Eppendorf Top Buret Operating Manual

Below you will find brief information for Top Buret M/H. The Top Buret M/H is a bottle-top burette designed for precise and pulse-free dispensing of solutions. It supports titration up to 1 mol/L concentration limits. The device offers features such as easy venting, calibration, and adjustment. It includes accessories like bottle thread adapters. It is essential for accurate measurements and minimizing errors in the dispensing process. The device allows for a safe and efficient titration process.

PDF

Document

Top Buret M/H

Operating Manual

Eppendorf ® and the Eppendorf Brand Design are registered trademarks of Eppendorf SE,

Germany.

Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.

U.S. Design Patents are listed on www.eppendorf.com/ip

4965 900.018-07/122021



Table of contents

Top Buret M/H

English (EN)

1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.1

Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2

Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.1

Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.3

1.4

1.2.2

Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.1

Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.2

2.3

Symbols on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Application limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Physical properties of the liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Unsuitable liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.3.3

Suitable liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3.1

Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3.2

3.1.1

Accessories – bottle thread adapter . . . . . . . . . . . . . . . . . . . . . . . 13

Product overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Telescopic aspirating tube. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3.2.3

Thread adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.1

Mounting the dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.2

4.3

4.4

Mounting the canula arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Inserting the batteries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Mounting the telescopic aspirating tube . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.4.1

Adjusting the telescopic aspiration tube to the height of

 the bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Shortening the telescopic aspiration tube for smaller bottles. . . . 23

Inserting the telescopic aspirating tube . . . . . . . . . . . . . . . . . . . . 23

Screwing the dispenser onto the bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.5.1

Determining the diameter of the flask neck . . . . . . . . . . . . . . . . . 24

4.5.2

Screwing on the thread adapter . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Connecting the drying tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Table of contents

Top Buret M/H

English (EN)

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.1

Venting the dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.2

5.3

Titrating liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Changing the supply bottle during titration . . . . . . . . . . . . . . . . . . . . . . . . . 29

5.4

5.5

Removing the bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Emptying the dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

6 Calibration and adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

6.1

Calibrating the dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

6.2

Adjusting the dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

6.2.1

Adjustment to distilled water. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

6.3

6.2.2

Adjustment to a liquid with a different density . . . . . . . . . . . . . . . 32

Resetting the Top Buret to the factory settings . . . . . . . . . . . . . . . . . . . . . . 33

7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

7.1

Error search. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

8 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.1

Cleaning the dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.1.1

8.1.2

Standard cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Intensive cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

8.2

8.3

Disassembling the dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Replacing the batteries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

9.1

Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

9.2

9.3

9.4

Errors of measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

Parameters for titratable liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

Conditions for calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

10.1

Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

10.2

Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

10.3

Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

11 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

11.1

Dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

11.2

Spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

11.3

Thread adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Operating instructions

Top Buret M/H

English (EN)

1

1.1 Operating instructions

Using this manual



Read this operating manual completely before using the device for the first time.

Observe the instructions for use of the accessories where applicable.



This operating manual is part of the product. Please keep it in a place that is easily accessible.



Enclose this operating manual when transferring the device to third parties.



The current version of the operating manual for all available languages can be found on our webpage www.eppendorf.com/manuals.

1.2 Danger symbols and danger levels

1.2.1 Danger symbols

The safety instructions in this manual have the following danger symbols and danger levels:

Biohazard Explosive substances

Hazard point Toxic substances

Material damage

1.2.2 Danger levels

DANGER

WARNING

CAUTION

NOTICE

Will lead to severe injuries or death.

May lead to severe injuries or death.

May lead to light to moderate injuries.

May lead to material damage.

1.3 Symbols used

Depiction Meaning



Actions in the specified order

Actions without a specified order

• List

Text Display or software texts

Additional information

Operating instructions

Top Buret M/H

English (EN)

1.4 Abbreviations used

ETFE

Ethylene-tetrafluoroethylene copolymer

FEP

Tetrafluorethylene-hexafluorpropylene Copolymer

FKM

Fluororubber

PFA

Perfluoroalkoxy copolymer

Polypropylene

PTFE

Polytetrafluorethylene

PVDF

Polyvinylidene fluoride

Safety

Top Buret M/H

English (EN)

2

2.1 Safety

Intended use

The Top Buret is a product generally used in the laboratory for dispensing aqueous solutions. Applications in or on the human body (in vivo applications) are not permitted.

The dispenser may only be used within the specified technical and physical limits.

The dispenser must not be exposed to an aggressive atmosphere (e.g., HCI vapors). The dispenser must not be autoclaved.

2.2

Symbol

Symbols on the device

Meaning

General warning sign

Read instructions

Wear protective eyewear

Wear hand protection

Wear protective clothing

Safety

Top Buret M/H

English (EN)

Symbol Meaning

Device meets the European requirements for safety, health protection and environmental protection.

Device meets the requirements for safety, health protection and environmental protection in Great Britain.

Device meets the requirements for the authorized use of harmful substances in the People's Republic of China.

Dispose of the device properly.

Symbol for the proper disposal of electronic equipment on

European markets.

Safety

Top Buret M/H

English (EN)

2.3 Application limits

2.3.1 Physical properties of the liquid

Density (maximum) 2,2 g/cm

Concentration (maximum) 1 mol/L

Vapor pressure (maximum) 50 kPa

2.3.2 Unsuitable liquids

NOTICE! Material damage due to incorrect handling

Deposits that are hard to dissolve lead to irreparable damage on the piston, the valves, and the discharge tube.



Only use approved liquids.

The dispenser is not suitable for the following solutions, substances, and liquids:

• Liquids with low ignition temperatures (e.g., carbon disulfide).

• Liquids which attack ETFE, FEP, PFA, PTFE or Boro 3.3.

• Solutions containing hydrofluoric acid.

• Suspensions, as solid particles can clog or damage the device (e.g. activated carbon).

• Liquids which form insoluble deposits and decomposing solutions (e.g. Biuret reagent).

• Fuming acids or highly concentrated alkaline solutions

• Substances which react catalytically with platinum iridium (e.g. H

• Solutions prone to crystallization.

Safety

Top Buret M/H

English (EN)

2.3.3 Suitable liquids

The dispenser is suitable for the following titration liquids up to a concentration of



1 mol/L:

• Ammonium iron(II) sulfate solution

• Ammonium thiocyanate solution

• Barium chloride solution

• Bromid bromate solution

• Cerium(IV) sulfate solution

• EDTA solution

• Iron(II) sulfate solution

• Acetic acid

• Caustic potash solution

• Potassium bromate solution

• Potassium bromide bromate solution

• Potassium dichromate solution

• Potassium iodate solution

• Potassium permanganate solution

• Potassium thiocyanate solution

• Sodium arsenite solution

• Sodium carbonate solution

• Sodium chloride solution

• Sodium nitrite solution

• Sodium thiosulfate solution

• Caustic soda

• Oxalic acid

• Perchloric acid

• Nitric acid

• Hydrochloric acid

• Sulfuric acid

• Silver nitrate solution

• Tetra-n-butylammonium hydroxide solution

• Zinc sulfate solution

Refer to the information provided by the reagent manufacturers. If you have any questions, contact our authorized Eppendorf Service.

Safety

Top Buret M/H

English (EN)

2.4 User profile

The device and accessories may only be operated by trained and skilled personnel.

Before using the device, read the operating manual and the instructions for use of the accessories carefully and familiarize yourself with the device's mode of operation.

2.5 Warnings for intended use

DANGER! Risk of explosion.



Do not use the device in an explosive atmosphere.



Do not operate the device in areas where work with explosive substances is carried out.



Do not use this device to process any explosive or highly reactive substances.



Do not use this device to process any substances which could create an explosive atmosphere.

WARNING! Damage to health due to infectious liquids and pathogenic germs.



When handling infectious liquids and pathogenic germs, observe the national regulations, the biosafety level of your laboratory, and the manufacturers'

Safety Data Sheets and application notes.



Wear your personal protective equipment.



Consult the "Laboratory Biosafety Manual" (source: World Health

Organization, Laboratory Biosafety Manual, as amended) for comprehensive regulations on the handling of germs or biological material of risk group II or higher.

WARNING! Damage to health due to toxic, radioactive or aggressive chemicals.



Wear your personal protective equipment.



Observe the national regulations for handling these substances.



Observe the manufacturers' Safety Data Sheets and application notes.

Safety

Top Buret M/H

English (EN)

CAUTION! Contamination due to contact with biological and chemical reagents.

Contact with reagents may be harmful to eyes or skin.



Wear your personal protective equipment.



Make sure that no reagents are leaking from the device.



Ensure that the piston can move freely before starting work.



Do not point the opening of the discharge tube towards people.



Only initiate liquid dispensing if you are not endangering other people.



Dispense liquid slowly and evenly to prevent splashing. Do not use force.



Only disassemble the device when it is clean.

CAUTION! Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by

Eppendorf may impair the safety, functioning and precision of the device.

Eppendorf cannot be held liable or accept any liability for damage resulting from the use of accessories and spare parts other than those recommended or from improper use.



Only use accessories and original spare parts recommended by Eppendorf.

CAUTION! Personal injury due to incorrect transportation of the device.

If the assembled device is not transported correctly, reagents will be released.

Contact with reagents may be harmful to eyes or skin.



To transport the assembled device, hold the head gear of the device with one hand and support the bottom of the bottle with the other hand.



Do not hold the device by the cylinder sleeve.

NOTICE! Risk of damage to device due to autoclaving.



Do not autoclave the device.

3

3.1

Quantity

Product description

Delivery package

Description

Top Buret

Canula arm

Discharge tube with knurled nut

Tube holder

Telescopic aspirating tube

Battery LR03/AAA, 1.5 V

Operating manual

3.1.1 Accessories – bottle thread adapter

Quantity

Description

GL 45/S 40

GL 45/GL 38

GL 45/GL 32

Product description

Top Buret M/H

English (EN)

Product description

Top Buret M/H

English (EN)

3.2

Abb. 3-1:Top Buret M/H

Product overview

Fig. 3-1: Top Buret M/H

1 Control panel

2 Dispensing wheels

3 Bottle thread

4 Telescopic aspirating tube

5 Discharge valve toggle

6 Canula arm

7 Discharge tube

8 Tube holder

3.2.1 Control panel

Abb. 3-2:Control panel with display and keys

Fig. 3-2: Control panel with display and keys

1 Pause symbol

2 Titration mode symbol

3 Battery empty symbol

4 Pause key

5 Start key

6 Calibration mode

7 Volume display

8 Arrow keys

9 Opening symbol

Product description

Top Buret M/H

English (EN)

Product description

Top Buret M/H

English (EN)

3.2.2 Telescopic aspirating tube

Abb. 3-3:Telescopic aspirating tube

Fig. 3-3: Telescopic aspirating tube

1 Connection opening

Inner tube

2 Seal

3.2.3 Thread adapter

Abb. 3-4:Thread adapter

3 Aspirating opening

Outer tube

Fig. 3-4: Thread adapter

1 Reducing from 45 mm to 40 mm

2 Reducing from 45 mm to 38 mm

2 3

3 Reducing from 45 mm to 32 mm

Product description

Top Buret M/H

English (EN)

3.3 Features

The Top Buret is a bottle-top buret which features a continuous, pulse-free dispensing technique. The Top Buret can dispense liquids with a maximum concentration of 1 mol/L.

When the dispensing wheels are fully rotated, the following volumes are dispensed:

• Top Buret M: 2.5 mL

• Top Buret H: 5.0 mL

The Top Buret has two dispensing settings. The dispensing settings are set with the toggle on the canula arm.

• Titration

Titrate liquid from the bottle to a destination vessel.

• Deairing

Rinse liquid through the discharge valve into the bottle, e.g., to remove air bubbles.

3.4 Materials

NOTICE! Aggressive substances may damage components, consumables and accessories.



Check the chemical resistance before using organic solvents or aggressive chemicals.



Only use liquids whose vapors do not attack the materials used.

CAUTION! Material damage caused by aggressive vapors.

The electronic components of the dispenser can be corroded by aggressive chemical vapors.



Do not expose the dispenser to aggressive vapors over an extended period of time.

Product description

Top Buret M/H

English (EN)

The assembly parts of the dispenser consist of the following materials:

Component Material

Suction pipe Ethylene-tetrafluoroethylene (ETFE), polytetrafluorethylene (PTFE), fluorinated ethylene propylene (FEP)

Discharge tube Tetrafluoroethylene-perfluoropropylen copolymer

(FEP)

Discharge valve, recirculation valve Perfluoroalkoxy alkane (PFA), polytetrafluorethylene (PTFE)

Valve block Borosilicate glass, PFA, platinum iridium (Pt-Ir)

Valve balls

Piston/Cylinder

Adapter

Borosilicate glass

PFA/borosilicate glass

Polypropylene (PP), ETFE, PTFE

4

4.1

Abb. 4-1:Overview of the individual parts

Installation

Mounting the dispenser

9 11

10 12

Fig. 4-1: Overview of the individual parts

1 Control panel

2 Dispensing wheels

3 Air vent cover

4 Valve head

5 Bottle thread

6 Connection opening

7 Telescopic aspirating tube

8 Recirculation tube

9 Discharge valve toggle

10 Discharge valve

11 Knurled nut

12 Canula arm

13 Discharge tube

14 Tube holder

15 Gripping nubs (inside)

Installation

Top Buret M/H

English (EN)

Installation

Top Buret M/H

English (EN)

4.2 Mounting the canula arm

1. Rotate the toggle to the deairing position.

2. Remove the toggle upwards.

3. Insert the tube holder into the canula arm with the gripping nubs pointing downwards.

4. Guide the discharge tube through the hole in the canula arm from below.

5. Insert the discharge tube through the tube holder from above.

The discharge tube should protrude slightly from the tube holder.

6. Screw the knurled nut onto the discharge valve.

Installation

Top Buret M/H

English (EN)

7. Slide the canula arm over the discharge valve from above up to the stop.

8. Attach the toggle.

4.3 Inserting the batteries

NOTICE! Damage to the battery compartment due to incorrect insertion of the batteries



Observe the correct polarity of the batteries.



Do not use force.

The display automatically switches on when the batteries are inserted



(1.5 V, LR03/AAA). If the device is not used, the display will switch off after about 5 minutes.

Installation

Top Buret M/H

English (EN)

1. Press and hold the opening symbol.

2. Slide the control panel upwards and remove it.

3. Remove the battery compartment lid at the back of the control panel.

4. Insert the batteries.

5. Insert the battery compartment lid.

6. Attach the control panel and slide it downwards until it engages.

4.4 Mounting the telescopic aspirating tube

The dispenser conveys the liquid through the telescopic aspirating tube from the supply bottle. Adjust the length of the telescopic aspirating tube to the bottle height to empty the bottle completely.

4.4.1 Adjusting the telescopic aspiration tube to the height of the bottle

Prerequisites

• The bottle is larger than the collapsed telescopic aspirating tube.

Use an empty bottle to adjust the telescopic aspiration tube.

1. Hold the telescopic aspiration tube next to the bottle.

2. Extend the telescopic aspiration tube.

The telescopic aspiration tube is supposed to reach from the bottle opening to just above the base of the bottle.

3. Cut the lower end of the outer tube off at an angle.

Installation

Top Buret M/H

English (EN)

4.4.2 Shortening the telescopic aspiration tube for smaller bottles

Prerequisites

• The bottle is smaller than the collapsed telescopic aspirating tube.

1. Shorten the inner tube.

2. Shorten the outer tube.

3. Cut the lower end of the outer tube off at an angle.

4.4.3 Inserting the telescopic aspirating tube

Prerequisites

• The telescopic aspiration tube is adjusted to the height of the bottle.

1. Cut the lower end of the outer tube off at an angle.

2. Slide the inner tube onto the connection opening up to the stop.

4.5 Screwing the dispenser onto the bottle

CAUTION! Personal injury due to incorrect transportation of the burette.

If the mounted burette is not transported correctly, reagents will be released.

Contact with reagents may be harmful to eyes or skin.



To transport the mounted burette, grasp the burette with one hand and the bottle with the other hand.



Do not carry the burette by the housing.

CAUTION! Personal injury due to contact with reagents.

Contact with reagents may be harmful to eyes or skin.



Wear your personal protective equipment.



Make sure that no reagents are leaking from the device.

Installation

Top Buret M/H

English (EN)

The thread connection of the dispenser is designed for bottle threads of 45 mm. For other bottle threads a thread adapter can be used.

Prerequisites

• The telescopic aspirating tube is inserted.

• The length of the telescopic aspirating tube has been adjusted to the height of the bottle.

1. Rotate the discharge valve toggle to deairing.

2. Attach the dispenser onto the bottle from above.

3. Screw the dispenser onto the bottle.

4.5.1 Determining the diameter of the flask neck

Prerequisites

• A suitable thread adapter is available.

Some thread adapters are included in the delivery package. Other diameters can be ordered. The diameter of the thread adapter is imprinted on the adapter. If the bottle thread is smaller than 32 mm, a second adapter must be used.

1. Measure the inner diameter of the bottle top or the outer diameter of the flask neck.

2. Choose the suitable thread adapter.

4.5.2 Screwing on the thread adapter

Prerequisites

• A suitable thread adapter is available.

1. Screw the thread adapter onto the flask neck.

2. Rotate the discharge valve toggle to deairing.

3. Attach the dispenser onto the bottle from above.

4. Screw the dispenser onto the bottle.

Installation

Top Buret M/H

English (EN)

4.6 Connecting the drying tube

For the titration of hygroscopic liquids you need a drying tube with a suitable liquid absorber.

The drying tube is not included in the delivery package and can be ordered as an accessory (without filling/liquid absorber).

Suitable liquid absorbers:

• Silica gel with a particle size of 1 mm – 3 mm

• CaCO

• NaOH pills with a diameter of 5 mm for the absorption of CO

1. Remove the air vent cover.

2. Insert the drying tube into the opening.

3. Fill the drying tube with moisture absorber.

Operation

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5

5.1 Operation

Rinse the dispenser thoroughly before first use or discard the first units dispensed.

Venting the dispenser

CAUTION! Personal injury due to contact with reagents.

Contact with reagents may be harmful to eyes or skin.



Wear your personal protective equipment.



Do not point the discharge tube at people.



Do not remove the discharge tube from the tube holder in order to avoid any splashing of reagents.

Air bubbles in the supply lines and in the feeding mechanics will distort the measuring results.

Deair the dispenser in the following situations:

• Prior to initial operation

• After intensive cleaning

• After changing the bottle

• After a long period of inactivity

Prerequisites

• The dispenser has been completely mounted.

1. Rotate the discharge valve toggle to deairing.

Operation

Top Buret M/H

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2. Remove the control panel.

3. Rotate the dispensing wheels slowly and evenly forward until there is no more air left in the system or in the dispensing hose.

4. Rotate the discharge valve toggle to titration.

5. Place a collection vessel below the canula.

6. Rotate the dispensing wheels forward until liquid emerges from the opening of the discharge tube.

7. Attach the control panel.

8. Wipe any chemical residues from the discharge tube.

Operation

Top Buret M/H

English (EN)

5.2 Titrating liquid

If you rotate the dispensing wheels backwards, they do not engage. No dispensing of liquid is performed and no volume is counted.

When you have completed or interrupted the titration, move the toggle to return dosing to prevent any liquid from inadvertently escaping.

If the dispenser is not used, the display will switch off after 5 minutes. The titrated volume is stored until the next titration.

1. Press the Start key.

The display switches on.

The display shows 0.00

or the volume of the last titration.

2. Press the Start key.

The volume display is set to 0.00

3. Pull out the canula arm to the desired length.

4. Hold a collection vessel below the discharge tube.

5. Rotate the toggle to titration.

6. Rotate the dispensing wheels forward evenly.

The liquid is dispensed.

7. After titration, rotate the toggle to deairing.

Operation

Top Buret M/H

English (EN)

5.3 Changing the supply bottle during titration

You can change the supply bottle during a titration sequence without changing the displayed volume.

Prerequisites

• The same chemical is used.

1. Press the Pause key.

On the display, the Stop symbol appears.

The titrated value is stored.

2. Remove the bottle.

3. Mount the new bottle.

4. Vent the dispenser.

5. Press the Pause key again.

The display is enabled. The display shows the stored value and the titration symbol .

6. Continue titration.

5.4 Removing the bottle

1. Rotate the discharge valve toggle to deairing.

2. Place the dispenser with bottle into a suitable collecting vessel.

3. Unscrew the dispenser from the bottle in a counterclockwise direction.

4. Lift the dispenser until the telescopic aspirating tube is no longer immersed in the liquid.

5. Gently tap the telescopic aspirating tube against the inside of the bottle.

The residual liquid from the telescopic aspirating tube runs into the bottle.

Operation

Top Buret M/H

English (EN)

5.5 Emptying the dispenser

Before changing the liquid and before cleaning, the dispenser must be emptied.

Prerequisites

• The bottle has been unscrewed.

1. Screw the dispenser onto an empty bottle.

2. Place a collection vessel below the canula.

3. Rotate the toggle to titration.

4. Rotate the dispensing wheels forward until no more liquid runs out of the discharge tube.

5. Rotate the toggle to deairing.

6. Rotate the dispensing wheels forward until no more liquid runs out of the recirculation tube.

To remove the reagents completely, clean the dispenser.

Calibration and adjustment

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6

6.1 Calibration and adjustment

Calibrating the dispenser

The gravimetric test and the conversion of the measured values for the volume are described in the document "Standard operating procedure for manual dispensing systems

(SOP)" . The document is available on the webpage www.eppendorf.com/manuals.

6.2 Adjusting the dispenser

The dispenser has been adjusted to the physical properties of demineralized water at the factory.

The dispenser can be readjusted:

• If the physical properties of the liquid are different to those of water.

• If the error of measurement of the dispensed volume is outside of the tolerance range.

Calibration is only possible with a testing volume between 10 mL – 90 mL.

If the testing volume is < 10 mL, the display shows:

If the testing volume is > 90 mL, the display shows:

To display the set adjustment value, either press the or arrow key in titration mode. After you release the key, the display automatically returns to the last volume display.

6.2.1 Adjustment to distilled water

Prerequisites

• The document "Standard operating procedure for manual dispensing systems – (SOP)" is available.

1. Measure the testing volume according to the method described in the SOP .

2. Use the average value of the volumes from the calibration.

3. Press the Pause key.

The volume is saved.

4. Press and hold the and arrow keys for 3 seconds.

The display shows CAL .

5. Set the calculated average value of the volumes using the and arrow keys.

Calibration and adjustment

Top Buret M/H

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6. Press the Start key.

• The set value is accepted.

• The display is reset to zero.

• The adjustment is ended.

• The display shows C at the top right. The display indicates that the factory settings have been changed.

6.2.2 Adjustment to a liquid with a different density

• The document "Standard operating procedure for manual dispensing systems – (SOP)" is available.

1. Set up the calibrated analytical balance at a vibration and draft-free location.

2. Place a beaker on the balance.

3. With the Top Buret M, titrate 25 mL of the test liquid into the beaker and record the weight.

4. Repeat step 3 10 times.

5. With the Top Buret H, titrate 50 mL of the test liquid into the beaker and record the weight.

6. Repeat step 5 10 times.

7. Calculate the volume of the standard solution according to the SOP .

8. Calculate the average value of the volumes according to the SOP .

9. Press the Pause key.

The volume is saved.

10.Press and hold the and arrow keys for 3 seconds.

The display shows CAL at the top right.

11.Set the calculated average value of the volumes using the and arrow keys.

12.Press the Start key.

• The set value is accepted.

• The display is reset to zero.

• The adjustment is ended.

• The display shows C . The display indicates that the factory settings have been changed.

Calibration and adjustment

Top Buret M/H

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6.3 Resetting the Top Buret to the factory settings

The reset function is used to restore the factory settings. If you have adjusted the device, the display will show a C . When you restore the factory settings again, the C will disappear.

1. Press the Start key to switch on the Top Buret.

2. Press and hold the Start and keys at the same time for 3 seconds to activate the reset function.

• The display C disappears.

• The factory settings have been restored.

Troubleshooting

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7

7.1 Troubleshooting

Error search

Problem

• Air is aspirated.

• There are air bubbles in the titrated liquid.

Cause

• The dispenser has not been sufficiently deaired.

Solution



Deair the dispenser



(see p. 26).

• The telescopic aspirating tube is not fully mounted.

No liquid is aspirated.

The titrating volume is too small.

• The telescopic aspirating tube is damaged.

• The telescopic aspirating tube does not immerse into the liquid.

• The connection opening is damaged.

• The connection opening is blocked.

• The telescopic aspirating tube is not fully mounted.

• The telescopic aspirating tube is damaged.

• The dispenser is adjusted incorrectly.

• The connection opening is damaged.

• The batteries are empty.

The icon appears on the display.

The dosing wheel is difficult to rotate.

• The piston is stiff.



Push the telescopic aspirating tube firmly onto the connection opening.



Shorten the telescopic aspirating tube or replace it.



Make the telescopic aspirating tube longer until it immerses into the liquid.



Send the dispenser for repair.



Clean the dispenser.



Push the telescopic aspirating tube firmly onto the connection opening.



Shorten the telescopic aspirating tube or replace it.



Set the dispenser to factory adjustment.



Send the dispenser for repair.



Replace the batteries.

Stop the dispensing procedure and clean the device.

Maintenance

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8

8.1 Maintenance

Cleaning the dispenser

CAUTION! Personal injury due to contact with reagents.

The feeding mechanism, valves, telescopic aspirating tube and discharge tube are filled with reagents. Reagents enter the bottle with the cleaning solution.

Contact with reagents may be harmful to eyes or skin.



Wear your personal protective equipment.



Discard the cleaning solution after cleaning.

The feeding mechanics is inside the device and consists of hoses and valves.

Hoses and valves can get heavily contaminated.

The feeding mechanics must only be cleaned by authorized skilled personnel. If the feeding mechanics need to be cleaned, please contact the authorized service.

Replace the discharge tube if it is heavily contaminated or damaged.

Clean the dispenser with a suitable disinfectant, e.g., 70 % ethanol.

Clean the dispenser in the following situations:

• Before a change of reagent.

• Prior to storage.

• If it has not been used for some time.

• Before maintenance and repairs.

• Daily, if concentrated alkaline solutions were used.

• Daily, if solutions which are prone to crystallization were used.

• Weekly, if the dispenser has been mounted to the storage bottle for some time.

• The dispensing wheels are hard to rotate.

• The dispenser is heavily contaminated.

Maintenance

Top Buret M/H

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8.1.1 Standard cleaning

Prerequisites

• The dispenser is empty.

• A neutral cleaning solution is available.

• Distilled water is available.

1. Mount the dispenser onto a bottle filled with a neutral cleaning solution.

2. Place a collection vessel below the discharge tube.

3. Switch the toggle to titration.

4. Rotate the dispensing wheels at least 10 times slowly and evenly forward.

5. Switch the toggle to deairing.

6. Rotate the dispensing wheels at least 10 times slowly and evenly forward.

7. Remove the dispenser from the bottle.

8. Rotate the dispensing wheels until the dispenser is completely emptied.

9. Screw the dispenser onto a bottle filled with distilled water.

10.Repeat steps 2 to 6.

11.Remove the dispenser from the bottle.

12.Rotate the dispensing wheels until the dispenser is completely emptied.

8.1.2 Intensive cleaning

Perform intensive cleaning in the following situations:

• The dispenser is heavily contaminated.

• The dispensing wheels are hard to rotate.

• You have titrated a highly crystalline liquid.

Prerequisites

• The dispenser has been cleaned according to the standard procedure.

• The dispenser has been disassembled.

1. Clean the telescopic aspirating tube with a soft bottle brush.

2. Clean all parts with a soft brush and a mild cleaning agent.

3. Wipe the housing without the control panel with a damp cloth and a mild cleaning agent.

4. Rinse all parts with distilled water.

5. Allow all assembly parts to dry.

6. Reassemble the dispenser.

Carry out assembly in the reverse order to disassembly.

7. Using distilled water, check that the dispenser is not leaking and that it is functioning correctly.

8. Adjust the dispenser.

Maintenance

Top Buret M/H

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8.2 Disassembling the dispenser

When disassembling the canula arm, be careful not to bend the discharge tube.

Prerequisites

• The dispenser has been cleaned according to the standard procedure.

1. Unscrew the dispenser from the bottle in a counterclockwise direction.

2. Remove the telescopic aspirating tube from the connection opening.

3. Remove the recirculation tube from the front hole in the valve head.

4. Rotate the discharge valve toggle to deairing and remove it upwards.

5. Pull off the canula arm in an upward motion.

6. Unscrew the discharge tube knurled nut from the discharge valve thread.

7. Remove the discharge tube from the discharge valve.

8. Remove the discharge tube from the canula holder and canula arm.

9. Remove the canula holder from the canula arm.

10.Pull the discharge valve out of the valve head.

Maintenance

Top Buret M/H

English (EN)

8.3 Replacing the batteries

Prerequisites

• New batteries are available.

If the battery capacity is exhausted, the icon will appear on the display. The batteries must be replaced.

1. Press and hold the opening symbol.

2. Slide the control panel upwards and remove it.

3. Remove the battery compartment lid at the back of the control panel.

4. Replace the batteries.

5. Replace the battery compartment lid.

6. Attach the control panel and slide it downwards until it engages.

7. Dispose of the old batteries in an environmentally friendly manner.

Technical data

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9

9.1 Technical data

Ambient conditions

Environment

Ambient temperature

Relative humidity

Atmospheric pressure

For indoor use only.

15 °C – 40 °C

0 % – 90 %, non-condensing.

Use up to a height of 2000 m above sea level.

9.2 Errors of measurement

Model M Testing volume

0.01 mL – 999.9 mL 2.5 mL

12.5 mL

25 mL

Systematic error

± % ± mL

2.0

0.4

0.05

0.2

0.05

Error

± %

Random error

± mL

1.0

0.2

0.025

0.1

0.025

Model H

0.01 mL – 999.9 mL

Testing volume

5 mL

25 mL

50 mL

Systematic error

± % ± mL

Error

± %

Random error

± mL

2.0

0.4

0.2

0.1

1.0

0.2

0.1

0.05

9.3 Parameters for titratable liquids

Medium temperature

Maximum density

15 °C – 40 °C

2.2 g/cm

Maximum vapor pressure 50 kPa

9.4 Conditions for calibration

Test conditions and test evaluation in accordance with ISO 8655, part 6. Test with analytical balance with evaporation protection, approved by the board of weights and measures.

The errors were determined under the following conditions:

Liquid

Number of determinations

Ambient temperature

Liquid dispensing

Water according to ISO 3696

20 °C – 25 °C; ± 0.5 °C

Dispensing on the tube inner wall

Transport, storage and disposal

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10.1 Transport, storage and disposal

Transport

Decontamination before shipment

If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer for disposal please note the following:

WARNING! Risk to health from contaminated device.

1. Observe the information contained in the decontamination certificate. It is available as a PDF document on our webpage



(https://www.eppendorf.com/decontamination).

2. Decontaminate all parts to be shipped.

3. Include the fully completed decontamination certificate in the shipment.

General transport

Air freight

NOTICE! Damage due to incorrect packing.

Eppendorf SE is not liable for any damage caused by improper packing.



Only store and transport the device in its original packing.

Air temperature

15 °C – 40 °C

-20 °C – 50 °C

Relative humidity Atmospheric pressure

0 % – 90 %, non-condensing.

Up to a height of 2000 m above sea level.

Up to a height of 12200 m above sea level.

10.2

Storage

Air temperature

15 °C – 40 °C

Relative humidity Atmospheric pressure

0 % – 90 %, non-condensing.

Up to a height of 2000 m above sea level.

Transport, storage and disposal

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10.3 Disposal

In case the product is to be disposed of, the relevant legal regulations are to be observed.

Information on the disposal of electrical and electronic devices in the European

Community:

Within the European Community, the disposal of electrical devices is regulated by national regulations based on EU Directive 2012/19/EU pertaining to waste electrical and electronic equipment (WEEE).

According to these regulations, any devices supplied after August 13, 2005, in the business-to-business sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. They are marked with the following symbol to indicate this:

As disposal regulations may differ from country to country within the

EU, please contact your supplier if necessary.

WARNING! Risk of explosion or fire due to overheated rechargeable batteries and batteries.



Do not heat rechargeable batteries and other batteries to temperatures above

60 °C and do not throw them into a fire.

Disposing of accumulators and batteries

Do not dispose of accumulators and batteries as household waste.

Dispose of accumulators and batteries according to the locally applicable legal regulations.

Ordering Information

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11

11.1 Ordering Information

Dispenser

Order no.

(International)

Description

4965 000.017

4965 000.025

Eppendorf Top Buret M

25 mL

Eppendorf Top Buret H

50 mL

11.2 Spare parts

Order no.

(International)

4965 612.004

4965 611.008

4965 616.000

4960 805.009

4960 851.000

4965 620.007

4965 625.009

Description

Discharge tube with knurled nut

Tube holder

Discharge valve with toggle

Telescopic aspirating tube

2.5 mL, 5 mL, 10 mL, 25 mL, 50 mL, 100 mL

Drying tube without drying agent

Air vent cover/filter connection

Battery

LR03/AAA, 1.5 V, 2 pieces

Ordering Information

Top Buret M/H

English (EN)

11.3 Thread adapter

• GL – round thread DIN 138 for glass threads. Number indicates the maximum outer diameter of the male thread.

• S – buttress thread. Thread type for glass bottles seals which is to prevent them becoming loose unintentionally due to vibrations.

• NS – glass vessel with ground glass joint. 1st digit is the inner diameter of the roughened glass, 2nd digit is the length into the vessel.

Order no.

(International)

Description

4960 800.040

4960 800.139

4960 800.058

4960 800.120

4960 800.155

4960 800.147

4960 800.082

4960 800.090

4960 800.104

Bottle thread adapter

PP from GL 32 to GL 25, from GL 32 to GL 27 from GL 32 to GL/S 28 from GL 45 to GL 32 from GL 45 to GL 38 from GL 45 to S 40 from GL 32 to NS 19/26 from GL 32 to NS 24/29 from GL 32 to NS 29/32

Ordering Information

Top Buret M/H

English (EN)

Certificates

Evaluate Your Manual

Give us your feedback.

www.eppendorf.com/manualfeedback

Your local distributor: www.eppendorf.com/contact

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany [email protected] · www.eppendorf.com

Key features

Continuous, pulse-free dispensing technique
Suitable for liquids with a concentration of up to 1 mol/L
Digital display for precise volume readings
Easy venting to remove air bubbles
Adjustable calibration for different liquids
Includes bottle thread adapters for various bottle sizes

Frequently asked questions

The Top Buret can dispense liquids with a maximum concentration of 1 mol/L.

Deair the dispenser by rotating the discharge valve toggle to the deairing position and rotating the dispensing wheels until no more air is present.

Clean the dispenser before a change of reagent, prior to storage, if it has not been used for some time, before maintenance and repairs, daily if concentrated alkaline solutions were used, daily if solutions which are prone to crystallization were used and weekly if the dispenser has been mounted to the storage bottle for some time.