate
Succnic Acid
Sodium Succinate
Hypozanthine
Thymidine
Glutathione
Putrescine Hydrochloride
Dihydroxyethilglycine
Phenol Red, Sodium Salt
Supplement A Components
Insulin (Bovine)
Transferrin
Supplement B Components
Monoethanolamine
Completely dissolve the entire contents of the
basal medium (12.5 g) in approximately 900 mL
of distilled water. Reconstitute Supplement A with
10 mL of distilled water, whereas Supplement B is
ready to use as is. Add both Supplement A and
Supplement B into the basal medium and add
sodium bicarbonate to the medium. If 1.0 – 1.5 g
of sodium bicarbonate is added to one liter of the
final medium, the pH of the medium at 37°C, 5%
CO2 will be 7.1 – 7.4. Add distilled water to bring
the total volume of the medium to 1,000 mL.
After thorough mixing, immediately sterilize the
final medium by membrane filtration. Keep
prepared medium at 2 - 10°C before use.
Storage and Expiration
Close tightly and store at 2-5 °C in a dark and dry
place. Shelf life of the product is one (1) year after
manufacturing.
Precautions
Use a low protein adsorption filter to prevent the
protein (Insulin and Transferrin) from being
adsorbed on the filter.
Store reconstituted Supplement A below -20°C, if
it its not used at once. However, avoid repeated
freezing and thawing.
Prepared medium should be used within two
months.
Summary
SFM-101 is a serum-free medium developed by
Nissui Pharmaceutical Co., Ltd. and is highly
effective for both cell growth and the production of
antibodies using mouse hybridomas. SFMeliminated the problems caused by differences in
batches of fetal calf serum, etc. And because it
contains a considerably lower concentration of
protein than media with serum, it allows easier
collection and purification of cell products
(monoclonal antibodies and physiologically active
substances).
In 12.5 g/L
6,000.0 mg
400.0 mg
75.0 mg
502.0 mg
100.0 mg
50.4 mg
71.0 mg
0.002 mg
100.0 mg
78.0 mg
43.4 mg
10.0 mg
15.7 mg
32.5 mg
10.0 mg
600.0 mg
10.0 mg
7.5 mg
21.0 mg
10.0 mg
51.0 mg
51.0 mg
56.5 mg
15.0 mg
23.5 mg
15.0 mg
30.0 mg
49.0 mg
28.0 mg
7.5 mg
48.0 mg
0.5 mg
0.2 mg
0.9 mg
26.5 mg
1.0 mg
18.5 mg
1.0 mg
0.6 mg
0.5 mg
0.5 mg
0.15 mg
1.0 mg
0.004 mg
2,000.0 mg
110.0 mg
37.5 mg
50.0 mg
0.025 mg
0.013 mg
0.5 mg
0.013 mg
1,800.0 mg
5.0 mg
In 10 mL/L
10.0 mg
10.0 mg
In 5 mL/L
20.0 mg
Back to the Index
19-9
05963-20100310 Koh SAI
RPMI 1640 Medium “Nissui” ①
(Without Sodium Bicarbonate)
Code
Form
Package
Storage
Shelf Life
05911
Powder
100 g
Cool
1 Year
Directions
Formula
Dissolve 10.4 g of the medium in distilled
water and adjust the volume to 1,000 mL.
When there is difficulty dissolving the
powder, the solution may be acidified to
about pH 6.0 with dry ice or CO2 gas. Add a
proper amount of sodium bicarbonate. (pH
will be 7.1 – 7.4 at 37°C, in an atmosphere
of 5% CO2 in air by adding 1.0 – 1.8 g
sodium bicarbonate.) Aseptically add the
desire amount of serum.
Components
L-Arginine
L-Asparagine, H2O
L-Asparatic Acid
L-Cystine Dihydrochloride
L-Glutamic Acid
Glutathione
Glycine
L-Histidine
L-Hydroxyproline
L-Isoleucine
L-Leucine
L-Lysine Hydrochloride
L-Methionine
L-Phenylalanine
L-Proline
L-Serine
L-Threonine
L-Tryptophan
L-Tyrosine
L-Valine
Biotin
Calcium Pantothenate
Choline Chloride
Folic Acid
Inositol
Nicotinamide
p-Aminobenzoic Acid
Pyridoxine Hydrochloride
Riboflavin
Thiamin Hydrochloride
Cyanocobalamin
Sodium Chloride
Potassium Chloride
Calcium Nitrate
Disodium Phosphate
Magnesium Sulfate
Glucose
L-Glutamine
Phenol Red
Summary
RPMI 1640 Medium “Nissui” 1 is a
powdered medium prepared according to
the prescription developed by George E.
Moore, former director of Roswell Park
Memorial Institute. This medium is
developed for suspension culture.
Storage and Expiration
Close tightly and store at 2-5°C in a dark
and dry place. May be stored up to one (1)
year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after
its shelf life.
When if medium or reagent touched eyes or
mouth, immediately wash with plenty of
water, and consult a physician.
Any medium, reagent and materials must
be sterilized by autoclaving or boiling water
after use, and then disposed as industrial
waste according to the law or regulations
related to dispose.
19-10
In 10.4 g/L
200.0 mg
56.8 mg
20.0 mg
65.0 mg
20.0 mg
1.0 mg
10.0 mg
15.0 mg
20.0 mg
50.0 mg
50.0 mg
40.0 mg
15.0 mg
15.0 mg
20.0 mg
30.0 mg
20.0 mg
5.0 mg
20.0 mg
20.0 mg
0.2 mg
0.25 mg
3.0 mg
1.0 mg
35.0 mg
1.0 mg
1.0 mg
1.0 mg
0.2 mg
1.0 mg
0.005 mg
6,000.0 mg
400.0 mg
69.5 mg
801.0 mg
48.8 mg
2,000.0 mg
300.0 mg
5.0 mg
Back to the Index
05915-20100310 Koh SAI
RPMI 1640 Medium “Nissui” ②
(Without L-Glutamine and Sodium Bicarbonate, Autoclavable)
Code
Form
Package
Storage
Shelf Life
05918
Powder
100 g
Cool
1 Year
Directions
Formula
Dissolve 10.2 g of the medium in distilled water
and adjust the volume to 1,000 mL. Sterilize by
autoclaving at 121°C for 15 minutes. Cool the
sterilized medium to room temperature and add a
proper amount of sterile 10% sodium bicarbonate.
(pH will be 7.1 – 7.4 at 37°C, in an atmosphere of
5% CO2 in air by adding 18 – 30 mL of sodium
bicarbonate solution.) Aseptically add 0.3 g Lglutamine and the desire amount or serum.
Components
L-Arginine Hydrochloride
L-Asparagine, H2O
L-Asparatic Acid
L-Cysteine Hydrochloride, H 2O
L-Glutamic Acid
Succinic Acid
Sodium Succinate, 6H2O
Glutathione
Glycine
Summary
RPMI 1640 is a medium for suspension culture
investigated by George E. Moore, former director
of Roswell Park Memorial Institute. Up to now,
such media were not autoclavable because of
decomposition or deterioration of the
constituents, so that sterilization had been
performed by filtration using a membrane filter.
RPMI 1640 Medium “Nissui” 2 is an autoclavablepowdered medium specially manufactured to
overcome the difficulties associated with filtersterilized media, such as contamination by
Mycoplasma. This medium has growth promoting
effect to primary cultures of mouse and human
leukemia cells, various kinds of cell lines and
KATO-III cells.
L-Histidine Hydrochloride, H2O
Storage and Expiration
Close tightly and store at 2-5°C in a dark and dry
place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after its
shelf life.
When if medium or reagent touched eyes or
mouth, immediately wash with plenty of water,
and consult a physician.
Any medium, reagent and materials must be
sterilized by autoclaving or boiling water after use,
and then disposed as industrial waste according to
the law or regulations related to dispose.
Back to the Index
19-11
L-Hydroxyproline
L-Isoleucine
L-Leucine
L-Lysine Hydrochloride
L-Methionine
L-Phenylalanine
L-Proline
L-Serine
L-Threonine
L-Tryptophan
L-Tyrosine
L-Valine
Biotin
Calcium Pantothenate
Choline Chloride
Folic Acid
Inositol
Nicotinamide
p-Aminobenzoic Acid
Pyridoxine Hydrochloride
Riboflavin
Thiamin Hydrochloride
Cyanocobalamin
Sodium Chloride
Potassium Chloride
Calcium Nitrate
Monosodium Phosphate
Magnesium Sulfate
Glucose
Phenol Red
In 10.2 g/L
240.0 mg
56.8 mg
20.0 mg
72.9 mg
20.0 mg
46.0 mg
164.0 mg
1.0 mg
10.0 mg
20.3 mg
20.0 mg
50.0 mg
50.0 mg
40.0 mg
15.0 mg
15.0 mg
20.0 mg
30.0 mg
20.0 mg
5.0 mg
20.0 mg
20.0 mg
0.2 mg
0.25 mg
3.0 mg
1.0 mg
35.0 mg
1.0 mg
1.0 mg
1.0 mg
0.2 mg
1.0 mg
0.005 mg
6,000.0 mg
400.0 mg
69.5 mg
677.0 mg
48.8 mg
2,000.0 mg
5.0 mg
05918-20100310 Koh SAI
ES Medium “Nissui”
(With Kanamycin, Without L-Glutamine and Sodium Bicarbonate, Autoclavable)
Code
Form
Package
Storage
Shelf Life
05971
Powder
100 g
Cool
1 Year
Directions
Formula
Components
Sodium Chloride
Potassium Chloride
Dissolve 9.7 g of the medium in distilled water
and adjust the volume to 1,000 mL. Sterilize by
autoclaving at 121°C for 15 minutes. Cool the
sterilized medium to room temperature and
add a proper amount of sterile 10% sodium
bicarbonate. (pH will be 7.1 – 7.4 at 37°C, in an
atmosphere of 5% CO2 in air by adding 12.5 –
22.0 mL of sodium bicarbonate solution.) This
mixture should be stopped tightly and store in a
cool dark place. At the time of use, aseptically
add 0.292 g L-glutamine (sterilized) and the
desired amount of serum.
Sodium Dihydrogen Phosphate
Magnesium Sulfate
Calcium Chloride
Glucose
L-Arginine Hydrochloride
L-Asparagine, H2O
L-Asparatic Acid
L-Alanine
L-Cysteine Hydrochloride, H 2O
L-Glutamic Acid
Glycine
L-Tyrosine
Summary
ES medium “Nissui” is a powdered medium
prepared on the basis of the prescription
published by H. Koyama et al. In this medium,
Eagle’s MEM is supplemented by seven amino
acids, sodium Pyruvate and Cyanocobalamin,
and the medium is autoclavable similar to
Eagle’ MEM “Nissui”.
L-Histidine Hydrochloride, H2O
L-Isoleucine
L-Leucine
L-Lysine Hydrochloride
L-Methionine
L-Phenylalanine
L-Proline
L-Threonine
L-Serine
L-Tryptophan
L-Valine
Succinic Acid
Sodium Succinate, 6 H2O
Choline Bitartrate
Folic Acid
Inositol
Nicotinamide
Calcium Pantothenate
Pyridoxine Hydrochloride
Riboflavin
Thiamin Hydrochloride
Biotin
Sodium Pyruvate
Cyanocobalamin
Kanamycin
Phenol Red
Storage and Expiration
Close tightly and store at 2-5 °C in a dark, dry
place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after its
shelf life.
When if medium or reagent touched eyes or
mouth, immediately wash with plenty of water,
and consult a physician.
Any medium, reagent and materials must be
sterilized by autoclaving or boiling water after
use, and then disposed as industrial waste
according to the law or regulations related to
dispose.
Back to the Index
In 9.7 g/L
6,800.0 mg
400.0 mg
115.0 mg
93.5 mg
200.0 mg
1,000.0 mg
126.0 mg
30.0 mg
27.0 mg
18.0 mg
31.4 mg
30.0 mg
15.0 mg
36.0 mg
42.0 mg
52.0 mg
52.0 mg
73.0 mg
15.0 mg
32.0 mg
23.0 mg
48.0 mg
21.0 mg
10.0 mg
46.0 mg
75.0 mg
100.0 mg
1.8 mg
1.0 mg
2.0 mg
1.0 mg
1.0 mg
1.0 mg
0.1 mg
1.0 mg
0.02 mg
110.0 mg
0.1 mg
60.0 mg (Titer)
6.0 mg
19-12
05971-20100312 Koh SAI
Medium 199 “Nissui”
(Without Sodium Bicarbonate)
Code
Form
Package
Storage
Shelf Life
05909
Powder
100 g
Cool
1 Year
Directions
Formula
Components
L-Arginine Hydrochloride
L-Histidine Hydrochloride, H2O
L-Lysine Hydrochloride
L-Tryptophan
L-Phenylalanine
L-Methionine
L-Serine
L-Threonine
L-Leucine
L-Isoleucine
L-Valine
L-Glutamic Acid
L-Aspartic Acid
L-Alanine
L-Proline
L-Hydroxyproline
Glycine
L-Glutamine
L-Cystine
L-Tyrosine
L-Cysteine Hydrochloride, H 2O
p-Aminobenzoic Acid
Biotin
Calcium Pantothenate
Folic Acid
Inositol
Nicotinic acid
Nicotinamide
Pyridoxal Hydrochloride
Pyridoxine Hydrochloride
Riboflavin
Thiamin Hydrochloride
Vitamin A
Ascorbic Acid
α-Tocopherol Disodium Phosphate
Calciferol
Menadione
Choline Bitartrate
D-2-Deoxyribose
Adenine
Guanine Hydrochloride
Hypoxanthine
Thymine
Uracil
Xanthin
Adenylic Acid
D-Ribose
Polysorbate 80
Cholesterol
Glutathione
Sodium Acetate
Adenosine Triphosphate, Disodium Salt
Glucose
Sodium Chloride
Potassium Chloride
Calcium Chloride
Magnesium Sulfate
Sodium Dihydrogen Phosphate
Ferric Nitrate, 9H2O
Phenol Red
Dissolve 9.5 g of the medium in distilled water
and adjust the volume to 1,000 mL. Don’t heat
over 37°C to dissolve. Add a proper amount of
sodium bicarbonate. (pH will be 7.1 – 7.4 at 37°C,
in an atmosphere of 5% CO2 in air by adding 1.3 –
2.2 g sodium bicarbonate.) Dissolve completely
and immediately sterilize by filtration. To avoid
excessively raising the pH level of the medium, it
is advantageous to reduce the pH of the medium
by gassing with CO2 before filtration. Aseptically
add the desire amount of serum.
Summary
Medium 199 “Nissui” is a powdered medium
prepared according to the synthetic medium
published by Morgan, Morton and Parker in 1950
and contains Earle’s BSS. This medium is one of
the earliest reported synthetic media and was first
developed to maintain chick embryo tissues. It
contains various kinds of amino acids, vitamins,
nucleic acid precursors and others. This medium
cannot increase cells but is utilized to maintain
cells in virus studies and to produce poliovaccine.
With the addition of serum, it is a superior
medium for primary cultures.
Storage and Expiration
Close tightly and store at 2-5°C in a dark, dry
place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after its
shelf life.
When if medium or reagent touched eyes or
mouth, immediately wash with plenty of water,
and consult a physician.
Any medium, reagent and materials must be
sterilized by autoclaving or boiling water after use,
and then disposed as industrial waste according to
the law or regulations related to dispose.
19-13
In 9.5 g/L
70.0 mg
21.9 mg
70.0 mg
10.0 mg
25.0 mg
15.0 mg
25.0 mg
30.0 mg
60.0 mg
20.0 mg
25.0 mg
66.8 mg
30.0 mg
25.0 mg
40.0 mg
10.0 mg
50.0 mg
100.0 mg
20.0 mg
40.0 mg
0.11 mg
0.05 mg
0.01 mg
0.01 mg
0.01 mg
0.05 mg
0.025 mg
0.025 mg
0.025 mg
0.025 mg
0.01 mg
0.01 mg
0.1 mg
0.05 mg
0.01 mg
0.1 mg
0.01 mg
0.9 mg
0.5 mg
10.0 mg
0.3 mg
0.3 mg
0.3 mg
0.3 mg
0.3 mg
0.2 mg
0.5 mg
5.0 mg
0.2 mg
0.05 mg
50.0 mg
1.0 mg
1,000.0 mg
6,800.0 mg
400.0 mg
200.0 mg
97.7 mg
102.9 mg
0.72 mg
6.0 mg
Back to the Index
05909-20100312 Koh SAI
Ham’s F12 Medium “Nissui”
(Without Sodium Bicarbonate)
Code
Form
Package
Storage
Shelf Life
05910
Powder
100 g
Cool
1 Year
Directions
Formula
Components
Sodium Chloride
Potassium Chloride
Disodium Phosphate
Copper Sulfate, 5H 2O
Zinc Sulfate, 7H2O
Ferrous Sulfate, 7H2O
Magnesium Chloride
Calcium Chloride
Glucose
L-Arginine Hydrochloride
L-Alanine
L-Asparagine, H2O
L-Aspartic Acid
L-Cysteine Hydrochloride, H 2O
L-Glutamic Acid
L-Glutamine
Glycine
L-Histidine Hydrochloride, H 2O
L-Isoleucine
L-Leucine
L-Lysine Hydrochloride
L-Methionine
L-Phenylalanine
L-Proline
L-Serine
L-Threonine
L-Tryptophan
L-Tyrosine
L-Valine
Biotin
Choline Chloride
Calcium Pantothenate
Folic Acid
Hypoxanthine
Inositol
Putrescine Dihydrochloride
Pyridoxine Hydrochloride
Riboflavin
Sodium Pyruvate
Thiamin Hydrochloride
Thymidine
Cyanocobalamin
Nicotinamide
Lipoic Acid
Linoleic Acid
Phenol Red
Dissolve 10.6 g of the medium in distilled water
and adjust the volume to 1,000 mL. Don’t heat
over 37°C to dissolve. Add a proper amount of
sodium bicarbonate. (pH will be 7.1 – 7.4 at
37°C, in an atmosphere of 5% CO2 in air by
adding 1.2 – 2.0 g sodium bicarbonate.)
Sterilize by filtration immediately. To avoid
excessively raising the pH level of the medium,
it is advantageous to reduce the pH of the
medium by gassing with CO2 before filtration.
Rehydrated medium will keep 1-2 months
when it is stored in a freezer. Aseptically add
the desire amount of serum.
Summary
Ham’s F12 Medium “Nissui” is a powdered
medium prepared according to the prescription
published by Richard G. Ham in 1965 and
made by special manufacturing. This medium
is recommended for proliferation of
mammalian cells as clones, especially, Chinese
Hamster cells. It is also recommended for
primary cultures.
Storage and Expiration
Close tightly and store at 2-5°C in a dark, dry
place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after its
shelf life.
When if medium or reagent touched eyes or
mouth, immediately wash with plenty of water,
and consult a physician.
Any medium, reagent and materials must be
sterilized by autoclaving or boiling water after
use, and then disposed as industrial waste
according to the law or regulations related to
dispose.
Back to the Index
In 10.6 g/L
7,599.0 mg
224.0 mg
142.0 mg
0.0025 mg
0.863 mg
0.834 mg
57.19 mg
33.31 mg
1,802.0 mg
211.0 mg
8.91 mg
15.01 mg
13.31 mg
35.12 mg
14.71 mg
146.0 mg
7.51 mg
20.96 mg
3.94 mg
13.12 mg
36.54 mg
4.48 mg
4.96 mg
34.53 mg
10.51 mg
11.91 mg
2.04 mg
5.44 mg
11.71 mg
0.0073 mg
14.0 mg
0.477 mg
1.32 mg
4.08 mg
18.0 mg
0.161 mg
0.062 mg
0.038 mg
110.0 mg
0.337 mg
0.727 mg
1.36 mg
0.037 mg
0.206 mg
0.084 mg
1.24 mg
19-14
05910-20100312 Koh SAI
Hanks’ Solution “Nissui” ①
(Without Sodium Bicarbonate)
Code
Form
Package
Storage
Shelf Life
05905
Powder
100 g
Cool
2 Years
Directions
Formula
Components
Sodium Chloride
Potassium Chloride
Disodium Phosphate
Potassium Dihydrogen
Phosphate
Magnesium Sulfate
Magnesium Chloride
Calcium Chloride
Glucose
Phenol Red
Dissolve 9.8 g of the medium in distilled water
and adjust the volume to 1,000 mL. After
dissolving completely, add a proper amount of
sodium bicarbonate. (pH will be 7.3 – 7.6 at
37°C, in an atmosphere of 5% CO2 in air by
adding 0.2 – 0.35 g sodium bicarbonate.)
Sterilize by filtration immediately. To avoid
excessively raising the pH level of the medium,
it is advantageous to reduce the pH of the
medium by gassing with CO2 before filtration.
Storage and Expiration
Close tightly and store at 2-5°C in a dark, dry
place. May be stored up to two (2) year.
In 9.8 g/L
8,000.0 mg
400.0 mg
47.9 mg
60.0 mg
48.8 mg
46.8 mg
140.0 mg
1,000.0 mg
6.0 mg
Hanks’ Solution “Nissui” ②
(Without Phenol Red and Sodium Bicarbonate)
Code
Form
Package
Storage
Shelf Life
05906
Powder
100 g
Cool
2 Years
Directions
Formula
Components
Sodium Chloride
Potassium Chloride
Disodium Phosphate
Potassium Dihydrogen
Phosphate
Magnesium Sulfate
Magnesium Chloride
Calcium Chloride
Glucose
Hanks’ Solution “Nissui” 2 is a powdered BSS prepared
with same composition as Hanks’ Solution “Nissui” 1
without phenol red and is used for dialysis, experiments of
immunology and others. Dissolve 9.8 g powders in distilled
water and adjust the volume to 1,000 mL. After dissolving
completely, add a proper amount of sodium bicarbonate.
(pH will be 7.3 – 7.6 at 37°C, in an atmosphere of 5% CO2
in air by adding 0.2 – 0.35 g sodium bicarbonate.) Sterilize
by filtration immediately. To avoid excessively raising the
pH level of the medium, it is advantageous to reduce the
pH of the medium by gassing with CO2 before filtration.
Storage and Expiration
Close tightly and store at 2-5 °C in a dark, dry place. May
be stored up to two (2) year.
19-15
In 9.8 g/L
8,000.0 mg
400.0 mg
47.9 mg
60.0 mg
48.8 mg
46.8 mg
140.0 mg
1,000.0 mg
Back to the Index
05905-20100312 Koh SAI
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