BioMAT® 2 Safety Cabinet

BioMAT® 2 Safety Cabinet

BioMAT

®

2

Safety Cabinet

Contained Air Solutions Ltd | Unit 4 Greengate | Middleton Junction | Manchester M24 1RU |

T +44 (0)161-655-8860 | F +44 (0)161-655-8865 [email protected] | www.containedairsolutions.co.uk

We reserve the right to change specification without notice. © 2009 Contained Air Solutions Ltd

Section

Section 1

Your Manual

Section 2

Using your Cabinet

Section 3

Cabinet Control Panel

Section 4

Filter Replacement

Section 5

Service & Spares

3.1

3.2

3.3

3.4

3.5

4.1

4.2

4.3

4.4

5.1

5.2

5.3

5.4

2.1

2.2

2.3

2.4

2.5

2.6

2.7

2.8

1.1

1.2

1.3

1.4

1.5

1.6

1.7

Contents

Description

General Description

Quality Assurance/CE compatibility

Cabinet Siting

Installation

Avoiding Disturbances

Technical Data

Your Manual

Switches & Indicators

Start Up Procedure

Shut Down Procedure

Cleaning Procedure

Fumigation & Formalin Quantities

Fumigation Procedure

Airflow Measurements

Ultraviolet Radiation

Display

Alarm Circuits

Electrical Protection

Fan Speed Control

Engineers Menu

Downflow HEPA Filter

Re-circ HEPA Filter

Exhaust HEPA Filter

General Notes

Service Schedule

Spares List

Drawings (attached)

Wiring Diagrams (attached)

General Description – 1.1

Your BioMAT 2 Microbiological Safety Cabinet has been designed to provide optimum performance for both operator and product protection. Using the latest in microprocessor and fan technology, it is designed to exceed the performance requirements of the European

Microbiological Safety Cabinet Standard EN12469:2000. The safety cabinet carcass and all seals are maintained under negative pressure ensuring air cannot leak out during normal operation.

Class 2 Safety Cabinets offer both operator and product protection; they ensure any aerosols generated within the cabinet are filtered via high efficiency filtration (HEPA) prior to being dispersed back into the laboratory or to atmosphere. Operator protection is provided by an air curtain across the working aperture of the front screen. Protection of the products from external contamination is provided by a unidirectional downflow of sterile air into the working area.

The Class 2 Microbiological Safety Cabinet can be supplied as standard in either of the following two modes:

Ø

Re-circulating Type – Exhaust air from the safety cabinet is passed through two high efficiency filters (HEPA) before being released back into the laboratory. The exhaust air is made up of approximately 40% of the total air volume handled by the safety cabinet. Approximately 60% of the air is re-circulated within the safety cabinet and passes through a high efficiency (HEPA) filter to ensure sterility within the work area.

Ø

Exhaust Type – Exhaust air from the safety cabinet is passed through a high efficiency filter (HEPA) before being extracted to atmosphere through a fan assisted extract system. The exhaust air is made up of approximately 40% of the total air volume handled by the safety cabinet. Approximately 60% of the air is re-circulated within the safety cabinet and passes through a high efficiency

(HEPA) filter to ensure sterility within the work area.

Quality Assurance – 1.2

Although fully tested before leaving our factory as part of the ISO 9001:2008 Quality Assurance

Programme, the specified performance will only be maintained if your cabinet is sited correctly and regularly serviced. CAS can only accept responsibility for correct functioning of your cabinet if: -

Ø

Safety Cabinet is correctly sited in the laboratory to avoid any adverse conditions within the room that may affect the level of operator protection.

Ø agents.

It has been installed and commissioned by CAS trained personnel or approved CAS

Ø

Extension, modification, relocation, repairs or other maintenance is carried out by

CAS personnel or persons authorised by CAS or, in the case of electrical work, by qualified electricians.

Ø used.

Ø

In the case of repair or maintenance, replacement parts supplied by CAS must be

The electrical installation surrounding the unit and to which it is connected comply with the latest IEC regulations.

Ø

The unit is used and maintained in compliance with the instructions contained this manual.

in

CE Declaration of Conformity

CAS declares that the equipment supplied conforms to the following CE directives—

Machinery 2006/42/EC

Electro Magnetic Compatibility 2004/108/EC

Low Voltage 2006/95/EC

Cabinet Siting – 1.3

The siting of your Safety Cabinet is extremely important. Air currents and the movement of people in the laboratory can adversely affect the performance.

Ø

Ø

Safety Cabinets should be sited away from;

Ø

Doors and windows which open

Ø

Draughts caused by ventilation and air conditioning units

Pedestrian traffic routes

Other safety cabinets or fume cupboards

Ø

Adjacent fridge & Incubator doors

These points are of particular relevance to class 2 safety cabinets. The diagram shown below indicates some suggested locations for the correct siting of safety cabinets and highlights some situations which should be avoided.

3 4

SUPPLY GRILLE

2

HORIZONTAL

LAMINAR FLOW

Position 1

Position 2

Position 3

-

-

Position 4

An acceptable site not affected by disruptive air currents

Well sited

-Poorly sited if windows open – If not it should be a safe distance from the cabinet opposite.

-Poorly sited – can be affected by air currents from the opening door, through traffic and the horizontal laminar flow workstation sited directly opposite.

Installation – 1.4

Safety Cabinets are sophisticated items of equipment containing delicate filters which require expertise in their safe handling and installation into laboratories.

For exhaust type safety cabinets the exhaust ductwork route should ideally be surveyed and ductwork installation be carried out by qualified engineers as it forms an integral part of the system relating to the overall performance of the cabinet and is required to conform to various safety standards.

A poorly installed cabinet may compromise the protection provided by the cabinet to both personnel and work being handled and may present a hazard to other occupants of the building and the public.

·

Make-up Air

It is important that any make-up air compensating for the air exhausted from the safety cabinet does not cause draughts to the discomfort of the laboratory staff or detriment of the cabinet performance.

Air supply diffusers should be positioned more than 1500mm away from the front of the safety cabinet and have a maximum velocity of no more than 0.30m/sec.

·

Commissioning

When any safety cabinet is installed, it is necessary to carry out a number of commissioning checks in order to ensure it is fully operational and that the performance on site satisfies the current standard BS EN 12469:2000. This includes measuring the airflows, testing the HEPA filters with a suitable challenge aerosol and a KI Discus Test (operator protection test) to assess the containment of the cabinet.

CAS employs a team of fully trained installation and commission engineers to carry out all work necessary. This ensures that all new safety cabinets operate to the desired performance.

·

Site Surveys

If you have any queries regarding the siting of your safety cabinets we will be only too pleased to arrange a site survey by one of our regionally based technical support staff.

·

Periodic Maintenance & Servicing

To maintain safety cabinets at their optimum level of performance and to ensure lifetime operation, regular servicing is necessary. CAS provides a full servicing and maintenance scheme tailored to suit your individual needs. For more information on this please contact our service department on 0161-655-8860.

Avoiding Disturbances – 1.5

The sketches below show recommendations for avoiding disturbances for both cabinet operator and safety cabinet performance.

1000

Keep pedestrians away from the front of your safety cabinet

1500

Position clear of bench opposite

Keep clear of adjacent wall

C

1500

Position well away from the door openings

Keep clear of structural columns

2000

Keep well away from opposite wall

Technical Data – 1.6

Cabinet Size

Dimensions

External Dimensions

Internal Dimensions

Work Tray

Opening to Work Area

Weight Typical

Loading Capacity

Work Surface

Air Volumes Typical

Exhaust Air Volume

Re-circ Air Volume

Pressure Drop Typical

Exhaust Clean Filters

Exhaust Dirty Filters

HEPA Filter Data

HEPA Type

Efficiency

Heat Gains Typical

Exhaust

Re-circ

Noise Typical

Noise Level

Light Typical

White interior

St/St Interior

Duct Connection (Exh)

Electrical Data Typical

Voltage

Frequency

Phase

Power Consumption

Current

Internal Socket(s)

(w/d/h)

(w/d/h)

(w/d)

(w/h)

Kg

Kg m³/sec m³/sec

Pa

Pa

@ 0.3µ

Watts

Watts dB (A)

Lux

Lux

Mm

Volts

AC

Hz

N/A kW

A

900mm

900/640/1400

760/550/700

720/400

710/200

180

50

0.09

0.130

400

550

H14 (EN1822)

99.999%

190

100

<58

1000

800

160

230

50

Single

0.19

3

230v /13 Amp

1200mm

50

0.130

0.190

400

550

H14 (EN1822)

99.999%

190

100

<58

1000

800

160

230

50

Single

0.19

3

230v /13 Amp

1500mm 1800mm

1200/640/1400

1060/550/700

1020/400

1010/200

230

1500/640/1520

1360/550/700

1320/400

1310/200

280

1800/640/1520

1660/550/700

1620/400

1610/200

330

50

0.160

0.240

400

550

H14 (EN1822)

99.999%

270

135

<58

1000

800

200

230

50

Single

0.27

5

230v /13 Amp

50

0.190

0.290

400

550

H14 (EN1822)

99.999%

350

175

<58

1000

800

200

230

50

Single

0.35

7

230v /13 Amp

Your Manual – 1.7

This user manual has been prepared to provide a basic operating and maintenance instruction.

It is intended to supplement existing in-house procedures and codes of practice, and not to replace them. If further advice is required on the use or maintenance of this equipment, the staff of Contained Air Solutions Ltd. will be pleased to assist wherever possible.

Switches & Indicators – 2.1

The diagram below shows a typical control panel membrane layout for the BioMAT 2 safety cabinet.

BioMAT 2

CLASS 2 MICROBIOLOGICAL SAFETY CABINET

COMPLIES WITH BS EN 12469:2000

0

1

AIRFLOW SAFE

BioMAT 2

DESCRIPTION

A – Cabinet Operation

B – Lights

C – Gas Valve (Optional)

D – Alarm Mute

E – Fumigate

F – UV Lights (Optional)

G – Key Switch

LEGEND

0

1

COLOUR

Green

FUNCTION

Starts / Stops power supply to fans and control circuits.

Yellow Controlling power to lights.

Black

Red

Orange

Blue

Grey Border

To activate solenoid Gas Valve.

Will only function when cabinet is in safe working condition.

To mute audible alarm. Fault indication on display will remain until fault is rectified.

To activate fumigation cycle. Will only operate when cabinet is switched OFF.

To activate power to UV Lights.

Will only operate when cabinet and lights are switched OFF.

Key switch for supervisor control, used to prevent cabinet operation during fumigation.

Start Up Procedure – 2.2

The following notes are for guidance where local laboratory instructions do not exist or are inappropriate. They should complement, not replace, existing codes of practice issued by your

Laboratory Safety Officers.

Ø

Ensure power supply to the cabinet is switched on, as evidenced by the main display being illuminated. The key switch on the front of the cabinet must be turned to position ‘1’ =

(ON).

Ø

Press the green switch ‘A’ on the control panel – this will energise the exhaust fan followed by the downflow fans. The main display will show `AIRFLOW STABILISING` for 60 seconds, once airflows are settled the display will show `AIRFLOW SAFE`. On start up the audible alarm will sound until airflows are safe; this may be muted using the Alarm Mute switch `D’.

NOTE: Should the airflows change significantly and fall out of the specifications laid down in EN12469:2000 cabinet alarms will be automatically activated.

Ø

Switch on the interior lighting using switch ‘B’. Lighting can be activated when the cabinet is OFF; this can be used if loading or un-loading the cabinet with equipment prior to use.

Shut Down Procedure – 2.3

Ø

The work area should be cleared of any apparatus / equipment and cleaned in accordance with laboratory codes of practice. The cabinet should be left running for a few minutes to clear any residual aerosols.

Ø

Switch off the interior light using switch `B`

Ø

Switch off the cabinet using switch `A`.

Ø

Place the closure panel / Night door over the working aperture once complete.

Cleaning Procedure – 2.4

Regular cleaning is very important to prevent the build-up of dirt and hence potentially infectious material. Routine swabbing of work surfaces with 70% v/v IMS (ethanol) or IPA

(Isopropyl Alcohol) is recommended.

For cleaning the work surfaces, swabbing with a mild detergent in warm water is very effective. Phenolic or Cresolic disinfectants should be avoided as they may stain the white surfaces with a brownish colour. If they are used, any spillage should be quickly rinsed with clean water and mopped up with an absorbent tissue. Most of the quaternary ammonium compounds and the Glutaraldehyde based surface disinfectants are suitable.

To facilitate cleaning of the work zone and the interior, the whole front screen / visor may be opened and lowered from the top, supported by the hinges at the lower edge. It is good practice to clean the inside of the viewing screen to ensure adequate visibility of the working zone. Always consult the Laboratory Safety Officer before carrying out this procedure.

If Hypochlorites are used to clean the stainless steel interior of the safety cabinet they will initially cause rust spots and over time may lead to further damage.

Warning

Fumigation & Formalin Quantities – 2.5

When handling hazardous materials the air space inside the cabinet should be decontaminated regularly and always before servicing and following any spillages. Fumigation by formaldehyde gas is the recommended decontamination procedure for biological hazards although there are alternative methods available including VHP decontamination and Ozone decontamination.

To facilitate the fumigation a sequence has been incorporated in the cabinet controls, this is detailed under fumigation procedure.

A convenient way of generating sufficient formaldehyde is to boil off Formalin (40% formaldehyde BP or equivalent) in a suitable vessel such as a formalin vaporiser. These are available from CAS.

Always consult your Laboratory Safety Officer prior to fumigation of a safety cabinet.

** If in doubt ask **

Formalin Quantities

The recommended quantity stated in BS EN12469:2000 on Page 40 Annex J, Part 2 is 60ml formaldehyde solution mixed with 60ml distilled water per cubic meter of cabinet volume.

However this quantity is now considered in excess of that required to achieve a satisfactory kill.

We have therefore produced the following table based on quantities employed by users of large numbers of Safety Cabinets.

If you still consider that the quantities recommended in the British Standard are to be used, you may find on completion of the sterilisation cycle that high quantities of fluids containing formaldehyde are present in the cabinet.

Cabinet Size

Formalin

Distilled Water

900mm

15ml @40%

15ml

1200mm

20ml @ 40%

20ml

1500mm

25ml @ 40%

25ml

1800mm

30ml @ 40%

25ml

Fumigation Procedure – 2.6

Ø

Switch off the cabinet fans by pressing the green button ‘A’ on the control panel.

Ø

For re-circulating type safety cabinets the fumigation extract kit (optional) should be fitted to the cabinet discharge and ensure that the manual damper is fully closed. For cabinets connected to a duct system proceed as follows:

§

Fill the formalin vaporiser with the correct amount of Formalin (see section 2.5 for quantities) and screw on the aluminium cap – finger tight, having checked the gasket in the cap is undamaged. If the vaporiser is free standing, place on the cabinet work tray, plug into cabinet internal socket and switch on. Now fit the closure panel / night door, if using a lightweight closure panel additional sealing tape will be required around the outside face to ensure no leakage during fumigation. It is advisable to use a non permeable type tape that will not leave a residue once removed.

§

Now press the fumigation button `E’ and the display will show `FUMIGATION IN

PROGRESS’. A countdown timer will be shown directly below in minutes e.g. `285 MINS`.

During the first hour of the fumigation cycle the internal fans (downflow fans) will cycle for 5 seconds at 20 minute intervals, this ensures formaldehyde is well dispersed within the cabinet.

Ensure all laboratory personnel are aware that the fumigation is taking place; appropriate warning notices should be put on all doors entering the laboratory and on the cabinet being fumigated.

Warning

Ø

The cabinet should be left for a minimum of 6 hours, preferably overnight.

Note: Once the ‘fumigate’ button has been activated DO NOT SWITCH CABINET ON until the cycle has been completed.

Ø

On completion the cabinet will display `FUMIGATION COMPLETE` and `READY TO

VENT`. When ready, press the fumigation button to exit the cycle and then press the green button `A` to purge the cabinet of formaldehyde gas.

For re-circulating type safety cabinets press the ‘fumigate’ button to complete cycle and open the manual shut-off damper on the fumigation adaptor kit (Optional) having first fitted the extract tubing and placed the discharge point in a location approved by the

Laboratory Safety Officer.

** When fumigating a re-circulating type safety cabinet where venting is unobtainable, a mobile carbon filter unit may be required. **

Ø

Switch on the cabinet and then remove the sealing tape. To avoid formaldehyde being drawn out of the cabinet remove the rubber bung when using a 100% sealing closure panel.

Ø

Within the first few minutes of purging, the majority of the formaldehyde gas within the safety cabinet will be removed. However due to the fact that formaldehyde adheres to the surfaces of the cabinet and within the media of HEPA filters, we recommend that the cabinet be run continuously for at least 6 hours before the cabinet is serviced or work re-commences.

Ø

Remove the (optional) fumigation kit from the cabinet discharge before commencing to use the cabinet.

** Any poly-formaldehyde residue in the vaporiser may be removed by heating with water containing a little mild detergent at neutral pH.

General Note

Other methods of generating formaldehyde and other methods of cabinet decontamination can be employed; prior to using alternative methods your Laboratory Safety Officer should be consulted. Contained Air Solutions will be pleased to advise if they are able, but a detailed knowledge of every technique cannot be guaranteed.

If your cabinet is fitted with ports to allow fumigation using Vaporised Hydrogen

Peroxide (VHP) please refer to the VHP equipment manufacturer’s manual for the correct application of this technique.

Airflow Measurements – 2.7

It is recommended that the downward airflow be checked regularly, i.e. weekly or monthly according to cabinet usage. A rotating vane type anemometer with a head diameter of about

100mm is considered satisfactory. On 1200mm wide cabinets, take 8 readings, namely four along a line one quarter of the depth of the working space forward of the rear wall, and four along a line the same distance behind the front window on a horizontal plane 100mm above the top edge of the working aperture. Anemometer positions for the 1200mm & 1800mm sizes of cabinet are illustrated below. The results should be recorded to monitor cabinet performance over time.

1/4

1/8 1/4 1/4 1/4 1/8

1/2

1/4

1200mm wide

1/12

1/4

1/6

1/2

1/4

1/6 1/6 1/6 1/6 1/12

1800mm wide

Unidirectional Downflow Air

NOTE: - The unidirectional downflow air within the cabinet is set up during commissioning to give an average velocity of 0.3 to 0.35m/sec measured at a horizontal plane 100mm above the top edge of the working aperture. The limitations laid down in the standard are minimum 0.25m/sec - maximum

0.50m/sec with no one reading being greater than +/- 20% of the mean velocity.

This preset velocity can only vary if (a) the soiling of the downflow filter occurred, or (b) should there be a fan failure.

NOTE: - We recommend that the downflow velocity be checked on a regular basis and the results recorded to monitor cabinet performance over time.

Ultraviolet Radiation (Optional) – 2.8

Ultraviolet Radiation (UV) lamps may be fitted as an optional extra; these can be fitted as new in our factory or retrofitted at a later date on site. If installed the cabinet will have 2 short wavelength

Ultraviolet (UV) tubes emitting 254 nano metres fitted to the inside work area of the safety cabinet.

As a safety feature the UV tubes are interlinked with the cabinet lights to prevent them being used when the cabinet is in use.

Over time, the effective life of UV tubes is known to reduce; therefore we would recommend UV tubes are replaced on an annual basis to ensure maximum efficiency.

Applications

Many bacteria are quite resistant to UV Radiation, and may require prolonged exposure for sterilisation. Dry and/or protein covered organisms may be protected against UV and may be only slightly affected if at all. However, moist, vegetative cells without too much protein covering are killed with reasonable effectiveness after 3-4 hours exposure.

Warning

Extreme care must be exercised when using UV radiation. Consult your Laboratory Safety Officer prior to use.

UV Radiation can cause burns to unprotected skin and it is very important not to look directly at the illuminated tubes with the naked eye.

Display - 3.1

The cabinet display is located in the centre of the control membrane; it incorporates backlighting to ensure all signs can be clearly seen from the operating position.

Typical display during normal operation shown below:

AIRFLOW SAFE

BioMAT 2

In the event of an alarm condition the display will clearly indicate the fault; this will remain displayed until the fault is rectified.

Typical display during alarm condition shown below:

DOWNFLOW FAIL

Should the cabinet produce an alarm condition it may be necessary to arrange for a Service

Engineer to attend site, in such cases please contact our Service Department on 0161-655-

6183.

Alarm Circuits – 3.2

There are three standard alarm circuits on the BioMAT 2 Re-circulating and Exhaust type Safety

Cabinets.

1. INFLOW

Sensed by an accurate pressure sensor mounted on the main printed circuit board and connected via tubing to the sensing points within the cabinet body, this will sense low airflow caused by fan failure or filter soiling, and transmit the signal to the display meter on the front panel and alarm circuit. In the event the alarm is triggered the display will show `LOW

AIRFLOW`, and the audible alarm will sound, this can be muted using the alarm mute button.

1.

DOWNFLOW

Sensed by an accurate pressure sensor mounted on the main printed circuit board and connected via tubing to the sensing points within the cabinet body, this will sense low airflow caused by fan failure or filter soiling, and transmit the signal to the display meter on the front panel and alarm circuit. In the event the alarm is triggered the display will show

`DOWNFLOW FAIL` and the audible alarm will sound, this can be muted using the alarm mute button.

2.

FRONT SCREEN

Front screen position is monitored by a micro-switch fitted to the front screen assembly. When opened the display will show `VISOR OPEN’ and an audible alarm will sound.

This can be muted using the alarm mute button.

** The alarm circuit is activated each time the cabinet is switched on.

It is important to ensure that the pressure tubing is not damaged or split where it is connected to the pressure sensor. The pressure sensor, in the event of inadequate airflow in either part of the system, will cause the alarm circuit to be activated with a flashing red indicator on the alarm mute button, an intermittent audible alarm tone, and the alarm system will clearly show on the display. The audible alarm tone can be silenced by depressing the ‘alarm mute’ button on the control panel after which the red light will remain continuously lit until the correct airflow is reinstated or the cabinet is switched off.

Electrical Protection – 3.3

Ensure the Safety Cabinet is isolated from the mains supply prior to opening access panels.

Warning

Fuses

There are 11 fuses mounted on the printed circuit board to protect the electrical circuits of the cabinet. The cabinet controls are housed behind the front control panel which is secured with two screws located across the bottom edge. Once removed the control panel will hinge upwards to facilitate access to the main printed circuit board.

For full details on fuse ratings and the circuits they protect please see the wiring diagram attached to this manual.

Fan Speed Control – 3.4

Both the inflow and downflow fans can be adjusted via the engineer’s menu please see section

3.5 for more information.

To maintain optimum performance during routine planned maintenance it may be necessary to increase cabinet fan speeds to overcome filter soiling. This can be adjusted via the front control panel membrane using the Engineer’s Menu.

Warning

It is important any changes to fan speeds must be made by a CAS service engineer or alternative competent service provider, failure to do this may result in the warranty being invalid.

Engineer’s Menu – 3.5

Warning

It is important any changes within the Engineer’s

Menu be conducted by a CAS service engineer or alternative competent service provider; failure to do so may result in the warranty being invalid.

The buttons located on the front control membrane have a second function once access to the

Engineer’s Menu has been gained.

** In order to gain access to the Engineer’s Menu the cabinet must be switched OFF **

STANDARD SWITCH PANEL ARRANGEMENT

SOFT BUTTON ARRANGEMENT

TO ENTER `ENGINEERS MENU` BUTTON 1 SHOULD HELD DOWN FOR >5 SECONDS

TO EXIT `ENGINEERS MENU` BUTTON 1 SHOULD HELD DOWN FOR >5 SECONDS

USE BUTTON 2 TO START / STOP THE FANS

Engineer’s Menu Sequence

LOW ALARM

40 Pa

YES

NO

HIGH ALARM

80 Pa

YES

NO

DOWNFLOW ALARM

120 Pa

YES

NO

INFLOW FAN

65 %

YES

DOWNFLOW FAN

40 %

NO

YES

U.V. LIGHTING

060 MINUTES

NO

YES

NO

TIME ADJUST

14:54

YES

NO

EXHAUST SENSOR

20 Pa

YES

NO

SERVICE TIMER

RESET

YES

NO

INITIALISE VALUE

INITIALISE (0)

YES

Downflow HEPA Filter – 4.1

The downflow filter is a single HEPA filter mounted immediately above the working area.

Access is gained by opening the control panel door.

IMPORTANT

Cabinet must be decontaminated prior to changing any HEPA filters, see section 2.6

Only replace or examine filters if authorised to do so by the Safety Officer or the person in charge of the laboratory. To remove any filters wear disposable gloves, an apron, overalls and appropriate face covering, especially eye protection.

Ensure the Safety Cabinet is isolated from the mains supply prior to opening access panels.

Warning

The front control panel is hinged at the top and retained by fixing screws in the lower edge, when opened it can be supported by two stays. Behind the front control panel is the main access panel held in place by several screws. Care should be taken to ensure the panel is supported prior to releasing the last screw.

Behind the access panel is the downflow fan module and the downflow HEPA filter. Turning the jacking screw clockwise at each side a little at a time until free from the filter will raise the fan module. The downflow filter may then be drawn forward and out of the cabinet body, this can then be disposed of as recommended by site regulations.

To replace, first ensure that all surfaces have been cleaned and are free from matter.

Carefully remove the new filter from its protective packing.

Inspect both exposed surfaces for signs of damage. Any damage should be reported to the supplier immediately.

The filter should be slid carefully into the cabinet taking care not to damage the gaskets, if necessary by supporting the filter from within the work area. The fan module is then carefully lowered by turning the jacking screws anti-clockwise a little at a time so as to compress the filter gasket to approximately half its original thickness. The panels may then be replaced, and the filter and its gasket tested for leaks by challenging with DOP or equivalent aerosol and scanning the surface with the probe of a forward light scattering photometer.

Re-circ HEPA Filter – 4.2

The double HEPA filter is located on the top of the main body of the cabinet held in place by two compression fasteners.

IMPORTANT

Cabinet must be decontaminated prior to changing any HEPA filters, see section 2.6

Only replace or examine filters if authorised to do so by the Safety Officer or the person in charge of the laboratory. To remove any filters wear disposable gloves, an apron, overalls and appropriate face covering, especially eye protection.

Ensure the Safety Cabinet is isolated from the mains supply prior to opening access panels.

Warning

To remove filter, release fasteners, lift module until filter can be removed. Slide filter forward and out and dispose of as recommended by site regulations

To replace, first ensure that all surfaces have been cleaned and are free from matter.

Remove new filter from its protective packing.

Inspect both exposed surfaces for signs of damage. Any damage should be reported to the supplier immediately.

Slide filter carefully into position. Lock fasteners, ensuring filter is correctly positioned and gaskets are compressed to about half their original thickness.

The filter and its gaskets should be tested for leaks by challenging with DOP or equivalent aerosol and scanning the surface with the probe of a forward light scattering photometer.

Exhaust HEPA Filter – 4.3

The single HEPA filter is located on the top of the main body of the cabinet held in place by either two compression fasteners or jacking screws.

IMPORTANT

Cabinet must be decontaminated prior to changing any HEPA filters, see section 2.6

Only replace or examine filters if authorised to do so by the Safety Officer or the person in charge of the laboratory. To remove any filters wear disposable gloves, an apron, overalls and appropriate face covering, especially eye protection.

Ensure the Safety Cabinet is isolated from the mains supply prior to opening access panels.

Warning

To remove filter, release fasteners or jacking screws, lift module until filter can be removed.

Slide filter forward and out and dispose of as recommended by site regulations

To replace, first ensure that all surfaces have been cleaned and are free from matter.

Remove new filter from its protective packing.

Inspect both exposed surfaces for signs of damage. Any damage should be reported to the supplier immediately.

Slide filter carefully into position. Lock fasteners or jacking screws, ensuring filter is correctly positioned and gaskets are compressed to about half their original thickness.

The filter and its gaskets should be tested for leaks by challenging with DOP or equivalent aerosol and scanning the surface with the probe of a forward light scattering photometer.

General Notes – 4.4

Ø

Do not store equipment inside the Microbiological Safety Cabinet. The amount of equipment should be kept to a minimum to reduce the disruption to the airflow patterns within the cabinet.

Ø

A Bunsen burner should not be used inside the cabinet.

Ø

Great care should be taken to prevent litter such as disposable gloves or tissues from being sucked through the airways at the rear of the work area. These can lodge and reduce the airflow through the cabinet. It is good practice to clean underneath the work tray regularly. Always consult the Laboratory Safety Officer before carrying out this procedure.

Ø

The failsafe solenoid operated gas valve (If fitted) will only allow gas to flow when the cabinet is switched on and there is a satisfactory airflow. Any interruption in the power supply or failure of air supply necessitates manual resetting of the valve by depressing the switch on the control panel marked ‘gas valve’.

Ø

As a safety feature the UV (If fitted) tubes are interlinked with the cabinet lights to prevent them being used when the cabinet is in use.

We recommend cabinet users to consult their own laboratory safety information. Other publications available include:

Science Reviews Ltd, Occupational Hygiene Monograph No.9

British Standard BS EN 12469:2000 & 5726-2000 Part 5

Australian Standard AS2567

American National Sanitation Foundation Standard No. 49

We would also recommend the latest guidelines issued by the UK Advisory Committee on

Dangerous Pathogens (ACDP). These documents contain much useful information on the performance, installation, testing, use and limitations of Microbiological Safety Cabinets.

Service Schedule 5.1

SERVICE SCHEDULE 5.1

Class II Microbiological Safety Cabinets

Schedule of work included in service agreement

6

7

4

5

8

9

1

2

3

Filter Integrity Test (D.O.P) on all main filter(s) and seals

Airflow Profiling ensuring compliance to latest British & European Standards

Operator Protection Testing (K.I. Discus) 1 x Per Annum

Check and adjust alarm parameters & control functions as required

Check and adjust electrical and electronic controls as related to the airflow system

Check and adjust operation of exhaust system as required

Mechanically inspect cabinet components and duct connections (if fitted)

Change main filters as necessary (Supply and fitting subject to additional charge)

Prepare computer generated Service Report

Above work to be carried out twice each service year

(Operator Protection Test (K.I. Discus) carried out once per year unless instructed otherwise

Service schedules can be tailored to suit individual needs

For a competitive Quotation please contact our service

Department on

0161-655-8860

Spares List 5.2

Shown below are ordering codes for the most common parts used on the BioMAT 2 Safety

Cabinets. In addition to these items we stock a vast range of Spares, consumables and optional extras. If you cannot see what you require please give us a call on the telephone number shown below.

** Please note cabinet serial number must be quoted when ordering **

UK:

Tel: 0161-655-8860

Fax: 0161-655-8865

Item

Downflow HEPA Filter

Exhaust HEPA Filter

Recirc HEPA Filter

Downflow Fan

Exhaust Fan

Main PCB

Control Membrane

Light Tube

UV Light Tube

Key Switch

Standard Closure Panel

100% Sealing Closure Panel

Formalin Vaporiser

Fumigation Adaptor Box (Recirc)

Overseas:

Tel: +44-161-655-8860

Fax: +44-161-655-8865

ORDERING CODES

BioMAT 2

1200mm

FIL 001

FIL 002

FIL 003

FAN 050

FAN 050

ELC 325

LAB 114

ELC 077

ELC 061

ELC 326

CCM 725

CCM 726

CCP 083

CCM 111

BioMAT 2

1500mm

FIL 025

FIL 167

FIL 117

FAN 050

FAN 057

ELC 325

LAB 114

ELC 097

ELC 061

ELC326

CCM 800

CCM 801

CCP 083

CCM 802

BioMAT 2

1800mm

FIL 005

FIL 048

FIL 007

FAN 050

FAN 057

ELC 325

LAB 114

ELC 049

ELC 061

ELC 326

CCM 772

CCM 773

CCP 083

CCM 112

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