ProODO Manual
U SE R MA N UA L
Item #626279
Rev B
Drawing # A626279
December 2008
©2008 YSI Incorporated.
The YSI, ProODO, and ODO logos is a registered
trademark of YSI Incorporated.
Microsoft and Windows are registered trademarks of the Microsoft
Corporation in the United States and other countries.
Pentium is a registered trademark of Intel Corporation.
C ontents
Warranty............................................................................................................... i
Introduction........................................................................................................ 1
Getting Started.................................................................................................... 1
Initial Inspection.................................................................................. 1
Battery Installation.............................................................................. 1
Key Pad................................................................................................. 2
Initial Setup ......................................................................................... 4
Main Display........................................................................................ 6
Menu Layout......................................................................................... 6
Alpha/Numeric Entry......................................................................... 7
System ................................................................................................................. 8
Date/Time............................................................................................. 8
GLP........................................................................................................ 8
Language............................................................................................. 12
Radix Point......................................................................................... 12
Logging............................................................................................... 13
Sampling Mode.................................................................................. 13
Auto Shutoff....................................................................................... 14
Backlight............................................................................................. 14
SW Version (Software Version)........................................................ 14
Serial #................................................................................................. 14
Unit ID................................................................................................ 14
Optical Dissolved Oxygen (ODO™)................................................................ 15
ODO Probe Setup.............................................................................. 15
Calibration - Dissolved Oxygen....................................................... 18
Barometer.......................................................................................................... 23
Display - Barometer........................................................................... 23
Calibration - Barometer.................................................................... 23
Temperature....................................................................................................... 24
Taking Measurements....................................................................................... 24
Files and Site Lists............................................................................................. 25
File Memory....................................................................................... 25
View Data........................................................................................... 25
Site List................................................................................................ 26
Folder.................................................................................................. 27
Delete Data......................................................................................... 27
Data Manager Desktop Software..................................................................... 27
Using the Communications Saddle.................................................. 27
Manage Logged Data ........................................................................ 28
Real Time Data Collection................................................................ 29
Configure Instruments...................................................................... 29
Maintenance and Storage................................................................................. 30
General Maintenance - Battery Compartment Gasket................. 30
Sensor Maintenance - Dissolved Oxygen........................................ 30
Sensor Storage.................................................................................... 33
Troubleshooting................................................................................................ 34
Dissolved Oxygen Readings.............................................................. 34
Help .................................................................................................... 35
Error/Status messages . ..................................................................... 35
Restore Default Calibration Values ................................................. 36
Declaration of Conformity............................................................................... 39
Recycling............................................................................................................ 41
Contact Information......................................................................................... 42
Ordering and Technical Support...................................................... 42
Service Information........................................................................... 42
Appendix A-DO% Calibration Values............................................................ 43
Appendix B-Oxygen Solubility Table . ........................................................... 44
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Warranty
The YSI Professional ODO™ Instrument is warranted for three (3) years from date of
purchase by the end user against defects in materials and workmanship, exclusive of
batteries and any damage caused by defective batteries. ProODO™ field cable/probe
assemblies are warranted for two (2) years from date of purchase by the end user
against defects in material and workmanship. ProODO sensing caps are warranted
for one (1) year from date of purchase by the end user against defects in material
and workmanship. ProODO systems (instrument & cable/probe assemblies) are
warranted for 90 days from date of purchase by the end user against defects in
material and workmanship when purchased by rental agencies for rental purposes.
Within the warranty period, YSI will repair or replace, at its sole discretion, free of
charge, any product that YSI determines to be covered by this warranty.
To exercise this warranty, call your local YSI representative, or contact YSI Customer
Service in Yellow Springs, Ohio at +1 937 767-7241, 800-897-4151 or visit www.
YSI.com (Support tab) for a Product Return Form. Send the product and proof
of purchase, transportation prepaid, to the Authorized Service Center selected by
YSI. Repair or replacement will be made and the product returned, transportation
prepaid. Repaired or replaced products are warranted for the balance of the original
warranty period, or at least 90 days from date of repair or replacement.
LIMITATION OF WARRANTY
This Warranty does not apply to any YSI product damage or failure caused by:
1. Failure to install, operate or use the product in accordance with YSI’s written
instructions;
2. Abuse or misuse of the product;
3. Failure to maintain the product in accordance with YSI’s written instructions
or standard industry procedure;
4. Any improper repairs to the product;
5. Use by you of defective or improper components or parts in servicing or
repairing the product;
6. Modification of the product in any way not expressly authorized by YSI.
THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR
IMPLIED, INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS
FOR A PARTICULAR PURPOSE. YSI’s LIABILITY UNDER THIS WARRANTY
IS LIMITED TO REPAIR OR REPLACEMENT OF THE PRODUCT, AND THIS
SHALL BE YOUR SOLE AND EXCLUSIVE REMEDY FOR ANY DEFECTIVE
PRODUCT COVERED BY THIS WARRANTY. IN NO EVENT SHALL YSI BE
LIABLE FOR ANY SPECIAL, INDIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES RESULTING FROM ANY DEFECTIVE PRODUCT COVERED BY
THIS WARRANTY.
i
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ii
I ntroduction
Thank you for purchasing the YSI Professional Optical Dissolved Oxygen (Pro
ODO) instrument. The YSI ProODO™ measures dissolved oxygen in water using
lifetime luminescence technology and uses a digital signal to send information
between the instrument and probe. Key advantages of the new ProODO include
the elimination of sensor flow dependence and sensor warm-up time, greater
stability, the ability to zero the sensor for more accurate measurements at low
dissolved oxygen levels, and the elimination of frequent membrane/electrolyte
changes. The ProODO also features a waterproof (IP-67) case, a rugged MS-8
cable connector, backlit display and keypad, user-selectable cable lengths, USB
connectivity, large memory with extensive site list capabilities, and a rugged,
rubber over-molded case. For product specification information, please visit
www.ysi.com or contact Technical Support at 800-897-4151 (+1 937 767-7241).
Reading the entire manual before use is recommended for an overall
understanding of the instrument’s features.
G etting S tarted
Initial Inspection
Carefully unpack the instrument and accessories and inspect for damage.
Compare received parts with items on the packing list. If any parts or materials
are damaged or missing, contact YSI Customer Service at 800-897-4151 (+1 937
767-7241) or the authorized YSI distributor from whom the instrument was
purchased.
Battery Installation
The ProODO uses 2 alkaline C-cell batteries. Battery life depends on sampling
mode and usage. When used in Manual Sampling mode, under normal
conditions, battery life is approximately 80 hours at room temperature. The
use of Automatic Sampling mode may slightly reduce the battery life. See the
System section of this manual for more information on Sampling Modes.
Figure 1. ProODO with battery cover removed. Notice battery symbols indicating
polarities.
1
To install or replace the batteries:
1. Turn the instrument over to view the battery cover on the back.
2. Unscrew the four captive battery cover screws.
3. Remove the battery cover and install the new batteries, ensuring correct
polarity alignment on the instrument or the removed cover (Figure 1).
4. Replace the battery cover on the back of the instrument and tighten the four
screws. Do NOT over-tighten.
Key Pad
2
System
3
Probe Calibration File
1
4
13
12
5
11
6
10
7
9
8
Figure 2
Number
Key
Description
1
System
Opens System Menu from any screen.
Use to adjust system settings.
2
Probe
Opens Probe Menu from any screen.
Use to setup DO probe, sensor
cap, and display units.
3
Calibrate
Opens Calibrate Menu from any screen.
Use to calibrate dissolved oxygen.
2
Number
Key
Description
4
File
Opens File Menu from any screen.
Use to view data and GLP files, set up
site and folder lists, and delete data.
5
Backlight
Press to turn the instrument backlight on and
off and to adjust the display contrast when
pressed with the left or right arrow key.
6
Right Arrow
Use to navigate right in alpha/numeric entry
screens. Can be pressed simultaneously with
Backlight button to increase display contrast.
7
Down Arrow
Use to navigate through menus and to navigate
down in alpha/numeric entry screens.
8
Power
Press and hold for 2 seconds to
turn the instrument on.
Press to turn off.
9
Help
Press to receive hints & tips during operation.
10
Enter
Press to confirm selections, including
alpha/numeric key selections.
11
Left Arrow
Use to navigate left in alpha/
numeric entry screens.
Press to return to previous menu in all
screens except alpha/numeric entry.
Can be pressed simultaneously with Backlight
button to decrease display contrast.
12
Exit/Escape
Exits back to Run Screen.
When in alpha/numeric entry screen,
escapes to previous menu.
13
Up Arrow
Use to navigate through menus and to
navigate up in alpha/numeric entry screens
3
Initial Setup
Throughout the manual, the term “probe” refers to the end of the cable where
the sensor is located, the term “sensor” refers to the Optical Dissolved Oxygen
sensing portion of the cable/probe assembly, and the term “sensor cap” refers to
the removable sensing cap that is replaced about once per year (Figure 3).
Sensor Cap
Sensor
Probe
Figure 3
Unpacking the ODO Probe
i
Each ProODO cable/probe assembly and replacement ODO
sensor cap includes an instruction sheet with important
information unique and specific to each individual sensing cap.
Remove the cable/probe assembly from the shipping container and locate
the instruction sheet included with your assembly. This instruction sheet is
important because it includes the calibration coefficients for your sensor cap.
After using this sheet for general probe setup, be sure to store it in a safe place in
case you need to reload these calibration coefficients in the unlikely event that
they are ever deleted from the probe.
i
Note - A new cable/probe assembly already has a
sensor cap installed and the sensor cap coefficients
are preloaded into the probe at the factory.
Preparing the probe for the first time:
1. Remove the metal probe guard from the probe by turning it
counterclockwise.
2. Remove the red storage cap which contains a moist sponge from the end of
the probe by pulling it straight off the sensor. Save this to use later for long
term storage.
4
3. Reinstall the probe guard by sliding it carefully over the sensor and then
threading it onto the cable/probe assembly with a clockwise rotation (Figure
4).
4. Locate the grey calibration/storage sleeve that was shipped with your probe/
cable assembly. Moisten the sponge in the grey calibration/storage with a
small amount of clean water.
5. Slide the calibration/storage sleeve over the probe guard to keep the probe
in a moist atmosphere for storage or calibration (Figure 5). It is important
to always keep your sensor in a moist environment so the sensor cap does
not dry out. (See Care, Maintenance, and Storage more information.)
Figure 4
Connecting
Instrument
Figure 5
the
Probe/Cable
Assembly
to
the
To connect the cable, align the keys on the cable connector to the slots on the
instrument connector. Push together firmly, then twist the outer ring until it
locks into place (Figure 6). This MS-8 (Military Spec) connection is waterproof.
Figure 6. Note the keyed connector. The cable and instrument
connectors can only be mated once the keyed sections are properly
aligned. When disconnected, the cable connector and the
connector on the instrument maintain an IP-67 rating.
5
Main Display
Press and hold the Power key
for two seconds to turn the instrument
on. The instrument will briefly display to splash screens then go directly
to the main run mode screen. The first time the instrument is powered
up, you will need to set the date and time. Follow the instructions under
the Setup | System | Date/Time section of this manual.
The display at the left shows the run mode
(main display) with temperature in °C,
barometer in mmHg, DO in % and mg/L as
the reported parameters. The date, time and
battery level are indicated at the bottom of
the screen. The logging preference of Log
One Sample at a time is indicated at the top
of the screen.
This screen also shows the message line
towards the bottom of the display above the
date and time. In this case, it doesn’t show
a message. However, messages will appear
frequently to indicate successful calibrations,
saved configuration changes, etc.
will show up on
A USB symbol
the bottom of the display when connected
through USB with the communications saddle. The instrument will display full
battery power when it is receiving power through the USB connection.
i
Contrast – the contrast adjustment can be accomplished
by repeatedly pressing the backlight key and the
left or right arrow key at the same time.
Menu Layout
Press Esc
at anytime in the menus to escape back to the Run screen. The
can be used to go back to the previous menu in all screens
left arrow
except alpha/numeric entry screens. You must use Esc to get out of the alpha/
numeric screens if you want to exit before finishing or without saving changes.
Functions that are enabled appear as a circle with a dot . Disabled functions
appear as a circle only . In addition, some options appear as an empty box P
or a box with a check mark Q.
6
Alpha/Numeric Entry
The numeric screens will display numbers only (shown above, left). Alpha/
numeric screens will display numbers across the top and letters along the
bottom rows (shown above, right). Letters appear as a common keyboard
arrangement.
When an alpha or numeric character is required, the display will show the alpha/
numeric entry screen. To select a character, highlight it by using the arrows to
move the highlight box over the desired selection. Then, press Enter on the
keypad to confirm the selection. After confirming the selection, it will appear
in the line at the top of the display.
For capital letters or lower case entry, highlight “SHIFT” and press Enter on the
keypad to change the characters from upper to lower case.
To delete the entire line of the current entry, highlight ) and press Enter on the
keypad. The ! symbol functions as a backspace key in the alpha/numeric entry
screens by deleting one character at a time. Use the “SPACE” function to add a
space between characters.
When you have finished entering the correct information (16 character max),
highlight <<<ENTER>>> at the bottom of the screen and press Enter on the
keypad to confirm.
i
The
key cannot be used to escape to the previous menu from
an alpha/numeric entry screen. Instead, use the
key to go
back to the previous menu when in alpha/numeric entry screens.
7
S ystem
Press System
to access any of the following menu items.
The System menu will allow you to access the
setup options of the instrument including;
Date/Time, GLP, Language, Radix Point,
Logging, Sampling, Auto Shutoff, Backlight,
Sw (Software) Version, Serial #, and Unit ID.
Any item with [brackets] shows the current
setting inside the brackets. For instance, in the
example at the left, Radix Point is currently set
to [Decimal].
Date/Time
Highlight Date/Time from the System menu. Press enter to select.
Date Format – Highlight and press enter to open a sub menu for selecting
the preferred date format: YY/MM/DD, MM/DD/YY, DD/MM/YY, or
YY/DD/MM.
Date – Highlight and press enter to use the numeric entry screen to set the
correct date.
Time Format – Highlight and press enter to open a submenu to select the
preferred time format from 12-hour or 24-hour.
Time – highlight and press enter to use the numeric entry screen to set the
correct time.
GLP
The GLP or ‘Good Laboratory Practice’ file saves detailed information
about calibrations. It also includes diagnostic information about the sensors.
Calibrations are logged into a file, the GLP, for later review as needed. A single
GLP file is utilized to store all calibration records and is capable of storing 500
8
records. Once the GLP file is full, the instrument will begin to overwrite the
oldest record with each new calibration record.
i
In order to keep all of your GLP records, periodically download
the GLP to Data Manager and export it to another program.
Otherwise, the instrument will overwrite the oldest record
once the memory is full. Also, since Data Manager saves
GLP files under the Unit ID, you must periodically export
and rename the GLP file on your PC or it will be overwritten
each time you upload the GLP file from the instrument.
Several calibration parameters are saved for each calibration including optional
ones that can be enabled by the user. Standard parameters include date/time
stamp, calibration method, and sensor information. Optional, user selectable
parameters include User ID, Probe ID, and User Fields 1 and 2.
In addition, there will be information specific to the Dissolved Oxygen or
Barometer calibration record. A Dissolved Oxygen calibration record will
contain the following calibration specific parameters:
DO
Sensor - Serial Number of the Sensor
Calibration Method - Zero, %, or mg/L
Cal Value
Sensor Value - Tangent of the angle between reference and signal LED, must be within 0.2 of the default value in the sensor, typically between
-0.53 and -2.17
Salinity Mode - Always manual
Salinity Value - Value entered by the user
Barometer - Barometric Pressure during calibration
Temperature - Temperature during calibration
Calibrate Status - Calibrated
A Barometer calibration record will contain the following calibration specific
parameters:
Barometer
Barometer - Calibrate value in kPa
Calibrate Status - Calibrated
9
An example of a GLP record
(Operation Performed is % DO Calibration)
*** Calibrate – DO ***
Date: 11/01/2008 MM/DD/YY
Time:
04:03:05 PM
User ID:
Tech0001
Probe ID:
SN: 08D
User Field #1
Cloudy
Sensor: 08F000015
Method: DO Air Calibrate
Cal Value: 100.0 %
Sensor Value:
-0.591150
Salinity Mode:
Manual
Salinity Value:
0.000000 SAL PSU
Barometer
767.09 mmHg
Temperature
19.2 °C
Calibrate Status:
Calibrated
___________________________
In the System menu, highlight GLP and press
enter to view and modify the GLP settings.
Highlight Options and press enter to access
User ID, Probe ID, User Defined Fields, and
Re-Cal Prompt.
User ID may be used to identify the person
calibrating the instrument. Highlight User ID
and press enter to select or edit a User ID from
a list of previously entered IDs. Or, highlight
Add New and press enter to create a new User
ID using the alpha/numeric entry screen. The
User ID may also be changed in the Calibration
menu during the calibration process. The
selected User ID will be stored in the GLP file
with each calibration record. A User ID could
be a person’s initials or badge number. The
character limit is 16 characters.
Probe ID is stored with the calibration record
and may be used to distinguish one cable/
10
probe assembly from another, typically by serial number. Highlight Include
Probe ID and press enter to turn this function on and off. There will be a check
mark in the box when the function is enabled. Highlight Probe ID and press
enter to add, view, edit, or select a Probe ID. Probe ID may also be changed in
the Calibration menu during the calibration process. The character limit is 16
characters.
User Fields 1 and 2 are stored with the calibration record and may be used
to enter other parameters pertinent to the user, such as weather conditions,
elevation, etc. Highlight Include User Field 1 or Include User Field 2 and press
enter to turn this function on and off. Highlight User Field 1 or User Field 2
and press enter to add, view, edit, or select a User Field. The character limit is
16 characters. When enabled, a prompt for selecting a User Defined Field will
appear during the calibration process.
Re-Cal Prompt may be used to remind the user
to perform a calibration. To set a time interval,
highlight DO and press enter to access the
numeric entry screen. Enter a value in days and
press enter to confirm the reminder time. To
turn off the Re-cal prompt, set the reminder to
zero (0) days (this is the default).
The Security section of the GLP menu is a password protected area. This area
includes options to set a new password and to lock access to the calibration
menu. When first viewing the security menu, you will be required to enter a
password. Use the “shift” on the alpha/numeric screen to switch to lower case
and enter “ysi123”. This is the default password.
Protect Cal can be enabled or disabled. When enabled, the user must know and
enter the instrument’s password to enter the calibration menu option. Highlight
Protect Cal and press enter to enable (Q) or disable (P) this feature.
Set Password allows a user to set the security password. Highlight Set Password
and press enter. Using the alpha/numeric entry screen, enter the new password.
The password can have up to 16 characters.
Contact YSI Technical Support at environmental@ysi.com or +1 937 767-7241 if
you forget or misplace your password.
i
Once a password is set, and the GLP security screen
exited, a password must be entered to make changes
under GLP security. Keep passwords in a safe place.
11
Language
To change the instrument’s language setting,
highlight Language in the System menu and
press Enter. Highlight the desired language
and press enter to confirm. Languages include
English, Spanish, French, German, Italian,
Portuguese, and Norwegian.
Radix Point
Radix Point allows the user to select between
a comma or a decimal in numeric radix point
for displayed values. For example, 1.00 becomes
1,00 when Use Comma is selected. Highlight
Use Decimal or Use Comma and press enter to
make your selection.
12
Logging
Highlight Logging and press enter to change the logging setting in the instrument.
Logging Settings include Use Site List, Use Folder List, Continuous Mode, and
Interval.
Use Site List and Use Folder List are optional
ways of filing or ‘tagging’ your logged data
points. If these settings are enabled, you will
be prompted to select a Site and/or Folder to
‘tag’ to logged data points. See the Taking
Measurements section of this manual for
information on creating Site and Folder Lists.
Check the box for Continuous Mode if you
want to log samples continuously at a specific
time interval. To set the length of time between
logged samples, highlight Interval and press
Enter. Enter the interval as HH:MM:SS. This
interval will display at the top of the screen
when you select the Start Logging option in
run mode. If operating the unit in Manual
mode, see next section, the continuous logging
interval must be set to 10 seconds or greater in order to log data.
To log one sample at a time, uncheck Continuous Mode.
Sampling Mode
The Sampling mode can be set to Automatic or Manual. In Automatic mode,
which is the default mode, the readings are
constantly updated. In Manual mode, the
readings are held or ‘locked’ on the display
once they reach the stability of the Auto Stable
setting in the Probe menu. The user must then
press a key to either log the held data or update
the measurements. Selecting Manual mode may
increase the instrument’s battery life. Highlight
either Automatic or Manual and press enter to
make your selection. If continuously logging in
Manual mode, the logging interval must be set
to 10 seconds or greater.
13
Auto Shutoff
Auto Shutoff powers the instrument off after a user specified time period.
Highlight Auto Shutoff and press enter. Using the numeric entry screen, enter
a value between 0 and 360 minutes. To disable auto shutoff, set the value to 0
(zero).
Backlight
Backlight can be set to Automatic or Manual. Automatic turns the backlight on
when you turn the instrument on and when you
press any key. Manual allows you to turn the
backlight on or off with the backlight key
.
When in Automatic mode, the instrument will
turn the backlight off after 60 seconds without
any keys being pressed. If a key is pressed
during that time, the instrument will “reset” the
60 second time period. The lighted keypad will
turn off after approximately 20 seconds.
SW Version (Software Version)
SW Version shows the instrument’s software version.
Serial #
Serial # shows the instrument’s serial number and allows you to match it with
the number engraved on the back of the instrument’s case. The Serial # is also
the default Unit ID.
Unit ID
Unit ID is used to identify instruments in the Data Manager software included
with your instrument. It is also used to identify GLP files, Site Lists, Configuration
Files, and Data files transferred from the instrument to the PC. The default Unit
ID is the Instrument’s serial number. To modify the Unit ID, highlight Unit ID,
press enter and use the alpha/numeric entry screen. The character limit is 16
characters.
14
O ptical D issolved O xygen
(OD O™)
i
Each ProODO cable/probe assembly and replacement ODO
sensor cap includes an instruction sheet with important
information unique and specific to each individual sensing
cap. This instruction sheet is important because it includes
the calibration coefficients for the sensor cap. After using this
sheet for general probe setup, be sure to store it in a safe place
in case you need to reload these calibration coefficients in the
unlikely event that they are ever deleted from the probe.
See Initial Setup | Unpacking the ODO Probe for initial setup instructions.
i
It is important to always keep your sensor in a moist
environment so the sensor cap does not dry out. (See
Maintenance and Storage for more information.)
ODO Probe Setup
Press Probe
, Highlight DO, press enter.
Enabled allows you to enable or disable the DO
sensor. Highlight Enabled and press enter to
activate or deactivate dissolved oxygen.
The DO Setup menu also displays the ODO
probe’s SW Version and Serial #. This
information is programmed into the probe at
the factory and digitally sent to the instrument
when the cable is connected.
Local DO allows for localized DO%
measurements. This sets the calibration value
to 100% regardless of the altitude or barometric
pressure. Highlight Local DO and press enter
to enable or disable this function. Local DO
is a method for the ProODO to factor in the
barometric pressure on each DO measurement.
In essence, even if the barometric pressure changes you wouldn’t notice the
difference with the DO% readings in air-saturated water or water-saturated air.
15
Local DO is ideal for EU compliance. When Local DO is enabled, an L will
appear next to DO% on the run screen. DO mg/L readings are unaffected by the
selection of DO Local.
LDS (Last Digit Suppression) rounds the DO value to the nearest tenth; i.e. 8.27
mg/L becomes 8.3 mg/L. Highlight LDS and press enter to activate or deactivate
LDS. Some users may not want to read out to the hundredths place since the DO
mg/L accuracy spec is to the tenths place.
Sensor Cap allows you to view and enter information specific to the Sensor
Cap installed on the probe. Highlight Sensor Cap and press enter to view the
Sensor Cap’s Serial #, Temperature Coefficient, and Sensor Cap Coefficients.
This information is programmed into the sensor at the factory and sent to the
instrument when the cable is connected. Since the Temperature Coefficient is
programmed into the sensor at the factory, it should not be modified unless
instructed by YSI Technical Support.
The Sensor Cap Coefficients need to be updated when the Sensor Cap is
replaced. The Sensor Cap should be replaced about once per year. See the
Dissolved Oxygen Sensor Maintenance section of this manual or the instruction
sheet included with replacement Sensor Cap for instructions on updating the
Sensor Cap Coefficients. When updating the Coefficients, the Sensor Cap Serial
# will be updated automatically based on your entries.
Display - Dissolved Oxygen
Press Probe
, highlight Display and press enter. Highlight DO and press
enter. Note, you will not be able to display dissolved oxygen unless it is Enabled
in the DO Setup menu first, see previous section.
DO % will show DO readings in a percent scale from 0
to 500%.
DO mg/L will show DO readings in milligrams per liter
(equivalent to ppm) on a scale from 0 to 50 mg/L.
DO ppm will show DO readings in parts per million (equivalent to mg/L) on a
scale from 0 to 50 ppm.
All units can be displayed simultaneously on the screen. Highlight the desired
unit(s) and press enter to select. A check mark in the box next to the unit will
indicate that it is enabled.
16
Auto Stable - Dissolved Oxygen
Press Probe
enter.
, highlight Auto Stable and press enter. Highlight DO and press
Auto Stable indicates when a reading is stable.
Highlight Enabled and/or Audio Enabled
(instrument will beep when the stability is
achieved) and press enter to confirm. When
Auto Stable is enabled, AS will blink next to the
parameter until it is stable. Once the parameter
is stable, AS will stop blinking.
The Auto Stable Sensitivity can be decreased or
increased. Highlight Sensitivity and use the left and right arrow keys to slide
the bar. The more sensitive you make it (larger black bar) the harder it is to
achieve stability in a changing environment.
The Auto Stable system works by examining the previous 5 readings, computing
the percent change in the data and comparing that change against a % threshold
value. The % threshold value is determined by the Sensitivity bar setting. The
following chart can be used as a guide when setting the Sensitivity bar.
Sensitivity selected by User
% Data Variance Threshold
100 - Most Sensitive, Sensitivity
bar is set to the far right
0.05%
75
0.62525%
50
1.275%
25
1.8875%
0 - Least Sensitive, Sensitivity
bar is set to the far left
2.5%
Example:
The instrument obtained the following data:
Reading #1
Reading #2
Reading #3
Reading #4
Reading #5
95.5 DO%
95.7 DO%
95.8 DO%
96.1 DO%
95.3 DO%
17
The instrument is programmed to determine the minimum and maximum
data value over the previous 5 samples, and to compute the percent difference
between those values. In this example, that gives a percent change of:
% Change = 100 * ((96.1 – 95.3 ) / 95.3)
% Change = 0.83%
In this example, if the Sensitivity Bar is set to the far right, the Auto Stable
requirement would not be met and AS would continue to blink. However,
if the sensitivity bar is set to the median threshold (1.275%), the Auto Stable
requirement would be met and AS would display steadily on the display.
If the Manual Sampling mode in the System menu is enabled, the Auto Stable
function will automatically be enabled and the sensitivity setting will be used to
determine when to hold the readings on the display. See Sampling Mode in the
System section of this manual for more information on the two Sampling mode
options.
Salinity Correction
The last feature in the Probe menu is the Salinity correction value for the mg/L
readings. Press Probe
, highlight Salinity, and press enter. Then, use the
numeric entry screen to enter the Salinity value of the water you will be testing
from 0 to 70 ppt.
The value entered here will be used when calculating mg/L from the temperature
and % saturation readings. As the salinity of water increases, its ability to dissolve
oxygen decreases. For example, fully oxygenated 20 °C water at sea level with
zero salinity will hold 9.092 mg/L of dissolved oxygen. If that same sample had
a salinity value of 9 ppt, then it would hold 8.621 mg/L of dissolved oxygen.
Therefore, to obtain accurate mg/L readings, it is important that you know the
salinity of the water you will be testing and input the value into the instrument.
The salinity of fresh water is typically 0-0.5 ppt and seawater is typically 35 ppt.
You will also have the opportunity to enter or modify the Salinity correction
value during DO calibration. Appendix B shows the oxygen solubility table.
Calibration - Dissolved Oxygen
The ProODO sensor is an optical luminescent sensor which has greater stability
and is less susceptible to calibration drift than traditional electrochemistry
sensors. This increased stability means that the instrument may hold its
calibration for many months. However, for the highest data accuracy, YSI
recommends verifying the instrument’s calibration on a daily basis. To verify
the instrument’s calibration, place the sensor in its calibration environment
and check to see that the DO% is reading its calibration value based on the
18
barometric pressure. Refer to Appendix A for the DO% calibration values based
on barometric pressure.
The ProODO offers several options for calibrating dissolved oxygen. The
first and second methods calibrate the DO % saturation value to either water
saturated air or air saturated water. The third and fourth calibrates in mg/L
or ppm to a solution with a known DO concentration (usually determined by a
Winkler Titration). The fifth option is a zero calibration. If performing a zero
calibration, you must also perform a %, mg/L, or ppm calibration following the
zero calibration. For both ease of use and accuracy, YSI recommends performing
the following DO % water saturated air calibration:
i
It is not necessary to calibrate in both % and mg/L or ppm.
Calibrating in % will simultaneously calibrate
mg/L and ppm and vice versa.
Calibrating DO %
1-Point Calibration
in
Water
Saturated
Air:
Moisten the sponge in the storage sleeve with a small amount of clean water.
The sponge should be clean since bacterial growth may consume oxygen and
interfere with the calibration. Make sure there are no water droplets on the
sensor cap and temperature sensor and then install the storage sleeve over the
probe. Make sure the DO and temperature sensors are not immersed in water.
The storage sleeve ensures venting to the atmosphere. Wait approximately 5
to 10 minutes for the storage container to become completely saturated and to
allow the temperature and dissolved oxygen sensors to stabilize.
Press Calibration
. If Probe ID or User ID
are enabled in the System GLP menu you will be
able to highlight these features and add, select,
edit, or delete an ID. When enabled these IDs
are stored with each calibration record in the
GLP file.
After selecting the Probe ID and/or User ID if
appropriate, highlight DO and press enter.
Highlight DO % and press enter to confirm.
The instrument will use the value from the
internal barometer during calibration and will
display this value in brackets at the top of the
19
display. Highlight the barometer value and press
enter to adjust it if needed. If the barometer
reading is incorrect, it is recommended that
you calibrate your barometer. Note - the
barometer should be reading “true” barometric
pressure (see Barometer section for more
information on “true” barometric pressure).
If the value is acceptable, there is no need to
change it or perform a barometer calibration.
Wait for the temperature and DO% values
under “Actual Readings” to stabilize, then highlight Accept Calibration and
press enter to calibrate. If User Field 1 or 2 are enabled, you will be prompted to
select the fields and then press Cal
to complete the calibration. The message
line at the bottom of the screen will display “Calibrating Channel...” followed by
“Calibration Successful”. Press Esc
Calibrating DO %
1-Point Calibration
to cancel the calibration.
in
Air
Saturated
Water:
Continuously sparge water with air using an air stone or some type of aerator
until the water becomes completely saturated with air. For 500 ml of water at
room temperature, it may take 60 minutes or more for the water to become
completely saturated. Place the sensor in the air saturated water, be sure to
immerse both the ODO and Temperature sensor into the water, and wait for the
readings to stabilize (at least 2 minutes).
Press Calibration
. If Probe ID or User ID are enabled in the System GLP
menu you will be able to highlight these features and add, select, edit, or delete
an ID. When enabled these IDs are stored with each calibration record in the
GLP file.
After selecting the Probe ID and/or User ID if appropriate, highlight DO and
press enter. Highlight DO % and press enter to confirm.
The instrument will use the value from the internal barometer and will display
this value in brackets at the top of the display. Highlight the barometer value
and press enter to adjust it if needed. If the barometer reading is incorrect,
it is recommended that you calibrate your barometer. Note - the barometer
should be reading “true” barometric pressure (see Barometer section for more
information on “true” barometric pressure). If the value is acceptable, there is
no need to change it or perform a barometer calibration.
20
Wait for the temperature and DO% values under “Actual Readings” to stabilize,
then highlight Accept Calibration and press enter to calibrate. If User Field 1
or 2 are enabled, you will be prompted to select the fields and then press Cal
to complete the calibration. The message line at the bottom of the screen will
display “Calibrating Channel...” followed by “Calibration Successful”. Press Esc
to cancel the calibration.
Calibrating in Air Saturation Percent (DO %):
2-Point Calibration with Zero Solution
Place the sensor in a solution of zero DO. Be sure to immerse both the ODO and
Temperature sensor into the water.
A zero DO solution can be made by dissolving approximately 8 - 10 grams of
sodium sulfite (Na2SO3) into 500 mL tap water or DI water. Mix the solution
thoroughly. It may take the solution 60 minutes to be oxygen-free.
Press Calibration
. If Probe ID or User ID are enabled in the System GLP
menu you will be able to highlight these features and add, select, edit, or delete
an ID. When enabled these IDs are stored with each calibration record in the
GLP file.
After selecting the Probe ID and/or User ID if appropriate, highlight DO and
press enter. Highlight Zero and press enter. Wait for the temperature and
DO% values under “Actual Readings” to stabilize, then press enter to Accept
Calibration. If User Field 1 or 2 are enabled, you will be prompted to select the
fields and then press Cal
to complete the calibration. The screen will then
prompt for a follow-up second point calibration.
Highlight DO% for the second calibration point and press enter to continue with
the next point at full saturation. Rinse the sensor of any zero oxygen solution
using clean water. Follow the steps under either of the DO % Saturation methods
previously discussed to complete the second point.
Calibrating in MG/L
1-Point Calibration
or
PPM
as
a
Titration:
Place the sensor into a water sample that has been titrated by the Winkler method
to determine DO concentration. Allow the sensor to stabilize.
Press Calibration
. If Probe ID or User ID are enabled in the System GLP
menu you will be able to highlight these features and add, select, edit, or delete
21
an ID. When enabled these IDs are stored with each calibration record in the
GLP file.
After selecting the Probe ID and/or User ID if appropriate, highlight DO and
press enter. Highlight DO mg/L or ppm and press enter.
Highlight Calibration value and press enter to
manually input the sample’s dissolved oxygen
value. Highlight Accept Calibration and press
enter once the temperature and Dissolved
Oxygen readings have stabilized. If User Field 1
or 2 are enabled, you will be prompted to select
the fields and then press Cal
to complete the
calibration. After completing the calibration, the
message line will display “Calibration Successful”.
Press Esc
to cancel the calibration.
Calibrating in MG/L or PPM as a
2-Point Calibration with Zero Solution
Titration:
Place the sensor in a solution of zero DO. Be sure to immerse both the ODO and
Temperature sensor into the water.
A zero DO solution can be made by dissolving approximately 8 - 10 grams of
sodium sulfite (Na2SO3) into 500 mL tap water. Mix the solution thoroughly. It
may take the solution 60 minutes to be oxygen-free.
Press Calibration
. If Probe ID or User ID are enabled in the System GLP
menu you will be able to highlight these features and add, select, edit, or delete
an ID. When enabled these IDs are stored with each calibration record in the
GLP file.
After selecting the Probe ID and/or User ID if appropriate, highlight DO and
press enter. Highlight Zero and press enter. Wait for the temperature and
DO% values under “Actual Readings” to stabilize, then press enter to Accept
Calibration. If User Field 1 or 2 are enabled, you will be prompted to select the
fields and then Press Cal
to complete the calibration. The screen will then
prompt for a follow-up second point calibration.
Highlight the desired calibration units (mg/L or ppm) and press enter to
continue with the next point in a known titrated value. Rinse the sensor of
any zero oxygen solution using clean water. Follow the steps under One Point
Calibration for the mg/L or ppm for the second point.
22
B arometer
All ProODO instruments contain an internal barometer.
Display - Barometer
Press Probe
, highlight Display, and press enter. Highlight Barometer, press
enter. The measurement unit options are: mmHg, inHg, mBar, PSI, kPa, or Atm.
Only one unit can be displayed at a time. Select None if you do not want to
display a barometric pressure reading.
Whether or not you choose to display the barometer reading, the barometric
pressure will still be used for calibrating DO and for compensating for pressure
changes if Local DO is enabled.
Calibration - Barometer
The barometer in the instrument is calibrated at the factory. If the barometer
requires calibration, press Calibrate
. If Probe ID or User ID are enabled in
the System GLP menu you will be able to highlight these features and add, select,
edit, or delete an ID. When enabled these IDs are stored with each calibration
record in the GLP file.
After selecting the Probe ID and/or User ID if appropriate, highlight barometer
and press enter.
Highlight the desired unit and press enter.
Highlight Calibration Value and press enter to
manually enter the correct “true” barometric
pressure. Next, highlight Accept Calibration,
and press enter. If User Field 1 or 2 are enabled,
you will be prompted to select the fields and then
press Cal
to complete the calibration. After
completing the calibration, the message line will
display “Calibration Successful”. Press Esc
to cancel the calibration.
23
i
Laboratory barometer readings are usually “true”
(uncorrected) values of air pressure and can be used “as is”
for barometer calibration. Weather service readings are
usually not “true”, i.e., they are corrected to sea level, and
therefore cannot be used until they are “uncorrected”. An
approximate formula for this “uncorrection” is below:
True BP = [Corrected BP] – [2.5 * (Local Altitude in ft. above sea level/100)]
T emperature
All probe/cable assemblies have built-in temperature. Temperature calibration
is not required nor is it available. To set the units, press Probe
, and select
Display. Highlight Temperature and press enter. Highlight the desired
temperature units of °F, °C, or K and press enter to confirm the selection. Only
one temperature unit may be displayed at a time. You may also choose to not
display temperature. If you choose not to display temperature, dissolved oxygen
readings will still be temperature compensated.
T aking M easurements
1. To take readings, insert the probe into the sample. Move the probe in the
sample to release any air bubbles and to provide a fresh sample to the sensor
cap. This movement is only necessary when first inserting the probe into
the sample. Since the ProODO utilizes optical luminescent technology,
continuous sample movement or stirring is not required. The probe will
fit into a 300 mL BOD bottle for taking initial and final BOD readings. For
best results in a BOD bottle, a stirring device should be used to properly mix
the sample and to keep solids from settling at the bottom.
2. Allow the temperature readings to stabilize and wait approximately 25-35
seconds for the DO readings to stabilize.
3. Log One Sample is already highlighted in Run mode. Press Enter to open
a submenu. Highlight Sites or Folders and press Enter to select the site or
folder to log the sample to.
4. If necessary, use the keypad to create a new Site or Folder name. If Site List
and Folder List are disabled in the System menu, you will not see these
options when logging a sample.
24
5. Once the Site and/or Folder name is selected, highlight Log Now and press
Enter. The instrument will confirm that the data point was successfully
logged.
If you would like to log at a specific interval vs. logging one sample at a time
, then highlight Logging and press Enter. Select
or vice versa, press System
Continuous Mode and adjust the time Interval if necessary. On the Run screen,
the option to log will change from Log One Sample to Start Logging based on the
time interval entered in the Logging Menu. Note – When utilizing the Manual
sampling mode, the Continuous Logging Interval must be set to 10 seconds or
greater.
During a continuous log, the Start Logging dialog box on the Run screen will
change to Stop Logging. Press Enter to stop continuous logging.
Note - There is NO WARM-UP period associated with the ProODO sensor so
you may wish to turn off the ProODO instrument between readings to conserve
battery power.
F iles and S ite L ists
File Memory
To view the file memory, press File
.
The Data Memory shows a percentage
indicating the amount of memory available.
If the file memory is near 0%, files should be
downloaded to a PC and/or deleted to free up
memory.
View Data
Press File
, highlight View Data, press enter.
Configuring your data view:
Site: will allow you to view data from one
particular site or all sites. Highlight Site, press
enter, and select the site you wish to view data
from or select All Sites to view data from all
sites.
25
Folder: will allow you to view data from one particular folder or all folders.
Highlight Folder, press enter, and select the file you wish to view data from or
select All Folders to view data from all folders.
Begin and End will allow you to view data that was collected between two dates.
Highlight Begin and/or End, press enter, and use the numeric entry screen to
select the dates you wish to view.
After making your selections in the Data Filter,
highlight Show Data and press enter. The data
will have date and time stamps. You will likely
have to scroll up and down and side to side
using the arrow keys to completely view the
data file.
Site List
To modify the Site List, press File
, highlight
Site List, and press enter. Enter new site names
or edit existing sites with the alpha/numeric
entry screen. Site lists can also be created and
edited on your PC with Data Manager and then
downloaded to the instrument.
26
Folder
To modify the Folder List, press File
, highlight Folder List, and press enter.
Enter new Folder names or edit existing folders with the alpha/numeric entry
screen.
Delete Data
Press File
, highlight Delete Data, and press enter. Enter the criteria for the
data you wish to delete in the Delete Data Filter, then highlight Delete Data and
press enter.
D ata M anager
D esktop S oftware
Data Manager is provided with the purchase of a ProODO Instrument. Data
Manager is a powerful Windows® based software that will allow you to easily
manage logged data, set up instruments, and conduct real time studies.
Data Manager needs to be installed on a PC before use. Once the communications
saddle is connected to an instrument and the PC, the Data Manager software will
recognize the attached instruments. Data Manager will identify the connected
instruments by their Unit ID.
From the ‘home’ screen of Data Manager, you can select one of the following
functions: Retrieve Instrument Data, Real Time Instrument Data, Instrument
Configuration, or View Saved File/Data.
Using the
Communications Saddle
u
WARNING: DO NOT connect the Communications Saddle to
your PC before installing Data Manager. The Communication
Saddle drivers must be installed prior to connecting it to your
PC. The drivers will install automatically during the Data
Manager installation. The first time the saddle is connected to
the PC, you may have to walk through a couple of installation
wizards. For detailed instruction, please refer to the Readme file
located on the CD that was included with your instrument.
27
A PC will recognize the Communications Saddle (saddle) as a YSI water quality
instrument with or without the handheld installed in the saddle.
To connect to a ProODO, simply align the saddle to the oval section on top of
the instrument and push it down to snap it in place (Figure 7).
Figure 7. Locate the oval alignment groove at the top of the instrument
and inside the saddle. Insert the saddle into this oval groove. Press the
saddle towards the back of the instrument until it snaps into place.
Connect the USB cable to the top of the saddle and to a USB port on the PC.
Once Data Manager is launched, the program will recognize all saddles with
instruments connected to the PC.
The instrument will be powered through the saddle and USB connection when
connected to the PC.
Manage Logged Data
Data that has been logged to the ProODO can be uploaded to the PC via the
provided USB saddle. You can upload sensor data, GLP files, site lists, and
instrument configuration files individually or all at once. After connecting the
instrument to the PC via the USB saddle and cable and launching Data Manager,
click the Retrieve Instrument Data tab. Click on the Instrument’s Unit ID you
would like to retrieve data from, then select the files you would like to retrieve
and click Start.
Once the sensor data is uploaded to the PC, you can graph and view tabular data
by instrument Unit ID, date/time, site name, and/or folder name. This allows
you to configure the report according to your needs. You can select to view
all data from all instruments, or select a certain date/time range for only a few
specific instruments, there are multiple ways to view the data. Once the report
has been defined, you will be able to print the graph and/or export the table.
28
Data Manager takes information management one step further and allows
you to delete specific points instead of entire files. This allows you to clean up
data that is no longer needed or that may have been collected erroneously, for
example, when the sensor was out of the water. If you can not delete data due
to regulation and compliance purposes, Data Manager has the solution. While
viewing logged data or real time data, you have the ability to ‘tag’ individual data
points with comments.
In addition to sensor data, you will be able to view GLP files, site lists, and
configuration files that have been uploaded from the instrument. These can be
printed and exported as well.
Real Time Data Collection
Data Manager allows you to view real time data on the PC.
After selecting your instrument, click the Real Time Instrument Data tab. Next,
input your sample interval, site/folder name, select the parameters you wish to
view and click OK. You must click Start on the next screen to begin your real
time study. Choose to hide the table or graph by unchecking the box next to
these options. Click Stop, then Edit Setup to change the Y-scale min/max of
the graph, to select different colors, or to name your graph. Add a comment
to a data point, by clicking in the comment field of the table next to the data
point. You may also Print the graph and Export the data for viewing in another
program.
Configure Instruments
Data Manager allows for easy and quick configuration of single or multiple
instruments. Once you have uploaded a site list or configuration file, you can edit
it as needed, save it, and download it to other instruments. You no longer need
to configure each instrument individually. By using the same configuration file
for all instruments, you can rest assured that all instruments will have identical
settings.
New site lists and configuration files can be created in Data Manager. These
lists and files can be downloaded to one or multiple instruments. Save time
by creating these files on your PC and downloading them to the instrument as
opposed to creating them on the instrument.
Minimum system requirements for Data Manager are a system with Windows®
2000 with SP4 (minimum) or Windows® XP with SP2 (minimum), 300 MHz or
higher Pentium®-compatible CPU, 128 MB of RAM or higher, 80 MB or more of
free hard-disk space, USB 2.0, and Microsoft® .NET.
29
M aintenance and S torage
This section describes the proper procedures for care, maintenance and storage
of the sensors. The goal is to maximize their lifetime and minimize down-time
associated with improper sensor usage.
General Maintenance - Battery
Compartment gasket
The instrument utilizes a gasket as a seal to prevent water from entering the
battery compartment. If the gasket and sealing surfaces are not maintained
properly, it is possible that water can enter the battery compartment. This
could severely damage the battery terminals causing loss of battery power and
corrosion to the battery terminals. Therefore, when the battery compartment
lid is removed, the gasket that provides the seal should be carefully inspected for
contamination (e.g. debris, grit, etc.) and cleaned if necessary. If contamination
is evident, clean the gasket and nearby plastic parts with lens cleaning tissue or
equivalent lint-free cloth. Alcohol can be used to clean the plastic parts, but use
only water and mild detergent on the o-ring itself. Also, inspect the gasket for
nicks and imperfections.
i
Using alcohol on a gasket may cause a loss of
elasticity and may promote cracking.
Sensor Maintenance - Dissolved Oxygen
Sensor Cap Replacement
The Sensor Cap should be replaced about once per year for highest data accuracy
It should also be replaced if it is cracked or damaged (see Troubleshooting
section for instruction on how to inspect the cap for damage). The instruction
sheet shipped with the replacement ODO Sensor Cap includes the calibration
coefficients specific to your Sensor Cap.
i
IMPORTANT - Be sure to save the ODO Sensor Cap instruction
sheet in case you need to reload the calibration coefficients.
The replacement ODO Sensor Cap is shipped in a humidified container and the
package should not be opened until immediately before sensor cap replacement.
Once the sensor cap has been installed on the ODO sensor as described below,
30
it is important to keep the sensor in a 100% humid environment. Therefore,
the ODO sensor should be stored either in the grey calibration/storage sleeve
with the sponge moistened or immersed in water, see Sensor Storage for more
information. If the sensor dries out, refer to Rehydration procedure in this
manual.
Refer to Figure 8 below when following the instructions for replacing the cap.
Figure 8
u
Caution: Avoid touching the sensing end of the sensor
cap during the following maintenance procedures.
1. Remove the old sensor cap assembly from the sensor by grasping the probe
body with one hand and then rotating the sensor cap counterclockwise until
it is completely free. Do not use any tools for this procedure.
2. Inspect the o-ring on the probe for damage. If there is any indication of
damage, carefully remove the o-ring and replace it with the new o-ring
included with the replacement Sensor Cap. Do not use any tools to remove
the o-ring.
3. Remove the new sensor cap from its hydrated container and dry the inside
cavity of the sensor cap with lens cleaning tissue. Make sure that the cavity
is completely dry before proceeding with the installation. Next, clean
the clear surface of the sensor on the end of the probe with lens cleaning
tissue.
4. Using clockwise motion, thread the new sensor cap onto the probe assembly
until it is finger-tight. The o-ring should be compressed between the sensor
31
cap and probe. Do not over-tighten the sensor cap and do not use any tools
for the installation process.
5. After installing the new sensor cap, store the sensor in either water or in
humidified air in the calibration sleeve.
6. Follow the procedures below for Configuring the ProODO instrument for
the new Sensor Cap.
Configuring ProODO Instrument for new Sensor Cap
After installing a new Sensor Cap, connect the probe/cable assembly to the
ProODO instrument and turn the instrument on. Locate the Calibration Code
Label at the top of the instruction sheet and note the six numbers which are
listed as K1 through K5 and KC. These six numbers contain the calibration
code for this particular sensor cap. Follow the instructions below to enter the
new calibration coefficients into your existing ODO instrument.
1. Press the Probe
, highlight DO, and press enter.
2. Highlight Sensor Cap and press enter.
3. Highlight Sensor Cap Coefficients and
press enter.
4. Highlight each coefficient in turn (K1
through KC) and use the numeric entry
screen to enter the corresponding new
coefficient from the Calibration Code
Label. Press Enter after each entry and
then proceed to the next K selection.
5. After all the new coefficients have been
entered, highlight Update Sensor Cap
Coefficients and press the Enter.
6. A message will appear warning that you
will be overwriting the current sensor cap
coefficients and you should confirm that
you wish to carry out this action. Highlight Yes and press enter to confirm
the new coefficients.
After updating the Coefficients, the Serial # in the Sensor Cap menu will be
updated automatically based on your entries. The Temperature Coefficient
32
listed in the Sensor Cap menu is programmed into the sensor at the factory and
should not be modified unless instructed by YSI Technical Support.
If errors are made in entering the Sensor Cap Coefficients, the instrument will
block the update and an error message will appear on the display. If you see
this error message, re-enter the coefficients and check them carefully for correct
transcription from the Calibration Code Label prior to selecting Update Sensor
Cap Coefficients. If you continue to get an error message after several entry
attempts, contact YSI Technical Support for assistance.
After entering the Sensor Cap Coefficients, perform a 1-point calibration.
Rehydrating the Sensor Cap
The Sensor Cap must remain in a moist environment; see Sensor Storage for
storage recommendations. If you inadvertently leave your sensor exposed to
ambient air for a period of more than approximately 8 hours it may dry out.
If the sensor membrane is allowed to dry out, it is likely to drift slightly at the
beginning of your next study unless it is rehydrated. If the cap dries out, you
can rehydrate it by soaking the probe tip with the sensor cap installed in warm
tap water for 24 hours. After rehydration is complete, recalibrate and be sure to
store the probe in a moist environment.
Sensor Maintenance - Temperature
You must keep the temperature portion of the sensor free of build up. Other
than that, the sensor requires no maintenance. A soft bristle brush, like a tooth
brush can be used to scrub the temperature sensor if needed.
Sensor Storage
Short-term Storage
When the ODO Sensor is not in use, it MUST BE STORED IN A MOIST
ENVIRONMENT, i.e., either in water or in water-saturated air. If the sensor
membrane is allowed to dry out by exposure to ambient air, it is likely to drift
slightly at the beginning of your next study unless it is rehydrated.
For short-term storage (<30 days), moisten the sponge in the calibration/storage
sleeve with a small amount of clean water and place this over the probe with the
sensor cap installed. This will provide a 100% saturated air environment.
33
Long-term Storage
For long-term storage (>30 days), remove the batteries from the instrument.
Moisten the sponge in the red protective plastic cap that was provided with the
probe and place it over the sensor with the sensor cap installed. Inspect the
sponge every 30 days to make sure it is still moist. If you no longer have the red
protective cap, then moisten the sponge in the calibration/transport sleeve and
place this over the probe.
Alternatively, you can place the probe with sensor cap directly in a beaker or
other container of water, making sure that the water does not evaporate over
time.
Long-term Storage: -5 to 70°C (23 to 158°F)
T roubleshooting
Dissolved Oxygen Readings
Erroneous dissolved oxygen readings typically indicate a need to service the
sensor cap, replace the sensor cap, and/or recalibrate. First, verify the sensor
is properly setup in the Probe menu, then follow the steps below to inspect the
sensor cap for damage and attempt to calibrate the sensor. If the problem persists,
try to rehydrate the sensor cap then recalibrate. If you are still getting erroneous
dissolved oxygen readings, try replacing the sensor cap and recalibrate. If the
erroneous readings persist, contact YSI Technical Support to help determine the
next step.
Inspecting the Sensor Cap for Damage
u
Caution: Avoid touching the sensing end of the sensor
cap during the following maintenance procedures.
If readings seem to be in error or are jumpy, remove the sensor cap from the
sensor by grasping the probe body with one hand and then rotating the sensor
cap counterclockwise until it is completely free. Avoid using tools for the
removal of the cap if at all possible.
Inspect the sensor cap assembly for any cracks or damage. If damage has
occurred, contact YSI Customer Service to order a replacement sensor cap.
Inspect the o-ring on the probe and the gasket at the top of the threads for
damage. If there is any indication of damage, carefully remove these parts and
34
contact YSI Technical Support to obtain a new o-ring and/or gasket. Avoid
using tools to remove the o-ring as damage to the sealing surfaces could result.
Before reinstalling the sensor cap, make sure that the cavity is completely dry
before proceeding with the installation. If water is found, dry the cavity with
lens cleaning tissue. Finally, clean the clear window on the end of the probe with
lens cleaning tissue.
After reinstalling a membrane cap, reevaluate the quality of the dissolved oxygen
readings. If problems persist, contact YSI Technical Support.
Help
During use of the ProODO instrument press Question
view help messages directly on the display.
from any screen to
Error/Status messages
If readings for a certain parameter are over range you will see a series of +++++
and if the readings are under range you will see a series of ----- plus the error
message along the bottom of the screen. If you see a series of ?????, that will
indicate that a certain parameter cannot be calculated. This could be due to a
connection issue between the cable and instrument. The following are some of
the potential messages:
Message
Description and Recommended Action
Probe Temp over range
Temperature is over 100C or reading
erroneously. Check cable connection and
ensure Temp sensor is clean.
Probe Temp under range
Temperature is under -10C or reading
erroneously. Check cable connection and
ensure Temp sensor is clean.
DO over range
DO% saturation is over 550%. Check sensor
cap and recalibrate.
DO under range
DO% saturation is under -5%. Check sensor
cap and recalibrate.
Barometer over range
Barometric pressure is reading over 988
mmHg. Calibrate barometer.
Barometer under range
Barometric pressure is reading under 375
mmHg. Calibrate barometer.
ODO Communications
Error
No communication between the instrument
and cable. Check cable connection.
35
Message
Description and Recommended Action
Clock Battery Low
Internal battery for real time clock has low
voltage. Contact Technical Support.
Measurements Locked!
Measurements are held in Manual Sampling
mode. Select Update Measurements or Log
Held Data.
Illegal Value may appear during alpha/numeric entry on the message line. This
only appears if the values entered do not match the formatting. This will also
appear in the GLP security area if the password is incorrect.
Restore Default Calibration Values
Occasionally, the instrument may need to have the factory calibration default
values restored. In order to accomplish this press Calibrate
, highlight
Restore Default Cal and press enter. Select the parameter you wish to restore,
either DO or Barometer, and press enter. After selecting barometer or DO, you
will be asked to confirm the operation. Highlight Yes and press enter.
A ccessories / P art N umbers
Part Number
Description
626281
ProODO Instrument
626250-1, -4, -10, -20, -30,
-40, -50, -60, or -100
1, 4, 10, 20, 30, 40, 50, 60, or
100-meter probe/cable assembly*
626230
Replacement Sensor Cap
36
Part Number
Description
605604
Communications saddle kit
605515
Data Manager desktop software
603075
Carrying case, soft-sided
603074
Carrying case, hard-sided
603069
Belt clip
37
Part Number
Description
063517
Ultra clamp
063507
Tripod clamp
603062
Cable management kit
605978
Weight, sensor/cable, 4.9 oz.
063019
Weight, sensor/cable, 24 oz., 3”
063020
Weight, sensor/cable, 51 oz., 6”
603070
Shoulder strap
*All cables include a temperature and dissolved oxygen sensor. Special order
cables in 10 meter increments from 60 to 100 meters are available.
38
D eclaration of C onformity
The undersigned hereby declares on behalf of the named manufacturer under our
sole responsibility that the listed product conforms to the requirements for the listed
European Council Directive(s) and carries the CE mark accordingly.
Manufacturer:
YSI Incorporated
1725 Brannum Lane
Yellow Springs, OH 45387
USA
Product Name:
Professional ODO Water Quality Instrument
Model Numbers
Instrument/Accessory:
ProODO (626281) / ProComm (605604)
Probe/Cable Assemblies:
626250
Conforms to the following:
Directives:
EMC 2004/108/EC
RoHS 2002/95/EC
WEEE 2002/96/EC
Harmonized Standards:
• EN61326-1:2006, Electrical equipment for
measurement, control, and laboratory use – EMC
requirements – Part 1: General Requirements
• EN61326-2-3:2006, Electrical equipment for
measurement, control and laboratory use – EMC
requirements – Part 2-3: Particular Requirements
– Test configuration, operational conditions,
and performance criteria for transducers with
integrated or remote signal conditioning.
• EN61000-3-2:2006, Electromagnetic
compatibility (EMC) – Part 3-2: Limits
– Limits for harmonic current emissions
(equipment input current < 16A per phase).
• EN61000-3-3:1995 +A1:2001 +A2:2005,
Electromagnetic compatibility (EMC) – Part
3: Limits – Section 3: Limitation of voltage
fluctuations and flicker in low-voltage supply
systems for equipment with rated current < 16A.
39
Supplementary
Information:
All performance met the continuous
unmonitored operation criteria as follows:
1. ESD, EN61000-4-2, Performance Criterion B
2. Radiated Immunity, EN61000-43, Performance Criterion A
3. EFT, EN61000-4-4, (EFT) Performance Criterion B
4. Surge, EN61000-4-5, Performance Criterion B
5. Conducted Immunity, EN610004-6, Performance Criterion A
6. Voltage Interrupts, EN61000-411, Performance Criterion B
7. RF Emissions, EN55011:1998,
A1:1999 Class B equipment
Authorized EU
Representative
YSI Hydrodata Ltd
Unit 8, Business Centre West, Avenue 1
Letchworth, Hertfordshire, SG6 2HB UK
Signed: Lisa M. Abel
Title: Director of Quality
Date: 26 November 2008
The undersigned hereby declares on behalf of the named manufacturer under our
sole responsibility that the listed product conforms to the requirements for electrical
equipment under US FCC Part 15 and ICES-003 for unintentional radiators.
Manufacturer:
YSI Incorporated
1725 Brannum Lane
Yellow Springs, OH 45387
USA
Product Name:
Professional ODO Water Quality Instrument
Model Numbers
Instrument/Accessory:
ProODO (626281) / ProComm (605604)
Probe/Cable Assemblies:
626250
Conforms to the following:
Standards:
• FCC 47 CFR Part 15-2008, Subpart B,
Class B, Radio Frequency Devices
• ICES-003:2004, Digital Apparatus
Supplementary
Information:
Tested using ANSI C63.4-2003 (excluding
sections 4.1, 5.2, 5.7, 9, and 14)
Signed: Lisa M. Abel
Title: Director of Quality
40
Date:: 26 November 2008
The undersigned hereby declares on behalf of the named manufacturer under
our sole responsibility that the listed product conforms with the Australian and
New Zealand Electromagnetic Compatibility (EMC) requirements for generic
products to be used in residential, commercial, and light industrial environments.
Manufacturer:
YSI Incorporated
1725 Brannum Lane
Yellow Springs, OH 45387
USA
Product Name:
Professional ODO Water Quality Instrument
Model Numbers
Instrument/Accessory:
ProODO (626281) / ProComm (605604)
Probe/Cable Assemblies:
626250
Conforms to the following:
Standards:
• AS/NZS 4251.1:1999, Electromagnetic
Compatibility (EMC) – Generic
emission standard – Part 1: Residential,
commercial, and light industry.
Signed: Lisa M. Abel
Date: 26 November 2008
Title: Director of Quality
R ecycling
YSI is committed to reducing the environmental footprint in the course of
doing business. Even though materials reduction is the ultimate goal, we know
there must be a concerted effort to responsibly deal with materials after they’ve
served a long, productive life-cycle. YSI’s recycling program ensures that old
equipment is processed in an environmentally friendly way, reducing the amount
of materials going to landfills.
•
•
•
Printed Circuit Boards are sent to facilities that process and reclaim as much
material for recycling as possible.
Plastics enter a material recycling process and are not incinerated or sent
to landfills.
Batteries are removed and sent to battery recyclers for dedicated metals.
When the time comes for you to recycle, follow the easy steps outlined at
www.ysi.com/recycle.
41
C ontact I nformation
Ordering and Technical Support
Telephone: 800 897 4151 (US)
+1 937 767 7241 (Globally)
Monday through Friday, 8:00 AM to 5:00 ET
Fax: +1 937 767 9353 (orders)
+1 937 767 1058 (technical support)
Email: environmental@ysi.com or proseries@ysi.com
Mail: YSI Incorporated
1725 Brannum Lane
Yellow Springs, OH 45387
USA
Internet: www.ysi.com
When placing an order please have the following available:
1.) YSI account number (if available)
2.) Name and phone number
3.) Purchase Order or Credit Card
4.) Model Number or brief description
5.) Billing and shipping addresses
6.) Quantity
Service Information
YSI has authorized service centers throughout the United States and
Internationally. For the nearest service center information, please visit www.
ysi.com and click ‘Support’ or contact YSI Technical Support directly at 800897-4151.
When returning a product for service, include the Product Return form with
cleaning certification. The form must be completely filled out for an YSI Service
Center to accept the instrument for service. The form may be downloaded from
www.ysi.com by clicking on the ‘Support” tab, then the Product Return Form
button.
42
A ppendix A - D O % C alibration
Values
C a l i b r at i o n Pressure
Value
D.O. %
in Hg
mmHg
kPa
mbar
101%
30.22
767.6
102.34
1023.38
100%
29.92
760.0
101.33
1013.25
99%
29.62
752.4
100.31
1003.12
98%
29.32
744.8
99.30
992.99
97%
29.02
737.2
98.29
982.85
96%
28.72
729.6
97.27
972.72
95%
28.43
722.0
96.26
962.59
94%
28.13
714.4
95.25
952.46
93%
27.83
706.8
94.23
942.32
92%
27.53
699.2
93.22
932.19
91%
27.23
691.6
92.21
922.06
90%
26.93
684.0
91.19
911.93
89%
26.63
676.4
90.18
901.79
88%
26.33
668.8
89.17
891.66
87%
26.03
661.2
88.15
881.53
86%
25.73
653.6
87.14
871.40
85%
25.43
646.0
86.13
861.26
84%
25.13
638.4
85.11
851.13
83%
24.83
630.8
84.10
841.00
82%
24.54
623.2
83.09
830.87
81%
24.24
615.6
82.07
820.73
80%
23.94
608.0
81.06
810.60
79%
23.64
600.4
80.05
800.47
78%
23.34
592.8
79.03
790.34
77%
23.04
585.2
78.02
780.20
76%
22.74
577.6
77.01
770.07
75%
22.44
570.0
75.99
759.94
74%
22.14
562.4
74.98
749.81
73%
21.84
554.8
73.97
739.67
72%
21.54
547.2
72.95
729.54
43
A ppendix B - O xygen S olubility
T able
Solubility of Oxygen in mg/L in Water Exposed to Water-Saturated Air at 760
mm Hg Pressure.
Salinity = Measure of quantity of dissolved salts in water.
Chlorinity = Measure of chloride content, by mass, of water.
S(0/00) = 1.80655 x Chlorinity (0/00)
Temp °C
Chlorinity : 0
Salinity: 0
5.0 ppt
9.0 ppt
10.0 ppt 15.0 ppt 20.0 ppt 25.0 ppt
18.1 ppt 27.1 ppt 36.1 ppt 45.2 ppt
0.0
14.62
13.73
12.89
12.10
11.36
10.66
1.0
14.22
13.36
12.55
11.78
11.07
10.39
2.0
13.83
13.00
12.22
11.48
10.79
10.14
3.0
13.46
12.66
11.91
11.20
10.53
9.90
4.0
13.11
12.34
11.61
10.92
10.27
9.66
5.0
12.77
12.02
11.32
10.66
10.03
9.44
6.0
12.45
11.73
11.05
10.40
9.80
9.23
7.0
12.14
11.44
10.78
10.16
9.58
9.02
8.0
11.84
11.17
10.53
9.93
9.36
8.83
9.0
11.56
10.91
10.29
9.71
9.16
8.64
10.0
11.29
10.66
10.06
9.49
8.96
8.45
11.0
11.03
10.42
9.84
9.29
8.77
8.28
12.0
10.78
10.18
9.62
9.09
8.59
8.11
13.0
10.54
9.96
9.42
8.90
8.41
7.95
14.0
10.31
9.75
9.22
8.72
8.24
7.79
15.0
10.08
9.54
9.03
8.54
8.08
7.64
16.0
9.87
9.34
8.84
8.37
7.92
7.50
17.0
9.67
9.15
8.67
8.21
7.77
7.36
18.0
9.47
8.97
8.50
8.05
7.62
7.22
19.0
9.28
8.79
8.33
7.90
7.48
7.09
20.0
9.09
8.62
8.17
7.75
7.35
6.96
21.0
8.92
8.46
8.02
7.61
7.21
6.84
22.0
8.74
8.30
7.87
7.47
7.09
6.72
23.0
8.58
8.14
7.73
7.34
6.96
6.61
24.0
8.42
7.99
7.59
7.21
6.84
6.50
25.0
8.26
7.85
7.46
7.08
6.72
6.39
44
Temp °C
Chlorinity : 0
Salinity: 0
5.0 ppt
9.0 ppt
10.0 ppt 15.0 ppt 20.0 ppt 25.0 ppt
18.1 ppt 27.1 ppt 36.1 ppt 45.2 ppt
26.0
8.11
7.71
7.33
6.96
6.62
6.28
27.0
7.97
7.58
7.20
6.85
6.51
6.18
28.0
7.83
7.44
7.08
6.73
6.40
6.09
29.0
7.69
7.32
6.93
6.62
6.30
5.99
30.0
7.56
7.19
6.85
6.51
6.20
5.90
31.0
7.43
7.07
6.73
6.41
6.10
5.81
32.0
7.31
6.96
6.62
6.31
6.01
5.72
33.0
7.18
6.84
6.52
6.21
5.91
5.63
34.0
7.07
6.73
6.42
6.11
5.82
5.55
35.0
6.95
6.62
6.31
6.02
5.73
5.46
36.0
6.84
6.52
6.22
5.93
5.65
5.38
37.0
6.73
6.42
6.12
5.84
5.56
5.31
38.0
6.62
6.32
6.03
5.75
5.48
5.23
39.0
6.52
6.22
5.98
5.66
5.40
5.15
40.0
6.41
6.12
5.84
5.58
5.32
5.08
41.0
6.31
6.03
5.75
5.49
5.24
5.01
42.0
6.21
5.93
5.67
5.41
5.17
4.93
43.0
6.12
5.84
5.58
5.33
5.09
4.86
44.0
6.02
5.75
5.50
5.25
5.02
4.79
45.0
5.93
5.67
5.41
5.17
4.94
4.72
45
Item # 626279
Rev B
Drawing # A626279
December 2008
©2008 YSI Incorporated.
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