Cell Biology - Harvard Apparatus
Cell Biology
NEW• Nitric Oxide Measurement Systems ............see pages M2 – M5
NEW• Oxygen Measurement Systems ..................see pages M6 – M14
NEW
6-Channel Oxygen System Model 928,
see page M18
• Respirometry Systems ...............................see pages M15 – M22
NEW
• pH and Reference Electrodes ....................see pages M23 – M31
• Microelectrode Holders ..............................see pages M32 – M44
• Capillary Glass ..............................................see pages M45 – M49
• Micropipette Pullers....................................see pages M50 – M54
NEW• Electroporation Products ...........................see pages M55 – M61
NEW• Electroporation Electrodes
and Accessories ............................................see pages M62 – M69
NEW
MOS 25 and MOS 96 Advanced Electroporation
Systems, see page M55
NEW• Microinjection and Microperfusion
Systems...........................................................see pages M70 – M79
• Micro-Incubation Products ........................see pages M80 – M86
NEW• Chambers for 35mm Cell
Culture Dishes...............................................see pages M87 – M93
NEW• Special Chambers Systems.....................see pages M94 – M111
NEW• Chamber Platforms and Stage
Adapters.....................................................see pages M112 – M123
NEW
ZonaDrill™ Laser System, see page M74
NEW• Temperature Controllers.......................see pages M124 – M129
NEW• Temperature Controller
Peripherals................................................see pages M130 – M134
NEW• Perfusion Controllers.............................see pages M135 – M148
NEW• Microscopes and Illuminators .............see pages M149 – M159
NEW• Tissue Sampling Systems .....................see pages M160 – M165
NEW• Diffusion (Ussing) Chambers ...............see pages M166 – M175
NEW• Voltage/Current Clamps .......................see pages M176 – M185
NEW
NEW
SC-20 Dual In-Line Solution Heater/Cooler,
see page M130
NEW• Micromanipulation Products ...............see pages M186 – M201
• Cell Biology Books....................................................see page M202
Dovetail Slide Micromanipulators,
see page M191
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M1
Nitric Oxide
NEW
inNO-T Nitric Oxide Measurement System
Specialized Tools For Bioresearch
BS4 69-3054 Dual
Channel inNO-T System
BS4 69-3050
1 Channel inNO-T System
•
NEW Dual Channel System
•
Uses ‘amiNO’ sensors which are
up to 100 times more sensitive
than others on the market today
The ‘inNO-T’ system is the most technologically advanced nitric oxide electrochemical detection system on the market today.
• inNO-T system is the first available
NO system to have temperature
It combines a nitric oxide configured
compensation
potentiostat and a sophisticated software
• inNO-T system virtually eliminates
controlled data acquisition system in one
base line drift due to temperature
compact battery powered unit. When used
changes
with the ‘amiNO’ series of NO sensors, this
• Combination NO meter and data
unit provides an NO detection sensitivity
acquisition system
increase of up to 100 times that of any
• Battery-powered benchtop/portable
system (5 year battery life)
other electrochemical detection system on
the market today. Its state of the art bat• 24 bit A/D converter for the widest
dynamic range (16,000,000 to 1)
tery powered design allows the unit to run
continuously for up to 5 years, making it
• User friendly recording, playback
and analysis
ideal for any electrically isolated and/or
• Software compatible with
portable application.
Windows 95/98, 2000 and NT
The ‘inNO-T’ system is the first available
• Continuous recordings (over 1
nitric oxide system to include temperamonth)
ture compensation which further
• System shipped complete with NO
enhances the detection limits and data
meter, software, 2 types of NO
sensors, and calibration kit
reliability by virtually eliminating baseline drift caused by temperature
• Auto zero
changes. A temperature probe is supplied
• Computer chart recorder
with each system. Its internal 24 bit ana• RS-232 interface
log to digital converter and built in dig• Digital filter
ital notch filtering allows for accurate,
• Menu driven commands
noise free, detection of Nitric Oxide levels
of less than 0.01 nM. The 24 bit A/D technology also gives the unit a dynamic range in excess of 16,000,000 to 1. Its sophisticated control and analysis software can be linked to any computer system that utilizes
Windows™ 95/98, 2000 or NT via the ‘inNO’s’ internal RS-232 interface.
™
M2
inNO-T Nitric Oxide
Measurement System
‘amiNO’ Series of
Nitric Oxide Sensors
•
All NO sensors are combination
sensors, no external reference
electrode required
•
Mechanically rugged membrane
•
All sensors covered with proprietary
triplecoat gas permeable membrane
to guarantee selectivity and very fast
response time
•
All sensors completely shielded from
environmental/electrical noise
•
All sensors work the ‘inNO-T’ meter
as well as many other currently
available meters
•
One calibration procedure, simple,
easy, no expensive chemicals
Using the latest state of the art combination
NO sensor design, the ‘amiNO’ series of
nitric oxide sensors increases the sensitivity
of electrochemical nitric oxide measurement, by up to 100 times that of any existing
electrochemical sensor on the market today.
They are the first NO sensors to break the
100 pA/nM barrier, see chart on page M4.
This higher sensitivity greatly reduces any
measurement errors due to baseline drift
associated with temperature changes. This
baseline drift has been a particular concern
with older technology sensors that do not
have the enhanced sensitivity characteristics
of the ‘amiNO’ sensors. Additionally the new
‘amiNO’ series adds newly designed sensor
types for application involving in-vivo and
large surfaces (cultured cells). Customized
sensors are available upon request to meet
specialized applications, call Harvard
Apparatus Customer Service for details.
Harvard Apparatus offers 4 different Nitric
Oxide Measurement Systems. Each system
includes either a 1-channel or 2-channel
inNO-T Temperature compensated meter
plus an BS4 69-0012 amiNO-2000 sensor,
BS4 69-0016 SensoReady Electrode
Polorizer and a temperature probe (for a
complete listing of system components see
chart on page M4). Each system is offered in
either 115 or 230 VAC.
A gas phase NO sensor is
currently under development,
please call for details.
11,000 Specialty Products to Enhance Your Bioresearch
Nitric Oxide
NEW
inNO-T Nitric Oxide Measurement System
amiNO-7 Sensor
amiNO-IV Sensor
amiNO-2000 Sensor
• 7 µm tip diameter
• Flat 2 mm tip diameter
• 600 µm tip diameter
• Ideal for single cell
measurements
• Metal alloy fiber
• Tip Volume ~ 500µl
• Stainless steel/aluminum body
• Very sharp metallic tip for
insertion/penetration of tissue,
no extra needles or catheters
are needed, sensor is the needle
amiNO-30 Sensor
• 30 µm tip diameter
• For space restricted
measurements
• Metal alloy fiber
• Tip Volume ~ 10µl
• Longest life NO electrode
(user replaceable membranes)
• Tough enough for training
new staff and students
• Used to measure high levels
of NO as in drug solutions
and macrophages
• Tip Volume ~ 250µl
• Sub nano-molar detection limit
• Special membrane survives
numerous insertions
• Very stable, low background
current
• Tip Volume ~ 6µl
amiNO-100 Sensor
•
•
•
•
amiNO-600 Sensor
• 600 µm tip diameter
amiNO-FLAT Sensor
• Designed specially for
measuring NO released from
monolayer cultured cells
and large flat tissues
• Exceptional sensitivity and
baseline stability
• Rugged membrane
• Very rugged membrane,
flexible body
• Low background
• Highest sensitivity
BS4 69-0016 ‘SensoReady’ Electrode Polarizer
Please refer to the guide below to help you
select the best sensor for your application.
For additional assistance, please call
Harvard Apparatus Technical Support.*
Cell Biology
100 µm tip diameter
Extremely stable background
Resists breakage
To be used in restricted areas
and ‘in vivo’ measurements
• Very consistent & reproducible
• Tip Volume ~ 100µl
• Higher sensitivity, up to 100
times more sensitive than other
commercially available sensors
*The selection guide below is based on design, sensitivity and economy. For example, amiNO-FLAT and
amiNO-IV are the most sensitive but are also the
most expensive. The amiNO-600 is less sensitive but
is more economical.
• Very durable
• Tip Volume ~ 4µl
amiNO Sensor Selection Guide*
Application
First Choice(s)
Second Choice(s)
Measuring NO released from cells/large flat tissue
amiNO-FLAT
amiNO-600 or amiNO-IV
In vivo measurements and other insertion applications
amiNO-IV
amiNO-600
Measuring NO in small volumes or restricted space
amiNO-100
amiNO-7, amiNO-30 or amiNO-600
Measuring micro-molar levels of NO
amiNO-2000
amiNO-100 or amiNO-30
Following the decomposition of an NO producing compound
amiNO-600 or amiNO-100
amiNO-2000 or amiNO-IV
Measuring very low levels of NO (below 50 nM)
amiNO-600 or amiNO-IV
amiNO-100
Indirect measurement of Nitrate and Nitrite
amiNO-600, depending on
expected level
amiNO-100 or amiNO-2000
Training new staff or students
amiNO-2000
amiNO-600
* Note: Room Temperature Sensitivities — The above selection guide is based on design, sensitivity and economy. For example, amiNO-FLAT and amiNO-IV are the most sensitive, but
are also the most expensive.The amiNO-600 is less sensitive, but is more economical.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M3
Nitric Oxide
NEW
inNO-T Nitric Oxide Measurement System
Specifications
Catalog No.
$
Product
Current Scale
±2,500,000.0 pA
BS4 69-0013
24.00
Electrolyte
Concentration Scale
±1,000,000.0 nM
BS4 69-0014
240.00
Resolution
0.01 nM (0.1 pA)
Replacement Membranes for BS4 69-0012,
pkg. of 4
Screen Meter
7.5 digit meter
BS4 69-0016
121.00
SensoReady Electrode Polarizer
Recording Speed
1 to 1000 sec/div
BS4 69-0029
109.00
Power
Replaceable battery
Adapter, LIMO Connector to amiNO Sensor
Connector
Dimensions, H x W x D
76 x 229 x 203 mm (3 x 9 x 8 in)
Temperature Range
0° to 50°C, inNO-T only
Note: inNO and inNO-T are battery operated.Voltage selection is for Magnetic Stirrer.
Call Harvard Apparatus Customer Service for Nitrate Reduction Products.
Temperature Resolution 0.01°C, inNO-T only
Temperature Sensor
< 0.50 mm tip, 120 mm long, inNO-T only
Specialized Tools For Bioresearch
amiNO Sensor Performance Comparison and Ordering Information
Catalog No.
$
BS4 69-0008
301.00
BS4 69-0009
BS4 69-0010
388.00
BS4 69-0011
BS4 69-0012
437.00
BS4 69-0006
BS4 69-0007
601.00
Quantity
Model
Size
Typical Sensitivity
Competitor Typical Sensitivity
2
amiNO-7
7 µm
0.5-3 pA/nM (typical 1.5 pA/nM)
n/a
2
amiNO-30
30 µm
2-6 pA/nM (typical 4 pA/nM)
n/a
2
amiNO-100
100 µm
25 to 50 pA/nM (typical 35 pA/nM)
No Equivalent
2
amiNO-600
600 µm
150 to 250 pA/nM (typical 200 pA/nM)
No Equivalent
1
amiNO-2000
2 mm
Typical 1 pA/nM
Typical 1 pA/nM
2
amiNO-IV
600 µm
150 to 250 pA/nM (typical 200 pA/nM)
No Equivalent
1
amiNO-FLAT
See diagram
250 to 700 pA/nM (typical 400 pA/nM)
No Equivalent
inNO-T Nitric Oxide Measurement Systems
Sensors and Accesories
Catalog No. $
System Components
Premium System #1
115 VAC, 230 VAC,
60 Hz
50 Hz
BS4 69-0012 437.00
amiNO-2000 Nitric Oxide Sensor,
2 mm Tip
•
•
BS4 69-0010
amiNO-100 Nitric Oxide Sensor,
100 µm Tip, pkg. of 2
•
•
BS4 69-0011 494.00
amiNO-600 Nitric Oxide Sensor,
600 µm Tip, pkg. of 2
BS4 69-0006
amiNO-IV Nitric Oxide Sensor, InVivo 600 Micrometer Tip, pkg. of 2
BS4 69-0007 601.00
amiNo-FLAT Nitric Oxide Sensor,
Flat 25 x 25 mm, pkg. of 1
BS4 69-0013
Electrolyte for BS4 69-0012
BS4 69-0014 240.00
Replacement Membranes for
BS4 69-0012, pkg. of 4
BS4 69-0037
Nitrite Standard, 50 ml
BS4 69-0038
17.00
BS4 69-0016
BS4 72-0460
SensoReady Electrode Polarizer
21.00
BS4 72-1920
BS4 72-1921
BS4 72-1922
Calibration Vials, pkg. of 5
Spin Bar Magnetic Stir Bar,
5 x 2 mm, pkg. of 5
Magnetic Stirrer, 115 VAC
75.00
•
•
•
•
Premium System #3
115 VAC, 230 VAC,
60 Hz
50 Hz
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Premium System #4
115 VAC, 230 VAC,
60 Hz
50 Hz
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Magnetic Stirrer, 230 VAC
Electrode Holder and Stand
Premium System #2
115 VAC, 230 VAC,
60 Hz
50 Hz
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Systems
M4
1-Ch inNO System with inNO-T
Meter with Temp. Compensation
BS4 69-3050 BS4 69-3051 BS4 69-3052 BS4 69-3053 BS4 69-0048 BS4 69-0049 BS4 69-0050 BS4 69-0051
$ 7,134.00
7,134.00
7,134.00
7,134.00
7,134.00
7,134.00
7,134.00
7,134.00
2-Ch inNO System with inNO-T
Meter with Temp. Compensation
BS4 69-3054 BS4 69-3055 BS4 69-3056 BS4 69-3057 BS4 69-3058 BS4 69-3059 BS4 69-3060 BS4 69-3061
$
11,000 Specialty Products to Enhance Your Bioresearch
Nitric Oxide
NEW
Nitrate Reductor
•
Never needs reactivation, even
after hundreds of samples!
•
No need to bring the sample to the
reductor; take the reductor to the
sample. This eliminates the need
for sample transfer which saves
time and eliminates sample loss
•
Can be used in any sample container such as a vial, tube, micro-plate
well or spectrophotometric cell
•
Very small; virtually no minimum
sample volume restriction
required... It can be used in 50
microliters or 5 milliliters
•
100% conversion in minutes
•
Very economical
•
Reusable
Our nitrate reducing wire is based on a
multi-metal alloy structure. This multimetallic structure preserves the activity of
the reductor and enhances the rate of electron transfer in these chemical reactions:
M → M+2 + 2 e
NO - + 2 e + H O → NO - + 2 OH 3
2
2
The measurements of nitrate and nitrite
anions in biological samples have gained
more interest due to their association with
the nitric oxide (NO) pathway. Since these
ions are the oxidation products of NO, their
presence can be used as an indicator of NO
production activities.
There are many methods available for both
direct and indirect measurement of nitrite.
Unfortunately, this is not the case for nitrate
due to its different chemical reactivity.
Therefore, most of the analytical procedures
involving nitrate include its conversion to
nitrite using either enzymatic reduction or
cadmium-based reductors. These conversion procedures are tedious, time consuming, expensive, require multiple sample
transfer, and suffer from diminished activity.
Our NEW Nitrate Reductor is specifically
designed to overcome these shortcomings.
Principle of Operation
Conventional bimetallic composition based
reductors are based on copper-plated cadmium beads. The presence of copper metal
alters the potential energy of the cadmium
electrons enhancing the rate of their transfer
to nitrate. Even though this design has been
used successfully for many years, it has two
main drawbacks. The loss of its catalytic
activity after using it on a number of samples
requires that the reductor be reactivated. The
second problem deals with the difficulty in
handling and utilizing the beads for use with
very small sample volume containers to
avoid diluting the sample because these
beads are normally packed in a column.
The reductor is available in two different kit
forms. The manual kit includes 6 reductors that
are used to reduce nitrate in 6 samples simultaneously. The motorized kit is a combination
of a reductor and a mixer. The mixer is the
reductor! The reductor is the mixer. It is ideal
for use in sample containers where stirring is
difficult. The motorized reductor reduces
nitrate faster than the manual kit version. The
motorized version has a removable reductor
and comes with a micro-sleeve enabling the
user to utilize it as a micro-mixer in the lab.
Catalog No.
$
Cell Biology
This alloy structure completely eliminates the
need to ever reactivate the nitrate reductor. The
design of the reductor in the form of a wire
enables it to be used on very small sample volumes and removes the restriction of having to
move your sample to a specialized container.
Product
BS4 69-0054
271.00 Manual Nitrate
Reductor Kit, pkg. of 6
BS4 69-0055
217.00 Motorized Nitrate
Reductor Kit
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M5
Dissolved O2
NEW
Dissolved Oxygen Meter and Electrodes
BS4 69-0026 Dissolved Oxygen Meter shown with
Stirrer and Electrode Stand, not included
2 mm Oxygen Electrode
Specifications
Cable and Connector 3.5 mm x 6 ft (D x L)
Handle
6.4 x 50 mm (D x L)
Tip
Stainless steel, 2 x 70 mm
(D x L) with flat sensing tip
Specialized Tools For Bioresearch
Cat No.
Product
322.00
Oxygen Electrode, 2 mm
BS4 69-0028
218.00
Replacement O2
Membranes, pkg. of 4
Dissolved Oxygen Meter
This dissolved oxygen measuring system utilized the latest technologies in electronic circuitry and chemical sensors. Using the 2 mm
• Measures O2 in either liquids or
stainless steel-tip electrode oxygen can be
gas phase
measured in either liquid solution or gas phase.
• Battery operated/isolated meter
The BS4 69-0027 2 mm stainless steel electrode, see to the right, is supplied with each sys• Replaceable membranes
tem with two replacement membranes and
electrolyte. The meter can display current, percentage, or PPM oxygen. The meter offers an activation function to restore the activity of the sensor without the need of polishing and conditioning.
•
Meter offers a “clean” function to
reactivate the electrode
For more Oxygen Detection
Systems, see pages M7–M14.
Sub-millimeter sensors are
available by special order.
Specifications
% O2
0 to 100%
PPM
0 to 20
Current Range
0 to 2000 nA
Resolution
0.1 PPM, 0.1 %
Accuracy
±1%
Recorder Output
Analog output 0 to 5 V
Electrode Response Time
Under 10 sec, 90% response in stirred solution
Display
3.5 digit LCD
Power
120/240 VAC
Dimensions
20.3 x 22.9 x 76.2 cm (8 x 9 x 3 in)
Shipping Weight
2 lb (1.5 kg)
Catalog No.
BS4 69-0026
BS4 69-0028
M6
$
BS4 69-0027
$
Product
1,262.00 Oxygen Meter with Temperature Compensation and 2 mm Oxygen Electrode
218.00 Replacement O2 Membranes, pkg. of 4
BS4 72-1920
75.00 Magnetic Stirrer, 115 VAC
BS4 72-1921
75.00 Magnetic Stirrer, 230 VAC
BS4 72-1922
75.00 Electrode Holder and Stand
11,000 Specialty Products to Enhance Your Bioresearch
Dissolved O2
Phosphorescence O2 Measurement System
BS4 69-0502
• Two built-in light sources; one for surface (0.5 to 1 mm) and
one for deep tissue (2 to 3 cm) measurements
• Noninvasive oxygen measurements in tissue in-vivo or in-vitro
• Linear relationship between 1/lifetime and pO2
• Stable over time, no sensitivity or baseline drift
• Absolute calibration; calibrate only once
• Windows operating environment
• Temporal resolution < 1.2 sec/measurement
• Evaluate a wide range of tissue physiology and pathology
— Vascular and cardiopulmonary function
— Tumor oxygenation
— Status of skin grafts
— Diabetic vascular complications
— Ischemia/reperfusion injury, etc.
PMOD 2000
The system has two channels (sources) of excitation light. The standard system comes with one wavelength set to 524 nm (suitable for the
porphyrin based phosphors) and 635 nm (suitable for the tetrabenzoporphyrin based phosphors). Other combinations of excitation wavelengths are available. Please call our technical sales department for further information.
Phosphorescence emission is in the range of 660 to 760 nm (porphyrins) or 760 to 900 nm (tetrabenzoporphyrins). Since bright ambient light can saturate the detector, it is recommended that the work
area be lighted with DC powered monochromatic light sources.
The ‘Cool Colors’ LED lamps are specifically designed for this purpose. When an appropriate lamp is used, this light does not interfere
with the phosphorescence measurements.
• Measurements in-vitro in sealed glass chambers, no oxygen
exchange with the environment
Types of Phosphors and Phosphor Kits Available
Pd-meso-tetra (4-carboxyphenyl) porphyrin
This phosphor (Oxyphor RO) is dissolved in the presence of 0.5% or
greater of bovine serum albumin. It provides excellent oxygen sensitivity and has been accurately calibrated (see Lo et al., 1996). This
phosphor has an absorption band near 524 nm and emission near
690 nm and can be used for surface O2 measurements in tissues.
Lyophilized Porphyrin Kit*
1.2 g. approx. amount needed for approx. 30 rat (rats weighing
300 g each) and 10 cat (cats weighing 3 kg each) experiments using
the PMOD instrument. Lyophilized porphyrin contains (approx.):
9% Pd-meso-tetra (4-carboxyphenyl) porphine which is bound to
80% BSA (clinical grade, low fatty acid), 8% NaCl, and 3% TRISMA
buffer. Accessories include one (1) 10 cc syringe, three (3) syringe
filters (25 x .22 micron), and three (3) 4.0 mL amber glass vials with
foil caps.
Cell Biology
The PMOD-2000 uses a noninvasive optical method for detecting the
concentration of O2 in tissue. The operating principle for the unit is
based on the quenching of phosphorescence by oxygen in phosphors that have been injected into the blood. To determine the concentration of O2 in the area of tissue being measured, phosphorescence
lifetime measurements are taken using a sinusoidally modulated light
source for excitation and frequency lock for phosphorescence detection. The PMOD operates over a frequency range from 200 to 20,000
Hz, allowing measurements of phosphorescence lifetimes from <15
microseconds to 1.5 milliseconds. Measurements can be carried out
either at constant frequency or constant phase shift. This technique
differs from other methods of O2 detection in that this method is totally noninvasive, with the fiber optic bundle outside of the tissue in
which the O2 is being measured. The PMOD-2000 has a very high
accuracy throughout the physiological range and provides real time
measurements with high temporal resolution (~1 sec/measurement). The phosphors that are used with this system have absolute
calibration and follow Stern-Volmer behavior. Once a phosphor is
calibrated for a given temperature and solvent conditions these values are stable, eliminating the possibility of measurement drift and
the requirement for re-calibration at the time of the measurement.
• Visible and near infrared phosphors for different tissue depth
measurements
Pd-meso-tetra (4-carboxyphenyl) porphyrin dendrimer
Oxyphor R2: This new water soluble phosphor has significant advantages over the parent compound for experiments in vivo. The quenching properties are similar to those of the parent compound with
respect to the effect of albumin. However, because it is highly water
soluble it can be injected into the blood as a simple solution in physiological saline. The pH dependence of Oxyphor R2 is also very small
over the physiological range (see Lo et al., 1997). Oxyphor R2 is
largely excreted in the urine in mice and other animals. In mice,
excretion occurs with a half time of less than 2 hours.
Pd-tetra-(4-carboxyphenyl) tetrabenzoporphyrin dendrimer
Oxyphor G2: This is a water soluble phosphor that has an absorption
band near 636 nm and emission near 800 nm and can be used for O2
measurements in tissue up to 3 cm thick.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M7
Dissolved O2
Phosphorescence O2 Measurement System
Specialized Tools For Bioresearch
The ‘Cool Colors’ LED lamps are specifically designed for this purpose. When an appropriate lamp is used, this light does not interfere
with the phosphorescence measurements.
The Windows compatible software included with the PMOD-2000 system is used to configure the system’s parameters and to display and
store the measurement frequency, signal amplitude, phase, and time
of measurement as well as the oxygen pressure. The time course of
phosphorescence lifetime or oxygen pressure can be continuously
plotted on screen as the data are taken. All of the displayed signal
parameters can be saved to named files for further analysis etc. The
files are in ASCII and are easily read by other programs such as
Excel®, Sigma Plot®, etc. Systems come complete with a 6 mm OD
bifurcated optical fiber cable. Other optical fiber configurations are
also available, please call our technical sales department for additional information.
Specifications
Detection Range
Air Saturation to < 0.1 Torr
Sensitivity
1 part in 103
Temporal Resolution
Minimum 20 msec with measurements repeatable
each 1.2 sec
Temperature Dependence
Approximately 3% per degree Celsius
Power
Wall Transformer 100/230 VAC, 50/60 Hz
Dimensions
26 x 33.7 x 14 cm (10.25 x 13.25 x 5.5 in)
Weight
5 kg (11 lbs)
Catalog No.
BS4 69-0502
$
Product
10,910.00 PMOD-2000 Phosphorescence Oxygen
Measurement System
BS4 69-0503
70.00 Cool Color Lamp, Amber (592 nm maximal intensity),
115 VAC, 60 Hz
Computer Requirements: The operating software is for DOS or
Windows 95/98/NT and requires an IBM compatible PC. Only the
Windows NT version will allow determination of the distribution of
phosphorescence lifetimes. In any case, communication with the
computer requires a 16550 UART serial port (or later).
BS4 69-0504
70.00 Cool Color Lamp, Amber (592 nm maximal intensity),
230 VAC, 50 Hz
BS4 69-0505
70.00 Cool Color Lamp, Red (638 nm maximal intensity),
115 VAC, 60 Hz
BS4 69-0506
70.00 Cool Color Lamp, Red (638 nm maximal intensity),
230 VAC, 50 Hz
Method Reference Publications:
BS4 69-0507
85.00 Cool Color Lamp, Green (524 nm maximal intensity),
115 VAC, 60 Hz
BS4 69-0508
85.00 Cool Color Lamp, Green (524 nm maximal intensity),
230 VAC, 50 Hz
1) Vanderkooi, J.M., Maniara, G., Green, T.J., and Wilson, D.F. An optical method for measurement of dioxygen concentration based on quenching of phosphorescence. J. Biol.
Chem. 262: 5476-5482, 1987.
2) Wilson, D.F., Rumsey, W.L., Green, T.J., and Vanderkooi, J.M. (1988) The oxygen dependence of mitochondrial oxidative phosphorylation measured by a new optical method
for measuring oxygen. J. Biol. Chem. 263: 2712-2718.
3) Robiolio, M., Rumsey, W.R. and Wilson, D.F. (1989) Oxygen diffusion and mitochondrial respiration in neuroblastoma cells. Amer. J. Physiol., 256: C1207-C1213.
4) Shonat, R.D., Wilson, D.F., Riva, C.E., and Pawlowski, M. (1992) Oxygen distribution in
the retinal and choroidal vessels of the cat as measured by a new phosphorescence
imaging method. Applied Optics 31, 3711-3718.
5) Vinogradov, S.A. and Wilson, D.F. (1995) Metallotetrabenzoporphyrins. New phosphorescent probes for oxygen measurements. J. Chem. Soc. Perkin Trans. II, 2, 103-111.
6) Vinogradov, S.A., Lo, L.-W., Jenkins, W.T., Evans, S.M., Koch, C., and Wilson, D.F. (1996)
Non invasive imaging of the distribution of oxygen in tissue in vivo using near
infrared phosphors. Biophys. J. 70, 1609-1617.
7) Lo, L.-W., Koch, C.J., and Wilson, D.F., (1996) Calibration of oxygen dependent quenching of the phosphorescence of Pd-meso-tetra (4-carboxyphenyl) porphine: a phosphor
with general application for measuring oxygen concentration in biological systems.
Analy. Biochem. 236, 153-160.
8) Lo, L.-W., Vinogradov, S.A., Koch, C.J., and Wilson, D.F. A new, water soluble, phosphor for
oxygen measurements in vivo. Adv. Exptl. Med. Biol. 428, 651-656, 1997.
9) Vinogradov, S.A., Lo, L.-W., and Wilson, D.F., (1999) Dendritic polyglutamic porphyrins:
probing porphyrin protection by oxygen dependent quenching of phosphorescence.
Chem. Europ. J., 5 (4), 1388-1347.
BS4 69-0509
322.00 Phosphor, Pd-meso-tetra (4-carboxyphenyl)
porphyrin, 1 gm Vial (Oxyphor RO)
BS4 69-0510
791.00 Phosphor, Pd-meso-tetra (4-carboxyphenyl) porphyrin
dendrimer, 1 gm Vial (Oxyphor R2)
BS4 69-0511
315.00 Phosphor, Pd-tetra (4-carboxyphenyl)
tetrabenzoporphyrin dendrimer (Oxyphor G2), 20 mg
BS4 72-6180
355.00 Phosphor, Pd-tetra (4-carboxyphenyl)
tetrabenzoporphyrin dendrimer (Oxyphor G2), 50 mg
BS4 72-6181
599.00 Phosphor, Pd-tetra (4-carboxyphenyl)
tetrabenzoporphyrin dendrimer (Oxyphor G2), 100 mg
Selected Application Reports: (selected from more than 60)
1) Yonetani, M., Huang, Ch-Ch., Lajevardi, N., Pastuszko, A., Delivoria-Papadopoulos,
M.,and Wilson, D.F. (1994) Effect of hemorrhagic hypotension on extracellular level of
dopamine, cortical oxygen pressure and blood flow in brain of newborn piglets.
Neuroscience Letters, 180, 247-252.
2) Rumsey, W.L., Pawlowski, M., Lejavardi, N., and Wilson, D.F. (1994) Oxygen pressure
distribution in the heart in vivo and evaluation of the ischemic “border zone”. Am. J.
Physiol. 266: H1676-1680.
3) Huang, Ch-Ch., Yonetani, M., Lajevardi, N., Delivoria-Papadopoulos, M., Pastuszko, A.
and Wilson D.F. (1995) Comparison of post-asphyxial resuscitation with 100% and
21% oxygen on striatal dopamine metabolism in newborn piglets. J. Neurochemistry,
64, 292-298.
4) Song, D., Olano, M., Wilson, D.F., Pastuszko, A., Tammela, O., Nho, K., and Shorr, R.G.L.
(1995) Comparison of the efficacy of blood and polyethylene glycol-hemoglobin in
recovery of newborn piglets from hemorrhagic hypotension: effect on blood pressure,
cortical oxygen, and extracellular dopamine in the brain. Transfusion 35, 552-558.
5) Poole, D.C., Wagner, P.D., and Wilson, D.F. (1995) Diaphragm microvascular plasma PO2
measured in vivo. J.Appl. Physiol. 79(6), 2050-2057.
6) Olano, M., Song, D., Murphy, S., Wilson, D.F., and Pastuszko, A. (1995) Relationships of
dopamine, cortical oxygen pressure, and hydroxyl radicals in brain of newborn piglets
during hypoxia and posthypoxic recovery. J. Neurochem. 65, 1205-1212.
7) Cerniglia, G.J., Wilson, D.F., Pawlowski, M., Vinogradov, S.A., and Biaglow, J. (1997)
Intravascular oxygen distribution in subcutaneous 9L tumors and radiation sensitivity. J.Appl. Physiol. 82(6), 1939-1945.
8) Rumsey, W.R., Abbott, B., Lo, L.-W., Vinogradov, S.A., and Wilson, D.F. (1997) Imaging of
oxygen in the surface and deep areas of the kidney. Adv. Exptl. Med. Biol. 411: 591-596.
M8
11,000 Specialty Products to Enhance Your Bioresearch
Dissolved O2
FO2ton
BS4 69-0450 FO2ton Fiber Optic
Oxygen Sensing System
The thin film ruthenium technology used in
the probe tips for detecting oxygen does not
consume oxygen, allowing the sensor tip to
be in continuous contact with the sample.
FO2ton Oxygen Sensors work in various sample types including viscous samples. Since
this technology is immune to interference
caused by pH or salinity change or from
changes in ionic strength, they can be used in
a wide range of experiments. The sensors are
designed to have a high resistance to bio fouling. Temperature compensation via an external temperature probe provides added accuracy and stability to the measurement of oxygen is samples were temperature may vary.
The FO2ton Fiber Optic Oxygen Sensing
System uses the fluorescence of an organic
ruthenium based dye to measure the absolute
concentration of oxygen: Optical fiber carries
excitation light produced by an integrated
blue LED light source to the thin-film ruthenium dye coating at the fiber optic probe tip.
Fluorescence generated at the tip is collected
by the probe and carried back by the fiber to
a high sensitivity spectrometer. When oxygen
in the gas or liquid sample diffuses into the
thin-film ruthenium coating, the fluorescence
is quenched. The degree of quenching correlates to the concentration of oxygen.
Ruthenium tipped sensors are available in different sizes and styles of fiber optic sensors to
meet a number of application.
The FO2ton Fiber Optic Oxygen Sensing
System consists of a high-sensitivity miniature
fiber optic spectrophotometer, light source,
USB interface to connect the system to an IBM
compatible benchtop or portable computer
and a selection of various size probes. With
this combination, a full spectral analysis of
dissolved and gaseous oxygen concentration
can be achieved.
The high-sensitivity fiber optic spectrometer
can be used in a stand-alone mode to measure fluorescence, absorbance, reflectance
and radiance, and other types of standard
spectrometric measurements through the use
of optional fiber optic accessories. Please call
our Technical Sales Department for details.
Oxygen sensing based on Ruthenium
fluorescence detection and fiber
optic technology
•
Does not consume oxygen,
allowing for continuous contact
with the sample
•
Wide variety of fiber optic sensors
sizes available
•
Compact system combines a
high-sensitivity spectrophotometer,
computer interface (USB) and
FO2ton Windows™ 98/NT
compatible software
•
Small size and 12 VDC power makes
it ideal for portable and field use
•
Frequently asked questions sheet
available
Is the probe influenced by
ambient light?
The probe will be affected by ambient light.
All probe types should be shielded from
ambient light by installation in a closed vessel, or by shielding the probe with a dark
housing. Our FOXY-T1000 probe has a
screw-on cap to block the optical field of
view of the fiber, while allowing easy access
to the probe tip.
An overcoat of silicone can be applied to the
probe (at no charge) to help reduce ambient
light effects. The overcoat slows the response
of the probe to 30-50 seconds in liquids and
20-30 seconds in gases.
Cell Biology
The FO2ton oxygen sensing system detects
oxygen (pO2) in both solutions or in air
through the use of a ruthenium fluorescence
and Fiber Optic Technology. The FO2ton system offers high sensitivity, reversability and
stability in the detection of oxygen in numerous applications. Its low power requirements and small package size makes it ideal
for remote monitoring, field applications,
and other applications where size and/or
portability are required.
•
Harvard Apparatus
now owns the
Navicyte line of
Ussing and Diffusion
Chambers. See pages
M166 to M170 for
complete details on
those products and
many others.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M9
Dissolved O2
FO2ton Fiber Optic Oxygen Sensing System
Specialized Tools For Bioresearch
Specifications
State
Dissolved Oxygen
Gaseous Oxygen
S/W Display
(%, mmHg, ppm)
(%, mmHg, ppm)
Minimum Detectable Limits
0.44 mmHg (0.023 ppm)
0.44 mmHg (0.06%)
Dynamic Range
0 to saturation level*
0 to 100%
Resolution
0.11 to 0.37 mmHg for dissolved oxygen partial pressure
0 to 160 mmHg gas
0.015 to 0.045 % (0.11 to 0.37 mmHg) for oxygen partial pressure
0 to 160 mmHg
Interference
No interference from pH change or salinity
No interference from CH4, acetone, moisture or CO2
Resolution:
High O2 Concentration
Low O2 Concentration
0.02 ppm
0.003 ppm
0.4%
0.01%
Stability Drift
< 0.02 ppm per day
< 0.05% per day
Response Time
< 1 sec (< 30 seconds with silicone overcoat)
< 1 sec (< 30 sec with silicone overcoat)
Compensation
For temperature only
For temperature only
Calibration
2 point calibration. First point in oxygen free gas or liquid, second point in air or air saturated water, or liquid
Effect of Error in
1% error in reading of 0 concentration standard = 1.6 mmHg error in low DO concentration, 0 mmHg error in high end
Calibration on Answers
1% error in reading of high concentration standard = 4 mmHg error in high concentration, 0 mmHg error in low end
Replaceable Probe
Yes
Yes
Probe Reconditioning
Yes
Yes
Re-Calibration
Only when probe is replaced
Only when probe is replaced
Probe Temperature Range
-80° to 80°C (110˚C briefly)
-80° to 80°C (110˚C briefly)
Storage Conditions
No specific requirements
No specific requirements
Probe Lifetime
~1 year
~1 year
Chemical Compatibility
1) Unaffected by pH<10 or salinity changes
2) Safe with 50% methanol, hexane, CH4, sodium sulfite, acids
3) Not recommended with strong bases (pH>10), ethanol, acetone, acetonitrile and HF and BTEX solvents
* 160 mmHg or 8.4 ppm for water saturated with air, 760 mmHg or 41 ppm at 25°C for water saturated with oxygen, > 760 mmHg for water supersaturated with oxygen.
Catalog No.
BS4 69-0479
$
Product
5,524.00 FO2ton Fiber Optic Oxygen Sensing System, 115/230 VAC, 50/60 HZ
Replacement Parts
BS4 69-0452
702.00 Fiber Optic Oxygen Probe Stainless Steel Probe, 1000 µm core D
BS4 69-0458
572.00 Bifurcated Optical Fiber Assembly, 600 µm
BS4 69-0459
22.00 Splice Bushing
BS4 69-0463
BS4 69-0464
156.00 Temperature Sensor
2,974.00 Fiber Optic Spectrometer for Fluorescence
BS4 69-0465
702.00 Blue Light Source for USB F.O. SP
BS4 69-0476
441.00 OOI Sensors SW-O2 & PH Anal, 32 BIT
BS4 69-0477
382.00 Factory Calibration SVC for FOXY Sensors
Accessory Parts
BS4 69-0453
BS4 69-0454
BS4 69-0455
545.00 Fiber Optic Oxygen Probe Polyimide Probe, 600 µm core D
754.00 Fiber Optic Oxygen Probe, Aluminum Probe, 500 µm OD
1,481.00 Fiber Optic Oxygen Probe, Rugged 1 µm D Silica-Core Fiber in 0.25 in OD Stainless Steel Ferrule, with Screw-On Light Shield
BS4 69-0456
677.00 Bifurcated Optical Fiber Assembly, 200 µm
BS4 69-0457
545.00 Bifurcated Optical Fiber Assembly, 400 µm
Harvard Apparatus owns Hugo Sachs Elektronik. The HSE isolated organ baths set
the industry standard. They can be found in the Isolated Organ and Tissue Section K.
M10
11,000 Specialty Products to Enhance Your Bioresearch
Dissolved O2
Universal Oxygen and Temperature Monitor
•
Used to measure oxygen partial pressure (pO2) in
gases, fluids, suspensions and biological media
•
Determination of saturation or pressure of
dissolved oxygen in fluid
•
Small, battery operated and water resistant
• Ideal for:
— Cell culture media for controlling microbial
growth conditions in science and
pharmaceutical industry
— Living tissue and body fluids for biomedical
research and veterinary medicine
— Plants, fruits and meat for food science and
technology
— Mud, silt and soil for toxicological and
environmental monitoring
The LICOX MCB® is made for oxygen measurements with the
flexible REVOXODE catheter micro probes. The temperature
is compensated by thermocouple microprobes that have a
built-in flexible polyethylene or metal tube for more than
one year of use. Every probe is delivered sterile and can be
easily calibrated within its sterile protection cover.
Tip of REVOXODE p02 Probe C1
5 mm
BS4 69-0188 Calibration Device for REVOXODE
The oxygen display of LICOX MCB® is calibrated by amplifier gain adjustment while the probes are exposed to the
reference atmosphere or fluid. Thus the display can be
adjusted to absolute pO2 values in mmHg or hPa (if the pO2
of the calibration chamber is calculated from a formula
given in the instruction manual) or to relative values where
the oxygenation in the calibration chamber is set as 100%.
The LICOX MCB® system includes BS4 69-0189 and BS4
69-0190 probe cables, BS4 69-0197 and BS4 69-0198 output cables, BS4 69-0185, BS4 69-0186 or BS4 69-0187
power supply, BS4 69-0183 rechargeable batteries and
BS4 69-0201 probe.
Cell Biology
For measurements with LICOX MCB® a REVOXODE oxygen
probe and a type-K thermocouple are simultaneously used.
Temperature and oxygen are displayed on LCD, illuminated
in the dark; the analog outputs can be used for recording the
data with the data collection system LICOX DAQ® or a paper
chart recorder. The REVOXODE probe is a new electrochemically reversible type of a polarographic microprobe. It
has a built in flexible polyethylene tube allowing for over 120
hours of continuous use. The probe is remarkably accurate
in long term use, in particular, at low oxygen levels. In the
oxygen circuit of LICOX MCB® the temperature is specifically compensated for REVOXODE probes.
Rear Panel of LICOX MCB ®
Probes are usually calibrated within humid chamber in protection cover which is delivered
with sterile probe. Cover is inserted into BS4 69-0188 calibration device. This is not required
for BS4 69-0201 REVOXODE because temperature probe can be directly inserted (sterile) into
oxygen probe.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M11
Dissolved O2
Universal Oxygen and Temperature Monitor
Specifications
Product
Classification
Lab equipment, , electrical safety meets IEC 61010-1,
EMI: 61000-4 part 2 to 6, EN 50204, EME: EN 55011
BS4 69-0180
4,646.00
LICOX MCB® Oxygen Monitoring System, 115 VAC,
USA
Principle
Polarographic oxygen probe circuit with temperature
compensation and type-K thermocouple amplifier
BS4 69-0181
4,646.00
LICOX MCB® Oxygen Monitoring System, 230 VAC,
Europe
Probe Input
0 to 10,000 nA (oxygen), 1 to 8 mV (temperature), both
circuits isolation amplifiers, breakdown voltage > 1.5 kV
BS4 69-0182
4,646.00
LICOX MCB® Oxygen Monitoring System, 230 VAC,
UK
BS4 69-0183
154.00
Rechargeable NiMH Batteries, pkg. of 4
BS4 69-0184
439.00
Tough Protection Pouch for Outdoor Measurement;
Hand Made Real Leather Bag with Front Panel Flap,
Accessory Pocket, Cable Protection and Strap
BS4 69-0187
201.00
Power Supply, USA, 110 VAC, 40/60 Hz
BS4 69-0185
201.00
Power Supply, Europe, 240 VAC, 40/60 Hz
BS4 69-0186
201.00
Power Supply, UK, 240 VAC, 40/60 Hz
BS4 69-0188
728.00
Calibration Device for REVOXODE Oxygen Probes,
Double Metal Barrel with Temperature Sensor
(Not required if using BS4 69-0201 Probe)
BS4 69-0189
274.00
Oxygen Probe Cable, Blue 2.2 m (7.2 ft)
BS4 69-0190
274.00
Temperature Probe Cable, Green 2.2 m (7.2 ft)
BS4 69-0191
201.00
Extension Cable for O2 Probe, Blue 1.5 m (4.9 ft)
BS4 69-0192
201.00
Extension Cable for Temp. Probe, Green 1.5 m (4.9 ft)
BS4 69-0195
93.00
BS4 69-0196
134.00
Analog Temp. Signal Output Cable with BNC Plug
BS4 69-0197
101.00
Analog O2 Signal Output Cable with 4 mm Plugs
BS4 69-0198
101.00
Analog Temp. Signal Output Cable with 4 mm Plugs
BS4 65-0445
148.00
pO2 Probe Offset Test Kit, 1 Glass Capillary with 10
Ampoules pO2 Zero Solution
Display:
Oxygen Range
1. 0 to 199.9 (mmHg, kPa or %)
2. 0 to 1999 (mmHg, hPa or %)
-199.9 to +199.9, useful range -40°C to 199°C
Temperature
Analog Signal Output:
Oxygen
Temperature
Calibration
Specialized Tools For Bioresearch
Catalog No. $
0 to 5 V, 5 mV/display unit
-0.4 to 2 V, 10 mV/°C
Adjustment of amplifier gain to sensitivity of REVOXODE
when placed in humid chamber of known temperature and
known (or reference) oxygen content; offset calibration not
required for REVOXODE; calibration of temperature
channel and probe by manufacturer
Accuracy and
Reproducibility:
Values determined if using probes BS4 69-0201 and
BS4 65-0426 in pO2 range of 0 to 150 mmHg at probe
temperatures between 15°C and 48°C and at instrument
temperatures between 15°C and 30°C
Oxygen Channel
Offset < 1 mmHg, offset drift < 1 mmHg / 120 hr
Probe sensitivity drift < 5% / 24 hr and < 10% / 120 hr
Temperature compensation error < 5%
Linearity error < 2 mmHg
Temperature Channel Absolute error < 0.5°C
Power Supply
9 V DC, max 700 mA during fast battery charging, EU and
UK: 240 VAC 50 Hz, USA: 110 VAC 50/60 Hz
Case Dimensions,
H xW x D
Water protected metal case IP64, 3 x 11 x 24 cm
(1.2 x 4.3 x 9.5 in)
Case Weight (Net
750 g (26.5 oz), net
Analog O2 Signal Output Cable with BNC Plug
LICOX MCB® Oxygen and Temperature Probes
Cat. No.
$
Length of
Distance from Tip
Sensitive Tip Area to Sensitive Area
Ø of Catheter
Near to Tip
Ø of Catheter
Near to Root
Probe
Length
Probe Description
C1
5 mm
7 mm
< 0.46 mm
< 0.6 mm
200 mm
Standard Use
C1.R
1 mm
2 mm
< 0.46 mm
< 0.6 mm
200 mm
Short Tip, High Resolution
C1.1
5 mm
7 mm
< 0.46 mm
< 0.6 mm
300 mm
Standard Use, Long
C1.G2
7 mm
5 mm
< 0.8 mm
0.8 mm
460 mm
Long and Very Robust
Model
REVOXODE Oxygen Probes
BS4 65-0415
BS4 65-0418
225.00
BS4 65-0416
BS4 69-0199
210.00
BS4 69-0200
BS4 69-0201
175.00
C1.SB
5 mm
5 mm
< 0.65 mm
0.95 mm
150 mm
PE Tube, 3 cm Steel Tube Shaft
C1.G3
4 mm
5 mm
< 0.8 mm
0.8 mm
330 mm
Y-Connector for Thermocouple
4 mm
< 0.8 mm
0.8 mm
126 mm
PE Tube, 3 cm Steel Shaft
Thermocouple Temperature Probe
BS4 69-0202
147.00
C8.B
3 mm
For further information on the use of LICOX for experimental biomedical research in brain, cerebrospinal fluid, muscle, connective
tissue, myocardium, tumors, gut, liver and other internal organs please contact Harvard Apparatus Customer Service.
M12
11,000 Specialty Products to Enhance Your Bioresearch
Dissolved O2
Harvard/Instech Biological Oxygen Monitoring Systems
BS4 61-0130 Dual Chamber Oxygen Monitoring System shown with
Stronghold™ stand and clamps (not included, see pages O22–O27)
Oxygen Electrodes
The BS4 61-0130 is a complete, flexible system designed for oxygen measurement in
small samples, as is frequently required in
biological research. The system is designed
around our miniature Clark-style polarographic electrode. Measuring only 1/8 of an
inch in diameter (3.2 mm), it is ideal for
metabolic studies of mitochondria, cell suspensions, and enzyme assays. This system
includes everything you need for oxygen
measurement on two independent channels:
a dual channel amplifier, two oxygen electrodes, and two chamber systems. It also
includes accessories to simplify electrode
maintenance. The chambers can be set up in
two basic configurations:
Our Clark style polarographic electrode
measures 41.3 mm (1.625 in) long by 3.2
mm (0.125 in) diameter. The cathode is an
exposed section of 0.1 mm (0.005 in) pure
platinum wire in a fused glass seal.
•
Sample sizes as small as 600 µl
•
Ideal for biological applications
•
Measures oxygen inline or in sealed
sample
Catalog No.
BS4 61-0130
Batch Cell Mode
For measuring oxygen consumption in a
sealed 600 µl sample. The batch cell chamber
includes a magnetically-coupled stirring system, connections for circulating water to
maintain a constant temperature, and a
unique window-valve for filling, rinsing, or
injecting solutions into the chamber. For continuous flow of water use our 66/77 Peristaltic
Pumps, see section A.
Component
4,848.00 Dual Chamber Oxygen
Measuring System
System Components
BS4 56-5077
1,450.00 Dual-Channel Amplifier,
qty. of 1
BS4 56-5002
425.00 Standard Oxygen
Electrodes, qty. of 2
BS4 56-4930
750.00 Batch/Flow Chamber
Systems, qty. of 2
BS4 61-0249
175.00 Magnetic Stirring Kits,
qty. of 2
BS4 61-0248
350.00 Dual-Channel Stirring
Controller, qty. of 1
BS4 61-0138
175.00 Chlorider and
Accessory Kit, qty. of 1
BS4 56-5028
45.00 Electrode Accessory
Kit, qty. of 1
Flow Cell Mode
For in-line measurement of oxygen in solutions flowing past the electrode. This set-up is
often used to measure oxygen uptake of perfused tissues by measuring inflow pO2 with
one channel, and comparing it with the outflow pO2 as measured by the second channel.
$
To prepare the electrode, a sheet of polyethylene membrane material is sealed with an O-ring
over a drop of KCl solution on the electrode tip.
The temperature coefficient is 4%/1°C, so temperature stability in your system is important.
Each electrode includes a cable which is
attached to the electrode and sealed to prevent salt contamination, a sleeve to use the
electrode in our chamber system, a sleeve to
use the electrode outside the chamber, extra
membrane material and o-rings, a membrane
installation tool, and polishing paper and
Scotch-Brite for maintaining the electrode.
This electrode is compatible with the
BS4 56-5077 amplifier and YSI’s model 5300
oxygen monitor (the cables are different, so
specify the appropriate part number). We
also supply a special Thurman-style electrode
for existing liver perfusion set-ups.
Catalog No.
Components of BS4 61-0130
Dual Chamber Oxygen Monitoring System
Cell Biology
Dual Chamber Oxygen Measuring System
$
Product
BS4 56-5002
425.00 Standard Electrode
with Cable
BS4 61-0134
450.00 Standard Electrode
with YSI Cable
(Replaces YSI Part
Numbers 5357 and
5358)
BS4 61-0136
540.00 Thurman Style
Electrode
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M13
Dissolved O2
Harvard/Instech Biological Oxygen Monitoring Systems
Electrode Chlorider
Our chlorider simplifies the process of electrically depositing a new layer
of Ag-AgCl on the pure silver tip of our electrodes. This layer can become
contaminated and will wear off under normal operating conditions. To
rechloride an electrode, simply fill the stainless steel cup with KCl solution and dip the electrode. The chlorider also includes extra electrode Orings, an O-ring installation tool, and various polishing papers.
Specialized Tools For Bioresearch
Catalog No.
Product
Batch/Flow Chamber System
BS4 61-0138
Each chamber system consists of an anodized aluminum chamber
block with connections for circulating water to keep the electrode and
sample at a constant temperature. The chamber can be set up for oxygen measurement in a fixed 600 µl sample by inserting the Kel-F batch
cell cup into the chamber block. The batch cell chamber has separate
ports for filling, overflow, and additions made with a micropipette or
syringe. A magnetic stirring assembly couples with a 2 x 7 mm stir bar
in the cup to keep fresh solution moving past the electrode tip.
Alternatively, the chamber can be set up for in-line oxygen measurement by inserting the appropriate flow cell into the chamber block.
Separate high and low flow cells cover a range of flow rates.
Oxygen System Replacement Parts
Catalog No.
$
Product
BS4 56-4930
750.00 Chamber Block with Batch and Flow Cells*
BS4 61-0137
350.00 Chamber Block with Flow Cells Only*
* Note: Electrode, stirring assembly and stirring controller not included. If needed, order
separately, or order part number BS4 61-0131 Instech Oxygen Electrodes.
175.00 Chlorider and Accessory Kit
BS4 61-0249
175.00 Miniature Magnetically Coupled Stirring Kit: Compatible
with BS4 56-4930 Batch Cell Chamber, controlled by
any Instech Stirring Controller; Includes 12 mm Motor
Assembly, Mounting Clip and Teflon Stirring Bar
BS4 56-5028
45.00 Accessory Kit for Electrode Maintenance: Includes 15
O-Rings, O-Ring Installation Tool, 24 in2 Polishing
Paper, 24 in2 Scotch Brite and 72 in2 Membrane
Material
BS4 56-5036
45.00 Electrode Cable, 107 cm
(42 in), for Instech Amplifiers
BS4 61-0139
60.00 Electrode Cable, 107 cm
(42 in), for YSI Amplifiers (Replaces YSI 5358)
BS4 61-0140
45.00 Output Cable, 107 cm (42 in), for Instech Amplifiers
BS4 61-0250
35.00 Teflon Stirring Bars, 2 x 7 mm, pkg. of 3
BS4 61-0248 Dual Channel Precision
Stirring Controller
Single Chamber Systems with Electrodes
For customers that already have an amplifier, we offer the following.
Catalog No.
$
Product
BS4 61-0131
1,650.00 Batch/Flow Chamber System for Instech Amplifiers
BS4 61-0132
1,675.00 Batch/Flow Chamber System for YSI Amplifiers:
BS4 61-0134 Standard Electrode with YSI
Compatible Cable
BS4 61-0133
1,675.00 Batch/Flow Chamber System for Diamond General
Chemical Microsensor: BS4 61-0135 Standard
Electrode with Diamond General Compatible Cable
Dual-Channel Oxygen Amplifier
This amplifier generates the potential that operates our standard electrodes BS4 56-5002 and also converts the nanoamp electrode current
into a voltage to measure pO2. It contains two independent channels, each
with gain and zero offset controls and analog outputs. A 3.5-digit LED display can be set with a selector switch to display either channel or the difference between the two. The amplifier measures, H x W x D, 10.8 x 26.7
x 22.2 cm (4.25 x 10.5 x 8.75 in) and weighs 1.6 kg (3.5 lbs). It operates on 120 V AC current, but can set for 220 V operation at the factory.
Catalog No.
BS4 56-5077
M14
$
$
Precision
Stirring
Controllers
Our BS4 61-0247 and
BS4 61-0248 Stirring
BS4 56-5077
Controllers are used to
Dual Channel Oxygen Amplifier
control the magnetic
stirring assembly in the
batch cell chamber. Each controller has an on/off switch, a power on
LED, and a dial to precisely regulate the motor speed from 200 to 5000
RPM. The controllers use a unique ‘pseudo tachometer’ feedback circuit which reduces electromagnetic interference. The BS4 61-0248 dual
channel is a lower cost per channel option than the BS4 61-0247. It is
also the most frequently ordered model since many oxygen experiments
require two channels.
Catalog No.
$
Product
BS4 61-0247
250.00 Single Channel Precision Stirring Controller
BS4 61-0248
350.00 Dual Channel Precision Stirring Controller
Product
1,450.00 Dual Channel Oxygen Amplifier
11,000 Specialty Products to Enhance Your Bioresearch
Respirometry
HSE-HA Oxystat System, Type 1000
Application
Stirrer
Motor
Stimulation Electrode
Connection
Port for Oxygen Electrode
with Shielding Electrode
The oxygen consumption VO2 of
small cell assemblies (e.g. papillary
muscle) or of isolated cells has usually in the past been calculated from
the reduction in the oxygen partial
pressure (pO2) in a ‘closed system’.
By contrast, the HSE-HA Oxystat
operates as an ‘open system with
feedback’. The pO2 (as the controlled variable) is maintained constant by the addition of solution with
known pO2. VO2 can be calculated
from the volume added and the pO2.
It is an important advantage of the
method that the pO2 is maintained
virtually constant during the experiment. In this way the oxygen consumption can be measured under
steady-state conditions at accurately
defined pO2 values, while at the same
time also measuring metabolic
parameters.
•
Unique open system
with feedback’ for
measuring oxygen
consumption of cells
(cardiomyocytes, hepatocytes, endothelial cells)
•
Measures down to
0.1 mmHg
•
Two operating modes
possible:
— Closed system oxygen
consumption calculated from pO2 reduction
— Open system
(measurement at
constant pO2)
controlled addition
of solution with
known pO2
•
Electrical stimulation
capabilities
•
Fitting for fiber optics for
easy Ca++ concentration or
redox potential
Port for
Fiber Optics
Water Jacket 37°C
Cell Biology
Connecting ports of the Oxystat chamber, for a working system it must be
equipped with a thermostat, the pO2 measurement electrode, the controller and the pump, optionally with fiber optics. The stimulation electrodes are built in.
Main Cell
Chamber
Chamber Cover with
Stirrer and Grid
Stimulation Electrode
Lower Inset with Grid
Stimulation Electrode
and Filter Holder
Main parts of Oxystat System. Lower inset can easily slide out; it receives filter paper and contains lower stimulation electrode. Upper cover is clipped on
chamber and contains stirrer with motor and upper stimulation electrode.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M15
Respirometry
HSE-HA Oxystat System, Type 1000
Oxystat
pO2
Measurement
Pump
Controller
Specialized Tools For Bioresearch
Compares Measurement
with Setpoint
Recorder
Continuous Recording of
pO2 VO2 and Fluorescence
Figure 1: Oxystat Chamber
Oxystat System Components
The Oxystat 1000 System includes the following components:
•
•
•
•
•
•
Oxystat Incubation Chamber with Stirrer BS4 73-0117
Power Supply for Stirrer BS4 73-0118 or BS4 73-0119
pO2 Electrode BS4 73-0120
Control Unit Model 719 with amplifier and Pump BS4 73-0122
Stimulator BS4 73-0124
Thermocirculator BS4 50-1932 or BS4 50-1940
All of these components must be purchased separately. In addition to
the above equipment, a chart recorder or data acquisition will permit
recording of data for further analysis. The Oxystat chamber is an incubation chamber with a volume of 10 ml (Figure 1). Mechanical damage to the cells during stirring of cell suspensions was an important
disadvantage of previous systems; special attention has therefore been
paid here to a very gentle stirring action. The cells are introduced into
the chamber and the chamber is filled with medium so as to be free
from air bubbles (the venting cannula is used to remove air bubbles
from the system). The pO2 electrode measures the fall in chamber pO2
due to the oxygen consumed by the cells. Since pO2 measurement is
seriously affected by temperature, the chamber is provided with optimal thermostating.
Operation of the Oxystat
The HSE-HA Oxystat can operate like conventional setups as a ‘closed
system’. The oxygen consumption is calculated from the slope of the
recorded pO2 reduction. Since the chamber is very well screened
against oxygen diffusion it is possible to measure pO2 down to 0.1
mmHg. The really new application of the Oxystat is however an ‘open
system’ with controlled addition of oxygen through the solution so that
O2 consumption can be measured at constant pO2.
M16
Figure 2: Oxystat System
The operation of the Oxystat as an open system is indicated in Fig. 2. The
pO2 signal is examined by the controller; if it differs from the preset
value a pump is operated to introduce oxygen-rich solution into the
chamber. This raises the pO2 in the chamber back to the set value and
the pump is then stopped again. As a result a flow equilibrium is established through which, despite the oxygen consumption of the incubated
cells, the chamber pO2 is maintained close to the set value (±5%). The
volume in the chamber is maintained constant since the additional volume flows away through an outlet filter. The volume introduced into the
Oxystat to maintain the set pO2 is used to calculate the oxygen consumption of the cells. A cannula permits withdrawal of samples for biochemical analysis without interfering with the measurement of oxygen consumption. As an option there is provision for fitting a fiber optic into the
chamber for simultaneous measurement of additional parameters such
as intracellular Ca++ concentration or redox potential. Two platinum
mesh fittings inside the chamber can be used for electrical stimulation
of muscle cells to experimentally increase the O2-consumption. A separate stimulator, see below, is required for this application.
Oxystat Measuring System Model 719
This system includes the Oxystat control unit and the pump. It consists of a titrator connected to the pO2 meter. The volume is displayed
and can be recorded.
Square Wave Stimulator G-270
It has been developed for the stimulation of isolated cardiomyocytes in
suspension. The main specifications are:
•
•
•
•
•
Output voltage 50 to 250 V
Maximum output current 7 A
Square wave biphasic stimulation pattern
Stimulation width 10 to 255 µsec
Stimulation frequency 0.1 to 20.0 Hz
11,000 Specialty Products to Enhance Your Bioresearch
Respirometry
HSE-HA Oxystat System, Type 1000
Figure 3: Original recording from incubated isolated cardiomyocytes in
Oxystat at available oxygen concentration of 90 mm Hg. Curve 1: added volume; Curve 2: chamber pO2. Arrows: Instants of sampling for biochemical
analysis.
Typical Recording
Oxystat System
For a complete Oxystat System, please order one each of the
following components:
BS4 73-0117
BS4 73-0118 or BS4 73-0119
BS4 73-0120
BS4 73-0122
BS4 73-0124
BS4 50-1932 or BS4 50-1940
Catalog No.
References:
1) Stumpe T. and Schrader J.: Phosphorylation potential, adenosine formation, and critical PO2 in stimulated rat cardiomyocytes, Am.J.Physiol. 273, H756-H766, 1997
Product
3,478.00 Oxystat Chamber, Thermostated, Volume 10 ml,
with Built-In Stirrer, Requires BS4 73-0118 or BS4
73-0119 Power Supply for Stirrer
BS4 73-0118
181.00 Power Supply for Stirrer with Adjustable Voltage,
115 VAC, 60 Hz
BS4 73-0119
54.00 Power Supply for Stirrer with Adjustable Voltage,
230 VAC, 50 Hz
Typical Application
The relationship between available oxygen and oxygen consumption of
isolated cardiomyocytes has been investigated under different forms of
stimulation (Fig. 4). Resting cardiomyocytes (triangles) have a constant
oxygen consumption over a wide pO2 range (1 to 120 mm Hg). It is only
below a so-called ‘critical pO2’, here at 1 mm Hg, that the oxygen consumption decreases with decreasing available oxygen. Electrical stimulation of the cells at a frequency of 9 Hz produces a 3-fold increase in
oxygen consumption (rhombus). Here again the oxygen consumption
remains constant over a wide pO2 range. The critical pO2 is raised to 10
mm Hg. Below this pO2 the oxygen consumption decreases with
decreasing available oxygen. Using the Oxystat system it could thus be
shown that stimulation not only produces an increase in oxygen consumption but also that the critical oxygen value, below which the cells
are in oxygen deficit, is displaced towards higher partial O2 pressures.
$
BS4 73-0117
BS4 73-0120
2,219.00 PO2 Electrode Type 2000-100
BS4 73-0122
7,203.00 Oxystat Measuring System Controller Model 719,
Includes Controller and to Connect pO2 Electrode;
with Recording Outputs for pO2, pO2 Drift and
Volume, 115/230 VAC, 60/50 Hz
BS4 73-0124
5,518.00 Square Wave Stimulator G270, for Biphasic
Stimulation, 115/230 VAC, 60/50 Hz
BS4 50-1932
2,900.00 Harvard Apparatus Thermocirculator, 115 VAC, 60 Hz
BS4 50-1940
2,900.00 Harvard Apparatus Thermocirculator, 230 VAC, 50 Hz
Cell Biology
Fig. 3 shows an original recording. Isolated cardiomyocytes were incubated at a pO2 of 90 mm Hg and seven samples were withdrawn consecutively for biochemical analysis. Curve 2 is the record of the pO2
electrode. The electrode was calibrated with aerated solution (155
mm Hg). After the cells were added, the pO2 in the chamber drops
steeply. When the pO2 falls below 90 mm Hg the controller starts the
pump and aerated solution is added (curve 1) until the pO2 has
returned to its set value. The pump is operated more or less frequently depending on the O2 consumption of the cells. From the solution
volume added in unit time, i.e. the slope of curve 1, it is possible to
calculate the oxygen consumption of the isolated cardiomyocytes. After
taking the samples (0.8 ml each, arrows) the total quantity of cells in
the chamber is reduced and less solution is required in order to maintain the chamber pO2 at 90 mm Hg. The slope of curve 1 is correspondingly reduced.
Figure 4: Oxygen consumption (VO2) of isolated cardiomyocytes at different
values of available oxygen.
Optional Accessories
BS4 72-2987
BS4 73-1523
BS4 73-0210
2,648.00 2-Channel Flatbed Recorder, Economy Version,
250 mm Chart Width
629.00 Plugsys Minicase
2,380.00 OPPM Oxygen Partial Pressure Amplifier Module
For the direct detection of oxygen, see page M6.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M17
Respirometry
Precision Dissolved Oxygen Measurement and Respirometry
Specialized Tools For Bioresearch
Strathkelvin Instruments dissolved oxygen respirometry systems are based on ultra precise microcathode oxygen electrodes. The oxygen
meters and interfaces are supplied with dedicated respirometry software. A choice of respirometer cells, with volumes from 50 microlitres
to 3 ml, are available to meet most biomedical research applications.
NEW 6-Channel Oxygen System Model 928
The 928 is a complete system for replicate
measurements of dissolved oxygen, together
with data recording and analysis by computer. Use of this system will result in consider• Flow
able time saving in both respiration experirespirometry
ments and in dissolved oxygen monitoring
• Dissolved
situations. The system is comprised of a
oxygen
microprocessor-based interface which
monitoring
accepts up to six 1302 microcathode oxygen
electrodes, and dedicated Windows software.
All operations are controlled from the computer screen, and output
of recordings in chart recorder format to a printer is available.
•
For closed
chamber
respirometry
The interface provides the polarizing voltage to the electrodes,
amplifies the low-level signals, makes the A/D conversion, and feeds
the resulting data stream to the computer. The system is a “virtual
instrument” therefore there are no front panel controls. All operations are controlled by the computer. An illuminated front panel display provides information on the activity of the interface and displays
readings from the electrodes when they are in use.
The 928 Oxygen System may be used with the Model RC 650 6-electrode Respirometer, see page M21. Details of the software are shown
on the facing page. Software included.
Specifications
NEW 1-2 Channel Oxygen Meter
This versatile new Oxygen Meter
acts as both a stand alone oxygen meter, displaying and storing oxygen values, or as an
• Interfaces to respirometry/monitoring software,
interface to respirometry or
or use as stand alone
monitoring software running on
meter
a PC. It accepts inputs from
• Use any unit of PO2 or
either one or two Strathkelvin
oxygen concentration
1302 microcathode oxygen
• Closed cell, flowelectrodes, and shows the oxythrough respirometry
and O2 monitoring
gen values on a large liquid
software included
crystal display. The display will
show units of either PO2 (mm
Hg; torr; KPa) or concentration (µmol/l; µg/l; mg/l; µl/ml; ml/l;
ppm; or % saturation). When connected to the software (supplied,
which is installed on a PC, it can be used for either closed cell
respirometry (using the MT200 mitocell; RC300 or RC350 respiration cell) or for flow respirometry (using the FC100 flowcell or
MC100 microcell). Alternatively the software can be used to monitor
dissolved oxygen, as in isolated organ preparations. Details of the
software are shown on the facing page. Software included.
•
Operates with 1 or 2
oxygen electrodes
Specifications
Resolution
0.01% of full scale
Repeatability
0.01%
Resolution with 1302 Electrode
0.1% of air-saturated water
Logging Capacity
2000 readings
Polarizing Voltage
400-900 mV(anode connected to ground)
Computer Interface
USB1
Power Requirements
100 to 250 VAC, 50 or 60 Hz
Power Requirements
Dimensions
275 x 258 x 117 mm (11 x 11 x 4.5 in)
5V, 1A from external plugtop power supply, 100-230 VAC,
47-63 Hz
Dimensions
185 x 135 x 105 mm (7.25 x 5.25 x 4.125 in)
Catalog No.
BS4 69-3000
$
Model
8,867.00 928
Product
6-Channel Oxygen System
Catalog No.
BS4 69-3001
M18
$
Model
3,119.00 782
Product
1-2 Channel Oxygen Meter
11,000 Specialty Products to Enhance Your Bioresearch
Respirometry
NEW
Respirometry Software
The software used in the 782 meter and the 928 system is essentially similar. The main difference is that the 928 displays up to 6 traces,
while the 782 has a maximum of two.
The program has been written so that it is extremely user-friendly
and quick to learn. It can be used for closed cell respirometry (with
the MT200, RC300, RC350 or RC650), or flow respirometry (using
the FC100 or MC100) or for monitoring dissolved oxygen (using the
FC 100 or MC100). When used for respirometry experiments, it follows the familiar logical sequences of operations, from electrode
calibration to recording and analysis. The program follows a
sequence of 3 main stages: Setting up, Recording, and Analysis.
Setup
The setup screen is used for entering all of the details which the computer needs in order to record in the correct units, and to express
the respiration rate, normalized to biomass units if required, in the
desired units. All experimental details entered here are transferred
through to the final calculated results report page. During setup, the
cell volumes (for closed cell) and flow rate, electrode positioning
(for flow respirometry), temperature of the experiment, scrolling
speed of the traces during recording, and the instruction to print the
recording screen to printer (if hard copy is required) are all
entered. Many of these will not change from one experiment to the
next, and it is only necessary to enter changes.
Analysis
The data file, recorded traces, and event marker flags are recalled to
the screen. If the recording has exceeded one screen width, it can be
contracted to fit the screen, if required. Alternatively it is possible to
scroll along long recording traces. Selector lines may be dragged to
enclose specific parts of the trace. Thus if the traces show both control respiration and changed respiration after adding a solution, the
selector lines would be dragged to the control rate first and then to
the changed rates.
When the software is used for dissolved oxygen monitoring, the
Analysis screen which displays the recorded traces and the oxygen
values at flagged points or at set times can be transferred to the
Results and report page.
The program requires a computer with a minimum specification of:
Pentium processor, 1 free serial port, 64 Mb RAM, Windows 95 or
later and 4 MB hard disk space. A comprehensive Instruction
Manual is supplied, which will be particularly valuable to anyone
who is not familiar with respirometric work.
This software is supplied with the 6-Channel Oxygen System Model
928 and the 1-2 Channel Oxygen Meter. It is not available separately.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
Cell Biology
Recording
Clicking the ‘Start’ button initiates the recording process, and traces
then scroll across the screen. If substrates, inhibitors or other solutions require to be added during the run, the position where this
occurs can be recorded with a flag, and the nature of the addition is
recorded on a drop down menu. At the termination of the experiment, the data are saved to a data file, and may be analyzed straight
away if required.
In each case respiration rates (normalized if so designated) are calculated automatically by clicking the ‘Calculate’ button. The calculated rates are tabulated, together with the experimental details entered
during Setup, on the report page. The results may be exported to a
spreadsheet, word processing or statistics package. The Analysis
screen and the Report page may be printed, if required.
M19
Respirometry
Specialized Tools For Bioresearch
NEW
Microcathode Oxygen
Electrode Model 1302
This is a Clark-type
polarographic electrode with a 22
• High stability of output
micron diameter
platinum cathode
• Very low rate of oxygen consumption
and
silver/silver
• Compatibility with all Strathkelvin
chloride anode conaccessories
nected by a buffered
potassium chloride
electrolyte solution. In the normal configuration the cathode is covered with a relatively low permeability polypropylene membrane, in
order for the electrode to be used in unstirred solutions or where
minimal stirring is required. This membrane gives a relatively slow
response time. For fast response, as required for rapidly respiring
enzyme preparations, a thin FEP membrane (used with a special
electrode jacket, (BS4 69-3011) is used. Rapid stirring of the medium is then necessary. The electrodes are not temperature compensated and require controlled temperature environments (within
0.1°C). They should always be used in the electrode holder of one of
the Strathkelvin accessories, so that only the tip of the electrode is
exposed to the medium.
•
Mitocell Miniature
Respirometer Model MT200
and MT200A
May be used with or without stirring
the medium
Specifications
Response Time at 37°C:
Polypropylene Membranes
FEP Membranes
c 18 sec for 90% change
c 6 sec for 90% change
Oxygen Consumption (Polypropylene Membranes)
c 0.5 - 3.0 x 10-10 mg O2/min
Temperature Coefficient
c 2% per °C
Catalog No.
M20
NEW
$
Model
BS4 69-3006
854.00
1302
Product
BS4 69-3007
131.00
–
Oxygen Electrode Service Kit
Microcathode Oxygen Electrode
•
Miniature respirometer for mitochondria and cell suspensions
•
Integral magnetic stirrer
•
Glass chamber with volumes of 50/100 µl (MT200) or
0.3/0.5/1.0 ml (MT200A), and good visibility of contents
•
Transparent polycarbonate plunger
•
Substrate and inhibitors may be injected directly into chamber
This new Mitocell Miniature Respirometer has a chamber volume of
only 50 µl and was introduced for measurements on mitochondria
isolated from biopsy samples. It can be used in any situation where
sample size is limited. The base section contains an integral solid
state, fixed speed magnetic stirrer. The 1302 microcathode electrode
(BS4 69-3006) is inserted from beneath the unit, and its projecting
tip forms the base of the respirometer chamber. The glass chamber
unit is surrounded by a water jacket through which constant temperature water is circulated. The respiration cell has a polycarbonate
plunger with a central capillary through which solutions may be
added during the course of a respiration run. Stainless steel magnetic spinbars are supplied together with a special 1 µl syringe supplied
with MT200 and a 5 µl syringe with the MT200A for solution additions. An alternative version with a large volume (0.3, 0.5 and
1.0 ml) and a PTFE coated stirbar is also available.
BS4 69-3008
27.00
–
Electrolyte Solution, 125 ml
Catalog No.
BS4 69-3009
30.00
–
Polypropylene Membranes, Box of 15
BS4 69-3033
BS4 69-3010
30.00
–
FEP Membranes, pkg. of 15
BS4 69-3011
27.00
–
Electrode Jacket (with Hole) for FEP
Membranes
BS4 69-3012
27.00
–
BS4 69-3013
30.00
–
$
Model
Product
1,091.00
MT200
Mitocell Miniature Respirometer,
50/100 µl
BS4 72-6186
999.00 MT200A
Mitocell Miniature Respirometer,
0.3/0.5/1.0 ml
Electrode Jacket (without Hole) for
Polypropylene Membranes
BS4 72-6187
295.00
SK205
Replacement Glass Plunger for
MT200
O-Rings, Box of 25 SI130
BS4 72-6188
295.00
SK206
Replacement Glass Plunger for
MT200A
11,000 Specialty Products to Enhance Your Bioresearch
Respirometry
NEW
6 Electrode Respirometer
Model RC 650
Ability to carry out
replicate experiments,
utilizing the advanced
recording and analysis
features of the Strathkelvin\928 system
Specifications
16 mm
Volume of Cells
1 to 3 ml (adjustable)
Cell
Precision bore glass
Electrode Holder
Black acetal, with peek tip
Clear and black acrylic
Magnetic Stirrer
Catalog No.
BS4 69-3021
6-position; 12 VDC from external plugtop power supply,
100-230 V, 47-63 Hz
$
Model
3,722.00 RC 650
These respiration cells are for
small volume measurements of
• Good visibility of cell
oxygen uptake rate by microbial
contents
suspensions, cell suspensions,
mitochondria or enzyme preparations. The electrode holder (supplied with the cell) slides into one
of two glass tubes fused into the glass water jacket. The second tube
is used to park the electrode holder when changing solutions. A fine
slot (1 x 1 mm) in the side of the holder allows air to escape when
the holder is inserted into the cell. It may also be used to introduce
other solutions during the course of a respiration run and a fine bore
needle with luer fitting (included). A PTFE covered magnetic spinbar
is provided and the cell should be located on a magnetic stirrer unit
(not provided). For fast reactions, it is recommended that FEP membranes used with electrode jacket (BS4 69-3011) are used.
•
Small sample volume
Specifications
Model
RC 300
RC 350
Cell Diameter
13 mm
16 mm
Cell Volume
0.3 to 1.0 ml
1.0 to 3.0 ml
Cell
Glass
Electrode Holder
Black acetal
Catalog No.
Diameter of Cells
Bath
Respiration Cells RC 300
and RC 350
$
Model
Cell Biology
For use with the 928 6 channel oxygen
system - for replicate respiration
measurements of cell suspensions,
microbial suspensions, mitochondria
or enzyme preparations The
respirometer consists of a sealed
water both containing six respirome• Integral unit complete
with magnetic stirrer
ter cells located above a six-position
magnetic stirrer. Electrode holders,
• Good visibility of cell
contents
with a hole in their tips through which
the membrane of the 1302 electrode
• Variable cell volume
protrudes, make a sliding fit into the
cells. As the holder is inserted into the
preparation, the sloping face at its tip gathers the air into the slot, which
runs the complete length of the holder. Respiratory inhibitors or other
solutions can be injected directly into the cell via this slot, using a fine
bore needle (included). The volume of the cells may be set between 1
and 3 ml, by rotating the knurled collar of the holders. The rear of the
bath contains a further 6 cells which are used to park the electrode holders and maintain them at a constant temperature while changing the contents of the respiratory cells. For easy removal and cleaning the cells are
sealed into the water bath by O-rings.
•
NEW
Product
BS4 69-3018
602.00 RC 300
Respiration Cell
BS4 69-3019
662.00 RC 350
Respiration Cell
BS4 69-3027
285.00
–
Replacement Glass for BS4 69-3018
BS4 69-3028
290.00
–
Replacement Glass for BS4 69-3019
BS4 69-3032
24.00
–
Spinal Needles for use with BS4 693018 & BS4 69-3019, pkg. of 3
Product
6 Electrode Respirometer
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M21
Respirometry
Specialized Tools For Bioresearch
NEW
MicroCell Model MC 100
•
For pO2 measurement of
blood or other fluids, or
for micro flowcell use
•
Small Sample Volume
•
Flow through or spot
measurement
•
Exceptional visibility
•
Easy acess for cleaning
The all-glass sample chamber has
a capacity of approximately 70µl.
Samples are injected into the
chamber through a 1mm stainless steel tube fitted with a fine
p.v.c needle-locating collar.
Alternatively, the cell can be used as a micro flowcell by connecting the
outflow from a respiring preparation. The sample leaves the chamber
through a glass Luer fitting to which a small bore flexible drainage tube
can be connected. The sample chamber is completely surrounded by
water circulated through the glass water jacket from a constant temperature bath. This provides good temperature regulation while providing
excellent visibility of the contents of the chamber. The water jacket
screws into an acetal base section and can be easily removed for cleaning. The microcathode electrode fits into the central electrode holder
and the membrane at its tip forms the floor of the sample chamber.
NEW
Flow Cell FC 100
•
For continuous in-line
oxygen monitoring or for
connection to flow-through
respirometer chamber
•
Easily disassembled for
cleaning
•
Good visibility of flowline
The acrylic chamber of the
flowcell screws onto the electrode holder. Only the tip of the
electrode projects into the water flow. The inlet and outlet tubes are
made from stainless steel. The electrode holder is fitted with a collar for attachment to a vertical or horizontal (1/4 to 3/8 inch)
diameter laboratory rod to enable the flowcell to be immersed
below the surface of the constant temperature bath containing the
respirometer chamber.
Specifications
Material:
Electrode Holder
Collar
Chamber
Black acetal
Black acetal
Clear acrylic
Inlet/Outlet
6 mm OD and 3 mm ID stainless steel
Catalog No.
BS4 69-3023
$
Model
328.00 FC 100
Product
Flow Cell
Specifications
Sample Chamber
70 µl
Overall Height
106 mm
Jacket and Cell
Glass
Base Section
Black acetal
Catalog No.
BS4 69-3016
$
NEW
Model
919.00 MC 100
General Electrode Holder
EH 100
Product
•
MicroCell Model
For use with any of your
own custom respiration
chamber designs
The electrode holder is a black
acetal tube in which the electrode
is located so that its tip projects
from the end. The holder is used
for the insertion of the electrode
into your own respiration chamber. An acrylic collar containing an O-ring
on its inside surface is also provided. This can be cemented over a 13mm
hole drilled in the wall of the respiration chamber to facilitate insertion of
the electrode holder. The electrode should only be used in an electrode
holder, to avoid ground- leak damage.
Specifications
Electrode Holder
Acrylic Collar
Dimensions
13 x 85 mm (D x L)
26 x 10 mm (D x L)
Holder Material
Black acetal
Clear acetal
Catalog No.
BS4 69-3024
M22
$
Model
219.00 EH 100
Product
Electrode Holder
11,000 Specialty Products to Enhance Your Bioresearch
pH and Reference Electrodes
Microminiature CO2 and pH Electrodes
This series of micro pH, O2 and CO2 electrodes has been specifically
designed for use in various area of bioscience research. Their microminiature design and small sensing area allows them to be used
for small area measurements. This series of pH electrodes have similar response characteristics to standard size, glass pH electrodes
making them compatible with any standard pH meter. The pH series
of electrodes come in a variety of configurations to fit every need.
Micro-CO2 Electrode
Single pH Electrode in 25 Gauge Needle
This is a microminiature pH electrode mounted in the lumen of a fine
hypodermic needle. This configuration allows for easy penetration and
insertion in all types of tissue for on-line/real time monitoring of pH.
This pH electrode does not contain an external reference. To use this
electrode and external reference electrode such as the BS4 69-0490 or
similar reference electrode must be used. The electrode is stored dry.
Specifications
This CO2 electrode is connected to the BNC connector of a standard
pH meter by developing a calibration curve % CO2 vs. mV using the mV
mode on the pH meter. The calibrations curve can be generated by
using two known concentration standards.
Response Time
~15 sec
Slope
55 mV per pH, range of 1 to 14
Impedance
1500 M Ω
Stability
0.05 pH per day
Catalog No.
Catalog No.
BS4 69-0499
$
$
Product
Product
BS4 69-0491
280.00 Single pH Electrode in 25 ga Needle
424.00 Micro-CO2 Electrode
BS4 69-0490
180.00 Flexible Reference Electrode, see page M28.
Combination pH Electrode in 20 Gauge Needle
This a miniature combination pH/reference electrode has been
designed to fit small perfusion chambers without effecting fluid
exchange rates. It does not require an external reference electrode.
The reference solution can be replaced. The electrode is stored wet.
This is a miniature combination pH/reference electrode that is mounted in a 20 gauge, 1 inch x 0.5 mm OD stainless steel tube. The stainless steel tube provides protection to damage of the internal miniature
glass pH sensor. It does not require an external reference electrode.
The reference solution can be replaced. The electrode is stored wet.
Tip length is 1.8 mm.
Specifications
Response Time
~10 sec
Slope
55 mV per pH, range of 1 to 14
Specifications
Impedance
200 M Ω
Response Time
~10 sec
Stability
0.05 pH per day
Slope
55 mV per pH, range of 1 to 14
Dimensions, L x D
38.1 x 0.75 mm (1.5 x 0.03 in)
Impedance
200 M Ω
Stability
0.05 pH per day
Dimensions, L x D
25.4 x 0.9 mm (1 x 0.035 in)
Catalog No.
BS4 69-0494
$
Product
Cell Biology
Combination pH Electrode for Micro Wells
424.00 Combination pH Electrode for Micro Wells
Catalog No.
BS4 69-0493
$
Product
424.00 Combination pH Electrode in 20 ga Needle
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M23
pH and Reference Electrodes
AMANI ‘LEAK FREE’ Microcombination pH Electrodes
‘LEAK FREE’
AMANI 650, 1000, 1000L Series pH Sensor
‘Duck Head’
•
Integrated ‘LEAK-FREE’ non-porous
reference junction eliminating problems of clogged junction and absorption of proteins and surfactants
•
All plastic construction, unbreakable
and tough — no glass!
•
Can be used with any pH meter with
BNC connector
•
‘Zero’ depth of immersion, just touch
sample to flat tip
•
Superior chemical resistant — can
be even be used in hydrofluoric acid
solutions
•
Tip diameter as small as 650 µm
including reference electrode
•
Standard Nernstian behavior
•
Flexible version is available for use
in restricted areas such as microscope stage
•
Long working life
•
Low cost
•
Maintenance-free, dry or wet
storage, no filling solutions needed
Specialized Tools For Bioresearch
AMANI IV 18.5 ga “Duck Head” Stainless Steel Needle
M24
The AMANI ‘LEAK FREE’ series of non-glass, micro pH electrodes are the latest development in
our combination (built in reference) pH electrode line. The new technologies used in these
electrodes provide superior sensitivity and stability. The ‘LEAK FREE’ non-porous reference
junction eliminates the problems of clogged junction and adsorption of proteins and surfactants. They also have high resistance to chemical including hydrofluoric acid solutions.
With tip diameters starting as low as 650 µm, they are one of the smallest combinations pH
electrodes on the market today. The new ‘DUCK HEAD’ design of the AMANI IV has been developed to offer complete protection for the electrode’s tip during insertion without compromising the electrodes performance. The electrode is built into a custom 18.5 ga (1.2 mm) needle and can be used to measure the pH of solid samples such meats, fruits, and vegetables
without sample preparation. Custom configurations of the AMANI series of pH combination
electrodes are also available, please contact Harvard Apparatus Technical Sales Department
for further details.
Specifications
Model
AMANI-650
AMANI-1000
AMANI-1000L
AMANI-IV
Tip Diameter
650 µm
1000 µm
1000 µm
1200 µm
Tip Length
20 mm (0.8 in)
20 mm (0.8 in)
75 mm (3 in)
37 mm (1.5 in)
Construction
All Plastic
All Plastic
All Plastic
Plastic/Stainless Steel
Depth of Immersion
< 100 µm
< 100 µm
< 100 µm
1000 µm
Min. Sample Volume
250 nL
500 nL
500 nL
n/a
Response Time
< 3 sec, 90% response < 3 sec, 90% response < 3 sec, 90% response < 3 sec, 90% response
Temperature Range
0° to 100°C
0° to 100°C
0° to 100°C
0° to 100°C
Internal Reference
Ag/AgCl
Ag/AgCl
Ag/AgCl
Ag/AgCl
Slope
Nernstian
Nernstian
Nernstian
Nernstian
pH Range
0 to 14
0 to 14
0 to 14
0 to 14
Storage
Wet or dry
Wet or dry
Wet or dry
Wet or dry
Connector
BNC
BNC
BNC
BNC
Electrode Holder
OD x Length
4.8 x 75 mm
(0.19 x 2.9 in)
4.8 x 75 mm
(0.19 x 2.9 in)
4.5 x 50 mm
(0.18 x 2 in)
4.8 x 50 mm
(0.19 x 2 in)
Lead Wire
0.9 m (3 ft)
0.9 m (3 ft)
0.9 m (3 ft)
0.9 m (3 ft)
Catalog No.
$
BS4 69-0020
383.00
BS4 69-0021
355.00
BS4 69-0022
383.00
BS4 69-0019
437.00
Harvard Apparatus
sells a complete line
of pH Meters and
Magnetic Stirrers.
See the General
Laboratory Section O
or visit our website
at www.harvardapparatus.com.
11,000 Specialty Products to Enhance Your Bioresearch
pH and Reference Electrodes
Microminiature pH Electrodes
Combination pH Electrode in 18 Gauge SS Tube
Combination Gastroesophageal pH Electrode
This miniature combination pH/reference electrode is mounted in an
18 gauge, 3 inch x 1.25 mm OD stainless steel tube. The stainless steel
tube provides protection to damage of the internal miniature glass pH
sensor. It does not require an external reference electrode. The reference solution can be replaced. The electrode is stored wet. Tip length
is 1.5 mm.
This miniature combination pH/reference electrode has a long 3 foot
long flexible tube to fit into the esophagus. It does not require an
external reference electrode. The reference solution can be replaced.
The electrode is stored wet.
Specifications
Specifications
Response Time
~10 sec
Slope
55 mV per pH, range of 1 to 14
Response Time
~10 sec
Impedance
200 M Ω
Slope
55 mV per pH, range of 1 to 14
Stability
0.05 pH per day
Impedance
200 M Ω
Electrode OD
1.6 mm
Stability
0.05 pH per day
Dimensions, L x D
91.4 cm x 1.6 mm (3 ft x 0.06 in)
Dimensions, L x D
76 x 1.25 mm (3 x 0.005 in)
Catalog No.
BS4 69-0492
$
Product
$
Product
424.00 Combination Gastroesophageal pH Electrode
Specifications
Response Time
~10 sec
Slope
55 mV per pH, range of 1 to 14
Impedance
200 M Ω
Stability
0.05 pH per day
Dimensions, L x D
31.7 cm x 3 mm (1.25 x 0.1 in)
$
Product
Flexible Combination pH Electrode
for Free Flap Surgery
Cell Biology
This miniature combination pH/reference electrode is mounted in an
11 gauge 1.25 inch x 3 mm OD stainless steel tube. The stainless steel
tube provides protection to damage of the internal miniature glass pH
sensor. It does not require an external reference electrode. The reference solution can be replaced. The electrode is stored wet.
BS4 69-0497
BS4 69-0495
424.00 Combination pH Electrode in 18 ga SS Tube, Model
IC-201
Combination pH Electrode
in 11 Gauge SS Tube
Catalog No.
Catalog No.
This miniature combination pH/reference electrode has been specifically designed to be used in free flap surgery. It does not require an
external reference electrode. The reference solution can be replaced.
The electrode is stored wet.
Specifications
Response Time
~10 sec
Slope
55 mV per pH, range of 1 to 14
Impedance
200 M Ω
Stability
0.05 pH per day
Dimensions, L x D
91.4 x 1.6 mm (3 x 0.06 in)
Catalog No.
BS4 69-0496
$
Product
424.00 Combination pH Electrode for Free Flap Surgery
424.00 Combination pH Electrode in 11 ga SS Tube
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M25
pH and Reference Electrodes
Protein Resistant pH Electrodes
Minitrode™ P
Minitrode™ P
Freezetrode™
Specialized Tools For Bioresearch
Flushtrode™ P
Tiptrode™
Spintrode™ P
The small size of this sensor makes it ideal for pH measurement of
sample in microplates and microcentrifuge tubes. The sensor tip is
3 mm diameter with a usable length of 100 mm (3.9 inches).
Freezetrode™
This sensor is ideal for measuring the pH of samples stored in cold
rooms. Select this sensor if your sample is between -20° to +80°C. It
has a 12 mm diameter tip.
Flushtrode™ P
If your sample contains between 5 to 85% water, this is the ideal sensor for you. It is good for determining pH of buffer solutions. It has a
12 mm diameter tip.
Tiptrode™
This pH sensor was designed for pH measurements in applications
requiring penetration of solid and semi-solid materials including:
foods, gelatinous materials, and viscous buffers. It has a 6 mm tip.
Spintrode™ P
Use the SPINTRODE™ to determine the pH of samples in NMR tubes.
The 3 mm diameter, 180 mm (7 inch) long sensor allows you to determine the pH without transferring the sample from the NMR tube.
•
Ideal for determining
pH in samples that
contain protein
•
Faster response times
than traditional pH
electrodes
•
More accurate than traditional pH electrodes
•
Longer electrode life
— more economical
•
Five styles available to
meet most application
needs
•
Measuring the pH of biological
samples containing proteins is difficult. Proteins in solution can combine with silver ions from the electrolyte to clog the pH electrode
diaphragm. This leads to long
response times, inaccurate pH
measurements, and eventually discarded electrodes. These protein
resistant electrodes are designed
for rapid, accurate, and reproducible pH measurements of protein containing solutions.
pH range from 2 to 11
Unique to these pH electrodes is
the
specially
formulated
Protelyte™ reference electrolyte. Protelyte™ electrolyte prevents the
precipitation of proteins on the electrode diaphragm. You get faster,
more accurate, reproducible pH measurements of your samples.
Select from five specialized electrodes. The best electrode for you
depends upon your applications, physical size limitations, and temperature range. Each has a pH range of 2 to 11.
These pH Electrodes are supplied individually and have S7 type connectors. To ensure the most accurate pH measurements, calibrate your
electrodes with DURACAL™ Buffer Solutions, see next page for more
information.
Catalog No.
$
BS4 69-0075
27.00 Protelyte™ Reference Electrolyte Solution, 100 ml
BS4 69-0076
59.00 Electrode Storage Solution, 500 ml
BS4 69-0077
27.00 Electrode Connecting Cable, BNC to AS7, 1 m (3.3 ft)
BS4 69-0078
Product
51.00 Electrode Connecting Cable, DIN to AS7, 1 m (3.3 ft)
BS4 69-0079
110.00 Electrode Connecting Cable, Metrohm (Lemo) to
AS7, Type F, 1 m (3.3 ft)
BS4 69-0080
110.00 Electrode Connecting Cable, Metrohm (Lemo) to
AS7, Type H, 1 m (3.3 ft)
BS4 69-0081
85.00 Electrode Connecting Cable, Radiometer to AS7,
1 m (3.3 ft)
BS4 69-0082
59.00 Electrode Connecting Cable, Standard US to AS7,
1 m (3.3 ft)
Specifications
Catalog No.
BS4 69-0070
$
271.00
BS4 69-0071
BS4 69-0072
198.00
BS4 69-0073
BS4 69-0074
220.00
Outer
Electrode
Length
Diameter
Temp. Range
Minitrode™ P
100 mm
3 mm
0° to 60°C
Spintrode™ P
180 mm
3 mm
0° to 60°C
Freezetrode™
120 mm
12 mm
-20°C to 80°C
Flushtrode™ P
120 mm
12 mm
10° to 50°C
Tiptrode
25 mm
6 mm
0° to 80°C
™
For DURACAL™ Buffer Solutions, see page M27
For the AMANI series of ‘LEAK-FREE’ pH
Electrodes, see pages M24
For Microminiature pH Electrodes, O2 Electrodes
and CO2 Electrodes, see pages M23 and M25
M26
11,000 Specialty Products to Enhance Your Bioresearch
pH and Reference Electrodes
DURACAL™ Buffers
Accurate, stable buffers are critical for the calibration of pH and
redox electrodes to achieve accurate measurements, particularly with
modern pH meters incorporating GLP functions for recording electrode performance. DURACAL™ pH and Redox Buffer solutions are
the buffers you can trust. They offer you many advantages over traditional buffer solutions: guaranteed five year stability, excellent stability of even pH 9.21 in air, see Figure 1, automatic buffer recognition
so you use the correct buffer to calibrate. All of this in a unique time
saving, money saving dispensing bottle.
•
Stable, guaranteed 5 year buffer
lifetime
• Unique time saving CALPACK™
dosing bottle
— No more searching for suitable
containers
— No more cleaning
— No more risk of contamination
• Economical and easy to dispense
only the required quantity of buffer
for calibration, no wasted buffer
• Automatic buffer recognition with a
majority of pH meters
• Certified, traceable to N.I.S.T.
DURACAL Buffers are guaranteed for 5 years from the date of production. During this time they retain a high buffer capacity for fast, stable
calibrations. The pH 7.00 buffer is less sensitive to ions while the pH
9.21 buffer provides stability superior to conventional buffers. See Figure 1 for a comparison of
DURACAL™ and conventional buffer stability when subjected to prolonged air exposure.
The unique, easy-to-use, CALPACK™ dispensing bottle allow electrode calibration in the buffer bottle without contamination. In 4 easy steps, calibration is complete, see Figure 2. It’s that simple!
No more searching for a suitable container, no more cleaning, no more risk of contamination,
and no more wasted buffer from overfilling a container. The composition of DURACAL™ Buffers
has been optimized so the temperature function is similar to that of conventional buffers. This
guarantees that the automatic buffer recognition of most pH meters can be used without any
problems.
Duracal™ Buffers
pH 4.01
pH 7.00
pH 9.21
pH 10.01
pH
±0.02
±0.02
±0.02
±0.02
±5 mV
Solution Color
Red
Green
Blue
Yellow
Yellow
1 Bottle, 500 ml
$
BS4 69-0083
34.00
BS4 69-0084
22.00
BS4 69-0085
34.00
BS4 69-0086
34.00
BS4 72-7140
58.00
Multipack (500 ml x 3) BS4 69-0088
$
BS4 69-0089
BS4 69-0090
BS4 69-0091
–
Catalog No.
$
BS4 69-0092
81.00 Buffer Set, One 500 ml Bottle of 4.01, 7.00 and 9.21
BS4 72-7141
58.00 Buffer Set, One 500 ml Bottle of 4.01, 7.00 and 10.01
Redox +475 mV
Product
Cell Biology
Specifications
Figure 1
Step 1. Unscrew cap to access
calibration compartment.
Step 2. Squeeze CALPAK™ bottle to
force buffer into compartment
Step 3. Insert electrode for calibration.
Step 4. Turn CALPAK™ bottle on its
side to dispose of used buffer.
Figure 2: Calibrating Electrodes in the CALPAK™ Dosing Bottle
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M27
Reference Electrodes
Reference Electrodes
through the junction in either direction. The reference is totally leak free!
The junction is resistant to most commonly used organic solvents. It
offers excellent resistance to acetonitrile, DMSO, THF, MEK, acetone,
dichloromethane, esters, alcohols, and ketones, hydrofluoric acid or
common dilute acids and bases. Unlike conventional reference electrodes, there is no need to store them in concentrated chloride solution. If the electrode is left dry for a long period of time, it should be
immersed in water for few hours to recover.
BS4 69-0025
Two sizes of ‘LEAK FREE’ reference electrodes are available, see
ordering chart below for details. Other custom configuration of the
leak-free reference electrode are available, please consult with
Harvard Apparatus Technical Sales Department.
BS4 69-0024
Low-Leakage Reference Electrodes
Specialized Tools For Bioresearch
BS4 69-0023
BS4 69-0053
LEAK-FREE Reference Electrodes
The ‘LEAK FREE’ reference electrodes were developed to eliminate the
problems associated with the use of conventional porous junction-based
reference electrodes such as clogging, sample contamination, electrolyte
loss, changing of ionic strength of the sample, and the difficulties with
organic based solvents. They utilize a unique junction that is highly conductive (< 10 K Ω) but not porous. There is no solution migrating
The low-leakage reference electrodes are for use in applications where
contamination would interfere with the application. The electrode junctions are resistant to most commonly used organic solvents. They offer
excellent resistance to acetonitrile, DMSO, THF, MEK, acetone,
dichloromethane, esters, alcohols, and ketones. Hydrofluoric acid or
common dilute acids and bases have no effect on the on these electrodes.
Two low-leakage reference electrodes are available. One is 2 mm (0.08
in) in diameter and the second is 5 mm (0.2 in) in diameter. Other custom configurations of the low-leakage reference electrodes are available,
please call the Harvard Apparatus Technical Sales Department.
Specifications
Diameter
Length
Construction
Leakage Rate
Filling Electrolyte
Electrical Connection
Junction Resistance
Temperature Range
Storage
Catalog No.
$
Low-Leakage Reference Electrodes
2 mm (0.08 in)
5 mm (0.2 in)
130 mm (5.1 in)
130 mm (5.1 in)
PEEK*
PEEK*
< 5 x 10-12 l/min
5 x 10-12 l/min
3 M KCl
3 M KCl
2 mm pin on 30 in lead
2 mm pin
< 2.5 K Ω†
< 1 K Ω†
5° to 80°C
5° to 80°C
3 M KCl
3 M KCl
BS4 69-0024
BS4 69-0025
LEAK-FREE Reference Electrodes
1 mm (0.04 in)
2 mm (0.08 in)
100 mm (3.9 in)
65 mm (2.6 in)
PEEK*
PEEK*
0
0
3 M KCl
3 M KCl
1 mm gold plated pin
1 mm gold plated pin
< 10 K Ω†
< 10 K Ω†
5° to 80°C
5° to 80°C
Distilled water
Distilled water
BS4 69-0053
BS4 69-0023
* Polyetherether Ketone
† In 1 M potassium chloride
Flexible Reference Electrode
This miniature flexible reference electrode is 3 inches long and has
an outside diameter of 2 mm. It comes complete with a 4 foot long
cable and is terminated with an alligator clip connector. Each unit
comes pre-filled with the reference solution 3.0 M NaCl in 25%
Glycerol) and it is stored dry. If refilling is ever required, reference
solution can be injection into the side of the tee fitting and flexible
tube portion of the electrode.
Catalog No.
BS4 69-0490
$
Product
180.00 Flexible Reference Electrode
For Ag-AgCl Reference Electrodes,
see pages M30 and M31.
M28
11,000 Specialty Products to Enhance Your Bioresearch
Reference Electrodes
Auto Chloridizer 863 Kit
Microelectrode Starter Kit
•
Simple means to
chloridize Ag/AgCl
electrodes
•
Environmentally safe
The electrolytic chloridation unit
Auto Chloridizer 863 provides a
simple means for the chloridation
of Ag/AgCl electrodes. It covers
the surface of the silver electrode
with an AgCl film insoluble in
water. Unlike a purely chemical
procedure, electrolytic chloridation does not involve the use of substances endangering the environment. It is normal practice to work with physiological saline solution
(NaCl) as the electrolyte.
The electrolytic chloridation method also offers excellent reproducibility of the AgCl
film at constant current density and chloridation time. The auto chloridizer operates
with an electronically controlled constant-current source with current monitoring
and error indication through an LED and error beep. During the chloridation of a
single Ag/AgCl electrode the unit is operated manually through the start/stop key.
The counter electrode (cathode) used is a carbon-glass ceramic crucible. This
material has a very high electrical conductivity, is very largely inert physiologically,
and resistant to oxidation and corrosion. There is therefore no contamination of
the AgCl film through free metal ions, as is the case when using stainless steel as a
cathode for example.
Specifications
Chloridation Current
Electronically controlled constant current source with current monitoring facility,
and with low/medium/high selection by switch, corresponding to approx. 5/10/15
mA respectively
Chloridation Voltage
Max. driving voltage of constant current source is approx. 15 V and is therefore
completely safe for user
Start/Stop
Manual operating mode start/stop for chloridation of single electrodes
Repeat
Automatic repetition of manually set chloridation procedure
Dimensions, H x W x D
15 x 6 x 12 cm (5.9 x 2.4 x 4.7 in)
Weight
750 g (1.7 lb)
Catalog No.
$
The kit is suitable for producing intracellular microelectrodes using borosilicate glass capillaries with an integral
filament, see pages M45 to M49. The filament makes microelectrode filling easy. Special filling devices, such as vacuum
filling equipment, are therefore not required. In addition to
a transport and storage container for completed microelectrodes, the kit also includes a specially developed electrolytic chloridation unit for producing an AgCl film on silver electrodes, see to the left. Controlled application of the AgCl film
through the selection of current density and chloridation
period in the case of the electrolytic chloridation method,
together with the good reproducibility of the AgCl film, readily justify the increased cost of purchasing the electrical
chloridation equipment. By contrast, chemical chloridation
involves less equipment but produces poorer chloridation
results and has negative effects on the environment.
The kit includes:
Materials: Pack of 1.5 mm OD glass capillaries with filament, 10 m pure silver wire, 0.2 mm dia., fine
solder wire, emery paper, Scotch-Brite cleaning sponge, KCl and NaCl powder, connecting
pins for the microelectrodes, and sealing wax.
Tools:
Product
BS4 73-0004
1,316.00 HSE Auto Chloridizer Model 863, 115 VAC, 60 Hz
BS4 73-0005
1,3165.00 HSE Auto Chloridizer Model 863, 230 VAC, 50 Hz
BS4 73-0006
15.00 Spare Crocodile Clips
BS4 73-0007
18.00 Maintenance Kit for Silver Electrodes, Emery Paper and ScotchBrite Cleaning Sponge, for Cleaning Silver Electrode before
Chloridation
Cell Biology
For routine production of several identical electrodes, such as Ag/AgCl wires for glass
microelectrodes, the unit offers a simple programming feature for the chloridation
period in the repeat mode. First the learning mode is initiated by pressing the repeat
key (for longer than 1 second) and then an electrode is produced manually using
the start/stop key. All further electrodes are then prepared automatically according
to the manual setting by pressing the repeat key. The end of the chloridation process
is indicated by a beep and the current source is automatically switched off.
This starter kit makes entry into microelectrode production
technology easy for researchers who have not yet produced
any microelectrodes themselves. It contains all the necessary aids, components and consumables. A puller must be
purchased separately, see page M52. In addition to manual
dexterity which is always necessary, the user requires a
binocular transmitted-light microscope with eyepiece
graticule and up to 500X magnification for optical checking
of the tip diameter.
18 W, 230 V miniature, curved soldering
tweezers, straight soldering tweezers, fixation
tweezers, clamping stand as aid for soldering
the micro pins, electrolytic chloridation Auto
Chloridizer, transport and storage container
for micro-electrodes, special microelectrode
holder with miniature socket, and illustrated
book about the microelectrode technology.
Catalog No.
$
Product
BS4 73-0008
2,294.00 Microelectrode Starter Kit, 115 VAC,
60 Hz
BS4 73-0009
2,294.00 Microelectrode Starter Kit, 230 VAC,
50 Hz
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M29
Reference Electrodes
Specialized Tools For Bioresearch
Reference Cells & Ag-AgCI Electrodes
Why Silver-Silver Chloride (Ag-AgCl)?
Ag-AgCl electrodes have long been recognized for their superiority in sensing bioelectric signals. The electrochemical properties of Ag-AgCl
are such that biopotential waveforms are faithfully reproduced. The main feature that makes them superior to other metals is their low
offset potential. In a biomedical situation, the offset potential may be unstable or unpredictable. Long-term changes in offset potential
appear as baseline drift, and short-term changes as noise on the trace.
Thus, it is desirable that this potential be as low as possible. The electrode that shows minimum offset is the Ag-AgCl electrode. As the silver
chloride is ionized, chloride ions from the electrode are exchanged for similar ions in the tissue or electrolyte. The silver chloride is relatively stable regardless of the direction of the polarizing current flow.
To a great extent, the performance of Ag-AgCl electrodes depends on their formulation. Our electrodes are made from very high purity
materials. A careful proprietary process results in a fine grain, homogeneous mixture of silver and silver chloride. A careful sintering
process strengthens and stabilizes the entire electrode assembly.
Typical values of the key parameters, measured in 0.9% saline, between pairs of electrodes the same size are:
1) DC Offset Voltage: 180 µVolts
2) Drift: 25 µVolts/hour
3) Noise: 1 µVolt peak-to-peak (0.1-1000 Hz with 50/60 Hz eliminated).
E203
E200
E201
E210
E242
E204
E243
E244
E202
Ag-AgCl Pellet and Disc Electrodes
Disc Electrodes With Lead Wires
These six electrodes are ideally suited for making custom assemblies of
skin electrodes and other bioelectric recording and stimulation apparatus.
All have a pure silver wire (.25 mm diameter x 7 mm) embedded in the
Ag-AgCl matrix. This wire can be easily soldered or twisted to other wires.
These disc-type electrodes are made with a flexible 20 cm lead wire
attached to the back. The connection is encapsulated so that only the
surface and edges of the electrode can make contact. These electrodes can be used as direct-contact skin electrodes, or subcutaneously in temporary applications on research animals.
Catalog No.
$
BS4 64-1304
11.00 E200
Model
Pellet 1.5 x 3 mm (D x Thick)
Product
Catalog No.
$
BS4 64-1305
13.00 E201
Pellet 2.0 x 4 mm (D x Thick)
BS4 64-1314
18.00 E242
Disc Electrode 4.0 mm D
BS4 64-1313
10.00 E210
Pellet 2.0 x 2 mm (D x Thick)
BS4 64-1315
21.00 E243
Disc Electrode 8.0 mm D
BS4 64-1316
27.00 E244
Disc Electrode 12.5 mm D
BS4 64-1306
13.00 E202
Disc 4.0 x 1 mm (D x Thick)
BS4 64-1307
16.00 E203
Disc 8.0 x 1 mm (D x Thick)
BS4 64-1308
20.00 E204
Disc 12.5 x 1 mm (D x Thick)
Ag-AgCl Pellet and Wire
Electrodes
Model
Product
Flat Tip Probes
Ag-AgCl cylinders, encapsulated
in sturdy, insulating epoxy
tubes; these probes are ideal for
use in ionic solutions. They can
be resurfaced frequently with
fine sandpaper. Terminated with
a 2 mm tinned copper wire for
connection to your leads.
Pellet electrodes have cylindrical pellet embedded on .25 x 70 mm (D x L) wire.
Wire electrodes have a uniform coating of AgAgCl over the last 10 mm of a 0.25 x 70 mm (D
x L) wire. Maximum diameter of the Ag-AgCl
matrix is 0.8 mm.
aE207
E208
Note: All electrodes suitable for probing tissues.
M30
Cat. No.
$
BS4 64-1309
16.00 E205
Model
Product
Pellet Electrode
1.0 mm D
BS4 64-1310
14.00 E206
Pellet Electrode
2.0 mm D
BS4 64-1317
19.00 E255
Wire Electrode
0.8 mm D
E205 E206 E255
Catalog No.
$
BS4 64-1311
26.00 E207
Model
Sensor Tip 2 x 4 mm (D x Thick)
Epoxy Tube 3.5 x 25 mm (D x L)
Product
BS4 64-1312
22.00 E208
Sensor Tip 2 x 4 mm (D x Thick)
Epoxy Tube 5 x 50 mm (D x L)
11,000 Specialty Products to Enhance Your Bioresearch
Reference Electrodes
Reference Cells & Ag-AgCI Electrodes
WA30
WC1
REF-1L
WA10
REF-2L
REF-3L
WC2
PJ1
PJ2
TJ1
Reference Cells
Ag-AgCl electrodes are epoxy encapsulated in a plastic shell. They
can be used as preparation reference or ground electrodes. Around
4.5 mm D, they are available with 2 mm pin, 2 mm jack and wire
connection. All have a Luer taper for convenient mounting in chambers and other devices.
Plugs and Jacks
Catalog No.
$
Catalog No.
$
Product
BS4 64-1325
11.00 WC1-10
1 mm Pin, pkg. of 10
BS4 64-1301
30.00 REF-1L
Reference Cell with 2 m L Wire
BS4 64-1326
14.00 WC2-5
2 mm Pin, pkg. of 5
BS4 64-1302
30.00 REF-2L
Reference Cell with 2 mm Pin
BS4 64-1322
27.00 PJ1-10
1 mm Jack, Uninsulated, pkg. of 10
Reference Cell with 2 mm Jack
BS4 64-1323
14.00 PJ2-5
2 mm Jack, Uninsulated, pkg. of 5
BS4 64-1324
15.00 TJ1-3
1 mm Jack, Teflon® Insulated,
pkg. of 3
BS4 64-1328
15.00 WA30-5
1 mm Pin with 30 cm L, 26 ga.
Insulated Wire, pkg. of 3
BS4 64-1327
18.00 WA10-5
1 mm Pin with 10 cm L, .25 mm D
Bare Silver Wire, pkg. of 2
BS4 64-1303
Model
30.00 REF-3L
Silver Wire
Made of annealed silver 99.99% pure. Supplied for the convenience
of researchers to fashion their own electrodes.
$
BS4 64-1318
12.00 AG8W
Model
Silver Wire 0.20 mm (.008") D, 2 m
L, No Insulation
BS4 64-1319
18.00 AG10W
Silver Wire 0.25 mm (.010") D, 2 m
L, No Insulation
BS4 64-1320
21.00 AG15W
Silver Wire 0.37 mm (.015") D, 2 m
L, No Insulation
BS4 64-1321
30.00 AGT10W
Silver Wire 0.25 mm (.010") D, 2 m
L, Teflon® Insulation
Model
Product
Product
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
Cell Biology
Catalog No.
An assortment of gold-plated 1 mm plugs and jacks, with or without
wires. Useful for grounding, shielding, etc.
M31
Electrode Holders
E Series Holders
Applications
ESW-MxxP
E45-FxxN
•
Intracellular Recording
•
Microiontophoresis
Specifications
ESP-FxxN
Specialized Tools For Bioresearch
ERW-FxxN
Acrylic
Body Styles
Straight, 45°, and 90°
Connectors
2 mm Pin or 2 mm Jack
Coupling
Ag Wire or
Ag-AgCl Pellet
Port
2 mm diameter Polycarbonate
Vent
0.8 mm diameter Hole
Standard Glass Sizes 1.0, 1.2, 1.5 and 2.0 mm
E45-MxxP
ERP-MxxP
E SERIES-Straight Body Holders
Catalog No.
$
Wire/Pellet Connector
Port
BS4 64-0992
40.00
Pellet
No
2 mm Pin
BS4 64-0993
BS4 64-0994
40.00
BS4 64-1260
BS4 64-0995
41.00
Yes
BS4 64-0996
BS4 64-0997
41.00
BS4 64-0998
BS4 64-0980
40.00
2 mm Jack
No
BS4 64-0981
BS4 64-0982
40.00
BS4 64-0983
BS4 64-0984
41.00
Yes
BS4 64-0985
BS4 64-0986
41.00
BS4 64-0987
BS4 64-1011
40.00
Wire
2 mm Pin
No
BS4 64-1012
BS4 64-1013
40.00
BS4 64-1014
BS4 64-1015
41.00
BS4 64-1016
BS4 64-1017
BS4 64-1018
M32
Body Material
41.00
Yes
Glass
OD (mm)
Model
1.0
ESP-M10N
1.2
ESP-M12N
1.5
ESP-M15N
2.0
ESP-M20N
1.0
ESP-M10P
1.2
ESP-M12P
1.5
ESP-M15P
2.0
ESP-M20P
1.0
ESP-F10N
1.2
ESP-F12N
1.5
ESP-F15N
2.0
ESP-F20N
1.0
ESP-F10P
1.2
ESP-F12P
1.5
ESP-F15P
2.0
ESP-F20P
1.0
ESW-M10N
1.2
ESW-M12N
1.5
ESW-M15N
2.0
ESW-M20N
1.0
ESW-M10P
1.2
ESW-M12P
1.5
ESW-M15P
2.0
ESW-M20P
11,000 Specialty Products to Enhance Your Bioresearch
port
port
port
Electrode Holders
E Series Holders
E SERIES-Straight Body Holders (Continued)
Catalog No.
$
Wire/Pellet Connector
Port
Glass
OD (mm)
Model
BS4 64-0999
40.00
Wire
No
1.0
ESW-F10N
1.2
ESW-F12N
1.5
ESW-F15N
2.0
ESW-F20N
1.0
ESW-F10P
1.2
ESW-F12P
1.5
ESW-F15P
2.0
ESW-F20P
1.0
ESW-F10V
1.2
ESW-F12V
1.5
ESW-F15V
2.0
ESW-F20V
Model
2 mm Jack
BS4 64-1000
BS4 64-1001
40.00
BS4 64-1002
BS4 64-1003
41.00
Yes
BS4 64-1004
BS4 64-1005
41.00
BS4 64-1006
BS4 64-1007
41.00
Vent*
BS4 64-1008
BS4 64-1009
41.00
BS4 64-1010
port
*Vented models are standard with the Warner OC-725 Oocte Clamp.
E Series-45º Body Holders
$
Wire/Pellet Connector
Port
Glass
OD (mm)
BS4 64-0908
42.00
Pellet
No
1.0
E45P-M10N
1.2
E45P-M12N
1.5
E45P-M15N
2.0
E45P-M20N
2 mm Pin
BS4 64-0909
BS4 64-0910
42.00
BS4 64-0911
BS4 64-0912
45.00
Yes
BS4 64-0913
BS4 64-0914
45.00
BS4 64-0915
BS4 64-0900
42.00
2 mm Jack
No
BS4 64-0901
BS4 64-0902
42.00
BS4 64-0903
BS4 64-0904
45.00
Yes
BS4 64-0905
BS4 64-0906
45.00
BS4 64-0907
BS4 64-0924
42.00
Wire
2 mm Pin
No
BS4 64-0925
BS4 64-0926
42.00
BS4 64-0927
BS4 64-0928
44.00
Yes
BS4 64-0929
BS4 64-0930
44.00
BS4 64-0931
BS4 64-0916
42.00
BS4 64-0917
BS4 64-0918
BS4 64-0919
42.00
Wire
2 mm Jack
No
1.0
E45P-M10P
1.2
E45P-M12P
1.5
E45P-M15P
2.0
E45P-M20P
1.0
E45P-F10N
1.2
E45P-F12N
1.5
E45P-F15N
2.0
E45P-F20N
1.0
E45P-F10P
1.2
E45P-F12P
1.5
E45P-F15P
2.0
E45P-F20P
1.0
E45W-M10N
1.2
E45W-M12N
1.5
E45W-M15N
2.0
E45W-M20N
1.0
E45W-M10P
1.2
E45W-M12P
1.5
E45W-M15P
2.0
E45W-M20P
1.0
E45W-F10N
1.2
E45W-F12N
1.5
E45W-F15N
2.0
E45W-F20N
Cell Biology
Catalog No.
port
port
port
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M33
Electrode Holders
E Series Holders
E SERIES-45º Body Holders (Continued)
Catalog No.
$
BS4 64-0920
44.00
Wire/Pellet Connector
Port
Glass
OD (mm)
Model
Yes
1.0
E45W-F10P
1.2
E45W-F12P
1.5
E45W-F15P
2.0
E45W-F20P
BS4 64-0921
BS4 64-0922
44.00
BS4 64-0923
port
*Vented models are standard with the Warner OC-725 Oocte Clamp.
Specialized Tools For Bioresearch
E SERIES-90º Body Holders
Catalog No.
$
Wire/Pellet Connector
Port
Glass
OD (mm)
Model
BS4 64-0940
42.00
Pellet
No
1.0
ERP-M10N
2 mm Pin
BS4 64-0941
BS4 64-0942
42.00
BS4 64-0943
BS4 64-0944
44.00
Yes
BS4 64-0945
BS4 64-0946
44.00
BS4 64-0947
BS4 64-0932
42.00
2 mm Jack
No
BS4 64-0933
BS4 64-0934
42.00
BS4 64-0935
BS4 64-0936
44.00
Yes
BS4 64-0937
BS4 64-0938
44.00
BS4 64-0939
BS4 64-0956
42.00
Wire
2 mm Pin
No
BS4 64-0957
BS4 64-0958
42.00
BS4 64-0959
BS4 64-0960
44.00
Yes
BS4 64-0961
BS4 64-0962
44.00
BS4 64-0963
BS4 64-0948
42.00
2 mm Jack
No
BS4 64-0949
BS4 64-0950
42.00
BS4 64-0951
BS4 64-0952
44.00
Yes
BS4 64-0953
BS4 64-0954
44.00
BS4 64-0955
1.2
ERP-M12N
1.5
ERP-M15N
2.0
ERP-M20N
1.0
ERP-M10P
1.2
ERP-M12P
1.5
ERP-M15P
2.0
ERP-M20P
1.0
ERP-F10N
1.2
ERP-F12N
1.5
ERP-F15N
2.0
ERP-F20N
1.0
ERP-F10P
1.2
ERP-F12P
1.5
ERP-F15P
2.0
ERP-F20P
1.0
ERW-M10N
1.2
ERW-M12N
1.5
ERW-M15N
2.0
ERW-M20N
1.0
ERW-M10P
1.2
ERW-M12P
1.5
ERW-M15P
2.0
ERW-M20P
1.0
ERW-F10N
1.2
ERW-F12N
1.5
ERW-F15N
2.0
ERW-F20N
1.0
ERW-F10P
1.2
ERW-F12P
1.5
ERW-F15P
2.0
ERW-F20P
*Vented models are standard with the Warner OC-725 Oocte Clamp.
M34
11,000 Specialty Products to Enhance Your Bioresearch
port
port
port
port
Electrode Holders
E Series Holders with Handle
Holders include a 6.3 mm diameter X 6.3 cm long handle for mounting in a micropositioner. Handles are screwed together except for
vented models which are joined by a pin and jack for easy removal
from the handle.
E SERIES with Handle
Catalog No.
$
Wire/Pellet Connector
Port
BS4 64-1023
51.00
Pellet
No
2 mm Jack
BS4 64-1024
BS4 64-1025
51.00
BS4 64-1026
BS4 64-1039
52.00
Yes
BS4 64-1040
BS4 64-1041
52.00
BS4 64-1042
BS4 64-1019
51.00
Wire
2 mm Jack
No
BS4 64-1020
BS4 64-1021
51.00
BS4 64-1022
BS4 64-1035
52.00
Yes
BS4 64-1036
BS4 64-1037
52.00
BS4 64-1051
52.00
BS4 64-1052
BS4 64-1053
52.00
BS4 64-1054
Vent*
Model
1.0
E45P-F10NH
1.2
E45P-F12NH
1.5
E45P-F15NH
2.0
E45P-F20NH
1.0
E45P-F10PH
1.2
E45P-F12PH
1.5
E45P-F15PH
2.0
E45P-F20PH
1.0
E45W-F10NH
1.2
E45W-F12NH
1.5
E45W-F15NH
2.0
E45W-F20NH
1.0
E45W-F10PH
1.2
E45W-F12PH
1.5
E45W-F15PH
2.0
E45W-F20PH
1.0
E45W-F10VH
1.2
E45W-F12VH
1.5
E45W-F15VH
2.0
E45W-F20VH
port
port
*Vented models are standard with the Warner OC-725 Oocte Clamp.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
Cell Biology
BS4 64-1038
Glass
OD (mm)
M35
Electrode Holders
Q Series Holders
Applications
QSW-Bxxp
•
Patch Clamp Recording
•
Intracellular Recording
QSW-TxxP
Specifications
Q45W-AxxP
Specialized Tools For Bioresearch
QRW-AxxN
Compatible with Warner Patch Clamps PC-501 & PC-505 and Axon Patch Clamps
(older style prior to 2/1/95)
Polycarbonate
Body Styles
Straight, 45°, and 90°
Connectors
1 mm Pin, 1 mm Pin Threaded
and BNC
Coupling
Ag Wire or Ag-AgCl Hybrid
Port
2 mm diameter Polycarbonate
Standard Glass Sizes 1.0, 1.2, 1.5, 1.7 and 2.0 mm
NOTE: Holders without ports are not suitable for patch clamp recording.
Q SERIES Holders with 1 mm Pin
Catalog No.
$
Wire/Pellet Connector
Port
BS4 64-1105
54.00
Wire
No
Straight
BS4 64-1107
QSW-A10N
QSW-A12N
54.00
1.5
QSW-A15N
1.7
QSW-A17N
54.00
2.0
QSW-A20N
BS4 64-0821
BS4 64-0822
Yes
1.0
QSW-A10P
1.2
QSW-A12P
1.5
QSW-A15P
1.7
QSW-A17P
2.0
QSW-A20P
1.0
Q45W-A10N
1.2
Q45W-A12N
63.00
1.5
Q45W-A15N
1.7
Q45W-A17N
63.00
2.0
Q45W-A20N
58.00
BS4 64-0823
BS4 64-0978
58.00
BS4 64-0824
BS4 64-0841
63.00
45°
No
BS4 64-0842
BS4 64-0843
BS4 64-0844
BS4 64-1055
BS4 64-1056
Yes
1.0
Q45W-A10P
1.2
Q45W-A12P
1.5
Q45W-A15P
1.7
Q45W-A17P
2.0
Q45W-A20P
1.0
QRW-A10N
1.2
QRW-A12N
63.00
1.5
QRW-A15N
1.7
QRW-A17N
63.00
2.0
QRW-A20N
69.00
BS4 64-1057
BS4 64-0968
69.00
BS4 64-1058
BS4 64-0861
63.00
BS4 64-0862
BS4 64-0863
BS4 64-1103
BS4 64-0864
Model
1.0
BS4 64-0745
BS4 64-1108
Glass
OD (mm)
1.2
BS4 64-1106
BS4 64-1098
M36
Body Material
90°
No
11,000 Specialty Products to Enhance Your Bioresearch
port
port
Electrode Holders
Q Series Holders
Q SERIES Holders with 1 mm Pin (Continued)
Catalog No.
$
BS4 64-1075
69.00
Wire/Pellet Connector
Port
Glass
OD (mm)
Model
Yes
1.0
QRW-A10P
1.2
QRW-A12P
1.5
QRW-A15P
1.7
QRW-A17P
2.0
QRW-A20P
1.5
QSH-A15N
1.7
QSH-A17N
2.0
QSH-A20N
1.5
QSH-A15P
1.7
QSH-A17P
2.0
QSH-A20P
BS4 64-1076
BS4 64-1077
69.00
BS4 64-0973
BS4 64-1078
69.00
BS4 64-1351
BS4 64-1352
Hybrid
Straight
No
81.00
BS4 64-1353
BS4 64-1354
84.00
Yes
BS4 64-1355
BS4 64-1356
84.00
port
port
Compatible with: Axon Patch Clamps and Microelectrode Amps post 2/1/95
NOTE: Holders without ports are not suitable for patch clamp recording.
Q SERIES Holders with 1 mm Pin, Threaded Collar
Catalog No.
$
Wire/Pellet Connector
Port
Glass
OD (mm)
Model
BS4 64-1121
75.00
Wire
No
1.0
QSW-T10N
Straight
BS4 64-1122
BS4 64-1123
QSW-T12N
1.5
QSW-T15N
1.7
QSW-T17N
75.00
2.0
QSW-T20N
1.0
QSW-T10P
1.2
QSW-T12P
1.5
QSW-T15P
1.7
QSW-T17P
2.0
QSW-T20P
1.0
Q45W-T10N
BS4 64-0749
BS4 64-1124
BS4 64-0837
BS4 64-0838
Yes
79.00
BS4 64-0839
BS4 64-1097
79.00
BS4 64-0840
BS4 64-0857
80.00
45°
No
BS4 64-0858
BS4 64-0859
1.2
Q45W-T12N
80.00
1.5
Q45W-T15N
1.7
Q45W-T17N
80.00
2.0
Q45W-T20N
1.0
Q45W-T10P
1.2
Q45W-T12P
1.5
Q45W-T15P
1.7
Q45W-T17P
2.0
Q45W-T20P
1.0
QRW-T10N
BS4 64-1102
BS4 64-0860
BS4 64-1071
BS4 64-1072
Yes
84.00
BS4 64-1073
BS4 64-0972
84.00
BS4 64-1074
BS4 64-0877
80.00
BS4 64-0878
BS4 64-0879
No
1.2
QRW-T12N
80.00
1.5
QRW-T15N
1.7
QRW-T17N
80.00
2.0
QRW-T20N
BS4 64-0744
BS4 64-0880
90°
Cell Biology
1.2
75.00
port
port
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M37
Electrode Holders
Q Series Holders
Q SERIES Holders with 1 mm Pin, Threaded Collar (Continued)
Catalog No.
$
BS4 64-1091
84.00
Wire/Pellet Connector
Port
Glass
OD (mm)
Model
Yes
1.0
QRW-T10P
1.2
QRW-T12P
1.5
QRW-T15P
1.7
QRW-T17P
2.0
QRW-T20P
1.5
QSH-T15N
1.7
QSH-T17N
2.0
QSH-T20N
1.5
QSH-T15P
1.7
QSH-T17P
2.0
QSH-T20P
BS4 64-1092
BS4 64-1093
84.00
BS4 64-0977
BS4 64-1094
84.00
BS4 64-1363
BS4 64-1364
Hybrid
Straight
No
102.00
BS4 64-1365
BS4 64-1366
105.00
Yes
Specialized Tools For Bioresearch
BS4 64-1367
BS4 64-1368
105.00
port
Compatible with: Heka, List and Dagan Patch Clamps
NOTE: Holders without ports are not suitable for patch clamp recording.
Q SERIES Holders with BNC Connector
Catalog No.
$
Wire/Pellet Connector
Port
Glass
OD (mm)
Model
BS4 64-1109
54.00
Wire
No
1.0
QSW-B10N
Straight
BS4 64-1110
BS4 64-1111
1.2
QSW-B12N
QSW-B15N
1.7
QSW-B17N
2.0
QSW-B20N
1.0
QSW-B10P
58.00
1.2
QSW-B12P
1.5
QSW-B15P
58.00
1.7
QSW-B17P
2.0
QSW-B20P
1.0
Q45W-B10N
BS4 64-0746
BS4 64-1112
54.00
BS4 64-0825
BS4 64-0826
BS4 64-0979
Yes
BS4 64-0828
BS4 64-0845
63.00
45°
No
BS4 64-0846
BS4 64-0847
1.2
Q45W-B12N
1.5
Q45W-B15N
1.7
Q45W-B17N
2.0
Q45W-B20N
1.0
Q45W-B10P
69.00
1.2
Q45W-B12P
1.5
Q45W-B15P
69.00
1.7
Q45W-B17P
2.0
Q45W-B20P
63.00
BS4 64-1099
BS4 64-0848
63.00
BS4 64-1059
BS4 64-1060
Yes
BS4 64-1061
BS4 64-0969
BS4 64-1062
Q45-BxxP
1.5
54.00
BS4 64-0827
M38
port
11,000 Specialty Products to Enhance Your Bioresearch
port
port
Electrode Holders
Q Series Holders
Q SERIES Holders with BNC Connector (Continued)
Catalog No.
$
BS4 64-0865
63.00
Wire/Pellet Connector
90°
Port
Glass
OD (mm)
Model
No
1.0
QRW-B10N
1.2
QRW-B12N
1.5
QRW-B15N
1.7
QRW-B17N
2.0
QRW-B20N
1.0
QRW-B10P
1.2
QRW-B12P
1.5
QRW-B15P
1.7
QRW-B17P
2.0
QRW-B20P
1.5
QSH-B15N
1.7
QSH-B17N
2.0
QSH-B20N
1.5
QSH-B15P
1.7
QSH-B17P
2.0
QSH-B20P
BS4 64-0866
BS4 64-0867
63.00
BS4 64-1104
BS4 64-0868
63.00
BS4 64-1079
BS4 64-1080
Yes
69.00
BS4 64-1081
BS4 64-0974
69.00
BS4 64-1082
BS4 64-1357
81.00
Hybrid
Straight
No
BS4 64-1358
BS4 64-1359
81.00
BS4 64-1360
BS4 64-1361
BS4 64-1362
Yes
84.00
port
port
Cell Biology
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M39
Electrode Holders
PE Series Holders
Applications
•
Microperfusion (perfusion at the pipette tip)*
Specialized Tools For Bioresearch
Specifications
PESW-BxxP
Body Material
Polycarbonate
Body Style
Straight
Connectors
1 mm Pin, 1 mm Pin Threaded and BNC
Coupling
Ag Wire
Port
2 mm diameter Polycarbonate
Perfusion Port
30° port accepts tubing up to 1 mm diameter
Standard Glass Sizes
1.0, 1.2, 1.5, 1.7 and 2.0 mm
PESW-TxxP
Patch Clamp Compatibility
Connector
For Use With
1 mm Pin
Warner Patch Clamp Models PC-501
& PC-505 Axon Patch Clamps prior
to 2/1/95
BNC
List, Heka and Dagan Patch Clamps
1 mm Pin with
threaded collar
Axon Patch Clamps and Microelectrode
Amps prior to 2/1/95
For Microperfusion
(perfusing inside the micropipette)
Perfusion at the electrode tip is possible with the PE Series holders. They include
an additional port at 30° to the glass bore allowing for the insertion of microbore tubing into the pipette. This port includes a threaded cap and seal for up to
1 mm diameter tubing. See the reports referenced below for information on the
microperfusion technique.
PE SERIES Holders
Catalog No.
$
Wire/Pellet Body Style
Port
Connector
BS4 64-1144
84.00
Wire
Yes
1 mm Pin
Straight
BS4 64-1145
BS4 64-1146
84.00
BS4 64-1371
BS4 64-1147
84.00
BS4 64-1148
BS4 64-1149
BNC
84.00
BS4 64-1150
BS4 64-1369
84.00
BS4 64-1151
BS4 64-1160
PESW-A10P
1.2
PESW-A12P
1.5
PESW-A15P
1.7
PESW-A17P
2.0
PESW-A20P
1.0
PESW-B10P
1.2
PESW-B12P
1.5
PESW-B15P
1.7
PESW-B17P
2.0
PESW-B20P
1.0
PESW-T10P
1 mm pin
Threaded
1.2
PESW-T12P
109.00
Collar
1.5
PESW-T15P
1.7
PESW-T17P
2.0
PESW-T20P
BS4 64-1373
BS4 64-1163
1.0
109.00
BS4 64-1161
BS4 64-1162
Glass OD
(mm)
Model
109.00
*References:
M40
1)
Nehr, E. and Eckert, R.: Fast patch-pipette internal perfusion with minimum solution
flow. Grinnel, A.D., Armstrong, D. and Jackson, M.B.: Calcium and Ion Channel
Modulation, Plenum Press, N.Y.
2)
Cull-Candy, S.G., Miledi, R. and Parker, I.: Single glutamate-activated channels recorded
from locust muscle fibers with perfused patch clamp electrodes. J. Physiology 32-, 195210.
3)
Pusch, M. and Nehr, E. (1987) Kinetics of loading small cells with various compounds
by use of patch pipettes. Pflugers Archives (Spring Meeting of the Physiol. Ges.).
4)
Tang, J.M., Wang, J. and Eisenberg, R.S. (1992) Perfusing patch pipettes. Methods in
Enzymol. 207, 176-181.
11,000 Specialty Products to Enhance Your Bioresearch
port
port
port
Electrode Holders
ME Series Holders
Specifications
Applications
•
Intracellular and
Extracellular Recording
•
Microiontophoresis
Pressure Ports
A – Accepts 1.5 mm (1/16") tubing
B – Accepts 2.4 mm (3/32") tubing
Body Material
9.3 mm diameter
acrylic
Body Style
Straight
Connector
2 mm jack
Coupling
Ag/AgCl pellet or
Ag Wire
MEW-FxxT
Standard Glass Sizes 1.0, 1.2, 1.5 and
2.0 mm
Handle
ML – Accepts female Luer fitting
6.3 mm diameter
x 6.3 cm long,
threaded to
attach to holder.
MEW-FxxFL
FL – Accepts male Luer fitting
T* – Accepts 1/4-28 male thread
fitting
* Holders with the T port are compatible with the Picospritzer® system made by General Valve Corp.
PE SERIES Holders
Catalog No.
$
Wire/Pellet Body Style
Port
Connector
BS4 64-1220
57.00
Straight
Wire
1/16" barb
2 mm Jack
BS4 64-1221
BS4 64-1222
57.00
BS4 64-1223
BS4 64-1224
57.00
1/32" barb
BS4 64-1225
BS4 64-1226
57.00
BS4 64-1232
57.00
male Luer
BS4 64-1233
BS4 64-1234
57.00
BS4 64-1235
BS4 64-1228
57.00
female Luer
BS4 64-1229
BS4 64-1230
57.00
BS4 64-1231
BS4 64-1236
62.00
BS4 64-1237
BS4 64-1238
Straight
2 mm Jack
Pellet
BS4 64-1192
BS4 64-1194
BS4 64-1195
57.00
MEW-F12B
1.5
MEW-F15B
2.0
MEW-F20B
1.0
MEW-F10ML
1.2
MEW-F12ML
1.5
MEW-F15ML
2.0
MEW-F20ML
1.0
MEW-F10FL
1.2
MEW-F12FL
1.5
MEW-F15FL
2.0
MEW-F20FL
MEW-F15T
2.0
MEW-F20T
1/16" barb
1/32" barb
57.00
57.00
1.2
1.5
BS4 64-1187
BS4 64-1193
MEW-F10B
MEW-F10T
BS4 64-1185
BS4 64-1186
MEW-F20A
1.0
MEW-F12T
57.00
57.00
2.0
1.2
BS4 64-1183
BS4 64-1184
MEW-F15A
1/4-28
BS4 64-1181
BS4 64-1182
1.5
1.0
62.00
57.00
MEW-F10A
MEW-F12A
threaded
BS4 64-1239
BS4 64-1180
1.0
1.2
male Luer
1.0
MEP-F10A
1.2
MEP-F12A
1.5
MEP-F15A
2.0
MEP-F20A
1.0
MEP-F10B
1.2
MEP-F12B
1.5
MEP-F15B
2.0
MEP-F20B
1.0
MEP-F10ML
1.2
MEP-F12ML
1.5
MEP-F15ML
2.0
MEP-F20ML
port (A)
port (B)
port (ML)
port (FL)
Cell Biology
BS4 64-1227
Glass OD
(mm)
Model
port (T)
port (A)
port (B)
port (ML)
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M41
Electrode Holders
ME Series Holders
PE SERIES Holders (Continued)
Catalog No.
$
BS4 64-1188
57.00
Wire/Pellet Body Style
Port
Connector
Glass OD
(mm)
Model
female Luer
BS4 64-1189
BS4 64-1190
57.00
BS4 64-1191
BS4 64-1196
56.00
threaded
BS4 64-1197
BS4 64-1198
1/4-28
56.00
Specialized Tools For Bioresearch
BS4 64-1199
MEP-F10FL
MEP-F12FL
1.5
MEP-F15FL
2.0
MEP-F20FL
1.0
MEP-F10T
1.2
MEP-F12T
1.5
MEP-F15T
2.0
MEP-F20T
port (FL)
port (T)
MP Series Holders
Specifications
Applications
•
Microinjection
Body Material
Acrylic
Body Style
Straight
Port
1
⁄16" barbed (1⁄16" tubing
ID), 3⁄32" barbed (3⁄32"
tubing ID), male Luer,
female Luer and 1⁄4-28
female threaded
Pressure Ports
A – Accepts 1.5 mm (1/16") tubing
B – Accepts 2.4 mm (3/32") tubing
ML – Accepts female Luer fitting
FL – Accepts male Luer fitting
Standard
Glass Sizes
Handle
T* – Accepts 1/4-28 male thread
fitting
* Holders with the T port are compatible
MPthe
SERIES
Holders
®
with
Picospritzer
system made by
General Valve Corp.
Catalog No.
$
Body Style
Port
BS4 64-1261
36.00
Straight
1/16" barb
BS4 64-1262
BS4 64-1263
36.00
BS4 64-1264
BS4 64-1265
36.00
1/32" barb
BS4 64-1266
BS4 64-1267
36.00
BS4 64-1268
BS4 64-1273
36.00
male Luer
BS4 64-1274
BS4 64-1275
36.00
BS4 64-1276
BS4 64-1269
36.00
female Luer
BS4 64-1270
BS4 64-1271
36.00
BS4 64-1272
BS4 64-1278
40.00
BS4 64-1279
BS4 64-1280
BS4 64-1281
M42
1.0
1.2
40.00
MP-SxxB
1.0, 1.2, 1.5 and
2.0 mm
6.3 mm diameter x
6.3 cm long, threaded
to attach to holder.
Glass OD
(mm)
MP-SxxT
Model
1.0
MP-S10A
1.2
MP-S12A
1.5
MP-S15A
2.0
MP-S20A
1.0
MP-S10B
1.2
MP-S12B
1.5
MP-S15B
2.0
MP-S20B
1.0
MP-S10ML
1.2
MP-S12ML
1.5
MP-S15ML
2.0
MP-S20ML
1.0
MP-S10FL
1.2
MP-S12FL
1.5
MP-S15FL
2.0
MP-S20FL
threaded
1.0
MP-S10T
1/4-28
1.2
MP-S12T
1.5
MP-S15T
2.0
MP-S20T
port (A)
port (B)
port (ML)
port (FL)
port (T)
11,000 Specialty Products to Enhance Your Bioresearch
Electrode Holders
Theta Glass Holders
Specifications
Applications
•
Microinjection or
microiontophoresis with
theta glass
Body Material
Acrylic
Body Style
Straight
Coupling
Ag wire (2)
Connector
2 mm jack
Port
2 mm diameter polycarbonate
Standard Glass Sizes
1.5 and 2.0 mm
Handle
6.3 mm diameter x 6.3 cm
long, threaded to attach to
holder.
* Holders with the T port are compatible
Theta
Holders
®
with
the Glass
Picospritzer
system made by
General Valve Corp.
Catalog No.
$
Port
Handle
BS4 64-1164
77.00
No
No
BS4 64-1165
BS4 64-1172
95.00
Yes
BS4 64-1173
BS4 64-1168
88.00
Yes
No
BS4 64-1169
BS4 64-1176
105.00
Yes
BS4 64-1177
Glass OD
(mm)
THS-FxxP
Model
1.5
THS-F15
2.0
THS-F20
1.5
THS-F15H
2.0
THS-F20H
1.5
THS-F15P
2.0
THS-F20P
1.5
THS-F15PH
2.0
THS-F20PH
port
port
Electrode/Manifold Holders MHH-25, MHH-38
Cell Biology
Specifications
The MHH-25 and MHH-38 Holders permit
convenient mounting of manifolds and electrode holders or other devices with 6.3 or
9.5 mm diameters. The holder head can be
pivoted ±90° from the axial position and
rotated 360° about the axis. Friction holds
the head firmly in the set position. Holder
head and coupler are made from Delrin®.
The anodized aluminum handle is 6.3 mm
diameter x 6.3 cm long and will fit most
positioners.
MHH-38 and MHH-25
Device Compatibility
Catalog No.
BS4 64-0218
BS4 64-0219
$
Model
Description
65.00
MHH-25
E and Q Series Electrode Holders
MHH-38
PE Series and Theta Electrode Holders MP and MPP Series
Manifolds
A Diameter
Model
Diameter
MHH-25
6.3 mm (1/4”)
MHH-38
9.5 mm (3/8”)
A
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M43
Electrode Holders
Electrode/Manifold Holders MHH-25, MHH-38
Microelectrode Holder and Replacement Parts
Catalog No.
$
Qty/Pkg
Description
Coupling Elements
BS4 64-1297
BS4 64-1282
9.00
BS4 64-1288
3
SP-1M
Molded Ag-AgCl pellet assembly
10
AG25-10
Ag wire, 0.25 mm diameter x 70 mm long
1
HP-205
Hybrid Ag-AgCl pellet/Ag wire assembly
HP-205
SP-1M
AG-25
Pipette and Wire Seals
BS4 64-1289
BS4 64-1290
10
PS-10
Pipette seal for 1.0 mm diameter glass
10.00
10
PS-12
Pipette seal for 1.2 mm diameter glass
10
PS-15
Pipette seal for 1.5 mm diameter glass
10.00
10
PS-17
Pipette seal for 1.7 mm diameter glass
10
PS-20
Pipette seal for 2.0 mm diameter glass
10
WS-1
Wire seal for E, ME, MP and theta holder
10
WS-2
Wire seal for Q and PE Series holders
10
WS-3
Wire seal for Q and PE Series holders with
threaded “T” connector
2
QC-10
Compression cap for 1.0 mm glass holders
2
QC-12
Compression cap for 1.2 mm glass holders
2
QC-15
Compression cap for 1.5 mm glass holders
2
QC-17
Compression cap for 1.7 mm glass holders
2
QC-20
Compression cap for 2.0 mm glass holders
3
HC-10M
1 mm pin for Q and PE holders with A or T
connectors
3
HC-13M
1.3 mm pin for Q and PE holders with B
(BNC) connector
3
HC-20M
2 mm threaded pin for E, ME and theta holders
3
HC-21F
2 mm jack assembly for all series holders
3
HC-22M
2 mm pin for Q and PE holders with M connector
BS4 64-1291
BS4 64-1374
Specialized Tools For Bioresearch
BS4 64-1292
M44
BS4 64-1298
10.00
BS4 64-1299
BS4 64-1300
10.00
PS-xx
WS-1
WS-2
WS-3
Glass Seal Compression Caps
BS4 64-1293
15.00
BS4 64-1294
BS4 64-1295
15.00
BS4 64-1375
BS4 64-1296
15.00
Connector Pins and Jacks
BS4 64-1283
16.00
BS4 64-1284
BS4 64-1285
19.00
BS4 64-1286
BS4 64-1287
19.00
11,000 Specialty Products to Enhance Your Bioresearch
QC-xx
Capillary Glass
Premium Capillary Glass
Premium Thin Wall Borosilicate
Premium Thin Wall Borosilicate
•
Ends are fire polished to prevent damage to the rubber gaskets when inserted into electrode holders
•
Glass is also cleaned with deionized water before
being packed in dust-free containers
Catalog No.
$
Model
OD mm ID mm Length
Qty/Pkg
BS4 64-0777
31.00
G100T-3
1.0
0.78
75 mm
500
G100T-4
1.0
0.78
100 mm
500
G100T-6
1.0
0.78
150 mm
500
G120T-3
1.2
0.94
75 mm
350
G120T-4
1.2
0.94
100 mm
350
G120T-6
1.2
0.94
150 mm
350
G150T-3
1.5
1.17
75 mm
225
G150T-4
1.5
1.17
100 mm
225
G150T-6
1.5
1.17
150 mm
225
BS4 64-0778
BS4 64-0779
46.00
BS4 64-0780
Warner capillary glass is known worldwide for its consistent high
quality. The glass is precision drawn to insure reliability and consistency from batch to batch. The full line of glass capillaries listed
here is stocked for fast shipment.
BS4 64-0781
Standard Wall/Thin Wall
Tubing is available in two wall thickness, standard wall and thin
wall. Additionally, a variety of diameters is offered to cover most
needs of micropipette and microelectrode research.
BS4 64-0785
39.00
BS4 64-0782
BS4 64-0783
28.00
BS4 64-0784
46.00
Premium Standard Wall
Borosilicate with Filament
Premium Standard Wall Borosilicate
Premium Corning Type 7740 (Pyrex)
Corning 7740 Borosilicate is the
Specifications
most commonly used glass in
Composition
81% SiO2,
electrode fabrication because of
13% B2O3,
its mechanical strength, chemical
4% Na2O,
2% Al2O3
durability, electrical resistivity,
Softening
Temp.
821°C
and its ability to withstand therDielectric
Constant
4.6
mal stress. It is also easy to work
with and suitable for a wide
range of applications. Corning 7740 glass is offered in a choice
of diameters, in standard or thin-walled format, and either with
or without inner filament; they are additionally available in
Theta style. Single barrel glass is available in 3 lengths: 75, 100
and150 mm. Theta glass is offered in 100 mm lengths only.
BS4 64-0789
Premium Standard
Wall Borosilicate
Catalog No.
$
Model
BS4 64-0786
45.00
G100F-3 1.0
0.58
75 mm
500
G100F-4 1.0
0.58
100 mm
500
G100F-6 1.0
0.58
150 mm
500
G120F-3 1.2
0.69
75 mm
350
G120F-4 1.2
0.69
100 mm
350
G120F-6 1.2
0.69
150 mm
350
G150F-3 1.5
0.86
75 mm
225
G150F-4 1.5
0.86
100 mm
225
G150F-6 1.5
0.86
150 mm
225
G200F-3 2.0
1.16
75 mm
125
G200F-4 2.0
1.16
100 mm
125
G200F-6 2.0
1.16
150 mm
125
BS4 64-0787
BS4 64-0788
BS4 64-0790
71.00
48.00
BS4 64-0791
BS4 64-0792
39.00
BS4 64-0793
BS4 64-0794
68.00
BS4 64-0795
BS4 64-0796
49.00
BS4 64-0797
Qty/Pkg
Premium Thin Wall Borosilicate
with Filament
Premium Thin Wall Borosilicate with Filament
Catalog No.
$
Model
BS4 64-0798
45.00
G100TF-3 1.0
0.78
75 mm
500
G100TF-4 1.0
0.78
100 mm
500
G100TF-6 1.0
0.78
150 mm
500
G120TF-3 1.2
0.94
75 mm
350
G120TF-4 1.2
0.94
100 mm
350
G120TF-6 1.2
0.94
150 mm
350
G150TF-3 1.5
1.17
75 mm
225
G150TF-4 1.5
1.17
100 mm
225
G150TF-6 1.5
1.17
150 mm
225
G200TF-3 2.0
1.56
75 mm
125
G200TF-4 2.0
1.56
100 mm
125
G200TF-6 2.0
1.56
150 mm
125
BS4 64-0799
BS4 64-0800
68.00
BS4 64-0801
Premium Standard Wall Borosilicate
OD mm ID mm Length
BS4 64-0802
49.00
OD mm ID mm Length
Qty/Pkg
Catalog No.
$
Model
OD mm ID mm Length
Qty/Pkg
BS4 64-0803
BS4 64-0765
35.00
G100-3
1.0
0.58
75 mm
500
BS4 64-0804
G100-4
1.0
0.58
100 mm
500
BS4 64-0805
G100-6
1.0
0.58
150 mm
500
BS4 64-0806
G120-3
1.2
0.69
75 mm
350
BS4 64-0807
G120-4
1.2
0.69
100 mm
350
BS4 64-0808
G120-6
1.2
0.69
150 mm
350
BS4 64-0809
G150-3
1.5
0.86
75 mm
225
G150-4
1.5
0.86
100 mm
225
Premium Theta Glass
G150-6
1.5
0.86
150 mm
225
Premium Theta Glass (100 pcs/pkg.)
G200-3
2.0
1.16
75 mm
125
Catalog No.
$
Model
G200-4
2.0
1.16
100 mm
125
BS4 64-0810
76.00
TG150-4 1.5
1.0
0.2 mm
100 mm
G200-6
2.0
1.16
150 mm
125
BS4 64-0811
TG200-4 2.0
1.4
0.2 mm
100 mm
BS4 64-0766
BS4 64-0767
61.00
BS4 64-0768
BS4 64-0769
40.00
BS4 64-0770
BS4 64-0771
36.00
BS4 64-0772
BS4 64-0773
61.00
BS4 64-0774
BS4 64-0775
BS4 64-0776
47.00
44.00
68.00
49.00
Cell Biology
Capillaries with Filament
A small diameter filament is fused to the glass inside diameter to
facilitate rapid solution filling through capillary attraction. This is
especially important for the very small diameter of sharp electrodes
typically used for intracellular studies and microiontophoresis.
OD mm ID mm Septum Length
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M45
Capillary Glass
Patch Clamp Glass
Choosing patch clamp glass is generally based on noise performance and the ability to form and maintain a seal. No one type of
glass works best in all applications and some trial and error is usually required to find the type yielding optimum results in your
experiment. Patch glass is manufactured without an inner filament. This glass is offered in both the standard and premium models. The premium glass has fire polished ends and is cleaned with
deionized water before being packed in dust-free containers.
Custom 8520 Patch Glass
Premium Corning 7056 (Alkali Barium
Borosilicate) Patch Glass
Now Available in Premium Line!
Corning 7056 glass is now offered in place of the 7052 glass, last melted
in 1992 and no longer available. The 7056 formula has also been
discontinued by Corning (last melt in 1995) but stocks of this material
should be available for a few more years. The properties of the 7056 glass
are similar to the 7052 as is its performance in patch experiments.
Specialized Tools For Bioresearch
Specifications
Now Available in Premium Line!
The custom patch glass from Clark was introduced in 1997 as a substitute for the Corning 7052 glass, a favorite amongst researchers performing patch clamping. Initial tests showed the 8520 glass to be equal
to the 7052 in noise performance. More importantly, seals were
formed faster and maintained for longer periods.
Composition
68% SiO2, 18% B2O3, 9% K2O, 3% Al2O3,
1% Li2O, 1% Na2O
Softening Temperature
718°C
Dielectric Constant
5.7
Premium Corning 7056 Patch Glass
Cat. No.
$
BS4 64-0812
113.00
BS4 64-0813
Specifications
BS4 64-0814
Composition
>10% SiO2, >10% B2O3, >1% Al2O3, >1% K2O,
<1% Na2O, <1% Li2O, <1% ZnO, <1% As2O3,
<1% TiO2, <1% ZrO2
Softening Temperature
720°C
Dielectric Constant
4.6
62.00
Model
OD mm ID mm Length Qty.
G75150S-4 1.50
0.75
100 mm 225
G75150T-4 1.50
1.10
100 mm 225
G75165T-4 1.65
1.20
100 mm 190
Premium Corning 8161 (Potash Rubium
Lead) Patch Glass
Premium Custom 8520 Patch Glass
Cat. No.
$
Model
BS4 64-0817
49.00
G85150T-3 1.50
1.16
75 mm
G85150T-4 1.50
1.16
100 mm 225
G85165T-3 1.65
1.28
75 mm
G85165T-4 1.65
1.28
100 mm 190
BS4 64-0818
BS4 64-0819
41.00
BS4 64-0820
OD mm ID mm Length Qty.
225
190
This glass pulls at a lower temperature and is easily shaped. It is
recommended for low noise recordings. However, the lead content
should be considered for the intended application.
Specifications
Clark Custom 8520 Patch Glass
Composition
51% PbO, 39% SiO2, 6% K2O, 2% BaO
Softening Temperature
600°C
Dielectric Constant
8.3
Catalog No.
$
OD
ID
Length
Quantity
BS4 30-0091
52.00
1.2 mm
0.93 mm
75 mm
350
1.2 mm
0.93 mm
100 mm
350
1.2 mm
0.93 mm
150 mm
350
1.5 mm
1.16 mm
75 mm
225
Cat. No.
$
Model
1.5 mm
1.16 mm
100 mm
225
BS4 64-0815
74.00
G86150T-4 1.50
1.10
100 mm 225
1.5 mm
1.16 mm
150 mm
225
BS4 64-0816
G86165T-4 1.65
1.20
100 mm 190
1.65 mm
1.28 mm
75 mm
190
1.65 mm
1.28 mm
100 mm
190
1.65 mm
1.28 mm
150 mm
190
BS4 30-0089
BS4 30-0090
80.00
BS4 30-0094
BS4 30-0092
61.00
BS4 30-0093
BS4 30-0097
52.00
BS4 30-0095
BS4 30-0096
80.00
Premium Corning 8161 (Potash Rubium Lead) Patch Glass
OD mm ID mm Length Qty.
For Patch Clamps, see pages H2 to H5.
M46
11,000 Specialty Products to Enhance Your Bioresearch
Capillary Glass
Clark Capillary Glass
Harvard Apparatus Ltd. Acquires
Clark Electromedical Instruments
Clark Electromedical Instruments was acquired by Harvard Apparatus Ltd., Harvard Apparatus’ UK
subsidiary located in Edenbridge, England. Clark Electromedical Instruments manufactures micropipettes and micro-electrodes used primarily in intra-cellular research. Clark Electromedical will continue to trade under the Clark brand name, however, all manufacturing, marketing and sales support
has been relocated to the Harvard Apparatus Edenbridge facility.
Discounts on Quantity Puchases:
Discount apply to only single part
number quantities (no mixing).
1 to 4
none
5 to 9
5%
10+
10%
Borsilicate Capillaries
•
High quality
borosilicate glass
•
Five outside
diameters available
•
Available with either
thin or standard wall
•
Ratio of outside to
inside diameters
preserved to tip
•
Economical
The properties of borosilicate glass make it the most popular material among researchers for the fabrication of electrodes and micropipettes. Its low softening temperature combined with its mechanical
strength, chemical durability, high electrical resistivity, and its
ability to withstand thermal stress, make these capillaries the most
widely used in the world. Clark Borosilicate Capillaries are offered
in a variety of diameters and wall thickness (standard or thin
walled) with or without an inner filament. They are available in
fused multi-barrel configurations, as well as in Theta style. Single
barrel glass is available in 75 mm (3 in), 100 mm (4 in) and
150 mm (6 in) lengths.
$
OD
ID
Length
BS4 30-0037
36.00
1.0 mm
0.78 mm
1.0 mm
0.78 mm
1.0 mm
53.00
BS4 30-0049
BS4 30-0047
45.00
BS4 30-0048
BS4 30-0064
32.00
BS4 30-0065
BS4 30-0062
BS4 30-0063
45.00
Softening Temp.
815°C
Dielectric Constant
4.7
Borosilicate Standard Wall without Filament
Catalog No.
BS4 30-0036
80.9% SiO2
12.9% B2O3
4.4% Na2O
1.8% Al2O3
Borosilicate Standard Wall without Filament
Borosilicate Thin Wall without Filament
BS4 30-0035
Composition
Quantity
Catalog No.
$
OD
ID
Length
Quantity
75 mm
500
BS4 30-0018
38.00
1.0 mm
0.58 mm
75 mm
500
100 mm
500
BS4 30-0016
1.0 mm
0.58 mm
100 mm
500
0.78 mm
150 mm
500
BS4 30-0017
1.0 mm
0.58 mm
150 mm
500
1.2 mm
0.94 mm
75 mm
350
BS4 30-0043
1.2 mm
0.69 mm
75 mm
350
1.2 mm
0.94 mm
100 mm
350
BS4 30-0042
1.2 mm
0.69 mm
100 mm
350
1.2 mm
0.94 mm
150 mm
350
BS4 30-0041
1.2 mm
0.69 mm
150 mm
350
1.5 mm
1.17 mm
50 mm
225
BS4 30-0056
1.5 mm
0.86 mm
75 mm
225 .
1.5 mm
1.17 mm
75 mm
225
BS4 30-0054
1.5 mm
0.86 mm
110 mm
225
1.5 mm
1.17 mm
100 mm
225
BS4 30-0053
1.5 mm
0.86 mm
100 mm
225
1.5 mm
1.17 mm
150 mm
225
BS4 30-0055
1.5 mm
0.86 mm
150 mm
225
2.0 mm
1.16 mm
75 mm
125
2.0 mm
1.16 mm
100 mm
125
2.0 mm
1.16 mm
150 mm
125
3.0 mm
1.62 mm
75 mm
55
3.0 mm
1.62 mm
100 mm
55
3.0 mm
1.62 mm
150 mm
55
BS4 30-0073
61.00
48.00
37.00
42.00
40.00
BS4 30-0070
BS4 30-0071
64.00
BS4 30-0127
BS4 30-0080
BS4 30-0081
42.00
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
Cell Biology
Borosilicate Thin Wall without Filament
Specifications
M47
Capillary Glass
Clark Capillary Glass
Borosilicate with Filament
Standard Wall
Borosilicate Double Barrel Special
Thin Wall
•
Easy filling
•
Available in standard or thin wall configurations with
selection of three outside diameters
•
Exceptionally low tip impedance (less than 50 MΩ)
•
Good for patch clamp pipettes and microinjection needles
Specialized Tools For Bioresearch
Borosilicate with Filament
Catalog No.
$
OD
ID
Length
Quantity
Borosilicate Standard Wall with Filament
BS4 30-0034
BS4 30-0032
69.00
BS4 30-0033
BS4 30-0021
52.00
BS4 30-0019
BS4 30-0020
82.00
BS4 30-0046
BS4 30-0044
56.00
BS4 30-0045
BS4 30-0060
53.00
BS4 30-0057
BS4 30-0058
78.00
BS4 30-0076
BS4 30-0074
61.00
BS4 30-0075
BS4 30-0084
49.00
BS4 30-0082
BS4 30-0083
1.0 mm
0.50 mm
75 mm
500
1.0 mm
0.50 mm
100 mm
500
1.0 mm
0.50 mm
150 mm
500
1.0 mm
0.58 mm
75 mm
500
1.0 mm
0.58 mm
100 mm
500
1.0 mm
0.58 mm
150 mm
500
49.00
BS4 30-0038
BS4 30-0039
78.00
BS4 30-0052
BS4 30-0050
58.00
BS4 30-0051
BS4 30-0068
49.00
BS4 30-0066
BS4 30-0067
78.00
BS4 30-0128
BS4 30-0077
BS4 30-0078
57.00
Special two barrels - one barrel with filament, one without
•
Ratio of outside to inside diameter preserved to tip
Borosilicate Double Barrel Special
Catalog No.
$
OD
ID
Length
Quantity
BS4 30-0004
56.00
1.5
0.86 mm
75 mm
100
1.5
0.86 mm
100 mm
100
1.5
0.86 mm
150 mm
100
BS4 30-0005
BS4 30-0006
83.00
Borosilicate Theta Glass
0.69 mm
75 mm
350
1.2 mm
0.69 mm
100 mm
350
1.2 mm
0.69 mm
150 mm
350
1.5 mm
0.86 mm
75 mm
225
1.5 mm
0.86 mm
100 mm
225
•
Easy to fill
1.5 mm
0.86 mm
150 mm
225
•
Two channels in a single diameter
2.0 mm
1.16 mm
75 mm
125
2.0 mm
1.16 mm
100 mm
125
2.0 mm
1.16 mm
150 mm
125
3.0 mm
1.62 mm
75 mm
55
3.0 mm
1.62 mm
1.62 mm
100 mm
150 mm
55
55
Borosilicate Thin Wall with Filament
BS4 30-0040
High quality borosilicate glass
•
1.2 mm
3.0 mm
78.00
•
1.0 mm
0.78 mm
75 mm
500
1.0 mm
0.78 mm
100 mm
500
1.0 mm
0.78 mm
150 mm
500
1.2 mm
0.94 mm
75 mm
350
1.2 mm
0.94 mm
100 mm
350
1.2 mm
0.94 mm
150 mm
350
1.5 mm
1.17 mm
75 mm
225
1.5 mm
1.17 mm
100 mm
225
1.5 mm
1.17 mm
150 mm
225
2.0 mm
1.56 mm
75 mm
125
2.0 mm
1.56 mm
100 mm
125
2.0 mm
1.56 mm
150 mm
125
Borosilicate Theta Glass
Catalog No.
$
BS4 30-0116
60.00 1.5 mm
OD
BS4 30-0114
Wall
Septum Length
100
1.5 mm
0.23 mm 0.17 mm 100 mm
100
0.23 mm 0.17 mm 150 mm
100
0.30 mm 0.22 mm 75 mm
100
BS4 30-0115
117.00 1.5 mm
BS4 30-0119
2.0 mm
BS4 30-0117
96.00 2.0 mm
0.30 mm 0.22 mm 100 mm
100
BS4 30-0118
2.0 mm
0.30 mm 0.22 mm 150 mm
100
For Microforges, see pages M53 and M54.
For our full line of Pipette Pullers,
see pages M51 and M52.
M48
Quantity
0.23 mm 0.17 mm 75 mm
11,000 Specialty Products to Enhance Your Bioresearch
Capillary Glass
Clark Capillary Glass
Borosilicate Multi-Barrel
Aluminosilicate Capillaries
In recent years there has been a developing interest in fabricating
micropipettes from aluminosilicate glass. Like silicon, aluminum
combines with oxygen to form tetrhbedral networks and the Al-O bonds
are very strong. In comparison with borosilicate glass, aluminosilicate
provides increased hardness, improved chemical durability, reduced
electrical conductivity and a lower coefficient of thermal expansion.
Also, while the original ratio of a borosilicate capillary’s inner to outer
diameter will remain unchanged over its total taper length, aluminosilicate glass demonstrates a marked tendency to thin out as it is
drawn to a tip. This behavior allows extremely fine tips to be formed.
For more information, see:
1. Na/H Exchange, Vaughan-Jones, RD.; Grinstein Press, Ch.1 p.8;
2. Effects of intracellular and extracellular pH on contraction in isolated
mammalian cardiac muscle, Bountra, C. & Vaughan-Jones, R.D.; Journal of
Physiology Volume 418 (1989)
Specifications
•
High quality borosilicate glass
•
Two, three, five and seven barrel configurations with filament
•
Capillaries fused along their full length
Composition
51.9% SiO2, 22.0% Al2O3, 7.8% P2O5, 7.7% MgO,
6.9% CaO, 2.1% B2O3, 1.4% BaO and 0.2% As2O3
Softening Temperature 950°C
Dielectric Constant
6.2
Borosilicate Multi-Barrel
Catalog No.
$
OD
Wall
# of
Septum Barrels Quantity
BS4 30-0003
56.00 1.5 mm
0.86 mm 75 mm
2
100
BS4 30-0001
1.5 mm
0.86 mm 100 mm
2
100
Aluminosilicate Capillaries with Filament
83.00 1.5 mm
0.86 mm 150 mm
2
100
1.2 mm
0.69 mm 100 mm
3
100
BS4 30-0008
135.00 1.2 mm
0.69 mm 150 mm
3
100
Aluminosilicate Capillaries with Filament
BS4 30-0012
1.2 mm
0.69 mm 100 mm
5
65
Catalog No.
$
BS4 30-0013
116.00 1.2 mm
0.69 mm 150 mm
5
65
BS4 30-0110
220.00
BS4 30-0014
1.0 mm
0.58 mm 100 mm
7
60
BS4 30-0108
BS4 30-0015
107.00 1.0 mm
0.58 mm 150 mm
7
60
BS4 30-0109
424.00
OD
ID
Length
Quantity
1.0 mm
0.53 mm
75 mm
500
1.0 mm
0.53 mm
100 mm
500
1.0 mm
0.53 mm
150 mm
500
Cell Biology
BS4 30-0002
BS4 30-0007
Borosilicate Glass Rod
•
High quality borosilicate glass
•
Available in two diameters
Borosilicate Glass Rod
Catalog No.
$
OD
Length
Quantity
BS4 30-0087
74.00
1.0 mm
75 mm
500
1.0 mm
100 mm
500
1.0 mm
150 mm
500
2.0 mm
100 mm
125
BS4 30-0085
BS4 30-0086
BS4 30-0088
125.00
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M49
Pipettes
Pipette Sterilization
and Storage Container
Specialized Tools For Bioresearch
•
$
Pre-pulled
Blank
Ideal for dry heat
sterilization of pipettes
•
Pyrex-type glass covers
make contents easily
visible
•
Holds 21 pipettes
•
Available for 1.0, 1.2,
1.5 and 2 mm diameter
pipettes
This container is a convenient
and easy to use pipette storage
container. It is ideal for both storage and dry heat sterilization of
micropipettes. In most application pipettes must be free from
contamination at all times. By
storing completed pipettes and partially made pipettes (between operations), the complete cleanliness of the micropipettes can be assured.
Because the covers are made of Pyrex glass, the complete container
with pipettes in-situ can be dry-heat sterilized, ensuring contamination-free pipettes. The base accepts 21 pipettes. Select from containers
that holds either 1.0, 1.2, 1.5 or 2 mm OD pipettes.
Catalog No.
7-Barrel Iontophoresis
Micropipette
Product
BS4 69-0114
199.00 Pipette Sterilization and Storage Container for
1.0 mm Pipettes
BS4 69-0316
199.00 Pipette Sterilization and Storage Container for
1.2 mm Pipettes
BS4 69-0317
199.00 Pipette Sterilization and Storage Container for
1.5 mm Pipettes
BS4 69-0318
199.00 Pipette Sterilization and Storage Container for
2.0 mm Pipettes
Harvard Apparatus now owns Hugo
Sachs Elektronik. The HSE isolated
organ baths set the industry standard.
They can be found in the Isolated
Organ and Tissue Section K.
M50
Easy filling
•
Minimal tip resistance
•
Minimal leakage
•
Available pre-pulled or
fully-pulled
Fully-pulled
Micron tip
The MS-7P Micropipette has
been designed for iontophoretic
use as well as pneumatic ejection
of drugs. The standard blank
consists of seven barrels, pyrex
Omegadot tubing, 1.5 mm outer
diameter and 1.2 mm inner
diameter, pre-pulled to 2 mm
formation as shown. The design
of the micropipette readily lends
itself to electrical connection by
inserting Ag wire or to pressure
tube attachment for pneumatic
use. The overall length of the
blank assembly is 10 centimeters. The micropipette is supplied as
either pre-pulled blanks or fully-pulled to a micron tip as shown. The
micron tip is not bumped and therefore can be finalized prior to use.
Features
• The orifice ratio of each barrel is 0.80 and thus is optimized
for minimal tip resistance when pulled to micron size.
• Omegadot tubing permits easy filling.
• Fanned-out outer barrel assembly provides separation thus
avoiding leakage due to spills and any cross-coupling between
barrels.
• The 22 mm long twisted segment of the blank assembly
assures singular tip formation during pulling action thus
provides flexibility in a selection of a desired tip configuration.
Cat. No.
Harvard Apparatus now owns the
Navicyte line of Ussing and Diffusion
Chambers. See pages M166 to M170
for complete details on those products and many others.
•
$
Model
Micropipettes
BS4 65-0207
410.00
MS-7PB
Micropipette 7-Barrel, 1.5 mm OD
Borosilicate Glass, Pre-Pulled Blank,
pkg. of 20
BS4 65-0208
301.00
MS-7MT
Micropipette 7-Barrel, 1.5 mm OD
Borosilicate Glass, Fully-Pulled, approx.
20 µm Tip, pkg. of 10
For the Neuro Phore BH-2 System for
iontophoretic applications, see pages L4 to L8.
11,000 Specialty Products to Enhance Your Bioresearch
Pipette Pullers
Introduction to Pipettes and Pullers
Needle micropipette fabrication devices include instruments
intended to pull, bevel, or forge various types and styles of
glass micropipette needles for ICSI, IVF, transgenics, patch
recording or other similar procedures involving the use of
micropipettes in electrophysiological recording, microinjection, and microperfusion techniques.
Programmable Pipette Pullers, see page M52
Cell Biology
The first step in the process involves the initial fabrication of
the micropipette itself using either single or multibarrel
capillary tubing. This tubing typically ranges in size from
between 1 and 2 mm OD and is usually made from
borosilicate glass although other materials such as
aluminosilicate or quartz are also used in some applications.
The capillary tubing is then placed in a micropipette puller
where a point on the capillary tubing is brought to its
softening point through the use of a heating device such as an
electrical filament, laser or gas flame. Once the capillary
tubing’s softening point temperature is reached, a
mechanical parallel pulling force is applied to each end of the
capillary tube and it is ‘pulled’ to the desired tip diameter and
profile. Depending on a number of preset variables such as
capillary material type, temperature, filment type and
pulling force, numerous types of tip profiles and tip diameters
can be fabricated. Using this technique tip sizes as small as
0.02 µm can be achieved. Pipette pullers can range in
complexity from a simplistic spring/counter weight
mechanical puller to highly sophisticated programmable
microprocessor controlled pullers.
To reshape the pipette or to fuse the tip into a different
geometry, a microforge system is used. A microforge uses a
heating element, microscope, illumination, micromanipulators and microtools to change the shape of or contact fuse
other elements to the micropipette pipette by heating certain
sections of the micropipette to either soften (for bending) or
melting (fusing) the micropipette. Some types of microforges
can also be fabricate metal and glass microtools.
Harvard Apparatus, Inc. manufactures and sells the complete
range of high quality equipment and supplies to fabricate
micropipettes.
Microforges, pages M53 to M54
For Premium and Clark Capillary Glass,
see pages M45 to M49.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M51
Pipette Pullers
Programmable Pipette Pullers
Specifications
Pulling Force
Pneumatic
Multi-Barrel Pipette
4- or 7-barrel, BS4 69-0150 only
Each Barrel Pipette
1 mm OD
Heater
Nichrome coil
Heater Control
Microcontroller
Heating
74 general heat levels (24 to 99)
54 auto. heat levels (45 to 98)
Number of Sequences
25
Steps in Each Sequence 18
BS4 69-0151
BS4 69-0150
Specialized Tools For Bioresearch
Computerized Heater Control
• Two models available:
— Single-barrel
— Multi-barrel
• Microprocessor controlled system
performs programmable multiple
step pulling sequences
• Produces micropipettes for patch
clamping, intracellular electrodes,
injection micropipettes, micro
needles, etc.
• Consistent pulling force by utilizing
pneumatic pressure instead of
gravity or magnetic fields
The PMP-102 is a state of the art microprocessor controlled horizontal pipette puller
that has been designed to pull two identical
single barrel pipettes at the same time. The
pipettes can be used as patch clamp electrodes, intracellular electrodes, injection
micropipette or micro-needles.
The PMP-107 is similar to the PMP-102, but is
designed to pull a single 4- or 7-barrel pipette.
Under program control, the PMP-107 can automatically heat, twist and pull a multi-barrel
pipette. There is no need for any manual rotation or any inconsistent timing interrupt control.
Exclusive Optical-Digital Taper
Measurement
Instead of a mechanical tip length setting as on
other brands of pipette pullers, the PMP-102
and PMP-107 use an exclusive optical-digital
ruler to perform precise taper length setting,
real-time measuring and controlling. With
this feature, a user can handle the taper
pulling of pipette tips precisely and very easily.
Equipped with a powerful computerized tip
sensing function, the PMP-102 and PMP-107
can automatically finish the tip pulling.
M52
The PMP-102 and PMP-107 use an advanced
microcontroller design to perform real-time
heater monitoring and pulling control. Thus,
they will always provide precise heating power,
despite multiple/consecutive pipette pullings or
changes in thermal/electrical characteristics.
Programmable Sequences for
Creation and Reproduction
There are 25 user programmable pulling
sequences with 18 steps in each sequence.
Users can start the pulling sequence by just
pressing the Start button.
Factory Installed Preset
Programs for Major Different
Pipette Pulls
Every pipette puller is pre-installed with a
number of different pipette pulling programs.
With just a few modifications to these steps or
parameters new programs can be configured.
Taper Length Setting
0.5 to 20 mm
Pressure 1 Regulator
0.1 to 10 p.s.i.
Pressure 2 Regulator
0.1 to 60 p.s.i.
Pressure Gas Input
30 to 60 p.s.i.
Actions
Pull 1, pull 2, pull 2/cool, rotation
(BS4 69-0150 only), cool air,
return
Display
20 x 4 LCD
Power Input
110/240 VAC
Power Consumption
Max. 150 W
Dimension, H x W x D
17.8 x 27.9 x 35.6 cm
Weight:
BS4 69-0150
BS4 69-0151
6.8 kg (18 lbs)
5.9 kg (13 lbs)
Catalog No.
Programming
A simple 4x4 keypad and full information display allows the user to easily control and read
all pulling parameters. Programming functions
include sequences, steps, time, timing, heater
level, heater control, and tip length and actions.
Product
7,931.00 PMP-107 Programmable
Multi-Barrel Pipette Puller
BS4 69-0151
6,552.00 PMP-102 Programmable
Single-Barrel
Micropipette Puller
BS4 69-0154
49.00 Input Tubing with
Connector
BS4 69-0155
53.00 Output Tubing with
Connector and 4.5 ml
Vial*
BS4 69-0156
40.00 Output Tubing with
Connector Only*
Pneumatic Pulling Force
Both pullers use a precise controlled pneumatic pressure as the pulling force rather
than gravity or magnetic fields used by other
manufacturers. This technique provides more
controlled, even, and consistent dragging
characteristics allowing the units to pull an
ideal injection tip or microelectrode tip easily. A precision micro-linear ball bearing rail
and advanced pneumatic components are
used to provide no fault pulling movement.
$
BS4 69-0150
BS4 69-0157
BS4 69-0158
69.00 Foot Switch*
145.00 Remote Key Pad*
BS4 69-0160
68.00 Rack Mounting Kits
with Handles*
BS4 69-0161
20.00 Unpulled 7-Barrel Pipette
for BS4 69-0150 Only
BS4 69-0162
14.00 Unpulled 4-Barrel Pipette
for BS4 69-0150 Only
BS4 69-0165
259.00 Pipette Holder for
Single-Barrel Pipettes*
BS4 69-0166
271.00 Holder for 4-Barrel
Pipettes
BS4 69-0167
315.00 Holder for 7-Barrel
Pipettes
* Note: see page M76 for details.
These units are supplied with input tubing and
connector, spare heater filament, tweezers,
power cord and manual.
11,000 Specialty Products to Enhance Your Bioresearch
Pipette Pullers
De Fonbrune Microforge
Unit shown without
Stereo Zoom Microscope
• Fabricates microtools from simple
probes to complex micro-instruments
• Multiple fabrication techniques allow
forging/fabricating metals, alloys,
organic crystals, polymers and other
fusible materials as well as soda lime,
borosilicate glass tubing and rod stock
• Air jets provide ‘spot’ cooling of the
heating element
Detail of Electromechanical
Forge Assembly
The De Fonbrune is the oldest and most
respected name in microforging. The De
Fonbrune-type microforge is the latest in a
time-honored series based on Dr. De
Fonbrune’s original device. It complements the
precision applications of the de Fonbrune
pneumatic micromanipulator, pipette pullers
and microinjection systems by producing a
wide variety of microtools adaptable to microbiological, cytological, genetic, microsurgical,
microphysical and similar investigations and
tools as precise as your procedure. It can be
used to fabricate a wide range of microtools
employing a variety of techniques either separately or in tandem: contact fusion or melting,
distant or contact stretching, fracturing, microforging and microglass blowing. Employing a
variety of movements and temperature controls, which permit fabrication of microtools
from simple to more complex micro-instruments such as, calibrated micropipettes,
microscalpels, electrodes and micromanometers (for use in measuring devices for turgor
pressure in single cells).
This microforge is an optical-electrical forge
system. For superior stability the entire opticalelectrical forge assembly is securely bolted to
the cast aluminum main housing. This housing
contains the transformer, function switches
The electrode holder can be positioned either
vertically or horizontally and can easily be
replaced. The electrode holder and heating filament can be pivoted as a unit horizontally in a
short arc, causing the electrode to move parallel to the optical axis about 1/2 inch. Two curved
air jets are also mounted on the adjustable holder system and are equipped with an adjustable
valve. These jets direct airflow to the wire-heating filament to provide ‘spot’ cooling of the heated element. Adjusting the current to the electrode controls filament heat. The heat at a given
setting will vary depending on the size and type
of filament wire, the amount of air blown against
the filament and the amount of heat conducted
away from the filament by the tool.
Cell Biology
• Combines precise control of both
movement and temperature
and the operating controls for the adjusting filament temperature and light intensity. A circular disk, or mechanical stage, is mounted in
front of the lamp housing. A pivoting spindle
cylindrical vice with clamp for supporting the
microtool being made is mounted at the
periphery of this disk. Both disk and spindle
may be rotated about their own axes and precision screws can adjust the entire assembly vertically or horizontally. The light intensity directed to the microscope objectives may also be
adjusted. The electrical forge sub-assembly is
mounted on an adjustable electrode holder system directly in front of the microscope objectives and to the left of the microtool being fabricated. The spring-loaded filament holder
provides for easy insertion or replacement of
the platinum-iridium (or other filament material) forge element. The vertical and horizontal
position of the heating element can be precisely controlled by the two controls mounted on
the operating head and main electrode casting.
Two controls on the stage are used to position
the vertical and horizontal movement of the
microtool.
The system is supplied with platinum/iridium
wire for the electrode and has the ability to utilize other types and gauges of forging filaments. The apertures of the electrode pins will
accommodate up to 22 gauge (0.0253 inch
diameter) filament wires. The following items
are also included with the system: 4 microtool
reamers, wax inducing instrument, capillary
glass tubing, hammering rod, 3 sticks sealing
cement, 4 80 mm microtool holders, 15-watt
bulb, weight set (0.5 to 17 gm in 1 gm increments), 31 ga heating element wire, 1 pkg. 2 x
150 mm pyrex glass rod, forceps and 2 angular microtool holders.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M53
Pipette Pullers
De Fonbrune Microforge
Specialized Tools For Bioresearch
Fabrication Examples
A. Cupping glass:
Bent at an angle for
positioning in a moist
chamber.
B. Three types of
cupping glasses:
(a) rounded tip,
(b) rounded tip slightly
constricted, (c) blown
cupping glass.
C. Orifices of capillary
tubes: (a) Sharp edge,
(b) Smooth edge,
(c) Constricted orifice.
D. Local constrictions
of a capillary tube:
A circular (a) or
elongated (b) orifice
is formed within the
capillary.
E. Spatula: Piece of
triangular glass whose
corners have been
fire polished and then
affixed to a glass rod.
F. Various types of
micro-needles; with
plain and pediculated
points. Hook and
needle with eye.
G. Retaining hook.
H. Different types of
micro-pipettes: (a, b, c)
terminal orifice;
(d and e) lateral orifice.
I. Piercing a Glass Wall:
a, a’, projecting tips;
b, b’, flat tip; c, passage
of a metal wire through
an aperture as a’; then
sealing it by fusion.
A flat plane may be perforated in the same
manner.
J. Type of Scalpel: A
small globule of glass
is affixed to filament.
Then a platinum wire
embedded in a glass
capillary enters the
surface of the heated
glass. The heat is
turned off; instrument
pulled away from a
circular cutting edge.
K. Spatula loop: Light
coming through the
opening is darkened
by a small film of
transparent lacquer.
L. Micro-gauge: The
horizontal portion of
this gauge is made
with a hollow cylinder.
M. Probe (a) and
cannula (b).
Specifications
Catalog No.
Tool Movement:
Vertical
Horizontal
BS4 69-0384
6,242.00 MF-1-115 Microforge without Optics, 115 VAC, 60 Hz
12.7 mm (~0.5 in)
10.1 mm (~0.4 in)
BS4 69-0438
6,242.00 MF-1-230 Microforge without Optics, 230 VAC, 50 Hz
Electrode Movement:
Vertical
Horizontal
Focus
7.6 mm (~0.3 in)
15.2 mm (0.6 in)
12.7 mm (~0.5 in)
Model
Product
Weight
Dimensions, H x W x D
Power
Replacement Parts
BS4 69-0385
70.00 MF-2
Electrode Filament Holder
BS4 69-0387
13.00 MF-4
Hammering Rod, SS 3/16 x 3.75 in
(95 mm) (D x L)
17.25 kg (38 lbs)
BS4 69-0388
46.00 MF-5
Bulb, Frosted for Microforge Illuminator
38.1 x 27.9 x 40.6 cm (15 x 11 x 16 in), without optics
BS4 69-0389
69.00 MF-6
Tool Holder Clamp
100/120 VAC, 60 Hz, 220/240 VAC, 50 Hz
BS4 69-0390
3.00 MF-7
BS4 69-0391
20.00 MF-8
Filament Wire, Platinum, 20% IRID, 102
mm (4 in) L, 31 ga, 229 µm (0.009 in) OD
BS4 69-0392
25.00 MF-9
Filament Wire, Platinum, 20% IRID, 102
mm (4 in) L, 30 ga, 305 µm (0.012 in) OD
Harvard Apparatus now
owns Clark Electromedical.
See pages M47 to M49 for
the industry standard capillary glass.
M54
$
Fuse, ACG 5, 1/4 x 1-1/4 in
BS4 69-0448
41.00 AK15
BS4 69-0394
238.00 MF-11
Accessory Kit without weights
Glass head pipettes, 3 mm OD, pkg of 100
BS4 69-0395
633.00 MF-12
Filament Holder Assembly with Center
Rod, 6.4 x 51 mm (0.25 x 2 in) (OD x L)
11,000 Specialty Products to Enhance Your Bioresearch
Electroporation Products
MOS 25 & MOS 96 Advanced Electroporation Systems
Catalog No.
•
Multi-well plates can be electroporated in less than 10
minutes!
•
Integrated electrodes
•
Fast and precise molecular delivery
•
Rapid and easy experimental optimization
•
High throughput approach to experimentation
•
Multiple experiments in the sample plate
•
Rapid analysis - in a matter of minutes research 96
experimental parameters
•
Disposable - For ease-of-use and no carryover
Description
7,845.00 MOS 25/200 MOS 25/200 System, includes ECM™
830 Generator, 6 x 25-Well Optimizer
Plates, HT-200 Plate Handler and
instructions
BS4 45-0411
9,265.00 MOS 25/100 MOS 25/100 System, includes ECM™
830 Generator, 6 x 25-Well Optimizer
Plates, HT-100 Plate Handler and
instructions
BS4 45-0420
7,845.00 MOS 96/200 MOS 96/200 Advanced
Electroporation System, includes
ECM™ 830 Generator, 2 x 96-Well
Advanced EP Plates, HT-200 Plate
Handler and instructions
BS4 45-0421
9,265.00 MOS 96/100 MOS 96/100 Advanced
Electroporation System, includes
ECM™ 830 Generator, 2 x 96-Well
Advanced EP Plates, HT-100 Plate
Handler and instructions
BS4 45-0452
150.00 HT-P96-4
BS4 45-0462
50.00 HT-P25-4
96-Well Disposable Electroporation
Plate, with clear bottom, 250 µl
capacity per well, 4mm gap
25-Well Disposable Optimizer Plate,
with clear bottom 250 µl capacity per
well, 4mm gap
BS4 45-0401
3,495.00 HT-200
Multi-Well Plate Handler with AutoSense Track Switching, includes connectors for standard BTX pulse generators
BS4 45-0400
1,995.00 HT-100
Multi-Well Plate Handler with Manual
Track Switching, includes connectors
for standard BTX pulse generators
BS4 45-0002
6,615.00 8300
ECM™ 830 Square Wave
Electroporation System, see pages
M56 and M57
Applications Include:
•
Transfection of Mammalian Cells
•
Transformation of Bacteria and Yeast
•
cDNA Library Screening
BS4 45-0052
6,077.00 ECM830
ECM™ 830 Generator Only
•
Drug Screening
BS4 45-0001
5,299.00 6300
ECM™ 630 Exponential Decay Wave
Electroporation System, see pages
M58 and M59
BS4 45-0051
4,761.00 ECM630
ECM™ 630 Generator Only
The Future of Electroporation Is Here!
BTX, the leader in electroporation products and specialty electrodes,
has developed a new and innovative product line for experiment
optimization and large volume electroporation. The Advanced
Electroporation System is a breakthrough in Molecular Delivery. The
days of researcher’s struggling with tedious, multi-step procedures
that typically limited the number of samples and cell types they could
test are all about to change.
Cell Biology
Methods optimization
Model
BS4 45-0410
Features and Benefits
•
$
The Methods Optimization Systems, MOS 25 & MOS 96, allow you to
boost your productivity by optimizing your conditions and increasing
your yields and viability. The MOS 25 system takes less then 5 minutes to run while the MOS 96 less then 10 minutes! These systems
allow the user to quickly and efficiently optimize the electrical and
biological parameters associated with any electroporation experiment. Now entire libraries can be screened rapidly and efficiently.
Both the MOS 25 and MOS 96 Advanced Electroporation Systems
come complete with disposable multi-well Optimizer Plates, a Plate
Handler and an ECM™ 830 Generator, see pages M56 and M57 for
complete details on the generator.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M55
Electroporation Products
Specialized Tools For Bioresearch
MOS 25 & MOS 96 Plate Handlers
To complete the MOS 25 Systems there are two plate handlers available, HT-200 and HT-100. The advanced HT-200 Plate Handler features Auto-track sensing which automatically switches columns in the
Multi-well plate during electroporation. The Auto-track sensing allows
rapid electroporation of an entire plate by setting up the electrical
parameters for all columns and pressing one button. The HT-200 is
ideal for a busy research laboratory running many daily samples.
The Model HT-100 requires manual switching from column to column and is ideal for laboratories doing basic electroporation
research or fewer samples. Both accept either the 96-Well or 25Well Optimization Plates and provide a safe and reliable working
area for processing the Electroporation Plates. Both Plate Handlers
have pulse switching technology integrated into the package and gold
plated contacts to mate with the Electroporation Plate. The Plate
Handlers were designed to operate in space constrained areas such
as laminar flow hoods.
Catalog No.
BS4 45-0401
BS4 45-0400
$
Model
3,495.00 HT-200
1,995.00 HT-100
Description
Multi-Well Plate Handler with AutoSense Track Switching, includes connectors for standard BTX pulse generators
Multi-Well Plate Handler with Manual
Track Switching, includes connectors
for standard BTX pulse generators
NEW
ECM® 830 Square Wave
Electroporation System
•
Superior square wave technology and bipolar pulsing lead to
higher viability and efficiency
•
Low voltage mode 1 V discrimination and 0.5% accuracy
allows for greater reproducibility for low voltage applications
such as In Vivo gene therapy, In Ovo gene transfer and
nuclear transfer applications
•
Monitoring of voltage, pulse duration, pulse interval and
pulse number allows for optimization of protocols, troubleshooting and documentation of parameters
•
Arc quenching™ minimizes damage caused by arcing
•
Generator will not be damaged in the event of a short circuit
•
External safety stand protects generator from damage
caused by spills or splashes
•
All digital user interface design allows for simple operation
•
Single rotary dial control simplifies parameter settings
•
Voltage Range of 5 V to 3000 V is the basis for a generator
capable of electroporating ALL cell types
•
Pulse duration range of 10 µs to 10 sec gives unparalleled
flexibility for varied applications and optimization range
•
Footswitch compatible for hands-free operation
The ECM 830 is a Square Wave Electroporation System designed for all
in vitro and in vivo electroporation applications. The generator utilizes
the BTX Power Platform Technology and a digital user interface. The
ECM 830 possesses key features including finer voltage discrimination
and 0.5 V accuracy, Arc Quenching™, the monitoring of all key parameters, and the control of pulse intervals.
Applications
Mammalian Cell Transfections/Gene Therapy
The use of square wave pulses for the transfection of mammalian cells is
well established. Fakhrai has used a BTX 800 Series in the technique of
Electro-Immuno Therapy.1 The ECM 830 will enable transfection of
mammalian cells while maintaining higher cell viability.2 For many
mammalian cells, higher transfection efficiencies will also be obtained.3
Mammalian Cell Protein/Drug Electroincorporation
Call for your local BTX Dealer
or visit www.btxonline.com.
M56
Square wave pulses have been used to electroincorporate a variety of
molecules into mammalian cells; Tsong has used square wave to load
drugs into erythrocytes4 while Marrero has used a BTX 800 Series and
BTX Model 366 Petri Dish Electrode to incorporate biologically active
antibodies into RASM cells.5
11,000 Specialty Products to Enhance Your Bioresearch
Electroporation Products
ECM® 830 Square Wave Electroporation System
In Vivo Applications
Recent advances by BTX have allowed researchers to perform in vivo
electroporation. In vivo Electro Gene Therapy has significant advantages
over viral mediated gene transfer, biolistics, and the use of cationic
Lipids.6,7,8
Nuclear Transfer
BTX square wave systems have been used by the experts in mammalian
embryo manipulation techniques in nuclear transfer for applications
ranging from pharmaceutical production to organ generation.9,10,11
Plant Applications
Intact plant tissue and protoplasts may be transformed using electroporation,12 and references indicate that square wave is indeed superior to
exponential decay for plant protoplast transformation.13
Specifications
Operational Status
Internal self test upon start-up
Interface
Digital User Interface
Input
110 V/220 V Universal
Charge Time
5 sec maximum (without delay)
Voltage Range:
LV Mode
HV Mode
5 to 500 V / 1 V resolution
30 to 3000 V / 5 V resolution
Pulse Length Range:
LV Mode
LV Mode
LV Mode
HV Mode
10 µsec to 999 µsec / 1 µsec resolution
1 msec to 999 msec / 1 msec resolution
1 sec to 10 sec / 0.1 sec resolution
10 to 600 µsec/1 µsec resolution
Voltage dependent/internally controlled
Multiple Pulsing
1 to 99
Bacterial and Yeast Electroporation
Pulse Interval
100 msec - 10 sec
The ECM 830 is capable of reproducing 800 Series bacterial protocols,
including all four E. coli electrotransformation protocols referencing
transformation efficiencies equivalent or superior to exponential decay
results.14 Square wave pulses are used to transform yeast species at high
efficiencies relative to exponential decay electroporation.15
Programmability
Storage for 2 setups (V,t,n,interval)
Arc Control
Arc Quenching™
Safety
Generator short circuit proof
Capacitance
4000 µF
Amperage
500 A limit at 10 msec
This product meets the requirements of the European Communities
(EC), and complies with EMC Directive 89/336/EEC and 73/23/EEC
for product safety.
Dimensions, H x W x D 14 x 31.8 x 31.1 cm (5.5 x 12.5 x 12.25 in)
Weight
15 lbs (6.8 kg)
Display
20 x 4 character LCD
Controls
Single Rotary Encoder with push button toggle between all set
parameters. Additional on/off Power and Start switches
References
Fakhrai, H. et al., PNAS, 93: 2909- 2914 (1996)
Monitoring
Monitoring and display of V, t, n, interval
2.)
Pasco, David, University of Mississippi, Natural Products Center, Personal
Communication and Protocols PR0296- 0298, PR0345-349, PR0351-355 (1998)
Remote Operation
Footswitch available
3.)
Takahashi, M. et al., Leukemia Research, 15 (6): 507-513 (1991)
Warranty
2 Years
4.)
Tsong, T., et al., Biblthca Haemat, 51: 108-114 (1985)
5.)
Marrero, M., et al., The Journal of Biological Chemistry, 270 (26): 15734-15738
(1995)
Catalog No.
6.)
Miramatsu et al., International Journal of Molecular Medicine, 1: 55-62 (1998)
BS4 45-0052
7.)
Nishi, T., et al., Cancer Research, 56: 1050-1055 (1996)
BS4 45-0002
8.)
Heller, R., et al., FEBS Letters, 389: 225-228 (1996)
9.)
Cambell, K., PPL Therapeutics, Roslin, Scotland, Personal Communication (1998)
10.) Cibelli, Jose et al., Science, 280: 1256-1258 (1998)
11.) Meng, J., et al., Biology of Reproduction, 57: 454-459 (1997)
12.) Lin, C., et al., Plant Physiol. Biochem., 35 (12): 959-968 (1997)
13. ) Saunders, J., Guide to Electroporation and Electrofusion, 227-247 (1991)
$
Model
Product
6,077.00 ECM830
ECM 830 Generator (Only)
6,615.00 8300
ECM 830 Electroporation System.
Includes ECM 830 Generator (BS4
45-0052), Cuvettes 1 mm, 2 mm, 4
mm, pack of 30 (10 each), Cuvette
Safety Stand (BS4 45-0207), and
Cuvette Rack (BS4 45-0208)
Cell Biology
1.)
14.) BTX Protocols PR0182, PR0183, PR0336, PR0342 (1998)
15.) Costaglioli, P., et al., Current Genetics, 27 (1): 26-30 (1994)
Call for your local BTX Dealer
or visit www.btxonline.com.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M57
Electroporation Products
Specialized Tools For Bioresearch
NEW
M58
ECM® 630 Exponential Decay Wave Electroporation System
•
Precision pulse™ provides more reliable and reproducible results
•
Over 8300 RC time constants in low voltage mode allows
unparalleled performance for precise optimization of plant and
mammalian protocols
•
126 RC time constants in high voltage mode allows unparalleled performance for precise optimization of bacteria and yeast
protocols
•
Low voltage mode: 1 V discrimination allows for greater reproducibility for low voltage applications, including mammalian cell
transfections
•
6000 A limit low voltage mode/3000 A limit high voltage mode
allows for unparalleled power to drive exclusive BTX specialty
electrodes
•
Monitoring of peak voltage and time constant for optimization
of protocols, troubleshooting and documentation of parameters
•
Generator will not be damaged in the event of a short circuit
•
External safety stand protects generator from damage caused
by spills
•
All digital user interface design allows for improved operation
•
Single dial control simplifies parameter settings
•
Voltage range of 10 to 2500 V is the basis for a generator capable of electroporating all cell types
•
Unparalleled resistance range of 25 to 1575 Ω in 25 Ω increments and discrimination in both LV and HV
•
Resistance setting on “None” in LV mode reproduce protocols
from competitive electroporators with no resistor settings or
“unlimited” resistance
•
Capacitance Range of 1 µF, 25 µ F to 3275 µF is the widest
capacitance range available for low voltage mode applications
•
Approximately 400 BTX Protocols simplify electroporation steps
•
ECM database with approximately 4000 reference papers give
researchers the data needed to develop their own protocols
The ECM 630 is a state of the art exponential decay wave electroporation system designed for in vitro and in vivo electroporation applications. This system incorporates features that make it the most
technically advanced exponential decay electroporation system currently available. The generator utilizes the new BTX Power Platform
Technology design and novel digital user interface. The revolutionary Precision Pulse™ System provides the researcher unparalleled
power in controlling the time constant. With the ability to deliver a
maximum of 6000A in the Low Voltage Mode, the ECM 630 is the
most powerful generator in its class.
Over 200 ECM 600 protocols may be duplicated with this instrument.
The additional ECM 630 resistor selection “none” will allow
researchers to reproduce protocols from competitive systems lacking resistor settings or reporting “unlimited” resistance. Indeed, the
ECM 630 will perform the widest range of electroporation applications among commercial electroporators on the market.
Application
Mammalian Cell Transfections/Protein/Drug
Electroincorporation
Long duration electroporation, consistent with the ECM 630 platform, has resulted in higher expression levels in COS-7, EDR3,
DG44, and CV1 cell lines.1 The ECM 630 will duplicate the five-fold
increase in transfection efficiency in comparison to calcium phosphate precipitation for COS-7 reported by Ishmael et al.2 as well as
the murine T Lymphoma results reported by Sundback et al.3
Bacterial and Yeast Electroporation
The ECM 630 is the most flexible bacterial and yeast electroporator
available and will reproduce protocols by Beggs et al. for
Mycobacterium avium;4 Sanders et al. for Haemophilus influenza and Haemophilus somnus;5 and Faber et al. for Hansenula
polymorpha.6
11,000 Specialty Products to Enhance Your Bioresearch
Electroporation Products
ECM® 630 Exponential Decay Wave Electroporation System
Plant Applications
Intact plant tissue and protoplasts may be transformed using the ECM
630, including the species Agrostis palustris (creeping bentgrass) and
Lycopersicon esculentum (tomato),7 as well as tobacco pollen transformation.8
Other Cell Types
Exponential decay electroporation has been demonstrated to be
superior to microinjection for medaka oocytes.9 Yee et al. transfected and expressed firefly Luciferase in Giardia lamblia.10 In addition, Quillan et al. electrotransfected Xenopus laevis melanophores
and fibroblasts.11
Specialty Electrode Applications: The BTX
Choice
Takacs et al. directed the electroporation of COS-7 in a 96-well plate
format,12 while Parham et al. optimized transient gene expression in
CHO, CV-1, COS-7, HEK 293, and NSO.13
Specifications
Operational Status
Internal self test upon start-up
Interface
Digital User Interface
Input
110 V/220 V Universal
Charge Time
5 sec maximum
Voltage Range:
LV Mode
HV Mode
10 to 500 V / 1 V resolution
50 to 2500 V / 5 V resolution
Capacitance Range:
LV Mode
HV Mode
1 µF, 25 µF to 3275 µF /25 µF resolution
25 µF, 50 µF
Resistance Range:
HV & LV Modes
LV Mode
25 Ω-1575 Ω / 25 Ω resolution
“None” setting to simulate alternative systems lacking resistance control
Precision Pulse™:
LV Mode
HV Mode
Over 8300 RC Time Constants
126 RC Time Constants HV Mode
Safety Short Circuit Proof Maximum Pulse Delay 10 sec
Applications
Exponential decay generators may be used for a variety of animal in
vivo applications, including drug delivery to pig carotid artery14 and
transdermal delivery of large molecules.15
This product meets the requirements of the European Communities
(EC), and complies with EMC Directive 89/336/EEC and 73/23/EEC
for product safety.
References
1.)
Bodwell et al., Journal of Steroid Biochemistry and Molecular Biology, 68: 77-92
(1999)
Ishmael, et al., Journal of Neurochemistry, 67 (4): 1500-1510 (1996)
3.)
Sundback et al., Journal of of Immunology, 5971-5978 (1998)
4.)
Beggs et al., Journal of Bacteriology, 177 (17): 4836-4840 (1995)
5.)
Sanders et al., FEMS Microbiology Letters, 154 (2): 251-258 (1997)
6.)
Faber et al., Current Genetics, 25 (4): 305-310, (1994)
7.)
Lin et al., Plant Physiology and Biochemistry, 35 (12): 959-968 (1997)
8.)
Smith et al., Plant Science, 104: 49-58 (1994)
9.)
Chen et al., Zoological Studies, 34 (4): 215-234 (1995)
10.) Yee, and Nash, T.E., PNAS, 92 (12): 5615-5619 (1995)
11.) Quillan et al., PNAS, 92: 2894-2898 (1995)
6000 A limit
3000 A limit
Dimensions, H x W x D 14 x 31.8 x 31.1 cm (5.5 x 12.5 x 12.25 in)
Weight
10 lbs (4.5 kg)
Display
20 x 4 character LCD
Controls
Single Rotary Encoder with push button toggle between all set
parameters. Additional on/off Power and Start switches
Monitoring
Monitoring and display of Vp and RC Time Constant
Warranty
2 Years
Catalog No.
$
Model
Product
BS4 45-0051
4,761.00 ECM630
ECM 630 Generator Only
BS4 45-0001
5,299.00 6300
ECM 630 Electroporation System,
Includes ECM 630 Generator (BS4
45-0051), Cuvettes 1 mm, 2 mm, 4
mm, pack of 30 (10 each), Cuvette
Safety Stand (BS4 45-0207), and
Cuvette Rack (BS4 45-0208)
Cell Biology
2.)
Amperage:
LV Mode
HV Mode
12.) Peterfy et al., methods in Molecular and Cellular Biology, 5 (6): 353-362 (1995)
13.) Parham et al., Cytotechnology, 28: 1-9 (1999)
14.) Cui, Journal of the American College of Cardiology, 29: 201a (1997)
15.) Zhang, et al., Bioelectrochemistry and Bioenergetics, 42: 283-292 (1997)
Call for your local BTX Dealer
or visit www.btxonline.com.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M59
Electroporation Products
Specialized Tools For Bioresearch
NEW
ECM® 399 Electroporation System
The ECM 399 is an electroporation system specifically designed to produce the
precise field strengths and pulse lengths required for the transformation of bacterial and yeast cells and transfection of some mammalian cells. The ECM 399
utilizes the BTX Power Platform Technology design and novel digital user interface. The single-dial encoder simplifies the operation of ECM 399. Portable, convenient, and user-friendly are major features of this unit.
Applications
Bacteria/Yeast Transformation
In the High Voltage (HV) Mode, the ECM 399 is optimized to electroporate E. coli
and other bacteria and yeast for construction of cDNA libraries or mutagenesis.
Mammalian Cell Transfection
In the Low Voltage (LV) Mode, the ECM 399 is designed to deliver a pulse length
of approximately 26 msec using the 4mm gap cuvette, at a volume of 400 µl using
conductive media such as PBS. This protocol has been found to be ideal for
transfection of lymphoid, myeloid, glial, fibroblast and Cos 7 cells.1 Protocols for
these and other mammalian cells can be optimized by varying the voltage and/or
the pulse length. Adjusting the voltage adjustment knob varies the voltage, while
the pulse length may be changed by varying the sample volume or buffer composition.
This product meets the requirements of the European Communities (EC), and
complies with EMC Directive 89/336/EEC and 73/23/EEC for product safety.
“Single-Dial” control is easy to learn and easy
to use
•
Simple three-step operation: switch on, dial-in
voltage and deliver pulse
•
LCD digital read-out allows for high resolution
display of test parameters
•
Displays pulse length and peak voltage that
facilitates optimization and troubleshooting
•
High and low voltage modes for bacterial and
mammalian cells
•
Personal cuvette holder is color-coded which
reduces the potential for cross contamination
•
Compact size saves space and is portable
•
Cost effective – an excellent alternative to
chemical transfection
Specifications
Operational Status
Internal Self Test Upon Start-up
Interface
Digital User Interface
Charge Time
5 sec maximum
Voltage Range:
LV Mode
HV Mode
2 to 500 V / 2 V resolution
10 to 2,500 V /10 V resolution
Capacitance:
LV Mode
HV Mode
1050 µF
36 µF
Resistance:
LV Mode
HV Mode
150 Ω
150 Ω
Safety
Short circuit and arc proof
Dimensions, H x W x D 10.9 x 23.1 x 19.6 (4.3 x 9.1 x 7.7 in)
Weight
7 lb
Display
16-character LCD backlit
Controls
Single rotary encoder for voltage selection.
Additional on/off power and start switches
Monitoring
Monitoring and display of Vp and RC time constant
Warranty
2 Years
Catalog No.
Reference
1.)
•
C. Baum et al., "An Optimized Electroporation Protocol Applicable to a Wide Range of Cell Lines",
BioTechniques vol. 17(6): 1058-1062, (1994)
$
Model
1,764.00 ECM399
ECM 399 Generator
Only
BS4 45-0000
1,928.00 399
ECM 399 Electroporation
System, Includes ECM
399 Generator (BS4 450050), Cuvettes 1 mm,
2 mm, 4 mm, pack of
30 (10 each), PEP-BLU
(BS4 45-0212), and
Cuvette Rack
(BS4 45-0208)
BS4 45-0212
82.00 PEP-BLU
Call for your local BTX Dealer or visit www.btxonline.com.
M60
Product
BS4 45-0050
11,000 Specialty Products to Enhance Your Bioresearch
Personal
Electroporation Pak
"plug-in" cuvette module
Electroporation Products
NEW
ECM® 2001
Specifications
AC Parameters
(Alignment)
Proprietary Non-Sinusoidal Wave Shape
The ECM 2001 is a versatile
electro cell manipulation instrument. It generates a proprietary
AC wave form for benign dielec• Microprocessor
trophoretic alignment of cells.
Controlled Precision
With only microsecond switch• Wide Range of
over time from AC to DC, effiParameters
cient fusion can take place. After
• Remote Control
fusion, AC reapplication keeps
cells together for the rounding
off process. The DC square pulse section can be used as a stand
alone powerful electroporation system with a wide range of voltages
and pulse lengths capable of operating into low impedance loads.
•
Versatile Electro Cell
Fusion & Electroporation
Instrument
The 3000 Volt DC square wave pulse delivers a high voltage square
wave electroporation pulse. All 800 Series protocols can be used
with this system. The researcher will own two complete systems
(electroporation and electrofusion in one) making the ECM 2001
more versatile. A pulse length of 1 - 99 µsec allows for an extensive
range of electroporation pulse lengths. The ECM 2001 is the only
square wave electroporation system commercially available that
allows this range of pulse lengths. The researcher can optimize the
experimental conditions to obtain the best results possible.
Post fusion AC pulse allows for compression of the cells following
application of the electrofusion pulse. Hybrids do not fall apart,
resulting in a higher number of hybrids. The manual start allows for
real time visual optimization to obtain maximum number of hybrids.
Best results are obtained for fusing blastomere to oocyte for embryo
cloning. The automatic start allows the researcher to start the alignment, fusion and compression process by pressing a single button. A
remote control allows easy operation of the instrument from outside
the hood, while the number of repeats allows for expansion of
parameters.
1 MHz
Voltage (zero to peak)
0 to 75 V
Duration
0 to 99 sec
Post Fusion AC
1/10 of prefusion
DC Pulse Parameters
(Fusion/Electroporation)
High Voltage Mode
Voltage
Pulse Length
10 to 3000 V
1 to 99 µsec
Low Voltage Mode
Voltage
Pulse Length
10 to 500 V
0.01 to 0.99 msec
General
Amplitude
1 to 99 msec
Post Fusion Ramp
1 to 9 sec
Pause between AC / DC
50 µsec
Number of Pulses
1 to 99
Sequence
Automatic or manually controlled
Number of Sequence Repeats
1 to 9
Dimensions, H x W x D
27.9 x 43.2 x 39.4 cm (11 x 17 x 15.5 in)
Warranty
2 years
Power
100/115 VAC, single phase
230 VAC, single phase
Fuse
7A slow blow
Weight
47 lbs
Catalog No.
$
Model
Product
BS4 45-0053
12,093.00 ECM2001 ECM 2001 Cell Fusion/Embryo
Manipulator Generator Only
BS4 45-0003
12,872.00 2001EP
ECM 2001 Electroporation System,
Includes 2001 Generator (BS4 45-0053),
Cuvettes 1 mm, 2 mm, 4 mm, pack of 30
(10 each), Cuvette Safety Stand (BS4 450207), and Cuvette Rack (BS4 45-0208)
BS4 45-0005
13,271.00 2001EM
ECM 2001 Embryo Manipulation System.
Includes 2001 Generator (BS4 45-0053),
Microslides (BS4 45-0103, BS4 45-0104,
BS4 45-0105), Micrograbber Cables (BS4
45-0200, BS4 45-0201), and Coaxial
Connection Cable (BS4 45-02103)
BS4 45-0007
15,234.00 2001EF
ECM Electro Cell Fusion System. Includes
2001 Generator (BS4 45-0053),
Microslides (BS4 45-0103, BS4 45-0104,
BS4 45-0105), Micrograbber Cables (BS4
45-0200, BS4 45-0201), Meander Fusion
Chamber (BS4 45-0107), Flat
Electrode/Divergent Field (BS4 45-0108),
Electrode Adapter (BS4 45-0202), Coaxial
Connection Cable (BS4 45-02103),
Electroporation chambers, Cuvettes 1 mm,
2 mm, 4 mm, pack of 30 (10 each),
Cuvette Safety Stand (BS4 45-0207), and
Cuvette Rack (BS4 45-0208)
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
Cell Biology
The 1 MHZ AC pulse aligns cells in real time and saves time (faster)
in comparison to PEG. Electrofusion process takes seconds, PEG
takes hours. Researchers can change parameters (voltage or time)
to get the maximum number of hybrids aligned in “real time.” This
helps in the alignment of blastomere to oocyte at the correct position
for better fusion accuracy for embryo cloning. The researcher is able
to watch the fusion process (under microscope) which makes optimization much easier.
Frequency
M61
Electroporation Electrodes
NEW
Genetrodes™
Specialized Tools For Bioresearch
Specifications
Voltage Range
0 to 200 V
Pulse Length Range
1 µ sec - 99 msec in PBS
In Vivo Genetrodes
In Vivo Genetrode tips are invasive, gold coated
and 0.5 mm in diameter. The shafts are straight
and 0.8 mm in diameter
Model 508:
Electrode Length
Shaft Configuration
5 mm
long
The BTX Genetrodes are needle-style reusable electrodes that are used
for a variety of applications. Genetrodes come in five models, with each
model consisting of a pair of electrodes. The Model 508 and 510
Genetrodes are straight electrodes with gold tips. The Model 512, 514,
and 516 Genetrodes are bent L-shaped electrodes with gold tips. These
electrodes are connected to a Model 465 Square-Post Micrograbber
Cable and interfaced with a pulse generator via a Model 5343 BananaCoaxial Cable. The Genetrodes may be positioned in parallel at a predetermined gap within a tissue using the Model 515 Genetrode Holder.
Model 510:
Electrode Length
Shaft Configuration
10 mm
long
General Applications
Genetrode Holder Interelectrode Gaps 1 to 10 mm in 1 mm increments
Genetrodes may be used for many in vivo and in ovo electroporation
applications, including drug and gene delivery. The electrodes are
placed into a tissue following injection of the molecule of interest, and
an electroporation pulse is delivered using a BTX Electroporation
System, such as the ECM 830, 2001, and 630. The electric field introduced by the Genetrodes causes transient pores to form in the cells of
the tissue, allowing uptake of the molecules into the cells. Genetrodes
may be cleaned with a mild detergent and sterilized with ethanol or ethylene oxide. Properly maintained Genetrodes have a life span of approximately 1500+ pulses and are compatible with most BTX
Electroporation Systems.
In Ovo Genetrodes
In Ovo Genetrode tips are non-invasive, gold
coated and 0.5 mm in diameter. The shafts are
0.8 mm in diameter and L-shaped
Model 512, Electrode Length
5 mm
Model 514, Electrode Length
3 mm
Model 514, Electrode Length
1 mm
Generator Compatibility
ECM 830, 2001 and 630
Catalog No.
$
Model
BS4 45-0113
225.00 508
Straight Electrode 5 mm Gold Tip
BS4 45-0114
255.00 510
Straight Electrode 10 mm Gold Tip
BS4 45-0115
255.00 512
Bent, L-shaped Electrode 5 mm Gold Tip
BS4 45-0116
255.00 514
Bent, L-shaped Electrode 3 mm Gold Tip
BS4 45-0117
255.00 516
Bent, L-shaped Electrode 1 mm Gold Tip
BS4 45-0203
265.00 515
Genetrode Holder
BS4 45-0201
50.00 465
Square-Post Micrograbber Cables
BS4 45-0210
97.00 5343
Banana-Coaxial Connection Cable
BS4 45-0160
667.00 508 KIT
Includes Straight 5 mm Electrode Gold
Tip (BS4 45-0113), Holder (BS4 450203), and Cables (BS4 45-0201 and
BS4 45-0210)
BS4 45-0161
667.00 510 KIT
Includes Straight 10 mm Electrode
Gold Tip (BS4 45-0114), Holder (BS4
45-0203), and Cables (BS4 45-0201
and BS4 45-0210)
BS4 45-0162
667.00 512 KIT
Includes Bent, L-shaped 5 mm
Electrode Gold Tip (BS4 45-0115),
Holder (BS4 45-0203), and Cables
(BS4 45-0201 and BS4 45-0210)
BS4 45-0163
667.00 514 KIT
Includes Bent, L-shaped 3 mm
Electrode Gold Tip (BS4 45-0116),
Holder (BS4 45-0203), and Cables
(BS4 45-0201 and BS4 45-0210)
BS4 45-0164
667.00 516 KIT
Includes Bent, L-shaped 1 mm
Electrode Gold Tip (BS4 45-0117),
Holder (BS4 45-0203), and Cables
(BS4 45-0201 and BS4 45-0210)
In Vivo Applications: Model 508 and Model 510
The Model 508 Genetrode with a sharp 5 mm electrode tip is a general
in vivo electrode. The Model 510 is the same electrode but with a 10 mm
tip. Similar electrodes have been used for a variety of applications.
Salford used an electrode of this style with a 600 Series to deliver
bleomycin into rat gliomas, doubling animal survival time.1 Yamaguchi
used a similar electrode with an 800 Series to deliver bleomycin into
subcutaneously grafted mouse bladder carcinoma, resulting in complete
regressions if the tumorous tissue.2 Nishi used this type of electrode with
a BTX Generator to deliver a plasmid DNA construct into rat gliomas,
resulting in expression of beta galactosidase in the area between the
electrodes.3 Muramatsu used similar electrodes with a BTX Generator in
his studies of LIVGET techniques in mouse testis, concluding that electroporation is superior to microparticle bombardment.4 Aihara and
Miyazaki used these electrodes with an 800 Series to transfer genes into
murine muscle tissue, significantly increasing expression levels over
DNA injection alone.5
Product
In Ovo Applications: Model 512, 514, and 516
References
The Model 512, 514, and 516 electrodes are all suitable for in ovo gene
transfer studies. Funahasi has used an electrode similar to Model 516
with an 800 Series for small-area gene transfer in ovo.6 An electrode similar to Model 514 has been used with a BTX generator by Muramatsu in
his in ovo studies.7 He concludes that electroporation is superior to both
lipofection and microparticle bombardment.8
1.)
Salford et al., Biochemical and Biophysical Research Communications, 194 (2):
938-943 (1993)
2.)
Yamaguchi et al., J. Exp. Med., 172: 291-293 (1994)
3.)
Nishi et al., Cancer Research, 56: 1050-1055 (1996)
4.)
Muramatsu et al., Biochemical and Biophysical Research Communications, 233:
45-49 (1997)
5.)
Aihara and Miyazaki, Nature Biotechnology, 16: 867-870 (1998)
7.)
Muramatsu, Mizutani, and Okumura, Anim. Sci.Technol. (Jpn.) 67 (10): 906-909 (1996)
8.)
Muramatsu et al., Biochemical and BioPhysical Communications, 230: 376-380
(1997)
Note: All Genetrode models require Genetrode Holder (BS4 45-0203), and Cables (BS4
45-0201 and BS4 45-0210)
M62
11,000 Specialty Products to Enhance Your Bioresearch
Electroporation Electrodes
NEW
Tweezertrodes™
Specifications
The BTX Tweezertrodes are reusable, tweezer style in vivo electrodes
for drug or gene delivery in animals. Tweezertrodes consist of a standard 11.5 cm tweezer that has been modified with stainless steel circular or disk electrodes at the tip. The gap between the electrode
disks may be adjusted from under 1 mm to over 2 cm. Tweezertrodes
come in two models. Model 520 has an electrode disk diameter of 7
mm and Model 522 has a diameter of 10 mm. Tweezertrodes may be
cleaned with a mild detergent and sterilized with ethanol or ethylene
oxide. These electrodes are connected to a pulse generator with the
Model 524 Connection Cable, and are compatible with most BTX
Electroporation Systems.
Voltage Range
0 to 1.0 kV
Pulse Length Range
10 µsec to 99 msec in PBS
Model 520:
Tweezertrode Length
Electrode Diameter
Electrode Material
11.5 cm (14.5 in)
7 mm
Stainless steel
Model 522:
Tweezertrode Length
Electrode Diameter
Electrode Material
11.5 cm (4.5 in)
10 mm
Stainless steel
Generator Compatibility
ECM T 820, 630, 830, 2001
Catalog No.
$
Model
Product
BS4 45-0118
663.00 520
Tweezertrode Electrode 7 mm diameter
BS4 45-0119
663.00 522
Tweezertrode Electrode 10 mm
diameter
90.00 524
Tweezertrode Connection Cable
BS4 45-0204
BS4 45-0165
714.00 520 KIT
Includes Tweezertrode 7 mm (BS4
45-0118) and Cable (BS4 45-0204)
BS4 45-0166
714.00 522 KIT
Includes Tweezertrode 10 mm (BS4
45-0119) and Cable (BS4 45-0204)
Note: All Tweezertrode models require Connection Cable BS4 45-0204
Cell Biology
Applications
Tweezertrodes may be used for many in vivo applications, including
gene and drug delivery. Following localized or systemic injection of
the molecule of interest, the Tweezertrode electrode disks are used
to grasp the tissue of interest. An electroporation pulse is then given,
initiating pore formation and incorporation of the molecule into the
cells of the tissue in direct contact with the electrode disks.
Suzuki et al. used Tweezertrodes to deliver a Green Fluorescent
Protein (GFP) gene into rat liver. The authors demonstrated successful gene transfer and expression in situ.1,2
Tweezertrodes complement BTX Models 508, 510, Caliper Electrode
Models 384 and 384L, 2-Needle Array Models 530 and 532 as the
newest addition to our in vivo electrode line. The Tweezertrodes can
be used to reproduce applications requiring the caliper electrodes,
including gene therapy, transdermal drug delivery and electroporation therapy.3,4,5,6
References
1.)
Suzuki et al., Direct Gene Transfer into Rat Liver Cells by in vivo Electroporation, FEBS
Letters, 425: 436-440 (1998)
2.)
BTX Electroporation Protocol PR0363 (1998)
3.)
Zhang, et al., In vivo transdermal delivery of large molecules by pressure-mediated electroincorporation and electroporation: a novel method for drug and gene delivery,
Bioelectrochemistry and Bioenergetics, 42: 283-292 (1997)
4.)
Dev, Giordano and Brown, In vivo delivery of gene to rabbit carotid artery by electroporation, Third U.S./Japan Symposium on Drug Delivery Systems (Abstr.), 38 (1995)
5.)
Dev, Killing cancer cells with a combination of pulsed electric fields and chemotherapeutic agents, Cancer Watch, 3: 12-14 (1994)
6.)
BTX Electroporation Therapy Protocols ECT 001 - ECT 005 (1995)
Call for your local BTX Dealer
or visit www.btxonline.com.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M63
Electroporation Electrodes
NEW
2–Needle Array™ Electrode
To Use:
While grasping the Needle Array handle, simply position the handle over
a needle array assembly and push to secure the needle array onto the
handle. Attach the handle to a BTX pulse generator via the high voltage
banana cables. Remove the needle safety shield, place into the tissue,
and deliver the pulse. Discard the needle array and prepare for the next
experiment.
Specifications
Voltage Range
0 to 500 V
Pulse Length Range
1 µsec to 99 msec in PBS
Specialized Tools For Bioresearch
Electrode Handles
The BTX 2-Needle Array Electrode is an in vivo style electrode specifically designed for muscle gene delivery. The electrode consists of a
reusable delrin 2-Needle Array Handle and sterile disposable two-needle array assemblies. The needle array assemblies are conveniently
packaged in “Six Packs,” allowing for guaranteed sterility and designed
to fit conveniently in a sterile hood. The needles themselves are made
of medical grade stainless steel.
The 2-Needle Array comes in 5 mm and 10 mm electrode spacings. The
Model 530 Handle with the Model 531 10 mm gap 2-needle array
assembly are recommended for larger muscle masses, such as rat gastronemus. The Model 532 Handle with Model 533 5 mm gap 2-needle
array assembly are recommended for smaller muscle masses, such as
mouse tibialis. Other species as well as other tissues may be electroporated with the 2-Needle Array.
Applications
Model 532
Handle Length
8 cm (3.2 in)
Handle Material
Delrin
Electrode Diameter 5 mm
Two-Needle Array Assemblies
Model 531:
Needle Length
Needle Material
Needle Diameter
20 mm
Stainless Steel
10 mm
Model 533:
Needle Length
Needle Material
Needle Diameter
20 mm
Stainless Steel
5 mm
Generator Compatibility ECM 830, 630 and 399
Muscle In vivo Gene Delivery (IVGD)
Among the non-viral techniques for gene transfer in vivo, the direct
injection of plasmid DNA into muscle is simple, inexpensive, and safe.
Aihara and Miyazaki (1998)1 showed that by combining electroporation with DNA injection, expression was enhanced 100 fold. Their
results demonstrated that gene transfer into muscle by electroporation
in vivo is more efficient than intramuscular DNA injection alone. Mir et
al. (1999)2 used various types of electrodes to deliver genes into skeletal muscles of various species (rat, mice, rabbits, monkeys), resulting
in 2- to 4-log fold increase in expression. DNA vaccination by direct in
vivo administration of plasmid-based DNA vectors has proven to be very
effective in animal models. Electroporation enhances gene expression
in muscle greatly, making it possible to induce immune response in
large animals without using large amounts of DNA. Therefore, in vivo
electroporation is proven to be especially useful at increasing effectiveness of DNA vaccinations in large animals.3
Other Applications
Electrodes similar to the 2-Needle Array have been used for a variety of
applications. Salford used an electrode of this style with a 600 Series to
deliver bleomycin into rat gliomas, doubling animal survival time.4
Yamaguchi used a similar electrode with an 800 Series to deliver
bleomycin into subcutaneously grafted mouse bladder carcinoma,
resulting in complete regressions of the tumorous tissue.5 Nishi used this
type of electrode with an 800 Series to deliver a plasmid DNA construct
into rat gliomas, resulting in expression of beta galactosidase in the area
between the electrodes.6 Muramatsu used similar electrodes with an 800
Series in his studies of LIVGET techniques in mouse testis, concluding
that electroporation is superior to microparticle bombardment.7
M64
Model 530:
Handle Length
8 cm (3.2 in)
Handle Material
Delrin
Electrode Diameter 10 mm
Catalog No.
$
Model
Product
BS4 45-0205
132.00 530
2-Needle Array Handle 10 mm
(requires BS4 45-0120)
BS4 45-0120
132.00 531
2-Needle Array 10 mm, pkg. of 6
(requires BS4 45-0205)
BS4 45-0206
132.00 532
2-Needle Array Handle 5 mm
(requires BS4 45-0121)
BS4 45-0121
132.00 533
2-Needle Array 5 mm, pkg. of 6
(requires BS4 45-0206)
BS4 45-0167
263.00 530 KIT
Includes 2-Needle Array 10 mm
(BS4 45-0120) and Handle 10 mm
(BS4 45-0205)
BS4 45-0168
263.00 532 KIT
2-Needle Array 5 mm (BS4 45-0121)
and Handle 5 mm (BS4 45-0206)
References
1.)
Aihara and Miyazaki, Nature Biotechnology, 16: 867-870 (1998)
2.)
Mir et al., PNAS, 96-4262-4267 (1999)
3.)
Personal Communication, Dr. Georg Widera, Genetronics, Inc. (1999)
4.)
Salford et al., Biochemical and Biophysical Research Communications, 194 (2):
938-943 (1993)
5.)
Yamaguchi et al., J. Exp. Med., 172:291-293 (1994)
6.)
7.)
Nishi et al., Cancer Research, 56: 1050-1055 (1996)
Muramatsu et al., Biochemical and Biophysical Research Communications, 233:
45-49 (1997)
11,000 Specialty Products to Enhance Your Bioresearch
Electroporation Electrodes
NEW
Genepaddles™
Specifications
The BTX Genepaddles are paddle-style reusable electrodes suitable
for a variety of applications. Genepaddles come in two models, each
model consisting of a pair of electrodes. The Model 542
Genepaddles feature rectangular 3 x 5 mm paddles, while the Model
543 Genepaddles feature rectangular 5 x 7 mm paddles. The models
vary to suit the size of the target electroporation area. These
electrodes are connected to a Model 645 Square-post Cable and
interfaced with a pulse generator via a Model 5343 Banana-Coaxial
Cable. The Genepaddles may be positioned in parallel at a
predetermined gap within a tissue using the Model 515 Genetrode
Holder. Genepaddles may be cleaned with a mild detergent and
sterilized with ethanol or ethylene oxide. Properly maintained
Genepaddles have a life span of approximately 200+ sets of pulses
and are compatible with most BTX Electroporation Systems.
Voltage Range
0 to 200 V DC (Do not use AC current)
Pulse Length Range
1 to 99 msec in PBS
Pulse Number Range
1 to 99 (depending on voltage and pulse
length settings)
Model 542:
Paddle Dimensions
Paddle Configuration
Paddle Thickness
Gold Plating Thickness
3 x 5 mm
Rectangular
1 mm
0.04 mm
Model 543:
Paddle Dimensions
Paddle Configuration
Paddle Thickness
Gold Plating Thickness
3 x 7 mm
Rectangular
1 mm
0.04 mm
Generator Compatibility
ECM® 830, 2001 and 630
Genetrode Holder Inter-Electrode Gaps 1 to 10 mm in 1 mm increments
All Genepaddle electrodes are gold plated, noninvasive electrodes.
Catalog No.
$
Model
291.00 542
Genepaddles 3 x 5 mm
BS4 45-0123
291.00 543
Genepaddles 3 x 7 mm
BS4 45-0203
265.00 515
Genetrode Holder
BS4 45-0201
50.00 465
Square-Post Cable
BS4 45-0210
97.00 5343
Banana-Coaxial Connection Cable
703.00 542 KIT
Includes Genepaddle 3 x 5 mm
(BS4 45-0122), Holder
(BS4 45-0203), and Cables
(BS4 45-0201 and BS4 45-0210)
BS4 45-0170
703.00 543 KIT
Includes Genepaddle 3 x 7 mm
(BS4 45-0123), Holder
(BS4 45-0203), and Cables
(BS4 45-0201 and BS4 45-0210)
In Vitro Embryo Gene Delivery
Note: All Genepaddle models require Genetrode Holder (BS4 45-0203), and Cables
(BS4 45-0201 and BS4 45-0210)
Cell Biology
BS4 45-0169
Applications
Genepaddles were designed for In Vitro Embryo Gene Delivery
(IVEGD) in mouse embryo. The electrodes are placed anterior and
posterior to the embryo following injection of the molecule of interest, and an electroporation pulse is delivered using a BTX
Electroporation System, such as the ECM 830, 2001, and 630.
The electric field introduced by the Genepaddles causes transient
pores to form in the cells of the tissue, allowing uptake of the
molecules into the cells. Itasaki1 used a 3 x 5 mm Genepaddle-style
electrode with a BTX Generator to deliver DNA to mouse embryo in
vitro. His success demonstrates the potential of electroporation to
transfect different types of tissues or embryos besides the classical
avian embryo.
Product
BS4 45-0122
In Vivio Gene Delivery
Paddle-style electrodes have also been used for in vivo applications.
Miyasaka2 used an electrode of this style with a BTX Generator to
deliver DNA in vivo into the cerebral corical anlage of E15 rat
embryos. He demonstrated high-efficiency gene transfer into intact
neural tissue using electroporation. Electroporation has the benefits
of being less labor-intensive and more economical than competitive
methods for in vivo gene transfer, such as viral methods and biolistics. Electroporation also produces greater viability and efficiency
than competitive methods for in vivo gene transfer.
References
1. )
Itasaki et al., ‘Shocking’ developments in chick embryology: electroporation and in
ovo gene expression , Nature Cell Biology, 1: E203-E207 (1999)
2. )
Miyasaka et al., Foreign gene expression in an organotypic culture of corical
anlage after in vivo electroporation, NeuroReport 10: 2319-2323 (1999)
Call for your local BTX Dealer
or visit www.btxonline.com.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M65
Electroporation Electrodes
Specialized Tools For Bioresearch
NEW
Caliper Electrodes
Specifications Sheet
The BTX Caliper Electrodes are
in vivo style re-usable electrodes
that are used for a variety of
applications. Caliper Electrodes
come in two models, with each
model consisting of a caliper
and a pair of end plates. Model
384 are used for smaller animals with a smaller surface
area, while model 384L is used
to target slightly larger surface areas. The end plates of the Caliper Electrode may
be adjusted by using the black roller mounted on the caliper. Caliper Electrodes
may be cleaned with a mild detergent and sterilized with ethanol or ethylene
oxide. Properly maintained Caliper Electrodes have an unlimited life, and are
compatible with most BTX Electroporation Systems.
Applications
Caliper Electrodes may be used for many electroporation applications, including
drug and gene delivery. The electrodes span the target area following injection of
the molecule of interest, and electroporation pulses are delivered using a BTX
Electroporation System, such as the ECM 630 or ECM 830. The electric field
introduced by the Caliper Electrodes cause transient pores to form in the cells of
the tissue, allowing uptake of the molecules into the cells. Zhang et al. used the
Caliper Electrodes to deliver molecules to skin for gene therapy studies on skin
aging.1 Mir et al. used the Caliper Electrode for in vivo skeletal muscle work2 as
well as subcutaneous tumor electrochemotherapy applications.3 Caliper
Electrodes can potentially be used for intact plant work as well.4
Caliper Electrodes complement BTX Tweezertrodes Model 520, 522; BTX
Genetrodes Model 508(S) and 510(S); and BTX 2 Needle Array Electrodes
Model 530, 531, 532, 533 as part of the BTX in vivo electrode line. Caliper
Electrodes can be used to reproduce applications requiring tweezertrodes
(transdermal) and Genetrodes/2-Needle Array electrodes (in vivo muscle).
This product meets the requirements of the European Communities (EC), and
complies with EMC Directive 89/336/EEC and 73/23/EEC for product safety.
NEW
Petri Dish Electrode
The Petri Dish Electrode is designed to be used with a
100 mm petri dish which functions as the
electroporation chamber. To perform electroporation or
electrofusion, the samples are placed in the petri dish
and the electrode assembly is lowered into the dish.
The electrode assembly is available in a 2 mm gap size.
It contains parallel stainless steel electrodes which
produce homogeneous fields. The electrodes are
designed to electroporate volumes ranging from 4 to 50
ml. The Petri Dish Electrode can be used with all BTX
electroporation or electrofusion systems.
Applications
The 2 mm gap Petri Dish Electrode can be used for
electroporation of tissues grown right in the petri dish.
Simply add the exogenous molecule of interest, mix
and electroporate.
Catalog No.
BS4 45-0100
$
Model
1,275.00 366
Product
100 mm Petri Dish
Electrode, 2 mm gap
50 ml
Specifications
Voltage Range
0 to 500 V (depending on electrode gap)
Pulse Length Range
1 µsec to 99 msec (multiple pulsing permitted)
Model 384:
Electrode Dimensions
Electrode Material
1.0 x 1.0 cm
brass
Model 384L:
Electrode Dimensions
Electrode Material
1.5 cm x 1.5 cm (2.0 cm x 2.0 cm spare pair included)
stainless steel
Generator Compatibility
ECM 830, 600, 630, T 820
Electrode gap
0.1 to 13 cm
Catalog No
$
Model
Product
BS4 45-0101
484.50 384
1.0 x 1.0 cm brass end plates
BS4 45-0102
484.50 384L
1.5 x 1.5 cm stainless steel end plates
References
M66
1.)
Zhang, Lei et al., Biochemical and Biophysical Research Communications, 220, 633-635 (1996)
2.)
Mir et al., PNAS, 96, 4262-4267 (1999)
3.)
Mir et al., British Journal of Cancer, 77(12), 2336-2342 (1998)
4.)
Dev et. al, U.S. Patent 5,859,327 Jan 12, 1999
Call for your local BTX Dealer
or visit www.btxonline.com.
11,000 Specialty Products to Enhance Your Bioresearch
Electroporation Electrodes
NEW
Petri Pulser™
NEW
Microslides
The Petri Pulser PP35-2P is a reusable
electroporation applicator designed to fit into
each single well of a 6-well plate or an individual 35 mm diameter petri dish. The PP35-2P
consists of an electrode assembly embedded
in a polyurethane holder with high voltage
electrode cables. The thin electrodes are gold
plated and designed to maximize the surface
area of electroporation. The high voltage
cables are connected with a BTX Pulse
Generator, and the electrode head inserted
into the dish or well containing the sample. An
electroporation pulse may then be delivered. The entire applicator may be cleaned
with mild detergent, and the electrodes may be sterilized with ethanol and dried
with acetone.
™
Applications
The Petri pulser PP35-2P is designed for the electroporation of adherent cells in situ
or as an alternative to cuvette electroporation for larger volumes or multiple cell
samples. The use of the electrode for the electroporation of adherent cells in situ
eliminates the need for trypsinization or mechanical removal of cells from their
growth substrate. This eliminates associated problems including low plating
efficiencies following electroporation, interruption of cell cycle and interruption of
intercellular communication.
Mammalian Cell Protein/Drug Electroincorporation
The Petri Pulser PP35-2P supplements our Model 366 Petri Dish Electrode designed
for 100 mm petri dishes, as the ideal electrode for in situ electroincorporation of
protein into mammalian cells.1,2,3 This electrode has been used for electroincorporation of peptides and proteins in CHO cells4 and vascular smooth muscle cells.5
Plant,Yeast and Bacterial Electroporation Applications
Applications involving plant, yeast and bacterial electroporation may be carried out
using the Petri Pulser. Scale up and multiple sample processing.
This product meets the requirements of the European Communities (EC), and complies with EMC Directive 89/336/EEC and 73/23/EEC for product safety.
Specifications
Voltage Range
0 to 300 V
Electrode Thickness
0.5 mm
Pulse Length Range
1 to 35 msec in PBS
Electrode Material
Gold
Volume Range
0.5 to 3.0 ml
Generator Compatibility ECM 830, 630, 399
Weight
6 oz
Gap Size
Number of Electrodes
13
Catalog No.
BS4 45-0130
$
Model
489.00 PP35-2P
2 mm
Product
1.)
Marrero et al., Journal of Biological Chemistry, 270 (26): 15734-15738 (1995)
2.)
Scheiffer et al., Journal of Biological Chemistry, 271 (17): 10329-10333 (1996)
3.)
Marrero et al., Journal of Biological Chemistry, 272 (39): 24684-24690 (1997)
4.)
Fukai, Masuko, Emory University, Personal Communication (1998)
5.)
Fukai, Masuko, Emory University, Personal Communication (1998)
7.)
Catalog No.
$
Model Product
BS4 45-0103
306.00
450
Microslide 20 µl, pkg. of
10, 0.5 mm gap
BS4 45-0104
306.00
450-1
Microslide 40 µl, pkg. of
10, 1.0 mm gap
BS4 45-0105
128.00
453
Microslide 3.2 mm gap,
pkg. of 1
BS4 45-0106
128.00
453-10
Microslide 10 mm gap,
pkg. of 1
BS4 45-0210
97.00
5343
Coaxial Banana Plug
Connection Cable, 10 ft.
BS4 45-0200
50.00
464
Micrograbber Cables, for
use with Microslide BS4
45-0103 and BS4 45-0104
BS4 45-0201
50.00
465
Square Post Cables, for
use with Microslide BS4
45-0105 and BS4 45-0106
Petri Pulser Electrode
References
6.)
Applications
The Microslides have been used for embryo manipulation1 and cell or protoplast fusion.2 Advantage of the
Microslide is that the process of dimmer formation can
be observed under the microscope.
BTX Protocols PR0165 and PRO330 (1998)
BTX Protocols PR0290, PR00286, PR0359, PR0360, PR0364 (1998)
Cell Biology
Mammalian Cell Transfections/Gene Therapy
The Petri Pulser PP35-2P is capable of supporting transfection protocols developed
for our larger Model 366 Petri Dish Electrode, including portocols for BHK and
HUVEC.6 The electrode itself has been used for the transfection of 3T3-L1, rat
adipose cells, SF295 human neuroglioblastoma and Cos 7 cells.7
The BTX Microslides are used primarily with the ECM®
2001 Electro Cell Manipulator. The Microslide fits on the
microscope allowing observation of the alignment and
fusion process. The Microslide 450 is composed of a
glass microslide and two strips of 0.5 mm stainless steel
tubing which functions as electrodes to provide a divergent field. These wires are set apart at different gaps to
provide various field strengths and volumes. The
Micrograbber is used to connect the Microslide 450 to
the Coaxial Connection Cable, which will then be hooked
up to the ECM 2001 generator. Microslide 453 has two
stainless steel square bars that are set apart at various
gaps to provide homogeneous fields. The Square Post
Cable is used to connect the Microslide to the Coaxial
Connection Cable which will be hooked up to the ECM
2001 generator.
References
1.)
BTX Protocol PR0117, Personal communication with Dr. James
Robl, University of Massachusetts.
2.)
Saunders, J.A. and Bates, G.W., Guide to Electroporation and
Electrofusion, Academic Press, p. 477-479, 1991
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M67
Electroporation Electrodes
Specialized Tools For Bioresearch
NEW
Meander Fusion
Chamber
The BTX Meander Fusion Chamber is a
microslide with a novel design specifically
used for electro cell fusion. This chamber is
constructed of a conductive metal alloy
which has been deposited in a finger-like
projection array on a glass microscope
slide. This method of manufacturing creates
a highly precise working area. The configuration is designed to give direct viewing of a
surface area. This can be used for viewing
dimmer formation during alignment while
under a microscope. The gaps are set at
0.2 mm.
Application
The Meander Fusion Chamber generates a
homogenous field and is used for cell fusion
of microorganisms, plant, fungi, yeast and
mammalian cells.
NEW
Flatpack
Chambers
Flatpack Chambers are constructed as a new
design, featuring a three-ply solid sandwich
construction of stainless steel and mylar
plastic. Flatpack Chambers are gamma sterilized in individual packages. They are provided in sets of 50 and may be used in the
Safety Stand along with the Flatpack Slider.
Applications
The Flatpack is used for prokaryotic applications. It is a unique flow-through design
with a 0.56 mm gap and a volume from 10
to 85 µl. This design provides the unique
combination of small sample volumes with
field strength as high as 40 kV/cm. Ease of
use and safety features required for the high
efficiency transformation of certain gram
positive organisms, such as E. coli1.
BS4 45-0107
BS4 45-0202
BS4 45-0210
$
Product
765.00 Meander Fusion
Chamber, product 454,
0.2 mm Gap on Glass
Microslide, pkg. of 4
(requires Cables
BS4 45-0202 and
BS4 45-0210)
84.00 Square-Post
Micrograbber Cables,
product 465
Flat Electrode
The Flat Electrode Chambers are made of
two rectangular, parallel plates of high grade
stainless steel that is press-fitted into a polysulfone base. They generate fields depending on the orientation of the grooved electrodes. Orientating the grooved sides of each
electrode so that they face one another generates a divergent field for use in electrofusion experiments. The flat sides of each electrode will generate a homogenous field
when facing one another. The Electrode
Adaptor is used to attach the Flat Electrode
Chamber to the BTX instrument.
Applications
This Flat Electrode Chamber can be used for
both electroporation and electrofusion.
Catalog No.
Catalog No.
Catalog No.
NEW
$
Product
BS4 45-0109
306.00 Flatpack Chambers,
product 485, 1.83 mm
Gap 1.5 ml, Sterile,
pkg. of 50
BS4 45-0110
306.00 Flatpack Chambers,
product 486, 0.56 mm
Gap 80 µl, Sterile, pkg.
of 50
BS4 45-0202
84.00 Electrode Adapter,
product 466, for use
with Model 484
Dr. Steven Mayfield, Scripps Clinic and Research
Foundation (Molecular Biology), La Jolla, Californi
97.00 Coaxial Connection
Cable, 10 ft,
product 461
Call for your local BTX Dealer
or visit www.btxonline.com.
M68
Product
882.00 Flat Electrode /
Divergent or
homogeneous field,
product 484, 1.0 mm
gap; 0.5 ml (requires
Electrode Adapter BS4
45-0202 and Cable
BS4 45-0210)
Reference
1.)
$
BS4 45-0108
11,000 Specialty Products to Enhance Your Bioresearch
Electroporation Accessories
NEW
Disposabale Electroporation Cuvettes
Plus™ and Safety Stand
Individually wrapped single use
cuvettes expedite experimentation
•
Sterile-packaged reduces
contamination to important samples
•
Transfer pipet allows quick and easy
removal of sample
•
Color coding for easy cuvette gap
identification: Grey 1mm, Blue
2mm,Yellow 4mm
•
Round sealing caps allow easy,
single-handed cap removal
•
Polished aluminum results in lower
arcing frequency
•
Medical grade polycarbonate is
highest quality available
•
Three sizes available to suit all
applications: 1 mm Bacteria, 2 mm
Bacteria, Plant, Mammalian, 4 mm
Plant, Mammalian
The BTX Cuvettes Plus are designed for use in
electroporation of bacteria, yeast, mammalian, and plant cells. Each sterilized
Cuvettes Plus package includes a disposable
cuvette and a bonus "pipette" which is used
for removal of the sample after electroporation. The cuvettes are molded with embedded
aluminum electrodes, washed prior to packaging and gamma irradiated for guaranteed
sterility. Three electrode gap sizes are available, 4 mm, 2 mm and 1 mm for high field
strengths up to 25.0 kV/cm. Round caps are
leak resistant and allow for quick and easy
one finger removal. BTX disposable Cuvettes
Plus require no maintenance. Simply discard
carefully after use. For research use only.
™
BTX Cuvettes Plus
Specifications
Catalog No.
BS4 45-0124
BS4 45-0125
BS4 45-0126
Model
610
620
640
Cap Color
Gray
Blue
Yellow
Gap
1.0 mm
2.0 mm
4.0 mm
Field Type
Homogeneous
Homogeneous
Homogeneous
Sample Visibility
Yes
Yes
Yes
Minimum Volume
20 µl
40 µl
80 µl
Maximum Volume
90 µl
400 µl
800 µl
$
130.00
130.00
130.00
Generator
Footswitch for
ECM® 830
The BTX 1250Fs Footswitch is designed to
allow hands-free operation of the ECM® 830
Generator. The footswitch functions
identical to the Start button on the front
panel of the instrument. Once all the parameters are dialed in, simply press and release
the footswitch or Start button to activate
pulse delivery or abort a pulse sequence.
The BTX Generator Footswitch is designed
for used with BTX ECM 830 Square Wave
Generator. Versions 1.08 and beyond have
software that supports the footswitch operation, so no further modification of the generator is required. If you have a version
prior to V1.08, please contact BTX Customer
Service and please have the instrument serial number (on the back panel) ready to
expedite the process.
Applications
The BTX Generator Footswitch is especially
useful when conducting in vivo gene delivery,
in ovo gene delivery, and nuclear
transfer/cloning applications. These types of
experiments often require the researcher to
use both hands for other manipulations, generating the need for footswitch activation.
Cell Biology
•
NEW
The BTX Generator Footswitch complies
with EC Safety Directive, EN61010-1:
1993+A2: 95 for product safety.
Specifications
Dimensions, H x W x D 2 x 4.5 x 3.5 in
Catalog No.
BS4 45-0207
BS4 45-0208
$
Model
428.00 630B
30.00 660
Product
Weight
< 2 lb
Cord Length
10 ft
Cuvette Safety Stand (adjustable gap, for use with disposable
Cuvettes)
Catalog No. $
Cuvette Rack
BS4 45-0211 393.00 1250S
Model Product
Footswitch for
ECM 830
Generator
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M69
Microinjection & Microperfusion
Specialized Tools For Bioresearch
Introduction to Microinjection
The use of Glass Needle (Fine Glass Microcapillary Pipette)
based techniques for intracellular/extracellular microinjection and
perfusion has become a popular procedure in numerous areas of experimental biology research (e.g. In vitro fertilization, transgenics, etc.).
These techniques can best be described as micro surgical procedures that
are conducted on a single cell using either a single or multiple barrel
glass micropipette a precision positioning device (micromanipulator)
and a microinjector or microperfusor. The micropipettes used in these
procedures are formed using a Pipette Puller. The capillary glass
tubing is heated to its softening point and ‘pulled’ to create the proper
size tip diameter and taper for the desired application. The small tip
diameters (as low as 0.2 µm) of these micropipettes combined with the
high precision of the Micromanipulator and Microinjection/
Perfusion Apparatus, allows for precise and accurate delivery. This
precision allows for accurate and repeatable injections down to the subpicoliter liter range into or around various types and sizes of cells with
accuracy to 0.1 micron. The process of extruding substances through
these micropipettes is accomplished through the use of either direct
hydrostatic pressure (Pressure Injection) or by moving charged
ions that are the result of an applied electric field (Iontophoresis)
without the use of hydraulic flow.
Warner and Harvard Apparatus Clark Capillary Glass, pages M45 to M49
Pipette Pullers, pages M51 and M52
Micromanipulators, pages M186 to M201
Applications of microinjection range from assisted (In vitro) cell fertilization techniques to the transport of molecular and cellular elements.
These substances are typically injected into the cell to manipulate
and/or monitor the fundamental biochemistry of a specific living cell.
Substance that can be injected include, cellular organelles, kinases, histochemical markers (such as horseradish peroxidase or lucifer yellow),
proteins, metabolites, microbeads, ions, antibodies, genes, molecular
biology mRNA and DNA, etc. The precise delivery (microperfusion) of
small volumes (picoliter to milliliter) of various agents and drugs to
a cell or group of cells for applications such as pharmacological drug
testing can also be accomplished using these techniques.
In order for the researcher to conduct any of the above experiments and
get meaningful results, the tools (equipment) used in these experiments
must provide not only the specific functionality, but they also need to
be of the highest quality and provide the necessary reliability, accuracy
and repeatability to insure proper results. Harvard Apparatus, Inc.
manufactures and sells the complete range of product that are needed
to successfully microinject and/or microperfuse.
Harvard Apparatus/
Medical Systems
Picoliter Injectors,
pages M71 and M72
Harvard/
Apparatus
Medical Systems
NeuroPhore,
pages L2 to L8
Pressure Injection Systems, pages M75 and M76
M70
11,000 Specialty Products to Enhance Your Bioresearch
Microinjection & Microperfusion
Harvard/Medical Systems PLI-100 Pico-Injector
Versatile
Deliver volumes from femtoliters to microliters with the same instrument, resulting in a wide range of applications.
Pressure Capabilities
The PLI-100 features two negative and three positive pressure pneumatic capabilities.
The negative, or vacuum functions allow the user to:
BS4 65-0001 PLI-100 Pico-Injector
•
5 pressures: inject,
balance, clear, fill and hold
•
Reliable
•
Femtoliter to microliters
•
Reproducible
•
Easy to use
The PLI-100 has become a favorite
of prestigious national microinjection workshops like Cold Spring
Harbor Laboratories and other
researchers worldwide. Other
companies have tried to design
similar systems, but the PLI-100 remains unparalleled in terms of ease of
use, durability, precision, and cost.
Researchers Say:
“The PLI-100 is very robust, in constant use in our lab.”
“Most importantly, the PLI-100 valves prevent cytosol and
yolk backflow into my pipette after oocyte injection.”
“It’s very easy to control.”
“The PLI-100 is heavily used on a daily basis, and works great.”
“I like the fact that I can use the PLI-100 with TTL pulses.”
“It works great in conjunction with my imaging system.”
“The balance pressure is a good option.”
“My injection pipettes rarely clog.”
Easy to Use
Inject with the touch of a button or a tap of the foot switch — it’s as
easy as that!
Reproducible
Pressure to the pipette is controlled precisely through a multi-turn
regulator, and is reported digitally for easy repeat. Injection time is
digitally controlled in 10 msec steps between 0.01 to 0.99 seconds
(and in 1 second steps between 1 to 99 seconds). Pipette tip diameter, and hence delivered volume, are easy determinations once the
timing and pressure are known.
1. Eject precise amounts of fluids.
2. Create a balance pressure which prevents backflow into the
micropipette following an injection.
3. Clear a micropipette of material in it.
Unique Features
The PLI-100’s important ‘Balance’, ‘Hold’ and ‘Clear’ functions are features not found on other microinjection units.
Balance
In addition to the ejection pressure, the Pico-Injector offers a secondary
balance pressure. This adjustable pressure keeps a positive pressure on
the injection pipette before and after injections. This eliminates dilution
caused by capillary action and aids in the prevention of clogging.
Wasteful continuous injection, which often occurs when no separate
balance pressure is offered by an injection device, is avoided.
Fill/Hold
There are two built-in vacuum generators to fill a micropipette from the
tip and to hold suspended cells. Filling the pipette from the tip is easier
than back filling. Suspended cells can be held with a second (holding)
pipette. The holding vacuum’s range accommodates most cell types.
Clear
Cell Biology
• Popular applications:
- Injection of mouse,
frog, zebrafish and
other oocytes
- Extracellular brain
injections
- Injection of DNA,
mRNA, microbeads,
neurotransmitters,
kinases and other
proteins
• Most Published Injector
The PLI-100 Pico-Injector reliably
delivers a wide range of volumes
through micropipettes by applying
a regulated pressure for a digitally
set period of time. Compressed gas
allows the user to deliver desired
volumes from femtoliters to microliters while simultaneously holding
a cell. Whether you need to do
large injections into capillaries or
very small injections into mammalian nuclei, the PLI-100 is well
suited for your experiment.
1. Fill micropipettes from their tips, reducing wastage of
valuable injectables.
2. Provide a means to secure and manipulate a cell using a
holding pipette.
The positive pressures allow the user to:
A high-pressure pulse can be used for clearing a pipette, should it happen to clog. This is particularly useful when working with pipettes sized
for smaller volumes.
Electrical Connectors
BNC type connectors are available at the front panel to ease integrating
the Pico-Injector with other equipment. Synchronization of injections to
other stimulations or recordings is therefore possible.
Selection Guidelines
The PLI-100 is available in three packages: Plus, Basic and Deluxe. The
basic unit is supplied with an input hose, output hose, holding hose,
power cord and instruction manual. The plus unit also includes a foot
switch, pipette holder and input hose adapter. The deluxe unit includes
all the previously mentioned accessories plus an additional foot switch
and one pipette holder.
For Clark Electromedical Capillary Glass,
see pages M47 to M49.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M71
Microinjection & Microperfusion
Harvard/Medical Systems
PLI-100 Pico-Injector
Harvard/Medical Systems PLI-90
Picoliter Injector
Specialized Tools For Bioresearch
Specifications
•
3 pressures — inject,
balance, and clearing
0.2 to 60 p.s.i. (413 kPa), regulated, multi-turn
control
•
Eliminates backflow into
pipette after injection
Balance Pressure
0.1 to 3.5 p.s.i. (68.9 kPa), regulated, multi-turn
control, other ranges available upon request
•
Fill Vacuum
Internally produced, -12.0 p.s.i. (-82 kPa), unregulated
Reproducibly delivers femtoliters to microliters
•
Easy to use
Holding Vacuum
Internally produced, 0 to 3 in H2O (0 to 0.75 kPa
or 0 to 0.1 p.s.i.), regulated
•
Economical
Clearing Pressure
Input gas pressure, unregulated
•
Precise
Input Gas Pressure
70 to 105 p.s.i. (480 to 720 kPa)
Injection Pressure
Injection Timer
0.01 to 0.99 sec in 10 msec steps; 1 to 99 sec in
1 sec steps
Pulse Width
Injection Count Display Digital, 0 through 9999
The PLI-90 Pico-Injector, like the reliable PLI-100 Pico-Injector, see following pages,
controls the precise and reproducible regulation of injection pressure and time.
Duration Mode
Internally timed or externally gated
Time Trigger
Front panel, foot switch, or external TTL pulse
(BNC)
Pressure Units
p.s.i./kPa; switch selectable
Pressure Monitor
BNC connector, 10 mV/p.s.i.
Pressure Readout
Inject, balance, clear, output port
Selection Guidelines
Line Voltage
100/110/220/240 VAC
Power Usage
220 W
Meter Accuracy
0.1% full scale
The PLI-90 is ideal for the user who does not require vacuum for
filling pipette barrels or if using a holding pipette for holding cells is not essential
to your application.
Foot Switches
Inject, fill, hold, and gated; provided in plus and
deluxe pkgs.
Weight
6.8 kg (15 lb)
Dimensions, H x W x D
11 x 38 x 25.5 cm (5 x 15 x 10 in)
Accessories Supplied
Input, output and holding hoses
Catalog No.
BS4 65-0001
BS4 65-0002
BS4 65-0003
M72
BS4 65-0004 PLI-90 Pico-Injector
$
Model
5,018.00 PLI-100
5,195.00 PLI-100 Plus
Simplicity
Because the PLI-90 features only the injection, balance, and clearing positive pressures, it is a lower cost alternative to the PLI-100. This simplicity makes it even easier to use than the PLI-100.
The PLI-90 is available in two packages: Basic and Plus. The basic includes an
input and output hose, handle, power cord and instruction manual. The plus
model is supplied with all of the same parts as the basic model but also includes a
Footswitch, Pipette Holder, and Input Hose Adapter
Product
Specifications
PLI-100 Basic Pico
Injector with Injection,
Balance, Clear, Filling
and Holding Pressures;
Comes with Input Hose,
Output Hose, Holding
Hose, Power Cord and
Instruction Manual
Input Gas Pressure
70 to 105 p.s.i. (480 to 720 kPa)
Injection Pressure
0.2 to 60 p.s.i. (413 kPa), regulated, multi-turn control
Balance Pressure
0.1 to 3.5 p.s.i. (68.9 kPa), regulated, multi-turn control,
other ranges available upon request
Clearing Pressure
Input gas pressure, unregulated
Injection Time
0.01 to 0.99 sec in 10 msec steps; 1 to 99 sec in 1 sec steps
Pressure Display
Digital, three and a half digits
Duration Mode
Internally timed or externally triggered
Trigger Mode
Foot or panel switch
Pressure Readout
Inject, balance, clear, output port
Line Voltage
100/110/220/240 VAC
Power Usage
220 W
Foot Switch(es)
Optional inject and gating
Accessories Supplied
Input hose, output hose and power cord
Weight
6.8 kg (15 lb)
Dimensions, H x W x D
11 x 38 x 25.5 x cm (5 x 15 x 10 in)
PLI-100 Plus Pico
Injector with Injection,
Balance, Clear, Filling
and Holding Pressures;
Comes with Input Hose,
Output Hose, Holding
Hose, Power Cord,
Instruction Manual, Foot
Switch, Pipette Holder
and Input Hose Adapter
5,351.00 PLI-100 Deluxe PLI-100 Deluxe PicoInjector with Injection,
Balance, Clear, Filling
and Holding Pressures,
Comes with Input Hose,
Output Hose, Holding
Hose, Power Cord,
Instruction Manual, Two
Foot Switches, Two
Pipette Holders and
Input Hose Adapter
Catalog No.
$
Model
Product
BS4 65-0004
3,849.00 PLI-90 BASIC
Pico-Injector with Injection, Balance and Clear
Pressures; Includes Input Hose (PLI-IHN) and
Output Hose (PLI-OHN), Handle, Power Cord
and Inustruction Manual. Applications: Basic
setups that do not need suction.
BS4 65-0005
4,067.00 PLI-90 PLUS
Pico-Injector Basic Model and Acces-sories
(Shown Above), Plus One Each PLI-FS Foot
Switch, PLI-PH1 Pipette Holder and PLI-IHA
Input Hose Adapter
11,000 Specialty Products to Enhance Your Bioresearch
Microinjection & Microperfusion
Harvard/Medical Systems PLI-100 and PLI-90 Accessories
Catalog No.
$
Model
Product
Accessories
Catalog No.
$
Model
BS4 65-0029
71.00 PLI-FS
Footswitch for Inject, Hold, Fill or
Gate (Order more than one if
frequent use of footswitch is needed
for more than one of these functions.)
BS4 65-0030
50.00 PLI-RM
Frame to Mount PLI-90 or PLI-100 in
19-in. Wide Instrument Rack
BS4 65-0013
191.00 PLI-PH1
Stainless Steel Pipette Holder, 130
mm L for 1 to 1.5 mm OD Glass
Pipettes
BS4 65-0014
196.00 PLI-PH1A
Stainless Steel Pipette Holder, 80 mm
L for 1 to 1.5 mm OD Glass Pipettes
BS4 65-0017
19.00 PLI-SRG
Silicone Rubber Gasket Replacement
for use with PLI-PH1 and PLI-PH1A,
10.2 cm (12 in)
BS4 65-0015
19.00 PLI-SRG1.5
Silicone Rubber Gasket Replacement
for use with 1.5 mm Glass Pipettes
Gas Input Hose (Tygon Tubing), 9 ft x
0.250 in (6.35 mm) x 0.125 in (3.18
mm) x 0.062 in (1.57 mm) (L x OD x
ID x Wall Thickness); Connects to
PLI-IHA and PLI 90/100 Gas Input,
Brass Screw Fitting Type - Old
BS4 65-0018
11.00 PLI-SSB
Stainless Steel Bushing Replacement
for use with PLI-PH1 and PLI-PH1A
Pipette Holders
BS4 65-0019
22.00 PLI-PC
Stainless Steel Pipette Cap
Replacement for use with PLI-PH1 and
PLI-PH1A Pipette Holders
Gas Input Hose (Tygon Tubing) 9 ft L x
0.250 in (6.35 mm) OD x 0.125 in
(3.18 mm) ID x 0.062 in (1.57 mm)
Wall Thickness; Connects to PLI-IHA
and PLI 90/100 Gas Input, Quick
Connect/Disconnect Type - New
BS4 65-0020
19.00 PLI-HN
Stainless Steel Hose Nut Replacement
for use with PLI-PH1 and PLI-PH1A
Pipette Holders
Hosing
BS4 65-0006
BS4 65-0007
BS4 65-0008
BS4 65-0009
28.00 PLI-IHA
53.00 PLI-IHO
53.00 PLI-IHN
53.00 PLI-OHO
Input Hose Adapter (1/4 in Male BS4T
Pipe Thread) Gas Bottle Regulator
Fitting; Connects with PLI-IHO/N
Output Hose (Tygon Tubing) 9 ft L x
0.071 in (1.80 mm) OD x 0.039 in
(1 mm) ID x 0.016 in (0.41 mm) Wall
Thickness; Connects to MSC Pipette
Holders, Brass Screw Fitting Type - Old
64.00 PLI-OHN
Output Hose (Tygon Tubing) 9 ft L x
0.071 in (1.80 mm) OD x 0.039 in
(1 mm) ID x 0.016 in (0.41 mm) Wall
Thickness; Connects to MSC Pipette
Holders and PLI 90/100 Gas Output,
Quick Connect/Disconnect Type - New
BS4 65-0011
64.00 PLI-HHO
Holding Hose with Cell Release Bulb;
9 ft L x 0.071 in (1.80 mm) OD x 0.039
in (1 mm) ID x 0.016 in (0.41 mm) Wall
Thickness; Connects to MSC Pipette
Holders and PLI-100 Hold Output,
Brass Screw Fitting Type - Old
89.00 PLI-HHN
Holding Hose with Cell Release Bulb;
9 ft L x 0.071 in (1.80 mm) OD x 0.039
in (1 mm) ID x 0.016 in (0.41 mm) Wall
Thickness. Connects to MSC Pipette
Holders and PLI-100 Hold Output,
Quick Connect/Disconnect Type - New
Harvard Apparatus now owns
Clark Electromedical.
See pages M45 to M49
for industry standard
capillary glass.
BS4 65-0021
121.00 PLI-PH-KIT
Pipette Holder Hardware Replacement
Kit for use with PLI-PH1 and PLI-PH1A
Pipette Holders, Includes 1 each PLISSB, PLI-PC, PLI-HN and PLI-SRG
BS4 65-0022
42.00 PLI-PHA
Stainless Steel Pipette Holder (PLI-PH
series) Adapter for Eppendorf ECET
FEMTOTIP
Optional “Easy Insert” Acrylic Pipette Holder
Catalog No.
$
Model
Cell Biology
BS4 65-0010
BS4 65-0012
Product
Pipette Holder/Adapter/Parts
Product
BS4 69-0867
121.00 AO16 1.0
Acrylic Pipette Holder for
1.0 mm Pipettes
BS4 69-0868
121.00 AO16 1.2
Acrylic Pipette Holder for
1.2 mm Pipettes
BS4 65-0023
121.00 PLI-PPH
Acrylic Pipette Holder for
1.5 mm Pipettes
BS4 69-0869
121.00 AO16 1.2
Acrylic Pipette Holder for
2 mm Pipettes
Harvard Apparatus now owns the
NaviCyte line of Ussing and Diffusion
Chambers. See pages M166 to M170
for complete details on those products
and many others.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M73
Microinjection & Microperfusion
NEW
ZonaDrill™
Use ZonaDrill™ for trouble free Cell Nuclear Transfer, ICSI and
stem cell removal. ZonaDrill™ can also be used in conjunction with
fluorescence.
Available with the Cronus software suite for cell/embryo analysis and
digital target generator.
Specialized Tools For Bioresearch
Specifications
Ultra Focused Laser Beam:
Solid State Diode Laser
Power Density at Focus
Laser Focus Diameter
Non-Mercury
1480 nm/300 mW
4.5 mW/cm
2 µm
Pilot Laser
635 nm Spot Targeting Solid State Diode Laser
Objective Lens
40X
Catalog No.
The New ZonaDrill is a class 1
Laser System that incorporates
• High transmission
optics for reduced laser
the latest in fiber optic technolopulse time
gy to provide the most compact
• Non-contact laser
laser system currently available.
The ZonaDrill™ laser system is
• Mercury free Zona removal
mounted via the epifluorescence
• Digital display
port or side port of all popular
• Easy to use and install
brands of inverted microscope.
It can also be used in conjunction with all brands of micromanipulators and microscopes.
™
$
Product
BS4 72-6475
18,384.00 ZonaDrill™ for Olympus IX50 / IX70
BS4 72-6476
18,384.00 ZonaDrill™ for Olympus IX51 / IX71 / IX81
BS4 72-6477
18,384.00 ZonaDrill™ for Nikon Eclipse TE 200/300
BS4 72-6478
18,384.00 ZonaDrill™ for Nikon Eclipse TE 2000
BS4 72-6479
18,384.00 ZonaDrill™ for Nikon Diaphot 200/300
BS4 72-6480
18,384.00 ZonaDrill™ for Zeiss Axiovert 200
BS4 72-6481
18,384.00 ZonaDrill™ for Zeiss Axiovert 100/135
BS4 72-6482
18,384.00 ZonaDrill™ for Leica DMIRB
Rapid Zona removal is possible using the non-contact laser.
Laser ablation is much faster than mechanical methods such as piezo
electric devices and manual micromanipulation. Improve your efficiency and effectiveness in the lab with this market leading product.
A visible pilot laser is used to target the main invisible ablation laser
and the digital display informs the user when the laser is primed and
ready to fire. The pulse duration of the laser is adjusted according to
the hole size required. The new digital display and compact hand
control are unique features of this Laser system allowing easy and
quick operation of the laser.
The latest ZonaDrill™ laser system now incorporates a new laser
objective which provides a very high quality, high resolution image. It
has also been designed for high transmission of the laser so that
shorter pulse times are needed to ablate the zona, resulting in
increased embryo viability.
The ZonaDrill™ is the safest laser on the market today and is 100%
accurate guaranteed. The Unique Safety Circles, based on independent medical research, gives the user a visual indication of the Safety
Zone for the protection of critical cells. This laser system features a
unique hand-held control, a pilot laser targeting system and a digitally controlled pulse time. Additionally, the ZonaDrill™ requires no special needles/pipettes and is portable between microscopes.
M74
11,000 Specialty Products to Enhance Your Bioresearch
Microinjection & Microperfusion
Multi-Channel Pressure Injection Systems
BS4 69-0153 8-Channel
Pressure Injection
System
•
•
•
•
•
Multiple function system
for micro-injection and
perfusion
Up to 8 injection/perfusion
outputs, one hold cell
output and one synchronized drain out channel
Programmable timers,
counters and step
sequences which can
be stored for reuse
Manual control or
automatic sequential
step cycle operation
Front panel display for
pressures and programming information
The PM-4 and PM-8 programmable 4 and 8-Channel Pressure
Injector systems are designed for
one to eight channel intracellular
injection and extracellular perfusion. These microinjection and
perfusion engines are especially
designed for pharmacological drug
testing, molecular biological DNA,
RNA transferring, intracytoplasmic
sperm injection and cell electrophysiological applications.
Besides programmable timers and counters, there are 16 programmable
and savable injection/perfusion sequences with 32 programmable steps in
each sequence. Each sequence can be repeated continuously or triggered
manually. The interval time between steps can be programmed for automatic sequential cycle or manual trigger. There are two foot switch connectors for sequential step trigger and injection trigger. A digital remote
port can be connected to an optional remote key pad or be controlled by
a computer. With exceptional versatility and extremely precise control,
either the PM-4 or 8 is an ideal multi-channel injection/perfusion engine.
Connection Diagram for Extracellular Micro-Perfusion and Intracellular Micro-Injection Systems
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
Cell Biology
Combining an advanced microcontroller with precision pneumatic components, these systems
can simultaneously control up to
eight injection micropipettes or eight perfusion vials and one cell holding pipette and one drain pipette. The four (PM-4) or eight (PM-8)
injection/perfusion output ports can be controlled separately or com-
bined together to perform actions such as injection, capillary action balancing, suction or clear up. The cell holding output port can produce
an adjusted gentle suction to hold a cell, eject a pressure to push a cell,
or completely clear the holding pipette. Another drain output port can
simultaneously produce an adjusted suction to drain and clear out liquid before the next channel micro-perfusion. The PM-4/8 can deliver
different quantity agents and drugs from picoliters to continuous perfusion. All output pressures and vacuums can be regulated and can be displayed real-time on the front panel display. Previous pressure readings
can also be recovered on the display for setting comparison.
M75
Microinjection & Microperfusion
Multi-Channel Pressure Injection Systems
Cat. No.
BS4 69-0152 4-Channel
Pressure Injection
System
$
Qty Model Product
BS4 69-0152 8,263.00 1
PM4
4-Channel Pressure Injection System*
BS4 69-0153 9,875.00 1
PM8
8-Channel Pressure Injection System*
BS4 69-0155
53.00
OP1
Output Tubing with Connector and
4.5 ml Vial
PM-KP
Remote Key Pad
RK-2
Rack Mounting Kit with Handles,
BS4 69-0152 Only
BS4 69-0158
145.00
BS4 69-0160
68.00
BS4 69-0165
259.00
HOLD1
Holder for Single-Barrel Pipette for
BS4 69-0152 and BS4 69-0153
BS4 69-0166
271.00
HOLD4
Holder for 4-Barrel Pipette for
BS4 69-0153 and BS4 69-0152
Specialized Tools For Bioresearch
Below are the components supplied with each of the Pressure Injection Systems.
BS4
BS4
Pressure Injection System
69-0152 69-0153
Cat. No.
Product
Qty.
BS4 69-0154
IP1 Input Tubing with Connector
1
BS4 69-0156
OP2 Output Tubing with Connector Only 6
10
BS4 69-0157
FSW Foot Switch
1
1
BS4 69-0164
PP-4 Pulled 4-Barrel Pipette
1
-
BS4 69-0166
HOLD4 Holder for 4-Barrel Pipette
1
-
BS4 69-0163
PP-7 Pulled 7-Barrel Pipette
-
1
Qty.
1
BS4 69-0167
HOLD7 Holder for 7-Barrel Pipette
-
1
-
Power Cord
1
1
-
User’s Manual
1
1
Specifications
Output Channels
Four (PM-4) or eight (PM-8) injection / perfusion outputs,
one synchronized drain out and one cell hold channel
Input Gas Pressure
30 to 100 p.s.i.
Clearing Pressure
Same as input pressure
Clear Hold Pressure
0 to 3 p.s.i. (regulated)
Injection Pressure
0.05 to 60 p.s.i. (regulated)
Balance Pressure
0.05 to 10 p.s.i. (regulated)
Fill Vacuum
0 to 24 in Hg (regulated)
Hold Vacuum
0 to 30 in of water (regulated)
Drain Vacuum
0 to 30 in of water (regulated)
Repeatability
±0.02 p.s.i.
Display Accuracy
±0.05 p.s.i. for pressure, ±0.2 in for vacuum (< 2 in H2O)
Timer Setting Range
10 msec to 327.67 sec (10 msec resolution for whole range)
Counter Setting Range 1 to 255
Savable Sequences
16 sequences
Programmable Steps
32 steps for each sequence
Remote Control Ports
DB9 connector for remote key pad, foot switch jack and BNC for
negative TTL trigger, jack connector for injection foot switch
Signal Output Ports
Eight BNCs for injection signal on output port 1 to 4
Power Consumption
100, 120 or 220 VAC, 35 W
BS4 69-0156 Output Tubing
with Connector for use with:
BS4 69-0152, see above
BS4 69-0153, see above
BS4 69-0150, see page M52
BS4 69-0151, see page M52
BS4 69-0154 Input Tubing
with Connector for use with:
BS4 69-0152, see above
BS4 69-0153, see above
BS4 69-0150, see page M52
BS4 69-0151, see page M52
BS4 69-0155 Output Tubing
with Connector and 4.5 ml
Vial for use with:
BS4 69-0152, see above
BS4 69-0153, see above
BS4 69-0150, see page M52
BS4 69-0151, see page M52
BS4 69-0157 Foot Switch
Actuation for use with:
BS4 69-0152, see above
BS4 69-0153, see above
BS4 69-0150, see page M52
BS4 69-0151, see page M52
BS4 69-0164 and BS4 690163 Pulled Pipettes for
use with:
BS4 69-0152, see above
BS4 69-0153, see above
BS4 69-0158 Remote Key
Pad to Control for use with:
BS4 69-0152, see above
BS4 69-0153, see above
BS4 69-0150, see page M52
BS4 69-0151, see page M52
BS4 69-0160 Rack Mounting
Kit with Handles for use with:
BS4 69-0152, see above
BS4 69-0153, see above
BS4 69-0150, see page M52
BS4 69-0151, see page M52
M76
11,000 Specialty Products to Enhance Your Bioresearch
Microinjection & Microperfusion
Nanoject II/Auto Nanoliter Injector
Micromanipulator and
Stand sold separately
Specifications
Operation
Automatic
Glass Properties
Softening point 780°C
Glass Dimensions
1.14 x 0.053 mm (0.045 x 0.021 in) (OD x ID)
Injection Speed:
Fast: 46 nl/sec; Slow: 23 nl/sec
Fill Speed:
Fast: 46 nl/sec; Slow: 23 nl/sec
Empty Speed
92 nl/sec
Volume Range
2.3 nl to 69.0 nl
Change Volume
Dip switches #1 to #4
Change Inject Speed
Dip switch #5
Weight
99.2 g (3.5 oz)
Catalog No.
$
Product
BS4 69-0130
1,201.00 Nanoject II Nanoliter Injector, 115 V, 60 Hz, US Plug
BS4 69-0131
1,228.00 Nanoject II Nanoliter Injector, 230 V, 50 Hz,
European Plug
BS4 69-0132
1,228.00 Nanoject II Nanoliter Injector, 230 V, 50 Hz, UK Plug
Replacement Parts
18.00 Replacement Glass, 88.9 x 1.14 x 0.053 mm
(3.5 x 0.045 x 0.021 in) (L x OD x ID), Vial of 100
BS4 69-0134
23.00 Replacement Glass, 177.8 x 1.14 x 0.053 mm
(7 x 0.045 x 0.021 in) (L x OD x ID), Vial of 100
BS4 69-0135
21.00 Flared Glass, 90 mm (3.5 in), Vial of 100
BS4 69-0136
11.00 Standard O-Ring
BS4 69-0137
25.00 Special Flared O-Ring
BS4 69-0138
6.00 Replacement Wire Plunger
BS4 69-0139
8.00 Needle, 30 ga x 51 mm (2 in)
Accessories
BS4 69-0140
64.00 Footswitch
BS4 69-0141
121.00 Support Base
BS4 69-0142
133.00 Universal Mounting Adapter, see below photo
Cell Biology
The Nanoject II is the latest devel• Lower volume (2.3 nl)
opment in the Nanoject family of
capability
microinjectors. One of the unique
• Positive displacement
features of the Nanoject II is the
technology
use of a higher torque motor than
• 2.3 to 69.0 nl range
in previous models. This new
of volumes
motor is smoother in operation
• Minimal vibration
resulting in substantially reduced
tip movement. A new collect con• New improved high
torque motor
figuration holds the micropipette
more securely thereby reducing
• Membrane key pad
air infiltration and oil leakage.
• Improved micropipette
The two-speed fill/inject mode
holder
will allow the user to fill and inject
• Two speed
injection/fill rates
at half speed, facilitating the use
of smaller micropipettes. The
• Auto ‘Home’ capability
handling of more viscous samples
will also be improved. All of the
above factors enhance the precise delivery of sample.
BS4 69-0133
Volumes for the Nanoject II range from 2.3 nl to 69 nl. Although
previous models of the Nanoject series are excellent for Xenopus
injections, the smaller tips and smaller injection volumes of the
Nanoject II allow users to perform injections into other specimens/
applications that previously could not be performed.
Positive displacement technology and the use of precision micropipettes,
eliminate the need for tedious calibration when the viscosity of the sample changes. Contaminants in the sample do not hinder or change the
injection volume as with some other injectors. An optional universal
mounting adapter provides easy mounting of the Nanoject II to a variety
of micromanipulators and stereotaxic frames.
For the PLI-90 and PLI-100 Pico-Injectors,
see pages M71 to M73.
For HBIO/Warner’s Oocyte Clamping Products,
see pages H20 to H26.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M77
Microinjection and Microperfusion
Specialized Tools For Bioresearch
NEW
M78
Micropipettes
•
Ideal for cell research
•
Compatible with all
micromanipulator systems
•
Available in five bend
angles
•
Gamma irradiated
sterilization
•
Disposable
These Micropipettes are ideal for stem cell research. The Chemical Drilling Micropipettes are specially
designed for use with PiezoDrills. These disposable Micropipettes are individually sterilized and available
in five bend angles. They are manufactured in an independently validated clean room to FED.STD.209E,
class 10,000. All processes are controlled to BSEN46002 and ISO 9002, resulting in ultra-clean and uniform micropipettes.
Each Micropipette is individually packed in a Twista-Pak™ that ensures easy, damage-free removal of the
micropipette from its packaging. Five Twista-Paks are then sealed into a blister-pack to ensure that the
micropipettes remain sterile and protected during freight.
Micropipettes, pkg. of 5
Bend Angle
ID
Cell Holding Pipettes
15 µm
30 µm
Cell Removal Pipettes
20 µm
25 µm
30 µm
35 µm
0°
$
BS4 72-6496
25.00
BS4 72-6506
25.00
BS4 72-7592
25.00
BS4 72-7597
25.00
BS4 72-7602
25.00
BS4 72-7607
25.00
20°
$
BS4 72-6497
BS4 72-6507
BS4 72-7593
BS4 72-7598
BS4 72-7603
BS4 72-7608
25°
$
BS4 72-6498
25.00
BS4 72-6508
25.00
BS4 72-7594
25.00
BS4 72-7599
25.00
BS4 72-7604
25.00
BS4 72-7609
25.00
30°
$
BS4 72-6499
BS4 72-6509
BS4 72-7595
BS4 72-7600
BS4 72-7605
BS4 72-7610
35°
$
BS4 72-6500
25.00
BS4 72-6510
25.00
BS4 72-7596
25.00
BS4 72-7601
25.00
BS4 72-7606
25.00
BS4 72-7611
25.00
Bend Angle
ID
Cell Injection Pipettes
5 µm
8 µm
Stem Cell Injection Pipettes
14-17 µm
18-21 µm
0°
$
BS4 72-7582
25.00
BS4 72-7587
25.00
BS4 72-6486
25.00
BS4 72-6511
25.00
BS4 72-6491
25.00
BS4 72-6501
25.00
20°
$
BS4 72-7583
BS4 72-7588
BS4 72-6487
BS4 72-6512
BS4 72-6492
BS4 72-6502
25°
$
BS4 72-7584
25.00
BS4 72-7589
25.00
BS4 72-6488
25.00
BS4 72-6513
25.00
BS4 72-6493
25.00
BS4 72-6503
25.00
30°
$
BS4 72-7585
BS4 72-7590
BS4 72-6489
BS4 72-6514
BS4 72-6494
BS4 72-6504
35°
$
BS4 72-7586
25.00
BS4 72-7591
25.00
BS4 72-6490
25.00
BS4 72-6515
25.00
BS4 72-6495
25.00
BS4 72-6505
25.00
22-25 µm
11,000 Specialty Products to Enhance Your Bioresearch
Chemical
Drilling Pipettes
10 µm
Microinjection & Microperfusion
Screw-Actuated Air Syringes
For air-assisted
micro-injection and
micro-aspiration
•
Uncomplicated and
easy to use
•
Accurate
•
Sensitive control
•
High suction/pressure
These air-assisted microinjection/micro-aspiration syringes
provide the user with sensitive
control and an extremely low
dead-air volume. The capacity of
the syringe is 10 ml. When
required, the syringes can be
used to generate high pressure
or suction.
The screw-actuated syringe is a
small compact unit incorporated into a heavy circular base. Its convenient dimensions enable it to be ergonomically positioned next to
the control lever of a micromanipulator to facilitate single-handed
operation of both instruments.
The syringe is connected by hard polyethylene tubing to a micropipette, which in turn, may be fitted to a micromanipulator or
positioning device. Suction/injection is obtained by turning the metal
colored actuator screw-control on the top of the syringe. The syringe
provides a solution to the problem of capillary action when working
with small micropipettes. A small balance (equilibrating) pressure can
be maintained to offset the effects of capillary action. A conveniently
situated release-button on-top of the screw-control is provided to
achieve equilibration.
Catalog No.
BS4 69-0105
$
Product
2,051.00 Screw-Actuated Air Syringe
•
Ideal for oil-filled
injection/aspiration
•
Easy to use
•
Hamilton syringe
incorporated
•
Accurate
•
Sensitive control
•
Low dead-air volume
•
High suction/pressure
•
Easy to exchange or
replace the syringe
This screw actuated syringe is a
micrometer-actuated syringe
mechanism mounted on a small
base. This syringe is for
oil/air-assisted micro-injections
and micro-aspirations. Its compact dimensions enable it to be
ergonomically positioned next to
the control lever of a micromanipulator to facilitate single-handed
operation of both instruments.
Suction/injection is achieved by
using the rotary-actuator with a
fine micrometer-thread, which
has a 15 mm movement range. A Hamilton 1 ml Gastight glass syringe
with a Luer-taper is incorporated. A Luer-taper hypodermic needle is
mounted on the taper of the Hamilton syringe. The syringe is
connected by hard polyethylene tubing to a micropipette, which in
turn, may be fitted to a micromanipulator or similar instrument. The
1 ml Hamilton syringe can be easily exchanged for any other Hamilton
1700 syringe series including the 1000, 500, 200, 100 and 50 µl sizes,
see Pumps Section A.
Catalog No.
BS4 69-0107
$
Cell Biology
•
Screw-Actuated Micrometer
Driven Hamilton Syringe
Product
881.00 Screw-Actuated Micrometer Driven
Hamilton Syringe
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M79
Micro-Incubation
Specialized Tools For Bioresearch
Heating of Imaging and Recording Chambers: Application Fundamentals
a temperature different than that currently being perfused.
Why Temperature Control?
Researchers have long understood the importance of temperature
regulation in the study of cellular function. For example, a Medline
search using the keywords "temperature" and "cell" produced over
18,000 references since 2003, most addressing the effect of temperature on the biophysical and metabolic function of cells. In addition,
the performance of imaging system optics are exquisitely sensitive to
temperature as revealed by a shifting focal plane as the temperature
of the objective is varied. Therefore, for the scientist studying the function of cells in a recording environment, the importance of tight thermal control cannot be overstated. A number of issues confront the
researcher attempting to maintain temperature control. These
include maintaining a constant and uniform bath temperature
under various flow rates and controlling for changes in the ambient
temperature of the environment. In addition, effects due to the addition of cold drugs or solution to the perfusion path and heat sinks due
to microscope optics and stage adapters must be addressed.
General Issues
Sources of Heat Flux
Heat flux pathways can be generally characterized as either conductive, convective, or radiative. Traditionally, the greatest effort is
directed towards controlling conductive pathways, followed by convective pathways. We define a conduction heat pathway as one
wherein heat energy is transmitted through a continuous intervening material. These include the microscope stage or objective, the
perfusion solution, the chamber platform and other physical elements of the working environment. Heat flux through convective
heat pathways are generally less significant but include evaporation
or condensation and heat transfer due to the movement of air
across the imaging chamber. Radiative pathways are, in comparison, easily controlled and will not be considered here.
Space and Time Considerations
in Uneven Heat Conduction
We first consider sources of uneven heat distribution in the spatial
regime. These include poor heat conduction across the chamber bath
or from the platform to the chamber. Poor heat conduction across
the bath is revealed as a temperature gradient from one location to
another within the bathing solution. A gradient of this type is usually induced by an immersion objective placed directly under the
sample. Heat flux through this pathway can be significant, resulting
in as much as a 10°C temperature change within 3-5 mm. Another,
less dramatic, temperature gradient can be generated in the bath
by restricting the application of heat to just the chamber platform.
Since the platform applies heat only along the edge of a chamber, a
temperature gradient will be established due to the heat capacity of
water coupled with flow of relatively cold solution into the chamber.
Uneven heating in the temporal regime can be generated by varying the rate cool perfusion solution is introduced into a heated platform, or by introducing drugs or solution into the perfusion path at
M80
These considerations will be addressed in greater detail below.
Outgassing
Another significant issue related to the application of heat to perfusion solutions is termed outgassing. Under standard conditions,
atmospheric gas in contact with a liquid solubilizes into solution
and tends towards an equilibrium determined in part by the gas’
partial pressure and the temperature of the liquid. Generally speaking, cold liquids are capable of solubilizing a greater volume of gas
than a warm liquid. Thus, as the temperature of the liquid increases, dissolved gasses come out of solution resulting in the appearance
of bubbles.
Heating Methods
A number of approaches are used by investigators to regulate the
temperature of a sample under study. These include warming the
chamber platform and/or microscope optics using resistive, Peltier,
or liquid based application devices, channeling warm air across the
imaging chamber and/or optical components, and regulating the
temperature of the perfusing solution. Techniques for regulating the
temperature of perfusing solutions include preheating the solution
reservoir and/or using inline solution heaters in the perfusion pathway. Each strategy has its strengths and weaknesses and are discussed below.
How Temperature is Sensed
In general, temperature is measured using a thermistor or similar
device and fed back into a temperature controller which adjusts
power to the heating element. When regulating temperature it is
important to account for the response time of the feedback loop since
this can cause the system to oscillate.
Control and Regulation
Warner Instruments temperature controllers utilize two thermistors
per channel to provide monitoring and feedback information to the
controller. One thermistor is placed within the heated element (e.g.,
chamber platform, in-line heater, etc.) to measure the temperature
of the heater block. Information from this thermistor is fed back to
the control mechanism which applies sufficient power to the heater to
maintain the controller set point.
The second thermistor is usually placed in the solution path close to
the sample under study and measures the temperature at the sample. Information from this thermistor is used by the researcher to
readjust the set point of the controller and to monitor the performance of the system.
This approach is very effective in providing tight thermal control
(± 0.1°C) of the heating element, and consequently the perfusion
solution, as long as the feedback loop is sufficiently optimized.
Warner Instrument temperature controllers are provided with user
selectable feedback response time constants allowing the instrument
to accommodate different operating conditions
11,000 Specialty Products to Enhance Your Bioresearch
Micro-Incubation
Methodology
In-Line Solution Heating
The simplest and most direct approach for the application of heat to
a sample is to preheat the perfusion solution immediately prior to
its delivery to the chamber. The warmed perfusate washes over the
sample and maintains a uniform temperature at the sample, even
with variable flow rates. This method works well as long as solution continues to flow through the heater into the chamber.
A number of considerations are important when using this
approach. First, it is important to minimize the distance between
the heater and chamber. If too much heat is lost en route, the user
can attempt to raise the heater controller set point above the boiling point of the solution. In addition, care must be taken to maintain flow rates such that the solution temperature reaches equilibrium prior to leaving the heater.
Chamber Platform Heating
Solution Reservoir Heating
Heating the solution reservoir is not generally considered a successful strategy for maintaining temperature in the bath since most, if
not all, of the heat energy is lost en route to the chamber. However, this
technique is important if outgassing of solutions is a problem. Since
the gas load of a solution is dependent on partial pressure and temperature, preheating the solution at atmospheric pressure before
delivery to the final heater will minimize the occurrence of bubbles
in the bath, even if the solution is allowed to cool en route. Therefore,
solution reservoir heating is best used in combination with other heating techniques as described below.
Microscope Objective Heating
While necessary, the use of immersion objectives in imaging presents a difficult problem for the researcher attempting to maintain
temperature at the sample. Since the objective is in direct contact
with the solution or chamber and is placed immediately above or
below the sample, it presents a significant conduction pathway
within the system.
Heated Enclosures
Another strategy currently in use is to encase the imaging chamber
and microscope optics within a heated enclosure. The main advantage of this approach is that both the chamber and objective are maintained at a stable and uniform temperature. Heat is usually applied
to the environment by the introduction of warmed air to the enclosure. Some disadvantages are slow responses to changes in the thermal set point, possible introduction of airborne contaminates from
the blower system, and the requirement for bulky equipment on or
about the imaging system.
Discussion
Each heating strategy discussed above has strengths and weaknesses making it an incomplete solution for issues encountered with
heating. However, most experimental difficulties can be solved by
using these strategies in combination.
For example, platform heating alone is not sufficient to stably heat
a perfusing solution, but is necessary if solution flow is to be interrupted for any reason. In addition, the most effective method of
heating a perfusion solution is via an in-line heater. Therefore, an
optimal combination for experiments where tight thermal control
is desired under variable, very slow, or interrupted flow conditions
would be a combination of in-line and platform heating.
If solutions are stored cold and used before reaching operating
temperature, or if the temperature is changed during an experiment, then outgassing at the sample can be a significant problem.
This is addressed by including reservoir preheating in the experimental setup. However, reservoir heating is unable to supply heated solutions to the chamber and is best used in combination with
an in-line solution heater.
Of course, all three approaches can be used in combination to provide efficient thermal regulation under virtually any condition.
Warner Instrument Corporation provides high quality single and
dual channel temperature controllers, as well as solution reservoir,
chamber platform, and in-line solution heaters. We invite you to
call our technical support staff and we will be glad to aid you in
determining the best application of these heating strategies to your
experimental setup.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
Cell Biology
Another strategy is to heat the chamber platform. This is usually
achieved by using a resistive element and this approach is provided
for in Warner chambers. A slightly more sophisticated tactic would be
to supply heat to the platform using either a Peltier or liquid based
heating element. Regardless of the method employed, this strategy is
best suited for conditions where the solution flow rate is extremely
slow or nonexistent.
A number of considerations are important when using this
approach. First, the perfusing solution receives heat energy only
after entering the chamber, thus generating a temperature gradient within the bath. Second, since heat energy is only applied to the
edges of the chamber by the platform, the rate of heat transfer to
the solution is very slow. However, this approach excels for conditions where solution flow is nonexistent, such as maintaining a
constant temperature while loading cells with indicator dyes.
Heated objective collars are commercially available and are
designed to minimize heat flux through the optics. However, many
researchers indicate that small variations in temperature as the
collar cycles off and on induces an oscillating shift in the focus
plane. In addition, many researchers express concerns about the
effects of deep cycling an objective between significantly different
temperatures between uses. Some investigators address this issue by
maintaining the objective at a constant temperature, even when
not in use.
M81
Micro-Incubation
The Series 40 Family of Imaging Chambers
Narrow Bath
Field
Stimulation
Specialized Tools For Bioresearch
Closed Bath
Mount coverslips in seconds
Low Profile
RC-40 Quick Change Imaging Chambers
•
O-ring seals permit quick
exchange of coverslips
•
Anodized aluminum
base ensures good
heat transfer
•
Open and closed bath
designs
•
Use standard # 1 or #1.5
thickness coverslips
•
Ideal for rapid screening
assays
Warner Instruments is pleased to
introduce a new family of chambers designed to meet the need
for rapid screening of cells
grown on coverslips. The innovative design uses O-rings to seal
the coverslip to the polycarbonate chamber. Therefore little or
no silicone grease is needed and
mechanical fasteners are not
required for coverslip mounting.
Chambers are available for use
with 15, 18, and 25 mm round
coverslips. Round, low profile chambers maximize the viewing area
and provide optimal access for electrodes. Slotted bath chambers
allow rapid solution exchange. A version which includes platinum
wires may be used to study cardiac myocytes and other applications
requiring field stimulation. The RC-40HP High Profile Chamber
allows for a deep bath.
The Series 40 chambers are designed to be compatible with the
Warner QE-1 Quick Exchange Platform and the DH-35i Culture Dish
Incubator.
The QE-1 Quick Exchange Platform
The Warner QE-1 Quick Exchange Platform is a versatile base for the
RC-40 Series chambers, as well as many popular 35 mm glass bottom dishes. Removable perfusion and suction tubes allow solution
exchange in any of the RC-40 Series open bath chambers or 35 mm
dishes. The platform may be heated using any of the Warner temperature controllers.
The QE-1 can be mounted on most microscopes by using the Warner
Series 20 stage adapters. See page M86 for more information on the
QE-1.
M82
11,000 Specialty Products to Enhance Your Bioresearch
Micro-Incubation
Series 40 Low Profile Chambers for 15, 18 and 25 mm Coverslips
BATH DIMENSIONS
RC-40LP
40 mm
RC-40LP
25 mm coverslip
Volume:
308 µl
19.7 mm
RC-41LP
RC-41LP
18 mm coverslip
Volume:
139 µl
RC-42LP
•
Low profile design
permits low entry angle
of electrodes
Excellent optical and
mechanical access to
cells and tissue
•
When used with QE-1
Quick Exchange
Platform, both
temperature and perfusion control are possible
The Series 40 family of chambers was designed for research
requiring fast exchange of coverslips. The chamber design
consists of two parts: a polycarbonate chamber and a 40 mm
diameter aluminum base. Orings are used to seal the coverslip to the polycarbonate chamber and to hold the chamber
securely in the aluminum base.
Open bath models are designed
for electrophysiological recording and/or optical imaging, and are
used in applications including patch clamp, oocyte clamp, and physiological measurements on cell cultures and tissue preparations. The
low profile design permits good electrode access to the bath.
The RC-40LP, 41LP, and 42LP Chambers use 25, 18, and 15 mm
round coverslips, respectively, in a round bath design to provide
maximum viewing area. The chambers work equally well with both
water and oil immersion objectives.
The RC-40LP, 41LP, and 42LP Chambers are supplied with one
package of #1.5 coverslips each (25, 18, and 15 mm round,
respectively) and spare O-rings.
13.3 mm
15 mm coverslip
RC-42LP
Volume:
87 µl
4.9 mm
10.5 mm
Volumes are for
1 mm solution height
Catalog No.
$
Model
Cell Biology
•
Series 40 Low Profile
Open Bath Imaging Chambers
Product
BS4 64-0367
250.00 RC-40LP
Quick Change Chamber 25 mm
Low Profile
BS4 64-0368
250.00 RC-41LP
Quick Change Chamber 18 mm
Low Profile
BS4 64-0369
250.00 RC-42LP
Quick Change Chamber 15 mm
Low Profile
Accessories/Replacement Parts
BS4 64-0375
595.00 QE-1
Quick Exchange Heated Base for
RC-40 Series
BS4 64-0703
12.00 CS15R
Coverslips 15 mm Dia. #1 – Box /100
BS4 64-0384
12.00 CS18R
Coverslips 18 mm Dia. #1 – Box /100
BS4 64-0705
14.00 CS25R
Coverslips 25 mm Dia. #1 – Box /100
BS4 64-0713
12.00 CS15R15
Coverslips 15 mm Dia. #1.5 – Box /100
BS4 64-0714
12.00 CS18R15
Coverslips 18 mm Dia. #1.5 – Box /100
BS4 64-0715
15.00 CS25R15
Coverslips 25 mm Dia. #1.5 – Box /100
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M83
Micro-Incubation
RC-40 Series Closed Bath and High Profile Chambers
for 25 mm Coverslips
BATH DIMENSIONS
RC-40HP
25 mm coverslip
Volume:
308 µl
RC-40HP
Specialized Tools For Bioresearch
19.7 mm
6.5 mm
RC-43C
•
O-ring seals permit quick
exchange of coverslips
•
Anodized aluminum
base ensures good heat
transfer
•
Open and closed bath
designs
•
Use standard #1 or #1.5
thickness coverslips
•
Ideal for rapid screening
assays
The RC-40HP uses 25 mm coverslips to form the chamber bottom. Tapered 6.5 mm interior
sides provide clearance for
pipettes, injectors, etc. and
allow for a greater bath depth
than the 40LP Series.
The RC-40HP Chamber is supplied with one package of #1.5
coverslips, 25 mm diameter.
The RC-43C is the closed bath
member of the Series 40 family.
The closed bath promotes an
even and continuous solution exchange and eliminates changes in
focus due to alterations in bath height. Studies involving physiological bicarbonate buffers can be performed with no gas loss at the
chamber interface.
Fluid is delivered to a 20 gauge stainless steel perfusion inlet port
and removed from the chamber by means of a similar outlet port.
The top coverslip is held in place with a retainer; a thin coating of
silicone grease forms the seal. The distance between top and bottom
coverslips is 1 mm.
The RC-43C Chamber is supplied with one package each of #1.5
coverslips (25 and 18 mm round, respectively) and spare O-rings.
Additionally, the RC-43C is supplied with 10 feet of PE-160 and PE90 Polyethylene Tubing.
M84
18 mm top
coverslip
RC-43C
Volume:
213 µl
25 mm bottom coverslip
15.8 mm
Volumes are for
1 mm solution
height
Catalog No.
6.3 mm
Input/Output Tubing PE-90
$
Model
Product
BS4 64-0370
250.00 RC-40HP
Quick Change Chamber High Profile
BS4 64-0371
280.00 RC-43C
Quick Change Chamber Closed Bath
Accessories/Replacement Parts
BS4 64-0375
595.00 QE-1
Quick Exchange Heated Base for
RC-40 Series
BS4 64-0384
12.00 CS18R
Coverslips 18 mm Dia. #1 – Box /100
BS4 64-0705
14.00 CS25R
Coverslips 25 mm Dia. #1 – Box /100
BS4 64-0714
12.00 CS18R15
Coverslips 18 mm Dia. #1.5
– Box /100
BS4 64-0715
15.00 CS25R15
Coverslips 25 mm Dia. #1.5
– Box /100
11,000 Specialty Products to Enhance Your Bioresearch
Micro-Incubation
Series 40 Slotted Bath Low Profile Chambers for 25 mm Coverslips
BATH DIMENSIONS
40 mm
All chambers
use 25 mm
coverslips
RC-46SNLP
Volume:
75 µl
4 mm
RC-46SNLP
18.9 mm
RC-46SLP
RC-47FSLP
Volume:
118 µl
6.5 mm
RC-46SLP
18.9 mm
Low profile design
permits low entry angle
of electrodes
•
Slot-shaped bath for
applications requiring
rapid perfusion
•
Platinum electrodes for
applications requiring
field stimulation
•
When used with
QE-1 Quick Exchange
Platform, both
temperature control and
perfusion are possible
The low volume of these slotted
bath chambers and the natural
laminar flow allows rapid
exchange of solution, facilitating
drug application studies.
The RC-47FSLP includes a pair of platinum wires placed onto the
parallel sides of the oval shaped bath. The connecting wires are terminated in 1 mm pins. This model includes a perfusion inlet port
and a 16 gauge adjustable suction tube. An agar bridge well has been
added to accommodate a bath ground electrode.
All chambers mount in the QE-1 Quick Exchange Platform.
Chambers are supplied with one box of #1.5 thickness, 25 mm coverslips and spare O-rings. Additionally, RC-47FSLP is supplied with
10 feet of PE-160 Polyethylene Tubing.
4.9 mm
RC-47FSLP
Volume:
118 µl
6.5 mm
Agar Bridge Well
18.5 mm
11.5 mm
4.9 mm
Volumes are for 1 mm solution height
Catalog No.
$
Model
Cell Biology
•
The RC-46SLP, RC-46SNLP, and
RC-47FSLP Chambers use a 25
mm round coverslip in a slotted
bath design to provide rapid
perfusion exchange. O-rings
seal the coverslip to the polycarbonate chamber and hold the
chamber securely in the aluminum base.
Product
BS4 64-0372
250.00 RC-46SLP
Quick Change Chamber
Slotted Bath
BS4 64-0373
250.00 RC-46SNLP Quick Change Chamber
Narrow Slotted Bath
BS4 64-0374
450.00 RC-47FSLP Quick Change Chamber w/ Field
Stimulation
Accessories/Replacement Parts
BS4 64-0375
595.00 QE-1
Quick Exchange Heated Base for
RC-40 Series
BS4 64-0705
14.00 CS25R
Coverslips 25 mm Dia.#1 Box/100
BS4 64-0715
15.00 CS25R15
Coverslips 25 mm Dia.#1.5 Box/100
BS4 64-1424
1,295.00 SIU-102
BS4 64-1425
76.00 CC-102
Stimulus Isolation Unit
Cable, male banana to 1 mm jacks
(for use with Warner field stimulation
chambers)
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M85
Micro-Incubation
Model QE-1 Quick Exchange Heated Platform with Perfusion
Quick Exchange Platform
Specialized Tools For Bioresearch
QE-1 shown with RC-42LP
Chamber
Open low profile platform for easy cell access
QE-1 with Series 40 Chamber
and Stage Adapter
Perfusion
Tube
•
Open design for easy
cell access
•
Removable perfusion
and suction tube holders
•
Quick exchange of
chamber or 35 mm dish
•
Designed for 35 mm
dishes and Series 40
chambers
•
Magnetic stainless steel
allows for the use of
magnetic holders
The Warner QE-1 Quick Exchange
Platform is a versatile base for the
RC-40 Series chambers, as well as
many popular 35 mm glass bottom dishes. The platform is at
home on both upright and inverted microscopes and includes
resistive heating elements. The
base has a 25 mm diameter aperture. Magnetic stainless steel
along the perimeter of the QE-1
permits the use of Warner’s MC-1
Magnetic Clamp Kit, which
includes a variety of holding tools.
Removable perfusion and suction tubes allow perfusion in any of the
RC-40 Imaging Chambers or 35 mm dishes. Suction and perfusion
tubes also feature set-screw locks for the horizontal location, and
adjustable presets on the vertical for setting the bath height. These
adjustments permit easy and exact positioning and repositioning of
the suction and perfusion tubes. A redesigned suction tube eliminates tidal action and fluid flutter, making aspiration very quiet.
Resistive heating of the QE-1 is provided by means of our
TC-324B/TC-344B Temperature Controllers and a CC-28 Cable.
Heating of perfusate is achieved using our SH-27B or SF-28 In-Line
Solution Heaters.
Adapter rings for Corning, Falcon and Willco 35 mm dishes are supplied to ensure a close fit in the QE-1.
Stage adapters are available for all major microscopes. Custom or
modified stage adapters are also available; call our Technical
Support Department for details.
Suction
Tube
Catalog No.
Heating
Elements
BS4 64-0375
$
Model
595.00 QE-1
Product
Quick Exchange Heated Base
w/Perfusion and Adapter Ring Kit
Accessories/Replacement Parts
BS4 64-0351
49.00 AR-4
Replacement Adapter Ring Kit
for 35 mm Dishes
BS4 64-0100
1,260.00 TC-324B
BS4 64-0101
2,016.00 TC-344B
BS4 64-0106
122.00 CC-28
BS4 64-0102
308.00 SH-27B
Solution Heater
BS4 64-0357
295.00 MCK-1
Magnetic Clamp Kit
Single Channel Heater
Controller
Dual Channel Heater Controller
Cable Assembly for Heater
Platforms
Adapter Rings for Corning,
Falcon, and Willco 35 mm Dishes
M86
11,000 Specialty Products to Enhance Your Bioresearch
Chambers for 35mm Cell Culture Dishes
PDMI-2 Open Perfusion Micro-Incubator
PDMI-2 Micro-Incubator
Typical Setup
PDMI-2 with TC-202A
Temperature Controller
(Not Included)
• Heats and cools
• ~5° to 50°C
• Maintains required cell
environment for hours:
- pH and O2
- temperature
• Peltier heat pump
• Easily change
perfusion fluid
• Makes temperature cycle
experiments easy
Flexible Temperature Range
The operating temperature range of the PDMI-2 Open Perfusion
Micro-Incubator is nominally ~5° to 50°C when used with the TC202A Temperature Controller, see page M125. Lower temperatures
can be achieved (approximately 10º below ambient) by using an
optional cool tube water-carrying coil which attaches to the perimeter
of the PDMI-2. Temperatures stabilize quickly throughout the operating range; temperature uniformity across the chamber is excellent.
Optimal and Flexible Perfusion
Two temperature controlled perfusion lines deliver perfusates through
the micro-incubator into the chamber a nominal rate of 3 ml per minute
each. Fluid level is maintained by placing an aspirator (model LU-ASP)
in the chamber. Fluid height is easily adjusted via a simple screw mechanism; a cleverly designed suction port eliminates fluid flutter thus making aspiration quiet; a magnetic base secures the aspirator to the microincubator at a user selectable convenient location.
Excellent pH Control
The PDMI-2 also provides gas flow over the top of the chamber for
pH control and improved temperature uniformity. The open design
provides excellent mechanical and optical access to the media.
Low Noise Conditions
A teflon well is provided with an Ag/AgCl electrode at its base to
allow for the formation of a salt bridge when making low noise
electrophysiological recordings.
Specifications
Chamber Options
Corning 35 mm Petri dishes, LU-CSD, MSC-TD
or MSC-PTD
Recommended Gas Flow
0.5 to 2.0 L/min
Temperature Range
~5° to 10°C below ambient to 50°C without
water cooling
Temperature Stability
±0.2°C with TC-202A, at 37°C with 1 ml/min perfusion
Temperature Gradient
Across Chamber
Built-In Temperature Sensor
2°C with 1.5 ml media volume, 1 ml/min perfusion
Peltier Device Current Rating
6 A max.
Media Perfusion Rates
Up to 3.0 ml/min
Overall Dimensions, H x D
17 x 152 mm (0.67 x 6.0 in)
Weight
0.5 kg (17.9 oz)
Microscope Stage Mounting
Call Harvard Apparatus for options
Catalog No.
BS4 65-0043
BS4 65-0058
$
Cell Biology
• Flexible chamber option
The PDMI-2 operates equally well
under static or perfused conditions. When operated with perfusion, the micro-incubator’s
unique design places flexible
tubes carrying inflowing perfusate
in contact with a temperature regulated Peltier heat pump driven
plate, thus heating or cooling the
incoming fluid to a command
temperature. This eliminates the
need for auxiliary preheating or
cooling. The Peltier plate heats or
cools the cell carrying central
chamber uniformly when no
perfusate is flowing.
Thermistor, 100 k Ω at 25°C - YSI 44011
Product
2,048.00 PDMI-2 Open Perfusion Micro-Incubator, comes
with LU-PD and LU-ASP (Adapter not included,
see page M93)
109.00 Copper Cooling Tube for PDMI-2
For TC-202A Bipolar Temperature Controller,
see page M125
For CO2 /pH Controller, see page M144
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M87
Chambers for 35mm Cell Culture Dishes
NEW
DH-35i Micro-Incubation System for 35 mm Cell Culture Dishes
Gas Port
Perfusion
Manifold
Suction
Outlet
Specialized Tools For Bioresearch
DH-35i fully assembled
Open setup for easy access
Heating
Elements
Dish Clamps
Modular Design
•
Designed for glass
bottom 35 mm dishes
•
Permits imaging,
temperature and gas
environment control
•
Compatible with Corning
and Falcon dishes and
Willco Wells.
•
Unique dish clamps allow
easy cell access
•
Compatible with RC-37
cell culture dish perfusion
chamber insert.
The introduction of glass bottomed 35 mm culture dishes
extended their use into areas
such as confocal microscopy, fluorescence microscopy, and high
resolution image analysis. The
DH-35i Culture Dish Incubator is
a system designed for imaging live
cells in an open culture dish for
easy access or in a closed dish for
gas atmosphere control.
DH-35i Incubators work well
with Willco Wells, Corning and
Falcon glass bottom 35 mm culture dishes. Adapter rings are supplied to ensure a close fit for the
supported dish. Dishes can be placed in the holder with or without
dish covers.
The holder is machined in three pieces. The midsection and top are
easily removed for dish replacement and enables the positioning of
electrodes at low angles. Resistive heating of the DH-35i is provided
via the optional Warner TC-324B Temperature Controller and a CC28 cable. Clamps are provided to ensure good contact between the
dish and the heated surface and to prevent unwanted movement. The
removable midsection is magnetically secured and has several ports
to allow multi-channel perfusion, atmospheric maintenance, and
aspiration of the dish. The base of the holder has a 19 mm aperture
and the removable cover features a 36 mm glass aperture.
M88
Adapter rings for Corning,
Falcon, and Willco 35 mm dishes
When combined with the Warner RC-37 Cell Culture Perfusion
Chamber Insert, a complete micro-environmental system is
achieved. This system is compatible with Warner’s SH-27B and SF-28
In-line Solution Heaters. Included with the system are an MP-2
Perfusion Manifold and a set of 4 culture dish adapter rings.
Stage adapters are available for all major microscopes. Custom or
modified stage adapters are also available; call our technical support
department for details. See page M114 for Series 20 Stage Adapters.
Catalog No.
BS4 64-0349
$
Model
895.00 DH-35i
Product
Culture Dish Incubation System
DH-35i
Accessories/Replacement Parts
BS4 64-0351
49.00 AR-4
Replacement Adapter Ring Kit for
DH-35i set of 4
BS4 64-0100
1,260.00 TC-324B
Heater Controller, Single Channel
BS4 64-0101
2,016.00 TC-344B
Heater Controller, Dual Channel
BS4 64-0106
122.00 CC-28
Cable Assembly for Heater Platforms
BS4 64-0102
324.00 SH-27B
Solution Heater
11,000 Specialty Products to Enhance Your Bioresearch
Chambers for 35mm Cell Culture Dishes
RC-37 Cell Culture Dish Perfusion Chamber Insert
Aspirator
Agar Bridge Well
RC-37W
Perfusion
Inlet Port
RC-37F
Culture Dish Perfusion
Designed for glass
bottom 35 mm
culture dishes
•
Permits imaging,
recording and perfusion
in the culture dish
•
Open and closed bath
designs
•
Incorporates Warner’s
diamond fluidics
•
Ideal for rapid screening
assays
The introduction of thin glass bottom culture dishes has expanded
the use of these popular tools
dramatically. Applications such
as live cell imaging, confocal
microscopy and time-lapsed photography are now possible. The
ability to exchange the extra-cellular environment by perfusion
within 35 mm dishes has been
difficult at best, until now.
The RC-37 Perfusion Chamber
Insert for cell culture dishes makes perfusion within these dishes a simple matter. Fluid is delivered to a perfusion inlet port and removed from
the chamber by means of a height adjustable aspirator.
Chambers are available to fit the Willco Wells, and the Corning or
Falcon 35 mm dishes. The chamber is compatible with Warner’s DH35 Culture Dish Heater and the DH-35i Culture Dish Incubator.
Catalog No.
$
Model
TOP VIEW
RC-37W
RC-37F
9.4 mm
15.2 mm
27 mm
34.5 mm
Volume: 110 µl
Volume: 220 µl
Cell Biology
•
Volumes are for 1 mm solution height
Product
BS4 64-0347
215.00 RC-37W
Chamber Insert for Willco/Corning
35 mm Dishes
BS4 64-0348
225.00 RC-37F
Chamber Insert for Falcon/Corning
35 mm Dishes
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M89
Chambers for 35mm Cell Culture Dishes
NEW
Models RC-37WC and RC-37FC – Closed Bath Chambers
Top Coverslip
Perfusion
Retainer
Outlet Port
Perfusion
Specialized Tools For Bioresearch
Inlet Port
RC-37FC
RC-37WC
•
Designed for glass
bottom 35 mm dishes
•
Permits imaging and
perfusion in the cell
culture dish
•
Incorporates Warner’s
diamond fluidics
•
Ideal for rapid screening
assays
The RC-37 family of perfusion
chamber
inserts
makes
perfusion of cell culture dishes a
simple matter. The closed bath
promotes an even and
continuous solution exchange
and eliminates changes in focus
due to alterations in bath height.
Studies involving physiological
bicarbonate buffers can be
performed with no gas loss at
the chamber interface.
Fluid is delivered to a 18 gauge perfusion inlet port and removed
from the chamber by means of an outlet port of the same gauge. The
top coverslip is held in place with a retainer and the dish becomes
the chamber bottom. A thin coating of silicone grease forms the seal.
The distance between top and bottom coverslips is 1 mm. The RC37WC uses 15 mm top coverslips and the RC-37FC works with 18
mm coverslips.
The RC-37WC fits in the Willco Wells dishes while the RC-37FC fits
the Falcon and Corning 35 mm dishes. Warner’s DH-35 Culture Dish
Heater may be used to heat the Corning and Willco Wells 35 mm
culture dishes. Falcon 35 mm dishes are compatible with Warner’s
DH-35i Culture Dish Incubator or the QE-1 Quick Change
Heated Base.
Both chambers are supplied with PE-160 Polyethylene Tubing
(10 ft.) and tools to remove the top coverslip retainer. The RC37WC
is supplied with one box of #1 thickness 15 mm coverslips and the
RC37FC is supplied with one box of #1 thickness 18 mm coverslips.
M90
TOP VIEW
RC-37WC
RC-37FC
15.2 mm
9.4 mm
27.4 mm
34.0 mm
Volume: 113 µl
Volume: 185 µl
Volumes are for 1 mm solution height
Catalog No.
$
Model
Product
BS4 64-0363
265.00 RC-37WC
Chamber Insert Closed for Willco
35 mm Dishes
BS4 64-0364
265.00 RC-37FC
Chamber Insert Closed for
Falcon/Corning 35 mm Dishes
Accessories/Replacement Parts
BS4 64-0703
12.00 CS15R
Coverslips 15 mm Dia. #1 Thickness,
Box of 100
BS4 64-0384
12.00 CS18R
Coverslips 18 mm Dia. #1 Thickness,
Box of 100
BS4 64-0758
108.00 D3522P
Glass Bottom Cell Culture Dishes,
pouch of 20
BS4 64-0762
548.00 D3522B
Glass Bottom Cell Culture Dishes,
pkg. of 120
BS4 64-0378
29.00 111-KIT
Silicone Grease Kit with Brushes
and Pallets
11,000 Specialty Products to Enhance Your Bioresearch
Chambers for 35mm Cell Culture Dishes
NEW
Models RC-37WS and RC-37FS - Perfusion Chamber Inserts
with Field Stimulation
Perfusion Outlet Port
Perfusion
Inlet Port
RC-37WS
Platinum
Wires
Agar Bridge
Well
Designed for glass
bottom 35 mm dishes
•
Permits imaging and
perfusion in the cell
culture dish
•
Platinum field
stimulation electrodes
•
Ideal for rapid
screening assays
The latest member of the RC-37
family of perfusion chamber
inserts is designed for applications requiring field stimulation
and includes a pair of platinum
electrodes. Both versions
feature a slotted bath to promote
a laminar solution flow and to
ensure an even electrical field.
TOP VIEW
24.0 mm
18.0 mm
Both chambers are supplied with 10 feet of PE-160
Polyethylene Tubing.
6.0 mm
6.0 mm
These models include a
perfusion inlet port and a 16
gauge height adjustable aspirator. An agar bridge well has been
added to accommodate a bath ground electrode. A thin coating of
silicone grease forms the seal between dish and chamber.
The RC-37WS fits in the Willco Wells dishes; the RC-37FS fits the
Falcon/Corning 35 mm dishes. Warner’s DH-35 Culture Dish Heater
may be used to heat the Corning and Wilco Wells 35 mm culture
dishes while Falcon 35 mm dishes are compatible with Warner’s DH35i Culture Dish Incubator or the QE-1 Quick Change Heated Base.
RC-37FS
RC-37WS
27.4 mm
Cell Biology
•
RC-37FS
34.0 mm
Volume: 105 µl
Volume: 144 µl
Volumes are for 1 mm solution height
Catalog No.
$
Model
Product
BS4 64-0365
450.00 RC-37WS
Chamber Insert Closed for Willco
35 mm Dishes
BS4 64-0366
450.00 RC-37FS
Chamber Insert Closed for
Falcon/Corning 35 mm Dishes
Accessories/Replacement Parts
BS4 64-0758
108.00 D3522P
Glass Bottom Cell Culture Dishes,
pouch of 20
BS4 64-0762
548.00 D3522B
Glass Bottom Cell Culture Dishes,
pkg. of 120
BS4 64-0378
29.00 111-KIT
BS4 64-1424
1,295.00 SIU-102
BS4 64-1425
76.00 CC-102
Silicone Grease Kit with Brushes and
Pallets
Stimulus Isolation Unit
Cable, male banana to 1 mm jacks
(for use with Warner field stimulation
chambers)
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M91
Chambers for 35mm Cell Culture Dishes
Specialized Tools For Bioresearch
NEW
Culture Dish Heater DH-35
Glass Bottom Cell Culture
Dishes (35 and 50 mm)
Glass Bottom
Cell Culture Dishes
DH-35 heaters work with Corning 35 mm tissue culture dishes
(Corning p/n 25000) and are designed for mounting on most
microscopes. The holder is machined in two pieces. The top half is
easily removed for replacement of dishes. Dishes can be placed in the
holder with or without dish covers. The holder is supplied with two
additional holder covers, one of which has openings to allow for
perfusion or insertion of probes or electrodes. The base of the holder
has a 19 mm diameter aperture. Stage adapters are available for many
microscopes, see pages M114 and M115. DH-35 is used with the
CC-28 Cable Assembly, see page M126. For 35 mm glass bottom
culture dishes, see right.
Catalog No.
$
Model
Product
•
Highest quality
construction, sterilized
•
#1 Cover glass for
microscope imaging
•
Glass covers complete
bottom
•
Compatible with DH-35
Dish Heater, see left
High quality polystyrene dishes
with No. 1 cover glass bottoms
are available in 35 mm and 50
mm diameters. They are available in both pouch pack
(20/pouch) and individual
blister pack (120/case). All
dishes are sterilized before
packaging. The 35 mm sizes are
compatible with the DH-35 Dish
Heater, see left.
Cover Glasses
BS4 64-0110
396.00
DH-35
35 mm Culture Dish Heater
BS4 64-0106
122.00
CC-28
Cable Assembly
Catalog No.
BS4 64-0350
30.00
AR-1
Adapter Rin for Willco Dish
Pouch-Pack Dishes, 20 Sterilized Dishes/Pouch
$
Model
Dish
Dimensions, D x H Glass Aperture
Stage Adapters for DH-35
Stage Adapter Microscope
BS4 64-0757
D3512P
35 x 9.9 mm
12 mm
BS4 64-0291
67.00
SA-NIK
Nikon Diaphot,TE200 & TE300
BS4 64-0758
108.00 D3522P
35 x 9.9 mm
22 mm
BS4 64-0295
67.00
SA-OLY/2
Olympus IMT-2, IX & BX50WI
BS4 64-0759
D5030P
50 x 7.2 mm
30 mm
BS4 64-0296
105.00
SA-20LZ
Zeiss Axiovert
BS4 64-0760
108.00 D5040P
50 x 7.2 mm
40 mm
BS4 64-0296
105.00
SA-20LZ
Leica DMIRB & DMIL
Blister-Pack Dishes, Individual Packaged Sterile Dishes, Case of 120
For other microscopes, please consult our technical support department.
BS4 64-0761
548.00 D3512B
35 x 9.9 mm
BS4 64-0762
D3522B
35 x 9.9 mm
12 mm
22 mm
BS4 64-0763
548.00 D5030B
50 x 7.2 mm
30 mm
BS4 64-0764
D5040B
50 x 7.2 mm
40 mm
Note: 35 mm Dishes supplied with Triple Vented Lids for optimum ventilation. 50 mm
Dishes have low side walls to facilitate microinjection.
For Single and Dual-Channel Heater Controllers,
see page M126.
M92
11,000 Specialty Products to Enhance Your Bioresearch
Chambers for 35mm Cell Culture Dishes
Micro-Incubators Accessories
Catalog No.
Zeiss Fixing
Platform
BS4 65-0047
BS4 65-0052
Zeiss, Nikon
and Olympus
Alignment
Rings
Adapters
Harvard Apparatus offers a wide range of alignment rings and fixing
platforms to adapt micro-incubators to all major microscope brands.
Catalog No.
$
$
Model
Product
Miscellaneous
Model
114.00 LU-ASP
36.00 MI-M
Leiden Aspirator, Cover Slip Dish
Fluid Level Setting Device, 38 mm H
Mini Magnets for Top Surface of
Micro-Incubators (LU-CB-1, PDMI-2 or
PSMI), set of 4
BS4 65-0053
366.00 PS-CSD
BS4 65-0054
29.00 LU-PD
Patch Slice Cover Slip Dish for PSMI
Sleeve of 35 mm Petri Dishes,
pkg. of 20
BS4 65-0055
64.00 BSC-TH
Magnetic Thermistor Holder for
LU-CB-1
Thermistors
BS4 65-0057
327.00 BSC-T3
Bath Thermistor for Use with PDMI-2,
PSMI, LU-CB1, and TC-202A
BS4 65-0056
327.00 BSC-T3A
Bath Thermistor for use with
LU-CPC-CEH and TC-202A
BS4 65-0098
162.00 BSC-T2
Replacement-Bath Thermistor for
Obsolete TC-102
BS4 65-0016
317.00 BSC-T2A
Replacement-Bath Thermistor for Use
with LU-CPC-CEH and TC-102
BS4 65-0058
109.00 PDMI-TUBE PDMI-2 Copper Cooling Tube Coil
(Shaped to Conform to Fin Channel)
with Open Ends
Product
Micro-Incubator Replacement Parts
BS4 65-0059
107.00 PDMI-ARN
PDMI-2/PSMI Alignment Ring for
Nikon Diaphot (Old or 300/200) TMD
Stage, 4.242 x .071 in (D x Thickness)
BS4 65-0066
12.00 D-ORING-1
BS4 65-0060
102.00 PDMI-ARO
PDMI-2/PSMI Alignment Ring for
Olympus IX50/70 or IMT-2 Fixed
Stage, 4.320 x .071 in (D x Thickness)
Cover Slip Dish O-Rings Replacement
for LU-CSD or MSC-TD, 1 Small
(approx. 23.80 mm OD) and 1 Large
(approx. 29.80 mm OD), set of 2
BS4 65-0067
20.00 D-ORING-2
PDMI-2/PSMI Alignment Ring
for Zeiss Axiovert Gliding or Rotary
Stages, 4.046 x .071 in
(D x Thickness)
Cover Slip Dish O-Rings,
Replacement for MSC-PTD, 1 Small
(approx. 20.80 mm OD) and 1 Large
(approx. 29.80 mm OD), set of 2
BS4 65-0068
43.00 CS-GLASS-1 Glass Cover Slips (#1) for LU-CSD or
MSC-PTD, 0.13 to 0.17 x 25 mm
(Thickness x D), pkg. of 100
BS4 65-0069
37.00 CS-GLASS-2 Glass Cover Slips (#2) for LU-CSD or
MSC-PTD, 0.18 to 0.25 x 25 mm
(Thickness x D), pkg. of 100
BS4 65-0061
BS4 65-0062
89.00 PDMI-ARZ
402.00 PDMI-FPL
PDMI-2/PSMI Fixing Platform for
Leica DAS Mikroskop DMIL and
DMIRB/E with Attachable
Mechanical Stage
BS4 65-0063
402.00 PDMI-FPZ
PDMI-2/PSMI Fixing Platform for
Zeiss Axiovert with Attachable
Mechanical Stage, M-Type Stage
BS4 65-0070
22.00 MI-T
Perfusion/Aspiration Teflon Tubing
Replace-ments for PDMI-2/PSMI, 2 ft
L x 26 AWG, pkg. of 8
BS4 65-0064
402.00 LU-FPL
LU-CB-1 Fixing Platform for Leica
DAS Mikroskop DMIL and DMIRB/E
with Attachable Mechanical Stage
BS4 65-0071
17.00 LU-CPC-P
Silicone Rubber Plugs for LU-CPC or
LU-CPC-CEH, set of 4
BS4 65-0072
81.00 TC-CA
BS4 65-0065
402.00 LU-FPZ
LU-CB-1 Fixing Platform for Zeiss
Axiovert with Attachable Mechanical
Stage, M-Type Stage
Replacement Output Cable to Connect
TC-102 Temperature Controller to
LU-CB-1 or LU-CPC-CEH
BS4 65-0093
108.00 PDMI-ARL
Alignment Ring for Leica Microscope,
88 x 1.8 mm (OD x Thickness)
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
Cell Biology
Alignment Rings and Fixing Platforms
M93
Special Chambers Systems
Confocal Imaging Chamber RC-30
These Confocal Imaging Chambers are supplied with six pre-cut 250
µm thick gaskets, two large baths (133 µl volume), two slotted baths
(25.6 µl volume), two blank for customized coverslips (22 x 30 mm
#1 and 22 x 40 mm #1), pkg. of 50 each, and PE-90 Tubing (3.3 m).
Specialized Tools For Bioresearch
Specifications
9.5 x 82 mm D
Weight
<90 g, assembled with optional heaters
Base and Pressure Plate
Aluminum, black anodized
Top Coverslip Plate
Polycarbonate
Gaskets
Silicone Rubber
Input/Output Tubing
PE-90
Coverslips (#1)
Top: 22 x 30 mm; Bottom: 22 x 40 mm
Catalog No.
$
Model
Gasket
Product
BS4 64-0320
938.00 RC-30
Confocal Imaging Chamber
BS4 64-0321
977.00 RC-30HV
Confocal Imaging Chamber with
Heaters
Optional Accessories
BS4 64-0322
83.00 SA-30NIK
The RC-30 is a low profile chamber with special features for confocal
imaging. The chamber bath has a variable volume and fast exchange
and is formed with a silicone gasket sandwiched between the top and
bottom cover slips. Pre-cut 250 µm thick gasket forms are supplied;
one wide cut to use the full aperture window (17.7 mm D), and a slotted version for faster solution exchange. Blank gasket forms are also
supplied permitting customized bath shape required for the application. Gaskets with 375 µm thickness are also available. The bath has
a large viewing area with an aperture of 17.7 mm D. The chamber
bottom is beveled to permit maximum access to the bottom coverslip.
The chamber is reversible and may be mounted in the stage adapter
to accommodate both upright and inverted microscopes. The RC30HV is an optional heater version, which includes a pair of resistive
heaters mounted to the chamber base. Heaters are compatible with
Warner TC324B / TC344B Heater Controllers, see page M126.
M94
Physical Dimensions
Stage Adapters for Nikon
Diaphot/TE200/TE300*
BS4 64-0323
168.00 SA-30TMS8 Stage Adapters for Nikon TMS
with 8 x 12 cm Cutout*
BS4 64-0324
160.00 SA-30TMS9 Stage Adapters for Nikon TMS
with 9 x 13 cm Cutout*
BS4 64-0325
79.00 SA-30/OLY2 Stage Adapters for Olympus
IMT-2/IX50/IX70/BX50WI*
BS4 64-0326
128.00 SA-30LZ w/ Stage Adapters for Zeiss Axiovert*
211 x 230 Specimen Stage
BS4 64-0327
139.00 SA-30KZ w/ Stage Adapters for Zeiss Axiovert*
85 x 130 Mechanical (K) Stage
BS4 64-0326
128.00 SA-30LZ
Stage Adapters for Leica DMIRB/E
with Plane Stage*
BS4 64-0328
161.00 SA-30L3P
Stage Adapters for Leica MIRB/E
with 3-Plate Mechanical Stage*
BS4 64-0329
161.00 SA-30PLI
For Prior & Ludl Motorized Stage*
BS4 64-0330
29.00 GS-30L/10
Replacement Gaskets for Large
Bath, 250 µm Thickness, pkg. of 10
BS4 64-0331
29.00 GS-30L/15
Replacement Gaskets for Large
Bath, 375 µm Thickness, pkg. of 10
BS4 64-0332
29.00 GS-30S/10
Replacement Gaskets for Slotted
Bath, 250 µm Thickness, pkg. of 10
BS4 64-0333
29.00 GS-30S/15
Replacement Gaskets for Slotted
Bath, 375 µm Thickness, pkg. of 10
BS4 64-0334
29.00 GS-30B/10
Replacement Gaskets for Blank,
250 µm Thickness, pkg. of 10
BS4 64-0335
29.00 GS-30B/15
Replacement Gaskets for Blank,
375 µm Thickness, pkg. of 10
BS4 64-0341
29.00 GS-30B/4
Replacement Gaskets for Blanks,
150 µm Thickness, pkg. of 10
BS4 64-0342
29.00 GS-30L/4
Replacement Gaskets for Large
Bath, 150 µm Thickness, pkg. of 10
BS4 64-0343
29.00 GS-30S/4
Replacement Gaskets for Slotted
Bath, 150 µm Thickness, pkg. of 10
*Note: see pages M114 and M115 for Stage Adapter drawings. Exterior dimensions for
corresponding models (SA-20 vs SA-30) are identical.
11,000 Specialty Products to Enhance Your Bioresearch
Special Chambers Systems
NEW
Model RC-50 Imaging Chamber for Transepithelial Studies
Pressure
Plate Wrench
Pressure
Plate
Top
Coverslip
Retaining
Ring
Top Coverslip
25 mm
RC-50
Low profile, Horizontally
Mounted Ussing Chamber
A #1 glass coverslip forms both the top and bottom of the chamber,
allowing it to be used with both inverted and upright microscopes.
While the chamber bottom is beveled to permit maximum access to
the bottom coverslip, the chamber body can also be inverted to allow
access to the other side. The viewing aperture in the chamber basolateral section is 17.7 mm in diameter and the viewing aperture in
the chamber apical section is 22.8 mm.
The RC-50 includes a pair of resistive heater elements mounted to
the chamber base. Heater elements are compatible with Warner
TC-324B and TC-344B Heater Controllers.
Specifications
Physical Dimensions
12.4 mm H x 82 mm D
Weight
<110 g, assembled with heaters
Platform and Pressure Plate
Aluminum, black anodized
Coverslip Plates
Polycarbonate
Input/Output Tubing
PE-90
Coverslips (#1)
Top: 25 mm; Bottom: 22 x 40 mm
Open Bath Cover
Perfusion
Port
Basolateral
Perfusion
Port
Suture Pins
Bottom
Coverslip
22 x 40 mm
Basolateral
Chamber
Platform
Catalog No.
BS4 64-0605
$
Model
1,995.00 RC-50
Product
Cell Biology
The RC-50 is a low profile, horizontally mounted Ussing chamber incorporating special fea• Custom round or slotted
tures for using confocal imagapertures to accommoing. The chamber can be configdate a variety of tissues
ured as a closed perfusion syssizes
tem using either gravity or pump
• Excellent optical access
feed. The apical (upper) surface
• Accommodates both
of the chamber can be used in
upright and inverted
an open or closed configuration.
microscopes
The basolateral chamber has
been designed to facilitate placement and stretching of hard to handle tissue such as trachea. Suture
pins are provided to act as anchor points for suture lines.
•
Apical
Chamber
Confocal Imaging Chamber
Optional Accessories
BS4 64-0322
83.00 SA-30NIK
Stage Adapters for Nikon
Diaphot/TE200/TE300
BS4 64-0323
168.00 SA-30TMS8 Stage Adapters for Nikon TMS with
8 x 12 cm Cutout
BS4 64-0324
160.00 SA-30TMS9 Stage Adapters for Nikon TMS with
9 x 13 cm Cutout
BS4 64-0325
79.00 SA-30/OLY2 Stage Adapters for Olympus
IMT-2/IX50/IX70/BX50WI
BS4 64-0326
128.00 SA-30LZ
Stage Adapters for Zeiss Axiovert
211 x 230 Specimen Stage
BS4 64-0327
139.00 SA-30KZ
Stage Adapters for Zeiss Axiovert
85 x 130 Mechanical (K) Stage
BS4 64-0326
128.00 SA-30LZ
Stage Adapters for Leica DMIRB/E
with Plane Stage
BS4 64-0328
161.00 SA-30L3P
Stage Adapters for Leica DMIRB/E
with 3-Plate Mechanical Stage
BS4 64-0329
161.00 SA-30PLI
For Prior & Ludl Motorized Stage
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M95
Special Chambers Systems
Specialized Tools For Bioresearch
Harvard/Medical Systems Microscope Stage Incubators
BS4 65-0101 CSMI Micro-Incubator
CSMI Chambered Slide Micro-Incubator
• Ideal for use with rectangular chambered slides and
chambered coverglass
• Provides comprehensive
micro-environment
maintenance, controlling
pH, temperature, perfusion
and gas atmosphere
• Using Peltier heat pump,
heats and cools over ~5°
to 50°C temperature range
• Accommodates up to 4
pre-temperature regulated
perfusion lines
• Provides temperature
regulated gas superfusion
• Magnetic base for easy
setup of different
configurations
The CSMI is a versatile microscope stage-incubator for cell/
tissue culture work. It is designed
to accommodate new rectangular
disposable Chamber Slides™ and
Chambered Coverglass from the
Nunc Lab-Tek® series, and the
Falcon® Cultureslide series from
Beckton Dickinson. The CSMI, in
combination with a matching low
noise TC-202A temperature
controller, facilitates long term
maintenance of tissue or cell cultures on a microscope stage. This
allows optical monitoring of dyes,
micro-injection, electrical recording, and micro-manipulation for
many hours or even days.
• Perfusate removal with
minimal disturbance of
fluid level using a unique
aspirator
The CSMI not only facilitates the
precise regulation and manipulation of bath temperature, but also
supports multi-channel perfusion
(up to 4 channels) and gas atmosphere maintenance, such as CO2 superfusion for pH control. The CSMI is based on a successful design of the
Medical Systems PDMI-2 micro-incubator for 35 mm Petri dishes. The
CSMI utilizes a Peltier thermo-electric device to regulate temperature
over a wide range both above and below ambient levels. Lower temperatures (approximately 10ºC below ambient) may be obtained by using an
optional cooling tube.
The CSMI fits on the stage of inverted microscopes from major microscope manufacturers including Zeiss, Nikon, Olympus and Leica.
Harvard Apparatus offers custom-made rectangular adapters to these
microscopes.
M96
Harvard Apparatus, Inc. offers a comprehensive line of
versatile Micro-Incubators and cell/tissue holding chambers
for use on the stages of microscopes. These Micro-Incubators
allow researchers to control temperature and pH, thus
significantly increasing the viability of the tissue culture.
Whether you have an inverted or upright microscope, it is
possible to achieve media heating and/or cooling under static
or dynamic conditions. We also have Micro-Incubators that
work in either an open or gas tight environment.
Four different Micro-Incubators and three different
companion chambers are described on the following pages
including our new CSMI micro-incubator for rectangular
slides. In addition, we offer a fifth micro-incubator system, our
model PSMI, for customers in need of the shallow depths and
low angles of attack typically required for patch slice studies.
The TC-202A temperature controller, see page M125, is available which is perfectly matched to the heating and cooling
requirements of all the Micro-Incubator systems.
Specifications
Temperature Range
~5° to 10°C below ambient to 50°C without water cooling
Disposable Chambers
Accommodated
1. Nalge Nunc chambered slides & cover slips
2. Becton Dickinson Falcon chambered slides
Microscopes
Accommodated
1. Zeiss Axiovert with attachable mechanical stage
2. Leica DAS Microscope DMIL and DMIRB/E with attached
mechanical stage
3. Nikon Diaphot
4. Olympus IX50/70 or IMT-2 fixed stage
Peltier TED Current Rating
6 A DC max
Built In Plate Thermistor
100 kΩ at 25°C — YSI 44011
Perfusion Inlet Lines
26 AWG Teflon capillary up to 4 lines can be installed
simultaneously
Perfusion Rate
3 ml/min nominal total
Perfusate Outlet
LU-ASP aspirator
Gas Port
1/16 in. barb
Gas Superfusion Rate
0.5 to 2.0 L/min
Weight
17.9 oz (0.5 kg)
Overall Dimensions
6.5 x 4 x 1 in (16.5 x 10.2 x 2.5 cm)
Catalog No.
$
Product
BS4 65-0101
2,975.00 CSMI Chambered Slide Micro-Incubator
BS4 65-0045
2,397.00 TC-202A Bipolar Temperature Controller,
see page M125
BS4 65-0103
136.00 Copper Cooling Tube for CSMI
For TC-202A Bipolar Temperature Controller,
see page M125.
For pH Controller, see page M144.
11,000 Specialty Products to Enhance Your Bioresearch
Special Chambers Systems
PSMI Patch Slice Micro-Incubator
BS4 65-0044 PSMI
Micro-Incubator
(Typical Setup)
•
BS4 65-0040 LU-CB1
Leiden Micro-Incubator
Small entry angles ideal
for patch slice recordings
•
Shallow fluid level minimizes pipette capacitance
•
Heats & Cools
•
~5° to 50°C
•
Peltier heat pump
•
Maintains cell conditions
for hours
The Patch Slice Micro-Incubator
System provides a complete solution to the challenge of electrophysiological study of synaptic
slice connections at physiological
temperatures using low noise
whole cell patch recording either
with or without water immersion
of the microscope objective in
the recording medium.
Specifications
Chamber Type
PS-CSD Cover Slip Dish
Temperature Stability
±0.2°C with TC-202A, at 37°C with 1 ml/min perfusion
Temperature Gradient
Across chamber: 2°C with 1.5 ml media volume, 1 ml/min
Temperature Range
5°-10°C below ambient to 50°C without water cooling
Built In Temp. Sensor
Thermistor type: 100 kΩ at 25°C YSI 44011
Peltier Device
Current rating: 6 A max
Media Perfusion Rates
Up to 3.0 ml/min
Dimensions, H x Dia
17 x 152 mm (0.67 x 6.0 in), overall
Weight
0.5 kg (17.9 oz)
BS4 65-0044
BS4 65-0109
$
•
Ambient to 50°C
•
Designed by top teachers
at Leiden University
•
Proven design in use for
nearly two decades
•
Open design for easy
cell access
The LU-CB1 Leiden MicroIncubator provides stable culture
conditions (temperature, pH,
aseptic isolation, and osmolarity)
during continuous or intermittent observations when media
temperatures between ambient
and 50°C are desired.
The LU-CBl is used primarily for static (no perfusion) chamber media
applications. It features excellent mechanical and optical access to
cells or tissue. Measuring probes (temperature, pH, etc.), recording
electrodes, and micropipettes can easily be manipulated in and out of
the chamber.
Aseptic isolation of the culture media can be provided by using a layer
of non-toxic mineral oil. A gas flow is directed over the oil to control
the micro-environment (including pH) and to minimize temperature
gradients across the chamber. It is possible to use the LU-CB1 as a perfused system by preheating the perfusate close to the desired temperature before it enters the chamber.
Specifications
Chamber Options
Coming 35 mm Petri dishes, LU-CSD, MSC-TD or MSC-PTD
Recommended Gas Flow
0.5 to 2.0 L/min
Temperature Range
Ambient to 50°C when used with TC-202A
Temperature Stability
±0.2°C when used with TC-202A
Temperature Gradient
Across Chamber
±0.3°C, gas flow of 1.5 L/min, 3.5 ml media volume
Heating Element
2.0 Ω Nichrome Coil
Dimensions, H x D
16 x 90 mm (0.63 x 3.54 in), overall
Weight
150 g (5.3 oz)
Microscope
Stage Mounting
Call Harvard Apparatus, Inc. for options
Catalog No.
$
Cell Biology
This micro-incubator is a shallow, annular assembly which surrounds
the central slice holding dish. Room temperature perfusant is delivered to the incubator which in turn delivers it to the dish at a precisely controlled temperature you select. The perfusant is removed (via
user supplied suction) by an included, height adjustable aspirator
(LU-ASP). Two independent channels of perfusant flow through the
PSMI into the chamber via replaceable plastic tubing of low volume
(<100 µl) to allow rapid switching of media via an upstream valve for
pharmacological studies. A separate gas inlet provides temperature
controlled gas across the dish. The TC-202A provides temperature
control (~5° to 50°C) using the micro-incubator warming/cooling
plate as the control point. Lower temperatures (approximately 10ºC
below ambient) may be obtained by using an optional cooling tube. The
cover slip dish (PS-CSD) consists of two 22 mm diameter cover slips
held in a circular frame. The slice sits on the top cover slip with the
immersion objective reaching the fluid surface. The bottom cover slip
prevents condensation for optimal illumination and viewing. To enable
placement of the patch electrode, entry angles as small as 15° can be
achieved for slice access.
Catalog No.
LU-CB1 Leiden Micro-Incubator
Product
BS4 65-0040
1,228.00 LU-CB1 Leiden Micro-Incubator, with BSC-TH,
Tissue Pins, and 3 Alignment Rings (Nikon,
Olympus and Zeiss) and Output Cable
BS4 65-0045
2,397.00 TC-202A Bipolar Temperature Controller
BS4 65-0100
89.00 Output Cable TC-202A to Microincubator
Product
2,288.00 PSMI Patch Slice Micro-Incubator with PS-CSD
For pH Controller, see page M144.
109.00 Copper Cooling Tube for PSMI
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M97
Special Chambers Systems
LU-CPC-CEH Leiden Closed
Perfusion Micro-Incubator
Silicone Grease Kit
Specialized Tools For Bioresearch
BS4 65-0041 LU-CPC-CEH Micro-Incubator
Recommended for all Warner Chambers
This micro-incubator features a
short optical path between
coverslips to allow single cell flu• Controls atmospheric
conditions: P02 and O2
orescence measurements under
maximum objective magnifica• Ambient to 50° C
tion using either an inverted or
• 8 ports for perfusion
upright microscope. Media
or sensors
access is flexible. Four symmetrically placed ports are provided
with plugs which are designed so that direct streaming of perfusate
onto the cells is avoided. Plug rotation creates different degrees of
mixing. There are four additional ports for inserting special purpose
probes (e.g. temperature, pO2) or hypodermic needles to introduce
reagents rapidly.
• Sealed gas type chamber
We have determined that an effective method of applying silicone
lubricant used to form a seal between the coverslip and chamber is
an artist’s acrylic brush. With that in mind, we have put together
a convenient package that includes a tube of Dow Corning® 111 Valve
Lubricant & Sealant, #2 and #4 brushes and several pallets to aid in
applying the grease.
Catalog No.
$
BS4 64-0378
29.00 111-Kit
NEW
Model
Product
Silicone Grease Kit with Brushes
and Pallets
Silicone Lubricant
The Leiden closed perfusion micro-incubator uses a nichrome heating
element which surrounds the chamber. It requires the TC-202A
Temperature Controller. Micro-incubator supplied with output cable and
temperature probe.
Specifications
Cover Slips
#1 or 2, 25 mm D (Model # CS-GLASS-1 or CS-GLASS-2)
Distance Between
Cover Slips
14 mm (0.55 in)
Temperature Range
Ambient to 50°C with TC-202A
Heating Element
2.0 Ω Nichrome heating coil
Fluid Inlets/Outlets
4 (0.83 X 2.36 in)
Other Inlets for Probes
4 (0.83 X 2.36 in)
Dimensions, H x D
21 x 60 mm (0.83 x 2.36 in)
Weight
60 g (2.1 oz)
Dish Material
Eriflon™ and Stainless Steel
Catalog No.
BS4 65-0041
M98
$
Product
922.00 LU-CPC-CEH Leiden Closed Perfusion MicroIncubator Chamber with Heating
Sealing between coverslip and chamber is most often done with a
high purity silicone lubricant such as stopcock or vacuum grease.
The Dow Corning® 111 Valve Lubricant & Sealant retains viscosity at
elevated temperatures better than others we tested. The 111 sealant
is particularly recommend for heated perfusion systems.
Catalog No.
BS4 64-0275
$
Product
18.00 Silicone Lubricant
11,000 Specialty Products to Enhance Your Bioresearch
Special Chambers Systems
Series 20 Perfusion Chambers for Imaging and Recording
Series 20 Chamber,
Platform and Stage Adapter
L
View thru single
coverslip
•
Laminar perfusion flow
•
Small volumes,
fast exchange
•
Use with most
microscopes
•
Open and closed
bath types
Perfusion Flow
W
Assembly of
Chamber
The chamber is prepared by
applying a thin bead of vacuum grease to the chamber
bottom. The sample coverslip (sample side up) is then
attached to the chamber.
This assembly is then placed
in the platform and clamped
to provide a seal between
coverslip and chamber.
Mounting on the microscope is done with the
appropriate stage adapter.
The installation is completed by connecting the perfusion lines and any heater
cabling required.
Assembly of the closed type
chambers includes installation of the top coverslip
which precedes the steps
outlined above.
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Cell Biology
•
Modular Design
Series 20 chambers are a family of models designed
to fill a large number of imaging and recording
needs. The modular design consists of two parts; a
polycarbonate chamber (insert) and an aluminum
platform (holder). The platform clamps the chamber
providing a seal between the chamber and coverslip.
Heater type platforms are used where chamber
warming is required. The design of the platforms permits quick removal of the chamber for exchange of
the coverslip. A variety of bath sizes and shapes are
offered to provide a ready solution to many research
requirements. Most models are stocked in depth for
fast delivery.
Single Coverslip Design
A common feature of Series 20 chambers is the use of
a disposable coverslip as the bottom or floor of the
chamber. In many cases, this same coverslip will contain the cells to be studied. Thus, when used with an
inverted microscope, the image is viewed through a
single #1 coverslip thickness, nominally 0.15 mm.
Laminar Flow-Fast Exchange
A diamond-shape bath is used in many Series 20
chambers (models thru RC-26) to provide a linear
solution flow. Studies performed at Yale and Stanford
Universities showed that this shape produced a laminar flow across the bath. The general shape is shown
below and specific dimensions are provided in the
following pages. In general, the lower the ratio of bath
width (W) to length (L), the better the flow characteristics. Models with small bath volumes can have
exchange times measured in seconds even with flow
rates less than 1 ml/min. Newer models starting with
RC-27 use a slotted bath shape. They are designed for
slice studies and other applications requiring a
larger bath size.
Perfusing the Chambers
Perfusion can be a pump-driven or gravity-fed system. Flow rates of 10 ml/min or greater are possible
but flows of 0.5 to 5 ml/min are the norm. Standard
PE-160 polyethylene tubing (or equivalent) inserts
directly into the input port. Solutions are removed by
suction with either a vacuum source or pump. Open
bath chambers are equipped with a stainless steel suction tube which is adjustable for control of solution
height. Multiple perfusion lines can be connected to
the chambers by using one of the MP or MPP Series
manifolds, see page M140.
M99
Special Chambers Systems
Series 20 Closed Bath Imaging Chambers
RC-21B with
P-2 Platform
Specialized Tools For Bioresearch
RC-20 in P-5
Platform
RC-21BRFS with
P-2 Platform
New Improved Suction Tube Holder
Series 20 Imaging Chambers use coverslips at
both top and bottom to enclose the bath area. The
closed bath promotes an even and continuous
solution exchange and eliminates changes in
focus due to alterations in bath height. Studies
involving physiological bicarbonate buffers can
be performed with no gas loss at the chamber
interface. The closed design allows the use of permeable supports. With a laminar flow, minimal
distortion of the support will occur. Closed chambers have been used in fluorescent, calcium and
time-lapse imaging studies.
All series 20 chambers equipped with a stainless
steel suction tube now include a suction tube
clamp. This insures the adjustable tube will not
move once the solution height is set. For Series 20
Chamber replacement parts, see page M117
Small Volume
Imaging Chambers
RC-20 (48 µl)
& RC-20H (90 µl)
RC-20/RC-20H
RC-20 and RC-20H are very small
volume imaging chambers featuring fast perfusion exchange and
short working distance. Two
CR-15
15 mm diameter coverslips
enclose the bath. Distance
between top and bottom coverslips is 1 mm. The top coverslip
is held in place with a retainer.
The sample coverslip is placed on the chamber bottom and the assembled chamber
is placed in the chamber platform.
Two bath sizes are available. The smaller and narrower bath of the RC-20 insures
a linear flow and fast solution exchange while the larger RC-20H provides a larger viewing area The additional port at the bath input may be used to inject substances into the bath or to remove gas bubbles. Order with P-5 or PH-5 platform,
see page M112.
Catalog No.
M100
$
Model
BS4 64-0222
320.00 RC-20
BS4 64-0223
320.00 RC-20H
BS4 64-0276
29.00 CR-15
Product
Small Volume Imaging Chamber, 48 µl
Small Volume Imaging Chamber, 90 µl
Replacement Retaining Ring
11,000 Specialty Products to Enhance Your Bioresearch
Special Chambers Systems
Series 20 Closed Bath Imaging Chambers
RC-21BRFS
Large Bath Imaging Chambers
RC-21B & RC-21BR
Cell Biology
Large Bath Imaging Chambers
RC-21B & RC-21BR (360 µl/2.5 mm)
Imaging Chamber with Field Stimulation
RC-21BRFS (263 µl/2.5 mm)
These chambers provide a large viewing area and good access for
microscope optics at chamber top and bottom. The chambers are
identical except for the coverslips used; 22 mm square for RC-21B
and 25 mm round for RC-21BR. Distance between top and bottom
coverslip is 2.5 mm. Bath volume is 360 µl for 2.5 mm of solution.
An additional port at the bath input may be used to inject substances
or for removing bubbles. At the bath output, a length of tubing is
used as a back pressure control to insure a smooth perfusion flow.
Order with P-2 or PH-2 platform, see page M112.
RC-21BRFS is an imaging chamber with field stimulation electrodes. A
pair of platinum wires is epoxied into the parallel sides of the ovalshaped bath. The connecting wires are terminated in 1 mm pins. The
chamber uses a pair of 25 mm round coverslips. Bath volume is
260 µl for 2.5 mm of solution. The additional port at the chamber perfusion input may be used for removing bubbles or injecting a substance. At the bath output, a length of tubing is used as a back pressure
control to insure a smooth perfusion flow. Order with P-2 or PH-2 platform, see page M112.
Catalog No.
Product
Catalog No.
BS4 64-0224
304.00 RC-21B
$
Model
Large Bath Imaging Chamber
BS4 64-0226
BS4 64-0225
304.00 RC-21BR
Large Bath Imaging Chamber
$
Model
Product
392.00 RC-21BRFS Imaging Chamber with
Field Stimulation
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M101
Special Chambers Systems
Series 20 Open Bath Recording/Imaging Chambers
Open bath models are designed for electrophysiological recording
and/or optical imaging, and are used in applications including
patch clamp, oocyte clamp, and physiological measurements on
cell cultures and tissue preparations. The low profile design permits
good electrode access to the bath. Agar bridge wells for reference
electrodes are provided.
Specialized Tools For Bioresearch
RC-24E In P1
M102
RC27L In PH6D
TOP VIEW
Bath
Suction Tube
Perfusion
Inlet Port
RC-22C W/P1
RC-26 W/Slice Anchor
In P1
RC-25F In P-3
Wick Slot
3.8 mm
17.0 mm
Volume: 143 µl
13 mm
Volume is for 1 mm solution
Model RC-21BDW Chamber for 25 mm
Round Coverslip (143 µl)
The RC-21BRW is a new addition to the Series 20 family. It is an open
chamber using the popular 25 mm round coverslip. Constant
solution level and quiet operation are assured with the use of a wick
between the bath and suction well. RC-21BRW is used with either the
P-2 or PH-2 platform, see page M112. Bath volume is 240 µl for
1 mm solution height.
The RC-21BDW is an open
chamber using the popular 25
mm round coverslip. Constant
solution level and quiet opera• Open chamber using
25 mm coverslips
tion are assured with the use of
• 143 µl bath volume for
a wick between the bath and
1 mm solution height
suction well. The RC-21BRW
is used with either the P-2 or
PH-2 Platform. Bath volume is
143 µl for 1 mm solution height. Standard accessories supplied are
25 mm #1 coverslips and PE-160 Tubing (10 ft.).
Catalog No.
Catalog No.
•
NEW
Chamber for 25 mm Round Coverslip
RC-21BRW (240 µl)
$
Model
Product
Diamond-shaped bath
for a linear solution flow
$
Model
BS4 64-0227
304.00 RC-21BRW Open Chamber for 25 mm Round
Coverslip
BS4 64-0362
BS4 64-0278
315.00 P-2
Platform
Accessories/Replacement Parts
BS4 64-0285
343.00 PH-2
Heater Platform
BS4 64-0705
289.00 RC-21BDW
14.00 CS25R
Product
Open Chamber for 25 mm Round
Coverslips
Coverslips 25 mm Dia. #1
Box of 100
11,000 Specialty Products to Enhance Your Bioresearch
Special Chambers Systems
Series 20 Open Bath Recording/Imaging Chambers
RC-24/RC-24E
RC-22/RC-22C
Volume:
lume: 113 µl
Volume:
lume: 138 µl
Recording Chambers for Tissue Slice RC-22
(113 µl) & RC-22C (138 µl)
Fast Exchange Recording Chamber
RC-24 (40 µl) & RC-24E (50 µl)
Small bath chambers for tissue and brain slice studies. Slices are
held down with stainless steel anchors with lycra threads at 1.5 mm
spacing. Anchor weight is 300 milligrams. One anchor is supplied
with each chamber. Bottom coverslip size is 22 x 40 mm. Order with
P-1 or PH-1 platform on page M112.
Product
The bath volume in these two models is the smallest of the open style
chambers. This design allows very fast solution exchange for a
variety of applications including patch clamp and studies in electrophysiology. The small bath volumes are especially useful when using
drugs in small quantities. Both models are used with 22 mm square
or 22 mm x 40 mm coverslips. Order with P-1 or PH-1 platform,
see page M112.
BS4 64-0228
304.00 RC-22
Recording Chamber for Tissue Slice
with SHD-22L/2 Anchor
Catalog No.
BS4 64-0229
304.00 RC-22C
Recording Chamber for Tissue Slice
with SHD-22CL/2 Anchor
Catalog No.
$
Model
Additional Anchors
BS4 64-0246
33.00 RC-22L/2
Slice Anchor for RC-22 (1.5 mm)
BS4 64-0247
33.00 RC-22CL/2
Slice Anchor for RC-22C (1.5 mm)
$
Model
Cell Biology
Volumes are for 1 mm solution
Product
BS4 64-0230
293.00 RC-24
Fast Exchange Recording Chamber
BS4 64-0231
293.00 RC-24E
Fast Exchange Recording Chamber
For Patch Clamps, see pages H2 to H5.
For Oocyte Clamps, see pages H20 to H26.
Slice Anchor Kits available,
see pages M116 and M117.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M103
Special Chambers Systems
Series 20 Open Bath
Recording/Imaging Chambers
Recording Chamber
Specialized Tools For Bioresearch
RC-25/RC-25F
TOP VIEW
Agar Bridge Well
Suction
Tube Slot
Bath
Perfusion Inlet Port
Suction Reservoir
3.8 mm
15.0 mm
6 mm
Volume: 54 µl for 1 mm solution height
Model RC-24N Fast Exchange Recording
Chamber (54 µl)
This chamber features the smallest bath volume of the open style
chambers. This design allows
very fast solution exchange for a
• 54 µl bath volume for 1
mm solution height
variety of applications including
patch clamp and studies in elec• Suction tube clamp
trophysiology. Small bath volumes are especially useful when using drugs in small quantities.
A 22 mm x 40 mm coverslip forms the chamber bottom.
•
Redesigned to improve
flow characteristics
Order with P-1 or PH-1 Platform. Standard accessories supplied are
22 x 40 mm #1 coverslips and PE-160 Tubing (10 ft.).
Catalog No.
BS4 64-0381
$
Model
293.00 RC-24N
Product
Fast Exchange Recording Chamber
Accessories/Replacement Parts
BS4 64-0707
M104
12.00 CS-22/40
Coverslips 22 x 40 mm #1,
Box of 50
Volumes are for 1 mm solution
Round Coverslip Chambers
RC-25 (90 µl) & RC-25F (133 µl)
The bottom of these chambers is formed from standard size round
coverslips, a widely used culture support for cells destined for physiological recording and/or imaging. RC-25 works with either 12 or
13 mm diameter coverslips and RC-25F uses 15 mm coverslips.
RC-25 is ordered with P-3 or PH-3 platform. RC-25F is ordered with
P-4 or PH-4 platform, see page M112 for platform information.
These chambers are not designed for coverslip insertion from the top.
Catalog No.
$
Model
Product
BS4 64-0232
293.00 RC-25
Round Coverslip Recording Chamber
(90 µl)
BS4 64-0233
293.00 RC-25F
Round Coverslip Recording Chamber
(133 µl)
11,000 Specialty Products to Enhance Your Bioresearch
Special Chambers Systems
Series 20 Open Bath Recording/Imaging Chambers
RC-26GLP
RC-26/RC-26G
Cell Biology
Large Bath Recording Chamber
RC-26 (170 µl) & RC-26G (234 µl)
Low Profile Large Bath Recording Chamber
RC-26GLP (234 µl)
These chambers accommodate larger slices and preparations and permit
excellent access for electrodes. The larger baths also allow for insertion
of a round coverslip from the top; a 12 mm in RC-26 and a 15 mm in RC26G. Optional slice hold down anchors are offered for both models. The
anchors are stainless steel frames with lycra threads at 2 mm spacing and
weigh approximately 500 milligrams. Both models use a 22 x 40 mm coverslip on the bottom. Order with P-1 or PH-1 platform on page M112.
RC-26GLP is a low profile version of the RC-26G. The bath area of the
chamber has been milled to a height of only 2 mm. The lower profile
provides extra clearance for upright microscopes. Bottom coverslip
size is 22 x 40 mm. Order with P-1 or PH-1 platform, see page M112.
Catalog No.
$
Model
Product
BS4 64-0234
293.00 RC-26
Large Bath Recording Chamber
BS4 64-0235
293.00 RC-26G
Large Bath Recording Chamber
Optional Slice Anchors
BS4 64-0252
33.00 SHD-26H/2
BS4 64-0255
33.00 SHD-26GH2 Slice Anchor for RC-26G and
RC-26GLP (2 mm)
Catalog No.
BS4 64-0236
$
Model
293.00 RC-26GPL
Product
Low Profile Large Bath Recording
Chamber
Optional Slice Anchors
BS4 64-0252
33.00 SHD-26H/2
Slice Anchor for RC-26 (2 mm)
BS4 64-0255
33.00 SHD-26GH2 Slice Anchor for RC-26G and
RC-26GLP (2 mm)
Slice Anchor for RC-26 (2 mm)
Other Anchor Weights and Spacings available,
see page M116
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M105
Special Chambers Systems
Series 20 Open Bath Recording/Imaging Chambers
RC-26Z
Specialized Tools For Bioresearch
RC-27
Large Bath Chamber for Oocyte Recording
RC-26Z (170 µl)
Similar to the RC-26 this chamber has two additional features for
oocyte recording; the dam height in the suction well is increased to
2.5 mm to provide an adequate solution level in the bath and a
second agar bridge well has been added to accommodate bath clamp
electrodes. Bottom coverslip size is 22 x 50 mm. Ordered with P-1
or PH-1 platform, see page M112.
Catalog No.
BS4 64-0237
M106
$
Model
304.00 RC-26Z
Very Large Bath Chamber RC-27 (413 µl)
This chamber and its evolutions on the following pages feature a
slotted shaped bath. The rectangular bath of the RC-27 will
accommodate large specimens or the insertion of coverslips (up to
12 x 24 mm) typically used for organotypic slice culture studies.
An adjustable spreader plate at the bath input provides a uniform
solution flow across the bath. The bottom coverslip size is 24 x 50
mm. Order with P-6 or PH-6 platform, see page M112. Slice anchor
kit available, see page M117.
Product
Large Bath Oocyte Recording
Chamber RC-26Z
Catalog No.
BS4 64-0238
$
Model
320.00 RC-27
Product
Very Large Bath Recording Chamber
11,000 Specialty Products to Enhance Your Bioresearch
Special Chambers Systems
Series 20 Open Bath Recording/Imaging Chambers
RC-27N
Narrow Bath Chamber RC-27N and
Field Stimulation Chamber RC-27NE
Model RC-27N is a modified version of the RC-27 chamber with a
narrower bath and smaller bath volume. Laminar solution flow is
more stable than in the wider bath models.
Model RC-27NE is for applications requiring field stimulation and is
supplied with a pair of platinum electrodes. The electrodes are
installed along the two parallel sides of the bath. Electrode leads are
approximately 16 cm long and terminated with 1 mm diameter pins.
The RC-27N and RC-27NE chambers use a 24 x 50 mm coverslip on
the bottom. Both models are used with either P-6 or PH-6 (heater)
platforms, see page M112.
RC-27NE
Specifications
RC-27N and RC-27NE
Material
Polycarbonate
Bath Dimensions:
Length x Width
Specimen Area
Volume/mm of Solution Height
37 x 8 mm
28 x 8 mm
305 µl per mm of solution
Standard Accessories Supplied:
PE-160 Tubing (10 ft)
24 x 50 mm #1 Coverslip, pkg. of 50
Platforms Used With:
P-6 Plain Platform
PH-6 Heating Platform
Catalog No.
$
Model
Product
320.00 RC-27N
Narrow Bath Recording Chamber*
BS4 64-0240
414.00 RC-27NE
Narrow Bath Recording Chamber
with Field Stimulation*
*Note: RC-27N and RC-27NE Chambers used with either P-6 or PH-6 (Heater) Platforms
and Stage Adapter, see pages M112 to M115.
Cell Biology
BS4 64-0239
For our complete line of Platforms
and Adapters, see pages M112 to M115.
Slice Hold-Downs available in Kits,
see page M117.
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M107
Special Chambers Systems
Large Bath Chamber RC-27L
The RC-27L chamber is designed for slice studies where
solution above and below the slice is desired. The large
bath size and platform design also provide good access
for immersion optics and electrodes.
The slice rests on a support 0.5 mm above the coverslip
and is held in place by a slice anchor hold-down. Both
support and hold-down use fine lycra thread to secure
the slice in place allowing maximum access to the
preparation. Thread spacing is 1 mm on the slice support.
Specialized Tools For Bioresearch
Choice of Slice Hold-Down
Two hold-down styles are available: a stainless steel “harp”
and a rectangular plastic frame. While the weight of the
metal harp holds the slice in place, the plastic frame relies
on a close apposition between itself and the side walls of
the bath. Hold-downs are available with thread spacing of
1, 1.5 and 2 mm, and are sold separately. See accessoreis
for selection of slice hold-down.
Specifications
Material
Polycarbonate
Bath Dimensions
Size, L x W
Specimen Area
Volume
38 x 18 mm
23 x 18 mm
560 µl per mm of solution height
Std. Accessories Supplied
1. SS-3 Slice Support
2. PE-160 Tubing (10')
3. 24 x 50 mm #1 Coverslip, pkg. of 50
Platforms Used With
1. P-6D Plain Platform
2. PH-6D Heater Platform
Catalog No.
BS4 64-0241
$
Model
314.00 RC-27L
Product
RC-27L Chamber
Accessories (Spare or Replacements)
Volume:
Volum
e: 560 µl
BS4 64-0273
31.00 SS-3
BS4 64-0256
35.00 SHD-27LH/10 Metal Slice
Hold-Down, 1 mm
BS4 64-0257
35.00 SHD-27LH/15 Metal Slice
Hold-Down, 1.5 mm
BS4 64-0258
35.00 SHD-27LH/2 Metal Slice
Hold-Down, 2 mm
BS4 64-0259
66.00 SHD-27LP/10 Plastic Slice
Hold-Down, 1 mm
BS4 64-0260
66.00 SHD-27LP/15 Plastic Slice
Hold-Down, 1.5 mm
BS4 64-0261
66.00 SHD-27LP/2 Plastic Slice
Hold-Down, 2 mm
Volume is for 1mm solution
Volume
SliceSupport
*Note: RC-27L Chambers require Platform P-6D of PH-6D, see page
M112, and Microscope Stage Adapter, see pages M114 and M115.
For a complete listing of individual
Slice Hold-Downs and kits, see pages M116 and M117
M108
11,000 Specialty Products to Enhance Your Bioresearch
Special Chambers Systems
Excised Patch Chamber RC-28
This chamber is designed for electrophysiological
recordings of excised patches. The dual bath construction permits exchange of the solution surrounding the
membrane patch without contamination of the entire
population. The chamber consists of two independent
bath compartments separated by a dam/reservoir which
functions as a gate and barrier to prevent mixture of the
solutions. The composition and exchange rate of each
bath can be controlled independently.
RC-28
After patching onto a cell in the larger bath compartment, the dam/reservoir is flooded with solution establishing a “solution bridge” between the two compartments. This enables secure transfer of the electrode
attached cell to the smaller compartment. The
dam/reservoir is then emptied by vacuum suction thus
separating the two baths.
Specifications
Polycarbonate
Small Bath Dimensions:
Length x Width
Specimen Area
Volume
28 x 3.8 mm
20 x 3.8 mm
111 µl per mm of solution height
Large Bath Dimensions:
Size, L x W
Specimen Area
Volume
28 x 9.5 mm
21 x 9.5 mm
234 µl per mm of solution height
Standard Accessories
Supplied
1. PE-160 Tubing 10 ft L
2. 24 x 50 mm #1 Coverslip, pkg. of 50
Platforms Used With
1. P-6 Plain Platform
2. PH-6 Heater Platform
Catalog No.
BS4 64-0243
$
Model
426.00 RC-28
Product
Excised Patch
Recording Chamber*
Cell Biology
Material
*Note: The RC-28 is used with either P-6 or PH-6 (Heater) Platform on
page M112 and one of Stage Adapters on pages M114 and M115.
For Patch Clamps, see pages H2 to H5.
For Patch Clamp Capillary Glass,
see page M46.
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M109
Special Chambers Systems
Slice Chamber with Atmospherics RC-29
This chamber is designed for slice studies requiring the
presence of gasses in fixed proportion. It is modeled
after the RC-27L. This chamber is supplied with a slice
support. It has two ports at the top of the chamber for
the input of gasses. You must select a slice hold-down to
complete your assembly. See listing below.
Specialized Tools For Bioresearch
Specifications
RC-29
Material
Polycarbonate
Bath Dimensions:
Size, L x W
Specimen Area
Volume
38 x 18 mm
23 x 18 mm
629 µl per mm of solution height
Std. Accessories Supplied
1. SS-3 Slice Support
2. Chamber Cover
3. PE-160 Tubing (10')
4. 24 x 50 mm #1 Coverslip, pkg. of 50
Platforms Used With
1. P-6D Plain Platform
2. PH-6D Heater Platform
Catalog No.
BS4 64-0244
$
Model
403.00 RC-29
Product
RC-29 Chamber with
Slice Support*
Accessories (Spare or Replacements)
BS4 64-0273
31.00 SS-3
BS4 64-0256
35.00 SHD-27LH/10 Metal Slice Hold
Down, 1 mm
Slice Support
BS4 64-0257
35.00 SHD-27LH/15 Metal Slice HoldDown, 1.5 mm
BS4 64-0258
35.00 SHD-27LH/2
BS4 64-0259
66.00 SHD-27LP/10 Plastic Slice HoldDown, 1 mm
BS4 64-0260
66.00 SHD-27LP/15 Plastic Slice HoldDown, 1.5 mm
BS4 64-0261
66.00 SHD-27LP/2
Metal Slice HoldDown, 2 mm
Plastic Slice HoldDown, 2 mm
*Note: RC-29 Chambers require Platform P-6D or PH-6D and Microscope
Stage Adapter, see pages M112 to M115.
Slice Hold-Downs available in Kits,
see page M117.
M110
11,000 Specialty Products to Enhance Your Bioresearch
Special Chambers Systems
NEW
SIU-102 Stimulus Isolation Unit - Bipolar High Current
CC-102 Cable
SIU-102
Designed for use with Warner imaging chambers with field stimulation electrodes, the SIU102 features optical isolation at the input and transformer isolation at the output. Timing
pulses can be controlled by any device capable of generating a TTL level positive pulse – a
computer with data acquisition, for example.
Positive or negative pulses, positive or negative DC, and bipolar pulses are possible in constant current or constant voltage delivery modes. For bipolar delivery, a positive pulse during
the input pulse is followed immediately by a negative pulse of equal duration and amplitude.
Bipolar pulse widths from 100 µs to 100 seconds per each polarity are possible.
Indicator lights keep the user apprised of the following:
High voltage: Lights red when output voltage above 30 volts.
Compliance: Lights when impedance across output is too high for
current setting.
T>100 Sec:
Lights when pulse width input is longer than 100 seconds
in Bipolar mode.
Ext. Trigger: Lights when a valid input is applied to the Pulse In BNC.
Catalog No.
$
Model
BS4 64-1424
1,295.00 SIU-102
BS4 64-1425
76.00 CC-102
Product
Stimulus Isolation Unit
Cable, male banana to 1 mm jacks (for use with Warner field
stimulation chambers)
Constant Current and Constant
Voltage modes
•
Bipolar, Pulse and DC modes
•
Optical coupling is used to isolate the
stimulator from pulse source
•
Current up to 100 mA
Specifications
Output Waveform
DC, Current Pulse or
Voltage Pulse
Output Current Ranges
1, 10, and 100 mA
Output Voltage Ranges
10 and 100 V
Output Impedance
10 V Range: 1 KΩ
Voltage Mode 100 V Range: 10
KΩ
Output Compliance
100 V
Output Polarity
Selected by Mode Switch
Red connector is positive in any
positive mode Red
connector is negative in any
negative mode
Amplitude Control
Multi-turn dial
Input Pulse Requirements TTL level, positive pulse 3 volt,
1 mA minimum Minimum Pulse
Width: 100 µs Maximum Pulse
Width: 100 s (Bipolar Mode
Only)
Input Connector
BNC
Output Connector
Banana Jacks
Power Requirements
90 to 270 VAC, 50/60 Hz,
10 VA
Cell Biology
Output amplitude is set using a range mode switch in conjunction with a ten-turn potentiometer to set a percentage of the selected range.
•
Physical Size, H x W x D 8.9 x 20 x 25.4 cm
Shipping Weight
3 kg
Warranty
Two years, parts and labor
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M111
Series 20 Chamber Platforms & Stage Adaptors
Series 20 Platforms
Model P-1
Series 20 Platforms
The platform functions as a base for the chamber and provides clamping to make a seal between the
chamber and coverslip. They are machined from aluminum and finished with a black anodize. With
the exception of the model P-2 and PH-2 platforms, Series 20 platforms feature sliding hold down
clamps which permit rapid replacement of the chamber. The model P-2 and PH-2 platforms use a
one piece clamp as shown above. Sliding clamps used on most models permit easy removal of the
chamber to change the coverslip. Model P-2, see to left, uses a one piece clamp secured with 4
screws. Heater platforms are powered from a single channel of a Warner heater controller, see page
M126. Connection to the controller is made with the CC-28 cable assembly, see below.
Model PH-1
Series 20 Platforms
Aperture
Size
Plain
Model Platform
RC-22/22C/24/24E
26/26G/26GLP/26Z
17.8 mm
P-1
BS4 64-0277 304.00 PH-1
BS4 64-0284 331.00
RC-21B/21BR
RC-21BRFS
17.5 mm
P-2
BS4 64-0278
BS4 64-0285
RC-25
9.5 mm
P-3
BS4 64-0279 304.00 PH-3
BS4 64-0286 331.00
RC-25F
13.0 mm
P-4
BS4 64-0280
BS4 64-0287
RC-20/20H
12.0 mm
P-5
Specialized Tools For Bioresearch
For Chamber Model
Model P-2
$
Model Heater
PH-2
PH-4
BS4 64-0281 320.00 PH-5
BS4 64-0288 348.00
RC-27/27N/27NE/RC-28 18.9 x 35.6 mm P-6
BS4 64-0282
BS4 64-0289
RC-27L/RC-29
BS4 64-0283 320.00 PH-6D BS4 64-0290 348.00
18.9 x 35.6 mm P-6D
PH-6
Heater Platform Cable Assemblies
CC-28 Cable
Assembly
The CC-28 cable assembly is used to connect any Warner heater platform to a TC-324B or
TC-344B heater controller. The cable has two thermistors at the platform end; the control
thermistor which attaches to the platform and a bead thermistor for monitoring the bath temperature. The CC-28 is provided with a 9 pin din connector to plug into the heater controller.
An alternative cable, the CC-15, is available with no inboard connector for users who choose
to use another power source.
Heater Platform Upgrade Kit
Non-heater platforms can be converted to heater platforms with an upgrade kit consisting of
a pair of resistive heaters, heat transfer pads and mounting screws. Tapped holes for the
heaters are included in all plain platforms. The only tool required for installation is a small
Philips screwdriver. The kit is ordered with either the CC-28 or CC-15 cable assembly.
Note: Older models may not be upgradeable. Consult our technical support department.
Catalog No.
CC-15 Cable
Assembly
M112
$
$
Model
Product
BS4 64-0106
122.00 CC-28
Cable Assemblies with Connector for TC-324B/344B
BS4 64-0303
111.00 CC-15
Cable Assemblies with Tinned Leads at Connector End
BS4 64-0304
154.00 PU-28
Platform Upgrade Kit with CC-28 Cable Assembly
BS4 64-0305
143.00 PU-15
Platform Upgrade Kit with CC-15 Cable Assembly
11,000 Specialty Products to Enhance Your Bioresearch
Series 20 Chamber Platforms & Stage Adaptors
NEW
PHC Series Heater/Cooler Jackets
PHC-1
PHC-2
PHC-3
Model PHC-3 shown with mounting platform and mounted in a
Nikon stage adapter
•
Designed for Series 20
chambers
•
Optimized for the SC-20
In-line Solution
Heater/Cooler
•
•
Jackets available for
upright and inverted
microscopes
Maintains temperature
in both perfused and
static baths
RC-27 Chamber
(example)
22 X 40 mm Coverslip
Jacket Outlet tube
PHC-1 Jacket
Jacket Inlet tube
22 X 50 mm Coverslip
Platform
Clamps
Mounting Platform
Stage
Adapter
The PHC-1 is used for upright microscopes and provides a complete
thermal barrier between the chamber-forming coverslip and the
local environment. The PHC-2 and PHC-3 are designed for inverted
microscopes and provide either rectangular or round openings. (See
ordering information for chamber jacket compatibility.)
Heater/Cooler Jackets are provided with a mounting platform, which
replaces the standard platforms used with Series 20 chambers. The
platform functions as a base for the jacket /chamber sandwich and
provides the clamping pressure to make a tight seal. Mounting platforms are machined from black Delrin and are compatible with all
Series 20 stage adapters.
Cell Biology
The Warner PHC Heater/Cooler
Jackets are designed to bring
heating and cooling to our classic Series 20 Imaging and
Recording Chambers. Accurate
temperature control from 5° to
50°C can be achieved using the
PHC jackets in concert with the
SC-20 Dual In-line Solution
Heater/Cooler. Heated or chilled
water flows from the SC-20 into
the PHC jacket in direct thermal
contact with the chamber bottom coverslip.
Individual heater/cooler jackets shown without mounting platform
Assembly of Series 20 chamber with cooling jacket and stage adapter
Ordering Information
Catalog No.
$
Aperture
Size
Model
For Chamber Model
BS4 64-0354 295.00
PHC-1
RC-22/22C/24N
RC26/26G/26GLP/26Z
RC-27/27N/27NE/RC-28
RC-27L/RC-29
17.0 x 37 mm
BS4 64-0355
PHC-2
RC-22/22C/24N
RC-26/26G/26Z/26GLP
15 mm Dia.
BS4 64-0356 295.00
PHC-3
RC-27/27N/27NE
8.0 x 25 mm
U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com
M113
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